LOCUS       DAJTVK010000001       109775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000001 DAJTVK010000000
VERSION     DAJTVK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 109775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.001"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..891
                     /gene="metG"
                     /locus_tag="OO372_000001"
     CDS             <1..891
                     /gene="metG"
                     /locus_tag="OO372_000001"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HCR6339628.1"
                     /translation="VNADIVNKVVNLASRNAGFINKRFDGVLAGELADPQLYKTFTDA
                     AEVIGEAWESREFGKAIREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMGIN
                     LFRVLMTYLKPVLPKLTERAEAFLNTELTWDGIQQPLLGHKVNPFKALYNRIDMKQVE
                     ALVEASKEEVKATAAPVTGPLADDPIQETITFDDFAKVDLRVALIENAEFVEGSDKLL
                     RLTLDLGGEKRNVFSGIRSAYPDPQALIGRHTIMVANLAPRKMRFGISEGMVMAAGPG
                     GKDIFLLSPDAGAKPGHQVK"
     gene            1053..1352
                     /locus_tag="OO372_000002"
     CDS             1053..1352
                     /locus_tag="OO372_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339629.1"
                     /translation="MLNITQAYQVALISSSHTSEEDARRFMIASNELDWVSQIDGGCI
                     VHAGLQDDVWKDDLRRYGISEGAIANIQKVLDVGFDSVHFDCGAPVVEGLECWNW"
     gene            1398..2132
                     /locus_tag="OO372_000003"
     CDS             1398..2132
                     /locus_tag="OO372_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339630.1"
                     /translation="MKKLKSLEIPGTGYKFFMDNAECAPNALEKIQSLINSLTEEAKE
                     LKSEATKDNTAKIQCLNLVSYCLYQVSKTLIKGNIYYDDEDTSTPEEKDSDEELYEDI
                     STSAGHAAFDAMGKSSGIRSFVEQNNSTIMIEYCEQTSSKAIHVLHDVAAGVRCEAEA
                     VRKDVFGADSNTVKAFINSVLLNSYSNLIVNAALCANSGEEHIVITETKHEHQQSPTT
                     EEVTALAIKQASEISDEILSLLNKTK"
     gene            3688..4017
                     /locus_tag="OO372_000004"
     CDS             3688..4017
                     /locus_tag="OO372_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339631.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMICDMGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     EFLEVFE"
     gene            4172..4483
                     /locus_tag="OO372_000005"
     CDS             4172..4483
                     /locus_tag="OO372_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339632.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWMAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            4610..5005
                     /locus_tag="OO372_000006"
     CDS             4610..5005
                     /locus_tag="OO372_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339633.1"
                     /translation="MTVKREKLTVDVYYASETAEGKNVAKITVVTYNTETGAEVQAST
                     IVRKGDASGGEYETQYQSIFDATDPLLLKIENYFRQVDEEVFEPMMNMVNTVFASNLN
                     TNTTWIGQYGLRITSGIPAATLIPESVFA"
     gene            5123..5497
                     /locus_tag="OO372_000007"
     CDS             5123..5497
                     /locus_tag="OO372_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339634.1"
                     /translation="MGLDIYIETQPKNDLNNEASRKQVAYFRKFNALVGWMERNVGEV
                     ENCELLELTMNDICFLKAHLMHINESNCEEYLPTREGFFFGSQEYDEGYWHDVGELKE
                     LVEDLIKNHDFHNNRLTFCAWW"
     gene            5833..6114
                     /locus_tag="OO372_000008"
     CDS             5833..6114
                     /locus_tag="OO372_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCR6339635.1"
                     /translation="MTDFTITPKAQNVFLESWLDLPETEQQEMDHVEYDEQVSTRFFH
                     FEGCVYDIADFMRDDRFPEWHASYPLNAFAMLMIRVDDSGDTIDIGLLH"
     gene            6319..6801
                     /locus_tag="OO372_000009"
     CDS             6319..6801
                     /locus_tag="OO372_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339636.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(7417..7647)
                     /locus_tag="OO372_000010"
     CDS             complement(7417..7647)
                     /locus_tag="OO372_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339637.1"
                     /translation="MFILIAGVNVRNEYFVNRIAGIAGYAGRAVELIDETTRKIDLLS
                     DQERKKADVNDADIFLMLKAFVEMGFEISLHK"
     gene            complement(7695..8345)
                     /locus_tag="OO372_000011"
     CDS             complement(7695..8345)
                     /locus_tag="OO372_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339638.1"
                     /translation="MTNIIISKKSIIEAAAIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKAGKCVYSVADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGAGSAKAGTQQYRFIKNDFYNYLADAFKA"
     gene            complement(8641..8940)
                     /locus_tag="OO372_000012"
     CDS             complement(8641..8940)
                     /locus_tag="OO372_000012"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339639.1"
                     /translation="MKTVAAFTFLTFIFSSSAFSQENSIERVQRVSTELCSKAEDYDL
                     CMLDLSMALGAAYQDGLIAAGCKLGLDEPSKDKCENSERFQEYIIKEIRNYAKKM"
     gene            complement(9316..9837)
                     /locus_tag="OO372_000013"
     CDS             complement(9316..9837)
                     /locus_tag="OO372_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6339640.1"
                     /translation="MRTLDITYGIPAEVWPRNYEHAERTLLFWRSAQFPVRVTLEDGQ
                     VFCMYVYGLLSARNKLNLTPDPFSNTNRVRLPLERISTIESGIEEVDTSFTGRLTLNN
                     EQMVNQPSRRDFFAICRKAYQNNQSIRVYMADGREIEGVSRGVDACQVTLKLEDGRKI
                     LVFFDWVERILPF"
     gene            complement(9834..10433)
                     /locus_tag="OO372_000014"
     CDS             complement(9834..10433)
                     /locus_tag="OO372_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339641.1"
                     /translation="MKMKLSLLLACICLPAFSANALSDFVSDMQKTYSMYTSEDVLKY
                     KSRGLIQPVWYRFSDNAEETILHAGKERLRLQINGTAVPENANVSSNEAMKPSGIVLE
                     IQDGTLYCDEMATTGCQVNIRVGNEEPIVTYAKTEGNTSGKVLFIDENTTKRIVRDIK
                     KTANSKSVDKKIYVEVPFLVVGNKEFKFGLLKTPLRVSE"
     gene            complement(10449..10937)
                     /locus_tag="OO372_000015"
     CDS             complement(10449..10937)
                     /locus_tag="OO372_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339642.1"
                     /translation="MSISINKIINSLRHKISHGKDVLFKDKYYLFNLVPYKCTKFYIA
                     RDCKYSVSITKDMKKISSEEYFISHIYIYPFKLEPLNIILPTIKGVELEGYTLSSNFN
                     DMGVQKPGARSYYAYKVKCNNYNALNMLISKLETEIVKGNLNDDHEKLEQIEKLRKCD
                     VK"
     gene            complement(11083..11682)
                     /locus_tag="OO372_000016"
     CDS             complement(11083..11682)
                     /locus_tag="OO372_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339643.1"
                     /translation="MMCSLVRKEHIMSWLKELSFVDTTGAKKNHSFWKVSDPSDFLVG
                     ANAAFELLNFYQRYPEMKHSPLLYRITNDMNRSGLLSSESAKGFFSKITPHISFDAAN
                     EIDYLSTKSQEQLSVPPEKKERRIASIENISKELRTTTLRLLNFLTLLNWIDDQTLIP
                     STDALDNRVLRLNKKSQFGFVFTKKGEDLIKRKYRLLND"
     gene            complement(11715..11993)
                     /locus_tag="OO372_000017"
     CDS             complement(11715..11993)
                     /locus_tag="OO372_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339644.1"
                     /translation="MSEKKTTYCQVALSDKANEKLGKFQIKLKEKKIKMSKAEVINAI
                     LETMTMAEFDKVTSAVGVSAKTREKIMRIYENSNMTKKDLEEILSRLP"
     gene            complement(11996..13555)
                     /locus_tag="OO372_000018"
     CDS             complement(11996..13555)
                     /locus_tag="OO372_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6339645.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVETGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAENGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRDLVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEVRIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQSTRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            13638..14318
                     /locus_tag="OO372_000019"
     CDS             13638..14318
                     /locus_tag="OO372_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6339646.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            14318..14986
                     /locus_tag="OO372_000020"
     CDS             14318..14986
                     /locus_tag="OO372_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR6339647.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     INREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEETTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            14983..15648
                     /locus_tag="OO372_000021"
     CDS             14983..15648
                     /locus_tag="OO372_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6339648.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHL"
     gene            15645..16535
                     /locus_tag="OO372_000022"
     CDS             15645..16535
                     /locus_tag="OO372_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339649.1"
                     /translation="MTDIIIKRYRPEEFPRHLDFLERMTVTKGTVEDWHALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            16545..16811
                     /locus_tag="OO372_000023"
     CDS             16545..16811
                     /locus_tag="OO372_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339650.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            17007..17639
                     /locus_tag="OO372_000024"
     CDS             17007..17639
                     /locus_tag="OO372_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6339651.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGSEGEDE"
     gene            17639..18895
                     /locus_tag="OO372_000025"
     CDS             17639..18895
                     /locus_tag="OO372_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR6339652.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRKWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQITVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMTASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            18922..20496
                     /locus_tag="OO372_000026"
     CDS             18922..20496
                     /locus_tag="OO372_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6339653.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGAHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMQLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDQGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            20518..21405
                     /locus_tag="OO372_000027"
     CDS             20518..21405
                     /locus_tag="OO372_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339654.1"
                     /translation="MQLWQMLLARRGLMDVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTHEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYAALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLASGLGKIGLK"
     gene            21431..22306
                     /locus_tag="OO372_000028"
     CDS             21431..22306
                     /locus_tag="OO372_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR6339655.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREKTLSEATFIDVNDTIPEGAATFEECVAKLRILAGDVDVDKFLATTMADTNNQL
                     AIQVRQKVKGLARAFRRNLILGDSSSNNKAFDGIPRLMHADQKIDIAGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGVPFIIND
                     FIPTDSGKASIYCLHLSEENGVTGLYGGDNAGIVVENIGTVQNKDAVRTRVKWYCSLA
                     NKHDKAIAALTNVKI"
     gene            22380..23300
                     /locus_tag="OO372_000029"
     CDS             22380..23300
                     /locus_tag="OO372_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR6339656.1"
                     /translation="MPEQKMKITEEAFSDFTGHMCRAGFTNSISDEPLTERQQSQLSA
                     CLQAVPLSQSVNITPASPSVLVGKTVQLSAGISMGKSASSFTWKSANDQIATVNGTGL
                     VTGVAPGKVKITATDQETQLSASVEVTVNPVAVQSVTVTPDSTSVEKGKSVSLKANVQ
                     PSNATNKAVTWSSKNEDKATVDQSGNVTGVEVGTATIEIVSQDGSKKATATVEVTAPV
                     VAVTGVEIDPNSTTVEANKTVQLTANVEPAGATNKTVTWESKNTEFATVDSETGVVTG
                     VAAGTATIEVTTQDGSHKATATVEVTAAQE"
     gene            23345..23779
                     /locus_tag="OO372_000030"
     CDS             23345..23779
                     /locus_tag="OO372_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339657.1"
                     /translation="MKPAKIVLLEPQFSGYSGMLCGVQFENGVSVAELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYSDRGELTADLITEPAAPDIVPMKRGTPDEPAKQIQTFTRE
                     ELESIADNEGIAGLRVIGNQVGVKAKGIVEMIEGILKAQGGE"
     gene            23779..24612
                     /locus_tag="OO372_000031"
     CDS             23779..24612
                     /locus_tag="OO372_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339658.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEITSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNSFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDVAAAREDGILSESIGETTNMYRTGKAANVHVARSTWRLLVSYINNRMIVRR
                     A"
     gene            24692..25036
                     /locus_tag="OO372_000032"
     CDS             24692..25036
                     /locus_tag="OO372_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339659.1"
                     /translation="MNISWQTEIAIYRFGAKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLEAAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            25027..25500
                     /locus_tag="OO372_000033"
     CDS             25027..25500
                     /locus_tag="OO372_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339660.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIAGVKPRVGPHFLERAVEISEKQFSEAILEAARKAGFTRG"
     gene            25502..25885
                     /locus_tag="OO372_000034"
     CDS             25502..25885
                     /locus_tag="OO372_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339661.1"
                     /translation="MFIEAFASLMQKAKIGTVGADIFCHYLPANVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTVEKANKIIEMFPVEETESGGVYFRLVRPMAMP
                     IIYPKNDGALIEAGIPVEFAGYLLN"
     gene            25960..26706
                     /locus_tag="OO372_000035"
     CDS             25960..26706
                     /locus_tag="OO372_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCR6339662.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QLGQTVISELVQGRNITITAPLAESVLQNMVDLMPGSTLSEDDNSVTITSAQGVNLID
                     VAKELVLTPQDTTDYVLTIPKAATAGNFTMTYQSDDVRVFSVQFSAYPDDEGVLGKMS
                     GPKPVKTVSISPESPTVKAGETVQLTAEITPADAADKTGVWESEDQEKATVDQTGLVR
                     GVAQGSTNISFTSNSGGKKATKSVTINPAD"
     gene            26762..27079
                     /locus_tag="OO372_000036"
     CDS             26762..27079
                     /locus_tag="OO372_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339663.1"
                     /translation="MTKLLDLDSILPPKKSIKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LMNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            27205..27429
                     /locus_tag="OO372_000037"
     CDS             27205..27429
                     /locus_tag="OO372_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339664.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARASQADGEAVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDKADPDAKEQLMQIFGRG"
     gene            27437..31984
                     /locus_tag="OO372_000038"
     CDS             27437..31984
                     /locus_tag="OO372_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6339665.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASASVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLISKPVRVRTSGSGDGGSGQRS
                     GASGHSGKNNEGGMFSGLRGNIFLLGEIGDAARTVTDIMFGWQKPIVEAAAEMERMRV
                     MLRGLNKEKSNPGQAAADDMKYIVDMAKSAPFAMEALTDSFVKFRSAGLDPTDGSLKT
                     LVDSVARFGGDSELLKRASIAIQQMSGKGVVSMEELRQQLGEAIPTAMNAMAKAAGLT
                     MGELTKAVASGTVEAKNALALMFKELKMQNEGAASEMMNTFTGTLAQMQTAFTLFADK
                     VGQAGYLSSLKEGMKELTSLMNSTDGMSFAHSLGSGLTTAVDGLRDLAMWLIKNQELV
                     IAFGKVVAAMVAFKLLKAGISGVIGTASQMTSAFANMSTTIQGTFNLASTAVKRFNRA
                     ASMGLSPIPSLIFAIRNAFSGLVAFMAANPIGFAFTAAVTAVSSLIFYMQVLRGKIED
                     VVNKVREIPEAMTAADRAMLESAKREKDRQISKLEKEVTTGKRSQVSRDGLVYETDVD
                     VNSTKQQLENLKKERDDIAKTLGEGIEKSVYKSVTKGLDDAIAEVNQKANVSLVANAK
                     ELEQLNKKNEEIAGRTDLSDHEKSQLRAANNAKIERLYSEADLQKLKGYQDIQKGILN
                     DLVNLNEDFKKATTANNTEQLKEIQARIDAQIANKKSIEEQIENVTTSLSVKAGSGLK
                     SINGGKIGEFSSTPVDANSKTMTGFWKQQMDSKRYQRVNLDGTALYDLQGQPVVGPRQ
                     LKEQLRQKKIYGDVKLEQLSENDRIAVIKTLTKARELDAEAADKAANRTAKAAERAAK
                     KEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLTDLANAVPNE
                     FITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSIISAGYTPSREE
                     KVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIKETGTATQKL
                     ALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDITNMVVKSQIT
                     LPLMNMLGMGATAAGSSQSGNLLTGVASAVANQGVRISNTVNGDKSVGEATKETSSSV
                     TGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSMQNLSSVTGA
                     LSAAFAMLGANASGSGNKWLNFGATVASGLVSVWAGGGFDGLMSGSSGSTSGFNNLTS
                     SAADGTNGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGDMNEAYVPL
                     PDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNAAQRMKAIA
                     LETIAQEKRPGGSLNPNTQRN"
     gene            32027..32362
                     /locus_tag="OO372_000039"
     CDS             32027..32362
                     /locus_tag="OO372_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6339666.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            32445..33143
                     /locus_tag="OO372_000040"
     CDS             32445..33143
                     /locus_tag="OO372_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6339667.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            33136..33933
                     /locus_tag="OO372_000041"
     CDS             33136..33933
                     /locus_tag="OO372_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6339668.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDIFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            33921..34514
                     /locus_tag="OO372_000042"
     CDS             33921..34514
                     /locus_tag="OO372_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6339669.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVVMPALAPLGLSLMMGGISQIISPQATNESVRQADNSNSYYFD
                     GPQNTENQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            34533..39260
                     /locus_tag="OO372_000043"
     CDS             34533..39260
                     /locus_tag="OO372_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HCR6339670.1"
                     /translation="MEQFKKKKLPLFIAGAGGKKSSKSSSRTPVEADDTVNSRAMAAI
                     LDLLGEGVIGGLVNGARSIFVDDLPIVNEDGSSNFSGITWDFRDGSQDQTPMSGFDFV
                     ETPKSVNIQLKKTHYVTVSIDNDEADRVRVIMKFPSLRSIDKKTGDTNGTTVEYKFQI
                     ANGDSTFVDVVAEGEKSVGIKLTAKKTGVYYRSYELKLPKPGRAYKVRVVRITDDNSS
                     QYLYNDTWVDSIGEIVDTPMNYPNSVLVGLKVNSEQFGSTMPSRSYLVRGLKIRVPSN
                     YNEASNTYDGVWDGTFKLLSSSNPAWILFDLLTNARYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVAFTKNSVTLDAPVSLAKGGCFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETPGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISTVYQVLGT
                     MTPPDAPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THAGLGDSETWYYWVRASNKRGMLSPPNSNLGTEATTRDVLSFLTGKITSSELGQALL
                     EDINSKASQEAVDELNEHINQSVESLEGSVNDVKEDIAELDKQFSDNLAGFEIKFNER
                     SDALENAQTELKGEVSATIDKVNEAFEKIDATDAAIVEIENTVSEHDKALANTVEAIK
                     AARDEAAALIAKESEARVEGDAANAKQLEVLQSTVEESSAAVEEMKKTVAEVDRASAE
                     LTTNIEALAKTNIDLALRQDEDQHKQMVNNAKIATTQKTFADDMSAMATKVEEIRVEI
                     GEDIKASILEESTARADGDEALAKRVTQLQSKFEGDISAAISTEQEARTSADEALTTQ
                     ITQLESKVSNDIVAAIKEEQEARATEDSALASQITQLQAKVDDDISAAIRSEQEARAS
                     GDSALAKQINQLQSKVDGDISAAITQEQEARASADQALSREINSLRAQTGTDIAAAVA
                     VETKARTDADSALSTQITSLTAKANDLEASLARETTARADGDTALTKEVSNLKAQTAK
                     DISAAVAVETQARTDADSALSSQITKLTSQYKEDIKAAVATETKTRTEQDAALATQIT
                     NLESQTAANISAAVTTETTARTQADNALSGRIDTLKAEVDGNTATIQQQATAIADTNK
                     KVSTAWTLKMETSTSGGQKYVAGIALGIDTTGLSQFLVQADRFGLVNSVNGKITTPFV
                     IENSIAYMNGAYIKDGTITNAKVGDLQSTNFVSGRSGWRFGKNGTLEINGNSGGNGRL
                     VINGQRIDVYDDNNVLRVRIGRL"
     gene            complement(39896..40189)
                     /locus_tag="OO372_000044"
     CDS             complement(39896..40189)
                     /locus_tag="OO372_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339671.1"
                     /translation="MAFVSDVFAAFAEFPAAVAEEAALLALKDAFVSDDFAASFEAAA
                     SRAEVAASDAFVVAVDAEVAADCCDAAAFVSDFFAAVALALAALCEDAAADAL"
     gene            40710..42038
                     /locus_tag="OO372_000045"
                     /pseudo
     CDS             40710..42038
                     /locus_tag="OO372_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            42570..43067
                     /locus_tag="OO372_000046"
                     /pseudo
     CDS             42570..43067
                     /locus_tag="OO372_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            43149..44411
                     /locus_tag="OO372_000047"
     CDS             43149..44411
                     /locus_tag="OO372_000047"
                     /inference="COORDINATES: protein motif:HMM:NF025253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HCR6339672.1"
                     /translation="MAAPSVQLRGQGTDTRQYIMAYRTDGATSWYVGKANNGSDNAMF
                     WNYTGSNGIELAADGNVRINAKGKQFTFANNGNLGLVASLDQSSVPQGTYHQVALNTG
                     TVGGKSYLRKFRGGNTDTIWHETVQGGLLRWATGNADEQEELSISTGYGVRARGEITS
                     LSANGLRVAYGNYGFFIRNDGGTTYFMLTASGDKFGSWNALRPMYINNASGAVTMGNG
                     LSLAGGLNVTSGNIRIPTSSTSWIDMRNNAALSNSSAVATSSASAIIRQEHADRHYFV
                     GGLGNSQFGFYMINKSRTANGTDANAYLQNDGTWVCGGNGSFNDVYIRSDRRSKRNIR
                     KIERALDKLEQIEGVLYEIQVCDRYEQSGGLIAQDVQNVQPELVTVDHNDQSGEPRLR
                     LNYNGVIGMLVEAVKELREEVRELKAKM"
     gene            44478..45260
                     /locus_tag="OO372_000048"
     CDS             44478..45260
                     /locus_tag="OO372_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="receptor-recognizing protein"
                     /protein_id="HCR6339673.1"
                     /translation="MPIVGVPGWIGSSAVSVTGQRWMSAARTAVQLPAAGSMSQMAGR
                     SKEVQYSIGANHNYNKDTLINYLKSQGATPVVVTITGDLVSSSSGVPCLDFPSSLTNS
                     YISLVINAGVTVYGRGGNGGSNAAGAAGGNAINNGIGTRLRITNNGAIAGGGGGGGGG
                     NRGRLVFGGGGGCPFGAGGSSSHMSSGATAGTISTPGKGSVGEGSLSAYTGGSGGNVG
                     AAGGRCNTQGNGTEYNGGAAGKAVTGNAPTWTKVGAIYGAHV"
     gene            45335..45658
                     /locus_tag="OO372_000049"
     CDS             45335..45658
                     /locus_tag="OO372_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339674.1"
                     /translation="MSNEMAGVTTEQVERIAAIVAREVVGKLGKELREEIGQEVNDQL
                     KTYFGDMTPAQHSIQHSNLDKLLNRLDSISSGFFGGIVSKITSFIITALLLGLAAYGV
                     KSGLQ"
     gene            45672..46364
                     /locus_tag="OO372_000050"
     CDS             45672..46364
                     /locus_tag="OO372_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339675.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLVANPDEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGTSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMV"
     gene            46366..46617
                     /locus_tag="OO372_000051"
     CDS             46366..46617
                     /locus_tag="OO372_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339676.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQNTVKVVNETSSKIRQKDASAVHRELYDKWVRH"
     gene            complement(46786..47307)
                     /locus_tag="OO372_000052"
     CDS             complement(46786..47307)
                     /locus_tag="OO372_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6339677.1"
                     /translation="MSNTTIEIFSGEKDYDLNGFDCGEESLNAFLVNHLKRQHEGKIL
                     RAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQKKVPYRNVPSVTLGRLALDKSL
                     QGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
                     FYPTKSIEKLFEE"
     gene            complement(47315..47584)
                     /locus_tag="OO372_000053"
     CDS             complement(47315..47584)
                     /locus_tag="OO372_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6339678.1"
                     /translation="MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERA
                     VKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKRAANRLKSME"
     gene            47904..48572
                     /locus_tag="OO372_000054"
     CDS             47904..48572
                     /locus_tag="OO372_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6339679.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEKE"
     gene            48578..48931
                     /locus_tag="OO372_000055"
     CDS             48578..48931
                     /locus_tag="OO372_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339680.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(48984..49751)
                     /locus_tag="OO372_000056"
     CDS             complement(48984..49751)
                     /locus_tag="OO372_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339681.1"
                     /translation="MDDLSVYARSLDKAKYYVYCLYDTEDKMKRPFYIGKGKSTRCLD
                     HIKYPDDSPKSMRIKELLANKKLGIDILRHGMDETTAKLVEATCIDLMGVGELTNKVR
                     GSGSMMGRISLDAYHHLVLQEETEIAPEHAGLAFLLNSTYKSGMSALALYEATRGVWA
                     KVPRDENLKYAYATYGGLIMEVYQIECWVKAGSQQYFTRDIALGPDTKRYEFVGRIAD
                     EHIRKLYVGKLIKKPPSYGSPFVKVGVVRTEHSVSAA"
     gene            50034..50774
                     /locus_tag="OO372_000057"
     CDS             50034..50774
                     /locus_tag="OO372_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339682.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEEK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILERLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            50818..52158
                     /locus_tag="OO372_000058"
     CDS             50818..52158
                     /locus_tag="OO372_000058"
                     /inference="COORDINATES: protein motif:HMM:NF015736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6339683.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            52306..53418
                     /locus_tag="OO372_000059"
     CDS             52306..53418
                     /locus_tag="OO372_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6339684.1"
                     /translation="MSELKELITELDFEQWLDTEGIVYRRGGVSARGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEEIVLASTVELEGPLALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGDATPEQIIKAYYRAKPYTKQLELQSKVLGIKAL"
     gene            53497..54273
                     /locus_tag="OO372_000060"
     CDS             53497..54273
                     /locus_tag="OO372_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339685.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVELHVWGESSCSRRSALVVFISKENPLSLKVQVGAWMDGKVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAWKVVRIA
                     NELPMIEQEAKSYATNHVTELLAPYRLESNQRKMVSWGSW"
     gene            54554..54811
                     /locus_tag="OO372_000061"
     CDS             54554..54811
                     /locus_tag="OO372_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339686.1"
                     /translation="MMVEEIRKGESLYFEDMTALIKEVEERNKQARTIKRKRDSVTIN
                     SMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEAKQQ"
     gene            54808..56130
                     /locus_tag="OO372_000062"
     CDS             54808..56130
                     /locus_tag="OO372_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HCR6339687.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALTNMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESVPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAIL
                     VALFKNDRSEDYAQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            56127..56342
                     /locus_tag="OO372_000063"
     CDS             56127..56342
                     /locus_tag="OO372_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339688.1"
                     /translation="MIVLSKREKETLHEISKWPEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKVLLQQLVESGVLK"
     gene            56339..56491
                     /locus_tag="OO372_000064"
     CDS             56339..56491
                     /locus_tag="OO372_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339689.1"
                     /translation="MIPYISLAFMGGFLIGFGICRDLIKQELKTKTLCIGKRVYRVVH
                     ETKVKK"
     gene            56488..56778
                     /locus_tag="OO372_000065"
     CDS             56488..56778
                     /locus_tag="OO372_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HCR6339690.1"
                     /translation="MSNLTSWEWWLATYFLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            57557..57631
                     /locus_tag="OO372_000066"
     tRNA            57557..57631
                     /locus_tag="OO372_000066"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57589..57591,aa:Asn,seq:gtt)
     gene            57634..57709
                     /locus_tag="OO372_000067"
     tRNA            57634..57709
                     /locus_tag="OO372_000067"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57667..57669,aa:Thr,seq:tgt)
     gene            58030..58116
                     /locus_tag="OO372_000068"
     tRNA            58030..58116
                     /locus_tag="OO372_000068"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58062..58064,aa:Ser,seq:tga)
     gene            58338..58688
                     /locus_tag="OO372_000069"
     CDS             58338..58688
                     /locus_tag="OO372_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339691.1"
                     /translation="MTSKDNTPLKVTPEHIEQLIQSEHYFTAYDASHGDNFISVYNSK
                     TDIDKGHESLKLLTFCVMVLKNGYTVTGKSACVRPEIFNFDVGRECARKDAIDQIWPL
                     EGYLLKQKWHEAKQ"
     gene            58685..58930
                     /locus_tag="OO372_000070"
     CDS             58685..58930
                     /locus_tag="OO372_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HCR6339692.1"
                     /translation="MTITIYGRDNCSYCKRAVELAKQLKGHGCGDYEYIDITTAGIDK
                     EKLSEIVGKPVETIPQVLIDGQPIGGYTELAAYVSTL"
     gene            59141..60244
                     /locus_tag="OO372_000071"
     CDS             59141..60244
                     /locus_tag="OO372_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6339693.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAVMISQVSNDMRFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSSLMRTCIEELFEYGKDSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELE
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRVNFS"
     gene            complement(60239..60625)
                     /locus_tag="OO372_000072"
     CDS             complement(60239..60625)
                     /locus_tag="OO372_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6339694.1"
                     /translation="MSDNKIEFIESRYAAFIAGLIESSPMSQAQIAKTIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPNRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            60877..61089
                     /locus_tag="OO372_000073"
     CDS             60877..61089
                     /locus_tag="OO372_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339695.1"
                     /translation="MKTTISSLIALEIGHVQKLADECVADILTDLPNEQIQVGVNDTT
                     GFIFELNNKRFTLLNTGSGSLAVRIC"
     gene            61188..63296
                     /locus_tag="OO372_000074"
     CDS             61188..63296
                     /locus_tag="OO372_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HCR6339696.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSWSLW
                     NALEDVFIERKMGQVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCGKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENNEDNEDHETPSSLDNHKSIKSES
                     HSKHKHDNNDSDDSDNPESSETIFDDTENDKAVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSMEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLTMNDDRVFRKKEESKAVNACVQQ
                     VIDLSGSMSGETIKLALASAYTIADALDRINVPNIITGFTTFGSHMAAGELKAIKYEF
                     SRFESLMLPIIKNWNEKANSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRLED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVTKQIETQTDIDLMAIGIMTDAPERFYANHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            63392..64627
                     /locus_tag="OO372_000075"
     CDS             63392..64627
                     /locus_tag="OO372_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6339697.1"
                     /translation="MTTTALQNDKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKEAEINMLINA
                     GIVDEYAEKMVKFAGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            64808..68326
                     /gene="dnaE"
                     /locus_tag="OO372_000076"
     CDS             64808..68326
                     /gene="dnaE"
                     /locus_tag="OO372_000076"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6339698.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRAKLAKESMRCMESLERGRNYSFTALIK
                     NEQGYRDICELMTAANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNAEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDAISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMTPESLHPVLD
                     ELTAETNGVILYQEQTMNATRLLAGFTMAEADGVRKAIGKKDMEKMKSMGEKFIVQAQ
                     AGWIDVELEDGTTQRIHRAEHFKCEDGTLKTVEEALEHGATLPINAVRVTASHPGLSE
                     MKAKEIWTAFEKNGAYQFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQ
                     GLVKDALTYGIRVLPPDVNVSSNRIEIRTLEDGSQALYAPFSAVKGCSENGCQAIMRA
                     REKVGGKFESVAQFDEAVEKRACNSRVRESLHKVGAFASIEPGSLPATDPERLRDQAE
                     LMGNLIIDAVKASRPFEMNPKRSAEINVLMTRMAAEMGLGEELIRPTIGIKPKIMIIL
                     DNANGNDARTGYFMENGYDDFKAKLLTVGDLRMGDLYVTGVCKKVKDKEKDYTKDEIG
                     QFTDFMREEINLVRPTYILTCGSRSTALFNNKSKPSDLIGRKEYFPELDATVFYGFNP
                     NILYFRPEEGERLEAILADIAETINK"
     gene            complement(68630..68845)
                     /gene="cspG"
                     /locus_tag="OO372_000077"
     CDS             complement(68630..68845)
                     /gene="cspG"
                     /locus_tag="OO372_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6339699.1"
                     /translation="MSNKMTGLVKWFNSDKGFGFISPADGSKDVFVHFSAIQSNNFRT
                     LEEGQQVEFSIENGAKGPAAANVVALS"
     gene            complement(69175..69738)
                     /locus_tag="OO372_000078"
     CDS             complement(69175..69738)
                     /locus_tag="OO372_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6339700.1"
                     /translation="MALYNFTLTLSGVSYETEGLEDALYQNGCDDALICAYGNSVYVE
                     FDREAQSLDAAIASAVDNIESAGIGAIVESVDSALVGLSDIAELTDLSRQAVALLKDG
                     KRGSGDFPCPVQRIKGQSPLWDWADVAEWLMNNGRLKEGDQIVQNARTLSKWNLALRN
                     SVSKDFNEIEQIAEKLIKRRKDIAKCA"
     gene            complement(69799..70023)
                     /locus_tag="OO372_000079"
     CDS             complement(69799..70023)
                     /locus_tag="OO372_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339701.1"
                     /translation="MKQHPNKHIQAAIDYALSKGWIWVAAGGAAHCFCRLRCGNPEGE
                     HKTHQMSVWSTPGNPENHAKQIKRMVDRCK"
     gene            70278..70709
                     /locus_tag="OO372_000080"
     CDS             70278..70709
                     /locus_tag="OO372_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339702.1"
                     /translation="MNTDIFSKIMADLEFDRDNLEEVWRKQPRLLMEYGSKLAQADRD
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDEARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            70827..71855
                     /locus_tag="OO372_000081"
     CDS             70827..71855
                     /locus_tag="OO372_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR6339703.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASSAAVAVSVKESEAVAATSVATASATEDEGLDD
                     LLRELDSL"
     gene            71916..72860
                     /locus_tag="OO372_000082"
     CDS             71916..72860
                     /locus_tag="OO372_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR6339704.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFAIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVSRIAPQPTVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            72860..73126
                     /locus_tag="OO372_000083"
     CDS             72860..73126
                     /locus_tag="OO372_000083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339705.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            73129..75468
                     /locus_tag="OO372_000084"
     CDS             73129..75468
                     /locus_tag="OO372_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6339706.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKCVTADTMLLTERGMVTVKELFEIEGYKATCTTRDVEHN
                     VGLINENGVIEKTSHLTWNNRRKFKRIKLASGGYIEATFRHPIRVVDDLGNVVWRHAE
                     KISVGDTIPSMVGTHQFGDQHLDANIAKLMGYLIADGYVASENSVNFSNTDPFIKDEY
                     YRLISLVSDKMPVTRKHNGSEDHVLFSKEVRSLLFKEYGLEYEKAAGKQVPLSVRRAN
                     SEAQIAFLRGYFELECHVNDGRCIEVVSASGLLLQQIRLMLLNLGITSTISEKHVAGY
                     ENTYYRLSFSGSNYDLFLSTIGFESPARLSVATKRDIGFDRTYSGYVPHISGLVKSLY
                     ESLTKTTREDYALVDHVIGRGDRVGIDKLREIYVSFIGRKNRFNEHLFAQLAAVIGSN
                     LFYDEVVAIEEGEAPTFDVAMPETHSFWSNGIISHNTFLATAAMISAQKQDGLAVFLD
                     HENSFDVGLAVANGLNADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPI
                     CIVADSLASMVPNSKAEKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARK
                     YNACIIFLNQVRTKIGVMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVG
                     AECIKNKVAPPYGKCTWKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKY
                     TKSQIVEMYREKPLAEIIAALQTIDDRREKTSPTEELED"
     gene            75562..75762
                     /locus_tag="OO372_000085"
     CDS             75562..75762
                     /locus_tag="OO372_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339707.1"
                     /translation="MIKLYLMAVVTGLSVVFIYCLLVPSLISTKSDIAVMLGIIVAFG
                     APVIGFIAGRKFLKSLINSKEK"
     gene            75766..76596
                     /locus_tag="OO372_000086"
     CDS             75766..76596
                     /locus_tag="OO372_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6339708.1"
                     /translation="MKKGLLAVIVAAVCTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            76688..77113
                     /locus_tag="OO372_000087"
     CDS             76688..77113
                     /locus_tag="OO372_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339709.1"
                     /translation="MTAIKKLYDAANVALDVIDDEVAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPDETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            77169..77444
                     /locus_tag="OO372_000088"
     CDS             77169..77444
                     /locus_tag="OO372_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339710.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKADGLNDEETDALIQRILVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISDEDAAHLERILSISTFE"
     gene            77660..77995
                     /locus_tag="OO372_000089"
     CDS             77660..77995
                     /locus_tag="OO372_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339711.1"
                     /translation="MKAIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            77995..78207
                     /locus_tag="OO372_000090"
     CDS             77995..78207
                     /locus_tag="OO372_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339712.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(78697..79752)
                     /gene="repA"
                     /locus_tag="OO372_000091"
     CDS             complement(78697..79752)
                     /gene="repA"
                     /locus_tag="OO372_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HCR6339713.1"
                     /translation="MSENGNKNIAIVEAFSETDKKTGEVVTLVPNTNNTVQPVALMRL
                     GLFVPTLKSTSRGRKGQMVSMDASAELKQLSLAKAEGYEDIRISGLRLDMDNDFKTWV
                     GIIHAFAKHKVVGDTVTLPFVEFVRLCGIPTARSSAKLRKRLDSSLSRIATNTISFRS
                     KGSDEFYVTHLVQTAKYSVKHDTVELKADPKIFELYQFDKKVLLQLRAINELSRKESA
                     QALYTFIESLPPDPAPISLARLRARLNLTSRTITQNATVRKAMEQLREIGYLDYTEVK
                     RGNSVYFVIHYRRPKLRQAQISTKIDNDETEYSLPDENQDDIIDVVPDEKEGKMVMLS
                     KEELALLEELRKAKTRK"
     gene            80496..81140
                     /locus_tag="OO372_000092"
     CDS             80496..81140
                     /locus_tag="OO372_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339714.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            81395..82480
                     /locus_tag="OO372_000093"
     CDS             81395..82480
                     /locus_tag="OO372_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCR6339715.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            82710..84626
                     /locus_tag="OO372_000094"
     CDS             82710..84626
                     /locus_tag="OO372_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6339716.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIESERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKESFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            84616..85359
                     /locus_tag="OO372_000095"
     CDS             84616..85359
                     /locus_tag="OO372_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339717.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NYPQPTLQTAIVANSLEARLLENPIWGTW"
     gene            85369..85938
                     /locus_tag="OO372_000096"
     CDS             85369..85938
                     /locus_tag="OO372_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339718.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            86012..88327
                     /gene="nrdA"
                     /locus_tag="OO372_000097"
     CDS             86012..88327
                     /gene="nrdA"
                     /locus_tag="OO372_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6339719.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGITTSQLHEALVKSAADLISPDAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQDLFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEAQNPLTQAVAVEPEDECDGACKI"
     gene            88434..89576
                     /gene="nrdB"
                     /locus_tag="OO372_000098"
     CDS             88434..89576
                     /gene="nrdB"
                     /locus_tag="OO372_000098"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6339720.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            89654..90523
                     /locus_tag="OO372_000099"
     CDS             89654..90523
                     /locus_tag="OO372_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HCR6339721.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVSIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLEKKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            90694..91797
                     /locus_tag="OO372_000100"
     CDS             90694..91797
                     /locus_tag="OO372_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6339722.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGIQHNRHGHESIYTQMLCLLERDGGITENMLDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVEELMKREPPKESDPVVI
                     FPHEWAELDDFKWEGVQVCGYSPLPWIKVPVAV"
     gene            91811..92212
                     /locus_tag="OO372_000101"
     CDS             91811..92212
                     /locus_tag="OO372_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339723.1"
                     /translation="MYVLCEIEGVLANASHRKSVSDADAGQLIAGDELIFPTSRMLRG
                     FARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLAEHKG
                     DLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            92209..92685
                     /locus_tag="OO372_000102"
     CDS             92209..92685
                     /locus_tag="OO372_000102"
                     /inference="COORDINATES: protein motif:HMM:NF012413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR6339724.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETAYTQRASEEEPGFSQIVYRRS"
     gene            92685..93329
                     /locus_tag="OO372_000103"
     CDS             92685..93329
                     /locus_tag="OO372_000103"
                     /inference="COORDINATES: protein motif:HMM:NF024911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6339725.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            93391..93810
                     /locus_tag="OO372_000104"
     CDS             93391..93810
                     /locus_tag="OO372_000104"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339726.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRTVF
                     EIAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            93820..94374
                     /locus_tag="OO372_000105"
     CDS             93820..94374
                     /locus_tag="OO372_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCR6339727.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQTMVDSFAM
                     RFNPRRSIDPKAQAVHGISLEDLAAEPLLADHASSVASYLSSSDIWIAHNGEAFDIPF
                     IRHELKSYGFSLPDIPLIDTLFSLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYDT
                     NLMMQCFFKARNKYGFFKLPSEIV"
     gene            94535..95344
                     /locus_tag="OO372_000106"
     CDS             94535..95344
                     /locus_tag="OO372_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339728.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESVESAENEPEQL
                     GLIEVEECVDVNDELKVQQSNDSNTNKKARTARGPRFTLSDKDDSFFNKAGLETDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            95528..96121
                     /locus_tag="OO372_000107"
     CDS             95528..96121
                     /locus_tag="OO372_000107"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HCR6339729.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRQSKKAA"
     gene            96308..96538
                     /locus_tag="OO372_000108"
     CDS             96308..96538
                     /locus_tag="OO372_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339730.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRENNQSQ"
     gene            97122..97715
                     /gene="rnhA"
                     /locus_tag="OO372_000109"
     CDS             97122..97715
                     /gene="rnhA"
                     /locus_tag="OO372_000109"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6339731.1"
                     /translation="MTTKTPARSQAKTRKKAKNKNSPRTNPTTPVVEFNPQLKTVKIF
                     SDGSCLKNPGGPGGYGIVLQYRGEEREFSDGFHSTTNNRMEMMGALIGLERLKYPCNV
                     ILYSDSQYLKNGMTQWMKWWKRNGWMTSDKKPVKNVDLWKRLDEAASRHNVRWKWVKG
                     HAGHRENEICDRLAKIAAFSAADTPHKKDIGFIYNKW"
     gene            97861..98355
                     /locus_tag="OO372_000110"
     CDS             97861..98355
                     /locus_tag="OO372_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339732.1"
                     /translation="MARQTYFTSATKRPRSLRQILAELFSGRVLSRLDELETTVRLLN
                     ERLDKQASVVANVGAIVTSGSSHQAKNARPLVKEKNNKDSSNGKFSKKEAETNGLRSH
                     YSFTGDGSRSSRPEPFDAGFIHHHTSVDDNYHHSSRASCHSGWDDGGCDTSSSSSYSG
                     SCCD"
     gene            98365..98553
                     /locus_tag="OO372_000111"
     CDS             98365..98553
                     /locus_tag="OO372_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339733.1"
                     /translation="MNWFSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFVFPVGSVLGYF"
     gene            98647..99072
                     /locus_tag="OO372_000112"
     CDS             98647..99072
                     /locus_tag="OO372_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite/colicin resistance protein"
                     /protein_id="HCR6339734.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGAYMISYADGTCDA
                     KEIAILEKTIAALPAFSPFAGEIAQMSANIRARYEASPRSANAQAIRELSDIAGTPEA
                     VDVLCLCLDIADQDGIGEQEEQALKKIAQALQLSLDAYL"
     gene            99072..99230
                     /locus_tag="OO372_000113"
     CDS             99072..99230
                     /locus_tag="OO372_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6339735.1"
                     /translation="MLERFRLVIVIALLVMAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            99370..99939
                     /locus_tag="OO372_000114"
     CDS             99370..99939
                     /locus_tag="OO372_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339736.1"
                     /translation="MCKKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKERADKLQATLDMVSETQRTDDTNKSEAI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMAVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKAFQSSN"
     gene            100279..100893
                     /locus_tag="OO372_000115"
     CDS             100279..100893
                     /locus_tag="OO372_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339737.1"
                     /translation="MFGMSITQSDGSLWMSPEFTPQNLINKGTMSTSKGSVFQTSIPS
                     NKSCFFFIKSSNKANMMFIHEHSNGYNALRLHQVNGSPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGAMVYHGEMMPLDAKLITISDPQFTIDMGYPCAVMPAMVGVYNYRRTDYDRP
                     VYVTMTGATGNQVYNGQWYSGNVTWDIKKIYTNKILVINTSKYD"
     gene            100997..102682
                     /locus_tag="OO372_000116"
     CDS             100997..102682
                     /locus_tag="OO372_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR6339738.1"
                     /translation="MNTALSIIDDANSNTAIDYRQEMNVIHEIVAECEKEIAFMYQVH
                     DFVYGDERHNMINRLLRLNHRPDEDRSRLNRGWLDKVDLEWVKQNIWAEYWRKVTDMT
                     NVLLIMPASRRDEWREQFIEGKQEVIKTDRTGYQMKVKEFVGVPEFKAETVIPTMLNL
                     LNDRHKYLSERVYGLFKALSPAHKTNKTNGFSERLIIADCISDFWRDSVSVNYRKEDY
                     IDDLRVLLHFFAHKEFITINRTAEVLSAAYRANDCQTGDWMNVDGNLMRVKMFKNGNV
                     HFEIHPDVAWKLNEVLAYSMPAAIPAPCRTAPKTRAPKQFGLIQKTISVPVRTALRDG
                     RLSKDKGVWYFSDSALQKSQVEELERTLSFIGGVQEKKHWQFPYDIGHTLNTIVATGL
                     IPDTKSHQFYPTPRLIAEYVARATELKPGEKLLEPQAGRGDLLAYINADLEDVTCIEI
                     APLFADILRGKGYTNTICCDFIKWSEDNAGYQFDKIVMNPPYSLGRHREHTLAALGHL
                     KVGGRLVAVLPGTAPILDWMTMDNYVYARGKSFTNEFEDTGITVSVYVFKRVK"
     gene            102744..103448
                     /locus_tag="OO372_000117"
     CDS             102744..103448
                     /locus_tag="OO372_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339739.1"
                     /translation="MNNLQLEHFNVTGHSDFPFKFTLKGYAEDAVGQIIIDKGIVKFE
                     GNFDESAKTFIDFVAKRWSEQWKDLEKRASEFDRFMDAMDTAKEALDAGTPLDLESLF
                     NGEVASAMFATMFAGEFVRSGAKNYLELDYNVPAIGDFVVTIQRKEGKTPGERVAELE
                     TVVDQRNGECDRLINELHALREERICAGSNTRNAADIYFQLVEECQIPPGGSLVDYVR
                     HLMTEVNSSHKDGEVR"
     gene            103448..103828
                     /locus_tag="OO372_000118"
     CDS             103448..103828
                     /locus_tag="OO372_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339740.1"
                     /translation="MFGIDAQRVAAFAKSPLDNPLSRSEQMELARLFLHIQKQADIFN
                     NMPNQPILDGHIQMVINSHEKGWAAIVPCTITYRLAKEVQEFRKASVESESTKAAINT
                     LIRMGFTWTGGAYWQAPHPILSGN"
     gene            103966..104517
                     /locus_tag="OO372_000119"
     CDS             103966..104517
                     /locus_tag="OO372_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339741.1"
                     /translation="MSNKIENPVVLIHKRENHDSYAVAITNGSHDFYDGLLMASVSPD
                     EADNSFAVFAMVGYYMAAEIEKLRAQRDALAAENVALRSKAAELAHEASKIYSAYNAT
                     ITEPDGDFMDMQTLHEMQCIETPATDAFLAEVRAQGVDMARNAMIDFVDGEVGPNKNV
                     PGLIRGAEICVSIAEQLRKGGNQ"
     gene            104514..105320
                     /locus_tag="OO372_000120"
     CDS             104514..105320
                     /locus_tag="OO372_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6339742.1"
                     /translation="MSKIDYQALREKAEKATCGEWALEYGDGRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLVLLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELEAAEKRIAELESLMEPKLPQPAVVDNDKFRADFERWMVEDEKC
                     IVGSSDPYPAGIESRNWRAWNACRAAMLQSQDSGIKDSWISCSERMPDTKTAVLVAKE
                     FDRKGDWRMKWATYIPGHPDANDGWIIPGASWKPSHWMQLPEPPQESQSE"
     gene            105534..106385
                     /locus_tag="OO372_000121"
     CDS             105534..106385
                     /locus_tag="OO372_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339743.1"
                     /translation="MHKSVAGEFQKEVDNTTVLLDDILSILALLEAGDWSEHCTKTEL
                     GGRLEREITRLIGDAQEATVTSYELIAEAWRLMDGQDPKTSDWHSKASKYLNSNRVEK
                     VDDVKPKPVDHGFRDNCECSSCQTTARICSELTDKSSLIYEVNVGGNTWVECTRAAYV
                     RAKDKGELTRVVAHHPNNELKDHQIRELVNELRDIAVQYHGTQQLREKIARAVNNSLK
                     GGQSQPKRIRITHIGENGTEIPQFSVGDIFDVHGEEFGEPHITMPSGHVYWLKAMEQY
                     GDKWEVL"
     gene            106399..106923
                     /locus_tag="OO372_000122"
     CDS             106399..106923
                     /locus_tag="OO372_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR6339744.1"
                     /translation="MIKFGKLEAHLLTRNYRHDIHPYRQWIDESEGVVTFPLYRFDGM
                     LAGYQTYRPGAPKQHSNPKMARYFTRANGRQLLWGTYLPLKDGPIWITESIFKSAAVH
                     NAGGNSWALLGSTFSAGLRRQLAMLPYDFRVIGDNDAAGESLVKSFGKGFVAPDLDEL
                     QPNEVSHLIFSHSQ"
     gene            107096..107485
                     /locus_tag="OO372_000123"
     CDS             107096..107485
                     /locus_tag="OO372_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HCR6339745.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKYPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(107523..108623)
                     /gene="apbC"
                     /locus_tag="OO372_000124"
     CDS             complement(107523..108623)
                     /gene="apbC"
                     /locus_tag="OO372_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6339746.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            108781..>109775
                     /locus_tag="OO372_000125"
     CDS             108781..>109775
                     /locus_tag="OO372_000125"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6339747.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGA"
BASE COUNT        30822 a        24108 c        26768 g        28077 t
ORIGIN      
        1 gtgaatgccg atatcgtgaa caaagtggtt aacctggcct ctcgtaatgc aggctttatc
       61 aacaagcgtt ttgacggcgt gctggcaggc gaactggctg acccgcaact gtacaaaacc
      121 tttactgatg ccgctgaagt gattggcgaa gcatgggaaa gccgcgaatt tggtaaagct
      181 atccgtgaaa tcatggcatt ggctgacctg gctaaccgct atgtcgatga acaggctccg
      241 tgggtggtgg cgaagcagga aggacgcgat gccgatctgc aggcgatttg ctctatgggc
      301 attaacctgt tccgcgtgct gatgacgtac ctgaaaccgg tactgccaaa actgactgaa
      361 cgtgcagagg cattcctcaa tacagaacta acctgggatg gcatccagca accgctgctg
      421 ggccataaag tgaatccgtt caaggcactg tataaccgca tcgatatgaa gcaggttgaa
      481 gcactggtgg aagcatccaa agaagaagtg aaagccacag ccgcaccggt aactggccca
      541 ctggctgacg acccgattca ggaaactatc acctttgacg acttcgccaa agttgacctg
      601 cgcgtggcgc tgattgaaaa cgcagagttt gtagaaggtt ctgacaaact gcttcgcctg
      661 acgctggatc tcggtggtga aaagcgtaat gtcttctcag gcatccgttc cgcttatcca
      721 gatccacagg cactgattgg tcgtcacacc attatggtgg ctaacctggc accgcgtaaa
      781 atgcgcttcg gtatctccga aggcatggtg atggctgctg gccccggcgg gaaagatatc
      841 ttcctgttaa gcccggatgc tggtgctaaa ccaggccacc aggttaagta attaaacaac
      901 ctctgttcgc aatgcccggc aacacgctgg gcattttcat atagacacaa tcccttctga
      961 tttacgcata ccctaaaaca atttgttttc taccttttgt taattgagat aataagccac
     1021 ataagaaaac aaattgtttc ggagtggttg aaatgttgaa cattacccaa gcctaccagg
     1081 tggcattgat cagttcatct catacatcag aggaagatgc tcgccgattc atgatcgcaa
     1141 gcaatgagct ggattgggtt tcgcaaattg acggcggttg tattgtccac gccgggttac
     1201 aggatgatgt ctggaaagac gacttgcgtc gatatggtat atccgaaggc gcgattgcta
     1261 acattcagaa agtacttgat gtaggctttg actctgtaca ttttgattgt ggtgcaccgg
     1321 tcgttgaagg gcttgagtgc tggaactggt aacagatatt aaagcattgg tagtttgttt
     1381 ttaggattat caaagaaatg aaaaagttga aaagtttaga aatccctgga actggttata
     1441 aattctttat ggacaacgcg gagtgtgcac ctaacgcgtt ggaaaaaata caaagtctta
     1501 tcaacagcct tactgaagaa gcgaaagaac tgaagagcga ggcgacaaag gacaatacag
     1561 ctaaaatcca atgcttaaat ctcgtttctt attgtttata tcaagtgtcc aagacactga
     1621 ttaaaggcaa tatctattac gacgatgaag acacctcgac acctgaagaa aaagatagcg
     1681 atgaagaact ctatgaagat atctctacta gtgcgggtca cgctgccttc gatgctatgg
     1741 gtaaatcgtc aggcatcaga tcgtttgttg aacagaacaa ttcgacaata atgattgagt
     1801 actgtgaaca aacaagctcc aaggccatac atgttcttca cgatgttgct gcaggggttc
     1861 gctgtgaggc tgaagcggtc agaaaagatg tatttggtgc agacagtaat accgtgaaag
     1921 catttatcaa cagtgttctg ctaaattctt actcaaacct tatagtcaat gcggcactat
     1981 gcgcaaattc tggtgaagaa cacattgtca taaccgaaac caaacatgag catcaacagt
     2041 caccaactac agaagaggta actgcattgg ctatcaaaca ggcgagtgaa attagtgatg
     2101 aaattctctc tctgctaaac aaaactaagt aaaacattga taatggcggt cacaatttga
     2161 ttcttttcta taagaatccg ttgatcgcca ctactcccct tccatgcctg caatcacatc
     2221 cttaaatatc aataggttgc agttgtttat acaatcaaga aaacaactaa ataacagatc
     2281 aacgaaacat caacgctttc acccgatttg gtctaaaaaa ttgacgtttc cacacatcac
     2341 agcttatacc cgtaattcta cgcagcagac agcgccacag cgtccaattt tcaccacaga
     2401 cgacccaaca cactaccaac aaaaacacac gctcaaaatc gctcccgttg cgttacacaa
     2461 ccatataaac aacttatttt caggtataac aaaacaacca aataacacat atgccattac
     2521 ccaaaacaac caacctcttt atggcaggct accgaaaaga cccacctctt cttcccaggc
     2581 taccggacaa ccaacctaac tttcacaggc aatccgaaaa cattcatacg cgcaataccc
     2641 acgatacgcc attacccaaa cagcgaagaa acaccagaat cactgaaacc ccaacgaagc
     2701 ctgtaaccgc aaggtttctc tcaatcccaa aagacacgaa acataacaat cacgggaaac
     2761 gccattatcc aatacacgaa taactacaaa agcctttcac acactccaga caaacaactc
     2821 aaacacaata acgaaagcaa tcgcccacct gccattactc catacacgga gaagaacaat
     2881 caccaaaacg acgagaacac cccatagaag aaccaccgca aaacgaacga aaccacatac
     2941 accgacagag agaacaacac acccaattca ccaacagaat tagtcgccgt atagaacgtt
     3001 ctggaaggag tgagtattaa cgtccataga gaggttgaga cagataaaac acgaataaaa
     3061 cacaacatgt atagcgaaga agcaaggtat gtataagtgg ggagggaagg aggggtgtcg
     3121 cctccttttt cgtatttatt caactcctga ttttatcccc cgtcacaccc ctacggtcag
     3181 ccttcggtcc atacagggaa aaggctgcat cccgctacag aaacggctgg gttgccttcg
     3241 gggaacggct ggaggttttc agggaaacgg tcgagttgcc tgtgtggatt tcgggaaaag
     3301 gctcgatgcc ggttcaggaa cggcggcgta ccggtatagc gtgagggaga gttgcggcta
     3361 cccacctgta atgtgcggga agaagagagg cgctcacgtg caggcgaact tacttaccgc
     3421 tggattccct ctgaatgccc agctacagcg taattgtgcc agacagccaa cgggtcacaa
     3481 tcgatttcag taggttttcg gggagaggat tattctgccc accagccacc ttcggccact
     3541 aaatcagaat aatgtaaaaa gtgtactcga tactgaacct gaaaactcac tgccaaaacc
     3601 acattcaccg gaataaaatc ctttttcgcc tgtgcgttat tgcgataata acaccatcaa
     3661 gaaaacatgt tgtttaagga ttgctttatg tttgcaaata tcgacatcaa ccaaattaag
     3721 aaattaactc aaaaagagtt tgatcagttt tatgagttag aaggttggtc ttccactctg
     3781 atcaattcgc ggtgggtgct tgagctgatg actcgtgatg acgcacctgc tttgatgatt
     3841 tgcgacatgg gtgaagatgc tgactttatg gatatgagcg aattttgtgt ggacacatac
     3901 aaccgcagcc agaagtacta cttcacatgc gatagcgaaa atgacgtgat ttctaaggtc
     3961 tatcttcacc ttgtccagca ttgggacgtt caggagtttc ttgaagtatt cgagtaagcc
     4021 taaccaaagc cagcaatgct ggcttaactt aatatgtagc cgcagggaag aacctgcata
     4081 tcgcccatgc acctgatcat tatgctttgt agccgttttc ttactggtat tatttacgcc
     4141 attgagaaaa caagttgttt acggagttgt gatgaaaaaa tcattggttc ttggtctgga
     4201 caaagaccag aagagaaaag agaagcctgc gctggttgct caattaactt tgctggacat
     4261 cgttgccaat ggaacctcta ttcgtctgtt ccgtgaaaca gcggtgtctt tcgataaaaa
     4321 cacctttact cgttatgtaa tgaatgttcg tcgccagcgt ggaaaaggct ggatggcatt
     4381 tcaaagaatg tggccggaac atcagctcga actggctttg atggaagtaa accgcgtggc
     4441 ccagcaagaa attcagagag catcagtgat ggcaatagcc tgataatgtg caagtggtaa
     4501 ttagtcgacc gtacgacagc cccgccatcc ttacggggct tttttgtatt gtaagtacat
     4561 acttacgatc atagaatata aaaataacca aaacactacg gagagtgaaa tgaccgttaa
     4621 acgcgaaaaa ctgacagttg atgtttacta tgcctctgaa accgccgaag gtaagaatgt
     4681 ggcaaaaatc accgttgtta cgtacaacac cgaaactggc gccgaagtcc aggccagtac
     4741 gatcgtgcgt aaaggtgatg cctccggcgg cgagtacgag actcaatacc agtctatttt
     4801 cgatgcaact gacccgctgc tgctgaaaat cgagaactac ttccgccagg ttgatgaaga
     4861 ggtgtttgaa ccaatgatga atatggttaa caccgtattc gcctccaacc tgaataccaa
     4921 cactacctgg attggtcagt acggcctgcg cattacctct ggcattcctg ccgccacctt
     4981 aatccctgaa agcgtattcg cttaatcctc tttaaatggc gcgtaaaccg cgccatttta
     5041 ttaagcccga taacaatttg ttttctgcct tatctgattt gtgaaaatga tttcactgaa
     5101 gcaacttaat aaggaaacca tcatgggact tgatatctat atcgagacac agcctaaaaa
     5161 cgatctgaat aacgaggcat ccagaaagca ggttgcttac ttccgtaagt tcaatgcgct
     5221 cgttgggtgg atggagcgca acgtaggtga agtcgaaaat tgtgagcttt tagaattaac
     5281 gatgaatgac atttgttttc tgaaggctca tttgatgcac ataaacgaaa gtaattgcga
     5341 agagtacttg cctacccggg aaggtttttt cttcggcagt caggagtacg atgaaggtta
     5401 ctggcatgat gtgggggagt tgaaagagct tgtggaagat ctgattaaga accacgactt
     5461 tcacaataac agactgacct tctgcgcctg gtggtaaata tgggcgattt caagaaacgc
     5521 ctgaaggaaa gagccgagat ggtcagaaag caaaacgcct cttctgtcat cagatacgca
     5581 aggcagttta gtcgcaacaa caaatcagtt gaggaaaaga tccttagcgt aatcgggcga
     5641 taattattaa ggccatcagc attggtggcc ttaaatgact tttaataacc gaaggttagt
     5701 ttgaaacctg gctatcaact tccagaacct ttcttgtgta ttcagcctgt attaacttct
     5761 tttatgcctt atctacactg cgataattac accaacaaga aaacaatatg tttacgcagt
     5821 gaggatatgc tcatgactga tttcaccatt acccctaaag cacaaaatgt attccttgaa
     5881 tcatggctgg acttgccgga aacagaacag caagaaatgg atcatgtgga atatgacgag
     5941 caggtaagca cgcgattctt ccactttgag ggctgcgttt acgacattgc cgacttcatg
     6001 cgagatgacc gcttccctga atggcacgca agctatccac taaacgcctt cgccatgctg
     6061 atgatccgtg tggatgattc aggggatacc atcgacatcg gtctgctcca ctaaagatta
     6121 aggccaccaa tgctggtggc cttaaatgac catcctgttt cccgcaggct aaaaacacca
     6181 acctcttacc tccaggcaac cgacaaaccc acctgttctc gtccggctac cgcaactttc
     6241 cactttgacg ccttattcgt acaacgataa ttaacaccaa caagaaaaca atttgttatt
     6301 tacgataagg aaataatcat gaattttatc gctactgtaa acgcacccgc acatggcaat
     6361 atcgctgtaa cgttctctga cattgaaaaa cgagtacttg gtgcatggcg cgacaatgag
     6421 acggtagaac tgtcagcaca agaaaaatgc attattgcac gcgacatcat tggcaatcgt
     6481 cgttactcgc gggtatttga gaaagcgtat gtggtaaatt ctgggttcgg aacgttcgtc
     6541 tttccggtgc gctccgggcg attctgccag tccaagctga ttgagttcgc tacgcagatt
     6601 tctgtctgga ttaaaactca atcgtcgttc aaattttccg acgatgaagc agtatcgcag
     6661 gggatgcgga tcgccaacaa tgcgattaaa tgcaaaaaca ttacgtatac cgctggcgtt
     6721 gatacatgga aactgttttg cgctaacttt atgctgaatg tatacgcaag caaccgcatc
     6781 cacatccttg atggcgtgta actgagaaga gggccagaaa cggccctttc tctatagcca
     6841 ccagctgccg cagggaaatt ttcagaaacg gcgaggaacg cattcatgag ccgacgggaa
     6901 acggccagga ttttttcggg aaacggctgc attcgccttt atatagaaaa aacatcggga
     6961 agctggtgga atccaaccag cggttgtcgg acaggtgagc ggggaaaatt atgatgactt
     7021 tcgtcgcctg agacatccag atttctttcg tagcgtaatc acatacgtga ttaagtggtg
     7081 tgattatgtg aaaaatcacg cgcacataat acgcgagcgg atacggaaca aaacaaaatg
     7141 ccgatccgcg ccgacaatta aacgcggatc acatagcaag aataaaagcc aatgattaac
     7201 catacaccat aacgcaatat aacgcgattt aagcgcgtta atgcgctaag taatgggtat
     7261 gtactggtaa ggatataaaa gcgcgtctat ggcgttattt tggcgcttat ttttatgttg
     7321 ttggcttgag tcaaagacaa taaaaaagcg ccatcattgg cgcgttatgg tggaagtatt
     7381 ggaaacgaaa aaagcgccca tagtgggcgc tcgattttat ttatgtagac tgatttcaaa
     7441 tcccatttct acaaacgctt ttaacattaa aaatatatca gcgtcattca cgtctgcttt
     7501 ttttcgctcc tggtcgctca ataagtcaat tttgcgcgtc gtttcatcta taagctcgac
     7561 tgcgcgccca gcgtaaccag cgatcccagc gatgcgatta acaaaatatt cattgcgcac
     7621 gttaacgcca gcgataagaa taaacatgat taagctcctt aaaagcgccc atagtgggcg
     7681 caagattcca ttaattacgc tttgaaagca tcagccagat agttatagaa atcattttta
     7741 atgaagcgat attgctgagt accagctttc gcgctcccag ctcctttgac tttttcaaca
     7801 agtccaagac gttcgcaaag attgatcaac tggttggctt gagtatagcc agcgtccaat
     7861 ttaatttcgc acgctttttt cgcttcattc attaaatcga aaacagcgcc attggtgaac
     7921 gtgtcgatct cgtcgttaat catatcgatt aatgcgaata cacgagatcc ggacatatca
     7981 gcgacggaat aaacacactt tccagcttta atggatttaa ccagataaac cagtttttcc
     8041 agagaataac tattagtcat tgcatcacgg aaaaatactt ctggcgcttg tttgcttgct
     8101 ttgatcgcat agtagaaaac agagcacaat ttttcgtctt ctacagcgtt tacaacgttg
     8161 ttgatgaagt aagcaagttt ggtggtcgca gcttgcatat ttgctttgtc tgctttggtg
     8221 tgcgtaccat ttttataatg ttcattatat gtttgagttg ccagatctgc tttttcgcgc
     8281 agctcgtcgg atacaatggc agcagcttca atgatggatt ttttagaaat gataatgtta
     8341 gtcatgataa tatccttatg aaagtttaaa atttaactca agaaagaaag tgttttgcac
     8401 tcgttcctga tgcgttcaat tatcgacacg cgaaaaaaga ttgcaagtat ttttttaagt
     8461 ttttttgcag ggatgaaagt cctagaaata aaagcgagat cgtcgaaggt gttccctgaa
     8521 taaataatta attccggctt ataaccttat atatttatac ggggtagaat tgaaagagta
     8581 ttaaatgaat ggcgtgtaaa ataataaccg gacttagccg gttattaccc ttataggaat
     8641 ttacatcttt tttgcatagt tcctgatttc ttttattata tattcctgaa aacgctccga
     8701 gttttcgcat ttgtctttcg aaggctcatc aagtcccaac ttgcatcccg cagcgatcag
     8761 tccatcttga tatgcagcgc ctaaagccat gcttaggtct aacatgcaca aatcgtagtc
     8821 ttccgccttt gaacacaact ctgtagagac acgttgcact cgttcaatgc tattttcttg
     8881 agaaaatgcg gaagagctaa aaatgaaagt taagaatgtg aatgctgcta cagttttcat
     8941 accaatatcc taaaacggta aaattcggtc aacccaatcg aagaaaacaa gaatttttct
     9001 gccgtcttca tacatttatc ggactgagtc tacagcctta cgattggtgt caaataacgc
     9061 atcaacgaac tgcacagaag agttgttcga ttcacatggt tcataatttt ccacttgttc
     9121 acatagttga gcggtcttac caatgtttat ccctagtttt acagctgctg aaagtttaaa
     9181 catgcaaact ggtacattct gagacgttga gcatacgtca ttcatcagcg actgaataat
     9241 tgcgatatca tcctttgcat tggccgaaat tgagtaaact aaaaataaag atgtcaatag
     9301 tatcgatttc ataactcaaa aaggtaaaat tcgttcaacc caatcgaaga aaacaagtat
     9361 ttttctgccg tcttctaact taagagtgac ctggcaagca tcaacgcctc ttgatacccc
     9421 ttctatttca cgaccgtctg ccatgtagac ccttatagat tggttgtttt gataagcctt
     9481 gcgacatatc gcaaaaaagt cacggcgtga tggctgatta accatctgtt cattgttcag
     9541 tgttagtcga cctgtgaatg aagtgtcaac ctcttctatg cctgactcaa ttgtgctaat
     9601 gcgctcaaga gggagcctta cacgatttgt gttgctaaac ggatctggtg tgaggttcag
     9661 cttatttctt gcgctcaaca gcccgtagac atacatgcaa aacacttggc cgtcttcgag
     9721 agtgactctt acaggaaatt gagcactccg ccagaagagc aaggtgcgtt cagcatgttc
     9781 gtagtttcgc ggccatactt cagccggtat gccgtaggtg atgtccaatg ttctcattct
     9841 gatactctta gaggtgtttt cagcaatcca aatttaaatt ctttgttacc aacgaccaag
     9901 aaggggactt caacatagat ttttttatcg acggatttag agttcgctgt tttcttgatg
     9961 tcgcgaacga tacgttttgt tgtgttttca tcaataaaca ataccttccc actggtattc
    10021 ccttcagtct tagcataagt gacaataggt tcttcattgc caactctgat gttcacttgg
    10081 caacccgttg tagccatttc atcacaatag agtgtaccgt cttgaatttc taaaacaatt
    10141 ccagatggct tcatggcctc gttagagctt acattagcgt tttctggtac tgcggtcccg
    10201 ttaatttgca accgtaatct ttcttttcct gcatgaagta ttgtttcttc tgcgttatct
    10261 gagaatctat accagactgg ttgaatcaga cctcgtgatt tgtattttag tacgtcttca
    10321 gaagtataca tgctgtacgt tttttgcatg tcagagacga aatctgataa tgcgttggcg
    10381 gaaaacgccg ggaggcatat acaagcaagg agtaacgaaa gtttcatttt catatcatga
    10441 taatccaatt atttaacatc acactttcta agtttttcta tttgttctaa tttttcatga
    10501 tcatcattta gatttccttt gactatctcc gtttcaagtt tgctgattag catgttaagt
    10561 gcgttgtagt tattgcattt tactttgtag gcgtagtatg accttgcccc tggtttttgt
    10621 acacccatat cattaaagtt agagcttaaa gtataacctt caagttcaac accttttatt
    10681 gttggaagaa ttatatttaa tggttctaat ttaaatgggt agatataaat atgtgatatg
    10741 aagtactctt ctgaagagat tttcttcatg tcttttgtga ttgaaactga atatttacag
    10801 tcccgagcga tataaaattt tgtacatttg tacggcacta agttgaatag ataatatttg
    10861 tctttaaaaa gaacatcttt tccatgtgat attttatgtc gtaaagagtt aattattttg
    10921 tttatagaaa tactcatcta cagtatccga cattagttat caattgaaaa gcctacaaat
    10981 atcgttcagc caatcaagaa attttttcag ccggaggtgt gtacttcaaa aaatgtgatg
    11041 aatgtcacat gcaggtgctc tcatacacct gcatgtagac tattaatcat tcaataatct
    11101 atattttctt ttgattaaat cttcaccttt tttggtgaac acaaaaccaa attgcgattt
    11161 tttgtttaga cgaaggactc tgttatccaa cgcatcggtt gatggaatca atgtttgatc
    11221 atctatccaa ttaagcagcg taagaaagtt cagcagcctc aacgttgttg tgcgaagctc
    11281 tttcgatata ttttcaatgc tggcaattcg tctttctttt ttctcaggtg gtacagataa
    11341 ttgttcttga gatttcgtgg atagataatc aatttcgttt gctgcatcga atgatatgtg
    11401 aggcgtaatt ttgctaaaaa agcctttcgc tgactcactt gataataaac cagacctatt
    11461 catatcgttt gttattctgt acaggagtgg actgtgtttc atttcaggat aacgttgata
    11521 aaagttaagc aattcaaaag cagcattagc gccaacaaga aaatcagaag gatcgctgac
    11581 tttccagaaa gaatgatttt tcttagcgcc agtggtgtca acaaatgata attcttttaa
    11641 ccaactcatg atgtgctcct ttctaacaag tgagcacatc atagaattag ttgatagggg
    11701 gtaaatatga aatgttacgg caacctgctc agtatttcct ctagatcttt ttttgtcata
    11761 ttggagttct catagatgcg catgattttc tcacgcgtct tagcggaaac cccaacggca
    11821 gatgtgactt tgtcaaattc agccatagtc atcgtctcca gaatggcatt gataacttca
    11881 gccttagaca tcttgatttt tttctctttg agttttattt gaaattttcc aagtttttca
    11941 ttggccttat cagacaatgc cacttggcaa taagttgttt tcttttcgct cataactaat
    12001 ctcgtttcag aactccaaaa tcgaatgcgc catcaatagg caatacacct tccgcaaagc
    12061 caggtgtggt gtcgatgatg tgttttcgct cataagagtg agacaacagg tatttgttgc
    12121 taatgtcaat gaaatcagtg ataaaacaca cgtttgcctg attcttcttg gctcgtaagc
    12181 cacgaccgac acgctgcctc atttcaactt ctgctttccc accaccagca agaatgaccg
    12241 caccaacgct tggcacatca acaccaacat ccagaatagt cgagcctatt aaaacatcta
    12301 tttcaccaga cgctaaactg ttcagctttg cttgccttgt cgactggttc gattccccat
    12361 aaatgaagtc aactctaagg ccggactctt tcatcatttc catcaggatt tgcccgtggc
    12421 gtttaagacg aaccagagtc atacaattga gagaatgttg cttatagagc aatgcttcgc
    12481 gaacaatggc ctcattacgt cccaaattat acacgatccc caactgataa gccttttggt
    12541 aggctgtgct catacctact ctaaaattga ggtgtttgtt ggcaagttcc gccttgattc
    12601 tgacctcgtc tggcttgtag gcaactttat gataaaggaa gtacggtttt gccagaatgc
    12661 ctcgatcaat caggtacttt tctgtgactt tgatttcaat tcgcccagcc acagccatca
    12721 gacgcatgtt ggcttctgta gaatccttca tgaacggcgt ggccgtaagt gccagacgat
    12781 agtctgcgtt cacacataat ctggcaatgt cataaaaatt agaacctgaa gactcatgcg
    12841 cctcttccag aataagcaga gacacacttg aaaggaagcg tttcaccaag tcccgacgtt
    12901 tgaggtggta gctcttctta tctggagttg catcgcgtgg tggttcttca aggaaacttg
    12961 ccagagtttg aactgtagcg acgttgatat gtcgtgatac ctggaactca ccagagccaa
    13021 tgactccaac cttttggtct tttaaccacg gttcgccatt ttcggcgcgg tagtcgatgg
    13081 atctctggaa gttttcggcc atctgaaaca tcagaacaga gcgggttgtt aaaaataatg
    13141 tcattcgacc gatacgtgca gccgctttgc aggcaacatt agatttcccg ccaccagtag
    13201 cgatctgcgc aatcatcatt ccctctcgaa ccagtgtttc cacagtctga tcctgatacg
    13261 cataatcagg attgtatggg aatgggttaa ccgccggatt tggtttacca agcgccgggg
    13321 ctttgtcttt gcggatatga acacatttga tgcccgcctt gttcaagttc gccgctacag
    13381 gcttggcaaa gccagcaggg aacgagtttt tactccagtt gaacatcgtg cttgtgccct
    13441 tccagtcgcc agtctcgact tcgtagctca acatttgctg caccagttgc tttaccttgt
    13501 catctgcgcc agaaataagc gcatttactg cgttagatac aatccgaaca gtcataaacc
    13561 tctttcctta gtgccttttg tatgttattt ggctattata ataagtaagt gattacttag
    13621 tggattgtag caataaaatg gatgtaaaaa tcacgatttt gcaggttgat gttgccaacc
    13681 ttcgcccgaa tacctggaac accaattcgg ttggtgcgca gaattttgaa aaactgaaag
    13741 gttctatcga aaaattgggc ttttttaagc caattttggc tcgtgaactt gaagatggat
    13801 tttttgaaat cctcggcggc gaacatcgct ggcgtgctgc tattgagcaa ggaatttcaa
    13861 cggttccggt gctttctgtg ggcaaaatta gcgatgtcgt agctaaacag atgtcactgg
    13921 tggacaacga gcgatacggt gaagacgacc aaatcgcatt gcaacgcttc attgaagaaa
    13981 ttcagtcaga acttgactat caactgtctg aaatcgcccc gtatgacgac gaaatctcga
    14041 tggttttagc aaaagaggcg gcaatcgacc ttgaagcact ggaggcgttg tctcgtggta
    14101 gtgatgagcc tgtcgatacc gacaaacgag agaaaaccga acgtgtgggt gcggaacatc
    14161 agaccatgcg cttcaaagta actttcgatg cgtcagatcg cgttgcagaa accataaaaa
    14221 acatcatcaa agagcaggct attaacaccg gtaatgaaat ggagaacgct ggtgaggctc
    14281 tggtgtggct ggtcgactac tacaaggagc gtatgtaatg accaaaaagt ttgaaatcgt
    14341 atatcgcgac ccggcagatc ttattcccta tgagatgaat gccaaaaagc atgatgaaca
    14401 gcagatccga gatctagccg cagccattaa aaagcgcgga tttgaccagc caattacggt
    14461 cgataagaac gacgtaatta ttactggcca cggccgccgt gaggctgcaa tttttgctgg
    14521 acttgagcgc gtaccggtta ttgttcgcga tgatctcagt gatgacgagg ttcgtgcgaa
    14581 gcgccttgaa gataaccgac ttgccagcat tgattacgat gcaattaagc tacagaaaga
    14641 gcttgagtcg cttgttctgg acgatatcga ggttttcggc tttgaagagc gtgagttgaa
    14701 tgttctcgtt ggcagcatga cagaagaaat ggacaccgac tcgctagtta tcgatcttgg
    14761 cgaagaaact aaacgacaga aggatgaaca caccgagatc aatcgtgaag ttgcagcgga
    14821 agaagtacgt gttgtcgacg tattgggctt taaaacgctt cctgctggct ctgccattgt
    14881 tgttggtgat ttgcttgccc atatggaaga aacgacggga gaaagcgggg tagacgcatt
    14941 tgtggcatat gcggagaaga tctcttccgg ggagatggct gcatgagcaa atacatcatc
    15001 aacgtatcgt ttcagacacg cgtaaataaa accacgcgca cgttggaaat cgctgagtcg
    15061 ttcgggcttg gcctggacga aaaagagtgg acgctttacg acaatctgga gctggaagtg
    15121 aagcagggcg atgtggtgta catcaccggc caatccggtt ccggtaaatc cgttgtgctg
    15181 cgcgagttgc aacgacagat gaaggatgaa gggctgtctg tagcctccat cgatgatttt
    15241 actttcgata atgaggttaa cgtcatcgat cagttgggca aaacgaccag cgatgcgctt
    15301 gggttgttat ctatggctgg tctgaacgat gcatatctgt ttgttcgcaa gccttctgaa
    15361 atgtcagacg gtcagaaata tcgtctcaag attgccaaac tgattgagtc aggcgctaaa
    15421 gtgtgggctg ctgacgagtt cggtgctgtt ctagaccgtg taaccgctca ggttgtggca
    15481 tctaacctcc agcgtgccgc tcgaaaggtt ggtgcgacgg taatggtggc gacgactcac
    15541 gaagacctga agaacgcgct gcgcccggat atgcagatca ccaagcacta caaagaacgt
    15601 gtgaaggtgg aatatcacaa tggtagtcat gatgaggttc atttatgacg gacatcatca
    15661 ttaaacgcta ccgccctgaa gagtttccgc gtcatctgga ctttctggag cgaatgactg
    15721 tcacaaaggg aactgtagag gactggcacg ctcttaagtc gcttcactac aaaacagacg
    15781 gcaaaccttt cgcgccaact tactatcgct gcgaacttga tgaccgtctg gtgggcgtcg
    15841 tggttatggc ttacccgaaa ctactgctgg cacctcgcca ccgcatgttt cctaagttga
    15901 aaccaaccac taataccacc gtggctaacc agtactgggg tcggtacgtg aataacaact
    15961 ttgcggtgat cagtcgctca gttgtggata ctcagtatcg tggcgtaggc gtctcttatc
    16021 gaatgattaa cctggttagc aggatgcatg accggccaat cattgagatc cagtcctcga
    16081 tgagcaaata caatccattc gccatgaaag cagggtttaa gttcatccgc cctgagcgac
    16141 cgaagagcta tgaaagtgca ctgcgtgtat tccagcgcca tttccgttcc gacccgggtg
    16201 ataacgaggc gatcgtcaaa gagttgttcg caatgagcga gtctcgtcgt cgccgtgcac
    16261 tgcgtgatct ggtggcggac taccacaaga acagttccct ggcaaaagct gggcggaatc
    16321 gtggcacgac gattcaggac attgccgaca gtctggtgga cgaggccagc attgtgaagc
    16381 tgctcaagga cattcacaac ctgagcttta cgtctccgtt gtatggtgtg taccgaaacc
    16441 ctgactttgg ccgtcgactg cctgacacgc tgccactgct ggcattcgac aaacaacctt
    16501 tggataaacc gttagaaatt gctttaccgg cataaggatt tgccatgacg ttaaccgata
    16561 aacaaaagga catcatcaaa acgctcaatc tcggttatga gcgaggtcat ctacttgacc
    16621 tggacgaatt gcttgaagtt ttgccgtaca agacaaccaa gcaaagtatc cagttctcaa
    16681 ttcgcgctct gataaaaaag gggctggtgg agaaagggca tacgcgccaa cgcagtgaca
    16741 atcgctatca ccgccgaact cttgggttaa ccactttagg tcgagccaaa gcgaagttac
    16801 tggtgatgta atcggtctgg gagcttattt aaagacctgc atctgtatat ataaataata
    16861 agtaacttat taaatatata cggaagcagg cttcgtaaga catgccagac ctaattaaac
    16921 accccagaaa acaagttggt tagcagatgc agtaaacaag ttgttttaga gcgcatggac
    16981 gcgctctgtg tgttttagag ggatctatga cggtcgaaaa agacgaggta aaaactcgcc
    17041 tgacaccagc ggagtgggcc gaagctgaag ccaaatggac gtcaggcgaa tatacactct
    17101 caaagctgga ggaagagtac ggcattcgtc gtgaaacact ctccagacat ttcaaaaagc
    17161 gaggattaga gaaaggcgcg gactctgttg ggaagatggt tcgtgagtct cttaaatctg
    17221 acgcagagct tcgcgctaaa gcccgtgcgg aaaagataga agaacgtcgt acacgttatg
    17281 acggctgggc gtatgcgttg gggcagatgg tgatggtcga agtcactacg gccaaacgtg
    17341 agggtaagcc tttaggggcg attgaggatt ctctcaagag cttacagaga gccagtaata
    17401 cccttgcaaa atgctttgaa gtttcgtcca aagcattggg catggatcat gcggaaaatg
    17461 acgaggaaga aattccgaac ctggtatttg gtgagcttac gccttcccag gtggcgaaat
    17521 tacgtcagga agacgacgag cctgaaatca tcgatgacga attgcttgag acgttggaag
    17581 aagaagctct aagcgaattt gatgcgacag atgatggaag tgaaggggag gacgaataat
    17641 ggcaatcccg tcctcgctca gtcttgtgca actgcattct ggacagatga aagtcttcca
    17701 gtctccgcat cgatttaaag ttgtttgtgc tggtcgacgc tggggaaaat cccggttgtc
    17761 gatctccact attattcgtg cggcggcaaa ggaaaaaaag caaagggtct ggtatgtcgc
    17821 tcctacttac cagatggctc gccagatttt gtgggacgat ctacaggaag ttctgcctcg
    17881 taagtgggtt aggaaaaaga acgacaccac gatgacaatc gtgttgaaga acggttcgga
    17941 gatcgccctc aaaggtgctg ataagcctga cactctgcgc ggcgtagcgt tgcattttgt
    18001 agtgcttgat gaatttcagg atatgaaggc tgacacctgg tacaaggtgt tacgacctac
    18061 tctttcatcg acacgcggcg gtgcactgat cattggtacg ccaaaaggct tctcggaatt
    18121 tcacaaactg tggactatag gccagaacgt agagctgcaa agaaagggac agtggaagag
    18181 ctggcagttt gtaactgccg attctccgtt tgtgcctacg gcggaaattg aagctgctaa
    18241 gaacgatatg gacccgaaat cgttcgctca ggagtacctg gcgagctttg agaacatgtc
    18301 cgggcgcgtt tactacccgt tcgatcgtaa cgtgcatgta aaaccgcttc agttcaaccc
    18361 tcggttgcct atatgggtag ggcaggactt caacattgac ccgatgtctt cagtaatttt
    18421 gcaacctcag ccaaatggtg agctatgggc aattgatgaa ttggtgctct tttcctctaa
    18481 cacggcagag gtttgtgatg agcttgagag acgcttctgg cgctggaaat cacagataac
    18541 ggtatttcca gatccggcag gtgcttatcg ccaacatgct cgcggggagt ctgacgtcga
    18601 catattcaaa gagaagggat tcttacgtgt cgattattcg aaaaagcacc cgccaattgc
    18661 ggatcgtgtt aatgctgtta accgaatgct gatgaccgca tctggagata tccggctgta
    18721 tatcgatccg aagtgcaagc atttgattga ttcactggaa aaagtcatct acaagcctgg
    18781 aacacgagat atggataaga caggtggcat tgagcatagt gcagacgcat tgggctatcc
    18841 agtacatcgt aggtatccag tcaaaaaccg tgttattctt ggtggttctc ggtaggtaag
    18901 taattatcta aggttattca aatggaattg aacgacaaac aaattaagga tctggtggcg
    18961 cgacgccacc cggaatacga aaagaaaaaa gaacattggg acttcctcgc cagcacttac
    19021 gctggcgggc gtggttggtt tacagacaat atctttcgtt actttaaaga gggagatcag
    19081 gagtttaagg agcgtgttga gcgtgcctat cgcttcaacc acactcgtga ggtggtaaac
    19141 ctcatcaaca aatatctctt taaagaagac attcatagaa atatcgaaga ggcaccagag
    19201 cagatccgca atttctggaa acgtgcgact cgccagaatg cctctattga ctcatttatg
    19261 gccgctattg atttgcagtc gtctatttac ggtcgcatat gggttgttgt cgatagcacg
    19321 atgagtggtg atgttgagtc agtagctgac gagaaaaaga aagatgctcg cgcctacgcc
    19381 tactggattt cacctcagca aatgctggat gtggcatggg acgacgacgg gaatatgtta
    19441 tgggcgttaa ttgtggaagt cgctcgcgat gacgcagatc cttttacttc tacaggtcag
    19501 gaataccaac gttatcgtct gtggacacaa aacgagtggt atctgttccg tgaggaagtg
    19561 aagaaggggg ctggtggagc aggtcgtcgt caggcaaaag ttattttaga ggacagcggc
    19621 gcgcataatc tcggcgtagt tcctgtgttt cctgttgatt gtattggaga aagtgaatca
    19681 ccgtatttca gcccatcgtt gatcgatgat atcgcttatc ttgatcgtgc ggttgcaaac
    19741 tatctgtcaa accttgatgc cattattcag gatcagacat ttagccagtt ggctataccg
    19801 gtacagtcgc ttttacctgg tgatgaaaac cacactaaag tgcttgaaat gggcacaaag
    19861 cgagtcttca cctacgattc tgaaggtgga aaccagccgt tttatctgtc accagacccg
    19921 aaacaagctc agatgatcat cactacgatt aagacggtga ttaacgaaat ctaccattca
    19981 gttggtgtag ctggtgagcg aacgaagcag gacaacgcac agggaatcga taactcatct
    20041 ggtgccgcaa aaatgtatga cttccagcgt gtaaatagct tgcttgtcac aaaagcagaa
    20101 cgtctggaaa gggcagaacg tcaaatcatg caactggttg cgaaatggat gggggtagat
    20161 ctggacgaag accactcatt aattgcgtat ccagaaagtt tcgatattcg tggccttact
    20221 gatgaatttt ctgttgccga gaaactgtcg ttacttcagg caccggactc tgtacgtcgt
    20281 caccagatgg aaatgctgat tgagaagatt ttcccgaaca tcactgaggc gatgaagaag
    20341 gaatttgata aagatctctt gaattttccg ccaaaaaatg atctaaatac ccttgaaaat
    20401 aagtcagtac ttacttatga tcaaggcgca gcccaagaaa gcgggcaaga tcaaccccga
    20461 gggaatgggg actcatctac tcaagagaac gagtgataag taacaaaggg aatttttatg
    20521 caactgtggc aaatgctttt ggcccgtcgt ggtctgatgg atgttgctga agcgcatgag
    20581 cgtggaggcg ctggcggtgt agctgctgat aatgagcaga gtacacaaga tcctgacaaa
    20641 cagggtgaac aaaaagagca gccgaagggt gatgacgaat acgctggcat gactcatgaa
    20701 gagttactgg ccgaacttcg caaaaccaag aaagctggtg ctgaactgct gaaagagaac
    20761 atgaagcgca aagagaaaga gcgcacattg gccgatcagc ttgctcagta tggtgatatc
    20821 gacccggcgc gtgctcgcca gcttttagaa gctgaacagg ccgcagaaaa cgcacgtcgt
    20881 gaggcggagc aagctgaact ggagcgtcgt ggtgagttcg atgctgttaa aaaacagatg
    20941 atcgaagcac accaggcaga gctggcacag cgtgacgaac gttatgcagc actggaaagt
    21001 gaaaacgcat cactgaaatc tcaattagtc gagatgaccg tgggcgcttc cttcagtaac
    21061 tctctcttcc tacgtgacaa agttctgatg actccggcaa aagcccgcgt gatctacggt
    21121 tctcattttg aagtgggtga agacggtagc gtagtgggtt atgacaaacc ggcaggtcat
    21181 aaagaacgag ctgttctggt tgacggtgaa ggtaagccgt tgccgtttga atccgcgatt
    21241 gaacgcattg tgcgggcaga tccggaagct gacgcattga tgcgtagcga agccaagcag
    21301 ggtgtaggct cacattccaa atcgacctac acaatatccc aaccgaagaa caagtcgact
    21361 atggataagt tggcctccgg tctggggaaa attggactta agtaacatct aaatcaaagg
    21421 gaattgatag atgccattac tgcgtgaaga agctgaaaag ctgtctaaca acgaacttga
    21481 acagggcgtg attgaaacca tcatcgatcg cgatgacctg tttgccatcc tgccttttat
    21541 gaaaattaat tcaaaggcat atctgtacaa ccgtgaaaaa acgctgagtg aagctacctt
    21601 catcgacgtt aacgacacca tccctgaagg tgcagcaacc ttcgaagaat gcgttgcgaa
    21661 actgcgtatt ctggctggtg acgtagacgt tgataaattc ctggcgacca ctatggccga
    21721 caccaacaac cagttggcta ttcaggttcg tcagaaagtt aaaggtctgg ctcgtgcgtt
    21781 ccgtcgcaac ctgattttgg gtgactccag ctccaacaac aaggcgtttg acggcattcc
    21841 acgcctgatg cacgcagatc agaagatcga tatcgccggt gcatctatga ctttctctat
    21901 gttcgacgaa ctggtcgatg cggtgaaaga tcttggtgct gactgcatca tgatgcgttc
    21961 agagcacctg cgtgcttacc gtgcgctgct gcgtacagta aacgtaggcc cgtctgaaat
    22021 catgatggag aacttcggtc gtccgatgct gtgtcataac ggcgttccgt tcatcatcaa
    22081 cgacttcatc ccgaccgatt ctggcaaagc aagcatctac tgcctgcatc tgtcagaaga
    22141 gaacggcgtt actggtctgt atggcggcga caacgcaggt atcgttgttg aaaacattgg
    22201 cactgtacag aacaaagacg cagtacgtac ccgtgtgaag tggtactgct ctctggcgaa
    22261 taagcacgat aaggctatcg ctgcactgac caatgtaaaa atttaatcag tgtggtagat
    22321 aagcaattat ctatgtttaa gggtgggcta tacgcccacc ctttttgtag gagcgagaaa
    22381 tgccagaaca aaagatgaag attacggaag aggcattttc ggattttacg gggcatatgt
    22441 gccgcgccgg atttaccaat tctatctccg atgaaccttt aaccgagcgg caacaaagtc
    22501 agctatctgc ttgtttgcag gcggttcccc tctcccaatc cgtaaacata accccggctt
    22561 caccgtcagt tttggttggg aaaactgttc aacttagtgc aggtattagt atgggtaaga
    22621 gtgccagttc attcacctgg aagtcagcga atgatcaaat tgcaaccgtt aacggcactg
    22681 gattagtaac tggtgtagca ccaggcaaag taaaaattac tgcaactgat caggaaacac
    22741 agctttccgc gtcagtggaa gtcaccgtaa atccggtagc cgttcaatcc gtaacggtaa
    22801 cgccagactc aacctctgtt gagaaaggga aatcagtcag tctgaaagcg aatgttcagc
    22861 cgtcaaatgc gacgaataag gctgttacct ggtcttccaa aaatgaagat aaggcaacgg
    22921 tagaccagag cgggaacgtt actggtgtag aagttggcac tgcgacgatt gagatcgttt
    22981 cccaagatgg cagcaaaaag gcaactgcaa cggtggaagt aactgcgcct gttgtggctg
    23041 ttaccggcgt cgaaatcgac ccaaatagca caaccgttga ggcaaacaaa actgttcagc
    23101 tgaccgcaaa tgtcgagccg gcaggagcaa caaataaaac cgttacttgg gaatccaaaa
    23161 ataccgagtt tgcaacggtg gatagcgaaa ccggtgttgt aactggcgtt gcggctggta
    23221 ctgccacaat cgaggttact acccaagatg gtagccacaa agcgacagcg accgtagaag
    23281 tcactgctgc acaggaataa tcaatattgg gcggcgaaag ccgcccatgt agagagaatg
    23341 cgttatgaaa ccagcaaaaa ttgttttatt agaaccgcaa ttttccggtt attcgggaat
    23401 gttgtgcggc gttcagttcg agaacggggt gtccgtagcg gagttgcctt ttatcgatca
    23461 gcaaaggatt tgtgcctcaa tgcgagcatc aacagttgag ggcaaaaatg tttctccgtc
    23521 tgccgcatac agtgatcgtg gcgagttgac cgcagacctg attaccgagc cagcagcacc
    23581 tgacattgtg ccaatgaaac gtgggacacc agatgaaccg gccaaacaga tccagacttt
    23641 cacacgagaa gagttggagt caattgcaga caatgaaggc attgccggtc tacgtgtgat
    23701 tggtaatcag gttggtgtca aagcgaaagg aattgtcgag atgattgaag gcatcctgaa
    23761 agcacaaggc ggtgagtaat ggcgcagatc gactcgtatc gtagcggtga agctgtttcc
    23821 ctttcatttg ccttcaacgt gctggatatc gaatctgcta cctacacagt aaaggacagc
    23881 accggggcta ttctcgttga tggcgagccg ctagaaatta ccagcggtca aatgtcgatt
    23941 ccggttgtcg tgtcggctga atataaccag ctctctgaga aagaacgcga tctgcggtac
    24001 gtcattgtga aggctgttgc atcgggtctg acgcatgaag agcggcaaat gtatgttctg
    24061 ctgaatagtt ttgaactgtc gataccagaa cagtcgtttg caactgtcgc tgatgctcaa
    24121 atgcaggcga tcgatatgct gaatggggac actttgctgt cggatggtga aggcttaatg
    24181 cgcaagcgtc tcattgaggc taccagacga attaaaactt taccgttctc aatccgcaaa
    24241 attctacgta tcgactttga ccgatacgat cgccctcaaa atatgctgaa tgtgtatgac
    24301 attccgtggg gagcagatgg agcgtaccgg catgatttag tcgattggga aaagatgacg
    24361 caggagaagt ttgaagagtt ccctgactac ttcaaagaag cgttgatgct tgccgttgtc
    24421 aacgaagcgt gtgagatcgc aaatggcaat gatgtagccg cagcacgaga ggatggcatt
    24481 ttgtcagagt ccatcggtga aacaaccaac atgtaccgta ccggtaaagc tgcaaacgtg
    24541 catgtggctc gtagtacctg gcgcttgctg gtcagttaca tcaacaaccg tatgattgtt
    24601 cgccgtgcgt aacgccagtc gcattattta cttttggtct aaagggtttt tggccagtaa
    24661 tgagagcgac cgacaacaac agggagagag catgaatatt tcatggcaaa cagaaattgc
    24721 gatctaccgc tttggcgcaa agaacgttta cggtgaggcg caattacaat tcgtcaggaa
    24781 gacgaatgtc ggcgtggtta agttcgaaca gagtaatgag aaatcgtcag ttcgtgctga
    24841 tagctctggt agtcgtggta aggcgagtct ggagctgttt gatgctgtgt tagttgtccc
    24901 tttggaagcg gctgtacaac ttgatgatgt actgatcctt gaaggtcaaa aattaaaggt
    24961 gtccagtgtt catcgccgct gggggctacg aggaagacct gggcatcttg aagtaggggc
    25021 gaatatatgg gtctgaaata cgatgcgcat cagttcaaac gtgcggggaa tcgactcaac
    25081 aacagccaga aagcatttaa gcgatatctt attcgggaca tggaaaagct ggcgcgtttg
    25141 gttgagcgtc tcagccgtgc aatggccccg ctggaaaccg gatctctgga gacagccatc
    25201 tttgccaggg tgattaaaga aggttattca gggctgcgca ttgagctttc tgtgtctgga
    25261 gcaaaaccac gtcaggggca tccgggcgta gaagtaggtg actatgctaa gtacatggag
    25321 ctgggtaaat accgactcgg ttatctttct cgaatgaaga acgtgacaaa cccaccaatt
    25381 gccggtgtta aacctcgtgt tgggccgcac tttctggaaa gagcggtgga aattagcgag
    25441 aagcagttct cagaagcgat tcttgaggct gccagaaaag ccggttttac gagaggttaa
    25501 tgtgtttatc gaagcgtttg caagtttgat gcagaaggca aagattggca cggtcggggc
    25561 tgacattttc tgtcactact tgcctgccaa tgtgaaatct ggcgtcctgc ttattaaccc
    25621 aaataccggc atcagcatcg atcatgagct acagggcttt tatcacgaat cattcacaat
    25681 cattgtgcgt ggttcgtcaa ttactacgac ggttgagaaa gccaataaaa tcattgagat
    25741 gtttccagta gaggaaacgg aatctggtgg tgtgtatttt cgacttgtac gaccgatggc
    25801 gatgccaatc atttatccca aaaatgatgg ggcattaata gaagcaggca ttcctgttga
    25861 atttgctggc tatttattga attaactcaa ttggtaagta tatacttact attagcgcaa
    25921 tgaatgcgta gaattaacgg aaaaaggagt tttccatcaa tgtctaatac ccatgtaaaa
    25981 aacatcaaac ttggtgcctg taaggtgtcg tttggtggtg tggatttggg ttacaccaaa
    26041 ggtggtgttc aggttgaagt agccactgaa acgctgaaag tcaccgtcga tcagttgggg
    26101 cagaccgtaa tttccgaatt agttcagggg cgcaacatta ccatcaccgc gcctctggct
    26161 gagtctgtac tccaaaacat ggttgacctg atgcctggtt ctactctgag cgaagatgac
    26221 aactctgtca cgatcacatc tgcacagggc gttaacctga ttgatgtggc gaaggagctg
    26281 gttctgactc cgcaggacac caccgactat gtgttgacca tcccgaaagc ggcaaccgcg
    26341 ggcaacttca ctatgaccta ccagtctgat gatgttcgtg tgttctctgt tcagttcagc
    26401 gcatacccgg atgatgaagg cgttctgggt aaaatgagcg gtcctaaacc ggtaaaaact
    26461 gtgtcgatct caccggaatc tccaaccgta aaagctggag aaactgtgca actgacagcc
    26521 gaaatcactc cagctgatgc cgcagacaaa accggtgttt gggaatccga agaccaggaa
    26581 aaggccacgg tagatcaaac gggtctggta cgtggtgtgg cgcagggttc tacaaatatc
    26641 tccttcacca gcaatagcgg tggtaagaag gcaactaagt cagtaacgat taatcccgca
    26701 gattaatcag taattaagca gaggctcagg atgggcctct cttttaaaag gattttaacc
    26761 aatgaccaaa ttactcgatc tcgattccat tctgcctccg aagaaaagca tcaaatttgg
    26821 cggtcaggaa tatcccatcg ttgaaatgac ggtaggtctg ttcgtttcca tcaagcagat
    26881 ggaaggtaaa gatctcatga acatgtctcc tgttgagcaa gtgacagctt atgcagatct
    26941 ggttcgtaag gttatccctt cagtacctga cgaagttctt gaaaaactga ctgttccgca
    27001 actccagcag atcttcacct tcgctatgga agtgattgat gaagaaaacg aaaaagcggc
    27061 tggcgaaggg gcaaagtaat ttcccgcgat gaatccggga caaggaccgt ctcaatagat
    27121 ctcggattct atttcagtcg tgtagttgct cactacgccg tgtcgccatt agagctactg
    27181 aacgttcctc tcacgatgtt ctggatgctc agtcgcaata tagaccgtct gcgtgcggaa
    27241 gaggatgtcc gcaacttaca agtcgctcgt gcgtcccagg cggatggcga ggctgtgaag
    27301 gcgttcatgg agggtttgca actcaggatt ggaagaccag tcgtaacaga taaagtctac
    27361 gatccaagcc aggataaggc agaccctgac gccaaagagc aactgatgca aatatttggc
    27421 agaggatgac aagggaatgt cacaaaacgt agagtttatc ctgtcgctgg aagacaaaca
    27481 gtttacagcg tcaatcgatc gtgcggggaa actgcttact cgatttggtg agcaggtaac
    27541 aaagcctgct caaaaaatcc aaacctttga acgctctttg ggttcggtcg cccgtatcat
    27601 tggcgttctg gaaagcaagc tcgattctac ggcagataaa ctacaggatg tagctgccgg
    27661 ttttgagctt gcttcagatg ctacgcgtaa aatgcgtggc aacatcacca gcctcaattc
    27721 tggtcttaaa gccctgattg agcgcgtcga tacgacaact tcttcagtag ataaactcac
    27781 cgcgtcatta cgtaaagtgc aatcagagct aaatgatttc tctgattggg cgacctatgc
    27841 aagcaaaagc gcaagccgct ttggtacgga ggtcaaagaa gcctctgcgt ccgtgagtgg
    27901 catgaatacg cgccttaata ccacgacgaa gcgactcagt aattggggtg tcacaacgag
    27961 tcaggctgcc gagggactga aaaaggttcg tgagcagatg gacgaagtta tcggacgtca
    28021 gcaactgatt agcaagccgg tacgcgttcg tacatctgga agtggcgatg gtggtagcgg
    28081 gcagcgtagc ggcgcttctg gtcacagcgg taaaaataac gaaggaggta tgttctctgg
    28141 ccttcgtggc aacattttcc tgcttggcga gatcggagat gcagccagaa cggttactga
    28201 catcatgttt gggtggcaga agcctattgt tgaagctgcg gccgaaatgg aacgtatgcg
    28261 ggtgatgctt cgagggttga ataaggagaa gtccaaccct ggccaggctg ccgctgatga
    28321 tatgaagtac atcgtggata tggctaagag cgccccattt gcgatggaag ctctaaccga
    28381 ttcgttcgtt aaattccgct cagcaggatt agatccaaca gatggctctt taaagactct
    28441 tgtcgattcc gttgctcgtt ttggtgggga tagcgaactt ctgaagcgtg catccattgc
    28501 tattcagcag atgtctggta agggtgttgt atcgatggaa gagcttcgcc agcaattagg
    28561 tgaggcgatt ccgacagcaa tgaacgcaat ggccaaggct gctggtctga caatgggcga
    28621 gttaactaaa gctgtagcat ctggaacggt agaagccaaa aacgcactgg cacttatgtt
    28681 caaagagttg aagatgcaga acgagggagc tgcgtccgaa atgatgaaca cttttaccgg
    28741 tacgttggcg cagatgcaga cggcctttac gctatttgcg gataaagttg gccaagctgg
    28801 ttatttgtcc tctttaaaag aggggatgaa agaacttacc tctttgatga atagcacaga
    28861 tggtatgtct tttgctcact cattgggaag tggattgaca acggctgtgg acggcttgcg
    28921 tgatttggca atgtggctga ttaaaaatca agaactggtc attgccttcg gcaaggtagt
    28981 agctgctatg gtggccttta agctacttaa ggctggtatt tctggagtta taggaactgc
    29041 cagtcagatg acgtctgctt ttgcgaatat gtctaccact atacaaggga catttaatct
    29101 cgcttccaca gccgttaaac gatttaatcg ggcagcgagc atggggctat cacctatacc
    29161 atctttaatc ttcgctattc gtaatgcgtt cagtggattg gttgcgttca tggccgcaaa
    29221 tccgataggt tttgctttta ccgcagcagt aacagcagtg tcatcgctga ttttttacat
    29281 gcaggtactc agaggaaaaa ttgaggatgt cgtaaataaa gtcagagaga tacctgaagc
    29341 aatgacggcg gcagatcgcg cgatgctgga gtctgcgaag cgagagaaag atcgtcagat
    29401 tagcaagcta gagaaggagg taacaacagg taaacgatca caggtttcaa gagatgggct
    29461 ggtttatgaa acagatgttg acgttaacag taccaaacaa caacttgaaa acctaaagaa
    29521 agaacgcgat gacatagcta aaacacttgg cgaaggcatt gagaagagcg tctataaaag
    29581 cgtcaccaaa ggtttagacg atgcaattgc cgaagtaaac cagaaggcaa acgtctcttt
    29641 ggttgcaaat gctaaggagt tggagcagct taacaagaag aatgaggaga ttgccggcag
    29701 aactgaccta tctgatcacg agaagagtca attaagagcc gcgaataacg caaaaatcga
    29761 gcgtttatat tctgaggcgg atcttcaaaa actcaaaggt tatcaggata ttcagaaggg
    29821 tattcttaat gatcttgtta acttaaatga ggattttaaa aaggctacaa cggcaaataa
    29881 tactgaacag ctcaaagaaa ttcaggctcg gattgatgct cagatagcta acaagaaatc
    29941 gatagaagag cagatcgaaa atgttacgac cagccttagt gtgaaagctg gtagcggtct
    30001 taagtcaatt aatggcggta aaattggtga gttttcaagc acaccagttg atgctaattc
    30061 caaaactatg actggttttt ggaaacagca gatggactct aaaaggtatc aaagagtcaa
    30121 tctcgatggt accgcattat acgacttaca aggtcaacct gttgttggac ctcgacaact
    30181 caaggagcaa ttaagacaga agaaaattta cggtgatgtg aaattagagc agttgtcaga
    30241 aaatgaccgc atcgcagtaa ttaagactct gacaaaagct cgtgaacttg atgcagaagc
    30301 agcagataag gcagcaaacc gtaccgcaaa agcagccgag agagcagcaa agaaagaaca
    30361 ggcagcgcaa cagaaattgg cagccggata ccagaaggct ctggacaaag ccgatcagct
    30421 tatggggcaa atgggtgaaa gctctaaggc tacggtatcg tttgatcagt ctcttcgcga
    30481 tacaacgaaa tcgctgacag atttggctaa tgcggttcct aacgagttca tcactcaaga
    30541 gatgatcgac aaggcgaaaa aacgtcttga agacctcaaa aatgcgacac ctgaatatcg
    30601 cgagatgttt aatcgccgca atgttgagca gatgatctcc acttgggcac cggaatcaga
    30661 ttccattatc agtgctggct atacgccgtc tcgtgaagag aaagttgctg atttcgaaga
    30721 cacctacaac cgcaatctta aagcgttgat agaacttcgt gataaggcgt ctgatcctaa
    30781 agtcgtggcg ctttatacaa agaaaatcaa tcaacttatt gctgctggca ataccgcgct
    30841 cattaaagag acagggactg cgacgcagaa gttggcactg gaatacgaaa acctggcaga
    30901 gcagatcgaa agcacctgga ctgatttgtt tagtggctta actgatgtcc tgactgattt
    30961 cgttattaac gggaagatga gcttctccag cctgtctcag tccattttga aagatatcac
    31021 caatatggtc gtgaagtcgc agatcacgct gcctctaatg aacatgttgg ggatgggagc
    31081 caccgcagct ggtagttcac agagtggtaa tttgctcacc ggggttgctt ccgcagttgc
    31141 caatcaaggt gtgcgcataa gcaacactgt taatggcgac aagtcggtag gagaagccac
    31201 gaaggagacg tccagttcgg taactggatt ggggcaaaca acacagcaga ccaccagcgc
    31261 aattggcact gcaacaaatg cgattggtag ctgggtatca gggctatttg atagcactga
    31321 agccaaagat gccgagacaa aagccgtgaa ggactccatc ttctcgatgc agaacctcag
    31381 ttctgttacc ggcgcgctgt ctgccgcgtt tgcaatgctt ggagctaatg cttccggctc
    31441 tggtaataag tggttgaatt tcggcgcgac agttgcatct ggtttggttt cagtatgggc
    31501 tggtggtgga ttcgatggat tgatgtccgg ttcttctggc tcaacttccg ggttcaacaa
    31561 tctgaccagt tctgctgccg atggcacgaa tggtattcct gcaattccga agtttgcaaa
    31621 tggtggcata ttcggaaaag acggcgtgat cccgctccgg gcataccaga aaggcggtat
    31681 cgccaactca cctcaattgg cgttgtttgg ggaaggcgat atgaatgagg cgtatgttcc
    31741 attgcctgat ggtcgaacaa tccctgtaac gctcagtacc gatggtatga gtggaggagg
    31801 aaatgttctt tctccggtat caattgagat caacgtccat agtgacggta gcacaactga
    31861 atccggcgat acagaaagca tatggaacaa tgccgctcaa cggatgaaag caatcgcgct
    31921 tgagactatc gctcaagaga aacgccctgg cggatcactc aacccaaaca ctcaacgtaa
    31981 ctaactatcg actgccccga ccggggcagt ctcacaagga tgtgagatgg aaagacaaac
    32041 gtttaattgg tatccagatt acgaatctga aaaaagcgta aaaccgaatg taacggtact
    32101 taattttggt gatgactacg agcagcgaca ggctcaaggt cttaatcgta ttaaagaaga
    32161 atggtcgtta acctttacca gatcatacaa cgaaattaat gcaatcgatg acttcctgac
    32221 tgagcgatca ggcgttgaat cgttctattg ggttaatcca agaggcaagc agatcgtagt
    32281 tgtatgtgac agtcatacgg tcaagcgata tcaggggtac tgtgtcttaa cagctacatt
    32341 cagacaagta tttgaggctt aagtatctag ataagtaagt actaatttac tatcattgtg
    32401 gcgctgacag gatgttagcg cctacttatt tcaaggatga aacaatggga attaaagctg
    32461 atattcagag cttatctccc tctgcactca ttgagttgtt cgtactggat atgtcgaaca
    32521 caacttcagg ggggaagcta ttcttccacg ctggaacaaa cgaactgatg caaccggttg
    32581 tctggcaagg agtgacatac gagccgtggc caatcaaagc atcaggcttt gacaaaacgg
    32641 gtcagggaac gttaccacgt ccaaaaattc aggtatcgaa ctttgccgga accgtctctg
    32701 cggaagttca ggcgaacgac gatcttgttg gctgccgcat tattcgcaag atgacgctgg
    32761 ctcgcttcct cgatgccgtt aattttaaag acggcaaccc aacagcagat ccaaaccaac
    32821 atttcccgga tgaaatgtgg tttatcgaac agaaaactct cgaaactcat caggttgtcg
    32881 agtttgaatt gtctagtgtg ttcgatttga tgggggtgca actgccgtat cgtcagatca
    32941 ttaaaaacac ctgcccgtgg aaataccgag ggccagaatg cggctatacc ggtccatatt
    33001 tcgacaaaaa taaccagcag acgtctatgt ctggtgcgga ttactgcaca aaacgttatg
    33061 acgcctgtaa tgcgcgtcgg aattattttg ccgacggtgt gatccatttt ggcggattta
    33121 ttggagctac gcggtatggg taataaagca atccctgagc ttggctctga cgttatgcag
    33181 caaatctatc tctgcgccat aaatcgctac cctaatgaag cgtgtggctt tctggttaga
    33241 actaatggcg acaaatatcg ctttatggaa gcgcggaatg tttcggagaa cccgcagaac
    33301 acttttgtaa tgcacgttga cgacattatg gcggcagagg atgcgggtga tgttatcgca
    33361 atctggcatt cacatactga tgaatcagca gaagcatctg atgccgatcg tgcaggctgc
    33421 gaagcgacgg aagttccgtg gatgattctg gctattcgca agaatgttga gggagatgcc
    33481 cctttccatt ttagcgagat gaatgtgatc acacctgatg gtttcgaaat gccatacctg
    33541 ggcagaccgt atgtatttgg cgtattcgat tgctggatgt tgtgtcggga ctatttgaag
    33601 cgtgagttta acgtcgaact aaacccgaac gcacacctgc atattccatc gtggtacacc
    33661 ggcgataacg acattctcga tcagaactac cgtaacgagg gattggtgcg tcttgcgccc
    33721 ggaacagagc ctcaacgtgg ggacatcttc tttatccaat acgggaaaat gcctgaccac
    33781 tgcgcggttt atatcggcga tggaatgatt cttcatcacc agatcgaccg ccttagttgt
    33841 cgtgcttatt acggcggaat gtatcagaaa catacgacgc atcacttgcg tcacagagac
    33901 ttgctcaagg gagatgagac gtgtctgagt tagttcatgt gcagcttggc ggccctatgg
    33961 ccaaacattt tggccgccac tggcatctaa aggtgcgcaa tacaaaacag gctctggatt
    34021 taattgaggc caacaagcct gggtttaaag catggatgaa gcgcaatatc aaaacctatg
    34081 accgttacca catccagatc accaataaac agggccacaa gtggtctgtt gacgaaagtg
    34141 aatatcagat gatggggcag tctgacaaca ttgccaaaat ccgcattaca cctgttccgc
    34201 gaggaagcgg tggatctgct tttgggtggt ttcagacggt agtaggggcc gctttgttgg
    34261 ttgtatcggc ggtagtgatg cctgctttag cacctctcgg tttgtcactg atgatgggcg
    34321 gcatatcaca aatcatatcg ccgcaagcca ctaacgaaag tgtgagacag gcagataact
    34381 cgaactctta ttatttcgac ggccctcaaa atacagaaaa ccagggcaac ccagtacaac
    34441 ttatctatgg cgaggaaatt ctggttggct cacaggttgt gagttcttca atcacgattg
    34501 accagctaat gtaaacaagg atttttttga acatggaaca gttcaagaag aaaaagttac
    34561 cgctgtttat tgcaggtgcg ggtggtaaaa aaagcagcaa aagctctagc cgtacaccgg
    34621 ttgaagccga cgataccgta aattctcgtg caatggccgc tatcctcgat cttcttgggg
    34681 aaggtgtaat tggcggcttg gtaaatggcg caagatctat ttttgtcgac gatctgccga
    34741 ttgtaaatga agacggttcc tccaacttca gcggaatcac atgggatttt cgcgacggtt
    34801 cacaagacca gactccaatg tctggctttg atttcgttga aacgcctaaa tccgtcaata
    34861 tccagttaaa aaaaacacat tatgtaacgg tttcaatcga taacgatgaa gctgatcggg
    34921 ttcgtgtcat catgaagttt ccttctttgc gaagcataga taaaaaaact ggtgacacga
    34981 acggtacgac tgttgagtac aagttccaga tagcaaatgg cgactcaaca tttgtagatg
    35041 tggtcgcgga aggtgaaaaa agcgttggca ttaagctaac agcaaagaag accggcgttt
    35101 attaccgtag ctacgagctg aagctgccta agcctggacg cgcatacaag gttcgtgtag
    35161 tccgtattac cgatgataac agtagccagt atctctataa cgatacatgg gtggactcaa
    35221 taggtgagat tgtagataca ccgatgaact acccaaactc tgtgctggtg ggtctgaagg
    35281 ttaattctga gcagtttggt agcaccatgc catctcgttc gtatttggta cgtggattaa
    35341 aaattcgcgt gccgtcaaac tacaacgagg ccagcaacac ttatgatggt gtatgggatg
    35401 gcacttttaa gctgttgtct tcttcaaacc ccgcgtggat tctctttgat ttgcttacca
    35461 atgctcgata tggccttggt cagtatgtgt cggaatctat gattgacctc ggccagctat
    35521 accagatcgg tcgatattgt gacgaagagg ttgatgatgg ctttggtggt aaagagaaac
    35581 gctttgcaat caatacgcag atcaccagtc gacaggacgc ataccgatta attcaggata
    35641 ttgccggtgc attccgcggt atggtgtttt gggctggtgg catggttaac atcatgcagg
    35701 atagcccatc agatccggta atgatgttta ccaactctaa cgtcaaagat ggattgttta
    35761 cctataaagg ttctgcgcgt aaagatcgcc catccgttgc gctcgtaacc tacaacaaca
    35821 aggaggacgg ttataagcaa aacatcgagt acgttgaaga tcaggacgca atgcgccgtt
    35881 atggtgagcg taagacagaa gtcgtagcat ttggctgtac aagccgaggg caggctcacc
    35941 gagttggttt gtggctttta tataccgcca gaatggagtc ggatgtaatt acatttactg
    36001 ccggcttaga cgcgtcattt ctgatgcctg gtgaaaccgt tctgattcag aacaaatatc
    36061 gcgcaggcaa acgtaactca ggtcgaattg tggcgtttac caaaaacagc gtcacactcg
    36121 atgcgcctgt gtcgttagcc aaaggcggct gctttattcg aatactgaat caggaaggca
    36181 aaatcgttga acgcgatgtt cttgaaactg gcgaaaatat aacaaaagtt acgttttcaa
    36241 aagccctgtc ttcggctgaa acgcctgttt tgaacggcgt ctggactatt acagaaccag
    36301 acctcgaacc tatgcgcgtt cgcatcgtta acatcgcgca gggggaaacg ccgggtagct
    36361 ttgacatcac cgctgttgag aacaatccgt ctaaatatga ggcaatcgac aatggtgcaa
    36421 cgctgatccc gcagaatacg acggtattgg acccaactta ctccaagcca tctaatctgc
    36481 aaatcactga agggacttat ctctcaagcc cgggcaacct gtcagtaaaa ctgactgcaa
    36541 catgggaagg gaaatctcca gagtattgga tcagttggcg acgttctgat gaaaacaatg
    36601 tatcgaactg gcaatcggcg cgtgtaaccg aagagcaata cgaaatcgtt aatgtcgcgg
    36661 agaatggacg ctacgacttc caactgtatg cggtttcatt caacggtaaa aaaacagaaa
    36721 ttatcagtac cgtttatcag gtgctgggca caatgacacc gccggatgca ccaacgtcat
    36781 taacggccgt tggagactat cgtaatgtga tactgaattg ggttaatcca gattcggtag
    36841 accttgatca cattaatgtt tacgcatccc agaccaacaa tctggatacg gcgaaactga
    36901 tcgcagagtc tgcaagcacc acctttacac atgctggtct tggggatagt gaaacgtggt
    36961 attactgggt tcgagcatcg aacaagcgag gtatgttaag tcctccgaac tcaaatttgg
    37021 gtacagaggc gacaactcgc gatgtactgt cgttcctgac tggcaaaatt acatcttccg
    37081 agcttggtca ggctctgctt gaggacatca acagcaaagc ctctcaagaa gcagttgacg
    37141 agttaaatga gcatatcaac cagagcgtcg aatctttgga ggggtcggta aacgacgtta
    37201 aggaagatat agctgaatta gataagcagt tcagcgataa cctcgcgggt tttgaaataa
    37261 aattcaacga gcgtagcgat gctttggaga acgcacaaac cgagcttaaa ggtgaggttt
    37321 cggcaacgat cgacaaggtc aatgaagcgt ttgaaaaaat tgatgccact gatgctgcaa
    37381 ttgttgaaat cgaaaacacc gtatctgaac acgataaagc tctcgctaat acagtcgagg
    37441 caataaaggc tgcaagagat gaagcggcgg ctcttattgc taaggaaagc gaggctcgtg
    37501 ttgaaggcga tgctgcaaac gctaaacaac tggaagtgtt gcagtcaacg gtagaggaaa
    37561 gctccgctgc cgttgaagaa atgaaaaaga cggttgcaga ggtcgatcgt gcaagtgcag
    37621 aactgactac gaacattgag gcgttagcca aaacaaatat tgacctggct cttcgtcaag
    37681 atgaagacca gcacaagcag atggtcaata acgcgaagat cgcaacaaca cagaaaacct
    37741 ttgctgatga tatgtctgca atggccacga aggttgagga aattcgcgta gaaattggcg
    37801 aagacattaa ggccagtatt ctggaagagt ccaccgctcg tgccgatggc gatgaagcgt
    37861 tggcaaagcg tgtcactcag ctgcaatcta agtttgaagg agatatcagc gcggcgatta
    37921 gtacagagca ggaggctcgt acatctgccg atgaagctct gacgacacag atcactcagc
    37981 ttgagtcgaa agtaagcaac gatatcgtcg cagctattaa ggaagagcaa gaagcccgag
    38041 caacggaaga ttcggctctg gcaagccaga tcacccaact tcaggcaaag gttgatgatg
    38101 atatttctgc tgcaatacgt agtgagcagg aggcccgagc aagcggggac tctgcgttag
    38161 ccaaacaaat aaaccagctt cagtcaaagg ttgacggtga tatatccgct gccatcactc
    38221 aggagcagga ggccagagca agtgctgacc aggcgctctc acgagaaatt aacagcctgc
    38281 gagcacaaac cggaactgat attgccgcag cggttgctgt agagacaaaa gcaaggactg
    38341 atgccgatag tgcattgtct acccagatta cgtctcttac agcaaaagct aatgatctcg
    38401 aggcgtctct tgccagagaa acaacggctc gtgcagatgg tgataccgct ttaacaaaag
    38461 aggtttcaaa tttaaaagca cagacagcga aagatattag tgcggccgtt gcagttgaaa
    38521 cgcaggctcg aactgatgct gactcggcgt tgtcgtctca gatcaccaag ctgacctctc
    38581 aatacaaaga ggatattaaa gcggctgtag caaccgaaac gaaaacgcgt acagagcaag
    38641 atgctgcatt ggcgactcaa atcacaaact tagagtctca aacggcagcc aacatttccg
    38701 cggcggtaac aactgaaacg acagcgagaa cgcaggcaga taatgctctg agtgggcgaa
    38761 tcgatactct gaaagcagag gtggatggaa ataccgcgac aattcaacag caggcaacag
    38821 ctattgctga taccaacaaa aaagtatcaa ccgcgtggac gttgaaaatg gagacttcaa
    38881 cgagcggagg gcagaagtat gttgccggta ttgcgcttgg tattgacacc actgggctat
    38941 ctcagttttt ggttcaggcg gacaggtttg gtttggtcaa ctctgttaac gggaagatca
    39001 caacgccatt tgttatcgaa aacagtatcg cctatatgaa tggagcgtat atcaaggatg
    39061 gcacaatcac caatgcaaaa gtaggcgatc tgcaatctac caattttgtt agcggtagat
    39121 ctggatggcg gttcggcaaa aatggaacgc ttgagatcaa cggtaatagc ggcggcaatg
    39181 ggcgattggt tataaatggt cagcggattg acgtttatga cgataacaac gtcctacgag
    39241 taagaattgg ccgtctgtga tggtggaaaa aaaattttat atcggtaaaa ataggtaagt
    39301 atttacttaa tggcgggcaa ggatagcccg ccaaatcaag gagcatattc aacatgtggt
    39361 acagggaagg tactatcaca tttacacagg gtagcaatac tctggttggg gcagggacag
    39421 cctggaacgt aacagctaat ggtgtgttgc cggggatgat cgtcattggc cccgacaata
    39481 agctgtatga gatcaagcgc gtaataagcg atacgaatat tgttctctca gaaccttata
    39541 ccggcgaaac tcagtctgag gttccgtgcc gaatcattac gacctatgaa ggcgacttaa
    39601 cacagtttag cgcgcgcttt acagcactaa tgtcgcgtat gtcggctgat tccaagtcta
    39661 tgcgcagttg gttgactgct ctggatgagg tgacaatcga gcgtgaagac ggtacagaag
    39721 taaccgttaa gccgctaatg cagatcgtca acgagcacaa cgaaaacgtt gagtggtata
    39781 aaaataacac tgacgcgatt gatgctgctg gcgacaaagc tcgtgaggcg gcggccagtg
    39841 ctgccgcagc agcagaaagc gccaataccg ctggagaaaa agcctctcaa gcgtctcaaa
    39901 gtgcatccgc tgcggcatcc tcacaaagtg ccgcaagtgc gagtgcaact gctgcgaaaa
    39961 aatctgaaac gaatgccgca gcgtcacaac aatcagcagc cacttctgca tccaccgcga
    40021 ccacgaaagc gtcagaagct gccacctcag cccgggatgc ggcggcctca aaagaggcag
    40081 cgaaatcatc agaaacgaac gcatccttga gcgccagtag cgcagcttcc tcggcaacgg
    40141 cggcaggaaa ttccgcgaag gcggcaaaaa cgtccgagac gaacgccagg tcttctgaga
    40201 cggcagcggg acagagcgcc tcagctgcgg caggctcaaa aacagcggct gcgtcgtctg
    40261 ccagtgccgc gtcaacaagt gccgggcagg cctcagccag tgccaccgcc gccggaaaat
    40321 cggcagaaag tgccgcatcg tctgcttcaa cagccacaac gaaggctggc gaagccactg
    40381 aacaggccag cgcagcagcg aggtctgctt ccgcagcgaa gacatccgaa acgaacgcga
    40441 aagcgtcgga aaccagcgca gaatcctcaa aaacggctgc cgcatcgtcc gccagttcgg
    40501 cggcgtcatc ggcatcatct gcgtctgctt caaaagatga ggcgaccaga caggcgtcag
    40561 cagcgaaggg cagcgccacg acggcatcca cgaaagcaac agaggcggca ggcagtgcga
    40621 cggcagcatc tcagagcaaa actgctgctg aatccgcagc aacgcgcgct gagacagcgg
    40681 caaaacgggc agaggatatt gcatccgccg tggcgcttga ggatgcgagc acgacgaaaa
    40741 aggggatagt ccagctcagt agtgcaacca acagcacatc tgaaacgctg gccgcaacgc
    40801 caaaagcagt taaagcagcc aatgacaatg cgaatagccg attggcgaag aatcagaatg
    40861 gcgctgatat ccccgacaag gcccgcttcc tgagtaacat taatgctgcc agcaagactg
    40921 atatggcctc taaacgagga atgaagtatt cgacagtgaa tgcgcccgca ggagttgaag
    40981 ctggtaagtt ttaccctgtg gtcattcgtc gttcttctgg attcagcagt gaattagcat
    41041 ctagggtaac gataagtaca agttcaagaa caggaaacca cagactgaac aactgcgagt
    41101 tcaacggatt tgtcatgccc ggtggttgga ctgacagagg aaaatatgct tatggaatgt
    41161 ttcacgcata taccgcaaat gagcgtgcta ttcattctat catgatgggt agcaaagcgg
    41221 atgatctgtg ttctgtgttc tatgttgaag gtgaagcgtt tcctattgct gtatatgtag
    41281 aagaaggctt atctgttgtt gtaccatctg ccgattatgt tatcgggcaa tcaacttaca
    41341 aatggggtgc tacagaccct agaacagaat gcgtggacgc tgatactata cttgatttct
    41401 caaatggtag agggttttat agttcgcacg cattcctcac aaatgcagac attagcggta
    41461 ataaggttta cgctaatgat gaagttattg tccgtagtca gaatgcgtta aggatgattg
    41521 ctggcgatta cggtgtcata tggcgaaatg atggagcaaa tacttacttg ctcatgactg
    41581 ataaaggcga ccagtacggt ggctggaacg gtcttcgacc atttgcagta aataacgcaa
    41641 caggtgaagt gacgatcaat acaccactta attctccaaa aggggttaag gggaactctg
    41701 atacagctac gaaacttcag actgcaagaa aaatatccgg tgttccattt gatggctcta
    41761 ctgatatcac tttaaccgcc gcgcatgttg ccgctttcgc aagaagagca acagggtcat
    41821 atgctgatgc tgatggcggc gtgccgtgga atgctgaatc tggtgcttat aatgtaaata
    41881 gaacgggcga cagttatatt cttgccaact tttatactgg tgtaggtagt tgccgtacgc
    41941 ttcaaattaa ggctcactat aaaaatggtg ggttgtttta tcgttcatct cgcgatggct
    42001 atggttttga aagtggttgg gagcaggttt acacgactgg atttagacct cagccggctg
    42061 atataaacgc accaaccgct gccgacggat ggttaaatag tggtaacgga acagcattta
    42121 cgactgctca gtttataacg tggctaaata atcagggtgc attttcaaac aaatattgga
    42181 ttgcacggtg ctcatggtat tacgccaaca ataattatat agacgacact ggttgcggta
    42241 ggatcgatct atctggttcc gtgattgagg tgtttagtaa taagacaaca agcaattaca
    42301 ctattcgtgt tacaacgaca acaacatcgg gtcatggtgg cgttaataat gcggaattta
    42361 tttatgtgta caatggcagt gattactcgc caggctggcg tcgttcttat aatactagaa
    42421 acaaaccaac agcttctgat gtaggagcat tatcgctttc tggaggagcg ttaactggcg
    42481 gattaactgc tgctggcgag attatttcaa aatcggcgaa tggtctgcgc attgcctacg
    42541 gaaactacgg attctttatc cgaaacgatg gttcaagcac atacttcatg ttgaccgatt
    42601 cgggtaacag cctgggaacc tataatagtt taaggccgct tatcattaac aatgccaatg
    42661 gtgctgttac gatcggtaat ggactcaatg ttactggcgg catcaatggt agtttgatcg
    42721 gtaatgcttc aacagctacg aagttgcaaa cagcgagaaa catcaatggt gttaagtttg
    42781 atggttcagg cgatatcaat attaatacat tggtatctcg tggccgagtt acggcgttaa
    42841 gcggctctac tcaaggcact gctggtattc aaatgtatga agcatatagc aataattacc
    42901 caacatcatt cggcaatgtt ctgcatatga aaggggcgag tgctgctggt gagggcgagt
    42961 tgcttattgg ctggagcgga acggatggtg ctcatgcacc ggtttatgtt cgttcacgcc
    43021 gtgatacttc gacagcgaat tggtctggtt gggcgcaggt ctataccact gctcataaac
    43081 ccactgcggc tgatgtcggt gcgttgccga gcggtggcgg cacactttcc ggcgcactca
    43141 cattatctat ggctgcgcca agtgtccagt tacgagggca aggtacggac accagacagt
    43201 acatcatggc ttacagaacc gatggcgcta catcatggta tgtgggcaag gctaacaacg
    43261 gctccgataa tgcgatgttc tggaactata ccggctccaa cggaattgaa ttggctgccg
    43321 atggcaatgt gcgtattaat gcgaagggta aacagtttac gttcgcaaac aatgggaact
    43381 taggtcttgt tgcttcgttg gatcagagtt cagttccaca gggtacttac catcaagtag
    43441 ctcttaacac tggcactgtt ggtggtaaat cgtaccttcg aaaattccgc ggcggcaaca
    43501 cagacaccat ttggcatgag acggttcagg gcggcttact tcgttgggca accggcaatg
    43561 ctgatgagca agaagaactg tctatcagta ctggctatgg tgttcgagca agaggtgaaa
    43621 tcacttccct tagcgccaat ggtctaagag tggcttacgg gaattacggc ttctttattc
    43681 gcaatgatgg cggaacaact tacttcatgc tgactgcttc tggcgacaag ttcggaagct
    43741 ggaatgcttt gcgtccaatg tacattaaca acgcttcagg cgcggttacg atggggaacg
    43801 ggttgtcgct tgccggtggc ttaaatgtca cgagcggaaa tatccgaatc ccgacgtcga
    43861 gtacatcttg gatcgacatg agaaacaatg ccgctctgtc taacagttct gcggttgcca
    43921 cttcctctgc atcggccatt attcgtcagg agcacgccga tcgtcattat tttgttggtg
    43981 gtttaggcaa ctctcagttc gggttctaca tgattaacaa gtcacgaacc gcgaacggca
    44041 cggatgcgaa cgcatacctc cagaatgacg gtacttgggt ttgcggcggt aatggcagct
    44101 ttaatgacgt ttacatccgt tccgaccgcc gtagtaagcg caatattcgc aaaattgagc
    44161 gggcgctcga caagctggag cagatcgaag gtgttctcta cgagattcag gtctgtgacc
    44221 ggtatgagca gtctggcggc ctcatcgctc aggacgtcca gaatgttcag cctgaactgg
    44281 taacggttga ccataacgat cagtcaggag agcctcgtct gcgtcttaac tacaacggcg
    44341 ttatcggcat gttggtcgag gctgtcaaag agctgcgcga ggaagtgcgc gaactgaagg
    44401 caaaaatgta atcaaggtga atggtgaggg gccaccctca ccaattcagg atattcacat
    44461 caaaaaggag attagatgtg ccaattgtcg gtgttcccgg ttggattggg tcttcggctg
    44521 tcagtgtgac aggccagaga tggatgagtg cggctcgaac ggcggtgcaa ctgccagcgg
    44581 ctggttcaat gtctcaaatg gccgggcgat ctaaagaagt tcagtacagc atcggtgcga
    44641 atcataacta caacaaagac acactgatca actatttaaa atcccagggg gcgacgccag
    44701 tcgttgttac cattacaggg gatctggtgt cgtccagtag cggcgtgcca tgtttagatt
    44761 tcccgagttc acttaccaac tcttatatca gccttgtaat caacgcaggt gtgactgtct
    44821 atggacgcgg tggcaatggt ggtagtaacg ctgccggtgc tgctggtggt aacgcaatta
    44881 ataatggcat cggtacacgc ttgcgtatta cgaataatgg tgcaatcgct ggaggtggtg
    44941 gcggcggtgg cggcggtaac aggggtcgac tagtatttgg tggtggcggt gggtgcccat
    45001 tcggtgctgg tgggtcttcc tctcatatga gttccggtgc aactgctggt actatttcca
    45061 ctcctggtaa gggatctgtt ggtgaggggt ctcttagtgc atatacaggc ggttcgggtg
    45121 gtaatgtcgg tgctgctgga ggaagatgta atactcaagg taacggtaca gaatataacg
    45181 gcggagcagc tggtaaagct gttactggta atgctccaac ttggactaaa gttggggcaa
    45241 tttacggcgc tcatgtgtaa gtacttacct accaagtgtg ggcgttttat gatataaaac
    45301 cgccatcccg atttgacttt tcatggagga aaacatgtcg aacgagatgg cgggcgttac
    45361 aacagagcaa gttgagcgta ttgccgcgat cgttgctcgg gaggttgttg gcaaattagg
    45421 taaagagcta cgtgaagaaa ttggccagga ggtcaatgat cagctgaaaa cctactttgg
    45481 tgatatgacc ccggcgcaac atagtattca acactccaat ctggacaaac tccttaaccg
    45541 gttagattcc atctcaagtg ggttctttgg cggcattgtt tctaaaataa cgtcgttcat
    45601 tattaccgca ctgcttttgg ggttagccgc gtatggcgta aaaagtggac tgcaataaca
    45661 ggagatcaag gatgaagact ccgagaggca ttcgtaataa taaccccggt aatcttgata
    45721 aaggatcacc gtggcaaggt ctggttgcga atccagacga accgcgcttt tgcacgttta
    45781 aagaccctgt ttgggggatt cgtgcgctgg cggtgactct aattacctac cacgataaac
    45841 gtcgcgcaaa agacggcaca agtatcgata ccattcgtga agttattgaa cgctgggcac
    45901 cgccgaatga aaacaatact gacgcctaca ttaatgaggt gtctaaagcc gttggtgtaa
    45961 ccgcagacat gatcatcgat ctgcatgatt acgacatcct tcgacctttg gttgaggcaa
    46021 tcattcgcca cgagaatggc cgaggcccgc taaaaacgct gaacacctgg tatgcggcag
    46081 aagttattga ggaaggtctg cgtcgagctg gcgttgttaa gccggtgaaa accgtgaagg
    46141 ctgttcctgt aactaaagaa accgcaggcg caactgttac agcaggtatt ggtctggcgc
    46201 agctggccga tgttatgccg caggtttccg ctgctatgga taaagcacaa ggtcatatct
    46261 ctagcgggga tacagtacgc atcatcttcg gtattgccac tattgttgtg gcaggattca
    46321 ttgcctggtc gcaggtaaga aaacaccaga aagggatggt ctaatatgct aggcagcctg
    46381 atgacaaagc taaaagttgc tttgattacg ctggctgccg ttcttttcgt tcttgtcggc
    46441 gcttacacga tgggcgggaa tgcggcgcga cgagcaatgg aagagaaggc aaaacaggaa
    46501 gacagaaaac gacttcaaaa cacagtgaaa gtggtgaatg agacgagcag taaaatacgt
    46561 cagaaagatg cttctgccgt tcatcgtgag ttgtatgata agtgggtgcg tcattaaacc
    46621 acaaacagcc agcgtgttgt tctgcgatgg ggctgagcct atctatatca gcaataatga
    46681 tgtaatgact gaagaaaccg aacgccaaat cctttttcac aatacgatgg gagagagggt
    46741 ttgtggttgg tgatgtcgaa gttcccctca aatgagggga acacattatt cttcgaacaa
    46801 tttttcgata gattttgtag gatagaacaa agaacgctcg ttgttaccaa ccagctggat
    46861 aaagcctaaa cttttataaa acgccttggc tttgtcattt aacgcctcaa cgaaaagtcc
    46921 atgaatgcct acagcaagag atgcattgta cacaacgcgc attgcatgtg ttacaagcat
    46981 tgagccaaat ccttgacctt gaagggactt gtccaaagcc agcctaccca aagtaacact
    47041 tggaacattc cgataaggaa ccttcttttg tttgcttctt gaaggtaagg actccttttc
    47101 aaaacaacta cctgacaaag tgtaataccc taacactttt ggcctttctt cttgagtgca
    47161 aagcacataa gcacgaagaa tttttccctc atgctgcctt tttaagtggt tgactaaaaa
    47221 ggcgtttagt gactcttcgc cgcaatcaaa accgtttaga tcataatctt tctctccaga
    47281 gaaaatctct atcgtcgtat tgctcacgag taaactactc catgcttttc agacgattag
    47341 cagctcgttt cagcttatcg ttcggtgccg gaggattgct tatagcatcc ataaccagat
    47401 tccaggactc ttcactcaga accagtcttc gatgttgttc tataaccttt acggcacgtt
    47461 cagatgcact gctaaccata aactgggtta tgctctggtt agacattgcg gcagcttcct
    47521 cgatgatgct tttatcgtca tcggttaatc tcaaatcgat gcgctgcttt ttcagtgcgg
    47581 acatttgtac cccctgatgg cctttgatgt aggaagacca tcgctctcct ctattcaaac
    47641 tgagttttcg actcaaagat tactttaaag ggctatagcc cattatgtat gcgtcaacaa
    47701 attatgtacg gcataacacc gtacttgtaa tatagaggtc attgttactt ttttcaacag
    47761 acaaatacaa gctatgattg ccaattgtta ctcacaacct accatttaac ctttacaccg
    47821 cagccgtagg catttaggct atatcacata taagaaaaca agttgtttca gacgataatt
    47881 atatacgcaa agggaactct ccaatgacca agatctttgt ggttggcggc acaaagggcg
    47941 ggcctggcaa atccaccgtt gcccagcaaa ttgccgtttg ccttaaagtc aaaaagaaga
    48001 agaaggttta tatcactgat atagatattc agcgcacgac aacaagctgg tgtgaagacc
    48061 gtcgacagaa cgaagacctt gagctgattc cttttgcata cgttcaggat gacatcatta
    48121 agcacctaaa atcgcttcag ggtagagctg agtttgtagt ggtagatgct ggtggctttg
    48181 actccgaaat tcagcgacaa gcgatgctga tggccgacgt tatcattatc ccgcttcgtc
    48241 ctaagcgtcg tgatttgaaa tctctgcgtg atatcgatcc tattatcgac aatgttcgta
    48301 atgtaaacga taaagtgaag gtccgcgcgg tcatgaacca gtgtccggct ttgccatcac
    48361 aagtgtctcg cattctggcg gctaaagaga ttgttgagac gtttggcatc gagtctgcgc
    48421 cagtcaatct atataaccgc aacgtctatg atgatgcgga agagtctggt cgttctatct
    48481 ttgaaatgac cggtagcgag cgcgacaaaa aggcggaagc cgagtttgaa gaatttgtag
    48541 attatctgtt gagtctggag gaaaaagaat aatgtccatg aaaatgggtg acctagcaaa
    48601 gcgcaaagag cctgatgcac cggctaagaa cacaactcct ttgcgccaac cagtcagacc
    48661 acagggacgc ccgactcgtg gcaaagagaa aattaaaagc cgcacaatgt cactggagga
    48721 cgaatacttc gaactgctgg agatgatgaa gttcatccct cgcttcgaga agttcactcg
    48781 ttctgacgtg attcgagcag ccattttcca tctggcagag aagtcaccgc aggaaatcga
    48841 ggacatcgtg aaattgaatg aggcgatcac cgctgccgat gtcacgatgc gtaccgatga
    48901 aatcaaacga gagttgatga agaaaggtta aaaatcatgc attggcgcgt acattgcgcc
    48961 aatgcataac tacagtgtca gctttatgcc gcgctaacac tatgttcagt cctaaccacc
    49021 ccaaccttca caaaagggct accgtagctt ggtggcttct tgattaattt gcctacatac
    49081 agttttctaa tatgctcatc ggctattctg ccaacaaatt cataacgttt tgtgtcgggg
    49141 ccaagagcta tatcccttgt aaagtactgc tgagaaccgg ctttgaccca gcattcaatc
    49201 tgataaactt ccattatcag cccaccatat gtagcgtagg catatttgag attctcgtct
    49261 cttggaacct ttgcccatac gccacgcgta gcttcatata atgccagagc ggacattcct
    49321 gacttgtagg tgctgtttag caggaaggca agaccggcgt gctcaggagc aatttcagtt
    49381 tcctcttgca gcactaagtg atggtaggcg tccagtgata ttctgcccat catggaacca
    49441 cttcctcgta ccttattcgt aagctctccg acccccataa ggtcgatgca tgtcgcctca
    49501 acaagtttgg ctgtggtttc atccatacca tgacgaagta tatctatgcc taatttttta
    49561 ttagccaaaa gttctttgat ccgcatggat ttgggagagt catcgggata cttgatgtga
    49621 tcgagacaac gagtcgattt gccttttcct atgtaaaacg gtcttttcat cttgtcttct
    49681 gtatcataga ggcagtaaac atagtattta gccttatcca atgatctcgc gtataccgat
    49741 aaatcgtcca ttattgcatc tctttgctac tgactaatgc gttctatttt acacataagt
    49801 ttttaatcaa tttaacaggc tcaaagtgta gagcttctgg gcgtctaaga cgacaattta
    49861 tgcctgcttc tgtatatata aataataagt aacttattaa atatatacgg aagcaggtct
    49921 tttaaaagac accaccagaa caactccctt ccgtttccac ttccaaaaac tgccaccagt
    49981 cgctatcatc cgctcattgt gataagtaag taactaccta ccaggtgagc cacatgagcc
    50041 aaatcttttt cgataccatc gacaacgacc agtacgactt catgacagag tggaataccg
    50101 ctgttatgga caagtgggtc gctgaaaaca ttggtttgtc gcgctgtaaa gacgaggctg
    50161 aactcttcga gacgaagtgg tttgattacc gcgacatgca tcctctcatg gccacctgtc
    50221 tttttacgga ggcatacaaa cgtcagtact caaatatcat gctgacgcac ggtcgcgaac
    50281 actttgaaac agctccgttc accaccgggt taaaacgtct gccttatcag gagttgtcga
    50341 ctgccaataa aacgtcgcta tggaaagcac gccagtttgc ggatcgctat tgctgctcat
    50401 acgactactt tatctccacc gttctttccg cagctgcacg acggctgtgg gacaagctgc
    50461 cgcgcccaca gcatttgtgg cagccagaac tgattgagat atttgaagag aaattagcca
    50521 gacgcgcaac aacccgtctg gatgactctc ttgttagctt taagcatatg ggagacatgc
    50581 agttcaaccc gattcaggaa agctattttg agtggattct ggagcgtttg cgcaccatcc
    50641 ctcgcagcaa gcgcatacgc gcaattttct ccgctatctg gctaatggaa atcgttccag
    50701 agcgccttat ttccgcccac tttccagaag aactggaaga agcacggcgg tttattgatc
    50761 ccctatctaa ttaactaata ctagaaaaca atttgtttaa aaaacaaagg aaagcacatg
    50821 accgaacttt gccatacagg acgcgggctg tccgaagagt ttgatgaaga ttttcagaac
    50881 agattgacgg cctatttttg tcgtgatcac gagtttctta ctcgtgctgg agatctggtt
    50941 gtacctagcc aatttgccaa tgcggccaat gccatattgg ttaatatggt ttcgggctat
    51001 taccgtatgt acaagagcgc gccctcttca tctgcgattc tggatatgct taagcgtgcg
    51061 aaacgcgata agactatccg tgaggaacta ttcgccgatg ttgttgctgc gtttaagcgc
    51121 atccttgcag aaaaattgtc tgatacctcg tacatggttg accaggtatc aaccttcgca
    51181 aaaagcgtag cgtttgatga tgctctgatt aaggctgctg aactgaaaga gaagggcgac
    51241 tttcaggggg cgatggcaat catggccaag gttcagcaga ttggatcgaa cgaagcgacc
    51301 ggaatctatg actactacac ctccgcaagt gagcgattga aagcgcgtga atatgaggct
    51361 tcagaggagt atgtgccaaa cagcattaca actggactcc ctctgctcga taggttgctg
    51421 taccaaaaag gctgggcgaa gcgtgaaatg gtgctcttca tggggttcgc taaatccggt
    51481 aaatcgaccg caatgggtga gttttccata aacgcaacgc ttgctggcta caatgttctg
    51541 tatctctcgc tggaggttca caccaccatt ttatccgacc gttttgatgc aagattgtcg
    51601 gagacagaaa tgtccaagct ggtggaacgg cgcgatgagg ttcatcgtaa gttggcagag
    51661 ttgggagcca cgaaggggat tggtagtttg tgggttgttg agcgtccgtc aggaagtatg
    51721 tcaccggcag atctggatcg tatgcttaac agcatgaaag ccaatggcat ggtgcctgac
    51781 atggttgttg tcgactatgc agatttgatg cgtgccagtt atgaccttcg tgatgatcgc
    51841 gccaacatac gtagtatcta taccgactta cgtgctcttt atgacaaaca caacgttgct
    51901 ggtatcacag catcgcaaac aaaccgtgaa ggtggcgcgt cagaagttgc cacaatgatg
    51961 cacgctgccg acaacatcga aaaagtacgt attgctgacc tggtaataac gatcaacaaa
    52021 acagaagaag aagaagcgaa aggagaggct cgtctctact ttgctggttc acgtaaccag
    52081 cagggaggga tcagcattcg cgttaaacaa aacctcgaac aaatgcgctt cattgagcga
    52141 atcttagacg ttacctaaaa aataagcgtg gagaacgcct ccacgcttga ttcattggtg
    52201 aaacaacttt tcttttgcca aaccacaaaa gaaaaacaca tgagccttta tgttatatca
    52261 acatttaggt tggtcacaat attgcctatt aaaagtggaa ttatcgtgag cgagctgaaa
    52321 gagctaatta ccgaattaga ttttgaacaa tggttggaca ctgaaggtat cgtttatcga
    52381 cgtggaggcg tgagtgctcg cggtcgtgaa gtgaatatca aggagtgtcc ggtatgcggc
    52441 agctccaact ggaaggtata tttcaatctg accagtggcg tcggtaaatg cttcgctggt
    52501 gatcatcccg aagagattca gttcaataag ctggtcttcc tcaagcacta cagcggcaaa
    52561 tcacgacgac agttcgagga atatgtgcag aacgcccttc tttcccaggg gtgggcacca
    52621 aagaaagaag agatagtgct tgcaagcaca gtcgagttag agggaccact tgcactccct
    52681 cgtcattacg aacttcctat agatggccgt cttccagact atctggttga acgaaacata
    52741 tcgcctgaaa tggcaaagta ttttgaccta cgatactgcg tcgaaggtaa gcacgcctat
    52801 gtagatccgt atacagatca ggttaaagga cagatattcg atatgcgaat actgataccg
    52861 gtttacgatc tggatggggt aatgaagaca tttcagggac gagacattac cggtacagca
    52921 gaacgccgct acctctttcc tatgcagctt ccagcttcag gtaagtttct ctacaacggc
    52981 cataatgcag tcggcaaaca gactgtagtt gtctgtgagg gggcgttcga tgttatgggg
    53041 gttaaacgag ctatttttga cgaagaaaca ttacgtgatt acgtggaacc aataggaacg
    53101 ttcgggatgc atctatctgg taacaccact caggatgcag aagatcagtt aggcgcgttc
    53161 ctgacgctca aggcgcgtgg gttacgtaat gtgatcatga tgtgggatag tgaaaagcaa
    53221 gctatacgta atacgatggc cgcagccagg cgactgacca gtattggtct taatgtcaaa
    53281 gtggcgtgtt tgggcgagga aggactcgac ccgggcgatg caacgccaga gcaaatcatc
    53341 aaagcctatt atcgggcaaa accgtatacc aagcagttgg agttacaaag caaggttttg
    53401 ggcattaagg ctctatgata acaatcacct ttaaaataag tagatgatta cttatctttc
    53461 tgtgagaata ctttcatctg ttagctagga gttggtatga aagacgaaat tcagaaatta
    53521 gcctgcgaca tcattgataa aactggttta gaaatcagcg agagcaatcg gctagacatc
    53581 attgaaaaag ccgtaaaaac agcaatggat catatcgcca ctcgtttggt cgagatcccg
    53641 ctaccagggc taccttatct gaaagttgag ttacacgtat ggggtgaatc ttcttgttca
    53701 cggcgttctg cattagttgt ttttattagc aaagaaaacc cgctcagtct taaagtgcag
    53761 gttggtgcat ggatggatgg aaaagtgatc tacacaaata ccgttttttg tctttcaaac
    53821 gacgaaacta ttgaagcggc cattcaagaa gcagttctag caatgcgcag cagcggtttg
    53881 atgaagaata actacgaaga gtacttgcgt tcgataagtg gtgaaaagac attatctctg
    53941 aaagcagatt tcgttacccc gaaaaacctg ttggaagtct tgcttaataa aggggctaat
    54001 gatgccgtaa atgtaatcag agagagtgag tatgcgtctc tttgcgacat gtgcaaaagc
    54061 cagttggatc tggtgcatat cgttattgat gctgggaagg catgtgatgg cgtaatggcg
    54121 gaatttgctt ggaaggtggt caggattgct aacgaattac cgatgataga gcaagaggcc
    54181 aaatcatacg ccaccaatca tgtcacagag cttcttgccc cctatcgctt agaaagcaat
    54241 cagcgcaaga tggttagctg gggaagttgg taatctctcc gcgcgtcgtt ttttacgcaa
    54301 ataacgatag gtaagtacaa gattatttat ggcggtagtt gtgaaagctg atttgtcaaa
    54361 aatcccttct atttcaggaa ataacggtta ttcacttcgt tgtgaggaag taaagataaa
    54421 cggtgagtcg gcatattgca gctattccgt ttgccagcac accattcttg ccttcaaaga
    54481 aaaccgtctt cctcgaactt ctttccagtc gtgcgcaacc gctattaaag caggcaaatg
    54541 caaggcgtta aaaatgatgg ttgaagagat tcgtaaagga gaatctctgt atttcgaaga
    54601 tatgaccgcg ctcattaagg aggttgaaga acggaataaa caagccagaa ctataaaacg
    54661 aaaacgtgac agtgtaacga ttaatagcat ggttaagaag agcaccacat cacaaacagc
    54721 gatcactgac gtgtatgcgg cgttgcttga agaaacaaca aaagaaacac atgagcaaat
    54781 cgatcaacat atggaggcaa aacaacaatg aaaaagttga tcgcacttaa gcataagctg
    54841 gacgaaatga aagctatggg aaccaatgca aaaaaagagg cattgaccaa catggatgac
    54901 ttcgagcaaa gcatggtttc attgatgctc aaccctttca tccgttttgg ggtaaagaaa
    54961 tacaaagtgg cagagccgct tagtgagtcc gtcccaagtg acgaaaaagc cattgatgta
    55021 ctgaataagc tggcctctcg cgagctgacg gggaacgcag caatagctgc tgttgagtct
    55081 atcgtggcgt caatgtgcgc cgatgggcag gacgtgttcc gtcgtttcct cttaaaagat
    55141 ccgaaagctg gcgttgggat tagcctatgc aacaaggttt ttgaaaatcc cattccgaaa
    55201 tttgaggtgc agctggcgtc accgtataaa gaaaaaggcg acaaataccc cttcaagcca
    55261 aatcctaaag ccaaatggcc gatgattggc agccttaagc tcgatggttt gcgagtaatt
    55321 tgtgaggtta ttgttgacga ggaagaggtg aacttccttt ctcgtactgg taatccaatc
    55381 acgtctctcg atcacctaaa gccagccatg ctcgaattag gcaaactttc aggccacaaa
    55441 cacatcttct tcgatggtga aggaacagcc ggttcattta accagtccgt atctgcattg
    55501 cgcaaaaaga acgtgcaggc aattggcgct atttatcatg ttttcgactt cttcctaccg
    55561 gaatggcggg cacaggctaa atccaaagag tatgcaaaga caggtatgaa gctgaaagag
    55621 cgcctggcta ttctcgtggc gttgttcaaa aacgatcgca gtgaagacta cgcgcaagac
    55681 attcacctac atccgttcta catcatccat agccacgaag acttcatcga acgcttcatg
    55741 aaacgcctgg acgataacga agaaggggag atgggcaaag atccgaactc tgtttacgag
    55801 tttaaacgta cccgcagctg gtggaagtta aaagacgaag attcagaaga tggtgaaatt
    55861 atcgactttg agccgggcga cccggactct ggttttgcca acacacttgg aaaaattgtt
    55921 attcgtcttg aaaacggcgt catcgttcgt gctagcggca ttaagcataa atatctggat
    55981 gagatctgga acaacaaaga gaagtaccgt ggtcgtattg tcgaggttca ttgtcacgag
    56041 aaaacgcctg atggcagctt acgccaccca cgactgaaat ggccgcgttg cttacgcgat
    56101 accgaagatc gaatcggaga taaagaatga tcgtattaag taaaagggag aaagaaacgc
    56161 ttcatgaaat cagtaagtgg ccggagttcc ctgagtactg gaagcctaaa acgcgagcta
    56221 agttagagcg tttagggttg gttgcaaacg tttctgaaac gtggtgctcg gccaactacc
    56281 agttaactga taaagggaaa gtattgctac agcaattagt agaatcagga gtgttaaaat
    56341 gattccatac atctcattag cttttatggg tggcttcctt atcggcttcg gcatctgtcg
    56401 tgatttaatt aagcaggaac ttaaaaccaa aacactgtgc atcggaaagc gtgtgtatcg
    56461 ggtagttcat gaaacaaagg tgaaaaaatg agcaatttaa cttcttggga atggtggttg
    56521 gccacctatt tcttagcggc cggagtcgca ttcgcctttt acgtaggtca gttagtcgta
    56581 aaactgttgc tgattaaatt tgctagtcat aaacgtatcg atgatggtct gtggcgtctt
    56641 ggcaccctgg tggaaactcg ctacgggcaa cttaaggaga acgaaaccat tactatccaa
    56701 gcgaaacgat tcactgccac catcacaaga acacctagtc gtagagtggc cttgatcaaa
    56761 aaagtcacaa ccgaataaaa acatggtgat aagtatttac ttacttatct tttatgtata
    56821 agatgacttt gttttcgttg agacgcgact gtttgaactt aaatacaagt gcaaacgaag
    56881 aagtctatct ggcagtagcc taataagcca aacaccagcg aggtcagttt ccagcctcgt
    56941 taccgaaatg ggacacactg agcgagtgtg attgcagaac gcaggaggga acatgaatgt
    57001 tccctccgat gaagtaacag aatgggcggt tggtatattt tcaactccat atgactcccg
    57061 gattcttagc cactgaccgc ccatcctgtt acgtcatttt gttcaattat gtcgtttata
    57121 ctgggttaaa aagcggcgac gtagcccggc tggtatggtt agccagcaca caacgttgag
    57181 gccattacat ttttatcaat tctaaggttc tattcacaga gataccggcg agcgttgata
    57241 tgtaacatgt tgggcaaaca ttcaatcgga gtagtggcct caacgttgtg aagacaggat
    57301 tgttgtgtag gtttaaccac tgttgccatt ggtgcctgtt ttcacaacaa atgattccat
    57361 acatcacatt gtataaatta caaagtaggt gctgtcctca gaaacatcat ctacttaaag
    57421 attttgcctt ctactattga gcgaagtcga aagcgtctgg cactaacgaa aagtgcaagt
    57481 agcggtgcgt ttcctggcag aaactaaacc gtcgcgattg gcactgttga gtaataaata
    57541 ctggcagtgc tgaattgatg gtgtagctca gcggtagagc agttggctgt taaccaactg
    57601 gtcggtggtt cgaatccacc caccatcgcc aatgccggtt tagctcagtt ggtagagcgc
    57661 ctgccttgta agcaggatgt cagcggttcg atcccgttaa ccggcaccaa cacaacaggt
    57721 aagagcattg ggcgaatcgg cgatactgac ccacaagccc gtaaatcgat agagtcagac
    57781 cagtgctctt accgttgtga ggaagtgcag ctctttgaag caaccagaag ataagcatct
    57841 ggcttcacaa cacaacgata agatcattac ggttaatcgt cgttcatgtg cacaatgact
    57901 ggtcgaaagg tagtgatctt accgttgtga tgaatgcaca ggctgatgtg ccgcaactac
    57961 agtagtgcgc gctttgcggg gcttgctaca accctgtgtc ggagttcagc accgaccatc
    58021 acaatagctg gagagtaggg agcatggtgc tcaagcggtc ttgaaaaccg tcccattgcg
    58081 caagcgatga tggttcgatt ccattactct ccgccagaca cagcgttgag cggtttggcc
    58141 ttttaatcac ccagattaag actccgctaa cataaaccag accgctcaac gctgtgatag
    58201 acaattacgg cagacgttct taaccatagc ttgctaacat cctagcaaca ctttttttca
    58261 gcgcaaaatt caaaggggct tcggcccctt ttttgcaact gaaagagtca agtctacgaa
    58321 ctttgtagga agaaataatg accagcaaag ataacacacc actaaaagtc acccctgaac
    58381 acattgaaca actcattcaa agtgaacatt atttcactgc atatgatgcc agccacgggg
    58441 ataacttcat ctctgtttac aattcaaaaa cagatatcga caaaggtcat gagtcgttaa
    58501 aactcttaac gttctgcgtt atggttctca aaaatggata taccgtaacg ggaaaatctg
    58561 cgtgcgtaag accagaaatc ttcaactttg atgttggtcg cgagtgcgct cgaaaagatg
    58621 ctatcgatca aatctggccc ctggaaggct atctcctgaa gcaaaagtgg catgaggcaa
    58681 agcaatgacg atcactatct acggacgaga taactgctca tactgcaaac gtgcggtcga
    58741 gctggcgaag caactaaagg gacatggctg cggtgattat gagtacatcg acatcaccac
    58801 tgccggtatc gacaaggaaa aactaagtga aattgttggt aaaccggtag agaccatccc
    58861 ccaggtgctg atcgatggcc agccgattgg cggatacaca gaactggctg catacgtcag
    58921 caccctctga ttttaacggc tcacaggagc cgtttttatt cccaccaaac tcactcccat
    58981 ttcccttaaa attcaaaaaa caacgtcaaa atgattccat acctactatg tatggaatca
    59041 ttagtaaaaa tgagttactt ttactcttga tcctataaga atctatgcct aatatactgt
    59101 ttacttatac agtacatcgg cgtaactcgg tgattgtcat atgaaaaata gctttgacag
    59161 agcacgcgct gcggagaaca cctcaaaaga ggcgattgag tatctcgaaa gagcatctca
    59221 aatgcaggcc gttatgatct cccaggtcag caatgacatg agattctcgg acgcattcat
    59281 gttattcact cgcttatctc tgctgataac cagacgtcgg ccagagatcg ctgttcattg
    59341 tattttgata catgtattgc cgcacattgc cgatgtaaaa gtaagtgaca ttaataggtt
    59401 catggtgaac caactggtca acccactaat actggatggc aaaattgtta tgggccgccg
    59461 cgttttctct ctgatgaagc agttccttag ctggtgcgcc ttccagggga tgatagacgt
    59521 gtcaccgtta aacgatatgt cacttaacaa agttgccggt ggcgcaaagc ccacacctcg
    59581 cgagcggaag ctgaccgacg cagaggtatg ggtgttctgg aatatatggg actacttcaa
    59641 tgtgtgcgct ggtacaaaat gggcggccag actgtgtctt gtatccgcaa gacgacctga
    59701 cgaagtactg cgggctaaaa aaagtgagtt caatcttaag cgtggggttt ggaatcaagg
    59761 caagaggaac aaatctgccc gtgagcattc tctgccttta agctcattaa tgcgcacttg
    59821 tattgaagag ttgttcgaat atggtaaaga cagccagtgg ctcgtgcctt cgaataaaaa
    59881 aatcgggaaa gaccttccta tgtctaaagt ggcaatagcc caggcattac gtcgtattct
    59941 ggaacgacca gaactgatgg agcttgagcc atttacaccc cgagatttgc gccgtactgc
    60001 gcgtagttac ttcccagcat taggcataag ccaggaggta tcacgtaaaa tcatgaacca
    60061 cagtcttgag gggatagatc gggtctacga ccgtcacgat tatatggacg agatgcgaga
    60121 cgccttggaa agtttctcga cgtacatcgc atcaatcgta gagcaaccgg atttagacga
    60181 aattgaccac aaatttaagg gagatcgtct atcaacagag cttattcgtg taaatttttc
    60241 atagagactt tatggcctca acaacctttt gtgatgcgcc tttctcttta ccgaatcgct
    60301 cgttatatgc agcaagaacc tgtttttcgt cctcgttaag aggagcagtg ccttctttgt
    60361 ataaaaatgc tgcgagttcg ggttggcgtt cttccagcac catcatcata agacgatttg
    60421 gctcaatacc cagtgccagc gccagcggac gaaccttatc gataggcaaa ggaattttgc
    60481 cgcttttaat taaagaaagg ttgttggcgt ttttataccc aattgttttg gctatctggg
    60541 cctggctcat aggtgaggat tcaatcaacc ctgcgataaa agcagcgtag cgactttcta
    60601 taaattcaat cttgttatca gacatggtta caacctttgc gcgttcagtt ctctctggta
    60661 agtgcttacc gatattacat caaaggttag ggttgtaaag ctattatcat ttttttcgat
    60721 aggcacttaa aagaccggtt aaaggccatt gcacggagaa aaattagccc aaaataggta
    60781 agaaaatcaa cttgcatatg atatgaatgt attcagtatt gatacaaatt ttagtagtat
    60841 tccttaccat agtataagtt agaatggatt gattgaatga aaaccactat ttccagccta
    60901 atcgctcttg agatcggaca cgtacagaaa ttagctgatg agtgtgtagc tgacatcctc
    60961 accgatctac cgaatgagca gattcaggtt ggtgtgaatg acacaactgg atttatattc
    61021 gaacttaaca acaaacgctt cacgcttctc aataccggct ccgggtcttt agccgtcaga
    61081 atctgttaac ccctcttctc cctgcgcgaa tggcttagtt ccctgttcgc gtagtgctac
    61141 attaaacaca ctagtaaata atttgttttc ataacaaagg attagccatg tctaaaaaac
    61201 gttccatcaa agaggttcag gacttccgtg acagtgtaaa acgagtagtc gctctccttt
    61261 caggtaaaaa catccctgtt gcagaacgag gggacgacgc ttatgtacgc tataacgatg
    61321 atggagagcc aattctcgta aacatcccat caatcccgga taacgcaaca ccggcattga
    61381 tgaatgctgt gcgcggattt ctcgatcatg aggttgctca cattttgttt accgatattc
    61441 gtgtgtccaa caaaatgaga gaaaaaggac gcgttccttc ctggtcgcta tggaatgcct
    61501 tagaagacgt gttcatcgag cgaaaaatgg gtcaggtctt taacggaaca agacgtaatc
    61561 tgatggcaac tcagcgcctt ataatcgaaa aagtctttaa accaaaggct tcagaggcta
    61621 ttgcttattg tggcaaagat cagcgcgcgc tttttctaaa cttctttctc tgtccggttg
    61681 taagagcctg ggatggccaa gcaccgttcg tagatttcat ggatgaatat tggcctgtca
    61741 ttgagaaacc aatttcatta ttaaaagaac atggtatcga tgtggccgtg cgtaacatgt
    61801 cttgcaccga ggattgtgta aaggtggctg cgaccatagc taagatcctc aaagacactg
    61861 aaagtgaaag caaaggtaag gagtcagctc cgggaaaaac ttccgatcct tcagacgctg
    61921 accagacgga tgcctctgga gaaaacaatg aagacaacga agatcatgag acaccctcat
    61981 cgttagataa tcacaaatct attaaatcag agtcacatag taagcacaaa catgataata
    62041 acgacagtga tgattcagat aatcctgaat catcagaaac aatattcgat gatacagaaa
    62101 atgataaagc ggtatcagat tctgatgctt ctgataacgc ggcgtcagaa tcattaaccg
    62161 ctgaccacga aaaaagaaaa acgacagaag acggctcttc agacattcca actccgtcaa
    62221 aaatgagtct ggaagaggct ttagaggagt tggatagcat ggaagatgaa gtcggaggca
    62281 tgacagaaga tgcgctatcc gaaacgatta aaagcgagtt aacagaaagc tcgaaaagcg
    62341 aatacaggcc atacaatcgc tcatacgact tcatcggctc gattgatcag gcagaagccc
    62401 atatcaaacg gcttattaaa acattctccg atattgattt aggtggatat ccaatcagcc
    62461 gttatcgcat cgttcctgaa ggcaaccaac tcttcgacaa atatattgaa aagcaccttt
    62521 cgtcaggtgt ttcgtcgacg ctggcaaaag acctggaacg tgcaatagca agcagaaaca
    62581 gagttcagtt tatacctggc cagcgtcggg ggcgcattca tggttcaagt atctacagat
    62641 taacaatgaa tgatgatcgc gtgtttcgta aaaaagaaga atctaaagcc gttaacgcct
    62701 gtgttcaaca agtgattgat ttatcgggtt caatgagtgg cgaaacgata aaactagctc
    62761 ttgcaagtgc atataccatc gccgatgcac ttgatcgaat aaatgttccc aacattatca
    62821 ccggcttcac tacatttggc agtcatatgg cagcaggaga acttaaggct atcaaatatg
    62881 agttctctcg ctttgaatct ttaatgctac ctatcatcaa aaattggaat gaaaaagcaa
    62941 attctcgcga agttcgctca cgtatggggt gcgtaggcta cacattccca cttcttaata
    63001 acgtggatgg tgaaagcata gccagccttg catcgttatt ttccggtcgc ttggaggaca
    63061 ggaagatcat gcttgttctg agcgatggcg cgccgtgggc tgttgggaga ggttttgacg
    63121 cccatttgcg ttcggttacg aagcaaattg aaacgcagac tgacattgat ttgatggcaa
    63181 ttggcatcat gactgatgca ccggagagat tttacgcaaa tcatgccctg gtaactagtg
    63241 ttgatagtct tggttcatct gtagttactg aactatctcg tatcattttg aagtaattaa
    63301 aacagcctta atgataagta actacttacc atagttaatg gtatatttat ataagaactt
    63361 gaacgctcat tagagaacaa aggaaaagcg catgactact actgcactgc aaaatgataa
    63421 aaatccttct gattaccttg tttgcaagtg gtgcggcaaa tcatttcact attttaagtc
    63481 ccatgtagcc aatggtaatt gcgagggcat tcctgagtca gtaaaagatg ccgatcctga
    63541 caccgtactg aaaatgtaca caacgcagtt tccagatgaa ccaacgctat cgaaaaaggc
    63601 acttgatgca attcaagcta aacgtgccga gcaaaaaagc gaaatggcga aatcttctgg
    63661 cttgaccagt agcccaggct acacaggcac agttgagtac aagacagatc tggtcgcagc
    63721 tcacgaactg ctaaacgtaa cggtggaaga actcggaaca aaacgtggga cgccgctcat
    63781 ggttagcgtc aacgtcaata cgccgtatcc agagttcgtt ccagaagtga agaagggcta
    63841 cgtatatggc gacttcgaac tgatcaaaga tattttcatg atgcttgaac ttggcatacc
    63901 tggctatttg tggggtcatg caggaacagg caaatcgtca ttgcctacac agctatgtgc
    63961 tttgctcaat cgtccgttga tccgtgccca acatacagca tcaatggaag aggcacatgt
    64021 tacggggcaa attctggcgc gtgatggctc tacgtatttc gagcctggct tgcttgcgct
    64081 cgcaatgaag catggctggg tttacctcgc ggatgaatac gactttgcgt ttccacaaat
    64141 tcttggcgtg tatcagccag tgctggaagg tgaggcgttg gtcatcaaag aggcaactcc
    64201 agaatggcgt cgcattactc cgcatgaacg gtttgctttc attggcactg gcaacacaaa
    64261 cggttctggt gatgaaaccg gcttgtacca gggtacaaac atccagaacg ccgcgaactt
    64321 ttcacgtttt ggcatcgttt cgaatgtgaa atacatgagc aaagaggcag agatcaacat
    64381 gttgataaat gccggcatcg tggatgaata cgctgaaaag atggttaagt ttgccggtat
    64441 cgttcgcgat ggatatgaag aacaccttat cagccagcca attgggcctc gtgagctttt
    64501 gttgtcggcc aagattggaa tgatgcgagg cgactttgtg acaggtattg agcgttcttt
    64561 cattaacaaa ctcccttcag cttctgcgca agcggctcgt gaagttgttc aaaaaatatt
    64621 tggttaatcg tgcgtaaagg atgtttcggc tctcttatcg ctgcttctga aactggcaag
    64681 gcttgtctgg ggtgtccaga caagcccgat tgtcaccaat cagcaaaaga agttgcgatt
    64741 tcgatgtatg ggaagttcgt aggcttcccc aatgacaaaa tcaaaaaaac cagaaaggta
    64801 aaaacacatg aaggcactga tggttcgaac tgacttctca cttggggagt cggctctaaa
    64861 agcagaaaac gcggtgaaga ttgccagaga agctggctac accgctgtaa tttcagcaga
    64921 tagcatgaat attgcaagtg ttattccact acaacgtgcc gctggtgacg acatggcggt
    64981 tatttgtggt gtgaaactaa acattgttga tgatcccaca tacgagcacc gggctaaact
    65041 tgctaaagaa tctatgagat gtatggaatc attagagcgg ggacgtaact actcgtttac
    65101 cgctctaatt aaaaatgagc aaggatatcg cgacatctgc gaactaatga cggcggccaa
    65161 cacacgagaa cagttctact ttgtaccgcg tctctcgctc gaacagttgg tttctacata
    65221 tgccaaaggc aacatcatcc tgcttacttc cgacatcggt agcgtgttcc aacgcaacga
    65281 ttttgcaaaa atcataagca cactgattac agcgggtgga aaagacaact tctatagtgt
    65341 ggtttatccg caccctaccc cattctacga ccagattaac gtccgggcga tgaaagtcgc
    65401 cagcgcattg aaaatagagc cagtggcgtt ctatcccgct tattacgaat cgatcgacga
    65461 tgcagacatt aaagacattg cgcacatggt tacgaacaac atcaaaatcg accagccgca
    65521 tcgtctgcgt atcccccacc agcgagataa cgccgtcaat ggtcgccgcc atctccttga
    65581 ggcgcttaaa gccttctccg ttcgcatgga tgtgccggta acagctgcaa tggcctcaac
    65641 aacgcaggat accattatcg atgcctgcac atggcgctgg catgaattgc cgccagcact
    65701 gccaaagatg gcagacgacg agcctgcaac gcttatgaaa ctggctgttg cagggctgcg
    65761 taaacgtctt accacaaaag agtttggcta cacaccacct gcttctgaga acagggttta
    65821 tgttgagcgg ctaaagtacg aaatggacac gctgactcgc ctgggatttt gtggttactt
    65881 cctgatggta cgcgatctga tgaatcatag tcgtgaaact ggcattcccg ttgggcctgg
    65941 tcgtggttcc tctgccggtt ctctggtggc gtggtgcata ggcataacca acgtcgaccc
    66001 aatccgtcac ggtcttctgt ttgagcgttt catcaaccct gagcgtctcg acttgccgga
    66061 tgcggacttg gacttcagcc aggcacgtcg ccatgaggtg atcgagtatc tgaatgaacg
    66121 ctacggcgaa gattacgttg cgggtattcc gaacttcacc tacctgggcg cagcctctgc
    66181 actacgtgat accgctcgta tttatggtgt ggagtccgca gatatggcgg tatcaaaaga
    66241 actgaagaac gctgaggatg atagccttcc attggaagaa ctgcgcgaac aactggcaag
    66301 tctcgacaaa tacgcaacaa aatatcctga tgcattcaat gcagcctgca agttacaaag
    66361 ccttatgcgt ggctttggta gacatgcggc agggatgatc gtagcaggtg ttcctctgac
    66421 agaacgtaca ccggttgagc gccgtggtga cgcgcgttgt atcgcatttg acaagcgtta
    66481 ctgcgaggct atgggcctaa ttaagctgga cgtgcttggc ctggcaactc tcgatttgct
    66541 cgatagtgca aaacgctaca taaaagagaa cacaggtgaa gatatcaatc ttgatgccat
    66601 ttctcttgaa gatcgcaagg tgctggatgg ttttgctgct gggtacactc aaggtgtttt
    66661 ccagcttgaa tcaggcccaa tgcgcaagct gcttaaagat ttaggtggtg gaattgagcc
    66721 aatgagcttt aaaacggttg ttgctacgac tgcgctcttc cggccgggac caattcagtc
    66781 aggcatgttg gatgattatg tttctgtcgc caaaggcttt atgacgccgg aatcattaca
    66841 ccccgttctt gatgaactta ccgcagaaac aaatggcgtg attctctatc aggaacagac
    66901 gatgaacgcg actcgattgc ttgctggctt cacaatggct gaagctgacg gtgttcgtaa
    66961 agcgatcggc aaaaaggaca tggaaaaaat gaagagcatg ggcgagaagt tcatcgttca
    67021 ggctcaagct ggctggatag acgttgaact ggaagatggc actacgcagc gcattcaccg
    67081 tgcggaacat tttaaatgcg aagacggaac tctgaaaact gtcgaagagg cacttgagca
    67141 cggagcaaca ctacctataa acgcagtacg cgttacagcg tcacatccag ggctatcaga
    67201 gatgaaagcg aaggagatct ggaccgcatt tgagaaaaac ggagcctatc agttcaataa
    67261 atcacactcc gttgcttatt ctttaatcag ttatcagtct atgtggctaa agacgcacta
    67321 ccctgctgag ttcttcgcag ctgcgctcac cattctgggc gaggataagc atcaggggct
    67381 ggtgaaggat gcgctgacct atggcattcg cgtattgcca ccagacgtta atgtgtcatc
    67441 taaccgaatt gagatccgca cactcgaaga cggcagtcag gcactgtatg cgccattctc
    67501 tgctgtgaaa ggctgttctg aaaatggttg tcaggcaatt atgcgtgcac gtgagaaagt
    67561 tggtggcaaa ttcgagtcag tggcacaatt cgatgaagcg gtcgagaagc gtgcatgtaa
    67621 cagtcgtgta cgcgagtcgc ttcataaagt aggggctttt gcgtcaattg agccaggcag
    67681 tctgccagca actgatcctg aacgactgcg cgaccaggct gagctgatgg gcaatctcat
    67741 cattgacgct gttaaagcat cacgtccgtt cgaaatgaat cctaagcgtt ctgccgaaat
    67801 caacgtactc atgacacgta tggcggctga aatgggcttg ggtgaggagt tgatacgccc
    67861 gactattggt attaaaccca aaatcatgat cattctggac aatgcgaacg gcaatgacgc
    67921 tcgtaccggc tacttcatgg agaacggata cgacgacttt aaggcaaaac tactgacagt
    67981 tggagatctg cgcatgggcg atctttatgt cacgggtgtt tgtaagaagg ttaaggacaa
    68041 agagaaagac tataccaaag acgagatagg ccagttcaca gactttatgc gggaagaaat
    68101 taatcttgta cgaccaacct acattttgac gtgtggtagt cgttcaaccg cactatttaa
    68161 caataagagt aaaccatcag atctgattgg tcgtaaggag tacttcccag agcttgatgc
    68221 aaccgtcttc tacggattta acccgaatat cctgtacttc cgaccggaag aaggagagcg
    68281 actggaggcc attctggctg atatcgcgga gacaataaat aagtaataaa gaaaacccgc
    68341 ctgttggcgg gtttataaag aattatggcg cttgttgagg aagtcactcc tcttacgcac
    68401 tttgttttgc catgccggca gttagcttct gcctttgact attcatgcgg caaccccgca
    68461 tttcgccaca atgggcaatt cacttttatg gaataaactg gccgttgtgt cgatttaatt
    68521 agcatggcct taccatgcta atttattcaa cttgtacaat cctacaaatc tatccactca
    68581 ggcttacacc gtcttcgtta ttgtagaaat gaggatcatc agcgttgaat cagcttagag
    68641 caacgacatt tgctgctgct gggcctttag caccattctc gatagagaat tcgacctgct
    68701 ggccttcttc caaagtgcgg aaattattac tctgaattgc cgaaaaatgt acaaaaacat
    68761 ctttactgcc atcagcagga gaaataaagc caaagccttt atcagagtta aaccatttta
    68821 ctaaaccagt cattttatta gacatagata ttaccttctt aattttgtga gccacatagt
    68881 gcggcgagaa tttgatctgt atagattggg acttacttag gcacttaagg aggagactca
    68941 cgaagaaggg aaatcagaag ataacactga actgagactg ctttactaaa actgcttaca
    69001 taaggtctgt cttgcaaacc aacgatgcta ttaacgcata ccccttcttt tcatgcaacc
    69061 tttatttttc aaagataact aatttttgtc accattggtt tagtaaacgt taacgaacca
    69121 gacgatgcca gacaccttta cagagaacag atgcccgcca attggcgggc atcattatgc
    69181 gcatttcgca atatctttgc ggcgtttaat cagtttctcc gcaatttgct ctatttcgtt
    69241 gaaatctttc gagacgctgt ttcgaagcgc cagattccat ttactcaaag ttcgggcatt
    69301 ttggacaatt tgatcgccct ctttaagtcg tccgttattc atgagccatt cagccacatc
    69361 agcccaatcc cagagaggag actggccttt tatgcgttgt acagggcaag ggaagtcgcc
    69421 gcttccgcgc ttaccgtctt tgagcaacgc cactgcctgg cgagacaggt ctgtcagttc
    69481 cgcgatatcg cttaagccca caagagccga gtcgacggat tcaacaattg caccgatacc
    69541 ggctgattca atattgtcga ccgcagatgc gatagctgca tcaagtgatt gtgcttcgcg
    69601 gtcaaattct acatagacgg agtttccata tgcgcaaatt agcgcatcgt cacagccgtt
    69661 ttggtacagc gcgtcttcca gtccttccgt ctcatacgat acgcctgaga gcgtcagagt
    69721 gaagttataa agcgccatag ttttcctttg aagatagttg gacagtttct gcaaaaggcg
    69781 gctaatgccg ccttgaaatc atttacaacg atcaaccatt cgtttgatct gtttggcatg
    69841 gttttcggga ttacccggag tcgaccatac gctcatttgg tgagttttgt gttcaccttc
    69901 tggattaccg catcgcagtc tgcaaaaaca atgtgcagca ccaccagcag ctacccagat
    69961 ccagccttta cttaatgcat agtcaatggc tgcttgaata tgcttattcg gatgttgctt
    70021 cattcgcctc cgataatagt attctattca cagtgttgac atctgtcaac ggcgactgaa
    70081 tttcatccgt tcatgccaca cccccatcca tctcatgtat gtgacctaaa gatggtcagc
    70141 acagcttacc aaaataagac aactaattat cttcggtgat ctcaatatct tcccctgaac
    70201 aaagttgaca tgacgccata aacccgttct ctgtttgata taattgatac agattttata
    70261 agtaggaacc tattagagtg aacactgata ttttttctaa aatcatggcc gatctggagt
    70321 tcgaccgcga caaccttgag gaagtatggc gtaaacagcc acggctttta atggagtatg
    70381 ggtcaaaact agcgcaggca gatcgagatg tcgcagaggc aaaacttaac cttgaagctg
    70441 ttgaagcaaa gctatacgac acagagcgta agaacttgag tatgaacggc attaagttca
    70501 acgagtctgt actggacgct aaggttaaaa caaacccaca gtatctgtct aaacggcaga
    70561 agttggatga agcacggcac atcgcagaca tatacaaaca tgctgtcgcc gccttttcgc
    70621 atcgccgaga catgatcgtt caggcgtcaa agatggccat cgttgaatta gagcgattag
    70681 gctctgaacg ctttattact ccccgttgat ttttgataga taataagtaa gtactgatct
    70741 atcatttaac agctcgaaag agccacgaat gaacgaaagc ccaacgcgca tagcgccatc
    70801 ggccaaatca caacaaggag aaacacatgt ctaagacatt acttgatttg cttaacaaaa
    70861 ctcgtgaaga cattgccgcc aaacgtggta acaacgttga tctgactcgc ttaaaagatg
    70921 gcgtcaacta tatccgtatc ttcccgaata aggacgaccc aaacggtaag ttcttccaga
    70981 ctttcggtat gcactacgtt aagtatcaga acgaggaagg taaagaagca accaacgctt
    71041 atatttgtga gcaacatact cacggtcgcg cttgtcagct atgcgaaatg gtgatggaag
    71101 gtcgcgctcg tcacaagggt aacaaagcaa tggaagaacg catcggtcaa atgcgtgcca
    71161 ctcctcgcta cctggtcaac ggcattcttt ctgctcgtga ggatttcgca gatgctgaga
    71221 aatgccagtt aatcgagctg ccgtctactg tattcgatga tatctgcaaa gcaatcaccg
    71281 aagacatcgc tgatgatatc ggcaatccac tgagcaaaga ggaaggctac gcattcctga
    71341 ttaaacgtac tggctctggt cgcgatacca aatatgacgt ctcgcctaag cgtaaagtct
    71401 acaaaggcga tatcgaagat aaattctgga acacccagca tgatctgatc gcatacgcaa
    71461 atcaggctga tgaaactcgt cttctgtcga cagttcgcac tatgggtcgt ctgattggca
    71521 tcgctgcacc aactgccgca gcatctgcac cagcaatttc ctcaaccgcg aaaacatcgg
    71581 ctgcggcact acctggattt ggctctgtca ctggtcatac agaaggagcg acggctgtag
    71641 caaccgcgca cacaccggct tctgaaccaa ccagtctggt tgatgaagaa atcctccgtg
    71701 ccgttgaaac tgaatttaaa ccagaggcaa gttccgctgc cgttgccgta tcagtcaaag
    71761 agtctgaagc agtcgcagcg acatctgtag caaccgcatc tgcgacggaa gatgaaggtc
    71821 ttgatgacct actgagagag ctggactctc tgtaatccca ttacgtgacc agtaaggcgt
    71881 ctacggacgc cttacttttt ggaaggaatg taccggtgaa ttatctcttc gtagatggca
    71941 atagcctggg ttattaccac caacaatctg acaaattgca caacggcgaa atggaagtac
    72001 aggctgcttt cggctttgtt aagaacgtcc gtcgttatgc ctccatcctc catgcccgac
    72061 ctatgattct ttgggatgga tttagtgaca agcgtcgcga cttttacccg gactacaaag
    72121 caaatcgcga cgacgatcct gatatgaaaa agatgaagga aggctttgct atccagaagc
    72181 catacatcct caaaatgatg accgcgcttg gagttaccca actcattgca aaagatgcag
    72241 aagcggatga tctggccggg ctgctggtat cccgcattgc accgcagcca accgttgaac
    72301 acatctatct gttaacaggc gatagcgact ggcttcagtt agttcgtgaa aacgtaagct
    72361 gggtaagcct gcgcgaagac gccaaaaaca agcaggttaa ttttgagcaa tttgcggagc
    72421 tgacaggatt cgctactcct cgcgcatttt tggaagcaaa agcattacaa ggcgataaat
    72481 cggacaacat tagcggtgtt ggtggcattg gtgctggcgg tgcgaaagag ctgctgcatg
    72541 aatggggaag tgtcgcaacg atggtacgcg gcatcaacga cggctcaatc gtggttgaca
    72601 aaggacgcca taagaccgcc ttcaacaaac tagcgaagaa tgccttcaac gagaaaacag
    72661 gctgtcgaat gctcgaagcg ttcaagagaa acattacgct aatgaacctg attgagacga
    72721 agtttccgcc taccgaaatc gaaacaatca aaggcaatcg tgacgtgaaa gcattcgagc
    72781 aactgtgcta cgagctgaat ttccgttcgt tccttgaaga ccttgaagtg tttgttcttc
    72841 cattcgaaag gtattgctaa tgcttaaatc gattattaat ggcgctacaa ccacccccac
    72901 ccaactggca aaagagattg tcttttatca cggtgagtac gctgtcatcg cactgccgtc
    72961 aattctaggc gctgccggaa tgaaagcgac agatcgcgag tttggattag tcagcgagca
    73021 ggtcgtaaaa atcctcgctc gtgtatccag actccttaat cacgatgcga ttgtattcga
    73081 cgaatccgcc gctttaaaac gaatcaacga aacaaaagga gcctgatcat ggcaaaagga
    73141 aaatccgcac tggcacttgc tctgaaaaag aaaatcggta gcaacgacga aattcagaaa
    73201 gtaactcatt ggattgatac aggctttcca ccgttaaaca aagctatttc tggtcgttac
    73261 gatggcggct tcccatgtgg tcgtatcgtc gaagtattcg gtcctccaag tgcggggaaa
    73321 tgtgttaccg cagacaccat gctgctgacg gagcgtggaa tggtaacagt gaaagagttg
    73381 tttgagattg aagggtacaa agcgacatgc actactcgcg atgtagagca taacgttgga
    73441 ctcatcaatg aaaatggcgt gatagagaag acctcacacc tgacatggaa caaccgtcgc
    73501 aaattcaagc gtattaagct ggcatcaggc ggttatatcg aggctacgtt ccgtcatcca
    73561 atccgtgtgg ttgacgactt aggtaatgtc gtctggcgac atgctgaaaa aatcagtgta
    73621 ggcgacacga ttccttcaat ggttggcaca catcaattcg gcgatcagca cctggatgcc
    73681 aatatcgcaa aactgatggg ctatttaatt gctgacggat acgtggcctc tgaaaattca
    73741 gtgaattttt ctaacacaga tcctttcatc aaggatgagt actaccgcct catttcgctg
    73801 gtatcagaca agatgccagt tacgagaaaa cataacggct cggaagacca tgtgctgttt
    73861 agcaaagagg tgcgttcgct gctttttaaa gaatatggtc tggagtatga gaaagctgct
    73921 ggcaagcagg ttccgttgag tgtgcgtcgc gccaatagcg aggctcaaat tgcattcctt
    73981 cgcggctact ttgagctgga atgccacgtc aatgatggtc gctgcattga ggttgtgagc
    74041 gcgagtgggc tgctgctaca gcaaattcgc ctcatgctcc tgaatctggg gattacgtca
    74101 actatctctg aaaaacacgt cgcgggttat gaaaatacat attaccggct gtcattcagt
    74161 ggctctaatt acgacctttt tctgtcaacg attggtttcg aatctccggc acgtttatca
    74221 gtggcaacca aacgggacat tggttttgac cgcacttact caggctatgt tccgcacatc
    74281 agcggcttag tgaaatcact ctacgagtcg ctcaccaaga ccacccgtga agactacgct
    74341 ctggtagacc acgttattgg ccgcggcgat cgtgtcggaa tagacaaact gcgagaaatc
    74401 tatgtctcct tcattggcag aaagaatcgt tttaacgagc atctgtttgc acaactggca
    74461 gcggtaattg gctctaactt gttctacgac gaagtcgtgg ctattgagga aggtgaagcg
    74521 ccaacgttcg acgtagcgat gccggaaaca cactctttct ggtctaacgg gattatcagc
    74581 cacaacacat tccttgcaac agcagcgatg atctccgctc aaaaacaaga tggtctggcg
    74641 gtattcctcg atcatgaaaa cagttttgac gttggcctgg ctgtagccaa tggcctgaac
    74701 gcagatgagg atgacggtca gtgggtatac aagcagccag ataccttcga agactctgta
    74761 gagttgatcg gcacaatact taaattggtt cgtgatgaag agcttatccc cgaatcagca
    74821 cctatctgta tcgtggctga ctctcttgcg tctatggttc cgaactccaa agccgagaag
    74881 ttcgaaaaga tggctgaagg cactgccaaa gacaaagatc agctaaacat gaacgacaat
    74941 acggcactgg ctcgtgcgac gagtgcgaac ttccctactc tggctttgtg ggcacgcaaa
    75001 tacaacgcct gcatcatctt cttgaatcag gttcgcacaa aaatcggtgt aatgtttggc
    75061 gaccctacta cgtcgccagg tggagattca ccgaagttct acgcttctgt acgtatccgt
    75121 cttggtgcat cggtgatgaa ggatggtaaa gagaagatcg gccaggacgt aggcgcagaa
    75181 tgcatcaaaa acaaagttgc accaccgtat ggcaaatgca cctggaaatt ctacttcgat
    75241 cctactcgtg gcctcgacgt tatcgaatcg ctcgtcgagt acatgctgga agaaggatac
    75301 ctgccaaaga acgccagcgg gcgagttgaa attggtgaca agaaatacac aaaatcgcag
    75361 atcgtcgaga tgtatcggga gaagccatta gcagaaatca ttgctgcgtt acagacgata
    75421 gatgaccgac gagagaagac atcaccaaca gaagaactag aagattaatt atcaggcgtc
    75481 caaacggacg ccttttttgt atattgaaaa tgtataagca cttacttatc attagcacac
    75541 aataaccaca taggaaaaca catgatcaaa ctctatctta tggcagtagt tacaggtcta
    75601 tctgtagttt tcatctactg tttgctggtt ccgtcgctga tctctactaa aagcgacatt
    75661 gccgtaatgc ttggaatcat cgttgctttt ggcgctcctg tgatcgggtt tattgcaggt
    75721 cgtaagtttc taaagtcact catcaattcg aaggagaagt aagtaatgaa gaaaggttta
    75781 ctggcagtga tcgtggccgc tgtttgcaca atgggtctta ctggctgcga tcgcgtggag
    75841 cctggatacg ttggcatcaa agtaaacaaa ttaggtgaag acaaagggat tggtgaagtg
    75901 gttggcgttg gtcgccaatg gacaggtctt aacaccgaac tttacgtatt cccgaccttc
    75961 aaacaaatga agacctacga cgagccgttc acattccaga tgagtgacgg tactgctatt
    76021 ggtcacaaaa ttggcgttgc gtatctggtt aatcgtgaca aggtaacgac ggtgttccag
    76081 acctatcgca aaggcgtaga cgatatcacc gaatcagatc tgcgtcagaa aattgccgac
    76141 tctctaaacc gtttggccag ccgtatgacc actgactcat ttatcgacgg tggtaaggca
    76201 caattgctgg acaacgcact gaaagatatt cagaaagaga tgtctccggt tggtattgag
    76261 gtactgagcc tgtcatgggt tggaaagcct gattacccaa aaaccgtcat agaatctatc
    76321 aacgccaaag taacggctaa ccagcgtact ctgcaacgtc agcaggaagt agaacaacgt
    76381 aaagctgagg cgaatatgct gcgtgaacag gctaatggtg aagctgatgc tatccgtgct
    76441 cgtgcgcagg cagaagcaga tgccattcgt ctgcgcggtg aagctctgcg tcaaaacccg
    76501 aacgttatgg aactggaagc catcaataaa tggaatggtc agttaccgca gtacatgact
    76561 caaggcgcta acactccttt cattacagtg aaataactcc cctaaaaagt tcaggcgtcc
    76621 agttggacgc cttttttatc gcaattatct tattaagaaa acaatttgtt taaaaggata
    76681 agaaaacatg acagctatta agaaactcta cgatgccgca aacgtggctc tggatgttat
    76741 tgatgatgaa gtagcaaaag gctttcctga acctgattgg gcgcatcagc tacgaaacgc
    76801 tatcgcagaa atgaccccac cagatccaac ccccgacgag acagactggc agcgattcat
    76861 ccgtatgtac gctcaggaaa taggcccaac gccaacggca gagcaggcaa tgctgctgaa
    76921 atacttcaaa gaggcgggag aggatttacc aattgatgac tcagcatatt ggtttcactg
    76981 cgcatggcgt aagtatgacg tgatattcac acaaggcatg ggaagcaaag atatggttgt
    77041 gtggcatcta ctccatatag acacagccgt tgacagagtt attgaacagt ttttccctaa
    77101 acaagaagat tgatcgccta ttcataacta acaaaataag taaacactaa ccacaaaagg
    77161 aaaaacacat gagagtttta gttcgaatcg ttaccagcac tgtctatgac gtgtttccgc
    77221 tttttatggt caaagccgat ggccttaacg acgaagaaac tgacgcgctg atccagcgta
    77281 ttctcgttga atatacaggt catgacgctg attcagtgat ggttgatgat gatggtgttt
    77341 gttggcataa cggcaactgt tggtacgtag aagagactca acaaatcagt gatgaagatg
    77401 ccgcacatct tgagcgtatt ttaagcatca gcacttttga gtgagtttac agtaaaattt
    77461 atataagtta gtatctacct atcatgaaga tttttattga atacttgtta ctcatcgttt
    77521 caatagcttt tgtcatcgac tgcattttca ctggtgtcat tcgtaaagtc ttttccccgg
    77581 tgcacgacgt agtcataaac gctttggcta tcgtgctcgt atttaattca gcatttgatg
    77641 taatcaaaga ggtggcagca tgaaggccat cccattcgcg ctgttgttcc tttcttcgat
    77701 cgttgtggcc gacaccactg tttatcagtg tgaaatgtct gtagccgacg ttaagaatgg
    77761 cgctcttacc gacgtcataa aagcaccata tggagcgatg gtcgtagaca gcggcgacca
    77821 gttctatgtt gtgcgtgacg atcgagtgtt gtcatcccca tatctcacaa atcgtaatgg
    77881 caaattaacc ggcgtcggag aagaccactt cgtatacaac aaatacaagg gcttctatgg
    77941 cgttcacgct tctcagcaaa gctacctttt cgatgactgc aaggaggttg gataatggca
    78001 ttaacactgg caggtctgga aatcgagaaa acaagcggct actggcgtgc taagggtttc
    78061 aagcagcctg gcattcttga gcgtctggaa cgtgaagatg ggtatatcgt ccaccagcgg
    78121 cgtgaatggc gtatgtacga tccagaaaca ggaaaactga ctacaaaagc cggaacactt
    78181 tggggtctgt tgaagaaaat acactaatag caccaaccac tgcggtgagt agtctgctca
    78241 ccgcattcgt atccagctca ttatccactg tagtcagtaa caaaaccttc ctacatatct
    78301 gctaaccact gtagcgagta gacagccttt cattcgcatc aaacaaccac tgtggtgagt
    78361 attggagtga gatgtccagc gggtatccac tatggtgagt aagcctttgc tgttttcagt
    78421 ggatatccac tcgccacagt ggatacgctc aaagatgagg gcgacaacaa ccactataga
    78481 cagtaaattt tccaagatac ccagtgacca ctaacctcgc agcccttgtt tcatcagtgt
    78541 tagtgatcac taacatttaa ttcagtattt gcattctact gcctacagtg gttatcaaca
    78601 gataacgata ctcactatag tggatagcga attacagata taagtaaggc tcactacagt
    78661 ggatagtgag ccttactact aactacagtg gttgggctat ttgcgagtct ttgccttgcg
    78721 caactcttcg agaagcgcca gttcttcttt actcaacatg accattttgc cttctttttc
    78781 gtcaggcaca acgtcgataa tgtcatcttg attctcatct gggaggctat attccgtctc
    78841 atcattatcg atttttgttg atatttgtgc ctggcgtaac tttggccgcc tatagtggat
    78901 gacgaagtag accgagtttc cgcgcttaac ttccgtgtaa tcgagatatc ctatctctcg
    78961 cagctgttcc atagcctttc ttaccgtcgc attctgagta atagtgcgac ttgtcagatt
    79021 aagtctggcg cgtaaacggg ctagtgatat tggtgccgga tcaggcggca aactttcaat
    79081 aaaagtataa agcgcctgag cggattcttt tcttgagagt tcgtttatag cccggagttg
    79141 cagaagaacc tttttgtcga actggtagag ttcgaaaatc ttgggatctg ctttcagttc
    79201 gaccgtatcg tgcttgacac tatattttgc cgtttgcaca agatgcgtta cgtaaaactc
    79261 atcagagcct ttgctacgga acgagatggt atttgtggca atacggctta gcgaactgtc
    79321 caggcgtttg cgtaacttcg ctgatgacct ggcggtcgga atgccacaca gccttacaaa
    79381 ctcaacgaac ggcaacgtga cagtatcgcc aacgaccttg tgtttggcaa acgcgtggat
    79441 tattcccacc cacgttttaa agtcgttatc catatcaagc cgaaggccag agatccttat
    79501 gtcttcgtac ccttcggctt ttgccagaga cagctgtttg agttcagcag aggcatccat
    79561 agagaccatt tgcccctttc ttcctctgga tgtcgatttc agcgtcggaa cgaagaggcc
    79621 cagacgcatc aaagcaacag gctgaacagt gttattagtg ttaggaacca acgtaacaac
    79681 ttcgcctgtc tttttatctg tttctgaaaa tgcttcaacg atcgctatgt ttttattgcc
    79741 gttttcgctc attccaaatg tctcttgtta ttcgacggct ttggtggcct ttgctgatta
    79801 cagtggatag tagcactcat cacagcggtt atcctaccgt ctatagtggt ttttctcctc
    79861 tctatagtgg ttgctttgct ccctatagtg gattattcgc tctctatagt ggatatcgat
    79921 catgtctgaa ggcagatggc acaacggttt gaggtgggcg gggatcttat tgggtcttta
    79981 tgggtctctt tggttctctt tgtgatctga attactggat cgggcctgtg gataaaaatc
    80041 agaggatttc aagaacaggt aactctactc ttctgtggat atttaaggac ataagaatca
    80101 tgactaagag tagagtttaa aatatatcta tgtatatcag tatattaatg aagaattaag
    80161 tcattaagtt atccataaaa gacaatgtgc tctactctct acagtggata tatacagctc
    80221 tccagagtgg ttgttttact cttcatagcg gttattcagc tctctacaga ggtttctttg
    80281 ctctccatag tggatggcaa caccctttca gaccagtaac cgcaacggct agaggcgatc
    80341 ggggatctct ttggatcttc tatttgatct tacttgggat ctcttatttg gatctatcca
    80401 gtggataact gggataagta aaacaggcaa atgcagatta aggtatgcct aatgagttat
    80461 cgtagcatcg gccaataact actgaaaaaa cgattatgga tctaaaacgc acgcgctggg
    80521 ttcgtcgtct tgaggacggc tcctacacta tcgaatcaaa ttccaacctg aataagcaga
    80581 agttactttg tgatatctgc ggtatagcgg cgaagtgccc gatctacgaa accagaatta
    80641 aacttgataa ggctggtgtg aactttcatt tgaatagttg tatcaggtac gttccattgc
    80701 tcgcatttcg taaaccgatc atcggattgg atgcgcccta cttcaacaca ctccgttcag
    80761 gtgtgacgtg gcgagatcgt ttatcaccag acaagctgat ttgcctcgta tccgcagaca
    80821 cagggaaaat catccgtttt gggaaagtag acaaggttta ctcaggccca gtagacgaaa
    80881 tgttgcggaa acacagccgg tttaatcatc tctgtatggg tggtgagaaa atcgagaagg
    80941 tagaagaagt gatccgcaaa tcctacggac actttctgac caaagatagc ctgctcactg
    81001 caatctacat cagacatgta aaacgtgagt tcgacctcga ataccacagc gaagaagagc
    81061 ttaaccttgt tgacccacgt ccaaaagctg gcgtcataag cataaacgca gcgcgtaaaa
    81121 agcccactga cgcgctgtaa ccctccagat cgtatattgg cgtagataga atctacgccc
    81181 cctcaaaata gctctcatag cgttctacag tgatcctgtc ttatttttag tcatacagac
    81241 aagcaaagtt gcgccacgat aaataggtat atacttactt ataaattttg tatattaagg
    81301 cgctcgtttc attcctaaca taccgttatg catagttgtt taccttctca ttgctcttag
    81361 aatttgtatc aaaataacca caaaggaaaa acacatgact ttgccatacg gcgtcatttc
    81421 tgactgccac taccacaaat gggatgcgtt ctccacgacg aacgctgagg ggcttaactc
    81481 cagacttgaa atacagttgg aagcaacgaa agaagcagcc atcgccatga agaaggccgg
    81541 ttgtaagtac atgttggttg ccggtgatac atttcacgtc cgaggaactg tgtccccttc
    81601 tgttttgcat tacgtaactg aaacgtacaa gtggattatc aacgagcttg atctgacagt
    81661 agtaatgctg gccggtaatc acgatcttga aaccaacgat tcagtatata gcgccaacgc
    81721 agcagcatcg ctgagttcta tcggcgtggt aatcgtatgt ggcaagcgcc cacactcaat
    81781 aaaaattggt gatgtgactg tccacctgat tagctggcgt aacaatcatg cggagcttat
    81841 cagcgatctg aaagcattac gtaagagcgt agaaggtgat aatcatgacg ttgttatcca
    81901 tacatccatt aacaaagcca ttccaacaat gcctgacgtc ggtatcgatg cgcaggagtt
    81961 aaaggatatc ggctttcgtc tcgtgcttag tgggcattac cacaaccaca aagaggtcat
    82021 tcctggagtt atcagtgtcg gtgcactgac tcatcaaaat tggggagatg ttggatctct
    82081 ggctggctac atgatcgtaa acccggacgg cagtttcagt cactacgaaa ccagtgcgcc
    82141 taaattcatt aacctggaag atgatgttgc tgatgaccaa attcgcggca actacgtgcg
    82201 tttccgcgcc gtaattgaga acgatgaaga aggcattaag taccagaaca tcctcaaaac
    82261 aatgggtgca aaaggtgtcg tgtgcaactt catccgtaag tcatcaatga tggaaggtac
    82321 agccagcaca actgaaacca gcaaaatcga tagcctggga gagtcggtat ctgcttattg
    82381 caagattgtc cacgatactg acggcggatt tgatctgagc aaattggata ttttgtgtca
    82441 ggaaatcctc accgaagcgg agagttcgga ggctgtgtga agcaaagtcg ttatgggagc
    82501 tttcgagact ttgccatcac gatgaaaaga cttgaacgag gccagacggt gatgtttcac
    82561 aagccctacc cgccacaagg aaatcccgta gcgttttatc ttggaaggtt aacaagaaaa
    82621 ggcgtattga ggcgcagatc cttcccggcg catacggagt tcagattgaa agaaggccaa
    82681 aagctaacac acggtatcag aggtgttata tgaagttttt aaagctccag gttgagaatt
    82741 ttatggctat cgccagcgcg gaggtcgagt tagatcagcg tggtttagtg ctcattcagg
    82801 gtgttaatag tgatgatagt tccgcatcaa gtaatggctc tggaaagtca actctaatga
    82861 atagcctgat gtggtgtctt tatggcgaaa cagctcatgg tgtgaagggt gacgatgtgt
    82921 tgtctaccga ccatgaaaag aactgtcgtg ttgcagtaac catcgaggat gaaggcaaga
    82981 gatacgcaat cattcgtcac cgtaaacaca aagagttcaa aaatcgtctt atcgttcgtg
    83041 gtgaagatgg cgatatgacg aaaggcaaag atgcgctgac gcaggagttc gtcgagcgtc
    83101 tgatcggtgc atctaaagag gttttcatgg cttccatcta tgcgagccaa gaagctatgc
    83161 cagatttacc tggaatgtcc gacaaaaacc tcaaaaccat cgtagaagaa gccgctggcg
    83221 ttgacagact gacacgcgcc tacgctattg ctcgtgagcg agctaatgca gctgccgcac
    83281 gtatggatgt ggttaaaacc aaattggagt cgacaatctc gaccattgag gcaacacagt
    83341 cagaaattga gtcagcgaaa gcctcctctg aatcatggga gcaagagcgt tctaaacgtt
    83401 atgacgatgc cctggctggg ctggccagtg ccgaagttga gttaacggaa gttgaacttg
    83461 agatccgcac tcttcccgaa cagatacgtg ataccgagaa ggcaatcgaa agtgagcgca
    83521 aaaagttagc ctcaaaagaa gaacatgacg ccaagttgct caaagttcgt ggtgcgataa
    83581 ctgatattcg ggcaagcatc aaagctacag aaaatagtca ggctgatgca atgaaccgcg
    83641 cgcgtaattt taagaccaaa gcagaagagg ttggtactaa agtgggatca ccatgcccta
    83701 cttgtggcaa agcctactgc gaagaagatc tatcaacggt gaaggagagt ttcattgaac
    83761 aagcacgtca ggaaattggt caggcgaaga cacttgcaga ggcaatggct aaacacaaaa
    83821 cgaatcttga gaaagcgtta agcattgagt ctgcccttgt taaaacgaca cctgatgtaa
    83881 cggctatcat tgcccggatt gaagagctta cgaaacaact ctcatctttg cgtcatcgtg
    83941 agaaggaggt tgttgctatt gagtctcttg tgactcgtgc tcgtactgag gtcgatcgta
    84001 tatcaaaaga gattaatccg tttattgctc ttatcgccag acacgaagat aacctggtat
    84061 ccagtaagtc taccttcaag tccttaaaag atgagttgaa ggctattcag gaacaaacgt
    84121 tgctattgga aaaagctcgt caggtctact ctcctgccgg ggtgcgttct catattttga
    84181 cgtctgttac gcctttcctg aatacacgca ctgccgagta tctcaatacg ttgtctgacg
    84241 ggaatattac tgctgagtgg tcgacgatgg atgtcactaa aaaaggtgag tatcgcgaca
    84301 aattcaacat tagtgtgcag aagaaaggtt caagtaagtc gttccagacc ctctctggtg
    84361 gtgagaagcg gaaggttcgc attgcgtgtt ctttggcatt gcaggatctg gttagtaacc
    84421 gggcgagtaa aaacatcgat ttgtttatcg gcgacgaaat tgacgatgca ctcgatacag
    84481 ccggtcttga acgcctcatg ggtattctgg agtccaaagc tcgcgagcga ggtactgtgc
    84541 tgattatctc ccataaagag atgaagtcat ggttccggga aactattacg ctggaagtta
    84601 aagaggggcg cagctatgtc gtttaaatta agccgctcgc agtttttgca ggtatttgca
    84661 gtgatgcagt cgataaaact gatcaatggg catacttcca atggtgcggc tccacgtatt
    84721 ctgtggggaa gcaacaatat tgacggagta caattcgccg cgttgcttgg tctaatatcc
    84781 gagacgccat tgatgcaaag tttgaaatca ctaccacctg gatgtattgc gccgatcctg
    84841 attaatcctt ttgttgaggg gggatatctt cccaacgtcg ggcctgggtt tattgcatcc
    84901 catgaaactg aagatcttaa cattaatagc gaagggttct ttgggggaat gggtgcgcat
    84961 cactgtatgg ctttcacgaa ccttattcga cttgccaata agcgggtgga tagtttggca
    85021 tcgccaggtg atgcttttac tggtttcctt atccaaagga gggataaaaa gtacagtgcg
    85081 gacaaactac agtttgttgg taagtatgga gaaatggtag aaatcgaact tcagctccct
    85141 catgttttag caaacgatag tgcagacagt cggaggctgt tgggcatcat gcgtcatttc
    85201 atagcaagtg gcgttaaaca tgccgtagat aaacgtgtca cgcaggaaaa tgagtattca
    85261 gactttgcaa actatcccca accaacgttg caaacggcaa tagtagccaa ttcgttggag
    85321 gcgagattat tggaaaaccc tatatgggga acatggtaag gagactatat gagtaaaaaa
    85381 atcagcgtag ttggtgttga tccctcaatg agcaactttg ggcttgctgt gggcacttta
    85441 gaccttgaga cggacgaact tgagattcac ggccttactc ttgttgagac taaagcgggg
    85501 agtaacaaaa agaccgttcg tgtgaacagt gacgatctgc gccgtgccag tgaaatatgg
    85561 cgtgttgcga agccaatcat tgataaggca aatatggttt tttgtgagct accggttggg
    85621 agccaaaact ctcgttcgca gacgtcttac ggtatttgca tcggtgtact tgcgtgcgta
    85681 gataagccat tgatccaggt tactccaaac gaaatcaagc attttgttgg caataaactt
    85741 actacatcga aagaagagat tatccagtgg gctacgaaaa aacaccctaa agcaccgtgg
    85801 ctgcgtcgta agcaatctgg acaggatgtt ctcgtgaaca aaaacgaaca tttggctgat
    85861 gcggtggctg ccatccatac cggtatgcaa acagatcagt ttcgccaggt gcgcgatgtt
    85921 ctcaagtctc tcatttgatc tcattgatag gtaagtgctt atctattaac atgggccact
    85981 aaatatagtg gccctcttta tttggtgata catgataagc atcgtaaaac gtaacggcca
    86041 aacagagccg ttatccgaag aaaaatacaa ccgcgtcgta atgtatggcg tagaaggcat
    86101 tcgtggtgta agcgcatccg ctgtagcaat gggagctgcg gccagcattt ttgatgggat
    86161 taccaccagc cagttgcatg aggctttggt taaatctgcc gctgatttga tctcaccaga
    86221 tgcaccaaat tactcgcagg tggctgcccg cctgaacatt tttaaaatcc gcaaagatgc
    86281 cttcggtcgt tacgactatc cgaacttcta ccaacacatt gtcaagaacg ttaacaaggg
    86341 cgtttatgac aaggatttgc tgacacatta ttcatttgaa gagatcgaag aactcggcaa
    86401 ttacattaag cctaaacgtg acgatctttt tggctatgca gctacggtgc agttgcaaag
    86461 caaatacctc gttcaaaacc gtgttactgg tgagattcac gaaggcccgc aacatatcta
    86521 tatgctggtg ggcatgtgtc tgttccagaa ttgggaagac gactgcgcgg gcaaaacacg
    86581 tatggagatg gtcaaaggtt tctatgacgt tacaagtacg ttcaaactgt ctctgcccac
    86641 accaatcatg gctggcgtcc gtactccaac ccgtcagttc tccagttgtg tgctgattga
    86701 gtctggcgat agtctgaaag gtattagtgc agcttcagcc gcaattatcg actacgtttc
    86761 acgtcgagct ggaattggta ttggttttgg ccgtatccgt gcgctgggca gtgagatccg
    86821 caatggtgaa gccacccata ccggagttat tccattcctg aagcatttcc agactgctgt
    86881 taaatcttgc tcgcaaggtg gtgtccgtgg tggcgcagca acagcgtttt acccgatttg
    86941 gcatcttgaa gttgaaagtc tgctggtggt gaaaaataac cgtggtattg atgaaaaccg
    87001 agttcgccat cttgattacg gcgtcatgag taaccgtcta atgtaccgtc gactcgtcag
    87061 aagcgagaac atcactctgt tcagcccgca tgatgtgcct gatatgtacg aagccttctt
    87121 cacagaccag gatctgtttg aaaagctgta ccataaatac gaagccgatg attcaattcg
    87181 caagaagtca gtacctgcca ttgagctgtt ctcatctctg atgcaggaac gagcgtccac
    87241 gggccgaatt tatattgcga acgtcgatca tattaacgag catggcgctt tcattcctgc
    87301 tcttgcacct gttcgccagt caaacctgtg catggagatc actctaccca ctcgtccact
    87361 ggcatttacc gacgacccga acggtgagat cgcgctatgc actttatccg cttttaacct
    87421 cggagccatc cgttcactgg agtctcttaa agaggtggcg ttctatgccg ttgctgcact
    87481 ggattcgtta ctggattacc aagactatcc gatggaggca gccgaagtgc ctgccaaagc
    87541 tcgtcgtagc ttgggaattg gtgtaaccaa ctttgcttat tacctggcaa agaatggcgt
    87601 tcgttattct gataccgctg gcaataaact ggtgcatgaa acgttcgaag ctatccagta
    87661 ttaccttctt gatgccagct gccgacttgc tgaagcaaaa ggtgagtgtg actggtttga
    87721 gcagaccaag tacgcaattg gtcagttgcc gatcgaccat taccgttctt cattagacga
    87781 aagtggcgaa accaactttg agttaaagat gccgtgggaa gaactgcgtg aacgtattgc
    87841 aaaatacggc cttcgcaact ccacactgac ggcacaaatg ccatgcgaga cttccagcca
    87901 gatcactaat tccaccaacg gcatcgagcc gcctcgtggc ccggtgtcgg tgaaatcttc
    87961 taaggacggc atcgttaaga tggtcgtgcc tgagtttgaa aaactgaagg aacagtatga
    88021 atacctgtgg gatatgccgg acaaccgcgg ctatctgaca aaggtggcga tcatccagaa
    88081 gttctttgac caggctattt cagccaatac caactatgac ccttctcgct ttgaaggcga
    88141 taaagtccca atgatgacgc tactgtcaga tttgcttctc gcctacaaga tgggagttaa
    88201 aacgctttac taccacaaca ccagagatgg tgcaggaaag cgtgatgacg acgaagcgca
    88261 gaatccactg acgcaagctg tagccgtcga gccagaagat gagtgcgacg gagcctgcaa
    88321 aatctgacat atggtggggt atatccccac cttatctttg atttgtaagc cttgtttaaa
    88381 cacataagat aacaacttgt ttaaacgcac caaaaaagaa aaaggaaaaa cacatgtcat
    88441 attcaacgtt ccgtttgggt gctaatgatg caaccaaaga gcctatgttc ctcggacaat
    88501 ctgtcaacgt ggcacgttac gatcagcaaa aataccgtga ttttgaaaag ttgattgaac
    88561 gtcaattgtc tttcttctgg cggccggaag aagttgatat ttcgagcgat cgtatcgact
    88621 tcaacacgaa gctgcgggac cacgaacgtc acatttttct gagcaatctc cgttatcaaa
    88681 cgttactcga ttcagttcag ggacgtagtc caaatgcaac gctgctgccg cttatctcta
    88741 ttcctgaact ggaaacgtgg gttgaaacat ggtctttctc tgagactatc catagccgca
    88801 gctacaccca cattattcgt ggcatggtgg acgatccgag cattgttttt gacggtattg
    88861 ttacggatga agaaatcatc aaccgagcga tcagtatctc tgctgaatat gacaggcttt
    88921 atgggatgac ctgcgagcgc cagtcgttag gtgagaagga atttgagcgt ctgtacgtaa
    88981 atgaatatgg ctgggagcca taccctttgc accgtcagct tttccgcacg ttggtgtcca
    89041 ttaatgcgct tgaggcgatc cgtttctatg taagttttgc atgtacgttt gcctttggcg
    89101 aacggaagtt gcttgagggt aacaccaaaa ttatgcgctt tattgcccgt gatgaagctc
    89161 tgcattgcga aggaactgaa cgcatgatcc gcttcatgcg taccggtcgc gaaggtttat
    89221 tgtggaaaga gattgctgct gatgaagaaa acgtcattta cgacaccatg aaatcagtcg
    89281 ccgaacaaga aatgaactgg gcagactatc tcttcaaaga cggttcgatg attggtttaa
    89341 acgcggatat tctgaagacc tatgtaaaat accgaaccaa tctggctatg aatcgtcttg
    89401 gcctgaaggc tttatttcca gaagttacca ctgatccgct ggtctggatg aacaagtggt
    89461 tgttaaccga cacactgcaa attgcaccac aagaggcaga gcaaagcaca tatctggtag
    89521 gtcagatcga ttctaccgtg gacaaggctt ctctaagcca gtttgcagac ctgtaaaccg
    89581 atacaaagca ttatgtggcc tggcaacgct gggccacaat ggatcacaag aattaagaag
    89641 gaacaaaact agcatgaact ttaccaaact gactgaccac ctgaaacttg ccaccgatcg
    89701 actcattgga tttaagccag aaccatatga gttgcatgaa ggccatggtg tagccactga
    89761 aagtatttac aagatggtcg atcagtttca tgaactcttc cagcatccga gacgcgttat
    89821 gccgacacca gagctgcttc gtctccgtgc aagcctgatt catgaagaag ctgtagtgga
    89881 aggtattcca gccgcaatga atggggatat tgagcaactg ctggatgcaa tggccgactt
    89941 tttatacgtt ggtgttggta cgatggtctc catcaaaggt ggtatttcta ccggcatgac
    90001 ctattacacg caggaacaga gcattgatcg ctttatgcag acaatttttg tgcccggtaa
    90061 cactgttttc gatgatatgg caatgccatt tcaggaagct cgtgaggcgt catgtatgct
    90121 cgaagagctg gcagataaac ttgagaagaa gactgttaag gattctgagc tgattcagga
    90181 actgcgccgt gtaatgaaca aaatctatgt ggcgtgcatg atgacttatc gactggctga
    90241 tttcctcggt atcaatgtcg tcgagctggt tggcgaaatt catcggtcta acatgacaaa
    90301 attatggcct gctgatgttg aggaacgtcg ccaggctgtg gccaactgca aatacgactc
    90361 ttcagatctg ggattccgcc atgctgatgg caccgataag atgatcggtt ttcgaatttc
    90421 cgatggaaag attctgaagt ctccaaccta tagtgatgtc gatttatcct cctttgttga
    90481 gcaagctaaa gcctcagcaa tgtacggaat gatcaaaaaa taattgtagg tagttatcta
    90541 tctgtgtata tttaacatgc gtaataaaac tctggaatga ctattcgttt tggtggccta
    90601 tggccaccat ttttttatct gtctggtctt gttctctcaa tgaatgtaaa ctcacacgat
    90661 gaataagtaa ttacttatct ttgtgaggtt tttgtgtcac tccttttgaa tcgagagcat
    90721 acgaacggtc aggtaacaaa cgcatcgtat gcaaaagtta ttgagacggt gcttaaaagc
    90781 ggcgtgcagg ctgatgatcg cacaggcact ggtactttaa gcacctgcta cgttccctct
    90841 tattacatgc ttactggtgg gactgtgccg cttatttctg gaaaggcggt aaatcttaag
    90901 ccactgcttg tcgaacttga gtggtatctg aaaggcacgg gcaacatcca atttctcaag
    90961 gataacggcg ttaagatttg ggatgcatgg gccgatgaga atggcgattt ggggccggtt
    91021 tacggtaagc agtggcgtcg atgggaagat acccgcatcg tgagccatag tgaatatctg
    91081 agcaagatcg ctactttccg tgaacgcggg tacaaagtcg agggatacct gggtatcagt
    91141 gaagatcgcg tagtgctgtc ccgtgaaatc gatcagctac agcgtattgt cgacacactg
    91201 cgcacgaacc ctaccgatcg tcgcatcatg cttaacgcat ggaacgtagg cgagcttgag
    91261 gatatgaaac tgccaccttg ccactttgtc ttctctttgt ggagtcgtga gctggatttt
    91321 gaaacccgtt taacgatggc aactgacatt ggtatacagc acaaccgcca cggccacgag
    91381 tccatttaca cccagatgct gtgtcttctg gaaagagatg gcggcattac tgagaacatg
    91441 ctggacgagc tgggcatccc gaaacgcatt ctgaactcat gcctggttca acgcagtgtc
    91501 gatacctttg ttggtatgcc gttcaacatc gccggttacg gcattctcac ccacttcctc
    91561 gcgaagatca ccggccacat ggctggcgca ttcgtgcatt ttggctttga cgtgcatttg
    91621 tacaacaacc acatggaagg cgttgaggaa ctgatgaagc gcgagccgcc caaagagtcc
    91681 gacccggttg tcatcttccc gcatgaatgg gccgaactgg acgacttcaa atgggaaggt
    91741 gtgcaggtgt gcggctacag ccctcttccg tggatcaagg ttccagtggc ggtgtgatat
    91801 ggcaagaggt atgtatgtct tatgcgaaat tgaaggtgtg ctggcaaatg ccagccatcg
    91861 taaatcagta tctgacgcgg atgcaggcca gctcattgcc ggtgatgaac tcattttccc
    91921 caccagccgt atgttgcgtg gttttgctcg ctcaggggct gaagtggtgc ttatcagtag
    91981 ccgctctgaa actcttgaag cgccaactaa acgatggctg aaagattttg gcgttgatta
    92041 tgactggctt catctcgtac cgaatggcac cagttatgag aagcatatta agcgcacatt
    92101 agcggagcat aaaggcgatc tgcttatcgc tgcgctggtg cacgatcctc gactccgtgc
    92161 cgctttagct gactctcatc atagaccggt catctatgag gtgagcaaat gaagatgata
    92221 gctgctgttg gccgtaacta tgagatcggc atagcgaacg aactgccctg gcgttgttct
    92281 accgatctga agctatttaa gagactcacc aaaaacgcca ctgtcgttat ggggcgtaaa
    92341 acgatggaaa gtctcaaacg ccctcttcca gagcgtcata acctcgtttt gacgcgctct
    92401 catggctttg taccaaatgg attctaccct gctggtgtgg atgatgtgtt gcgattacca
    92461 gagcctgtgt gggtgattgg cggggaacaa atttactcgc tattcatgcc gcatgttgaa
    92521 gagatttggc tctcccacat cggcgttgat gtaccaaacg ccgatgcatt cttcccggca
    92581 agcatgatgc gtaacttagg ctttgtgcct gttgaaacag cttataccca acgagccagc
    92641 gaggaagagc ctggcttttc gcagatcgta tacagaaggt cgtaatggat taccggattg
    92701 ggatcactgg tgctcagggc agtgggaaaa caaccctggc aaaatatatc gacaaacatt
    92761 acggaatccc ttacgtggat gctggtgtcg gaagtttgat gagccgcctc ggtgttcgag
    92821 taggtgagtc tatgcctcta tatgagcggc ttcagattca aatggaaata gcaaagcata
    92881 tagagctact tacgcgtggt gttgaaggct ttgttatcga tcgcacacct gctgatgtta
    92941 tggcctacac gttggatttg gtcggccata ccaatgaaga tcggtgtatt gagttagccc
    93001 tcgatatcga aaagttttgc cacaaaactg ctatttcaaa ctttaacgcc attgctggcc
    93061 tacgcccggg ggtcgctctc tcagagcgag attacttgcg atcacaacga gcatcattag
    93121 accgtctgta tgtcgctcgt attgatgcgt tgatgtgcgg ggaactgaca aaaattcacc
    93181 tgcatccgca aaggggagat ctgcaaacct tcgtcgtttc caaccgatat cgcacggttg
    93241 aagcaagagc cagatcagtg atgagaatgc tagataacgc tgtagaaaag atagaaaacc
    93301 ggttctgtgg ccgagtgacc gttcattaga aattgttcgc ctcttcgaca ttgcgacaat
    93361 aaaactctca aaatgggtta aggataaaaa atgtttagtg aaatgttact tgaagatgaa
    93421 ctggatcgga aaacaacaga ggctttgatt cgtgtagcgg acgaacattc ccggtcgctt
    93481 atgagcgatc gagaggctcg tctggctatt cgtaccgtat tcgaaattgc gcaagggctt
    93541 gttggcgcac aagtaggtga agccattaac atcgccatgt ctcagttcag tgaagacagt
    93601 aaaaagcctc tgtttcctat gcatttgatg ctggctggtg gcacggtgct ttatatctct
    93661 gtttgtctgg atagcaacca aatcaatatt ctcaacactg cgtcaggtaa gtggaaagat
    93721 ccgattgtct gtgaaaccag tgaagaaact ttgaaaaaag cggctcaatt tgtacgtagc
    93781 gcactactta agggcgctaa gaagttgtaa ggagttctga tgacaacgat tgttgcaggc
    93841 atcgatatcg agtctacggg actggatttc cttgctggtc ataaaattat tgaaatcgca
    93901 attacccgct atgaactgga gacacagaca atggttgata gttttgcaat gcggttcaac
    93961 ccgcgtcgca gtatcgatcc gaaagcgcaa gcggtacacg gcatcagtct tgaagattta
    94021 gccgccgagc cgttactagc cgatcatgct tcatctgtcg cttcgtatct ctcttcttcc
    94081 gatatttgga tagcccacaa cggcgaagcg ttcgatattc cgtttattag gcatgagctg
    94141 aaaagttacg gtttttcgct tccagacatc ccattgattg acactctatt ttccctgtgg
    94201 gccacagaag acggtaaacg tccccgcctt gaagagttgg ccttctctct tggctttata
    94261 tacgatcatg ccaaagcaca tagtgcctta tatgacacaa acttaatgat gcaatgcttc
    94321 tttaaggcac gtaataagta cggatttttt aaattaccct ctgaaattgt gtaaaacaaa
    94381 agcctacttt aaagagagtt taaagtaggc tttcttttaa agaacagtcg cctttcaatc
    94441 atttcctgcc tgtatttaat acttttccgc ctgataggtt tagtcaaaat gtagccatcg
    94501 aaacgcaaat gtaaccaagc agaaggagac ttacatgagt tcggttgaaa atgtaatgac
    94561 aaatgatgat cttgacgagc tgacagccat gttgcaatca cttgatgagc cagtaaaaaa
    94621 agccgcacag gttgaaaata ctgatgatat tgacgatctg ctgctcggcc tcgatgctgg
    94681 cgtagccatg agttctgatg atgttgccga agaactgttc aatgaagaaa aagcaggtga
    94741 tttcagctct gctttaaatg agttggagtt agcgcatgag cctataaacg taattaacgc
    94801 tgaaagtgtt gaatctgccg aaaacgagcc agaacaattg ggattgattg aggttgaaga
    94861 gtgtgttgat gttaatgatg aattaaaagt tcaacagtca aatgatagca acacaaataa
    94921 aaaagcgcgt actgcaagag gtcctcgttt tactttaagt gataaagatg attcgttttt
    94981 caataaagca ggcttagaaa cagatatttt cttagacgct tacgaaaacg cgcctgtcaa
    95041 agcaaaggat aagatattaa accttcttaa ttggtttagc ggaggtccag atattagtgt
    95101 ttacacggta atttccatga gacaccttct cacagaaaag aaggctacaa gtaatagtat
    95161 taagattgct ttaatgagca atccagaaaa accgtatccg cttaacactg cgtcaactca
    95221 ggctgggcaa atgatggctg tatttccagc gacaggaatt gccgttagag acggtggaaa
    95281 tctaacattg aacgaagaat caccgatcgt taagaagttt gtagcggagt acactattgg
    95341 atgacgtccc cctactgaaa ataaagccca tagagagctt tatagtgctg ggtaagccaa
    95401 tcacatatcc agcaccacaa aaaagcgcca gagagcttct cgtttgcatt tctggcgcgt
    95461 tttatttgat tgccagacat aaaatcaaat gcaaaaatag gtaatcactt acctatcgag
    95521 aaagacgatg attgcagccg aaaaaatcaa acagcgaaag cgcgacaact ctcttcgtga
    95581 cctctggaga acacctgact ggctgttttc tgccattcaa cgttatcttg gagtgacatt
    95641 tgatgttgac gttgcctgca acaaggacaa tgcgaagctg cctaatttca taggcgttga
    95701 gcgtgatgct ttgaaatctg aatggggaca gccaggtaca attgccttcc tcaatccacc
    95761 ctactccaaa atctccccct ggattgatgc ggctatacgt gagcaggctc gcggagttac
    95821 aacagtgatg ctaattcctc aatccctcga tacaaagtgg tatgagcgtg caacagagtg
    95881 tgcgaatgag acgattattc tgtctggtgg ccgcgtagcg tttgtcgagc ctgacgtcaa
    95941 tttgggtcag gtagaagtaa acatcaaccc cggtggcagt atgctcgttg tttttcgagg
    96001 attctgtcag gacgctgggc actctataag caagatccct ttggacgtca tgaaaagtct
    96061 gggagggtat gatcctgcga atgtgatcag gaaaaaaaga caatcaaaga aggctgctta
    96121 gtttgttctg gcgtctgtaa ttagcctgct tctgtatata taaataacta catattaatt
    96181 attaatatac ggaagcaggc tgttttatat cagagactcc cagacctgaa catcactaca
    96241 gaatccacta gaaccccttc ccagacgctt taaaatcgat tttatgaacc actttaagga
    96301 aaccaacatg tcatacccga ctaatgtcgt tgcgctcgta gagagcgatt ttctggccca
    96361 ggctcgtgaa atgatgaaag atcgtgagca ggctttcaac ttgtacgagt gggcaattaa
    96421 gtgcttgcat cttggcgagc atcgcgaact tgttgaacag cttttaggtg agttgatcaa
    96481 tgaggtgttt gccttgaatg ttcaactaca tggtcgagaa aataatcaat cacaatgata
    96541 gataagtaca aactatttat aaagtgaatt gtaagtgcta agatctgata gtttccagtc
    96601 gtagactgga gactcgacct gatgggtggg ggtaagcgtc actggcgtca ggtttaaaaa
    96661 agctcactac cagcgtagaa ccggcaccgt ttaggggttg gggaaggggg aaccaaagtg
    96721 ggcagagaca agggtcactt tatgattgtc gagtctgggg tgtttcgaga ggttgaatcc
    96781 agtactcccc ttcataaagt gtgggaagat ctcggttctg gggtgctgtc atccataact
    96841 tcccaagcct aagctggcag tagacttagg tcataacttt tcaggttatg aaacgaccag
    96901 gttggtgagg aaattttgta ctcacctccc tgggagagta ttacctgaaa agacaacctc
    96961 tcacttcgtt cgaggtgaac ttcactcact tcgttcgttc agttcaggtt tataaaaacc
    97021 tgttctggga agtaatttgt ttattttaat aattattaac acgcacgcgt gtgcgcacgc
    97081 gcgaggaaaa aatcggcgcg gcgcttgatt caggagttta tatgacgacg aagacaccag
    97141 cccgatcgca agcaaaaact cgcaaaaaag ccaaaaacaa aaattctccc cgcaccaatc
    97201 ccacaacgcc tgtcgtagag ttcaatcccc agctcaaaac cgtgaaaatc tttagtgatg
    97261 gctcttgcct taaaaatccg ggtggcccgg gtggttacgg tatcgttctc cagtatcgtg
    97321 gtgaggaacg cgagttctca gatggttttc atagcaccac caataaccgc atggagatga
    97381 tgggcgcact tatcgggctg gagcgtttga aatatccatg caacgttatt ttgtactctg
    97441 atagccagta tctaaaaaac ggcatgacac agtggatgaa atggtggaaa cgtaatggat
    97501 ggatgacttc tgataaaaaa ccggtaaaga atgttgatct gtggaaacgt ctggatgagg
    97561 ccgcaagtcg acataatgtt cgctggaagt gggttaaagg tcacgccggg catcgtgaaa
    97621 atgaaatatg tgatcgactc gcgaagatcg cagctttttc cgcagccgat acgcctcaca
    97681 agaaagacat tggttttatt tacaacaagt ggtaagtaag tgtttaccta tcatattaaa
    97741 tcatgtatct tatcggcgtc aggatgacaa tgtgtcggta agacacagtt ccaggatgga
    97801 acgagaaagg cggctggcaa tcgccagccg caactctttc tgacactgga tggagtccac
    97861 atggcacgtc aaacctattt cacttctgca actaaacgtc ctcgttcact acgtcaaatt
    97921 ttggccgaat tatttagcgg tcgtgttctg tcacgtcttg atgaactgga gactaccgtt
    97981 cggttgctga atgaacgttt agataagcaa gcgtcagttg ttgcgaacgt gggggcgatt
    98041 gttacctctg gttcttcaca ccaagcgaaa aatgcacggc ctttagtgaa ggagaaaaac
    98101 aacaaggaca gttcgaatgg aaaattttca aagaaagagg ctgaaaccaa tggcctacgt
    98161 tctcattata gtttcactgg cgacggtagc cgttccagcc ggccagagcc ttttgatgcc
    98221 gggttcatcc atcaccacac ctccgtcgac gacaattacc accactccag tagagcgtcc
    98281 tgtcactctg gatgggatga cggtggatgc gatacctcaa gttcatccag ttactcagga
    98341 tcatgctgtg actaaggcgg ttgtatgaac tggttttcaa atcactttgg aaaaatttgg
    98401 ctggcaattc ttgccctcat ggccgccggt tgggtatcga acattataaa acttgtttgc
    98461 tctggcgatc tccagtttca ggctggtatg accttggctc gtgttgttgg gatttttgtt
    98521 tttccagttg gttcggtact tggttatttc tgacggctgt tagtgcatat gcattgacca
    98581 tctctacgta agcaatttat gtaattagaa aacaacttgt tttgacaaat aataaaagga
    98641 aaacacatgt taggtttctt caaaaagaaa actcgtaaag ctgttatcga agtcaaaaaa
    98701 atggagaacc gtgatgcggt tgaggccacc gtgtggggcg cgtacatgat ctcctatgcc
    98761 gacggcacat gtgacgcaaa agaaattgcc attcttgaga agacaattgc agctctgcct
    98821 gcgttttctc cgtttgctgg tgaaattgcc caaatgagcg ccaatatccg tgctcgttac
    98881 gaagcgtccc ctcgttctgc taatgcacag gctattcgtg agctgtctga tattgcgggt
    98941 actccagagg cggtcgatgt tctgtgtctg tgccttgata ttgccgatca ggacggcatt
    99001 ggtgaacaag aagagcaggc acttaaaaag atcgcccagg cgcttcagtt gtcactggat
    99061 gcttatctct aatgcttgag agattccggc ttgtgatcgt cattgctctt ctggtgatgg
    99121 cggtgttggt ggattttacg ggaaagatga tgtctgtcat ttctgatggc gtcctcattg
    99181 gtctggcgat ctacttcgct tatccgctag tccgtaaagc aacgtgttaa tgacaagggc
    99241 caaatggccc ttgtgtttcg ttgaccgaaa tagagagttt gcacctttac gtttagcttg
    99301 ctcccctttt atgccacatc acaataaagc caataagaaa acaacttgtt taagcattag
    99361 gaaaaacaca tgtgcaagaa atgcaaagcg atagctgatg aacaaaacgc cttattcgaa
    99421 gaaatggatg ctaatgaact ggtcaaaatg ttagccattc ttcgaggaat agaagacgtt
    99481 tccatatttg agagagtggt tacagcactt aattttgagt ccacctttga agagccaact
    99541 caggttgtag ctttagcaca tcatttcggt gttcattatc ttgctgaaaa agagcgcgct
    99601 gataagttgc aggcgacttt ggatatggtg agcgagactc aacgaactga tgacactaac
    99661 aagagtgagg cgattattgc cagcaaagat cgtgaaattg ctgggcttaa atcctctcta
    99721 acgatgttga tgtctgcgtt caatcttatg tcttctcagg cgggttataa aatgccatca
    99781 ctaaacagcg atgatccgat ggccgttcgt cagcttttgg gagcaatggc cgaccaactc
    99841 gacgacacaa agagtcggct tgaagacatg atgcgtgagt taagccatcg acataacctc
    99901 gcaacacagc cacacaaagc ctttcaaagc tctaattgat ctgatatggc cgcatgatcg
    99961 gccatattgt gttgataaaa aacaaggatg aaaaatggca tacggtacag ggatttacaa
   100021 caataaagga attaacgtta ctggcttcct tactcctatt ttttttcttg atcgatttac
   100081 tgcgtcatca ggctctaaga cgtactccaa tccgccaccg ggtaaatcgc tacatgccgt
   100141 gtggtcattg atgcctctta acaacgacaa ctacattaat ttacctgttc caacagttac
   100201 tattaatgga aatacggtta gttggtcaaa tttatatacg gggcttggtt cttacatata
   100261 cacatactgg ggataattat gttcgggatg tcaattactc aatcagatgg aagtttgtgg
   100321 atgagtccag aatttactcc gcaaaatctg attaataaag ggacaatgtc tacatctaaa
   100381 ggttctgttt ttcaaacatc aatcccatca aacaaatcat gctttttctt cataaaaagc
   100441 agtaataagg cgaacatgat gtttattcat gagcatagta acggatacaa tgctctcaga
   100501 ttgcatcagg taaatggtag ccccggaaca ataacagttt atgctttttc tgatatggtg
   100561 ttaccacatt ctggctatgg cattgccatg tataacagcg caggcgcaat ggtgtatcac
   100621 ggtgagatga tgccccttga tgcgaaactt atcactattt ctgaccctca atttacaata
   100681 gatatggggt atccatgtgc ggtaatgcct gctatggttg gggtatataa ctatagacga
   100741 accgattacg acagacctgt ctatgtaaca atgacaggtg caactggaaa ccaagtatat
   100801 aacggtcaat ggtattccgg taatgtcaca tgggatatta agaagattta tacaaacaaa
   100861 attctggtga taaatacctc caagtatgat tagccaatgc ctttatttga aggattgcct
   100921 gtttatttct atttagtacc ttttcatttt tgaaataatt gcaccattaa acaaatcact
   100981 tcttaatggt gcaattatga atacagccct ttccatcatc gacgatgcca actcaaacac
   101041 tgctatcgac tatcggcagg aaatgaacgt catccacgaa attgtggccg agtgcgagaa
   101101 agagatcgcc ttcatgtatc aggtacacga cttcgtttat ggcgacgaac gccacaacat
   101161 gattaatcgc ctgctgagac taaaccatcg accagatgaa gatcgctcgc gtttaaatcg
   101221 aggttggttg gataaagtcg atctggaatg ggtgaaacag aatatttggg ccgagtactg
   101281 gaggaaggtc acggacatga ctaacgtttt gctgatcatg ccagcttccc gtcgagatga
   101341 gtggcgcgag cagtttatag agggcaaaca ggaagtcatc aaaactgaca gaaccggcta
   101401 ccagatgaag gttaaagagt tcgttggtgt accggagttc aaagcagaaa cggtcatacc
   101461 cacgatgctt aatttgctga atgacaggca caaatatctc tctgagcgcg tgtatggctt
   101521 gtttaaggcg ctgagtcctg cgcacaagac aaataagaca aacggtttca gcgaacgtct
   101581 gataatcgct gactgcattt ctgatttctg gcgggacagc gttagtgtga actatcgcaa
   101641 agaggactat atcgacgatc tgcgtgtctt gcttcatttc ttcgcgcaca aagaatttat
   101701 taccatcaac cgcactgctg aggtgctatc agctgcgtat cgggcaaacg actgccagac
   101761 cggtgactgg atgaacgtcg atggaaatct aatgcgcgtg aagatgttca agaacggcaa
   101821 cgttcacttt gaaatacatc ctgacgtggc ctggaagttg aatgaggtgc tggcttacag
   101881 tatgcctgct gcaatccccg cgccatgtcg caccgcgcca aaaacacggg caccaaagca
   101941 gttcgggtta atccagaaga cgatctccgt gccggttcgc actgcgcttc gtgacgggcg
   102001 attaagcaaa gacaaaggcg tatggtactt ctctgattca gctctccaga agtcgcaggt
   102061 ggaagagctt gagcgcacac tgagcttcat tggcggcgtg caggagaaaa agcactggca
   102121 gttcccgtat gacatcggcc atacgctaaa tacgattgtg gctaccggtt taataccgga
   102181 tacaaaatca caccagttct accctacccc acgcttgatt gctgagtacg ttgccagagc
   102241 cactgaattg aagcctggtg agaagctgtt ggagcctcaa gccggacgtg gggatcttct
   102301 ggcctatatt aatgccgatc tggaagatgt tacttgcata gaaatcgcac ctcttttcgc
   102361 tgatatcctg cgtggaaaag ggtatacgaa cacgatttgc tgcgacttca taaagtggtc
   102421 tgaggacaac gcaggttatc agttcgacaa aatcgttatg aacccgccgt actcgcttgg
   102481 tcgtcataga gaacacacgc ttgctgcgct ggggcatctg aaagtcggcg ggcgtcttgt
   102541 agcagtattg ccgggcactg cgccaatact ggactggatg acgatggata attacgttta
   102601 tgccagaggg aagtcgttta ccaacgagtt tgaagacaca gggatcacag tcagcgtata
   102661 cgttttcaaa cgcgttaaat gataggtaaa tacttaccta atttgtgtaa gaatgtagtt
   102721 actaaacgat aagagaaaaa cacatgaaca acctccagtt agagcatttt aacgtcacag
   102781 gccattctga ttttcctttc aagtttacat tgaaaggtta tgcagaggat gcggtaggcc
   102841 agatcattat tgataaaggc atcgttaagt ttgaggggaa ttttgatgaa tccgcgaaaa
   102901 cattcataga cttcgttgcc aaacgttgga gcgagcaatg gaaagacctg gaaaagcgcg
   102961 ctagtgagtt tgatcggttc atggatgcaa tggatacagc aaaagaggct cttgatgccg
   103021 ggactccgtt agatctggag tcacttttca acggcgaagt ggcctctgcg atgttcgcca
   103081 ccatgttcgc gggtgagttc gtccgcagcg gtgccaaaaa ctaccttgag ctggattaca
   103141 acgtccctgc aattggcgat ttcgtcgtta ccatccaacg caaagaaggt aagacgccag
   103201 gtgaacgcgt cgcagagctt gagaccgttg tggatcagcg taacggagag tgtgaccgtt
   103261 tgatcaacga gcttcatgca cttcgggaag aaagaatatg cgcgggtagc aatacacgta
   103321 atgcagcgga tatctacttc cagttagttg aggaatgcca gattccacca ggcggctctc
   103381 ttgtcgatta cgtcagacat ttaatgacgg aagttaactc cagccataaa gatggtgagg
   103441 tgcgctgatg tttggcattg acgcacagcg tgtagccgct tttgcaaaaa gccctcttga
   103501 taatcccttg tctcgtagtg agcaaatgga gctggcaagg ctttttcttc acattcaaaa
   103561 acaggcagac attttcaata acatgcctaa tcaacctatt ctggatggtc acatccagat
   103621 ggtcattaac agtcatgaga aaggctgggc tgcaatcgtc ccctgcacaa ttacatacag
   103681 gttggcgaaa gaggttcaag agtttcgaaa agccagtgtc gaatcggagt ctaccaaagc
   103741 cgcaataaac actcttattc gcatggggtt cacatggacc ggtggagcct actggcaagc
   103801 acctcacccc attttatccg gcaattagac gctacaaatc cttcggcatg aataaataaa
   103861 ggccataagt tgtggcctta aataaattgt tttctacctt ttcttatttg tgaaaataac
   103921 cagcacttgg tagtgcttat gtaacagcaa agaagagaaa aacacatgag taacaaaatc
   103981 gaaaatcccg tagttctcat tcacaagcgc gagaaccacg actcctatgc ggtggcgatc
   104041 accaatggga gtcacgattt ttacgatggc ctgctaatgg cctcagtgtc gcctgatgag
   104101 gcagacaact cttttgccgt cttcgctatg gttggttact acatggctgc cgaaattgag
   104161 aagttgcggg cgcagagaga cgcattagcg gcggagaatg tggcattgcg atctaaggct
   104221 gcggaactgg cacatgaagc gtcaaaaatt tactcggcat ataacgccac tatcacagag
   104281 cctgacgggg attttatgga catgcagaca ttgcatgaaa tgcagtgcat agaaactcca
   104341 gccaccgatg cttttctggc tgaagtacgt gcgcagggcg tggatatggc tcgtaacgcg
   104401 atgattgatt ttgttgatgg tgaagttggg ccaaacaaga acgttccggg gctgattaga
   104461 ggcgcagaga tatgcgtaag tattgctgaa cagcttcgca aaggaggcaa ccagtgagca
   104521 agattgacta tcaggcactg cgtgaaaagg cagagaaagc aacgtgtggc gagtgggcgc
   104581 tagaatatgg agatggcaga tttgatggtg atgatgcact aattcatcgc gaggctgctg
   104641 gatatattcc catttgcaga attgaaggag cgcatcctga aagcggtttc gatgaagatt
   104701 tccaaatgga gcagcaggcc aatgctgaat tcatcgccgc agccaacccg gctactgtgc
   104761 tggtattact ggatgaacgg gaaaggaacc agcaatacat caaacgccgt gaccaggaga
   104821 acgaggatat tgcgctaacg gttgggaagc tgcgcgttga gctggaagcc gcagagaagc
   104881 gcattgctga actggagtca ctgatggaac caaagttgcc gcagccagcg gtggttgaca
   104941 acgataagtt ccgagcggat tttgaacggt ggatggttga ggatgaaaag tgcatcgtag
   105001 gaagctctga cccttatcca gctgggattg agtcacgcaa ctggagagca tggaacgcct
   105061 gccgcgccgc catgcttcag agtcaagata gtggcattaa ggatagctgg ataagctgta
   105121 gtgagcgaat gccggatacc aaaacagccg ttcttgttgc caaggagttt gataggaaag
   105181 gtgactggcg aatgaaatgg gcgacttaca tcccggggca tcctgacgct aatgatgggt
   105241 ggatcattcc tggggcgtcg tggaaaccgt cacactggat gcagctacca gaacctccgc
   105301 aggagtcgca gagtgaataa agcagagtta tttcagaaaa tatcggctct cgcgactgaa
   105361 tgtcacgcta tagcatctga gcttgatgtt ggcggtgaac gaaccgagat gttcgaaata
   105421 tacagtgtgc tgcgcaatct ctgtcggcgt ggctacgcca ctcaagtagg gcgaatgact
   105481 aacccactac tctcatcctg tgatgaggat gactcggatg aggatgacga atgatgcata
   105541 aatcagtagc cggtgagttt cagaaggaag tcgataatac taccgttcta ttggacgata
   105601 ttttaagcat tctcgcgctg cttgaggctg gcgattggtc agaacattgc actaaaacag
   105661 agctaggcgg tcggcttgaa agagagatta cacgacttat tggtgatgcg caggaagcta
   105721 cagtcactag ttatgagtta atcgctgaag cctggcgttt gatggatgga caagatccta
   105781 aaaccagcga ttggcatagc aaggcttcga agtatttaaa ttccaatagg gtagaaaaag
   105841 ttgatgatgt gaaaccgaag ccagtagacc acggtttccg cgataactgc gaatgctcta
   105901 gttgccagac cacggcccgt atttgttccg aattgacaga taagtccagc ctaatctacg
   105961 aagttaatgt aggcggtaat acatgggtcg aatgcacaag agctgcatac gtaagagcaa
   106021 aagacaaggg tgaattaacc agagttgttg cccatcaccc aaataatgag cttaaagatc
   106081 accagattcg agaactggtg aacgagttga gagatattgc ggttcagtac cacggaacgc
   106141 agcaattgcg ggagaagatt gccagagcag tgaacaactc gctcaaaggt ggacagtccc
   106201 aaccgaaacg tatacgaatt acacatatag gtgaaaacgg aacagaaatc ccccaatttt
   106261 ctgttgggga catctttgat gtacatggag aagagtttgg tgagcctcac atcactatgc
   106321 ctagtggcca tgtttactgg ctaaaagcta tggagcaata tggtgataaa tgggaagtgt
   106381 tatgagggat gcgaagttat gatcaaattt ggaaagctgg aagctcattt gctgactcgt
   106441 aactaccgcc atgatataca cccatataga cagtggattg atgaaagcga aggagttgtt
   106501 acattccctc tatacaggtt tgatgggatg ttggccgggt atcagacata cagaccaggc
   106561 gctcctaagc agcatagcaa tccgaagatg gcccggtatt tcacacgagc gaacggcaga
   106621 cagctactat ggggaaccta tcttccttta aaagatggac cgatatggat aactgagtcg
   106681 atttttaaaa gtgcagcagt gcacaacgcg ggcggaaatt cgtgggcgct gttaggttcg
   106741 actttctcag caggattacg tcgtcaactg gcaatgctgc cgtatgactt tcgcgtaata
   106801 ggtgacaatg atgcggctgg tgaatcactt gtgaagtctt tcggaaaagg ttttgtggcc
   106861 ccggatcttg acgagctaca accaaatgaa gtgtctcatt tgatttttag ccatagccaa
   106921 taagcatccc ttctccatta aggccactag ttgtggcctt aaacaatttg ttttctacct
   106981 tttcttgttc gagaaaataa cgttcctttg tctggtgcta tgtaccgatc agttgcactg
   107041 agtgagcttc tttcatgtat actgtataaa tgaacagtat tgagggcaaa acgctatggg
   107101 cttcccttct cctgcggcgg attatgctga gagccgtatt tctcttgatc agcagataat
   107161 tagacatcct tcagcgacct acttcatgag agcggctgac agccatcatc gtgagggaat
   107221 attgcagggc gcattgctgg tggtcgattc ctcacttacc ccggttgatg gttctctgct
   107281 tgtatgcgct ctggatgggg aatatcgcgt aaaaagatac cgtaagtatc cacgtcagca
   107341 tctggaggat ttaagaactg gtaagaagga agcattgcca aaggatgacg atggatgcac
   107401 gggcagcaat gccgtgtttg gtgtgatcac tcacattatc aacgacgcaa gaagtggcga
   107461 gtttgatgat tgtcctgtga tgtaggagaa ctgtttaggt ggtgcaatgc accacctttt
   107521 tatcacactg cgcggaatgc gatttcgcca ggtattactt caccttgcca atacatttgg
   107581 gcagcaacgc gatctgcgag gtcacgataa atagctgtaa attcgctatc tggacgacta
   107641 ataacggttg gtgttccgtt atccagatct tcacgaagag agatatgaag tggcatttgg
   107701 cctaacaact gcgtgttgta tttctcggcc agtttctgtg cgccaccggt gccaaaaatt
   107761 ggctcgtgat gaccgcagtt actgcaaata tgcacactca tgttttcgac gatacccagt
   107821 accggcactt cgactttttc gaacatcaca atgcctttct tcgcatcgat cagcgcgatg
   107881 tcttgcggcg tagttaccac aaccgcacca gttacaggaa tgttctgcgc cagcgtcaac
   107941 tgaatatcac cagtgcccgg cggcatatcg agaacgagat agtccagatc aggccataga
   108001 gtttcctgca acatctgcat cagcgccttg ctggccatcg gtccacgcca caccattgca
   108061 ttgtcgtcgg tgaccagata accaatagag ttggttgcca ggccatgaga catgataggt
   108121 gccatgtgag taccgtcagg tgaggttgga cgttggtttt ccgcgcccag catggttgga
   108181 attgatggac catagatatc ggcatccaaa ataccaactt tcgcaccttc agcagccaac
   108241 gccagtgcca ggtttaccgc cgtggaggat ttacccacgc cgcccttgcc tgagctgacg
   108301 gcgataatgt tcttaacgcc attaatgcct ggttggtttt tgacgcgctt aagcgtggca
   108361 atgttgtacg acagcttcca gtcaatagcc tttgcgccag tgatacggag cagatcacca
   108421 ctacattgct ctttcaggtc ttcaaaaggc ttattccaca cgaaaggcat gattagttcg
   108481 acatgcagtg tgtcatccat caacgcaaca tggtgtaacg ctttaagcgt agtcaggttg
   108541 tgtttcaggg ttgggtgctg aaaattagcc agcgtaccgg ctaccattgc tctcagggca
   108601 tccggcgatt tggactcgct catcccgtct cctttatttt aatttgcgca attgtcgcct
   108661 tgtagtgtac tccagctacg acatttaatc atttatgaga aatgctgtta tcacatggca
   108721 gacataaggc cattttgtta ctatcaagcc ccttttcact acaaagaagt aatgcctact
   108781 atgacccaag tcgcgaagaa aattctggtg acgtgcgcgc tgccgtacgc taacggctca
   108841 atccacctcg gccatatgct ggagcacatc caggctgatg tctgggttcg ttaccagcga
   108901 atgcgcggcc acgaggttaa tttcatctgt gccgacgatg cccacggtac gccgatcatg
   108961 ctgaaagcac agcagcttgg tatcacaccg gagcagatga ttggcgaaat gagtcaggaa
   109021 catcagactg atttcgcagg ctttaacatc agctatgaca actatcactc gacgcacagc
   109081 gaagagaacc gccagctgtc tgagcttatc tatactcgcc tgaaagagaa cggttttatt
   109141 aaaaaccgca ctatctctca gttgtacgat ccggaaaaag gcatgttcct gccggaccgt
   109201 tttgtaaaag gcacctgccc gaaatgtaaa gcgccagatc aatacggcga taactgtgaa
   109261 gtctgcggcg caacatatag cccgactgaa ttgatcgagc cgaaatcggt ggtttctggc
   109321 gctaccccgg taatgcgtga ttctgaacac ttcttctttg atctgccctc tttcagcgaa
   109381 atgttgcagg catggacccg cagcggcgcg ttgcaggagc aggtggcgaa caaaatgcag
   109441 gaatggtttg aatccggcct gcaacagtgg gatatctccc gcgatgcgcc ttacttcggt
   109501 tttgaaattc cgaacgcgcc gggcaaatat ttctacgtct ggctggacgc gccgattggc
   109561 tacatgggtt ccttcaagaa tctgtgcgac aagcgcggcg ataccaccag cttcgatgaa
   109621 tactggaaga aagactccac cgccgagctg taccacttca tcggtaaaga tattgtttac
   109681 ttccacagcc tgttctggcc tgccatgctg gaaggcagca acttccgcaa gccgaccaac
   109741 ctgtttgttc acggctatgt gacggtgaac ggcgc
//
LOCUS       DAJTVK010000002        88079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000002 DAJTVK010000000
VERSION     DAJTVK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 88079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 88079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..88079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.002"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO372_000126"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="OO372_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6339748.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="OO372_000127"
     tRNA            1230..1305
                     /locus_tag="OO372_000127"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO372_000128"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="OO372_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6339749.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO372_000129"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="OO372_000129"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6339750.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO372_000130"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="OO372_000130"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6339751.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO372_000131"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="OO372_000131"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6339752.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="OO372_000132"
     CDS             complement(4868..5305)
                     /locus_tag="OO372_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR6339753.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="OO372_000133"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="OO372_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO372_000134"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="OO372_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HCR6339754.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            7987..8271
                     /gene="pduA"
                     /locus_tag="OO372_000135"
     CDS             7987..8271
                     /gene="pduA"
                     /locus_tag="OO372_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HCR6339755.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            8268..9080
                     /gene="pduB"
                     /locus_tag="OO372_000136"
     CDS             8268..9080
                     /gene="pduB"
                     /locus_tag="OO372_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HCR6339756.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            9099..10763
                     /gene="pduC"
                     /locus_tag="OO372_000137"
     CDS             9099..10763
                     /gene="pduC"
                     /locus_tag="OO372_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HCR6339757.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            10773..11441
                     /locus_tag="OO372_000138"
     CDS             10773..11441
                     /locus_tag="OO372_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HCR6339758.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            11456..11974
                     /gene="pduE"
                     /locus_tag="OO372_000139"
     CDS             11456..11974
                     /gene="pduE"
                     /locus_tag="OO372_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HCR6339759.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            11984..13816
                     /locus_tag="OO372_000140"
     CDS             11984..13816
                     /locus_tag="OO372_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HCR6339760.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            13806..14156
                     /locus_tag="OO372_000141"
     CDS             13806..14156
                     /locus_tag="OO372_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HCR6339761.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            14176..14451
                     /gene="pduJ"
                     /locus_tag="OO372_000142"
     CDS             14176..14451
                     /gene="pduJ"
                     /locus_tag="OO372_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HCR6339762.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            14475..14897
                     /locus_tag="OO372_000143"
     CDS             14475..14897
                     /locus_tag="OO372_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HCR6339763.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            14900..15532
                     /locus_tag="OO372_000144"
     CDS             14900..15532
                     /locus_tag="OO372_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HCR6339764.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            15529..16020
                     /gene="pduM"
                     /locus_tag="OO372_000145"
     CDS             15529..16020
                     /gene="pduM"
                     /locus_tag="OO372_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HCR6339765.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            16024..16299
                     /gene="pduN"
                     /locus_tag="OO372_000146"
     CDS             16024..16299
                     /gene="pduN"
                     /locus_tag="OO372_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HCR6339766.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            16311..17318
                     /gene="pduO"
                     /locus_tag="OO372_000147"
     CDS             16311..17318
                     /gene="pduO"
                     /locus_tag="OO372_000147"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HCR6339767.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            17315..18700
                     /gene="pduP"
                     /locus_tag="OO372_000148"
                     /pseudo
     CDS             17315..18700
                     /gene="pduP"
                     /locus_tag="OO372_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            18711..19823
                     /locus_tag="OO372_000149"
     CDS             18711..19823
                     /locus_tag="OO372_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR6339768.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            19820..21165
                     /locus_tag="OO372_000150"
                     /pseudo
     CDS             19820..21165
                     /locus_tag="OO372_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            21168..21722
                     /gene="pduT"
                     /locus_tag="OO372_000151"
     CDS             21168..21722
                     /gene="pduT"
                     /locus_tag="OO372_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HCR6339769.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            21722..22072
                     /gene="pduU"
                     /locus_tag="OO372_000152"
     CDS             21722..22072
                     /gene="pduU"
                     /locus_tag="OO372_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HCR6339770.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            22077..22520
                     /locus_tag="OO372_000153"
     CDS             22077..22520
                     /locus_tag="OO372_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HCR6339771.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(22621..22950)
                     /locus_tag="OO372_000154"
     CDS             complement(22621..22950)
                     /locus_tag="OO372_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR6339772.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(23122..24180)
                     /locus_tag="OO372_000155"
     CDS             complement(23122..24180)
                     /locus_tag="OO372_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6339773.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO372_000156"
     CDS             complement(24378..24851)
                     /gene="sbmC"
                     /locus_tag="OO372_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCR6339774.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO372_000157"
     CDS             complement(24970..26136)
                     /gene="dacD"
                     /locus_tag="OO372_000157"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCR6339775.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            26345..27772
                     /gene="sbcB"
                     /locus_tag="OO372_000158"
     CDS             26345..27772
                     /gene="sbcB"
                     /locus_tag="OO372_000158"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6339776.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(27815..28042)
                     /locus_tag="OO372_000159"
     CDS             complement(27815..28042)
                     /locus_tag="OO372_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6339777.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO372_000160"
     CDS             complement(28056..29114)
                     /gene="yeeE"
                     /locus_tag="OO372_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6339778.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO372_000161"
     CDS             complement(29293..30651)
                     /gene="plaP"
                     /locus_tag="OO372_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6339779.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO372_000162"
     CDS             complement(30641..30703)
                     /gene="yoeI"
                     /locus_tag="OO372_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6339780.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(30918..31847)
                     /locus_tag="OO372_000163"
     CDS             complement(30918..31847)
                     /locus_tag="OO372_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6339781.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(31893..32717)
                     /locus_tag="OO372_000164"
     CDS             complement(31893..32717)
                     /locus_tag="OO372_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6339782.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO372_000165"
     CDS             complement(32800..33054)
                     /gene="yoeB"
                     /locus_tag="OO372_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6339783.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO372_000166"
     CDS             complement(33051..33302)
                     /gene="yefM"
                     /locus_tag="OO372_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6339784.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            33585..33635
                     /gene="hisL"
                     /locus_tag="OO372_000167"
     CDS             33585..33635
                     /gene="hisL"
                     /locus_tag="OO372_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR6339785.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    33611..33734
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            33781..34680
                     /gene="hisG"
                     /locus_tag="OO372_000168"
     CDS             33781..34680
                     /gene="hisG"
                     /locus_tag="OO372_000168"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6339786.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            34686..35990
                     /gene="hisD"
                     /locus_tag="OO372_000169"
     CDS             34686..35990
                     /gene="hisD"
                     /locus_tag="OO372_000169"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6339787.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            35987..37057
                     /gene="hisC"
                     /locus_tag="OO372_000170"
     CDS             35987..37057
                     /gene="hisC"
                     /locus_tag="OO372_000170"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6339788.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            37057..38124
                     /gene="hisB"
                     /locus_tag="OO372_000171"
     CDS             37057..38124
                     /gene="hisB"
                     /locus_tag="OO372_000171"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6339789.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            38124..38714
                     /gene="hisH"
                     /locus_tag="OO372_000172"
     CDS             38124..38714
                     /gene="hisH"
                     /locus_tag="OO372_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6339790.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            38714..39451
                     /gene="hisA"
                     /locus_tag="OO372_000173"
     CDS             38714..39451
                     /gene="hisA"
                     /locus_tag="OO372_000173"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6339791.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            39433..40209
                     /gene="hisF"
                     /locus_tag="OO372_000174"
     CDS             39433..40209
                     /gene="hisF"
                     /locus_tag="OO372_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6339792.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            40203..40814
                     /locus_tag="OO372_000175"
     CDS             40203..40814
                     /locus_tag="OO372_000175"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6339793.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO372_000176"
     CDS             complement(40911..41888)
                     /gene="wzzB"
                     /locus_tag="OO372_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6339794.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO372_000177"
     CDS             complement(42034..43200)
                     /gene="ugd"
                     /locus_tag="OO372_000177"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR6339795.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO372_000178"
     CDS             complement(43450..44856)
                     /gene="gndA"
                     /locus_tag="OO372_000178"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6339796.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO372_000179"
                     /gene_synonym="manB"
     CDS             complement(45020..46390)
                     /gene="cpsG"
                     /locus_tag="OO372_000179"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6339797.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO372_000180"
     CDS             complement(46495..47931)
                     /gene="cpsB"
                     /locus_tag="OO372_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6339798.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO372_000181"
     CDS             complement(47934..49157)
                     /gene="wcaI"
                     /locus_tag="OO372_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6339799.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO372_000182"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(49154..49633)
                     /gene="gmm"
                     /locus_tag="OO372_000182"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6339800.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO372_000183"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(49636..50601)
                     /gene="fcl"
                     /locus_tag="OO372_000183"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6339801.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO372_000184"
     CDS             complement(50604..51725)
                     /gene="gmd"
                     /locus_tag="OO372_000184"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6339802.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO372_000185"
     CDS             complement(51751..52299)
                     /gene="wcaF"
                     /locus_tag="OO372_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6339803.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO372_000186"
     CDS             complement(52315..53061)
                     /gene="wcaE"
                     /locus_tag="OO372_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6339804.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO372_000187"
     CDS             complement(53072..54289)
                     /gene="wcaD"
                     /locus_tag="OO372_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6339805.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO372_000188"
     CDS             complement(54264..55481)
                     /gene="wcaC"
                     /locus_tag="OO372_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6339806.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO372_000189"
     CDS             complement(55478..55966)
                     /gene="wcaB"
                     /locus_tag="OO372_000189"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6339807.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO372_000190"
     CDS             complement(55969..56808)
                     /gene="wcaA"
                     /locus_tag="OO372_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR6339808.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO372_000191"
     CDS             complement(56901..59063)
                     /gene="wzc"
                     /locus_tag="OO372_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6339809.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO372_000192"
     CDS             complement(59066..59509)
                     /gene="wzb"
                     /locus_tag="OO372_000192"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6339810.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO372_000193"
     CDS             complement(59515..60654)
                     /gene="wza"
                     /locus_tag="OO372_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6339811.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            60963..61112
                     /locus_tag="OO372_000194"
     CDS             60963..61112
                     /locus_tag="OO372_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339812.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            61313..62896
                     /locus_tag="OO372_000195"
     CDS             61313..62896
                     /locus_tag="OO372_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6339813.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO372_000196"
     CDS             complement(63158..65023)
                     /gene="asmA"
                     /locus_tag="OO372_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6339814.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKMLVTR"
     gene            complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO372_000197"
     CDS             complement(65045..65626)
                     /gene="dcd"
                     /locus_tag="OO372_000197"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6339815.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO372_000198"
     CDS             complement(65718..66359)
                     /gene="udk"
                     /locus_tag="OO372_000198"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6339816.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            66677..69994
                     /locus_tag="OO372_000199"
     CDS             66677..69994
                     /locus_tag="OO372_000199"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6339817.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO372_000200"
     CDS             complement(70104..70952)
                     /gene="alkA"
                     /locus_tag="OO372_000200"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR6339818.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            71086..72438
                     /gene="yegD"
                     /locus_tag="OO372_000201"
     CDS             71086..72438
                     /gene="yegD"
                     /locus_tag="OO372_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6339819.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO372_000202"
     CDS             complement(72451..74391)
                     /gene="yegI"
                     /locus_tag="OO372_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR6339820.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO372_000203"
     CDS             complement(74388..75149)
                     /gene="pphC"
                     /locus_tag="OO372_000203"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR6339821.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(75146..75805)
                     /locus_tag="OO372_000204"
     CDS             complement(75146..75805)
                     /locus_tag="OO372_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6339822.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO372_000205"
     CDS             complement(76026..76085)
                     /gene="ibsA"
                     /locus_tag="OO372_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR6339823.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(76358..76414)
                     /locus_tag="OO372_000206"
     CDS             complement(76358..76414)
                     /locus_tag="OO372_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6339824.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(76685..76741)
                     /locus_tag="OO372_000207"
     CDS             complement(76685..76741)
                     /locus_tag="OO372_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6339825.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            77020..78261
                     /gene="mdtA"
                     /locus_tag="OO372_000208"
     CDS             77020..78261
                     /gene="mdtA"
                     /locus_tag="OO372_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR6339826.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            78261..81383
                     /gene="mdtB"
                     /locus_tag="OO372_000209"
     CDS             78261..81383
                     /gene="mdtB"
                     /locus_tag="OO372_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR6339827.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            81384..84461
                     /gene="mdtC"
                     /locus_tag="OO372_000210"
     CDS             81384..84461
                     /gene="mdtC"
                     /locus_tag="OO372_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6339828.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            84462..85877
                     /locus_tag="OO372_000211"
     CDS             84462..85877
                     /locus_tag="OO372_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6339829.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            85874..87277
                     /gene="baeS"
                     /locus_tag="OO372_000212"
     CDS             85874..87277
                     /gene="baeS"
                     /locus_tag="OO372_000212"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6339830.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            87274..87996
                     /gene="baeR"
                     /locus_tag="OO372_000213"
     CDS             87274..87996
                     /gene="baeR"
                     /locus_tag="OO372_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6339831.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        21561 a        23316 c        22148 g        21054 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact accctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaacaacc aataaattaa caacatatag
     7861 ttcataaaag caattttata atatttgttc tctattaatt tctctcatta gccgattaag
     7921 tcagccattc tttttcctcg ctttttttta tagtcatatc gtcaaaaaaa tccaggcgag
     7981 gtctttatgc aacaagaagc gttaggaatg gtagaaacca aaggcttaac tgccgccata
     8041 gaggccgcag acgccatggt gaagtcggca aatgttttgc tggtcggcta cgaaaaaata
     8101 ggttccgggc tggtgaccgt catcgttcgt ggtgatgtcg gggcggtaaa agcggcaaca
     8161 gacgcgggtg ccgcagctgc gcataacgtc ggcgaagtaa aagccgtaca cgttattccc
     8221 cgcccacata ccgatgtcga aaaaatctta ccaaagggaa ttaacccatg agcagcaatg
     8281 aactggtcga acagatcatg gcgcaggtca tcgctcgcgt agcgacgcca gagcatgagg
     8341 ttctcccgaa aaccactcat caaatacgag agacggctat ggcagaaaaa agctgcacgc
     8401 taacagaatt tgttggcacc gctattggcg acactgtagg tctggtcatt gcgaacgttg
     8461 atagcgccct gttggacgcg atgaaacttg aaaagcgcta tcgttccatt ggcattctgg
     8521 gtgcgcgtat tggcgcaggg ccacacatta tggccgctga tgaagcggta aaagccacca
     8581 acactgaagt tattagcatt gaacttcctc gcgatactaa aggcggtgcc ggacacggct
     8641 cactgatcct cttaggtggc aacgatgttt ccgacgtgaa acgcggtatt gaagtcgccc
     8701 tgaaagaact cgatcggacc tttggcgatg tctatgccaa cgaagccgga catcttgaac
     8761 tgcaatatac cgcccgcgcc agctatgcac tggaaaaagc gtttggtgca ccactgggac
     8821 gggcttgcgg cgttattgtc ggcgctccgg ctgctgtggg tgttctgatg gccgacaccg
     8881 cgctgaagtc cgccaatgtg gatgttgtgg catacagctc cccagctcaa gacaccagct
     8941 acagcaatga agtcattctg attatctcgg gtgactccgg cgcagtccgc caggcggtca
     9001 tctccgcacg cgaaatcggt aaaaccgtgc tttcgactct cggcgataca ccgaaaaacg
     9061 atcacccttc ctacatctga attctctgag gcagatttat gagatcgaaa agatttgaag
     9121 cactggcaaa acgcccggtc aaccaggacg gtttcgttaa agagtggatt gaagaaggct
     9181 ttattgcgat ggaaagcccg aacgatccta aaccgtcaat caaaattgtt aacggcgtcg
     9241 ttaccgaact ggatggaaaa cccgccagcc agtttgacct gatcgaccac tttatcgccc
     9301 gctacggcat caatctcgcg cgcgccgaag aagtcatcgc gatggattcg gtaaaacttg
     9361 ccaatatgtt gtgcgatcca aatgtcaaac gtagcgatat cgttcccctt accacagcaa
     9421 tgacgcctgc aaaaatcgtc gaagtggttt cgcagatgaa cgtggtagag atgatgatgg
     9481 cgatgcagaa aatgcgcgcc cgtcgaacac catcacaaca ggctcacgtc accaacgtca
     9541 aagataaccc agtacaaatt gccgccgacg cagctgaagg cgcatggcgc gggtttgatg
     9601 aacaggaaac caccgttgcc gttgctcgct atgcaccgtt caacgccatc gccctgctgg
     9661 tcggctcaca ggtgggacgt ccgggcgtct tgacccagtg ttcactggaa gaagccaccg
     9721 aactgaaact cggtatgtta ggccacacct gttacgccga aacaatttct gtctatggta
     9781 cagaacctgt tttcaccaat ggtgatgata ccccgtggtc aaaagggttt ctcgcgtcat
     9841 cctatgcttc tcgtggctta aaaatgcgct ttacttccgg ctccggctct gaagtacaga
     9901 tgggttacgc ggaaggcaaa tcaatgcttt acctcgaggc tcgttgcatt tacatcacca
     9961 aagccgcagg cgttcagggt ctgcagaatg gttcggtaag ctgcatcggt gttccatcag
    10021 ctgtgccatc aggcatccgc gcagtattag cggaaaactt aatttgctca tcgctggatc
    10081 tggaatgcgc ctccagtaat gaccagacct ttacccactc ggatatgcgc cgtaccgcac
    10141 gtttcctgat gcaatttctg ccaggcaccg actttatctc ttccggtttt tccgctgtgc
    10201 cgaactacga caacatgttt gctggttcaa acgaagatgc tgaagacttc gatgattaca
    10261 acgtcattca gcgtgacctg aaggttgatg gtgggctgcg accagtacgc gaagaagatg
    10321 ttatcgctat ccgcaataaa gccgctcgcg ctttacaggc tgtctttgcc ggtatgggac
    10381 taccgcctat taccgatgaa gaagtcgaag cggcaaccta tgcccacggt tcaaaagata
    10441 tgcctgagcg caatattgtg gaagacatca agttcgccca ggagattatc aataaaaacc
    10501 ggaacagcct ggaagtggtg aaagccttgg cgcaaggcag ttttaccgat gtcgcccagg
    10561 acatgctcaa catgcaaaaa gcaaaactga ccggcgatta tctccatacc tcagccatca
    10621 tcgtcgatga cggacaagtg ctctctgcgg tcaatgacgt caatgattac gccggaccgg
    10681 ctacaggtta ccgcctgcaa ggggaacgct gggaagagat caaaaatatt cccggtgcac
    10741 ttgatcccaa cgaaattgac taagggtgag aaatggaaat taacgaaaaa ttactgcgtc
    10801 agattatcga agacgtgctg gcagaaatgc aacccagcga caagtccgtt tcatttcgcg
    10861 cacctgtctc tgcaactgtc ccttccgcgc cagataccgg aaattttctg accgaaattg
    10921 gcgaagccca acaaggcact cagcaagatg aagtcattat tgctgtaggc ccggcgttcg
    10981 gcctggcgca aaccgtcaat atcatcggta ttccgcataa aaacatcttg cgcgaagtga
    11041 ttgccggtat cgaagaagaa ggcattaaag cccgtgtaat tcgctgcttt aaatcgtccg
    11101 atgtcgcatt cgtagcagtc gaaggcgatc gtcttagtgg ttcaggtatt gctatcggta
    11161 ttcagtcgaa aggcacaacg gttatccacc agcagggact tcctcccttg tctaacctgg
    11221 aacttttccc acaagcgcct ttgttgacgc tggatactta tcgtcagatt ggcaaaaacg
    11281 ccgcgcgtta tgcgaagcgc gagtctccac aaccggtgcc gaccctcaac gatcaaatgg
    11341 cgcggccgaa ataccaggcc aaatctgcca ttttgcatat taaagagacg aagtacgtgg
    11401 tcactggcaa aaaaccgcaa gaactgcgcg tgacgtttta acaaaggaaa ccttcatgaa
    11461 taccgatgca attgagtcga tggtcaggga cgtgttgaac cgcatgaaca gtttacagga
    11521 tgcagctcct gtttctgcgg tccccaacgc atcaatactg agcgccaaag tgacggatta
    11581 cccgctggca aataaacatc cggaatgggt taaaacagcg accaataaaa cgctcgacga
    11641 tttcactctg gaaaatgtgc taagcgataa cgtcaccgcc ctggatatgc gcattacccc
    11701 tgaaacattg cgtattcagg ctgcgattgc cagagatgct ggtcgcgatc gtctggcgat
    11761 gaattttgaa cgcgccgctg agcttacctc agtaccagat gatcgcatcc tcgaaatcta
    11821 caatgcgtta cgcccttatc gttcaactaa gcaagaactt attgccattg ctgacgatct
    11881 tgaacagcgt tatcaggcca aaatttgtgc tgcatttgtc cgtgaagctg cggaacttta
    11941 tgtcgagcgc aaaaaactga aaggcgacga ttaaggacga actatgcgat atatagcagg
    12001 cattgatatc ggcaactcat caacagaggt tgcgctggca acgctcagtg caactggaga
    12061 gttgtcgttc gtgagtagcg ccctggagga aaccaccgga atcaaaggta cgttgcgtaa
    12121 cgtccatggt attcaggagg cgctcgcaca ggcaacgaaa aaagtcggca tcaatgttag
    12181 cgatatttcg cttattcgta tcaacgaagc aactccggtc attggtgatg tggcgatgga
    12241 aaccatcact gaaactatca tcaccgaatc aaccatgatc ggtcacaacc cgaaaactcc
    12301 gggcggagtg gggctgggcg tagggttcac catcactccg caagagttat taacacgccc
    12361 ggcggacaca ccttacatcc tggtggtgtc atcggcattt gattttgccg atatcgcgac
    12421 gatgataaac gcctccgtac gcgccggata tcagctgact ggcgttattc tgcaacgcga
    12481 cgatggcgta ctggtcaata accgcctgga aattccttta cctattgtgg atgaagtcct
    12541 ctacatcgat cgtattccgc tcggcatgct ggcagcaatt gaagttgccg ttcccgggaa
    12601 agttatcgaa accctgtcta acccgtatgg gattgccacc gtattcgccc tcaatgctga
    12661 agagactaaa aatattgttc ctgtcgcgcg ggcgctaatc ggcaaccgtt cagcagtggt
    12721 agtgaaaacg ccctctggcg acgttaaagc ccgttcgata cccgccggaa atatcgaact
    12781 gctttctgcc gggcgcacaa cgcgcgttga tgtagctgct ggtgccgatg ccattatgaa
    12841 agcggtcggt gagtgcccaa aactggaaaa cgtgacggga gaaccaggca ccaacatcgg
    12901 cggaatgctg gaacatgttc gccagaccat ggcggaactg accaacaaac ccagcaacga
    12961 aatttttatt caggatttgc tggcgataga tacctctgtc cccgtcagcg taaccggtgg
    13021 tctggcaggt gaattttcac tggaacaggc tgtgggtatt gcctctatgg tgaaatccga
    13081 ccgtctgcaa atggcgatga tcgccagtga aattaagcaa aaactgcatg ttgatgttca
    13141 ggtcggaggc gcagaagcag aagccgccat tcagggggcc ttgactaccc ctggaaccac
    13201 acgtccactg gcaattctgg acttaggtgc aggctctacc gatgcctcca tcatcaatca
    13261 gagtggggaa atcgttgcca ctcaccttgc gggagcgggc gacatggtaa cgatgattat
    13321 cgctcgtgag ctgggcttaa atgatcgtta tctggcagaa gaaatcaaaa agtacccgtt
    13381 agcaaaagta gaaagcctgt tccacttacg ccatgaagac ggtagcgttc agttcttccc
    13441 tactccccta tccccccatg tatttgcccg cgtctgtgta gtgaaacccg acgaactggt
    13501 gcctattccc ggtgatttga cgctggaaaa agtacgcgct gttcgtcgca gtgcgaaaga
    13561 gcgtgtcttc gtaaccaatg ccctgcgcgc gctacgtcag gtaagccctg ccggaaatat
    13621 tcgcgacatt ccgttcgtcg ttctggtggg tggttcttcg ctggatttcg aagtaccgca
    13681 attggtcacc gacgcgctgg cacattatcg actggtcgcc gggcggggga atatccgtgg
    13741 tagcgaaggt ccgcgcaacg cggtagctac tgggctactc atcgcctggc acaaggagtc
    13801 gattcatgga aagtaaagag cgtgcaccag ccatcgttgt catggagatc ggtgactgta
    13861 tcactacatg ggatgaggtg cttcttggca ttgaagagga aggtattccc tttcgcattc
    13921 aacacattcc gtcaggtgaa gttatcgata gcgcctggct ggctgctcgt caatctcccc
    13981 tgttggttgg cattgcctgc gaccgggaag aactgattgt gcattacaaa aacttaccgg
    14041 catcagcgcc actgtttacg ctgacgtatc aacaagataa ccatacccgg cgcagcatcg
    14101 gcactaacgc tgcccgcctg gtaaaaggaa ttcccttccg ggaattccac tcataatcca
    14161 caggagaaac gctgtatgaa caacgcacta ggactggttg aaacaaaagg gttagttggc
    14221 gcaattgaag ctgcagatgc catggttaaa tccgccaacg tgcagctggt tggctatgaa
    14281 aagattggtt cgggtctgat tacggtgatg gtccgcggtg atgttggcgc agtaaaggcc
    14341 gccgttgatg cggggagcgc tgcggcaagc gccgttggcg aagtgaaatc ctgccatgtc
    14401 atcccgcgcc cacacagtga tgttgaggct attttaccta aatcggcata accccatttg
    14461 ctaaggagca cagcgtgaag caatcactgg gattacttga agccagtggt ctggctctcg
    14521 ccatcagttg cgcagacgct atggcaaaag ccgcgtccat cacgctggtt ggccttgaga
    14581 agaccaatgg ctcaggctgg acgctcatca aaatcaccgg tgacgtcgcc tcggttaatg
    14641 cggcgatcgc gacgggagcc agtttcgccg gacagcgaaa tggccttgtc gcacacaaag
    14701 tgattgcccg cccgggagac ggcattctgc cccacacaac tgaagcattg accgtgacgg
    14761 ttgaaacaag caaaacacca gcaacaaccg tcacctcaga gattatcagt tgtaatttgt
    14821 gtcttgaccc ggcatgtcag cggcaaaaag gtgagccccg ctcactttgc ctgtacgcca
    14881 ataagcgagg ggagtagcag tggataaaca aaccttgcag gaaacagtga gccagattct
    14941 ggatgagatg cgcgagcgcc ctatcccact gggcgtttcc agtcgccata tacatctttc
    15001 atcagcggat tatttgcgtc tttttcctga acaaccgatt agcgagaaaa agccgttaat
    15061 gcaacctggc caatacgctg ctgaacaggt tgttacgctg gagggaccaa aaggcaaatt
    15121 gcacaacgtt cgcttgttgg ggccgctacg caatgcaagc caggtggaaa tttcccggac
    15181 ggacgccaga acgttaggga tcaccgcccc gctacgcatg tcaggcgatc tcagcggcac
    15241 tccggggatt cgtttgataa gcccatttgc ggaactggag atcacatctg gcgtcattgt
    15301 cgcgcaacgg catattcata tgtctccgct ggatgccctg atttatggcg ttacgcatgg
    15361 cgataaagtg gcggttgcca ttgaaggtga tgagcgacga ctgatattcg aaaacgtcgc
    15421 tgttcgcgtc tctccggata tgcgtcttga aatgcatatt gataccgatg aggccaacgc
    15481 cgcaggcgct gatagccctg acgtttttgc cacactggtg tcagtgccat gaataacatg
    15541 ctgatgcaac gtattgttga cgaagtggtt tttcgcctta aacaacgtgc gggaaaaaca
    15601 ctcgtgctga ctgtttttca attgcgagac gccagcgttc aagagagcgt tcatcagtat
    15661 gcgtcattac aaatccgata tgtcgatctg ccgctgctgc gacaacttgc ggagaacgaa
    15721 acgtcagatc gagcatctat ccagatacac gaagcgttag cctggggaat gcacattcaa
    15781 ttgtcgttgc agcgtcattt cctgaacgcc atcgaattga agactctcgc ccgtctaccg
    15841 ctaagctggt gcgacgaaca agggcagccg atctaccttc atcataatcg acttttaagt
    15901 tatgccgata tcgcacagtt aagctcggga atactggtgt tacagcggaa atgttgtgtg
    15961 accgcactgg ctcgtgaagc ggcaattacc cgaaatattc agttaatcag gcaggagtaa
    16021 cctatgcatc tggctcgagt cactggcgcg gtagtatcca cgcaaaaatc accttctctg
    16081 atcgggaaaa aactgctgct ggttcgccgg gtcagtgctg acggcgaact gcctctcacg
    16141 ccattaaccg gcgacgaagt tgccgttgat tcagtcggcg caggcgttgg tgagttggta
    16201 ctcctcagca gtggttccag cgccagacat gtgttttcag gccctaacga ggccatcgac
    16261 ctggcagtgg tcggcattgt cgatacgctt tcgcgctaag gagctaaaag atggcaattt
    16321 atacccgaac aggtgattca ggaagtacgt ctttatttac cggacagcgc gtcagcaaaa
    16381 ctcatctacg tgtcgaaacc tatggcacgc tggatgaact gaacgcgaca ctcagtctgt
    16441 gttattgcgc caccgccatc gaaagccatc gcatcttact ggaagccatt caacaacaga
    16501 tattttggtt tagcgccgaa ctcgccagtg aaagcgaaca gccgtcagcg caacagcgtt
    16561 acatcggtac ggaagaaatt gccgcgttgg aaaacgcgat cgatagtgca atgaacgccg
    16621 tccccccagt tcacagcttc attctgcctg gtcgatgtga agccgcaagc cgcatgcatt
    16681 tcgcccgcac ggtggctcga cgggctgaaa gacggctggt tgaactgaca acggaaacca
    16741 ccgtccggaa tgttttgctg cactatatca accgactttc cgattgcctg tatgccctcg
    16801 cccgcgtgga agacaacgtt gctcatcaaa acctgatgat tcaggaaatc acaaaacgtt
    16861 atcacgaagc caaccacaca ccagcattga aggaacgcac aatgccgctc acttttcagg
    16921 atcttcacca gctcattcgt agtgcggcaa tgcgcgcgga tgaactgcat attcccgttg
    16981 tcatcagcat tgttgatgcc aacggaacag aaagtgttac ctggcggatg cctgatgccc
    17041 tgttggtcag cagtgaactg gcaccgaaaa aagcctggac cgccgtggcg atgaaaacgg
    17101 caacccacaa gcttgctgat accgtgcaac ccgacgcgcc gctttacggg ctcgaaagcc
    17161 atatgcaagg aaaagtcgtc acttttggtg gcggcttccc cctctggcgt gacggaaaat
    17221 tgcttggcgg gcttggcatc agcggcggta gcgttgaaca agacatggat attgcccaaa
    17281 gcgcaatggc ggcaattaac gtgggagtaa accaatgaat aactctgaat tagaaacact
    17341 gatccgcaat attctgcgtg agcaacttgt ccccgctaca ccacgaacgc aaagaaacgc
    17401 tattttccag actgtcgatg aagcggtctg cgcagcacac taggcctttt tacgctttca
    17461 acagtgcccg cttaaaaccc gtagcgccat tatcagcgcc attcgcgaag aattaatgcc
    17521 ccgccttacc gaactggcag aagaaagcgc caaagaaacg gggatgggaa ataaagaaga
    17581 taagtaccta aaaaacaaag cggcactcga taacacgcca ggaattgaag atttaaccac
    17641 cacagcacta accggtgatg gtggcatggt tttgtttgag tactctccgt ttggggtgat
    17701 tggctcggtc acgccaagca ccaaccccac ggaaaccatc attaacaaca gcatcagcat
    17761 gctggcagca ggtaatagcg tctacttcag tccacacccg ggtgccaaaa acgtttcgtt
    17821 aaaactgatt accacaatcg aagacatcgc gtttcgctgt agtggcattc ataacttgat
    17881 cgttaccgta tctgaaccga cctttgctgc caccgaacag atgatggcgc accctaaaat
    17941 cagcgttctg gcgattaccg gcggtccggg gatcgtcgcc atgggaatga aaagtggcaa
    18001 gaaagtgatt ggtgccggag cgggtaaccc tccgtgcatc gtcgatgaaa cggcggatat
    18061 tgtgaaagcc gccgaggaca tcatcaacgg cgcttcattt gattacaacc tgccgtgtat
    18121 tgctgaaaaa agcctgattg tcgtggcatc agtggcagat tatttaattc agcagatgca
    18181 aagttttggc gcacttcttc ttaactatga gcaaactgag aaattgcgtg caatttgcct
    18241 gccagacggt agcgccaaca aaaaactggt tgggaaaagc ccatcagcct tgctggaagc
    18301 ggcaggcctc cctttgcccg caaaagcgcc gcgcctactg attgctgttg tcgacgccga
    18361 tgatagttgg gttacttgtg agcaactaat gccaatgctg cccattgtga aggtcaagga
    18421 ttttgacagc gctctaaccc tggcgctgaa agtggaagac ggcttgcacc acacggcaat
    18481 tatgcactct cagaacgtct cacgactcaa ccttgccgcg cgcgttatgc aaacctcaat
    18541 atttgtcaaa aacggcccct cctgggcggg tattggcgtc ggcggcgaag gctttacgac
    18601 ctttaccatt gcaacaccga ctggagaggg tacaacgtca gcacggactt ttgcccgctc
    18661 acgacgctgc gtgttaacca gcggcttttc aattcgctag agaaacggaa atgaatactt
    18721 tttcactgca gacgcgttta tacagcggac caggaagtct caaggtactt agccgtttct
    18781 ctcatagaca catctggatt atttgcgatg cctttctggc acgctcacca cttattgaca
    18841 cgttgcatca ggcgctgccc gacagtaatc gtctcagtat ttttagcgaa ataacgcccg
    18901 ccccaactat ccagaccgtg gtgaaaggca ttgcgcaaat gcagacttta cgcccggatg
    18961 tagttatcgg ttttggcggc ggttctgccc tggacgccgc aaaagcaatt gtctggttcg
    19021 gtcgccagtg tggcattgaa attgaaacct gcgtagccat tcctaccacc agcggtatcg
    19081 gttccgaagt gaccagtgct tgtgtcatca gcgatcctga gaaagggatt aaatatcccc
    19141 ttttcgataa tgcactttat ccggatattg cgatccttga tccatctttg gtcgtcagcg
    19201 tgccaccggc gataacggcc aataccggga tggatgtttt aacccatgcg cttgaagctt
    19261 atgtttctcc tcgcgccagc gacttcacgg atgcactggt cgaaaaagcc gtgcaaattg
    19321 tttttcaata cctgcccact gcagtgaaaa aaggcgattg tcttgccacc cgtggcaaaa
    19381 tgcataatgc atcgacgctt gcaggaatgg cctttagcca ggccggactc ggtttaaacc
    19441 atgcaattgc tcatcaactc ggcggacagt ttcatctgcc gcatggactg gcgaatgcgc
    19501 tcctgctcac ggcagttatc cgctttaatg ctggcgatcc gcgcacagcc aaacgctatg
    19561 cccggctggc gaaaacgtgc catttatgcc cggacaacgc caatgacacc gccagcctga
    19621 atgcacttat tcaacatatt gagcagctta atacaacctg tacgctgccg actcttgcca
    19681 atgcgctgaa agaaaaaaaa gcagaatggt caatacgcat accagatatg gttcaggcgg
    19741 cgctggcgga tgcaacgttg cgtaccaacc cgcgtgcagc tgatgcctcc gcgattgcag
    19801 aactgctcaa ggggttgtta tgaacgtcgg attaatctcc tgcgacgacc agaccatccg
    19861 cgagcgagta caagcagcag gtgtcgtggg tgctggcggc gcaggttttc caacccatat
    19921 taagctacaa gcgcgtgctg acactttcct ggtgaatgcg gcggaatgcg aacccatgtt
    19981 gaaagtagat caacagttaa tggcgcagca agccagccgc ctgcttcgcg gtgttcacta
    20041 tgcgatgaaa gccaccgggg ccagtagcgg aatcatcgcg ctgaaagaaa aatatcaacg
    20101 ggcaataaac gcactcacac cactgttacc accggatatc cgcctgcata ttttgcccga
    20161 cgtttacccc gcaggagacg aagtgctgac catctggatg gcaactggtc gccgcgttcc
    20221 acccgctgca ttacccgtta gcgtcggtgt ggtggtcaat aatgttcaaa ccgtacttaa
    20281 tattgccaga gccgtcgagc aacagtatcc agtgacgcgc cgcacactca ccgttaatgg
    20341 tgcagtggcg aagccaataa cggtaaccgt ccccatcggc atgtctttac gtgaagtcct
    20401 ggcgctggcg ggcggcgcaa cagtggatgc tcctggtttt atcaatggcg ggccgatgat
    20461 gggaagtttg atcacatcgc tcgacacacc agtcagcaaa accactggcg gcttgctggt
    20521 tttacccaac tctcattcac tgatccaacg tcgaatgcag aatgaccgca gcgtacttgc
    20581 cgtcgcgaaa actgtttgtg agcaatgccg cttgtgtacc gatctctgcc cacgtcattt
    20641 aatcggtcac gaactggcac cccatttatt agtacgtgcg cggttaacta ccaacagctc
    20701 gccacgccgc aactgttact gacagcgctc acctgttcag agtgcaacgt ctgcgccagt
    20761 gtcgcctgtc cggtaggaat ttcaccaatg cgcataaacc gcctgcttaa gcaggaatta
    20821 cgcgcacaaa atctccgcta cgaaggtgcc ttaaatcctg cagatcccat ggcaaattat
    20881 cgactgattc ccgtaaaacg gctggtaacc agacttggct taaccccgtg gtatcaggat
    20941 gcgccattgt ctgaacaggt gcctcagccc gaaaaagtga cgttattgtt acgccagcac
    21001 atcggtgcca gcgcaattgc gtgtgtacaa aaaggcgatc gcgtcgttca tgggcaatgt
    21061 gtaggtcaaa ttccacacgg cacattaggt gcccccattc atgccagtat tgatggcatg
    21121 gtcagcgatg tgacagaaaa cgcgattacg cttgtgagag gttaaccatg tcgaaagcgg
    21181 taggtatttt agaacttagc agtatcgcga aagggatgga aaccggcgac gccatgctga
    21241 aaagcgccaa cgttgagtta ctggtcagca aaacgctatg ccccggaaaa tttctgctga
    21301 tgctcggtgg cgatgtgggc gcggtgcaac aggccatcaa cagcggagcg tcccttgctg
    21361 gtgaaatgct ggtcgacagt cttgtcctgc cgaatattca tcccagcctc ctttctgcaa
    21421 tcagcggact taaccatgtt gaacagcacc gggctgtcgg cgtagtcgaa acctggagtg
    21481 tcgccgcctg cattgacgct gccgattgtg ctgtcaaagc tgcaaatgtc attcttgttc
    21541 gcgtacatat ggcttttggc attggcggta aatgctacat ggtggttgct ggcgatattt
    21601 cagatgttaa taacgcggtc tctgccgcca gcgtgcgcgc gggggaaaaa ggtttgttgg
    21661 tctatcggtc ggtgattgcc cgtccacatg aagccatgtg gcgtcagatg gtggagggat
    21721 aatgacaact cagcacacaa ctgaacggat gattcaggaa tatgtcccag gtaagcaggt
    21781 cacgctggca catgtcatcg ccaatccagg gaaggatctg tttaagaaat tgggtttacc
    21841 agacaccatt tccgctattg gtattctgac aattaccccc agcgaagcat ctatcatcgc
    21901 atgcgatatc gccacaaaat caggcgctgt agagatcggt tttcttgatc gttttaccgg
    21961 tgccgtagtg ctgactggtg atgtttctgc gatcgaatat gcacttagcc aggttacccg
    22021 tacgctgggt gaaatgatgc gctttaccgc ctgccccatt acgcggacct gacgttatgc
    22081 aacgtatgat gctcatcggc cccagccagt gcggaaaaac gtcactcacg caatgtctgc
    22141 gtggcgaaaa tattcattat cagaaaacgc aggcaatcgt ctggtccccg accaccattg
    22201 atacaccagg cgaatacctc gaaaaccgca gcctctacag tgcactgttg gtcagtgcct
    22261 gcgaagcgga cattatcgcg ctggtgctta atgcggacgc accgtggtca cctttttcgc
    22321 caggctttac ctcgtcaatg aatcgcccgg tcattggagt agtaaccaaa gcagatttag
    22381 ccaacatgga acaaatatct ttggtgaaat gttggttacg ggaagctgga gcgcacaacg
    22441 tgttagtaac cagtgcggtt aataaccatg gagttgcaga acttttagcc ttgctgcata
    22501 cagaagaagg ctgtcgctaa tttcgacagg ttatcagatg ggaatgatga cggtgataca
    22561 cagaaaagaa tcaatgagca atgagggttg ccgggcaacc ctcattgaat aaaacaggaa
    22621 ttacttcgct tcgccgtttt tcatttcgcc catagctttc agctttttgg agatatcgcg
    22681 gcgttctttg gagagctcgg catttttgat gatgtaatcg tcaacgcgat cttcatagtc
    22741 acctttcatg ctggcgatga tgccctggat tgcttcaacg ctcatcccag gcttgatgta
    22801 atcgctcagg ttgtccagca gcaggacgcg cttctggttg tcacggatct ttttctcaac
    22861 gtcctgaatt tcacgttgca gtttgttctt acgacggaac agacgaacaa attccagtac
    22921 gtcctggaat gaaggcttgg tagtttccat ttttataccc ctgataatgt gagagtcgga
    22981 ttcgtttaag caaccgctat tcgttagggc caacattact catagctgcc gcagatgaca
    23041 atgcttttat cccttttcac tatcataccc tttatccttg ctgaatcgaa gcagcagcaa
    23101 gatgattctg aagttcagga attatttgcg caaggcccgg caaatcagat tcgatagcaa
    23161 ctcaagctga tgcgccgttt ccatgttcag ccacacataa ccatagattg gtgtttccac
    23221 taccttcagg tcatggtggt tattgagcaa ctgacgcagc tcttccacag catcgttcaa
    23281 tttttccgta ttggcaaaaa ccggctgcgg attaccttcg tacagcgcat gaacaaagct
    23341 cagcagtatt tgctgcatca tgtgctgggt atcacgaagt ttttgcgcgt tcaataacac
    23401 gaaatggctg gggcgcgtgg cccagtacgc attgatttgc aactccagca tacaaaccag
    23461 attgcggtta atggtctgga taccttcata tatcgatttt ggaatacggg tttctttgct
    23521 ggcgggcgca atcagcccac gcattttcac ggcatcggtc aggagttttt gtagatggct
    23581 ttccagacgt gggcgttcga gtaagttcgg tgagaatgca gattgataga cccgattata
    23641 ctcggtcaga cttttcgcca gttgaatgcg ccagtggatg aacgcccgtt gtggccagat
    23701 accggtaaac aacattgcca gtaaagagcc gaggatcaca tcgccgcttc gccataacgc
    23761 cgtatcaatt tcacctgtcg gggaaccaac aacaattgcc agcgtcaccc caatcaataa
    23821 tccttgatac ggtttcttgc ccagcgccag ccaaccgcaa agaaacatcg ccgccgcgca
    23881 ccagactaac atcagcggta acgaaattaa ctccagttgc agagcgataa gacctaaaat
    23941 cgaacccaac accgtaccgc caatacgttc gaaggcacga gggacaacgt taccccagaa
    24001 cgagattggc cccataatca ccaccatggt gaccagcggc caggtgcttt ccgggatagt
    24061 aaacaggcgg ataatgagaa aagtgagcag gaatgccagc gcgacccgag taccatgcac
    24121 tatgcggtaa tggcggtata cccggatttc aaacgggctt aatgacttat cggcacgcac
    24181 gcagaaactc cgtaatgaag attaaaagcg ctcataactc atctgtcggc cagaaggcgc
    24241 gcctctgaat tatccgacgg acaaaccggg aacagtgtaa tttcccggca ggagcgaaaa
    24301 aattgaaagg cgcatcttac tcttttcgct ttcaaaaaaa gagtggtcat cgcgttaaca
    24361 caccgccctg agatgaatta gtgatgtttt ggctgcaccg caacatacat ttcgatatcc
    24421 cagtacccat cttccgcgcc attgttcaaa taaacctcga agcagggctt tggtaacatt
    24481 tcataagcac tgtcctgcaa gaggctatta aagaactgat accagggttt agcaaaatca
    24541 tcaccgacta cacgagccac cgccaccgca tactgaccac ctgaaatttc tgtcagaatg
    24601 acgccctcac tgttttcggg aagcgtaaag ttattcggta ccgtcacaac ggtgtcgcag
    24661 cgtaattttt cggcgggtgt ttcatctgga ttgtcgtaat agacagcaac ccactccttc
    24721 ggcacaatat ttttgttatc tacccacatc atcaactgct caaagccttt ctttaccgtc
    24781 tgttcccacg ggccaacgag atggaaacct gcaacggtac gtttctcttc ctgcgtaatc
    24841 tcgtagttca tgacgcctcc attaacactg tttttatata cagcatagtt gcaatattaa
    24901 aaccacaagg aatgagtgtt gattatgcga gcagactcgc actcctgcca gtctgctgca
    24961 aaagaaaggt caggccttat ggtggaaata atcactcagg cgggaaaaca tgccgccttc
    25021 cccgacagat tccagggtaa ccagcggcca gtgcgccacc tgtttatcac ggtcgtaaag
    25081 ttcaatttcc cctacccgct gatgggcgct aattggcgcg gtgagctctt taccatcaag
    25141 ggtatatttg gctttgatat gtggaatttc ggctttcggt agcaccatcc agaactcttg
    25201 ttccgttcct aggtcgatat tttctttatc gccataccag atgcgttccg ttccgacctt
    25261 tttcccacgg tgcaaaattt gcaccgtagt aaagttttgt tgcccccaac gcagtaattt
    25321 tcttgcctct tcctcacgac cttttgcact gtcagccccc attaccactg caatgagacg
    25381 acgctgccca tctacagccg aagcaatgag attaaacccg gcaccagaag tatgacccgt
    25441 tttcaggccg tcaacattca tggttttatc ccacaacaac ccgttacggt tttgctgggt
    25501 gataccgttc caggtgagac ttttctcact gtacatatga taaaactcgg gctcgccgtg
    25561 gatgatagcg cgagaaaaca cagctaaatc ataagccgag ctatgctggc caggtgcatc
    25621 cagaccatgc actgtttcaa aatgcgtatc cttgagatgc agcttctcgg catagttgtt
    25681 catcatttca acaaactgcc gttgcccacc ggcaatatag tcagccagag caacacaagc
    25741 gtcatttccg gaatccacaa ttaaaccacg gcttaaatca cgtaccgata cgcgatcgcc
    25801 ctctttcaaa aacatcagtg aagaaccgac aaacaccgga ttatctttcg cccacgcatc
    25861 gcgccccacg gtgacaatat cgtctggcgt aatgcgatga ctatcgatag cgcgatccac
    25921 gacataaccc gtcatcagct ttgtcaggct ggcgggattg cgctgttgat gctcattacc
    25981 cgcggtgagg atctgaccgg tggtgtaatc catcaatacc caggacccgg catgaatctc
    26041 tggaggctga ggtgaaaaag gaatgttttc cgccgcaaaa ccagacgata agttaaaaac
    26101 gaacaaagaa gcagcaataa taagacggcg tttcaacagc aaaccctcag gagtttcaaa
    26161 tagctgttct ttttacggaa atacttatga actggctgga ataaagtgca agaaaatgtg
    26221 actaccctct catttttatc tgacatgatc tgttgccact cgctgccaaa ttgtggcgct
    26281 aaagctgatt agcacggtga tatttgatac tctggcagac agcagaaata acggatttaa
    26341 cctaatgatg aatgacggta agcaacaatc tacctttttg tttcacgatt acgaaacctt
    26401 tggcacgcac cccgcgttag atcgccctgc acagttcgca gccattcgca ccgataacga
    26461 attcaatgtc atcggcgaac ccgaagtctt ttactgcaag cccgctgatg actatttacc
    26521 ccagccagga gccgtattaa ttaccggtat taccccgcag gaagcacggg cgaaaggaga
    26581 aaacgaagcc gcgtttgccg cccgtattca ctcgcttttt accgtaccga agacctgtat
    26641 tctgggctac aacaatgtgc gtttcgacga cgaagtcaca cgcaacgttt tttatcgtaa
    26701 tttctacgat ccttacgcct ggagctggca gcatgataac tcgcgctggg atttgctgga
    26761 tgttatgcgt gcctgttatg ccctgcgccc ggaaggaata aactggcctg aaaatgatga
    26821 cggtctaccg agctttcgcc ttgagcattt aaccaaagcg aatggaattg aacatagcaa
    26881 cgcccacgat gcgatggctg atgtgtacgc cactattgcg atggcgaaac tggtaaaaac
    26941 gcgtcagcca cgtctgtttg attatctctt tacccatcgt aataaacaca aactgatggc
    27001 gttgattgat gttccgcaga tgaaacccct ggttcacgtt tccggaatgt ttggggcatg
    27061 gcgcggcaat accagctggg tggcaccgct ggcgtggcat ccagaaaatc gcaatgccgt
    27121 aattatggtg gatttggcag gagacatttc gccattactg gaactggata gcgacacatt
    27181 gcgcgagcgt ttatatactg caaaagccga tcttggcgat aacgccgccg ttccggttaa
    27241 gctggtgcat atcaataaat gtccggtgct ggcccaggcg aatacgctac gcccggaaga
    27301 tgccgaccga ctgggaatta atcgtcagca ttgcctcgat aacctgaaaa ttctgcgtga
    27361 aaatccgcaa gtgcgcgaaa aagtggtggc gatattcgcg gaagccgaac cgtttacgcc
    27421 ttcagataac gtggatgcac agctttataa cggctttttc agtgacgcag atcgtgcagc
    27481 aatgaaaatt gtgctggaaa ccgagccgcg taatttaccg gcactggata tcacttttgt
    27541 cgataaacgg attgaaaagc tgttgttcaa ttatcgggca cgcaacttcc cggggacgct
    27601 ggattatgcc gagcagcaac gctggctgga gcaccgccgc caggtcttca cgcccgagtt
    27661 tttacaaggt tatgcagatg aattgcagat gctggcacaa caatatgccg ataacaaaga
    27721 gaaagtggcg ctgttaaaag cgctttggca gtacgcggaa gagatcgtct aatttgaagc
    27781 ctccgccgct ggtacggcgg aggtcaatgt tgagtcaggc tttttgaacg gtgatactcc
    27841 acgctgcatc gccaatctgc tgataatcgg tgatggcatg cccttcttct gccgcccact
    27901 gtgggattgc ttcagttgcc tgggtgcaat caaactcaat taccaactca tcgccgctgg
    27961 ccatttctgc cagtgctgct tttgcttcta tgagtggaaa cggacagacc tgcgtcacca
    28021 catccagctt tttaatcgcc atctttacac cttacttaat ttgccgcagc agttgccagt
    28081 cgcgccttac gctgcggtcg aacatacaca agccaggacg cagtccagac tccgagaatc
    28141 ataaatacaa ggccaatcca gccctgccag gtcatcattg cagtcataac cagtccatta
    28201 ccgataaagc aaccacctgc aatactggca ccgaagccca tcagtacacc acctaacccg
    28261 ctacgtaatg ttgtttgtgc atcagctgcg cgaacgcgga actcacggct cgctttggct
    28321 gcaataaatg accccacgaa gatccctaac accaggaaaa cgccccagtt aatgtatttc
    28381 acgtcacccg cgaccagaaa ttgcagaata ttggccgttg gggaagtgat tcctaaccca
    28441 aacatgcgtc cggttgcttc actcaggggc caggctaaaa gcgcaatcaa accgatgagt
    28501 acagcagtga caaagggatg ccagcgtttt tcaaacagaa tatgagcgat cccagttcgg
    28561 cgcggcggta aggtcgcgac tttgagtttt ggcttcttca actctttcat caccacccag
    28621 agcgttatca ccagcaaaac ggcaaccaac ggccaaacag acaaattaaa agtctcagca
    28681 atagagttat gttcagtact gtagtgctgc aaggtttgat ttaaaccact ggcatgtgga
    28741 gaacgcatca ccgcactcat caccatataa gtgaaaagcg cgatccaact gccgatcaat
    28801 ccctcacccg cgcgatacca ggtcccggtg gcacatccac ccgccagaac aattcccagc
    28861 ccgaagatat acccacctat aacagtacca agccacggga acgcctcggc ttcgtaagtc
    28921 agtaggcccg cctgaattaa cgcaaaaacc cccacgcttt gtaccgaaat tgcaatcagc
    28981 aaggcgtaaa acatgcgatt atttttcgcg atatacatat cgcgaaaacc acctgtcagg
    29041 caaaaacgcc cacgctgcat gacaaatccc agcagagcac cacaaattag cccgcttaat
    29101 atcattgaaa acataaatta attaaccaga tgaatgttaa tgaggaaatt attcatgact
    29161 ggtggaatgc agaccaataa ccaaattctc taaattagaa caaatggtta ttaatgagga
    29221 tttcgataaa aaaaaataaa aaaaccggag ccaaatattc attcgactcc ggttgattat
    29281 tagaaaagat gattatgcaa cgtcttcgta ctgcggaact gggttacgga agctcttggt
    29341 gacgcaagcc aggtaaatca gaccgatagc agcccaaatc agacccagaa ccattgagct
    29401 ttcttccagg ttaacccaca gcgcaccaac ggtcagcgca ccacacatcg gcaggaacag
    29461 atactggaag tgatctttca gcgtcttatt acgcttctca cggatccaga actgcgagat
    29521 gaccgacagg ttaacgaagg tgaacgccac caacgcacca aagttaatca gcgctgtagc
    29581 cattaccagg tcgaagttga ttgccagcag ggcaatcgcg ccaaccagga tgatgttcat
    29641 cgccggagta cgccatttcg ggtgtacata accgaagaag cttttcggga atacgccgtc
    29701 acgacccatt acgtacatca gacgcgctac gcctgcatgc gccgccatac cggacgccag
    29761 tacggtaatg gtggagaaga tcagcgcgcc aacctggaac gctttacccg caacgtacag
    29821 catgatttca ggctgtgacg catccggatc tttaaagcga gagatatccg ggaagtacag
    29881 ctgcaggaag taagttgcaa agatgaagat catgccgcca atcagcgcgg tcaggaaaat
    29941 cgcacgcggg atcacgcgct ctgcatcttt ggtttcttcc gacaggttgc tgatgccgtc
    30001 aaagccggta aaggaaaagc acaggattgt cgccccggta atcatcggaa taacatgcgc
    30061 atcgccagac cagaacggac gagtgctcgc cagcgtacca gcgccttcac cttcaaatac
    30121 gccataaaca accatgccca gaatcaccgc gatcagcact acctgcaaca cgacgattac
    30181 agtgttgaag ttcgctacgg atttcagact acgcaggtta aaggcggtca taaaggccac
    30241 cagcgccacc acaaacatcc acgatgggat ggaaggcacc agagcttcaa aatagatttt
    30301 cgccagcaga atgttgatca tcggcgcgaa cagatagtcg agcagagaag accaacccac
    30361 cataaagccg acagtcgggc taatggattt ctgggcgtaa gtatatgcag agccagcaga
    30421 aggatagcgg cgaaccagct tcccgtagct cagagccgta aacaggatcg caatcaatgc
    30481 gaacgcatag gctgtcggca catgaccatc cgtgaggcct gaaacgatac caaaagtatc
    30541 aaacagcgtc atcggctgca tataggcaag acccatcatt acaaccggaa ctaacgtaag
    30601 cgttttacgc aattccacgc gagaggtgtt tggagtaacg ttatgcgaca tggtcattct
    30661 cctttacggt gataaccgtc gcgtaagcaa aaaattgccc catttttttg gattcctcag
    30721 cgacaacaac tgtcgatttt tagtaaatat ctatccggta cgaagcccgg cctcttggta
    30781 tgaatgattg gtttgaagca aaaaataacc gacgctgatg aaacgtcggt ttttagtcat
    30841 tttttgacag cggcgcattg tgcctaaatg ggggggaaat gacaagagaa tgagaagctt
    30901 gtcataataa tttttcttta aatggctgat tttccgtcat cagattttgc ataaacaccg
    30961 gcgatcgcac tatttgctaa aatttcatcc cgccaccagc cgctggcaag ccctgcggtc
    31021 tgttcgttcc agccaatcca gacgggttca aagagagttt gggcgaccac tttcttctcg
    31081 attaacgcgc cactatcgag aaaacgttgc accagataac ggggtaaata gccgcagccg
    31141 aggccgctaa tttgcagctc cagcttggtt ttaaaatcga aaacggtaat ggcttcttgt
    31201 tcgtcgagaa gttgtgaggc cgtagaagcg cctgcctgcg cagtgtcccc caccacaatc
    31261 gcccgatatc gcttaatgat acgacggttt aacggctctt cttcctgggc taacggatga
    31321 tgaggcgcga ccgcaaagac ttgttccaga tcgcccaacc gcgagaaacc aaactcactg
    31381 gaagatggtg gctcatgcat cgcgccaaca ataatatccg ctcgcccctg agtcagggca
    31441 tcccaggaac caccgagtac gccattgata aacttcaggc gcgtcacgct atgatgttga
    31501 taaaaagctt caatcagtgg cgcaagcaga gaaaaaggaa acgtgtcgtc cacgccaatc
    31561 accagctcgt tttcccagcc ttcatgcagt ttgatcgcct gcttttccag ttctcgcacg
    31621 gtatgcagaa cttcccgccc cttctctaat aacatttttc cggtacgggt gaatttagcg
    31681 cggtgaccgc tacgatcaag taattgaata ttaaggtcgc tttccagctt gtgaacggtg
    31741 taactcagag cggacggcgt cttgtagagt ttagccgacg ccgccgcaaa actgccttct
    31801 ttttctaagg catcgaggat catcaacaca tccagcagtt gtttcataca cgcccccttg
    31861 cagtgcatgc ggcgtcctgc tggcgtgatc gtttactcca gcggcattac cagcggatcg
    31921 ggatactggt attcaaatcc cagttcatta caaatccgac tgccatcaat aatcttacct
    31981 ttgccgctgt ccagactatt tctgaactgc ggaggttcca gccccagtaa acgggccatc
    32041 tgcggataga aaacattacg cgcagggtga gctggcgcac atatattata gatgtgtccg
    32101 cctttaggtg cctgtaacaa cagagtgata gcgccaataa catcttctaa atggactaaa
    32161 ttaacaccat gttcaccatc aggcgcggtt tttccggcaa agaagcgtcc gggatgacgt
    32221 cccggtccga ccagccccgc aagacgcaga atatcgaccg aagtaccggg taaattgtgc
    32281 agccagtctt cgagttcttc taatactcgt ccactgttgg tcaccggatt acgcggggtg
    32341 gtttctttca ccgtgccttg cgcgtcgcca tagacagacg ttgagctggt aaaaataatg
    32401 cgaggaatac gatgggccag cgcgctatcc actaactctt gtaccgcttg taaatagaac
    32461 tcatcgccgg ggccgctacg acgtgccgga agcgtaatga ccagcgcatc ggcatccatc
    32521 agggcatcca gatcgtcaga atcgcaaact aactcaggct ccatgcgaag cagatagcta
    32581 tcaatgccac tcattcgggc cgcttcgaca ccatcttgtg tggttttact cccggtgact
    32641 tgccagcctc gcgctgaaag tgacatcgcc agcggcatgc ctaaccaccc taacccgaca
    32701 attgcgacct ttttcatccg ttatctcctg atacacctta gatctataag gctacgctag
    32761 cgtatcaaaa ctgacaattc attctatgaa tgaatctgtt caataatgat aacgacatgc
    32821 tgcaatgagc agtgaatcgt cggtaaccgc gtataccaga cggtgctcct ctgtaatgcg
    32881 tcgggaccag aaacctgaca aattatgttt caggggttct ggcttcccct taccttcaaa
    32941 tggcgttctg cgggtatctt tgataagttc attgatcttt ttaacaattc gcttatctgt
    33001 ttcctgccag tacagataat catcccatga ttcctcagac cagattagtt tcactcaata
    33061 atgtcctttt ctgttccttt gcctgatttc aggctatcga ttgagtccat caatctccgg
    33121 gcgttagcgg gggagcgcag tagataagcc gtctcttcca gcgagttgta ttcttcgagt
    33181 gacatcagaa cacaagcctc tccattctga cgagtaataa ggatcggggc atgatcttca
    33241 acggctttca tcattgttgc cgacaaattc tgacgcgctt cgctgtagct aattgtacgc
    33301 atgtcaatct cctcttttgt acagtttatt gtacaatgat gggcgttaat taactattta
    33361 ttaattagtt tgtagatcaa ggtattgtca gtgagacgaa aatccaggct tcgctatttt
    33421 tggtgccgtc agcttagagg acagtcctct tagccccctc ctttccccgc tcattcatta
    33481 aacaaatcca ttgccataaa atatataaaa aagcccttgc tttctaacgt gaaagtggtt
    33541 taggttaaaa gacatcagtt gaataaacat tcacagagac ttttatgaca cgcgttcaat
    33601 ttaaacacca ccatcatcac catcatcctg actagtcttt caggcgatgt gtgctggaag
    33661 acattcagat cttccagtgg tgcatgaacg catgagaaag cccccggaag atcaccttcc
    33721 gggggctttt ttattgcgcg gttgataacg gttcagacag gtttaaagag gaataacaaa
    33781 atgacagaca acactcgttt acgcatagct atgcagaaat ccggccgttt aagtgatgac
    33841 tcacgcgaat tactggcgcg ctgtggcatt aaaattaatc ttcacaccca gcgcctgatc
    33901 gcgatggcag aaaacatgcc gattgatatt ctgcgcgtgc gtgacgacga cattcccggt
    33961 ctggtaatgg atggcgtggt agaccttggg attatcggcg aaaacgtgct ggaagaagag
    34021 ctgcttaacc gccgcgccca gggtgaagat ccgcgctact ttaccttgcg tcgtctggat
    34081 ttcggcggct gccgcctttc gctggcaacg ccggttgatg aagcctggga cggcccgctc
    34141 tccttaaacg gtaaacgtat cgccacctct tatcctcacc tgctcaagcg ttatctcgac
    34201 cagaaaggca tctcttttaa atcctgctta ctgaacggtt ctgttgaagt cgccccgcgt
    34261 gccggactgg cggatgcgat ttgcgatctg gtttccaccg gtgccacgct ggaagcaaac
    34321 ggcctgcgcg aagtcgaagt tatctaccgc tcgaaagcct gccttatcca gcgcgatggt
    34381 gaaatggaag aatccaaaca gcagctgatc gacaaactgc tgacccgtat tcagggtgtg
    34441 atccaggcgc gcgaatcaaa atacatcatg atgcacgcac cgaccgaacg tctggatgaa
    34501 gtcatcgccc tgctgccagg tgccgaacgc ccaaccattc tgccgctggc gggtgaccaa
    34561 caacgcgtag cgatgcacat ggtcagtagc gaaaccctgt tctgggaaac gatggaaaaa
    34621 ctgaaagcgc tgggtgccag ttcaattctg gtcctgccga ttgagaagat gatggagtga
    34681 tcgccatgag ctttaacaca atcattgact ggaatagctg tactgcggag caacaacgcc
    34741 agctgttaat gcgcccggcg atctccgctt ctgaaagcat tacccgcact gttaacgata
    34801 ttctcgataa cgtgaaagca cgcggtgatg acgccctgcg ggaatacagc gcgaagtttg
    34861 ataaaaccac ggttaacgcg ctgaaggtgt ctgctgagga gattgccgcc gctagcgaac
    34921 gcctgagcga cgagctaaaa caggcgatgg cggtggcagt aaaaaatatt gaaaccttcc
    34981 acactgcgca aaaactgccg ccggtagatg tagaaacgca gccaggcgtg cgttgccagc
    35041 aggtcacgcg cccggtcgct tcagttgggt tgtatattcc tggcggctcc gccccgctct
    35101 tctcaacggt attaatgctg gcgactccgg cgcgtattgc gggctgtaaa aaagtggtgc
    35161 tgtgctcacc gccgccgatt gccgatgaga tcctttatgc ggcgcagctg tgcggtgtga
    35221 aggacgtgtt taacgtcggc ggcgcacagg ccattgccgc gctggcgttt ggtacggaat
    35281 ctgtgccgaa agtggacaaa atcttcgggc cgggtaacgc ctttgtcacc gaagcgaaac
    35341 gtcaggtgag ccagcgtctg gacggtgcgg ctatcgatat gcccgcaggc ccgtcggaag
    35401 tgttggtcat tgccgacagc ggcgcaacgc cggatttcgt ggcttctgat ttgctttctc
    35461 aggctgaaca cggcccggac tcacaggtga ttttactgac gcctgacgct gatatggcgc
    35521 atcaagttgc cgaagccgtc gaacgccagt tagcagaact gccgcgtgcc gaaaccgccc
    35581 gccaggcact gaacgccagc cgcctgatcg tgactaaaga tttagcgcag tgcgtagaga
    35641 tctccaacca gtacggcccg gagcacctga tcatccagac ccgcaacgcc cgcgatctgg
    35701 tcgatggcat caccagcgcc ggttcggtat ttcttggtga ctggtcaccg gaatcggcag
    35761 gtgattacgc ctccggcacc aaccacgttc tgccgactta cggttacacc gccacctgtt
    35821 ccagcctcgg gctggcagat ttccagaagc gtatgaccgt gcaggaactg tcgaaagaag
    35881 gtttctccgc gctggcttcg accattgaaa cattggccgc cgccgagcgc ctgaccgccc
    35941 ataaaaatgc cgttactttg cgtgttaacg cccttaagga gcaagcatga gcaccgtgac
    36001 tattaccgat ttagcgcgtg aaaacgtccg caacctgacg ccgtatcagt cggcgcgtcg
    36061 tctgggcggt aatggcgacg tctggctgaa cgccaacgaa taccccactg ccgtggagtt
    36121 tcagcttact cagcaaacgc tcaaccgcta cccggaatgt cagccgaaag cggtgattga
    36181 aaattacgcg caatatgcag gcgtaaaacc ggaacaggtg ctggtcagtc gtggcgcgga
    36241 cgaaggtatt gaactgctga ttcgcgcttt ttgcgaaccg ggtaaagacg ccatcctcta
    36301 ctgcccgcca acgtacggca tgtacagcgt cagcgccgaa acgattggcg tcgagtgccg
    36361 cacagtgccg acgcaggaca actggcaact ggacttacag ggcatttccg acaagctgga
    36421 cggcgtaaaa gtggtctatg tttgcagccc caacaacccg acagggcaac tgatcaatcc
    36481 gcaggatttt cgcactctgc tggagctaac ccgcggaaaa gccattgtgg ttgccgatga
    36541 agcctatatc gagttttgcc cgcaggcatc gctggctggc tggctgacgg aatatccgca
    36601 cctggctatt ttgcgcacac tgtcgaaagc ttttgctctg gcgggccttc gttgcggatt
    36661 tacgctggca aacgaagaag tcatcaacct gctgatgaaa gtgatcgccc cctacccgct
    36721 ctcgacgccg gttgccgaca ttgcggccca ggcgttaagc ccgcagggaa tcgtcgctat
    36781 gcgcgaacga gtgacgcaaa ttattgcaga acgcgaatac ctgattgccg cactgaaaga
    36841 gatcccctgc gtagagcagg ttttcgactc cgcaaccaac tacattctgg cgcgctttaa
    36901 agcctccagc gcagtgttta aatctttgtg ggatcagggc attatcttac gtgatcagaa
    36961 taaacaaccc tctttaagcg gctgcctgcg aattaccgtc ggaacccgtg aagaaagcca
    37021 gcgcgtcatt gacgccttac gtgcggagca agtttgatga gtcagaagta tctttttatc
    37081 gatcgcgatg gaaccctgat tagcgaaccg ccgagtgatt ttcaggtgga ccgttttgac
    37141 aaactcgcct ttgaaccagg cgtgatcccg gaactgctga agctgcaaaa agcgggctac
    37201 aaactggtaa tgatcaccaa tcaggatggt ctgggaacac aaagtttccc gcaggcggat
    37261 ttcgatggcc cgcacaacct gatgatgcag atcttcacct cgcaaggcgt acagtttgat
    37321 gaagtgctga tttgtccgca cctgcccgcc gatgagtgcg actgccgtaa gccgaaagta
    37381 aaactggtgg aacgttatct ggctgagcaa gcgatggatc gcgctaacag ttatgtgatt
    37441 ggcgatcgcg cgaccgacat tcaactggcg gaaaacatgg gcattaatgg tttacgctac
    37501 gaccgcgaaa tcctgaactg gccaatgatt ggcgagcagc tcactaaacg agaccgttac
    37561 gctcacgtag tgcgcaacac caaagagaca cagattgacg ttcaggtgtg gctggatcgc
    37621 gaaggtggca gcaagattaa caccggtgtt ggcttctttg atcacatgct ggatcagatc
    37681 gccacccacg gtggtttccg tatggaaatc aacgtcaaag gcgacctcta tatcgacgat
    37741 caccacaccg tcgaagatac cggcctggcg ctgggcgaag cgttaaaaat ggctcttggc
    37801 gacaaacgcg gtatttgccg ctttggtttt gtgctaccga tggacgaatg ccttgcccgc
    37861 tgcgcgctgg atatctctgg tcgcccgcac ctggaataca aagccgagtt tacctaccag
    37921 cgcgtgggcg atctcagcac cgagatgatc gagcacttct tccgttcgct ctcatacacc
    37981 atgggcgtga cgctacacct gaaaaccaaa ggtaaaaacg atcaccaccg tgtagagagc
    38041 ctgttcaaag cctttggtcg caccctgcgc caagccatcc gcgtggaagg cgacaccctg
    38101 ccctcgtcga aaggagtgct gtaatgaacg tggtgatcct tgataccggc tgcgccaatc
    38161 tgaactcggt gaagtcagcc attgcgcgcc acggttatga accaaaagtc agccgtgacc
    38221 cggacgtggt gttgctggcc gataaactat ttttacccgg cgttggcact gcgcaagcag
    38281 cgatggatca ggtgcgtgag cgcgagctgt ttgatctcat caaagcctgt acccaaccgg
    38341 tactgggcat ctgcttaggg atgcaactgc tgggtcggcg cagcgaagag agcaacggcg
    38401 tcgacttgct gggcatcatt gacgaagacg tgccgaaaat gaccgacttt ggtctgccgc
    38461 tgccgcatat gggctggaat cgcgtttacc cgcaggcagg caaccgcctg tttcagggga
    38521 ttgaagacgg cgcgtacttt tactttgttc acagttacgc aatgccggtc aatccgtgga
    38581 ccattgccca atgtaattac ggcgaaccgt tcaccgcggc ggtacaaaaa gataacttct
    38641 acggcgtgca gttccacccg gagcgttctg gtgccgctgg cgctaagttg ctgaaaaact
    38701 tcctggagat gtgatgatta ttccggcatt agatttaatc gacggcaccg tggtgcgtct
    38761 ccatcagggc gattacggca aacaacgcga ttacggtaac gacccgctgc cgcgcttaca
    38821 ggattacgcc gcgcagggtg ccgaagtgct gcatctggtg gatctgaccg gggcaaaaga
    38881 tccggcaaaa cgtcaaatcc cgctgattaa aaccctggtc gcgggcgtta acgttccggt
    38941 gcaggttggc ggcggtgtgc gtagcgaaga agacgtggcg gcgttactgg aagcgggcgt
    39001 tgcgcgcgtg gtggttggct ccaccgcggt gaaatcacca gaaagggtga aaggctggtt
    39061 tgaacgtttc ggtgccgatg ccttagtgct ggcgctggat gtccgtattg acgagcaagg
    39121 caacaagcag gtggcggtca gcggctggca agagaactcg ggcgtttcac tggaacaact
    39181 ggtggaaacc tatctgcctg tcggcctgaa acatgtgctg tgtaccgata tctcgcgcga
    39241 cggcacgctg gcaggctcta acgtctcttt atatgaagaa gtgtgcgcca gatacccgca
    39301 ggtggcattt cagtcctccg gcggtattgg cgacattaac gatgttgctg ccctgcgagg
    39361 cactggcgtg cgcggcgtaa tagttggtcg tgcattactg gaaggtaaat tcaccgtgaa
    39421 ggaggccatc gcatgctggc aaaacgcata atcccatgtc tcgacgttcg tgatggtcag
    39481 gtggtgaaag gcgtacagtt tcgcaaccat gaaattattg gcgatattgt accgctggca
    39541 aaacgctacg ccgaagaagg tgcagacgaa ctggtgttct acgatatcac cgcttccagc
    39601 gatggccgtg tggtagataa aagctgggta tctcgcgtgg cggaagtgat cgacattccg
    39661 ttctgcgtgg cgggtgggat taagtctctg gaagatgccg cgaaaattct ttcctttggc
    39721 gcggataaaa tttccatcaa ctcccctgcg ctggcagacc caaccttaat tacccgcctg
    39781 gcggatcgct ttggcgtgca atgcattgtg gtcggcattg atacctggta cgacggcgaa
    39841 accggtaaat atcatgtgaa tcaatatacc ggcgatgaaa gccgcacccg cgtcactcag
    39901 tgggaaacac tcgactgggt acaggaagtg caaaaacgtg gagccggaga aatcgtcctc
    39961 aacatgatga atcaggacgg cgtgcgtaac ggttacgatc tcgaacaact gaaaaaagtg
    40021 cgtgaagttt gccacgtccc gctaattgcc tccggtggcg cgggcaccat ggaacacttc
    40081 ctcgaagcct tccgcgatgc cgacgttgac ggcgcgctgg ctgcttccgt attccacaaa
    40141 caaataatca atattggtga attaaaagcg tacctggcaa cacagggcgt ggagatcagg
    40201 atatgttaac agaacaacaa cgccgcgaac tggactggga aaaaaccgac ggactaatgc
    40261 cggtaattgt gcaacacgcg gtatccggcg aagtgttaat gctgggctat atgaacccgg
    40321 aagccttaga caaaaccatc gaaagcggca aagtcacctt cttctcgcgc actaaacagc
    40381 gactgtggac caaaggcgaa acgtcgggca atttcctcaa cgtagtaaat attgcgccgg
    40441 actgcgacaa cgacacgcta ctggtactgg cgaatcctat cggaccaacc tgccacaaag
    40501 gcaccagcag ctgcttcggc gacaccgctc accaatggct gttcctgtat caactggaac
    40561 aactgctcgc cgaacgcaaa tctgccgacc cggaaacctc ctataccgcc aaactgtatg
    40621 ccagcggcac caaacgcatt gcgcagaaag tgggcgaaga aggcgtagaa accgcactgg
    40681 cagcgacggt acatgaccgc tttgagctga ccaacgaggc atcggacttg atgtatcacc
    40741 tgctggtgct gttgcaggat caggatctgg atttaacgac ggtaattgag aacctgcgta
    40801 aacggcatca gtgagttgca gggtaagcgg atgcgatgtt gttgtcgcat ccggcaaaaa
    40861 aaaagggcaa ggtgtcacca ccctgcccgt tttctttaaa gctgcaaaga ttacttcgcg
    40921 ttataattac gcagagcgtt acgccccagc acaatccccg cgccaaccat gccgcccagc
    40981 agcaccgcca gaatcaaggt aattgccttt ttcgggctat cgcgacgaat aggtaacgtc
    41041 ggtttcatca cataacggta agcatgaata tcaagatcat caacctttaa gttttcaata
    41101 tccaacaggt tttgacgtgt ctgatagtag tttggtgaga acaccaacgg acgggtcgcc
    41161 tcatgcttaa tcatcgactc cagcgcttcg ctccccaaaa ggaacaacgt atcttgtgtc
    41221 acatcttcag tctgctgaac ctgtggcttt gccacctgcg cctgattcgc atactgcaac
    41281 gcttcctgaa tctgacggat acgcagatct ttctgctcct gcgcgactac ttcctgcgtt
    41341 cttaaagagt cctgcaagtt tttccgtccc agagcaatgt tgtctttgag atccttttct
    41401 agctcttgat tcactttatc atcaacttgc tgaatgtatt gggccaactt catttgtgct
    41461 ccctctgcag tttgcccaac ataagaaaca gtcaatggta attgctggtt cttaacagaa
    41521 ggttcgatgg taagtttttc tggttcttcc tgattatcca gcgtttctgc taatgcagag
    41581 aaggcagaac tgaagcgacc aattaacgtc tcctgtaaat ccgatacttt cggtgcagcc
    41641 tgaccataga taacattcat ggcattgtta tagccagcaa tttgccccac gtcgggctga
    41701 gtgataattg ctgttgacgt ccatttctcc ttcgctactg ccaaatatcc aatagccagg
    41761 gcaatagcca caatgacgga aatgatgatt gtcatcttgc cacgccacaa ctgcactagt
    41821 aaatcaatca aatcaatctg ttccgggtca tggttttgcc cagaaacatt attattttct
    41881 actctcatca ttaccctaac tgacggaaat ttccaagtcc gcatagttta gcgataatcc
    41941 tagtgaatgc tataggataa gtgataaaaa taatgaatta ttggggaggt aacaaaagac
    42001 aatcagggcg taaatagccc tgataacaag atgttagtcg ctgccaaaga gatcgcgggt
    42061 gtatacctta tctgccacat ctttaagctc ttctgccatt cggttagaga taatgacgtc
    42121 ggcttgttgt ttgaagatgg cgagatcacg ttccagccga gagttgaaga atgagtcttc
    42181 tttcatcact ggctcgtaga tgatcacttc aacgcctttc gccttgatac gcttcataat
    42241 cccctgaata gaagacgcac ggaagttatc tgaaccgctc ttcataatca gacgataaat
    42301 acccaccact tgcggtttgc gtgacaaaat ggcatcggca ataaaatcct tacgcgtgcg
    42361 gtttgcatcg acaattgccg agatcaggtt attcggcaca gactgatagt tcgccagtaa
    42421 ctgcttggta tctttcggca agcaataacc accataacca aacgacgggt tgttgtaatg
    42481 gttgccaata cgcggatcga gacaaacgcc ttcgataatc tggcgagtat tcagacctaa
    42541 actttctgcg tagctatcca gttcgttaaa gtatgccacg cgcatcgcta ggtaggtgtt
    42601 tgcaaaaagt ttaatcgctt ctgcttcggt cgaatcggtg aacagggtcg ggatattttg
    42661 cttaatcgcg ccttcctgta acaacgcagc aaaacgttct gcgcgttctg aacgctcacc
    42721 gatgacaata cgtgaaggat ggagattatc gtaaagggct ttaccttcac ggagaaattc
    42781 cggagagaaa ataatatttt cagtacgata tttcttatgc atcgcttcgg taaaaccaac
    42841 gggaaccgtt gatttgatga ccataaccgc ataaggattt atctcaacta cgtctttaat
    42901 tactgattct acactggatg tattgaaata attagtttta ggatcatagt cggttggagt
    42961 ggcgatgatg acataatcag catcccggta agcttcattt ttatctaatg tggcattaaa
    43021 gtgtatttta tctgattgca aaaactgctg aatttcctta tcaacaatag gagatatccg
    43081 atcattcagc atagcaacgc gtgacggtaa aatatctaat gccacaacct catgattttg
    43141 tgcgattaga agcccgtttg acaagcctac atagccagta ccggaaatgg tgattttcat
    43201 ttcatgctct cagaattaac ttaactgtga atcatgatgt ttttagcatc ctgataagag
    43261 ctaaatgttt taacgctacg ttattagctg gtcaacgcta cgaaaaacaa ttcagatttt
    43321 ctttataaga atagctgata tttattaagt taatattaag cagtgaaatt tagtctataa
    43381 gataaatggc aaaaaaaaag cccggtgcaa tatacgccgg gcctcaattt tactgttggt
    43441 taaatcagat taatccagcc attcagtatg gaacacacct tctttatcaa tgcgcttata
    43501 agtatgtgca ccgaaatagt cacgctgtgc ctggatcagg ttcgcaggca gaacagcggc
    43561 acggtagcta tcgtaatagg caaccgcagc ggcgaaggtc ggaaccggga taccgttctg
    43621 tactgcataa gcaacgacat cacgcagcgc ctgctggtag tcatcggcaa tttgcttgaa
    43681 gtacggagcc agcagcaggt tagcgatctg cggattttcg gcatatgcat cggtgatttt
    43741 ctgcaggaac tgcgcacgga tgatgcagcc agcacggaaa atcttcgcga tttcgccgta
    43801 gttcagatcc cagttgtatt cttcagacgc agcacgcagc tgagagaagc cctgagcgta
    43861 agaaacgatt ttgcccagat acagcgcacg gcgaactttt tcgatgaact cagccttgtc
    43921 gcctgctgac tgagcttgcg gaccagagag aactttagat gcggcaacgc gctgatcttt
    43981 cagagaagag atataacgtg caaacacaga ctcggtaatc agcgacagcg gttctccgag
    44041 atccagcgcg ctctggctgg tccatttacc ggtacctttg ttagccgctt catccaggat
    44101 cacatcaacc aggtagttac cgtcttcatc ttttttagtg aagatatctt tggtgatgtc
    44161 gatcaggtag ctgctcagtt caccgttatt ccactcggta aaggtctgtg ccagttcttc
    44221 gttagagaga ttcaggccgc ctttcaggag agaataggct tcagcaatca gttgcatatc
    44281 accgtattca ataccgttgt gaaccatctt cacataatgg cctgcgccat cagcaccgat
    44341 ataggtaacg cacggttcgc catcttcagc aacggcggcg attttggtca ggatcggcgc
    44401 aaccagttca taggcttctt tctggccacc aggcatgatg gacggccctt tcagcgcccc
    44461 ctcttctccg ccggaaacac cggtaccgat gaagttaaag ccttcggcag aaagctcacg
    44521 gttacgacga atggtgtcct ggaagaaggt gttaccacca tcgatgatga tgtcgccttt
    44581 atcgagatat ggtttgagcg aatcaatagc agcatccgtg cctgcacctg ctttcaccat
    44641 taacaggatg cgacgaggcg tttccagaga ttcaacaaac tctttcaccg tatagtaagg
    44701 aaccagtttc ttgcctggat tttcggcaat cacttcttcc gtcttttcac gggaacggtt
    44761 gaaaatagag acggtataac cacggctttc gatgttgagc gcaaggttgc gccccatcac
    44821 tgccataccg actacgccga tctgttgctt tgacattgtt tactcctgtc agggggtggt
    44881 caccgccgag tgtgcggctt aaatgtggta tagatgttaa ctcactcata gagatggtga
    44941 atagcattac ctttgctata ccacactgaa tatctcacct tttccgtctt ttaaaactta
    45001 tttatatgaa attactgaat tacttgttca gtaactcaag aataagtttt gtcttttctt
    45061 ccatcagcgg cacatcaccg cgagattcca cattcaaccg caccaccggt tcggtgttgg
    45121 aggtgcgcag gttaaagcgc cagtcggcaa aggtcatgct gatgccatcg gtgcgatcca
    45181 ccgccagcgc ctcgcggcta aaatgctgtt ccacgcggtt aatcgcctca aagggttgcg
    45241 ccagtttgct gttgatctca ccgcttgccg gaaacgctgc catccggtcg cgcaccagtt
    45301 cgcccagcgt ttttcctttc aggcacacca gttcggcgac cagcagccac gggatcatgc
    45361 cgctgtcgca gtaagcgaaa tcacggaagt aatggtgggc gctcatttca ccaccgtaga
    45421 tggcgtcttc cttgcgcatc cgttctttaa taaaggcgtg tccggttttc gacatcaccg
    45481 gcgtgccgcc tgcggcagtc accacatcaa cggtgttcca ggagagacgc ggatcgtgga
    45541 tgatcttcgc gccgggattt ttttcgagga atgcttctgc cagcaggccg acaatgtagt
    45601 agccctcaat aaactgccct ttttcgtcaa acaggaagca gcggtcaaaa tcgccatcaa
    45661 aagcaatgcc catatccgca ccgtgtttga tgaccgcatt gcgggtgtcg tcgcggcatt
    45721 ccggcagtag cgggttagga ataccgttgg ggaaattgcc gtccggcgtg ttgtgcactt
    45781 tgattaattc cacgggtgcg ccaagggctt taaagcgggc ttcgatggcg tccaccaccg
    45841 gacccgctgc gccattcccg gagttaatca ccagcttgag cggcgtgagg tttttgacat
    45901 tgatataacc gaacaggtga tcaacgtaag cgtcacgcag attgatttgc tgatagcgac
    45961 cgcgtttggt ttcatcaacg ggaggaaagt cgttggcttc tgccagacgc tggacatcgc
    46021 gcagtccggt atcaccgctg atcgggcgag ccccttcgcg caccagtttc atgccgttgt
    46081 aatccatcgg gttatggctg gcggtaactt caatgccgcc atccacgccg agatggaacg
    46141 tggcgaaata gatctcttcg gtgccggaca tgccaatatc cagcacatcg acgcccgcat
    46201 cctgtaaacc tctcgccagc gccagtttta aggtttcgct ggtgaggcgg acatcaccgc
    46261 ctaacacaat ggttttcggt ttgagaaatt cgccataggc gcgaccaatg cgccaggcga
    46321 tatcttcatt cagttcttcg cctaatttcc cgcgaatatc ataggcttta aagcaggtta
    46381 attttttcat gtcgttaccc tttttcaggc aatagttggc cctgaccgaa acggccaatt
    46441 ttgtttttgt cgttatttac atctcggttt cgcattcatt gcctgatgcg acgtttacac
    46501 ccgtccgtag cgatccgcga aacgcaccac atcatcctct tcgagataag agccggagcg
    46561 cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcaatgca tcgcccccag
    46621 cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa tggtgacttt
    46681 tgccgttccc gcgacaacca cccagtgttc cgcgcggtga tggtgcatct gtaccgacaa
    46741 gccctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg cgtcgataga
    46801 gtcatatttg ccccacgggc gatagacttc gcgatgcacc cgatgctcat ggcgaccatc
    46861 ggctttgatc tgctcgacca cttttttcac atcctgcacc gcgttacggt cggcaatcag
    46921 caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg ttacgaggcc
    46981 agattcggcg tacacatagc tgttttcagt tttgtgatta atcacatcgc cgtggcaaac
    47041 gttgccctcg gcggtgtggg cgctaatttc ccataatgag gaccaggagc cgacatcgct
    47101 ccagcccgca tccatcggca ccacaacggc atctgccgta cgttccatga ccgcgtaatc
    47161 caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa aatcgagatc
    47221 cggatcgacg gcgctcatcg ctttttcaca ggcatcgaga atatccggac gatatttttt
    47281 cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc agtaatattc
    47341 gccgcttgcc acataggcct gggcggtttc cagattcggt ttttcgacaa actgcgccac
    47401 ttcaaaggcc accgtatcct gctcacccgc agacacttca ccgcgacgaa tatagccata
    47461 accggtttct ggtagatccg gcacaatgcc gaaggtcacc agcttgcccg cttcggcata
    47521 tggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca catgatccgc
    47581 cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg ccagcgccgc
    47641 cagcgcaatg gcaggtgccg tgtttcgccc tgccggttcg agaataatgt tctcggtaag
    47701 tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat tgcaaatcac
    47761 caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca gcatggtgag
    47821 atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg gccataagcg
    47881 gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca taattatccc
    47941 cgaatatcat ttataaattg acgtaacacg ttctctttat cgagtgtgcg ttcggcatat
    48001 tcacgtgcca ccgtgttgtg tttgggcagc aggagcgcct gacggatccc cgccaccagc
    48061 gcctcgaccg attccggttc tacgcaaacc gcaatgcccg gaaaggtttc gcaaagctgc
    48121 cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc cagaatattg
    48181 gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac cagatggcaa
    48241 tcgcccatct tcagcagtgc gggtaaagcg tcatacgatt gcagcgggaa aaattgcatg
    48301 ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc gccctgcccg
    48361 acaatggcaa aaatcagcgg ttcatcgcgc agacgatcgg cagcttcaat aacgttttcc
    48421 agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt atccggcagg
    48481 tcaagctggt tacgaagggc gtcaacatcg gcatctgcaa catgctgaaa acgggcaatt
    48541 tccgaccagt tggggaagaa gatgacgttt tccgccgcca cgcctttttc gatggcttta
    48601 ttcatcatcg aacgcgaaat cgtggagacg ttatcgacgt tatgcagtcc gctacgttcg
    48661 aacgccgttg ccagctgtgc cactttgctg cctttgcctt ttccggcaag gcccagcccc
    48721 agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc agaaagtttc
    48781 gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ccacgccgat aatgcgatcc
    48841 ggcttccagc gacgttgcgc catcagagga aagaaactgc tgacggcaaa actgccgaga
    48901 tgtaacaggc gtttcagggt gctcggctgt tttggcacgt acagcgggca gcgccacacc
    48961 gtggcggccc cctcttctcg tttgtaccgc caggcggaat agttctcgcc cacctgccac
    49021 tgcgggtagt aaggcggtgc ggtaataacc cgcacctcat gaccttgtgc cgccagccat
    49081 tccaccatct cgccggtgta tttgccgata ccggttaact ccggcgagta gttaatgccg
    49141 tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga aaataggcgc
    49201 ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc cagcggtaat
    49261 cgtcatgctg ctcatccggc agtatcagct cttcttcagc gactctgaag cgaaaaccga
    49321 gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa aagtgctgcc
    49381 agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc gccatcgtca
    49441 gccgctcgaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc ggcacaaacc
    49501 agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcaccg cgactgttct
    49561 cgacaataaa gtcgagagag acaagcgggg tggagcgcac taccgtggca aagtcttcct
    49621 gacgtaaaaa catcattacc cccgaaagcg gtcttgattc tcaaggaacc actggtaagt
    49681 gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat gcaggcgcgt
    49741 cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa ccacccgacc
    49801 tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc agtcaacgcc
    49861 cgtgccgacg ttaatgtgcg acagcatcgg ctgggtgttc tccagccaga cttcgtgcgc
    49921 cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga attcacgcat
    49981 cggtgtaccg ctgccccata ccaccacgtc cggtgcattc tgtgccgtcg cctcgtggaa
    50041 gcgacgcagc aatgcaggga tcacatgcga attactcggg tggaagttgt catgcgggcc
    50101 atacaggttg gtcggcatga ctgagcggta atcgcgtccg tactggcggt tgtatgattc
    50161 gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg gctccagcgt
    50221 gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt agatacagga
    50281 cgatccaaga aacagcagtt tgttcacgtc gttctgatgc gcggcgtgaa tgatgttgct
    50341 ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg caacaatgcc
    50401 gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa agaaatcatg
    50461 caccgcgcgg ctgtccagca ggttcagctc gtcgcgggtg cgtaatacca gttccacatc
    50521 accgcgctgt tcgagctgcc gcctgatggc agaaccgacc atcccacgat ggccagcgat
    50581 aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc gtagccgtga
    50641 gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat ttcagacacc
    50701 atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt ggtcgggtcg
    50761 ccgagcagcg tttcaacttc agccggacgg aagtaacgcg gatcaacggc gataatcaca
    50821 tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat gcccttctct
    50881 tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgccgctgc catttccacg
    50941 aactgacgca cggagtactg aacgccggtc gcgataacga aatcttccgg ctgttcctgt
    51001 tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc acgcagggaa
    51061 tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc gattgcgcgg
    51121 gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt gaagagaatt
    51181 ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca gtaggcgtac
    51241 agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt ctgcggaatt
    51301 tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt tttcttttcc
    51361 agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc gacgtcagcg
    51421 gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcccag gttgtacact
    51481 tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact caggtcgcca
    51541 taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg atccacgcgc
    51601 tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc tttttccagc
    51661 agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag agcgactttt
    51721 gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt cgcgtatcac
    51781 cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa cactgcttcg
    51841 tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga catcggttgc
    51901 cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat gttgacttgc
    51961 atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat gtgcgccaat
    52021 ggttatttca ccgagggtat ataaattgac gtcatcgccg acccacgcgt aatcacctaa
    52081 ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt tttttcctat
    52141 ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt gtggcgacca
    52201 ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt tgccgccccg
    52261 gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt atattcagac
    52321 tttgttatat aaggctttcg tctttgaggt agtacgttgg cgtaaatgcc aggataattc
    52381 agcccaaaag ccaggcacat gtaatatttg tcgttggact tttttcgcgt cagcacacaa
    52441 ttccatatta ttggtggtag ataccccacc catggaaaat tcagacacca ggccattgag
    52501 ttttttaaat gcataaccgg ctttatacat tttggctgcc agcgcgtagt cggaagaaac
    52561 tttatattcc aggtcataac gccatttttt caagccggat acagggaaaa atatcgcctg
    52621 atgactggcg ggcaggctgt gataaatata ccagcccggt ttggcgctac gtttaatttt
    52681 atgcccgtcg ccaaaatcca gcagcgcatc gccggtgatc atcatgttat ctttttgcat
    52741 ttttaactta cggacaaaat ttgcggcatc ctgatgaaaa atatcgcccg aattgagaaa
    52801 caacgcgaac ttgccttgtg ccatcgtaat gcctttgttc atggcgtcgt agataccgtt
    52861 atctggctcg ctgacaaagc gtaggttata gataccattg agattttcca gatactcacg
    52921 ggtgccgtca ttggaaccgc catcgacaac aatccattcg aagctgatat cttccgcctg
    52981 cgccagatgc gccagcgagg catgtgtttt gactatccct tcgaggttac gaaacgcgac
    53041 agtgattatg ctaagcaaca tgttcttatt cttacctcgt aatatttaac gcttttcgca
    53101 aaataaacgg acagacgatt aaaaatgcat attccgggct aaatatcgaa ccggtaaaaa
    53161 acagcgatac cggagtaaaa agatataatt gcacgcgaaa attgcggtta tcaccaaaag
    53221 cgttgatcat catttttatc actttcccca tgtaccacag cgataaaaac actgcgaacc
    53281 aggaaaaata aataatcagc agatacaaac cattgtctat ggtttttccg acatccgcac
    53341 cgttaaatat tccgaatgat gcgacgtatt cataaagtga gccaaatctg actacaccgt
    53401 caatatgggt caaagaataa ccgaccatca ccagcggacc gacaatacga taatacgatg
    53461 acgatccttc cgtacctaaa tcgcccagac gggtggaaat ataaggaaac gcgattacca
    53521 caccaacccg gaatacagcc agagaaatca atgccaacgg taactttttc ttaatcgcct
    53581 ctttattcag atattgaaat gcccactcca gcaaataaaa caggataaaa gtcataaccc
    53641 ctgaaaacga tccggataat attatccctg cgagaatcat agcatcggtt ttaggcgttt
    53701 tgataccaaa ctgtttgatg ctgagccaaa ttgagattaa tgccagagcg aaaaatgccg
    53761 gttcgaaata cagtgctgtc gtgcgcttgc cgccgaattt aatgaaattc agtacatagc
    53821 tgttgctgta aatcagatat ttcgaaatcg actccataat actgctgcca ccggtgagga
    53881 taatttgcgc catctccacc gccgccagcg ccaccaccag cccgatcacc agataaaaga
    53941 aacgtaatat cttgcgatgg ttgtgtggcg aaatcgtttt aaagcgaata ctccacacca
    54001 tgccaataat gatcacaata tagacaaaca gcatggttga agtgacgtat ttactggcat
    54061 ccagcgactg accaaacaga tagttaaacg ccgtgagtcc cgcaccaatc cctaaggcaa
    54121 tcatcaattt tttaacgctg atgcgttcta aaaacagcag taacaagaca ggtaaaaaag
    54181 tgacgatggt gatggggaaa ctttcgccaa gctgggcgat tttgacgtta accagcaggt
    54241 agatcagcgg cagcagcagg tagctacaga ttctgataga agttgacata ctcctccagc
    54301 atctgttgtc cactgtaggt ggcgcggctg cgttggctga actcagccag cgtggtacca
    54361 aatatcgcct gcgcgatttc cggtttgctt aactgcacca gttgcagcac gtcttcttcg
    54421 tcgacggttt taccgccgga tttttgcaac acttcccgtg ccgcgtcgct atgggtggca
    54481 atcaccggca cgccaattga taacgcctca cacaaaatca gcgggtagtt atcgacgcga
    54541 gaactgaata ccagcgcatc catctgattg agcgcgctca tcaacttgcg cttgtcagtt
    54601 tcaaagccgt gattaaccac gttgccagcg gtgaacggcg agaacttacc aaaggtatgc
    54661 agttcgattt tgtcgcccag cgccatcatc tcacgcacca gttgctggtt agtcttgccg
    54721 tcgtaacgca agtcatgcgc caccaccgcg attttcggct tgccctgggt ttcgcgcacc
    54781 ggaggcaagt ccgccagaat cgcttcggtt gccatatcaa taccattatt gataatccgg
    54841 caacgccctg gaccgtacag gctattgaaa gcgtcagcca catgctggct gggggaaata
    54901 aactgacagc ccagcgccag catctcacgg aataactggc gtttgcccgc caccagctgg
    54961 tgtgcgcgat caatcttcac cggcggatag ttatttaagg tcgggcattt ctggcagccc
    55021 gttttccagc cttcgcaacc gtcggtaaag gcgcagcgtc cggtaacact ccagtggtcg
    55081 tgcagcgtcc agaccagagt gacgtccggt ttatggtttt tcactttttc gcaaaagcgc
    55141 accacgctct taagatttag ccagtagctg tgcagcacat gaaaatgcag gaccaccgga
    55201 cccggtgtgc gagtaatggt gcgatataac tcattgaaat tgccaaacag atcgcgatta
    55261 aacagacgaa acagggcaat attcgccatc gcggtcatcc gcggcgtatg tttgatgacc
    55321 tgcggatagt tctgatggct gacgctctct ttgccgcctt tgccataacc gtagacaaaa
    55381 tgtgacgcca gcccctgttg cagcgcacgc tggtggagat ctaacgccac acctgctgcc
    55441 ccgccttccg ccagtcgcac attaaattgc agaatattca tttaattacc ttcactcgcg
    55501 ctttttctcc caccaccagc gcgttgtccg ggacagagtc gagcaccacg ctgcccgcac
    55561 ccacggtgac gttgttacca agcgtgatat caccaagaat aatgacgttg gcaccgagtt
    55621 cgacgccgtt gccaatgtgt ggacatgcca tgttatcggc accacgattg ccgatagtga
    55681 cgccgtggcg aatggtgaaa tcatcccccg ctaccacgtt tttattgatc actacggcgt
    55741 aaccgtgatg gatagtaaag cggcggccaa tggtcgcggc ggcctggatt tcataaccga
    55801 aaaagcattc ggtgataatg cgatacagca ccagcagcgg ggccgcccac agattgttga
    55861 ggacgttctt tttgcgccac accgaacaaa aatgagcaac acgataggca agaaccatgc
    55921 agcacgggcg taaactccag ctgttggcac gcagatcttc cagcatcttt agcgcccccg
    55981 aataccatca gccagacgtt tgccgttacg caccgacagc agcgtcagca aagtgcgcca
    56041 ggtcatacgt ttattgcgga tctggtagag ggtaaacagc tgatattttt tgcttgcacg
    56101 gtcaaattta tctttgtgct ttcggtaaaa gtggaagtac ccggagaatt ttttcggtga
    56161 cgaggtgatc tgcatttcac cgtgattgat atgcaggatc tgcgtcgctt cttccacttt
    56221 ccacggttcg ccgtactcca ccaccatccg caggaagatg tcgtaatcct gtgctgcttt
    56281 cagttcggta tcgaacaaac actctttgaa acgccatgcc caagtaaaga cctgattgcc
    56341 aatgatattg cgtttataga acaggcggcg tgagtacggc gatttgggat acagtggtaa
    56401 gcttgccggt tgggaataaa cttctccctg gcatacgtag tcgttagcgt acaaaaaggc
    56461 gtgagtgacc agttgctgtt tatgggcgag gaagacgctc agacggttgg gtgtccattc
    56521 gtcatcgtca tcgatcccgg taatgtattc cccttgcgcc agcataatcg cctggttacg
    56581 taccgcgcac gccccgctgt taatgtcgtt gtgaatgtag gtgatacgcg gatcgttgag
    56641 ggcagtgacg tactgttgca gttgttccca ggaggtggag caatcatcca cgatgatcat
    56701 ctcccagttg ctgtagtcct ggcgcaggac cgattttatc gcccgaatcg ccagttgttg
    56761 acggttccag gtcggcatat agattgaaat cagcggattc tctttcatag gttgttctcc
    56821 cggcttatga gctggtttgc ttgccggatg cggcgtgagc gccttatccg gcctacgggg
    56881 cggtgcgaat gcaggcgtcg ttatttcgca tccgacttat attcgtattc gtaatagcca
    56941 taatcctgat acgcgctggc gcggcggaag atggagttca gaatcacccc tttcaccgga
    57001 ataccgtttt gctcaaagcg gctcagactg gtttccactt ctttcaatgt gttgaccgca
    57061 tagcgcgcca ccattaacgt ggttccagcg tgacgcccga caattgctgc atcggtcact
    57121 gccagaatcg gcggcgtatc aatcaacacc aggtcgtagt ttttactcgc ccagctcacc
    57181 aattcggcaa agcgttcgct catcaacagt tcagaaggat ttggcggcac ctgaccgcgc
    57241 gggatcaggt caaatttggc aatagaggtc ggtttagctg cggtagtaat atcgccctgg
    57301 ccaatcagaa tttccgacag gccattgacg ttattggtac ccaacagctc gtgggtgtag
    57361 cctttgcgca tatcgcagtc gatcaacaac acccgtttat tggtctgact gatcaccgcc
    57421 gccaggttgg cgcagacaaa ggttttaccg attgacgggc tcacccctgt catcatcaac
    57481 acattgttct gcgcctgcat catcgcgaag tgcagactgg tacgcagact gcggatggct
    57541 tcaatcgcca gatcggttgg attccccacc gccagtagct ggctctgttt atagcgttta
    57601 atccctttga tggttttgac gctatcgcgc gctttctgcc attccgacag cgggatgctg
    57661 gcatagacgc tgataccatg ttcttccagc acctgcgggc tttcgatgcc gcgattaaac
    57721 aacgagcgca gcagcacacc cacgatagag agcatcaggc cgaggataat tgcgccgagg
    57781 ataatcagtc ctttcttcgg tttcagcacg ccaggctggg tgattgccgg gtcaacaatg
    57841 cgcacatcgc cgacggtgct ggcttcggtg attttcagct cctgctcttt attcagcagt
    57901 tgcatataga cctgctgacc agactcgaca tcgcgggtca ggcgcacaat ctcctgctgg
    57961 gttttcggca tcgccgttac gcgaccgtta agtttggctt tctcttcttc cagcgcctga
    58021 cgtttctcca gcagcgtgcg gtacgccggg tgaactttgg tgtacagctt ggagatttcc
    58081 gcctctttaa aggtcagttc gttcaactgc gcgtcgatgt tcaccatcga atcgagcacc
    58141 gctttcgctt ccagcggcag atcaacagaa tctttatcct gacggaaggc attcagtttg
    58201 ttttcggcaa catcgaggcg gctacgtact tccggtaact gttgcgcgag gaaagcgagg
    58261 cttttcgacg cttccgccga tttgcgctca atattttgtt cctgatagtt acgggcgatg
    58321 ctgttaagaa tgtcgcggat ctgttcgcga tcttcaccgg tataagtcag gctcagtacg
    58381 cctgcgtctt tgccgttctc cgttaccgtc aggctgtttt gcagttggtt gatcatcccc
    58441 aacgtggagt atttggtgac ggtaaactca ctgcccgggc gggcgtgaat ggcttcaacc
    58501 atcagcgtga cgccttcttt tttcagcatc tggcccgctt gcccacgggc gctaaagccg
    58561 ccatcactgc tcagggtgta gtttttattg tccagcacat taagcgtaaa cacctggccc
    58621 gccatctctt tcgggcggtt aaaggtagtc actttcaccg tctcgttctg gcgtcccatc
    58681 agtcgctccc agcccgcccc gaaaatcggg aacgtgtttt tgctcactgc aatatcgagg
    58741 tcgagatcgt ccaccgtttt accaagcacc agacgcgaac gaatcaactg gatctcggcg
    58801 tccgatgcag gcggtttgtt ggctaatgcc gaaccaatgt cctgcactaa cgaattgccg
    58861 ctgttttgct cgatttgtac cagtgcgtcg gcactataaa tcggcgttgc gaagaaggtg
    58921 taaaccacgg cacagagggc aaatacagcg gtgatgccaa tcacccacca gcgcgcttca
    58981 atgacggtgc cgaccaggcg accaatatcg atttcatcac tgcccgttac cggagcggca
    59041 tgttgtttta ctttttctgt cattcttata cctgctctgc gttcaatgcc tgcgcccact
    59101 ggcgggcaga ccgttcaagt aatgtgtaca ccgctgcaaa cgtttcccgg cttttgcgat
    59161 acggatcggg gatttcacat tcgttatccc agtgaccaaa cagcatcact ttgccgcgca
    59221 tctcaggtgc catctcgcat aagcgttcga tatggcgctt ttccatggtc aaaatcaggt
    59281 catagttgcg acacaggcgg cggctgattt gtcgtgcaca gtggccttcc agagacagtt
    59341 gatgttctgc ggcgacgctg atagcggtag gatcagcgcc cttaccgacc agcgcgccga
    59401 gtccagcgga ctccactttc agttccgggt gataacgttg cagtaagcgt tccgccgtcg
    59461 gggaacggca aatattgccg acacagacaa ctaatatgtt gttaaacatg acgattacca
    59521 gttatgaatg tcgctggctg tatccgtcat gtaacggaca ccgctaatag ttggcaacaa
    59581 ctgattgatc agacggttcc agcgggaaac cggggcggtg gtgacataca ccacgtcata
    59641 aggttgcagg cggaattctg tcgccatcac cagcgacgtg gcatcggaca tatccagctg
    59701 gtagatattg gcaatcttgc cattacgccc gccctcgcct ttcagcggac gaatgacaaa
    59761 gatgccgctg gcgttggagg tggtcatgtc gataccttca gcattaccca gggcttcagt
    59821 cagggtcatg ccgctaaagt ccattttcag ggtgctctgt ttcttcactt cacccatcac
    59881 aaatactttc agatcatcat tacgcggcac gtagagaata tcgccgggat aaagcagtcg
    59941 gttctgattg aggtcgccgt tttgcatcag cgcctgcaaa gaaatgcgtt cttcacgacc
    60001 attgtgtgtt agcactacgt tgcgccagtc agcggtgtcg gtcaggccac ctgcggcgtt
    60061 gatggcgtcg agaatagtca gtggcacgtt ggtgatcgcc tgttgaccgg atttattcac
    60121 ctgaccggag atataggcct tttgtgagcg gaaggcggcg atattaacgt ccacctgcgg
    60181 gtcagcgatg tacgtcgcta agcgcccggt aatatcactg cggatttcag cgagcgtttt
    60241 cccgactacg tgaaccttgc cgatatacgg gtaaaacata gtgccgtcag gctgtaccca
    60301 gttgccggtg tcgctggagc tgcggtactg accggctggc gtggtgagtt ccgggtgatc
    60361 ccagacggtg acattcagaa cgtcgcccgg cccgacgcga tactggtaat tcgcgatctc
    60421 gctttccagc gtcatattgg ggcgcgctac attcgggcgt gggcgtaatt ggtcaatcag
    60481 gcgcggggtc agcggataaa cattcaccat tttgtcgaga tcgaaatcag cgtcctgctg
    60541 tttgatgacg tctttgccca tcgtcgacat attgctgccc ggaagtactg tgcaaccgct
    60601 tatcaaggtt actgacacca ataatggcat caatttcatt ttggatttca tcattgttta
    60661 tttatcactt tggcagagta attatcctgt gcactattaa tagcaatatc gccatgcaca
    60721 tttaccttgc agttaattga ataaaaattt aactggcatc agtcctaaaa aaattgattt
    60781 catccgcagg ctattgacag aataattcag actggtcttt caggcatcca gacacgctac
    60841 cgcccctggc tttttagcca ccaacacact gatttagctt aatttttcaa accctctctg
    60901 catacaatcg ttgatgagaa agggttattg cggaaattaa cttccgaata taaggtgaca
    60961 ttatggtaat tgaatattgg ctctccaata atgcaggagg aagtgttaca gctaacggaa
    61021 tagcaggcaa ggtaacaatt cggcaattgg ctatttttaa gaattatatt aagtgtcatt
    61081 caatatggtt tttaggagtt tctttaggtt gacaatattt aatattgcgc ctccacatgc
    61141 gatatttctt aaataatgtt ttattattac cacttttaat tcagggataa tgtgaggtta
    61201 ttaccctcaa ataatatgag ataaatagtg ctgcaacatt gcatttttgc cccgatttat
    61261 ccatgatcga attgtgacat ttgtcataca acgaataggt tttgtactta ctatggaatg
    61321 gattgccgat ccgtctatct gggccgggtt aatcacgctg attgtgatcg aactggtcct
    61381 cggcattgat aacctggtct ttattgccat cctcgccgaa aaactaccgc cgaggcagcg
    61441 cgaccgcgca cgggttaccg ggctgctgct ggcgatgtta atgcgcctgt tactgctggc
    61501 gtcaatctcc tggctggtca ccctgactca accgctgttc agcttccgct cgtttacctt
    61561 tagcgcccgc gacttaatca tgctgtttgg tggtttcttc ctgttgttca aagccacgat
    61621 ggagctgaac gaacggctgg aagggaaaga cagcaataat cccacacaac gcaaaggtgc
    61681 gaagttctgg ggcgtggtga cgcaaattgt ggtactggac gccattttct cactcgactc
    61741 agtgattacc gccgtcggga tggtcgacca tttactggtc atgatggccg ccgtggttat
    61801 cgctatcagc ctgatgttga tggccagcaa gccgttaacg caatttgtta acagtcaccc
    61861 gacgatcgtt attctctgct taagcttcct gttgatgatt ggctttagcc tggtggcaga
    61921 aggtttcggc ttcgtcattc cgaaaggcta cctgtacgct gccattggtt tctcggtgat
    61981 gatcgaggcg ctcaatcagt tggctatctt taaccggcga cgttttcttt ccgctaacca
    62041 gacgctgcgc cagcggacta ccgaagcggt aatgcgcctg cttagcgggc aaaaagaaga
    62101 tgcggaactg gacgccgaaa ccgcgtccat gctggtggat catggtaacc agcagatctt
    62161 taatccgcag gaacggcgga tgattgagcg ggtacttaat cttaaccagc gtaccgtcag
    62221 cagcattatg acgtcgcgcc acgatattga gcatatcgat ctcaacgcgc cggaagaaga
    62281 aatccgccag ttgctggagc gaaatcagca tacgcggctg gtggttaccg acggtgatga
    62341 cgcagaagat ttgctcggtg ttgttcacgt tatcgacctg ctacaacagt ctctgcgcgg
    62401 cgaaccgctc aacctgcggg tgttgattcg ccagccgctg gtgttcccgg aaaccttgcc
    62461 gttgttacct gccctggagc agttccgtaa tgcccgcacg cactttgctt ttgtggtgga
    62521 tgagtttggc tcggtggaag ggattgtgac attaagtgac gtcactgaaa ccattgtcgg
    62581 taacttaccg aacgaagtgg aagagatcga cgcccgccat gatattcaga agaatgcgga
    62641 cggttcctgg acggcgaatg gtcatatgcc gctggaagat ctggtgcaat atgtgccgct
    62701 gccgctggat gaaaaacgtg aatatcacac cattgccggg ctgttgatgg aatatttaca
    62761 gcgtattcca aaacctggcg aagaagttca ggtgggggat tatttgctta aaacgttgca
    62821 ggtggaaagc catcgcgtgc agaaggtgca gattattccg ctgcataagg atggcgagtt
    62881 ggagtacgag gtgtgatggt atcggtcatt ttacccaata gccgaaagga ataagatgag
    62941 ggaaccgcgc cagcggcgat ttaccgccgg gagcccgggt tgccagggtg gtggcgattg
    63001 agccaccctg gcacgttcac cggcttgagc gttccatagt agcgaggaac atcaagtgaa
    63061 cggaacagcc ttcgatctta catattcaga atataatgat gccgccacga acgcctgatg
    63121 cgacgcttta cgcgtcttat caggcctaca ggactggtca cctggtaacc aacatcttct
    63181 ccagcaactt cttcacatcc ttgccattgc gggaatcttt attccgctcg gcccagtcat
    63241 tcaggcgacg tttcgcttcg tcctgtagat gtttgcgcag taactgatcc acttgcaggc
    63301 tgtaattgag ttgctgccag ttgccataaa cccgcagcgg tactggcgtt tctttcagga
    63361 aatcaatcag tttactttcc ccgttccagc cacccacgac ccgaatatca aactgggtat
    63421 cgcaggtttg atctgccaga ttcaacatgc cttcccctgt cagcgccagc acaggcgatt
    63481 gaccttgcat gtcgtttaac gtcacgacgc catccttcaa cgtcaaatcg gtggtaaagc
    63541 ggtcaagccg cgtcacgtta tcaaagtttt cagcggcctt cacatcaccg ccattacgct
    63601 ctaccgcttg ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg tcggtcattt
    63661 cgacatgtgc ctgtccttgc cagttgtggc ggaatgcgtc ggcatctatg tcagcaccgg
    63721 agaaatcacc agccagtgac atttttccgg tcaacgaaat cggatagtta aacgccttca
    63781 gaatggtacc aatctcaacg ttttccagcc gtggctggaa gtttatccgc ggatttattg
    63841 atgtcgcgtc cagcgtgccc ggcagtgaaa cctgtccacc gttaagtttg ccctgcagtt
    63901 gagtaatttc cagcaaaccc gacttgttgg tcatttgcgt ggcaacatct gtaaaattca
    63961 ttccgcgcca gcgcacgtta ctggcctgca acaaaatatc agccgtaaag ccttgcagtc
    64021 cctgataggc cggttcatca atacgcgaag aaatgaccgg gcgcggcaac gttgattggc
    64081 tctgcccctg ctgcgcggca ccgttttcac cattcgcggt ttcattaagc gggatgaggt
    64141 tgtcaagatt cagttgcggg aattgcagtc tcagctgcca ttccggtttc tctgtcagcg
    64201 tgacctgtgc ttgcccgctc agcgtactgt cattggcggt caagctgatt tggttaaatg
    64261 aaaggcgttt atgtgactcc tgccactggg cctggaaact cccctgcccc tgaatacctt
    64321 gttttggtaa atcggcaccc tgcaactgcc agttaatttg ttcaatagcc gccgttaaat
    64381 catgcggata atcagaagca tccaccgtac cgttaaggga tattgtgaga tcgcgctgat
    64441 cgcgattaac ccgcccggag aactcaaatg agccacgatg ttggggatct tgttccattt
    64501 gcaggcggat attgcggatt gtcacctgct cgtcatcttc atgctggaac accagcacgc
    64561 tgtccgccac cttaagactg gatatatcaa acgaccatcc gcgatcgtct gacagatccg
    64621 gcaaggtatt gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg
    64681 gcgtcagttg gatcactgcc ccttttagca tcacctgctt aacgctcagt tgatgactca
    64741 gtagtggtaa aagcgccacg tccagacgca tgttgtcggc gcgaaccagt ggctggcttg
    64801 cgccctgggc ggtgagagac attcgcccgg agaggatact aagctgcggc cagacgtgcc
    64861 aacgcagtgg cccgtcgagc tgcaattgat aaccgctacg tgcagcaact tgcttgacca
    64921 tatagtcgcg gaaatcattc ggattcacca gcaacactaa cacagataac ccggccacca
    64981 gcacgaccag gagtatcatc agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc
    65041 tgaattagtc tttatcgatt cggctggcta ccgcgccctg ctggttgcga tatttcgcat
    65101 cttcacggcg gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac
    65161 caattaacat gcccggacgc agcgccagcg gcagcttacc ggagttgtag aactccagca
    65221 caatgcaacc agaccagccc ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca
    65281 gacgcgccaa agaggaacgc ccgtccagcc agcccaccag atcggctggc agcgtcaccg
    65341 actccagcgt caccgccagc gccagctctc ctgggtgaag atagaacgcc tcgccctcgt
    65401 cgagaacgat ctcatcgctc atcacgcggt caagcgcggc gctcacttca tctttgggac
    65461 cgctcagatc gataaacgct gccgtgtgac cacggaaggt acgaaattta ttgcccaggc
    65521 gtacatccac cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg atcgacaaac
    65581 ggccttcatc aagccaggct tcaatatctc ggtcacacag acgcatggca ttttctcctt
    65641 tcgcgcatca ctcccttaac gccaattacg tcaagggcat accaggttat cactgaacgg
    65701 tacacaattt atcaagctta ttcaaagaac tgacttattt tcgctttcaa tatatcgatc
    65761 gcgatgcggt ttttcccgcc gcgcggcacg ataatgtccg catattgttt agaaggctca
    65821 atgaattgca ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac ggaatccatt
    65881 gaacgcccac gctcgttaac gtcacgcttg atgcggcgca tcaggcagat atccagcggg
    65941 gtatcaacga aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa
    66001 atgccttcga gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt catacgcgta
    66061 tgttcaacat agctgtaaac cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa
    66121 tgctcaagca gcaggctgtg atccatcgcg ctgggatggt cgtagttggt cttaacgcgt
    66181 tcttccatcg acagatggct ttgatctttg taatagcagt cttcgggaat tacgccgatg
    66241 tgttcatcac cgacttgctc acgtaattca cgataaaggg tactggcaat aagactcttg
    66301 ccggaagccg atgcgccagc gataccgata atgacgcact gatgagactg atcagtcata
    66361 tatttagcga cctgattaac ctggatgtta ggaagggggc gacgaagcgc caaacgcggc
    66421 aattataggg atttcatccg tctgatacca gtcgaatagc gttgccgcgc gctcagagtt
    66481 aattgttgac aaagaattcc cggtggcaaa ttacgttgat cagttttatg taaggtaaaa
    66541 aatgttatac gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat
    66601 ataaaatgta aaacttttgt actagcataa acacagaaac gaatactggc gacctggtct
    66661 tgcggataaa gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc
    66721 gctgcttcac ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga
    66781 gctttcgcag tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt
    66841 ggcgttttat cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gttcgctggg
    66901 aaatatcgcc gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac
    66961 catcaatatt gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta
    67021 taaccccttg caaaatctgg ctgactggct gcgtctggca ctcggcagcg ccattgttcc
    67081 gcctctgttg gggggtgttc tggttgtcct gctgacgccc ggagacgatc ctctcagggc
    67141 atttttgata tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt
    67201 gttatttaaa ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct
    67261 gctcacgtta gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc
    67321 ttttactttc attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc
    67381 ctttttgatc ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc
    67441 cctgcttgct acgccgcgta cgtacctgat gagccatatg ccgtggctac cgtttttgct
    67501 gatcctgctg cccgccaaca tcatgaccat ggtgatgtat gcctttcgtg cggaacgcaa
    67561 acacatttcc gaaagcgaaa cccgctttcg gaacgcgatg gaatattccg ctatcggtat
    67621 ggcgttagtg ggcaccgagg gacaatggct gcaaaccaac aaagcgctct gccagtttct
    67681 cgggtacagt caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga
    67741 tctcaataaa gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc
    67801 aatggaaaaa cgctactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc
    67861 actggtgcgc cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa
    67921 cgagctaaaa cgcaccgaac aggtcaatca gcaactgatg gagcgcatca cgctggctaa
    67981 cgaagcgggc gggattggca tctgggagag ggagttgaag ccgaatgttt ttagctggga
    68041 taagcggatg ttcgagctgt atgaaattcc tccacatatc aaaccgaact ggcaggtgtg
    68101 gtacgagtgc gtgctgccgg aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt
    68161 gcaatcacgc tcgcccttta aactggaatt tcgcattacc gtaaaagacg gtattcgcca
    68221 tatccgcgcc ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gtctcctcgg
    68281 catcaatatg gatatgaccg aagtgaaaca gcttaacgag gcattgtttc aggaaaaaga
    68341 gcgcctgcac attacgcttg attccatcgg tgaagccgtg gtctgtattg atatggcgat
    68401 gaaaattacc tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc
    68461 gttaggtgtt ccgctcctga cggtgttgca tattactttt ggcgacaacg gaccattaat
    68521 ggagaacatt tacagtgccg acacctcacg ttccgcaatc gaacaagatg tggtgttgca
    68581 ctgtcggagc ggcggcagtt acgacgtgca ttacagtatt acgccgttaa gtactctgga
    68641 cggcagcaat attggttcgg ttctggtgat tcaggacgtc accgaatcac gcaaaatgct
    68701 gcgccagctg agctacagcg cctcccatga tgcactgacg catctcgcca accgcgccag
    68761 ttttgagaaa caactgcgta tcctgctgca aacggtaaac agtacgcatc agcgacatgc
    68821 cctggtgttt atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc
    68881 gggcgacgct ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gctccagtga
    68941 cgtgctggcg cggctcggcg gtgatgaatt tggtctgctg ctgccagact gcaatgttga
    69001 aagcgctcgt tttatcgcta cacgtattat cagtgctgtg aatgactatc actttatatg
    69061 ggaaggccgg gtacatcggg taggtgccag cgccgggatt accttgatcg atgacaacaa
    69121 tcatcaggcg gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg
    69181 tggacggggc cgggtgacgg tttacgaacc acagcaagct gccgcacata gcgaacgggc
    69241 ggcgatgtcg cttgatgaac agtggcggat gattaaagag aatcagttga tgatgatcgc
    69301 ccacggtgtc gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga tttcgcttaa
    69361 gctctggagt tgcgaaggcg aaattattga tgaacaaaca tttcgtcgta gcttcagcga
    69421 tccggcactt agccatgctc ttgaccgacg ggtattccac gaatttttcc agcaggccgc
    69481 caaagcggtt gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag
    69541 tagcgccacg ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg
    69601 gttattacat ctgattattc cggctgaagc gattttagat cacgcagaaa gcgtgcaaaa
    69661 actgcagctg gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt
    69721 caactcgctg aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa
    69781 cgtgcagggt aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg
    69841 actcgggatg aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc
    69901 tggcatcggc gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt
    69961 gctggtgaat agcagttatt tcgcgattaa ctgagagagc gtttgcccat tgcctgatgc
    70021 gacgctaacg cgtcttatca tgcctacaaa tcgctcattc cccagaccgg ataaggcgct
    70081 cgcaccgcat ccggcgacca acgtcatgct tcgtctggtt gccagccttc cgtataccag
    70141 atatgcaaca gcgcataaga acgccagggc ttccagcgct cggcataacg gcggatttgt
    70201 gccggtgtca ttcccggaaa tcgccgttta atcagataat catccggcag aaaaacatct
    70261 ttcgcctgcc agccacgcaa agcaaaataa ttcgccgtcc agcgcccgat acccggaaaa
    70321 gtttgcagcg ttttcatcgc ctgctccaca tcgcccggaa ttgtcattgg taagctgccc
    70381 tccagcgccg catttgccag atgaatcagt gcctctgccc gtttcaacgg catgcctaac
    70441 gcttttaatg cctgcgggtc ggctgctgcc agccgctgag gcgtggggaa gcagatatac
    70501 tccggaaaat catccagccg ttcgccataa agctgtacca ctttggcagt caattttgcc
    70561 gccatcgcca cgctcaccag ttggcctaaa atcgcccgca caccctgctc aaaagcatca
    70621 atacagccgg gtaaacgcaa tccgggccgc cccgcgccta actttcccaa cgcaccgtta
    70681 acaatctgcg ggttacattg cagatcaagc aggcggctca ttttcgccag acactctgcg
    70741 gcaacaggtt ctaaacctgc acttaaattt atgtgcagag tatggcgggc tatatccgga
    70801 atagcagtca ctacgccgcg atattcgccc accgccagac tacgggcata ataattgtcc
    70861 gcgaccgttt ccacaccgct caccgcacgg gcggcgagaa atcccaacat ccacgaccag
    70921 tcatacggcg gctgccagtt cagggtatac atcgcatctc cttgttaatc cgctttcagc
    70981 ataaacgtta ttcagacgtt acgctttgct ttcatattcc ggttgtcgcg acagcaacat
    71041 ttcgctaaag tcacgcccct tcttcaccgg catggggatt aattcgtgtt tattggtttt
    71101 gattacggta cagcaaactg ttcagtggcg gtcatgcgtg acggtaaacc gcaattactg
    71161 aaaatggaaa acgacagcac gctgctgcct tcaatgcttt gcgcgccaac gcgtgaatcg
    71221 gtaagcgaat ggctgtaccg ccatcatgat gttccggcag acgacgatga aacgcaggcg
    71281 ctgttacgtc gggcgattcg ttataaccgc gaagaagata tcgatgttac ggcgaaaagc
    71341 gtgcagttcg gtctttcctc actggcacag tacattgatg atccagaaga agtgtggttt
    71401 gtgaaatcac caaaatcgtt cctcggtgcc agcggcttaa aaccgcagca ggtagcgctg
    71461 tttgaggatc tggtctgcgc aatgatgttg cacattcgcc agcaggcgca ggcgcagctg
    71521 ccagaagcga ttactcaggc ggtgattggt cgcccgatca acttccaggg gctgggcggc
    71581 gatgaagcaa acgcccaggc gcaagagatt ctggaacgcg cggcgaagcg tgccggattc
    71641 agggacgtgg tattccagta cgagccgatc gcggctgggc tggattacga agccaccttg
    71701 caggaagaaa aacgggtgct ggtggtggat atcggcggtg gtacgactga ctgttcattg
    71761 ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg aagccagtct gctgggtcac
    71821 agtggttgtc gtattggcgg taacgatctg gatatcgccc tggcgtttaa aaacctgatg
    71881 ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg ccctgccgat cctgccgtgg
    71941 tggaatgcgg ttgccatcaa cgacgtacct gcgcagagtg atttctacag tagtgccaac
    72001 ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac cggaaaaagt ggccctgtta
    72061 cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc gtagcgcaga agagtgcaaa
    72121 attgctcttt caagcgtagc ggaaacccgc gcctcactgc cgtttatcag caatgaactg
    72181 gctacgctga ttagccagcg agggctggaa agcgccctca gccagccact ggcgcggatt
    72241 ctggaacagg tgcaactggc cctggataac gcccaggaaa aaccggacgt tatctacctg
    72301 accggcggta gcgcccgttc tccgcagatt aaaaaagcgc tggcagaaca gttgccgggc
    72361 attccgattg caggcggcga tgactttggc tccgtcaccg ccgggctggc acgctgggcg
    72421 gaagtggtgt ttcgttaatt gaaaaatggc tcagcagcgg cttaaatctg cctgcgcctg
    72481 agccagtttt tctgctgcct gacgtaacgg ttccatcaac ggcatggtcc gtgctggagc
    72541 atgaaggcgg aatcgctgca actctgtcgc gccgactgtc aacgccgatt ccagccgatg
    72601 tcgtttggcg gtcattttcg ccattaccgc ttgtctgtct gctggcgtta tcgcttcatt
    72661 cggcctgaac acaaaacggc gttcacagtt cgctttccag tcgataaccg cctgggtcag
    72721 atggtcgcca aaccctttca cttgcttaac gccacgacgg gtaacatctg cggctgtttc
    72781 aataccaaaa gaccgtaacg ccgctttacg cgcagggcca acgccgggaa tagaggcaac
    72841 atcaataaaa aatccctcca gaaacttctg cttctgccgt tcccttgcag tgtcatgaag
    72901 tgctgccaga gcgcgtttct cttcttccgg taacccgaga atttcgtcct tcattttttc
    72961 gagcatcgtc cgtttggcga taaaaccttc caggccgcct acctgctgga tctggcggac
    73021 taaatggtca tagtccattt tcgcacgatt aaatgcctca cggcgttgct gaacttctgc
    73081 tttgtacgct ttgcttgtca aactgccaat aatccagata gccgccagca caggaaccaa
    73141 aataatataa cgcggttctg cctgaaggcc gcaaagcagc agcgataatg ctgacagtgc
    73201 gatctcaagc agaatgatgt attcgcgccg taacaggcct aaaggaagcg gcctgccagt
    73261 cggttggaaa tgatcgggta atggcagttg caatgctggc ggtgctactg acgccatcac
    73321 catcgcccag acttttgcca gcacaaaatc cccgccggtg gtaatgacct cttcgccgag
    73381 atcaataaaa tagataacac cttgattatc cagcgcacac catgggcagt cagccaaatg
    73441 accgggataa acatgcattg ccgaaacggt acatttcttt aactgttggc gtagagaatc
    73501 cagtgctgat acccacgctt tagccgtcgg acgcccggtt gccacgccac tttccgtaaa
    73561 cgcctgctga aacatggctt caacatcact cggtaacatc gatagcggaa tcgatcgtgg
    73621 cggcggtttt aaaccacgtc gctgattatc tgacgcgtag gcataacgga aatgggtaat
    73681 atccgtctcc agcgcattac ccgcatcaga gataagcggc acaccagaat aaggatgccg
    73741 gccaccaaac aagacgtgaa aaatcagcaa cgcaaggcca aaattatcgt gattttctgt
    73801 gcgttcaaag ccgacaaatg acgacaaggt ttgtagctct ggcggcgtaa aatgcgacac
    73861 gccgacttcg cataaatgca gtgtgccatt ggcgttaatc tgaaaggagt cgctatcaat
    73921 caacaccact ttgctgtcgc gacctaccat aaaactgttc tggtttacgt cacccacgac
    73981 gtgcccgtgc tcgtgaaccg tagcaaaaga tgaagcaata ttgcgcgcaa catagagtag
    74041 aaaatcccac gcacaatgag ggtaacgttg acgacgatgt gccgggctat agatcatatg
    74101 aatcggttct ttaccagaaa cttttggcat cataaaaccg ataacttttc cgcctcgtcc
    74161 accgtgaagc gttgcctgcg gccaggcgac ataattcaac aattgcgcgt cagctgtcgc
    74221 agccataaag gcaagtttgt cctgttttaa ggcgggtggc ggcgtgtgat aaatcttggc
    74281 gacgctatcg acaaactcat tgatatcata aaccgcgcct tcaccgcctt tgcccagttc
    74341 acggcctggc gtaacgcact caccagtagc agtatataaa gtgggtttca tggtaaccac
    74401 agcgccagtg ccaacgtttt atcatcatcc gtacgctcgt ttaccgccgg gctggacaaa
    74461 aactgcttta acaattcagg taataaatcg agttgttcct gcgttgctga cgccagtcca
    74521 ttgaaaaacg gggtaaagaa aggcacatga ggagaattat ccagcatatt taacgccagc
    74581 cgctgaatac catccgtaaa tgctgcaact ttatgcgcac gcccagtgct ggtgaaagtt
    74641 tccagtctgg aaacggcatc ttcgtcggta atgaagtgcg tcatgttggc atattcaccc
    74701 accatcggcg tgagaggcaa ttgcagaccg tgacccaaat ctaccaccac gccgccatcg
    74761 ccaatctgca taatcaaggt gccgtcaggc gatgaaatca atcccagaaa cgtgcaggca
    74821 aaatcgcgaa ctgctaatgc tttggcttct gcctcggcaa aaagtcgttg acgaatagtc
    74881 agtaccatat ttgtggcgag gacatcatta agccccaatt caccgtcctg tactttttgc
    74941 accataaacg ccatcgcttc attgacggcg agcgtcgcgc cttctcctcc ctgcgaaacg
    75001 ctacctgcgc catcagcaag gaacatcacc agtaatggtt gctggtcgtt taaccagaca
    75061 acctgcatct ggcaggcatc ctgacaaggc aaatcagcac taatgtgcga tgttccaaca
    75121 gcggatgcat aaaccagacg ccagctcaca ctgacgtcca gccttttggc gcttccagca
    75181 caacttccgt tcccggcgtg gagcgggaaa ccgaacgcag cgaactggat aaccagctaa
    75241 acagttcacg gaattgtaat ccctgcagtg gcaaaggctg acgaacgctg atttgtgcca
    75301 gagtcttcat atccgccccc tgaacaccga tggaaaagaa ggcaaacttc ttatcttctt
    75361 ccccctgaaa cactttgttg gcggcagcct gccactcatc ggttggagcg ccatcggtga
    75421 tcaggaaaat ccacgggcga taatacgaaa taccatttgc gcggtactca cgtttcctct
    75481 cttcaaccat atccagggct ttggtaatgg cggcccccat aggcgtatcc ccctgggcaa
    75541 acaggatggg cgggaaaaag ttagcggcgc tggtaaaggg ttgttccaca tgcaccgggc
    75601 cgaacgtcac aatgccgagt tccactcttt ttaaagccag cggatcagca agcagttcat
    75661 cacgaaaggt aaccaatccg gcgttaagtt cattaatagg tctgccgctc atcgagccag
    75721 aaacatccag cagcaaaatg caggggcaac gtggttccgg gttactggca aaatcgctgg
    75781 tggcaaacgt gatttgttcg ctcataagaa atcctttctt agagaattat cgaggtccgg
    75841 tatcataccg attgcaataa tgaaataaga gcaaacgaag aaactttcag aattaggccc
    75901 gtcggcgcag aaaattttgc tattcgcctc gcaagaacgg agagccgtcg attgacacta
    75961 agggcggagt gacataattt caggagtgag ggttagggag aggtttcccc ctccccctgg
    76021 tgttcttagt aagcctggaa gctaatcact aagagtatca ccagtatgat gacgtgcttc
    76081 atcataaccc tttccttatt aaaagccctc ttctccggga gagggtttcc cgtttcagcg
    76141 tcccgctgaa atcgtcggct tacctccttt cgccctgaat gcagtctatc gctaacgcgt
    76201 gaacaaaata gtttcatctg tgattattgc gtaacgactc gcaaagaata tttgctggca
    76261 ggatcgcaga ctacaaagcc tgcggattga caatcttatc gtgaaggcat aatttcagga
    76321 gtgagggtag agcggggttt cccccgccct ggtagtctta gtaagcgggg aagcttatga
    76381 ctaagagcac cacgatgatg agtagcttca tcatgaccct ttccttatta aggccccttc
    76441 ctcgggaggg gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttac ctcctttcgc
    76501 cctgtatgca gtctatcgct gacttgcgga taaaatagtt tccagtgtga ttattgcgta
    76561 acgtctcgca aagaatattt gctggcagga tcgcagactg caaagcctgc agattgacaa
    76621 tcttatcgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    76681 agccttagta agctggccag ctaatgacta agagcaccac gatgatgagt agcttcatca
    76741 tgaccctttc cttatttatg gccccttcct cgggaggggc tttcccgttt cagcgtcccg
    76801 ctgaaatcgg cggcttacct cctttcgcca tgcaagcagt ctatcgctaa cgcgtagata
    76861 aaatagtttc ctgtgttatt actggatgcg tgctcgcaaa tgtgcccgtc attcagacga
    76921 ttcccgacag tgtttcataa ttcctccatt tttctccctt attggctggc tacactagta
    76981 tcattccgcg aaacgtttca ggaagagaaa ctcttaacga tgaaaggcag ttataaatcc
    77041 cgttgggtaa tcgtggtggt tatcgccgcc atcgccgcat tctggttctg gcaaggccgc
    77101 aatgactccc agagtgcagc cccaggggcg acgaaacaag cgcagcaatc gccagcgggt
    77161 ggtcgccgtg gtatgcgttc cggcccatta gccccggttc aggcggcgac cgccgtagaa
    77221 caggcagttc cgcgttacct caccgggctt ggcaccatta tcgccgctaa tactgttacg
    77281 gtgcgcagcc gcgtggacgg tcaactgatg gcgttacatt tccaggaagg ccagcaggtc
    77341 aaagcaggcg atttactggc agaaattgac cccagccagt tcaaagttgc attagcacaa
    77401 acccagggcc aactggcaaa agataaagcc acgcttgcta acgcccgccg tgatctggcg
    77461 cgttatcagc aactggcaaa aaccaatctc gtctcccgtc aggagctgga tgcccaacag
    77521 gcgctggtca gtgaaaccga aggcaccatt aaggctgatg aagcaagcgt cgccagcgcg
    77581 cagttgcaac tcgactggag ccgcatcacc gcaccagtcg atggtcgcgt tggtctcaag
    77641 caggttgatg ttggtaacca aatctccagt ggtgatacca ccggaattgt ggtgatcacc
    77701 cagacgcatc ctatcgattt agtctttacc ctgccggaaa gcgatatcgc tactgtagtg
    77761 caggcgcaaa aagccggaaa aacgctggtg gtagaagcct gggatcgcac caattcgaag
    77821 aaattaagtg aaggcacgct gttaagcctc gataaccaaa tcgatgccac aaccggtacg
    77881 attaaagtga aagcacgctt taataatcag gatgatgcgc tgtttcccaa tcagtttgtt
    77941 aacgcgcgca tgttagtcga caccgaacaa aacgccgtag tgatcccaac agccgccctg
    78001 caaatgggca atgaaggcca ttttgtctgg gtgctgaata gcgaaaacaa ggtcagcaaa
    78061 catctggtga cgccgggcat tcaggacagt cagaaagtgg tgatccgtgc aggtatttct
    78121 gcgggcgatc gcgtggtgac agacggcatt gatcgcctga ccgaaggggc gaaagtggaa
    78181 gtggtggaag cccagagcgc caccactccg gaagagaaag ccaccagccg cgaatacgcg
    78241 aaaaaaggag cacgctcctg atgcaggtgt tacccccgag cagcacaggc ggcccgtcgc
    78301 gcctgtttat tatgcgtcct gtcgccacca cgctgctgat ggtggcgatc ttactcgccg
    78361 ggattatcgg ttatcgcgcc ctgcccgttt cggcgctgcc ggaagtggac tatccgacca
    78421 ttcaggtggt cacgctctac ccaggtgcca gcccggatgt catgacctct gccgttaccg
    78481 cgccgctcga acgccagttc gggcagatgt ctggcctgaa acagatgtcg tcgcaaagtt
    78541 ccggcggtgc gtcagttatc actttgcagt tccagctaac attaccgctc gatgtcgccg
    78601 agcaggaagt gcaggccgcg attaacgctg cgaccaactt gttgccgagc gatctgccta
    78661 acccgccggt ttacagcaaa gtgaacccgg cagatccgcc gatcatgacg ctcgccgtca
    78721 cctcaaccgc catgccgatg acgcaagtgg aagatatggt ggaaacccgc gtcgcgcaga
    78781 aaatctcgca gatttccggc gtcggcctgg tgacgctttc cggcggtcag cgtccggctg
    78841 ttcgcgtcaa acttaacgct caggcgattg ccgccctcgg cctgaccagc gaaaccgtgc
    78901 gcaccgccat taccggcgct aacgttaact cggcaaaagg tagcctcgac ggcccttccc
    78961 gtgcggtcac gctttccgcg aacgaccaga tgcaatccgc cgaagagtat cgccagctaa
    79021 tcatcgccta ccagaacggc gcgccaattc gtctgggcga tgtcgcaact gtagagcaag
    79081 gtgcagaaaa cagctggctc ggcgcgtggg cgaacaaaga acaggccatt gtgatgaatg
    79141 ttcagcgcca gcccggtgct aacattatct ccaccgccga cagcattcgg cagatgctgc
    79201 cacagctcac tgagagtctg ccgaaatcgg tgaaggtgac agtgctttcc gatcgcacca
    79261 ccaatatccg cgcatccgtc gatgatactc agtttgaatt gatgatggct atcgcgctgg
    79321 tagtcatgat tatctacctg tttttgcgca atattccggc gaccatcatt cccggtgttg
    79381 ctgtaccgct gtcgttaatc ggcactttcg cggttatggt gtttctcgat ttttcaatca
    79441 ataacctgac actgatggcg ttaactatcg ccaccggatt cgtggtcgat gacgccatcg
    79501 tggtgatcga aaacatttcc cgctatatcg aaaaaggcga aaaaccgttg gcggcggcgc
    79561 tcaagggcgc aggtgaaatc ggctttacca ttatctcgct gaccttctca ctgattgcgg
    79621 tgttgatccc actgctgttt atgggcgata tcgtcgggcg actgttccgc gaatttgcta
    79681 ttaccctggc ggtagcgatt ttgatctcag cggtggtgtc gctgaccctg acaccgatga
    79741 tgtgcgcgcg gatgctcagc caggagtcgt tgcgtaaaca gaaccgcttc tcccgtgcct
    79801 cggaaaaaat gttcgacagg ataatcgccg cctatggtcg tggactggcg aaagtgctga
    79861 atcatccgtg gctgacctta agcgtggcac tcagcacgct gctgcttagc gtgctgctgt
    79921 gggtgttcat tccgaaaggt ttcttcccgg tacaggacaa tggcattatt cagggcactt
    79981 tgcaggcacc gcaatccagc tcctttacca atatggccca gcgacaacgc caggtcgcgg
    80041 acgtgatttt gcaggatccg gcagtgcaaa gcctgacctc atttgttggc gttgatggca
    80101 ctaacccgtc gctgaatagt gcacgtttac aaatcaacct caaaccgttg gatgaacgtg
    80161 atgatcgggt gcaaaaagtc atcgcccgtc tgcaaacggc ggtagataaa gtgccgggcg
    80221 tcgatctctt cctgcaacca acgcaggatc tgactattga tactcaggtc agccgcaccc
    80281 agtaccagtt taccttgcag gccacctcac tggatgcgct cagtacctgg gtgccacagt
    80341 tgatggaaaa actccagcaa ctgccacagc tttctgatgt ctccagcgac tggcaggacc
    80401 aagggctggt ggcgtatgtc aatgttgatc gcgacagcgc cagccgtctg gggatcagca
    80461 tggcggatgt cgataacgcc ctgtacaacg cgtttggtca gcgactgatt tccactattt
    80521 atactcaggc taaccagtat cgcgtagtgc tggagcacaa caccgaaatt accccgggcc
    80581 tcgcggcgct ggataccatt cgcctgacca gtagcgacgg cggcgtggtg ccgttaagct
    80641 caattgccaa agttgaacag cgttttgcgc cgctttccat caaccatctg gatcagttcc
    80701 cggtaacgac catctccttt aacgtgccgg ataactattc gctgggcgat gcggtgcagg
    80761 agattatgga caccgaaaag acgctgaatc tgccggtgga tatcaccacg caattccagg
    80821 gcagcaccct cgccttccag tcggcgctgg gcagcactgt ctggctgatt gtcgcggcgg
    80881 tggtggcgat gtatatcgtg ctcggcattc tgtacgagag ctttattcac ccgatcacca
    80941 ttctctcgac gctacccacc gcaggggtcg gcgcgctgct ggcattaatg attgccggta
    81001 gcgaactgga tgtgattgcg attatcggca ttattttgct gatcggtatt gtgaagaaga
    81061 acgccatcat gatgatcgac ttcgcgctgg ctgctgagcg cgagcaaggc atgtcgccgc
    81121 gcgatgcaat ctaccaggct tgtctgttgc gttttcgtcc gatcctgatg accaccctgg
    81181 cggctctgct tggcgcgctg ccgctgatgt tgagtaccgg ggtcggcgcg gaactgcgtc
    81241 gtccgttagg tatcggcatg gtcggcggtc tgattgtcag ccaggtgctg acgctgttca
    81301 ccacgccggt gatttatctg ctgttcgacc gcctggcatt gtggaccaaa agtcgctttg
    81361 ctcgccatga agaggaggcg taagtgaagt tttttgccct cttcatttac cgcccggtgg
    81421 cgacgatttt actgtcggtc gccattaccc tgtgcggcat actgggcttc cgtatgctgc
    81481 cggtcgcccc gctgccgcag gtcgattttc cggtgattat ggtcagcgcc tcgctgcccg
    81541 gtgcgtcacc agaaacaatg gcgtcttccg ttgccacgcc gctggagcgc tcgcttgggc
    81601 gcattgccgg ggtcagcgaa atgacctcca gcagttcgct cggcagcaca cgtattattt
    81661 tacagtttga ttttgaccgg gacatcaacg gcgcagcgcg tgatgtgcag gcggcgatca
    81721 acgctgcaca aagtttgctg cccagtggga tgcccagccg tccgacctat cgcaaagcga
    81781 acccgtcgga tgcgccaatt atgatcctca cgctgacatc cgatacttat tcgcagggtg
    81841 aactgtacga tttcgcctcg acgcagctgg ctccgacgat ttcgcaaatc gacggtgttg
    81901 gtgatgtcga tgtcggaggc agctcactgc ccgccgtacg cgtcgggctg aatccgcagg
    81961 cgctgtttaa tcagggcgtg tcgctggacg acgtacgcac cgccatcagc aatgccaacg
    82021 tgcgtaaacc gcagggcgcg ctggaagatg gcactcaccg ctggcagatc cagaccaatg
    82081 atgagctaaa aaccgccgct gaatatcagc cgttgattat tcactacaac aacggcggcg
    82141 cggttcgtct gggcgatgtg gcgacggtga ccgactcagt gcaggatgtg cgcaacgccg
    82201 ggatgaccaa cgccaaaccg gctattttac tgatgatccg caaactgccg gaagccaata
    82261 ttatccagac ggttgacagc atccgggcaa aattaccgga gttgcaggaa accattccgg
    82321 cggcgattga tctgcaaatt gcccaggatc gctcccccac cattcgcgcc tcgctggaag
    82381 aagtcgagca aacgctgatt atctcggtgg cgctggtgat tctggtggtg tttttattcc
    82441 tgcgctcggg tcgcgccact attattcccg ccgtttcggt gccggtttcg ctgattggta
    82501 cgtttgcggc gatgtacctg tgcggattca gtctcaataa cctttcgtta atggcgctca
    82561 ccatcgctac tggtttcgtg gtggatgacg ccatcgtggt gctggaaaac attgcacgtc
    82621 atctggaagc gggaatgaaa ccgttgcaag ccgcactgca aggtactcgc gaagtcggtt
    82681 ttacggtgct gtcgatgagt ctgtcactgg tggcggtgtt cctgccgctg ctgttgatgg
    82741 gcggattgcc gggccgactg ttacgcgagt ttgccgtgac gctttctgtc gccattggta
    82801 tttcgttgct ggtttctctg acattaacgc caatgatgtg tggctggatg ctgaaagcca
    82861 gcaagccgcg cgagcaaaag cgactgcgtg gttttggtcg catgttggta gccctgcaac
    82921 aaggctacgg caagtcactg aaatgggtgc tcaatcatac ccgtctggtg ggcgtggtgc
    82981 tgcttggcac cattgcgctg aatatctggc tgtatatctc gatcccgaaa accttcttcc
    83041 cggagcagga cactggcgtg ttgatgggcg ggattcaggc ggatcagagt atttcgtttc
    83101 aggcgatgcg cggtaagttg caggatttca tgaaaattat ccgtgacgat ccggcagtgg
    83161 ataatgtcac cggctttaca ggcggttcgc gagtgaacag cgggatgatg tttatcaccc
    83221 tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt ctgcgcgtaa
    83281 aactggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaggat attcgcgttg
    83341 gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac ctggcggcac
    83401 tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa ctggcggacg
    83461 tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac cgcgacacca
    83521 tggcacggct gggaatcgac gtgcaggccg ccaacagcct gttaaataac gccttcggtc
    83581 agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg atggaagtgg
    83641 atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc aataacgaag
    83701 gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaacgct ccgctgtcgg
    83761 tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg accggaaaat
    83821 cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt gtgccttcga
    83881 cggtgcgcgg cagttttgcc ggaacagcgc aggtgttcca ggagacgatg aactcacagg
    83941 tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc ctttacgaga
    84001 gttacgtaca cccactgacg attctctcca ccctgccctc ggcgggcgtt ggtgcgctgc
    84061 tggcgctgga gctgttcaat gccccgttca gcctaatagc cctgataggg atcatgctat
    84121 taatcggcat cgtaaagaaa aacgccatta tgatggtcga ttttgcgctc gaagcccaac
    84181 ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg cgttttcgcc
    84241 cgattatgat gactaccctg gcggcgctgt ttggcgcgct gccgctggtg ttgtcgggcg
    84301 gtgacggctc ggagctgcgg cacctcctgg ggatcaccat tgtcggtgga ctggtaatga
    84361 gccagcttct gacgctgtat accacgccgg tggtgtatct ctttttcgac cgtctgcggc
    84421 tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagaa cttcccgaca
    84481 gcacccgctg gcaattgtgg attgtggctt tcggcttctt tatgcagtcg ctggacacca
    84541 ccatcgttaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt ccgttgcata
    84601 tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc gccagcggct
    84661 ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg ctgtttactc
    84721 tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg gcacgcgcgt
    84781 tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg atgaaaatcg
    84841 taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt caggtcggtc
    84901 cgctgctcgg tccggcgctc ggcggtctgc tggtggagta cgcgtcgtgg cactggatct
    84961 ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgctg ttaatgccga
    85021 actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg gcggttggca
    85081 tggctgtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg ccgctggcga
    85141 ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg cacgccagaa
    85201 ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt tcgctgggcc
    85261 tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg acaccggttt
    85321 tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg atcccgatgg
    85381 tacttggcag catgggaatg aagcgaattg tggttcaggt ggtgaatcgc tttggttatc
    85441 gtcgggtact ggtagcgacc acgctgggtc tgtcgctgat caccctgttg tttatgacta
    85501 ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa gggatggtca
    85561 actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg gacaatctgg
    85621 cgagcagcgg caacagtctg ctgtcgatga ttatgcaatt gtcgatgagt atcggcgtca
    85681 ccatcgccgg gctgttgctg ggactttttg gttcgcagca tgtcagcgtc gacagcggca
    85741 ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc gcccttccgg
    85801 cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt tcgcggcgaa
    85861 aaaggagcgc gcaatgaagt tctggcgacc tggtattacc ggcaaactgt ttctggcgat
    85921 tttcgccacc tgcattgttt tgctgatcag tatgcactgg gcggtgcgta tcagttttga
    85981 gcgtggcttt attgattaca tcaagcatgg taatgaacag cgattacaac tgttaagtga
    86041 tgcgcttggc gagcagtatg cgcagcatgg caactggcgc ttcctgcgca acaatgatcg
    86101 ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata aacccggccc
    86161 gggtatgcca ccgcacggct ggcgtaccca gttctgggtg gttgatcaaa acaacaaagt
    86221 gctggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca ttctggtcaa
    86281 cggtgcggaa gttggcgcgg tgatcgcctc ccccgttgag cggttaacgc gcaatactga
    86341 tatcaatttc gataaacaac agcggcaaac cagctggttg attgtcgccc tggcaacgtt
    86401 actcgcggca cttgccactt ttctgctggc gcgcggttta ctggcaccgg taaaacgact
    86461 tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa cgcccaccag
    86521 tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag cttgccagca cactggagaa
    86581 aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc gtacgccatt
    86641 agcggtgctg cgcggtgaac tggaagctat tcaggatggc gtgcgcaaat tcacgccgga
    86701 gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg acgatctcca
    86761 tcagttgtcg atgtctgatg aaggcgccct cgcctatcaa aaagcaccgg tagatttgat
    86821 cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc gtggcctgaa
    86881 actgcaattt tccctgccag acagtattac cgtatttggc gatcgcgacc gtttaatgca
    86941 gttattcaat aatttacttg aaaacagcct gcgctacact gacagcggcg gcagcctgaa
    87001 aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca gtgcgccagg
    87061 tgtcagtgac gagcagctac aaaaattatt tgaacgtttt tatcgcaccg aaggttcccg
    87121 caaccgcgcc agcggtggtt ccgggctggg gctggcgatt tgcctgaaca ttgttgaagc
    87181 acataatggc cgcattattg ctgcccattc gccttttggc ggggtaagca ttacagtaga
    87241 gttaccgctg gaacgggatt tacagagaga agtatgaccg agttaccaat cgacgaaaac
    87301 acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct cattgattat
    87361 ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt actgccgtat
    87421 gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg caccgatggc
    87481 ttgacgctgt gccgggaaat tcgtcgtttt tctgacattc ctatcgtgat ggtgacggca
    87541 aaaatcgaag agatcgaccg cctgctcgga ctggagattg gcgcagatga ctacatctgc
    87601 aagccgtaca gcccacggga agtggtagcg cgcgtcaaaa ccattttgcg ccgttgcaaa
    87661 ccgcagcgcg aattgcagca acaggatgcc gaaagcccgt tgattatcga cgaaggtcgt
    87721 tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt tcgtctgttg
    87781 aaaacgctct ctcatgaacc aggaaaagtg ttctcccgcg agcaattgct caatcatctt
    87841 tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa aaacctgcgc
    87901 cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta cggcgttggt
    87961 taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatga ctttgttagc
    88021 cggagatggc gttcagacca gatccggcaa cattatccca cgcatggtca gcaaactga
//
LOCUS       DAJTVK010000003        79580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000003 DAJTVK010000000
VERSION     DAJTVK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 79580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.003"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="OO372_000214"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="OO372_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="OO372_000215"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="OO372_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCR6339832.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="OO372_000216"
     CDS             2354..2758
                     /locus_tag="OO372_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCR6339833.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="OO372_000217"
     CDS             2811..2921
                     /locus_tag="OO372_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339834.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            3157..3201
                     /locus_tag="OO372_000218"
     CDS             3157..3201
                     /locus_tag="OO372_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339835.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            3454..3777
                     /gene="iraM"
                     /locus_tag="OO372_000219"
     CDS             3454..3777
                     /gene="iraM"
                     /locus_tag="OO372_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6339836.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO372_000220"
     CDS             complement(3880..5130)
                     /gene="icd"
                     /locus_tag="OO372_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6339837.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            5302..5955
                     /gene="rluE"
                     /locus_tag="OO372_000221"
     CDS             5302..5955
                     /gene="rluE"
                     /locus_tag="OO372_000221"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6339838.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            5965..6426
                     /gene="nudJ"
                     /locus_tag="OO372_000222"
     CDS             5965..6426
                     /gene="nudJ"
                     /locus_tag="OO372_000222"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6339839.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            6480..7586
                     /gene="mnmA"
                     /locus_tag="OO372_000223"
     CDS             6480..7586
                     /gene="mnmA"
                     /locus_tag="OO372_000223"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6339840.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            7622..8263
                     /gene="hflD"
                     /locus_tag="OO372_000224"
     CDS             7622..8263
                     /gene="hflD"
                     /locus_tag="OO372_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6339841.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            8267..9637
                     /gene="purB"
                     /locus_tag="OO372_000225"
     CDS             8267..9637
                     /gene="purB"
                     /locus_tag="OO372_000225"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6339842.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9805..10476
                     /gene="phoP"
                     /locus_tag="OO372_000226"
     CDS             9805..10476
                     /gene="phoP"
                     /locus_tag="OO372_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6339843.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            10476..11936
                     /gene="phoQ"
                     /locus_tag="OO372_000227"
     CDS             10476..11936
                     /gene="phoQ"
                     /locus_tag="OO372_000227"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6339844.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            12012..13133
                     /gene="roxA"
                     /locus_tag="OO372_000228"
                     /gene_synonym="ycfD"
     CDS             12012..13133
                     /gene="roxA"
                     /locus_tag="OO372_000228"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6339845.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO372_000229"
     CDS             complement(13182..14408)
                     /gene="pepT"
                     /locus_tag="OO372_000229"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6339846.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            14658..15794
                     /gene="potA"
                     /locus_tag="OO372_000230"
     CDS             14658..15794
                     /gene="potA"
                     /locus_tag="OO372_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6339847.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15778..16635
                     /gene="potB"
                     /locus_tag="OO372_000231"
     CDS             15778..16635
                     /gene="potB"
                     /locus_tag="OO372_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6339848.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            16632..17426
                     /gene="potC"
                     /locus_tag="OO372_000232"
     CDS             16632..17426
                     /gene="potC"
                     /locus_tag="OO372_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6339849.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            17423..18469
                     /gene="potD"
                     /locus_tag="OO372_000233"
     CDS             17423..18469
                     /gene="potD"
                     /locus_tag="OO372_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6339850.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO372_000234"
     CDS             complement(18625..19446)
                     /gene="cobB"
                     /locus_tag="OO372_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6339851.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO372_000235"
     CDS             complement(19462..20373)
                     /gene="nagK"
                     /locus_tag="OO372_000235"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6339852.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO372_000236"
     CDS             complement(20402..21646)
                     /gene="lolE"
                     /locus_tag="OO372_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6339853.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO372_000237"
     CDS             complement(21646..22347)
                     /gene="lolD"
                     /locus_tag="OO372_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6339854.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO372_000238"
     CDS             complement(22340..23539)
                     /gene="lolC"
                     /locus_tag="OO372_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6339855.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            23801..24874
                     /locus_tag="OO372_000239"
     CDS             23801..24874
                     /locus_tag="OO372_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6339856.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            25002..28448
                     /gene="mfd"
                     /locus_tag="OO372_000240"
     CDS             25002..28448
                     /gene="mfd"
                     /locus_tag="OO372_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6339857.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            28592..29548
                     /gene="ldtC"
                     /locus_tag="OO372_000241"
                     /pseudo
     CDS             28592..29548
                     /gene="ldtC"
                     /locus_tag="OO372_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO372_000242"
     CDS             complement(29631..29888)
                     /gene="bhsA"
                     /locus_tag="OO372_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR6339858.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30129..30761
                     /gene="comR"
                     /locus_tag="OO372_000243"
     CDS             30129..30761
                     /gene="comR"
                     /locus_tag="OO372_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6339859.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(30823..31362)
                     /locus_tag="OO372_000244"
     CDS             complement(30823..31362)
                     /locus_tag="OO372_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6339860.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO372_000245"
     CDS             complement(31589..32893)
                     /gene="ndh"
                     /locus_tag="OO372_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6339861.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33302..33844)
                     /locus_tag="OO372_000246"
     CDS             complement(33302..33844)
                     /locus_tag="OO372_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6339862.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(33867..34892)
                     /gene="nagZ"
                     /locus_tag="OO372_000247"
     CDS             complement(33867..34892)
                     /gene="nagZ"
                     /locus_tag="OO372_000247"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6339863.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(34903..35727)
                     /gene="thiK"
                     /locus_tag="OO372_000248"
     CDS             complement(34903..35727)
                     /gene="thiK"
                     /locus_tag="OO372_000248"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6339864.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(35708..36349)
                     /gene="lpoB"
                     /locus_tag="OO372_000249"
     CDS             complement(35708..36349)
                     /gene="lpoB"
                     /locus_tag="OO372_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6339865.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36363..36740)
                     /locus_tag="OO372_000250"
     CDS             complement(36363..36740)
                     /locus_tag="OO372_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6339866.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(36743..37102)
                     /gene="hinT"
                     /locus_tag="OO372_000251"
     CDS             complement(36743..37102)
                     /gene="hinT"
                     /locus_tag="OO372_000251"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6339867.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            37436..39625
                     /gene="fhuE"
                     /locus_tag="OO372_000252"
                     /pseudo
     CDS             37436..39625
                     /gene="fhuE"
                     /locus_tag="OO372_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(39685..41118)
                     /gene="ptsG"
                     /locus_tag="OO372_000253"
     CDS             complement(39685..41118)
                     /gene="ptsG"
                     /locus_tag="OO372_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6339868.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(41413..42210)
                     /locus_tag="OO372_000254"
     CDS             complement(41413..42210)
                     /locus_tag="OO372_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6339869.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42221..43225)
                     /gene="holB"
                     /locus_tag="OO372_000255"
     CDS             complement(42221..43225)
                     /gene="holB"
                     /locus_tag="OO372_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6339870.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43222..43863)
                     /locus_tag="OO372_000256"
     CDS             complement(43222..43863)
                     /locus_tag="OO372_000256"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6339871.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(43853..44875)
                     /gene="yceG"
                     /locus_tag="OO372_000257"
     CDS             complement(43853..44875)
                     /gene="yceG"
                     /locus_tag="OO372_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6339872.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(44878..45687)
                     /gene="pabC"
                     /locus_tag="OO372_000258"
     CDS             complement(44878..45687)
                     /gene="pabC"
                     /locus_tag="OO372_000258"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6339873.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(45807..47048)
                     /gene="fabF"
                     /locus_tag="OO372_000259"
     CDS             complement(45807..47048)
                     /gene="fabF"
                     /locus_tag="OO372_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6339874.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47136..47372)
                     /gene="acpP"
                     /locus_tag="OO372_000260"
     CDS             complement(47136..47372)
                     /gene="acpP"
                     /locus_tag="OO372_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6339875.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(47583..48317)
                     /gene="fabG"
                     /locus_tag="OO372_000261"
     CDS             complement(47583..48317)
                     /gene="fabG"
                     /locus_tag="OO372_000261"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6339876.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48330..49259)
                     /gene="fabD"
                     /locus_tag="OO372_000262"
     CDS             complement(48330..49259)
                     /gene="fabD"
                     /locus_tag="OO372_000262"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6339877.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49275..50228)
                     /gene="fabH"
                     /locus_tag="OO372_000263"
     CDS             complement(49275..50228)
                     /gene="fabH"
                     /locus_tag="OO372_000263"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6339878.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50296..51366)
                     /gene="plsX"
                     /locus_tag="OO372_000264"
     CDS             complement(50296..51366)
                     /gene="plsX"
                     /locus_tag="OO372_000264"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6339879.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(51447..51620)
                     /gene="rpmF"
                     /locus_tag="OO372_000265"
     CDS             complement(51447..51620)
                     /gene="rpmF"
                     /locus_tag="OO372_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6339880.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(51672..52193)
                     /gene="yceD"
                     /locus_tag="OO372_000266"
     CDS             complement(51672..52193)
                     /gene="yceD"
                     /locus_tag="OO372_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6339881.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            52392..52976
                     /gene="yceF"
                     /locus_tag="OO372_000267"
     CDS             52392..52976
                     /gene="yceF"
                     /locus_tag="OO372_000267"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6339882.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53088..54047)
                     /gene="rluC"
                     /locus_tag="OO372_000268"
     CDS             complement(53088..54047)
                     /gene="rluC"
                     /locus_tag="OO372_000268"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6339883.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            54620..57805
                     /gene="rne"
                     /locus_tag="OO372_000269"
     CDS             54620..57805
                     /gene="rne"
                     /locus_tag="OO372_000269"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6339884.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(58000..58953)
                     /gene="flgL"
                     /locus_tag="OO372_000270"
     CDS             complement(58000..58953)
                     /gene="flgL"
                     /locus_tag="OO372_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR6339885.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(58965..60608)
                     /gene="flgK"
                     /locus_tag="OO372_000271"
     CDS             complement(58965..60608)
                     /gene="flgK"
                     /locus_tag="OO372_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR6339886.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(60674..61615)
                     /gene="flgJ"
                     /locus_tag="OO372_000272"
     CDS             complement(60674..61615)
                     /gene="flgJ"
                     /locus_tag="OO372_000272"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6339887.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(61615..62712)
                     /gene="flgI"
                     /locus_tag="OO372_000273"
     CDS             complement(61615..62712)
                     /gene="flgI"
                     /locus_tag="OO372_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6339888.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(62724..63422)
                     /gene="flgH"
                     /locus_tag="OO372_000274"
     CDS             complement(62724..63422)
                     /gene="flgH"
                     /locus_tag="OO372_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6339889.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(63475..64247)
                     /gene="flgG"
                     /locus_tag="OO372_000275"
                     /pseudo
     CDS             complement(63475..64247)
                     /gene="flgG"
                     /locus_tag="OO372_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(64419..65174)
                     /gene="flgF"
                     /locus_tag="OO372_000276"
     CDS             complement(64419..65174)
                     /gene="flgF"
                     /locus_tag="OO372_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR6339890.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65194..66399)
                     /gene="flgE"
                     /locus_tag="OO372_000277"
     CDS             complement(65194..66399)
                     /gene="flgE"
                     /locus_tag="OO372_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6339891.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(66424..67119)
                     /gene="flgD"
                     /locus_tag="OO372_000278"
     CDS             complement(66424..67119)
                     /gene="flgD"
                     /locus_tag="OO372_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6339892.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67131..67535)
                     /gene="flgC"
                     /locus_tag="OO372_000279"
     CDS             complement(67131..67535)
                     /gene="flgC"
                     /locus_tag="OO372_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR6339893.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(67539..67955)
                     /gene="flgB"
                     /locus_tag="OO372_000280"
     CDS             complement(67539..67955)
                     /gene="flgB"
                     /locus_tag="OO372_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6339894.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            68110..68769
                     /gene="flgA"
                     /locus_tag="OO372_000281"
     CDS             68110..68769
                     /gene="flgA"
                     /locus_tag="OO372_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6339895.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVQDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            68845..69138
                     /gene="flgM"
                     /locus_tag="OO372_000282"
     CDS             68845..69138
                     /gene="flgM"
                     /locus_tag="OO372_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6339896.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            69143..69559
                     /gene="flgN"
                     /locus_tag="OO372_000283"
     CDS             69143..69559
                     /gene="flgN"
                     /locus_tag="OO372_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6339897.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(69599..71134)
                     /gene="murJ"
                     /locus_tag="OO372_000284"
     CDS             complement(69599..71134)
                     /gene="murJ"
                     /locus_tag="OO372_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6339898.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71244..72167)
                     /locus_tag="OO372_000285"
     CDS             complement(71244..72167)
                     /locus_tag="OO372_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6339899.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72169..72816)
                     /locus_tag="OO372_000286"
     CDS             complement(72169..72816)
                     /locus_tag="OO372_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6339900.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(72827..73411)
                     /gene="rimJ"
                     /locus_tag="OO372_000287"
     CDS             complement(72827..73411)
                     /gene="rimJ"
                     /locus_tag="OO372_000287"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6339901.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            73647..74855
                     /gene="mdtH"
                     /locus_tag="OO372_000288"
     CDS             73647..74855
                     /gene="mdtH"
                     /locus_tag="OO372_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6339902.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            74919..75566
                     /gene="grxB"
                     /locus_tag="OO372_000289"
     CDS             74919..75566
                     /gene="grxB"
                     /locus_tag="OO372_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6339903.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            75700..76260
                     /locus_tag="OO372_000290"
     CDS             75700..76260
                     /locus_tag="OO372_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6339904.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76366..77412
                     /gene="pyrC"
                     /locus_tag="OO372_000291"
     CDS             76366..77412
                     /gene="pyrC"
                     /locus_tag="OO372_000291"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6339905.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            77486..77731
                     /gene="dinI"
                     /locus_tag="OO372_000292"
     CDS             77486..77731
                     /gene="dinI"
                     /locus_tag="OO372_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6339906.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78021..78275
                     /gene="bssS"
                     /locus_tag="OO372_000293"
     CDS             78021..78275
                     /gene="bssS"
                     /locus_tag="OO372_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6339907.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            78390..79508
                     /gene="solA"
                     /locus_tag="OO372_000294"
     CDS             78390..79508
                     /gene="solA"
                     /locus_tag="OO372_000294"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6339908.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        19599 a        20912 c        19710 g        19359 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcac ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgttata taaaaaggca accataatgt ttagcaaatt ggcaccaagt
     3181 agcataaagg ctatgtttta attacaggat gttcagtcat ttgaatgtat aacattatag
     3241 ctaaacaaat ctaaaacgaa gtgaataatt tattgttttc actaaatctc attttgttta
     3301 acatccattg agattccttg ctttaaattt tgttttatat aagcaatcgt tttaattaat
     3361 ttattttttg aaggggtaat atactcatat gcaaaataaa gaaatgaaca tccaaatgaa
     3421 ctatattaaa taccgtggga taagacataa caaatgaagt gggtagtaat tgacacggta
     3481 attcaaccta catgcggcat atctttttca gccatatggg gtgatatgaa aaagatcatc
     3541 tggtatcaat ctactatatt tcttcctcct ggaagtatat ttacaccggt taagcttggt
     3601 attatcctta aggaaaaaga atatcctatt actatttata acattgcacc attcaacaag
     3661 aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag caaaataaca
     3721 aataaatgtt tacataatag ttgcattata aaaatatgcc catatgggct caagtaatgg
     3781 gcttcatata aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc
     3841 gtgttatacg cccgtttcaa tatttaactc atggagagat tacatgtttt cgatgatcgc
     3901 ttcaccaaac tctgaacatt tcagcagctt agcgccttcc atcagacgtt caaagtcata
     3961 agttacggtt ttcgcgttga tagcaccttc cataccttta acaattaaat cagccgcttc
     4021 agtccacccc atatggcgca gcatcatctc agcggagagg atgatagagc ctggatttac
     4081 tttgtcctga ccggcatatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc
     4141 gtcaccgatg tttgcgccag gggcgatacc gataccgcca acctgcgctg ccagggcgtc
     4201 agaaatgtag tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag
     4261 caggatctgt tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt
     4321 gttcgggttt ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaactcttc
     4381 acgcgccagc tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc
     4441 tttgtgcacc agagtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg
     4501 aaccagacgt ttggtgcctt cttccgaaca cggcttaata ccgataccac aatgttccgg
     4561 gaagcgaatt ttcttcaccc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc
     4621 ggcagagtct gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat
     4681 aaccatatcg gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac
     4741 cggacgcagg cagatgtaga gatccagttc ctggcgcagg gcaacgttca gagagcgaat
     4801 accgccacca acaggagtgg tcagcggacc tttaatggca acgcgatatt cacgaatcag
     4861 atcaagggtt tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc
     4921 ggtgtaaatt tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc
     4981 gtcgaccact ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta
     5041 agggataatc ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc
     5101 ttgtgccgga acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc
     5161 tcgtctgcca ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat
     5221 tttttgttaa tgatttgtaa ttggcttgtc aatgctaccc tattgtttgc cgtcatgaaa
     5281 gatgcgcgtt attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa
     5341 acttctttta gaaatcacca ggttaagcga ttcagctcgc aacgttctac taggcgtaaa
     5401 cctgaaaacc agcccacgcg tgtgatcctg ttcaataaac cctacgatgt tcttccgcag
     5461 ttcaccgatg aagccggacg caaaacatta aaagaattca tcccggttca gggcgtttat
     5521 gcagcaggtc gtcttgaccg cgataccgaa gggttactgg tgctgaccaa taacggcgcg
     5581 ttgcaggcgc gtttaaccca gccgggcaaa cgcaccggga aaatctatta tgtgcaggtg
     5641 gaaggtattc ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat
     5701 ggccctaccc tgcccgccgg cgcggagctg gttgacgaac cagcgtggtt atggccgcgg
     5761 aatccaccaa ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat
     5821 gaaggacgta atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga
     5881 ctgattcgtt atgcgatggg tgattacact ttggataacc ttgccaatgg cgaatggcga
     5941 gaagcaacag aataaggatg cacaatgttt aaaccgcacg ttaccgttgc ttgcgtagtg
     6001 cacgcagagg gtaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac
     6061 caacctgccg ggcatctgga agccgatgaa accttagtgg aagctgccgc ccgtgagcta
     6121 tgggaagaaa ccggcatcag cgcgcaaccg caacacttta ttcgtatgca tcagtggatt
     6181 gcgccagata aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc
     6241 ccgacccagc ctcatgacag cgatatcgac tgctgtcgtt gggtcagcgc cgaagaaatt
     6301 ttaaaggcgt caaatcttcg ttcgccgctg gtggcggaaa gtatccgttg ttatcaaagt
     6361 gggcaacgct atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc
     6421 atctaaaagg ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa
     6481 tgtctgaaac cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt
     6541 ctgcctggct gttgcaacaa cagggatatc aggttgaagg cctgtttatg aagaactggg
     6601 aagaagacga cggtgaggaa tattgcacag cggcagcgga tctggctgat gcccaggctg
     6661 tctgcgacaa gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca
     6721 atgtcttcga actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc
     6781 tgtgcaacaa agagatcaaa tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg
     6841 ccgattatat cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc
     6901 tgctgcgtgg tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg
     6961 agcagattgc gcaaagcctg ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga
     7021 ttgctgaaga tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca
     7081 ttggcgaacg taaattccgc gagttcctgg gccgttatct cccggggcaa ccgggcaaaa
     7141 tcattaccgt cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg
     7201 gtcagcgtaa aggtctgggt atcggtggca ccaaagaagg caccgaagaa ccttggtatg
     7261 tggtggacaa agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc
     7321 ggctgatgtc tgtcgggttg attgcccagc agttgcactg ggtcgatcgc gaaccattca
     7381 ccggcactat gcgttgcacg gtaaaaaccc gctatcgcca gaccgatatc ccttgcaccg
     7441 tcaaggcgct ggacgatgat cgcattgaag tgattttcga tgaaccggtt gccgccgtga
     7501 cgccgggcca gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg
     7561 agcagcgtct gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa
     7621 cgtggcaaag aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg
     7681 cctggtgcaa caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact
     7741 caacagtatt attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc
     7801 caacctgcgc gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gtcgccaggg
     7861 cttaaacgcc gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc
     7921 ctcagcgaaa ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct
     7981 cgaacacttc gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga
     8041 tgtgatcagc ccgcttggcc cacgcattca ggtcaccggt tcccctgctg tactgcaaag
     8101 cccacaagtg caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct
     8161 ctggcaccag gtcggcggcg gacgtctgca actgatgttt tctcgtaatc gcctgaccac
     8221 tcaggcaaaa caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc
     8281 actgaccgcc gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg
     8341 gattttcagc gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca
     8401 aaaactggcc gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg
     8461 ttaccttgat gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat
     8521 cgagcgtacc actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc
     8581 ggagatcccg gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga
     8641 tatcaataac ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc
     8701 gtactggcgt caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc
     8761 gctgctgtct cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc
     8821 aaacgtcgcc taccgtatgg agcgccagta ccgccagctt aaccaggtgg agatcctcgg
     8881 caaaatcaac ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga
     8941 ctggcatcag ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac
     9001 tacccagatt gaaccgcacg actacatcgc cgagctgttt gattgcgttg cacgcttcaa
     9061 caccattctg atcgactttg atcgtgacgt ctggggttat atcgccctta accacttcaa
     9121 acagaaaacc attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat
     9181 cgacttcgag aactccgaag ggaatctggg cctttccaac gcggtattac agcatctggc
     9241 aagcaaactg ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa
     9301 cctcggcgtg ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag
     9361 caaactggaa gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct
     9421 ggctgagcca atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct
     9481 gaaagagctg actcgtggta agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg
     9541 tctggcgttg ccagaagaag agaaagcccg cctgaaagcg atgacgccgg caaactacat
     9601 tggtcgcgcc atcaccatgg ttgatgagct gaaataaact cgtatcagtg ccggatggcg
     9661 atgctgtccg gcctgcttat taagattatc cgctttttat tttttcactt tacctcccct
     9721 ccccgctggt ttatttaatg tttaccccca taaccacata atcgcgttac actattttaa
     9781 taattaagac agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta
     9841 cgtcaccacc ttaaagttca gattcaggat gctggtcatc aggtcgatga tgcagaagat
     9901 gccaaagaag ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc
     9961 ggattgccag acgaggacgg tctgtcactg attcgccgct ggcgtagcaa tgatgtttca
    10021 ctgccgattc tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt
    10081 gctggtgctg atgattacgt gactaaacca tttcatattg aagaggtgat ggcgcgaatg
    10141 caggcattaa tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct accaccgttt
    10201 caggttgatc tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg
    10261 ttcgaataca ccattatgga aacgttgata cgcaataatg gcaaagtggt gagcaaagat
    10321 tcgttaatgc tccaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta
    10381 ctgatgggac gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt gattactacc
    10441 gttcgcggcc agggctattt gttcgaattg cgctgatgaa aaaattactg cgtctttttt
    10501 tcccgctctc gctgcgggta cgttttctgt tggcaacggc tgcggtagta ctggtgcttt
    10561 cgcttgccta cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt
    10621 ttcggctgtt acgtggcgag agcaatctgt tctataccct tgcgaagtgg gaaaacaata
    10681 agttgcatgt cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt
    10741 atgatgagaa cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaagatga
    10801 tccagcctga ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata
    10861 ccagcctctt gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag
    10921 atgatgacga cgcggagatg acccactcgg tggcagtaaa cgtctacccg gcaacatcgc
    10981 ggatgccaaa attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct
    11041 atatggtctg gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc
    11101 tgctgtgggt cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc
    11161 gcgaactgga agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca
    11221 gtctggtacg aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc
    11281 gtacgacgct caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta
    11341 cgctgcgttc tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg
    11401 agcaaatcag ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg
    11461 gcgggacatt gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct
    11521 cagcgctgaa caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag
    11581 agatcagctt tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata
    11641 atgcctgtaa atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc
    11701 tctatattgt ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt
    11761 tcgaccgtgg tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag
    11821 cccgcgaaat caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg
    11881 gtgcgcggat ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat
    11941 gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat accagcctgt
    12001 ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt gaacgtcact
    12061 ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac ccgatctctc
    12121 cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg gtcagtcacc
    12181 aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat ctcggtgaaa
    12241 ccaactggtc attactggta caggcagtga accactggca tgagccgacc gccgcgctga
    12301 tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatt tctttttctg
    12361 tacctggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc attcagggta
    12421 ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac tgcccacatc
    12481 cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg gagcctggtg
    12541 atattcttta tattccgcca ggattcccgc atgaaggcta cgcgctggaa aatgcgatga
    12601 actattccgt gggctttcgc gcgccaaata cgcgggaact gattagtgga tttgccgatt
    12661 atgtgctgca acgtgaactg ggcggcaact actacagcga tcctgatgtt ccacctcgcg
    12721 ctcatcctgc ggatgttctg cctcaagaga tggataaact gcgtgagatg atgctcgaat
    12781 tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc cagtcacgtc
    12841 atgaactgga tatcgcgccg ccagaaccgc cttatcagcc agatgaaatc tacgatgcgc
    12901 tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg
    12961 acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg gatgcactcg
    13021 ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat ccgtcattcc
    13081 tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt cttcgaaggg taagtttgga
    13141 tttaagccgg atgcggcatc acatgccgca tcccttttcg cttacttccg ttgcgccgtt
    13201 aactcggcaa tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac
    13261 tcatgcttac catgatagtt gtaaccgcca gtgaacaagt tcgggcacgg taatcccata
    13321 aacgacaact gcgcgccgtc ggtaccaccg cggatcggtt tcagttccgg ttcaatatcg
    13381 cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctct
    13441 cgcatattgt agtaactgtc ttcaatcacc agttcaatgt agcaatcagg atgtaaccct
    13501 ttgcccactt ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg
    13561 tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa cggtgccttt catgctcgcc
    13621 aggtgataga aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc
    13681 gcatgaatac gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccgga
    13741 tgaacattgt taccgacaat tttgatattg accgatgcgg cgttgaagtt ttcaaactcc
    13801 agttcgccta cgccaccacc gtcaacagtg taagcccaac gggcatcgaa ggcatcaaca
    13861 tcaaaatgtt tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca
    13921 ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaatacct
    13981 gctttgtcat cggcacctaa caaggtttta ccatcggtgg taatcaacgt ctgacccagt
    14041 agctgatgca gcaccgggaa cataaccggt gataaaactt catcgccgat acccagcgca
    14101 atatcgccac cgcgatagtt ttcaacaatt tgcggattaa catttttgcc gctgcaatcc
    14161 ggtgaggtat ccacatgaga aataaagcca atcgccggga tatcgccagg gacgttagcc
    14221 ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatttct
    14281 tcgagctgct ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt
    14341 ctcacccctg cttttgattg ggtatccaga gatacgtagt tcaaaaatcg ctcaagtagt
    14401 ttatccatgt agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa
    14461 atattgcgtc aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat
    14521 gaatataaag ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta
    14581 gaattaccgc cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag
    14641 ccatccgttg cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt
    14701 caccgctggt gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc
    14761 agctggatct gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg
    14821 gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca
    14881 tgctggataa cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt
    14941 tccaaagcta cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc
    15001 gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg
    15061 tgcaactgga aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac
    15121 gcgtcgccat tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc
    15181 tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc
    15241 gtaagcttgg tattaccttc gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt
    15301 cagacaggat tgtggtgatg cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa
    15361 tttacgaaga gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatc aatatgttta
    15421 acgccactgt catcgaacgt ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg
    15481 aatgtaatat ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc
    15541 gcccggaaga cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg
    15601 gttacgttcg tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa
    15661 atggcaagat ggtgatggtc agcgaattct tcaatgaaga cgaccctgac tttgaccact
    15721 ctctcgacca aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg
    15781 aagaacacaa gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg
    15841 tttgtctttc tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc
    15901 agtttcgtca aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat
    15961 tttgaagtgc tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg
    16021 ctgggctacc cgtttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg
    16081 ctgtttctgc tgattgttcc cttctggacc aactcactga ttcgcatcta cgggctgaaa
    16141 attttcctca gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac
    16201 acaccaattc gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg
    16261 ctaccgttta tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt
    16321 gaggcggcgc gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca
    16381 ctgacgatgc cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg
    16441 ttctatgtat ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag
    16501 gtccagttcc tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact
    16561 atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag
    16621 gtggaactcg aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt
    16681 acctgtatat cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca
    16741 tcaactggca gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt
    16801 tacaggcagc gcagcattca ctaacaatgg cggtgttttc ggcgacattt gctacgctta
    16861 tcggttcact gacggcagtt gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta
    16921 gcggaatgct gtttgtggtg atgatgtcac cagatatcgt gatggcgatt tctctgctgg
    16981 tgctgtttat gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca
    17041 ccttctgcct gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc
    17101 ggatgctgga agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca
    17161 ttctgccact ggcaatgcca gcggtggcgg cgggctgggt gttaagcttt accctgtcga
    17221 tggacgatgt ggtagtttct tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa
    17281 aaatttattc gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat
    17341 tactggtgct gtcgctggtg atggttattg ccagccagct tattgctcgt gataaaacga
    17401 aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc
    17461 tctggcactg ggcatgagcg ccgctcacgc cgatgacaac aacacgctgt atttctacaa
    17521 ctggaccgag tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa
    17581 ggctatctat tcgacttacg agtcgaacga aaccatgtac gccaagctga aaacgtacaa
    17641 agacggtgcc tatgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga
    17701 agggatgatc cagaagatcg acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga
    17761 catgctcaac aagccgtttg acccgaataa cgactactcc attccgtata tctggggtgc
    17821 gacggcgatt ggcgttaacg gtgatgcggt ggatcctaaa tctgtcacca gttgggcaga
    17881 tctgtggaag ccagagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt
    17941 ccagatggcg ctgcgcaaac tgggctactc cggtaacacc accgatccga aagagattga
    18001 agctgcatat aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa
    18061 cccggctaac ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc
    18121 tttcgttgca cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat
    18181 tttctggatg gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa
    18241 attgatcaac ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta
    18301 tccaacgcca aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac
    18361 gctctacccg gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc
    18421 cagcagcatt tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg
    18481 ggcggtggta ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag
    18541 taaagtttta tcccgctctt tcgtcggtat tgtggtcccc ttgtaggcct gataagcgta
    18601 gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa caaactcagg
    18661 caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga cctgacttgg
    18721 ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa caaacccagc
    18781 cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca tcgacaacgc
    18841 catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt gcgggcgcaa
    18901 gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc cggtccagtc
    18961 gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca tatgaatgac
    19021 attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca gcaaaaagcg
    19081 atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag gctgaatttc
    19141 tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt caggatcgcg
    19201 atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca ggccatctgc
    19261 ggcgcgaaag gtacgaatac ctgattccgc agaaattcct gcccctgtca gtacgagtac
    19321 tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac gctgacgcaa
    19381 acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac cccgacgcga
    19441 cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac cacgcattcc
    19501 tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag gtaagagatg
    19561 acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata agccaccacc
    19621 aatgacgacc aggtcagagt caacgatggt caggatattt cccagacaaa ccgctaataa
    19681 atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat aaagcgcaat
    19741 aatttcggga gcctgcaacg gttgatgata atagtgttgc cagagccacg caaagccccg
    19801 acctgataga taattttcga tacaaccaat ttgaccacag ccgcagcggc gtaacgggaa
    19861 atccagcccc atcatggtta acgcatcaac cggcagacgc atatggccaa actcgccggt
    19921 aatgtagctt ttaccggtaa tcggtttacc gttgaaaatc agcccgccgc caacgccggt
    19981 gccgagaatt aaccccatca ccagtggata ttgcgtaaat tcatcatccc aggcttctga
    20041 aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac gtgcgctcag
    20101 gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca gcgtaccatc
    20161 ttctgtttcc ggcattcccg gaataccgat gccaacagaa cctttacagc caaaacgttg
    20221 gtcagcttcg gctaccagct cgcatactgc atctaaaaat gcgtcatagc tgtcacgcgg
    20281 tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc caagcgcaat
    20341 ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta atgtactgcc
    20401 tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc gccggatacc
    20461 aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg tccagccagt
    20521 gcaattccga tggcaggaag tcaataaaat agatatcgct ggagaggaac tgatgaccga
    20581 tcagcttttc aatccgctca ataatcgggg taagttgcag tgaaacaact acgccgataa
    20641 tcacaccaca caggctaccg aacagccctg ccagcaatcc ataccagaca aagatggcgc
    20701 gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca ctcttgtctt
    20761 tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc accagtacca
    20821 tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag ccgtaagtac
    20881 caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg cgtaccagct
    20941 tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg gaacccatat
    21001 caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg agttgaccac
    21061 tcaactgcaa aataccggca atgtgcaaac gcacacgttt tggctgcatc agtttatgct
    21121 cgggattcga gttgggaatc atgatcgaca cccaatcgcc ctgcttcact ttcagcgcat
    21181 ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg cgccaggcat
    21241 ccccctgaac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg ttaacgccct
    21301 tcacctgaat tgcgcgcaga ttggtaccac tttctaccag tccggtgaaa ttgatatacg
    21361 gcgcagcggc ggcaataccc ggcacttttt gcacgttatc cagtgcttcc tgccagttag
    21421 tccatggctg atttaccgcc tcaatttcac cgtgcggtac cacagcaaga atgcggttat
    21481 tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc gccacgccga
    21541 gggcaatgcc aatggtagaa atgacagaga ttagcgacac catgccgccg cgtcgccgtc
    21601 cgcgactaaa acgcaggcca atcaataacg ataaaggcat cgccattact ccgcccccat
    21661 caggctcagt tccgccgtca gacgtccatc acgcatttcc agttggcggc tcatacgttt
    21721 cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc gattcaattc
    21781 cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg taggctcatc
    21841 cgcaagtacc agacgcggat tattgaccag cgcacgggca atcgccacac gctggcgttc
    21901 gccgccagaa agttcagatg ggcggtgatt cgcacgatgc tccagcccca ccgcttttaa
    21961 catctccagc gcacggctgt tgatttcagc gggctttttc ttgccaatca gcagtggcat
    22021 agccacgttt tccagggcag taaaatccgg cagcaggtgg tgaaactgat aaataaagcc
    22081 cagcttctgg ttgcgcagtt ctgctttcgc cgccgaagac agtttgctca tcggttgacc
    22141 gttaaagatc acatcgccgg aggttggtgt atccagcccg cccagcaggt gcagcaaggt
    22201 acttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc cgacgctgaa
    22261 actgacattg tgcagtacat cggtttgcac actgccttcc tgatagcgtt tgcacaggtt
    22321 gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga gtggcggcag
    22381 cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc agcgcaataa
    22441 caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc aggacgccga
    22501 ttatcggcat cagattattt aactggctgg caagcagtgc gccaagcgcc gctccgagga
    22561 tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg atttgtcgcg
    22621 gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg cttctccatt accatcagcc
    22681 ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc agcaagccca
    22741 tcatattttt ttccatgcgt acggcctgga acagctcgcc tttacggtca cgccagtcct
    22801 gccatttgct gccttcaggc agtttttgct gacttaaaga gtcaactttc agcggctcat
    22861 ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga cgcgaggcat
    22921 cctcaatatt caccagcatt tcatagccat cgacttcact gttggcggcg aaattgccaa
    22981 tcacattgaa caggcgctgg cttggaatac gccccatcgg cgtgaactgg ctggcagatg
    23041 gtaccatcac gcggatttga tcaccgcgat taacgcctag ctgtgaggca agttgttcgc
    23101 cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg accagatacg
    23161 gcgtaagtgg atctttttgc gccgggtcga taccgagcat caccccgacc gccacgctgc
    23221 gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta acgccgtcca
    23281 gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca gaagagagaa
    23341 ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag ccgttcatca
    23401 ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta gaaagccagg
    23461 agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg ccaataaata
    23521 gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac gagtatataa
    23581 gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta atctgaaaag
    23641 cccccgtaaa tcaatcggct aaattgccat gttttatcat caatcttgcc gcctgatctt
    23701 aatctgaaaa atcatccaat acagacagat acccattaca cctcgggtca taacccgcag
    23761 gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa aagagctatg
    23821 gattaaccag atcaaagggt tatgtatttg tctggtggtg atttatcact cggtcattac
    23881 cttttatccg catatgacca cttttcagca tccgttatcg gaagtcctga gtaaatgctg
    23941 gatctatttc aatctttacc ttgcaccctt tcgtatgccg gtttttttct ttatatccgg
    24001 ctatttgatt cgccgctata tcgacagcgt accgtgggga aattgtctcg ataaacggat
    24061 ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg cagtggctgg cgttaagtgc
    24121 gctgaaccag tggctggcac ctgagcgcga tttaagcaat gcctccaatg ccgcttatgc
    24181 cgattccacc ggtgagttcc tgcacgggat gatcaccgcc agcaccagct tgtggtatct
    24241 gtatgcgtta attgtctatt tcgtggtatg taaaattttt agccgtctgg cgctgccact
    24301 attcgccttg tttgtactgc tgagtgtggc ggttaatttc gttcccacgc cgtggtgggg
    24361 aatgaacagt gtgatccgca atttgcctta ttacagcctt ggcgcatggt ttggcgcaac
    24421 aataatgacc tgtgttaaag aggtgccgtt gcgccgccat ctgctgatgg cttctttgct
    24481 taccgttctg gcggtcggtg cctggttgtt tactatctcg ctgctgttgt cgctggtatc
    24541 gattgtggtg atcatgaagc tgttttatca gtacgagcaa cgattcggta tgcgctccac
    24601 cagcctgctg aatgtgattg gctccaacac cattgctatc tacaccaccc atcgcattct
    24661 ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac gcagcacgct ggtcgccgca
    24721 agtcgaactg acactcctgc tggtttatcc ctttgtcagt ttgttcatct gtactgttgc
    24781 gggcttgctg gtaagaaaac tttcacagcg tgcattcagc gatctgttgt tctccccgcc
    24841 ttcactgccc gctgccgtca gttactctcg ttaaaccatc gccccagccg gggcgattca
    24901 atttgctaaa ccatgtcgat tacggataat gaacagcatt gcttatcaga gatatgcccc
    24961 catatgttga ggcatatcct aacgagaatc tgacaaccgt tatgcctgaa caatatcgtt
    25021 atacactgcc cgtcaaagcg ggtgagcagc gtctgctggg cgagttaacc ggcgcagcct
    25081 gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg tccggtggta ctcattgcac
    25141 cagatatgca aaatgctctg cgtttgcatg atgaaatcag ccagttcacc gatcagatgg
    25201 tgatgaatct ggcggactgg gaaactcttc cctacgacag tttttcgcct catcaggaca
    25261 ttatctcctc gcgcctttcc accctttacc agctaccgac gatgcagcgt ggcgtactga
    25321 ttgttccggt gaatacgctt atgcagcgcg tttgcccaca cagttttctc cacggtcatg
    25381 cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc attacgaacc caactggaca
    25441 gcgccggtta tcgccatgtt gaccaggtga tggagcacgg cgaatacgcc acgcgcggcg
    25501 cgttgctgga tctcttcccg atggggagtg agctgcctta tcgtcttgat ttctttgatg
    25561 atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca gcgcacgctg gaggaagtag
    25621 aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga taaagcggca attgaactgt
    25681 tccgcagcca gtggcgcgat accttcgaag tgaagcgcga tccggaacat atttaccagc
    25741 aagtgagtaa aggcacatta cctgccggga tcgagtactg gcagccattg ttcttcagcg
    25801 aaccactgcc gccgctgttc agttatttcc ctgccaatac cttgctcgtg aatactggcg
    25861 atctggaaac cagtgccgaa cgtttccagg ctgacacgct ggcgcgtttt gagaatcgcg
    25921 gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct ctggctgcgg gtggacgagc
    25981 tcttctcaga gctgaaaaac tggccccggg tgcagctaaa aactgaacat ttaccgacaa
    26041 aagccgcgaa tgccaattta ggtttccaga aactgccaga cctggccgtt caggcacaac
    26101 aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac tttcgacggt ccggtggtgt
    26161 tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga actgctcgca cgaattaaaa
    26221 ttgctccgca acgcattatg cgtcttgatg aagccagcga ccgtgggcgt tatctgatga
    26281 ttggcgctgc cgaacatggt tttgtcgata aggtgcgtaa tctggcgctg atctgcgaaa
    26341 gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga ttctcgccgc accatcaacc
    26401 ccgatacact gatccgtaac cttgcggagc tgcatattgg tcagccggtg gtccatctgg
    26461 agcacggtgt cgggcgctat gccggaatga ccacgctcga agcgggcggc attactggcg
    26521 agtatttgat gctcacctat gccaacgacg ccaaactgta tgttccggtg tcgtcactgc
    26581 atctgattag ccgttacgca ggtggcgcgg aagaaaacgc cccgctgcat aaacttggcg
    26641 gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa agtgcgtgat gtggcggcgg
    26701 aattgctgga tatctacgcg caacgcgccg ccaaagaggg cttcgcgttt aaacacgatc
    26761 gtgagcagta tcagttgttc tgcgacagct tcccgtttga aaccacgccg gatcaggcgc
    26821 aggccattaa tgcggtactt agcgacatgt gtcagccgct ggcaatggat cgtctggtgt
    26881 gcggcgatgt tggctttggt aaaacagaag tggcgatgcg cgccgctttc ctggcagtag
    26941 ataaccacaa gcaggtggcg gtgttggtgc ctaccaccct tctcgcgcag cagcattacg
    27001 acaacttccg cgaccgtttc gccaactggc cggtacgtat cgaaatgatc tcccgtttcc
    27061 gcagcgccaa agagcagacg caaatccttg cggaagtggc ggaagggaaa atcgatattc
    27121 tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt taaagattta ggcctgctga
    27181 ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga gcgcattaaa gcgatgcgcg
    27241 cgaacgtgga tattctgacg cttactgcaa cgccgatccc acgcacgctg aatatggcaa
    27301 tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc cgcccgtcgt ctggcagtta
    27361 aaacctttgt ccgtgagtat gacagcctgg tggtccggga ggcgatcctg cgtgaaattt
    27421 tgcgcggggg gcaggtttat tatctctaca atgatgtgga aaacatccag aaagctgccg
    27481 aacggctggc agaactggtg ccagaagcgc ggatcgccat cggtcacggg cagatgcgcg
    27541 agcgcgaact ggaacgggtg atgaatgatt tccatcatca acgtttcaac gtgctggttt
    27601 gtacaaccat tatcgaaacc gggatcgaca tcccgacagc caacaccatt atcattgaac
    27661 gcgcggatca cttcggtctg gcgcagctgc accagttacg cggtcgcgtc ggacgttcgc
    27721 atcatcaggc atatgcatgg ttgctgacac cgcatccaaa agcgatgact accgatgcac
    27781 aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg ggcaggtttt gcgctggcaa
    27841 cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg cgaagaacaa agcggctcaa
    27901 tggaaaccat cggtttctcg ctgtatatgg agttgctgga aaacgccgtc gatgcactga
    27961 aagccggacg cgagccgtcg ctggaagatc tcaccagcca gcaaacagaa gtcgagctgc
    28021 ggatgccgtc gctattgcca gatgatttca ttcctgacgt gaatacgcgt ttgtcgttct
    28081 ataaacgtat tgccagcgcc aaaacggaaa acgaactgga agagatcaaa gtcgagctta
    28141 tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct gctggatatt gcccgtctgc
    28201 gccagcaagc gcagaaactg gggatcagga agctggaagg taatgagaaa ggcggggtga
    28261 tcgaatttgc cgagaagaat cacgttaatc cggcctggtt gattggtttg ctgcaaaaac
    28321 agccgcagca ttaccgcctt gatggtccga cgcgcctgaa atttattcag gatttgagtg
    28381 agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg tgaactggaa gagaacgcga
    28441 tcgcttaatg gtaaatcggg ggcgtttctg cgcccccatc tgcaacattt acaaattctt
    28501 tgcacttccc tgcactatcc gacactgtca ccatccataa ttcaggctta tctgtttatt
    28561 acaataacct tatatttatt attgattttt ggtgatgatc aaaacgcgtt tttctcgctg
    28621 gctaacgttt tttacgttcg ccgctgccgt ggcgctaccg gcaaaagcca acacctagcc
    28681 gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa tttcatgtgg tggaaaatga
    28741 cggtggttct ctggaagcca tcgccaaaaa atataacgtc ggctttctcg ctctgttaca
    28801 ggctaacccc ggcgttgatc cttacgtacc gcgcgcgggc agcgtgttaa cgatcccgtt
    28861 gcaaacccta cttccagacg cgccgcgcga aggcattgtg atcaacattg cggagctgcg
    28921 tctctattac tacccgccgg gtaaaaattc ggtaaccgtg tatccaatag gtattggtca
    28981 gttaggtggt gacacgctga caccgacaat ggtgaccacc gtttcagaca aacgtgcaaa
    29041 cccaacctgg acgccaacgg caaacatccg cgcccgttat aaagcacagg gaattgagtt
    29101 gcctacggta gtgccggctg gaccggataa cccaatgggc catcatgcga ttcgtctggc
    29161 ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc gatttcggca ttggcatgcg
    29221 ggtaagttct ggctgtattc gtctgcggga tgacgatatc aaaacactct ttagccaggt
    29281 caccccaggc accaaagtga atatcatcaa cactccgata aaagtctctg ccgaaccaaa
    29341 cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag aagattgatg acgatccgca
    29401 gctgctgcca attacgctga atagcgcaat gcaatcattt aaagatgcag cacaaactga
    29461 cgctgaagtg atgcaacatg tgatggatgt ccgttccggg atgccggtgg atgtccgccg
    29521 tcatcaagtg agcccacaaa ctctgtaaat cctggataaa aaaaatcccc gcgacacggc
    29581 atgtcgcggg gaaaaaatca gcagtagcag gcattaatga gggttaatgc ttatttataa
    29641 attactgctg ttccatggag ggtattcgga ccggttacag aagtaatacg gaaagatttt
    29701 gcgcccatct catccgcttt ttgcgccagc tgctcttcca gcgatcccag atttgtcccc
    29761 gcgttagcac tgatagtacc gactttttgt tggccttctg gcgttgactg aacttcgaca
    29821 gccgcaaagt tggcaaatga catggagctt aaaatcgccg cagcgatgag ggtttttacg
    29881 tttttcataa tagtggcctt atgcagatga atgacgtcgc aagaaattac ttaacgatcg
    29941 ataactaaat gatagatgtg atctggatca catacaagat attttttata acaatcatta
    30001 attaatttaa aaatctttaa atttcagtta aatagaaagc aattattttt atgggatctg
    30061 gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc accaggttca
    30121 acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag
    30181 tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcaa cacggttatg
    30241 aagcgacttc tcttgcggac ctcgtcgaag cgaccggagc caaagcgccc acgctatacg
    30301 cggaatttac caacaaagag gggttatttc gtgccgttct cgaccgctat atcgatcgtt
    30361 ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg
    30421 ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg gcaggctgct
    30481 tcatgatcaa caactgcacc accctctccc cagattcagg agatatcgcc aatacgttga
    30541 aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc
    30601 gcggggaaat cccgacccac tgtgacgtga cacatctggc agaattcctt aattgtatta
    30661 ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg
    30721 ccggaacgac tttgcgttta tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc
    30781 agcctgagcg aatgaggggc caaattgcag agtacagcct tgttatactt tgttattcaa
    30841 aatcagttgc ccgttactat ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat
    30901 tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt tttctgactt
    30961 gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga
    31021 gccctggatc tggttaccgg catatccacc acccagcgcc cccacaacag tggcgacatc
    31081 tttaccacga ccaccaccaa actgatgccc tatcacgccg ccagcaacag cgccgagcac
    31141 cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg tgacgttgcg
    31201 acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa caacctgagc
    31261 gtattgcggg ccccgttcaa acacgttcag actggccact gccgctacgc ccagcgcagc
    31321 tgcgacacca atcccgatac ccgccaacat tgatttattc acgggaacac ctccttcttt
    31381 gcagttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga agatgaaaag
    31441 cgcgggaatg gctggaaata cttaaggatt cggagaagtc tgaaggaatg cctgatgcga
    31501 cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg gatgaggcgt
    31561 ttatgccaca tccgccagtg tacgtcgatt aatgcaactt caaacgcgga cggataacgc
    31621 ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc agcgcaatct
    31681 gatgcattcg gtatagcgag atatatacaa agcgcgcaat tcgtccttca atcatcattg
    31741 agccgcgcgt caggttaccc atcaggctac cgacagtgga gaagttcgac agcgatacca
    31801 gcgaaccatg atctttatac tgataatttt tcagcggttt accgttcatc tgcgccagaa
    31861 tatttttcat tgcgcaggtc gccatctggt gtgcagcctg agcacgcggc ggaacaaagc
    31921 ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg
    31981 tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca agaccaccga
    32041 tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga tcagcctcaa
    32101 tatattcacc atctttagtg tgcaggccgc cttcatcagc actggtgacc atggtttgcg
    32161 tcagcacgcg aacgccaagt ttcgttagct cgttgtgggc cgcagcagag atacgtggcg
    32221 gtaatgcagg caaaatacgt tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg
    32281 tcaggccttt gtaaccgtag ctgtgcagtt gcttgaccgc attgtacaat tcagcggaga
    32341 gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca
    32401 ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga cgcgcctggt
    32461 gcgggttatc gaggaaaatg cagttctctt tgacacctgg cgtattgaaa tcgttagagg
    32521 tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga accagcagtt
    32581 cacctttctc atcgcgcagt tctgcaatag tgattgtttt cgcttcacga tcaatatcaa
    32641 tgacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga tagctcaacg
    32701 catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt ttccacagat
    32761 ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc agcttatgcc
    32821 ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca atttttttca
    32881 atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta atggttataa
    32941 acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct attgagaata
    33001 acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttgt tgctgaaaag
    33061 ataggcgact ttaccgggtg agatcaaaaa aacagagttt attgtttatt atttgagcaa
    33121 gtccggaagg gtgatatagg gtgacgcgta tatcgagggc gtttggtcgt cctgataaga
    33181 tgcgcagcat cgcatcaggc attgatgctc gactgtcgga tgcggcgtaa acgccttatc
    33241 cgacttacag aattcggagc gcatcaggta ccggcaaggt tacctgatgc gacgagggca
    33301 tttacccgag ggttttgaac gccttaatgc gctgcaaatg cggggagata ttcttgaatt
    33361 tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg atgcaactct tcagatgtcc
    33421 gctggctgtt aagcgcttca tcattacgcg acaaaatcac caggcaacga tcgcgattct
    33481 tctcgcggaa gttggtcaca cacttagtgg caatatcggc atactcttcc gggcgatcaa
    33541 tcttgccttc catgttctca taagggaaca aattagggtt gaagatcacc tggcggatat
    33601 cgcaaagaaa accaatccgt tccgcccagt atccgcccag accaacgccg caaatcagcg
    33661 gacgctcgtc gacgttcagt tgcaacattt tgtccacttc tttgagcaga tgctgcatat
    33721 catgtttcgg atgccgcgta ctgtagctta tcaagcgtac atccgggtca ataaactgca
    33781 attgtaagac tttctcgtgg ttacccggac tgttagagtc aaaaccgtgt aaatagataa
    33841 tcatcgcttc ctcacataag ccagggttag tgacctgctt tctcttcctg ccagcgttca
    33901 tgtaactgat tcagacgggt gctgatcgcc ttccagcgag ccgagtccat cagttcctgt
    33961 cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt ctgccttgat cggtgacaga
    34021 ttatctaaca cgctgacggc ccctttacga ttattgcaga ccaggatcat atcgcaacct
    34081 gcgtccagtg atgcctgccc gcgttcggca taactgccca taatcgcggc accttccatc
    34141 gataaatcgt cagagaaaat tacaccgtca aaacacagtt cctgacgcaa aacggttttc
    34201 agccagtagg aagaaccgct cgccggacgc ggatcaacat cactgtagat cacatgcgca
    34261 ggcataatgg cgtcgagttt attttcgcgg attaaggaac tgaagaccga catatcttta
    34321 gcgcgaatct ccgcttgtgg acgtggatcg cacggtgttt ctttgtgtga gtctgccgtt
    34381 actgcaccgt gtcctgggaa gtgtttcccg gtcgttttca ttccggcttc atgcatacca
    34441 tcaataaacc ggctggcaat tgccagggct ttttgtggat cggcatgata agaacgctcg
    34501 ccaatcgccg cgctgatatg cccgacatcc agcactggcg caaagctgat atcaatatcc
    34561 atagcgatca tttcgctggc catcagccaa cccgcctctt gcgccagttt gccgccctct
    34621 tccattccca acagcgcagc aaaggattgt gctgccggta agcgggtaaa accttcgcga
    34681 aaacgctgca cgcgtccacc ttcctgatct accgccacca ccagatgatt gcgcgatgct
    34741 gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat catgatagtt acgcgtaaag
    34801 agaatcagcc ctcccaccag cggatgcgcc agtatttcac gctcttccgc gtccagttcg
    34861 taaccttcga catccaacat tactggaccc acactgctct ccttattgtt ttattaatag
    34921 ctgccgccag gtgtcatcgg ccagcctgat aaattgttga tcgccgattt gtcgccagcg
    34981 gtactcaaac caccctgctt tgagcatcag cagccaggga aaccatcgcc tgacctgacg
    35041 ccataattgc gccggataaa tcttcgcgcg agtggcatag tcattgacca attgccggtg
    35101 ctgttcagta ttttccaccc acaccgccgc cagttccagc gcgatatcac catctccggc
    35161 atactcccag tcgatgagtt ttaaccctga cgcgctatgc actaaatttc cggcgtggac
    35221 atccatatgc aatggactta agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt
    35281 taacattcgc agccaaccca cagtccgccg cgccggatcg ctttgctgcc agtacagttc
    35341 cagtaagggc aacagcgtta ttcgccagcc aaaacgtggt tgttgatgaa gataatacag
    35401 caagcctgcc agttcgttgg tatccggcaa atacgttttt accgcgccgg gcagatagtc
    35461 gactaccatc cagtcacgga gatataaatg cggcttcggt gcaatgcttg cgggtagttg
    35521 tgataaagcc cgatactggc gcaagaacgc ggactgcggc gcatcaggat cgtgcggctg
    35581 acgcacaaca aaacgctggc cctgatgttc aatgagaaaa ctaccgccac taagcccact
    35641 attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt
    35701 gatgggatta ttgctgcgaa acggcacctt tacctgacca gataatttcg cccgtctgca
    35761 ccagcatcag ctgcatttgt agggtcggag cgttaacgtt gccagaggcg ctggagtaca
    35821 gcacgtaatg agcgccgaca ttgcgggcaa tgcctatggc tttactacgg gtacctaaac
    35881 tgtcctgcgg cgacaaacct aactgttgct tagccatcga cagctgctgg gcggaaacca
    35941 gggtaaattt cccgttatta gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat
    36001 tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag caggacgcta cccgcagtca
    36061 ccccgtcagc cccaagcatc ttactgacca tcggctgcat tgcgccattc cagtcataat
    36121 ggcgaatatg cggcgcaggc ggtgccgttt gatcttcgtg ctcaattggg cctggctgct
    36181 gcgggatcgt cggcaccgag ggcactgtgg ggacaggctg ttgtggctcg gctggttgtt
    36241 ccggcgctgg tttcacttct tctaccggtg caggttcacg ttgccccaca cacccggcga
    36301 gaaacatcgc cagcgcggta atcaaggcgt agcgactcat ttttgtcatc aagattcacc
    36361 ccttacaaat atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac
    36421 agcgttaccg ccgaatgtgc gggaatgcta acgctgcgtg gcctttccag aggatgcatc
    36481 tccagccctc tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt
    36541 tcgttataaa gcgttgagga tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt
    36601 tctgcactga tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc
    36661 accggaattt ccggatgtga acgacagccc accagcagta acaacgccag agacaccagc
    36721 ccaaagcatc cttttctcat cgttacagac ctttatgcgc cagcattggt cccagcggac
    36781 ggccacccaa caagtgcata tggatgtggt aaacctcttg tccgccatgg cggttggtat
    36841 tcatgatcag acgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta
    36901 cggtgatcat gcgtcccagc gcctgctcat gctcagctga cacgtcgttc acagtcggaa
    36961 tgaggatatt cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg
    37021 ccgttaccag atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc
    37081 tgaatatagt ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat
    37141 gctacgccgc gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc
    37201 ttaactcacg atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc
    37261 catttctgcc tgcgaatgcg ctgaaaagac attctctgtt tggtttacac gacaaatatg
    37321 aatgcgcata tttctcattt gcatttacaa acaaaattat tcgcacacta aatataaccg
    37381 ctcatcaccc tcctccggca tgagcctgac gacaacataa accaagagat ttcaaatgct
    37441 ttcaacacaa tttaacaggg ataatcaata tcaagccatc accaaaccgt cactacttgc
    37501 cggttgcata gcactggcac tattaccttc tgccgctttt gctgcaccag ccactgaaga
    37561 aacggtgatt gttgagggtt cagccacagc tccagatgat ggcgaaaatg attacagcgt
    37621 aacgtctacc tctgcgggta ccaaaatgca gatgactcaa cttgatattc ctcagtcggt
    37681 cactattgtt agccagcagc ggatggaaga tcagcagtta caaacgctgg gcgaagtgat
    37741 ggaaaacacg ctggggatca gcaaaagtca ggcggattcc gatcgtgctc tttattattc
    37801 ccgtggattc cagatcgata actatatggt tgatggtatc cccacctatt ttgaatcgcg
    37861 ctggaatctg ggcgacgcac tttctgatat ggcactcttt gaacgcgtag aagtagtgcg
    37921 tggcgcgaca ggactcatga ccgggacggg taatccatct gcggcaatta atatggttcg
    37981 aaaacacgcg accagtcgtg aatttaaagg cgatgtctcg gcggaatacg gtagctggaa
    38041 caaagaacgg tatgtggcgg atttacaaag cccactcacc gaagacggta aaatccgcgc
    38101 gcgaattgtc ggcggctacc agaataacga ctcatggctg gaccgctaca acagtgaaaa
    38161 gaccttcttc tcgggcattg tcgatgctga tttaggcgat cttacgacgc tttcagccgg
    38221 ttacgaatat cagcgcattg atgttaatag cccaacctgg ggcggtttac cgcgctggaa
    38281 tactgatggc agcagcaaca gttacgatcg tgcacgcagt accgcacctg actgggcgta
    38341 caacgataaa gagatcaaca aggtctttat gaccctgaag cagcggtttg ctgataccta
    38401 gcaagcgaca ctgaatgcca cccactctga agtcgaattt gacagcaaaa tgatgtatgt
    38461 cgatgcctat gtaaacaaag cggatggtat gctggttggg ccatacagta attatggacc
    38521 tggctttgat tatgtcggcg gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct
    38581 ggatttgttc gctgacggta gttatgaatt gtttggtcgt cagcacaatc taatgtttgg
    38641 tggcagttac agcaaacaaa acaatcgtta cttcagttca tgggccaaca tcttcccgga
    38701 tgaaattggc agtttctaca actttaatgg caatttccca caaaccgact ggtcaccaca
    38761 gagcctggca caggacgata ccacacatat gaaatcgtta tatgctgcta ctcgagtcac
    38821 ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat accaactggc gggttgatac
    38881 gctgacttac agcatggaga aaaaccacac cacgccttac gctggtctgg tgtttgacat
    38941 caatgacaac tggtcgacct acgccagcta tacctctatt ttccagccgc aaaatgatcg
    39001 tgacagttca ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa
    39061 atcggactgg atgaatagcc gtctgaccac cacgttagcc atcttccgta ttgagcagga
    39121 taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa
    39181 agcggtggat gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg caattaccga
    39241 caactggcag ctgacatttg gcgcaacgcg ctatattgca gaggataacg aaggaaacgc
    39301 cgttaatcct aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt
    39361 catgccagag ttgacagtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac
    39421 cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt
    39481 cacccgctac caggtgacga aaaacttctc gttacagggg aacgtcaata acctgttcga
    39541 caaaacctac gataccaaca tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag
    39601 cattaccggc acgtatcaat tctgaataac acctgtaaaa aaggcagcca tctggcggcc
    39661 ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt
    39721 caggttatcg gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc
    39781 cgctgcgccc agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac
    39841 gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc
    39901 aaccagagcc ggtgccattt cgctggtacc tgttgctttt gcatcttcag tcgcgtcttc
    39961 acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac
    40021 aatcgcataa ccgataccga cgatcgggaa cagccacagt ttgctgctgt taccagacag
    40081 aacgataaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat
    40141 acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac
    40201 gaacatgaag gagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc
    40261 ggagatcata ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc
    40321 aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc
    40381 agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt
    40441 ggtgtattca ccaatctgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg
    40501 caccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc
    40561 agcccactga gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac
    40621 aacgccagta aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa
    40681 gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat
    40741 cgcaccggag ataatccctc cgagtacgcc agtatccgcc aggtgtttag aggcgatttc
    40801 ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc
    40861 ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac
    40921 accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac
    40981 atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc
    41041 tgcgataggc agtacggata ccggcagcat cagcgattta ccgacctttt gcaggttagc
    41101 aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct
    41161 cacgcgtggc aaggggggag agcctcgccg tgtacagggc atctaagcgc cctttattta
    41221 ttacacagag taaaataatt cagtgccgat ttgtttgacg gctatcacat ttcaacttca
    41281 gatcattgga gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgtggtt
    41341 attcaccgcc caattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt
    41401 aaaaaaactc attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc
    41461 gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat
    41521 gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg
    41581 cgcaaggtaa ggtgagtcag tttccaccag taaccgatcc agggggacat aacgcgcagc
    41641 atctcgaagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc
    41701 gagatccagt aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc
    41761 gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt
    41821 atggacgata accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc
    41881 ttgctgacga actttagttt ccggtgtgta ataataatcc agcccggttt cacccagcgc
    41941 tacaacaccc tcttctgccg ccagacggcg taaatcttct acatcgtagg gatcattctg
    42001 gttgagtgga tgcacgccac aagaaaatac gacgttgtca cgttcgccta ccagatcccg
    42061 catatgtaaa taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc
    42121 ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc
    42181 gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta
    42241 gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct
    42301 cgcggttgat gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta
    42361 tagcctgcag gcgcgaggga gaaagatggt ttgccagttc gacgacaagg cccggcacat
    42421 caacattggt cacctgcgca gcaccatgat ggcgttttag cgcatccatc agcaacgttg
    42481 ccagccagtg taaacgcgcc ggagcttgtt catgattaag ggccgccagc agcgaatacc
    42541 agtcgcccga tggcacgcta tatgccaatg cctgacacaa tgtttcacga gcctgccagt
    42601 tatctccctg aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg
    42661 caagtaatgc atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt
    42721 ccggcggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt
    42781 caggctcgcg ggtagccagg aaaaaccagg tttctgctgg tggctcttca agcgttttca
    42841 gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc ggttacccag acaactttcg
    42901 caccacctag gcgtgcgtgc tcattcagct tttcggtgac ctcacgtact gcatcaatgc
    42961 ccagcgtatt tttccctttt tcgggagcca gggtgtagta atcgggatgc gtgccagcct
    43021 gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga
    43081 gtaaataacg gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa
    43141 tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag
    43201 gtcgtaacca tggataccat ctcatgcgtc caactccttc acccagtggg tcacggtagt
    43261 gcggattgca tccatcacgg cctccagcgg ctgggtggca tcaatggtat gaatactttt
    43321 atcttgtgca gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc
    43381 ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt
    43441 aacatcgaga tagagcgtta agtcggggcg aaaatccccg agaacagcat cacgcagtgt
    43501 tgccagcata tgttggtcaa taccacgtcc tccgccctga tacgcctgag tggagagatc
    43561 gtggcgatcg ccaatcaccc aggtgccgtt agccagagct ggtttgatga ccgtttctac
    43621 cagttgaacg cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc
    43681 gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc
    43741 aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcaagcgtct caaccaccac
    43801 attacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg
    43861 catttttttc cttaagcact ttcagataat cctgcacaga cttgttatgg ctggcaagat
    43921 tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg
    43981 ccggatgcgc agcagccttc agcgaatccg cccccggcgt agctatcgca cccggcggca
    44041 aaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt
    44101 tgccattata acgctctccc atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac
    44161 caatgcgtaa acggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg
    44221 tttctttttc gataattgat gccatcgtca ccagctgatt tttatcttta taaggcagac
    44281 cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg tgcgctcgct
    44341 tgagtaacgc gacatcggtg gtattggcgg tatacatcca ggtgtctggc cagaaccaac
    44401 cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt
    44461 cactcagcgt gtgcttgata tacggagcct cacgcaattg cttgaggtag tcgctcagac
    44521 gcatcccttc taccagtcgc agcgggaact gtgcttcttt accgctttcc agcaatttca
    44581 gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa
    44641 gatccggttc gatacgcagc aaccattgaa aaacccgtgg gcgattgatg atcttatcgg
    44701 cataaagctg ttcaccaagc gccagacgtc cggtccctgg cttcagggta aatatcgtct
    44761 cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag
    44821 cggcgatacc cagtaccacc agcaataaca agataatcaa taacactttt ttcatgacta
    44881 attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggagac
    44941 atcgccacag gcacgtacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc
    45001 tgcctgcaac gcctcttcca gagaggcttg cacttcgaca agctgataag aggattgtgc
    45061 cagcaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagacgcgg
    45121 cgtataaact acattgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca
    45181 cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcggat
    45241 caatacttgc tcaagccgat taagatgttt aatacctgca agatgaggat tgcgccccag
    45301 ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg
    45361 ataaaccgta acggagagaa tccgcgttgc cggtccactg ttcaatgtgc tgtaccctcg
    45421 cccgccacta ccgcgactga tcacgacttt cagcacacca ttttgctgtt ctgctgccag
    45481 cgttttcatc tcttgttcaa gctgaggcca gaagtcacag aaaatcatca accgctgaca
    45541 agcatcctgt agtcgctgga tatgcgccga taacaaactg actttaccgt cgataactct
    45601 ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc
    45661 ctgcttatga ccgttaatta agaacatacc ggctccttat cagagaattg ttagtgtggc
    45721 agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac
    45781 cttttatatg ggtgggaaat gacaacttag atctttttaa agatcaaaga accattagtg
    45841 ccaccgaagc cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttca
    45901 tgcggtacga aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga
    45961 acagcctgat cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct
    46021 aacaggtgac cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg
    46081 aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc
    46141 gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcagagc atttgccatc
    46201 gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc
    46261 ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac
    46321 tcttcaagta ccagcatacc ggcaccatcg cccagtacga agccatcacg ctctttatcc
    46381 cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca
    46441 aaaccaccaa cacccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca
    46501 gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag
    46561 gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc
    46621 accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc
    46681 atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg
    46741 gcaccaatgc gggttgcgtt ctcttccgtt atatcaaggc cagaatcctg catggcctga
    46801 acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag
    46861 ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta
    46921 gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta
    46981 gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc
    47041 ttagacacgt ttgtcctcca gggagggaaa aaatgattct agtgggacaa aaagataaaa
    47101 ctcaggcggt cgaacgaccg cctggagatg ttcacttacg cctggtggcc gttgatgtaa
    47161 tcaatggcag cctgaacggt ggtgattttc tcagcttctt cgtccggaat ctcagtatca
    47221 aactcttctt ccagagccat taccagctca acggtgtcaa gagaatccgc gcccaggtct
    47281 tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa cgcccagctg ttcgccgata
    47341 attttcttaa cgcgttcttc gatagtgctc atactcttaa atttcctatc aaaactcgct
    47401 ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg
    47461 tcttaccacg attttacgtt attttgaggc cttacccccc aataacgcaa atcattttgc
    47521 actaattatt actcataacc acgcagactg cgcgcaacat gagcattttg tgcaaatcgc
    47581 ggtcagacca tgtacatccc gccgttcaca tgcaaagttt cacccgtgat gtaagctgct
    47641 tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc
    47701 gcaggaacct gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc
    47761 gtttcaataa agcccggagc aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc
    47821 gccagtgatt tactgaagcc gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg
    47881 ccatttccca tggtaccaac cacagaaccg atagtgataa tacgaccatg acgctttttc
    47941 atcatagcgc gcattaccgc ttttgacaga cggaaaacag atgaaaggtt ggtttcgata
    48001 atatcgttcc actcttcatc tttcattcgc attaacaggt tatcacgagt gataccggca
    48061 ttattgacca ggatatccac ttcaccaaat tctgcgcgaa ttttttccag aacagattcg
    48121 atagatgccg ggtcggtcac attcaacatc agacctttgc cgttggcacc taaataatcg
    48181 ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa taactttcgc gccacgggct
    48241 gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg caccggttac cagtgcgatt
    48301 tttccttcaa aattcatgat tttcctcttt taaagctcaa gcgccgctgc catcgctgaa
    48361 ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc
    48421 accttgcccg ggccgacttc atagagatgt tctacgcctt gcgctgccat gtactcaaca
    48481 gacttcgtcc actgaaccgg gttatacaac tgacgtacca gtgcgtcacg gatggcatca
    48541 ccattggttt cgcatttcac atcaacgtta ttcacaacag aaactgttgg tgcgttaaag
    48601 gtgattttcg ctaattctac tgccagtttg tcggctgctg gtttcatcag cgcacagtga
    48661 gacggtacgc tcactggtaa cggcagagca cgttttgcgc ccgcagcttt acaggcagcg
    48721 ccagcacgct caaccgcttc tttatgaccg gcaataacca cctgtcccgg agagttaaag
    48781 tttaccggag aaacgacctg acctgctgca gcttcttcac acgctttcgc aatagacgca
    48841 tcatccagac cgatgattgc cgccatagcg cccgtacctt ccggtacggc ttcttgcatg
    48901 agcttgccgc gcatctcaac cagacgcacc gcatcagcga aatcaatcac accagcgcaa
    48961 accagcgcgg aatattcccc caggctgtga ccggccatca ttgccggtgc tttaccgccc
    49021 tgctgctgcc atacgcggta cagcgcaaca gaggcagtca acagcgctgg ctgggtttgc
    49081 caggttttat tcagttcttc agctggcccc tgctgggtca gcgcccacag gtcgtagccc
    49141 agcgccgcag aagcttcagc aaacgtttct tcgacaattg gatagctcgc cgccatatca
    49201 gccagcattc caacggtttg agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg
    49261 ttttaatcct tatcctagaa acgaaccagc gcggagcccc aggtgaatcc accgccaaag
    49321 gcttcaagca gaaccaactg ccccggctta atgcgcccgt cgcgtacagc ttcatccagc
    49381 gcgcacggga cagaggccgc agaggtatta ccgtggcgat ccagcgtcac cacgacattg
    49441 tccatagaca taccgagttt tttcgccgtt gcactgataa tacgcaggtt agcctgatgc
    49501 ggaaccagcc agtccagttg agaacggtca agattatttg ccgccagcgt ctcatcaacg
    49561 atgtgcgcca gttccgttac cgcaaccttg aagacttcgt tgcccgccat cgtcagatga
    49621 attgaattct ctggattcac acggtcagca ttaggcagcg tcagcaactc accatagcta
    49681 ccgtcggcat gcagatgggt ggagatgatt cccggctctt cagaggcagc cagcaccgca
    49741 gcgcccgcgc catcgccaaa aataataata gtcccacgat cggttggatc gcaggtgcgc
    49801 gccagtacat cggaaccgac gaccagagca tacttcaccg ccccagattt cacgtattga
    49861 tcggctacgc ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg
    49921 caacctttaa tgcccagcat gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta
    49981 gcagaagtcg ttgccacaac gatcaggcca atctggtctt tctcaatgcc cgccatctca
    50041 attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg
    50101 tggcgttcgc ggataccggt acgagtgaca atccactcgt cagaggtgtc caccattttt
    50161 tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc tgccagtacc aataatcttc
    50221 gtatacatgt acgctcagtc acttttcggt tatataccgt cacttgcaaa ctgcgagttc
    50281 gctggcagcg tcctgctacc gcagagttcc gcttttgcca ccgtccagca gctcaaaacc
    50341 agctgggtat acagattcca ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc
    50401 ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac catgactttt
    50461 tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg
    50521 actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt aataacagta gccaccacga
    50581 ccgttttttc ccttcaccct gagatttcag cagagaaagg aacatcctga caacaccttc
    50641 catcgtcttt aatgtgacat ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc
    50701 agttaacaac tcattggctt caagatagcc gatataattg atagaaggga ttgttttaag
    50761 caccgctgag gcatcccgaa tactgtcgag accctttact tcttcttcac caatattgag
    50821 caacgccacg cgaggattgg gaatttccac cacctcttca gccagaactg agcccataat
    50881 ggcaaattgc accaacattg tactgtcaca atcgacgttg gcccctaagt caaggaccac
    50941 cgttttgccc ttttgctgat gtggtaatac cgtcaccagc gccggacgct caatcccctc
    51001 caggggcttg agtaataatt ttgccagccc catcagtgcc ccggtattac cggcactgac
    51061 acaggcttgc gctcgacctt ctttcaccag ctccagggcc acgcgcattg aactcccacg
    51121 actggcgcgg atagcttgcg aaggccgggc atcactggcg ataactgact gcgcaggaat
    51181 aatctgcaga cgcgaacgtt gttcaaagtc agctttagca agtaatggcg tgatggcgtc
    51241 gggattgccg actaaaagaa gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc
    51301 aggcactgtc acggaagggc caaaatcccc tcccatgaca tctaacgcca gggttagacg
    51361 tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg aaaatcctca
    51421 ctaagcttca tcacgcagac gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa
    51481 ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc tacagacagg
    51541 ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga acgggttggt
    51601 ttattctgtt gtacggccat ggaccttact cctcaattac ggtttatccc cgatccaacg
    51661 gggagcacca attacttacg ctttaagctg gctaatacgg caaatgggtt tggcttttgc
    51721 acttcttcag gcagttcacc aaagaccatg tccgcttcgg acacttcaca gtgttcagaa
    51781 tcatgcaccg gaactaccgg caaggcgagg atgatttcat cttcaaccat tgcaagcaga
    51841 tcgatttcac cgaattcgtt aacctcaatc ggttcatacg cttccggcag tgcttcagcc
    51901 tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt agacctgatg agtaaacggc
    51961 ttcccgcaac gctgacactc gagcgttacc gtcaccttcg catcgccgtt taacactgcg
    52021 agacgttggt tatcgatagc gaacgacatg gagcattcca catcactgtc cacactgact
    52081 acggattcgg cgacgcgctc aacctgatca ggggtataga taccctggta atcaaggcgt
    52141 ttttgagccg tacgaaccgg atcgagagtc aggggtaatt ttaccttttg catagggcgc
    52201 gcatattaac tttgtaacgt catagagtca aagaaaaagg cagcctgtgg ttgccttttg
    52261 ccaataattc gcacacattg cgggttactg ctttattttc gttcagtgct gataacgaac
    52321 atcaacctgc aaaacgaagc gcatagttta aaatggctat catcaatacg ctatatctga
    52381 tggaaaaaaa tatgcctaaa cttattttag cctccacctc gccatggcgt cgcgctcttc
    52441 tggaaaaact gcaaatctct tttgaatgtg cagcaccaga ggtcgacgaa accccacgca
    52501 gcgacgaatc accgcgacag ttggtgcttc gactggcaca agaaaaagcg caatctctgg
    52561 cgtcacgtta tccggatcat ttaattattg gttcagacca ggtatgtgtt cttgatggcg
    52621 aaatcaccgg taaaccgtta acggaagaaa atgcccgtct gcaattacgc aaagccagcg
    52681 gcaatatcgt caccttctat actggactgg cgttgtttaa ttcggcgaat gggcatctac
    52741 aaacagaagt ggagcctttt gacgtccatt tccgtcacct gagcgaggcg gagattgata
    52801 attacgtgcg taaggagcat cccctgcact gcgcgggtag ctttaagagt gaaggatttg
    52861 gcattacgct gtttgagcgt ttagagggac gtgaccctaa cacgctggtt ggtttaccgc
    52921 taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa cccgctgatg ggatagttgt
    52981 ttgcgctgga tgcggttacg tcgcatccgg caatcggtgc aggcggcctg atgcggcgta
    53041 aacgccttat caggccaacc gcccattatc aggcttatat gcttgtatta gcgcgcgtta
    53101 cgcagctttt gcaaacaacg cttcaaacct tcatccatcg gcgcttcgat acgcatcacc
    53161 tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt gcaggaacag acgatttaat
    53221 cccgtgcctg cttcagtgag ctgtctgtca aattcacggt caccgtagcg atcgtcaaag
    53281 gcaatcggat gacccgcata ctgtgtatgc acacggatct gatgagtacg tccggttacc
    53341 gggctacaac gcaccagggt ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc
    53401 gacggtttgc cttcctgact gacacgcacg atacgttcgc cgctttgcag aatatttttc
    53461 aataacggcg cttgaacgct cttcacatgc gactgccact gaccgcgcac cagcgccagg
    53521 taatcttttt gcattccttt ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt
    53581 tttttcgcta tcagcaacac acctgaagtg tcccggtcga gacgatgaac cagttcaagg
    53641 aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa cgccgaagct taaaccactg
    53701 ccgccatgta ccgccgtacc ggaaggttta ttcagcacca ggatatgatc atcttcatat
    53761 aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc
    53821 cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag tttatattca
    53881 ggcttaatac gttttttgtt cacccgcact tcgcctttac gcaaaatacg gtaaatcata
    53941 ctttttggta cgcctttcaa ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg
    54001 tcagcggtga tagcaacaat ttttacggat ggagtctctg ttttcatggt gggcgattct
    54061 aattagccaa caggattcgc gccactcatt tttctatgct tatatttagt tttgcacctt
    54121 attacttcac tacgtgatca ctttattgat ggttattaaa ccaatcacca gcaagaagtg
    54181 aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca tcttgctata acaaggcttg
    54241 cagtggaata atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa
    54301 taacccattt tgcccgaccg atcatccacg cagcaatggc gtaagacgta ttgatctttc
    54361 aggcagttag cgggctgcgg gttgcagtcc ttaccggtag atggaaatat ttctggagag
    54421 taatacccag tctgtttctt tgataattgc gctgtttttc cgcatgaaaa acgggcaacc
    54481 gacactctgc gcctctttga gctgacgata accgtgaggt tggcgacgcg actagacacg
    54541 aggccatcgg ttcacacccg gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa
    54601 gaataatgag taagttacga tgaaaagaat gttaatcaac gcaactcagc aggaagagtt
    54661 gcgcgttgcc cttgtagatg ggcagcgtct ttatgacctg gatatcgaaa gtccagggca
    54721 cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc cgcattgaac cgagtctgga
    54781 agctgctttt gttgattacg gcgctgaacg tcacggtttc ctcccactaa aagaaattgc
    54841 ccgcgaatat ttccctgcta actacagtgc tcatggtcgt cccaacatta aagatgtgtt
    54901 gcgtgaaggt caggaagtca ttgttcagat cgataaagaa gagcgcggca acaaaggcgc
    54961 ggcattaacc acctttatca gtctggcggg tagctatctg gttctgatgc caaataaccc
    55021 gcgcgcaggt ggcatttctc gccgtatcga aggcgatgac cgtaccgaat taaaagaagc
    55081 actggcaagc cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg ctggcgtagg
    55141 caaatctgct gaggcgctgc aatgggattt aagcttccgc ctgaaacact gggaagccat
    55201 caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt catcaggaga gcaacgtaat
    55261 cgttcgcgca ttccgcgatt acttacgtca ggacatcggc gaaatcctta tcgataaccc
    55321 gaaagtgctc gaactggcac gtcagcatat cgctgcatta ggtcgcccgg atttcagcag
    55381 caaaatcaaa ctgtataccg gcgagatccc gctgttcagc cactaccaga ttgagtcgca
    55441 gatcgagtcc gccttccagc gtgaagtccg tctgccgtcc ggtggttcga ttgttatcga
    55501 cagcaccgaa gcgttaacgg ccatcgacat caactccgca cgcgcgaccc gcggcggcga
    55561 tatcgaagaa accgcgttta acaccaacct cgaagctgcc gatgagattg ctcgtcagct
    55621 gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc atcgacatga cgcctgtacg
    55681 ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat
    55741 tcaaatcagc catatttctc gctttggcct gctggaaatg tcccgccagc gcctgagccc
    55801 atcactgggt gaatccagcc atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg
    55861 tgacaacgaa tcgctgtcgc tctctattct gcgtttgatc gaagaagaag cgctgaaaga
    55921 gaacacccag gaagttcacg ccattgttcc tgtgccaatc gcttcttatc tgctgaatga
    55981 aaaacgttct gcggtgaatg cgattgaaac gcgtcaggac ggtgttcgct gcgtgattgt
    56041 gccaaacgat cagatggaaa ccccgcacta ccacgtgttg cgcgtgcgca aaggggaaga
    56101 aacgccgacc ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc
    56161 tgaagaagag ttcgctgaac gtaagcgtcc tgaacaacct gcactggcaa cctttgccat
    56221 gccggatgtg ccgccagcgc caaccccagc tgaacctgcc gcgactgtcg tagccccagc
    56281 accgaaagct gcaacggcaa caccagcagc tcctgcacaa cctgggctgt taagccgctt
    56341 cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa gccaaaccga ccgagcaacc
    56401 aacaccgaaa gcagaagcga aaccggaacg tcaacaggat cgtcgcaaac ctcgtcagag
    56461 caaccgccgt gaccgtaatg agcgccgcga cacccgtagt gaacgtactg aaggcagcga
    56521 taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg
    56581 tgagagccgt cagcaggctg aggtaacgga aaaagcacgt accaccgacg agcagcaagc
    56641 gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc
    56701 gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg
    56761 tccggttcag ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag
    56821 cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa actgccgctg ccgaaccaat
    56881 tgttcaggaa gctccagctc cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc
    56941 gcaaactgca ccagaacagc aagaagagaa caatgctgat aaccgtgaca acggtggcat
    57001 gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg
    57061 ctatcgtgac gagcgttatc caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc
    57121 tccggaactg gcctctggca aagtctggat ccgctatcca attgtacgtc cgcaagatgt
    57181 acaagttgaa gagcagcgcg aacaggaaga agtacaagtg cagccgatgg tgactggggt
    57241 ccctgtcgcc gccgctgtcg aacctgttgt tagcgcaccg gttgttgaag aagtggctga
    57301 agttgtagaa gcccccgttc agattgccga accgcaaccg gaagtggttg aaacgacgca
    57361 tcctgaagtg attgccgccg cagtaactga acagccgcag gtgattaccg agtctgatgt
    57421 tgccgtagcc caggaagttg cagaacacgc agaaccggtg gttgaaccgc aggaagagac
    57481 ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt
    57541 tgctcaacct gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa cggtagctgc
    57601 ggttgaacct gaggtcaccg ttgagcataa ccacgctacg gtgccgatga cgcgcgctcc
    57661 ggcaccggaa tatgttccgg atgcaccgcg tcacagtgac tggcagcgcc ctacttttgc
    57721 cttcgaaggt aaaggtgccg caggtggtca tacggcaaca catcatgcct ctgccgctcc
    57781 tgcgcgtccg caacctgttg agtaataatt agctcaagta atcaagccct ggtaactgcc
    57841 agggcttttt tatttcatct ttgaatcaac atattaaaat tcatcgaatg gcatccttgc
    57901 taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg gcgatcccag agcaggcaga
    57961 caaaacatac ccagtttcat gatatgtttt aaagcgaaat tatttgttga gctggaagag
    58021 cgacaatccc tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg tttgctgcat
    58081 gatgtaagat gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt
    58141 ttgccctaaa gcgcgatcgc tacctaatga atccagcgac tccagttcgt tcagctgcgt
    58201 gcctaattcc gcgcgaacgg tcagcacatt gttcagcgag tttttcagtc cgcggttggt
    58261 tttatctaac gccgcagcgg cggtttcttt atccgcttcg ctatccgcga ccggcgtttt
    58321 cagggctgcg atggcgctat ccagcatggc aaaaagattg gtttcagaag cgctaccgtc
    58381 tggttccgct accgcgttac tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat
    58441 caccatcgaa cgcgaagcat cgacctgttg cttaatactt tctgttccgc cgacataatc
    58501 accgttagcc tcactaaacg gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc
    58561 attaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct gaatatccgt
    58621 agccagcgag gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga caattttttc
    58681 ctgagcattc tggatagcag tggtgacctg gctaagtaca ctctcttcca gtgacacttt
    58741 ttgagtggcg aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag
    58801 aacaaccgct tgtgatgcag caatgggatc atctgaaggg ttaacgactc gcttacccgt
    58861 cgacatctgt tcgccgtact tcatccattc tgcctgagaa ttggtgatac cacgcatgtt
    58921 ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg aatgttaatc
    58981 agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc
    59041 tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc
    59101 tgattggaaa gctgcgtcac cacattacct tgcgtggtgc tactggtttt caacgtcgcg
    59161 gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga tttcgcaccg
    59221 cccaccgttt tactattgct ttgcagatcc agcagggcct gaccgttgcg gttatcgcta
    59281 tcacccgcat ctttttcgct cgccatcgct attttcgctt cgtcggtgat taatacatcc
    59341 atgttgacga tggcgtcact tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt
    59401 cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc cggcgtcacc
    59461 gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc gaacgagatt
    59521 ttgtaatctg tcgccagtac cacggaggca tcagttaccg tggcaccgat cgcaacgtca
    59581 cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga tagtaaagaa atcttcacca
    59641 gcatcgccgt tagcatcaaa tccggctttg tgttgggtgt tgaaagcctc ggcaaatgcc
    59701 agcgccagtt gtccaagcgt attacgcgtc tggtccagct cctgagaacg gaatgtcaga
    59761 atgccgccca gcgacccggt attcagtaat ttctccggga tctcaatatt gcctgccgtc
    59821 ccatcaacat aagcgacagt cgtacgagaa gggtcagcgc tggaaggaac tgccgccagt
    59881 tgccgcgccg tacttccctg aaccagtgag taaccattgg ccaccgtgat gttataagtg
    59941 ccgccatcct gaacgctgac ttctacacca acaatctggt ttaattcgct caccagttga
    60001 tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt caggcgcgag
    60061 atttgatcgt tcaggctggc aatttgttta gcgtaattgt tgatctgatc aacgctggca
    60121 cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt ggttttaaac
    60181 tgattcacca acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg
    60241 ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag
    60301 gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc ggtcaggccg
    60361 ctactttgcg tctgcgcagc acgtaactgg ttggtaataa acgcatcata ctcacgctgc
    60421 acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt gctattggcc
    60481 tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta
    60541 cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag
    60601 ctggacatgg aggttcctta taagccttca attacagagt attatcggca gcgacccgcc
    60661 ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg ctcaccttgt
    60721 cgcttatcga tttcatctgc tggatcatgt tggtgagttt acgggcatag tgaggatcgg
    60781 tagcataccc cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg gctgtcgtca
    60841 cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta atccgacaat gcttccagat
    60901 acgagctgta gacccgaaat ttcgctttta ctttcttcgc ttcgccattt tcatattcag
    60961 tcgtggtgat ttcagtaact ggacctttcc agttgccaga ggctttgacg ccaaacaggt
    61021 tatagctcgg ctcgccgttt tcgcggcgga tttgccgttg cccccaacca gactccagcg
    61081 ccgcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg
    61141 gcagcgagag ttgcgcgagg aatgctttac tgtcacccgg cagcgaatca tcgtagttac
    61201 gtggcacggc cttttgcacc agctgcgaaa gcgcctgatt ttgataacgc accacagttt
    61261 cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg
    61321 tcatctgttt aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc atctgttggg
    61381 caatctgctg gtcatacata ctggtataca ggcgagtgtg ctcgctgctg aacaggccat
    61441 cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct
    61501 gacgggccac cggacggata tttgccgccg gatcttcgcc cgctttcgcc tttagttcgt
    61561 tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat
    61621 gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcagta tggacatcag
    61681 atccatcggc gtagcgccca gcgcattgag cgcgcgcacc acgttattga ggctggcgct
    61741 ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt
    61801 aaccacagtc tgtccaccac caaacggtgt atctggctgg ctgacattgg cctgacgatt
    61861 gactgttact gagagatttc cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt
    61921 catcaccacc gaaccggtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt
    61981 aacctgcata ttctggatat ctgcaaggaa acggacctgg gaactgttgc cacttggtac
    62041 gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac gcacgcggtt
    62101 gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag
    62161 ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac cattggtgat
    62221 ccgtccaccg ttcagttggt tcacctgaac actgctaccg ccagcggagg ctcctgcgcc
    62281 gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg
    62341 cgtcatcaac aacgtacctc cacgcaagct tttggcattt cccatggaag aaaccaccac
    62401 atcgatggtt tgcccctgac gtccaaacgg aggaagtgac tctgtcacca ttaccgcagc
    62461 gacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat
    62521 gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg tgccatccag
    62581 ccccaccacc agaccatagc caatcagtga gttttgcctt accccctgaa cactggtgag
    62641 atcgcgaata cgctcagcct gagccgccgt cgtaaccagt agaagaatta atgcagagag
    62701 aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac
    62761 cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat gcgcgcatcc
    62821 gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgcca
    62881 gagaagcgaa taaattcggt accctgatta atggcaatct gtttttcacc caccacatgc
    62941 aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg
    63001 ctggcattgg ccccgccctt tccgttgaac gtgttaccac cggaggcttc gacatcggca
    63061 cgagcgttac caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt
    63121 ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac
    63181 acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag
    63241 ccatagttaa tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcggaccg
    63301 ggaaccggtt gtgcactggt cgccccctgc accagcggcg tggagggtat ccaggcgcag
    63361 ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc
    63421 atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta agccttagag
    63481 ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta
    63541 agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc
    63601 aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtac
    63661 accagaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt
    63721 cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc cttgttgggt
    63781 tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat
    63841 cgctggctgc acctgaaaac caccagctgc ccgttctgat ccacctggaa agagccgtca
    63901 cgggtatagg ctgatgaacc atctggcaac atcacctgga aaaagccctg ccctttaatc
    63961 gcgacatctt tgctgttgtt ggtctgcgac aggtttccct ggctgtgtaa gcgttcagtg
    64021 gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa
    64081 gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga
    64141 cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt
    64201 tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta
    64261 gtggattagt gacaaagtgc atatgtcttc ttgccggatg cggcgtaaac gccttatccg
    64321 gcctacaagt gctcgcaaaa tcgataaatt gcgattgccc ccatagaccc gataaacttg
    64381 cgcatcgggc aacttatact tgtcatgtat cctttcaatt aactcatcga cagcagttgg
    64441 ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc
    64501 cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact gccttccaga
    64561 acccccgaca tcacacgcaa ggttggatct gcctgcagta ccggccctcg cgtggcctgg
    64621 gtttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct
    64681 ttcaccagtt tcagacgccc tactggcgca actgtatttg ccggatcgcc cggattgagc
    64741 gccgagattg tgccatcggc agcaatagtg atttccgctc cttccggcac agcaattggc
    64801 cccgcctcgc ctatcacctg atgcccctga attgtcagtt gcccggtggg atcaacctga
    64861 atgctgccat tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc cagccagcca
    64921 tcctgctgca acgcgacgtc cagcgggcgc gaggtgtaat ccattttgcc gggcgtcata
    64981 tctgcgcccg gcgttgacgc cgtgaccaac gtgcgcgtgg gcagagaaag cccttccact
    65041 ggcaccgcgc gtaaagcatt caactgcgcg cgaaaaccgg gcgttgaggc attggccaga
    65101 ttactggcgg ttaccgcctg ttgattcagt gtctggctgg ctgctcccat cgcggtataa
    65161 attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca gcgtgttgag
    65221 gatctggtcc tgagttttga tggtctgggc gttagactga tagttacgct gggcaacgat
    65281 catattgacc agttctttac tgagatcgac attggacgct tccaacgcac cgttggtcag
    65341 ggtgccaaag ttgcccgtcc cggctgtccc caacagcgcc acgccagaag attgcgtcgc
    65401 agaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac
    65461 aatctgcccc agcagttgtg tttgttcgtt ggaatagttg ccgacaaccg taccgtcatc
    65521 attgatttga taactcacca gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat
    65581 attgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa acgtggcggg
    65641 atctgcgccg ttaattgcgc cagtggtaat attctctgtt ggatttgagg tcagaacgcc
    65701 attggcatta aacaccagct tcattgcagg ctcggctgta cctgttggat cactgctatc
    65761 ctgggtgtag acctgccagt tattatcccc ggtcttcaca aagtagacgc tcatgtcatg
    65821 agcattaccc tgactgtcga aaacagtcac cgaacctttt ttgttatagc tatccgcatt
    65881 gctggcgctg aatggcgtaa cagtaggaag cggatcactg gaattcaggt tgatctgcat
    65941 cgacgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa tattggtcgg
    66001 attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaacccg tcagctgtaa
    66061 accttgcata ttcaccaggt tacggttttc atccagctta aattgtccgt tacggctgta
    66121 gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc
    66181 cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga taccggcaac
    66241 ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga
    66301 tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga ggttggtggc
    66361 ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct gaaatgtgaa
    66421 ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc cgagatccag
    66481 cgtattaccg ctgttgccgc ggagcacacc ctgcaccaga gcaaactgca gcggctgggc
    66541 aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac cgttcggcgc
    66601 agtgctgcca tcagtcaacg taccgtccca ggtgaaactg tggactccgg cggtcagttc
    66661 accaatatca atggtgcgca caaccgcgcc atttttatcg gtgatggtgg ccgtaacttt
    66721 gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc gtggtcacag ccccttcttc
    66781 actgccggtt cccgcaagaa cagtggtgcc ggggatcatc acgccgtgac cgatcaggtt
    66841 actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga gcgtggtatt
    66901 gagtttttca atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt
    66961 ttccattgga ttggtcgggt cctggttttt cagctgcgcc accaacaaag tcagaaaact
    67021 gctttgtaag tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc
    67081 ggtatttgtc ggatcggtgg tggttaccgc aatggacata gctttctcct ttattgaccg
    67141 agcgtaaggg ttttcagcat catgcttttc accgtgttga gcacttcaac attggcctga
    67201 tagctgcgtg acgccgacat ggtgttaacc atctccccga cgacatcaac gttcggcatt
    67261 tttacgtagc cctttgcatc cgccagcgga ttacccggtt cataaaccag tttgtccggg
    67321 gcctgacttt ctataacatc ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg
    67381 ttaacctgga ataccacctg ttttgcccga tatggctgtc catcgggacc ggtcacgcta
    67441 tcagcattcg ccagattact ggccgccacg ttcaagcgct gggactgggc agttaacgcc
    67501 gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct ctgtaaaacg
    67561 ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta ttgcaggctg
    67621 ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc
    67681 tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg cgccggaatg
    67741 tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc acgttgcatg
    67801 acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg ggtatcggca
    67861 ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag cgcctcttgt
    67921 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    67981 tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    68041 gctatttatg ccgttgatga ccattgcgga caggtacaat tcacgttgta gaaatggctg
    68101 ggggcgaaaa tgctggcaat aaaacgtagc gtggcgatca tctcgatact attcactccg
    68161 ttaagtgcgg cgagtaatct cacatcgcaa ttgcaaacct tttttagcgc ccagctcgcg
    68221 ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact accgccgtgc
    68281 gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg
    68341 gcacgctgcg gtaacgacaa acgatattta caggttaatg tacaggccac aggaaattat
    68401 gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa tgtcaaacta
    68461 aaacgcggac ggcaggatac cctgccaccg cgtacggtgc aggatatcaa tcaacttgtt
    68521 gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccagtttcgc
    68581 caggcatggc gggtaaaagc gggacaacgc gtcaacgtga tcgccagcgg tgacgggttt
    68641 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcacacagaa tgcgcgggtg
    68701 cgcatggtat cgggactggt ggtcagcggc gttgttgatg cagatgggaa tattcttata
    68761 aaccagtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    68821 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagcccgta
    68881 agcaccgttc aaccacgcga aaccactgac gcgtcggtaa cgaacacccg ggcggcaaaa
    68941 acaacagcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    69001 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac
    69061 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgctcaacga agcgcagcaa
    69121 gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc agatgtccgc
    69181 tgtgcttaac gatctcaaaa cggtaatgga tcaagaacag caacatctct ctatggggca
    69241 gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc tggcgacgct
    69301 ggattacctc gaacagttac gcaggaaaga acccaataca gcaaatagcg ttgatattag
    69361 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    69421 cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa tgttgaaacc
    69481 gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacaaccc atcgcggcgg
    69541 taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga atgcattgtt
    69601 acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt gcagcgaagt
    69661 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag ggcatggtac
    69721 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    69781 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta aagattttct
    69841 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    69901 gtgacagccc agcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    69961 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa taaaagccag
    70021 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    70081 gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc gaaacggtta
    70141 acggacccga aagaatgccc aacgcaaccg cactcggcag cgccaacagg aaacaaagac
    70201 gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    70261 acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag ggaaactcca
    70321 ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa
    70381 tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc ggtcccatct
    70441 gtttcaccac gcgcattgct ccggcatcgt ggaagttaat gcgcggcagg accagcatgc
    70501 cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg accgtaacag
    70561 cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa
    70621 tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg ttccacgtat
    70681 tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga aaggtaatct
    70741 ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca
    70801 ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg
    70861 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt
    70921 tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    70981 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    71041 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    71101 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccag acccacgggc
    71161 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctggatt atggtgaatt
    71221 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    71281 atacgttgcg ccagcactgc ctgttcgccg gcggtttgca gaactgtctg attttgcaca
    71341 cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt gctctgccaa
    71401 ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct cacgccattc gcgcatatcc
    71461 gtaatgtcga tgagcgcatc gtcagtcacg gcctgcacgg tttcacgctg acttccggca
    71521 cggcgatgca tacaggtggt gatctgcaaa ggatcggccg aaaaatggtg ctcggcaaac
    71581 agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc tttgccgccc
    71641 gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt gaaataaaga
    71701 tcgtgtggcc cgacactatt gctacgatgt ttatccattc ttagcgaagc tgcggtagcg
    71761 agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    71821 gtcagttttt tccgcgccgc cagttccacc agccgttcag catcgcgcag attttctgcc
    71881 agcggtttat cgacacagac atgtacaccc gcattgagta acgtactgac cacgtcaaag
    71941 tggctggcgg tgctggaatg cacaaaaacc gcatcgcaac tggcggcaag gctggataac
    72001 gaatcggcat aaggaatgcg ccagctttca caaattggca gggctttcgc gcgcgtaggc
    72061 gaccaggctc cttgtaacat ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    72121 tgcgcaatgc cgcctaatcc cacaacgccg atacgtaatt ttttcacttt aatctcccag
    72181 atgggccagc aacgaatcaa gacgctgttt cagttctgcc acttcgattt ccagggcttc
    72241 gacgcgggcc tgtaaatcac catcaaccgc gttcgacata tccgttaccg ccggctgatc
    72301 ctctacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    72361 cagacgcacc acaaaaggac catcttcgcg atttgccagt tgttccagcg tcgactccac
    72421 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt
    72481 ctgggcacca cgcaataaca acgtggtgat taacgccact tccgctgcgc tcagtttcag
    72541 atcgccaaat tctgaattac aaaaacgttg ttcatatttg gtgacccgat taccaaagcc
    72601 actcaccgtg cgcagataat ggcgtttgac cagattatcc agttgctcct gcacttcaga
    72661 ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg tgactacacc
    72721 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    72781 cacgcgagct tcaagggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    72841 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    72901 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc
    72961 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    73021 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    73081 tcgaataacc gagatagcag gcatgaaaag agccacgaac aacattggaa aaattggcaa
    73141 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct gaaccttgtt
    73201 tatgaaattc gttaatcatc cccagcctgg cctgccagcc tgatggataa cagtggcttt
    73261 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcaa
    73321 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    73381 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    73441 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc
    73501 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgat gttttgataa
    73561 aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    73621 tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta
    73681 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    73741 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc
    73801 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    73861 gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc ggattcgcca
    73921 caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc tcgggactcg
    73981 gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac
    74041 aacgttgtcg ttttttctcg ctgttgatga tgcaggacag tgccggtgcg gtcattggcg
    74101 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggccg
    74161 ttttgtttgt gctgtgtgcg gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    74221 ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca
    74281 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    74341 caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg tatgccattg
    74401 aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa aagcattttc
    74461 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    74521 gcatggtcag cggtctgcaa caacttttca ccctgatttg tctgttttat atcgggtcga
    74581 tcattgccga gcctgcgcgt gaaaccttaa gtgctttgct ggcggacgca agagctcgcg
    74641 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    74701 gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt ccgtggatga
    74761 tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agtcagaaac
    74821 gcgcaacgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc tgactcctcc
    74881 atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac atttacgatc
    74941 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    75001 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    75061 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat atcgttcact
    75121 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    75181 agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt tttgccaaat
    75241 cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac aagaaagagg
    75301 ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    75361 gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    75421 tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg gtagccggaa
    75481 tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag acacaaatca
    75541 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    75601 tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca tgctgcgttg
    75661 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    75721 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    75781 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    75841 ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgatcagcca aattggtcgc
    75901 gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa ctccctgttc
    75961 ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa
    76021 aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca accggaaaaa
    76081 atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg caattacttt
    76141 aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa
    76201 aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt tacggattaa
    76261 tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc
    76321 tttgtgtccg gcaaaaacat tccttcagcc ggagcataga gattaatgac tgcaccatcc
    76381 caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga tggcgacatg
    76441 ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt aatgcccaat
    76501 ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg tattcttgac
    76561 gccgtacctg ccgggcacga tttcacccca ttgatgacct gttatttaac agattcgctg
    76621 gatcctaatg agctggagcg cggatttaat gaaggcgtgt tcaccgctgc aaaactttac
    76681 ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc aatcatgccg
    76741 gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga agtgacacat
    76801 gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg
    76861 cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc
    76921 gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca gcatctgatg
    76981 tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg tttacccatc
    77041 ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg ttttaatcga
    77101 gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag cagttgcggc
    77161 tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag
    77221 atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca gttctatggg
    77281 ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt tgctgaaagc
    77341 atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt acgctggtcc
    77401 gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tatttaacct gtataaataa
    77461 ccagtatatt caacaggggg ctattatgcg aattgaagtc accatagcga aaacttctcc
    77521 attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta ttcagtatgc
    77581 gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata atttatcggt
    77641 tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg aaacgtggga
    77701 aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt ttgccgggtc
    77761 gccccggctt ttttatttta cttacgaatt ttgagtacaa aatttctaca cttcctcaaa
    77821 ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt tcatataaat
    77881 cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac ccttacttat
    77941 tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta
    78001 acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg
    78061 tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc
    78121 agaccccaaa taagtccgag caggaaggga ctgaagttgg tcaaacgctc tggttaacca
    78181 ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg
    78241 gtgatttcca ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag
    78301 gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct
    78361 gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg
    78421 gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga
    78481 ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc
    78541 gccatgctta tggtgaaggc gaaaagtatg tcccgctggt cctccgcgcg caaacgctgt
    78601 gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta
    78661 acctcggccc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc
    78721 aactcaacgt tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg
    78781 tcccggacaa ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg
    78841 cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc
    78901 cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt
    78961 atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc
    79021 tgcctgtcca gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg
    79081 tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt
    79141 ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt
    79201 cagcggatga acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt
    79261 tcttgcgcaa tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg
    79321 ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc
    79381 tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg
    79441 ccgattttgc gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct
    79501 tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg
    79561 tcggctgttg cactatctca
//
LOCUS       DAJTVK010000004        74366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000004 DAJTVK010000000
VERSION     DAJTVK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.004"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..1015
                     /locus_tag="OO372_000295"
                     /pseudo
     CDS             71..1015
                     /locus_tag="OO372_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="OO372_000296"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="OO372_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR6339909.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="OO372_000297"
     CDS             1797..1904
                     /locus_tag="OO372_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6339910.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="OO372_000298"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="OO372_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6339911.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="OO372_000299"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="OO372_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6339912.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="OO372_000300"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="OO372_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6339913.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="OO372_000301"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="OO372_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6339914.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="OO372_000302"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="OO372_000302"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6339915.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="OO372_000303"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="OO372_000303"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6339916.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="OO372_000304"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="OO372_000304"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6339917.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="OO372_000305"
     CDS             complement(11226..11813)
                     /locus_tag="OO372_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6339918.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="OO372_000306"
     CDS             11983..12561
                     /locus_tag="OO372_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6339919.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="OO372_000307"
     CDS             complement(12691..13452)
                     /locus_tag="OO372_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6339920.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="OO372_000308"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="OO372_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR6339921.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="OO372_000309"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="OO372_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6339922.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="OO372_000310"
     CDS             complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="OO372_000310"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6339923.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16807..17205
                     /locus_tag="OO372_000311"
     CDS             16807..17205
                     /locus_tag="OO372_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6339924.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17202..17897
                     /locus_tag="OO372_000312"
     CDS             17202..17897
                     /locus_tag="OO372_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6339925.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            18027..18911
                     /gene="cdd"
                     /locus_tag="OO372_000313"
     CDS             18027..18911
                     /gene="cdd"
                     /locus_tag="OO372_000313"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6339926.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            19061..19780
                     /gene="sanA"
                     /locus_tag="OO372_000314"
     CDS             19061..19780
                     /gene="sanA"
                     /locus_tag="OO372_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6339927.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19783..20022
                     /locus_tag="OO372_000315"
     CDS             19783..20022
                     /locus_tag="OO372_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6339928.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20216..21454
                     /gene="preT"
                     /locus_tag="OO372_000316"
                     /pseudo
     CDS             20216..21454
                     /gene="preT"
                     /locus_tag="OO372_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21448..22683
                     /gene="preA"
                     /locus_tag="OO372_000317"
     CDS             21448..22683
                     /gene="preA"
                     /locus_tag="OO372_000317"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR6339929.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="OO372_000318"
     CDS             complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="OO372_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6339930.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="OO372_000319"
     CDS             complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="OO372_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6339931.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="OO372_000320"
     CDS             complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="OO372_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6339932.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26695..27735)
                     /gene="galS"
                     /locus_tag="OO372_000321"
                     /pseudo
     CDS             complement(26695..27735)
                     /gene="galS"
                     /locus_tag="OO372_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27877..29034)
                     /locus_tag="OO372_000322"
     CDS             complement(27877..29034)
                     /locus_tag="OO372_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6339933.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(29051..29719)
                     /gene="folE"
                     /locus_tag="OO372_000323"
     CDS             complement(29051..29719)
                     /gene="folE"
                     /locus_tag="OO372_000323"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6339934.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29977..30813
                     /gene="yeiG"
                     /locus_tag="OO372_000324"
     CDS             29977..30813
                     /gene="yeiG"
                     /locus_tag="OO372_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6339935.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="OO372_000325"
     CDS             complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="OO372_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HCR6339936.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="OO372_000326"
     CDS             complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="OO372_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6339937.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34802..35683)
                     /locus_tag="OO372_000327"
     CDS             complement(34802..35683)
                     /locus_tag="OO372_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6339938.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35782..36831
                     /locus_tag="OO372_000328"
     CDS             35782..36831
                     /locus_tag="OO372_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6339939.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36905..37762
                     /gene="nfo"
                     /locus_tag="OO372_000329"
     CDS             36905..37762
                     /gene="nfo"
                     /locus_tag="OO372_000329"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6339940.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37766..38854
                     /locus_tag="OO372_000330"
     CDS             37766..38854
                     /locus_tag="OO372_000330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6339941.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38910..40160)
                     /locus_tag="OO372_000331"
     CDS             complement(38910..40160)
                     /locus_tag="OO372_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6339942.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="OO372_000332"
     CDS             complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="OO372_000332"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6339943.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41331..42029
                     /gene="yeiL"
                     /locus_tag="OO372_000333"
     CDS             41331..42029
                     /gene="yeiL"
                     /locus_tag="OO372_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCR6339944.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42096..43346)
                     /locus_tag="OO372_000334"
     CDS             complement(42096..43346)
                     /locus_tag="OO372_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6339945.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="OO372_000335"
     CDS             complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="OO372_000335"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6339946.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44366..45307)
                     /locus_tag="OO372_000336"
     CDS             complement(44366..45307)
                     /locus_tag="OO372_000336"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6339947.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="OO372_000337"
     CDS             complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="OO372_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6339948.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="OO372_000338"
     CDS             complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="OO372_000338"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6339949.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="OO372_000339"
     CDS             complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="OO372_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6339950.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49909..51090
                     /gene="setB"
                     /locus_tag="OO372_000340"
     CDS             49909..51090
                     /gene="setB"
                     /locus_tag="OO372_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6339951.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51087..51341)
                     /locus_tag="OO372_000341"
     CDS             complement(51087..51341)
                     /locus_tag="OO372_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR6339952.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51496..52068
                     /gene="yeiP"
                     /locus_tag="OO372_000342"
     CDS             51496..52068
                     /gene="yeiP"
                     /locus_tag="OO372_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6339953.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52291..53757
                     /locus_tag="OO372_000343"
     CDS             52291..53757
                     /locus_tag="OO372_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6339954.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53875..54861
                     /gene="yeiR"
                     /locus_tag="OO372_000344"
     CDS             53875..54861
                     /gene="yeiR"
                     /locus_tag="OO372_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6339955.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54900..55613
                     /gene="lpxT"
                     /locus_tag="OO372_000345"
     CDS             54900..55613
                     /gene="lpxT"
                     /locus_tag="OO372_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6339956.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            56025..56591
                     /gene="mepS"
                     /locus_tag="OO372_000346"
     CDS             56025..56591
                     /gene="mepS"
                     /locus_tag="OO372_000346"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6339957.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56772..58328
                     /locus_tag="OO372_000347"
     CDS             56772..58328
                     /locus_tag="OO372_000347"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6339958.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58410..60224
                     /locus_tag="OO372_000348"
     CDS             58410..60224
                     /locus_tag="OO372_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6339959.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60225..61319
                     /locus_tag="OO372_000349"
     CDS             60225..61319
                     /locus_tag="OO372_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6339960.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61319..62344
                     /locus_tag="OO372_000350"
     CDS             61319..62344
                     /locus_tag="OO372_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6339961.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62346..63935
                     /gene="yejF"
                     /locus_tag="OO372_000351"
     CDS             62346..63935
                     /gene="yejF"
                     /locus_tag="OO372_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6339962.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63939..64283)
                     /locus_tag="OO372_000352"
     CDS             complement(63939..64283)
                     /locus_tag="OO372_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6339963.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64616..65736)
                     /locus_tag="OO372_000353"
                     /pseudo
     CDS             complement(64616..65736)
                     /locus_tag="OO372_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="OO372_000354"
     CDS             complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="OO372_000354"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6339964.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66609..68369
                     /locus_tag="OO372_000355"
     CDS             66609..68369
                     /locus_tag="OO372_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6339965.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68494..68778
                     /gene="rplY"
                     /locus_tag="OO372_000356"
     CDS             68494..68778
                     /gene="rplY"
                     /locus_tag="OO372_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6339966.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="OO372_000357"
     CDS             complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="OO372_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6339967.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70106..70333
                     /locus_tag="OO372_000358"
     CDS             70106..70333
                     /locus_tag="OO372_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6339968.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70353..72113
                     /gene="pbgA"
                     /locus_tag="OO372_000359"
     CDS             70353..72113
                     /gene="pbgA"
                     /locus_tag="OO372_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6339969.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72188..72264
                     /locus_tag="OO372_000360"
     tRNA            72188..72264
                     /locus_tag="OO372_000360"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72222..72224,aa:Pro,seq:ggg)
     gene            complement(72367..74366)
                     /gene="yejO"
                     /locus_tag="OO372_000361"
                     /pseudo
     CDS             complement(72367..74366)
                     /gene="yejO"
                     /locus_tag="OO372_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18267 a        19590 c        18256 g        18253 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgtaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccagttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgctaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accaacaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcggt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgcaa ggagcgcgca
    15361 gggggcggcc aatcgccgcc gccccctgca cccccgggct ctggcgaaca aaatcgccgc
    15421 tgcgcggtgc cctcggctta tcccttacgg ctaccgggtc gggcgcgagg taacatccct
    15481 gtaaaacgcg ccctcagccc acctccatgt gggctgcccc ggccttcagg gaacgcctcg
    15541 gcaattttga cgccaccaaa caaccgtgcg gcctattgat aaagagctaa cacattgtca
    15601 aaaaacatca ctatggtttt ttagagtttc tcgatatcaa ttgcctgaat agcccttgca
    15661 atatcagggg aattattcaa cacccgaaca tgctgaaata attccgttgc ttcatcgtat
    15721 tctttacgca aataactcaa ccactgttta atccgcgcaa cgtgatataa cccggtatcg
    15781 ccctgctttt ccagacgggt atatttttgc agcaaagcaa ccacctccgg ccacggcatt
    15841 cgcggttcgt tatattttac tacccggctc aggttgggaa tattgagcgc cccgcgacca
    15901 atcatcactg cgtcgcagcc gctgatcgca atgcattgtt gcgcgctctg ccagtcccag
    15961 atttcgccgt tggcaatcac cggaatattc agccgctggc gaatctcgcc aatcgcctgc
    16021 cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc gtccatgcac caccagctcc
    16081 gtagcaccag cctgttgaac cgcatcggcg atttcaaatt tcttctcacc gctgtcccag
    16141 cccagacgca ctttcacgct gacgggcaaa tgcgccggta cagcttcacg catcgctttt
    16201 gcaccctggt agatgagttc aggatctttg agtaacgtcg ccccgccgcc gctgccgtta
    16261 accgttttcg acgggcagcc gcaattgaga tccacgcccc aggaacctaa ttccaccgca
    16321 cgggcggcgt tctctgccag ccattgtggg aactgaccta acaactgaac gcgcaccagc
    16381 gtaccggatg gtgtccggct ggcgttttgt agctcagggc aaatgcgatg aaagactttt
    16441 accggcagca gttgatccac cacgcggaca aactcggtga tgcacagatc gtagtcgtta
    16501 acttcggtca gcaattctcg caccagagag tcaagcacac cctccatcgg agccagtaac
    16561 acacgcatat cttcacccgg aaaaaaatga ggcgctatgt tagcgcctat ggtcaacgga
    16621 ttaaaggtct gcaaagaggg attgctgcaa actgtaatcc ctctctatgc ttgatgtatg
    16681 cctgagttcg ttgcgcattg cgtaatgccg gggagcgaaa aaataagcgc tgatttctta
    16741 atgtgatcgg tagcacgttt tacgaattaa ttgtatgatg aatccatctc atctggggtg
    16801 ttgattatga gtaagacact gaacattatc tggcaatatt tacgcgcttt cgtcctgatt
    16861 tatgcctgcc tgtatgcagg cattttcatt gcttccctgc taccggtaac cattccgggc
    16921 agcatcatcg ggatgctgat cctgtttgtc ctgctggcct tgcaaattct tccggcaaaa
    16981 tgggtcaatc cggggtgcta cgtactgatt cgctatatgg cgctattgtt tgtgccgatt
    17041 ggcgtaggcg tcatgcaata ttttgatttg ctccgcgcac agtttggccc ggtagtggtt
    17101 tcctgtgcag tcagtacgct ggtggttttt ctggtggtga gctggagttc acaactggta
    17161 cacggtgaac gtaaagtcgt aggtcagaaa ggatcagaag aatgatggcg aatatctggt
    17221 ggtcattacc gctgacgttg attgtctttt ttgccgcccg caaactggcg gcacggtata
    17281 agtttcccct gcttaacccg ttgctggtag caatggtggt gatcattcct tttttaatgc
    17341 tgaccggcat ctcttacgac agctacttta aaggtagcga agtgctaaac gatctgctgc
    17401 aaccggcggt cgtcgcgctg gcctatcctt tatatgagca gctacaccag atccgcgcgc
    17461 gctggaaatc gatcatcacc atctgtttta tcggcagcgt ggttgcgatg gtgacgggga
    17521 cttccgtggc attgttgatg ggcgcttcac cggaaatcgc cgcgtcaatc ctgccgaaat
    17581 cagtcaccac gcctattgca atggcggttg gcggcagtat tggcggtatt ccggcaatca
    17641 gcgcagtttg cgtgattttc gtcggcatcc tcggcgcggt atttggccat acattgctta
    17701 atgcgatgcg tattcgtacc aaagctgcgc gcggtctggc aatggggaca gcctcgcacg
    17761 ccctcggtac ggcgcgctgc gccgagctgg attatcagga aggtgcattt agttcgctgg
    17821 cgctggtgtt atgcgggata attacttcgc tgatcgcacc gttccttttc ccgattattc
    17881 tggcagtaat gggctaaaat ttgcgatgcg tcgcgcattt ttgatgtaag tttcacgcgt
    17941 tgcataatta atgagattca gatcacatat aaagccataa cgggttcgta aactgttatc
    18001 ccattacatg attatgaggc aacgccatgc atccacgttt tcaaaccgct tttgcccaac
    18061 ttgcggataa cttgcaatct gcactggaac ctattctggc agacaagtac ttccccgctt
    18121 tgttgaccgg ggagcaagtc tcatcgctga agagcgcaac ggggctggac gaagacgcgc
    18181 tggcattcgc actacttcca ctggcggcgg cctgtgcgcg tacgccattg tcgaatttta
    18241 atgttggcgc aattgcgcgc ggtgtgagcg gaacctggta tttcggtgcc aatatggaat
    18301 ttattggtgc aacaatgcag caaaccgttc atgccgaaca aagcgcgatc agccacgcct
    18361 ggttgagtgg tgaaaaagcg cttgcagcca tcaccgttaa ctatacgcct tgtggtcact
    18421 gccgtcagtt tatgaatgaa ctgaacagcg gtctggatct gcgtattcat ctgccgggcc
    18481 gcgaggcaca cgcgctgcgt gactatctgc cggatgcctt tgggccgaaa gatctggaga
    18541 ttaaaacgct gctgatggac gaacaggatc acggctatgc gctgacgggt gatgcgcttt
    18601 ctcaggcagc gattgcggcg gcaaaccgtt cgcacatgcc ttacagtaag tcgccaagcg
    18661 gtgtcgcgct ggaatgtaaa gacggtcgta ttttcagcgg cagctacgct gaaaacgccg
    18721 cattcaaccc gactctgcca ccgttgcagg gggcgttaat tctgttgaat ctcaagggtt
    18781 atgattaccc ggatatccag cgcgcggttc tggcagaaaa agccgatgcg ccgttgattc
    18841 agtgggatgc cacctccgca acgctgaaag ctctcggctg tcacagtatc gaccgcgtgc
    18901 ttctcgctta agcctggtgc cggatgcggc gtgaacgcct tgtccggctt gccagccctc
    18961 tcctggtgtc gaaattcccg gcaaacagtt tgccgtttct tgcgcaaaac cagcgggtaa
    19021 agtagcctga tggaaatttt ccttagatcg agtctcctgc atgttaaagc gcgtgttcct
    19081 cagcctgtta gtcctgatcg gcttgctgct gttgactgtg ctcggcctcg atcgctggat
    19141 gagctggaaa accgcgcctt atatctacga cgaattgcag gatctcccct accgccaggt
    19201 cggcgtggtg ctcggcacag caaaatatta tcgtactggc gtaattaatc agtattatcg
    19261 ctaccgcatt caaggagcga ttaatgccta taacagcggt aaggtaaatt atctattact
    19321 gagcggcgat aacgcattgc aaagttataa tgagccgatg accatgcgca aagatttaat
    19381 cgctgctggt gtcgacccat cagatattgt tctcgattac gcaggctttc gtacgctgga
    19441 ttccatcgtg cgtacacgca aagtgttcga tactaatgat ttcattatta tcacccaacg
    19501 tttccactgt gagcgggcat tatttattgc gctgcatatg gggattcagg ctcagtgtta
    19561 tgccgtaccg tcaccgaaag atatgctgtc agtacgtatt cgcgaatttg ccgcccgttt
    19621 cggtgcgctg gctgaccttt atatttttag acgtgaaccg cgttttttag ggccgctggt
    19681 ccctattccg gctatgcacc aggtgccgga agatgcgcag gggtatcccg ccgtcacacc
    19741 cgaacagtta cttgaattac aaaagaaaca aggaaagtag ttatggatgt acagcagttt
    19801 tttgtcgttg ccgttttttt ccttatcccg atattttgtt tccgcgaagc atggaaaggc
    19861 tggcgcgcag gcgcgattga taaacgggtt aaaaatgcac cggaaccggt gtatgtctgg
    19921 cgagcaaaaa atcccggact ctttttcgct tatatggtgg catatatcgg cttcggaatt
    19981 ttatctatcg gcatgattgt ttatcttatt ttctatcgtt aatattttct cttttctgtt
    20041 aattattgag aataattatt acttcactca ataagctgcg gatataacat tctgaaccgc
    20101 agctatttgt gaatcctttc acagtttaaa tttccccaca cgcttagcct taatatcagc
    20161 acgcaatatt tatttagcgt tcgccttaat tactcatagc attaaggaag atcacatgcc
    20221 gcaacaaaat tatctggatg aactcactcc ggcttttacg cctttactgg cgattaaaga
    20281 agcctctcgc tgtttattat gtcatgacgc tccctgtagt caggcttgcc cggcacagac
    20341 cgatccgggg aaatttattc gctcaatcta ctttcgtaat tttaaaggcg ctgccgagac
    20401 aattcgcgaa aataatgccc tcggtgccgt ttgtgccaga gtgtgcccga cggagaaatt
    20461 atgccaaagc ggttgtaccc gtgccggtgt tgatgctccc attgatatcg gccgcttaca
    20521 gcgttttgtt actgattttg aacaacaaac cgggatggaa atttatcagc ccggtactaa
    20581 aacgctcggc aaagtcgcga ttattggcgc aggtcctgcc ggattgcagg ccagtgtgac
    20641 actgacaaac cagggttatg acgtcacgat ctatgagaaa gaagcacaac ccggtggctg
    20701 attgcgtaac ggcattccgc aattccggtt accgcaatca gtactggatg cagagatcgc
    20761 ccgtattgaa aaaatgggcg tgaccattaa gtgcaacaac gaagtcggta acacactcac
    20821 ccttgagcag ctgaaagcag aaaaccgcgc ggtactggtc accgtggggt tatcaagcgg
    20881 ttccgggcta ccgctgtttg agcatagtga cgttgagatt gccgtcgact tcttgcaacg
    20941 tgcacgacag gcgcaaggcg atatcagcat tccacaaagc gcattaatta tcggcggcgg
    21001 tgatgtcgcg atggacgtag ccagcacgct gaaagttctc ggctgtcagg cggtaacttg
    21061 cgtagcgcgt gaagagttag atgagtttcc ggcaagcgaa aaagagttta ccagcgcccg
    21121 ggagctgggc gtttcgatca tcgatggatt cacgccagta gccgtggaag gcaataaagt
    21181 cacgtttaag catgtacggt tatcgggcga actgacgatg gcggcagata aaattattct
    21241 cgccgtcggt cagcatgcca gactagatgc ctttgcggag ttagagccgc agcgtaacac
    21301 catcaaaaca caaaattacc agacccgcga cccgcaagtc tttgctgctg gcgatattgt
    21361 tgagggagac aaaaccgtgg tctatgccgt gaaaaccggg aaagaagccg ccgaggcgat
    21421 tcatcactat ttagagggag cttgctcatg ttaacgaaag atctttcgat tactttttgc
    21481 ggcgtgaagt ttcccaaccc gttctgcctc tcttcttcgc cggtaggcaa ctgctatgag
    21541 atgtgtgcca aagcctacga cacaggttgg ggcggtgtgg tgtttaaaac gatcggcttt
    21601 tttatcgcca acgaagtctc gccgcgtttt gatcatctgg tgaaagaaga taccggtttt
    21661 atcggcttca aaaatatgga gcagattgcc gagcatccgc tggaggagaa tctggccgcc
    21721 ctgcgtcggc tgaaagaaga ttacccggac aaagtattga tcgcttcgat catgggggaa
    21781 aatgagcagc aatgggagga gctggcgcgc ctggtgcaag aagctggcgc ggatatgatc
    21841 gagtgtaact tctcctgtcc gcaaatgact tctcatgcga tgggtagcga tgtcgggcaa
    21901 agcccggagc tggtagaaaa atattgtcgg gcagtgaaac ggggttccac gctgccaatg
    21961 ctggcgaaga tgacgccgaa tatcggtgat atgtgcgaag tggcgctggc ggcgaagcgc
    22021 ggcggcgcag atggcattgc ggcgattaac accgttaaat ccatcaccaa tatcgatctt
    22081 aatcagaaaa tcggtatgcc gatcgttaac ggaaaatcaa gtatttccgg atattccggt
    22141 aaagcggtaa aaccgatcgc cctgcgcttc attcagcaaa tgcgcaccca tccagaactg
    22201 cgcgatttcc caatcagcgg tatcggcggc attgaaacct gggaggatgc ggcggagttt
    22261 ttattgctcg gcgcagcaac gttacaggtc accaccggca ttatgcagta cggctatcgc
    22321 atcgtagaag atatggcgag cgggttatcg cattatctcg ccgatcaggg atttgattcg
    22381 ttgcaggaga tggtaggtct ggcgaataac aatattgtcc cggcggaaga tttagaccgc
    22441 agttatattg tctatccccg tatcaatctt gacaaatgtg ttggctgtgg acgctgttat
    22501 atttcctgtt acgacggcgg tcaccaggcg atggaatgga gcgagaaaac ccgcacaccg
    22561 cattgtaata ccgagaaatg tgtgggttgt ctgctttgtg gtcacgtctg cccggtgggt
    22621 tgtattgagc tcggggaagt gaagtttaag aaaggcgaga aagaacaccc ggtaacgttg
    22681 tagaacccgc gtcacatccg gcatttggtt cctgttgccg gatgcggcgt gaacgcctta
    22741 tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg aatgcggacc
    22801 acattcacat catttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat
    22861 aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata
    22921 gttgatgacg gtaaaaataa tcaccccggt caccacgcca atcaccgtcc ccacaccgcc
    22981 gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc
    23041 gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa
    23101 cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt
    23161 tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca
    23221 caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa
    23281 actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat
    23341 tggcgacgcc ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt
    23401 gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa
    23461 accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc
    23521 catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac
    23581 tgccgccagc cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc
    23641 tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt
    23701 caacagactt aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac
    23761 gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat
    23821 gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt
    23881 tgtttttgta tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt
    23941 tatccctaac aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc
    24001 cagttcggca attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg
    24061 ttcatcgagc attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg
    24121 ctgattacca ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat
    24181 cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt
    24241 atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata
    24301 aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccgtggttta tggcttcgtt
    24361 ggcattatgg ttattgatct gtttgccgtg caacgtaatg gtgccagccg atttctcgcg
    24421 aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagcc cggcaatacc
    24481 gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga
    24541 cgttaggtta cgcacctcga ggatgacttc gcccggcttg ttttctttgt caggaaaacg
    24601 ctgattaaga gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag
    24661 cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa
    24721 gatttcttcc attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg
    24781 aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat
    24841 aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga
    24901 aacggataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt catcaaaaat
    24961 tgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg
    25021 ccccagccac atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac
    25081 catcgaaata ccattttcca gtgcctcttt ggcggaatgg aaatcgatct ctttaccctg
    25141 gaataaaatg gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga
    25201 ttttcctgca ccgttttccc ccattaatgc atggatagaa tgtggccgga cttttaaatt
    25261 aacgttatca agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa
    25321 gtattccccg gaagacggag tcgttgagct gaccatataa ttttaccttg ttggccatac
    25381 aataagggcg cagtaaacga ctgcgcccaa tcagtcttat ttcttgctga attcagccag
    25441 gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt
    25501 ggtgccatca gccgcacctt tgccatcggc caggtttttc gccagatcaa aggtcgcttt
    25561 cgcctggttg ttagcatcgt tcagtactgt gcccgccagt gcaccggatt tcaccagcgc
    25621 cagcgcttct ggcagcgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt
    25681 cagtgcttct accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt
    25741 gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtgtccca
    25801 cattgcggta tctaactgta actgttcagt tttgatgcct ttatcgttca attctttaat
    25861 cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcagtacgaa
    25921 ctgaatctga ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat
    25981 caaatcgccc tgaataatgc cggactcttt ggagtcagtg ccaacgtagt aggctttgtc
    26041 gtagctatcc agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg
    26101 cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag
    26161 tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt
    26221 ctgagaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc
    26281 cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt
    26341 atcagcagcg tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa
    26401 caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt gagataaatg
    26461 ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac ttttttaaat
    26521 tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctat
    26581 tacctcggtg aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa
    26641 tcatagctat cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat
    26701 cgcctgattt gttgagttag tgatcgccgc cgcattctgg cgcgttgcga cagaatggcg
    26761 acgcactaac gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc
    26821 ctgcaaggcc agttcggtgg ctaatttagc cattgaagca atgggataac gcacggtcgt
    26881 taattgcggg tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg
    26941 taacggaatc gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc
    27001 gttataagca aatatagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc
    27061 ctcaccgccc ggcatgtccg gcgtaccagt gccaatccag ctttccggcg gaataatatc
    27121 ctgctctttc aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg
    27181 actggaagaa agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat
    27241 tcgggcaccg ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg
    27301 gttgattaac accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa
    27361 tgcttttgag tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg
    27421 acgctctttt tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg
    27481 agcgactaga tccaccgctt ttaccagcgc gccgaaaaac gcatcagaaa cgtccatcac
    27541 caccacgcca atggtgtcgc taacctgagt tgccagcgcc tgggcattgg cgtttggccg
    27601 ataatccagc tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt
    27661 gctgttattg agcacccggg aaaccgttgc cacagagacg ccagcctaac gcgctacatc
    27721 acgaatggtg atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc
    27781 ggctattttg tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg
    27841 ttacagccgt tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc
    27901 cgttccggca gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa
    27961 gtaacgcagc cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc
    28021 cagcagctcc aggcggtcaa aatgcataaa caaaccgagg tggtaaaaaa gcatggtaca
    28081 aatcagcgtt tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgatcgcaag
    28141 caccagctta aagcggctca attgcggcca gaagccataa aacagcgacg catagccaat
    28201 cgcctgaaac ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata
    28261 cgcccagtcc agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc
    28321 caccagcaca aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc
    28381 gctgcgcatc agcgccgcac caatgagcat catccctgcc agttgccagc catactgtgc
    28441 gcccagtgcc agtaaactgt tgccaacacc atcggcacga ttactgatcg cttcaacgcc
    28501 gccgtgaagc ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggtgcg
    28561 gctggtctgg ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata
    28621 aagcatgacg cctgtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca
    28681 gcagattaag cccaccagcc cgtaagccag cagaatatcg ccgtcccaga acaataaacc
    28741 gtgaataaaa cccagcaaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg
    28801 gggcagcaac atttgcaggc ccgcaccaaa cagcagcgca aaaagcgtga ggaatttcac
    28861 ctggccgatg agatcgagaa atgcccaggt ccatgcatcc tgcggcgtaa tagcgccgta
    28921 ccaggcggga ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag
    28981 gatccccaga atggcgacgc cgcgaacaaa atcgagcgtt acgttgcgct ccatggttcc
    29041 tgccttttaa tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac
    29101 tggatttgaa caatccacca agagaggtcg ttgttgtggc actggttgca tcgcggatgc
    29161 cacgcgcttt cacgcagtaa tgcaccgcat caatggagac agccacgtta ttggtgccca
    29221 gcagcgtttg tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct
    29281 gggcaaagaa ctgcacgata cggttaattt ttgacagacc gatcaccgaa tctttcggga
    29341 tataagccac cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtactggtca
    29401 gagtgatatc gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga
    29461 ttttcgggaa gttggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga
    29521 tgcgatgcgg cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga
    29581 tttcggtcat atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg
    29641 gcgggcgcag cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt
    29701 ctttactgag tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg
    29761 ggggcaaagt tattaagctg atttacagcc tgattattgt gcgtgaggcg gcgcacataa
    29821 tccagtattc acagcgataa ttattgtaat tgccgctgac tttcatcagc agatgttaaa
    29881 acatcgttat gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga
    29941 tgaagaaatt gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc
    30001 tgttttgaag gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg
    30061 acgttcagta tctttctccc accccctcgt gatcacaccc cgccaccagt gctgtactgg
    30121 ctttccggat taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgcgtg
    30181 gcggcagaac tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt
    30241 gccaacgacg atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa
    30301 ccgccgtggg cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg
    30361 gttcagtcgc aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt
    30421 cacggtgcgc tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt
    30481 gcgccaattg tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cacctattta
    30541 ggtgaggaca aaaatgcatg gctggaatgg gatagttgcg cgctgatgta tgccagtaac
    30601 gcgcaggatg cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac
    30661 cagttgcaac ctgcggtatt ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg
    30721 cgtattcagc cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac
    30781 ctgcgcttcc atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt
    30841 ctcatcagaa gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt
    30901 agtcgtcacg actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt
    30961 ctttagtcac ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag
    31021 ccgtcgcgct gtcggcgcgt ttctgcccgg tatagtgacc agaaacatag aatgaccagt
    31081 cttccagcgc cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg
    31141 atagcggttt gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg
    31201 acagtttcca ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag
    31261 ctttgttaac gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa
    31321 aaccaacaaa gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga
    31381 tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc
    31441 cttcttcacc catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat
    31501 ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa
    31561 gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt
    31621 cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat
    31681 cgtccatacg cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca
    31741 caaacagcgc gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg
    31801 catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg
    31861 tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt
    31921 tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat
    31981 cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg
    32041 agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat
    32101 tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg
    32161 tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc
    32221 tgccgtaagc tttcattccc agcacaccat caattaatgg tccactggta aagaactgac
    32281 cgttataggt atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac
    32341 ccgaccattt ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg
    32401 aaccgtacag cgacgacatc gggccacgga ccacttcaat acgttcgatg gaatcgaccg
    32461 ggatccagtt cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttcacgcgtt
    32521 taccgtcgac gagaatcagg gtatagctgc tgtccagacc acgaatacta acgcccttac
    32581 ggttatcccc ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat
    32641 tctgtaccgg ttttcgctgc agatcttcct gggtaatgac gctgatactg gcgggtgcat
    32701 ctttaaggtt ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca tcgtcatcga
    32761 ccgctaacac aggccataca caagaaatag cggacaaaca cagcccgacc cgtacgaaag
    32821 ggttcaacct aaacattcca tatctccatg aggtaactac gaaaataaaa tgggtatcgc
    32881 tcacatcttc tttacgtccc cttgcgtacg gcagcatcgc ggtggactta tttgtatgca
    32941 ggtgatcatc ccagaaagcc ggaccagcct ctgatttgtc agcctttttt gattgtagcg
    33001 taacgataat gcaaacgata acaattatca atccaaatgt taaattttat atccgcatga
    33061 tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag caaggaaggg
    33121 aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtgag
    33181 ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg
    33241 ttgccgctac gccgccccag tcaatggtat ctttcaggaa cgcttcgtag ttctggccca
    33301 aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag aaacctgaac
    33361 ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt
    33421 ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg ttcagcagcc
    33481 acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacaaaccg gcaatcaccg
    33541 tcgtcgcata cagcgcatta cgcggcacgc caccacgcga caatttagcg aaaatgcgcg
    33601 gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt
    33661 tacctgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc gcagagagca
    33721 gacccgcgtg ctggaacacc aaggtgaacg gactgacgct gatgtcttta acatcgttac
    33781 gcagcaggct cggatcggta tacggaataa tcaggctgat aatcaggatc gcgaacacat
    33841 agaacaacag gattcgccag aatacctgac gtaccgcgcg tggaatgttt ttcgccggat
    33901 cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa
    33961 tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc
    34021 agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa
    34081 cgatgataaa gacaataact gtcgtgactt tgatcagtga aaaccagtat tccgcttcac
    34141 caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac aacgcactcc
    34201 agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgtgct gcaaccaagt
    34261 caacggcaat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc
    34321 cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag
    34381 ccaattcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc atatacgaga
    34441 gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg
    34501 taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta
    34561 agcccggcgc ttctgtagtt ttagtttcgg aacccataca aaaatgctat ccatcttaaa
    34621 aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa
    34681 gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat
    34741 gcatgctgag ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcac
    34801 cttaacgcgg tacattcgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa
    34861 ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggg agagggaccg
    34921 caacttcact taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac
    34981 taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg
    35041 ccatctcaaa cttcggtaaa tgtgacagca acagataatc gacaatctcc cgcgtgccgg
    35101 aaccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg accggaccac
    35161 gagccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag
    35221 aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca
    35281 gcaccgcttg catcacgtcc tggctgttcc caacgctaag ttcaatcggc aactgcggat
    35341 aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg
    35401 catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt
    35461 caagcaatgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca accagtcttt
    35521 tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc
    35581 tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat ccacttttca
    35641 atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat
    35701 aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat
    35761 gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg
    35821 tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt
    35881 ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg
    35941 gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg
    36001 ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg
    36061 ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt gacgctgtcc
    36121 agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc
    36181 agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag
    36241 tcggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc
    36301 gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt
    36361 ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg
    36421 gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc
    36481 gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg
    36541 gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgtc
    36601 gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg
    36661 ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc
    36721 gcgctgaaaa aagctggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg
    36781 attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca
    36841 tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc
    36901 tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat
    36961 tcgcgccgtc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg
    37021 gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata
    37081 tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc
    37141 ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga
    37201 acaactgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga
    37261 agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg
    37321 tgtaacagcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga
    37381 acatctcgcg gcgattatcg acggcgtgga agataaattc cgcgtcggcg tctgcattga
    37441 tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgaaaaaac
    37501 gttcgctgat tttgcccgta ttgtcggctt taagtatctg cgcgggatgc accttaacga
    37561 tgcgaaaagt acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat
    37621 cggtcatgat gcgttccgct ggatcatgca agacgaccgt ttcgacggca ttccgctgat
    37681 ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca
    37741 aactgaaaaa gcggtggcct gatcgatgaa cgacagggaa aagcagattc ttaaaatttt
    37801 acggcgtaac ccgctgattc aacagaatga aatcgccgat attctgcaaa tcagccgctc
    37861 acgtgttgcc gctcatatta tggatttgat gcgcaagggt ttgattaaag gcaaaggata
    37921 tatccttacc gagcaggatt actgtgtggt tgtgggtgca atcaatatgg atattcgcgg
    37981 aatggcggat atccgttacc cgcaagcagc ctctcatcca ggttctgttc actgttccgc
    38041 aggcggcgtc ggacgtaata tcgcgcataa cctggctctg cttggtcggg atgtgcattt
    38101 aatatcagct attggcaacg atttttacgg cgagacatta ctggaagaaa cgcgccgcgc
    38161 gggcgtgaac gtttcaaact gcattcgtct gcatggacac agcactgcaa cttatctcgc
    38221 tatcgctaat aagcaggaag aaactatcct ggcaatcaat gacacacata ttctgcaaca
    38281 gctaacgcct caactgctca atacttcacg ggacttgatt cgccatgctg gtgtggtgct
    38341 ggctgattgt aatctcacac ctgaagccct ggagtgggtc tttaccattg ctgatgaaat
    38401 tccgatgttt gtcgataccg tttccgaatt taaagcgaac aaagtaaaaa actggtacag
    38461 ccggatccac actctgaaac caacacaaaa tgaactggaa attttgtggg ggcaaccgat
    38521 taaagacgac aacgatcgca tcagagctgt aaattcgctg catcaacaag gtgttaaacg
    38581 catttttgtt tatctgaaag atgaatctgt attttgcagc gataaggatg gagaacaatt
    38641 tttgctgacc gcgccagcgc atacgacagt agacagtttt ggtgctgacg atggttttat
    38701 ggcgggcctg gtatatagct ttcttgaagg atacagtttc cgcgacagcg cccgttttgc
    38761 ggtagcctgc gcggcaattt cgcgcgccag cggcagctta aacaacccta ccctgtctgc
    38821 cgataacgcg ctttcattag tgccaatggt gtaacaatgt tgccggattc gacgcttgac
    38881 cgcatcttat ccggctcata aattgcgcac tacgctaaac caataaagaa tcctgcaata
    38941 gtagcactca tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc aagttgggcg
    39001 atttccggcg cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa
    39061 ccaaagttag cgaaaccgca caacgcgaaa gaaataatcg ccacggtttt agcatccaga
    39121 gtgccacccg tttgcagata tggcgagaaa ttgagataag cgacaaattc attgatcgcc
    39181 agcttctgcc caatcaaact cccggcaaga tttgcatcac tccagtcaac ccccatcacc
    39241 cacgccaatg gggccaacag gtaacctaaa atcgactcca gcgaagcatg ttcaaaacca
    39301 aaccagccgc caacaccgcc gataataccg ttaatcaacg caattattgc aacaaatgcc
    39361 ataacaactg tcgctacacc ggcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg
    39421 gcttcaataa tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa agaaacctgc
    39481 gaagattccg tagccgggct taacaggcgg gcaaacaaga tcccgccagg gatcgccatt
    39541 aacgatgccg ccagcaaata ttccacaggt acgcccagtg cggcgtaacc aatcattgtc
    39601 gaaccagcaa tcgaggccat gccactacaa atcgctgtaa ataattcatt gcgattcaga
    39661 cgatcgataa agggtttgac gatcgccgga atttcgtttt gcccgaggaa aatggtggtg
    39721 accgcgacga atgactcgat cttgctgata tttaaagctt tctggaagat accgccgaga
    39781 atgcgaatta agatccccat cacaccgatg tagtagagaa tactcaccag tgcagtgacg
    39841 aagataattg ccggtaatac cctgaaacca aagataaatc ctgcgccatc aaataaggta
    39901 tccattttcg gtccgaccag agaaccgaag ataaatgcgc tacctgcgtc gctgtacgcc
    39961 atcactttgt ggacgccaaa agcgactttt tctgcgaccc aacgccctgg cggtaaccaa
    40021 agcataatcc cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg
    40081 atcttcttct tgtttactga cagtaaaaac gcaattgcca gcagtaccac cattcccaga
    40141 acacttctca tgacatccat agtgattatc tcttcatgtc attttgggcg acggctttct
    40201 gcccatcaga aatagccggg cctgcatctt gcgatactgt cccggcttgt tatcaacact
    40261 taatgggttt ttatgtagcc gcgtacgcac tcttcgacta atccccagaa ccagtcggta
    40321 tcaatagtga tgccgacttt agtattggcg ggcttgccaa gaacgcccag ctcgtcgcag
    40381 acggtacgcc catagcaagg gccactgttg acgtccactt cgacgtacat ctcctgggtt
    40441 ttaatgccat cagggttaat cagataaccg atgcaggtgg cgtcgtgcac cgggccgcca
    40501 gccaggccgt agttttcgaa ctgcgttttg agagtgaagt tcatgatgtc gctgaacatc
    40561 tctccggcag ggccgcctgc cctttccatt cgtgcaatta cgtccggggt gcaaacggtc
    40621 tggttggtaa gatcgaggcc catcatcact aatggaacgc cggaggtgaa caccacgcgt
    40681 gcggcttccg ggtcggcaaa gatattgaac tcggcagatg gggtgaagtt gcctgtaccg
    40741 taagcgccgc ccattagcac aatttcacgg attttgggca ggattgcggg ttgcatacgc
    40801 attgccaccg cgatatttga aagcggacca accggcacca gagtgatatc gccatcgctt
    40861 gccataaggg tatcaatgat gtatttcacc gcgtgggtac tttctgcctg gcgggtcagc
    40921 ggctcgaata ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt
    40981 tgacgcataa tgggttgcgg catccccgca taaaccggaa cattaatctc cagtttctgg
    41041 caaacattca ggccattaat taatgtttta tcaagcgtct gattacccgc aacaatggtg
    41101 atacctaata aatcaatcgc cggatgtttc gccgccatca ttatagcaat agcatcatca
    41161 tgacccggat cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc
    41221 cagttgcgtt attttttcta cagcaacgaa atttcgatcg ggccgatgat tgaatcttaa
    41281 caacagcgta cgaatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa
    41341 tccacaataa tgatcttaag cagcaattga tgagtgaatc cgcgtttaag gattgctttt
    41401 taacggatgt ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc
    41461 aggaagggca acagccgtcc tggctgtttt acctgacgcg aggccgcgcc aggctttacg
    41521 ccacgctagc taatggtcgc gtgtcgctga tcgatttctt tgccgccccc tgttttattg
    41581 gcgagattga gttaatcgac aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt
    41641 gttggtgcct tgcgctccct atgaaacatt accgcccgct gttattaaac gacacgctat
    41701 ttttacgaaa actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa
    41761 ctcagaatca atcatttccg ttagtgaatc gcctggcagc atttatatta ctctcgcagg
    41821 aaggtgatct ttatcacgaa aagcatacgc aagcggcaga gtatttaggc gtttcttatc
    41881 gacatctttt atatgttctc gcgcaattca ttcacgacgg tttattaatt aaaagcaaga
    41941 aagggtatct aattaaaaac agaaagcagt tgtcaggact ggcgctggag atggacccgg
    42001 agaataaatt ctccgggatg atgcagtaaa aattattggc aatagcacga ttgccggatg
    42061 caacgcttaa taaggcgtac atccggcaat gaaactcaag ctaaaccaat aaagaacccg
    42121 gcaatggtcg cgctcatcag gttggaaagc gtcgccgccg ccagcgcccg taaaccaagc
    42181 tgggcgattt ccggcgcgcg ttttggcgaa atagccgaaa acgctccaac gacaacaccg
    42241 atagaaccaa agtttgcaaa cccacagagg gcgaacgaga taatggcaat ggtcttgaca
    42301 tccagcgtgc cgctcgtttg cagatagggc gagaaattga gataagcaac aaactcatta
    42361 atcgccagtt tctgcccaat caaacttccg gcaagatttg catcactcca gtccaccccc
    42421 ataatccatg ccaacggggc cagcacatag ccgaaaatgc tttccagaga gacattggca
    42481 aaaccaaacc agccgccaac tccaccaata atgccgttga tcagcgcaat gatcgctaca
    42541 aatgccatta ccaccgtcgc cacaccggcg gcgattttca gcccggtcat tgcaccgttc
    42601 gccgcagctt caataatgct ttttggcggt gtttcgctaa acgacagatt ttcaaacgta
    42661 acttgcgaag gttccgtcgc cggactaaga atacgtgcaa acaggatacc gccaggaata
    42721 gccatcagcg aagcggctaa caaatagtca atcggcaccc ccattccggc atagccaatc
    42781 atcatcgaac ccgcaataga cgccatgccg ctacaaatag ccgtaaatag ctcgttgcga
    42841 ttcatgcgat caataaaagg tttaacgatc gccgggatct cattttgccc gaggaaaata
    42901 gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg aaaaatactg
    42961 ccaagaatac gaatcagcag ccccattacg ccaatgtagt acagcaagct gataagcgcg
    43021 gtaacgaaaa taatcgccgg gagtacgcga aaggcgaaga taaagcccgc accgtcaaac
    43081 agaacatcca ttttgggacc gaccagcgaa ccaaaaataa aggcacttcc tgcgtcactg
    43141 taggacatca ctttatggac gcctaatgcc gcctgctcta ctgcccattt tcccggggga
    43201 aaatagagca tgatgccacc aatggcaatt tgcagcagca gcgcagcgcc aaccgtgcgc
    43261 aagctgatgc tctttttatt taccgacaac acaaaggcaa ttgccagcaa taccaccatc
    43321 cccgcaacac ttctcattat atccataatg attttccctt catgccagta aacccggcgt
    43381 cagcgccagg ttttggtatg tttgatgagt acgggcgacg gctttctgcc cgccagaaat
    43441 taacccacga gacgctgata ttctttggca atttcgctcg ccagaatggc gttattaaag
    43501 accagttgaa tattggattt caggctatca ccgccggtta gctccgcaac acgcgccagc
    43561 aggaacggtg tactctcttt cccaataaca ccctgttctt cggcttcagc aacggcttga
    43621 tcaattgctg cattgatggt ttcttctggc atcgcaaact gttccgggat cgggttcgct
    43681 accaccaggc cgccgttcaa accggtttgc cacttcactg ccatcgcgcg agcaatttcg
    43741 cgggcactgt caagacgaat gctgacttca aatgggctgg ttcggcagaa aaacgcaggc
    43801 agcgcttttg tctgataacc aattaacggt acaccgaagg tttctaaata ttctgttgtt
    43861 aatcctaaat cgagaataga ttttgccccc gcacaaacaa cagtgacatt ggtatttgcc
    43921 agttcctgta aatcggcaga aatatcgaag gtatgttccg ctccgcgatg cacaccacca
    43981 atacctccgg ttgcaaaaac tttaatccca gccagtgctg caataatcat ggttgatgca
    44041 acggtagtcg cgccattttt ccctgcagca acgacaaaag gtaaatcacg acgactaact
    44101 ttcgtcacgt tatgtccttc acggcccagc aattcgattt cttctttact taagcccact
    44161 ttcatcacac cgccaataat ggcgatagtg gctggtactg cgccctgttt acgaattgtt
    44221 tcttctactt caattgcggt ttgggcattt tgtgggaatg gcattccgtg agaaattatg
    44281 gtcgattcca gcgcaacaac cggtttttta ttttttaaag catcctgtac ttcagcggat
    44341 atttgtaata attcagggga cagagttaat tcagacattc tgtgttctcc actaacgatc
    44401 taacattggc aattgataat tcagggttgt tggtatattc acaggcaagt gccattgacg
    44461 aacatccctg cgcaaatcga acagaatcga taaacggcat accgtctacc caacaggagg
    44521 ccagccccgc catcatggcg tcgcctgccc cagtaacatt gatgacattc gttttaatag
    44581 gggccgacca cccactttca ccgttgatat cactgtagta aacgccatcc cctcccatgc
    44641 tcagcaccaa gcggttcagg ccgtgttgat gaaaccaggc ggcgaccttt gcgacatctt
    44701 cacgtccgga aagtgcaatt ccgctcaacg tttcggcttc aagacggtta ggttttaacg
    44761 tatggatttt gctcagatga tcgcggattt tgacgcattt ccatgccgat accggatcaa
    44821 taaatacagg cgtttccccg ctattttcca gaacccagac cagcacctct tcactaaggt
    44881 tgcaatccgc gacaataacc tttgcaccgc gaataaactc ctgatgctgg gcaagaaatt
    44941 ctgcagaaat acagtcacta atactcatat catttatcgc gacaagcatt tctcctgtat
    45001 tatcgagcag tgataaatag ctggatgtat tctcacccgg aacaattaag catttatcga
    45061 cataaacacc ggattgatta gtttgcgcca gtaatgattg cccataaaaa tcaccaccta
    45121 ctgcacttaa aagccaggca tttttcccca gtaacgccag attatgagcg atattccttc
    45181 ccacgccacc tggggtaaat ttaatttttc cgggattcga atccgcataa ttcaatggat
    45241 gatgtgaata cccagccaca tcaatattcg ccgaaccaat aattactatg tagtcctttt
    45301 cgcgcatagc acgccccctg gcaataaatt gccaacgaag aaattgggta aaatagccat
    45361 tacagtcaat aagactatta gagcacgtgt tcagatttaa acacatgttc ataataagca
    45421 tatttaacgt aatgtaaatc gatcagaaaa aacgaaatcg gccagatatg aaggcggtca
    45481 caaagtttgt ctatttatgc agcaacaaaa gagaaagttg tgatgtgtaa cacacaatat
    45541 ggcaggcaag aaaaagggca gagatgtttc tgccctccag attgttgaca acgcgcgctt
    45601 tgttcatcct ggatgtggcg taaacgcctt atccgaccta caaaagcgca aaaaattcaa
    45661 tatattgcag ggttcatgta ggtctgataa gcatagcgca tcaggcaatt ttgcgtttgt
    45721 catcatcggg gcagagatgt ctcatccccg ataatgaaga taaattatgc gtttttttct
    45781 actatttgtg tttccgggcg tttcaggaag gcataggcca aacccgccac cagcgtaccg
    45841 gcaataattg ctaccaggta acccaatacc ggcgtaatag cgccagggat cagcagaaca
    45901 aacagaccac cgtgtggtgc catcagtttc gcaccaatcg ccattgagat tgcgccagtc
    45961 agcgccccac caacgataca gcacggtagc acacgcatcg gatcacgagc agcaaacgga
    46021 attgcacctt ccgaaataaa gcacagacca agtaccagag cggctttgcc accttcctgc
    46081 tgcgctttgt cgaatttgcg acgcgccacc attgttgcca gacccattgc cagcggtggc
    46141 accatacctg ccgccataat cgccgccatc gggccatagg tttgagtact cagcagaccc
    46201 acaccgaatg cgtacgctgc tttgtttacc ggaccgccca tgtcagtaca catcatgcca
    46261 ccgaggatcg cccccagcag aaccgcattc gcagtcccca tggtctgcag ccagtgagtc
    46321 aacccatcga gaatgccagc aaccggttta ccgattaggt agatcatcgc cagaccgacc
    46381 accagactgg aaattagcgg aatgatcagg atcggtttca gcgcctccat actctgtggc
    46441 agtttcaatt gcgtactgat taacttcgca atgtaaccag ccaggaagcc agcaattata
    46501 ccgccaataa agccagaacc ggtgctgacc gccagcatac cgccaatcag acccggtgtg
    46561 aggcccggac gatcggcaat ggagaaggca atataacctg ccagtaccgg caccatcagc
    46621 gcaaaggctg aaccaccacc aatctgcatc agcgccgctg ccaacgtacc cggctcttta
    46681 aacgcttcga taccaaaagc aaaagaaagc gcgatacaca gaccacctgc aacaaccatc
    46741 ggcagcatgt aagagacgcc cgtcagcaag tgacggtaag cgcctgcact ctctttctta
    46801 ccttcagtgg tcgctgtttg agctttgccc gctggttcat acggcgttgc ttcagcaacc
    46861 gctttatcca gttcctgcgc agttttcttc agcgccagac cggtagaggt gcgatacatc
    46921 ggtttaccag caaatttcgc cagatccact tcgatatctg ccgccacaat caccagatcc
    46981 gctgctgcga cttcttcagg ggtgattgca ttgcccgcgc caacagaacc acgggtttca
    47041 actttcaccc accagccacg ttttttcgct tcggtttcaa tggcttcagc cgccataaag
    47101 gtgtgtgcta cgccagtcgg gcaagcagtc accgcaacta cgcgtttcgg accgctggca
    47161 gcaaccggtg ctgtcgcagc gaccggcgca gtgtaaggtt tcgcatggcc tttggcttca
    47221 ctcaggaaca gctcagggtg cgcaactgcc cgggaaatat cacccagcca gacattttta
    47281 ccgttcagcg cgctgtcatt cgggatggaa tcaccgagaa caatcgccat ttctgcgtcg
    47341 ttcggattgt cgatgatttc cagttttgct tttcgcgccg ccgcgcccag cagggtcttc
    47401 gccatatagg cgcgtgcctg accgagatta gcgtcaataa tcagcagcgt tttcattatg
    47461 cctctcctgc tgtcagttaa aaggttgtaa gtcgacgcgc gccatcattg cggccaactg
    47521 cggacgatcg gtaataccca cattgctttg acttaccgcc agggctgcaa cagctgtcgc
    47581 cagacgcagt gtgtgttcac tggattcacg catcagcaag ccataaatca ggccaccaac
    47641 catagaatcc cctgcgccaa cggtgcttac gacatcgact gacggtggtt tggcgatcca
    47701 ttcgccggag gcattaaccc aaagcgcgcc ttcggcaccc agtgaaataa caacatgcgc
    47761 aataccttgt tcacgtagcg catgtgcagc ttcaatcaca tctttcattt caggcagttt
    47821 acggcctgcc cagatttcca actcgcggcg gttaggtttc accagccacg gtgccgcttt
    47881 caaacctgct actaacgctt cacggctact atcaaagata atgcaaggac actgactacg
    47941 caggcgagtc atccagtcgg tgaacgcttc cgggctgacg cctgacggta agcttccgct
    48001 gacacagacc atatcgaact gaccgagcca gctcagagaa tcagtcacaa agcgttccca
    48061 gtcggcgagg gtgacttcaa aacccgagaa gttgaagtcg gtcacttcgc cgtctttttc
    48121 cgtcagctta acgttaattc gggtgcgccc ctgtacaacc tggaaacggt tggcaatgcc
    48181 cagctcgctg aacagttgct gaaaaccatc ctgattgtct ttacccagga agccgccaac
    48241 ggtgacatca attcccaggt cttttaatac tttggccacg ttgatgcctt tacccgccgc
    48301 atgcagaccg gtggttttca ccaggttcac ttcgccgcgt tcaatttccg ggcagaaacc
    48361 aacaaggtca taagccggat taagggtgat cgtagcaaca cgtctgctca ttatgcgccc
    48421 tccccaagac cagcagcgat agcgtcgcca atggctttca gcgcctgttc agcatctgca
    48481 ccctgggcgg taaagcgtag gcgatgacct ttcttaacgc caagtgccac aactttcatc
    48541 agactacgtc cgtttgccgg tttgccggtg ccatcaaggt ttgtcacggt aatatcactg
    48601 ttaaattgtt taatggtatt gaccagcatg gtacctggac gagcatgcag gccgtgttca
    48661 ttgcgcacca caaactccgc gcttaacacg tcgtcggtcg gcgcatcatc gctggtcagc
    48721 agcgccagca acgttgccgc atccgctttc agcaagcggt cagctttatt gtcgagcaat
    48781 aaatcagcga gacgcttaag aaccgcgatg ggctgatcgt cattcatcgc cacactcacc
    48841 agcatggctg ccgtttcgcc gtccacatca aaagcatttg ccgcacggct taccgcaatc
    48901 gcgctacgca gattgccttc ggcgctatcg ctcagccaga taccctgtcc gagattcagc
    48961 ggttgttcat tgatggcttt ggtgacgaaa gtggcgtcaa ctgcccccac ctctttcaga
    49021 cgcgcagcgt tcagcgcctg aagagtcagc agatcgctgg cgacgatatc cagcgtcagc
    49081 atttcgttgt cgagcttcag ctgctcactc tgcttttcgc ccatcagtaa tgcgcgaagt
    49141 tcttctgctg ttgttgctga cttcagttgt tcagcaacgg aatcatcgct cagtacgtgg
    49201 gtcagctggc gtagcaggcc cagatgttca tccgagctgg cagcaatacc gattgccacg
    49261 tacgctacct gaccgtcacc ccaggtgacg ccttccggga actgaaatac ctgaacgccg
    49321 gttttcagca cctgatcgcg ggtgtcggta gtgccgtgtg gaatagcaat accattgccg
    49381 aggaacgttg aggtttgctg ttcgcgcgcc agcatgccat tgacgtagcc ttctgctaca
    49441 ttaccggcct gcaccagcgc cgcagcgacc tggcgaatcg cctcttcttt gtctccggcc
    49501 ttttcgcccg gatggatgtc ctgtacggat aactggaaca tagttctcct ctcttgctga
    49561 attgaaacga ttcagcctct atgagaaaaa aagcgccaac ctggcttagg gttaaagaca
    49621 agatcgcgct gaaacgtttc aagaaagcat aatacttctg tttcagcacg caaggaaagc
    49681 cgtaaagtta tgagcaaaag tttgatgtgc tgcacaattt tttgtcattt ttcctaattg
    49741 ctgatgggaa aatccgttat cagcaatttc atttcagcag cgtcagcagc caatcagcaa
    49801 cagtgaaatg gcattttgat tattcgtggc gcttttgatt tcgctgtctg tttattttct
    49861 gacaagcagc gtaaactccg cgtcttcctc ttccagtgat cgacctgcat gcataactcc
    49921 cccgcagtct ccagcgcgaa atcgtttgac ctgacctcga cggcgttttt aatcgttgcc
    49981 tttctcaccg gtattgcggg tgctctgcaa accccgacac tcagtatttt ccttaccgat
    50041 gaagtacatg cccgtccggc gatggtggga ttcttcttta ccggcagcgc tgtcattggg
    50101 attctggtaa gtcagtttct cgctgggcgc tctgataagc gcggcgatcg caaatcgctg
    50161 attgtctttt gctgcctgtt aggcgtgctg gcctgcaccc tttttgcctg gaatcgcaac
    50221 tactttgttt tgctattcgt tggcgtcttt cttagcagct ttggctcgac cgctaacccg
    50281 caaatgtttg cccttgcccg tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc
    50341 agctcttttt tacgcgctca ggtttcactg gcatgggtca ttggcccacc gctggcttat
    50401 gcgttagcga tgggtttcag ctttacggta atgtatctga gcgcagcggt agcgtttatt
    50461 gtttgcggcg tgatggtgtg gctgttttta ccgtcgatgc aaaaagagct tccgctggcg
    50521 accggcacgg ttgaagcgcc gcgccgtaac cgtcgcgata cgctactgct gtttgtcatt
    50581 tgtacattga tgtggggctc gaacagcctg tacatcatca acatgccgct atttattatc
    50641 aacgaactgc atcttcccga gaaactggcc gatgtgatga tggggaccgc cgccgggctg
    50701 gaaatcccga cgatgttgat tgccggatat ttcgccaaac gtctgggtaa gcgtttctta
    50761 atgcgcgttg ctgccgtggg tggcgtctgt ttttacgcag gaatgctgat ggcgcattca
    50821 cctgtcattc tgttgggctt gcagctgcta aatgctattt ttattggcat tctgggcggc
    50881 atcgggatgc tctattttca ggatctgatg cccggtcagg cgggttcagc caccacgctc
    50941 tataccaaca cttcgcgcgt gggctggatc atcgcaggat cagtggcggg catcgtcgcc
    51001 gaaatctgga attatcacgc tgtgttctgg tttgcgatgg tgatgattat cgccactctg
    51061 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
    51121 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
    51181 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
    51241 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
    51301 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
    51361 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
    51421 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
    51481 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
    51541 aacggcaaac tgctgctggt taaggatatt gatattcagt cgcccactgc ccgcggtgcc
    51601 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
    51661 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
    51721 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
    51781 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
    51841 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
    51901 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
    51961 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
    52021 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
    52081 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
    52141 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
    52201 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
    52261 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
    52321 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
    52381 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
    52441 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
    52501 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
    52561 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
    52621 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
    52681 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
    52741 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
    52801 cgccgccgtg agcgcggcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
    52861 aatggccatg tggtgaaaaa cgcggtgctg ggaatggcag aaaaatgttc gccagaactc
    52921 gccgggtgga taaaaaaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
    52981 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
    53041 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
    53101 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
    53161 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
    53221 ttcgtccata tcagtgattg catgcaggat cgcgtatttc gccatgctgc cagaacatta
    53281 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
    53341 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
    53401 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
    53461 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
    53521 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
    53581 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
    53641 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
    53701 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
    53761 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
    53821 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
    53881 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
    53941 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
    54001 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
    54061 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
    54121 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
    54181 gatctgttaa ccgcaccagt ctatgaactg tggatagatc tgcgcgccac cttgtgcatt
    54241 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
    54301 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
    54361 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
    54421 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
    54481 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
    54541 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
    54601 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
    54661 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
    54721 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
    54781 gagctgattt ccagcagcga agctgactgg aatgctttgc agagcgcgct gttgaagctt
    54841 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
    54901 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggcctcgcg ctgtttcttt
    54961 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
    55021 tctttaatca gaaactggtc gaaagtaagg cctttttgtg gctggttgca ttgaccaaca
    55081 atcgcgcttt cgacggttgt tctctgctgg cgatgggaat gttgatgctg agtttctggc
    55141 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
    55201 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
    55261 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
    55321 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
    55381 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
    55441 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
    55501 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
    55561 ccttttttga caaatcacta ccgggaaaaa acaaacattt ccaaaacaaa taactcacag
    55621 taattaacat catcaggctt atttttatag tgaggataat cctgatgatg cgcaccgtgc
    55681 tttcatccat cgcgcgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
    55741 ctcaatcgta ctttaattga ccaatcccgc ttattaactt tctgtatcac tttttcttat
    55801 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatcta gaactcgttt
    55861 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
    55921 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt
    55981 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
    56041 cgattttgag atatatcttg cgcgggatcc ccgcgattgc agtagcggtt ctgctttctg
    56101 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
    56161 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
    56221 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
    56281 gcagcactaa aaaaggtatc gattgttctg gttttgtaca acgtacattc cgtgagcaat
    56341 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
    56401 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
    56461 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
    56521 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
    56581 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
    56641 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
    56701 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
    56761 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
    56821 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc
    56881 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
    56941 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
    57001 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
    57061 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
    57121 tcaatgaatt gattccggcg ctcaacatta ataaaaacgt cgatatggcg atcttacccg
    57181 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
    57241 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
    57301 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
    57361 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
    57421 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
    57481 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctc tgctattacc
    57541 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
    57601 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgagtaacg ggcctggaag
    57661 tactgctacg ctggcggcat cctgtcgcgg gagaaattcc cccggatgcc ttcattaact
    57721 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
    57781 gcgatgccgc agaattagaa aaagtgctgc cagtaggcgt caaatttggt attaacattg
    57841 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
    57901 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
    57961 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
    58021 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
    58081 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
    58141 acgcggtgct gacgctggcg aaacgcctca atatgctgac ggttgctgaa ggggtcgaaa
    58201 cgccggaaca ggcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
    58261 ttagtcgccc tttaccgctg gacgattttg tccgctggct gaagaaaccg tatacgccgc
    58321 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
    58381 aatgatgagt aaaaggaaat ccattgcaga tgattgtgcg catactgctg ctgtttatcg
    58441 ctctgttcac ctttggtgtg caggcgcagg ctatcaagga aagctatgcc tttgccgtgc
    58501 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
    58561 caaaaggtgg gcagataacg ttgtcagccc tcggcacctt cgataatttc aaccgctatg
    58621 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgcta tttacgactt
    58681 ccgatgacga accaggcagt tattacccgc tgattgctga aagcgcacgc tatgctgacg
    58741 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttcgccca
    58801 ttactgcccg cgatgtagag tttacttttc aaaaatttat gaccgaaggc gtgccgcaat
    58861 ttcgtctggt ctacaaaggc accaccgtca aagccattgc accgttaacc gtgcgcattg
    58921 agttagctaa acccagcaaa gaagatatgc tgagtctgtt ttcgctgccg atatttccag
    58981 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
    59041 gtccgtaccg cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
    59101 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
    59161 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
    59221 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
    59281 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
    59341 cgtttaatat ccaacgtccg gtattcagcg atcgccgggt ccgggaagca atcactctcg
    59401 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
    59461 gttattttca gaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
    59521 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
    59581 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
    59641 acgaaacggg ctgggtgctg aagggtcagc aacgcgttaa tgctacaacg ggtcagccgc
    59701 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
    59761 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
    59821 ctaaccgcat gcgcagtcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
    59881 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
    59941 cccccggcgt gcaaagcccg gttatcgact cgctgatcaa ccaaattatt gccgcgcagg
    60001 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
    60061 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
    60121 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
    60181 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
    60241 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
    60301 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
    60361 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
    60421 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagac ccagaagtga
    60481 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
    60541 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
    60601 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
    60661 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
    60721 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
    60781 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
    60841 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
    60901 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
    60961 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
    61021 aaggggtaag tgagaaaaac attctctgga aacatgtgtt ccgcaacgcc atgctgctgg
    61081 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    61141 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    61201 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    61261 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    61321 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    61381 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    61441 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    61501 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    61561 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    61621 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    61681 ctggctggga acggatgcca acggcggcga tgtgctggca cgcattctct atggcacgcg
    61741 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    61801 gggggcgcta caaggctatt acggcggtaa agtcgatctc tggggacaac gctttattga
    61861 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    61921 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    61981 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    62041 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    62101 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    62161 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    62221 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    62281 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    62341 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    62401 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    62461 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    62521 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    62581 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    62641 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    62701 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    62761 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    62821 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    62881 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    62941 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    63001 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    63061 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    63121 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    63181 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    63241 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    63301 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    63361 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    63421 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    63481 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    63541 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    63601 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    63661 cattaattct taagccctca ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    63721 cggtacaggc gcaaatattg acgctattga aatcattgca acaaaagcat caactggcct
    63781 atttgtttat cagccacgat ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac
    63841 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc gcaccgcagc
    63901 aggagtatac gcgtcagcta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    63961 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    64021 tggcgattta caaacaggca cggctcacct tcacactcca gcaccgaaca tggaacttcg
    64081 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    64141 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    64201 tcaaccttcg aacctgcgaa accagcagac cagcgaaagt tctgcggtag acgatggacg
    64261 attgagagtt gtaatgaagt cacttatttt ccccggaagc acatttactt cacaatttat
    64321 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    64381 cctcgcctgc atggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    64441 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    64501 gactttttac ataaattgat tttacataaa ataaacatat atcgggggaa tgatagattt
    64561 gtggttgaca tgcatcaaca aaggaagcct tttagcttcc tcgttgtgca atagatcacc
    64621 gttttttcgg tcgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    64681 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    64741 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    64801 gaaactcatc aagaatgacc gccatcgcat tggatgacac catagacacg cagcccacaa
    64861 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca
    64921 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    64981 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    65041 caaagtaata accaaagttt tccggtgcaa cgtggttgat ttcaatgtag acaaacggtc
    65101 cggcgctcag gaacgagaac attccggcaa agctgaaacc gctggccagc atataactca
    65161 ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaaag
    65221 gctggcgagg atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac
    65281 tatcggtgcc atcagcggtg caatggtcgt caccagcatg acaaacgaca tcatccgcga
    65341 gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc
    65401 cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc
    65461 acacgccacc gcggcggcgg caaacaccag cgtaccgcca agaaccaccg gcttacgccc
    65521 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
    65581 actcagggtc atctgcgtac tgcccgccgg tacgccaaac tgcgctgaaa ttaccggtag
    65641 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
    65701 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa
    65761 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
    65821 atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc agctcaacca catggttacc
    65881 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
    65941 ctgcgttggg gtaatcactt ccagcaccgc cggcttagtg agatcttttt cgttatgcag
    66001 ctgcacgcct ttagcaaatt gttctgccgt atcgtcagct acgggtgata ccagcgtcac
    66061 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
    66121 atctgtcatt agtaccagac cggtggtatc gatatccagc cgccctgcgg cgtgcagttt
    66181 ccacgctacc ggttcatcta gaaaatagag caccgttggg tgatcagggt cgtccgtgga
    66241 gcaaacataa ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag
    66301 cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg cattacggac
    66361 gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
    66421 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
    66481 aaacggaggt cgggtaatga cccgaatatc tgaactgttg tctgcccagt atagcggtct
    66541 aataacatcc ctcaagggaa aaagatccgt ggcatactat tagcagaata atctacctac
    66601 gcgagaccat gatttttaca cttcgcccat atcagcaaga agccgtggat gccacgctca
    66661 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa
    66721 gcctggtgat agcggaactg gcacggctgg ctcgtggtcg cgtactggtg ctggcacacg
    66781 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
    66841 tttttgccgc cgggctgaag cgtaaagaga gccacggtaa agtggtattt ggcagtgtgc
    66901 agtcggtcgc ccgtaatatt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
    66961 aatgtcaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
    67021 caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca
    67081 aaggttggat ctaccagttc cattatcacg gcatggtacg cagcgatgag aaagcccttt
    67141 tccgtgactg catttatgag ctgcccctgc gttatatgat taaacacagc tatctgacgc
    67201 cgccagaacg actggatatg ccagtagtgc aatatgattt cagccgcttg caggcacaga
    67261 gcaacgggct gttcagcgaa gccgatctca accgtgagct gaaaaaacag caacgtatta
    67321 ccccgcacat catcagccag attatggagt tcgccgaaaa gcgtaaagga gtgatgattt
    67381 tcgccgcgac ggtcgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgcag
    67441 cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa aattttaaag
    67501 cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt gacgccccgc
    67561 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
    67621 tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg
    67681 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
    67741 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
    67801 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
    67861 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
    67921 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
    67981 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
    68041 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
    68101 atgaagacgg tgcggatgtg agtgagcgtt tccgtctgca aacacctgcc cagcgtaccg
    68161 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatcccg ctgcgctgga
    68221 tcaccgccgc cgatatcctc gcccagcaag ccttattgcg acccccggat tttgtcgtcg
    68281 cccgcatgaa aggccagtac tggcaggtgc gtgaaaaagt gttcgattac gaaggtcgtt
    68341 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
    68401 agtatagaat ctcacccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
    68461 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
    68521 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc
    68581 atctacggtg gcaaagaagc gccgctggct atcgagctgg atcacgacaa agtcatgaac
    68641 atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa
    68701 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
    68761 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccc gcggggtttt
    68821 ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg
    68881 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
    68941 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcagggtat cggtcgccgg
    69001 ctcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
    69061 gctaccggca aacttcgtca actgacgcag tgtgctccgg tctgccggga agctctcttc
    69121 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag
    69181 ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
    69241 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
    69301 gaaatcatca accgcttgca acagcccacg gttttgtgct ttcgtgttca atccttcgct
    69361 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa
    69421 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
    69481 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
    69541 acgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg ccagataacg
    69601 gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
    69661 caggcgacca gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa
    69721 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
    69781 gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa
    69841 tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
    69901 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
    69961 accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga
    70021 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
    70081 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg atgaacaggt
    70141 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
    70201 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
    70261 ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga
    70321 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
    70381 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
    70441 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc
    70501 gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact
    70561 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
    70621 ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
    70681 gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg
    70741 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
    70801 tagtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
    70861 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
    70921 gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
    70981 acccgatgac ggcgcgacgt tttcttgaga agcatggtct gcttgatgcg caggagtatc
    71041 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat ccgttaagcg
    71101 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
    71161 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
    71221 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
    71281 tctatggtat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
    71341 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
    71401 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
    71461 aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccactac gcacaagaag
    71521 ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc
    71581 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg
    71641 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggttgtgatt atcactgccg
    71701 gtcggggtat tccgctgagc gaagacgaag aaacctttga ctggtcccac ggtcatctgc
    71761 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gcgctgactg
    71821 atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc acacctgcca
    71881 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
    71941 cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
    72001 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
    72061 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
    72121 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc
    72181 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
    72241 gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccttt acggttggtt
    72301 tttttatatc tgcaattaat ttgataaaca gaccgtgaca catcacagcc tgtttatttt
    72361 ctgttatcag aacgtccaga ccactcccgc ctgagtattc cacggcgatt ctacccctgc
    72421 gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat
    72481 cccagcctga taaacgccac gggttccagc cacatcatta ttaaatttgc catcgtcatt
    72541 aactttcaca tggttagaat cggcgtattc ctgacgcacg gcggctttca gccagggttc
    72601 caacgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg
    72661 taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc
    72721 atctgtggta aagccggtaa aggcgagata gggtctaaca ctccacaatc cgtcaaccca
    72781 acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt
    72841 tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc
    72901 atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg tgtagctatc
    72961 aatattgcct ttgccgccgc gatcaaaacc aatatcagaa tgagagtaat caaagaacaa
    73021 gccgcgaatt gtgctgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc
    73081 cgtcaatgtt tgctcaaagc cagctcccgc atcagtggtc acgttgttgc gggtgttaat
    73141 tgccgaactc cacatcgccg tatcgtaatt aacgcctttt acgctaccaa gacgctcacg
    73201 cacggtgtcc agttctgcat caaataccag cggttgtgct gctgccatgt tcagcacatc
    73261 agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt
    73321 atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt
    73381 gaatgcagca tcaccgccgc ccgtcgttac cagtgtaagg ctatctcctg ctgccgggct
    73441 ggcccccgtg tccgtcacga atattttgaa atcacctgtt gcctgaccga tgacgttgag
    73501 ctgatcgctc tgatgattag ccatatcggt acgcatataa aattgccgtt tcccgacagg
    73561 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
    73621 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
    73681 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
    73741 acgtccgctt ggctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
    73801 actaccttac ccaccaacat agcgggtgca taatttattt tcggcttcat gacctcaaga
    73861 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
    73921 ccattgttga taaccatatt ttcagagatg gctttagagg aatacgtgat acttccattc
    73981 tgatctgatc gaccgagatc atatacagca tttgcctgca ctttcgtacc agtatccttt
    74041 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg agtgtcctcc
    74101 ataacaatga gcccggagcc atcatccagt tcataatttt ttgacacacc atcctttata
    74161 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
    74221 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaat
    74281 cctccctttt cgatagcggc aaagacggca atcccatctt tttcaacaat taagcgcccg
    74341 cctttaatcg tgggatcatt cgccgc
//
LOCUS       DAJTVK010000005        70303 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000005 DAJTVK010000000
VERSION     DAJTVK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.005"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..675)
                     /locus_tag="OO372_000362"
     CDS             complement(<1..675)
                     /locus_tag="OO372_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCR6339970.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHF"
     gene            complement(697..2709)
                     /gene="hyfR"
                     /locus_tag="OO372_000363"
     CDS             complement(697..2709)
                     /gene="hyfR"
                     /locus_tag="OO372_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6339971.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2739..3152)
                     /gene="hyfJ"
                     /locus_tag="OO372_000364"
     CDS             complement(2739..3152)
                     /gene="hyfJ"
                     /locus_tag="OO372_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6339972.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3145..3903)
                     /gene="hyfI"
                     /locus_tag="OO372_000365"
     CDS             complement(3145..3903)
                     /gene="hyfI"
                     /locus_tag="OO372_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6339973.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3900..4439)
                     /gene="hyfH"
                     /locus_tag="OO372_000366"
     CDS             complement(3900..4439)
                     /gene="hyfH"
                     /locus_tag="OO372_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6339974.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4449..6164)
                     /gene="hyfG"
                     /locus_tag="OO372_000367"
     CDS             complement(4449..6164)
                     /gene="hyfG"
                     /locus_tag="OO372_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR6339975.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6154..7734)
                     /gene="hyfF"
                     /locus_tag="OO372_000368"
     CDS             complement(6154..7734)
                     /gene="hyfF"
                     /locus_tag="OO372_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6339976.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7739..8389)
                     /gene="hyfE"
                     /locus_tag="OO372_000369"
     CDS             complement(7739..8389)
                     /gene="hyfE"
                     /locus_tag="OO372_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6339977.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8401..9753)
                     /gene="hyfD"
                     /locus_tag="OO372_000370"
     CDS             complement(8401..9753)
                     /gene="hyfD"
                     /locus_tag="OO372_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6339978.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9729..9839)
                     /locus_tag="OO372_000371"
     CDS             complement(9729..9839)
                     /locus_tag="OO372_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR6339979.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9856..10803)
                     /gene="hyfC"
                     /locus_tag="OO372_000372"
     CDS             complement(9856..10803)
                     /gene="hyfC"
                     /locus_tag="OO372_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6339980.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GALITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10814..12832)
                     /gene="hyfB"
                     /locus_tag="OO372_000373"
     CDS             complement(10814..12832)
                     /gene="hyfB"
                     /locus_tag="OO372_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HCR6339981.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12832..13449)
                     /gene="hyfA"
                     /locus_tag="OO372_000374"
     CDS             complement(12832..13449)
                     /gene="hyfA"
                     /locus_tag="OO372_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6339982.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13702..14172)
                     /gene="bcp"
                     /locus_tag="OO372_000375"
     CDS             complement(13702..14172)
                     /gene="bcp"
                     /locus_tag="OO372_000375"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6339983.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14172..14744)
                     /locus_tag="OO372_000376"
     CDS             complement(14172..14744)
                     /locus_tag="OO372_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6339984.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14890..15768
                     /gene="dapA"
                     /locus_tag="OO372_000377"
     CDS             14890..15768
                     /gene="dapA"
                     /locus_tag="OO372_000377"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6339985.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15785..16819
                     /gene="bamC"
                     /locus_tag="OO372_000378"
     CDS             15785..16819
                     /gene="bamC"
                     /locus_tag="OO372_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6339986.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17032..17745
                     /gene="purC"
                     /locus_tag="OO372_000379"
     CDS             17032..17745
                     /gene="purC"
                     /locus_tag="OO372_000379"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6339987.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17913..18761
                     /locus_tag="OO372_000380"
     CDS             17913..18761
                     /locus_tag="OO372_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6339988.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18776..20791
                     /gene="tmcA"
                     /locus_tag="OO372_000381"
     CDS             18776..20791
                     /gene="tmcA"
                     /locus_tag="OO372_000381"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6339989.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20865..21563
                     /gene="ypfH"
                     /locus_tag="OO372_000382"
     CDS             20865..21563
                     /gene="ypfH"
                     /locus_tag="OO372_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6339990.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21673..21873)
                     /locus_tag="OO372_000383"
     CDS             complement(21673..21873)
                     /locus_tag="OO372_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6339991.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21901..23028)
                     /gene="dapE"
                     /locus_tag="OO372_000384"
     CDS             complement(21901..23028)
                     /gene="dapE"
                     /locus_tag="OO372_000384"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6339992.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23032..23388)
                     /locus_tag="OO372_000385"
     CDS             complement(23032..23388)
                     /locus_tag="OO372_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6339993.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23770..23829
                     /gene="ypfM"
                     /locus_tag="OO372_000386"
     CDS             23770..23829
                     /gene="ypfM"
                     /locus_tag="OO372_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6339994.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23928..27041)
                     /gene="acrD"
                     /locus_tag="OO372_000387"
     CDS             complement(23928..27041)
                     /gene="acrD"
                     /locus_tag="OO372_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6339995.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27205..28905)
                     /gene="narQ"
                     /locus_tag="OO372_000388"
     CDS             complement(27205..28905)
                     /gene="narQ"
                     /locus_tag="OO372_000388"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR6339996.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29111..31090
                     /gene="aegA"
                     /locus_tag="OO372_000389"
     CDS             29111..31090
                     /gene="aegA"
                     /locus_tag="OO372_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6339997.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31158..31733
                     /gene="nudK"
                     /locus_tag="OO372_000390"
     CDS             31158..31733
                     /gene="nudK"
                     /locus_tag="OO372_000390"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6339998.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31859..32902
                     /locus_tag="OO372_000391"
     CDS             31859..32902
                     /locus_tag="OO372_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6339999.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32998..35001)
                     /gene="tkt"
                     /locus_tag="OO372_000392"
     CDS             complement(32998..35001)
                     /gene="tkt"
                     /locus_tag="OO372_000392"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6340000.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35021..35971)
                     /gene="tal"
                     /locus_tag="OO372_000393"
     CDS             complement(35021..35971)
                     /gene="tal"
                     /locus_tag="OO372_000393"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6340001.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36260..38539
                     /gene="maeB"
                     /locus_tag="OO372_000394"
     CDS             36260..38539
                     /gene="maeB"
                     /locus_tag="OO372_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6340002.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38832..39167
                     /gene="eutS"
                     /locus_tag="OO372_000395"
     CDS             38832..39167
                     /gene="eutS"
                     /locus_tag="OO372_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6340003.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39180..39659
                     /gene="eutP"
                     /locus_tag="OO372_000396"
     CDS             39180..39659
                     /gene="eutP"
                     /locus_tag="OO372_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6340004.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39634..40335
                     /gene="eutQ"
                     /locus_tag="OO372_000397"
     CDS             39634..40335
                     /gene="eutQ"
                     /locus_tag="OO372_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR6340005.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40332..41135
                     /gene="eutT"
                     /locus_tag="OO372_000398"
     CDS             40332..41135
                     /gene="eutT"
                     /locus_tag="OO372_000398"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR6340006.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41132..42148
                     /gene="pta"
                     /locus_tag="OO372_000399"
     CDS             41132..42148
                     /gene="pta"
                     /locus_tag="OO372_000399"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6340007.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42187..42480
                     /gene="eutM"
                     /locus_tag="OO372_000400"
     CDS             42187..42480
                     /gene="eutM"
                     /locus_tag="OO372_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR6340008.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42587..42874
                     /gene="eutN"
                     /locus_tag="OO372_000401"
     CDS             42587..42874
                     /gene="eutN"
                     /locus_tag="OO372_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR6340009.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42886..44289
                     /locus_tag="OO372_000402"
     CDS             42886..44289
                     /locus_tag="OO372_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR6340010.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44300..45136
                     /gene="eutJ"
                     /locus_tag="OO372_000403"
     CDS             44300..45136
                     /gene="eutJ"
                     /locus_tag="OO372_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR6340011.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45126..46313
                     /gene="eutG"
                     /locus_tag="OO372_000404"
     CDS             45126..46313
                     /gene="eutG"
                     /locus_tag="OO372_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCR6340012.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46630..47856
                     /gene="eutH"
                     /locus_tag="OO372_000405"
     CDS             46630..47856
                     /gene="eutH"
                     /locus_tag="OO372_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCR6340013.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     CIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47853..49256
                     /gene="eutA"
                     /locus_tag="OO372_000406"
     CDS             47853..49256
                     /gene="eutA"
                     /locus_tag="OO372_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6340014.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49268..50629
                     /gene="eutB"
                     /locus_tag="OO372_000407"
                     /pseudo
     CDS             49268..50629
                     /gene="eutB"
                     /locus_tag="OO372_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50650..51537
                     /gene="eutC"
                     /locus_tag="OO372_000408"
     CDS             50650..51537
                     /gene="eutC"
                     /locus_tag="OO372_000408"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCR6340015.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51547..52206
                     /gene="eutL"
                     /locus_tag="OO372_000409"
     CDS             51547..52206
                     /gene="eutL"
                     /locus_tag="OO372_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6340016.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52219..52719
                     /gene="eutK"
                     /locus_tag="OO372_000410"
     CDS             52219..52719
                     /gene="eutK"
                     /locus_tag="OO372_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6340017.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52765..53817
                     /gene="eutR"
                     /locus_tag="OO372_000411"
     CDS             52765..53817
                     /gene="eutR"
                     /locus_tag="OO372_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR6340018.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="OO372_000412"
     CDS             complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="OO372_000412"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6340019.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="OO372_000413"
     CDS             complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="OO372_000413"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6340020.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55809..56234
                     /locus_tag="OO372_000414"
     CDS             55809..56234
                     /locus_tag="OO372_000414"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6340021.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56221..56670
                     /locus_tag="OO372_000415"
     CDS             56221..56670
                     /locus_tag="OO372_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6340022.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56731..57306
                     /locus_tag="OO372_000416"
     CDS             56731..57306
                     /locus_tag="OO372_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6340023.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57402..58301
                     /gene="yfeX"
                     /locus_tag="OO372_000417"
     CDS             57402..58301
                     /gene="yfeX"
                     /locus_tag="OO372_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6340024.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="OO372_000418"
     CDS             complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="OO372_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCR6340025.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59668..59841)
                     /locus_tag="OO372_000419"
     CDS             complement(59668..59841)
                     /locus_tag="OO372_000419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HCR6340026.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59854..61092)
                     /gene="murP"
                     /locus_tag="OO372_000420"
                     /pseudo
     CDS             complement(59854..61092)
                     /gene="murP"
                     /locus_tag="OO372_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="OO372_000421"
     CDS             complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="OO372_000421"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6340027.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62156..63013
                     /gene="murR"
                     /locus_tag="OO372_000422"
     CDS             62156..63013
                     /gene="murR"
                     /locus_tag="OO372_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6340028.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63142..63933
                     /gene="ucpA"
                     /locus_tag="OO372_000423"
     CDS             63142..63933
                     /gene="ucpA"
                     /locus_tag="OO372_000423"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6340029.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64104..65120
                     /gene="cysP"
                     /locus_tag="OO372_000424"
     CDS             64104..65120
                     /gene="cysP"
                     /locus_tag="OO372_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6340030.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65120..65953
                     /gene="cysT"
                     /locus_tag="OO372_000425"
     CDS             65120..65953
                     /gene="cysT"
                     /locus_tag="OO372_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6340031.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65953..66828
                     /gene="cysW"
                     /locus_tag="OO372_000426"
     CDS             65953..66828
                     /gene="cysW"
                     /locus_tag="OO372_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6340032.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66818..67915
                     /gene="cysA"
                     /locus_tag="OO372_000427"
     CDS             66818..67915
                     /gene="cysA"
                     /locus_tag="OO372_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6340033.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68049..68960
                     /gene="cysM"
                     /locus_tag="OO372_000428"
     CDS             68049..68960
                     /gene="cysM"
                     /locus_tag="OO372_000428"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6340034.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68963..69331)
                     /locus_tag="OO372_000429"
     CDS             complement(68963..69331)
                     /locus_tag="OO372_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6340035.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69436..70287
                     /gene="pdxK"
                     /locus_tag="OO372_000430"
                     /gene_synonym="thiJ"
     CDS             69436..70287
                     /gene="pdxK"
                     /locus_tag="OO372_000430"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6340036.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16700 a        18348 c        19437 g        15818 t
ORIGIN      
        1 gaaatggcga atggcaatgg cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa
       61 cccactggcg acaaacaggg tgatgggcaa aatcatggcg acgattttgt cgcataatga
      121 acgcccgcaa taactcatcc acagcgccag acaaaccatc agattgcaca taatgccgag
      181 gctgacagat tcagtaaatg tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac
      241 tccccacatc gcgttttcac tcatcaccag cccggaaaac cagattaaca aactgaaaca
      301 ggcaatacct gccagattac cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca
      361 actaataacg ccccgacttt ttgccatcac cgtcattacc gacgaggtga acaggctggt
      421 gccacaaacc gccagcaaaa taaagccgag tgtaaagcaa aggccgccca ccagatgggt
      481 taatgcctgt gaagacgggg catcggcgat aacggaaagg taaaaagtaa atccaatcgc
      541 cataaacacg ccagcggaca tcgccattaa aaagaccgac actttgctgc gggcaatttt
      601 atgggcagta atccgttcag cgattgccgc ttttctggcg ctcaactgca agtcgaaaga
      661 gagtttgttt ctcatgcata ctcctgcaaa agcagattac aacacctcgc gaaccgagag
      721 ccccagacgc tgcattcgtg acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc
      781 gccacggggc ccggcaacaa tgccattggt ttctcgcaat acctgaacaa tgcgctgacg
      841 ttcttcttcg tcattctctg gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag
      901 cgctgaatct tcatttagcg tcggcagtaa atggctttgt cggacattaa gatgtaaatt
      961 cagactgtta ccgcgagtca gcagtaccgc tcgctcaatc acgttttcca gctcgcgcac
     1021 gttgcccggc cagtcccacg acatcaactg gcgtagcgcc tcggtcggga tggcgtcaat
     1081 tgaacgattc atatggcgcg ccattttttg cgtgaaatgc tttgctaaaa gagggatatc
     1141 ttccggacgg tcgcgtagcg gcggcaattc cagtgggaag acattcaggc gataaaagag
     1201 atcgctgcga aactggcgat cttcaaccat ttgccacaaa tcacggttgg tggcggcaat
     1261 gactctgaca ttcaccggga tcgttctact cccgccgaga cgctcaatct cccgctcctg
     1321 caatacgcgc agcagtttag gctgaagttc taacggcaga tcgccaattt catcaagaaa
     1381 caacgtgccg ccatcggcaa tttcaaaacg accacgatgg gtattaatcg caccagtaaa
     1441 cgcccctttg tcatgaccga ataactcact ttccagaaga ctggcgggga tggcagcgca
     1501 gttgattttg accagcggct tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat
     1561 cacctctttg ccggttccgg tttcaccaca aatcaacact gtactgtcgc tcttcgccac
     1621 aatatctacc tgctggagca ggtcttccat tgcctggctt tgatagataa tgtcaccgac
     1681 gcccagattc gaaaggagct gctcgctaag ctggtggttt tcttgctgca aactttcctg
     1741 caaatcggtc atgctacgcc aggcatcggc attgccaacg gcaatagcga tgcgatcggc
     1801 tatgtgttgt agtagctgac agttttcctc actaaagaga gtggaagagg tatgcgccag
     1861 caacaatgca cccggtgtat ggttgccaaa ggtaagcggt ataaggagcg cagattcgca
     1921 gccgttagat gcaagtaaga gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc
     1981 acggtgcgtc aaggtcggtt gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc
     2041 aggcatattg cgtggcagac acgcacaatg tgaggcagaa agatcgctgc accacaggct
     2101 aaacttctcg ttctttcgat gatcgcccag taccatactg actgaagcca gaccgaaaaa
     2161 atgatggatc tcacgagcga cgtcagcgat cagatcgtcg agatcaagat gtgacagcac
     2221 tgcattagta atatcaacca gtacccgatg atgatcgcga tctttctcca gcaaacgcag
     2281 cgccacctgc tgttcttcat cattgagcca tgattgcaga accggggtga ttatccgcgc
     2341 cagccagtta aacagtgtgc aatcctgggg agtaaaacgg cagccgtggg tgcgaataaa
     2401 gcgtagctcc gccattccac tactgccgag aaggacggcg tgacgcgaat aatcgaccat
     2461 ctcgccctga tactctgttg ttgccgtcac atcgctgcca tcatcatggt agcggtaata
     2521 gaagttgctt ttgagaacat gcagttcaat aagttgtacc gggctgaacg gcggcggcag
     2581 cgggatgaag gcgcgtaata aagacgcctt accgtgtttc tgcgctaacc gctccgcgag
     2641 cattccgttt accgcttcaa ttgttattcc ttgcggcggg gcaaacatcg cctcgtctga
     2701 catagccatc aacagtcctt attaatgaga aatccttctc acgcaatttt tctggccatc
     2761 agatagatgg ccggttcctg cacaatttca tccaacagat gaatgaattg aatgctacgc
     2821 gcctgccaca cagacgtcgc gtcatccgcc agtggcgcga atgcccgggt caacagggcg
     2881 gtgtggctgg cgtcaataac aatctcacca aaagtcatca cccccagcat cttacgtcgg
     2941 gcttgctcct cttcgaccag tgcaagccaa tcttcgtatt ccgtcagtgg gcaggggaag
     3001 gcgacattca gacaatcaat cacgccaacg tgatgaccga tagccagcga gtaatacatt
     3061 acctgagagc tgtgttccgg catctcgtcg ctactggcga caaacttttt tcgcagcgtc
     3121 cagaaaacaa tttccccgca ctcttcagtc atgtaacccc cggagtacgc gaaagagttg
     3181 ctgaacgata ctattgagac gtggatcgtc ggcatcgcgc aaccaggtgt taacccgatt
     3241 tcctgtagga tcgtcgctta aatggcgcag gagccgatcg caaatttctc gcccctgacg
     3301 atagcccgcc agccgccgcg cttctcgctc aatggcgata cgctgtgatg gcgggatctg
     3361 cggccacaac ggttgcatag tcaccccggt gggatcgcga taatccaccg cgtgaatctt
     3421 ctgttgcagc aaaccgagcg ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg
     3481 gcagccgggg atccaaacat caatggggac aatggtgtcg ctgccgcccc agacgctgta
     3541 gagatcgtgg aaaataccgc cgccgacacc gcacgcgccg taggaaacac aaattttatg
     3601 atcgggggca gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc
     3661 tgtaaataac aaaatatcgg cgtgacgcgg tgatgaaata accttaatgc caaaacgttc
     3721 tgcgtcgaat actggtgtga tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc
     3781 gcaatcgacg cgataaacgt aagccgagcg acggatatcc tgtagcaaat gctgcttcat
     3841 cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca tgttgtgtaa gcactggact
     3901 catagctgct ccttagccac cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt
     3961 tgtttgcact ccgggcagac gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca
     4021 ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg
     4081 cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag atcggcttta
     4141 ttggtgacgg tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct
     4201 tcacaacgtc cgcagtaaat acaacgcctc agatagagct gccaggtgcg cgaattttgc
     4261 tggtcgtcgg tctggatagt cagcgcattt gccggacaag cacaggcgcc gcaggcaata
     4321 cattggctgg gcatcaggtc cggtttcccg cgaaagcctg ggctgacctc cagtggcgcg
     4381 aagggatatt tcaccgtcgc ggttccggcg cgcataatag ttttcagtaa cttcagcatc
     4441 ttctgtcctt acttcagcgg cgaacggtta cgatcaatgc cgtagcgttc gatctcttta
     4501 tacggcacgg tttttgactg gcgcttgcgc acatctacca gcgtcacacg gtcggtacag
     4561 gagtagcagg gatcaaggct accgataatc agcggtgcgt cagaaacggt attgccgcgc
     4621 agcatgtaac gcaacaccgg ccagttggcg taggtggcgg cacggcagcg ccagcggaac
     4681 aatttttggt tatcaccgag catgctccag tgcacgtctt caccgcgtgg cgcttcaaca
     4741 aagcccagcg cgaatgcgtg aggtttatag ctaaagcctt cggtcagcag tggggtatcc
     4801 ggcatgttgt cgagggcaaa ttccagcatt gccagcgaat caaacgtctc tttgacacgg
     4861 accatcacgc gggagaaaac atcgccgccg gtaaaggtaa acagtgtttt tggaatattg
     4921 ccgtagtcgg cgtaggggtg atcaaagcgc aaatcacggg caaaaccact gccgcggatc
     4981 agcggcccta cagggctata atcacgggcg atttgtcggt cgagaatgcc aatgccctga
     5041 gtgcgttgtt ccatattcgg cgtggcaagc agcatctcta ccagctccga cacgtcggcg
     5101 cgcatctcgc gcaccagttt cagcgtttgc agacgttgct ctttgagaat atcgcggcga
     5161 acaccaccaa tcagattcag accgtaggtt ttacgcgacc cggtcagcaa ttccgccatt
     5221 gtcatcgact tttcccgcac gcggaaaaat tgcataaagc cggtatcaaa tccaacaaaa
     5281 tggcaggaga ggccaaggtt gagcaaatga ctgtgcagac gttcgacttc cagcagaatc
     5341 gagcgaatag tatgcgctcg ttgcggcacc tcaatcccca gtgcattttc aaccgagttg
     5401 gtataggcca cactgtgggc aaaaccgcaa atcccacaca cgcgatccga taagaaggtc
     5461 acttcgttgt agcccatccg cgtttctgcc agtttctcca tgccgcgatg gacataaaac
     5521 aggcggtaat cagcatcgac aatttgctcg ccatccacaa acaagcggaa gtgacccggt
     5581 tcatcggagg tgatatgcag cgggccaata gggatcaccc gcgcatcgct attgccctca
     5641 ttgataaacg gatacgtttc ggtatcagtc gtcggttcag ggcgcagtcg ataatccatc
     5701 gcatctttgc gcagcggatg catatcttcc ggccagttat cgggcaacac caggcgacgc
     5761 tgatccggca ggccaaccgg aatcagcccg tacatatcgc gaatttctcg ctcgccccag
     5821 accgcggcag ggacgcgcgg tgtgactgac ggaaactccc gactgtcggc atcgaccagt
     5881 gccttcacca cgatccagca tttttcggcc ccttccattg aaagggcata ataaaccgcg
     5941 taatgaccgt taagtgtccg ctcgtcgttg ccaaacagga ctggaagcca gccatcatgt
     6001 tgataataaa gataatgtac aacgtcaggc agcagattga ttttcaccgt aatggtgacc
     6061 tgttcaggcg tttgtcgctc ttcatccagc accgcgccgg ggaactgcgt tttcagggcg
     6121 gcgagaatcg cttcgccacg atttgacgat gaattaacgt tcactgtgtt tctccgaaac
     6181 agatgcggtg cctgaaggca aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg
     6241 cgtccctgag aggactatag tggaagcgcc cgccaggatc ctgatgacag gttgtggaat
     6301 atgcgttccc atcgccagca tcatgaccag cagaatcacc attggcgagg tggtcaacca
     6361 gccgagatca ccccggttaa cggcctgcgg cggtttcgcc attaacaccc gcgctgccat
     6421 ccgtaccagg cccgccagca ccagcgttaa cagcaataac agcaggacga taatcagcag
     6481 gtgattacgt gccagtccgg cggtaatggt cataaattcg ctaagaaaaa tgttgaaggg
     6541 cggcatccct gccagcgcca gcgcaccgcc gccaaacagc acggcggtaa atggcatgat
     6601 tttgagcatc ccacagacga cgttgagatc gcgcgtgccg tacttgagca gtacattgcc
     6661 ggaaccgcag aacagcagcg tttttgccag actgtggttt aagatgtgca gcagcgcggc
     6721 aaaaattccc agcggcccgc caatgcctag cgccaccgcg accagcccca tgttctccac
     6781 gctggagtac gccagcagac gcttaatgtc ccgctgtacc agaatgaaaa atgccgccac
     6841 ggcaaccgac aacatgccga agatgagcaa caaccggttg gggaaatcgc tgccgatggc
     6901 ttggcaaata atgatgtaat agcgaatcag caccaacagc gcgcagttca gcaatacggc
     6961 ggagagcagg gcgctgaccg gactcggcgc ttcactgtga gcatccggca gccaggcgtg
     7021 catgggaaat agcccggttt tggtaccaaa gccaattagc acaaacacaa aggccaacag
     7081 cattaatgtt gggtcaagca aggacgattg cttaagaacc tcgctccaga atatcgccat
     7141 ttctgcctgc ggcataacgc tggcggcgtt ggcgtatacc agcacggtac cgaacagacc
     7201 aaaagcgaca ccaacagtac aaataatgat gtacttccat gctgattcca gcgatgaacg
     7261 ctgaccgtaa atgcctacca gaaacgccga gcttaaggtg gtggcttcga tcgccgccca
     7321 catcacaatc aggttattgc tggtaacaac cagcagcatg gtgaacaaaa acagatggaa
     7381 gaaaccgtag taatcgcaca gcgtaacggg tgaaagctcg ccgtgtgcca cttcatgacg
     7441 catgtagcca atcgagtaaa caccggtgag aaagccaatc acaccaagaa tggcgaggaa
     7501 caaaccgccc agaccatcaa tatgaagcca cagtcccgca gcgaatattt ctcctgcatc
     7561 agcggcagtt tggaccaccc agagtgccag aatcagcagc agtgtgatcc ctaagctatg
     7621 taatacagtc accgtacaag tcgcagagag cccccgtttc cggcaggcaa aacagagcag
     7681 cgaaaaaagc agcggcgtga gcaggagtaa agcgaacatc acagaataac tcatctcatt
     7741 atcccttcag cgcggtcaag ttgttcacgt ccagcgtgcc gtgggtacgc catatttttc
     7801 ttgccagtaa caccatcaca atgacggcga agatggcgtc ggtagcgata cctatttcca
     7861 ccagttccgg tgctcgccag gcaagaagcg ccagcaccag atgggagccg ttttccatca
     7921 ggcagtaacc aaaaatttgc cgcaggatat tgcgctggct gacaatgcac agcaggccaa
     7981 gcagaaaatg acctaacgct accgccagcg ccggtttcag cccggtagcc atcggtagct
     8041 tcacgggctg aacgacaaat gcgcaaagca ggacaattaa cgccgcgagc agtgccatca
     8101 ttgccggacc gaataacgct ttttccggga tgttctgggg aatatttcgt gcagcgtaag
     8161 tcatgattaa cggtaccagc agcactttgg tgataaaggc gctggcggac cagatcagca
     8221 gttgctctgc ggcgaacagg cacgagagag tggcgaaaat agacaccagc accagcgact
     8281 ggcaggcgta aaatccgcat gacaggcgat agcttttgac gctaatcaca aacagcgatg
     8341 tcagcatcat cagtcccgcc agattattta cgatcataga accggtcatt catcatctcc
     8401 ttactgcaac caggtcgcgg cgattacgct ggaaatcagc gacatcacaa tcagtacaat
     8461 caacaccagg cgcattgacc ctggcagcgg tgcggcatcg gcgacggcct cgctcggttt
     8521 gcccggcacg acgcgaccaa accagcgaat aaaccaggcg aaactggcga ccgattcaat
     8581 catcagcaga atcatggcgg gcagcaggat ccagtactcc actgacaacg caaaaccgac
     8641 ggcaaacagc gggaatttac tgaagaagcc gttgaacggc ggcacaccgg taatcgccag
     8701 cgctgccacg cagaaaccca cgcttggcaa cggcagggtg tgcaatacgc cacgcagacg
     8761 cggcaacaag cgcgtgccgc agctgtaact cagcgcacct gctacaagga aaaacaggct
     8821 tttagcgaac gcgtggttga cgatgtaggc gatgctaccc tccagcgcca gccgcgagcc
     8881 gaagatggag agcgacaagc cgaagaacat ccagccaagt tgagtgatgg tcgaccaggc
     8941 cagcaaccgc ttcatatcct gctgtggcaa atacatcaga aagccataaa gaatggtgac
     9001 cagcgccatg accatgccaa cgccgccaat cacatgcggg atattgccgc cgtcgataat
     9061 ggcgcgggca aaaatgtaaa cgcccacttt caccatcgat gcggcgtgga gataggcact
     9121 gatcggtgtt ggcgcttcca ttgcatccgg tagccacgct tgcatcggta gctgggccga
     9181 tttcccccac gcggcaaaca ggatgccgcc ataaaccaga taacgtgcgt cgccgtgtaa
     9241 ctcgctcatc gcgctaagcg caaacgttcc ggtctgcaaa aacagcgtgg cggcggcaag
     9301 atacaacccc aacgagccga tatgagtgat aagtaacgct tttagtgctg aaagctgtgc
     9361 tttatcgctc tggtaataac tgatcaacgc ccaggagcag ccgcccgtaa tttcaaaaaa
     9421 caacaactga ccgagcagcg tcgaggagag taccagtccc gccatcgcgc cgataaacac
     9481 cagcaaaaat gcgtaataac gattcgtgcc gttatgcggg tgttcgcgat ttttatccgt
     9541 cagataaccc gtggagtaga tcgtgaccag caaaccgaga aacaccacca caaacagaat
     9601 cagcgtactc atgcgatcaa tgaccaagcc aaacaatgcc acatccccca cgttgaccaa
     9661 cgtcaacgtg acggcaactt tatcggcctg ataaaacgcg gagatcagcg acaacatgca
     9721 cagcgtggtc agtgcggcga acaaaacccc cattcggcgg cccgacgttg tggcgaaaac
     9781 gaaacgacca gtgcgccgat aaaaggcagc attaacgtcg tcagagcaag attttccata
     9841 ttccgtcagt gctccttaca gaccggttaa ccagaagacc catgcaagcg cagcaaggct
     9901 gaagccaagc caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc
     9961 tgcgattgag gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt
    10021 cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa
    10081 cagtgctgcc atcacgacct gtttcagccc cagcccccat ttcgctagcg ccagcccggc
    10141 accggaatat tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa
    10201 gggaattttt cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt
    10261 ggttagcggc gagttccagc ccgttgccag cgtattgctg atcatctcga tatgcgtgga
    10321 acctgctatc agcgccaata ccagcagtga gagaatgagc atgggttcga ccagaatgcc
    10381 gagcgtcaac tcgcgactgg caccgactcc cgcaaacggg cttccggtat ccagccctga
    10441 aagagcaaag aaaaaacgaa acagggcaag aagatagata agggtgatca gagcgccgcc
    10501 gcccgcaaaa ggggaaacgg taataaatag tggtagggcc atcgccagca ccagcatgct
    10561 gctgattaat acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcatcttc
    10621 ctggcgttta aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg
    10681 ggagtgcata cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag
    10741 cagaatcacg gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct
    10801 catggtgatt tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat
    10861 acaggcaata gagacgaaag tcgccgcttt gcagatgctg tatttctttg gccagccgtt
    10921 gggtggcgct cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag
    10981 ccctggcggt gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc
    11041 gtagacgaaa tagcgcagaa aagaccacac gcagcggctg catcacgcca ttgcctgacg
    11101 gggccatcgc attttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg
    11161 ctgcgcggcg cgaacgacac cacagccagt aaaggccagg cagcaaaggc atcgccagta
    11221 gtaacagcaa cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta
    11281 ttccgctggc gacagtgacg ggaggggtat tggtaaacgc atgggcaatg tgcattatct
    11341 tcggtgccag ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca
    11401 tggcgacgat catcgtattt ggcacttcct gtgcctcttc agcgtgtgtg ctgcgcggcg
    11461 caccacagaa agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga
    11521 ctgccagcat taccatagca ataggacccg caagttgtag cgctacggct tccacacgac
    11581 ttagtgagaa tagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa
    11641 tggctgatat cgccaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg
    11701 ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcacccgcgc
    11761 cgagaaatag cagccctttg aaaagcgcat ggttgagcag atgaaacagt gcgccgagca
    11821 gtccaaccac ggtgagcagc gggtcgtgca gtgacagacc gaccatcgcc acaccgaccg
    11881 ccagcaaaat aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct
    11941 gttccgccag tgcatacagc acgcccagga gcgcggagat tgcgccgatc gccatcacca
    12001 gaatgcccca ccacagcggc aaacccgttt gcgccagcag atccatcgcc actttcagga
    12061 tgccgaaaat accgattttg accattacgc cagacatcaa cgctgaagcg tgcgatggtg
    12121 cggcagggtg agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa
    12181 aaccgaaaaa ggccagcagg aacaccgccg acgccagccc cggagaaagt gaaagcgtgc
    12241 ggaaactggc aaaatcgagg ctgccgcttt cgcgccccat cagcaagaag gcgatcatta
    12301 tcagcaccga tccggcgtgg gcgataaaaa agtagagcat gccagcgttg atcgacgttt
    12361 tatcctgcct ggcaatgacc agaaaccagg aagacagcga catcatttca aacagcacga
    12421 tgaaccaaaa agcgttgtcc atcaccagca gggcaaccat cgatgcgatg aaaatattca
    12481 taaagaagcc catcgccgcc gcgcctttgc cctcgtattc gcgcatataa gtcaatgaat
    12541 agagagaaca aaccaccacc agcaaggaga taaccagcac cataaatgcc gagaggctat
    12601 ccattcgcag ggttaaatcg gcaaattcaa aaggcgtggc aaaacgggcg actaaagtta
    12661 cgcctgcatg taattgcgta attccgctga tgatgccaat cacgccgccc accagcgatg
    12721 tgatgccgga aagcttaata gccagtctgt ccagaccgag taaaaacagc gaagccagac
    12781 tggcaaacag atagaggatc agcgaccagg ttaataattg cagggcatcc attagcgttg
    12841 ctcctgagtg agggaaaggg gatcctcccc gccattggca aaccagcttg cggcaaggcg
    12901 ctgtttttct ttcatctggc gttgcaggct atcgtcggtg atcagccgta acgcctgatt
    12961 cgggcaagcg cgaacgcagg ccggaccttc tggcaagaaa tcacacaggt cgcatttcac
    13021 cgcgacggtc tggacacccg gttcccagcg cagcaacgca tgttggggcg gcacgttttc
    13081 ttcgccgtct ttgagtgagc cctcagcctg aaaaacatat tgcgcagggg cattcaccgg
    13141 acggcttcct gaagcgctga ttgcgccaaa tgggcagacc acggcgcaaa gcttgcagcc
    13201 aatacagagg ctttcgttga gttggatcgc atcatccctc tgagagatgg cattgaccgg
    13261 gcagacctgc aggcaagggg cttcctcaca gtgatgacac acgacagggg cagtgagtgt
    13321 tgacgtcttc gccagggcca ggcgcgggtg ttgctgtaaa ccttgcgttt tatgcacgtc
    13381 cgaacaggca gcgagacagg tattacatcc tgtacaccac agtggttcgg ccaccacaaa
    13441 gcggttcatg tcggctcctt gaaaatgata tgaaacattg catttcatat tgcataaagg
    13501 atgctattct gattaactca atatattcaa taagttatgt attaactgga ttctgaacgg
    13561 tacaaaaatg atggtcatta acgttcagca aaaaatggat tcgacacccg tgtcgcaggg
    13621 gaaagcgaga atgtgatctt gatcacgaga agtgaggtat gctggccgca agcgcagcca
    13681 gcacggaatg gagcaaagta atcaggcgtg ttctttcagc cagttcagca caacgtcgtg
    13741 gtgattgctg gttttgaaat cgtcaaagac atgttcgatt ttgccatcag cgtcaatcag
    13801 gaagctgatg cgatgaatgc catcgtaggt tttgcccatg aaggactttt caccccagac
    13861 gccgaattgt tcgcacacct ggtggtcctc atcagacagg agcgtaaagt taagcagctc
    13921 tttttccgca aaacgggaga gtttttcggg tttatcggtg ctgataccca gcacatcaac
    13981 gcccgctttt ttcaactcat ccatgttatc gcgtaagccg caggcctgta cggtacagcc
    14041 gggggtcatg gctttcgggt agaaataaac cagaacacgc tgtccctgga agtcggtcaa
    14101 attaacttgt tctccgtctt gatccggcaa gctaaatttc ggtgcgatat caccggcttt
    14161 cagtggattc attacttaac tccatcctgt tcatcatgtt gggaataatt gacgacgtta
    14221 atactgcctt gtgcattgag ttctgtacat agggctttga acgcttgctc aatatttgct
    14281 gcgtccgcag atgcggggct gtgggcggtt atctgaatat gcaactgcgc agcccgttca
    14341 ttttcagcag gttgcgtgcg cgacaccagc tccgcaatgt tcatatgatg cgcgtcgaaa
    14401 agtgctgtga agcgttcaat taaatgcggg gagtctgcca catcgacctg aacccagaca
    14461 gatgctggca ttggcggacg cggacgcgcc gtcgtgcgct tcatcacgat taaaagatcc
    14521 agctcggcac ctttcaacgg taacgttgat tcaatcagag taatggcatt ccatgaaccg
    14581 gaaagcagca tgataaacgt gaactcttct cccagcatcg ccaggcgact gtcttcaata
    14641 ttacagccgc aactactgac atgacgggtg atggtgttca caattccagg gcgatcggca
    14701 cccaatgcag tgatcaccag ataatgttgc gatgacagtg tcaaactggt tattccttta
    14761 aggggtgagt tgttcttaag gaaagcataa aaaaaacatg catacaacaa tcagaacggt
    14821 tctgtctgct tgcttttaat gccataccaa acgtaccatt gagacacttg tttgcacaga
    14881 ggatggccca tgttcacggg aagtattgtc gcgattgtta ctccgatgga tgaaaaaggt
    14941 aatgtctgtc gggctagctt gaaaaaactg attgattatc atgtcgccag cggtacttcg
    15001 gcgatcgttt ctgttggcac cactggcgag tccgctacct taaatcatga cgaacatgct
    15061 gatgtggtga tgatgacgct ggagctggct gatgggcgca ttccggtaat tgccgggacc
    15121 ggcgctaacg ctactgcgga agccattagc ctgacgcagc gcttcaatga cagtggtatc
    15181 gtcggctgcc tgacggtaac cccttactac aatcgtccgt cgcaagaagg tttgtatcag
    15241 catttcaaag ccatcgctga gcatactgac ctgccgcaaa ttctgtataa tgtgccgtcc
    15301 cgtactggct gcgatctgct cccggaaacg gtgggccgtc tggcgaaagt aaaaaatatt
    15361 atcggaatca aagaggcaac agggaactta acgcgtgtaa accagatcaa agagctggtt
    15421 tcagatgatt ttgttctgct gagcggcgat gatgcgagcg cgctggactt catgcaatta
    15481 ggcggtcatg gggttatttc cgttacggct aacgtcgcag cgcgtgatat ggcccagatg
    15541 tgcaaactgg cagcagaagg gcattttgcc gaggcacgcg ttattaatca gcgtctgatg
    15601 ccattacaca acaaactatt tgtcgaaccc aatccaatcc cggtgaaatg ggcatgtaag
    15661 gaactgggtc ttgtggcgac cgatacgctg cgcctgccaa tgacaccaat caccgacagt
    15721 ggtcgtgaga cggtcagagc ggcgcttaag catgccggtt tgctgtaaag tttagggaga
    15781 tttgatggct tactctgttc aaaagtcgcg cctggcaaag gttgcgggtg tttcgcttgt
    15841 tttattactc gctgcctgta gttctgactc acgctataag cgtcaggtca gtggtgatga
    15901 agcctacctg gaagcggcac cgcttgcgga gcttcatgcc ccggctggaa tgattttgcc
    15961 ggtgacctcc ggtgattatg caatcccggt gaccaacggt agtggtgctg tcggtaaggc
    16021 gctggacatt cgtccaccag cccagccgct ggcactggtt tctggcgcgc gtacccagtt
    16081 cacgggcgat accgcttcat tgctggtgga aaatggtcgt ggcaatactc tgtggccgca
    16141 ggtggttagc gtgctgcagg cgaaaaacta caccatcacc caacgtgatg atgctggtca
    16201 gacactgacc accgattggg tacaatggaa ccgtctggac gaagacgagc agtatcgtgg
    16261 tcgttatcaa atctctgtta agccgcaggg ttatcagcag gcggttacgg ttaaactgtt
    16321 gaacctggaa caggcgggca aaccggttgc agacgcggct tccatgcagc gttacagcac
    16381 ggagatgatg aacgttattt ccgccggtct ggataaatct gccactgacg ccgcgaacgc
    16441 tgcgcaaaat cgtgcctcca ccactatgga cgtacaaagt gcagctgatg acaccggttt
    16501 accaatgctg gtcgtacgcg ggccgttcaa tgtggtttgg caacgtctgc cagcggcgct
    16561 ggaaaaagtg ggcatgaaag tgaccgacag cacccgttcg cagggcaaca tggccgtaac
    16621 ttataagccg ctgtctgaca gcgactggca ggaactgggc gcgagcgatc caggcctggc
    16681 atccggtgac tataaactgc aggttggcga tttagataac cgcagcagcc tacagttcat
    16741 cgatccgaaa ggtcatactc tgactcagag tcagaacgac gcgctggtag ctgtcttcca
    16801 ggctgcgttt agcaagtaaa aatacagggc tggaatcatc cggccctttt ttctgatatg
    16861 atacgcaaac gtgtgcgtcg gcaggaaaac gcgattttag cggtaattcg cacgaaattt
    16921 gtttgtcgga cgtagttcgg ataaggcgtt cacgccgcat ccgacaaaac atccggcaca
    16981 ccagacagca aaagatttta aaacgttaat tcacacccag gagtgataaa gatgcaaaag
    17041 caagctgagt tgtatcgtgg taaagcgaaa accgtataca gcacggaaaa cccggacctg
    17101 ttggtgctcg aattccgcaa tgatacgtca gcaggggatg gcgcgcgcat tgagcagttt
    17161 gatcgcaaag gtatgttgaa caacaagttc aactacttca ttatgagcaa actggctgaa
    17221 gcgggtatcc cgactcaaat ggagcgtctg ctctccgata ccgaatgtct ggtgaaaaag
    17281 ctggatatgg tgccggttga gtgtgtcgtg cgtaaccgtg ctgcgggctc tctggtgaaa
    17341 cgtcttggaa tcgaagaagg tattgagctg aacccgccgc tgttcgatct gttcctgaaa
    17401 aacgatgcca tgcacgatcc gatggtcaac gaatcttact gcgaaacctt tggctgggtg
    17461 agcaaagaga acctggcgcg tatgaaagag ctgacctaca aagcgaacga cgtgctgaaa
    17521 aaactgttcg atgatgctgg tctgattctg gtcgacttca agctggagtt tggtctgtac
    17581 aaaggtgaag tggtactggg cgatgagttc tccccggacg gcagccgcct gtgggacaaa
    17641 gaaacactgg agaaaatgga caaagaccgt ttccgccaga gcctcggtgg cctgatcgaa
    17701 gcctatgaag ccgtcgcccg ccgcctgggt gtacagctgg actgattttt ctgttcatca
    17761 tcttgccgtg cagctggcac ggcaagacaa ccgctctcgg aatatatgca ttgaacgctc
    17821 attcctccgc catttcatcc cgctttcctg tggtaatctt cctctgaacc acctacgatc
    17881 atttctataa tgaatatatt gttgaggtat ctatgcgttg gcaagggcga cgtgaaagtg
    17941 acaatgtcga agacaggcgt aacagctccg gcggtccgtc aatgggcggt cccggttttc
    18001 gcctgccaag cggtaaaggc gggctgattt tactgatagt cgtgctggtt gcaggctact
    18061 atggtgttga tttaaccggg ttgatgaccg gacagccggt ttcccaacag caatcaacac
    18121 ggtcaattag cccaaatgaa gacgaagccg caaaattcac ctcggtgatt ctggcaacca
    18181 cggaagacac ctggggacag caattcgaga agatgggtaa aacctatcag caaccgaagc
    18241 tggtcatgta tcgtggaatg acgcgtaccg gctgcggggc gggccagtcc attatgggac
    18301 cgttctattg cccggcggat ggcacggttt atatcgatct ctccttctat gatgacatga
    18361 aagacaaact cggcgcggat ggcgattttg cccaggggta cgttatcgcc catgaagtcg
    18421 gtcatcatgt gcagaaattg ttaggcatcg agccgaaagt tcgtcaactg caacaaaacg
    18481 cgacgcaggc ggaagtaaac cgcttatctg tgcgtatgga actccaggcc gactgttttg
    18541 ccggtgtctg ggggcataac atgcagcagc aaggcgatct ggaagaggcg ctgaacgcgg
    18601 cgcaggccat cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca
    18661 gtttcactca tggtacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg
    18721 gcgatccggc acaatgcaat acttttggta aaagcattta acttccgggg caaggatggc
    18781 tgaactgact gcgcttcaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt
    18841 gctggtgttg agcggggaag agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc
    18901 cttacctggc gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttctcc
    18961 ctcggcgcta caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca
    19021 cggctttgat gccgctgcct ttgcggcact tagcggaacg ttgaaagcgg gaagctggct
    19081 ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg
    19141 ctggagtgat tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt
    19201 acttacggcg gataacgacg ctattctctg gcggcaaaac cagccgttct cgttggcgca
    19261 ttttaccccc cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca
    19321 actcttacag cagctactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg
    19381 gcgcggtaag tccgcgctgg cagggcaact catttctcgt attgcgggca gtgcgattgt
    19441 caccgcgccc gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg
    19501 ctttattgcg ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga
    19561 tgaagccgca gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac
    19621 gttgttaacc actacggtgc agggctacga aggtaccgga cgtggttttt tgctgaaatt
    19681 ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa cagccgatcc gctgggcaca
    19741 gggatgcccg ctggaaaaaa tggttagtga ggcactggtt tttaacgatg aaaacttcac
    19801 ccatacacca caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga
    19861 tccagaaacg ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacctcgcc
    19921 gctggattta cgccggatga tggatgcacc agggcaacat tttttacagg cggctggcga
    19981 aaacgagatt gccggggcgt tgtggctggt ggatgagggc ggattatctc aagaactcag
    20041 tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc
    20101 ggcgcacggc agcaatccac tggcggcgac attgcgcgga cggcgggtca gccggattgc
    20161 agtccatccg gcgcgtcagc gcgaaggcgt tgggcaacag ctcattgcca gcgctttgca
    20221 atataggcct ggcctcgact atctttcggt gagttttggt tacaccgggg agttatggcg
    20281 tttctggcaa cgctgcggtt ttgtgctggt gcgaatgggt aatcatcgtg aagccagcag
    20341 cggttgctat acggcgatgg cactgttacc gatgagtgat gcgggtaaac agctggctga
    20401 acgtgagcat taccgtttac gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaac
    20461 gcttcctgtt gatccactaa acaatgccgt cctttctgac gacgactggc ttgaactggc
    20521 cggttttgct ttcgctcatc gtccgctatt aacgtcgtta ggttgcttat tgcgtctgct
    20581 acaaaccagt gaactggcat taccggcgct gcgtgggcgt ttacagaaaa acaccagtga
    20641 cgcgcagtta tgtaccacac ttaaactttc aggccgcaag atgttactgg ttcgccagcg
    20701 ggaagaggcc gcgcaggcgc tgttcgcact taatgaggtt cgcactgagc gtctgcgcga
    20761 tcgcataacg caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt
    20821 gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt
    20881 ttgttgttca aagcccggat aaacctgcgc aacagttgtt actgcttttc cacggtgtcg
    20941 gggataaccc ggtggcaatg ggggaaatag gcagctggtt tgcgccgcta tttcctgatg
    21001 cgctggtggt gagtgtcgga ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt
    21061 tttcggtgca gggtattacg gaagataatc gccaggcgcg tgtggatgcc atcatgccga
    21121 cgtttattga gatggtgcgc tactggcaga aacagagcgg agtaggggct aacgccacag
    21181 cgctaatcgg tttctctcaa ggcgcgatta tggcgctgga gagcattaaa gccgaaccgg
    21241 gccttgcttc acgcgtcatt gcttttaacg gacgctatgc gagcctgccg gaaactgcgt
    21301 ctacagccac gacaattcac ctgattcatg gcggtgaaga tccggttatc gatctggcgc
    21361 atgcggttgc ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg
    21421 tggaggattt aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc
    21481 gttataccat tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg
    21541 atgatgtgat tgaaatgatg taaaagacag attacctggc ctgataagac gcgtgagcgt
    21601 cgcatcaggc atcgttcacc attgccggat gcggcgttaa cgccttatcc ggcctacggt
    21661 tgagcactca gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca
    21721 cttatcgttg aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttgtg
    21781 gtcgacgcgc ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac
    21841 cagaatccac caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca
    21901 tcaggcgacg agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc
    21961 agcgttcaca cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac
    22021 ctgcgccccc atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg
    22081 cggtttaatt tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg
    22141 cgcggtcaaa aatggctgcc cggaaagcca ccaatccacc gtatagcgca gttgatgttt
    22201 ttcaagcagg gcaagcacct gcgctttgat catctcatca gtcagttcgg tgctgaagcg
    22261 gaagttaaac tgcacaaaca gttcacccgg aataacgttg ttactgcccg ttcccgcctg
    22321 aatattggca atctgcatac tggtcgccgg gaagaattca ttgccctgat cccactcaat
    22381 agccactaat tcattaagga aaggtgctgc gcgatgtact ggattgtcag ccagatgtgg
    22441 gtaggccaca tgcccctgca cgccatgaat ggtaaggttg caggttaacg atccgcgacg
    22501 accatttttc accacatcac caaccacttc gatactcgac ggttcgccga ccagacagta
    22561 atcgagacgc tcattacgcg ccattaacgc ttcgacgact tttaccgtac cgttgtgggc
    22621 actggcttct tcatcagagg tgatcagaaa tgccagtcgt cccgtatggt tgggatgttg
    22681 tgcgacaaaa cgttctgctg ctaccaccat cgccgccagc gagcctttca tatctgccgc
    22741 accgcgcccg aataacatgc catcacgaat ggtgggttca aacggcggat tgatccaacg
    22801 atcggcgtcg ccaggcggca ccacgtcggt atgcccggca aaggccaatg tttcaccttg
    22861 cccacgccat gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc
    22921 gatcgcctgc aaacgatcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga
    22981 agggcggcga ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc
    23041 atggaaaaat tgctgataac tggaatcact gaaacccagc agcataggct taccgggcgc
    23101 gcagagcaat ggacgtttga taattgcagg catttcagtc attaatgccg ccgctgaggc
    23161 cgcatcggtg attttattgc gggtggtttc gtccagttta cgccaggttg taccacgggt
    23221 gttgagtaac gcctcccaac ctaattcgtt gataaaaccg ttcaataatt cgctgtccag
    23281 cccatcgacg cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg
    23341 agccttttta atggtgtcac aatttttgat accgtaaagt gtaaccataa tgaatccttt
    23401 ttcgatttat gagaataaat actcatttaa gggtgaaaac attttgcctg caaatgatta
    23461 tcaggaaata taacattatc gaagtattca aattaacaac tattaaataa tattaaacat
    23521 ttttaacgta aacattaaga atattcatct tgttatctgt ctgtatttta acctgaatta
    23581 aatcagtatt agatgccgta tgaatatctc gaaatgttgc tgtgtatcac atacaaatca
    23641 tcaccgcagg accatagtat ttacataaga tttgtccccc cggaggtagt catcgcagca
    23701 atcgagtaaa ttagcttcac cacggtatat tgtttccgta gaatacccat aattataaga
    23761 gaggttgtta tgattgaacg tgaactgggg aactggaaag actttatcga agttatgctt
    23821 cgtaagtaat tcctgagaca aagtataaac agcaagaacc cgctgagcag gttcttaatc
    23881 ggaaagttga aaaaggcgac acattggcat gtcgcctttt ttattgctta ttccgggcgc
    23941 ggctttagcg ggaagcggcg gcgcaccagc acaaagaaca gcggcacgaa gtaaatagcc
    24001 agaatagtgg ctgaaatcat cccgcccatt acgccagtac ccaccgcatg ctgaccaccg
    24061 gaaccggcac ccgtgctggt tgccattggc aatacgccga agataaatgc cagcgaggtc
    24121 atcagaatcg ggcgtaaacg ctgacggcag gcgtggagcg tcgcttcaaa caggtcgtgg
    24181 cctttttggt tcatctcgtt agcaaactcg acgatcagga tggcgttttt cgccgataaa
    24241 ccaatgaccg ttaacaggcc cacctggaag taaacgtcgt tttccagccc gcgcatccag
    24301 gttgccagca gcgcgccgat tacccccagc ggcacgacca gcattaccga gaacggcacc
    24361 gaccagcttt catacaacgc agccagacac aggaatacca ccagcaagga aatggcgtac
    24421 agcgccggag cctgcgcgcc ggaaagtcgc tcctgatacg acatcgccgt ccactccaga
    24481 ccaaagccgt ttggcagctg cttcactaac gattccataa tatccatcgc cgtaccggta
    24541 ctgacccccg gtgcggcttc cccaacaatc tcaaccgcag aataaccgtt atagcgttcc
    24601 agacgcggcg agcctgtttc ccagcgtgag gtcgcgaaag cagagaaggg caccatgccg
    24661 ccatctttat ttcggacata ccagagattg atgtcatctg gcagcatgcg atacggcgca
    24721 gctgcctgca catagacttt cttcacgcga ccgcgatcca taaagtcatt cacatagctc
    24781 gaaccccagg cggtttgcag tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga
    24841 gctttacgct ggtcgatatc aatctgcaac tgcggactgt cgtcgaggcc gttatggcgc
    24901 acacgggtta gctccgggtt ttccgccgcc agcgccagca actggttacg tgctgccatc
    24961 agcgcatcgt gacctgctcc agcgtggtcc tgcaactcca tatcaaaacc cgcagaacta
    25021 ccaagaccgc taattgctgg cgggctgctg gcgataacgc gagcttcttt aatcttgtta
    25081 aacgcctttg ttgcacgctc gataatggca aacgaggtgc cggtcttact gtcgcgttcg
    25141 ctccagtctt tcaggcggat aaacattcgc gccacgtttt gcccgttacc cccagggcca
    25201 gaaccaacgg tggcaaacac cgacatgatg ttgtcttttt catgggtgaa gtaatatttc
    25261 tcgatttgct caacgacttt cagggtctgt tgttgggttg aaccgctggg caattgtacc
    25321 gaggtagtaa acatgccacg gtcttccagc ggtaagaacg acgtcggcaa acgcaggaac
    25381 aggaacacca tgccgccaag cagcaggaca taaatcacaa tccagcgcag gctgcggtgg
    25441 agaattttcg ccaccccttt ttcgtagcgt tcggcgttgc ggttaaacat ctggttaaac
    25501 caggcaaaaa agcctttttg cccgtgatgc tcaccttttt tcagtggctt aagtagtgtg
    25561 gcacacagag ccggagtgag gatcatcgct accagtactg acagcaccat cgccgcaaca
    25621 atggtaatag agaactggcg atagatggca ccggtggtgc cgccgaagaa ggccattggt
    25681 acaaataccg ccgaaagcac catcgcaatc ccgaccagtg ccccctggat ctgccccatc
    25741 gatttacgtg tggcttcgcg aggagtgagt ccttcctcac tcataatacg ttcgacgttt
    25801 tccaccacca cgattgcgtc atccaccagc agaccgatcg ccagtaccat cgcgaacatg
    25861 gttaaggtgt tgacgctgta accgaaggcg taaagtacgg agaaggttcc catcaacacc
    25921 accggcacgg cgatagtggg tatcagcgtg gcgcggaagt tttgcaggaa cagatacata
    25981 acgaggaaaa ccagggcgat agcttccagc agcgttttca ccacgtcttc aatggaggct
    26041 ttaacaaacg aggtggtttc atacgccacc ttgtattcca gtccgtgcgg gaagtactgc
    26101 gccagctcgt cgagacgatt gagcaccagc tccgctgtcg ccatttcgtt tgcgccggag
    26161 gccagtttta cccccagccc ggaggctggc ttaccattga agcggctaag ataatcgtat
    26221 ttctccgccc ccatttcgac ggtggcgaca tcgcccagcc ttacctctga gccgtcttga
    26281 ttcacccgca aggtgatatc gcggaactgt tctggtgttt gcagcagtga ctgggcgtta
    26341 atggtggcgt tgagcgcctg cttatcgacg gaaggtgtac caccaagttg cccaaccgca
    26401 atctgcgcgt tctgtgactc aatggcatca gtgacatctt tagccgtcat ctggaaactg
    26461 ttgagtttcg ccgggtccag ccagatacgc atggaatatt gcgaaccata ggcatcgata
    26521 tccccgacgc cattcacgcg gcttaacggg tcctgaatat tactggcaac ataatcggca
    26581 atatcctgtt tatccatcga accatcggta gagacgaaag caatggtcag aatattggta
    26641 tcgccggttt tacgcaccgt cacgccctga ttttgcaccg cctgcggtaa ctttcgcatg
    26701 gctgattgca gctggttttg tacttgctgc acggcttcgt ccggatcggt gcctgcttta
    26761 aaacttaaag tgacagatgc ctgaccgatg ccactgctct gagatgacat atacatgaga
    26821 ttatcgaggc cggtcatatt ttgctcgata acctgggtca cggtgttttc cagcgtctgg
    26881 gccgatgcgc cgggatagtt agcggtcact cgcacattcg gtggcgcgag atcggggtat
    26941 tgttcaacgg gcaatgaaaa aatcgccagg gtccctgtca gacacaacag gattgccagc
    27001 acccaggcaa aaatggggcg atcaataaag aaattcgcca ttaaaagagg acctcgtgtt
    27061 tctgcgtatc gttgtaggct tgacttgctt cactgtagcg gcaaggtacg agccaatcgt
    27121 ggagaaaaaa aggagttaat gtaaattatc atgccctttt atactgaacc ttaagtgcaa
    27181 gtattctttg gtcagtagga ggcattacat caactgactt tcctcaccct ccgcagagcg
    27241 aaagctaata ctcactaacg tgccgccgcc ggaaggttgt gaaaaagtca gcgtcccacc
    27301 cagccgttcc gcgcgttcgc gcatgatatt cagaccataa tgaccttcgg gttctttcgg
    27361 ttcaccgata ccaatcccgt tatcacggat ataaaccgtg tgattgccgt ccggcgcggt
    27421 gacgcaactg acggcgattt cgctggcgtt ggcgtgcttc atcgcattca gcaccgcttc
    27481 gcgaataatt tgcaacaaat gcacctgcat ttgcgcatcc agcgccaggg ttggcagacg
    27541 gcagtcgagg gtcagtttgg cgctggtttg attttgtaac gtatccagca tttccctcaa
    27601 cgcggagggg agatccgcct gctgcagcgt caggcggaaa gtagtcaaca gctcgcgtaa
    27661 ctgccgataa gcatcattca acgcctggga aaagtcggcc atgatacttt gtgcggtggc
    27721 gttatcttcc ggtatcgaac gcttcagtaa cgtcaactgg atacgtaagt aagaaagtac
    27781 ctgagccagc gagtcgtgca attcgcgggc gatggttgca cgttcttcca tcaacaataa
    27841 ctgctgaaaa tgcttttgcg cctgattaaa gtacaaaccg cgtcccagca tcgacgaaac
    27901 gctgttaagc agcggttctg atgatgaaac gtgactattt tgccagtgca gttcgccgta
    27961 aaccgtctct tgcattgtca ccggtaaaat ctgcatcggc aattccgggt ttggttgccc
    28021 ttcgctaatc cgccagtttt caccgacatt taactccaga tattcagccg cttcattgtc
    28081 gcgaacaatc tgcaaaatat ggcggaaaca atgcacatca atctggcttg tgttcagcgc
    28141 ctgcgaacat tgatacaaca cctccagacg acgcttggcc tcgtggagat cgcgggtctt
    28201 ttcttctact gacgcctcca gcgaaaggta caatttatgc agctcgctcg acatctggtt
    28261 aaaggttttt gcaagcagac caagctcatt cggcagactg gtatccagcg gcggcgagtc
    28321 gaactgcccg tgttcaatac gttgactggc ggtaaccagc tgattcagcg gggcaaccac
    28381 ctgatggcgt atacggcgca aagtaaaaaa gaccagcgtg aaaataccga taccgccagc
    28441 cagggaaatc gccaccacca gcagcatttt gcgttcagcg tagtgctgta aagccagcac
    28501 gaacaggtct atctgattaa cataattatt aatattggcc tgataccacg gcaaatcgcc
    28561 cttgctgagc cgattattca tctccagcca gttggcattc agatgcgcat agcgagtttt
    28621 tactgcttct ggcacatacc agacgttgag attggttaat accgatgaat gcagtgcctg
    28681 ctgaaacaac tggcgatgtg cattgagttg tggactgcca ctttgcaagt cgtagcccag
    28741 gcggtaactc tgcatacgca gcgatccggc aatattgata gcctcagcgt cgcgcaaact
    28801 gctcgccaga gttagcagag cgatacccgt ggaaagaatc gacagcagca caatgtaaaa
    28861 aaaggcccgg gccagactgg ccgagacggg tcgtttaaca atcacacgcg tcttctccac
    28921 aaaaaatcat taatgttcca gttcgctaaa acgcaattta gtcagtgatg ggattatttg
    28981 atctgccaca gtttatagcg aaatagttac ttattctggg cacatgaaca ttgccgattc
    29041 ccctgtaagt cgggtaataa caacactcat ataaagaata aggtttttac aaccaaaaaa
    29101 gaaggtcgtt atgaatcgtt ttattatggc caacagtcag caatgtctgg gttgtcatgc
    29161 ttgtgaaatc gcctgtgtca tggctcacaa tgatgagcaa catgtcctga gccaacacca
    29221 ttttcatccc cgaattacgg ttatcaaaca tcagcagcaa cgtagtgcag tgacctgtca
    29281 ccattgtgaa gatgcgccct gcgcccgtag ctgccctaat ggcgcaatca gccacgttga
    29341 tgacagcatt caggtcaatc agcaaaagtg tattggctgt aaatcctgcg tggtggcctg
    29401 tccgtttggt acgatgcaaa tcgtcctgac acccgtcgcg gcaggaaaag taaaagccac
    29461 ggcgcataaa tgcgaccttt gtgcagggcg cgaaaacggt cctgcctgtg tcgagaattg
    29521 cccggcggac gcgctgcaac tggtcactga cgtcgcactc tccggcatgg cgaaatcccg
    29581 ccgcttgcgc accgcacgtc aggagcatca accgtggcat gccagtaccg ctgcacaaga
    29641 aatgccggta atgagtaaag tcgaacaaat gcaggcaacg cccgcgcgtg gcgagccgga
    29701 taaactggcg attgaagcgc gcaaaaccgg ttttgatgaa atttatctgc cattccgtgc
    29761 cgatcaggca caacgggaag cctcgcgctg ccttaaatgc ggcgagcaca gcgtttgtga
    29821 atggacctgc ccgctgcata accatattcc gcagtggatt gaactggtga aagccggaaa
    29881 catcgacgcc gccgtcgagc tttctcacct gaccaacacc ctgccggaaa ttaccggacg
    29941 cgtttgtccg caggaccgtt tgtgtgaagg tgcctgtact attcgcgatg agcacggcgc
    30001 ggtaactatc ggcaacattg aacgctacat ttcagatcag gcgttggcga aaggttggcg
    30061 tcctgactta agccatgtca ccaaagtgga caagcgggtg gcgattatcg gtgcgggtcc
    30121 ggcagggctg gcctgtgcgg atgttctgac ccgcaatggc gtggcggtga cggtgtacga
    30181 tcgccatcca gaaattggtg gcttgctcac tttcggcatt ccttctttca aactggataa
    30241 atccctgctg gcacgccgtc gggaaatctt cagcgcgatg gggattcact tcgaactcaa
    30301 ttgtgaagtg ggtaaagatg tctctttgga ttcgcttttg gaacaatacg acgcggtctt
    30361 cgttggcgta ggcacttacc gttccatgaa agcgggttta cccaatgaag atgcgccggg
    30421 cgtttatgac gcgctgccgt tcctcatcgc caacactaaa caggtgatgg gactcgaaga
    30481 gctaccggaa gagccgttta tcaataccgc cggactcaac gtcgtggtac tgggcggcgg
    30541 cgacaccgcg atggactgtg tgcgtaccgc gctgcgccac ggcgcgagta acgtcacctg
    30601 cgcttatcgt cgtgatgaag ctaacatgcc aggctcgaag aaagaggtga agaacgcccg
    30661 cgaagagggg gccaacttcg aatttaacgt ccagccggtg gcgcttgagc tgaatgagca
    30721 aggtcacgtt tgcggaattc gtttcctgcg cacgcgtctt ggcgagccgg atgcccaggg
    30781 gcgccggcga cttgtaccga ttgagggcag tgaatttgtc atgccagccg atgcggtgat
    30841 tatggtgttt ggcttcaatc cacacgggat gccgtggctg gagtcgcacg gtgtaacggt
    30901 agacaaatgg ggccgcatca tcgcggatgt ggaaagccag taccgttacc agaccaccaa
    30961 tccgaaaatc ttcgccggtg gcgacgccgt gcgtggtgcg gatctggtgg ttaccgcaat
    31021 ggcagaagga cgtcatgcgg cacaggggat tattgactgg ctgggggtaa aatcagtcaa
    31081 atctcactga tagcctgcgc agacaaaccc gacttcacag cgtaatataa ttgttcattt
    31141 cgcgctgtgg agtcggtatg acgcaacaaa tcaccctcat taaagacaaa attctctccg
    31201 ataactattt caccctgcac aacattactt acgatctcac gcgcaaagat ggcgaagtta
    31261 tccgccataa acgtgaagtt tacgatcgcg gtaatggtgc gacgatcctc ctgtacaacg
    31321 cgaagaaaaa gaccgtggtt ctgattcgtc agttccgtgt cgctacctgg gttaatggca
    31381 atgaaagcgg gcagctgatt gaaacctgcg ccgggctgct ggataacgac gaaccggaag
    31441 tgtgcattcg taaagaagcg attgaagaga cgggctatga agtgggcgaa gtgcgcaaat
    31501 tatttgaact gtatatgtcg cctggcggtg tgactgagct aatccacttt tttatcgccg
    31561 aatacagtga caatcagcgc gctaacgccg gtggcggtgt tgaagatgaa gatattgaag
    31621 tgcttgagct gccgttcagc caggcgctgg agatgatcaa aaccggcgag atacgtgacg
    31681 gtaagacggt gttattgctt aactatttgc aaacgtcaca tttaatggac tgaaaaataa
    31741 caataatatt tcgttgttta ttattggctc aatccgacaa atctgattga gccgcgctac
    31801 ctgcgcaacg aagatacgac ttgtgctgtt tgtttgaact tctggggtcg taccgtccat
    31861 gcgctatcgc attttccttc tctttttttt cgctttgttg ccgacgtctt tggtgtgggc
    31921 ggcactagcg caacgggcgt tttccgactg gcaggtcacc tgtaataacc aaaacttctg
    31981 cgtggcgcgc aatacgggcg atcataatgg actggtgatg accctgagcc gcagcgccgg
    32041 ggcgcatacc gatgccgttt tacgtattga gcgcggcggg ttgaagtcgc cggatgcgtc
    32101 tgaaggagag atagcgccac ggctgctgtt agatggtgag ccgttggcgt taagtggtga
    32161 caagtggcgg atttcaccat ggttattagt aaccgatgat acggcaacca tcaccgcgtt
    32221 tttgcagatg attcaggaag ggaaggcgat caccttacgc gacggcaatc agaccatttc
    32281 tctgagtggc ttaaaagcgg cgttgttgtt tattgatgct cagcaaaagc gcgttggcag
    32341 tgaaaccgcg tggatcaaga aaggggacga accgccgctc agcgtaccgc ccgcgcctgc
    32401 gctgaaagag gtcgcggtgg ttaacccaac gccgacgcca ctctcactcg aagaacgcaa
    32461 cgatttgctg gattatggaa actggcggat gaatggtctg cgctgctcgc ttgatccgtt
    32521 acgtcgtgag gtgaatgtca ctgcgctgac tgatgataaa gcgctgatga tgatcagctg
    32581 tgaggcaggg gcctataaca ccattgattt ggcatggatt gtgtcgcgta aaaagccgct
    32641 agcctcgcgc ccggttcggt tgcgtttgcc gttcaacaac gggcaggaga cgaatgaact
    32701 ggaactgatg aacgcaacat ttgatgagaa atcgcgtgaa ctggtgacct tagcgaaagg
    32761 gcgcggatta agcgattgtg gcattcaggc gcgctggcgc tttgacggtc agcgttttcg
    32821 cctggtgcgt tacgccgcag aacccacctg cgataactgg catgggccag acgcctggcc
    32881 cacgttgtgg atcacccgtt aaacaaaatg gcggggacat gtaggccgga taagacgctg
    32941 taggccggat aagacgcgtc agcgtcgcat ccggcaatca gcatccggcg atcactatca
    33001 ggcacctttc actcccagca ccttatgcgc ttttgccaca atattctcgg cggtaaagcc
    33061 aaagaacggg aacagcttat ccgccggagc agattcccca taacccgtca tcccgacaat
    33121 tgcccctttc agaccaacat acttgtacca gtaatcggca atacctgctt ccaccgccac
    33181 gcgagccgca acgttagaag gcaacaccga ctcccgatat tcctcatcct gggcgtcgaa
    33241 aatatcggtc gagggcaggg aaaccacgcg aacattgcga ccttctcctg ctaatttctc
    33301 cgcagcttgc agggtgattt ccatctctga accggtggca atcagaataa tatctggctt
    33361 accgccgctg tctttcagca cataaccgcc acgagtaatc tctttaacct gatccggcgt
    33421 acgttccacc tgggccagat tctgccttga gaggatcagt gccgtcggtc cgttgtggcg
    33481 ctcaaccgcc agcttccagc ccaccgccgc ttccacctga tcgcacggtc gccaggtgct
    33541 gaaatttggc gttaagcgca ggctggccag ttgctcaaca gcctggtgcg tcggaccatc
    33601 ttcgcccagg ccaattgagt cgtgggtata aaccataatc tgccgcgctt tcatcagtgc
    33661 cgccatccgc gcggcgttac gggcgtattc aacaaacatc aggaacgtcg cggtatacgg
    33721 cacaaagccg ccgtggtgcg cgatgccgtt ggcgatagcg gtcatgccaa attcacgcac
    33781 cccgtagtga atgtagttgc ccgccggatc ttccttcagc gaaacagaac ctttccagat
    33841 ggtcaggttg ctgggagcca gatccgccga accgccgagc agctccggta gcaaggggcc
    33901 ataagcgtta agcgtatttt gcgaagcctt acgggtggcg attttcgccg gattcgcctg
    33961 caactcattg atatacttct gagtcgtttt ctcccagtcc ttcggtaaac caccgctcat
    34021 ccgacgggta aactcttctg ccagttgcgg atgagccttt ttataggcgg caaacttctc
    34081 attccacctc tgctgcgctt tttcgccttt ttcgcgggca tcccaggcgt gataaatatc
    34141 tttagggatc tcaaatggcg gatggtgcca gcccagtttt tgccgtgcca gcgccacttc
    34201 ttcttccccc agcggtgcgc cgtgcgcctc ttccttacct gctttattcg gcgatccaaa
    34261 gccaatcacc gtacgacaga taatcagcga cggcttatct ttcacgcttt gcgcttcaag
    34321 gatcgcttcc ttcaccgcct gcggatcgtg accgtcgatt tcatggatca catgccagtg
    34381 ataggcttca aaacgttttg ccgtatcgtc ggtaaaccag ccttctgttt caccgtcgat
    34441 ggaaataccg ttgtgatcgt aaaaaccaat cagcttgccc agtcccagcg tgcctgccag
    34501 cgaacagact tcgtgggaaa taccttccat caggcagccg tcgcccataa acacataggt
    34561 gaagtgatcg acgatctcat ggtctggctg gttaaactgc gccgccagtg tacgctctgc
    34621 tatcgccagc ccgacggcgt tcgccaaacc ttgtccaagt ggaccggtgg tggtttcaac
    34681 gcctggcgta taaccaatct ccgggtggcc tggggttttc gaatgcagtt gacgaaagtt
    34741 cttcagctct tccagcggca ggtcgtaacc ggtcagatgc agcaaactgt agagcagcat
    34801 cgacgcgtga ccgttggaaa gaataaagcg gtcgcgatca taccaggttg ggtcggtagg
    34861 gttatgttta agaaaatcgt tccacagcac ttcggcaata tcagccatgc ccatcggcgc
    34921 gccgggatga ccagaattgg ctttttgtac cgcatccata ctgagtgcgc gaatcgcatt
    34981 ggcaaggtct tttcgggaca tataacactc cgtggctggt ttatagtttg gcggcaagaa
    35041 gatcttccag tttgcgttga tcaacggcga acagacgaat gccttcagac agtttttcta
    35101 ccgccatcgc atactgattg tgctcccaac ggaactccgc ttcgctcatg ggagctgggc
    35161 gtgggaacgt ctgagaaggt gggattaatt tacgtaccac tggcgaaact ttttcctgca
    35221 gctccttcag caaattcggt gcgatagtca gtcgatcgca gccggttaag gcgaggattt
    35281 gttcggtgcg acggaagctc gcgcccatca caatggtttc atagtggtgt tgcttatagt
    35341 agtcgtagat attgcgcacc gatttaacgc ccggatcttc ttccaccaca tacgggtcca
    35401 tcggcttgcg tgcctgatac cagtcataaa tacgcccgac aaacggcgaa atcagaaaaa
    35461 cgcctgcttc cgcacaggcc cgtgcctgtg caaaagaaaa cagcagcgtc aggttgcagt
    35521 taataccttc tttttccagc tcttctgccg cgcgaattcc ttcccaggtc gaagccagct
    35581 tgatcagaat gcgtgatttc tcaacgcctt gttgctgata caagtccacc agatggcgcg
    35641 ccttctcaat actcttttct ttatcaaaag agaggcgtgc atcaacttct gttgacacgc
    35701 gaccgggtac gattttgagg atttcagcac cgaaattgac cgccagtttg tcacacgctg
    35761 cgaccacctg ttgttcctgg gtcttgccat tttttttacc ccaggcgata gcatcgtcta
    35821 ttaaatgctc atattgtgat aatccggcag ccttgagtaa cagcgaagga ttggtggtgg
    35881 catcctgggg atgataatgg cgaatggact caatatcgcc gctgtctgcc acgacagtgg
    35941 tgaactgttt gatgccgtct aactcgttca taggaaatac tccttgaaaa gtaaagtgtt
    36001 agatgagtgc gttaattcac acttctgaga aatttcgcta aacgcatcaa aaaagcatag
    36061 cagacaggca tggtattgct ggattaagca ggtaacatca gtgttatagg attattacca
    36121 aaacattata ttaatggtga gagtttggac ttgctcaaag tctgtagact ccggcaaggt
    36181 aataatgtgc gccacgttgt gggcaggggg tgttgcccac acactttatt tgtgaacgtt
    36241 acgtgaaagg aacaaccaaa tggatgacca gttaaaacaa agtgcacttg atttccatga
    36301 atttccagtt ccagggaaaa tccaggtttc tccaaccaag cctctggcaa cgcagcgcga
    36361 tctggcgctg gcctactcac ccggcgttgc cgcaccttgt cttgaaatcg aaaaagaccc
    36421 gttaaaagcc tacaaatata ccgcccgcgg taacctggtg gcggtgatct ctaacggtac
    36481 ggcggtgctg gggttaggca acattggcgc gctggctggc aaaccggtga tggaaggcaa
    36541 gggcgttctg tttaagaaat tcgccgggat tgatgtattt gacattgaag ttgatgaact
    36601 cgacccggac aaatttattg aagttgtcgc tgcgctcgaa ccgaccttcg gcggaatcaa
    36661 cctcgaagac attaaagcgc cagaatgttt ctatattgaa cagaaactgc gcgagcggat
    36721 gaatattccg gtattccacg acgatcagca cggcacggca atcatcagca ctgccgccat
    36781 cctcaacggc ttgcgcgtgg tggagaaaaa catctccgac gtgcggatgg tggtttccgg
    36841 cgcgggtgcc gcagcaatcg cctgtatgaa tctgctggta gcgctgggtc tgcaaaaaca
    36901 taacatcgtg gtttgcgatt caaaaggcgt tatctatcaa ggccgtgagc caaacatggc
    36961 ggaaaccaaa gccgcatatg cggtggtgga tgacggcaaa cgtaccctcg atgatgtgat
    37021 tgaaggtgcg gatattttcc tgggctgttc cggcccgaaa gtgctgaccc aggaaatggt
    37081 gaagaaaatg gctcgggcac caatgattct ggcgctggcg aatccggaac cggaaattct
    37141 gccgccgctg gcgaaagaag tgcgtccgga tgccatcatc tgcaccggtc gttctgacta
    37201 tccaaaccag gtcaacaacg tcctttgctt cccgttcatc ttccgtggcg cgctggacgt
    37261 tggcgcaacc gccatcaacg aagagatgaa actggcggcg gtacgcgcga ttgcagaact
    37321 ggcccatgcg gaacagagcg aagtggtggc ttcagcgtat ggcgatcagg atctgagctt
    37381 tggtccggaa tacatcattc caaaaccgtt tgatccgcgc ttgatcgtta agatcgctcc
    37441 tgcggtcgcg aaagcggcga tggagtcggg cgtggcgacg cgtccgattg ctgatttcga
    37501 cgtctacatc gacaagctga ctgagttcgt ttacaaaacc aacctgttta tgaagccgat
    37561 tttctcccag gctcgcaaag cgccgaagcg cgtagttctg ccggaagggg aagaggcgcg
    37621 cgttctgcat gccactcagg aactggtaac gctgggactg gcgaaaccga tccttatcgg
    37681 tcgtccgaac gtgatcgaaa tgcgcattca gaaactgggc ttgcagatca aagcgggcgt
    37741 tgattttgag atcgtcaata acgaatccga tccgcgcttt aaagagtact ggaccgaata
    37801 cttccagatc atgaagcgtc gcggcgtcac tcaggaacag gcgcagcggg cgctgatcag
    37861 taacccgaca gtgatcggcg cgatcatggt tcagcgtggc gaagccgatg caatgatttg
    37921 cggtacggtg ggtgattatc atgaacattt tagcgtggtg aaaaatgtct ttggttatcg
    37981 cgatggcgtt cacaccgcag gtgcaatgaa cgcgctgctg ctgccgagtg gtaacacctt
    38041 tattgccgat acctacgtca atgacgaacc agacgcagaa gagctggcgg agatcacctt
    38101 gatggcggca gaaactgtcc gtcgtttcgg tattgaaccg cgcgttgctc tgttgtcgca
    38161 ctccaacttt ggttcttctg actgcccgtc gtcgagcaaa atgcgtcagg cgctggaact
    38221 ggtcagggaa cgtgcgccag aactgatgat tgatggtgaa atgcacggcg atgcggcgct
    38281 ggtggaagcg attcgcaacg accgtatgcc ggacagccct ttgaaaggtt ctgccaatat
    38341 tctggtgatg ccgaacatgg aagctgcccg cattagttac aacttactgc gcgtttccag
    38401 ctcggaaggt gtgactgtcg gtccggtgct gatgggcgtg gcgaaaccgg ttcacgtgtt
    38461 aacgccgatc gcatcggtgc gtcgtatcgt caacatggtg gcgctggccg tggttgaagc
    38521 gcaaacccaa ccgctgtaat tcttttttaa ctctcacgct taccctgaat attcagggta
    38581 agcggtttgg ctgcaatata ttagtaaagc ttattactga gtttgcgaat aataaaaaaa
    38641 gcagtctata taatatctcg atattatttt atttatattc atgcgttgca tatgaaagtt
    38701 tatgcaccac agcgaatatc tctccattct tagtgatcta cctcaccttt taaacgcgct
    38761 tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa gtttttcaca
    38821 ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca
    38881 cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc ggcgttccgg
    38941 atgcgggcgt aattggcatt atgacgctaa ctcccggcga aacggcgatg atcgtcggtg
    39001 atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc agcggtgcat
    39061 tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt
    39121 taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac gaggcattca
    39181 tgaaacgaat tgcgtttgtg ggtagcgtcg gggcaggaaa aacaacgtta tttaatgcgt
    39241 tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat gataagggcg
    39301 atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc ttaattacca
    39361 cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg gaaagtcggt
    39421 tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc atcagtaaaa
    39481 cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg
    39541 aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa ctggtggatt
    39601 atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac agcgaatgat
    39661 attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg cgccagcatt
    39721 attaccccgg aagcccgtaa agtggcggat ctactgggtt ttaccatcac cgaatgtgat
    39781 gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc
    39841 cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac cgaaagcctg
    39901 gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctggagca gggcgcaatg
    39961 cagccgagct tcaaatcggt gaccggcaaa ggcggcatca aagtgattga tggcagcagc
    40021 gtcaagtttg gtcgttttga tggcgcacaa ccgcattgcg tgggcttgac tgatcttgtg
    40081 accggagacg acggcagcag catggccgcc ggttttatgc agtgggagaa cgcattcttc
    40141 ccgtggaccc tgaactacga cgaaatcgac atggtgctgg aaggggagct ccatgttcgc
    40201 cacgaaggcg aaaccatgat cgccaaagca ggcgacgtga tgtttatccc gaaaggctcc
    40261 agcatcgaat ttggtacgac atccagcgtg aaattcctgt atgtcgcctg gccggctaac
    40321 tggcaatccc tatgaaagat ttcatcaccg aagcatggct aagagcgaac catacgctca
    40381 gcgaaggcgc agagatccat ctccctgcgg acagtcgcct gacgccctct gcccgggagt
    40441 tgctggaaag ccgccatctg cgcatcaagt ttattgacga gcagggccgc ctgtttgttg
    40501 acgatgaaca gcagcagccg cagcccgttc atgggctgac cagtagcgat gaacatccgc
    40561 aggcgtgctg cgaactgtgc cgccagccgg tggcaaaaaa gccggatacg ctgactcacc
    40621 tgtcggcgga aaaaatggtc gccaaaagcg atccgcgtct gggttttcgc gcggttctcg
    40681 acagcaccat tgcgctggca gtgtggctgc aaattgaact ggcggaaccg tggcagccgt
    40741 ggctggcgga tatccgttcg cgtcttggca acattatgcg cgccgatgcg ctgggggaac
    40801 cgctgggctg ccaggcgatt gtcgggcttt ctgacgaaga tctgcatcga ctttctcacc
    40861 agccgctgcg ctatctcgac cacgatcatc tggtgccgga agccagccac gggcgcgatg
    40921 cggcgttgct caatctgcta cgtaccaaag tgcgggaaac cgaaaccgta gcggcgcagg
    40981 tgtttatcac ccgcagcttt gaagtattac gcccggacat cttgcaggcg ctgaaccgcc
    41041 tttcgagcac ggtctacgta atgatgatcc tgagcgtaac gaagccgccg ctaacggtga
    41101 agcagatcca acaacgactg ggggaaacgc aatgattatt gaacgttgtc gtgaactggc
    41161 gttgcgagcg cccgccagag tggtttttcc ggatgcgtta gatcaacgtg tgctgaaagc
    41221 tgcgcaatat ttacatcaac aaggtctggc aacgcccatt ctggtcgcta atccgtttga
    41281 acttcgtcag tttgcgctca gtcacggcgt ggcgatggac gggctacagg tgattgatcc
    41341 gcatggcaac ctcgcaatgc gggaagaatt tgctcatcgc tggctggccc gcgcgggcga
    41401 aaaaacgccg ccggatgcgc tggaaaaact taccgatccg ctgatgttcg ccgcagcaat
    41461 ggtcagcgcc ggtaaagcgg atgtctgtat cgcgggcaac ctctcttcca cggcgaatgt
    41521 gctgcgtgcc ggattacgta ttatcggctt gcagccaggc tgtaaaacgc tctcatccat
    41581 tttcctgatg ctgccacagt acagcggtcc ggcgttgggc tttgccgatt gcagcgtggt
    41641 gccacagccg acggcggcgc aactggcgga tatcgcgctt gccagtgccg aaacctggcg
    41701 cgccctcacc ggagaagagc cgcgcgtggc gatgctgtcg ttttccagta acggtagcgc
    41761 ccgtcacccc tgcgttgcca acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc
    41821 aaagctggtg gttgatggcg agttgcagtt tgacgccgcc ttcgtgccgg aagtggcggc
    41881 gcaaaaagcg cctgccagcc cgctacaggg caaagccaat gtgatggttt ttccgtcgct
    41941 ggaagccgga aatattggct acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg
    42001 gccattgata caaggacttg ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca
    42061 ggaaattatc gagctggcgc tggtggcagc tgtgccgcgt cagacagaag tgaaccgcga
    42121 aagcagttta caaacactgg ttgaatgaac ggtcccgttc tggaccctga taaagaggaa
    42181 agcacaatgg aagcattagg aatgatcgaa acccggggcc tggttgcact gattgaggct
    42241 tctgacgcga tggttaaagc tgcgcgcgtc aagctggttg gtgttaagca gattggcggc
    42301 ggcctgtgta ctgccatggt tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca
    42361 ggtgccgcag cggcacagcg tatcggcgag ctggtatctg tacacgttat cccgcgtccg
    42421 cacggcgacc tggaagaagt cttcccgatc ggcctgaaag gcgatagcag caacctgtaa
    42481 tcaccctgtg tgacgtcgat tcgggcgtca cgctctctcc tttttcattt taccttctgc
    42541 ggagggtagg gggagctttt acctgaaata cgccacggag gcgggtatga aactggcagt
    42601 cgtcactgga caaattgttt gtaccgtacg ccatcacgga ctggcgcatg acaaattgct
    42661 gatggtggaa atgattgatc cacaaggtaa tcctgacggg caatgcgccg tcgccatcga
    42721 caatattggc gcgggaaccg gggagtgggt gttactggtg agtggcagtt ccgcccgcca
    42781 ggcgcataaa agcgaaacgt caccggtcga tctgtgcgtg attggcattg tcgatgaggt
    42841 ggtgtctggc ggtcaggtaa ttttccacaa ataaggcaga acatcatgaa tcaacaggat
    42901 attgaacagg tggtgaaagc ggtactgctg aaaatgcaaa gcagtgacac gccgcccgcc
    42961 gccgttcatg agatgggcgt tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc
    43021 gcccagcaag ggttaaaaag cgtggcaatg cgccagttag ccattgctgc cattcgtgag
    43081 gcaggcgaaa aacacgccag agatttagcg gaacttgccg tcagtgaaac cggcatgggc
    43141 cgcgttgaag ataaatttgc caaaaacgtc gctcaggcgc gcggcacgcc aggcgttgag
    43201 tgtctctctc cgcaggtgct gaccggcgac aacggcctga ctttaattga aaacgcgccc
    43261 tggggcgtgg tggcttcggt aactccgtcc actaacccgg cggcaaccgt aattaataac
    43321 gccatcagcc tgattgccgc gggcaacagc gtcatttttg ccccgcatcc ggcggcgaaa
    43381 aaagtctccc agcgggcgat cacgctgctc aaccaggcga ttgttgccgc aggcgggccg
    43441 gaaaacttac tggttactgt ggcaaatccg gatatcgaaa ccgcgcagcg tttgttcaag
    43501 tttccgggca tcggccttct ggtggtgacc ggcggcgaag cggtagtaga agcggcgcgt
    43561 aaacacacca ataaacgtct gattgccgca ggcgctggca acccgccggt agtggtggat
    43621 gaaaccgccg acctcgcccg tgccgctcag tccatcgtca aaggcgcttc tttcgataac
    43681 aacatcattt gtgccgacga aaaggtactg attgttgttg atagcgtagc cgatgaactg
    43741 atgcgtctga tggaaggcca gcacgcggtg aaactgaccg cagaacaggc gcagcagctg
    43801 caaccggtgt tgctgaaaaa tatcgacgag cgcggaaaag gcaccgtcag ccgtgactgg
    43861 gttggtcgcg atgcggcgaa aatcgcggcg gcaatcggcc tgaacgtgcc gcaagaaacg
    43921 cgtttgctgt ttgtggaaac cactgcagaa catccgtttg ccgtgaccga actgatgatg
    43981 ccggtgctgc ccgtcgtgcg cgtcgccaac gtggcggatg ccattgcgct ggcggtgaaa
    44041 ctggaaggcg gttgccacca cacggcggca atgcactcgc gcaacatcga aaacatgaac
    44101 cagatggcga atgctattga taccagcatt ttcgttaaga acggaccgcg cattgccggg
    44161 ctggggctgg gcggggaagg ctggaccacc atgaccatca ccacgccaac cggtgaaggg
    44221 gtaactagcg tgcgtacgtt tgtccgtctg cgtcgctgtg tattagtcga tgcgtttcgc
    44281 attgtttaag gagtaacaga tggcgcacga cgaacaatgg ctcaccccac gtctgcaaac
    44341 ggcggcaacg ctgtgtaacc agacgcccgc cgcgacggaa tctccgctgt ggctgggggt
    44401 ggatctgggc acctgcgatg tggtgtcgat ggttgtcgac cgcgacggtc agtcggtggc
    44461 ggtatgcctc gactgggccg atgtggtccg cgacggcatc gtctgggatt tcttcggcgc
    44521 tgtcaccatt gttcgtcgcc atctcgacac gctcgagcag caattcggtc gccgttttag
    44581 ccatgcggcg acttcatttc ctccaggtac ggacccgcgt atttccatca acgtgctgga
    44641 gtctgctgga ctggaagtga gccatgtgct ggatgagcca accgcagttg cggatctgct
    44701 gcaactggac aacgccggtg tagtggatat cggcggcggc actaccggca ttgccatcgt
    44761 gaaaaagggc aaggtgacgt actcggcgga tgaagcaacc ggcggacatc acatctctct
    44821 gactctcgcc ggaaatcgcc gtatttcgct ggaagaggcg gagcagtaca agcgcggtca
    44881 cggtgaagag atttggccta cggtgaaacc ggtgtacgaa aaaatggtgg acattgtcgc
    44941 ccggcatatt gaagggcagg gcattactga tttatggctg gcgggcggct cttgtatgca
    45001 accgggcgtg gcggaactgt ttcgcaaaca attcccggcg ttacaggtgc atttaccgca
    45061 gcacagtttg tttatgaccc cgctggcgat cgccagtagt gggcgagaga aagcggaggg
    45121 gctatatgca aaatgaattg cagaccgcgc tctttcaggc gttcgatacc ctgaatctgc
    45181 aacgggtaaa aacatttagc gttccaccgg tgacgctttg cggtccgggc tcggtgagca
    45241 gttgcggaca gcaagcgcaa acgcgtgggc tgaaacatct gttcgtgatg gcagacagct
    45301 ttttgcatca ggcagggatg accgccgggc tgacgcgcag cctggctgtt aaaggcatcg
    45361 ccatgacgct ctggccatgt ccggtgggcg aaccgtgcat caccgacgtg tgtgcagccg
    45421 tggcgcagtt gcgtgagtca ggctgtgatg gggtgatcgc atttggcggc ggctcggtgc
    45481 tggatgcggc gaaagccgtg gcgttgctgg tgacgaaccc ggatagcacg ctggcagaga
    45541 tgtcagaaac cagcgttctg caaccgcgct tgccgctgat tgccattcca acgaccgccg
    45601 gaaccggctc tgaaaccacc aatgtaacgg tgattatcga cgcggtgagc gggcgcaagc
    45661 aggtgttagc ccatgcctcg ctgatgccgg atgtggcgat tctcgacgcc gcgttgaccg
    45721 aaggtgtgcc gtcgcatgtc acggcgatga ccggcattga tgcgttaacc catgccattg
    45781 aagcatacag cgccctgaac gctacaccgt ttaccgacag tctggcgatt ggtgccattg
    45841 cgatgattgg caaatcgctg ccgaaagcgg tgggctacgg tcacgacctt gccgcgcgcg
    45901 agagcatgtt gctggcttca tgtatggcgg gaatggcgtt ttccagtgcg ggtcttgggt
    45961 tgtgccacgc gatggcgcat cagccgggcg cggcgctgca tattccacac ggtctggcga
    46021 acgccatgtt gctgccaacg gtgatggaat ttaaccggat ggtttgtcgt gaacgcttta
    46081 gtcagattgg tcgggcactg cgaactaaaa aatccgacga tcgtgacgct attaacgcgg
    46141 taagtgagct gattgcggaa gttgggattg gtaaacgact gggcgatgtt ggtgcgacat
    46201 ctgcgcatta cggcgcatgg gcgcaggccg cgctggaaga tatttgtctg cgcagtaacc
    46261 cgcgtaccgc cagcctggaa cagattgtcg gactgtacgc ggcggcgcaa taaatgccgg
    46321 atgcgacgct tgccgcgtct tatccagcct acgggattgc acatgtaggg cggataaggc
    46381 gttcacgccg catccgccag tggcgcggtg caattgccgg atgcgatgct tgccgcgtct
    46441 tatccggcct acgggattgc acatgtaggg cggataaggc gttcacgccg catccgccag
    46501 tggcgcggtg caattgccgg atgcgacgct tgccgcgtct gatccggcct tcgggattgc
    46561 acatgtaggg cggataaggc gtttacgccg catccgccaa taaataacaa ctaacaggga
    46621 gtaaaggcga tgggaattaa cgaaatcatc atgtacatca tgatgttctt tatgctgata
    46681 gctgccgtag acaggatcct gtcgcagttc ggcggttctg ctcgtttcct cggtaagttc
    46741 ggtaaaagta tcgaaggatc aggcggtcag ttcgaagaag gctttatggc aatgggcgct
    46801 ctgggtctgg cgatggtcgg tatgaccgcg ctggcaccgg tactggctca cgtactcggg
    46861 ccggtaatta tcccggttta cgaaatgctc ggcgcaaacc cgtcaatgtt cgccggaacg
    46921 ctgctggcgt gcgatatggg cggcttcttc ctcgccaaag agctggcggg cggcgacgta
    46981 gcagcgtggc tatactctgg gttaattctt gggtcgatga tggggccaac gattgtgttt
    47041 tccattccgg tggcgctcgg cattatcgaa ccttctgacc gtcgttatct ggcgctcggc
    47101 gtgctggcgt gcattgtgac cattccgatt ggctgtattg ctggtggtct ggttgctatg
    47161 tactccggtg tgcagatcaa cggtcagccg gtggaattca ctttcgccct gatcctgatg
    47221 aacatgatcc cggtgatcat tgttgcgatt ctggtggcgc tggggctgaa attcatcccg
    47281 gaaaaaatga tcaacggctt ccagatcttc gccaaattcc tcgttgcatt gatcaccctc
    47341 ggtcttgctg ccgcggtagt taagtttctg cttggctggg aactgatccc cggtctggac
    47401 cctatcttta tggcccctgg cgataaaccc ggtgaagtga tgcgcgccat tgaagttatc
    47461 ggttctatct cctgcgttct gttaggggcg tatccaatgg tgctgctgct gacccgctgg
    47521 tttgaaaaac cgctgatgag cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc
    47581 ggcatggtgg caacgcttgc caacaacatc ccgatgttcg gcatgatgaa gcagatggat
    47641 acccgcggca aagtcatcaa ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac
    47701 cacttaggct tcgccgctgc caacatgaac gccatgatct tcccgatgat tgtcggcaag
    47761 ttgatcggcg gcgtaacggc gattggcgtg gcgatgatgc tggtgccaaa agaagacgcg
    47821 accacggcta aaaccgaagc ggaggcacaa tcgtgaacac tcgccagcta ttgagcgtcg
    47881 gtatcgatat cggcaccacc accacccagg tgattttctc ccacctggag ctggttaacc
    47941 gtgcggcggt gtcgcaggtg ccgcgctacg aattcattaa acgcgaaatt agctggcaaa
    48001 gcccggtgtt ctttacccct gtcgataaac agggtggttt aaaagaagcg gaactgaaaa
    48061 ccttaatcct cgagcaatat caggctgcgg gcattgcgcc ggaaagcgtt gattctggtg
    48121 ccatcatcat caccggtgaa agcgcgaaaa cccgcaatgc tcgtccggcg gtgatggtgc
    48181 tctcccagtc gctgggcgat tttgtcgttg ccagcgccgg gccgcacctc gaatccgtca
    48241 ttgccggtca cggtgccggg gcacaaaccc tttctgaaca acggctgtgt cgggtactga
    48301 atattgacat cggcggtggc actgcgaact acgccctgtt cgatgccgga aaaatcagcg
    48361 gcactgcctg cctcaacgtc ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg
    48421 tttacgctca taaaccgggg cagatgattg tggatgagtg cttcggcgca ggcaccgacg
    48481 cccgttcgct gaccggcgcg cagctggtgc aggttacccg gcggatggca gaactgattg
    48541 tcgaagtgat tgacggaacg ctctcgccgc tcgcgcaggc attgatgcaa accggtttgc
    48601 tgcccgcagg tgttacgccc gaaatcatta cgctttctgg cggcgtgggc gaatgttatc
    48661 gccaccagcc cgccgacccg ttctgttttg ccgatattgg cccgctgctg gcaacggcgc
    48721 tgcatgacca tccgcgcctg cgtgagatga acgtacagtt tccggcgcaa accgtgcgcg
    48781 ccacggtgat tggcgcgggc gcgcataccc tttcgctctc tggcagcaca atctggcttg
    48841 agggcgtaca actgccgctg cgcaatttgc cggtggcgat cccgattgat gaaacggatc
    48901 tggtgagtgc ctggcaacag gcgctgcttc agctggatct tgatcccaaa actgacgcgt
    48961 acgtgctggc gcttcccgcc tcgctgcctg tgcgttacgc cgcggtactg acggtcatca
    49021 acgcgctggt cgatttcgtc gcgcgttttc caaatccgca tcccctgctg gtggtggccg
    49081 ggcaggactt tggtaaagct ctgggcatgt tgttgcgccc acagctacaa caactcccgt
    49141 tggcagtcat tgacgaagtg attgtccgcg cgggggacta tatcgacatt ggtacgcctc
    49201 tttttggcgg atcggttgtg ccggtgacgg tgaaatcact cgcatttcct tcctgaggga
    49261 acgacttatg aaactaaaga ccacattgtt cggcaatgta tatcagttta aggatgtaaa
    49321 agaggtgctg gctaaagcca acgaactgcg ttcgggggat gtgctggcgg gcgttgcagc
    49381 ggcaagctca caggagcgcg tggcggcaaa gcaggtgttg tcggaaatga ccgtagcgga
    49441 catccgcaat aatccggtga ttgcctatga agatgactgc gtgacgcggc tgattcagga
    49501 cgatgttaac gaaacggcct acaaccagat taaaaacagg agcatcagcg aactgcgtga
    49561 gtatgtgctg agcgatgaaa ccagcgtgga cgacattgcc tttacccgca aagggctgac
    49621 ctcggaagtg gtcgcggcgg tagcgaagat ttgctccaac gcggacctga tctacggcgc
    49681 gaagaaaatg ccggtaatca aaaaggccaa taccaccatc ggtattccgg gcaccttcag
    49741 cgcccgtttg cagccaaatg acacccgtga cgacgtgcaa agtatcgctg cacaaatcta
    49801 cgaagggctt tccttcgggg tgggcgatgc ggtgatcggc gttaacccgg taactgacga
    49861 cgtggaaaac ttaagccgcg tgttggacac catttacggc gtgatcgaca aattcaacat
    49921 cccaactcag ggctgcgtac tggcgcacgt caccacccag atcgaagcga ttcgtcgcgg
    49981 cgcgcctggc ggactgattt tccagagtat ctgtggcagc gaaaaagggc tgaaagagtt
    50041 tggcgtggaa ctggcgatgc tcgacgaagc gcgcgcagtg ggtgctgagt tcaaccgtat
    50101 cgccggggaa aactgcctct acttcgaaac cgggcaaggt tcggcgctgt ctgctggcgc
    50161 taacttcggc gcagactagg tgacgatgga agcacgtaac tatgggctgg cgcgtcatta
    50221 cgatccgttt atcgtcaaca ccgtggtcgg ctttattggg ccggagtatc tctacaacga
    50281 ccgccagatt atccgtgctg gcttagaaga tcactttatg ggcaagctga gcggcatctc
    50341 tatgggctgt gactgctgtt acaccaacca cgctgatgct gaccagaacc tcaacgaaaa
    50401 cctgatgatc ctgctcgcca ccgcaggctg caactacatc atggggatgc cgctgggcga
    50461 tgacatcatg ctcaactatc agaccaccgc attccacgac actgccactg tgcgtcagtt
    50521 actcaacctg cgcccgtcac cggagtttga acgctggctg gaaagcatgg gcattatggc
    50581 aaacggtcgc ctgaccaaac gggcgggcga tccgtcactg ttcttctgat gacgcggaga
    50641 taacacatca tggatcaaaa acagattgaa gaaattgtac gcagcgtgat ggcgtcaatg
    50701 ggacaagcgg cccccgcgcc gtcagaagca aagtgcgcca ccactaactg tgcggcaccg
    50761 gtgacctcgg aaagctgcgc gctggattta ggttctgcgg aagcaaaagc gtggattggt
    50821 gttgaaaacc cgcatcgcgc agacgtatta acagaactgc gccgcagcac cgtggcccgc
    50881 gtttgtaccg gtcgtaccgg tccgcgtcca cgtacgcagg cgctgctgcg tttcctggcc
    50941 gatcactctc gttcgaaaga taccgtactg aaagaagtgc cggaagagtg ggtgaaagcg
    51001 cagggcttgc tggaagtacg ctccgaaatt agcgacaaaa atctctacct gactcgcccg
    51061 gatatgggcc gccgcctgtg tgcagaagct gttgaagcgc tgaaagcaca gtgtgttgct
    51121 aatccggacg tacaggtcgt tatttctgat ggcctgtcaa ctgatgccat caccgtgaac
    51181 tacgaagaga tcctgccgcc gctgatggcg ggcctgaaac aggccgggct gaaagtcggc
    51241 acgccattct ttgttcgtta tggtcgcgtg aagattgaag atcagatcgg tgagatcctc
    51301 ggcgcgaaag tggtgatcct gctggtgggg gaacgtccgg ggttgggtca gtcagaaagc
    51361 ctctcctgtt acgccgttta ctcgccgcgt atggcgacca ccgttgaggc cgatcgcacc
    51421 tgcatctcta acattcacca gggcggcacg ccgccggttg aagccgcagc cgtcattgtg
    51481 gatttggcga aacgtatgct ggagcagaaa gcatccggca tcaacatgac ccgataagga
    51541 ggcatcatgc cagctttaga tttgattcga ccgtcggtaa ccgctatgcg ggtgattgcc
    51601 tccgttaacg ctgaatttgc gcgtgaacta aaattgccgc cgcatattcg tagcctcggg
    51661 ctgatttctg ctgattccga tgacgtcacc tacattgcgg ctgacgaagc gaccaagcag
    51721 gcgatggtcg aagtggtata tggccgctcg ctgtatgcgg gagcagcaca cggaccgtca
    51781 ccgactgccg gtgaagtgct gattatgctt ggtgggccaa acccggcgga agtgcgtgct
    51841 ggtctggatg cgatggttgc gcatattgaa aatggcgctg ctttccagtg ggctaatgac
    51901 gcagaaaaca ccgcattcct ggcacatgta gtttcgcgta ccggttctta tctttcatca
    51961 accgccggga tcacgcttgg cgatccgatg gcgtatctgg tggcaccgcc gctggaagcg
    52021 acctacggca ttgatgcagc gttgaaatct gccgacgtac agctggtgac ctatgtcccg
    52081 ccaccgtctg aaaccaacta ctcggcagca tttttaaccg gtagccaggc cgcgtgtaaa
    52141 gcagcctgta acgcctttac cgatgcagtg ctggaaatcg cgcgtaatcc aatccagcgt
    52201 gcgtaacgga ggttgccgat gatcaatgca ctgggattgc tggaagtgga cggcatggtc
    52261 gccgcgatag atgcagcgga tgccatgctc aaagcagcta acgttcgtct gctcagtcac
    52321 gaagtgcttg accctggtcg cttaacgctg gtggtggaag gcgatctggc ggcgtgtcgt
    52381 gcggcgctgg acgcaggttg tgctgccgcg atgcgtaccg ggcgtgtcat cagccgcaag
    52441 gagatcggtc ggccagacga tgacacccag tggctggtta ctggctttaa ccgccagccg
    52501 aagcaacccg taaaggaacc cgacgcgcca gttatcgtcg cggaatctgc tgacgagttg
    52561 ttggcgctgt taacatcagt acgtcaggga atgacggcag gagaagtggc tgcccacttt
    52621 ggctggccgc tggaaaaagc cagaaatgcg ctcgaacagc tcttttctgc cgggacgtta
    52681 cgtaaacgca gtagtcgtta tcgtctcaag ccccattaac ctgtcggagg tgccgggtgt
    52741 cctacaacac ccggcattaa catcatgaaa aagacccgta cagccaattt gcaccatctt
    52801 tatcatgaac ccttacccga aaacctgaag ctcacgccga aggtcgaagt ggataatgtt
    52861 catcaacgac agacaacgga tgtctatgaa catgctttga cgattaccgc ctggcagcag
    52921 atttacgatc agctgcatcc gggcaagttt catggtgaat ttacggaaat tctgctcgat
    52981 gatattcagg tttttcgtga atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg
    53041 ccgaactcgt tctggtttgg cattccggcg acgcgcggtg agcagggatt tatcggttcg
    53101 caatgtctgg gaagcgcaga aatagcgacg cgccccggtg gaaccgagtt tgaattaagt
    53161 acgccggacg attatacgat cctgggcgta gtgctgtctg aagatgtcat cacccgtcag
    53221 gctaactttt tgcacaatcc ggatcgggta ttacatatgc tgcgtagcca gtcggcgctg
    53281 gaagtgaaag agcagcataa agccgcgctg tggggctttg tccaacaggc gctggcgacg
    53341 ttttgcgaga acccggaaaa tctccatcag cctgcagtgc gaaaagtgct gggggataat
    53401 ttgctaatgg cgatgggggc gatgctggaa gacgcgcaac caatggtgac ggcggaaagc
    53461 atcagtcatc agagttaccg tagattactt tcccgcgccc gtgaatatgt gctggaaaat
    53521 atgtctgagc tggtgacggt gctggacttg tgtaatcaac tgcatgtcag tcgccgcacg
    53581 ctacaaaacg cgtttcacgc tattttaggc attggcccaa acgcgtggct gaaacgcatt
    53641 cgcctgaacg ccgtacgccg cgaactgata agcccgtggt cgcaaagcac aacggtaaaa
    53701 gacgccgcca tgcagtgggg attctggcat ctggggcaat ttgccacgga ttaccagcag
    53761 ctgtttgccg agaagccgtc gttgacgttg catcagcgga tgcgggaatg ggggtgaggg
    53821 agttacaccc aatccctgac cttaataaac tcacttaacg ccgcttctgg gctgccatct
    53881 tttggctgat aatcatactc ccagcgtacc agtggcggca ttgacatcag gatcgactcg
    53941 gtgcgcccgc cagtttgcag gccaaacaga gtgccgcgat cccagaccag attgaactcg
    54001 acataacgac cgcgacggta gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct
    54061 ttacgtcgct ctacaattgg taaataagcg tcggtgtagc ctttgcctac cgcctgcata
    54121 aaggcaaaac agtggtcgaa atctggcgtg ttcagatcat caaagaacag cccgccaata
    54181 ccgcgctgtt cgttgcgatg tttgaggtag aagtaatcgt cgcaccactt tttgtaacgg
    54241 ggataaacgt cttcaccaaa tggcaggcac aggtcacggg cggtgcggtg ccagtgaatg
    54301 gcgtcttctt caaaaccata gaaaggggtt aaatcgaagc cgccgccaaa ccaccagacg
    54361 ggctctgcac ccggtttttc ggcaataaaa aaacgcacat tcgcgtggct ggtgggaaca
    54421 tacgggttat gcggatgcac taccagtgaa acgcccatcg cctcgaaact gcgcccggca
    54481 agttccgggc gatgagcggt ggcggaagca ggcatcgcct cgccgtggac gtgagaaaag
    54541 ttgacgcctg cctgttcgaa aacaccacca ttacgcaaca cccgactacg cccgccgccg
    54601 ccagcttcgc gctgccaact atcttcgaca aattctgcgc catcgacggc ggtcagctgc
    54661 tgacaaatcg tatcctgaag gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc
    54721 ataacttatc gctttttcga atgtgctttc tggttgtcga accagtggaa ataactgatc
    54781 acgccttcag caatcgctgt ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc
    54841 tcttcttccg ggttggtgat aaacgaggtt tccaccagca ccgaaggaac cgacggcgat
    54901 ttcaacacca caaacgcggc ttgttcggtg ttgcggctgt gcagtttatg caccggctta
    54961 atctttttca gaatatgcga gccaagcgtc aggctgtttt taatagtatc ggtttgcacc
    55021 agatcaaaca gcacttgctg caataggtga tccttgtcag tcgccttttt accggcaact
    55081 tcatcggcgc ggttttcgcg ttcagacagg tatttcgcca ttgcgctact tgccccacgg
    55141 ttagagaggg caaataccga agcaccggca gctttcgggt tggtaaagcc atcggcatga
    55201 attgacataa acagatctgc gccatgttta tgggcgattt caacgcgatc gtaaagtggg
    55261 ataaacgtat cgccagaacg cgttaaacgc gcatcaatcc catgattacg caaaatggaa
    55321 cggacgtttt tagcaatcgc cagcaccaca tgtttttctt tcgaaccgtt gcgtccgatc
    55381 gctccggtat caattccgcc gtggcctgga tcgagaacaa cgacacgttt gccgccagat
    55441 tttttggctt tcggcttgct gtgtccgttg ctggttttta aaggttcgtc tttggcgatg
    55501 gcttgcgaca ttcctgacaa cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc
    55561 gaagtgagtg tttttagtgg tttaaaagtg ctcatacggc ctgagttgta ataataaagt
    55621 tccagatgtt atatcgtatc gcgtaatccg ttacgacggt ttgatgttga gaattgtttt
    55681 acttttcatt tcaatagttg acaactcccc attatgccac tttttcaacc gattttcgtc
    55741 agatattggc taattcgacc gttcacgcca taatcagcct tttaacccgc aaaaaggtaa
    55801 cgaataccat ggagatacgc gtatttcgcc aggaagattt cgaagaggtc atcacccttt
    55861 gggagcgttg cgacttgctg cgtccgtgga acgatccgga aatggacatc gagcgtaaga
    55921 tgaaccatga cgtcagtttg tttttggtcg ctgaggtaaa cggtgaagtg gtcggaacgg
    55981 tgatgggcgg ttatgacggg catcgcgggt ctgcttatta tcttggcgtg catccagagt
    56041 ttcgtgggcg tgggattgcc aatgcgttgc ttaatcggct ggagaaaaag ctgattgctc
    56101 gtggctgccc gaaaattcag atcaatgtgc cggaagataa cgacatggtg ctcggaatgt
    56161 atgaacgcct gggatatgaa cacgccgacg tgctgagtct gggtaagcgt ttgattgaag
    56221 atgaagagta ctgagttcca tcctgtccat tatgatgcgc acggacgcct gcgtttaccc
    56281 ttgctcttct ggcttgtgct attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc
    56341 ggtgcgtcgc gcgagcaggg tactgcgctg ctaaatctgt tttatcccga tcacgataat
    56401 ttctggctgg ggctgattcc aggcattccg gcggtgctgg cgtttttgtt gagcggtcgg
    56461 cgggctacat ttcctcgcac ttggcatgtg ctctattttc tgttgctgtt ggcgcaggtg
    56521 gttttacttt gctggcaacc gtggctgtgg ctgaacggtg aatccgttag cggtatcggt
    56581 ctggcgctgg tagtggcgga tattgtggcg ttaatctggc tgctgaccaa tcgacgttta
    56641 cgcgcttgtt ttaatgaaga aaaagaataa cggcactttt tggtgaattt gcactccaag
    56701 caacgttatt gaataaccaa aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct
    56761 atgccgttga tgctgaccgg ctgttccacg atgtcgtcag ttaactggtc ggccgctaac
    56821 ccgtggaact ggtttggatc gtccaccaaa gtgagcgagc agggcgtggg tgaattaacg
    56881 gcgtccacac cactgcaaga acaagccatt gctgatgcgc ttgatggcga ttatcgcctg
    56941 cgcagcggaa tgaaaaccgc gaacggcaac gtggtgcgct tttttgaagt gatgaaaggc
    57001 gacaacgtgg cgatggtgat taacggcgat caaggcacga tcagtcgcat tgacgtgctg
    57061 gatagcgata ttcctgctga caccggtgtt aaaatcggta caccgtttag cgacctttac
    57121 agcaaagcat ttggcaattg ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt
    57181 aaagccgaag gcagtcaaca tattagctac cagttcagcg gggaatggcg tggtcctgaa
    57241 gggttaatgc cttcggacga taccctgaaa aactggaaag tcagtaaaat tatctggcgg
    57301 cgttaatttg cgtctgaaca aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca
    57361 atgccacgga ttgcgtggca ttcttatttt caggaggaac aatgtctcag gttcagagtg
    57421 gcattttgcc agaacattgc cgcgcggcga tttggatcga agccaacgtg aaaggggaag
    57481 ttgacgccct gcgtgcggcc agtaaaacat ttgccgacaa actggcaact tttgaagcga
    57541 aattcccgga cgcgcatctt ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc
    57601 tgagcggcgg cgttggggca gaagagctga aagattttcc gggctacggt aaaggccttg
    57661 cgccgaccac ccagttcgat gtgttgatcc acattctttc tctgcgtcac gacgtaaact
    57721 tctctgtcgc ccaggcggcg atggaagcct ttggtgactg cattgaagtg aaagaagaga
    57781 tccacggctt ccgttgggtg gaagagcgtg acctgagcgg ctttgttgac ggtacagaaa
    57841 acccggcggg tgaagagacg cgtcgcgaag tggcggttat caaagacggc gtggatgccg
    57901 gcggcagcta tgtgtttgtg cagcgttggg agcacaacct gaagcagctc aaccggatga
    57961 gcgttcacga tcaggagatg atgatcgggc gcaccaaaga ggccaacgaa gagattgacg
    58021 gcgacgaacg tccggaaacc tctcacctca cccgcgttga tctgaaagaa gatggcaaag
    58081 ggctgaagat tgttcgccag agcctgccgt acggcaccgc cagtggcact cacggtctgt
    58141 acttctgcgc ctactgcgcg cgtctgcata acattgagca gcaactgctg agcatgtttg
    58201 gcgataccga tggtaagcgt gatgcgatgt tgcgtttcac caaaccggta accggcggct
    58261 attacttcgc gccgtcgctg gacaagttaa tggcgttgta agcgtttctg attcgatgat
    58321 taaaggccag ccagtaacaa aactgactgg cctttttatt acttttgctt caacgccgca
    58381 tacacctgat caactaccca accataagtt gcaatcggta actgaccgct ttcgaacata
    58441 ttgggatttt tttgtggatc ggcaactggc gaatggggct tgttgcttaa catcacaatc
    58501 gccatatgat tgaccggatc gataacggtt actgtcccgg tccagccagt gtgaccgtag
    58561 gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc
    58621 cagccgaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt caccgtttcc
    58681 gcactgaaca gctgcacatc gccatagccc ccgccgttca gcatcgtttg cattaacacc
    58741 gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa
    58801 aaggcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt cgggaaatgg
    58861 atcacgccat cgcgggtatt gccgttcaat tccgtggcgg caatttgttg cggtttaaag
    58921 cctttcagca gcgggttaaa taccgtatgt gtcaggccga gcgggcgata aatcgactct
    58981 tcaacatagc ggtcaagcgg ctgaccggta accgactcga cgataaatcc aagcagcata
    59041 taatcgacat cgctgtagat atgtttgctg ccgggctgat attccagcgg cgtgcgcttg
    59101 atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac cgctttattc
    59161 gggtattgcg gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagg
    59221 gtgtttttgc ctttaatggt gtcatttgga ctatcggcaa accccggaat atatttcgca
    59281 atccgatcgt caggatgcag tttgccttcg ctcatcagct tttgcagggc gaagttcgtg
    59341 gcgtacattt tggtgtttga ggccaggtca tacagcgtcc cggtggtggc tttgacaggc
    59401 tgttccatca acacgctgcc atcgtacttt ttcgccgctc cccaggcttt gcgatacaca
    59461 atctgattat ctttaatgat cagcaggttt acgccgggat aaccgacatc aatttgctgg
    59521 ctaatccagc gatccatctg gttaagccgt tcgacgttaa accctgcttt ctctggcgag
    59581 ctttctgtca gaacagggta ttttgcggcg ctgacgctac aactaacagc cagcagagaa
    59641 agatagagca ttgtccgttt catgatatca gtccagattg acgttacggc agccaaagag
    59701 cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgccagcat aaaccgccat
    59761 ggccggaagg atgccttgtg cggaagtcat cagcggcagc gctaccagac cagacggccc
    59821 aaaggcgctg tttaagccca tcggcagacc ccatcaggct atcaggccga taaacaaacc
    59881 gcccgccgcg ccgcctaaac aggcggtaac aaacggcttc atgcggggca gggtgacacc
    59941 gtaaatcagc ggttcaccaa cgcccagcag gccgggaata atcgccccgc gtacctgact
    60001 gcgtagcgca ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc
    60061 cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctagctgt ccattaacgc
    60121 gaggtaaaca ggaataaagc cctgatgcac gccaaatacc acggcgatca ggaacagccc
    60181 ggctaaaacc gcacaaccga acggattact gttcaggtgc ataaacagcc atgacatacc
    60241 ttcgaataac cagccaccca gcggcataat gatcaggtac gcaagcgtag cggtgatcag
    60301 cagggtgatt aacgaggtca gcagcatgtc gagatcgtcc gacataaagc ggcgcaccat
    60361 gccttcaatg cgggcgcagg cccacgcggc aatcagcacg ccgataatat tgccgcgcgg
    60421 atcgatgggc agaccaaaga aatcgtgaaa accggcgtag tcaccggtgg ttgcagcggg
    60481 gttataaccg agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa
    60541 tgcctgggcg gcgttatagc ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa
    60601 caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat
    60661 caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa aaccggggat
    60721 cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg aggtttgttt
    60781 ggcttttaac tgacgtttgt tctgggcggc gatttctgcg gcatcctgta ccggtgcctc
    60841 gcccagcagc tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc caaaaacgac
    60901 ctgcacctgg tcgctggtca aaatgacgcc cttcacgcct tccagcgttt tgatattggg
    60961 atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt taccacaact
    61021 ggcgatattt cccagtccgc cgacacgggt aagaatggtg ttcagaagtt cactgctgat
    61081 ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc cgtgttgatc
    61141 caggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa tggccgtttt
    61201 acagttgcgc tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac atccggtggc
    61261 gtttttaacg atattgacct ggcgcacatg cagtttttcg ttggtggcga ccacatcgac
    61321 catcaggttg ccgaacactt tgccggattt aatcatcagc ccggtggaaa gcatattgag
    61381 caccagtttc tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc
    61441 tacaatcggt gtaatggcaa actcggcggt ggtggaaacg gcactccccg gattacagga
    61501 aattcccact gtgcggcaac cgagctggcg ggcgtattcc agtccggcaa tcacatacgg
    61561 cgtacgaccg ctggcggcaa taccgaccac cacatcctgt gccgttaaat ttatattttt
    61621 cagatcgtta atgccgcctt ctcggctatc ttccgtgcct tccaccgcgt gctgaatggc
    61681 atattcgccg ccagcaatca aaccaaccac cagacccggt ttcacgccgt aggtgggcgg
    61741 acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga gataaatcag
    61801 acgaccgccg ccgcttacct gggcgtggat aacatcgatc gccgcggcga tatccggcag
    61861 gacgcgctca acggcaagcg gtacggtttt atcttcatcg ttgataatcc ggcacatttc
    61921 cagcgtcgat acgcggtcaa tttcagccga ggcggtgttc gagccttcag taatcatctt
    61981 ttcaagttgc atcggtggtg accttactat ttacgatcaa ataattgcag atgtatggaa
    62041 tcatatatcc cgaattgaca gacttaaagg aatcattgag tctacacttg cagagtgtga
    62101 agagcgtcat aagaacttaa gcggaatttt tgctgccagc ccgggagagt ggaagatgtt
    62161 gtacctgacg aaaatacgca atgcggaaag tgaatttacc gagaacgagc aaaaaattgc
    62221 tgactttctg cgggccaacg tgagtgagct gaaatccgtc tcttcgcgca agatggcgaa
    62281 acagctcggc atcagtcagt cgagcattgt gaaatttgcc cagaagctcg gcgcgcaagg
    62341 atttactgaa ttgcgtatgg cgttaattgg cgaatacagt gccagccggg agaaaacgaa
    62401 tgccacggcg ctgcacctgc acagttcgat taccagtgat gattcgctgg aagtgattgc
    62461 ccgcaaactg aatcgcgaaa aagagctggc gctggagcaa acctgtgcgt tgttcgacta
    62521 cgctcggctg caaaaaatca ttgaggtcat cagtaaagca ccgtttatcc agataacggg
    62581 cctgggcggg tcggcgctgg tggggcgcga tctgtcgttc aaactgatga aaattggcta
    62641 tcgcgtggcc tatgaagccg acacacacgt acaagccacc gtttcccagg cgttgaaaaa
    62701 aggcgatgta caaatcgcta tctcttacag cggcagtaaa aaagagattg tcttgtgcgc
    62761 agaagcggca cgaaagcagg gggcaacggt gattgccatt acttctctgg ctgactctcc
    62821 gctgcggcgg ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag
    62881 ctcgtcgatg tctacccgca cggcgcaaaa ttctgttacc gatttactgt ttgttggtct
    62941 tgtgcaactc aatgatgtgg agtcattgaa aatgattcag cgcagcagtg agctaacaca
    63001 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
    63061 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
    63121 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
    63181 gcattgcagg gaattggcga aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta
    63241 atcttgctgg atatctcccc tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt
    63301 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
    63361 aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt
    63421 cgtctgggca gtttcctcga tatgagcgat gacgatcgcg atttccatat tgacatcaat
    63481 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat
    63541 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
    63601 gcgtacgcct taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac
    63661 gcgcagtctg gtattcgcgt taacgccatt tgcccgggat acgtgcgcac accaatggcg
    63721 gaaagcattg cccgccagtc gaacccggaa gatccagagt cggtgctgac tgaaatggcg
    63781 aaagcaatcc cgctgcgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    63841 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    63901 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatttg
    63961 tggggaggat ttcgttttaa acccggttct tattcccttt tcaacttcca aatcaccaaa
    64021 cggtatataa aaccgttact cctttcacgt ccgttataaa tatgatggct attagaaagt
    64081 cattaaattt ataagggtgc gcaatggccg ttaacttact gaaaaagaac tcactcgcgc
    64141 tggtcgcttc tctgctgctg gcgggccatg tacaggcaac ggaactgctg aacagttctt
    64201 atgacgtctc ccgcgagctg tttgccgccc tgaacccgcc gtttgagcaa caatggacca
    64261 aagaaaacgg cggcgacaaa ctgacaataa aacaatctca tgccgggtca tcaaaacagg
    64321 cgctggcaat tttgcagggc ttaaaagcag acgttgtcac ttataaccag gtgactgacg
    64381 tacaaatcct gcatgacaaa ggcaagctga tcccggcgga ctggcaaacg cgcctgccaa
    64441 ataatagctc gccattctac tccaccatgg gcttcctggt gcgtaagggc aacccgaaga
    64501 atatccacga ctggaacgac ctggtgcgct ccgatgtgaa gctgattttc ccgaacccga
    64561 aaacgtcggg taacgcgcgt tacacctatc tggcggcatg gggcgcagcg gataaagctg
    64621 acggtggcga taaagccaaa accgaacagt ttatgaccca gttcctgaaa aacgttgaag
    64681 tgttcgatac tggcggtcgt ggcgcgacca ccacttttgc cgagcgcggc ctgggcgatg
    64741 tgctgatcag cttcgagtcg gaagtgaaca acatccgtaa acagtatgaa gcgcagggtt
    64801 ttgaagtggt gattccgaaa accaacattc tggcggaatt cccggtggcg tgggttgata
    64861 aaaacgtgca ggccaacggt acggaaaaag ccgccaaagc ctaccttaac tggctctaca
    64921 gcccgcaggc gcaaaccatc atcaccgact attactaccg cgtgaataac ccggaagtca
    64981 tggataaact gaaagacaaa ttcccgcaga ccgagctgtt ccgcgtggaa gacaaatttg
    65041 gctcctggcc ggaagtgatg aaaacccact tcaccagcgg cggcgagtta gacaagctgt
    65101 tagcggcggg gcgtaattaa tgtttgcagt ttcatcaaga cgcgtgctgc cgggctttac
    65161 cttaagcctc ggcaccagtc tgctgtttgt gtgcctgatt ttgctgctgc cgctctccgc
    65221 gctggtgatg cagctggctc agatgagctg ggcgcagtac tgggaggtga tcaccaaccc
    65281 gcaggtagta gcggcctaca aagtaacgct gctgtcagcg tttgtggcat cgatttttaa
    65341 cggcgttttt ggcctgctga tggcgtggat cttaacccgc tatcgctttc cgggccgcac
    65401 gctgcttgat gcgctgatgg atttaccctt tgcgctgcca acggcagtcg ccggtttaac
    65461 gctagcctcg ctcttttctg taaacggttt ttacggtgaa tggctggcga agtttgatat
    65521 caaagtcacc tatacctggc tggggattgc ggtggcgatg gcctttacca gcattccgtt
    65581 tgtggtacgt accgtgcagc cggtgctgga agagttaggc ccggaatatg aagaagcggc
    65641 agaaacgctt ggcgcaacgc gctggcagag tttctgcaaa gtggtgctgc cggaactttc
    65701 tccggcgctg gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg aatttggtgc
    65761 ggtgattttt atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt
    65821 tgtgcgctta caggagtttg attacccggc agcgagcgcg attgcttcgg tgatcctcgc
    65881 ggcatctctg ctgctgctgt tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt
    65941 ggtaggtcat taatggcgga agttacccaa ttgaagcgtt atgacgcgcg cccgattaac
    66001 tggggcaaat ggtttctgat tggcatcggg atgctggtat cggcgttcat cctgctggtg
    66061 ccgatgattt atatcttcgt acaggcgttc agcaaagggc tgatgccggt tttacagaat
    66121 ctggccgatc cggacatgct gcacgccatc tggctgacgg tgatgatcgc gctgattgcc
    66181 gtaccggtaa acctggtgtt cggcattctg ctggcctggc tggtgacgcg ctttaacttc
    66241 cctggacgcc agttactgct gacgctactg gacattccgt ttgccgtatc gccggtggtt
    66301 gccggtctgg tgtatctgct gttctacggc tctaacggcc cgctgggcgg ctggctggat
    66361 gaacataatc tgcaaattat gttctcctgg cccggaatgg tgctggtcac catctttgtg
    66421 acgtgtccgt ttgtggtgcg cgagctggtg ccggtgatgt taagccaggg cagccaggaa
    66481 gacgaagcgg cgattttgct tggcgcgtct ggctggcaga tgtttcgccg cgtcacattg
    66541 ccgaacattc gctgggcgct gctttatggc gtggtgttga ccaacgcccg cgcaattggc
    66601 gagtttggcg cggtgtcggt ggtttccggc tcgattcgcg gcgaaaccct gtcgctgccg
    66661 ttacagattg aattgctgga gcaggactac aacaccgtcg gctcctttac cgctgcggca
    66721 ctgttaacgc tgatggcgat tatcaccctg tttttaaaaa gtatgttgca gtggcgcctg
    66781 gagaatcagg aaaaacgcgc acagcaggag gaacatcatg agcattgaga ttgccaatat
    66841 taagaagtcg tttggtcgca cccaggtgct gaacgatatc tcactggata ttccttcagg
    66901 tcagatggtc gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc tgcgcattat
    66961 cgccgggctg gagcatcaaa ccagcgggca tattcgcttc cacggcaccg acgtcagccg
    67021 cctgcacgcg cgcgatcgca aggtcggttt cgtgttccag cattacgcgc tgttccgcca
    67081 tatgacggta ttcgacaata tcgcttttgg cctgacggtg ctgccgcgtc gcgatcgccc
    67141 gaatgccgca gccatcaaag cgaaagtgac aaaattgctg gagatggtgc agcttgctca
    67201 tctggcggat cgttatccgg cacagctttc cggcggccag aaacagcgcg tggcgctggc
    67261 gcgcgcgctg gctgtagaac cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga
    67321 tgcgcaggtg cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt
    67381 caccagcgtg ttcgtgaccc acgaccagga agaagcgacg gaagtagctg atcgtgtagt
    67441 ggtgatgagc cagggcaata ttgaacaggc tgatgcgccg gagcaggtat ggcgcgaacc
    67501 agcgacccgt tttgtgctcg aatttatggg cgaagtgaac cgcctgcagg gaaccattcg
    67561 cggcgggcag ttccatgttg gcgcacatcg ctggccgctg ggctacacac ctgcttatca
    67621 ggggccggtg gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct
    67681 cgattcgccg ctgccggtac aggtactgga agccagcccg aaaggtcact acacccaatt
    67741 agtggtgcag ccgctggggt ggtacaacga accgctgacg gtcgtgatgc atggcgacga
    67801 tgccccgcag cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc tgtataacgg
    67861 cgacgagcgt atcgaaaccc gcgatgagga acttgctctc gcacaaagcg cctgataggt
    67921 tgagtgaatg ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta atggcaaggt
    67981 ttgtaacctg tagacctgat aagacgcgca agcgtcgcat caggcaacac cacgtatgga
    68041 tagagatcgt gagtacatta gaacaaacaa taggcaatac gcctctggtg aagttgcagc
    68101 gaatggggcc ggataacggc agtgaagtgt ggttaaaact ggaaggcaat aacccggcag
    68161 gttcggtgaa agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa
    68221 ttaaaccggg tgatgtctta atcgaagcca ccagtggtaa caccggcatt gcgctggcaa
    68281 tgattgccgc gctgaaaggc tatcgcatga aattgctgat gcccgacaac atgagccagg
    68341 aacgccgtgc ggcgatgcgt gcttatggtg cggaactgat tcttgtcacc aaagagcagg
    68401 gcatggaagg tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc
    68461 tcgatcagtt caataatccc gataaccctt acgcccatta caccactacc gggccggaaa
    68521 tctggcagca aaccggcggg cgcattaccc attttgtctc cagcatggga acaaccggca
    68581 ctatcaccgg cgtttcacgc tttatgcgcg aacaatccaa accagtaacg attgttggcc
    68641 tgcagccgga agaaggcagc agtattcccg gcattcgccg ctggcctgcg gaatatctgc
    68701 cggggatttt caacgcttct ctggtggatg aggtgctgga tattcatcag cgcgatgcag
    68761 aaaacaccat gcgcgaactg gcagtgcggg aaggaatatt ctgtggcgtc agctccggcg
    68821 gcgcggttgc cggagcactg cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga
    68881 tcatctgcga tcgtggcgat cgctaccttt ccaccggggt gtttggggaa gagcatttta
    68941 gtcagggggc ggggatttaa gattattgcg caggcaccgt tttatccgtc tgcgcaatta
    69001 aggcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc acaatatacg
    69061 gcttcccggt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa
    69121 tgcggttacg ggtattgccg agcttcagac gcagatgaga aaccgcttca tcgcaggtat
    69181 gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc tccagcatgg
    69241 cgttgatgcg ggcttcttca tgaacagtta acttcgcgta agcggggagt gtcattagta
    69301 gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt tttgtgatag
    69361 ccgatttgac tgccagaatc aggtaaagtt tcggttggaa tacgatgagg atcatcggca
    69421 aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagtag ggcgctgcag
    69481 gcggatatcg tcgccgtgca gtcgcaggta gtgtacggca gcgtaggcaa cagcattgcg
    69541 gtgcctgcta taaaacagaa cggcttgaat gtctttgccg tgccgacggt attgctgagc
    69601 aatacgccgc attatgacac tttctacggt ggtgcgattc cggacgaatg gtttagcggc
    69661 tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccaac ttcgtgctgt aaccacgggc
    69721 tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa
    69781 gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga tagcggaatt
    69841 tatgtcaaac ctgaccttcc cgaggcgtat cgacaatatt tactgccgct ggcgcaagga
    69901 attaccccca atatctttga gctggaaatc ctgaccggta aaaattgccg cgatctcgac
    69961 agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggt ggtgattacc
    70021 agcgcctccg gtaatgaaga aaatcaggag atgcaggtgg tggtggtcag tgccgacagc
    70081 gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg cgacctgttt
    70141 tgtgctcagc ttatcagtgg tttgctgaaa gggaaggcgt taaacgatgc ggtgcaccgt
    70201 gcggggttgc gcgtactgga agtgatgcgc tatacccagc agcatgagag cgatgaattg
    70261 attttgccgc cgctggcgga agcataaaaa atggcgccga tgg
//
LOCUS       DAJTVK010000006        64816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000006 DAJTVK010000000
VERSION     DAJTVK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.006"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="OO372_000431"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="OO372_000431"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6340037.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="OO372_000432"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="OO372_000432"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6340038.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="OO372_000433"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="OO372_000433"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6340039.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="OO372_000434"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="OO372_000434"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR6340040.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="OO372_000435"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="OO372_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR6340041.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /locus_tag="OO372_000436"
     CDS             complement(4809..7043)
                     /locus_tag="OO372_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6340042.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="OO372_000437"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="OO372_000437"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6340043.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="OO372_000438"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="OO372_000438"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6340044.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="OO372_000439"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="OO372_000439"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6340045.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="OO372_000440"
     CDS             complement(12798..14138)
                     /locus_tag="OO372_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6340046.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="OO372_000441"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="OO372_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6340047.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="OO372_000442"
     CDS             complement(15927..16376)
                     /locus_tag="OO372_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6340048.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="OO372_000443"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="OO372_000443"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6340049.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="OO372_000444"
     CDS             complement(17176..17505)
                     /locus_tag="OO372_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6340050.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="OO372_000445"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="OO372_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6340051.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="OO372_000446"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="OO372_000446"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6340052.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="OO372_000447"
                     /gene_synonym="ygdH"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="OO372_000447"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6340053.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="OO372_000448"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="OO372_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6340054.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="OO372_000449"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="OO372_000449"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6340055.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="OO372_000450"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="OO372_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6340056.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="OO372_000451"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="OO372_000451"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6340057.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="OO372_000452"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="OO372_000452"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6340058.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="OO372_000453"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="OO372_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6340059.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="OO372_000454"
                     /pseudo
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="OO372_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="OO372_000455"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="OO372_000455"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR6340060.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="OO372_000456"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="OO372_000456"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6340061.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="OO372_000457"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="OO372_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6340062.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="OO372_000458"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="OO372_000458"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6340063.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="OO372_000459"
     CDS             complement(34677..35072)
                     /locus_tag="OO372_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6340064.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="OO372_000460"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="OO372_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6340065.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="OO372_000461"
     CDS             complement(36359..36586)
                     /locus_tag="OO372_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6340066.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="OO372_000462"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="OO372_000462"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6340067.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="OO372_000463"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="OO372_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6340068.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="OO372_000464"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="OO372_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6340069.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="OO372_000465"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="OO372_000465"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6340070.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="OO372_000466"
     tRNA            40666..40742
                     /locus_tag="OO372_000466"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            40776..40852
                     /locus_tag="OO372_000467"
     tRNA            40776..40852
                     /locus_tag="OO372_000467"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40810..40812,aa:Met,seq:cat)
     gene            40886..40962
                     /locus_tag="OO372_000468"
     tRNA            40886..40962
                     /locus_tag="OO372_000468"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40920..40922,aa:Met,seq:cat)
     gene            complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="OO372_000469"
     CDS             complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="OO372_000469"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6340071.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42522..43853
                     /gene="argA"
                     /locus_tag="OO372_000470"
     CDS             42522..43853
                     /gene="argA"
                     /locus_tag="OO372_000470"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6340072.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43915..45741)
                     /gene="recD"
                     /locus_tag="OO372_000471"
     CDS             complement(43915..45741)
                     /gene="recD"
                     /locus_tag="OO372_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6340073.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45741..49283)
                     /gene="recB"
                     /locus_tag="OO372_000472"
     CDS             complement(45741..49283)
                     /gene="recB"
                     /locus_tag="OO372_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6340074.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="OO372_000473"
     CDS             complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="OO372_000473"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6340075.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52340..55708)
                     /gene="recC"
                     /locus_tag="OO372_000474"
     CDS             complement(52340..55708)
                     /gene="recC"
                     /locus_tag="OO372_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6340076.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55721..56044)
                     /locus_tag="OO372_000475"
     CDS             complement(55721..56044)
                     /locus_tag="OO372_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6340077.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56029..56436)
                     /locus_tag="OO372_000476"
     CDS             complement(56029..56436)
                     /locus_tag="OO372_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6340078.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56433..56996)
                     /locus_tag="OO372_000477"
     CDS             complement(56433..56996)
                     /locus_tag="OO372_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6340079.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56987..57457)
                     /locus_tag="OO372_000478"
     CDS             complement(56987..57457)
                     /locus_tag="OO372_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6340080.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="OO372_000479"
     CDS             complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="OO372_000479"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6340081.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="OO372_000480"
     CDS             complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="OO372_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6340082.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="OO372_000481"
     CDS             complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="OO372_000481"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6340083.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="OO372_000482"
     CDS             complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="OO372_000482"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6340084.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62943..63632
                     /gene="mutH"
                     /locus_tag="OO372_000483"
     CDS             62943..63632
                     /gene="mutH"
                     /locus_tag="OO372_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6340085.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63701..64414
                     /locus_tag="OO372_000484"
     CDS             63701..64414
                     /locus_tag="OO372_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6340086.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64552..64770
                     /gene="ygdR"
                     /locus_tag="OO372_000485"
     CDS             64552..64770
                     /gene="ygdR"
                     /locus_tag="OO372_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6340087.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15710 a        17425 c        15892 g        15789 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ttggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttat
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaagcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgtgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acacgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagtg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccggat tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgccacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaaga
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttccaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagctagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgcg ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tccgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattaaaat ttgatgaagt aaagcagtac ggtgacgcgg ggtggagcag cctggtagct
    40801 cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caatcaaatt
    40861 tgatgaagta aaagcaatac ggtgacgcgg ggtggagcag cctggtagct cgtcgggctc
    40921 ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caattattga acaccctaac
    40981 gggtgttttt ttgtttctac tctcccataa aaaaagcgcc attcagcgcc tttttatcat
    41041 ccccttctcg ccagcgttgc cccatcggca aaatacgctt taatccccgc aagaatagac
    41101 tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gctcttcctc aacgttactg
    41161 ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac ggcaaacccg
    41221 gcctgttcaa cttgattttt atgcagcttg ttgattttac cgagcttatt cagcactgct
    41281 ttaccaaact taaggctgtc agcaatggtc agcgactgca ccatatcgaa catggtgtgg
    41341 tcgacatagc ggtcaccgct tttgctcacg ccaccaatca agtccgaggc gttctgggtt
    41401 tgtgccagat attttgccgc agtactggtt gcacctttgg ttgagagcgc aaacaccgag
    41461 gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga tagagacaaa caggtcagca
    41521 cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc
    41581 gtcatgtaca ccttcatatt gccctctttc tcgatcagag agcgcaaacg gcgagctatt
    41641 tgcaatacca catctttttc gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca
    41701 ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc gccctgcttt acctggttgt
    41761 ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc ctttgttgta atcctccagc
    41821 agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc catcaccaga
    41881 cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac gttttgcttt
    41941 aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg
    42001 aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga gtaccgagtt caggtttaca
    42061 tcttcgatat ccaccaccac gcgttcaggg ttactcaacg cgaactgctt atatttcagc
    42121 tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac gcgcaccgcc
    42181 acgacctggc taaccgcagc caggctgacc tgacttacgc tcaatagcca catagcgccc
    42241 gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat gcctctcccg
    42301 agcaaaagaa atctaagctg tgtaacaagt aaacgactaa tttgaccgga aactttagcg
    42361 aaagacgcat aatctgtcat ctaataaacg gtaaacattc tttttatatt cacggcatta
    42421 ctgataaaaa agtcgctctc gcataaaatt tacacttgca ccctgcgaaa aaacagaata
    42481 aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc cgtggtaaag gaacgtaaaa
    42541 ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac cggggaaaaa
    42601 cgtttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc agtatcgtta
    42661 atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat ggcgcacgtc
    42721 cgcagatcga cgcaaatctg gctgcgcatc accacgaacc gctgtatcac aagaatatac
    42781 gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg
    42841 atattactgc tcgcctgtcg atgagtctca ataacacgcc gctgcagggc gcgcatatca
    42901 acgtcgtcag tggcaatttt attattgccc agccgctggg cgtcgatgac ggcgtggatt
    42961 actgccatag cgggcgtatc cggcggattg atgaagacgc gatccgtcgt caactggaca
    43021 gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt cactggcgag agctttaatc
    43081 tgacctcgga agagattgcc actcaactgg ccatcaaact gaaagctgag aagatgattg
    43141 gtttttgctc ttcacagggc gtcactaatg acgacggtga tattgtctca gaacttttcc
    43201 ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa aggcgattac aactccggta
    43261 cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgt cgctgtcatt
    43321 taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt gttctcacgc gacggtatcg
    43381 gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc aacgatattg
    43441 gcggtattct ggagttgatt cgcccgctgg agcagcaagg tattctggtg cgccgttctc
    43501 gcgagcagct ggagatggaa atcgacaaat tcaccattat tcagctcgat aacacgacta
    43561 ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat tggggaaatg gcctgtgtgg
    43621 cagttcaccc ggattaccgc agttcatcac ggggcgaggt tctgctggaa cgcattgccg
    43681 ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc agtattcact
    43741 ggttccagga acgtggattt accccggtgg atattgattt actgcccgag agcaaaaagc
    43801 agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttaggg taagcgatga
    43861 aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca tccggcaatt acgtttatcc
    43921 ccgcgaacta aacaacgccg ccagaccact gcgccgctca gtacgagtgg cgattgccgc
    43981 acttaatata cgctcatcgg catacagcga cagacggcga cgcgcgcggg tcaccgcggt
    44041 gtataccagc tctcgcgtta ctatcggcgt gcgttggctc ggtaaaatca acgccgcatg
    44101 gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc cacgtagttt cgtgctctgg
    44161 caagcgactc ggttgcacag acttaatatt gccgtccggc atcgcaaacc agacgcgcgt
    44221 cccctgcccg cgatccagcg caataccgat atcgccatta aacaacccaa gcgcgctgtc
    44281 attacgggca atcatcaccg gtcgaccttc gtaccaacga gagtgcgaat gacgatgaat
    44341 tttgcgcttc tgttgcataa actgctcaat tcgctcattc agtccagcca cgccaaatgg
    44401 cccttcccgc agggcgcaca aaagttggta ctcattgaac gcctgaatga ttaaatccgg
    44461 ctcggcacgc gcttgcagca gatccagata acgtccataa cccgcaagag cttcctcaag
    44521 catcgcaata taatcttcgc cgctctgtaa aagccgtttt tcaatatcag taaaatcctg
    44581 ctgaaaaacg gttttcactg ccgttttatc accacggttg atcgccgcag ctaactgacc
    44641 aatgccagaa tcgctgccga aacgatagct tttttgcagc aggcagagac tgtcgcgcaa
    44701 agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt cccgtcaggc ggcttagctg
    44761 cccggcacgc tcggcggtaa agccggcatt ggcataagcg cagatatcgc ccagcacagc
    44821 cccagcctca accgaggcca gttgatcacg atcgccgaga aagatcactc gcgcatgatc
    44881 gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg atcattgacg cttcatctac
    44941 caccagcaca tcaagatgca gcgggttacc ggcatgatga cgtaaacgct ggctacccgg
    45001 ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc ggaatgcgtt tcttttgttc
    45061 atcggtcagc ggtaactgtc gcaaagcctt gccgagagat tcggttaagc gcgcggcagc
    45121 tttacccgtt ggtgcagcca gacggatacg gcagcgttcg ccgtcggcca tttgaattaa
    45181 cgctgccagc aacttcgcca cggtggtcgt tttaccggtg cctgggccgc cggaaatcac
    45241 cgagatccgc cgcgtcagcg ccactgctgc cgccactttt tgccagttaa tttcatcgct
    45301 tactggaaaa agtttgtcca gggtttgcgc cagtagagct tcatcaacct caatggcatg
    45361 attcacctca ttgaagaagc gcgctaccgt ccgctcgtta caccacatgc gattcaaata
    45421 aagacgatcg ccacagagga tcatcggcgt gggttcatct cccctgctga ccgcttgaga
    45481 agccagcaag gattcttccc aattttgtag ctcaccgatt tcactgacac aggtcgccaa
    45541 cagcggatgc gacgcctcgt tattttccag tcgtgaaagc ggcaaacaga cgtgtccctc
    45601 tccggcatca tgacttaaca gtgccgccgc gagggtgacg gcaggatgtt catctcccgc
    45661 cacggtcagg gcaaattgca catccagcgg gcgtagctgt ttgtgctcca cagcttccag
    45721 taattgcttt tgcaatttca ttacgcctcc tccagggtca taccggcaaa catctcatcc
    45781 atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa tcccttgttg cggatgttct
    45841 ttatcaacgc cacgcaggaa cagatagata acgccgccaa agtgacgatc atagtcgtaa
    45901 tcagcaatgc gatggcgcag atagcgatgc agcgccaggg tataaagctg atattgcaga
    45961 tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt gggtgtaagc cgaactgtct
    46021 tcacccagcc agttggattt atagtcgagc aggtaataac gcccttcgtg gcggaatacc
    46081 aggtcgataa agccttttaa catgccgcgt acctgcatga actccagcgg cgggcagcca
    46141 gcggatagcg ggtcaaactg gcgaattagt gcatcaagct ggctggcgat gagcggttcg
    46201 ctaatcggca ggtaaaactc catctccacc tgtttatcgc ggtcggaaag ctgactcagg
    46261 ctaacacccg tttcattgag aggtgcctgg aggacagccg tgatccactc ggtcaacacc
    46321 ggttcccact gcgattcaaa gccgccgagt tccagttttt cctgtaccca gttcgggtca
    46381 accggctggg taaaatcgag gtcttcaaac aaactgtgca agaatgtccc cggtgacgca
    46441 ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa cgacgctgac cacgcctgcg
    46501 gcatcaacat ccagccgtgg catcagatcc tgagcgatac cgtgaccacg ctgctgcaag
    46561 ccggagtagc tggtgacacg ccagttatcg ccgggcaatc gttgtagtgt cctcgcattc
    46621 agttctgcag ttaaagcatc attaacctgc cagggctggt tatcaccagt ttgtgccgtt
    46681 tgccaggcaa tatcatcatc gcataacgct tcaatacagg tgcgaagccc tgccgcatct
    46741 tgcggttccc ctttttgcag caaacgcccg agcgcacttt ggtggacgtc ggtgtcacct
    46801 tttttatcgc cacgacggcg caccagcggt gcaacgccga gactgcaatg ccaaaccgaa
    46861 cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca gacgttcgac ctccgcgagg
    46921 tcgacgcttt ctggcgcagc attaagatcc aaaactgcct caaacgagtg gcgatcgtga
    46981 taaaacgcct gatcctggac gcggaaattg gtaataaatg gcaaccagac cagcggatat
    47041 tccagccctt tcgatttatg gatcgtgaca atctgcacca gatgtttatc actttcgagg
    47101 cgcatttgtt ggctggaggc attactgtct ggctcgagga tatgttgcga taaccagcgt
    47161 accagcgcat gttcactttc cagctgcgtt ccggcttctt gaagcagttc gctgatatgc
    47221 aagatatcgg taagacgccg ctcaccgcct gccgttgcca gcaagttttc tgcaatgtta
    47281 cgcgccgaca tcagcgcccg cagcatcggc ataacgccac gtttgcgcca gatttgccga
    47341 taaccatcga actcttcgac taccgcatcc cacgcatgtt cgtcattatt cagcgtttca
    47401 atatccagcg cattcagccc catcattgac gttgccagcg cactgcgcag ggtgttctca
    47461 cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt cctgcgcttc cagagtttca
    47521 aaaacactat cgcggttcga aaggtaaacg gaagggattt ccagcaacgt taaggcatcg
    47581 cgcacctggg cggcctcctg gcggctgcgc accagcacgc tgatgtccga agcacgcacc
    47641 ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc gctgtccggc ttgtagccag
    47701 tcgcggattt gcgcagcaca tacctgcgcc atggtacttt gatagtcgcc aacgccgcag
    47761 ctttcgcctt ccatcagcca cattttcatc gcaggctgcg tttcaccttt aaagacaaaa
    47821 cgtaacgcct gatttttccc ggctgatttc actggaataa acggtatttc gcgaaacatg
    47881 aacgcgtcat cagtctggct gaaaagcttg ttcacactgt tcaccattcc aggtgcggag
    47941 cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac tacgcgcctt catataagtg
    48001 aagatatccg caccccggaa tgcatatatg gcctgcttcg ggtcgccaat cagcaacaat
    48061 gcggtttctg gctgatggtg ccagatacgg cgaaaaattc ggtactgctg ggggtcggta
    48121 tcctgaaatt catcgatcat tgccaccggg aatcgcgtac ggatcgccgc tgccaacacc
    48181 tcgccgcttt cgctacgcag cgcggaatcg agccgactta acatgtcatc aaaacccaat
    48241 tcgccacggc ggcgtttttc acgcgctact gtttcgcgga tctcagccaa tgcgcgggtg
    48301 atcaccagat cacggatcga caatggttct gcaagcagtt gatcgatcgc ctcaaacagt
    48361 ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt ctaagaaacg ctgggagaat
    48421 ttttccagcg actccggcaa ctgataactg tttgtctctt cttctgccca ggcgctgatc
    48481 ttgtcgatcc atttagcctg attgctacgg ttaaacttgc gtcgatcaat accagaagat
    48541 tcgatcagcg catccagttc acccactgcg tcgcgccact gctgttttac cgtatcaata
    48601 cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat catcgggcgg cggtgctttg
    48661 ataaccggcg cttcgccttg cagataacga ttaatatcgc gcagcaacgc ctgcggccct
    48721 ttccaggttt caaagacgac ctgggctatt tcacgcggca gcgggtagca gtggcgacgc
    48781 cagaaatcgg cgcaggcctg gtagcgtagc agagactcat cttcaatcag ctgctgctca
    48841 aacagcatgc cggattcaaa ggcattcagg ttgagcatgc gctggcaaaa gccgtgaata
    48901 gtaaagactg ccgcttcatc catctgccgt tcggctaaca acaaccactg cgcggcttgc
    48961 gctttatcgt cgacctcttc cagcaggtgt tcgtacaatg gattgtcggt ggtttcacgc
    49021 agacaggcga tgcgcaactc gtggatattg ctacggatac gaccgcgcaa ttctgccgtg
    49081 gcagcctcgg taaaggtgac caccagcagt tcttcaacgg tcagcgggcg gggaaaggcg
    49141 gcggaaccgc ctagtccaag taacaggcgc aaataaagcg ccgcaatcgt aaaggttttg
    49201 cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta agggcaagcg caaaggatct
    49261 agtgtctcgg cgacatcact cattcttttc actcatcagg ggcattgttt gctgcaacgc
    49321 gctgacgttc tcccacactt tccagccttc aggatgcaca tattcggctt tcccgttttg
    49381 gctgccggaa atctgcgaca ggatagccat gccttgcggc tcgactaccg cctgatggaa
    49441 gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg gccacgattt tatcacgcga
    49501 atcgaagcgc atattgccgc gatcgaaatc tttacttaac ttcgatgctt cttcgccgag
    49561 cgtttgcggt gcctgcagca tctgggtaat taccgcctgc tggatttgcg caaactcatc
    49621 tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa aacgccttgt aacgctccca
    49681 caagaatgaa ggctgtttat cattgctttg caaaaggaag cccatgcccc actgacgccc
    49741 cacgctcatt ggaaacgcaa acacggcata gcccaattgt tcttcggtac gcaactgatt
    49801 gtagaaccac ggctgtacga tctgccccaa cagagagcta taggctgagc tggtgtattc
    49861 atcgtagcca gtcggtacaa ataccgctgc cagtgcggag tcggtgctgt taccggcttt
    49921 ttcaaagatg acggattgtt ttttatcgac cactacatct ttgtttcgac accactctga
    49981 accatcagcg cccaactgtt tttgcacatc gcgtgccagc gttgttgcct gggcctcggt
    50041 catgttgccg ataaccataa actctggtcg agcccctgat tttaaggcgt cgcgataagc
    50101 cagcacctct ttcaacgtaa tggagggcaa aattttacgc cgttcatctc gcgagaagta
    50161 cggcacttgc gagagcatct gcgcgggcat aatcgcctgc tcaaacgctt tacccttttc
    50221 tgcggaatcc atcatctggt tataccagga cttcgcctgc tcaagctgat cttccgtagc
    50281 ggtatagcta aagtacccct cgagcaatgc ctggaacagc tgcggcagac gctgggtgta
    50341 accattagca ttaaccataa ggccgttgtt agcgttggtg gaaaaactta tgccaccaac
    50401 cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg agataatcat tgagcgcaaa
    50461 catcacctga ttgcgggcgc tgtccatggc tttcggatta cgcaaaatca ggctgacatc
    50521 agctttgggt tcgctggaaa aataacggct tggcgcatac accacgcgca gattcgactc
    50581 atcaacaatc agctctggat ggtcatattt cttctctgac ttaatcagcg agaaatcgtc
    50641 aggaatatag gggttaagct ccggtaagga gagcgcaata ttggcagctt tttgctgcca
    50701 gtcagcgaaa gtttgttcgc tgattttatc gacctgatac ggcgcatcga caaaataagc
    50761 cgttttattg tgcggctctt tcgggctgat ataccagata cgcgcattct gcggcgtcat
    50821 catcgccaga cgttctttta ctgctttagc atcgtaccga tcggcaatat tgactgcatc
    50881 cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc agccattcga cgtaatccat
    50941 atcacgggtg atcgacggat aacggaagtc gatatccagc acattcgcca gttcatcgaa
    51001 gtattgttta tcaatgcctt tttcacgtaa cagattgaga tagctaaaaa ttgccgccac
    51061 aacctgatcg cgattagcca ggcctttatc ggttaaagac gcagagatcg ctaatacgcc
    51121 gctgttgccg ttgacgatag gatcggagtt ggcgctaatg ccctcaacta atccctgctt
    51181 ttgcagccag tcggaaagtg tacctgggct gcgattacca atcagatagg taatcaattc
    51241 atccgtttta ctacggaact tcgctgagtt gttatcgatg cgaaactcaa cgcgcagaac
    51301 tttacgcggc attgccggga cgtaatgaat gataatgccc ttttgcgcgt cggtgactac
    51361 cggcacggtg atttccggtt ttttgctctc tttgttcggc acgcgaccaa aggtgtccgc
    51421 cgccattttt gccaactccg gcagcggttt attgctgtaa ataaccgcct tcatcagatt
    51481 ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc tgcaccggat taccaggttt
    51541 atcgcttaaa gtttcgaggt taccaccaga aaactttgaa ccggggtgtg ccgggttaat
    51601 ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc gtacgcgcca tggttaattc
    51661 agcgttcacc gcattacgct cacgttcggc atatttcttg tcgagcaaag gttcagcaat
    51721 agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg ttctcaactt ccagatagaa
    51781 agccgtgcga tacggcgcag tgctggcatt gtgactaccg ccgtgcattt tgagatattc
    51841 ggccagactg tcagcctgcg ggtacttttt cgaccccatc agactcatat gttcaaggta
    51901 atgtgccagc ccctggtacg cctcgggatc ttccagcgac ccaacgggca ccaccagcgc
    51961 cgagagcgat ttaactgcct gcggatcaga aaccagcaag accaccatac cgttatccag
    52021 acgtatagcc tgatactggc ggttatcttt atcactttta cggatggttt cctgaatcgg
    52081 ctgccatccc gtttctgcct gacttaaggg tgcccaaagg gcaactaaca acaataatgc
    52141 tttgaaccag gtgctgcggg gcattctcgg acctcataat caacttaatt ttctgtccag
    52201 attcaacacg ttaacgcaaa tcatctgcca gaatttaatc ttgtgctgca cgagtcagcc
    52261 tatgtttata taaccatcag tccgtgactg gtgcgcatca taaagtaagc ggatagattg
    52321 cgcaattttt atacagcact catgactgat taaagcgaaa cagcggtaac aggaaacgtt
    52381 gcgactgttc aacgatggcc tccattgtct ctggtgttaa ttgccgccag agcctttgat
    52441 accagatatc atcaccttcg ccacgcacca tcatgttacc ttcgtaagcc tgaaggaatt
    52501 tcgtacgggc tttttgcaac gtggaatcgt catccagcat ggcatcgttt tgcgcgtcat
    52561 aacaggtttt tagccacgcg ccgccacttt caggtaacac cagcaatggc gcggacatcc
    52621 cttcacgata cccctcaatc agttgtgaga ggtaatgcaa agcctgttcg gctgcaagcg
    52681 gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg actttcacca ttaccaccgc
    52741 tggcacagta gacaaggtgt tccagccaaa gttgcattcc ctgcgccaca cttaataaag
    52801 agggacgcca gcgcaacagg ccatccggct gcacctgcgg caaccagcca gttatctgca
    52861 caccgttgca ggcgagatca atttccatac tctgccccgg ctggcgacag gcaatgactc
    52921 tgtcggcaag ctgctgcatc tcctggcact gagtttccca gaaaatttca ccaaaagcgc
    52981 catacggtaa atcccctgcc gcccggaagc gacggaacaa gcgttcggca tcatcctgct
    53041 caaccagtgc attcaataac tgctgattga tttgataacg gctaagccct tccagaataa
    53101 atggctcggt gtcggggatt tcgctgtctt cagtacggaa gttcacctgc aaacgcatct
    53161 ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag cgtttccagc ggcacggttt
    53221 ccggtaaggt aaacggcagc ggctgaacaa attcagaatg tgctttacca gcctggctgg
    53281 ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc tggctgatag ttttgtggat
    53341 caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt tacccttgcc tcgctttcat
    53401 cacagttgag cgcttcatcg cccggtagat aatgactttg cccgatgtag tcgatcagtt
    53461 cctgcaccag taccgacggg aaacgttcac tgttatcctg aatggaacgc ccgatatagc
    53521 tgatatagag tttttgctgc gcggaaatta acgcttccag gaacagatag cggtcgtcat
    53581 cgcgacggct acggtcgcca cgcttcggtt tctggctcat caggtcaaag cccaatggcg
    53641 caagctgacg tggataaacg ccgtcgttca ttcccagcag gcaaaccact ttgaacggaa
    53701 ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc ggcgagaaaa cgctggctga
    53761 tacgttcttg atccagacgc tgtgccagtt catcacgcaa tagtgacagc ggcaccgcgt
    53821 cgccatactg cgcacctaaa ccttcggcga taatcgcctg ccattgttgt tcgatcagcg
    53881 tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc gctgagcata tcgcgacaaa
    53941 ccggcaacca ctcttctagc ggacgctcct gtgccagccc gcgacgccag atgtttagct
    54001 gcattagcag tgaagccaga tgccccacca gttctgcaat taagccgctc gattcatcat
    54061 aaggtagaac cgattgccac tcgccctgcg cgctctccat cgcgtagccc agcaacatac
    54121 gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg gagttccagc tcgcgaacgt
    54181 tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac ccactggcgt aaataacgca
    54241 gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg cacatccagt aatgccagca
    54301 cgtcttccga cacaaagcgg ctgtctggca acgataacag gctgataaac gcttccagta
    54361 ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta aggcaggtaa cgatccgcag
    54421 gtgcgctacc aaacacagcc cgaataaacg gactgtagct gtcgatatca gccaccatca
    54481 cgatgatgtc gcgcggagta agtgtcgggt cttcttccag catcgccagc aggcgatcgt
    54541 gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg gaaggtgata ctgctatcca
    54601 gtggatcaag cgggcgtttg ttatcgctac gggaaaactc ttcgatgttc acaccagcaa
    54661 cggcacggtt ttccagttcc agaatgtcag actgaatgtt atgcagcagg ttatctggcg
    54721 tgacatcgac aaaggcgtcc agctcctggc tgctctccag gtcagaaagg agataaatgt
    54781 agtcgcgccc aagcttaccc catgaagcca gcagcgggtt gccgacatcc tgttcaccat
    54841 cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa tagcggcaat tcgcgatctt
    54901 caaaactgtg tcgacgctga cgggtcagca gtttcgccag ataagcagga tctttaatat
    54961 cgccccagta ataacggcag gggttggtaa acaggagatg gatttcaata tgtttaccca
    55021 gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga aataccgcat ataaagacgc
    55081 gcgaaggtaa ccccggcggg caggtcgtcg cggactccag cgtttcgata aagcgctgat
    55141 agagattggc gcggtgccag cgcggttgcc cgagttgatg ggtatattcc accagcgcct
    55201 tccacaacgg ggcttgccag gcctgtgctt ctcccagccc ttcaaccaaa tgtcctgttt
    55261 cccactgtgc cagccagtcc ggacgataga ccagatactg gtcaaacagg tccgccgctt
    55321 ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc agtcagataa tgccgcaaca
    55381 gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt catcagtttc cagctcatgc
    55441 tctgtttgtt aaaggcgctc tctttgggga tttccggtaa cacccggacg aacatatccc
    55501 agataaagct cgctggcagc ggaaaatcaa tgtttgccgc aatactaaac ttttgcgaca
    55561 gggtcatttg cagccactgc gccataccgg tactttgcac cagaatcatc tctggttcga
    55621 aaggatcgtc cagccgttcg cgttcgacaa taaactccat caacgcttcc agcacgtcca
    55681 gacgattgga atggtagacc cttaacatag cggctcctga ctactgacga ttcgggcaat
    55741 gcaggcgggt catctcgcct tctctgcccc cgggtgaaac tagcgtaacg ctgatgctga
    55801 cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca gttggcaggt ggcgaaatcg
    55861 cgcgcagttg cgtttgctgc caaccatgcc gccagagctg ttggtactgg tttctgctgg
    55921 cgagactgtt cattaatgtt cgctggatac ccgataacgc agtgacaatc atcaccatca
    55981 gcaccatcgc aaccattacc tccggcaggc taaagccttg ttgattcttc agggaagctg
    56041 acataacgcc ctctctttca gcggacaaaa atcgctccag ccgcgtggcg aaaaaacaat
    56101 gtttccatca atgacttcgc ctgttcgcca caacgaaacg ccttcataac cggcaaccaa
    56161 taaggcttca ttatctgcca gtaaacgcaa acaaacccgg gcatcggttc cagcgtactg
    56221 cgagcactga actgcgggct gcgtctgcca ggagtgcatt ttcccccacg ccagcgccga
    56281 ctgaacgatg gcctggcggc gcaatgactg actttctatg ctcacgcgag aggcaaaact
    56341 gcgatcctgc tgactcattc cttgcaatag caagctaccc aaaaccagca gcatcaggac
    56401 cagagccagt gacgaaacac ctttttcgcg gttcacaggt tgaatcctgt cacgctatag
    56461 ctggcatcca ccacggtttg cggttcagac ttactggcag cacgcatatt aaccgtcaac
    56521 accggcgaga agccgctgac atcctgacgt acgacctgaa aagtgtcgat aatgatggca
    56581 tccggattag tgactttatc ccagccctta ccttcacagg atgtcgcacc gcgtagcgtt
    56641 tccagcacat gctccttcag acgaaatcca atctggtcag actcttttac cggttcgcga
    56701 tcccagatac cgttactgtt cgcatcccac tgcacaatga tacagtcacc ctgtccgaca
    56761 atttccagcc cttcgccggt acagctgcca tgacaataac ccgccctctg gagatgcttc
    56821 gcgacggtaa atacccgcag ccagatttca tcttccagcg ccagcttacg ggtgctcgtt
    56881 aaactttcac gctgtaacgc aggcagaaag cgtgcagccc ccagcaacaa tacgctactg
    56941 atcgccatag caatcaacac ttccagcaga gaaaaacctt gctcttttac aggcatcctt
    57001 ctgtttctcc ttgctgacaa agccggagtc ttccccacgg cgaaaccacc agccaccact
    57061 cgcccgttga gtttttgaag cgaatatgcc cggcccatgc ggtattgcgc aggccaaaga
    57121 aagcaagcga aggtgtcagg tcgctcattt cgacttcggg ccagcgtggc acaaagacca
    57181 atggtgaact gccatgacag gtattggccc cagcagcgga actcacaagg caccataacg
    57241 tcccctccct gataacgctg atactgtggt cgcggttatg ccagttggca tcttcacgta
    57301 aatagagcaa atagtcccgc gcctggctgg cggtttgcca tagccgttgc gactgctgcc
    57361 agtattgcca gccatagagt ccacttgcgc ttagcatgac caaaatcagc atcgcgacca
    57421 gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc ggcagtatgg agcgaggaga
    57481 aaaaaagacg agggccagtt tctatttctt cggcgcatct tccggactat ttacgccgtt
    57541 gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat ttaacggagg gtaaaaaaaa
    57601 ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct
    57661 ttaatgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag
    57721 atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat
    57781 tgcagatgag tttgatccat atgattgctg tacaggtgcg tatcgccacc ggtccagaca
    57841 aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg
    57901 gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag
    57961 agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta
    58021 tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gggaatccgg gtcgtttttc
    58081 agctggttca gtaccgtagt gatctggtca atatgacgac catctggcgt tggccaggcg
    58141 cgccactgtt taccatacac tggtccgagg tcgccgtttt catcggccca ttcgtcccag
    58201 atggtgacat tgttttcgtg tagataagca atgttagtgt cgccctgcag aaaccacagc
    58261 agttcgtgga tgatggaacg caggtggcaa cgtttagttg tcaccagcgg gaatccatcc
    58321 tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg
    58381 tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt
    58441 cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc
    58501 gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg
    58561 gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca
    58621 atcaggaaca agccagagac agctcccatt gggcgcggtt tacgaatata caggttgagg
    58681 ataataaaca gcaccacacc ttccagcagc agctcgtaaa gctgtgatgg gtggcgcggc
    58741 agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata
    58801 tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc
    58861 cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt
    58921 ggtgcgataa aatcagaaac ctggaagaag gaacgtttag tacggcgggc aaagataatc
    58981 atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg
    59041 aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata
    59101 cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg
    59161 gtccagccgc tgcccggacg attcgcccgt cgtgttgcca gccacattgc aaaaatgaaa
    59221 cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc
    59281 ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta
    59341 tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt
    59401 gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat
    59461 gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct
    59521 gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt
    59581 tctgcttcgg cataatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg
    59641 ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc
    59701 gccatttcac cgcacaaacg gagatcgatt ccatgtattt ccgcttcccg ggcgatcatc
    59761 gccagagctc gtaacattgc aggatgaaga ctgtcataaa tgttcgccac ccgggtattg
    59821 ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg
    59881 acccgctttg ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg
    59941 atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca
    60001 atcaggcggc gtgcttcatc aacttcatcg aggcttgtga ccatcggcaa cagaatattc
    60061 aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc
    60121 ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc
    60181 ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta
    60241 tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa
    60301 ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacggctg
    60361 cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca
    60421 ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc
    60481 tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat
    60541 tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc
    60601 gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct
    60661 gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc
    60721 gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta
    60781 gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcgcc agcccgctct
    60841 ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt
    60901 accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg
    60961 gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg
    61021 gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc
    61081 agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa
    61141 ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg
    61201 cggattcgcg tctggcgata ttgcccaaac aaggcagtca actgcgactg agaaagaata
    61261 gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc
    61321 aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg
    61381 cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc
    61441 ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct
    61501 tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc
    61561 aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc
    61621 gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct
    61681 accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta
    61741 cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca
    61801 ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg
    61861 accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc
    61921 atattgattt ctgcatcgcc gctcaccagc tgcaagagaa accatttttg tttttggccg
    61981 atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag
    62041 ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat
    62101 tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc
    62161 caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg
    62221 atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct
    62281 ttatatacga atgattgttt catactccaa gaagacggta aaccactctc tgcagggcat
    62341 tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg
    62401 ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt
    62461 actacgggaa aacccggagc attctgaata agcccgatat agacatttta aaatattcat
    62521 tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt
    62581 cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg
    62641 tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt
    62701 aaatggaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt
    62761 gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac
    62821 tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa
    62881 aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg
    62941 acatgtccca acctcgccca ctcctctctc ctcccgaaac tgaagaacag ttgttagcgc
    63001 aagcacagca actttctggt tatacattgg gagaactggc ggcacttgcc gggctggtta
    63061 cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag
    63121 gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa
    63181 ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt
    63241 taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg
    63301 tactgtggat accggttgaa ggtgagcgca gcatcccgct ggcgcagcgt cgcgtaggat
    63361 caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag
    63421 aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggagt
    63481 atttacagat acgaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc
    63541 ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg
    63601 cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg
    63661 catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac
    63721 cgatcctaac gcctggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat
    63781 cgacaatatt attttccttt ctctggtggt ggcaaagctc cccacagcac aacgtgctca
    63841 tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat
    63901 cgcctgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttccgc
    63961 ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat
    64021 ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg
    64081 cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc
    64141 tgtgggtctg tcagatcacc tgtttattat gatggccgcc gtggtgattg ccgtaggcgt
    64201 gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat
    64261 gctggcgctc tctttcctga ttctggtcgg ctttaccctg attctggaaa gtttcgacat
    64321 ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct
    64381 caacctgatt cgcaacaaaa agaacccgct ctgatactcc gtacgctctc ctgcgggagg
    64441 gcgtactcac tcctgttacc ctcacagttt aagattttcc tgctttcaag atatataacg
    64501 tcggtttata aacagactat tatcataggt gggccaaatg aggataaccg gatgaaaaaa
    64561 tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat
    64621 tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat
    64681 gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt
    64741 gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctgtaatgcc
    64801 gatcagttaa ggatca
//
LOCUS       DAJTVK010000007        63231 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000007 DAJTVK010000000
VERSION     DAJTVK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.007"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..415)
                     /locus_tag="OO372_000486"
                     /pseudo
     CDS             complement(1..415)
                     /locus_tag="OO372_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(521..3082)
                     /gene="mutS"
                     /locus_tag="OO372_000487"
     CDS             complement(521..3082)
                     /gene="mutS"
                     /locus_tag="OO372_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6340088.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3158..3292
                     /locus_tag="OO372_000488"
     CDS             3158..3292
                     /locus_tag="OO372_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340089.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3369..3722
                     /locus_tag="OO372_000489"
     CDS             3369..3722
                     /locus_tag="OO372_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6340090.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3759..5836)
                     /gene="flhA"
                     /locus_tag="OO372_000490"
                     /pseudo
     CDS             complement(3759..5836)
                     /gene="flhA"
                     /locus_tag="OO372_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5910..6920)
                     /gene="hypE"
                     /locus_tag="OO372_000491"
     CDS             complement(5910..6920)
                     /gene="hypE"
                     /locus_tag="OO372_000491"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6340091.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6917..8038)
                     /gene="hypD"
                     /locus_tag="OO372_000492"
     CDS             complement(6917..8038)
                     /gene="hypD"
                     /locus_tag="OO372_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6340092.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8038..8310)
                     /gene="hypC"
                     /locus_tag="OO372_000493"
     CDS             complement(8038..8310)
                     /gene="hypC"
                     /locus_tag="OO372_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6340093.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8301..9173)
                     /gene="hypB"
                     /locus_tag="OO372_000494"
     CDS             complement(8301..9173)
                     /gene="hypB"
                     /locus_tag="OO372_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6340094.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9177..9527)
                     /gene="hypA"
                     /locus_tag="OO372_000495"
     CDS             complement(9177..9527)
                     /gene="hypA"
                     /locus_tag="OO372_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6340095.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9739..10200
                     /gene="hycA"
                     /locus_tag="OO372_000496"
     CDS             9739..10200
                     /gene="hycA"
                     /locus_tag="OO372_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6340096.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10325..10936
                     /gene="hycB"
                     /locus_tag="OO372_000497"
     CDS             10325..10936
                     /gene="hycB"
                     /locus_tag="OO372_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6340097.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10933..12759
                     /gene="hycC"
                     /locus_tag="OO372_000498"
     CDS             10933..12759
                     /gene="hycC"
                     /locus_tag="OO372_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6340098.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12762..13685
                     /gene="hycD"
                     /locus_tag="OO372_000499"
     CDS             12762..13685
                     /gene="hycD"
                     /locus_tag="OO372_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6340099.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13703..15400
                     /gene="hycE"
                     /locus_tag="OO372_000500"
     CDS             13703..15400
                     /gene="hycE"
                     /locus_tag="OO372_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6340100.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15410..15952
                     /gene="hycF"
                     /locus_tag="OO372_000501"
     CDS             15410..15952
                     /gene="hycF"
                     /locus_tag="OO372_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR6340101.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15952..16719
                     /gene="hycG"
                     /locus_tag="OO372_000502"
     CDS             15952..16719
                     /gene="hycG"
                     /locus_tag="OO372_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR6340102.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16716..17126
                     /gene="hycH"
                     /locus_tag="OO372_000503"
     CDS             16716..17126
                     /gene="hycH"
                     /locus_tag="OO372_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR6340103.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17119..17589
                     /gene="hycI"
                     /locus_tag="OO372_000504"
     CDS             17119..17589
                     /gene="hycI"
                     /locus_tag="OO372_000504"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR6340104.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17614..18387
                     /locus_tag="OO372_000505"
     CDS             17614..18387
                     /locus_tag="OO372_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340105.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18549..18860
                     /locus_tag="OO372_000506"
     CDS             18549..18860
                     /locus_tag="OO372_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCR6340106.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18857..19276
                     /locus_tag="OO372_000507"
     CDS             18857..19276
                     /locus_tag="OO372_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6340107.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19390..20814)
                     /gene="ascB"
                     /locus_tag="OO372_000508"
     CDS             complement(19390..20814)
                     /gene="ascB"
                     /locus_tag="OO372_000508"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR6340108.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20823..22280)
                     /gene="ascF"
                     /locus_tag="OO372_000509"
     CDS             complement(20823..22280)
                     /gene="ascF"
                     /locus_tag="OO372_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR6340109.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22540..23550
                     /gene="ascG"
                     /locus_tag="OO372_000510"
     CDS             22540..23550
                     /gene="ascG"
                     /locus_tag="OO372_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6340110.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIT
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23698..24225
                     /gene="hydN"
                     /locus_tag="OO372_000511"
     CDS             23698..24225
                     /gene="hydN"
                     /locus_tag="OO372_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6340111.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24378..26630
                     /gene="hypF"
                     /locus_tag="OO372_000512"
     CDS             24378..26630
                     /gene="hypF"
                     /locus_tag="OO372_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6340112.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26758..27891)
                     /gene="norW"
                     /locus_tag="OO372_000513"
     CDS             complement(26758..27891)
                     /gene="norW"
                     /locus_tag="OO372_000513"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6340113.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27888..29327)
                     /gene="norV"
                     /locus_tag="OO372_000514"
                     /pseudo
     CDS             complement(27888..29327)
                     /gene="norV"
                     /locus_tag="OO372_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
     gene            29514..31028
                     /gene="norR"
                     /locus_tag="OO372_000515"
     CDS             29514..31028
                     /gene="norR"
                     /locus_tag="OO372_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6340114.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31025..31990)
                     /gene="gutQ"
                     /locus_tag="OO372_000516"
     CDS             complement(31025..31990)
                     /gene="gutQ"
                     /locus_tag="OO372_000516"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6340115.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31983..32756)
                     /gene="srlR"
                     /locus_tag="OO372_000517"
                     /gene_synonym="gutR"
     CDS             complement(31983..32756)
                     /gene="srlR"
                     /locus_tag="OO372_000517"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6340116.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32823..33182)
                     /gene="gutM"
                     /locus_tag="OO372_000518"
     CDS             complement(32823..33182)
                     /gene="gutM"
                     /locus_tag="OO372_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6340117.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33288..34056)
                     /gene="srlD"
                     /locus_tag="OO372_000519"
                     /pseudo
     CDS             complement(33288..34056)
                     /gene="srlD"
                     /locus_tag="OO372_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34060..34431)
                     /gene="srlB"
                     /locus_tag="OO372_000520"
                     /gene_synonym="gutB"
     CDS             complement(34060..34431)
                     /gene="srlB"
                     /locus_tag="OO372_000520"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6340118.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34442..35401)
                     /gene="srlE"
                     /locus_tag="OO372_000521"
                     /gene_synonym="gutE"
     CDS             complement(34442..35401)
                     /gene="srlE"
                     /locus_tag="OO372_000521"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6340119.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35398..35961)
                     /gene="srlA"
                     /locus_tag="OO372_000522"
                     /gene_synonym="gutA"
     CDS             complement(35398..35961)
                     /gene="srlA"
                     /locus_tag="OO372_000522"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR6340120.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36217..37302
                     /gene="mltB"
                     /locus_tag="OO372_000523"
     CDS             36217..37302
                     /gene="mltB"
                     /locus_tag="OO372_000523"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6340121.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37447..37944
                     /gene="pncC"
                     /locus_tag="OO372_000524"
     CDS             37447..37944
                     /gene="pncC"
                     /locus_tag="OO372_000524"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6340122.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38024..39085
                     /gene="recA"
                     /locus_tag="OO372_000525"
     CDS             38024..39085
                     /gene="recA"
                     /locus_tag="OO372_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6340123.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39313..39813
                     /gene="recX"
                     /locus_tag="OO372_000526"
     CDS             39313..39813
                     /gene="recX"
                     /locus_tag="OO372_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6340124.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39941..42571
                     /gene="alaS"
                     /locus_tag="OO372_000527"
     CDS             39941..42571
                     /gene="alaS"
                     /locus_tag="OO372_000527"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6340125.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42806..42991
                     /gene="csrA"
                     /locus_tag="OO372_000528"
     CDS             42806..42991
                     /gene="csrA"
                     /locus_tag="OO372_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6340126.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43307..43399
                     /locus_tag="OO372_000529"
     tRNA            43307..43399
                     /locus_tag="OO372_000529"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43341..43343,aa:Ser,seq:gct)
     gene            43403..43479
                     /locus_tag="OO372_000530"
     tRNA            43403..43479
                     /locus_tag="OO372_000530"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43437..43439,aa:Arg,seq:acg)
     gene            43543..43619
                     /locus_tag="OO372_000531"
     tRNA            43543..43619
                     /locus_tag="OO372_000531"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43577..43579,aa:Arg,seq:acg)
     gene            43683..43759
                     /locus_tag="OO372_000532"
     tRNA            43683..43759
                     /locus_tag="OO372_000532"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43717..43719,aa:Arg,seq:acg)
     gene            43958..44034
                     /locus_tag="OO372_000533"
     tRNA            43958..44034
                     /locus_tag="OO372_000533"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43992..43994,aa:Arg,seq:acg)
     gene            44315..44881
                     /gene="yqaB"
                     /locus_tag="OO372_000534"
     CDS             44315..44881
                     /gene="yqaB"
                     /locus_tag="OO372_000534"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6340127.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44878..45306
                     /locus_tag="OO372_000535"
     CDS             44878..45306
                     /locus_tag="OO372_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6340128.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45379..46935
                     /gene="gshA"
                     /locus_tag="OO372_000536"
     CDS             45379..46935
                     /gene="gshA"
                     /locus_tag="OO372_000536"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6340129.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            47085..47600
                     /gene="luxS"
                     /locus_tag="OO372_000537"
     CDS             47085..47600
                     /gene="luxS"
                     /locus_tag="OO372_000537"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6340130.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47664..49202)
                     /gene="emrB"
                     /locus_tag="OO372_000538"
     CDS             complement(47664..49202)
                     /gene="emrB"
                     /locus_tag="OO372_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6340131.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49219..50391)
                     /gene="emrA"
                     /locus_tag="OO372_000539"
     CDS             complement(49219..50391)
                     /gene="emrA"
                     /locus_tag="OO372_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR6340132.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50518..51048)
                     /gene="emrR"
                     /locus_tag="OO372_000540"
     CDS             complement(50518..51048)
                     /gene="emrR"
                     /locus_tag="OO372_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6340133.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(51139..51474)
                     /gene="ygaH"
                     /locus_tag="OO372_000541"
     CDS             complement(51139..51474)
                     /gene="ygaH"
                     /locus_tag="OO372_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6340134.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51464..52201)
                     /gene="ygaZ"
                     /locus_tag="OO372_000542"
     CDS             complement(51464..52201)
                     /gene="ygaZ"
                     /locus_tag="OO372_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6340135.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52325..53509)
                     /locus_tag="OO372_000543"
     CDS             complement(52325..53509)
                     /locus_tag="OO372_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6340136.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53800..54792)
                     /gene="proX"
                     /locus_tag="OO372_000544"
     CDS             complement(53800..54792)
                     /gene="proX"
                     /locus_tag="OO372_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6340137.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54850..55914)
                     /gene="proW"
                     /locus_tag="OO372_000545"
     CDS             complement(54850..55914)
                     /gene="proW"
                     /locus_tag="OO372_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6340138.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55907..57109)
                     /gene="proV"
                     /locus_tag="OO372_000546"
     CDS             complement(55907..57109)
                     /gene="proV"
                     /locus_tag="OO372_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6340139.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57465..58424)
                     /gene="nrdF"
                     /locus_tag="OO372_000547"
     CDS             complement(57465..58424)
                     /gene="nrdF"
                     /locus_tag="OO372_000547"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6340140.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58434..60578)
                     /gene="nrdE"
                     /locus_tag="OO372_000548"
     CDS             complement(58434..60578)
                     /gene="nrdE"
                     /locus_tag="OO372_000548"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6340141.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60551..60961)
                     /gene="nrdI"
                     /locus_tag="OO372_000549"
                     /pseudo
     CDS             complement(60551..60961)
                     /gene="nrdI"
                     /locus_tag="OO372_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60958..61203)
                     /gene="nrdH"
                     /locus_tag="OO372_000550"
     CDS             complement(60958..61203)
                     /gene="nrdH"
                     /locus_tag="OO372_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6340142.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61451..61780)
                     /locus_tag="OO372_000551"
     CDS             complement(61451..61780)
                     /locus_tag="OO372_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6340143.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61932..62276
                     /locus_tag="OO372_000552"
     CDS             61932..62276
                     /locus_tag="OO372_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6340144.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62313..62762)
                     /gene="alaE"
                     /locus_tag="OO372_000553"
     CDS             complement(62313..62762)
                     /gene="alaE"
                     /locus_tag="OO372_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6340145.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14885 a        16303 c        17038 g        15005 t
ORIGIN      
        1 cgatggcata ttttatgttg tcattaatga tttcaataat atgtggaagg tgatggaatt
       61 tcagcaacag atctattgcc tcttgttgct ctgaatcatt taaatcgaaa aaccagtcac
      121 caccgctggc aagccacata tttccatcgc cagtttcgaa tgcatccaac gccatcgctt
      181 cgtggttgcc tttaaccgac gtaaaccagg gttggtttag caggcgcagc acgttaagac
      241 tcttcggccc acgatcaata ttatcgcctg tggaaataag taagtcggtt tcagggtaaa
      301 aagagagttg atgttaacgg gattgtaata actgatattc accatgaata tcaccaacga
      361 cccatatatg gcgatagtga tgggcattga ttttttgata gcgtgtagat ggcatggttt
      421 taccctgtaa aataagcttt cctattatac agggtatttt tatttgattc gtcagttgtc
      481 gttaatattc ccgatagcaa aagactatcg ggaattgtta ttacaccaga ctcttcaagc
      541 gataaatcca ctccagcgcc tgacgcggag tcagtgaatc cgggtcgaga ttttccagtg
      601 cttcgaccgc aggcgaagtt tcttctggca ctgacagcaa agacatctgc gtaccatcca
      661 cttgcgtcgc ggcggcgttc ggcgaaatgc tttccagctc acgcagtttt tgccgtgcgc
      721 gcttaataac ctctttcggt acgcccgcca gagcagcaac cgccagaccg tagcttttgc
      781 tcgccgcgcc gtcctgcacg ctgtgcataa aggcgatagt gtcgccgtgc tccagcgcat
      841 cgagatgtac gttggcgacg ccttccattt tctccggtag ctgggtcagc tcgaaatagt
      901 gggtggcaaa cagcgtcaat gccttaatct tattcgccag attttctgcg cacgcccacg
      961 ccagcgacag accatcgtag gtggacgttc cacgcccgat ctcatccatt aacaccagac
     1021 tgtattcggt ggcgttatgt aaaatattgg cggtttcagt catctccacc ataaaggttg
     1081 agcgcccgga agccaggtca tctgccgcgc ctacgcgggt aaagatgcga tcgataggtc
     1141 caatctcgac tttttgtgcc ggtacatagc tgccgatgta ggccatcagc gcaatcagtg
     1201 cggtctggcg catataggta cttttaccgc ccatgttcgg accggtgatg atcaacatac
     1261 ggcgctgcgg cgacagattc agcgggttag cgataaatgg ctcattcagt acttgttcaa
     1321 ctaccggatg gcgaccttcg gtaatgcgaa tgcccggttt atcaatgaag gtcgggcagg
     1381 tgtagttcag ggtataggcc cgttccgcca ggttcaccag cacgtcgagt tccgccagcg
     1441 cgctcgcgct ctgttgcaac gcttccagat gcggcaacag caggtcgaac aactcttcat
     1501 aaagctgttt ttccagtgcc agtgctttgc cttttgaggt gagaacttta tcttcgtact
     1561 cttttagctc tggaatgatg tagcgctcgg cgtttttcag cgtctggcga cgcatgtagt
     1621 tgatgggtgc cagatggctt tgcccacggc tgatttgaat gtagtagccg tgcaccgcat
     1681 taaagccaac tttcagcgtg tccaggccgg tacgttcacg ctcgcggact tccagacgct
     1741 ccagataatc ggtcgcgccg tcagccagcg cgcgccactc atccagctct tcgttatagc
     1801 ccgatgcgat aacaccaccg tcgcgtacca gcaccggcgg tgtgtcgatg attgctcgct
     1861 ccagcagatc gcgcagctcg gcaaactcgc ccatcttctc acgtagcgcc tgtaccggtg
     1921 cactatcgac agtttctaac tgcgcacgca gctccggcag ttgctggaaa gcgtggcgca
     1981 tacgggccag atcgcgtggg cgagcagttc gtaaagccag acgtgccaga atacgttcca
     2041 ggtcgccgac ctgacgcagt accggctgca actcggcggt gaaatcctgc aatgcgccaa
     2101 tagtttgctg gcgctcaagc aacacgcggg tatcgcgcac tggcatatgc agccagcgtt
     2161 tcagcatacg actacccatc ggcgtgacgg tgcagtcgag cacagaagcc agcgtatttt
     2221 ccgcaccgcc cgccaggttc tgagtaattt ccaggttacg acgtgtcgcg gcatccataa
     2281 tgatgctgtc ctgctcacgt tccatggtga tagaacgaat atgcggcagg gtcgtgcgtt
     2341 gggtatcttt cgcatactgc aacagacaac cggcagcaca aagtccgcgc ggcgcgttct
     2401 cgacgccaaa accgaccaga tcgcgggtgc caaattgcag attcaactgc tggcgcgcgg
     2461 tgtcgatttc aaactcccac agcgggcgac ggcgcaggcc gcgacggcct tcaatcagcg
     2521 acatctcggc gaaatcttct gcatacagca gttccgccgg attagtgcgt tgcagctctg
     2581 ccgccatcgt ttcgcggtcg gccggttcgc tcaggcgaaa acgcccggag ctgatatcca
     2641 gcgtcgcgta gccgaaacct ttgctgtcct gccagatagc cgccagcagg ttgtcctgac
     2701 gctcctgtaa cagggcttca tcgctgatgg tgcctggcgt aacgatacgc acaactttgc
     2761 gctcaaccgg acctttgctg gtcgccggat cgccaatttg ttcgcagatg gcaacggact
     2821 ctccctgatt caccagtttg gcgagatagt tttccaccgc atggtaggga atccccgcca
     2881 tcgggatcgg ctctcccgcc gaagcaccgc gtttggtcag tgaaatatcc agcagttgcg
     2941 acgcgcgttt tgcgtcgtca taaaacagtt cataaaaatc acccatccgg taaaacagca
     3001 ggatctcggg atgctgggct ttcagcctga gatactgctg catcatgggc gtatgggcgt
     3061 cgaaattttc tattgcactc atggggttat gtccggttcc ctgatattaa atggggtgtg
     3121 atggttttta ttttcgttgt aatcacataa ggcgcacatg ataccggagt taatcatttc
     3181 aggggaagaa gcgaacggcg agttcttgat gctggttccg ggaaagatgc tgttttgcag
     3241 cttttgcgtg atgcttatga agcaagtacg caaaattgcg tcggttgaat aaacctcctc
     3301 cttcagagcc atagtcccct ttcaaattaa gttgtaatat aaatacacct taagagtatg
     3361 gaggcagcat gtccggcaag cgtatctctc gtgaaaaact gacgattaaa aaaatgatcg
     3421 atctttatca agcgaaatgc ccgcaggcgt cagcggagcc ggagcattac gaggcgttgt
     3481 ttgtttacgc gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa ccagcctgta
     3541 agcagtgtcc ggtgcactgt taccagcctg caaagcgtga agagatgaaa cagattatgc
     3601 gttgggccgg gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt catctcatcg
     3661 atgacaagcg tccggtacca gaactgccgg aaaaatatcg tccgaaaaaa cctcgtgaat
     3721 agtatgccgg acaggcagat ctgtccggca atttgcagtt aaatcaatgc cgatttatca
     3781 attcccagcc gcttcatccg tgacagcagg gtcgtgcgtt tcagccccag acgttgcgca
     3841 gcgcctttag gcccggcaac cacgccgttg gtttctttca gcacgcgcac aatcaactga
     3901 tattcatctt cgccctcctg ggcgacaacc gttgcggcag gcggcgtttc aggttccggt
     3961 aaaacaatat ctggcaatga cagctgcagc acgttaccgc gtgttagcaa taccgcgcgc
     4021 tcaatgacgt tttccagttc gcgtacgtta cccggccact ccatgttgct caaggtgcgc
     4081 agcgtctcgg caggaatgct gtcgatattg cgccccagac gacgggcaat tttgaaggta
     4141 aaggctttcg ccagcagcgg aatatcttcc ggacgctcgc gtagtggcgg caggtgaatc
     4201 gggaatacgt tcaggcggta atagagatcg ctacggaact ctcggtcggc gaccattttt
     4261 ttcagatcgc ggttagtcgc ggcaattaaa cgtacgtccg tctgaatgat tttgttgctg
     4321 ccgagacgtt caaactcctg ttcctgcaat acacgcagca acttcggctg taactccagt
     4381 ggcatatcgc ccacttcgtc gaggaacaga gagcttttat ccgccagttc aaaacgacca
     4441 atgcgctggg cgctggcacc ggtaaaagcc ccacgctcat gaccaaacag atcgctttcc
     4501 agcaatccgg caggcatcgc cgcgcagttc attttgacca tgcggcgatt attacgccca
     4561 ctgagattat ggatcgcgcg ggcaatcagc tctttacccg tgccagtttc accgaggatc
     4621 agcacggtac tgtcactttg cgccaccatt tcaacttgtt taagcacgct gtacatggct
     4681 tcgctgcggc caataatctc gccaaattca ctatcaacat tgttgagctg ctcggtcagg
     4741 gcgaggtttt catcaaccag ccgttctttc agacgatgga tttcctgata ggcgagggcg
     4801 ttatcgacag cgattgccac acgttcggca atctggcgca gcaaattcag attggtagtg
     4861 gtaaacactt tctcttcgca ttgcgccagt ttcagcacgc ccagcatggt gtcgccagac
     4921 atcagcggta acaggcacaa ggtttgaatc tggttgccct aggtgtcgaa caacatgcgt
     4981 tcataggggg ctaaatcgtc ccgctcgtgg agattgatca gcagcatctc tttacttttg
     5041 aacacgcgtt cggtgagggt tccggcttca tcgacttcgc tctgttcgtg ggcgggatgt
     5101 tgtttatcaa gatagtgagt ggagtagatg ttgagtttgt ttttacggtg gctgcgtaag
     5161 acgatactga tatcgtcaat gtcgaaatag taatggattt ctttggcgac ttcgctgacc
     5221 agttcgtcca tatccaggcg ggaaagcacc gcgttggtaa tggcaaccag gatgcggaag
     5281 ttatcgcgct cccggcataa caactcatag tcgacattgt tgacgacgcg gctctggatt
     5341 tgttcggtga cgacagaaac gatctgcgta aatgtttgca gacgattgaa ctctttttcg
     5401 ctccagggac gatcgtcata acgaataaat tcacagccac caaaaatatg cccttccgcc
     5461 gccagtggca tcaggcaata gtgaccaaat ttggggtata gcccacctgc gaccagctgc
     5521 ggccaggttt cacaaaattc ttcgtaactg caatgcagcg tatcagggcg cgacaaaatg
     5581 cggcgtaccg gaccgtgtgc cagaacagtt tcgtcttcat atttaatggg gtgtcttttt
     5641 cacgcgatgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca atagccgcgt
     5701 tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc gaggccagat
     5761 cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg agatcactca
     5821 tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc aaaaggtcat
     5881 tgactaacta taggagattt agaattttat tagcatatac gcggaagagg ttcggcgtgt
     5941 ggtaaatcga gggttcgttt cacaccatac agaccggcaa gacgaacacc tttacgttcc
     6001 accacttcac caattagcgc cgcgtctttc cccagtggat gggaatgtaa cgctgccagc
     6061 acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt tgccttcgtt ggcaaagtta
     6121 agggcgtcca gtcccagcag ttcgcaaacg ccgcgcacgg caggtttaac cggcagtgcc
     6181 acttctgaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac taccgcgttc
     6241 acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg cagcgtctga
     6301 ataagcggcg tcagtaccgc gcagtcgctg accagttcgc catccagccc cagctgctca
     6361 cgcaggttaa ggatagtcgc cccgtggtcc ccgagcgtgc cgctaaccag taatacatcg
     6421 cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc catgccagcg
     6481 gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc gccagtaacg
     6541 atggcaatgc ctgccgcgcg cgcggtttct gccatgctgg tcactacggc tttcagtgtc
     6601 tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg aatagcgcca
     6661 ctgaccgcaa cgtcattggc tgtgccgcaa atcgccagct tgccgatatt accgccagga
     6721 aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc gccttctgct
     6781 accagctgcg ccagatcaag acgtgcctgg tcttcctgct ctgccagcca cgggttggca
     6841 aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc gctgccgtgg
     6901 gcgagttgga tattattcac gcttcattct cctgctgacg atactgatac cacgcggcgc
     6961 acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta caggtgttac
     7021 caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca caacgcgcgc
     7081 gcgggtcatc gcagacctgc tgcggtgctg ggcggaaatg tgcttcggca tcgaatcgtt
     7141 gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg cgccattcgc
     7201 tgtcgccgtt gacacagaac acatcagcaa ttgcctgttg cgccagcagg ttaccggcat
     7261 ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt ttctgctcca
     7321 ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc accagcggac
     7381 gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata ctaacgtggc
     7441 ccggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg cgcagcgtcg
     7501 ggataagcgt aatatgctgg cagaagaagt aaaaattctg cacatcgcgg gctttcgcct
     7561 gttgcagggt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg aagaatacga
     7621 ctttacgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag taaacgatgc
     7681 gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttcccc ggcacgcgca
     7741 tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc acgcaggtgt
     7801 cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata aactcaacgt
     7861 tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga ccaccgcaca
     7921 cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca cgttcgcgca
     7981 gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca aaacgcatta
     8041 tttttcctcg ccatacaaca gcgcgccgac atccggctca acgtcaaaca tgttttgtaa
     8101 ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa agccaacgtg
     8161 taccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc cgactaacgt
     8221 taaatcgaca tcgcgctgaa tgccgcagac gtcgactttt gcctggttgc cgtcaatggt
     8281 gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt cagccactgg
     8341 tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg attgacttcg
     8401 cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag gtcaactttg
     8461 ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg tttgtcttca
     8521 ccttcggtaa cggaaagcac cgccactttg tgtttttcac cgagatcgaa gctggccggg
     8581 catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag tggcagacgc
     8641 ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt gttcacctga
     8701 atcgctggtg tgccggtagc gcgaatgcgt gcggcatcgt tcacggtttg ctggtcacct
     8761 tcaataaccg cgcacggaac gctgtctttc aggcgcatta gggtttccgt cagcagggtg
     8821 gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg ggcagcaaag
     8881 cgcgcgcggt tgcgttcagc cagacggtta tttttgtcca gcacgtcaat ttcgacttcc
     8941 agcatccgac gctggctcat gcccggcgcg tgagtgcccg cttcgccatg accgtaatgc
     9001 aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc ggtgattttc
     9061 atcttcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg agggttatgt
     9121 tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca catcgctcac
     9181 tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat ctgtagcatg
     9241 tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac atactgttgg
     9301 catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca accttccgcc
     9361 acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc gacacaagaa
     9421 aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt tgcggcctgc
     9481 tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc gcctcccatt
     9541 aactattgcc agctacaagc aataattgtg ccagtgttga ttatccctgc ggtgaataat
     9601 gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta gcttaaaaat
     9661 ctatttaata acaataaatt aaaagttggc atagaaaatg cttaaagctg gcatctctgt
     9721 taaacgggta acctgacaat gactatttgg gaaataagcg agaaagccga ttacatcgca
     9781 cagcggcatt gtcgcctaca ggaccagtgg cacatctact gcaattcgct ggttcagggg
     9841 atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga caaagaactc
     9901 tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg ctttaacagc
     9961 cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg gattgcgcag
    10021 gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga tcgcgaacag
    10081 gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata caccgctatt
    10141 gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc ggcagcatga
    10201 gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg tgtcgatgag
    10261 tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac ctgaggaatg
    10321 cctggtgaat cgttttgtaa ttgttgactc cacgctctgt atcggctgcc acacttgtga
    10381 ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc gcctgagagt
    10441 gatgctgaat gaaaaagaat ctgcgccgca gctctgtcac cactgtgaag atgcaccctg
    10501 tgcggtggtt tgcccggtta acgccatcac ccgcattgat ggggccgtgc agttgaatga
    10561 aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg caattgaatt
    10621 ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag cgccaccggc
    10681 accgcctgct ccggcgcgta tcagtacgct gcttgactgg gtgccgggta ttcgcgcgat
    10741 cgccgtcaaa tgtgaccttt gcagttttga tgaacaaggt ccggcctgcg tgcggatgtg
    10801 cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca gcaaacgtaa
    10861 gcgtgagctg acctttaaca cggactttgg cgatctcacc ttgtttcagc aggctcaaag
    10921 tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg gtttgtcgcc
    10981 gccgctgttc tggcatttct cttttctttt caaaaagcgt taagtggctg gatagctgga
    11041 attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt actgactggc
    11101 gcggttggcg tgagcggtgc gctgtcgctg gtaagctacg atgtgcaaat ctctccgctt
    11161 aacgcgattt ggctgattac gctcggtctg tgcggtctgt ttgtcagcct ctacaacatt
    11221 gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat gttgatggct
    11281 gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat ggccgaagtc
    11341 atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact gtggtttgcg
    11401 ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg gcagcgttat
    11461 ggcacgctgg atctgtgcct actggatatg cgtatgcaac agctgccgct cggttccgat
    11521 atctggctgc tcggtgtgat tggctttggc ctgctggccg ggattattcc gctgcacggc
    11581 tgggtgccgc aggcacatgc taacgcctct gcgccagctg ccgcgttgtt ttctacggta
    11641 gtcatgaaaa ttggcctgct gggcatgtta accctgtcac tgctgggcgg taatgcaccg
    11701 ctgtggtggg ggatcgcgct gctggtgctc ggcatgatca ccgcgttcgt cggtggtctg
    11761 tatgcgttga tggaacacaa catccagcgc ctgctggctt accacaccct ggaaaatatc
    11821 ggcattatcc tgctggggct gggcgctggc gtaacgggta tcgcgctcga acaaccggcg
    11881 ctgattgctc ttggcctggt cggtggtctg taccatctgc ttaaccatag cctgttcaaa
    11941 agcgtactgt tcctcggggc ggggagcgtc tggttccgta ccggtcatcg cgatatcgaa
    12001 aaactcggtg gtattggcaa gaaaatgccg gttatctcca tcgccatgtt agtcgggttg
    12061 atggcaatgg ctgcgctgcc gccactgaac gggtttgccg gggaatgggt tatctatcaa
    12121 tccttcttca aactgagcaa tagcggcgcg tttgttgccc gtctgctggg gccgctgctc
    12181 gctgtgggtc tggcaattac cggtgcgctg gcggtgatgt gtatggcgaa agtctatggc
    12241 gtcacgttcc tcggcgcgcc gcgtaccaaa gaagccgaaa acgccacctg tgcgccgctc
    12301 ctgatgagcg taagcgtagt ggcactggcg atttgctgcg taattggcgg tgttgctgcg
    12361 ccgtggctgc tgccgatgct ctctgctgct gtacctctgc ctctggagcc tgctaacacc
    12421 accgtttctc aaccaatgat cacattgctg ctgattgcct gcccgctgct gccattcatc
    12481 attatggcga tttgcaaagg cgaccgcctg ccgtcacgtt cccgcggtgc ggcctgggtg
    12541 tgcggctacg accatgaaaa atcaatggtg attaccgctc acggttttgc catgccggtt
    12601 aaacaggcgt ttgcgccagt gctgaaactg cgcaaatggc tgaatccggt gtcgctggtg
    12661 ccgggctggc agtgcgaagg tagtgcgttg ctgttccgcc ggatggcgct ggttgaactg
    12721 gcggtactgg tggtgattat tgtttcacga ggagcctgag aatgagtgtt ttatatccgt
    12781 taattcaggc gctggtgtta tttgccgttg cgccgctgct ctccggtatt acccgcgtgg
    12841 cgcgcgcccg tctgcataac cgtcgtgggc cgggtgtgtt gcaggagtat cgtgacattt
    12901 tcaaactgct gggccgccag agcgttggcc cggatgcctc cggctgggtg ttccgcctga
    12961 cgccgtatgt gatggtgggc gtcatgctga ctatcgctac tgcgctgccg gtggtgaccg
    13021 tcggttctcc gctgccgcaa ctgggtgatt taatcacctt actgtatctc tttgccatcg
    13081 cgcgtttctt ctttgccatt tctggtctgg ataccggtag cccgtttacc gctatcggcg
    13141 cgagccgtga agcgatgctt ggcgtgctgg tcgaaccgat gctgctgctt ggtctgtggg
    13201 ttgccgcaca ggttgccggt tccaccaaca tcagcaacat caccgacacc gtttatcact
    13261 ggccgctgag ccagagcatc ccgctggtac tggcgctttg tgcctgtgcg ttcgccacct
    13321 ttatcgaaat gggcaaactg ccgttcgacc tggcggaagc cgagcaggag ctgcaggaag
    13381 gcccgctctc tgaatacagc ggcagcggct ttggcgtcat gaaatggggt atcagcctga
    13441 aacagctggt ggtgctgcag atgttcgtcg gcgtatttat cccgtgggga cagatggaaa
    13501 ccttcaccgc cggtgggctg ctgctggcgc tggtgattgc catcgtcaaa ctggtcgtcg
    13561 gcgtcctggt tatcgcgctg ttcgaaaaca gcatggcccg tctgcgtctt gatattactc
    13621 cgcgcattac ctggactggg tttggctttg catttttagc gttcgtctcc ttgctggcgg
    13681 cgtgattaaa gagagtttga gcatgtctga agaaaaatta ggtcaacatt atctcgccgc
    13741 gctgaatgag gcatttccgg gcgtcgtgct ggaccacgcc tggcagacca aagatcagct
    13801 gactgtcacc gtaaaggtga actatctgcc ggaagtggtg gagtttctct actacaagca
    13861 ggggggctgg ctgtcggtgc tttttggtaa cgacgaacgc aaactgaatg gtcattacgc
    13921 cgtttattac gtgctgtcga tggagaaggg gactaagtgc tgggtaacgg ttcgcgtcga
    13981 agttgatgcc aacaaaccgg agtatccgtc cgtgacgccg cgcgttccgg cggcggtgtg
    14041 gggcgagcgc gaagtgcgcg atatgtacgg tttgattccg gttggtctgc cggatgaacg
    14101 ccgtctggtg ctgccggatg aactttatcc gctgcgtaaa gacagcatgg attatcgtca
    14161 gcgtccggca ccgaccaccg atgctgaaac ctacgagttc atcaacgaac tgggcgacaa
    14221 gaaaaacaac gtcgtgccga ttggtccgct gcacgtcact tctgacgaac cgggccactt
    14281 ccgtctgttc gtcgatggcg aaaacattat cgacgccgac taccgtctgt tctacgttca
    14341 tcgcggcatg gaaaaactgg cggaaacccg tatgggttac aacgaagtga ccttcctctc
    14401 tgaccgtgtg tgcgggatct gcggctttgc ccacagcacc gcctacacca cgtcggtaga
    14461 aaacgcgatg ggtattcagg tgccagaacg tgcgcagatg atccgcgcca ttctgctgga
    14521 ggtggaacgc ctgcactcgc atctgctcaa cctcggtctc gcctgccact ttaccggctt
    14581 tgactccggc tttatgcagt tcttccgcgt gcgtgaaacc tccatgaaaa tggcagagat
    14641 cctcaccggt gcgcgtaaaa cctacggcct gaacctgatc ggcgggattc gtcgcgatct
    14701 gttgaaagat gacatgatcc agacccgcca gctggcgcaa cagatgcgtc gtgaagtgca
    14761 ggagctggtg gatgtgctgc tgagtacgcc gaacatggaa cagcgcaccg tcggcattgg
    14821 tcgtctggac ccggaaatgg cccgcgattt cagtaacgtt ggcccgatgg ttcgcgccag
    14881 cggtcacgcc cgcgatactc gcgccgatca cccgtttgtc ggttatggcc tgctgccaat
    14941 ggaagtccac agcgagcagg gctgcgacgt tatttcccgt ctgaaagtgc gtatcaacga
    15001 agtttatacc gcgctgaaca tgatcgacta cggtctggat aacctgccgg gcggtccgct
    15061 gatggtggaa ggctttacct acattccgca ccgtttcgcg ctgggctttg ccgaagcgcc
    15121 gcgcggcgac gatatccact ggagcatgac cggcgacaac cagaagctgt accgctggcg
    15181 ctgccgtgcc gcgacctacg cgaactggcc gaccctgcgc tacatgctgc gcggtaacac
    15241 cgtttccgat gcgccgctga ttatcggtag cttggaccct tgctactcct gtaccgaccg
    15301 catgaccgtg gtcgatgtgc gtaagaaaaa gagcaaagtg gtgccgtaca aagaactcga
    15361 gcgttacagc attgagcgta aaaactcgcc gctgaaataa ggaatcgcca tgtttacctt
    15421 tatcaaaaaa gtcatcaaaa ccggcacggc gacctcgtct tatccgctgg agccgattgc
    15481 ggttgataaa aacttccgtg gtaagccgga gcagaacccg cagcagtgca tcggctgtgc
    15541 ggcctgcgtc aatgcctgcc cgtcaaacgc cttaacggtt gaaactgacc tcgccaccgg
    15601 tgagctggcg tgggagttta atctcgggcg ctgcatcttc tgtggacgct gcgaagaagt
    15661 ttgcccgacg gcggcgatca aactgtcgca agagtacgaa ctggcagtgt ggaagaaaga
    15721 agacttcctg caacagtccc gcttcgcgct gtgcaactgc cgcgtctgca atcgtccttt
    15781 cgccgtccag aaagagatcg actacgccat tgcgctgctt aagcacaacg gcgacagccg
    15841 cgcggaaaac caccgcgaaa gctttgagac ttgcccggaa tgtaagcgcc agaaatgcct
    15901 ggtgccgtcc gaccgtattg aactgactcg ccatatgaaa gaggccatct gatgagcaat
    15961 ttattaggcc cccgtgacgc caacggcatt ccggtcccga tgacggtgga tgaatccatc
    16021 gccagcatga aggcgtcgtt actgaaaaaa atcaaacgtt ctgcctacgt ttatcgcgtg
    16081 gactgcggcg gctgcaacgg ctgcgaaatc gaaattttcg ccacgctttc gccgctgttt
    16141 gatgcagaac gcttcggcat taaagtcgtt ccttcaccgc gtcatgcgga tattttactg
    16201 tttaccggcg cggtcacccg tgcaatgcga tcccctgcgc tgcgtgcgtg gcagtccgcg
    16261 ccggacccga aaatttgtat ctcctacggt gcctgcggta acagcggtgg tattttccac
    16321 gatctctact gcgtgtgggg cggtacggat aaaatcgtcc cggtagatgt ctacattcct
    16381 ggttgcccgc caacgcctgc cgccacgctg tacggctttg caatggcgct cggcctgctg
    16441 gagcagaaaa ttcacgcccg tggcccgggt gagcaggatg aacaacaggc ggaaatcctg
    16501 catggcgata tggtgcagcc gctgcgtgtg aaagtggatc gcgaagcacg ccgcctggcg
    16561 ggttatcgtt acggtcgtca gattgccgat gacttcctga cacagttagg gcagggcgaa
    16621 gaacaggttg cacgctggct ggaagcggaa aacgatccgc gtctgaacga gattgtcagc
    16681 catctgaatc atgttgttga agaggcgcgt atccgatgag tgaaaaggtg gtgttcagtc
    16741 aactgagccg taaatttatt gatgagaacg atgccacgcc cgccgaggcg cagcaggtgg
    16801 tctattacag cctggcgatt ggtcaccacc ttggggttat cgattgcctg gaagcggcgc
    16861 tcacctgccc gtgggatgaa tatctggcat ggattgccac tctggaggcg ggcagtgaag
    16921 cccgccgcaa aatggaaggc gtgccgaaat atggtgagat cgtcatcgac attaaccatg
    16981 tgccgatgct ggccaacgca ttcgataaag cccgggcagc gcaaacttcg cagcagaagg
    17041 aatggagtac aatgctgtta agtatgctgc atgatattca tcaggaaaac gccatctatt
    17101 tgatggtgag gagactgcgt gactgacgtt ttactctgtg ttggcaatag catgatgggc
    17161 gatgatggcg ctggcccgct gctggcggaa aagtgcgccg ccgcgccgaa aggtaactgg
    17221 gtggtgattg atggcggtag tgcaccggaa aacgacatcg tcgccatccg tgaactgcgc
    17281 cctacgcggt tactgattgt tgacgctact gatatggggc taaaccccgg cgaaatccgc
    17341 atcatcgacc cggatgatat cgccgagatg tttatgatga ctacccataa catgccgctg
    17401 aactatctta tcgaccagtt gaaagaagac attggcgaag tgattttcct cggcatccag
    17461 ccggatatcg tcggctttta ctacccgatg actcagccga ttaaagatgc ggtagaaacc
    17521 gtttatcaac gactggaagg ctgggaaggg aatggcggct tcgcgcagtt agcggtggaa
    17581 gaagaataat tctccatcag ggaattcagc gaaatgaata tcagaacagg gatgtgcgct
    17641 tttttattga gcttagtgct gcctgcacag gccaccagct ttaccgaata tctgccaatg
    17701 agtgacagcg aatacgcgca aaagcgggcg ctgaaacctc tcctgaccat gccttatgat
    17761 gccgagcaaa cctggcgttt tagaaaagtg ggcgttgctg gcgtgacgct tgaaaagatg
    17821 ccaaatgatg atagtgaatg gcaattgaac ggtaaagatc gcgccggtaa atcctggtct
    17881 gtgcctgtgg gtatgctggc aaacatagcg ggcaaagggc aactctatcg cgccgatctt
    17941 gatcgtaacg gcattcagga tctggttatc tggttaccaa gtaccggtaa tggtctggct
    18001 ccttatgcac atttaattct tatgacgttc acgcgcgaag gccgtccctg tgtctttgaa
    18061 cccaggggat tctacaccgc aagcaaaact ggcgtagatg atttgctgga tttgcaaggc
    18121 aatggtcaca cgcaattgct ggatatgcag tttgattccg gttactggat aaccagtctg
    18181 tatcaggtaa aagacgccaa atggcagcgt gttcacggct ggtttggcaa gttaagctat
    18241 cccgcgctca cgcgttttac ttataccccc aaccgtaaac tcgtcctcaa acccatcgcc
    18301 gggcgcgacc cgcaaacgga agatctggcg cagacgcagc gttgcttgat aaagggggat
    18361 gttcttgatg gggtgaacca gaactgactt tttgcagagt ggctatgagg attttggggg
    18421 taattatatc gcgctcaagt gtttacatat tgtgaacttg gatttgatga gctatagttt
    18481 ccgaacacat aaggatagaa ctcgcagatg tggctgttta cttactataa ggaagttaaa
    18541 acttaaaaat gcacatcatt tctaaagcgc catttgaaga gtctgcgcgt aaatatccta
    18601 acgacgctct ggctcttcag gcattgtatc gggttattaa ggaaacggat ttttctacgc
    18661 cagaagagat gcgaacggcg tttcccaacc tcgacaattt taaatatcgc aacaaatggt
    18721 acgttctgga tgtaggtggg aacaacctaa gggtgattgc ctacataaac tttgttaata
    18781 agcggttctt tgtgaagcac ataactaatc acgccgagta tgacaaactg acacgttact
    18841 acagagagaa caaagaatga ctgctaatgc tgcacgcgct gttaaagcaa caagagaact
    18901 ggtcaatgca gtcccctttc tgggaggtag tgactcagaa gatgattatc gtgaagcact
    18961 ggaactggtc gaatatttga ttgaagaaga tgacaccaat ccacttatcg actttctggc
    19021 gagccgcatt gcagaatatg aaaacaacaa ccaagagttt gcagagttcg acaaagccgt
    19081 tgcggcaatg cctgttggcg tggcgttact tcgcacgctg attgatcaac ataatctgac
    19141 ctatgcggat ttgaagaacg aaatcggctc taaatccctg gtcagtcaga ttttgtctgg
    19201 acaacgatcc ctgacgatat cccacattaa ggcactttca gcccgatttg gcgttaagcc
    19261 ggaatggttt ctttgatttt gactgatgcc aaaccccaga ttgcccgatg cgctgcgctt
    19321 atcgggcctt cctggttcgt gcgacatgta ggccggataa ggcgttcacg ccgcatccgg
    19381 cactgttacc tactctaaat cttccccatt actggcaatc acttttttat accaccagaa
    19441 cgatttctta cgtgtgcgcg tcagcgtgcc gttgcctgcg tcgtcacggt ctacgtagac
    19501 aaaaccgtag cgtttgctca tttcacccgt agaggcggag actaaatcaa tacagcccca
    19561 ggtggtgtag cccatcagcg gaatgccatc tgcaatcgct tcgcccattg cgcggatatg
    19621 ttcgcgtaaa tagctgatgc gatagtcatc gttaatctcg ccattggcag caagttcatc
    19681 ttttgcgccg aggccgtttt ccaccagaaa cagcggcttc tgataacggt cgtacatcat
    19741 gttcatggtg atacgcagac cgagtggatc aataccccag ccccagtcgc tcacttgcag
    19801 atacgggtta cgcagcgatt tcactacgtt cgccgcactg ctattgttgg cgttcatctc
    19861 cgccgaggcg cagcgcgagg cgtaatagct gaaagagaca aaatcgacgg tgtttttcag
    19921 gatttcatca tcgcccggtg ctttgtcgat ggttacccct ttttcgcgga atacgcgggc
    19981 agagtaagcc ggatacgtgc cccgcgcctg cacatcgata aaaaacaggt tttcccggtc
    20041 tttctccagc gccgcccaga catcttccgg cttgcaactg taagggtaga agttaccgcc
    20101 cgccagcata cagcccacct gattttgcgg gttaacctca tgggcgattt tggtggctag
    20161 cgcactggca accagctggt gatgcgcggc ctgatatttc acctgatcct gattttcacc
    20221 ttcttcaaac accagacccg cgccggagaa cgggctatgc aacataatgt tgatttcatt
    20281 gaaggtaagc cagtatttca ccagaccatc aaatgcttca aagcaggtcc gggcgtagcg
    20341 gctgaaaaac tccaccagct tgcggttacg ccaggagcca tattcggtga ccagatgcat
    20401 cggcacatcg aagtggcaca acgtgaccag cggttcgata ccgtactttt tacactcttc
    20461 aaagacagaa cgataaaaag caatgccctg ctgattgggc gtgatttcat cgccctgcgg
    20521 aaagagacgg ctccaggcaa ttgaggtacg gaaaaccttg aatcccatct ctgccatcag
    20581 ggcgatatct tctttataac gatgataaaa atccgtcgcc tcatggctgg gataaaactc
    20641 gtcatcgcgc aactgaaaac gtttttccag ccccagtttc accgccattc gatgctcgcc
    20701 gtgtgggatc atatcgacag tggtcagccc tttgccacct tcacggaacg caccttcaga
    20761 ctggttggcg gcaagcgcgc cgccccataa aaaaccttct ggaaatactg acattttcat
    20821 cctcaattaa gacttacttc tttggcgacg gtcggttgta cgctctgatg ctttcgcgcc
    20881 tcggcagccg cttcctcaac aggaatatcc tcaaagccga gcaacagtgt gaggataaac
    20941 gacagcacca ccgccagcgc catgaccgcg aacacccaga cgatgctcat tggattcgcc
    21001 ggatcgaaga attgcacgct ggtaaataac cctggcgctg ccattgagtg gctggcaagc
    21061 cccgccatac cggcaaccgc gccgcaaata aaaccgctga taagactggc gataagcgga
    21121 cgtttcaggc ggatcgccac gccgtataac gccggttcgg aaatccccgc cataatggct
    21181 gatgccgccg cagccagcgc cgtctggcgc agttccgggt ttttcgtttt ccacgccacc
    21241 gccagtgatg aaccgcccag cgacaggtta gcgccgatct ctgacggcat gaccatccct
    21301 tctttgccgg tttcggcaat ggtctgaatg atggttggcg taaagacgcg gtgcatcccg
    21361 gtcattacca gcagaggcca cagcgcgccc ataatggcga ctgaaagcca gcccagataa
    21421 ccatgaatgg tgtaaaccag cgccgaaatg gcgctaccga tccagatacc aatcgggcca
    21481 atcagcagga ttgccagcgg tgcggcaatc aacacaatca gcattggctt gaggaagttt
    21541 ttggtcaccg ccggtgtaat gcgatccacc cagcgttcga tatatgacag gcaccaggtc
    21601 atcaccagcg ccgggatcac tgtgtaggtg tatttcaccg cggtgaccgg aatcagggca
    21661 aattcgacat gttcaccctg ggccgctttc gccatcagtt caataaaact cggatgtacc
    21721 agcacacccg cgatggcaat cgccagcgac atattggttt taaatttgat ggcggcagat
    21781 gccgcgacca tcagcggcag gaagaagaaa gcaccgtcac caatcacatt cagaatggtt
    21841 aaggtcggcg atccttttgt cagcacgccg ctcatctcga ggatcatcgc cagcagtttg
    21901 accatcgatc cgccgataat cgccgggatc agcggtgaca tggtgccgat cagcgcatcg
    21961 aggatccccg caccaatgcg acgcagcgtg agtttgggtt tacccacggg ctgtgcgggc
    22021 tgcatatctc ccggcagcag gctgacgact tcctgaaagg cttgtgaaac ggtattgccg
    22081 ataatcacct gacactggtt gtcactacgt accacgccga gcacgccggg gatggttttt
    22141 aacgtcgggc tgtcgataag ctggtcatct ttgataacaa cgcgcaaacg cgtcatacag
    22201 tgcgtgaccg ccgagatgtt atcaacgccg cccagtgccg ctatcaccga gcgtgccagc
    22261 gccgcataat ttttggccat cggattttat cctgttatca gtagggtaga tgtagttgtt
    22321 atcagtcgac gtaacggaaa aatgccgatg ctgaaacgga attaggaaac cggtttcaca
    22381 aaatcatgaa tagttgtcac taaataagca agagagttaa ttgttcattt tttatatttg
    22441 tgaaaccggt cacctgaata tttttcccgc cgattgcgca aggacaatcg gcaaacggtg
    22501 cagtacactg cgttgattgc gtgtaaaggg atgaaaatga tgacgacgat gctggaagtg
    22561 gcgaagcgcg ccggggtttc aaaagcgacc gtttcccgcg tgctttcagg taatggctac
    22621 gtcagccagg agactaaaga tcgcgtgttt caggcggtag aagagagcgg ttaccgccca
    22681 aacttgctgg cgcgcaatct gtcggcgaag agtactcaga cgctggggtt ggtagtgacc
    22741 aacacgcttt accacggcat ttattttagt gaattactct ttcatgccgc gcgaatggcg
    22801 gaagagaaag ggcggcagtt gctattggca gatggtaaac acagcgccga agaagagcgc
    22861 caggcgattc agtatctgct ggatttgcgc tgcgacgcca ttatgattta cccgcgtttt
    22921 ttaagcgtgg atgagatcga tgacatcatt gacgcgcaca gtcagccgat aatggtgctt
    22981 aatcgccgcc tgcgcaaaaa cagcagccat agcgtctggt gcgatcataa acagaccagc
    23041 tttaatgccg tggcagagtt gataaacgcc ggacatcagg agattgcttt ccttaccggc
    23101 tcgatggatt cccccaccag cattgaacgt cttgctgggt ataaagacgc gctggcgcag
    23161 catggtattg cgctcaatga aaaacttatc actaacggta aatggacgcc cgccagcggg
    23221 gccgaagggg tagaaacgtt gctcgaacgt ggggctaaat ttagcgcgtt agttgccagt
    23281 aacgacgata tggcgatagg tgcgatgaaa gcgttgcacg agcgcggcgt agcggtgcca
    23341 gagcaggtgt cagttatcgg attcgatgat atcgcaattg ccccctatat cgttccggcg
    23401 ctctccagcg taaaaattcc ggtgactgag atgattcagg aaattattgg acggctgatt
    23461 tttatgctcg atggcgggga tttctcaccg ccgaaaacct tcagcggaaa actgatccgc
    23521 cgcgactcac tcattgctct ttcgcgataa acattgtcat cacatgtcat cgtcactagt
    23581 gtcgatgaca tcttttttcc tgcattacag aaattaattt ccttaaataa cagtgaatta
    23641 aaactggcat gatttgtgaa tgtatcggcg cattaactgt cattgctgga gaatttgatg
    23701 aaccgtttca tcattgctga cgcgagtaaa tgtattggtt gctgtacctg tgaagtggcg
    23761 tgcgtggttt ctcatcagga aaatcaggac tgtgcatcgc tgaccccgga aacattttta
    23821 ccgcgtatcc atgtcattaa aggtgtgaac atttccacgg cgacagtttg ccgtcagtgt
    23881 gaagatgcac cgtgcgctaa cgtctgcccg aatggtgcta tcagccgtga taaagggttt
    23941 gttcatgtga tgcaggaacg ttgcattggt tgcaaaacct gcgttgtggc ttgcccgtat
    24001 ggcgcgatgg aagtggtggt acgtccggtg attcgcaaca gcggcgcggg gctgaatgta
    24061 cgggctgaca aagccgaagc caataaatgt gacctgtgca accatcgtga agacggcccg
    24121 gcgtgtatgg cggcttgccc gactcatgcg ctgatttgtg tcgatcgtaa taaacttgaa
    24181 caactgagtg cagaaaaacg ccgccgtacg gcgctgatgt tctaaaaatc ttacagaact
    24241 gttctggcat caaaggaatt cccggaaata ttcgtagaat acggagctgg cggtatcgcc
    24301 gacctgagac tgccggatac gacgcagtcg tgtccggcag gacgggttac gcaattcatg
    24361 atggcgggaa taactcaatg gcaaaaaaca catcttgcgg tgtccaactg cgtattcgtg
    24421 gcaaagtgca gggcgtcggt tttcgtccgt ttgtctggca gctggcacag caattaaatc
    24481 ttcacggcga tgtctgtaat gacggcgatg gcgtagaagt ccggctgcgg gaagacccgg
    24541 aaacgttact tgttcaattg catcagcact gcccgccgct ggcgcgtatt gatagcgtcg
    24601 agcgtgagcc gtacatctgg tcacaactgc ccactgagtt caccatccgc cagagcgcgg
    24661 gaggtgccat gaatacgcaa attgtcccgg atgccgctac ttgccctgct tgccttgccg
    24721 aaatgaatac cccaggcgaa cggcgttatc gttatccgtt tatcaactgt acccactgcg
    24781 gcccgcgttt caccattatt cgcgccatgc cttacgaccg cccgtttacc gtgatggcgg
    24841 cgtttccgtt gtgtccggct tgtgacaaag agtaccgtga cccgctcgat cgtcgcttcc
    24901 acgcccagcc ggtggcctgc ccggagtgtg gcccacatct tgaatgggta agtcatggtg
    24961 aacatgcaga acaagaggcg gcattacagg cggctatcgc acagttaaaa atgggcaaca
    25021 ttgtcgccat caaagggatt ggcggatttc atcttgcctg cgatgcacgt aacagtaacg
    25081 cggtggcgac acttcgggcg cgcaaacatc gcccggcgaa accgctggcg gtcatgttgc
    25141 cagtggctga cggtttacca gacgctgcgc gccagttgct taccacgccc gccgcgccga
    25201 ttgtgctggt ggataaaaaa tacgttcctg agctttgtga tgatatcgcc cctgacctta
    25261 acgaagtcgg ggtaatgttg cctgcgaacc cgctccagca tttgctgtta caggaactgc
    25321 aatgcccgct ggtgatgacc tccggcaacc tgagcggtaa accaccagct atcagcaacg
    25381 aacaggcgct ggcggatttg cagggcattg ccgacggatt cttgatacat aaccgcgaca
    25441 tcgtgcagcg gatggatgat tcggtggtgc gcgaaagcgg cgaaatgctg cgccgttcgc
    25501 gggggtatgt gccggatgcg ctggctttgc ctccgggctt taaaaatgtt ccgcctgtgc
    25561 tgtgtctcgg cgcggatctg aaaaatacct tctgcctggt gcgcggtgaa caagcggtgt
    25621 tgagtcagca tctgggcgat ttaagtgacg atggcatcca gatgcagtgg cgcgaagcgt
    25681 tacgcctgat gcaaaacatc tacgatttta ccccgcaata cgttgtgcat gacgcgcatc
    25741 cgggctatgt ctccagccag tgggcgcgtg aaatgaatct gccgacgcaa acggtactgc
    25801 atcatcatgc ccatgcagcg gcgtgtctgg cagagcatca gtggccgttg gatggcggtg
    25861 atgtcattgc tttgacgctc gacggtatcg gtatggggga gaacggcgct ttgtggggcg
    25921 gcgagtgcct gcgggtgaac tatcgcgaat gtgagcacct gggcggcttg cctgcagtgg
    25981 cgcttccggg tggcgatttg gcagcgaagc agccgtggcg aaacctgctg gcgcagtgcc
    26041 tgcgctttgt gccggagtgg cagaattacc ctgaaacagc aagtgtgcaa cagcaaaact
    26101 ggagcgtgct ggcgcgggcc attgagcgtg gaattaacgc gccgctggcg tcatcgtgtg
    26161 ggcggttgtt cgatgcagtg gcggcggcac tgggctgtgc gccagccacg ttaagttatg
    26221 aaggtgaagc ggcttgtgct ctggaggcgc tcgcagcctc atgccacgga gtgacgcatc
    26281 cggtgacgat gccgctggtg gacaatcaac tggatctcgc cgctttctgg cagcagtggc
    26341 tgaactggca ggcaccggtt aatcaacgcg cgtgggcgtt tcatgatgcg ctggcgcagg
    26401 gttttgccgc gttgatgcgt gagcaggcca cgatgcgtgg tatcactacg ctggtattta
    26461 gcggcggggt tattcataac cgtttgctgc gtgcacgtct ggcgcattat ctcgctgatt
    26521 tcacattgct ctttccacag agtttaccgg cgggtgatgg cggtttgtct ctggggcagg
    26581 gggttattgc tgcggcgcgt tggttagcgg gtgaagtcca gaacggataa aacgtgttgc
    26641 atttggacac cattgccgga tgcggcgtaa acgccttatc tggcctacac atgcgtcccc
    26701 gtaggtcgga taagacgcgc cagcgtcgca tccgacattt taggcacagt agcccaccta
    26761 catcggcaat gttttcaaca atccaaatgc ctctttcatc cgatcctcac tgaccacaaa
    26821 ggcgcgaagc tggtcagcat cgtcaacacc gcgagccacc attccctggc gttcggtatt
    26881 aatttgccag cgtaaatcct gacgctgggt ttcgcctgcc agatgcagcg gtaattccgg
    26941 cgttttgatt ttcaccagca tcgccggtag tttcagcggc gtgttattgc cgagaagatt
    27001 ttttgccagc accatcgcgc taagttgaat cggctgcagg aatggcagta cctgaccgtt
    27061 gatttccgcg caatcgccca gcgcataaat atcgtcatta ctggtttgca gataactatc
    27121 gacgcaaacg ccgcgattaa tcgtcagccc ggcgcgtcgt gccagggctg tttccgggcg
    27181 cagtccggtg gcggcaatta ccgcatccac ttcgatacta cgctggtggt ccagcgttgc
    27241 cagaatgcca gaatccgttt tttccagccc ctgcaactga gatttcaaca gcagatgaac
    27301 gcccatctcc gtcaaccgat gctgcaagcg gctgcttact tctggtggca ttaatgacgc
    27361 cagaatactg gcagcgttgt cgattagcgt gaccgctttg cctgcacgac aaaaatccat
    27421 cgccagttcg ctaccaatca aaccaccgcc gacaatcaac acgcgtcggg catcccgcag
    27481 ttgcgtttca caggcgcgat actcttgctg actatttaac gtcagcatta actcacgccc
    27541 aggcactggc gggacaaagg cactggcacc agttgccagc accagcttgt cgtattgcca
    27601 ctgattattc tggcttttca ccacatgggc ttcggcatcg atatccgtca cccaggtgtg
    27661 cggaaacagg tgcagattaa actgcccggc aaattcaccc gccgtctggc gggtaaggtc
    27721 gtcggcacgt tgcccctggc tgataacatg gctgaggtcc ggtttgttgt actcatccat
    27781 gctgtcggcg gcgatcaggg ttaatggaat actggcgtcc tgtttgcgaa tatttttcac
    27841 cagttggcgg gcggcgaagc ccgaaccaat gatcacaatg ccgttgctca ttttgcctcc
    27901 gatgccagtt cgtcaaagac gtctttgccg agagagcatt ccgggcagag gaagttatcc
    27961 ggaacttcac tccacggcgt tcctggcgca acgtcctgca ttggctcgcc ttttgccgga
    28021 tcgtaaatcc actggcagac gctgcactgc atccgtgggc cgaggtcgga cttcgtggtg
    28081 gcagaggttt cttctttaac caccgtattc accgtgctct gcggcagcgg cgcgagcgcc
    28141 cactgacggg cgatttcgcg accatgttca cggcataact ccagagcgtc ctggtctggt
    28201 cgccatttgg ctttcaggct aagcgacatt tcgaaacccg catcctgcag gcgcgtggaa
    28261 agacgatcca ccgcaccgcc gctccagccg tgagagccga aagcactggc gcgtttgtta
    28321 cggaagcgta atccggtcat ctcctccacc agaccggcga ttttcggcat catcacgtta
    28381 ttcatcgtcg aagtgccgac cagcacgcct tttgagcgga agacattggt caggatttcg
    28441 tttttatcgc ttcgggcgac gttgaaaatt ttcaccgcca cgcgtgggtc ggtttccgca
    28501 atcccctggg cgatagcgtc agccatcatg cgggtgttat tcgacatggt gtcgtagaaa
    28561 atggtgattc tgtcttcctg ataatcagcc gcccatttca ggtacagctc gacaatttgc
    28621 gtcgggttat cgcgccatac cacgccgtga gaggtcgcta tcatatcgac tggtaagtta
    28681 aagcccagga tctcggtaat tttcggtgtt accaggcggc tgaacggcgt caggatattg
    28741 gcgtagtaac gctggcactg ctcgaaaagc tccgtctgat ccacttcatc gttgaacaga
    28801 tgctcgtcgc agtagtgctg accgaaagca tcgttactga acagcaccgc gtcgcctgtc
    28861 aggtaagtca tcatgctgtc cggccagtgc agcattggcg tttcgacaaa aatgagctgt
    28921 ttgccgttgc cgatatccag cgtgtcacca gttttcacca cattaaaatt ccactccgga
    28981 tgatgatgat gaccatttat cgagtcgata gcgttggcag tacagtaaat cggcgtatcg
    29041 ggaatttgcg ccatcagttc ggtcagcgcc ccagcgtggt cctcttctgc atggttaatc
    29101 acgatgtaat cgatatccgc cagatcgatt tcattacgca ggttctgcac aaattcgcgg
    29161 ctgaatttat ggtcgacggt gtcgatcagc acgttttttt cttcgcggat gaggtagctg
    29221 ttgtaactgc tgccgcgtag cgttttatat tccgtaccgt gaaaatcacg cacttcccag
    29281 tcacgttgac caacctaatg aatgttattt ttcaccacaa tagacatagc aacctcaatt
    29341 tattcagcgt gttctaaaaa gatgtcttgc taattgcaga ttacgtgcca gttttttatc
    29401 tgattgtttt tactctataa ataaaattat gctcattaat gagtagtcaa aatgactatc
    29461 aatcaaatgg tcgatatgac aatatctata gtcaaattga cagtgaggca aagatgagtt
    29521 tttccgttga tgtgctggcg aatatcgcca tcgaattgca gcgtgggatt ggtcatcagg
    29581 atcgttttca gcgcctgatc accacgctac gtcaggtgct ggagtgcgat gcgtctgcgt
    29641 tgctacgtta cgattcgcgg cagtttattc cgcttgccat cgacggtctg gcaaaggatg
    29701 tactcggtag acgctttgcg ctggaagggc atccacggct ggaagcgatt gcccgcgccg
    29761 gggatgtggt gcgctttccc gcagacagcg aattgcccga tccctatgac ggtttgattc
    29821 ctgggcagga gagtctgaag gttcacgcct gcgttggtct gccattgttt gccgggcaaa
    29881 acctgatcgg cgcactgacg ctcgacggga tgcagcccga tcagttcgat gttttcagcg
    29941 acgaagagct acggctgatt gctgcgctgg cggcgggagc gttaagcaat gcgttgctga
    30001 ttgaacaact ggaaagccag aatatgatgc caggcgatgc cacgccgttt gaagcggtga
    30061 aacagacgca gatgattggc ttgtcccctg gcatgacgca actgaaaaaa gagattgaga
    30121 ttgtggcggc gtccgatctc aacgtcctga tcagcggtga gacgggaacc ggtaaggagc
    30181 tggtggcgaa agcgattcat gaagcctcgc cacgggcggt gaatccgctg gtctatctca
    30241 actgtgctgc actgccggaa agtgtggcgg aaagtgagtt gttcgggcat gtgaaaggag
    30301 cgtttactgg cgctatcagt aaccgcagcg ggaagtttga aatggcggat aacggcactc
    30361 tgtttctgga tgagatcggc gagttgtcgt tggcattgca ggccaagctg ctgagggtgt
    30421 tgcagtatgg cgatattcag cgcgttggcg atgaccgtag tttgcgggtc gatgtgcgcg
    30481 tgctggcggc gactaaccgc gacttacgcg aagaggtgat ggcagggcga tttcgcgctg
    30541 acttgtttca tcgcctgagc gtgtttccac tttcggtgcc gccgctgcgt gagcggggcg
    30601 atgatgtcat tctgctggcg gggtatttct gcgagcagtg tcgtttgcgg ctggggctct
    30661 cccgcgtggt attaagtgcc ggagcgcgaa atttactgca acactatcgt tttccgggga
    30721 acgtgcgcga actggaacat gctattcatc gggcggtagt gctggcgaga gccacccgca
    30781 acggcgatga agtgattctt gaggcgcaac attttgcttt tcctgaggtg acgttgccgc
    30841 cgccagaagc ggcggcggtg cccgttgtta agcaaaacct gcgtgaagcg acagaagcgt
    30901 tccagcgtga aactattcgc caggcactgg cacaaaatca tcataactgg gctgcctgcg
    30961 cgcggatgct ggaaaccgac gtcgccaacc tgcatcggct ggcgaaacgt ctgggaatga
    31021 aggattaaat aatcccggcc tgatagaaat cctgcaggtt aattgcgccg gtgagtttgc
    31081 cgttttcatc caccaccggt gcggcagtga ttttgcgctt catcaggatc tctttggcgt
    31141 cgatggcgcg actttgcgcc tgtaacgtgg tgccgcctgt cgtcatcgct tcattaactg
    31201 gcgtggtgag tgcgccgccg cctaccagcc agcgacgtaa atcgccgtcg gtaaagacgc
    31261 cttgtacctg ttgttgagcg tcacataccg ccaccagccc cagaccggtg cggctgagtt
    31321 ccagcatcgc atccatcacg ctggcggtta acgccacctg tgggatggca tcgtcacggc
    31381 gcatcagatg atgcacttta ttcagcaagc gagcgcccag tgccccggct gggtgggagc
    31441 gggcaaaatc ttcttcatta aatccgcgcg cctgcatgac cgccatcgcc agcgcgtcac
    31501 ccatcatcag ggtattgacg gtgctggagg tcggcgcaag gtgcatcggg caggcttcgc
    31561 gttctacgga gatatccagc accgcttttg ccgccaggcc cagcggtgac gtcggtttgc
    31621 cggtcatcgc cagcagcgcg atagatttat cttccagacg cggaataatc agatccagtt
    31681 ccttcgcgcc accggagtaa gagataaaca gcatcacatc gcggctttca atcatcccca
    31741 gatcgccgtg cagtgcttct gccggatgga caaaaaaagc cgatgtgccg gtactggcaa
    31801 gtgttgcggc gattttctta ccaatgtggc ccgatttgcc aattcccgaa accaccactt
    31861 tgccttcgca gtgcaggatg atattggcgg cgcgaacaaa atcatcgccc agacgttccg
    31921 gtaaacggct tgcttcctgc aactccagca ttaacgtctg acgtcccgcg ttcagtagtg
    31981 cttcactcat tgctctctcc ggttatgatc acatcgatcc ctttctcttc cagcgcctga
    32041 cgaaacgccg gatcgatacc tgcgtcggta atcagcttat cgacgctttc aagactgcaa
    32101 actacgttgg ggcttttacg gccaaacttt gatgagtccg ccatcaaaat cacttcgcgc
    32161 gcggcattgc acattgcctt actgacggta taaacctcgt taaaggtggt tacgcccgca
    32221 ttgagatcga tgccgtcggt gcccataaac aatttatcga aactgaaatg ttcgaaggca
    32281 ttctctgcca gctgtccgtg aaatgaggcc gattttttac gaaacgttcc gcctggcatc
    32341 aggatagttt gttcgttatc cagttcagat agcgcattga cgatatgcag actgttggtc
    32401 atcaccgtga tgttattaaa gcgcgagagc aggggaacca tctgcaaaac ggtactgcca
    32461 gcatcaagaa tgatcgaatc gccatcatga ataaaactaa cggcagcttc tgctatcagc
    32521 tctttcttgt gggtgttgat gagtgtttta tgatcgatag gcggatcgga ttcctcttta
    32581 ttcaacacca ctccgccata agtacgaatg acggttcctg catgttccag aatgaccaga
    32641 tctttgcgaa tggttgtgcc tgtggtgtca aagtattgcg ccaattcttc aaccgagcat
    32701 ttaccctgct tttgcagata ctccagaatg gcggcctgac gctgacgagg tttcatgggc
    32761 gtgattacct gatttaggtt tcaaaatgat aacttcgcaa gcaagtaaca ttcacaacga
    32821 aattatccac gtttcagttt aagcgccaat gatagagcat tctgtgatag tggatcatgg
    32881 ggaaagatca catcgggctg taaatcaccc gcatgcatac cggtaattgc gggaattttt
    32941 tgcggtcgtg cgaagacggt cagtcctttc ataaacaagg tgtcgacgat gcgctgctga
    33001 tcgtccaggg cgatggcgac cacgacccgt ggtttaaagc gcccgctgga acggcccacg
    33061 ccaacccgcc cttgctggca tagtgtgtcg aaggcacggt taaaacgaga aatttgccag
    33121 ccgcctaagg ccagttgcgc acaccaggcg ataacggcga cggtgatgag tgcggatacc
    33181 ataacttttc cttaatgtag tggcggaagt cctgtatgcc tgatgagcgt agcgcatcag
    33241 gcattctatt agtctcacgg agatccttaa cggatctccg ctgttgatta caacatcacc
    33301 tgaccgccgg tgacattgat cgactgtccg gtgcagtacg acgccttagg actggcatag
    33361 agcagcagca tattcagcac atcctgataa tcgcagccgc gtttgagcgg tactttgtcg
    33421 atgtaatact gctcgacttg atccggtttg atacccagct tggtcgcgta ttggggtaac
    33481 agtgactgga acataggcga tttcagcagg ttaccgagca tcagtgaatg caccgtaatg
    33541 ccgtactccg ccagatccag cgccagtgat tgagtcagcc cgacgccacc aaatttcgct
    33601 gcgctgtagc cagagttgtg tttgctgccc actttgccgg atttcgagtt gatctgaata
    33661 atgcgaccct gaatcccgtc gcggatcatc aaacgagaaa attcacgggc acacaggaaa
    33721 taacccacca gattcacggt caaaatcgcc gagctggaag tcgctgataa aggctgcttt
    33781 tgctattccg gcgctgtaga ccagcaaatc cacgcgccca aagatttcat ctaccccacg
    33841 agagagcgcc aggacgcttt gctcgctggt ggcgtcagca ccaaaactat acgctgttcc
    33901 ttcaccatat tcggcgttaa tttcttgtgc cacatttgcg gctttgtcgc tctgaatatc
    33961 gacaaccgcg acgcgatacc cctcggcagc cagaccgtgg cacaggaacg cgcctaaggt
    34021 ttgcccacca ccgatgacaa cggcaacctg attcattttt tactccttaa cagattcaaa
    34081 cttcaaaacc gatcccggcg cgatatcgtc gggtacaggg cctgccacat ggacagtgcc
    34141 cggaaattcc gcttcgttta aaccatcgaa gcgcagggtg acatgaccca gttcgcgaag
    34201 gttgtcttcc gccacgctgc caacagcggt caccggatag cgatgctgcc cgagcgtaaa
    34261 ttgcaaaccg ggatggagtg cacctttcaa ctcgccgtgg caatgaatga agcaatactc
    34321 ttcgaggtcc gctggcgcgc cttcacgaaa ggtgatgagc atctggtcgc tgagggcgtc
    34381 agtggcactc gcgccgatac gggtgatggt ggtctgataa ataacggtca tgtttcagcc
    34441 tctattgata gataaaaccg gagacaaacc aggcgatcag tacagtcggt gcgccggtta
    34501 aaaagtggct caccagtaca gaagggacac cgacgcgaac cgtgtcctga cgggcttccg
    34561 ccagcgacaa accgaccggg atgaagtcgc aggccgcctg cgcgttgatg gcaaacagtg
    34621 ccggtaaagc cagatgcggc ggaatattgc cgagaccaat ctgcacgcca atcaatacgc
    34681 cgataacctg tgcgataact gcgcctgggc cgaggaaagg tgaaagcagc gggaaggagc
    34741 agatgagcgc cagcatgacc agacctagtg gatggctcgc cagcggagca agaccgtggg
    34801 caatccagtc accaaggcca gaagccataa tgatgccaat gagcgccgag acgaacgcca
    34861 taaacggcag aatggttttt aatacagtgt cgatggtgtc acgaccagat tgaaacaaca
    34921 cggcaacggc agaacccata cccattccca ccttcgccag taaaccatcg ctttgttcgg
    34981 tgattttctt gctggtgtca tagtcacggc ccacggaaga gggttgtggt gtcgcatcac
    35041 ccactacagt gatgttttct tcttttacgc cagagacata aatatcttcc acaatgtact
    35101 gcgccagcgg actggacttg cccgtcgagt ggatattaat ggtgggaata cgtcgtttcg
    35161 gatagatgcc gcagcgtaat gtgccgccac agtcgattac cgcgacacca atttccgcct
    35221 ccgcgggttc accttcttta aatccgtcaa tagcctgcca gccagtaagt tgtgccagtt
    35281 tgtcaacaat cgcaggccgg gtaccggcgg tgatatagac gatttttttg cccggcgtgg
    35341 cttccagctc aagcgggccg ccccagccac ccgttccttt ttcgatccga atacgcgtca
    35401 tgatgttgct cctgccaggt gaactttttg ttcaagttga atgcccatct ttttctcaaa
    35461 aatggcggtg gtcagatcgg tcacccagcc gcgaaagaag ttggtgacca gaccaaccag
    35521 cagatagctc accgccagtg ggccaagtgg caggttcagc gttgtcagac cgctggcaat
    35581 gccaagataa acaaacagtt cgccagggtt gatatgggga aagaggccat tcattgagtg
    35641 gcagctataa gaggctgccg cgtagtagct gggtttgtac ttttccggca taaagcgacc
    35701 caggcttaag gtcatcggat tgcaaaagac aaacgtgcca atgcacggta gcagcaggta
    35761 acgggaaaca gggttaccgg cgcaacgttg agcaaaacgt tcaatacgat gctgaccgat
    35821 aaaattaatc agtgcgttca tgataaccag caggctaatc aacagcggaa gaatgccggt
    35881 caccatcccg gtaaacacct ctccgccctt ttggaacagc ccgataaacc actctgcacc
    35941 atgagtaatg gtttctatca ttgttctctc cttcaggatt tattgtttta ttaccaaacg
    36001 gcaacctaat ctaatcagat tgaaagattt gaaagtgtta ttttgatcgc aaaatgaaag
    36061 ataaatattt taatttgaaa gtttgaataa aaggatagcg aggggaatga gttgagttat
    36121 gtaaagtccg tatcgggcag tgactaccgc ttccttgtgc ggggcgtgat gctttaccat
    36181 acttgcccct ggttgaatct gttaaatgga cccctcatgt tcaagcgtcg ttatgtaaca
    36241 ttgcttcccc tttttgtgtt gcttgccgcc tgtagcagca agccaaaacc tactgagact
    36301 gaaacgacca ccggaacgcc gtctggcggc ttcctgcttg agcctcagca caatgtgatg
    36361 cagatgggcg gcgatttcgc taataacccg aatgcccagc agttcatcga cagaatggtg
    36421 aacaaacacg gtttcgatcg tcagcagttg caggaaattc tctcccaggc gaagcgtctg
    36481 gattcggtac tgcggctgat ggataaccag gcaccaacca catcggtgaa accgccatca
    36541 ggtccgaacg gcgcatggct gcgttatcgc aaaaaattta ttacgccgga caacgtgcag
    36601 aacggtgtgg ttttctggaa tcagtatgaa gatgcgttga atcgcgcgtg gcaggtgtat
    36661 ggcgtaccgc cggaaattat cgtcgggatt atcggcgttg aaacccgctg ggggcgcgtg
    36721 atggggaaaa ctcgcatcct cgatgcgctg gcaacgctgt catttaacta cccacgccgc
    36781 gcggagtatt tctctggcga gctggaaacc ttcctgttga tggcgcgcga cgagcaggac
    36841 gatccgctca atctgaaagg ttcctttgcc ggggcgatgg gctacggaca gtttatgccg
    36901 tcgtcttaca aacaatatgc ggtagatttc agcggcgacg ggcatatcaa cctgtgggat
    36961 ccggttgatg cgatcggtag tgtggcgaac tatttcaaag cgcacggctg ggtgaaaggc
    37021 gatcaggtcg cggtaatggc aaacggtcag gctccaggct tgccaaatgg cttcaaaacc
    37081 aggtacagca tttcgcagct tgccacggca ggtttaacgc cacagcagcc gctgggcaac
    37141 catcaacaag ccagcctgct gcgtctggat gtcggtaccg gttaccagta ctggtacggt
    37201 ctgccgaact tctacaccat tacccgttat aaccacagca cccattacgc aatggcggtc
    37261 tggcagttag gacaagccgt ggcgctggcg cgcgtgcagt aactatttgc aagagggggg
    37321 cgtgagcttc cctctggcta atcagctttt ttctgaatcc tcctcgtaaa attgcaacgc
    37381 caacaccatc ttcctgacga aagtgctatc ttgtccggca taaatttttg actgactgag
    37441 gttgtgatga ctgacagtga actgatgcag ttaagcgaac aggttgggca ggcgctgaaa
    37501 gcccgtggcg caaccgtaac aactgccgag tcttgtaccg gtggttgggt agcgaaagtg
    37561 attaccgata ttgccggtag ctccgcctgg tttgaacgcg gatttgtcac ctacagtaac
    37621 gaagccaaag cgcagatgat cggcgtacgc gaagagacgc tggcgcagca tggcgcggtg
    37681 agtgaacccg tcgtggtgga aatggcgata ggtgcactga aagcggctcg tgctgattat
    37741 gccgtgtcta ttagtggtat cgccgggccg gatggcggca gtgaagagaa gcctgtcggc
    37801 accgtctggt ttgcttttgc cactgcccgc ggtgaaggca ttacccggcg ggaatgcttc
    37861 agcggcgacc gtgatgcggt gcgtcgtcag gctactgcgt atgcattgca gaccttgtgg
    37921 caacaatttc tacaaaacac ttgatactgt atgagtatac agtataattg cttcaacaga
    37981 acatattgac tatccggtat tacccggcat gacaggagta aaaatggcta tcgacgaaaa
    38041 caaacagaaa gcgttggcgg cagcactggg ccagattgag aaacaatttg gtaaaggctc
    38101 catcatgcgc ctgggtgaag accgttccat ggatgtggaa accatctcta ccggttcgct
    38161 ttcactggat atcgcgcttg gggcaggtgg tctgccgatg ggccgtatcg tcgaaatcta
    38221 cggaccggaa tcttccggta aaaccacgct gacgctgcag gtgatcgccg cagcgcagcg
    38281 tgaaggtaaa acctgtgcgt ttatcgatgc tgaacacgcg ctggacccaa tctacgcacg
    38341 taaactgggc gtcgatatcg ataacctgct gtgctcccag ccggacaccg gcgagcaggc
    38401 actggaaatc tgtgacgccc tggcgcgttc tggcgcagta gacgttatcg tcgttgactc
    38461 cgtggcggca ctgacgccga aagcggaaat cgaaggcgaa atcggcgact ctcacatggg
    38521 ccttgcggca cgtatgatga gccaggcgat gcgtaagctg gcgggtaacc tgaagcagtc
    38581 caacacgctg ctgatcttca tcaaccagat ccgtatgaaa attggtgtga tgttcggtaa
    38641 cccggaaacc actaccggtg gtaacgcgct gaaattctac gcctctgttc gtctcgacat
    38701 ccgtcgtatc ggcgcggtga aagagggcga aaacgtggtg ggtagcgaaa cccgcgtgaa
    38761 agtggtgaag aacaaaatcg ctgcaccgtt taaacaggct gaatttcaga tcctctacgg
    38821 cgaaggtatc aacttctacg gcgaactggt tgacctgggc gtaaaagaga agctgatcga
    38881 gaaagcaggc gcgtggtaca gctacaaagg tgagaagatc ggtcagggta aagcgaatgc
    38941 aactgcctgg ctgaaagata acccggaaac cgcgaaagag atcgagaaga aagtacgtga
    39001 gttgctgctg agcaacccga actcaacgcc ggatttctct gtagatgaca gcgaaggcgt
    39061 agcagaaact aacgaagatt tttaatcgtt cgttattgat gataatagat aatgtgtgca
    39121 cggacggtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccaacactg
    39181 gcgtgaaaaa ttactctcac ctcgatcctc taacgaattg gctgcacact cattgactat
    39241 taaatctgtt tatcagcaaa acctgggtcg catctgcggc ccttttgctt ttttaagttg
    39301 taaggatatg ccatgacaga atcaacatcc cgtcgcccgg catatgctcg cctgttggat
    39361 cgtgcggtac gcattctggc ggtgcgcgat cacagtgagc aagaactgcg acgtaaactc
    39421 gcggcaccga ttatgggcaa aaatggccca gaagagattg atgctacggc agaagattac
    39481 gagcgcgtta ttgcctggtg ccatgaacat ggctatctcg atgacagccg atttgttgcg
    39541 cgctttatcg ccagccgtag ccgcaaaggt tatggacctg cacgtattcg tcaggaactg
    39601 aatcagaaag gtatttcccg cgaagcgaca gaaaaagcga tgcgtgaatg tgacatcgac
    39661 tggtgcgcac tggtgcgcga tcaggcgacg cgaaaatatg gtgaaccttt gccaactgtc
    39721 ttttcagaaa aagttaagat ccagcgtttt ctgctctatc gtggctatct gatggaagat
    39781 atccaggata tttggctaaa ttttgccgac tgaacgcata cggtatttta cttcccactc
    39841 aagaaaactt atcttattcc cacttttcag ttaccagccc ggcggttaag acacgctgga
    39901 gctggtggcg atatttcgtt agcttgattt caggataatt atgagcaaga gcaccgctga
    39961 gatccgtcag gcgtttctcg actttttcca tagtaaggga catcaggtag ttgccagcag
    40021 ctccctggta ccccataacg acccaacttt gttgtttacc aacgccggga tgaaccagtt
    40081 caaggatgtg ttccttggac tcgacaagcg taattattcc cgcgctacca cttcccaacg
    40141 ctgcgtgcgt gcgggtggta aacacaacga cctggaaaat gtcggttaca ccgcgcgtca
    40201 ccataccttc ttcgaaatgc tgggcaactt cagcttcggc gactatttca aacacgatgc
    40261 cattcagttt gcatgggaac tgctgaccag cgaaaaatgg tttgccctgc cgaaagagcg
    40321 tctgtgggtt accgtctatg aaagcgacga cgaagcctac gaaatctggg aaaaagaagt
    40381 agggatcccg cgcgaacgta ttattcgcat cggcgataac aaaggtgcgc catacgcatc
    40441 tgacaacttc tggcagatgg gtgacactgg tccgtgcggc ccgtgcaccg aaatcttcta
    40501 cgatcacggc gaccacattt ggggtggccc tccgggaagt ccggaagaag acggcgaccg
    40561 ctacattgag atctggaaca tcgtcttcat gcagttcaac cgccaggcag atggcacgat
    40621 ggaaccgctg ccgaagccgt ctgtagatac cggtatgggt ctggagcgta ttgctgcggt
    40681 gctgcaacac gttaactcta actatgacat cgacctgttc cgcacgttga tccaggcggt
    40741 agcgaaagtc actggcgcga ccgatctgag caataaatcg ctgcgcgtaa tcgctgacca
    40801 cattcgttct tgtgcgttcc tgatcgcgga tggcgtaatg ccgtccaatg aaaaccgtgg
    40861 ttatgtactg cgtcgtatca ttcgtcgcgc agtgcgtcac ggcaatatgc tcggcgcgaa
    40921 agaaaccttc ttctacaaac tggttggtcc gctgatcgac gttatgggct ctgcgggtga
    40981 agacctgaaa cgccagcagg cgcaggttga gcaggtgctg aagactgaag aagagcagtt
    41041 tgcccgtact ctggagcgcg gtctggcgtt gctggatgaa gagctggcaa aactttctgg
    41101 tgatacgctg gatggtgaaa cggctttccg tctgtacgac acctatggct tcccggttga
    41161 cctgacggct gatgtttgtc gtgagcgcaa catcaaagtt gacgaagctg gttttgaagc
    41221 agcaatggaa gagcagcgtc gtcgcgcgcg cgaagccagc ggctttggtg ccgattacaa
    41281 cgcaatgatc cgtgttgata gtgcatctga atttaaaggc tatgaccatc tggaactgaa
    41341 cggcaaagtg accgcgctgt ttgttgatgg taaagcggtt gatgccatca atgcaggcca
    41401 ggaagctgtg gtcgtgctgg atcaaacgcc attctatgcg gaatccggcg gtcaggttgg
    41461 tgataaaggc gaactgaaag gcgctaactt ctccttcgcg gtggaagata cgcagaaata
    41521 cggccaggcg attggtcaca tcggtaaact tgctgcgggt tctctgaaag tgggcgacgc
    41581 tgtgcaggct gatgttgatg aggctcgtcg cgcccgtatt cgtctgaatc actccgcaac
    41641 gcacctgatg cacgctgcgc tgcgccaggt tctggggact catgtatcgc agaaaggttc
    41701 actggttaac gacaaggtgc tgcgcttcga cttctcacac aacgaagcga tgaaaccgga
    41761 agagattcgt gcggtcgaag acctggtgaa cgcacagatt cgtcgcaatt tgccgatcga
    41821 aaccaacatc atggatctcg aagcggcgaa agcgaaaggt gcgatggcgc tgttcggcga
    41881 gaagtatgat gagcgtgtac gcgtgctgag catgggcgat ttctccaccg agctgtgtgg
    41941 cggtactcac gccagccgca ctggtgatat tggtctgttc cgcatcatct ctgaatcggg
    42001 tactgctgca ggcgttcgtc gtatcgaagc ggtaaccgga gaaggcgcta tcaccaccgt
    42061 tcatgcagac agcgatcgct taagcgaagt cgcgcatctg ctgaaaggcg atagcaataa
    42121 tctggcggat aaagtgcgct cagtactgga acgtacgcgt cagttagaaa aagaactaca
    42181 acagcttaaa gaacaagctg ccgcacagga gagcgcaaat ctttccagta aggcaattga
    42241 tgttaatggt gttaagctgt tggttagcga gcttagcggt gttgagccga aaatgttgcg
    42301 taccatggtt gacgatttaa aaaatcagct ggggtcgaca attatcgtgc tggcaacggt
    42361 agccgaaggt aaggtttctc tgattgcagg cgtatctaag gacgtcacag atcgtgtgaa
    42421 agcaggggaa ctgattggta tggtcgctca gcaggtgggc ggcaagggtg gtggacgtcc
    42481 tgacatggcg caagccggtg gtacggatgc tgcggcctta cctgcagcgt tagccagtgt
    42541 gaaaggctgg gtcagcgcga aattgcaata atataagcgt caggcaatgc cgtggactcg
    42601 cttcacggca ttcgcattaa cgctatcgac aacgataaag tcaggttgaa gttgtgtata
    42661 tcggctaaac ttaggtttaa cagaatgtaa tgccatgact gcttacatgt aatgtgtttg
    42721 tcattgctta ctttttggcg ttatatgatg gataatgccg ggatacagag agacccgact
    42781 cttttaatct ttcaaggagc aaagaatgct gattctgact cgtcgagttg gtgagaccct
    42841 catgattggg gatgaggtca ccgtgacagt tttaggggta aagggcaacc aggtacgtat
    42901 tggcgtaaat gccccgaagg aagtttctgt tcaccgtgaa gagatctacc agcgtatcca
    42961 ggctgaaaaa tcccagcagt ccagttacta atctttccgc gtctcatctt tatcggtgag
    43021 acgcaccctc aaaatttctc cttcactcta tagtcttttc gctttactcc cgttcattca
    43081 acttaagtct ccattttttt gcattactgc tatctgccag acctccgttt ttctgtagat
    43141 aaaacactct ttttgacgtt tttacagact aattgagcgt gaagtgtgca aacgataaaa
    43201 gtgtgggaaa aattgtttga cttataagtc tcagaaagta atatgtgcgc cacgcagcga
    43261 cgatgagcga taaacaagtt cttcgaagca ctcgtaagag gcgtgtggtg aggtggccga
    43321 gaggctgaag gcgctcccct gctaagggag tatgcggtca aaagctgcat ccggggttcg
    43381 aatccccgcc tcaccgccat ttgcatccgt agctcagctg gatagagtac tcggctacga
    43441 accgagcggt cggaggttcg aatcctcccg gatgcaccat ctcttacttg atatggcttt
    43501 agtagcggta tcaatatcag cagtaaaata aattttcccg atgcatccgt agctcagctg
    43561 gatagagtac tcggctacga accgagcggt cggaggttcg aatcctcccg gatgcaccac
    43621 ctcttacttg atatggcttt agtagcggta tcaatatcag cagtaaaata aattttcccg
    43681 atgcatccgt agctcagctg gatagagtac tcggctacga accgagcggt cggaggttcg
    43741 aatcctcccg gatgcaccat attctccgaa atcttcagca atgaaggtat cgaagaggta
    43801 gcggaattaa ccgcaggcac cagggatgat aacgttgctt tagcaacggc ccgaagggcg
    43861 agccgcaagg cgagtaatcc tcccggatgc accatctctt aattgatatg gctttagtag
    43921 cggtatcaat atcagcagta gaataagttt tcccgatgca tccgtagctc agctggatag
    43981 agtactcggc tacgaaccga gcggtcggag gttcgaatcc tcccggatgc accatattct
    44041 ccgtaacctt cagcaatgaa ggtatcgaag agatagcggt attaaccgca ggcaccagga
    44101 aggataacgt tgcgccagca acggcccgaa gggcgaacgt cagtgagtca tcctcccgga
    44161 tgcaccatct tctccaaaaa atttccttca aaaatatctc atttctaaat aactccttta
    44221 tccatttcct gaattttctt ctccttcgcc atcagcgaca aaatcaagca attacgctaa
    44281 agtagcgtca tatttttcga cttgcgagaa gaccatgtac gagcgttatg caggtttaat
    44341 ttttgatatg gatggcacaa tcctggatac ggagcctacg caccgtaaag cgtggcgcga
    44401 agtattaggg cactacggtc ttcagtacga tattcaggcg atgattgcgc ttaatggatc
    44461 gcccacctgg cgtattgctc aggcaattat tgagctgaat caggccgatc tcgacccgca
    44521 tgcgttagcg cgtgaaaaaa cagaagcagt aagaagtatg ctgctggata gcgtcgaacc
    44581 gcttcctctt gttgatgtgg tgaaaagttg gcatggtcgt cgcccaatgg ctgtaggaac
    44641 ggggagtgaa agcgccatcg ctgaggcatt gctggcgcac ctgggattac gccattattt
    44701 tgacgccgtc gtcgctgccg atcacgtcaa acaccataaa cccgcgccag acacattttt
    44761 gttgtgcgcg cagcgtatgg gcgtgcaacc gacgcagtgt gtggtctttg aagatgccga
    44821 tttcggtatt caggcggccc gtgccgcagg tatggacgct gtggacgttc gcttgctgtg
    44881 agtgaagcgt tatcgctttt ctcgttgttt gccagtagtt ttctcagcgc tacactatta
    44941 cccggcaact ccgaagtcgt gctggtggca atgttgcttt ccgggatcag tcatccctgg
    45001 gttttagttt taacagcaac aatgggtaat agccttggag ggttaactaa cgttatcctt
    45061 gggcgtttct ttccattgcg taaaacatcg cgctggcaag agaaagctat cggctggctg
    45121 aaacgctatg gtgcagtcac gctattatta agctggatgc ccgtggttgg cgatttactg
    45181 tgtctgttag cgggatggat gcgcatctcg tggggaccgg taatcttttt tttgtgcctt
    45241 ggtaaagcgt tacgctatgt tgcagttgca gcagcgaccg ttcagggcat gatgtggtgg
    45301 cactaattgt aggcctgcac atatggtcac cattacagtt atgctaatta aaacgatttt
    45361 gacaggcggg aggtcaattt gatcccggac gtatcacagg cgctggcctg gctggaaaaa
    45421 catcctcagg cgttaaaggg gatacagcgt gggctggagc gcgaaacttt gcgtgttaat
    45481 gctgatggca cactggcaac aacaggtcat cctgaagcat taggttccgc actgacgcac
    45541 aaatggatta ctaccgattt tgcggaagca ttgctggaat tcattacacc agtggatggt
    45601 gatattgaac atatgctgac ctttatgcgc gatctgcatc gttatacggc gcgcaatatg
    45661 ggcgatgagc ggatgtggcc gttaagtatg ccatgctaca tcgcagaagg tcaggacatc
    45721 gaactggcac agtacggcac ttctaacacc ggacgcttta aaacgctgta tcgtgaaggg
    45781 ctgaaaaatc gctacggcgc gctgatgcaa accatttccg gcgtgcacta caatttctct
    45841 ttgccaatgg cattctggca agcgaagtgc ggtgatatct cgggcgctga tgccaaagag
    45901 aaaatttctg cgggctattt ccgcgttatc cgcaattact atcgtttcgg ttgggtcatt
    45961 ccttatctgt ttggtgcatc tccggcgatt tgttcttctt tcctgcaagg aaaaccaacg
    46021 tcgctgccgt ttgagaaaac cgagtgcggt atgtattacc tgccgtatgc gacctctctt
    46081 cgtttgagcg atctcggcta taccaataaa tcgcaaagca atcttggtat taccttcaac
    46141 gatctttacg agtacgtagc gggccttaaa caggcaatca aaacgccatc ggaagagtac
    46201 gcgaagattg gtattgagaa agacggtaag aggctgcaaa tcaatagcaa cgtgctgcag
    46261 attgaaaacg aactgtatgc gccgattcgt ccaaaacgcg ttacccgcag cggcgagtca
    46321 ccttctgatg cgctgttacg tggcggcatt gaatatattg aagtgcgttc gctggacatc
    46381 aacccgttct cgccgattgg tgtagatgaa cagcaggtgc gattcctcga cctgtttatg
    46441 gtctggtgtg cgctggctga tgcaccggaa atgagcagta gcgaacttgc ctgtacgcgc
    46501 gttaactgga accgggtgat ccttgaaggt cgcaaaccgg gcctgacgct gggtatcggc
    46561 tgcgaaaccg cgcagttccc gttactgcag gtgggtaaag atctgttccg cgatctgaaa
    46621 cgcgtcgcgc aaacgctgga tagcattaac ggcggcgacg cgtatcagaa agtgtgtgat
    46681 gaactggttg cctgcttcga taatcccgat ctgactttct ctgcccgtat cttaaggtct
    46741 atgattgata ctggtattgg cggaacaggc aaagcgtttg cagaagccta ccgtaatctg
    46801 ctgcgtgaag agccgctgga aattctgcgc gaagaggatt ttgtagccga gcgcgaggca
    46861 tctgaacgcc gtcagcagga aatggaaacc gctgataccg aaccgtttgc ggtgtggctg
    46921 gaaaaacaca cctgacagaa aagaaaaagg ccactcgtga gtggccaaaa tttcatctct
    46981 gaattcaggg atgatgataa caaatgcgcg tctttcatat actcagactc gcctgggaag
    47041 aaagagttca gaaaattttt aaaaaaatta ccggaggtgg ctaaatgccg ttgttagata
    47101 gcttcacagt cgatcatacc cggatggaag cgcctgcagt tcgggtggcg aaaacaatga
    47161 acaccccgca tggcgacgca atcaccgtgt tcgatctgcg cttctgcgtg ccgaacaaag
    47221 aagtgatgcc agaaagaggg atccataccc tggagcacct gtttgctggt tttatgcgta
    47281 accatcttaa cggtaatggt gtagagatta tcgatatctc gccaatgggc tgccgcaccg
    47341 gtttttatat gagtctgatt ggtacgccag atgagcagcg tgttgctgat gcctggaaag
    47401 cggcaatgga agacgtgctg aaagtgcagg atcagaatca gatcccggaa ctgaacgtct
    47461 accagtgtgg cacttaccag atgcactcgt tgcaggaagc gcaggatatt gcgcgtagca
    47521 ttctggaacg tgacgtacgc atcaacagca acgaagaact ggcactgccg aaagagaagt
    47581 tgcaggaact gcacatctag tcagtaaact atcttcacaa ttaattgaaa aaagccagtt
    47641 caaatgaact ggcttagttg tacttagtgc gcaccgccgc caccgccacc tgcgccaaat
    47701 ggcggtttag caaaccacac cagccccagc agtacgagga atatcccggc tgacatccag
    47761 aagatctcat tggcggaaat aatcagcccc tgattggtga tctgctgggc aatccagcct
    47821 gatgcctgct gttgcgtcat cccaagccct tccagttgac tgtacatcgc ctgggcattc
    47881 gggttgaacg ggtttaccga ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc
    47941 cacatggtcg tggttatcga cgtgccgata gaccccgcca gcgttcgcgt aaagttagag
    48001 aggctcgatg ccgccgccag tcgttccggt ggcaaaccag acagcgtaat ggtggtcagc
    48061 ggcataaaga agcaggccac cgcaaacccc tggataaact gcggccaggc cgacgcgcca
    48121 aaatccatac ctggttcaaa ggtataggca cgccagtaga agcagacggc atacataata
    48181 aagctgaagg ttaccagccg ccgcatatcc agtttatgcg cgaagcggcc gataatcggc
    48241 gacaggatca ccggaataat ccctaccggc gcagaggcca aacctgccca ggtcgccgtg
    48301 taaccgtaga cctcctgcaa caactgcggc agcagaacaa tagcgccgaa gtagagcata
    48361 tacgcgaggc tgatacacaa gcagccgatg gtgaagttgc gcgacttaaa caacgacaga
    48421 tcgactatcg ggttatcgtc ggtcagctcc cagacaatca ggaagcagat agccaccacc
    48481 gccaccacgg taaggatgat aatttcctgt gatgaaaacc agtccagctc tttaccgcgg
    48541 tcgagcataa tctgcaggct gccgatacca ataaccagca gtgccagccc cacggcatca
    48601 atccgccgcc gtttggtgcg ggtttcacgt ccgcgcagag tttgcagtgt catcaacacc
    48661 accgccacgc caatcggcac gttgatgaag aatatccagc cccagtggta attatcgctg
    48721 atataaccgc cgaggatcgg gccgcaaatt ggcgcgacaa tcaccgtcat cgaccacaac
    48781 gccagcgcga tcgagcgttt ggctggcggg tagttattca gcaatagact ttgcgaaagc
    48841 gggatcaacg gcccggcgac aatcccctga atcacgcgga agaagatcag catattcagg
    48901 ctgctggaga caccacacgc ccacgacgca atagcaaagg cgatggtgga ccaaaggaac
    48961 agtttcactt ccccgacgcg ctttgccagc cagccggtaa gcgggatcga gatggcattc
    49021 gccaccccga aagaagtgat tacccacgtt ccctggctga gcgatgagcc cagattcccg
    49081 gcgatagtgg ggatcgccac gttagcaatg gtggagtcca gcacctgcat gaatgtcgcc
    49141 agtgacagcg caatcgtcat aatgaccagt tgcgcgcctt ccagcggttt ttgctgttgc
    49201 atcacacgca cctctggatt agccagcgtt agcttttacg atatcgtcga tcagtttatt
    49261 gacaggtgcc aggctgattt cacgcgcggt acttaccgcg accggtgttg aacgtacttt
    49321 atttgccagt acctgaccgt cacggttagt ggtattgacg ctcaccagtg tggacaaacc
    49381 aatgcgcagc gggtattgct ccagctgttt ctggtccagt tcgatacgca caggtagacg
    49441 ctgaacgact ttgatccagt taccggtcgc attttgcgcc ggaagcagtg agaatgcgct
    49501 acctgtgccc atatccagac caaccacttt accggtgtat ttcacatcat cgccgtaaat
    49561 atccgtggtg atagtgaccg gctgaccgat acgcatattg gcaatctgcg tctctttaaa
    49621 gttggcatcc acccacatat tggtggctgg aacgaccgcc atcagcggcg tcgttgggct
    49681 aatttgcgcc ccaggctgta ccgcacggcg ggagacataa ccggtcatcg gactgacaat
    49741 acgggtacgc tccagcgcca gccaggcgtt acgtacttcg gtggcagcct gttgcacggc
    49801 aggctgatct tccagtttag tccccagaat catcgcctga ttggcattgt attgttgaat
    49861 agcgacgtcc agttgcgcct gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc
    49921 gcgaccaatc aggttggcat tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt
    49981 tgcgagggcg attttctgca cctcaatatt cgcctgcaac tgtttgctgt taatcatcag
    50041 ctggtgggtt tggcgaacgc tggaagccag tgcagttttg gctttttcaa acgcctggcg
    50101 agcatctgtc gggtcgagag tgaccagcac gtcgccttct tttacaaaat cggtgttatc
    50161 ggcccagact ttcgtcacgc tgccagacac ctgagacata atttgaatct gattccctgc
    50221 cacgtatgcg tcatcggttt cttcgaaatg acgcagtacc aaaaaccaat aaatccctat
    50281 cgctacggca ataattataa agagcaaggt gagaaggagg agcagacgct tacgtttgcc
    50341 gctcttcttt accggttgct gcggggtttg agtctccgca tttgcgctca tattgttctc
    50401 cacgatcttc ttaactcatc ggctgagccg acctgcttgc caaaaaggcc agcatgttgc
    50461 gatgctggcc ggtcattttt tcttttataa atctggattt ttgagcgaga tgacgcgtta
    50521 gctcatcgct tcgagaacca caccgtcttg ttccatctgg tcgagacggg agagcaattt
    50581 gcgggtgatt tgctcgagct gatctttttc tgttgtgctg agcgcggacc agagttgatg
    50641 caggcagtta tgctgcggtg gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa
    50701 ttgcagatgc aggcagcggc gatcgttatc gctttcacga cgttcgatcc aaccgcgttt
    50761 ttccagttca tcggcaatac gcgtcgcgtt ggtacgggat gatccaagag cacaacttaa
    50821 ttcagaaggc tgaatactgt ggttttcctg agactccagc gtaatcaacg ccataaacaa
    50881 cgtctcgtta atcccctgag ccttcagcat tttattgcgg ttctccagca gcttgctttg
    50941 catgtgcatg caaagacgag tcagaaggat ctcctgataa ggaaaatctt cgtggcggct
    51001 ggcgcgaaat tttagcattt gttcaatggg cgtaaacgaa ctatccattt gggtatgacc
    51061 tcattaattt cagccgatat agtaacgaca gtgacaaata aagtaaatgt attgttttag
    51121 aaaaatgatt cttgtgggtt atataatcgc catcactttc caggcgagcc cataggccag
    51181 cgcactaagc agtgttggga tgataatgct gcgtgtttta tagaaactgg cacccagtac
    51241 cgcgaagccg accagcgtgg gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt
    51301 agagacaacc agcagagcgc atatcgaggc gatgccaatg gtgtcgagca aaatacctac
    51361 cgcgccacgt ttggttgggc gggcattacc cacacgcagg cgcagcggca aatagcggaa
    51421 gcaataattc gccgcgccaa ccagtaaccc aggcagcaga acctcatagc tcatcgggcg
    51481 ctccttgcca gaatgcttgg attaacgcag tgaggcagcc acagacaatg cctgccagaa
    51541 tggcgacggg aatagaaaat agcgttacgc ctgcaagggc accaactaac gctgcggtaa
    51601 cgcaaagaga ttgtttgcgc tggaaagagg cgagcaggaa actcataaag agtgccggaa
    51661 gcataaaacc taatgctgct tcaacggcgg gataaccttg cagcaagccg ctgccagaga
    51721 atgcccctat taccgtacca aatacccacg atgaccatga actgaaggca atgccgatca
    51781 tccagttctc gctccagcgg cgattattgc gtaccagttt tgctgttgcg gcggcaaaaa
    51841 cctcatccgt caggccaaac gcccacaggg cggttttcga tttttgcaga cgctgaataa
    51901 tacggctacg cagtgacggg ccatacaaca catggcgaac atccattgcc atgacggtca
    51961 gtgcagcaac ccacaaacta ctcccggctg ccagcatcgc ggtaatgacg aactggctcg
    52021 cgcctgcata aatgatgcag gagaaaaaaa cgctttcgag aggagagaat cccagacggg
    52081 tcgcattcag accgaacgca aaggccaccg gaatataact aataacaatc ggtaaactgt
    52141 ctttgcatcc ttccatgaag gtcgccgaac caggagcagg ctgtggagta gggctttcca
    52201 tagagtgtac gcttaacgat tgttaatatt ataaatagac tgaatgaata tcttaacctt
    52261 atcagactga tgggcttctt aacaccctta taagtgtaaa gccacgaaaa cggttgctga
    52321 ttgcttaatt tgcggcttcc tgacgatgaa aacctcgcca ccagaccagt aagttaacta
    52381 gggcaatcgt cgcgccagcc agacaaacgc cagcccaacc gccatgttgc caggctgagg
    52441 ctgaaattag cgaaccggcg gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca
    52501 ggcgattacg cgcatcaggg tgtattcgat aaattaccgt ctggttagtg atatgcacgc
    52561 cctgtacggt gagatccagc accaggatgc cgataatcag cgccagtacg gaagtgtgac
    52621 caaaccagat cgccagccat gaaagtaata gcagcagcag accgaaagtt gtggtgtggt
    52681 gcgatttacc cttatcggca aaaccgcccg ccggacgagc gcccaacgct ccggcagctc
    52741 ccgcaagtcc aaacagacca atgacaccat cgctgtagtt aaaaggtgga gcggcaagca
    52801 aaaaggccat tgaggtccag agaatgctga agttggcaaa ggtcaggcag cccagcaatg
    52861 cgcgggtgcg gaggatctta tcgctgataa acatgctgaa aacggaacct aacaactgtg
    52921 ggtagttgag gtgggtttct gatttcattt gtggcagacc acgccataat gctagcgcca
    52981 tcagtgccat taacaccgag gcaacccaaa agacggtgcg ccagccgccg aggtttgcca
    53041 gtaacccggc aacggttcgt gccagcaaga tccccaacag cagaccgctc ataatggtgc
    53101 caaccacttt gccgcgttta tccggtgaag ccagcgtcgc tgccagcgga accagaattt
    53161 gtgccacgac tgagaataaa ccggttaatg ccgtaccgag gatcatcatc gccagcgact
    53221 gactgctggc agtgattaac atgccgcctg ccgccagtaa ggtcatcgag acaatcaggc
    53281 ggcggcgttc aaacatatca ccgaggggaa caagaaacag cagacctgct gcatagccca
    53341 actgcgcggc ggtaacaata aagcctgccg aactggcgga aagggaaaag ttacgcgcga
    53401 tggtgtcgag caatggctgg gcgtaatagt tactggctac cgcctgaccg gtggcgatag
    53461 acatcagcac gatcagcgcc gggctaagct catgattagg tttagtcatt atttttgttc
    53521 atcaggtgat gattaagctg gaaatcaata atagcggatg ttgatgaatg ggggcgaaag
    53581 atagcttgtt gaaatgtgcg atgtggtttg taggcatgat aagacgcgcc agcgtcgcat
    53641 caggcattgt gcacagaacg gcggatgcgg cgaaaacgcc ttatccgcca tacggaatga
    53701 gtgtattttg taggcatgat aagacgcgcc agcgtcgcat caggcattgt gcacagaatg
    53761 gcggatgagg caaaaacgac ttatccgccc gaataaaaat tacttctgcg ctgccagcgc
    53821 ctcattcacc cagccatcga actgctgctg gtgggctttg atccaaccat caacatggcc
    53881 ctgaatatcg ccttctgagg ctttgccgtc atgcataatg gcgttctggg cgttaatatc
    53941 tgccactggc aactgcataa tggcaaaaag tttcgctgct gccgggtttt tctcggccca
    54001 ggctttgttg gcaacgatat gcatggtgct gaccgggaag ccataattcg caccattcgg
    54061 cagtttggta tcggcgtttt tatcgcccgg cagtgcggag aacggcacct gcaaccagac
    54121 cacatctttc cctggcttca gctcattact cacccagtac ggcgtccagg tgtaataaaa
    54181 caccggtttg ccctctttgt agcgactgat ggtgtcggcc atcatcgctg cgtagttccc
    54241 ctgattatgc gtcacggtgt tggtcagttc atacgcggca agctggtggt tgatcgcacc
    54301 ttcgcagccc cagcctgggt tacagccggt taaatccgct ttaccgtcgc cgttggtatc
    54361 gaacagtttg gcgatcttcg gatctttcag ttgtgcgatg ttggtgattt tgtactggtc
    54421 ggcggttttc ttatcgatca ggtaaccctg tgccgcgccg ttaacgaata tcccttcaca
    54481 ataaaatttc ttatcgccac cggcagcttc gtacatgttg tcatgcagcg gcgtccagtt
    54541 cacggcggtg aaggttgcat cgccggaagc aagtgaggtg tagccaacgt tgtaatccac
    54601 ttcgctgggt ttattgacgg tataacctaa tttctccagt gcacgactga ccagcagcgt
    54661 ctggaaggtt tcttcagtga tggtgctctg aactggatta acagtaatgc ctttgcccgg
    54721 cagatcggct gcaaaagttt gtgtagagat aagcgtggca aacgctgtcg caaaaagtac
    54781 gctatgtcgc attgttattc ctttttttgg cagggtgata atgcccggcg ttaccgggca
    54841 agtgcagagt tacttaatga atgggcgggt cagcagaccg acagggccag tggtgtacca
    54901 gcgacggttg ccgcgactgc gtgagtcgcg cccaacggcc tgcgtcaggc ggtcgaggat
    54961 aatggcgagg atcacaatcc cgacgccgcc aacggtggca aggcccatat ccagacgacc
    55021 gataccgcga agtaccatct gacccagccc gccgacggca atcatcgagg cgatgaccac
    55081 catagaaaga gccagcatca gcgtctggtt aacgcccgcc ataatggtcg gcatcgccag
    55141 cggtaactga actttgaaca gcatctggcg cgggctggca ccgaatgagc gcgaggcttc
    55201 aatcagatcc gccggaacct ggttaatccc cagaatcgtc agacggataa tcggcggcag
    55261 agcaaagatg atcgtcacca ctacgcccgg cacgttaccg ataccaaaca gcatgacgat
    55321 tggtaccaga taaacaaacg ctggcgtggt ctgcatggca tcaagcagtg gacgaataat
    55381 tttcgccgct cgcggacttc tcgccagcca tatccccaac ggcaaaccga tgacgataca
    55441 gaacagcagg gcggttaaca ccagcgccag agtcaccatt gcctgcgacc aggcaccgat
    55501 tgcgccgatg gcaatcagcg aaaccagcgt cgccacgccc attccgaccc cggaaatctg
    55561 ccaggcgatg agagcgaaaa cgataatcgc cactggcgct ggcataccca gcagcaattg
    55621 ctggaaactg ttgaggatat aatcaaccgg aacgcgcacg ccctggaaga cgggacggaa
    55681 atgggtaaca acccagtcga tcccttcagt gacccaactg tcgagcggga tcagcgtttt
    55741 atggaacgga tcgagaatat taaaatgctc gacgtttggc gcaggcgtac tggtcagcca
    55801 gtcagcacca ccgccgtcag tcggtgcagt cgtcggtgta ccccaggcgt ctgcggattg
    55861 cgcggcactg tccgccgctg gcgtggtatc ccacggatta ttttgatcag ccattattta
    55921 ccccctcacg atctaaagcg cgcagcagca ttcctttcga aatgatgccg acatactgtt
    55981 ggtcctcgtc gaccacgggc accgcacagg gtgcctgtcc gacatgagag agcaactcgc
    56041 taagaggcgt ttgtgcatcg actgctaacg gcgcatcaat cagcgccgca tcaagacctt
    56101 gctgctgcgt taacgcggtt ttaagcgaat cgatggagac tgcgccgaca aacttattac
    56161 cgcgttcgat aacgtagcca tattcgcgat cttcatcctg caataatttc agtgccgaac
    56221 gtgggccgaa gccaggggtt ttacgaatta agccattcgg tgtccggcgg gcaatatctt
    56281 tcgcactgaa tacctgacta atatcaacgc cacggaagaa ggtacggaca taatcattcg
    56341 ccggattatt gagaatttca tccggtgtgc cgacctgtac cacttcacca ttttgcataa
    56401 tggcaattcg gtcgccaata cgcatggctt catcgagatc gtgggaaata aagacaatgg
    56461 tgcgctgatg tttcgcctgt aattttacca gctcatcctg catctcggtg cgaattaatg
    56521 gatcgagcgc cgagaaggct tcgtccatta ataaaatatc cggattaatc gctaacgcgc
    56581 gggctaatcc cacacgttga cgcatcccgc cagagagttc atccgggtag ctgtgggcat
    56641 aattttccag cccgacctga cgcagtgcat caagggcttt ttcccggcgt tcttcggcat
    56701 taattccggc caattccata ccgaacgcag tattgtccag cacggtcata tgcggcatta
    56761 aggcaaagga ctggaagacc atcgcaatct tttttctgcg cacctcacgg agtttggcat
    56821 cagatatttt ggcaatatcc acaccatcaa tcagcacttg cccgcgggtg ggttcaatca
    56881 ggcgattgag aaggcgtacc attgtggatt tacccgagcc ggataatccc atgatgacaa
    56941 atatctcgcc ttcttcaatg gccagactgg cgtcttttac gccaagcgat agcccagttt
    57001 tttccagaat ttgttctttt gaaagtcctt gttcgatata tttgaatgct cgctgtggat
    57061 gctcaccaaa tattttataa aggtttttaa tttctaattt aattgccatg caatagaaag
    57121 attcctgtta tttgtttatg tcgatatatt acccaatgga aatagttact tttttctacc
    57181 ctaacatact gagaatctga ggcaacccct gatggcaaat gtggcatgaa tattgatgcg
    57241 agcaaatcgc cggaatttcc cgtgatataa gggctgagag caaatcgaga aaagttgata
    57301 ttttttgtca aagatagcga aaaaacgcct gaatattgtg gtttcaggcg aataatgcaa
    57361 cagagtatca gtgtagatca ccacaaaata tttgcgtgat aaaaagctat ttggcgggaa
    57421 ttatttccct gtcgcgggta gtgatatttt tgaaaataac accctcagaa attccagtct
    57481 tcatcttctg tttcaaccgc tttccccatc acataagagg agcctgaacc ggaaaaaaaa
    57541 tcgtgatttt catcggcatt cggcgaaagc gcggcgagga ttgccggatt cacttccgcc
    57601 atttctgcgg gaaataaagg ttcgtagccc agattcatca aagccttatt ggcgttgtaa
    57661 cagagaaacg ctttcacatc gtcagcccac ggggtttcgg cgtacagctc atcggtgtat
    57721 tgcagctcgt tgtcgtagag ttccagcaac aaatcgaagg cgaaactctt caactcttca
    57781 cgttgtccca gagatatctt ttccatgttt ttctgatatt tatagcctat gtagtaaccg
    57841 tggactgctt catcacggat aatcagacgg atcaggtctg cggtattggt cagctttccg
    57901 cggctggaaa aatacatcgg cagccagaaa ccggaataga acaaaaaaga ttcaagaaac
    57961 acactggcga ttttcttttt cagcggatca tcaccgcgat aatgttgctg aataatctga
    58021 gcttttcgct gcaacggtgc gttttcttca ctccaggcgt aggcggcatc gacatctttg
    58081 gtctggcaca gcgtcgagaa aatcgaactg taagagcggg catgaaccgc ttccataaag
    58141 ctgatattcg ataataccgc ttcttcatga ggcgtgagtg catcgggcat cagcgaaggc
    58201 gcgccgataa cattttgcag cgtgtcgagc agcgtcaggc cagtaaaaac gcgcatcgtc
    58261 agttgttgtt ctacgacagt taatgtctgc caggcaggaa tatcgttcga cagcggcacc
    58321 ttttctggta gccagaaatt gctggtcagg cgattccaca cctccagatc tttatcgtca
    58381 gatatcttgt tccagttgat ggcgctgata cgtgagagtt tcatagatat tccttaaagt
    58441 gcacaggaga cgcagccttc aatttcagtg ccttccagcg ccatctgacg caggcgaatg
    58501 taatagagcg ttttgatacc tttgcgccag gcgtaaatct gcgctttgtt gatatcgcga
    58561 gtggtggcgg tatcggggaa aaacagcgtc agcgacaaac cctgatcgac atggcgagtc
    58621 gcttccgcgt aggtgtcgat gatcttttct gcgccgattt cgtaagcatc ctgatacagc
    58681 gccagatttt cgttagtcat aaacggggca gggtagtaaa cgcgtcctgt tttgccctct
    58741 ttgcgtatct ctactttcgc cacaatcgga tgaatactcg acgtagcatg gttgatataa
    58801 gagatagaac cggttggcgg caccgcctga agattctggt tgtaaatgcc gtagcgcatc
    58861 acgtcgtcgc gcagttgcgc ccacatctca cgggtaggta acgtaatacc gctgcgggca
    58921 aacagttcgc caactttcgc cgttttcggc tgccagttac cttgcagata ttggctaaaa
    58981 tattcaccac tggcataacg tgactgtttg aacccggcga aggtttcacc gcgttcgcgc
    59041 gccagcaaca tcgaggtacg cagtgcgtgc caggtgatgg catagaaata gagattggtg
    59101 aaatccagtg cttccggcga accataagcg atgccttctc gcgccagata gccgtgtaaa
    59161 ttcatctgcc ccagtccgat ggcgtgtgag gcggcatttc cggcttcgat ggacggcacg
    59221 ctgcggatat gactcatatc tgacaccgcc gttaaaccgc gcacggcagt ctctaccgtg
    59281 cgggcaaaat cgggggaatc catggtgtgc gcaatattca acgaacctaa attgcaggag
    59341 atatcatggc ctgtgcgggc atagtcgaga ttctcgtcat actctgaggc acagttgacc
    59401 tgcaaaatct ctgagcagag attactcata tttatgcgcc cggcgacagg gttagcacgg
    59461 tttaccgtgt cttcatacat gatgtaggga tagccggact caaactggat ttctgccagc
    59521 cgctggaaga aatcacgggc gttgaggtat tttttgcgga tgcgttcatc ggcaaccagt
    59581 tcgtcatagt gttcgctgat ggcgatatcg gcaaacggct tgccataaac tcgctcaacg
    59641 tcatagggcg aaaacagcgc catctgcgca ttctcttttg ccagatggaa agtgatatcc
    59701 gggatcacca cgcccagcga cagtgtttta atgcggattt tttcgtcggc attttcccgt
    59761 tttgtatcga gaaaacgcag aatatcggga tgatgagcat gtaaatagac tgcacctgcc
    59821 ccctgacgcg cgccgagttg gttggcatag gaaaatgcgt cttccagcat tttcatcacc
    59881 ggaattacgc cagaagattg attttcaata cgtttaattg gcgcgcccgc ttctcgcaga
    59941 ttcgacagca aaaatgccac gccgccgccg cgtttcgaca gttgcagtgc ggaatttacc
    60001 gcccggccaa tcgactccat attgtcttca atacgcagca gaaaacagga aaccagttcg
    60061 ccgcgctgct gcttaccgca gttgaggaat gtcggcgtgg ctggctgaaa gcgtcctgac
    60121 agcatttcat cggtcagttg caacgccagc gtcgcatcgc cttgtgccag cgtcagcgcc
    60181 accatcgtga ctcgatcggc aaaatcttcc agataacgtt taccgtcgaa tgtcttcaac
    60241 gtatagctgg tgtaaaactt ccatgccccg aggaatgtct ggaaacgaaa accgctggtg
    60301 tgtgcgtggg caaacagcgt aatgacaaag tcgcgagagt agcgattaag aacgctttca
    60361 tcatagtaac cttcgttgac cagccagttc aggcgctgct gctggctact gaaggtcaca
    60421 ctgttcggac gcacatgcgt cgccataaag gcgtcaacgg cctggcggtc tttatcgaac
    60481 tgaatgcgac ctgcgctatc gtagaggtta agcatcgcat tcagcgcgtg gtaatccatc
    60541 gtttcctgcg tcaggcattc tgcggttgtc gttgccaaaa ttcggttact cctttacgaa
    60601 cgttttcgat atcgctttgc gtacccatga gttcaaaacg gtacagccac ggcacgccgc
    60661 atttccgggc aatcacatct ccggcgcggc catacgcctc accaaagttg cgattaccag
    60721 aagcaataac gccgcgaagc aacgcccggt tgtgctcgtc gtttaaaaag cgaattacct
    60781 atcgtggcac cgcgccagcc gtaccgccgc cgccgtaaga gggcacgatc aggatgtaag
    60841 gctcgtctac ctgaatccgt tcccgctcat tgagcgggat gcgcaccgcg ggcagaccta
    60901 aacgttcgat aaaacgctgc gtgttttcgg agctgctgga gaagtagacg agctggctca
    60961 tgcactggcc gcgtgtggcg ctggatgcag acggttaatc atgtccggac ggaaaccaga
    61021 ccagctaaga tcgccagcaa tcactaccgg caactgacga aagccctgag cacgcaacgc
    61081 ttccgctgct tcaggaacgc gatcgacatt aatcatttca aaatcaaagc cccggttttc
    61141 catcgcccgt ttggtggcgt ggcactgaac gcaatcgtta cgagtgtaaa tagttatgcg
    61201 catgattcgt atttccgttt aaaatgaaga tacggcgcga tgatacgcgt cgggttgtct
    61261 ctctgttgat acagagatac tagatgtagt tgaaaaaaga ttcaaccaca caatatatag
    61321 caaatagtga tcgaaattac cctagatatg agcgtgacgg gggaggggga tttttgtgat
    61381 tcaccaggca aaaagaaacc ccgaagacag gcttcggggt taaagacgcg tatttattat
    61441 catttttgca ctacgatttg cgcatgctta acagtgcgcc gataaaaata cctaccgcag
    61501 ctgctgtacc cacgctacac cagggtcttt cacgaacaaa agaatccgcg cagccaacgg
    61561 catcgcgtgc ggcttgctgg acgcgagtac gaccatgcat tcgcgccctg gtttctttca
    61621 gcaatgcctg agctttactg cgtgcggctt cagcttcccc tttggcgtcg ctgccccagg
    61681 acttcaatac agattcgagg ctgtcagcta attgattgac atcattctgg atatcctgca
    61741 cgccatcatc aacgtcgttg cggttcggtc ggttaaacat atgatccccc attagaattc
    61801 gatgtaattt tcagtgtaga ccataatttt cttaactgag gttcatcgcg gctttatgga
    61861 cttttctgaa agcgttgtga atgcgcaatc aggtaaggta ggcccgcggt tgataataac
    61921 gaggatcaaa tatgtattta cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt
    61981 ttactgtcga tgtggtaacc cagaaagctt atggttaccg ccgtggggaa aattatgtct
    62041 atgttaatcg cgaagcgcga atgggacgta ccgcactggt aattcatcca acattaaaag
    62101 aacgtagttc gacactggct gaacccgctt ccgatattaa aacttgcgat cattatcaac
    62161 aatttccgct ctatttagca ggcgagcgac acgagcatta cggtatcccg catggcttca
    62221 gttcgcgtgt tgcgcttgaa cgttatttga atggtttatt tggcgaagcc agttaaagac
    62281 gcgactggcg atgccagtcg cgaaaagaag agtcaggctt ttacctgctg gtaatggctg
    62341 actttaaaca gtcggcggca ataatcgagg aagtagccat aaaccgcccc catcaacatc
    62401 gaaacaacga tgtttgaact gaccgccgcc ataatctgat gccagtctgc gcccaccact
    62461 aacaagatcg ccacatacac cggtgactgg aacgtcacat aagccaggat atcagccaga
    62521 ttttttatcc agcccgacgg gctaactttg cgtgccgcgc gcataaatag atcgcggtac
    62581 ataccgtatg gccatgcaat taagatgttc accggaatcg ctaccaatct ggaataaaaa
    62641 gactgttcga agctcattcc ggagaggaaa acttcaatac acatgttcac gacagaacag
    62701 taaacaacca tcgcgaacgt atctgcaact gcatgacgca agcgtgactg cggtgagaac
    62761 atgctaatgc tccttaataa aagcgtaatg ggatgttaat ggagatgaaa gcagtggatc
    62821 tcattgccat ctgcattttc gcatagcgta tatcgctggc tatgactatt caattagtga
    62881 aaaattttta tttagtcagt ttttgtagat aaaatattcc gtaacggctc gttttttgta
    62941 cattgtggat ttttacatgc tgatatgatt tattttcaat taaatcaatt agatatgacc
    63001 ttgcctttaa tttcactaat gaaagtttgg gggtgaggta acgctataag cgtagagaat
    63061 tcggtgtatg tcgtgaccgt gtcatttaca gcatttttga tgccagaaaa tgtacattaa
    63121 cagcagaaag caccagtgag gggggggccc ctattaagtt gtcaagcatg ttatgacccc
    63181 tgcggggtat aaaaagtccc gtcgcgcatc atggcgaaca gaacgtcgca g
//
LOCUS       DAJTVK010000008        60960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000008 DAJTVK010000000
VERSION     DAJTVK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.008"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..468
                     /locus_tag="OO372_000554"
     CDS             <1..468
                     /locus_tag="OO372_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6340146.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="OO372_000555"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="OO372_000555"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6340147.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="OO372_000556"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="OO372_000556"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6340148.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OO372_000557"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="OO372_000557"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6340149.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="OO372_000558"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="OO372_000558"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6340150.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="OO372_000559"
     CDS             complement(4084..5313)
                     /locus_tag="OO372_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6340151.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="OO372_000560"
     CDS             complement(5377..5817)
                     /locus_tag="OO372_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6340152.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="OO372_000561"
     CDS             complement(5922..6692)
                     /locus_tag="OO372_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6340153.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="OO372_000562"
     CDS             complement(6689..7615)
                     /locus_tag="OO372_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6340154.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="OO372_000563"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="OO372_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6340155.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OO372_000564"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="OO372_000564"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6340156.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="OO372_000565"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="OO372_000565"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6340157.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VSAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OO372_000566"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="OO372_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6340158.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="OO372_000567"
     CDS             13322..13669
                     /locus_tag="OO372_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6340159.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="OO372_000568"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="OO372_000568"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6340160.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="OO372_000569"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="OO372_000569"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6340161.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="OO372_000570"
     CDS             complement(15489..15851)
                     /locus_tag="OO372_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6340162.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OO372_000571"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="OO372_000571"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6340163.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="OO372_000572"
     CDS             18978..20735
                     /locus_tag="OO372_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR6340164.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OO372_000573"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="OO372_000573"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6340165.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OO372_000574"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="OO372_000574"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6340166.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OO372_000575"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="OO372_000575"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6340167.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OO372_000576"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="OO372_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6340168.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="OO372_000577"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="OO372_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6340169.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OO372_000578"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="OO372_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6340170.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OO372_000579"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="OO372_000579"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6340171.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="OO372_000580"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="OO372_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6340172.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="OO372_000581"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="OO372_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6340173.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="OO372_000582"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="OO372_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="OO372_000583"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="OO372_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OO372_000584"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="OO372_000584"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6340174.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36855..36899)
                     /locus_tag="OO372_000585"
     CDS             complement(36855..36899)
                     /locus_tag="OO372_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340175.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="OO372_000586"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="OO372_000586"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6340176.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="OO372_000587"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="OO372_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6340177.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="OO372_000588"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="OO372_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6340178.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OO372_000589"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="OO372_000589"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6340179.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OO372_000590"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="OO372_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6340180.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OO372_000591"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="OO372_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6340181.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OO372_000592"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="OO372_000592"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6340182.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OO372_000593"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="OO372_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6340183.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OO372_000594"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="OO372_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6340184.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OO372_000595"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="OO372_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6340185.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OO372_000596"
     CDS             complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="OO372_000596"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6340186.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OO372_000597"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="OO372_000597"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6340187.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OO372_000598"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="OO372_000598"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6340188.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OO372_000599"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="OO372_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6340189.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OO372_000600"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="OO372_000600"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6340190.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OO372_000601"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="OO372_000601"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6340191.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OO372_000602"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="OO372_000602"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6340192.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OO372_000603"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="OO372_000603"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6340193.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OO372_000604"
     CDS             complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="OO372_000604"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6340194.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OO372_000605"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="OO372_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6340195.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OO372_000606"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="OO372_000606"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6340196.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OO372_000607"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="OO372_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6340197.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16855 c        14999 g        14510 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggtttcggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacgcg
//
LOCUS       DAJTVK010000009        59841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000009 DAJTVK010000000
VERSION     DAJTVK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.009"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..262)
                     /locus_tag="OO372_000608"
     CDS             complement(<1..262)
                     /locus_tag="OO372_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6340198.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
     gene            369..665
                     /locus_tag="OO372_000609"
     CDS             369..665
                     /locus_tag="OO372_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6340199.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO372_000610"
     CDS             complement(799..1224)
                     /gene="arsC"
                     /locus_tag="OO372_000610"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR6340200.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO372_000611"
     CDS             complement(1237..2526)
                     /gene="arsB"
                     /locus_tag="OO372_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6340201.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO372_000612"
     CDS             complement(2580..2933)
                     /gene="arsR"
                     /locus_tag="OO372_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6340202.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(3472..3645)
                     /locus_tag="OO372_000613"
     CDS             complement(3472..3645)
                     /locus_tag="OO372_000613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340203.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            3673..3756
                     /locus_tag="OO372_000614"
     CDS             3673..3756
                     /locus_tag="OO372_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6340204.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO372_000615"
     CDS             complement(3810..5162)
                     /gene="gorA"
                     /locus_tag="OO372_000615"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6340205.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO372_000616"
     CDS             complement(5234..6076)
                     /gene="rlmJ"
                     /locus_tag="OO372_000616"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6340206.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6279..8321
                     /gene="prlC"
                     /locus_tag="OO372_000617"
     CDS             6279..8321
                     /gene="prlC"
                     /locus_tag="OO372_000617"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6340207.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO372_000618"
     CDS             8329..9081
                     /gene="rsmJ"
                     /locus_tag="OO372_000618"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6340208.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO372_000619"
     CDS             complement(9130..10599)
                     /gene="dtpB"
                     /locus_tag="OO372_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR6340209.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO372_000620"
     CDS             complement(10917..11351)
                     /gene="uspA"
                     /locus_tag="OO372_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6340210.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11742..12077
                     /gene="uspB"
                     /locus_tag="OO372_000621"
     CDS             11742..12077
                     /gene="uspB"
                     /locus_tag="OO372_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6340211.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO372_000622"
     CDS             complement(12321..13820)
                     /gene="pitA"
                     /locus_tag="OO372_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6340212.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14052..15254
                     /locus_tag="OO372_000623"
     CDS             14052..15254
                     /locus_tag="OO372_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6340213.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15570..16553)
                     /locus_tag="OO372_000624"
                     /pseudo
     CDS             complement(15570..16553)
                     /locus_tag="OO372_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            16953..18560
                     /locus_tag="OO372_000625"
     CDS             16953..18560
                     /locus_tag="OO372_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6340214.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            18822..20444
                     /locus_tag="OO372_000626"
     CDS             18822..20444
                     /locus_tag="OO372_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6340215.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            20810..21877
                     /locus_tag="OO372_000627"
     CDS             20810..21877
                     /locus_tag="OO372_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6340216.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            21874..24609
                     /gene="rbbA"
                     /locus_tag="OO372_000628"
     CDS             21874..24609
                     /gene="rbbA"
                     /locus_tag="OO372_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6340217.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            24609..25733
                     /locus_tag="OO372_000629"
     CDS             24609..25733
                     /locus_tag="OO372_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6340218.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            25806..26079
                     /locus_tag="OO372_000630"
                     /pseudo
     CDS             25806..26079
                     /locus_tag="OO372_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            26076..26435
                     /locus_tag="OO372_000631"
     CDS             26076..26435
                     /locus_tag="OO372_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6340219.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO372_000632"
     CDS             complement(26555..26956)
                     /gene="nikR"
                     /locus_tag="OO372_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6340220.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO372_000633"
     CDS             complement(26962..27768)
                     /gene="nikE"
                     /locus_tag="OO372_000633"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6340221.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO372_000634"
     CDS             complement(27765..28529)
                     /gene="nikD"
                     /locus_tag="OO372_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6340222.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO372_000635"
     CDS             complement(28529..29362)
                     /gene="nikC"
                     /locus_tag="OO372_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6340223.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO372_000636"
     CDS             complement(29359..30303)
                     /gene="nikB"
                     /locus_tag="OO372_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6340224.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO372_000637"
     CDS             complement(30303..31877)
                     /gene="nikA"
                     /locus_tag="OO372_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6340225.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO372_000638"
     CDS             complement(31988..32575)
                     /gene="acpT"
                     /locus_tag="OO372_000638"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6340226.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(32630..33679)
                     /locus_tag="OO372_000639"
     CDS             complement(32630..33679)
                     /locus_tag="OO372_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6340227.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            33811..35028
                     /locus_tag="OO372_000640"
     CDS             33811..35028
                     /locus_tag="OO372_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6340228.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO372_000641"
     CDS             complement(35032..35589)
                     /gene="dcrB"
                     /locus_tag="OO372_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6340229.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(35662..36327)
                     /locus_tag="OO372_000642"
     CDS             complement(35662..36327)
                     /locus_tag="OO372_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6340230.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            36548..36793
                     /gene="tusA"
                     /locus_tag="OO372_000643"
     CDS             36548..36793
                     /gene="tusA"
                     /locus_tag="OO372_000643"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6340231.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO372_000644"
     CDS             complement(36895..39093)
                     /gene="zntA"
                     /locus_tag="OO372_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6340232.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39167..39793)
                     /locus_tag="OO372_000645"
     CDS             complement(39167..39793)
                     /locus_tag="OO372_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6340233.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            39934..40293
                     /locus_tag="OO372_000646"
     CDS             39934..40293
                     /locus_tag="OO372_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6340234.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(40296..40565)
                     /locus_tag="OO372_000647"
     CDS             complement(40296..40565)
                     /locus_tag="OO372_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6340235.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO372_000648"
     CDS             complement(40555..41151)
                     /gene="rsmD"
                     /locus_tag="OO372_000648"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6340236.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            41301..42797
                     /gene="ftsY"
                     /locus_tag="OO372_000649"
     CDS             41301..42797
                     /gene="ftsY"
                     /locus_tag="OO372_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6340237.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            42800..43468
                     /gene="ftsE"
                     /locus_tag="OO372_000650"
     CDS             42800..43468
                     /gene="ftsE"
                     /locus_tag="OO372_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6340238.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            43461..44519
                     /gene="ftsX"
                     /locus_tag="OO372_000651"
     CDS             43461..44519
                     /gene="ftsX"
                     /locus_tag="OO372_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6340239.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            44764..45618
                     /gene="rpoH"
                     /locus_tag="OO372_000652"
     CDS             44764..45618
                     /gene="rpoH"
                     /locus_tag="OO372_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6340240.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            45889..46992
                     /gene="livJ"
                     /locus_tag="OO372_000653"
     CDS             45889..46992
                     /gene="livJ"
                     /locus_tag="OO372_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6340241.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO372_000654"
     CDS             complement(47191..47574)
                     /gene="panM"
                     /locus_tag="OO372_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6340242.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            47998..49107
                     /gene="livK"
                     /locus_tag="OO372_000655"
     CDS             47998..49107
                     /gene="livK"
                     /locus_tag="OO372_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6340243.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            49155..50081
                     /gene="livH"
                     /locus_tag="OO372_000656"
     CDS             49155..50081
                     /gene="livH"
                     /locus_tag="OO372_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6340244.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            50078..51355
                     /gene="livM"
                     /locus_tag="OO372_000657"
     CDS             50078..51355
                     /gene="livM"
                     /locus_tag="OO372_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6340245.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            51352..52119
                     /gene="livG"
                     /locus_tag="OO372_000658"
     CDS             51352..52119
                     /gene="livG"
                     /locus_tag="OO372_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6340246.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            52121..52834
                     /gene="livF"
                     /locus_tag="OO372_000659"
     CDS             52121..52834
                     /gene="livF"
                     /locus_tag="OO372_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6340247.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            53118..54182
                     /gene="ugpB"
                     /locus_tag="OO372_000660"
                     /pseudo
     CDS             53118..54182
                     /gene="ugpB"
                     /locus_tag="OO372_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            54280..55167
                     /gene="ugpA"
                     /locus_tag="OO372_000661"
     CDS             54280..55167
                     /gene="ugpA"
                     /locus_tag="OO372_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6340248.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            55164..56009
                     /gene="ugpE"
                     /locus_tag="OO372_000662"
     CDS             55164..56009
                     /gene="ugpE"
                     /locus_tag="OO372_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6340249.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            56011..57081
                     /gene="ugpC"
                     /locus_tag="OO372_000663"
     CDS             56011..57081
                     /gene="ugpC"
                     /locus_tag="OO372_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR6340250.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO372_000664"
     CDS             57078..57821
                     /gene="ugpQ"
                     /locus_tag="OO372_000664"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6340251.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(57808..58248)
                     /locus_tag="OO372_000665"
     CDS             complement(57808..58248)
                     /locus_tag="OO372_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6340252.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            58368..>59841
                     /gene="ggt"
                     /locus_tag="OO372_000666"
     CDS             58368..>59841
                     /gene="ggt"
                     /locus_tag="OO372_000666"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6340253.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT        14594 a        15113 c        16139 g        13995 t
ORIGIN      
        1 acccggcttt cgctgacaac aatccacgta acatgggtat tgtcgagcaa ctacagaacg
       61 gggtaacggc tcccaacaga gcagccagga gatagcctct cccttttctg gcccccatca
      121 tctgctggat tttatggtct ggcacctttt gtcttatcag gctgacaccg gcactaatca
      181 ctataaacag caacgaaagc tcgacggcga gaaacacaaa catctctgcg gcatcttgca
      241 gcatcgcaag ccaactactc atacgtaacc tcattggata aatctatatt tccagaataa
      301 tcgaaatgaa attcagatca agatatttct ggtattatcg aaatatcaac cagacagagg
      361 ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa agagctaggt cacccgaccc
      421 gtttgttcat atttaaacat cttgtcaaag ccggggaaca agggttgccg gtcggtgagt
      481 tgcaaaagca gcttgggata cctggctcca cactcagcca ccatatatca gcactggtat
      541 cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct gatgtgtgtt tcgcaatacg
      601 caatgctgga agcgataatt gaattcttac gcgaggagtg ctgcgtaaat agcaaaattg
      661 cctgatgcgc tacgctctac atatttcatg ccatatattg aatttgcacg gttttgtagg
      721 tcggataaga cgcgttagcg tcgcatccga cataaacaaa gcgcacttta ccaactatcg
      781 gcagtggata tcgccgcttt atttcaggcg cttacccgct tcatcaacca ctttctcgcc
      841 atcttccttg gagaatgcgc ctttttgcgc atctggcaga atttccagca ccacttctga
      901 agggcggcac aggcgagttc ccagcggcgt caccacaatc gggcgattaa tcagaatcgg
      961 gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat ttatcttccg caaggcccag
     1021 ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt acggaaatcc ccatatcggc
     1081 aatgagtttg accagttcat cgcgcgttgg cggagtttcc agataatgga taatggtcgg
     1141 ttctgtgccg ctgttgcgga tcatctccag cgtattacgc gacgtgccgc aggccgggtt
     1201 gtgataaatg gtaatgttgc tcatatcagt atctcattac aaagtgaaag agagacgtag
     1261 cgccagcgca gccagcgtca caaacagcac aggcagggtc atgataatcc ctgtacggaa
     1321 gtaatatccc cagctgatag tcatattctt ctgcgaaagt acgtgcagcc agagtagcgt
     1381 agccaggcta ccaattgggg taattttcgg tcccaaatcg cagccaatca cattggcata
     1441 aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca acgcgccaac
     1501 cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga atccggtgcc
     1561 gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag aaagatattc
     1621 cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg agaagatgac
     1681 aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac cgcgtttagc
     1741 gacgacaaat aatatcagcg cgcccacagc tgcaatggcg ctcaccggaa tgccgagcgg
     1801 ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag ttttgaacgt
     1861 agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt agttctgcgg
     1921 aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg cggcgatatc
     1981 caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat cagcggaaac
     2041 gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa tgaatccggc
     2101 cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca gcatggcgat
     2161 gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggcgg caacggcagc
     2221 accgagcagg acaatccagg taaacagcaa gcgaccacga ccattacccc agcgtgagac
     2281 gtgcagcgcc gcccattcaa aaaagccgga ctcatccagc agcaggctga tgataatgac
     2341 ggcgataaac gcagccgtcg cgttccagac gatattccac accaccggaa tatcacccgg
     2401 atgaaccacg cccgtaacta acgccagtac tgcgccgagc gttgcactcc agccgatgcc
     2461 taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag cgcctgccag
     2521 taacataatg cctcccggat aaaacacatc tgaaaattca tatgtgttta gctaaatttt
     2581 taactgcaaa tgttcttact gtccccggaa cagttttgtc gagccaggtt gcggacaatc
     2641 gcctgaacct tttcctgttc acatcgccag gcctcatcaa taattttcgc cgcccatgct
     2701 ggaatatgcg gtgataagcg gtaatgaacc cacttacctt gcttgcggtc cagcaatagc
     2761 ccgctttcac gaagcaatgc caggtggcgg gagatcttgg gctgcgactg atcgagggca
     2821 gtacaaagat cgcagacgca taactctccc agctcgctga gcagtaaaac gatgcccaga
     2881 cgggtttcat cagcaagaat tttgaacaat tggatgggta acagaaatga catattgcgc
     2941 tcctgattgt ggcaggtagt gtctctcttc gaagcggata agtcaaaaac atatatgact
     3001 taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa ttttgagttg gctattcatt
     3061 tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt gcgtcggttt tttactcttc
     3121 agcctgagtt ggtggctctg gctggagtga gaatgcccac atctaacggt tcaatactat
     3181 tagccagtgg ctaacattgc aagaattagc gatttcttca gctggcggag ggggcacttc
     3241 ggtatatatc gcaatgacga tttatatact ttcactgggt catcgtcata ttaagccttg
     3301 tccgaatccg gatgtaaaaa aaccgacttt gcgtcggttt ttttactctt cagccctgag
     3361 ttggtggctc tggctggagt gagatagtca tcatctaacg catcaatgct aataccatac
     3421 tgaaactatt gcaaggacat gctggtttta taacctgaat gtactgtatg attatccagt
     3481 tagctctgag gcattttcac tctggtaatg cgcataaacg ctttcaaagt cctggtcaga
     3541 agtacgggtg gtgccgttaa ctgatgctct ggccggagtg agatagtcgt catctaacaa
     3601 tgagacatgc gccgtgacag gcagtggatg agtaagcgga tgcattctca ctccatcgca
     3661 tggagaaaac gggtgattga taaagcaatc atcgttcttg gagcgttaat tgcgctgctg
     3721 gaactgatcc gctttctgct tcagcttctg aactgatagc ggaaacgtaa ttaagggcta
     3781 agagcacact actcttagcc ctttaacatt tagcgcatgg tcacgaactc ttctgccgcc
     3841 gttgggtgaa tggcgacggt attgtcgaag tcttttttgg ttgcgcccat cttcagcgcc
     3901 accgcgaagc cctgcaacat ttcgtccata ccaaagccaa tgccgtgaat accgacaatc
     3961 ttctcttctg gcccaacgca caccagcttc atgcggcacg gctggcggtg agtggtgacg
     4021 gcggtataca tcgcggtgaa agaggattta tacactttca cctgatcgtc gccgtactgc
     4081 tcgcgcgcct gcggttccgt taaaccaaca gtaccaatcg gcggatggct gaagaccacg
     4141 gtcggaatgt tgctgtaatc cagatgctca tccggcttgt tattaaacag gcgttcagag
     4201 agacggcgac ccgctgcaac tgctaccggt gtcagttcca ctgcacccgt gttatcgccc
     4261 accgcgtaaa taccttcaat attggtgttt tgatatttat cgacgacgat atagcctttt
     4321 tcgttagttt taacgccagc ggcttctagg ttgatgttgt cattggcagg ctcgcgacca
     4381 atcgcccaaa tcaggcaatc caccgtttca ctgcgaccat cttccagctc cagcgtcagg
     4441 ctaccatcgg catttttcac taccgctttc gggatggcgt tggtgtgcag ctgcgggcct
     4501 tcggcgttca tcacttcgac cagcgtttcg gaaatcatcg ggtcgaagct gcgcagcggc
     4561 gcatgtttac gcacaaacag atgcgttttc gcgccgaggc cgttaatcac gcccgccagc
     4621 tcaacggcga tgtaacccgc gccaacaacc gccacgcgct ctggcaaagc aggaagggcg
     4681 aagaagccat cagaatcaat accgtattcc acgcccggaa tatccgggtg gctcggacga
     4741 ccgcctgtgg cgatcagaat atgatcggcc gtgatggttt cgccgtttac ctccagcgtt
     4801 ttggcatcaa cgaagcgggc aaagcctttg attacatcaa cgttattttt accgagcacg
     4861 ttttcatagg aagtatgaat acggtcgata taggcggtac ggctggcgat caacgtttcc
     4921 cagttgaact tgttgatggt ggtatcgaaa ccataatcag ggccgtacat atggatcgct
     4981 tcacggattt gcgccgcgtg ccacatcact tttttcggca cgcagccgac gttcacgcag
     5041 gtgccgccca gctctttggc ttcaatcagt gcgcatttct ggccgtacat agccgcgcgg
     5101 ttgatggagg cgataccgcc gctgccgccg ccgatggcga tgtaatcata gtgtttagtc
     5161 atgacagagt gtccttatcg ttgattaccg cgattgtagc gcgagacgta ataggtgcca
     5221 gcaatggctg caattactcc ggcacgatcc agcttacggt ggcgtgcccg gtgcctgccg
     5281 gaaccagttt gctgtgcagc cacggcagca cgttattcat ctgctgttcc agtttccacg
     5341 gcgggttaat cacaatcatg ccggaagcgg tcatgccacg gcgatcgctg tctggcagta
     5401 ccgccagttc aatttgcaga attttgcgaa taccggtcgc ttccagatcg tggatcatgc
     5461 gcttaatttg ctgacgcagc accaccggat accacagtgc gtaagtacca gtggcgaaac
     5521 gtttgtaacc ttctgctatc ccgctgacca ccgcctgata gtcggttttc atttcatacg
     5581 gcgggtcgat aaggattaaa ccacggcggg aaaccggcgg aagtttggcc ttaagctgct
     5641 ggaaaccgtc ggctttttcg acgcgcgcac ggctgtcttt ctgaaattca gaacgcagca
     5701 acgggtaatc gctcgggtgc agttcggtca gttgcaggct gtcttgttcg cgcagtagct
     5761 ggcgagcaat caacggcgac ccggggtagt aacgcaactg accgctacgg ttgaagtgtt
     5821 ttaccacatt gatgtacgcc tccagttctg cgggcaaatc gtcctgctgc cagatacggg
     5881 cgataccttc gagatattcg ccggtacgct cggcatgttc gctgcctaac tgataacgcc
     5941 cggcccctgc gtgggtatcg agatagagaa acggtttatc tttctctttc agcgactcga
     6001 tgatcaggct ctgaacggta tgtttaagga cgtcggcgtg gttgccagcg tgaaagctgt
     6061 ggcgataact gagcatgggt aaaggtgttc cggtaagtaa aaatcggccc gtattcgggg
     6121 cgcacaaaag cgtatcagga cagtataccg aaaagaggcc gccgccgcga aagcgtaacg
     6181 tttctcattg aaattcacta cacttaaccc catgctacac acattatgta aagcgcctgt
     6241 tgagcgcttc cttaacctct ttaaccagga ctgcgcgaat gacgaatccg ttactgactc
     6301 cctttgaatt gcctccgttt tctaaaattc tcccggaaca tgtcgttcca gccgtgactc
     6361 aggcgctgaa cgattgccgc gaaaatgtgg cgcgcgtagt agcgcaaggg gcaccgtaca
     6421 cctgggaaaa tctctgccag ccgttggcgg aagtggacga tgtgttgggg cgtatcttct
     6481 ccccggtcag ccacctgaac tcggtgaaaa atagcccgga actgcgtgaa gcctacgagc
     6541 aaaccctgcc gctgctgtcg gaatacagca cctgggtagg gcaacatgaa gggctgtata
     6601 aagcgtaccg cgacctgcgc gatggcgatc attacgccac gctgaacacg gcgcagaaga
     6661 aagcggttga taacgccctg cgtgattttg aactctctgg cataggtctg ccgaaagaga
     6721 aacagcagcg ttacggcgaa attgctaccc gtctttctga actgggcaac cagtacagca
     6781 acaacgtcct cgatgcgacg atgggctgga ccaaactcgt taccgacgaa gcggggctgg
     6841 cggggatgcc agaaagcgcg ctggctgcgg caaaagccca ggccgaagcg aaagagctgg
     6901 aaggctacct gctgacgctg gatatcccaa gctacctgcc ggtaatgacc tactgcgaca
     6961 accaggctct gcgcgaagag atgtatcgcg cttacagcac ccgcgcctcc gatcaaggcc
     7021 cgaacgccgg taagtgggat aacagcaagg tgatggaaga gatcctcgcg ctgcgtcacg
     7081 aactggcgca actgctgggc tttgaaaact acgcctttaa atcccttgcc actaaaatgg
     7141 cagaaaaccc gcagcaggtg ctggatttct taaccgatct ggcaaaacgc gcgcgtccgc
     7201 aaggcgaaaa agagctggag caactgcgtg ccttcgctaa agccgaattt ggtgtcgatg
     7261 agttgcagcc gtgggatatc gcttactaca gcgaaaaaca gaaacagcac ctctacagca
     7321 tcagcgacga gcagctgcgt ccgtacttcc cggaaaacaa atcggttaac ggcctgtttg
     7381 aagtggtgaa acgtatttac ggcatcaccg ctaaagagcg taaagaggtt gatgtctggc
     7441 atccggatgt acgtttcttc gaactgtatg acgaaaataa cgaactgcgc ggcagcttct
     7501 acctcgatct gtatgcccgt gaaaacaagc gcggcggggc gtggatggat gactgcgtag
     7561 gccagatgcg taaagctgat ggttctctgc aaaaaccggt cgcgtatttg acttgtaact
     7621 tcaaccgccc ggtaaatggt aaaccggcgc tgttcactca cgacgaagtg atcaccctgt
     7681 tccacgagtt cggtcacggc ctgcaccata tgctgacccg catcgaaacc gctggtgttt
     7741 ccggtatcag cggtgtgccg tgggatgcgg tcgaactgcc gagtcagttt atggaaaact
     7801 ggtgctggga gccggaggcg ctggcgttta tctctggtca ctatgaaacc ggcgaaccgc
     7861 tgccgaaaga gttgctggat aaaatgctgg cggcgaagaa ctaccaggcg gcgctgttta
     7921 ttctgcgtca gctggagttc ggcctgtttg atttccgcct tcatgccgag ttccgcccgg
     7981 atcagggggc aaaaatcctc gaaactctgg cagaaatcaa gaaactggtt gccgtggtgc
     8041 catctccgtc ctggggccgt ttcccgcacg ctttcagcca tattttcgcc ggtggttatg
     8101 cggcaggtta ctacagctac ctgtgggctg acgtactggc ggcagatgct ttctcgcgct
     8161 ttgaggaaga gggcattttc aaccgtgaaa ccgggcagtc gttcctcgac aacattctga
     8221 gccgtggcgg ttcagaagag ccgatggatc tgttcaaacg cttccgtggt cgtgaaccgc
     8281 agctggatgc gatgctggag cattacggca ttaagggctg atcattcagt gaaaatctgc
     8341 ttaattgatg aaacaggcgc cggagacggc gccttatctg ttctggcggc ccgctggggg
     8401 ctggagcacg atgaagacaa cctgatggcg ctggtgttaa cgccggaaca tctggaattg
     8461 cgcaagcgtg atgagccaaa acttggcggc atctttgttg attttgttgg cggagcgatg
     8521 gcgcaccgac gcaaattcgg cggtggtcgc ggtgaggcgg tggcgaaagc ggtgggcatt
     8581 aaaggcgatt atttgccgga tgtggtggat gccactgcag gactggggcg cgatgccttt
     8641 gtgctggctt cagtcggttg ccgcgtgcgg atgctggagc gtaatccagt tgttgccgcg
     8701 ctgctcgacg acggcctggc gcgtggttat gcggatgcgg aaatcggcgg ctggttgcag
     8761 gagcgtttgc agttaattca cgcctccagc ctgacggcgc taaccgatat taccccacgc
     8821 ccgcaggtgg tctatctcga cccgatgttc ccgcataagc agaaaagcgc gctggtgaaa
     8881 aaagagatgc gtgtctttca gtcgctggtg gggccggatc ttgatgccga tggattgctg
     8941 gagcctgcac gcttgctggc gaccaaacgc gtggtagtaa agcgcccgga ctacgcgcca
     9001 ccgctagcga atgtcgccac gccaaacgcg gttgtgacta aagggcatcg ctttgatatc
     9061 tatgcaggta cgcccgtgta aaagtaaagc agggtagcga taacgctacc ctgctttctg
     9121 caagaataat taatggcttt ccggcgtcgc aatcatgcgt ttcagccacg gcaccatcaa
     9181 cagcatcact actgcaacgc ccagcgtaac caggccaatc ttaccaaaca cgttggtata
     9241 gacgggtaac gtctcaagcg gatcggtaat gttgtccggc actgcggtaa atgttgccac
     9301 atagccgccc agcaagaatg cggcagcctg cgtcaggaac cacatcccga gaataaagcc
     9361 catcaaatgc tgcggcacca gggcagcaat catcgccagg ccaagggcgc taataaacaa
     9421 ttcacccagg ctctggaata aatacaccag cacgataaac catggcgatg tcagcccttg
     9481 tgcatccgca aaccacattc ccgcagctgc cgccgtcaaa aagcccagtg agcacataaa
     9541 catgccgaga gtaaatttca tcggcatcga gaggtctttg cctttgttac ccagatgcgt
     9601 gtaaatgcct gccagtattg ggctggcgag taccacccag aacgggttaa gcgcctggaa
     9661 gctgaccggg tttatggaaa agccgagaat ttcatgatgc acgttgttga tggcaaagaa
     9721 gttcagcgac gttggcatct gggcgtagag aatgtaaaac accaccgctt cgagcatcag
     9781 gacaaaggcg acaaacattt tattgcgccc ggttttatcc agcttgaatg cctgacgaaa
     9841 gaagatgatg gtgacgacga tggagagaac aatcagcacc agattggcga cttctacgtt
     9901 gtgcatcagc catgcacata cgaagatcat caccacgctg ccaagtaata cgtacaacag
     9961 tttgctgaag ctcatcggct tgaagtcggg ttcagaacca atgtctttca ccattccacg
    10021 acaggcgatg taaaccagta atgcgataat taaccccgcc ccgcacaggt tgtaggtgac
    10081 tgaataaccg tatctatcag cgatcacagg ggccagcgat aacgctatca acgagccgat
    10141 attgatcgac atatagaaca gggtgaatgc gccatcaagc cgcggatctt tcggcggata
    10201 gcacttcgaa agcaagctgg ctgggttagc tttaaacagg ccgttaccga cagcgatagt
    10261 ccccagggcg atgaaaatca ggtcaggctt aagtagcgac atgccggtca tgaagtagcc
    10321 aatcgccagc acaagtgctc cgagaacaat ggtgcgtttg gtccccagca ggtggtcgcc
    10381 gacatagccg ccaatggaaa tgaggccata gaccagcgca gcaaaagcac caaaagtgac
    10441 aaaagcctgc tcttgcgaga atccaagctg tttaacgaag aaaaccgcca gtacgccctg
    10501 cacgccgtag tagccgaatc gctcccataa ctcgacaaaa aagatcatga aaaatgggcg
    10561 aggttgctgc agcatcccca tgggtgttgt tgtattcata tttattaacc ttccaatacc
    10621 atcctgaaaa gtatgaacgc gtaagggata acgcgcgaaa aagtttacat ctgtcagaga
    10681 ttcttaatat tgatgcggtg aatagattaa tacactatta caacagaaaa taaccagctg
    10741 atcacactaa tgatggaaaa tatgatagtg ttaaatgttg tattggcagg agattttgat
    10801 tgcgtacgca gatcgtaggc cagataaggg gtttacgctg catcaggtaa caaggcgcgc
    10861 caaaaagcaa aacgcccgca tatgcgggcg ttcaggaaca cgtcgtgggg ggatgattat
    10921 tcttcttcgt cgcgcagcgg aacaatcagc atatcaacgt gaacggtgtt gatcagctga
    10981 cgtgcggaag acattagttt gctccagaag tcctggtggt gaccacaaac caccagatcc
    11041 atatcgtatt tcttgattgc atcgaccaga acctggccca ggtcgccgct gccgctcagg
    11101 gtttcagtga ttgggtagcc tgcattagtg gaaagctcgg tcagcgcatg atgtgtctct
    11161 tcagagatgc gtttctgcat atcacccaga ttcacatcaa taagcccggt gtataggtca
    11221 gagtagttta catctacgtg gatcagagaa actttcgcat tgtaggggcg agccatagag
    11281 actgctttct ctaccagaac tttgctttcc ggggagagat cgaccgcgat gagaatgtgt
    11341 ttataagcca tagtgttact ccttccataa agttgtcgat gactggccag ctagcgtttc
    11401 ttgtgccgtt acgtcacccg cgtcctgcga actacgtgcg cggggctaac ccagccgtaa
    11461 agattcagtg ttaagaccat ccttacctta tagcgacccg gatgatccgt caatccgcct
    11521 tgcttacccg ttagataaac aaattttcga catgaatata ttgatagtgg ttaaccttct
    11581 ggaaaaaaaa caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca
    11641 caaaagcggt ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc
    11701 cccggggtgg aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat
    11761 tattttgggc tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac
    11821 gcgcgttgtt agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag
    11881 ggggcttttt tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct
    11941 atgcccaacg ttatcgcgat catcacgatg atgaatttat tcgccgctgt gagcgggtgc
    12001 gtcggcagtt tattctgacc agcgcattgt gtggtctggt ggtggtcagc ctgattgcat
    12061 tgatgatttg gcattaagca cttcagcaat aataacgcat aaaaaaagcg ggccagtaaa
    12121 ctgacccgct cgagactgat tacaaacgca aaattgcctg atgcgctacg cttatcaggc
    12181 ctacataatc tctgcaatct attgaatttg catgtttttg taggccggat aaggcgttca
    12241 cgccgcatcc ggcatgaaca aagcgcacta tgtcacaatc tgaagcgggc cagctgaccc
    12301 gctcgttttg gtgcgtacga ttacaggaac tgcaaggaga gccagtacag cccgccggaa
    12361 agcagtaccg cagccggaag ggtaaacacc caggccatca gaatactggt tacggtttta
    12421 cgctgtaagc cgccgccatc taccaccatc gtccccgcga cagaagagga gagtacgtga
    12481 gtagtggaaa ccggcatccc ggtataactc gccaggccga tagacactgc cgccgtcatc
    12541 tgggcagaca tcccctgagc gtaggtcatg cctttcttac cgattttctc accgatagtc
    12601 gttgccacac ggcgccagcc aatcatcgta ccgataccta acgccagcgc gaccgccatg
    12661 atgatccaca ccggtgcata ctcgatggtg ctaagcatgt cggacttcag tttcttcaac
    12721 aggcgctgat cgtcagcact cacgccaggc atcttcacca ctttgtcgat agtgtcagaa
    12781 acgcacagca taatgcggcg catctggcta cgttgatcaa gcgacagctt gtcgtagctt
    12841 tccacatcgg tggtcaacat acctttcagg cggttgagcg cgttaatggt attcgacgga
    12901 tggcagtgga actccgcagg ttgcgttgcg ccagcttccg gagccggtac taactgatca
    12961 gcaccggtag cctgtttgag cagcgcagga tgctgctcaa agtaagcttc gacgttgttg
    13021 atggcatcac gggtacgggt gatttcgtag ccagtggcat tcatgttcac cacgaagcct
    13081 gctggcgcga cgccaatcaa taccaacata accagaccaa tgcctttctg accatcgttc
    13141 gcgccgtgcg aaaacgccac gccgatagcg gaaaggatca gcgcgatacg tgtccagaac
    13201 ggcggctttt tcttgccgtc tttcttttca cgctccgctg gggtcaggtg gatacgggcg
    13261 cgtttcttgg tgccgctcca gtagcgacgc agcaagaaaa tcagaccgcc agcaaacacc
    13321 aggccgacaa taggggaaac gatcagagaa ccgaaaatac ttaatacttt cgggatattg
    13381 agtgcatcca ccactgacgt cccggtcatc aacgcattgg ttaaaccaat cccgatgatc
    13441 gcgccaatca gcgtatgaga gctggatgca ggtaaaccaa agtaccaggt acccaggttc
    13501 cagataatcg ccgccagcaa catagagaac accatggcaa ggccatgaga cgatcccata
    13561 ttaagcagca gatccgtcgg cagcatatgc acaatggcat aggcaacact cagaccaccc
    13621 agcaaaacac ccaaaaagtt gaacaccgcc gccataacca cggcgagctg agaacgcatc
    13681 gcgcgggtat agataacggt tgccacggcg ttggctgtgt catggaaacc attgatggct
    13741 tcgtagaaca gcacaaaagc cagtgcaagc aataataaca gcccggtatg caaatccagg
    13801 ccagcaaaca aatgtagcat aggacgttac gccattttga ggacatgaac gcggcgcatt
    13861 atcagtgact ttcgcggcgc gggcaaagtg aaatatagac tttttttgat ttgcctcctg
    13921 tatggatttc actcaaaaaa taattatctt atataattca ggcaaatact tccttttagt
    13981 aatattgatg ctggtgcgac cactgaggaa tctttacaat tcacgcccgt tttttctaag
    14041 aggagcgcaa cgtggaaagg tttgatgcca ttattatagg cgctggtgcg gcgggtatgt
    14101 tctgttctgc gctggcaggt caggcaggac gccgggttct gctgatcgat aatggtaaaa
    14161 aaccagggcg caaaatcctt atgtctggcg gtgggcgctg caactttacc aacctttatg
    14221 tcgaaccagg cgcttatctg agccagaatc cgcatttttg taagtctgca ctcgcacgtt
    14281 ttacccagtg ggatttcatt gatctggtca ataaacacgg catcgcatgg cacgagaaaa
    14341 cgttagggca actcttctgc gatgactccg cgcagcagat tgtcgacatg ctggtggatg
    14401 agtgcgagaa gggcaatgtg accttcagat tgcgtagcga agtgctgagt gtggcggagg
    14461 atgaaacagg cttcacgctt gatctgaacg gcatgactgt cggttgcgaa aagctggtca
    14521 tggcgaccgg tgggctgtca atgccggggc tgggcgcgtc gccgtttggt tataagattg
    14581 ccgaacaatt tggcctcaac gtgctgccga cccgtgcggg tctggtgcca ttcactctgc
    14641 ataaaccgtt gctcgaagag ttacaggtgc tggcgggcgt ggcggtgcct tccgtgatta
    14701 ccgctgaaaa cggcatcgtt ttccgtgaga acttactctt cacccatcgc ggcttgtctg
    14761 gaccggcggt gttgcagatt tccagctact ggcaaccggg tgaatttgtc agtatcaatc
    14821 tgctaccgga tgtggatctc gaaaccttcc tgaatgagca gcgtaacgca catccgaatc
    14881 agagcctgaa aaacacactg gcggttcatc taccgaagcg gttggttgaa cgcttacagc
    14941 aactcgggca aatcccgaat gtttcgctaa aacagctcaa cgtgcgtgac caacaggcac
    15001 tgattagcac attgaccgac tggcgtgtac aacccaacgg cactgaaggc tatcgcactg
    15061 ccgaagtgac gctcggcggc gtggacacca acgaactctc ttcacggacg atggaagcgc
    15121 gcaaagtgcc tgggctgtac ttcatcggcg aagtgatgga cgttaccggc tggctggggg
    15181 gctataactt ccagtgggcg tggtcgagtg cgtgggcttg tgcgcaggat ttgattgcag
    15241 cgaagtcgtc ctgacctgtt acctaaagat gaaaattatt agagccgcat taaaaatgag
    15301 ctaattttga tagtggctat cttgtgatta ttttctaatg agcccgtgaa ctgaaaccct
    15361 ccaggcttaa tataaggtgg aaggaaaggt gattgaaaac tcacccagtg gccagatctt
    15421 tataaaatat gaacaggctg aaaaagacag taactttgtt gtttttttat ccttaaccac
    15481 tatgcattaa tgctgcgtta tttcatgatg cctaagaaaa accagcgtta cgcaaatggt
    15541 caacgctggt tttatccggt acgttgcaat tattttttag cagaacccgc ttctaatatt
    15601 gaaacgattg agaacaacgt aaagcatacc gctcccaaac cgaccagaac tcgcgaagga
    15661 ataaaatatc ccatgtcggt ctgcgccatt tccgcaagaa atgatgccag gaaaaggcaa
    15721 aacaggcagg tgaagacggg tatcatcggt attctgttgg ctaacgaaca ggtacgtctc
    15781 cagaccagtg ccagtagcca gacttttgag aatatgctgt aacagatcat tccgaggcaa
    15841 ataagaataa tgccgggagc cagtcgggcg cttgcatcgg aactgactaa gacgtatatc
    15901 ccctgcagta ccgtgattga gccgagaaaa ataacccaat aacaccacag ccagtgttct
    15961 ttagttgaaa acgtattacg tgtttgatgg acaattgtgg caacaaggcc aattagacag
    16021 gcacagattg cggttaaccc aagcaatacg tgacccgcga cataatgcgg agttatgtca
    16081 gcactacgta acagcgtaat agaccaaatg aaaccgagca gggtgagtaa aacgggaacg
    16141 gcaatgaggc aattaccgat taatgaagaa taagcctgta ccggcgttcc atcgctcttg
    16201 ctccccgctg catttttggg aatgagcaga aagtgaccgg atgacgctgc caccgtcgat
    16261 acacaggcgg caatcatacc aacgccgaaa ataacatggc cggcgacaaa ctcgtctgcc
    16321 atcacatcat tgcctgctaa cagtgcccaa ccccatatca tggtgataat tgaccccgca
    16381 tagccaataa tggggaacaa tgtattgtaa aacgtattaa cgccgcgcgt gagctgcgaa
    16441 atgataataa atgcggtagt gaataatgcc aggcatatgg ccgccagaga aatcagcaca
    16501 tgacccgcga cgaaatactc gctttgccct gaactttcca gcacaaagcc acccgatata
    16561 tatgttcact ttaaactcct caggtatccc tgctaataat gaaatgtgca ttaatgataa
    16621 tttcagcctg ttaatataac gacgagatat cattaaagac aaatgcatag cataactata
    16681 gcggggttta ctgtgccgag tgccattttt taccggttaa aaggccatga taaataaata
    16741 tttatcctaa gattaattct tattctgttg ttttttttac aattatcttc cagggatgga
    16801 ttgttttcca ttatttagta aaagaccgga tagtctcggt gcttaaaatc atcgaacata
    16861 taagtattat tgctttgtat catcgtaaat atgtctttca gacatgtatt gtttattttg
    16921 ttactaacaa gtaaaaatat tacaggttat ttatgaaagt atttcacaat atatttaagc
    16981 acttctcttc aaaccatcaa gataagcatt cagataaagt taacagtcat caacaccatg
    17041 gcaaggttga taaaaaatat cgatcaaaca cagttgaagt taatatgctg aatgactttt
    17101 cgcaaataga taacgattta ggcctgaata tcatctattt cctgcaacat ggtcactgga
    17161 gggtaaatga acatagtctt cagatggaga aaatatggtt ttacaatagt gagccatcta
    17221 tagatattca agaatataat agtttcgccg ataatactac taacacgttt atctttacaa
    17281 ttataccaga caataaccat gtgataaaat tatcgtctcc cattacagtt aagattgagt
    17341 gtaaggatgg ttattatttt attaatttct ccggagataa atcagacata atttataaat
    17401 cagacataac ttataaagtt aatgggcttt ctatcatacc tgaaaatttc tttactcttc
    17461 ttagcggtaa ttttaaagct gactggcgtt gggatgtctc taaagagacg tttcctaagg
    17521 ataagtttga tagctatgtt aaatctgtgt tttctaaaat agacttttat aaacaatgtg
    17581 gtgtgattaa cccacaaaat gccaacacgg cttattttgg cgatacagat ggaagagtag
    17641 gcgctgtact ttatgcattg ctcgtttcag ggcatatagg catcagggaa aagggttgga
    17701 gtttattatg tgaattatta aaacacgaaa acatggcttc atctgcatac atacataaaa
    17761 ataataaatt gctctacaat ttattaaata cgagagacat gatattgaat gagttgcatc
    17821 aacatgtttt tttaaaggac gatgcgataa ccccttgtat cttccttggg gatcatacag
    17881 gcgatagatt tagtactatt tttggtgata aatatatcct tactttgcta aattctatgc
    17941 gaaatatgga tggtaataaa gatagccgta ttaataaaaa tgtcatcgtt ctggctggaa
    18001 atcatgaaat caattttaat ggtaactata ctgcacgact agaaaattat aaactctctg
    18061 ccggtgatac gtataactta attaaaacgc tcgatgtttg tcattatgac tcagaaacga
    18121 aagttttaac cagtcatcac ggaataataa gaaatgaaga aaatgaatgt tattgtctcg
    18181 gtgctttgca ggttccattt aatcagatga aagacccaat tgatcctgag gaattagcaa
    18241 atatatttaa taaaaaacat aaacaacaca tggatgatcg tcttatccat ctaattagaa
    18301 gcaatgctgt agcatccaca cctgtatatg aaaattattt taataacact actgctttca
    18361 gaccaaaacc tgaagatatc ttcaaatgtg gacagacgct caaaaaaact aaacagaaat
    18421 atggccatta tggattaggg gtagatcagc atcaaaaaat tgataattat accatggggc
    18481 taaattcctg gaagatagca cctaacgaac gtggtgataa aaaaggtgtc ccagggctta
    18541 gttgttttca acctcaataa aacttcatga gtgctgtggt atctcttttt aattttgcca
    18601 gtatttcata ggttttctta atcgattgta gaggcatgtt ctgatgacgc ctctacaatc
    18661 tttagggact ttctctgcta tgagataaga aatttcatat tatgctttaa ggctgaatga
    18721 tttgtgcaaa atataattat tttcccatcg aacatgaggt gtatgtcata agattaaatg
    18781 ttaactatgt attcctcgcg gtaaataata tgaggtaaaa gatgaaaatt ggaactgtgg
    18841 caggcactaa cgacagtaca acgacaatca caacaaatga tatggtgcaa gagcatgtta
    18901 ccaacttcac taaagaactg tttgggtata ttgcaaatgg tataggtgat gatatatcaa
    18961 gtattgccag gacaatgctc ggtgaggttg tggaaaaaat agatgattgg caaatagaac
    19021 ggttccaaca atctattcaa gatgacaaaa tttcatttac catacaaacg aatcattcag
    19081 aaaaatatag tatgctatcg ggcatgcgcg ctcatattct acgtaggaat aactgttatc
    19141 agtttattgt gacaattaat agtaaaaatt acggttgccc tcttgataat accgatatta
    19201 attggtgcag cattgtttat ttacttaata atatgacagt aaatgataat gctaatgatg
    19261 ttgcagttac tgagtcgtac aaacccatct ggaactggaa aatatcgcaa tacaatgtat
    19321 ttgatattaa attcgaaaca atgataaaac cccagttcgc tgacagaacc tatttctcca
    19381 attgttcacc tgttgatccc acaagtacca gaccgaccta ttttggcgat accgatggct
    19441 cagtcggtgc agttttgtac gcgctttttg caacgggaca tttgggaatc atggctgaag
    19501 gagaaaattt ccttagccag ttgcttaata tagaagatga agttctcaac gtattgctaa
    19561 gggagaactt taatgagcaa cttgacacta atgttaatat aataatcagt atcctgaaca
    19621 gaagagacat tgttttagaa agtcttcagc cttatttggt tattaataaa gacgcagtca
    19681 cgccatgtac gtttctgggc gaccagactg gcgatcgatt tagtaatatc tgtggcgata
    19741 aatttatcat cgatttatta aaacgcataa tgagtatcaa tgaaaacgtt catgtattag
    19801 ctggaaatca tgaaacgaat tgcaatggta actatatgca gaacttcacg cgcatgaaac
    19861 cacttgatga ggatacctac tctgggataa aagattatcc tgtatgtttc tatgacccca
    19921 aatacaaaat aatggcaaat catcatggga taacttttga tgatcagcga aaacgttaca
    19981 ttataggacc gataacagtc tctagtgatg aaatgaccaa tgctctcgat ccggtcgaat
    20041 tagcggcaat tatcaataaa aagcaccatg ctgtaattaa tggcaaaaag tttaaaaata
    20101 gccgggctat ctcttgtcga tcctttaatc gttatttttc tgtttcgaca gattacagac
    20161 ctaaacttga agcactatta gcatgctcac aaatgttagg tataaatcaa gttgttgcac
    20221 ataacggtaa tggtggaaga gaatgtatag gtgaaacggg gacagtttta ggtcttaacg
    20281 cccgtgacag taaacatgct ggaagaatgt tcagcatgca taattgccaa attaaccctg
    20341 gtgcagggcc tgagataacc actccctgga agtcttacca gcatgaaaaa aacagaaatg
    20401 gacttatgcc tttgatcaga aggcgcacaa tgttacagct ctgaaccata gacaacatga
    20461 atattaatgt tactatctgg ttgagactgt tgataaaacg taaaaaggaa tgctctgttc
    20521 ctgcaactga acaggcgtga aacgctatcc aacaggatgg ataccgtttt gtcagccagt
    20581 tttatagatc acacttatga gtatgcgtgt ttcatttacg gccaggaatg ctgatgatga
    20641 caacattact acccgttttc actaagcctt ccccgttggc gctcaatgct ctgcgcgctg
    20701 gtcgtatttg tcgtttcctt cttatcccgg atggaagaat ccgctgaacg tcgttcggcg
    20761 gcagttgtaa cacgcgtcgt ttgcttgcag ggattatggc gggtcgatta tggataagag
    20821 taagcgccat ctggcgtggt gggttgtcgg gttactggtg gtggctgccg tcgtggcgtg
    20881 gtggctgttg cgcccggcgg gtgtgccgga aggctttgct gtcagcaatg ggcgcattga
    20941 agcgacggaa gtggatatcg ccagcaaaat tgccgggcgt atcgacacca ttctggtgaa
    21001 agaaggccag tttgttcgcg aaggcgaagt gctggcgaag atggatactc gcgtgttgca
    21061 ggagcagcga ctggaagcca tcgcgcaaat caaagaagca caaagtgccg ttgctgccgc
    21121 gcaggctttg ctggagcaac ggcaaagcga aactcgtgcc gcgcagtcgc tggttaatca
    21181 acgccagtcc gaactggact ccgtggctaa acgtcatacg cgttcccgct cgctcgccca
    21241 acgaggggct atttctgcgc aacagctgga tgacgatcgc gccgccgctg agagcgcccg
    21301 agctgcgctg gaatcggcaa aagctcaggt atcggcttct aaagcggcta tagaagcggc
    21361 ccgtaccaat atcattcagg cccaaactcg cgtcgaagcc gcgcaagcca ctgaacggcg
    21421 tattgccgca gatatcgatg acagtgaact gaaagccccg cgtgacggac gcgtgcagta
    21481 tcgggttgct gagccagggg aagtgctggc ggcaggcggt cgggtgctga atatggtcga
    21541 tctcagcgac gtctatatga ctttcttcct gccaaccgaa caggcgggca cgctgaaact
    21601 tggcggtgaa gcccggctga tcctcgatgc cgcgccagat ctgcgtattc cggcgaccat
    21661 cagttttgtc gccagtgtcg cccagttcac gccaaaaacc gtcgaaacca gcgatgaacg
    21721 gctgaaactg atgttccgcg tcaaagcgcg tatcccaccg gaattactcc agcagcatct
    21781 ggaatatgtc aaaaccggtt taccgggcgt agcgtgggtg cgggtgaatg aagaactttc
    21841 gtggcctgac gacctcgtgg tgaggttgcc gcaatgacgc atctggaact ggttcccgtc
    21901 ccgcctgtcg cgcaactggc gggcgtgagc cagcattatg gaaaaaacgt tgcgctgaac
    21961 aatattactc tcgatattcc ggcccgctgt atggtcgggc tgattggtcc ggatggcgtc
    22021 gggaagtcga gcttattgtc gttgatttcc ggtgcccgcg tcattgagca gggcaatgtg
    22081 atggtgctgg gcggcgatat gcgcgacccg aggcatcgcc gcgacgtctg cccgcgcatc
    22141 gcctggatgc cgcaggggct gggcaaaaac ctctaccaca ccttgtcggt gtatgaaaac
    22201 gtcgattttt tcgctcgcct gttcggtcac gacaaagcgg agcgggaagt gcgaattaat
    22261 gagctgctga ccagcacggg gttagcaccg tttcgcgatc gtccggcagg gaaactctcc
    22321 ggcgggatga agcaaaaact tgggctgtgc tgcgcgttaa tccacgaccc ggaactgttg
    22381 atccttgatg agccaacaac gggggttgac ccgctctccc gctcccagtt ctgggatctg
    22441 atcgacagta ttcgccagcg gcagagcaat atgagcgtgc tggtcgccac cgcctatatg
    22501 gaagaggccg aacgcttcga ctggctggta gcgatgaatg ccggagaagt gctggcaact
    22561 ggcagcgccg aagagctacg gcagcaaacg caaagcgcta cgctggaaga agcatttata
    22621 aatctgttac cgcaagcgca acgccaggcg catcaggcgg tagtgatccc accgtatcaa
    22681 cctgaaaacg cagagattgc catcgaagcg cgcgatctga ccatgcgttt tggttccttc
    22741 gttgccgttg atcacgttaa tttccgcatt ccacgcgggg agatttttgg ttttcttggt
    22801 tcgaacggct gcggtaaatc caccaccatg aaaatgctca ccggactgct gcccgccagc
    22861 gaaggtgagg cgtggctgtt cgggcaaccg gttgatccaa aagatatcga tacccgccgt
    22921 cgggtgggct atatgtcgca ggcgttttcg ctctataacg aactcaccgt gcggcaaaac
    22981 cttgagttac atgcccgttt gtttcacatc ccggaagcgg aaattcccgc aagagtggct
    23041 gaaatgagcg agcgttttaa gctcaacgac gttgaagatg ttctgccgga gtcattgccg
    23101 ctcggcattc gccagcggct ttcgctggcg gtggcggtga ttcatcgccc ggagatgtta
    23161 atcctcgatg agcctacttc tggtgtcgat ccggtggcga gggatatgtt ctggcagttg
    23221 atggtcgatc tctcgcgcca ggacaaagtg actatcttca tctctaccca ctttatgaac
    23281 gaagcggaac gttgcgaccg catctcactg atgcacgccg gaaaagtgct tgccagcggt
    23341 acaccgcagg aactggttga gaaacgcgga gccgccagtc tggaagaggc atttatcgcc
    23401 tatttgcagg aagcggcagg gcagagcaac gaagccgaag cgccgcccgt ggtacacgac
    23461 accacccacg cgccgcgtca gggatttagc ctgcgccgtc tgtttagcta cagccgccgc
    23521 gaagcgctgg aactgcgacg cgatccagta cgttcgacgc tggcgctgat gggaacggtg
    23581 atcctgatgc tgataatggg ttacggcatc agtatggatg tggaaaacct gcgctttgcg
    23641 gtgctcgacc gcgaccagac cgtcagtagc caggcgtgga cactcaacct ctccggttcc
    23701 cgttacttta tcgaacagcc gccgctcact agttatgacg agcttgatcg tcggatgcgt
    23761 gcgggcgata tcacggtggc gattgagatc ccgcccaatt tcgggcgcga tatcgcgcgt
    23821 ggtacgcctg tggaactcgg cgtctggatc gacggagcga tgcccagtcg tgccgaaacg
    23881 gtaaaaggtt acgtgcaggc aatgcaccag agttggcttc aggatgtggc gagccgacaa
    23941 tcgacacccg ccagccaaag cgggctgatg aacattgaga cgcgctatcg ctataacccg
    24001 gacgtaaaaa gcctgccagc gattgttccg gcggtgatcc cgcttctgct gatgatgatc
    24061 ccgtcaatgc taagcgccct tagcgtggtg cgggaaaaag agctagggtc gattatcaac
    24121 ctttacgtga cccccaccac ccgtagcgaa tttttgcttg gcaaacagct gccgtacatc
    24181 gcgctgggga tgctgaactt tttcctgctc tgcggcctgt cggtgtttgt gtttggcgta
    24241 ccgcataaag gcagtttcct gacgctcacc ctggcggcgc tgctgtatat catcattgcc
    24301 accggaatgg ggctgctgat ctccaccttt atgaaaagcc agattgccgc cattttcgga
    24361 acggcgatta tcacgttgat cccggcgaca cagttttccg ggatgatcga tccggtagct
    24421 tcgctggaag ggcctggacg ttggatcggc gaggtttacc cgaccagtca ttttctgact
    24481 atcgcccgcg gaacgttctc gaaagcgctg gatctgactg atttgtggca actttttatc
    24541 ccgttgctga tagccatccc gctggtgatg ggcttaagca tcctgctgct gaaaaaacag
    24601 gagggatgat gcgccattta cgcaatattt ttaatctggg tatcaaagag ttgcgcagtc
    24661 tgctcggtga taaagcgatg ctgacgctga ttgtcttctc gtttacggtg tcggtgtatt
    24721 cgtcagcgac cgttacgcca ggatcgttga acctcgcgcc gatcgccatt gccgatatgg
    24781 atcaatcgca gttatcgaac cggatcgtta acagcttcta tcgtccgtgg tttttgccac
    24841 cggagatgat caccgccgat gagatggatg ccggactgga cgccggacgc tataccttcg
    24901 cgataaatat tccgcctaat tttcagcgtg atgtcctcgc cggacgccag ccggatattc
    24961 aggtgaacgt cgatgccacg cgcatgagcc aggcatttac cggcaatggg tatatccaga
    25021 atattatcaa cggtgaagtg aacagctttg tcgcgcgcta ccgtgataac agcgaaccgt
    25081 tggtatcgct ggaaacccgg atgcgcttta acccgaacct cgatcccgcg tggtttggcg
    25141 gggtgatggc gatcatcaac aacattacca tgctggcgat tgtattgacc ggatcggcgc
    25201 tgatccgcga gcgtgaacac ggcacggtgg aacacttact ggtgatgccg ataacgccgt
    25261 ttgagatcat gatggcgaag atctggtcga tggggctggt ggtgctggtg gtatcgggat
    25321 tatcgctggt gctgatggcg aaaggtgtac tgggcgtacc gattgaaggc tcgatcccgc
    25381 tgtttatgct gggcgtggcg ctcagtctgt ttgccaccac gtcaatcggc atttttatgg
    25441 ggacgatagc gcgttcaatg ccgcaactgg ggctgctggt gattctggtg ctgctgccgc
    25501 tgcaaatgct ttccggtgga tccacgccgc gcgaaagtat gccgcagatg gtgcaggaca
    25561 ttatgctgac catgccgacg acacactttg ttagcctcgc gcaggccatt ctctaccggg
    25621 gtgccggatt cgaaatcgtc tggccgcagt tcctgacgct gatggcaatt ggcggcgcgt
    25681 ttttcaccat tgcgctgctg cgattcagga agacgattgg gacaatggcg taaggaaggg
    25741 aaacttgact tcatactgtg atgtcatact attgatgtca taaagtgaag tcataaggac
    25801 atgccatgag aaccaaggta caggctttac ggaagaaaaa aaaatacatt ggatcaaata
    25861 tttaaaactc ctgttcctca agggatcaag tggtctgata tagagtcact ggttaaagca
    25921 ttaggcggag aaattaagga aggaagaggt tcgcgttgta agttcatact aaatatgagc
    25981 gttgcgtgtt tccatcggcc tcatccgtcg ccagataccg ataaaggcgc tgtagaaagc
    26041 gtgcgtgact ggttactaag tataggggta aaaccatgat caaactgaag acgccaaact
    26101 ctatggagat tgccgggcaa ccagctgtta ttacctacgt gccagaactg aatgcatttc
    26161 gtggcaaatt cctgggcttg tcaggttatt gtgattttgt ctctgacagc attcaggggc
    26221 tgcaaaaaga aggggaactt tccctgcgag agtatcttga ggattgtaaa gctgcgggta
    26281 ttgagccgta tgcgcgtaca gaaaagatta agacctttac gttacgctat ccagaatcgc
    26341 tgagtgaacg ccttaataat gcggctgcgc agcaacaagt ctctgttaat acctatatta
    26401 ttgaaacact gaatgagcgt ttgaatcatt tataaatttt agcggcgtac attacgcaga
    26461 atatctgcct ttacaggatc tatctatgca actgaactta attttataaa ttaagttcag
    26521 ttaatatctt ttctttcctc gtttcagaaa taactcaatc ttccttcggc aagcactgca
    26581 aatgcccatg ccgcacgccg cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc
    26641 ccatatcacc tttcagcacg gcgatttcca gacagtcgtc gtgattgata tgcacatgca
    26701 gcgtggcgac ggagagatcg tggtgatggt gctgggtgga gacaatgcgg ctggctaagt
    26761 cgcgtttttc gtgttcatac acataggaga gcaccgcgaa accttgcgtg ccgtgctgct
    26821 gagtggcctc ctgggccagg gcgctacgca gaatatcgcg gatagcttcg gaacggttgt
    26881 tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca tcaagcgtga
    26941 tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca cggggaatgc
    27001 gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc
    27061 caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct gacagaagcg
    27121 ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact gttgttgtag
    27181 ctttttcagc aggcgaatga cgcccgcctg taacacgaga tcaaggttag aaacggcttc
    27241 atccagaatc agtagtttcg gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag
    27301 ctggccgccg cttaactgcg gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc
    27361 cttcagcatt tcgctggcgc gcgccagttg ttcggatttt ttcagtgata gcaggtggcg
    27421 catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc
    27481 ctgaaatacc atctgaatat cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc
    27541 cagcggttcg ccacgccagc taatattccc ctgcgcgggc gattctaaac ccaccagcaa
    27601 ccgcgcgagg gtacttttcc cgcagccgct gcgccccagc agggcgacgg tttcgccgct
    27661 tttcagggtc agggaaacgt tattcagcac cgcctgatgt tgatgttttc cgttaaatcc
    27721 accgtgggca tagtgatggg aaaggccgga gacgttaagt aaagtcatga tgccagctcc
    27781 ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg tttgggggcg
    27841 ttaaacagcg tttctacatc gccctgttcg acaattttac cttgtgacat caccgccacg
    27901 tcatccgcca gacgcgccac aacgcccata tcatgggtga ccagcagcat tcccggcgct
    27961 tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg
    28021 tcggtggtcg gttcatcggc gatgataaac ggtgattcac acagcaccgc catcgcaatc
    28081 atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg
    28141 gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc
    28201 cctaacgcca ggcaggtttc ccgcgcgtgg gtgtgcatgg tgtgtagcgg attaaaggcg
    28261 ctgcgcgggt tctgcatgat ggtggcaatt ttgatgccgc gcagggcgca gggataaacc
    28321 gctttgccat cggctaaaat ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc
    28381 agcgtcgcgg cgcaggttaa tgatttcccg ctgccgctac cgccgactaa cgccagcacg
    28441 cgcccgcgtt gcagggttaa cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg
    28501 atattacgta gttcaatctg ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat
    28561 ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg ataaacagcg
    28621 ccagccccgg ccagaacatt tgcagcggct gggtccagat atactggcgc gcgtcgttaa
    28681 tcatcacgcc ccattcggcg gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg
    28741 cgacgtgcag catcatatgg ccgatatcca gcgttgccag caccagcagc gaagggatca
    28801 ccgcgcctgc cagatgatcg acaaacaccc gcacatggcc cgcaccggaa agccgtgacg
    28861 ccagcacaaa ctcgcgttgg cgtagtgaaa tcaccaagct gcgcaccatg cgtgcatacc
    28921 acgcccagtg cgacagggcg atggcgataa ttacgttggt cagcccggtg ccgagcacgc
    28981 caaccataaa gaacgacaga atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca
    29041 tggtggcctg atcaacgcgc ccgccaatca acccggcgct gccgccaata accagcccta
    29101 atgtcagcac cagcagcagg caggccatta ccgaaccgag cgacacgcgg gtcgctgcca
    29161 tcagccgcga gaaaatatcg cgacctaagt gatcggtgcc cagccagtgc tgcgcatccg
    29221 gcgaaagcag gcgcgacggc aaatcaatcg cctgggggtc atacggcagc caccactggc
    29281 tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcgt accgaccagc
    29341 gggaagagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag
    29401 cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca ccatcatcag
    29461 cgtaaagcac tgaatcaccg gatagtcacg gttaaaaatc gccgacaccg catagcgccc
    29521 gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca gttcgccgat
    29581 gtgcatcccc acggcggtga tcatcggcag cgaggcattg cgcagaatat ggcggcgttc
    29641 ggtctgtttg tcgttcaggc cgcgcagacg cgcccaagtg acgtgacgct gaccggcgac
    29701 gtccagcata ctggcgcgca gcagtcgcgc gttaatcgcc agcgacataa aggcaatgga
    29761 aaccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta gccattgcag
    29821 atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca tcgacacgcc
    29881 aagaaacgcg atgaaacgta cggcgaaatc cggcagacgg tcgcgatggc gcgccgccca
    29941 gataccgagc ggtacggaag tgagcagaat taataccagc gccgcacctg caagttccag
    30001 cgtggcgggc aggaagttca gcatatcgtc cagcaccggg cgttggctgg cgaatgagat
    30061 accaaagtca agatgcagcg ccttccacaa ccaggtaccg tactggacgt acagcggctg
    30121 atccagcccg agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga
    30181 taaacgcaga taatcgagcg ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat
    30241 caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa
    30301 cattaaggtt tcaccggttt aatctgttcg aacggaattt cggtggcgat cggcgcgtag
    30361 gggatattac ccagctccgg ttttgatacc accatcatcg agatgtagct gataggcaga
    30421 taaaccgcct cgtcatgcag acgggtcaaa atgtcgcgat acagcgcctg acgttgcgtt
    30481 tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt gtcggctaat
    30541 ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt
    30601 ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga
    30661 cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc aatctggcgc
    30721 atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc gatgaacgac
    30781 agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcccgc aggcagcgtc
    30841 caaccggctt tttccagcaa ctctttcgct ttctgcggat cgtactggcg cggtttcagg
    30901 ccgagattag cgtaaggcac agaaggggca aacagggtgt cggcgacctg cggggtgcca
    30961 tataacgcgt tatcaatcag cgattttttg tttaccgcgt aattaagagc ttcacgtact
    31021 gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt ttcgatcggc
    31081 tgtgacagtt gggtgtggta agccggattc tggctaaagc gggcgaaggt atcgagcggt
    31141 aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg
    31201 gtagtcgggt ccgggatgac gttaaaggtg atctttttaa tcgctggctt ttcgccccag
    31261 tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg caaaatccac
    31321 ggtccggtgc caatcggcgc tttaattccg ttcatggttt catggttttt aaactgcgag
    31381 ggagcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg atagtaggcg
    31441 cttttcaggg taatttgcag ctctgtttta ctgagtgctt taacatcaac aatctggttt
    31501 gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc
    31561 gccgcctcgg catcgaacgg ttcaccgttg gagaatttca cgtcatcacg cagggtgaag
    31621 gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg gatcaccgaa
    31681 ccgtctgcct gatatttcac caatggttca taaaccatgc tctgggcgaa catctggtta
    31741 ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt agtgatttca
    31801 tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg
    31861 cggagtgtgg agagcatggt aaccccaatg gattaaaata gatggcggaa ataagtatga
    31921 cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg gcagtcgggt
    31981 gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg
    32041 tgggggtgca gatcgcaagg cttaaatttt ctagctgaca atggctgact gacagcgagg
    32101 agtgataggt gctatctacg ctgacgattt gccaggcgct gccgccacgc tgtttaacga
    32161 tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca ggatgcacgg
    32221 cgtccatttc agcgtgttcc ccgaggctga acacggcgtt cgccagccag cgccagttgg
    32281 cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc aacagcagag
    32341 cgatatcgtc accgctatgg cttaagttga accatagcgg cgtttccggc gcgaatgcag
    32401 gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg
    32461 cacgccccgc cagccagcgt tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg
    32521 gtggcagtgg agctgcgctt aaggtcgaaa ctttccccag aactatccga tacatatcag
    32581 ggccaacgtt taatggaaaa tgaaagtgcg tatcgtatca cttgtcgcct catcccggta
    32641 accgactttt cggtctgcct ggccccagta aaatcgccag tttgctaccg cctttggtgg
    32701 tttccatcca gattttacac acactggtta atggcaccga aagtagcatc cctaccgggc
    32761 cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac atccccaggc
    32821 gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc actaaaaaca
    32881 atgcgccgac cagaatacat tcgtaaacac cattaaacag cagcacctga atcattggcg
    32941 gtacggcgga aattaccgcg ccgatattgg gcacgtagtt gagcaaaaac gccagtactg
    33001 cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg atgacacctg
    33061 tccataaact gagtagcgtc ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt
    33121 gtaatcctgc gatgtgaatc tgtgggttat tcagcgcaaa acgcattttg taagggacgt
    33181 ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg ctcgccattg
    33241 ccccggaaag cccggtcatt agcgctgtgg tgaaggtaac cactttttcc gagtccatcc
    33301 gctgcagcat tcgctccggc gacatatgca aattaagaaa aggcaacatc tcctgcaatt
    33361 taaaaagttt gcgcgtcagc tccttattaa acttcggcag catagagata aattcgttaa
    33421 acgatgccgc cagtacgccg accagcgcgg ttagtgcgat cagcatcacc accactacaa
    33481 tcgtaatggc aacggggcgt tgtactcccc gacgaataaa ccaggtaacg agcgggttga
    33541 ggacgatggc aaaaaacagc gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa
    33601 tgcccgcgag gatcactacc agcgaggcca gcttgagcag aatgtgcatg cccgttttat
    33661 cgggttgagg gatttccatt ggggcttcct tgtgactttt tgtattaagt gtagcgggag
    33721 tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg
    33781 aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga acccgcgcta
    33841 aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt cgccagctac
    33901 ctcaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt gatgggcttt
    33961 agcgctttct gggcaggatt ggttatcagc ctgcaatatt tcgccacctt gctgagccgt
    34021 cctcatgccg gacgttacgc cgatttgctg ggccccaaaa agattgtcgt cttcggttta
    34081 tgcggctgct ttttgagcgg tctgggatat ctgacggcag gattaaccgc cagtctgccc
    34141 gtcatcagcc tgttattact ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt
    34201 gccggaacgg gatcgaccct gtggggcgtt ggcgtggttg gctcgctgca tatcgggcga
    34261 gtgatttcgt ggaacggcat tgtcaattac ggggcgatgg cgatgggtgc gccgttaggc
    34321 gtcgtgtttt atcactgggg cggcttgcag gcgttagcgt taatcattat gggcgtggcg
    34381 ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa aggcaaacca
    34441 ctgccgtttc gcgcggtgct tgggcgcgtc tggctgtacg gtatggcact ggcactggct
    34501 tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc taaaggttgg
    34561 gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta
    34621 ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg ctttagcgtt
    34681 gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc gaaagtcggc
    34741 gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattgggcgt agtggcggta
    34801 aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt atttatggat
    34861 ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc gggcgtaccg
    34921 gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac gtggcgatta
    34981 aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa cttactgaat
    35041 aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat cgtcagctgg
    35101 cagagtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa cggaagagta
    35161 cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt gacctttcag
    35221 ctcaatggct ttgttggtta ccacttgcag ctgcggatcg cggctacgtt gctgatcttc
    35281 cagacgcttc gccaacaccg ccagatcttc tttcggatca tcgcccatga tgacgatgac
    35341 tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg ttcccagctt
    35401 accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat caagcaggtt
    35461 gacgggattc ccggtagcgt tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc
    35521 tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat atttaaccag
    35581 attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca tccatcttat
    35641 cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc
    35701 cagtcttttc aacagaatat tgagtaatac gccatacatt ggcaggaaga aaacgatact
    35761 gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt cagccataaa
    35821 ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc tgacgttacc
    35881 gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca ggcggttaaa
    35941 aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg tggcgatacg
    36001 ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac ccatatagaa
    36061 tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa agatgatacg
    36121 acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga aaataaacgg
    36181 gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa gctgaaccag
    36241 atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca acgccttata
    36301 gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat tggggtgagg
    36361 gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta cccttttttc
    36421 gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc acgcaatcgt
    36481 taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt tatgtgatga
    36541 gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac ttggcctgcg
    36601 ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc ctggcgaaac
    36661 gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt tttgtacctt
    36721 tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt atttgattcg
    36781 taaaggcggt tgatggggaa agattggctt cgatgccgcc ttttcccctc accctaaccc
    36841 tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct
    36901 cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg tatctgccag
    36961 caccgccagc cacaggccgg tcatccctaa cagcgtggtg acgaggaaga tccctttcag
    37021 ccccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg ccagttcaat
    37081 catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg tttccagcgc
    37141 cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc
    37201 gttaatacca tcaccgacca tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt
    37261 gactttatct tccggcaata ggcccgcttt aaactccagc cccagctccc cggcgattgc
    37321 cgccgctgca cgtggattat cgccggtgag gatcactcct ttgaccccca gcgcgttcag
    37381 ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa tgatacccag
    37441 cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca gttcattaat
    37501 caaaccagca aatgcatcag cgggatgttt cccggcagcg caaatcaata cgcgctcacc
    37561 gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg cggtgggaat
    37621 ggcgagttca gcaacctgtg cttcgcgtac gatggcttgc gccagtggat gcgtcgcgcc
    37681 ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg ttgccggatg
    37741 aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa acgccacctg
    37801 agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc cacgacgcgc
    37861 tgccgccgcc agcccggagg taatcgccgc aggcgttgag ataactaacg cacacgggca
    37921 gccaatcagc agcagcgtca gccctttata aatccactcc tgccagctgg cggcaaacag
    37981 cagcggtggc accagcgtca ccagcagagc gacggccata atcgcgggcg tatagatacg
    38041 gctgaaacgg tcgataaacc gctcaatggg ggcgcgacgc tcttcggctt cttcgatcag
    38101 tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca gcgttaccag
    38161 acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat
    38221 ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca gtttaccgtc
    38281 ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca ggctgttaat
    38341 cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat
    38401 taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc gttcaccaat
    38461 caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat
    38521 agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga tcagccgtaa
    38581 tgcctgacga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg ccagttgccc
    38641 gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga ttagtgtaat
    38701 cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg
    38761 cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt cattgtcggc
    38821 atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac ctgcaagctg
    38881 gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac tgactttcca
    38941 gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc atgcgccgtc
    39001 gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag caaattgagg
    39061 ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag ttttttctca
    39121 ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag agatacagtg
    39181 agcggatgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg ttatccgcac
    39241 ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac agcaacgaag
    39301 cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt tcacctgcca
    39361 gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga tactcttcca
    39421 gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc agtggcagag
    39481 gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc aggtgcgaga
    39541 ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt agcgcatcgc
    39601 ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg aacatcggcg
    39661 cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc cagcgttgcc
    39721 aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag acagcgataa
    39781 acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga atgcctacgg
    39841 tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact ggcagggtat
    39901 gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct tttctttatt
    39961 gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa
    40021 aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag caacaagcgg
    40081 gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc aggcaccagt
    40141 atgcgctacg aggcaagctt caaaccgcaa agcggaggaa tggagcagac gtttcgcctc
    40201 gacgcccagc agtaccacgc cctgacagtg ggcgataaag gtacgctgag ctataaagga
    40261 acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt aaatttcttc
    40321 tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gtaccttttc ggcggtggtc
    40381 atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat accatgcatc
    40441 agcacggtaa aaatcagcgc cacgttaacg aaaatattta gcgggcgcgg gaagggatgc
    40501 accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc aatattaatc
    40561 agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct gacccgccac
    40621 tttttcccga tgtaacgacc agtttgccgg aacggtgggc agaccgtttt cgacttcgct
    40681 ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta aatttatcgt
    40741 ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat gcggtgtgcc
    40801 tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc ctgcttttag
    40861 tgtcgccaga ttcttaatta actgctgaga aactgcacga tccatctcaa ttaacgtggc
    40921 ccccgcagcg tagcgcgata acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc
    40981 cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaacgttt cgcgtacgcg
    41041 gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc ctcgccactg
    41101 cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca tgataaattg
    41161 ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt aagtgataga
    41221 ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc atgaagcaac
    41281 agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt tcctggctgg
    41341 gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat gaacaaccgg
    41401 ttgtagaaga aatcgttcag gcgcaagagc ctgtgaaggc ttcggaacaa gccgttgaag
    41461 agcagccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac gttgtggaag
    41521 tcactgaaca ggttgttgaa agtgaaaaag cgcagcctga agcggaagtt gttgcacagc
    41581 cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa gagctgccgt
    41641 tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct gaagcggaaa
    41701 ccgtagagat tgtcaaagcg gcggaagaag aagcggctaa agaagaaatc accgacgaag
    41761 agctggaagc acaggcgctg gcagccgaag cagcagaaga ggcggtgatg gtggttcctc
    41821 cggcagaaga agagcagccg gtggaagaaa tcgctcagga gcaggaaaaa ccaaccaaag
    41881 aaggtttctt cgcgcgcctg aaacgcagcc tgttaaaaac caaagaaaat ctcggttccg
    41941 gatttatcag cctgttccgc ggtaaaaaaa tcgacgatga tctgtttgag gagctggaag
    42001 agcagctttt gatcgccgat gtgggcgtgg aaaccacacg taaaattatc accaatctga
    42061 cggaaggcgc atcccgcaag cagcttcgtg atgccgaggc gctctatggc ctgctgaaag
    42121 aagagatggg tgagattctg gcgaaagtcg atgagccgct gaatgttgaa ggtaaaacgc
    42181 cgttcgtgat cctgatggtg ggcgtcaacg gtgtgggtaa aaccacgacg attggtaagc
    42241 tggcacgtca gtttgagcag cagggtaaat cggtgatgct ggcggcgggc gatactttcc
    42301 gtgcagcagc ggttgaacag cttcaggtct ggggtcagcg caacaatatt ccggtgattg
    42361 cccagcatac cggggcggat tccgcctctg ttatcttcga cgccattcag gcagctaaag
    42421 cgcgtaatat cgacgtcctg attgccgata ccgccggacg cctgcagaac aaatcgcacc
    42481 tgatggaaga gttgaagaaa atcgtccgcg tgatgaagaa actcgacgtt gaagcgccgc
    42541 atgaagttat gctgactatt gatgccagca ccgggcagaa cgcggtaagc caggccaaac
    42601 tgttccacga agctgttggc ttaaccggca tcacgctaac gaaactggac ggcacggcga
    42661 aaggcggggt aattttctcg gtggctgacc agtttggtat ccctatccgc tacattggtg
    42721 tcggcgaacg tattgaggat ttgcgtccgt ttaaggcgga cgactttata gaggcacttt
    42781 ttgcccgaga ggattaacaa tgattcgctt tgaacatgtc agcaaggctt atctcggtgg
    42841 gagacaggcg ctgcagggcg ttacgttcca tatgcagccg ggtgagatgg cgtttctgac
    42901 cggtcattcc ggcgcaggga aaagtaccct cctgaagctg atctgtggga ttgagcggcc
    42961 cagcgccggg aaaatctggt ttagcggcca tgacatcacg cgtctgaaaa accgtgaagt
    43021 tccgtttctg cgccgccaga ttggcatgat tttccaggat caccatctac tgatggaccg
    43081 tactgtctac gataacgtgg cgatcccgct gattatcgct ggtgccagcg gtgacgatat
    43141 tcgtcgccgg gtgtcggcgg cactggataa agtcgggcta ctggacaaag cgaagaactt
    43201 ccctattcag ctttccggcg gtgaacaaca gcgtgttggc attgcccgcg cggtggtgaa
    43261 taagcccgcg gtactgctgg cggacgaacc gaccggtaac ctggacgacg cgttgtcgga
    43321 agggattcta cgtctgtttg aagagtttaa ccgcgttggg gtaaccgtat tgatggcaac
    43381 gcacgacatc aacctgattt cacggcgttc ccatcgcatg ctcaccctga gcgatggtca
    43441 cttgcatgga ggcgtgggcc atgaataagc gcgatgcaat caatcatatt cggcagtttg
    43501 gtgggcgtct ggatcgcttc cgtaaatcgg tcggcggctc aggcgacggc ggtcgtaacg
    43561 caccgaaacg cgcgaaatcc tcgccaaaac cggtaaatcg caaaaccaac gttttcaacg
    43621 aacaggtgcg ctatgccttc cacggcgcat tgcaggatct gaaaagcaaa ccgttcgcca
    43681 cgtttttaac ggtgatggtt atcgccatct ccctgacgct gcccagcgtc tgttatatgg
    43741 tgtacaaaaa cgttaatcag gcggcgacgc agtattatcc gtcaccgcaa atcactgttt
    43801 acttgcaaaa aacgctggac gatgacgccg ctgcgggcgt ggtggcacag ttgcaggccg
    43861 agcaaggcgt ggagaaagtg aactatcttt ctcgtgaaga cgcactgggc gagttccgta
    43921 actggtctgg ttttggtggt gcgctggata tgctggaaga aaacccgctt ccggcagtgg
    43981 cggtggtgat cccgaaactc gattttcagg ggacggaatc actcaatacg ctgcgtgatc
    44041 gtatcacgca gattaacggc attgacgaag tgcggatgga tgacagctgg tttgcccgtc
    44101 tggcggcgtt gaccgggctg gtcgggcgcg tttcggcgat gatcggcgtg ttgatggtgg
    44161 cggcagtgtt cctcgtcatc ggcaacagcg tacgtctgag catctttgct cgccgtgact
    44221 ccatcaacgt gcagaaactg attggtgcga cggatggttt catccttcgc ccgttcctgt
    44281 atggtggcgc actgctggga ttttctggcg cattgttgtc attaattttg tcagaaattc
    44341 tggtgctgcg attgtcatcg gcggttgcgg aagtggcaca ggttttcgga acgaagtttg
    44401 atatcaatga tttatcgttc gatgaatgcc tgctattgct gctggtatgc tcgatgattg
    44461 gctgggtggc agcgtggctt gccacggtac aacatttacg ccactttacg cctgaataat
    44521 aaaagcgtgt tatactcttt ccctgcaatg ggttccgtag cagggaaaga gatcccgttg
    44581 tctcttcccg gtatttcatc tctatgtcac attttgtgcg taatttattc acaagcttgc
    44641 attgaacttg tggataaaat cactgtctga taaaacagtg gatgataacc tcgttgctct
    44701 taagctctgg cacagttgtt gctaccactg aagcgccaga agatatcgat tgagaggatt
    44761 tgaatgactg acaaaatgca aagtttagct ttagccccag ttggcaacct ggattcctac
    44821 atccgggcag ctaacgcgtg gccgatgttg tcggctgacg aggagcgggc gctggctgaa
    44881 aagctgcatt accatggcga tctggaagca gctaaaacgc tgatcctgtc tcacctgcgg
    44941 tttgttgttc atattgctcg taattatgcg ggctatggcc tgccacaggc ggatttgatt
    45001 caggaaggta acatcggcct gatgaaagca gtgcgccgtt tcaacccgga agtgggtgtg
    45061 cgcctggtct ccttcgccgt tcactggatc aaagcagaga tccacgaata cgttctgcgt
    45121 aactggcgta tcgtcaaagt tgcgaccacc aaagcgcagc gcaaactgtt cttcaacctg
    45181 cgtaaaacca agcagcgtct gggctggttt aaccaggatg aagtcgaaat ggtggcccgt
    45241 gaactgggcg taaccagcaa agacgttcgt gagatggaat cacgtatggc ggcacaggac
    45301 atgacctttg acctgtcttc cgacgacgat tccgacagcc agccgatggc accggtgctc
    45361 tatctgcagg ataaatcatc taactttgcc gacggcatcg aagatgataa ctgggaagag
    45421 caggcggcaa accgtctgac cgacgcgatg caaggtctgg acgaacgcag ccaggacatc
    45481 atccgcgcgc gctggctgga cgaagacaac aagtccacgt tgcaggaact ggctgaccgt
    45541 tacggtgttt ccgctgaacg tgtacgccag ctggaaaaga acgcgatgaa aaaactgcgc
    45601 gctgctatag aagcgtaatt tccgctatta agcagagaac cctggatgcg agtccggggt
    45661 ttttgttttt tgggcctcta caataatcaa tttcccctcc ggcaaaacgc caatccccac
    45721 gcagattgtt aataaactgt caaaatagct attccaatat cataaaaatc gggtatgttt
    45781 tagcagagta tgctgctaaa gcacgggtag ccatgcataa aacgaaataa agtgctgaac
    45841 aacaacatca caacacacgt aataaccaga agaatgggga ttctcaggat gaacataaag
    45901 ggtaaagcgt tactggcagg atgtatcgcg ctggcattca gcaatatggc tctggcagaa
    45961 gatattaaag tcgcggtcgt gggcgcaatg tccggtccgg ttgcgcagta cggtgaccaa
    46021 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctaaaggcgg cattaaaggc
    46081 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc ggttgcggtg
    46141 gcgaacaaag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
    46201 acgcagcctg cgtctgacat ctacgaagac gaaggcattt taatgatcac cccagcggca
    46261 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
    46321 tccgaccagg ggccgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
    46381 gctatcgttc acgacaaaca gcaatacggc gaaggtctgg cacgagcggt gcaggacggc
    46441 ctgaagaaag gcaatgctaa cgtggtgttc tttgatggta tcaccgccgg ggaaaaagat
    46501 ttctctacgc tggtggcgcg tctgaagaaa gagaatatcg acttcgttta ctacggcggt
    46561 taccacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
    46621 tttatggggc cggaaggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
    46681 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcaaa caaacccatt
    46741 gttgacgcga tcaaagcgaa aaaacaggac ccaagcggcg cattcgtttg gaccacctac
    46801 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
    46861 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacgtg ggatgagaaa
    46921 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgaca
    46981 gatgcgaagt aatcattaat cggcaacttt gggttgccgc caaattgcta atatcgagta
    47041 cgttgcttca tgccggatgc ggcgtaaacg ccttatccgg cctataagat ccaaagaaat
    47101 cagtaaattg caacacacat tgtaggcctg ataagcgtag cgcatcaggc aatttctcgt
    47161 tggtcatcac ttttgaaatc aacttgacga ttaacgcttc tcccagccgc cctgttgcgc
    47221 cgtaaatccc agtgcctgca taaacgctgt catcacaccg cgatcttcca cgcctgcatc
    47281 cgccatccac cagcatgaaa cgccaggatt gttacgcaaa acctcttcca gcagatattg
    47341 ccccacaccg cgacgtcggg tgacttcccg cacacgcagg gaatccagtg ctccctcggt
    47401 gccgcttaag gttacccgta cggcagcgag caggcgctcg ttaaaacgcg cggcgtagat
    47461 acggtggtta tcgtcaacct gtaaagagga aggggaatac tccggccaga tcttttgcag
    47521 gtcaatccgg tcctggtcgc taaagttttc taatcgaatg atggtcagct tcatgggtaa
    47581 cccgtgtaaa tcacaaaagt ggaaccagtg tagcgaaata atttaatcgg aggctttctc
    47641 ttttttattt cttttggcag gtgattaatt ttttaacagc aataattaca aagttaaaac
    47701 attagagaat gaaaaatacc cagcataatc ccctgaatgg tagtgaatta ttccgccctt
    47761 tgtgccgtta ttttatgctg acaaaggcac ttttttctgt ttatctatca ataaattcag
    47821 aatattatct gttcttaatc gactgaaaaa tagggatttt aatcactgtt atcacaaaat
    47881 actgcgctaa acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct
    47941 gatttgttaa cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg
    48001 aaacggaatg cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct
    48061 atggctgacg atattaaagt cgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg
    48121 ggcgatatgg aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga
    48181 attaagggcg acaagctggt tggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc
    48241 gttgcggtcg ccaacaaaat cgttaatgac ggcattaaat acgttattgg tcatctgtgt
    48301 tcttcttcta cccaacctgc atcagatatc tacgaagacg aaggtattct gatgatctcg
    48361 ccgggagcga ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc
    48421 gggctggact cttcccaggg gccaacggcg gcaaaataca ttcttgagac ggtgaagccc
    48481 cagcgcatcg ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg
    48541 caggacgggc tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg
    48601 gagaaagatt tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac
    48661 tacggcggtt actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg
    48721 aaaactcagt ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc
    48781 gatgctgccg aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac
    48841 cagggcatcg ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg
    48901 atcacctacg cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag
    48961 ccgctggcgc tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg
    49021 aactgggatg aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc
    49081 gatggttcat ccacggcagc caagtgatca tcccaccgcc cgtgaaatgc gggcgggttt
    49141 agaaaggtta ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg
    49201 tcacgctggg cagtacctac gcgctgatag ccatcggcta caccatggtt tacggcatta
    49261 tcggcatgat caacttcgcc cacggcgagg tttacatgat tggtagctat gtctccttta
    49321 tgatcatcgc cgcactgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg
    49381 gattcgtcgg cgcaatcgtc attgccagcg cctacggctg gagtatcgaa cgggtagcct
    49441 atcgcccggt gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca
    49501 tcttcctgca aaactacgtt agcctgaccg aaggttcgcg tgacgtggcg ctgcccagtc
    49561 tgtttaacgg tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca
    49621 tgcaggcagt gatctggatt gttactttcc tcgccatgct ggcgctgacg attttcattc
    49681 gttattcccg tatggggcgc gcctgccgtg cctgcgcgga agatctgaaa atggcgagcc
    49741 tgcttggcat caacaccgac cgggtgattg ctttgacctt tgttattggc gcggcgatgg
    49801 cggcagtggc tggcgtactg ctcggtcagt tctacggcgt cattaacccc tatatcggct
    49861 ttatggccgg gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccag
    49921 gggcgatgat tggcgggctg atcctgggga ttgcggaggc gctctcttct gcctatctga
    49981 gtacggaata taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc
    50041 cgaccggtat tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat
    50101 ggcactgctc tctgccgcga tgttctttgt cctcgctggc gtctttatgg gcgtgcaact
    50161 ggagctggat ggcaccaaac tggtggtcga cagcgcttcg gatgtccgtt ggcagtgggt
    50221 gtttatcggc acggcggtgg tctttttctt ccagcttttg cgacctgctt tccagaaagg
    50281 gctaaaaagc gtttccgggc cgaagtttat tctgcccgcg attgatggct ccacggtgaa
    50341 gcagaaactg ttcctcgtgg cgctgctggt gcttgcggtg tcgtggccgt ttatggtgtc
    50401 acgtgggacg gtggatatcg ccactctgac catgatctac attatcctcg gtctcgggct
    50461 gaatgtggtt gttggtcttt ctggtctgct ggtgctgggg tacggcggtt tttacgccat
    50521 cggcgcttac acttttgcgc tgctcaatca ctattacggt ctggggttct ggacctgttt
    50581 gccgattgcc ggactgatgg cagcggcggc gggcttcctg cttggttttc cggtgttgcg
    50641 tctgcgcggt gattatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt
    50701 gctgctcaat aacaccgaaa ttaccggcgg cccgaacggt atcagtcaga tcccgaaacc
    50761 gactttcttc ggccttgagt tcagccgtac cgcccgtgaa ggcggttggg acacgttcag
    50821 taatttcttt ggcctgaaat acgatccctc cgatcgcgtc atcttcctgt atctggtggc
    50881 gttgctgctg gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg
    50941 gcgtgcgtgg gaagcgttgc gtgaagatga gatcgcctgc cgttcgctgg gcttaagccc
    51001 gcgtcgtatc aagctgactg cctttaccat cagtgccgcg tttgccggtt ttgccggaac
    51061 gctgtttgct gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc
    51121 gtttgtactg gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc
    51181 ggcaattttg ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt
    51241 aatgctcggt ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat
    51301 gacgcgcccg caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag
    51361 ccattattat ctgtgaacgg cctgatgatg cgcttcggcg gcctgctggc ggtgaacaac
    51421 gtcaatcttg aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga
    51481 aaaaccacgg tttttaactg tctgaccgga ttctacaaac ccaccggcgg caccatttta
    51541 ctgcgcaatc agcacctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg
    51601 cgcaccttcc agcatgtgcg tctgttccgt gaaatgacgg taattgaaaa cctgctggtg
    51661 gcgcagcatc aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc
    51721 cgtcgcgccc agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg
    51781 ctggaacacg ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag
    51841 attgcccgct gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt
    51901 cttaacccaa aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac
    51961 aacaccacta tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga
    52021 atttatgtgg tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat
    52081 aacccggacg tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc
    52141 ctttgacaaa gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcctgca
    52201 tatcaatcag ggcgagattg tcaccctcat tggcgcgaac ggggcgggga aaaccacctt
    52261 gctcggcacg ttatgcggcg atccgcgcgc taccagcggg cgaattgtgt ttgatgataa
    52321 agacattacc gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga
    52381 agggcgtcgc gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttttt
    52441 tgccgaacgc gaccagttcc aggagcgcat aaagtgggtg tatgaactgt tcccacgtct
    52501 gcatgagcgc cgtgttcagc gggcaggcac catgtccggc ggtgagcaac aaatgctggc
    52561 gattggtcgc gcgctgatga gcaacccgcg tttgctgcta cttgatgagc catcgcttgg
    52621 tcttgcgccg attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg
    52681 gatgactatc ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg
    52741 ctacgtgctg gaaaacggcc atgtggtgct ctccgatact ggtgatgcac tactggcgaa
    52801 tgaagcggtg agaagtgcgt atttaggcgg gtaataacac gttgattgat aaggagtcaa
    52861 aagactcctt tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc
    52921 ctactgggtg agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt
    52981 acgccgcatc cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt
    53041 ttccgcttat ccgtgcccca tctcccattt tccctcaccc acgccgtcac cgccttgtca
    53101 tctttctgac accttacttg caggtttatg aagttggcac cgccaccatg atggcatcga
    53161 aagccattaa accggtatat gacgtgttta aagaggcagg gattcaattc gatgagtcgc
    53221 agtttgtgcc gacggtttcc ggttactact ccgacagcaa aacgggccac ttactctccc
    53281 agccattcaa cagctcgact cccgttctct attacaacaa agacgccttc aagaaagcgg
    53341 ggttagaccc ggaacagccg ccgaaaacct ggcaggatct ggcggactct tccgcaaaac
    53401 tgaaagcctc cggtatgaag tgcggctacg ccagcggctg gcagggctgg atccaactgg
    53461 aaaactttag cgcctggaac ggtctgccgt ttgccagcaa aaacaacggc tttgacggca
    53521 cggacgcggt gctggagttc aacaagccgg agcaggtgaa acacattgcc atgctcgaag
    53581 agatgaacaa gaagggcgac ttcagctacg tcggtcgtaa ggatgaatcc accgagaagt
    53641 tctataacgg tgattgcgcg atgaccaccg cctcttccgg ttctctcgct aatattcgtg
    53701 agtacgccaa atttaactat ggcgtaggca tgatgcctta cgacgccgat gcgaaagatg
    53761 caccgcaaaa cgccattatc ggcggggcca gcctgtgggt gatgcagggc aaaaataaag
    53821 aaacgtatac cggcgtggcg aagttcctcg acttcctcgc gaagccagaa aacgctgccg
    53881 agtggcatca gaaaaccggt tatctgccaa tcaccaaagc agcgtatgac ctgacccgtg
    53941 agcagggctt ttatgagaaa aacccagggg cggataccgc gacgcgtcag atgctgaata
    54001 agccgccgtt gccgttcacc aaagggctgc gtctgggcaa catgccgcag atccgcgtga
    54061 ttgtggatga agagctggag agcgtgtgga ccggtaagaa gacaccacag caggcactgg
    54121 ataccgccgt tgagcgtgga aatcagttgc tgcgccgctt tgagaaatcg acgaagtctt
    54181 aatcagtgta atgtcggatg cgtttcgctt atctgacctg gcatcgcgtg taggccggat
    54241 aagcgaagcg catccggcac agttcaggaa ttaaccgtaa tgtcatcatc ccgtccggtg
    54301 ttccgctcgc gctggctgcc ttatctgctg gtcgcgccgc agctcatcat caccgttatc
    54361 ttttttatct ggcctgcggg cgaagcgttg tggtactcgc ggcaaagcgt cgatccgttt
    54421 ggtttctcca gccagtttgt cggtctggat aacttcgtca cgctgttcca tgacagctac
    54481 tatctagact ccttctggac gacgataaaa ttcagcacct ttgtcaccgt cagcggtttg
    54541 ctggtatcgc tgttctttgc ggcgctggtg gagtacatcg tgcgcggcag ccgtttctat
    54601 caaaccttga tgctgctgcc gtatgccgtg gctcccgccg ttgccgccgt attgtggatc
    54661 ttcctgttta accccggtcg cgggctgatc acccattttc tcgctgagtt cggctacgac
    54721 tggaaccacg cgcaaaacag cggtcaggca atgtttctgg tggtgtttgc ctcggtgtgg
    54781 aagcaaatca gctacaactt tctgttcttc tatgccgcgc tgcactccat tccccgttcg
    54841 ttgattgaag ccgcagctat tgacggcgca gggccgattc gccgcttctt taagattgct
    54901 ctgccgctta tcgctccggt gagtttcttc ctgctggtgg tgaacctggt gtacgccttc
    54961 ttcgacacct tcccggtgat cgacgccgcc acgtccggcg ggccggttca ggccaccact
    55021 acgctgattt ataagatcta ccgcgaaggc tttaccggac tggatctggc ttcgtctgcc
    55081 gcgcagtcgg tggtgttgat gttcctcgtc atcgtgctga cggtggtgca gttccgctat
    55141 gttgaaagca aggtgcgtta ccaatgattg agaaccgtcc gtggctgacg atattcagcc
    55201 ataccatgct gatcctcggg atcgcggtga tcctcttccc gctgtacgtg gcgtttgtcg
    55261 cggcgacgct ggataaacag gccgtctatg ccgcgccgat gacgctcatc cccggcacac
    55321 atctgctgga aaatatccac aacatctggg ttaacggggt aggcacgaat agcgcgccgt
    55381 tctggcggat gttgcttaac agctttgtga tggcgttcag cattacgctc ggcaaaatta
    55441 ccgtctcgat gctctcggca tttgccattg tctggtttcg ttttccgcta cgtaacctct
    55501 tcttctggat gatttttatc accctgatgc tgccggttga agtacgtatc ttcccgacgg
    55561 tggaagtcat cgccaacctg aaaatgctcg acagctacgc cggtttaacg ctgccgctga
    55621 tggcctcggc gaccgccacg tttctgttcc gccagttctt tatgacccta ccggatgaat
    55681 tggtggaagc cgcgcggatc gacggcgcgt caccaatgcg tttcttttgc gacatcgttt
    55741 ttccgttgtc caaaactaat ctggcggcgc tgtttgtgat cacctttatc tacggctgga
    55801 atcagtattt gtggccgttg ttgattatta ccgacgttga tctcggcacc accgtggcag
    55861 ggatcaaagg gatgatcgct acaggcgaag gcaccacgga atggaactcg gtaatggcgg
    55921 cgatgttgtt aacgcttatc cctccggtgg tgattgtttt agtgatgcag cgtgccttcg
    55981 tgcgcggcct ggtcgatagt gagaaataag atggcaggac tgaaattaca ggcagtaacc
    56041 aaaagctggg atggcaaaac ccaggtcatt aaaccgctga cccttgatgt ggcggatggc
    56101 gaatttatcg tgatggtcgg gccgtctggc tgcgggaaat cgacgctgct gcgcatggtt
    56161 gccgggctgg agcgggtgac agaaggcgat atctggatca acgaccagcg cgtgaccgaa
    56221 atggagccga aagatcgcgg gattgcgatg gtgttccaga attacgcgct ttatccgcat
    56281 atgagcgtag aggagaacat ggcgtggggg ctgaaaattc gcggcatggg caagcagcaa
    56341 attgccgagc gcgttaaaga agcggcgcgc attctggaac tggacggtct gcttaagcgt
    56401 cgtccgcgtg agctatccgg cggtcagcgc cagcgtgtgg cgatgggccg cgcgattgtg
    56461 cgcgatccgg cggtgttctt gtttgatgag ccgctctcta acctcgatgc caagctgcgc
    56521 gtgcagatgc gtcttgaact gcaacagttg caccgtcgcc tgaaaacgac ttcactctac
    56581 gttactcacg atcaggttga agcgatgacg ctcgcccagc gagtaatggt gatgaacggt
    56641 ggcgttgccg aacagattgg cacaccagtt gaagtctacg aaaagcccgc cagcctgttt
    56701 gtggcgagtt ttatcggcag cccggcgatg aacctgctga caggccgcgt gaataatgaa
    56761 ggcacgcatt tcgaactgga cggcggtatc gtgctaccgc taaacggtgg ctaccgtcag
    56821 tatgccgggc gtaaaatgac tctcggcatt cgcccggaac atatagcgct aagctcgcag
    56881 gcagaaggtg gtgtgccgct ggtgatggac acgctggaga tcctcggcgc agataacctg
    56941 gcgcacggac gctggggcga gcagaagctg gtggtgcgac tggcgcatca ggagcgcccg
    57001 acggcaggca gcacgctgtg gctgcatttg gcggaaaatc agctgcatct ttttgatggt
    57061 gaaacaggac aacgagtatg agtaactggc cttatccccg catcgtcgct catcgtggcg
    57121 gcggtaagct ggccccggaa aacaccctgg cggcaatcga cgtcggggca aaatacggtc
    57181 ataagatgat cgaatttgac gcgaagttat cgaaagatgg cgagatcttc ctgctccatg
    57241 acgacaatct cgaacgcacc agcaacggct ggggcgtcgc gggtgaactg aactggcagg
    57301 atttactgcg cgtggatgcg ggcagttggt acagcaaagc gtttaaaggt gagccgctgc
    57361 cgttgctttc gcaggtggcg gaacgctgtc gcgaacacgg gatgatggcg aatatcgaaa
    57421 tcaaacccac cactggcact ggaccattaa cgggcaaaat ggtggcgctg gcggcacgcc
    57481 aactgtgggc cggtatgacg ccgccgctgc tgtcatcgtt tgagattgat gctttagaag
    57541 ctgcacaaca ggcggcaccg gaactgccgc gtggtttatt gcttgatgag tggcgcgacg
    57601 actggcgcga actgaccgcg cggctgggct gcgtctctat tcatctcaat cataagttac
    57661 tcgataaagc gcgagtgatg cagttgaaag acgccggatt acggattctg gtttataccg
    57721 tcaataaacc ccagcgcgcg gcagagttgc tgcgctgggg cgtggattgc atctgtaccg
    57781 atgcgattga cgtgattggt ccgaacttta cggcccaata gttttcaacg gaatgtcagg
    57841 ctgcggcgtg ctgggctgat tcagcatgtc gccgtttctc ctttccggca gcatatgctg
    57901 ctgattcaac gaactatccg gattacggtt gctgtttaac atcccgccgt tggtgttggg
    57961 cagcatttgc tgccgggcgg gatttcgctc ccccggctgt gactgcaaca cccgctgaga
    58021 attattgttt atctggtttt ctaaatgctg ctgttgcaac tgagtttgcg ttttcagttg
    58081 ctgattcagc atcccttttt gctggatttg ctgagtctgc atctgggttt gcatccgctg
    58141 ctggctgggt atctgatacc ccggctggtt agggttgttc agagtattaa tgggctgtgc
    58201 aaagccgaca aacggcagga gtgccgtaag aatcagaagt cgtttcatcg cgtatcctcc
    58261 tctgaagata tcctttaagt ttactcgctt cccgacaaaa cgatgattaa ttcagagtta
    58321 tataccaggc ttagctgggg ttgcccctta atctctggag aataacgatg ataaaaccga
    58381 cgtttttacg ccgggtggcc attgctgctc tgctctcagg aagttgtttt agcgccgccg
    58441 ccgcgcctcc tgcgccgccc gtctcgtatg gtgtggagga agatgtcttc cacccggtac
    58501 gcgcgaaaca gggaatggta gcgtctgtgg acgccactgc cactcaggtg ggggtggata
    58561 tcctcaagga gggcgggaat gccgttgatg ccgccgtggc ggtgggctac gcgctggcgg
    58621 taacgcatcc gcaggcaggg aatctgggcg gtggtggttt tatgttaatc cgctcgaaaa
    58681 atggcaatac cacggctatc gatttccgcg aaatggcacc cgccaaagcg acccgcgata
    58741 tgttcctcga tgatcagggc aacccggaca gcaaaaaatc actcacttcg catctggctt
    58801 ccggcacacc gggtacggta gcaggtttct cgctggcgct ggataaatac ggcaccatgc
    58861 cgctgaacaa agtcgtgcag cccgcgttta aactggcacg cgatggtttt atcgttaacg
    58921 acgcgctggc tgacgatctc aaaacctacg gtagcgaagt gttgccgaat cacgaaaaca
    58981 gtaaagctat cttctggaaa gagggcgagc cgctgaaaaa gggcgacacg ctggtgcagg
    59041 cgaacctggc aaagagcctg gagatgattg ctgaaaacgg cccggacgaa ttctataaag
    59101 gcacgattgc ggaacagatc gcccaggaga tgcagaaaaa cggtggcttg atcactaaag
    59161 aagatttagc ggcctataaa gcggtcgaac gcactccgat aagcggcgat tatcgcgggt
    59221 atcaggttta ctccatgcca ccgccatcct ccggcgggat ccatatcgta caaatcctca
    59281 atattctgga aaacttcgat atgaagaaat acggctttgg cagcgccgat gcgatgcaaa
    59341 tcatggcaga agcggagaaa tacgcctacg ccgaccgctc ggaatatctt ggcgacccgg
    59401 attttgtcaa agtcccgtgg caggcactga ccaataaagc ctatgccaaa tccattgccg
    59461 atcaaattga tatcaacaaa gcgaagccgt ccagtgagat tcgccctggc aagcttgcgc
    59521 cttatgagag taatcaaact acccattact ctgtagtgga taaagacggt aacgcggtgg
    59581 cggtgaccta tacgctgaac accaccttcg gtacgggcat tgtcgcgggc gagagcggta
    59641 ttctgcttaa taatcagatg gatgatttct ccgccaaacc gggcgtaccg aacatttacg
    59701 ggctggtggg cggtgatgcc aacgccgtcg ggccgaataa acgtccgctg tcatcgatgt
    59761 caccaaccat tgtggtgaaa gacggtaaaa cttggctggt taccggtagt cctggcggga
    59821 gtcggattat taccaccgtg c
//
LOCUS       DAJTVK010000010        58479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000010 DAJTVK010000000
VERSION     DAJTVK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.010"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            90..254
                     /locus_tag="OO372_000667"
                     /pseudo
     CDS             90..254
                     /locus_tag="OO372_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            complement(292..1353)
                     /locus_tag="OO372_000668"
     CDS             complement(292..1353)
                     /locus_tag="OO372_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6340254.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1566..3029
                     /gene="bepA"
                     /locus_tag="OO372_000669"
     CDS             1566..3029
                     /gene="bepA"
                     /locus_tag="OO372_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6340255.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINNN
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            3050..3409
                     /gene="arsC"
                     /locus_tag="OO372_000670"
     CDS             3050..3409
                     /gene="arsC"
                     /locus_tag="OO372_000670"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6340256.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3547..4293)
                     /gene="hda"
                     /locus_tag="OO372_000671"
     CDS             complement(3547..4293)
                     /gene="hda"
                     /locus_tag="OO372_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6340257.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4343..5632)
                     /gene="uraA"
                     /locus_tag="OO372_000672"
     CDS             complement(4343..5632)
                     /gene="uraA"
                     /locus_tag="OO372_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6340258.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5718..6344)
                     /gene="upp"
                     /locus_tag="OO372_000673"
     CDS             complement(5718..6344)
                     /gene="upp"
                     /locus_tag="OO372_000673"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6340259.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6669..7706
                     /gene="purM"
                     /locus_tag="OO372_000674"
     CDS             6669..7706
                     /gene="purM"
                     /locus_tag="OO372_000674"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6340260.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7706..8344
                     /gene="purN"
                     /locus_tag="OO372_000675"
     CDS             7706..8344
                     /gene="purN"
                     /locus_tag="OO372_000675"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6340261.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8515..10581
                     /gene="ppk1"
                     /locus_tag="OO372_000676"
     CDS             8515..10581
                     /gene="ppk1"
                     /locus_tag="OO372_000676"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6340262.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10586..12127
                     /gene="ppx"
                     /locus_tag="OO372_000677"
     CDS             10586..12127
                     /gene="ppx"
                     /locus_tag="OO372_000677"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6340263.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12166..14409)
                     /gene="pdeF"
                     /locus_tag="OO372_000678"
     CDS             complement(12166..14409)
                     /gene="pdeF"
                     /locus_tag="OO372_000678"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6340264.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14591..14743)
                     /locus_tag="OO372_000679"
     CDS             complement(14591..14743)
                     /locus_tag="OO372_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340265.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14761..14952
                     /locus_tag="OO372_000680"
     CDS             14761..14952
                     /locus_tag="OO372_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6340266.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            15263..15781
                     /locus_tag="OO372_000681"
     CDS             15263..15781
                     /locus_tag="OO372_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6340267.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            15797..16336
                     /locus_tag="OO372_000682"
     CDS             15797..16336
                     /locus_tag="OO372_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6340268.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16431..18008)
                     /gene="guaA"
                     /locus_tag="OO372_000683"
     CDS             complement(16431..18008)
                     /gene="guaA"
                     /locus_tag="OO372_000683"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6340269.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(18077..19543)
                     /gene="guaB"
                     /locus_tag="OO372_000684"
     CDS             complement(18077..19543)
                     /gene="guaB"
                     /locus_tag="OO372_000684"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6340270.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            19705..21075
                     /gene="xseA"
                     /locus_tag="OO372_000685"
     CDS             19705..21075
                     /gene="xseA"
                     /locus_tag="OO372_000685"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6340271.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(21072..21287)
                     /locus_tag="OO372_000686"
     CDS             complement(21072..21287)
                     /locus_tag="OO372_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6340272.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(21356..22828)
                     /gene="der"
                     /locus_tag="OO372_000687"
     CDS             complement(21356..22828)
                     /gene="der"
                     /locus_tag="OO372_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6340273.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(22946..24124)
                     /gene="bamB"
                     /locus_tag="OO372_000688"
     CDS             complement(22946..24124)
                     /gene="bamB"
                     /locus_tag="OO372_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6340274.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(24135..24755)
                     /gene="yfgM"
                     /locus_tag="OO372_000689"
     CDS             complement(24135..24755)
                     /gene="yfgM"
                     /locus_tag="OO372_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6340275.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(24773..26047)
                     /gene="hisS"
                     /locus_tag="OO372_000690"
     CDS             complement(24773..26047)
                     /gene="hisS"
                     /locus_tag="OO372_000690"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6340276.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(26158..27276)
                     /gene="ispG"
                     /locus_tag="OO372_000691"
                     /gene_synonym="gcpE"
     CDS             complement(26158..27276)
                     /gene="ispG"
                     /locus_tag="OO372_000691"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6340277.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(27303..28316)
                     /gene="rodZ"
                     /locus_tag="OO372_000692"
     CDS             complement(27303..28316)
                     /gene="rodZ"
                     /locus_tag="OO372_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6340278.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(28601..29755)
                     /gene="trmG/rlmN"
                     /locus_tag="OO372_000693"
     CDS             complement(28601..29755)
                     /gene="trmG/rlmN"
                     /locus_tag="OO372_000693"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6340279.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(29905..30336)
                     /gene="ndk"
                     /locus_tag="OO372_000694"
     CDS             complement(29905..30336)
                     /gene="ndk"
                     /locus_tag="OO372_000694"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6340280.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(30485..32797)
                     /gene="pbpC"
                     /locus_tag="OO372_000695"
     CDS             complement(30485..32797)
                     /gene="pbpC"
                     /locus_tag="OO372_000695"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6340281.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(32798..37759)
                     /gene="yfhM"
                     /locus_tag="OO372_000696"
     CDS             complement(32798..37759)
                     /gene="yfhM"
                     /locus_tag="OO372_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR6340282.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            37966..38811
                     /gene="sseA"
                     /locus_tag="OO372_000697"
     CDS             37966..38811
                     /gene="sseA"
                     /locus_tag="OO372_000697"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6340283.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(39304..40080)
                     /gene="sseB"
                     /locus_tag="OO372_000698"
     CDS             complement(39304..40080)
                     /gene="sseB"
                     /locus_tag="OO372_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6340284.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(40222..41505)
                     /gene="pepB"
                     /locus_tag="OO372_000699"
     CDS             complement(40222..41505)
                     /gene="pepB"
                     /locus_tag="OO372_000699"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6340285.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(41683..41883)
                     /gene="iscX"
                     /locus_tag="OO372_000700"
     CDS             complement(41683..41883)
                     /gene="iscX"
                     /locus_tag="OO372_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6340286.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(41895..42230)
                     /gene="fdx"
                     /locus_tag="OO372_000701"
     CDS             complement(41895..42230)
                     /gene="fdx"
                     /locus_tag="OO372_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6340287.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(42232..44082)
                     /gene="hscA"
                     /locus_tag="OO372_000702"
     CDS             complement(42232..44082)
                     /gene="hscA"
                     /locus_tag="OO372_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6340288.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(44099..44614)
                     /gene="hscB"
                     /locus_tag="OO372_000703"
     CDS             complement(44099..44614)
                     /gene="hscB"
                     /locus_tag="OO372_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6340289.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(44710..45033)
                     /gene="iscA"
                     /locus_tag="OO372_000704"
     CDS             complement(44710..45033)
                     /gene="iscA"
                     /locus_tag="OO372_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6340290.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(45050..45436)
                     /gene="iscU"
                     /locus_tag="OO372_000705"
     CDS             complement(45050..45436)
                     /gene="iscU"
                     /locus_tag="OO372_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6340291.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(45464..46678)
                     /gene="iscS"
                     /locus_tag="OO372_000706"
     CDS             complement(45464..46678)
                     /gene="iscS"
                     /locus_tag="OO372_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6340292.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(46919..47407)
                     /gene="iscR"
                     /locus_tag="OO372_000707"
     CDS             complement(46919..47407)
                     /gene="iscR"
                     /locus_tag="OO372_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6340293.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(47708..48445)
                     /gene="trmJ"
                     /locus_tag="OO372_000708"
     CDS             complement(47708..48445)
                     /gene="trmJ"
                     /locus_tag="OO372_000708"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6340294.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            48564..49367
                     /gene="suhB"
                     /locus_tag="OO372_000709"
     CDS             48564..49367
                     /gene="suhB"
                     /locus_tag="OO372_000709"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6340295.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            49512..50366
                     /locus_tag="OO372_000710"
     CDS             49512..50366
                     /locus_tag="OO372_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6340296.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            50557..51837
                     /gene="csiE"
                     /locus_tag="OO372_000711"
     CDS             50557..51837
                     /gene="csiE"
                     /locus_tag="OO372_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6340297.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(51829..52968)
                     /locus_tag="OO372_000712"
     CDS             complement(51829..52968)
                     /locus_tag="OO372_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6340298.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(53128..54018)
                     /gene="hcaR"
                     /locus_tag="OO372_000713"
                     /pseudo
     CDS             complement(53128..54018)
                     /gene="hcaR"
                     /locus_tag="OO372_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            54154..55515
                     /locus_tag="OO372_000714"
     CDS             54154..55515
                     /locus_tag="OO372_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6340299.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            55512..56030
                     /locus_tag="OO372_000715"
     CDS             55512..56030
                     /locus_tag="OO372_000715"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6340300.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            56030..56350
                     /locus_tag="OO372_000716"
     CDS             56030..56350
                     /locus_tag="OO372_000716"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6340301.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            56347..57159
                     /gene="hcaB"
                     /locus_tag="OO372_000717"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             56347..57159
                     /gene="hcaB"
                     /locus_tag="OO372_000717"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6340302.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            57169..58371
                     /locus_tag="OO372_000718"
     CDS             57169..58371
                     /locus_tag="OO372_000718"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR6340303.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        14290 a        15812 c        14589 g        13788 t
ORIGIN      
        1 ctgcgacgtt ctgttcgcca tgatgcgcga cgggactttt tataccccgc aggggtcata
       61 acatgcttga caacttaata ggggcacccc ccttctggca actggcgcac agtagcgcag
      121 acaattttcc ggcactgacg gtaagccatt ttattaccgc caatctgctc ccggtgatgc
      181 tgggtaatat tatcggcggt gcagtgctgg tgagtatgtg ttatcgggct atttatttac
      241 gtcaggaatc ctgaaaaatc agcccggcga aacagttcgt cgggctgaag attattcttg
      301 cgctatttgc ccatcgggcc aggcgtggat cacggctttg atcagcgtcg ccaatggaat
      361 agcgaagaat acgccccaga atccccacaa accaccgaag atcaccaccg ataaaataat
      421 caccagcgga tgcaggttaa ccgcttcgga gaacaacacc ggtactaaca gattaccgtc
      481 cagcgcctga ataatcagat acactgcgaa gcagctccag aattccgtgc ctgcaccaaa
      541 ctggaatagc gccacgccaa ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat
      601 aagtaccgag aagccgacca gcaccgccag cagcagcgaa tagttcagcc caaagagcaa
      661 gaaccccagc caagtggcga tccccaccac gatcatctcc agcactttgc cgcggatata
      721 gttggtgatt tgttgattca tctccttcca cacctgtcct gccagtccac ggttgcgcgg
      781 cagcacccga cgaacggcgt tcagcatctg ctctttgtct ttcagcagga agaagaccat
      841 caatggtacc agcaccagat agacggcaat ggtcagcaaa ccgaccagcg aggcgaggga
      901 aattttcacc accgaatcgc ccatggtcag catccgactg cgcatatttt cggccattgc
      961 atcaataatg cccgcgtcca ttaatgccgg atagcggcgc ggcaacgtgg cggcaaagtc
     1021 agaaagctta ttgagcatcc ccggcatatc gcggatcaag tagatgccct gttgccaggc
     1081 gataggcagt accacgaacg ccatcagtag caatataccg acgaaaacca ccaatacaat
     1141 cgacgtcgcc cagcggcggg agcagccaat agattgcagg cgcacggttg gccattccag
     1201 caaataggcc agcacaatag ccaccagcaa cggagcaagc aggccactaa agaaaaagat
     1261 aatgccaaat ccggcaacta aaataaccag caaggcaatc gcttccgggt cgctaaaacg
     1321 gcggcgatac cattgcatca acatttcgag cataaagacc ttccctgaac ctcaagagcg
     1381 ggattgcgat ccgcaattgt atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga
     1441 ttcaggaaac tgaaaagtca tttgagtggg ctaatcttcg ccgttacact caaaggcggc
     1501 gcggtgggaa caatatttca cagtatcggt caaatgacta tcttcagaaa tacaggatag
     1561 aggttatgtt caggcagttg aaaaaaaacc tggttgcaac cctcattgct gctatgacca
     1621 ttggtcaggt agccccggcg tttgccgaca gcgcagacac cttgccggat atgggaacct
     1681 ccgcaggaag cacgctttcc attggtcagg aaatgcagat gggcgactat tatgtccgcc
     1741 agctacgcgg cagcgcgccg ttaattaatg acccgctgtt aacgcaatat attaattcgc
     1801 tggggatgcg tctggtttcg catgccaatt cggttaagac accgtttcat ttttttctga
     1861 tcaacaacaa cgaaattaac gcctttgctt tctttggcgg caacgtggtg ctgcactctg
     1921 ccctgttccg ttattccgat aacgaaagtc aactggcttc agttatggcg cacgaaatct
     1981 cccacgtcac ccaacgtcac ctggcgcgag cgatggaaga tcagcagcgc agcgcgccgc
     2041 tgacctgggt cggcgcgtta ggttctattt tactggcgat ggccagtccg caggcgggga
     2101 tggcggcgct gaccggtaca ctggcgggaa cgcgtcaggg gatgatcagt ttcacccagc
     2161 aaaatgaaca ggaagcggac cgcattggta ttcaggtgct gcaacgctcg ggattcgatc
     2221 cgcaggcgat gccaaccttc ctcgaaaaat tactcgatca ggcgcgttac tcctcgcgcc
     2281 cgccggaaat tttattgact cacccgttgc cggaaagtcg tctggcagat gcccgcaacc
     2341 gtgctaatca gatgcgcccg atggtggtgc agtcgtcgga agatttctat ctggcgaaag
     2401 cgcgcacact ggggatgtat aattccggac gtaaccagct caccagtgat ttgctggatg
     2461 aatgggcgaa aggaaacgtt cgtcagcaac gagcggcgca atatggtcgt gctttacagg
     2521 cgatggaagc caataaatac gacgaggcgc gtaaaacgct gcaaccgtta ctggcggcag
     2581 aacctggcaa cgcatggtat ctcgatctgg ctaccgatat cgatcttggg caaaacaaag
     2641 ccaatgaggc aatcaatcgc ctgaaaaatg cccgtgattt gcgcaccaat ccggtgttgc
     2701 agctcaacct ggcaaacgct tatctgcaag gcggtcaacc acaagaagcg gccaatattc
     2761 tgaatcgcta cacctttaat aataaagatg acagcaacgg ctgggattta ctggcacagg
     2821 cggaagccgc gctaaataac cgcgatcagg agctggctgc gcgagcagaa ggttatgcgc
     2881 tcgccggacg actcgatcag gccatttcgc tgttgagtag cgccagttcg caggtgaaat
     2941 taggcagcca gcaacaagcg cgttacgatg cgcgcatcga ccagttgcgc cagctgcagg
     3001 aacgctttaa gccttatacc aagatgtaat tcgcattaaa ggaagaacaa tgaccaaaca
     3061 ggtaaaaatc taccataacc cacgctgttc aaagagccgg gaaacgctga acctgctgaa
     3121 aaaaaacggc gtggagccag aagtggtact ctaccttgag acacccgccg atgcggcaac
     3181 gctgcgcgat ttgctgaaaa tgctggggat gaatagcgcc cgtgaattga tgcgccagaa
     3241 agaggatctt tataaagaac tgaacctggc tgacagttcg cttagcgaag aggcgctgat
     3301 tcaggcaatg gtggataatc cgaagctgat ggagcgcccg attgtggtcg cgaatggcaa
     3361 agcgcggatt ggtcgtccac cggagcaggt actggaaatc gtgggttaag atgtgcccca
     3421 tcgctagttg aagcacattg ccggatgcga cgcttgccat gtcttatccg gcctacgaat
     3481 actatggatt aacttcgcgt agttcggata aggcgttcgc gccgcatccg acaataaaca
     3541 ccttatctac aacttcagaa tttctttcac aaacggaatg gtcagcttac gttgcgcggt
     3601 aatcgacgca cgatccaact gatccaacgt cataaatagc gtgcgcattt ctctgtcgag
     3661 ccgcttcagc aagaaacgcc ccacatcttc cggcagttca aaaccacgca aacgcgcgcg
     3721 taactgtagc gcctgcaact tatcttcatc agaaagtggc tgcaatttgt agatctgccc
     3781 ccagtcgagt cgcgacgcga gatccggtaa tcccagattc aactgccgcg gtggacgatc
     3841 gccggtgatc aacaatcgtg ttttgcccga ttccagaatt cgattgtaga gatcgaaaat
     3901 cgccatctcc cacaactcat cgcctgcaat acactcaatg ttgtcgatac agaccagcga
     3961 caaatgctcc ataccgtcga gcacttccgg aacaaaccag gtgcgtttat ccagcgggac
     4021 atagcccacc gcatcgccac gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct
     4081 gcgccccgcg ccttcgcgtg cccagagata gatgtaaccg ctatgttcct gacgcagcac
     4141 gttttgcagc gcagccagta aagaggagtt atcccccggc cagaaacttg caaaggtttc
     4201 gtcgtcagga agataaagtg gcaaagagag ctgtgccggt gtgttcagag atacctcaac
     4261 caggatttca caaaatcgcg agaagtttac cacagaatcc catgatgttt gaaccgggca
     4321 gcaacactgc ccggtcggta cattatttgt ctgttatatc cgcgtcttct gcgtccagca
     4381 ccacttcttc agggcgcagc acactaatca gtttgaagat caaacttaag ccgataccga
     4441 cgatggtcgc cagcgccata cctttcagct ccgccgcgcc aatgtttacc ttcgcgccac
     4501 tgacaccgat gatcaaaatc acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca
     4561 cttttgattc gatcaaaaca cgaataccgg aagcaccgat gacaccataa agcagcagcg
     4621 aaacgccgcc catcaccggc aatgggatca tctggatagc ggcagccagt ttaccgacgc
     4681 aggaaagcag gatagcgaaa atcgccgccc caccgataac ccaggtactg taaacacggg
     4741 tgatcgccat cacgccaatg ttttctccgt aagtggtatt tggcgtagag ccaaagaagc
     4801 cggagatcac agtcgacagg ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca
     4861 gcagatcttt tttgacgata ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa
     4921 ccactaacgc cgctggcaga atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga
     4981 gggttggcag cgcaaaccag tgagcattaa taatcggcgt ggtatcgaca attcccattg
     5041 cgaaagagag cgcgtacccc accagcacgc caattaaaat cgggataatt gccaggaaac
     5101 cacggaacag cacggaacct aaaaccgtga ccgccagggt ggtaatagag atgatgatgg
     5161 ttttggagtc tggcgtttgc ccttcagccg ggagtaaacc cgccataccg gcagctacgc
     5221 ccgccagctc cagaccgatg acggcaacga ttgcgcccat tgccgcaggt ggaaacagca
     5281 cgtccagcca gccggtcccc gctttcttca cgataaaaga aaccaggcag aacagcacgc
     5341 cgcacataat aaagccgccc agcgcgactt cataccctaa cggcaacagt aacaataccg
     5401 gtgaaataaa ggcaaagctg gaaccaagat aagccggaat tttcccttta cagatgaaga
     5461 gatacagcag cgttccaata ccgttaaata acagtacagt cgccgggtta atatgaaata
     5521 agacgggcac caggacggtt gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga
     5581 ttgtctgtaa aagtggcggt ctttcactca ccccgatagc acggcgcgtc atagtattat
     5641 cctctgtatt atgtgttata ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct
     5701 ttaattattt ttattcttta tttcgtacca aagattttgt caccggcatc gccgaggccc
     5761 ggaataatgt atccgtgctc gttcagtccc tggtcaatcg atgcggtata cagttcgacg
     5821 tccgggtgcg ctttttccag cgcagcgata ccttctggcg cagctaccag caccagaact
     5881 ttgatgctgc tgcagcccgc ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg
     5941 gttgccagca ttgggtcaac gatcagcgcc atacgctcat cgatgttaga aaccagtttc
     6001 tggaagtacg gtaccggctc cagcgtttct tcattacggt acataccgac aacgctgatg
     6061 cgcgcgctcg gaacattttc cagcacaccg tccatcatcc ccagaccggc acgcagaatt
     6121 ggcacaacgg taattttctt acctttgatc tggtcgattt ctaccgggcc gttccagcct
     6181 tcgatagtta ctttttccgt ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc
     6241 acttcggaag cgagttcgcg aaagcgcttg gtgctgatat cttgctcacg catcagtccc
     6301 agcttgtgtt tgacgagtgg gtgtttgact tccacgatct tcatactctt tctcctttga
     6361 ggggcagcca caaaaaaaat cgacggatta tacctccttt cttcaaggcg gcaatattct
     6421 tttcgttgac tttagtcaaa atgataacgg tttgagataa agttatttta tattcaaatg
     6481 gttatgaaag aagattattc catccgaaaa ctaacctttg ccctggcaca agtcttcttt
     6541 cgccgcgcgt ctggggaaaa gacgtgcaaa aaggttgtgc aaagcagtct cgaaaacgtt
     6601 tgctttccct gttagaattg cgccgaattt tatttttcta ccgcaagtaa cgcgtgggga
     6661 cccaagcagt gaccgataaa acctctctta gctacaaaga tgccggtgtt gatattgacg
     6721 cgggtaatgc tctggttgga agaatcaaag gcgtagtgaa gaaaacgcgt cgtccggaag
     6781 tgatgggcgg tctgggcggc ttcggtgcgc tgtgtgcatt gccgcaaaaa tatcgtgaac
     6841 ccgtgctggt ttccggcact gacggcgtag gtaccaagct gcgtctggca atggacttaa
     6901 aacgtcacga caccattggt attgatctgg tcgccatgtg cgttaatgac ctggtggtgc
     6961 aaggtgcaga accgctgttt ttcctcgact attacgcaac cggaaaactg gatgttgata
     7021 ccgcttcagc ggtgatcagc ggtatcgcgg aaggatgtct gcaatcgggc tgttcactgg
     7081 tgggtggcga aacggcagaa atgccgggga tgtatcacgg tgaggattac gatgtcgcgg
     7141 gtttctgcgt tggcgtggta gaaaaatcag aaatcatcga cggctctaaa gtcagcgacg
     7201 gcgatgtgct gattgcactc ggttccagcg gtccgcactc gaacggctat tcgctggtgc
     7261 gcaaaattct tgaagtcagc ggttgtgatc cgcaaaccac cgaacttgat ggtaagccat
     7321 tagccgatca tctgctggca ccgacccgca tttacgtgaa gtcagtgctg gagttgattg
     7381 aaaaggtcga tgtgcatgcc attgcgcacc tgaccggcgg cggcttctgg gaaaacattc
     7441 cgcgcgtatt gccagataat actcaggcag tgattgatga atcttcctgg cagtggccgg
     7501 aagtgttcaa ctggctgcaa acggctggta acgttgagcg ccatgaaatg tatcgcacct
     7561 tcaactgcgg cgtcgggatg attatcgccc tgcctgctcc ggaagtggac aaagccctcg
     7621 ccctgctaaa tgccaacggt gaaaacgcgt ggaaaatcgg tatcatcaaa gcctctgatt
     7681 ccgaacaacg cgtggttatc gaataatgaa tattgtggtg cttatttccg gcaacggaag
     7741 taatttacag gcaattattg acgcctgtaa aaccaacaaa attaaaggca ccatacgggc
     7801 agttttcagc aataaggccg acgcgttcgg ccttgaacgc gcccgccagg cgggtattgc
     7861 aacgcatacg ctcatcgcca gcgcgtttga cagtcgtgaa gcctatgacc gggagttgat
     7921 tcatgaaatc gacatgtacg cacccgatgt ggtcgtgctg gctggtttta tgcgcattct
     7981 cagcccggcg tttgtctccc actatgccgg gcgtttgctg aacattcacc cttctctgct
     8041 gccgaaatat cccggattac acacccatcg tcaggcgctg gaaaatggtg atgaagagca
     8101 cggtacatcg gtgcatttcg tcaccgatga actggacggt ggcccggtta ttttacaggc
     8161 gaaagtcccg gtatttgctg gtgatacgga agatgacatc accgcccgcg tgcaaaccca
     8221 ggaacacgct atttatccac tggtgattag ctggtttgcc gatggtcgtc tgaaaatgca
     8281 cgaaaacgcc gcgtggctgg atggtcaacg tctgccgccg cagggctacg ctgccgacga
     8341 gtaatgcccc gtagttaaag cgccagctct gccgctggcg tttttcaatt cacctgtaaa
     8401 tcgcaagctc cagcagtttt tttccccctt ttctggcata gttggacatc tgccaatatt
     8461 gctcgccata atatccaggc agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt
     8521 caggaaaagc tatacatcga aaaagagctc agttggttat cgttcaatga acgcgtgctt
     8581 caggaagcgg cggacaaatc taacccgctg attgaaagga tgcgtttcct ggggatctat
     8641 tccaataacc ttgatgagtt ctataaagtc cgcttcgctg aactgaagcg acgcatcatt
     8701 attagcgaag aacaaggctc caactctcat tcccgccatt tactgggcaa aattcagtcc
     8761 cgggtgctga aagccgatca ggaattcgac ggcctctaca acgagctgct gctggagatg
     8821 gcgcgtaacc agatcttcct gattaatgaa cgccagctct ccgtcaatca acaaaactgg
     8881 ctgcgtcatt attttaagca gtatctgcgt cagcacatta cgccgatttt aatcaatcct
     8941 gacactgact tagtgcagtt cctgaaagat gattacacct atctggcggt ggaaattatc
     9001 cgtggcgata ccatccgtta cgcgctgctg gagatcccat cagataaagt gccgcgcttt
     9061 gtgaatttac cgccagaagc accgcgtcga cgcaagccga tgattcttct ggataacatt
     9121 ctgcgttact gccttgatga tattttcaaa ggcttctttg attatgacgc gctgaatgcc
     9181 tattcaatga agatgacccg cgatgccgaa tacgatttag tgcatgagat ggaagccagc
     9241 ctgatggagt tgatgtcttc cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt
     9301 gtttatcagc gcgatatgcc caatgcgctg gttgaagtgt tacgcgaaaa actgactatt
     9361 tcccgctacg actccatcgt ccccggcggt cgttatcata attttaaaga ctttattaat
     9421 ttccccaatg tcggcaaagc caatctggtg aacaaaccac tgccgcgttt acgccatatt
     9481 tggtttgata aagcccagtt ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg
     9541 ctctattatc cttatcacac ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc
     9601 gacccgagcg tactggcgat taaaattaac atttaccgtg tggcaaaaga ttcacgtatc
     9661 atcgactcga tgatccacgc tgcgcacaac ggcaagaaag tgaccgtggt ggttgagtta
     9721 caggcgcgtt tcgacgaaga agccaacatt cactgggcga agcgcctgac cgaagcaggc
     9781 gtgcacgtta tcttctctgc gccggggctg aaaattcacg ccaaactgtt cctgatttca
     9841 cgtaaagaaa acggtgaagt ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa
     9901 aaaaccgcgc gtctttatac tgactattcg ttgctgaccg cagatgcgcg tatcaccaac
     9961 gaagtacggc gggtatttaa ctttattgaa aacccatacc gcccggtgac atttgattat
    10021 ttaatggtgt caccgcaaaa ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc
    10081 gccaacgcgc agcaagggct gcccagtggt atcaccctga agctaaataa ccttgtcgat
    10141 aaaggcctgg ttgatcgtct gtatgtggcc tccagctccg gcgtaccggt taatctgctg
    10201 gttcgcggaa tgtgttcgct gatccccaat ctggaaggca ttagcgacaa cattcgtgcc
    10261 atcagtattg ttgaccgtta ccttgaacat gaccgggttt atatttttga aaatggcggc
    10321 gataaaaagg tctacctttc ttccgccgac tggatgacgc gcaatattga ttatcgtatt
    10381 gaagtggcga caccgctgct cgatccgcgc ctgaagcagc gggtgctgga catcatcgac
    10441 atattgttca gcgatacggt caaagctcgt tatatcgata aagaactcag taatcgctac
    10501 gttccccgcg gcaatcgccg caaagtacgg gcgcagttgg cgatttatga ctacatcaaa
    10561 tcactcgaac aacctgaata accctatgcc aatacacgat aaatcccctc gtccgcagga
    10621 gtttgctgcg gtcgatcttg gttcaaacag ttttcacatg gtcatagccc gtgtggtaga
    10681 tggtgccatg cagattattg gccgcctgaa acagcgggtg catctggcgg acggcctggg
    10741 gccagataat atgttgagtg aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt
    10801 tgccgaacgg ctacaagggt tttctcctgc cagcgtctgt atagtcggca cccatacgct
    10861 gcgtcaggcg ctgaacgcca ctgactttct gaaacgcgcg gaaaaggtca ttccctaccc
    10921 gattgaaatt atttccggta atgaagaagc ccgtctgatt tttatgggcg tggaacatac
    10981 ccaaccggag aaaggtcgca aactggttat tgatattggc ggcggatcta cggaactggt
    11041 gattggtgaa aatttcgaac cgattctcgt tgaaagccgc cggatgggtt gtgtcagctt
    11101 tgcccagctt tacttccctg gcggggtcat caataaagag aattttcagc gcgctcgtat
    11161 ggcggcagca caaaaactgg aaactttaac ctggcaattc cgtattcagg gctggaacgt
    11221 ggcaatgggc gcttccggta ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga
    11281 gaaagacggg ataattaccc cggaacgtct ggaaaaactg gtaaaagaag ttttacgtca
    11341 ccgtaatttc gcatcgctga gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt
    11401 tcctggactg gcgattttat gcggtgtgtt tgatgcttta gccatccgtg aactgcgcct
    11461 ttctgacggg gcgttacgcg aaggcgtact gtacgaaatg gaaggacgtt tccgtcatca
    11521 ggatgtgcgt agtcgcaccg ccagcagcct cgccaaccag tatcacatcg acagcgaaca
    11581 ggcccgacgg gtgctggata ccactatgca aatgtacgaa cagtggcggg aacagcaacc
    11641 gaagctggcg catccgcaac tggaggcgct actgcgatgg gccgccatgc tgcatgaggt
    11701 cgggttgaat atcaaccaca gcggtttgca tcgccactcc gcttatattc tgcaaaacag
    11761 tgacttgccg ggttttaatc aggaacagca gctgatgatg gcgacactgg tgcgctatca
    11821 ccgtaaagcg attaaactcg acgatctgcc gcgctttacc ttgtttaaga agaaacagtt
    11881 cctgccactg attcagctat tgcgccttgg cgtattactc aacaatcaac gtcaggcaac
    11941 caccacaccg ccaacattga cactgattac tgatgacagt cactggacac tgcgtttccc
    12001 gcatgactgg tttagtcaga atgcgttggt actgcttgat ctggaaaagg agcaagaata
    12061 ctgggaaggc gtggctggct ggcggttgaa aattgaagaa gaaagtacac ctgaaatcgc
    12121 cgcttaatgc tttgcgggcc gacgagaaat gtcggcccgc attattcagg cactttcgcg
    12181 aatgggttcg atttcattca acgtatcaat taacggctgc ggcttaccaa taagataacc
    12241 ctgcatataa tcgatcccca aagagagcac cgcctcgcgg atctcttcgt tttcaacgta
    12301 ctctgccact acccgcattt tcttcattcg cgccagatgg caaatcgacg ccacaatctg
    12361 ataatccaga ctatttgaca caatattgcg gataaagctg ccgtcaattt taagcagatc
    12421 ggcattcaca tttttaagcc gtgcatagct ggcgtagccg gtgccgaaat catcaatcgc
    12481 aatctggcag cctaattcct gaagatgctg caaggtgatt tgcgcctgct taacattggt
    12541 cagagcatta ctttcggtga cttcaaaaat aagttgccac gcttcaatct gatatttagc
    12601 cagcaactga ctgacttcaa caggaaaacg agcctgacat accgaggttg gagacagatt
    12661 aatagcaaaa cggtgagcgg gcatcttcgc tctgttttca gccataaatt gcagcgtatg
    12721 ctcaatgacc cacatgtcga tactcgacga taaaccaaat tcgtgcgcga ccggcaagaa
    12781 gctatcgggg acgatcagtt catcattctc acctttcatg cgcagaagaa tttcatggta
    12841 aacatcacca cgcataccgg taatcggctg ggccatcagg aaaaaatggt tgtgttccag
    12901 cgcctgctgt agtcgattca tcatcgcgac tttatctttc aattcgcgtt gcaaatacat
    12961 tgccccgcga cgctgcatat tttccggggc gttggtcacg atggaaagtt cggcgaccgt
    13021 atttagctct cccagcagca ggtagatatg attcactggc gagcgcacat agcagtaact
    13081 gacgccaatc tgcggttgca tcggcatccc atcccaaaag aaacgaaatt gcttgagatg
    13141 actatccagt gcggtaatgc gctcctggtg cgattctgta ttcagtcgca gcgcgagatc
    13201 gttacccgaa agctgataaa catcttcacc cggttccagc aagggtgaca gccagtgaga
    13261 aagtttttgc ttgtattgaa tccgcagcat gatgccatag ttcttaacca gcatttccat
    13321 gccagggatg cgtaaataac aaagcgcaga ccagggagca tcacgcaacg cgcgattcag
    13381 ggcgcgaaca tttggcagat gaaccaccgg gtccatatac gcaagccgct gaatgcgtct
    13441 gactaccgct ctctgacggg ttgccagtac agccatataa ttgacaataa aagagaatac
    13501 cagataactg gaggaggtta tggtcagctg cgtggtatag ccaggataaa tgggaatgta
    13561 attttgatag ctgtggatgc tgatcatcaa cacgaccgcc cagagcagcg aaatcagctt
    13621 ataaccatag cgcatcgctc cccacatcat caggggcagc aataatgaca aggtataatt
    13681 ggtgctaaaa attgtgcttt tttcatttaa cggcatgcat aacagcaaca ataaagcacc
    13741 taatgccagt agccagagcg cgaactcttt tttggtgact ttggcatcaa cctgctgttt
    13801 taattgcgaa taatagctac gtaaataaaa tggatttcgc actacccgaa tgatgaagta
    13861 gcacagcggg acaccgatca gattacccac cagtaaggcc tgataattga ttaaagtccc
    13921 gaggttaaaa ggcatgacac cgaccagatt ttctctgctc gccagtaatc ctacaaatgc
    13981 agcaaactgg aaaagtatca gaaacagcgt tgcaggaaac acaatctgcc agaatatacg
    14041 ctgggaaatt aaacgggtat caccatgtga aacgttgtta cgccggggag taaagaccct
    14101 gtaaccgccc caacagagaa tgataatgat aataaaatta gccgttaatg atagcgtttc
    14161 gtaaaaaccc agctctgcat atttgcggac aaaaatccct aaggcgatcc ctggcaatgc
    14221 cgcccagctg aaaaacatca tcatgctgat catcagtgcc agaggcagat aaaagagaaa
    14281 aacctcaccg gaagaaatat gcgcgaaagt attaatgtgg gcgaaaatgg gcaagattaa
    14341 agaaggcagg aacagcggaa gcccccacca tttatcacgc atttttatat aagttgcatt
    14401 cagtttcata gatgctcagc agaatccccc acatcctgaa ggaggtgtat tcagacaggc
    14461 atcccacctg acttcgaatg atgattattc atcactatag agagcattga ttctaagtgt
    14521 catatgaaag ttccaattga tatatatcaa acaaaataac cctgattaat gaattattac
    14581 gtttatcatg ttaattcatc attattacat catcattgta aataattaaa ttaacttcca
    14641 taacattaaa atatgtatcc actgacgttt ttttacataa cgaagaattg accattttgt
    14701 cctgttgtgc cttaatgtaa gtaccgtcca cagcgtggga catacttcaa ggaacctttt
    14761 gtgagtcagg caaccagtat gcgaaaacga caccgattta acagtcgcat gacccgtatc
    14821 gtactgctca tcagctttat cttcttcttt ggccgtttta tctactcgtc cgtcggtgcc
    14881 tggcagcacc atcagagcaa aaaagaagct cagcaatcca cactctccgt cgaatcaccg
    14941 gtacaacgtt agcggttacc ttcttcactt tcacagaaca taacggcact tcgctgtcgg
    15001 atgcttttgc tctttgggat tatcaaagcg gcagatattc tttcatctta aattttacgt
    15061 ctttgtcctg actgatgttt atcctgtttg gctgcggaat aaatataaaa ttaatatata
    15121 tgttgtaatg atatattttt ataaattatt ccctgcgtga attttaataa atttaatcta
    15181 tccctttata cgcaatacat ttactttcct cttttgatga tcttaaatgt cttatttttc
    15241 gtaatgtgta taacaaggaa tagtgatgaa atttaaaaaa tgtcttctgc ctgtggcaat
    15301 gttagcgtca ttcactctgg caggatgcca gtcaaatgct gacgatcatg ccgccgatgt
    15361 ttatcaaacc gatcaactga ataccaaaca agaaactaaa accgttaata ttatttccat
    15421 tcttcccgca aaagttgccg tagacaactc ccaaaataaa cggaacgcac aagccttcgg
    15481 cgcgcttatt ggcgcagtcg ctggcggtgt tatcggccac aacgtcggtt ctggcagcaa
    15541 ttccggaacg acggcagggg cagttggcgg cggagctgta ggcgcggcag cgggttctat
    15601 ggtgaatgat aaaaccttag tggaaggtgt ttctttaaca tataaggaag gcaccaaagt
    15661 gtatacctcc acccaggtgg gtaaagagtg ccagtttacg acaggtttag ccgttgttat
    15721 taccacaacg tataacgaaa cgcgtattca gccaaatact aaatgtcctg aaaagagcta
    15781 ataatcagga ggagtcatga agaaagtttt tctttgcgcc atcttagcct ccttaagcta
    15841 tccggctatc gcctcatcat tgcaggatca actctctgct gtcgcagaag cggaacagca
    15901 aggtaaaaat gaagagcaaa ggcagcatga cgaatgggtc gcggagcgca acagggaaat
    15961 ccagcaagag aagcaacgtc gcgcaaatgc ccaggccgcc gctaacaaaa gagcggcaac
    16021 ggcagcggca aataagaaag ctcgtcagga taaactggac gccgaagcca ctgcggacaa
    16081 aaaacgcgat caaagttatg aagatgagct acgcagctta gagattcaga aacaaaaact
    16141 gacgctggcg aaagaagaag cccgcgttaa gcgagaaaac gaatttatcg atcaggaact
    16201 gaagcacaaa gctgcgcaaa ccgatgtggt gcaatctgaa gctgacgcca acagaaatat
    16261 gactgaaggc ggtcgcgatc tgatgaaaag cgtgggcaaa gcagaagaga acaaatcgga
    16321 cagctggttt aattaatcga tgttagtaac ttcaagccta tgattcttga agataaaaaa
    16381 ccctctgtag taacagaggg ttttgttcat tcatagtgca gggattaaaa tcattcccac
    16441 tcaatggtag ctggcggctt gccgctgatg tcatacacca cgcgggaaat accgttcact
    16501 tcattgataa tgcggttgga aacgcgaccg aggaaatcgt acggcagacg cgcccagtgt
    16561 gcggtcataa agtcgatggt ttcgacagca cgcagagaga caacccagtc atacttacga
    16621 ccatcgccca ttacgccaac ggaacgtacc ggcaggaaca cagtgaacgc ctggctgact
    16681 ttgtcgtaca ggtccgcttt acgcagttct tcaatgaaga tggcgtcagc acggcgcagc
    16741 aggtcacagt actctttctt cacttcaccc agcacacgaa cgccaaggcc tggtcccggg
    16801 aatgggtgac ggtacaacat gtcgtacggc aggcccagct ccagaccaat cttacgcact
    16861 tcgtctttga acagctcttt cagcggttca accaggccca tcttcatctc tttcggcagg
    16921 ccgcccacgt tgtggtgaga tttgatgacg tgtgctttac cggttgcaga cgccgcagat
    16981 tcgataacgt cagggtagat ggtgccctgc gccagccact tcacgtcttc cagtttcagc
    17041 gcttcttcat cgaatacttc aacgaaaacg cgaccgatga ttttacgttt tgcttccgga
    17101 tcgttttcgc cagccagcgc tgacaggaag cgatcttctg ccggaacgtg aacaatgttc
    17161 agaccaaagt gatcgccaaa catatccaga acctgctctg cttcgttgag acgcagcagg
    17221 ccgttgtcga cgaatacgca agtcaggttt ttaccgatag cgcggtgcag tagcattgcg
    17281 gttacggagg aatccacgcc accagagagg ccgaggatga ctttatcgtc gcctacctgc
    17341 tcacggatgc gggctacagc atcgtcgata attttcgctg gcgtccacag ggcttcacac
    17401 tggcagatat cacgcacaaa acgctccagc atgcgcatac cctggcgggt gtgagtcact
    17461 tccgggtgga actgtacgcc atagaagcgt ttttcttcgt tagccataat ggcaaacggg
    17521 cagctttcgg tgctggctac ggtgacgaag tcggacggga tagcggtaac tttatcgccg
    17581 tggctcatcc agacatcgag cagcggttta ccgtctgcgg tcagcgcatc ttcgataccg
    17641 cgaaccagtg cactgtcgtt tacgacttca acctgcgcgt agccaaattc acgttcgtta
    17701 gaggcttcaa cgtgaccgcc caactgcatt gccatggtct gcatgccata gcaaacgccg
    17761 aataccggta cgcctgcttc aaagacatac tgcggcgcac gcgggctgtt ttcttcagta
    17821 gtactttccg ggccgccgga aagaataatg ccgcttggat tgaagtcacg aatttgtgct
    17881 tctgtcacat cccacgccca cagttcgcag taaacaccca gctcacgcac gcggcgcgca
    17941 accagttgag tgtactgaga accgaagtct agaatgagga tgcgatgctt atgaatgttt
    18001 tccgtcattg acgcttattc cgaggcaagt gaaacagata atataaatcg cccgacatga
    18061 agtcgggcga agagaatcag gagcccagac ggtagttcgg ggactcttta gtaatggtca
    18121 cgtcgtgaac gtggctttcc tgaatgcccg caccgctgat acgtacaaac tccgctttag
    18181 tacgcagttc gtcgatagta ccacagccgg tcagacccat acaggagcgc aggccgccca
    18241 tctgctggtg aatgatctct ttcaggcgac ctttataagc tacgcgacct tcgatacctt
    18301 ccggcaccag tttgtcggca gcgttatcgc tctggaaata acggtcagag gaacctttgg
    18361 acatcgcgcc cagggaaccc ataccacggt aagatttgta agaacggccc tggtagagtt
    18421 cgatttcacc cggagattct tcagtacccg ccagcatgga acctaccatc accgcgcttg
    18481 cgccagcggc gatagctttg gcgatgtcgc cggagaagcg aataccacca tctgcgataa
    18541 ccggaatacc ggtgccttcc agggcttcaa ctgcgtcagc aacggcagtg atctgcggaa
    18601 cacctacgcc agtaacgata cgagtcgtac agatagaacc agggccgata ccgactttaa
    18661 ccgcgctgca accagcttct gccaaagcgc gtgcacctgc agctgttgcc acgttgccgc
    18721 cgatgatttg cagatccgga tatttagcac gggtttcacg gatacgctgc aaaacgcctt
    18781 ctgagtgacc gtgggaggag tcgatcagca gaacgtcaac gccagcggca accagcgcgt
    18841 caacacgctc ttcgttaccc gcacctgcgc caactgcagc accaacacgc agacggcctt
    18901 gctcgtcttt acaggcgttc ggtttacgtt ccgctttctg gaagtctttc acggtgatca
    18961 tgccgatcag gtggaattcg tcatcaacca ccagcgcttt ttcaacgcgt ttttcgtgca
    19021 tttttgccag caccacttca cgggcttcac cttcacgcac ggtgaccaga cgctctttcg
    19081 gcgtcatgta aacgctaacc ggctggttca ggtcggtaac aaaacgcacg tcacgaccgg
    19141 tgataatacc caccagttcg ttttcttcgg taacgaccgg atagcccgca aaaccgttac
    19201 gctcggtcag ttctttcact tcgcgcagcg tcgtggttgg cagaacagtc tgcggatcag
    19261 tcaccacacc agattcgtgt tttttcacac ggcgaacttc ttctgcctgg cgttcaatgg
    19321 acatgttttt gtggataaag ccgataccgc cttcctgagc cagagcaata gccaggcgcg
    19381 cttccgttac ggtatccatt gctgcggaaa gcatagggat attcagacga atagttttcg
    19441 tcagctgggt gctgaggtca gcagtattcg gcagaacggt agaatgagca ggaacgagga
    19501 gaacgtcgtc aaacgtcaga gcttctttag cgatacgtag catgggcaat atctcgacca
    19561 gagtggttaa taaatattgc cgcggcatta tacagagcgt aaccgattgc atctacccct
    19621 ttttgcaaaa aatgcttgct atccccgaag ggcgggttac tatcggctga ataacctgct
    19681 gatttagaat ttgatctcgc tcacatgtta ccttctcaat cccctgcaat ttttaccgtt
    19741 agtcgcctga atcaaacggt tcgtctgctg cttgagcatg agatgggaca ggtttggatc
    19801 agcggcgaaa tctctaattt cacacaaccg gcttccggtc actggtactt tacactcaaa
    19861 gacgacaccg cccaggtacg ctgcgcgatg ttccgcaaca gcaaccgccg ggtgaccttc
    19921 cgtccacagc atggacaaca agttttagtt cgcgccaata ttacgctcta cgagccgcgc
    19981 ggtgactacc agataatcgt tgagagtatg cagccggccg gtgaagggct gctgcaacag
    20041 aagtacgaac agctcaaagc gaagttgcag gctgaaggtt tgttcgatca gcaatacaaa
    20101 aaaccacttc cctcccctgc gcattgcgtt ggtgtgatca cctcaaaaac cggtgctgcg
    20161 ctacatgata ttttgcatgt gttaaaacgt cgcgatcctt ctctaccggt gatcatctac
    20221 cccaccgccg ttcagggcga tgacgcaccg gggcaaattg ttcgcgccat tgaactggcg
    20281 aatcagcgca atgagtgcga cgtgttgatc gttgggcgcg gcggcggttc gctggaagat
    20341 ttatggagtt ttaacgacga acgcgtagcg cgggcgattt ttgccagccg cattccggtc
    20401 gtcagcgccg tcgggcatga gacggatgtg accattgccg attttgttgc cgatctgcgt
    20461 gcgccaacgc cgtctgccgc cgctgaagta gtgagccgta atcagcaaga gttactgcgc
    20521 caggtgcaat cgacccgtca acggctggag atggcgatgg attattatct cgccaaccgc
    20581 acgcgtcgct ttacgcagat ccatcaccga ttacagcaac agcatccgca gctccggctg
    20641 gcacgccagc aaaccatgct tgagcgcctg caaaagcgga tgagctttgc gctggaaaat
    20701 caacttaagc gtaccgggca acagcagcag cggttaacac agcggctgaa tcagcaaaat
    20761 ccacagccga agattcatcg cgcgcaaacg cgcattcagc aactggaata tcgtttagca
    20821 gaaaccctgc gcgtacagct tagcgccacg cgtgaacgtt tcggtaatgc agtaacgcac
    20881 ctcgaagccg taagcccact gtcaacgctg gcgcgtggat acagcgttac tactgctact
    20941 gacggcaatg tactgaaaaa agtgaagcaa gttaaagcgg gtgaaatgct aaccacacgt
    21001 ctggaagacg gctggataga aagtgaagtt aaaaacatcc agccagtaaa aaaatcgcgt
    21061 aaaaaggtgc attaagccag cacgaattca acgcgttttt tcgatatcaa gccatgcccg
    21121 tgctgacaga agtaatctac tgcaccgcag gcttttaaca cctgcaacgg ttgatggcag
    21181 tctggacaaa gagctttcat ttcaataatt tccccgcagg atgggcaacg cgcatggccg
    21241 ttatcctgat caagcacatg ctgacattgt ggacaatgca gttccatatg aattcctccg
    21301 gtagccatcc ctctacattc atagagggaa tggcagataa aatacttacg gataattatt
    21361 tatttttctt gatgtgcttc atcagacgct tacgtttacg catctgggtt ggcgtcaggg
    21421 tgttacgctt gttcgcatac gggttttccc cttctttgaa ctgaatacga atcggcgatc
    21481 ccattacgtc cagcgatttg cggaagtagt tcatcaagta gcgcttgtag gaatcaggca
    21541 ggtctttcac ctgattaccg tgaatcacca caatcggcgg gttataacca ccagcgtggg
    21601 catatttcag cttcacacga cgaccgcgta ccagcggcgg ttggtgatct tcaacagcca
    21661 tcgtcatgat gcgcgtcagc atagaggtcc ccacacgacg ggtggagctg tcatacgctt
    21721 cacgtactga ttcaaacaag ttaccaacac cactgccgtg caaggcagag ataaagtgca
    21781 cacgagcaaa atcgataaag cccagacgga agtccagcgt ttctttcacc tgctctttca
    21841 cttcctggct caggccatcc cacttattca ccacaatgac aagtgagcgc ccactattga
    21901 gaataaagcc cagcagcgag agatcctgat cggaaatacc ttcgcgcgca tcaatcacta
    21961 acatcaccac gttggtatct tcaattgcct gcaacgtttt gattacggag aatttctcta
    22021 cagcatcggt gattttgccg cgtttacgta cgccagcggt gtcaatgagc acatactcac
    22081 gtccatcgcg ttccattgga atgtagatac tgtcacgcgt cgtgccaggc atgtcataaa
    22141 caacaacgcg ctcttcacca agaatacggt tagtgagtgt agacttacct acgttcggac
    22201 gacccacaat cgccagtttg atcggcagac tttgcgggtc gaagtcgtct tcctcttctt
    22261 cttcgccgtt ctcttccgct tcaaattgcg cccagtattc agcgtcttcg tcgacttcct
    22321 cttgcggtgc gagatcttcc atccacggca gcagcacacg ctccagcaga cttaatacgc
    22381 cacgaccgtg agatgcggcg atcgggtaga tttcacctaa accaagcgag tagaaatcaa
    22441 ccactgcctg atcgggatcc agaccgtcag ttttgtttgc caccaggaag gtcggttttt
    22501 cacgagagcg cagatgtttg gcaatcgctt catctgccgg catcaagccc gcgcgcgcat
    22561 ccaccataaa cagtacgacg tccgcttctt caatcgccag cagcgactgt tccgccatgc
    22621 gggtttctac accgtcttct gtgccatcaa tcccgccggt atcaatacag ataaactcac
    22681 ggccttcaat ttccgcacga ccgtacttac ggtcacgagt cagacccggg aaatccgcaa
    22741 ccagcgcatc tcgggtgcga gttagacggt taaataacgt ggattttcct acgttagggc
    22801 gcccgacaag cgcgaccaca ggtaccatgt ttaaagcctc atttttataa atcatcagac
    22861 aacgcacgct atattcgcgt cgttgttaaa aacaggaaaa cggcccctgt ccaggagccg
    22921 ttttcaaagt gaacgacaga gacgattaac gtgtaataga gtacacggtt ccgtcttttg
    22981 cctggatcag cagtttgccg tcagcggcaa ccggttcagt ctggaaaccg gaactatcaa
    23041 ctttttgctg ggcaacgaaa cgaccatctt cgacgttaat ccagtgcaga taaccttcac
    23101 tgtcacctac caccaggttg ccattataca gcaccggcga agtcagcagg cgatgcagca
    23161 gatcgctttg tgtccacagc gtaacgccgc catcaatggt caacgccatc acccggtcat
    23221 tttgatcgac cagatagatg cgattgccgt cgacgatgaa atcattcacc gaacccagtt
    23281 cgcgtttcca cataatctga ccactgcgca gatcaagcgc cgtcaggtta ccattatagg
    23341 ccagagcgaa aacaacgccg ttaacaacga cgggagtcgt gtcaacatcg ctcagacggt
    23401 caatttcggt agaaccggtc gcctgggaaa tacgctgctg ccaaatcatc tggccctgtt
    23461 ccatcagcac tgcgctgacg cgaccattat cgccccccac gacggccgca ccaaaagccg
    23521 ttgccggcgc agactcgcca cgcaaagaga gcgaaggcat atcgaggtta actgtccatt
    23581 tgacagcgcc gtcagcttcg ttcagcgctt gtaactgacc gttactagtg tggattaaca
    23641 ccagaccgtc gctgaccacc gggcgagaaa gtgcttcacc cgcgacttta gtttgccatg
    23701 ccacagtacc atcgctggta ttcagcgcgt aaacctgcgc cttttcgctg ccaatgtaga
    23761 catgcccacc agacacggtc acaccgccag aaagtaatgc aggctcttta gagaaccagc
    23821 catctttctc ggccaggctg acagaccaga tttctttgcc atcatccgca ttcagcgctt
    23881 ttaccaaacc agcgcggtcc gctgcataga caacgttgtc cgccagtgcc ggatgaagat
    23941 tggaatagaa gttgccaatg ccgctaccaa cggaagtgct ccacgccgtg gtcggcgtaa
    24001 actggttttc aacggttggc aatggggaca tctttaccac atcttcttcg ctgttaaaca
    24061 gcgaacagcc gcttaaaagg gtaacggaaa gcagtcctgg cagcagtaat ttacgcaatt
    24121 gcatcgggtc cctctcagat ggacaaatta ttaattttca tctgcatcat ttcgctcagt
    24181 gccggagtaa catcgctttt cacgcctgct tcccatgcac tacgcgcacc ttgcttatca
    24241 cctttgctca acaatgcttt accacgcagg tcggcaacaa tggcagccca cccttcacct
    24301 ttgatggtat caagggtttt cagcgcggca tcagcctgct tgagctgtac ctgaacgcga
    24361 gcaagacgca gatttatcac ggctttgaga ttttcatcac tcgtgtctgc cagcccctgt
    24421 tgtaactggg cggcagcttt ctctagttca tttttgtcaa caaattgctg cgcaagttcc
    24481 aaagaagcca gcgcaccata agtattttta ttttcagcag caaatttttc cgccgccggg
    24541 atgctatccg gtttgccttc gctcactgcg gtaaccgcat tttgataggc aagagaagcg
    24601 gagcgtgcag aatcaacctg atggctgttc cagtagcgcc agccaatcag tgcgccaacg
    24661 cccaaaatca ccccaacagc cagtgctttg ccattttcag caaaaaagcg tttaaccgct
    24721 tctacctggt cgttttcgtt ctcgtaaatt tccacgctgt ccttctcctt ccttaaccca
    24781 gtaacgtgcg caaatgcgcg gctacgctat cctgcgcaac tgccgtttgc tcaccagagc
    24841 gcaaatcctt cactactgct gtgccgttag ccacttcaga ctcacccagc accacagcaa
    24901 cgcgggcacc ccatttatca gcacgggcaa actgtttctt aaagttgccg ccgccgtggt
    24961 tggtcatcaa tttcacgccc ggtaattcat cacgcagacg ctcagctaat gccatagccg
    25021 cagattgtgt atcagcacct gaagccacca ggtatatatc gacaacagga tcggctttaa
    25081 attccggatt aacggcctgt actaacaata caagacgttc gaggcccatc gcaaaaccga
    25141 ctgccggtgt tgcacgaccg cccagttgtt ccaccagacc gtcataacga ccgcctgcac
    25201 acacggtgcc ctgggagccg aggctgttag tcacccactc gaaaacggta cggttatagt
    25261 aatccagacc acgcaccaga cgctggttta cggtgtaagc gatccccgcg ctctcaagca
    25321 gtttgcacag accggcaaaa tgctcacgag attcctcgtc cagataatca cctaatgccg
    25381 gagcgtcgtt gagaagcgcc tgcacttccg gattttttga atccagcacg cgcagcgggt
    25441 tagtgtacat gcggcgtttg cagtcttcgt ccagcttttc tttatgctgc tcaaggaatg
    25501 ccaccagcgc atcgcggtaa ttggcgcgtg cttccagcga accaatagag ttcagctcaa
    25561 gagttacgtg ttcgaagata cccagcgcac gccaccagcg ggcagtgagc ataatcagtt
    25621 cagcgtcgat atccggacct tgcagaccga aaacttcgca gcccaactga tggaactgac
    25681 gataacgccc tttctgcgga cgctcgtgac ggaacatcgg cccgatatac cacagacgct
    25741 gttcctgatt gtacagaaga ccatgctcga tgccggcgcg tacacagccc gccgtccctt
    25801 cagggcgcag agtcaggctg tcgccattgc gatcctcaaa ggtgtacatc tctttttcaa
    25861 ccacgtcggt gacttcacca atcgcacgtt tgaatagcgg ggtctgctct acaatcggca
    25921 agcggatttc actgtaaccg tagctgccga gcacgttttt cagtgtgcct tcaatgcgct
    25981 gccagatggc cgtttcgcca ggcaggtaat cgttcatgcc gcgaatggct tgaatgtttt
    26041 ttgccacgtt tattctcttt ctgaatataa aaatgaaccc tcaacgcttc cctcaatgtt
    26101 tcgggagcca tgcgggttca atcatacacg ggaagcgagg cgcttcccat cacgttatta
    26161 tttttcaacc tgctgaacgt caattcgacg cgcttcgtcc agctgactgg ctttcgcacg
    26221 aatgcgtgct tccagctggt cgatcatatc gttgttgtcc agacggtctt tgcgcacgcc
    26281 atcttcatag aggccgcttt tcttgttgcc gccggtgacg ccgagtgtag aaaccagcgc
    26341 ctcacctggg ccattcacca cgcagccgat aatcgaaacg tccatcggag tgatgatatc
    26401 ttccaggcgt tgctccagcg cgttaaccgt accgataaca tcaaattcct gacgcgaaca
    26461 ggtcgggcag gcgatgaagt taatccctcg cgaacggata cgcagcgatt tcaaaatatc
    26521 gaaaccgact ttgatctctt cgaccggatc ggccgccagc gatacgcgca gcgtgtcgcc
    26581 gatgccttcc gacagcagca gacctaaacc aatggcggat tttactgccc cgctgcgtgc
    26641 accaccggct tcggtgatcc ccagatgcag cggctgatcg atctgttttg ccagcaaacg
    26701 ataagactca acagcgagga agacgtcaga cgctttcacg ctgactttga actgatcgaa
    26761 gttcaggcga tcgagatgat caacatgacg catggcggat tccagcaacg cctgcggcgt
    26821 cggttcgcca tacttttctt gcagatcttt ttccagcgat ccggcgttaa cgccaatacg
    26881 gatcggaatg tttttatcgc gcgcacagtc aaccaccatg cgaatacgct cttcattacc
    26941 gatattgcca gggttaatac gcagacaatc gacgccgtat tccgctactt tcagcgcaat
    27001 gcgatagtcg aagtggatgt cagccaccag cggcacgtta acccgctgtt tgatgagttt
    27061 gaacgcttct gccgcgtcca tcgtcggtac ggagacacgg acgatatcag cgccaacgcg
    27121 ttccagcgcc ttgatttgat tgaccgttgc ttcgacatct gtcgtacgcg tattggtcat
    27181 ggactgtacg gcgatgggag caccatcgcc aatcggcaca ttcccaacgt aaatacgtgt
    27241 tgattttcta cgttgaattg gagcctggtt atgcatgaaa aatctcccgc gttacccgtc
    27301 tgttactgcg ccggtgattg ttcggcattg agggtcagac gcgcaacctg gttagttctg
    27361 ataaaacgac tcagatcgac aggtttccct tgatactgga tctgtactgc ggctggcgca
    27421 ccaattttca gtttatacgg tgcctggccg gttaaattca aattaccgtc tttacgctgc
    27481 atcccgctaa acaatttttt accggtagca tcagtgacct ccagccagca atcggcagta
    27541 aagttcatca ccagcgcatt cggatcagcc gccggcgtgg tcacgccagc ctgatcggtt
    27601 ggcaacggcg cagcaccatc tggcgttgtt gctgccgttg gtaccggggt cgcggcggta
    27661 tcaacatttg cctgcgaagg cgaaacaacc gcattctgtt gcggatcaac agctggtgct
    27721 ggcgcagtta cggcaggtgt ttgcgtgttg gttgcggtag tatccacaga cgctggcggc
    27781 gtggacgttg tagccgggtc tgtagttgtc gacgtattta acgggacact ctgcccctgc
    27841 tcgctattac tgctcagttc tgccgaagat tgatcggcca tagtggtgat ctcttcctgc
    27901 tgagctttgt ggtcttgcca ccaccaggca ccgctcaggc cgataaccac aaacaacacc
    27961 agccaggtga aggtcatcag ccagccatca cgttttttgc ggcgtttacc gagggaaaaa
    28021 ctctgcattg gcgcaacttt tgcagcccgt agtggagcct gcttttccag ccctggcagc
    28081 agttcttctt ctggaatatg taccagacgc gcataagagc ggatatatcc gcgcaggaat
    28141 gttgaagcaa gatcggcggg tgccttatct tcttcaatgt cgcgtaccgt ggaaaccttc
    28201 aggcaaagtc gctcggcaac ggcctgctga ctaagtccta gttgttcgcg agcattacgc
    28261 aggcgagcgc cggtagtaag tgcttcattt tggtcgtgcg tggcttcagt attcattcgc
    28321 tgcaggtacg tttaaatgag aattgagatg ccggtgaacc atcatgctca cccacaccgc
    28381 gaaacatccg ttaagttaac cgttatcata cagtataaga ccgtctgtgt gctcgtgaca
    28441 aagcccacat tcactaaggc taacttactg ttgcatcgtt acatactgcc ttaaagtcag
    28501 caaaaacgca ccgttaatat tgaccagaca attgcaactt aatacttcat tcataaaaca
    28561 ttacgccacg gtacataaag taaccgtggc gtaatggcta tcagaccgct ttaatgtcga
    28621 tggcttcacc ctgcatccgt ttacgcaggg tacgtttcgt acggtcgata acatcgcccg
    28681 ccaactgacc acaggcagca tcgatatcat caccacgagt tttacgcaca atagtggtga
    28741 aaccgtagct catcagcact tttgagaaac ggtcgatacg gctgttcgag ctgcgtccat
    28801 acggcgcacc cgggaacggg ttccacggga tcaggttgat cttacacggc gtatctttca
    28861 gcagttccgc cagttggtgc gcgtgttcag tgccgtcgtt aacgtggtca agcatcacgt
    28921 attcaatagt gactcggccc tgattggcgt tggatttctc cagataacgg cgcaccgcag
    28981 caaggaacgt ttcgatattg tactttttgt tgatcggcac aatttcgtca cgaatttcgt
    29041 cgttcggcgc gtgcagggaa attgccagtg caacgtcgat catatcgccc agtttatcca
    29101 gcgccggaac tacaccggaa gtagaaagcg tgacgcgacg tttagacagg ccaaaaccga
    29161 aatcatcaag catgatttcc atcgccggaa cgacgttgtt caggttgagc agcggctcgc
    29221 ccatgcccat catcactacg ttagtgattg gccgctgacc ggtgactttt gctgcgccga
    29281 cgattttcgc cgcccgccac acctggccga taatttccga cacccgcagg ttgcggttaa
    29341 agccctgctg ggcggtggaa cagaatttac actccagcgc acaccccacc tgcgaagaga
    29401 cgcagagcgt ggcacggtcg tcttccggga tatacaccgt ttcgacgcgc tgatcgccaa
    29461 cggcgatcgc ccatttaatg gtgccgtcgg atgaacgctg ttcttcaacc acttccggtg
    29521 cgcggatctc cgccacctct ttcagtttgc cgcgcaacac tttgttgatg tcggtcatct
    29581 catcaaagtt gtcgcagcaa tagtgataca tccacttcat cacctgatcg gcgcggaagg
    29641 gtttttcacc taaatcttta aaaaactccc gcatctgctg acggttgaga tccagcaggt
    29701 tgatttttcc atctttcgtg gtgacgtttt caggtgtgac taattgttca gacatatgct
    29761 attccggcct cgttattaca cgttatggcc cctggagggt tgaaaaaaga aacgccccgg
    29821 tgagcggctg ctcgtccggg ggcgctgcat tgtacaaatt ctggcgcacg gatgccacgt
    29881 ttgcacgcgg catttacgaa attattaacg ggtgcgcggg cacacttcgc cttcgccgaa
    29941 gaaataagcg atttcgcgag cggcagattc gacggaatca gaaccgtggg taccgttttc
    30001 ggtcaggctg tcagcgtaat cagcgcgcag agtaccggcc agtgcgtttg ccggattggt
    30061 cgcgcccagc agatcgcggt gacgctgaac ggcgttttca ccttccagca cggaaaccac
    30121 gatcgggcca gaggtcatga attcaaccag accatcaaag aacggttttc catcgtgttc
    30181 agcataaaag ccacgagcct gttcaacggt caggtgcagc attttggtgc cgacaatttt
    30241 gaaccctgca gcttcaaagc gcgcaaagat attaccaatg acgttttttg ctaccgcgtt
    30301 cggtttgatg atggaaaaag tacgttcaat agccattttt acctctgtaa attgttctgt
    30361 tgttgtctgt accagcgtac gagatggcgc ggattataat gagcaacagg gccgttgact
    30421 attgatgaag gtaacatttt tttaaaataa aatgagtttt agcaacaatc agggacaaaa
    30481 cagactattg catgacaaat ttcactgtcg cgatttgccc cacgtcatcc atcaccagca
    30541 attgataatc acctttatcc gtcaaatgca gggtcacgtt gcgcccgcgt tcagttaacg
    30601 gttcgccatt cagaaaccac cagcgttcac ctgcccctcc acttgattgc aacggcaaag
    30661 tcgcttccgc tgcgcccggt aaacgtttaa taatcgcgcc atcgcgaacg cctgtcagct
    30721 gcagagggag ctgggcatcg tgaccgtatg gcgggcaaat tgtcgaggcc ggtggtaagc
    30781 gcacagcgcg acgttctgat gcgggcagcc agggttccag cggtagcggc cagacattta
    30841 ccatttcttg cttcgcctgc gggcagtcag cggcgacgcg ctgccctttg tcatccagcc
    30901 agatggggaa gcgaatgcca ttaatccctt cctgctctga cagtaacagc gttggcggct
    30961 ggctgccatc cagcagccag gttgccaggc ggcggcgaca gttaccgtca ccttccggca
    31021 aagactgtcc gcccggccag cagataacgc cacgagtgac tgagttcggg cgcgggtctt
    31081 ccggcagatt cgcactgcgc gacagtaaga tattattgac ctgattcaac aatggtacgg
    31141 cactggcaaa gccaaactga ccaacaacgg gcgtgccgtc aggtctgcca gtccagatcc
    31201 caatgacata gcgagcgtta accccaatcg cccaggcgtc acgatagcca tagctggtgc
    31261 ccgttttcca tgccagtggg gcgacgcgcg gcaaggcact atccggcaag ggttgcgctt
    31321 catcagccat aatccggcga atgatccacg ccgcccccga cgacattaaa ggccgttcaa
    31381 gcagcggatc gtcaggctgt aagcgcaatt tgcctgcctt gccgtggcga gcaaacgcgg
    31441 tatacgctgc cgccatatct tccagttttg caccagcgcc gccgagaatg agtgaaagat
    31501 tcggcgcagc gccgttgggc aaatataacg gcaatccaac attgcgtaac tttgccgcaa
    31561 accgtttcgg tccataggct tccagcacct gcacagcagg taagttcagc gagcgcacca
    31621 gcgcctcgct catgctgatc gggccatgaa aaccgctatc aaagttacct ggtcgataat
    31681 caccggtgcg ccgggggacg tcttgcagca gtgatgccgg gtggatcaag ccttcatcca
    31741 gcgccagacc ataaacaaac ggtttgagca ccgatcctgg cgatcggatc gcattgacca
    31801 tatcaacatg accaaagcgt gaatcatcgt tgagatcaac cgatcccacc cagccgcgaa
    31861 cacgcatatc ggtatgatca accacgatca tcgccagtga gctgcgcggt ggcaaccgcc
    31921 ctttccagtt ttgcgccagt tcttccagac gtcgttgaag accggcatcc agcgtagtaa
    31981 cgattttgtc gcttttgctt ttaccgagca tcatgcgcga aaacagcggt gccagttgcg
    32041 gcatttgtcg gggtgccagc cagatgggtt cttcccttga ctcttttacc tgctcacggg
    32101 accacacacc ttgcgcagcc atccgttcga gcactttatt acgcgcggct tcggcacgct
    32161 ccggccaacg atccgggcga agacggctgg gcgcttgcgg caaaaccgcc agcattgccg
    32221 cctcggaata gcttaaattc gcaggcgact ttccgagata agcccaactt gccgcaccga
    32281 tcccctgcaa cgtaccgcca aacggagcgc ggttaagata caaggtcaga atttcacgct
    32341 tagacagatg ccattccagt tgcaacgcgc gccagagctg gcgaattttg ccgccaaatg
    32401 ttttggggtg aggatcaagc agacgagcaa cctgcatagt gagcgtgctg ccaccggaaa
    32461 taacccgtcc cgaagtgaga tcttgccatg ctgcgcgcgc caccgagaat ggattcaccc
    32521 ccggatgctt ccagaaccag cgatcttcat aattgatcag cgcttcaagg taacgtggag
    32581 aaacatcttc gattgttacc ggataacgcc agatgccgtc agcatcggcg aagcgccaga
    32641 gcggcgtacc atcctgcgcc acgaccactc gtgcgggatt gacttcatgc agcggtagag
    32701 gccagagttt atctgctccc cacgctgcaa gaaaaaggag aaatggcgca gctgccagcg
    32761 ttatccagca gccgcgtttg gttaacaagc gaggcattta cggtcggaca atcagcagat
    32821 cttcagccgc gccggtcgcc cgccattggg gaacatacat tgattccacc atcggttgcg
    32881 gtacctgata cgttcccggc gtcaccgccc gcgccagata caccagcgtt accggttggt
    32941 attcatcaac ggcaaccgcc gccacaaagc gatcgtcacg gaactcaatg tgcttaatac
    33001 tcgcctgctg catctggttc agtaagttct gcacttcgcc accactttgc tccaggctgg
    33061 cgctaccgtt cgccagattc tggttttcca gttccagacc cacaggcagc agatccacga
    33121 ctaacgcatc cggcacgctg ttactggctt ttacctgcaa ccacaccagc accagatcgc
    33181 cgctacgtaa cgagtccagc gatttgctct taccatcagt accaagaata tgacgctcga
    33241 tttgcagcac attgttcgcc ggtaaaggtg cggattgcgg ataaccgctg gcatccatac
    33301 gcaaccataa cggctgatcg ccactgttgc tcacctgcaa ggtgacaagt tgatcgctat
    33361 tcagattgct gttttgcgct ttctcgcctg tcagcggctc agctgagaaa gaggtttgcg
    33421 cctgccattt acccggtaaa tcctgaatcg tacgggcagc caggaacaac gcgttacttt
    33481 cctgcgtcga tagccagcgt tcaccaaacg cctgctgcga aagtgtgttc agcaaagtgt
    33541 actgctcatc gggtagcagt ttattttctt ccagcaagga gagcattaac gcgttgtcgc
    33601 gcagtggact accgtaatca cccagccata tccgctcatc actatttctc ggcgttttca
    33661 gcgccagcgc aatcgcttct tcaccacgcg tcgcatcacc catggttttc agcgcaatgc
    33721 caagttgcag cagcggtaaa ccagaagcgg catctgcacg atgctcccag atttcacgca
    33781 gcgcacccag tggtgctttt tgctgacggg ccagcaccag cgcagcgtaa gactgaacgg
    33841 cgaatttact ggctttgaga ttatccgcgt acgggatcga catcatgccc ggatcttgta
    33901 aatagcgcag cagacgctca ttaccccggt taatggcgtc tgtcggcacg ctgtaaccct
    33961 gttcgcctgc gcggaccagg aaatccatca cgtaagccgt cagccagtac tcttcgtcac
    34021 cgtttttatc ccacagcgca aagccgccgt tatcacgttg catttgcagc aaacgggaaa
    34081 tgccgatatc gaccgatgca cggcgtttct catcactgtc gcctttgatg cccaacgcct
    34141 gcagttgggc tgcgttggta taaagtgacg ggaacaggcc gctggcggtt tgctcaagac
    34201 agccgtacgg atacgctttt aactctttga tataacgtgc aatgttcagc ggtggtttgc
    34261 cgctcaacaa caattgccct tccagcgtaa caggcgagaa gttttgcaat ccatccgccg
    34321 gaatcgccca tgtctcacca ggctgtaacg ccgtaccgta attaaccgtt tgtgccggga
    34381 acgccggacg gacgccgatt ttccactgct tatgctgatc ggcaacggtt tcacccggta
    34441 acgctaaccc gctaatggtg gcctgaattt ctccatcgcc ataacccggc aatgctcgca
    34501 ccgggataaa cagcgtagta cgcacacctg gcgctaattc aacggctgcg ggtgaatcgc
    34561 tgaccagttc aagcaaacca ctggcggtca gggcaacgtt cagtttttgc ggtttatcgg
    34621 taagattagt gatatccagc gtcagacgcg aggtatcgcc actcgccata aagcgcggca
    34681 tgttcagttc agcaatcacc ggtgcggcaa ctatcacttt gctttcgttg ctaccgaaat
    34741 catctgccgt ccaggcttgc gccatgacgc gcaattcacc gttaaaatcg ccaatcggca
    34801 gtgtaaccga gccttcgccc tgttcgttga gcgttaccga cagcgcctgc tgcgcgacaa
    34861 tattgacgtg attgaccggc ggtttaccac cacgtttcag ctcatcacca tcgccaccga
    34921 aacgcagagc tgccagacgc ccctgacctt caataacctg accgtaaata tcgtaaatgt
    34981 ctgcgccata gcgtttctga ccaaagaacg cctgccacgg atctggcgta acgtagtcag
    35041 taatattcag cacaccacta tcaacggcag acaccagcac attcacctgt ttaggcttct
    35101 cgccattttt agtgctggct ttaattttca cggttaatgg ttgattggga cgcatttttg
    35161 ctggtgtttc cagcgccaga tcgaggcgac ggttttcatc accaagcggc agatgcaaca
    35221 caccaaccgc gcgttttggc gtcgcggagc gagatttatc gccaggacgc accaccagcg
    35281 tactcaaata aagatcatga cgattccagg ttttatcgac cggaatcgtc agatccagcc
    35341 cttgagccgg aacatcaatc tcttgccacc acagcggccc ttcactggac tcgaccatcg
    35401 cataaccttt acccgccgtt ggcgcggcaa tatgcaactt aatggtatcg ccggggcgat
    35461 aactggcttt atctagtttc agcgtgacac ggtcgggtcg cactgcgccg ctcccgtcgc
    35521 tgttgtcctg ccagctatag ccagcccaaa aacgcacgct actgaccgct tcattcggcg
    35581 ctttgacttc cagacgataa gcgccccact ctaccgggaa actgacctta ccggtttcat
    35641 ccgcctgaag atccagagtt tgttcatttt cgatcagatc tttttgatca aactgtgact
    35701 gccagccttc atcttctgac cagttccagt agtaatcgcg gcgttcgcga atcaggcgca
    35761 cctgcaagcc cgacacggct tttttcacgc cttgcgcatc gctataaacg atgtcaaaag
    35821 cggcgttact gccttcatca acaatgggct gtttcaccgt gctgtcagtg cgataatcgt
    35881 aaaccgattt caaggcgaac tgcggacgga gccccggcaa tgcatcggca ggccagatag
    35941 cctgctcagc gcggcgcgtc accgggcgac cgcccgattc cagcaaactg ccctggaaaa
    36001 taacctgcaa tggggaatgc gtttccttcc actggctttc tgtagaaact tcgccgcgcc
    36061 ctttatcatc cagcgtcaac tgaacttcat ccagcgtgcg ggaaaggttt tcggcagcta
    36121 tatcgccgaa ttcaaaacca ggtaaggctg acacagcctc acgcagtggg cgcaggaaaa
    36181 gttgcccttg caaagtatta ccattagcag gcgcaccgta caggtagtat cccaccacgg
    36241 agaatttcac ttcatcattc ggcgtaagcg gggttttctc accggtcaga ttcagcgcca
    36301 tacgctctgg cataaaatct tcgacgtgga aatcccacat ccggtactgg ttatcgcccg
    36361 tgttggcgcg aatatgccac ataccggttg ccgcattgct atcgagtggc caggtaaagt
    36421 ggtagaggcc attctccggc tgactaacga cgttcctgag tacctgcccg tcaggtttaa
    36481 tcacgtctaa cttgatgggt tgatcgggca atgctttacc gtctgcatcg cgcagtaaac
    36541 cattgaggat taccgtttca cctgggcgat aaagatcgcg tgggccaaac atgaaaaact
    36601 gtttgctata gcctggcgcg ccagcaatgt taaattctgc taagtccagc gccggaagtt
    36661 ttaaatcgag tagcgttgtc tgaccgtctt tacgtgccag cagtaatgcc gcatttttat
    36721 cattttccag ctgcacatgc ccctgagcgt cactggttgc ctgagtcaga gtctgtcctt
    36781 tctcatttaa taaagagact tcaattcctt gctgggccgc gccgttttcc agactttggg
    36841 taaagatatc cagacgattg tgataacggt gagctgaaac gccgatatca cttaacgtaa
    36901 acagcgtcgc gggattactg taatcgtaac gtccagcctg attcatcaca gccagataca
    36961 cgcccgcctg ctgaagcggt ttgatatcgc ccagcggcag caataatttt tcacgggtgt
    37021 tacgcgcagg attgagatca aaccgtccgg tgtagaccag atccgccatc tgcagcagtt
    37081 tgtctgactg ccagttcgcc agcgaattgc ggtattccca ttggctaatg aatgctggca
    37141 gagattctgg cttaacgcgg aagaagttaa catcgacatt attaacgttg agcgccatta
    37201 ccggcagccc ttcaacgact ttgccaggca gcagcgaacc acggctggca aaaccgacgc
    37261 tgggttggat gtcgcgggta gttatagttt tttcgtaatc tttactgaag gttgcgttgt
    37321 tgagtgcttt gacctccttg ccaatagtaa cgatcaaatc acgtttcggt tcgaggtggc
    37381 gtaaacgtag ctctttaaga ttatctgaca gctcccaggc accatccact ttgccgcttt
    37441 ttttatcgac gacatgaata acgcgtgaga aatcctgatc cgggtcgaga gggatggaga
    37501 acgtcagcac cagcgtggct gcaccatcaa gttggacttc agagagatcc agcaatgtca
    37561 gcaccttacc ggcactctgt tgggccagtt tttgtctctc cggcacggag agttttgctg
    37621 aactcgcgtt ttctgaagag gccgctttag taacttcaga aggcgcatct tttttcaccg
    37681 ctgttggcgc gttatcgttg ttgtcgcacc ctgccagcgc cagcattagc atgcaggcgg
    37741 ctacgcgtaa ctttttcatt tttcatccct gccacaatgg cccgttagca acgtcgaata
    37801 attattatgc gtgagaattt acgttatgta attcagtatc acgcccatac aatccgaatt
    37861 ttagtgagaa ttgtggctca gacagccaaa tcagcctctt tcgcttgtcg cttttcacta
    37921 aacatccgac aatttagcct aaccccggca aaaatggaga tgcctatgtc cacgacatgg
    37981 tttgtaggag ccgactggct cgccgaacat attgatgacc cggaaattca gattatcgat
    38041 gcccgcatgg cgtcgcctgg acaggaagat cgtaacgttg ctcaggagta tctgaatgga
    38101 catattcccg gcgcagtgtt ttttgatatc gaagcgcttt ctgatcacac ttccccgctt
    38161 ccgcacatgc tgccgcgccc ggaaacgttc gccgtggcga tgcgtgaatt aggcgttaac
    38221 caggataagc acctgattgt ctatgacgaa ggtaatcttt tctcagcccc acgagcatgg
    38281 tggatgctgc gcacctttgg tgtagagaaa gtgtcgattc tggggggtgg acttgcaggc
    38341 tggcagcgcg atgatctgct gttagaagaa ggtgcagtag agctgccgga aggagagttt
    38401 aacgccgcgt ttaatcctga agccgtggtg aaagtaaccg atgtattatt ggcaagccat
    38461 gaaaatacgg cgcaaattat tgatgcccgc ccggctgcac gttttaacgc agaagttgat
    38521 gaacctcgcc caggtttacg tcgcggacat attcccggag cactgaatgt tccgtggacg
    38581 gaactggtgc gcgaaggcga actaaaaacg accgatgaac tggatgcgat attttttggt
    38641 cgcggcgtca gctacgacaa accaattatc gtcagctgcg gctctggtgt aacggcagcc
    38701 gtggttttgt tagcactcgc gacgctggat gtgccaaacg tgaaactgta cgacggcgca
    38761 tggagtgaat ggggcgcgcg ggcagattta ccggttgagc cagtgaaata agtattttac
    38821 aggcaataaa aaaccgccga atttggcggt tttttattgc tggtctggtt cgcggccttt
    38881 ccagcaggtt gtattaccgt agtaatgcaa gcgcgtctca gcggagacaa tactcgccag
    38941 taactctctt tttgtcaagc aaaagagagt tattattgtt ctgttagtgt attatccact
    39001 gcggcccttt ccgccgtctc gcaagcgggc gctggcttaa ggaaaggatg ttccgcgacc
    39061 gttagagcaa gcgcgtctca gcgcttgcta tcgcggcaat atcgccagtg gtgctgtcgt
    39121 gatgcggtct tcgcatggac cgcacaatga agatacggtg cttctgtatc gtacttattg
    39181 tttctggtgc gctgttttcc caagtaaata ataaccggag tctctccggc gacaatttac
    39241 tggtggttaa caaccttcag agcagcaagt aagcccgaat gccgcccttt agggcggcat
    39301 attttagatt atccggtttt gtttaaagtc acgcaaaaaa ccaccccagc gacgttcata
    39361 gaatggcaca atatgttcgg taataaagtg gctaattcct ttttcccctt ttttcacctg
    39421 acaaatatcg attggttcat cgccaggtaa tgtatcggtc gctacacttc ccgtcgcctg
    39481 aataatttct tcgatatcac catcggcttc aatgccaata agtaaattag gctgtgcctc
    39541 ttcgttctct ttaattgaac aaataaaagc acgcttcacc ggcttaatgg ttttaaataa
    39601 ggtggtgagt gaatcaatca tttgtgctgg cggctctgcg acttccgata atatcagcga
    39661 ttcaccgcct tccaggattt cctggctgct cagcggattt ccctcttcac caatcaacaa
    39721 actgatttca cgcggcataa attctttacc ggtcggcagt ttggcattaa ggaagagcgt
    39781 ttcgccaagg gtcatctcaa acaatgtgcg aacgggcatt acgacaaatg cctgttcgtc
    39841 ttcaaccgcc tgttgaagcg cttctaacga ggtgaaaaaa ggaatgacgc tggtgccgtc
    39901 ttctttttcc cagtgctgta aatcaagcgc actatcttca accacagcct cgccctgcgc
    39961 cgccgtacca ggcacccaga cggtggattc cagtagagta cggaaaaagg ccgggcggtg
    40021 cgccggttcg gttgctgctt tttccagcag gtcttcaagt tcgttttttg tttcggacat
    40081 aagaaccaca attcattcaa cgttcggggc gcaaatgcct gatgcgctac gcttatcagg
    40141 cctacaaggg attcgcaatt tgttgaattt gcagaatttg taggccggat aaggcgttta
    40201 cgccgcatcc ggcatattag tttacgccgt taacagatta gctatcgtgc gcacaccaag
    40261 tcccgtagcg cccgcagacc actgttcaac cggcgcttta cggtaagtcg ccgagcagtc
    40321 gatatgcagc cagccttgct gatagttctc aacaaagtgc gacaggaagc ccgccgccgt
    40381 gctcgcgcct gccggatacg ccgcgcttcc ggtattgttc agttcggcaa agttagacgg
    40441 cagctggctg cggtggaatt ccgccagcgg cagacgccag aatggttcgt tctcttgtga
    40501 ggcactcgcc agcaaacgac cggcaagcgc atcgtcaaaa ctgaacagcg cgtgataatc
    40561 attacccagc gcagttttcg ccgccccggt gagggtcgcc gcatcaatga tcatttccgg
    40621 tttctgcgca ctggcatcaa tcagaccatc ggcaagcacc agacgccctt cggcatcagt
    40681 gttcatcact tcaacttttt taccgttgcg ataggtgatg atatcgccca gcttgaacgc
    40741 attgccgcta atcaggttat ccgcacagca gaggaacagc ttcacgcgct tgttcagtcc
    40801 gcgcgtaatg gcaaatgcca gcgccccggt aaccgttgcc gcgccgccca tgtccgactt
    40861 catcgagtcc ataaacgcag tctgtttgat gctgtagccg ccggagtcaa aagtgatacc
    40921 tttacctacc aggcacgcgt acactggcgc ttctttatcg ccagttgggt tgtaatccag
    40981 cgccagcaat accggagaac gttctgaacc gcgtccgacg gtgtgcagcc ccatataacc
    41041 ttgctcacgc agatcttcgc ctttggtgat ccgataagtc acacgatcgc ccgcgacgtt
    41101 gctgatcaga tcaacagcac gctgtgccag ttgcgatggt cccaattctt ctgccggtgc
    41161 gttgatggtg tcacgcaccc agtcgatgat catcaggcgg ttatccagtt cctggcgctg
    41221 ggcatcgtcc agatccggcc actccacttt acgcgtgcct ttcggggctt tgtaaccttg
    41281 ccagaatgcc cagcagcgat ccgcatccca gccttcaccg cttaactgaa catgcttgat
    41341 gcccagaccg tcaatcttgc gcgccgcacg ctggatcagc cccagatcgt ctgccccgtt
    41401 caaatgcagg gtaatgccgt cattattaat gctgtaagtt gctttttctc cccagcgcgc
    41461 gtcggcaggt tgggtagaga gggtaatctt catcgcttct gtcattttag ttatccttct
    41521 ttgtaaaagg gccgccagtt ggcagcccca ggatttttcc gctaaatgat tcgcgttgca
    41581 gaaaggcggc aagtgagtga agccccagga gcatagataa ctatgtgact ggggtgaacg
    41641 agcgcagcca acgcatctgc ggcgtgaagc atgacgcgga aattattcgg cctcgtccag
    41701 ccagactaac aaaatcgctt cgaggatttt ctcgttggat gcctgcgggt cgtcgtcgaa
    41761 atcttccaga tcgcaaatcc actgatgcat atcggtgaat cgaaccgttt tcggatcaag
    41821 atcaggatac gcatcgtaca gtgcttcgcc aatttcgcgg ctatcggtcc acttaagtcc
    41881 catactaacc tctgttaatg ctcacgcgca tggttgatag tgtaacgcgg gatttcgact
    41941 actaaatctt cgtcggtaac acgtgcctgg cagcttaaac ggctttcggg ctccagtccc
    42001 caggctttgt ccagcatgtc gtcttcctgc tctgagcttt ctggcagcga gtcaaaacct
    42061 tcacgaacga tgcagtggca ggtggtgcaa gcacaggatt tttcacaggc gtgttcaatc
    42121 tcgataccgt tacgcagagc tgcgtcgaga atggtttcac cgctattagc ttccagaaca
    42181 gcgccatcag gacagagatc ctgatgaggc aaaataacaa tctttggcat attaaacctc
    42241 gtccacggaa tggcctttca gcgcacgacg aaccgactgg tccatgcggc gagcggcgaa
    42301 atcctgggtt tgtttgtcta cgtttttaat cgcttgttcg atggcgtcaa cgtcatcgcc
    42361 ctgcgccact tcactcaggt gagcggcagc atcgtcaatg acctgacgtt ctgcggcgct
    42421 taacagcgcg gcatcagcag ccagcgcgcc gtgcagactt tccagcacac gcgccgcttc
    42481 tactttttgt tctgccagca ttcgggcttt tacgtcctgc tcggcatagc tcattgagtc
    42541 tttgatcatc gaagcgattt cgctatcggt cagaccgtaa gacggtttga cctgaataga
    42601 cgcctcaacg ccggtggatt tctccatcgc cgtcacgctc aaaagaccgt cggcatcgac
    42661 ctggaacgtc acgcgaatat gcgcaccacc agccggtagc gccggaatac cacgcagcgc
    42721 aaaacgcgcc agtgagcggc agtcctgcac cagttcgcgc tcaccctgca ttacatggat
    42781 agacatcgcc gtctgaccat ctttaaaggt ggtgaaatcc tgagcgcggg ccaccggaat
    42841 agtggtatta cgcggaatca ctttctccac caggccgccc atcgtttcga ggcccagcga
    42901 cagtgggatc acatcaagca gcagcatttc gctgtctggc ttgttaccca ccagaatatc
    42961 cgcctgaatc gccgcgccaa tagcgacgac tttatccggg tcgatggaag tcagcggtgg
    43021 acgaccgaaa aattcgccta cccgttcacg caccagcggc acgcgagtag aaccgcccac
    43081 catcaccact tccagcactt cgtcagcttc tacacccgcg tctttaagcg cgcgacgaca
    43141 agccagtaag gttcgtttta ccagtggcgc gatcagttca ttgaattgtt cacggctgat
    43201 ttcgccctgc cagcccgcaa cgttaacggt cacggagtcc gcatcgctca gcgcgatttt
    43261 ggctgcaatg gcggcatcca gcagttcacg ctgaacgcgg ttatcgctac gatcaggaat
    43321 gcccgcctgc tcgcgaatgt aatccgccag cagatggtcg aaatcatcgc cgccgagcgc
    43381 ggaatcaccg ccggttgcca gcacttcaaa cacgccgcga cttaagcgca gaatggaaat
    43441 atcaaacgtc ccgccaccga ggtcataaac agcgatcacg ccttcctgac cggaatccag
    43501 cccgtaggcg atagccgcag cggtcggttc gttaagtaag cgcagaacat gcaaccccgc
    43561 cagacgcgcc gcgtctttgg tgccctgacg ctgggcatcg tcaaagtacg ccggaacggt
    43621 gataactaca ccatccagct cgcctgccag ggcttcagtt gcccgcgccg ccagtgcttt
    43681 gaggatgtcc gcagaaacgc gcaccgggtt cagcagcccc gccgccgttt caatcatcgg
    43741 caggccgttt tcgctggcct ggaattgata aggcagatgc ggatagcgtt gctggatatc
    43801 agccagcgag cgccccatca ggcgtttaac agaactaatg gtgttggcgg tgtcaagcgc
    43861 tgcgttagta cgcgcgtcat aacccaccga atgcccttgc tgttgatagt gaacaacaga
    43921 tggcagcagg tgacggcctt catggtcggc taacgtttcg gcctgaccgc tgcgcactgt
    43981 cgccaccagc gagttggttg tgcccaggtc aataccagcc gccagacgac gctgatgcgg
    44041 cgcagcactc aaaccaggtt cactaatttg taataaggcc atgtttagct tccagaaatt
    44101 aaaaatcgag cagtttttct tcgagttgtt cggcactgct ccgcagttta tcgagaaaac
    44161 gcagcttacg cacggtatcc gccgccgcgt cccacgcctc gttgtctaac tgttcaacca
    44221 tcaactgatg gcgggtatca aacatctttt tcacacgttt gataaagctt tccagccgcg
    44281 cttcatcttt cgcctgttcg atctcgtcca gctcttcgcg cagctccaac tgttccatca
    44341 ggaacgcggt gtcgcgcaca gtatgctgct cgctggcgag atcaaaaccg tgtaaagaaa
    44401 gcaaatattc cgcgcgcatt aacggatgac gcagcgtttg ccaggcctgg ttaatggttg
    44461 cagattgctg tacggcggcg agttgttccg cctggcttcc gctggcgaat ttatcaggat
    44521 gatactgacg ttgtagatcc tgaaaacgca ggctcagcgc ctgggtatcg agttgataac
    44581 gggcaggcaa gccaaagaag gtgaagtaat ccataacatt ctcagggctg cgatccgccg
    44641 cgataacggc gaatcaggta aaacaaaccc cacgcgcagg cgaccacggt ggggttatcg
    44701 gtatgcgcat caaacgtgga agctttcgcc gcaaccacac tcatctttga cgttcgggtt
    44761 ggtgaattta aacccttcgt tcaggccttc ttttacgaag tccagctgcg taccgtccag
    44821 aaattgcagg cttttgccat cgaccacgac tttcacgcct ttgtcttcaa acacgatgtc
    44881 ttccggcgtc ggttcgtcaa caaattccag tacataagcc atacctgaac atccggaggt
    44941 tctcacgccc agacgcaggc caaacccttt gccgcggtta gccagaaagg tatttactcg
    45001 cgctgctgca ctgtcgctca gtgtaatcga cataaccaaa cctcaactct tattttgctt
    45061 cacgtttgct tttatagtcc gcaatggcgg ctttgatcgc gtcttctgcc agaatagaac
    45121 agtgaatttt caccggcggc agttcaagtt cttcagcaat atcggtattt ttgatcgcct
    45181 gcgcttcgtc gagagacttc cctttcaccc attcggtgac cagggagctg gaagcgatag
    45241 cagaaccgca gccgtaagtt ttaaaacgcg cgtcttcaat gataccttca tcgttgactt
    45301 taatctgcaa cttcatcacg tcgccacagg ccggtgcccc caccatgccg ctgccgacgt
    45361 tctcgtcgtt gttgtcaaag gaacccacgt tacgcggatt ctcgtaatgg tcgataactt
    45421 tttcgctgta agccattata aattctcctg attccgatac cgattaatga tgagcccatt
    45481 cgatgctgtt cagatccacg ccctgcttgt acatttccca cagcggagaa aggtcacgca
    45541 gacgaccgat ggatttacga actaactcga tggtgtagtc gatctcttct tcagtagtaa
    45601 aacgacctaa agagaaacgg atagagctat gtgccagctc gtcgttcagc cccagcgcgc
    45661 gcagcacgta ggacggttcg aggcttgctg acgtacaggc ggaacctgaa gaaactgcga
    45721 ggtctttcag cgccataatc agcgactcac cttcaacgta gttgaagctg acgttgagaa
    45781 tgttcggcgc accgtgttcc aggtcaccgt tcaggtaaac ttcttcgata tctttgatgc
    45841 cgttccacag acggttacgc aggccgcgca gacgttccat ctcggtcgcc atctcttctt
    45901 ttgcgatgcg ataggcctcg cccattccga cgatctggtg aacaggcaga gtgccggaac
    45961 gcataccgcg ctcgtgaccg ccgccgtgca tttgcgcttc gatgcgtacg cgcggtttac
    46021 gacgtacata cagcgcaccg atacctttcg ggccatagat tttgtgaccg gagaaagaca
    46081 tcaggtcaac tttcaactgg ctcaggtcga taggcagttt gcccacgctc tgggttgcat
    46141 caacgtgata gataatgcca cgagcacggc acatttcgcc gatagccgcg atatcctgca
    46201 ccacgccgat ttcgttattt acgtgcatga tggaaacaag gatggtgtca tcacgcatcg
    46261 ctgcttcaag ttctttcaga tcgataatgc cgttacgctg cggtgccagg taggtgactt
    46321 caaaaccttc gcgctccagc tgacggcagg tatccagtac cgctttgtgt tcggttttgc
    46381 tggtgatgat gtgcttgcct tttttctgat aaaagttggc tgcacctttg atcgccaggt
    46441 tgtcagattc ggttgcacca gaggtaaaga cgatttcacg cggatcagcg ccgaccagat
    46501 cggcaatctg attacgggcg atatctaccg cttcttcagc ctgccagccg aaacggtgag
    46561 aacgggaggc cgggttacca aaggttccgt ccatcgtcat aaactgcatc attttctcgg
    46621 caacacgcgg gtccaccggc gtggttgcgg agtagtcgag ataaatcggt aatttcattg
    46681 ctctataaac tccgtacatc actcaatgca aggaaccagg ctaccggctg gatgtacgac
    46741 cgtgtttacg gagcgcgata cgccccggcc tgattctaat atcttgttat ctttaaccta
    46801 actgtttcgg gtttcagcaa gttgacaaac ttacgaatct ccgggagctt acataagtaa
    46861 gtgaccggag tgagtatgtg cagtcaacgc agatgcaaca cgaaacagga cggttaaatt
    46921 aagcgcgtaa cttaacgtcg atcgcgtctt gtatgcgggt gcgtggcgcg tcgtgagtat
    46981 gctgacgacc agacacatcc agcacttcct ggttattaac cagttcgcct aaagtaatgt
    47041 tgttgagaaa accggtgaga cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt
    47101 tatcgccgcc ctggcaaccg cctttaccct gacaacgggt agcatctaca gattcgtcaa
    47161 cggcgctaat cacttcgcca acggcgatgc tgctggcatc tttgcctaac agataaccac
    47221 cgcctggtcc acgtacgctg gaaaccagac catttttacg cagacgggaa aacagttgtt
    47281 ccagataaga aagggaaatt ccctgacgtt cggaaatatc agccaacggt accgggcccg
    47341 cttcagagtt gagcgcaacg tcaagcattg cggtcacggc atagcgccct ttagatgtca
    47401 gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg
    47461 ggggtattgc atagcagggt caagtctgac attcccgagt aaattggtca actatttact
    47521 tgactgattt agtcgggtat ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc
    47581 tgttgtcctt ccagaccatg ggcgggttga gggcaattat tcgcattagc gctggcctct
    47641 tcccctcacc ctaaccctct ccccgaaggg gcgaggggac cgtccgtgca tttatctggc
    47701 ttagccctca ggccttatta cctttattct gctgctctat agaagccaga atcccgcgca
    47761 ggatattcaa ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca
    47821 tcacctgccc cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat
    47881 gaccataaaa acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct
    47941 gctcgccatt ttcctgagtt gccagccagg ccatacgcac ttcataggca ataacctgaa
    48001 ccgccattgc caagttcagc gaactgtatt ccgggtttgc cgcaatcgcg acatgataat
    48061 ggcatttctg caactcttca ttggtcaagc cgacgcgctc acgaccaaac accagcgcca
    48121 ccggggtatt tgccgcttca gcgacgcttt tcagaccgca ttcgcgcggg tcgagcatcg
    48181 gccacggcag cgtgcgggag cgtgcgctgg tgcccaccac caggctacaa ccggctaacg
    48241 cttcgtcgag cgtatcgacg atgtgagcat taccaatcac atcgctggcc cctgctgcca
    48301 gggcaatcgc ctgggagtcg ggtttcacca gtggattaac cagccacaga ttggttaatc
    48361 ccattgtttt catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca
    48421 gcacaattcg aatattttgc agcattgtct ttcttcatct aaagattatt cacgcatctt
    48481 atcataaaac gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct
    48541 ttaacatcca gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc
    48601 gcaaggcggg taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc
    48661 agaaaggcag taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg
    48721 acacgattcg taaatcttac tcacagcaca ccatcatcac cgaagaaagc ggtgaacttg
    48781 aaggtactga tcaggatgtt caatgggtta tcgatccact ggatggcact accaacttta
    48841 tcaaacgtct gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag
    48901 ttgctgtggt atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg
    48961 cacagctgaa cggctaccgt ctgcgcggta gcaccgctcg cgatctcgac ggtaccattc
    49021 tggcgaccgg cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg
    49081 gcaaactgtt caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg
    49141 cttacgtcgc tgcgggtcgt gttgacggtt tctttgaaat cggtctgcgt ccgtgggatt
    49201 tcgcggcagg cgagctgctg gttcgtgaag caggcggcat cgtcagcgac ttcaccggtg
    49261 gtcataacta catgctgacc ggtaacatcg ttgctggtaa cccgcgcgtt gtgaaagcca
    49321 tgctggcgaa catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg
    49381 tgatgtcact cacccgccct cgcctttcag gcgctattct gaaatacttc ctcaccgctt
    49441 tactttcttt cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcac
    49501 aaggaaaggt tatggcgctg ccagtgaaca aacgcgttct caaaattctg tttattctct
    49561 ttgttgttgc cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc
    49621 ctgacgataa aatttacggt cccgacccct ggtcggcgga atccgtcgaa tttacggcta
    49681 aggacggtac tcgtctgcaa ggctggttta ttccttcttc gacgggccct gctgacaatg
    49741 ccatcgcaac catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc
    49801 tggtcagttg gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg
    49861 gtaaatcaaa aggcacgccg tcccaggccg gattgctgga cgatacgcaa agtgccatca
    49921 atgtggtgcg ccatcgcagt gatgtaaacc cacaacggct ggtgctgttc gggcagagta
    49981 ttggcggggc gaatattctt gctgttattg gtcaggatga tcgtgaaggc atacgtgcgg
    50041 tgatcctcga ctccacattt gcctcttatg caaccatcgc caatcagatg atccccggca
    50101 gtggctactt acttgatgag agttacagcg gcgaaaatta tatcgccagc gtcagcccga
    50161 tcccgctttt actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa
    50221 agttgtatag cctggcaaaa gagacaaaac ggttgatcct aatcccggat ggcgaacaca
    50281 ttgatgcttt ttccgatcgt cacggcgatg tttatcgcga acagatggtg gactttatcc
    50341 ttagcgcgtt gaatccgcag aactaaccca tgatcgctag cacgataatc attcacaaaa
    50401 ccaccttaag acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga
    50461 cgcttgccaa catttctgat gattagcatt cccttcgcca tttccctgag caaactttag
    50521 ctattcttat caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca
    50581 tctgtccttt cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg
    50641 ttgaccgcca ccatggcaac cttcagcgag cttaatggtg tggatgatga tattgccagt
    50701 cttgatatca gcgaaacagg acgggagatc ctgcgctatc atcaactcac actgacgact
    50761 ggttatgacg gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt
    50821 cactggctac gacgtggttt ccgtctgtgt ccgtcattta ttaccagcca attcaccccc
    50881 gccctgaaga gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat
    50941 ctacaagcgt tagtgaatct ctgctcccga cggctgcaaa aacgctttga aacgcgcgat
    51001 attcatttcc tgtgtctgta tctgcaatat tgtttgctgc aacaccacgc tggaattacg
    51061 cctcagttta atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg
    51121 caggaaattg gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc tgatgagcca
    51181 ctgtttatgg cattactttt ttccatgttg cgggttcccg atccattgcg tgatgcgcat
    51241 cagcgagaca gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg
    51301 ggaaatgttc gtttttatga tgaacagggg ttatgcgatc agctttatac ccacctcgcc
    51361 caggcgttaa atcgcagttt gtttgccatc ggtattgata ataccctgcc ggaagagttc
    51421 gccagactgt acccacgcct ggtgcgcacc acccgcgcgg cgctggccgg atttgaaagt
    51481 gaatacggcg tccatctttc tgatgaggaa agcggtctgg tcgcggtgat tttcggtgcc
    51541 tggctaatgc aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat
    51601 agcgagcgag aagcgcagat tgagcagcag ttacgcgaac taacgttact gccgctcaac
    51661 attaagcata tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg
    51721 attattgcgc cttataccat gccattaccg ctcttttcac caccgctgat ctatacggac
    51781 ctgacgctga caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg
    51841 aacaactttc gggcgcaaaa acattgccgg aagcgccacc agcgccatta cccagaacac
    51901 gccgtggccc agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc
    51961 gcccatcgcg acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgaac
    52021 agcaatatag cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcaaaatttg
    52081 caccactatc aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc
    52141 gcaaatcgcc gagatcaaca gcatatcgcg agcactacaa cggcggaaaa gtttattact
    52201 cagcgcaaag ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga
    52261 ggccgagtag ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc
    52321 cccctgcaat aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca
    52381 cgcagaccaa ccggtgctct cctgctggcg acttgcccct tgtggctgaa tcgtcggacg
    52441 gatgagaaag ccgagcagca tggatgccac gcccaacgtc aacagcgcga ggatcacccg
    52501 ataatcaaac atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga
    52561 gccccacagt cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag
    52621 tgcatcggtc aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat
    52681 cagccacgct acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc
    52741 cagcacgcgc aaggcggaaa tcaggcggga aggatcgctg acgcggggcg cgatgagcaa
    52801 actcccgaga aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt
    52861 taaaccaatc cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa
    52921 gtatgtgaaa tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg
    52981 tcaggaggcg ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt
    53041 cgagcctgct tcccgatgtc tgtaacgcca gttcacaagc actaaaaaac tgcgatccgc
    53101 gacgcatgtt gcaaaaataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac
    53161 aatggcgatg aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa
    53221 agcaatggaa ggcacatttc cggcaatggg acgaaaaaca acctgcccgg tattaaaatt
    53281 attcatataa ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac
    53341 cagaatattc gttgccacct ggacgatatt tggctggctg ttttcttgcg caaaccacgc
    53401 tttaacgatc ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc
    53461 atccagttac gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac
    53521 caccactaat ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg
    53581 gcgcattaag ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat
    53641 taaactcacc agctcaatca aggtgtctgg ctgtctgaga cgaaacattg gtaatacttt
    53701 tggcaataaa ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg
    53761 aacaattttc cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc
    53821 ctggagaaaa cattctcccg ccgccgtcaa cgcgactttg cgcttatccc tcaccagtaa
    53881 cggaacaccg acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt
    53941 atgcagtttt tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg
    54001 taaatgccgt agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa
    54061 attaactatt tcacatatta gcaaccaacc aacaacatcc ttatgacaca aaaatagaag
    54121 gtcaatacat cttatctttc aggattaaaa aatatgacca caccctcaga tttaaacatt
    54181 taccaactga ttgataccca aaatggtcgg gtcactccgc gtatttatac cgacccagat
    54241 atttaccaac tggagcttga acgtattttc ggccgttgct ggttatttct cgcccacgaa
    54301 agccagatcc caaaacctgg tgatttcttt aacacctaca tgggggaaga tgcggttgtc
    54361 gtagtgcgtc agaaagacgg cagcattaag gcgtttctca accaatgccg ccaccgggcc
    54421 atgcgtgtga gttatgcaga ttgcggcaac actcgcgcct ttacctgtcc gtatcacggc
    54481 tggtcttatg gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca
    54541 caagggttgt gtaaatccca ctggggacta aacgaagttc cttgtgtgga gagttataaa
    54601 gggctgattt ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac
    54661 attgcctggt atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc
    54721 ggcgtacaaa agtgggtgat caactgtaac tggaaattcc cggcagagca gttcgccagt
    54781 gaccagtatc atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat
    54841 gatggcagcg ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc
    54901 aaagatgcgc tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa
    54961 ccggatgcta atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatgcc
    55021 gaagcagaac aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatatt
    55081 ttccccacgc tttcatggct caacggcact gccacgctcc gcgtctggca tccgcgcggc
    55141 cctgatcaag ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt
    55201 aaagccgctt ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag
    55261 gatgactcgg agaactggtg tgaaatccag aaattgctta aaggccaccg cgcccgcaac
    55321 agcaaactgt gtctggaaat ggggcttggt caggaaaagc gccgcgacga cggcattcct
    55381 ggcattacta actatatctt ttcagaaact gccgctcgtg gaatgtacca acgctgggcc
    55441 gatcttctga gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag
    55501 gaggtgatga aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc
    55561 tctttcacga agccagctta ctggacgact ggaaatttcg tgactggctg gagcagctcg
    55621 acaaagagat tcgttacacc atgcgcacca cagttaacgc gcaaacacgc gaccgccgca
    55681 aaggcgtcca gccaccgaca acctggattt ttaatgacac caaagaccag ctggagcggc
    55741 gaatcgcccg tctggaaacg ggcatggcct gggcagaaga gccgccgtca cgcacccgtc
    55801 acttaatcag caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgtgcggg
    55861 taaattatct gctttatcgg gcacaaaaag agcgcgatga aacattctat gttggaacgc
    55921 gtttcgacaa agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg
    55981 tcctggatca agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat
    56041 ttatgcgtgt cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc
    56101 cgtcatcgcc ctgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca
    56161 tggtaatgcg tcgatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct
    56221 acacgccgcc agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc
    56281 gctcaccact tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc
    56341 gcagccatga gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta
    56401 gggctggcgc tggtcgagcg atttatcgaa gaaggcgcgc aagttgccac gctggaactg
    56461 tcggcggcaa aagtcgccag tctgcgtcag cgatttggcg aacatattct ggcggtggaa
    56521 ggtaacgtga cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc
    56581 ggcaagctgg attgttttat cggcaatgca ggcatctggg atcacaatgc ctcactggtt
    56641 aatactcccg cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caacgtactc
    56701 ggttacctgc tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga aggcagcatg
    56761 attttcacac tgtcaaatgc cgccaggtat cctggcggcg gtggcccgct gtacaccgcc
    56821 agtaaacatg ccgcaaccgg acttattcgc caactggctt atgaactggc accgaaagtg
    56881 cgggtgaatg gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc
    56941 gggcaaagtg aaacctcgat aatgcagtct ctgacgccgg agaaaattgc cgccatttta
    57001 ccgctgcaat ttttcccgca accggcggat tttacggggc cgtatgtgat gttggcatcg
    57061 cggcgcaata atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt
    57121 cgcggcattc gccacgtagc ggctgggctg gatctttaag gaagcacgat gaaagaaaaa
    57181 acgatcatta ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag
    57241 caagggttca ccggtgagct gcatctgttt tccgatgagc gacatcttcc ttatgaacga
    57301 cctccactct cgaaatccat gttgctggaa gattccccgc agttacagca ggtgttaccc
    57361 gctaactggt ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacgctg
    57421 ggtcgcgaca cacgagagtt agtgttaacc aacggcgaaa gctggcactg ggatcagctt
    57481 tttatagcaa tcggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc
    57541 tgctttaccc tacgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa
    57601 cggtcagtcg tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg
    57661 cagcgcagat gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca
    57721 ccaccgcccg tgcaacgcta tcttttacag cgccaccagc aggctggtgt gcgcattctg
    57781 ctcaataatg ccattgaaca tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt
    57841 ggggaaacgc ttcaggctga tgtggtgatt tacggtattg gtatcagcgc caacgagcaa
    57901 ctggctcgcg aggccaacct tgatactgcc aatggcattg tcattgatga ggcttgccgc
    57961 acctgcgatc ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt
    58021 gcactacacc gctgcgaaag ctgggaaaac gccaataacc aggcgcaaat tgccgctgcc
    58081 gcaatgttag ggctaccgct accgctactg ccgccgccgt ggttctggag cgatcagtac
    58141 agtgataact tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac
    58201 ccggaaactc agaaggcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggtc
    58261 acgctgaatc aggggcgtga gattcgccca attcgcaaat ggatccagag cggcaaaacg
    58321 tttgatgcga aactgctgat agatgagaac atcgcgctta aatcactgta accaggataa
    58381 ttagcgaata tctcaacgcc tggggcgtgg cgaggtgcaa gagtgtgtat tacgtttaaa
    58441 tcacattatc ttgcaaaggg attggttatg aacacacta
//
LOCUS       DAJTVK010000011        57432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000011 DAJTVK010000000
VERSION     DAJTVK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.011"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="OO372_000719"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="OO372_000719"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6340304.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3105)
                     /locus_tag="OO372_000720"
     CDS             complement(1498..3105)
                     /locus_tag="OO372_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6340305.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3583..3669)
                     /locus_tag="OO372_000721"
     tRNA            complement(3583..3669)
                     /locus_tag="OO372_000721"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3633..3635),aa:Leu,seq:gag)
     gene            complement(3684..4016)
                     /gene="secG"
                     /locus_tag="OO372_000722"
     CDS             complement(3684..4016)
                     /gene="secG"
                     /locus_tag="OO372_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6340306.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="OO372_000723"
     CDS             complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="OO372_000723"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6340307.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5574..6422)
                     /gene="folP"
                     /locus_tag="OO372_000724"
     CDS             complement(5574..6422)
                     /gene="folP"
                     /locus_tag="OO372_000724"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6340308.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="OO372_000725"
     CDS             complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="OO372_000725"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6340309.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="OO372_000726"
     CDS             complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="OO372_000726"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6340310.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9301..9594
                     /gene="yhbY"
                     /locus_tag="OO372_000727"
     CDS             9301..9594
                     /gene="yhbY"
                     /locus_tag="OO372_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6340311.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9750..10226)
                     /gene="greA"
                     /locus_tag="OO372_000728"
     CDS             complement(9750..10226)
                     /gene="greA"
                     /locus_tag="OO372_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6340312.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10474..11907
                     /gene="dacB"
                     /locus_tag="OO372_000729"
     CDS             10474..11907
                     /gene="dacB"
                     /locus_tag="OO372_000729"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6340313.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="OO372_000730"
     CDS             complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="OO372_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6340314.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13281..14246)
                     /locus_tag="OO372_000731"
     CDS             complement(13281..14246)
                     /locus_tag="OO372_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6340315.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="OO372_000732"
     CDS             complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="OO372_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6340316.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="OO372_000733"
     CDS             complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="OO372_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6340317.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15221..16192
                     /gene="ispB"
                     /locus_tag="OO372_000734"
     CDS             15221..16192
                     /gene="ispB"
                     /locus_tag="OO372_000734"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6340318.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16421..16699
                     /gene="sfsB"
                     /locus_tag="OO372_000735"
     CDS             16421..16699
                     /gene="sfsB"
                     /locus_tag="OO372_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6340319.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16747..18006)
                     /gene="murA"
                     /locus_tag="OO372_000736"
     CDS             complement(16747..18006)
                     /gene="murA"
                     /locus_tag="OO372_000736"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6340320.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="OO372_000737"
     CDS             complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="OO372_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6340321.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="OO372_000738"
     CDS             complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="OO372_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6340322.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="OO372_000739"
     CDS             complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="OO372_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6340323.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="OO372_000740"
     CDS             complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="OO372_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6340324.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="OO372_000741"
     CDS             complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="OO372_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6340325.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="OO372_000742"
     CDS             complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="OO372_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6340326.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21787..22764
                     /locus_tag="OO372_000743"
     CDS             21787..22764
                     /locus_tag="OO372_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6340327.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22778..23764
                     /gene="kdsD"
                     /locus_tag="OO372_000744"
     CDS             22778..23764
                     /gene="kdsD"
                     /locus_tag="OO372_000744"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6340328.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23785..24351
                     /gene="kdsC"
                     /locus_tag="OO372_000745"
     CDS             23785..24351
                     /gene="kdsC"
                     /locus_tag="OO372_000745"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6340329.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24348..24923
                     /gene="lptC"
                     /locus_tag="OO372_000746"
     CDS             24348..24923
                     /gene="lptC"
                     /locus_tag="OO372_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6340330.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24892..25449
                     /gene="lptA"
                     /locus_tag="OO372_000747"
     CDS             24892..25449
                     /gene="lptA"
                     /locus_tag="OO372_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6340331.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25456..26181
                     /gene="lptB"
                     /locus_tag="OO372_000748"
     CDS             25456..26181
                     /gene="lptB"
                     /locus_tag="OO372_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6340332.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26229..27662
                     /gene="rpoN"
                     /locus_tag="OO372_000749"
     CDS             26229..27662
                     /gene="rpoN"
                     /locus_tag="OO372_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6340333.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27685..27972
                     /gene="hpf"
                     /locus_tag="OO372_000750"
     CDS             27685..27972
                     /gene="hpf"
                     /locus_tag="OO372_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6340334.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28090..28581
                     /gene="ptsN"
                     /locus_tag="OO372_000751"
     CDS             28090..28581
                     /gene="ptsN"
                     /locus_tag="OO372_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6340335.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28627..29481
                     /gene="rapZ"
                     /locus_tag="OO372_000752"
     CDS             28627..29481
                     /gene="rapZ"
                     /locus_tag="OO372_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6340336.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29478..29750
                     /gene="npr"
                     /locus_tag="OO372_000753"
     CDS             29478..29750
                     /gene="npr"
                     /locus_tag="OO372_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6340337.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29964..30596
                     /gene="yrbL"
                     /locus_tag="OO372_000754"
     CDS             29964..30596
                     /gene="yrbL"
                     /locus_tag="OO372_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6340338.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="OO372_000755"
     CDS             complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="OO372_000755"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR6340339.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="OO372_000756"
     CDS             complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="OO372_000756"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6340340.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="OO372_000757"
     CDS             complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="OO372_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6340341.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34633..35562)
                     /locus_tag="OO372_000758"
     CDS             complement(34633..35562)
                     /locus_tag="OO372_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6340342.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36237..40697
                     /gene="gltB"
                     /locus_tag="OO372_000759"
     CDS             36237..40697
                     /gene="gltB"
                     /locus_tag="OO372_000759"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6340343.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40710..42128
                     /gene="gltD"
                     /locus_tag="OO372_000760"
     CDS             40710..42128
                     /gene="gltD"
                     /locus_tag="OO372_000760"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6340344.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42312..42791
                     /locus_tag="OO372_000761"
     CDS             42312..42791
                     /locus_tag="OO372_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6340345.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42808..43113
                     /locus_tag="OO372_000762"
     CDS             42808..43113
                     /locus_tag="OO372_000762"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6340346.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43189..43653)
                     /locus_tag="OO372_000763"
     CDS             complement(43189..43653)
                     /locus_tag="OO372_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6340347.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="OO372_000764"
     CDS             complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="OO372_000764"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6340348.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44522..45211)
                     /locus_tag="OO372_000765"
     CDS             complement(44522..45211)
                     /locus_tag="OO372_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6340349.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="OO372_000766"
     CDS             complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="OO372_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6340350.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="OO372_000767"
     CDS             complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="OO372_000767"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6340351.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="OO372_000768"
     CDS             complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="OO372_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6340352.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49045..50367
                     /gene="dcuC"
                     /locus_tag="OO372_000769"
     CDS             49045..50367
                     /gene="dcuC"
                     /locus_tag="OO372_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6340353.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50410..50907)
                     /gene="sspB"
                     /locus_tag="OO372_000770"
     CDS             complement(50410..50907)
                     /gene="sspB"
                     /locus_tag="OO372_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6340354.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50913..51551)
                     /gene="sspA"
                     /locus_tag="OO372_000771"
     CDS             complement(50913..51551)
                     /gene="sspA"
                     /locus_tag="OO372_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6340355.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51946..52338)
                     /gene="rpsI"
                     /locus_tag="OO372_000772"
     CDS             complement(51946..52338)
                     /gene="rpsI"
                     /locus_tag="OO372_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6340356.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52354..52782)
                     /gene="rplM"
                     /locus_tag="OO372_000773"
     CDS             complement(52354..52782)
                     /gene="rplM"
                     /locus_tag="OO372_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6340357.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53001..54128)
                     /gene="zapE"
                     /locus_tag="OO372_000774"
     CDS             complement(53001..54128)
                     /gene="zapE"
                     /locus_tag="OO372_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6340358.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54322..54720
                     /locus_tag="OO372_000775"
     CDS             54322..54720
                     /locus_tag="OO372_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6340359.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54874..56241
                     /gene="degQ"
                     /locus_tag="OO372_000776"
     CDS             54874..56241
                     /gene="degQ"
                     /locus_tag="OO372_000776"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR6340360.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56331..57398
                     /gene="degS"
                     /locus_tag="OO372_000777"
     CDS             56331..57398
                     /gene="degS"
                     /locus_tag="OO372_000777"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6340361.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14265 a        15302 c        14255 g        13610 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaatcttct agcaaatttg ttgaatactg
     3121 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
     3181 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
     3241 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
     3301 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
     3361 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
     3421 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
     3481 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
     3541 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
     3601 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
     3661 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
     3721 gctggctgag tttgttcggt tttcgccggt gcactcagat tttcccattc gctaccttta
     3781 ttggttttgt tgctattgat gttacccagc accagactga tgataaagaa taacgttgcc
     3841 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
     3901 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
     3961 agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc gtacataatc
     4021 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
     4081 aatctttccc actgaagtgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
     4141 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
     4201 aaaaggcgac aatcgccgcc tttttagcca gttatttacc gttttaaacg gcttttactg
     4261 catcggcgat acggtgtgca aattcagtta cctgcgcttc gtcttcacct tctaccatca
     4321 cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg ctacccagcg
     4381 ccgcttcaac ttcggcggtc acagctttaa ctgactcatg ctcaagcgga tcgccgctac
     4441 ctgcggtgta acgcacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
     4501 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
     4561 cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct gcaccgatac
     4621 gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc gcgcgcgcaa
     4681 atggaattcc taactgtttc aacgccagct caagccccat gttactcatc aatgtaccca
     4741 cagcaccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
     4801 gatcaccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccgtcgccgt
     4861 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
     4921 cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg atagcgataa
     4981 cgttcgcccc cagttcacgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcgc
     5041 aatccaccac aatcttcagt tcgctgaggc taagttcgtt cgggaacgtg gctttgcaaa
     5101 actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt tctgccgaat
     5161 caacacagct gatctctttt tccatttccg cttcgatggc ctcttctacc gcatccggta
     5221 gtttggtgcc gtcgatagag aaaaatttaa tgccattatc gtagaacggg ttatgcgatg
     5281 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
     5341 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
     5401 ctgactccag catatagcca gaaatacgcg tgtctttacc aataatgatc ttacgggagc
     5461 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
     5521 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
     5581 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
     5641 tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat gaccgcacag
     5701 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
     5761 tttcgtgaca tacctaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
     5821 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
     5881 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
     5941 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
     6001 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
     6061 gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc acggatgact
     6121 tctggtttgg atgtatctac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
     6181 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
     6241 gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt cgcatgtttc
     6301 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
     6361 aggatcccca ttacgtgagg atggctaagg tccagtgaag tgccctgggc aaagagtttc
     6421 atggtgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
     6481 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
     6541 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
     6601 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
     6661 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
     6721 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
     6781 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
     6841 gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc gcgtacagca
     6901 gtggacccaa tttctcagag aagccccact gagtcaccat gttacgcgcc aggttggtcg
     6961 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag atgatctctt
     7021 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
     7081 tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
     7141 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
     7201 cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag cgacgttccg
     7261 cacccatcat gattttatct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
     7321 tgccacgagc agcgaacagt gccgcttcgt ttaccaggtt cgccaggtca gcaccggaga
     7381 aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat ggtacgcgac
     7441 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagcca accacaacct
     7501 gacggtcgaa acggccagga cgcagcaggg ccgggtcaag aacgtccgga cggttagtcg
     7561 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
     7621 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
     7681 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
     7741 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
     7801 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc gttttaccgg
     7861 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
     7921 ggctcggctc gcgcagatat tcaaccagtt cagcaacttc ttcttttgct tcgtcgcagc
     7981 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
     8041 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
     8101 acacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
     8161 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
     8221 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
     8281 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
     8341 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
     8401 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
     8461 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
     8521 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
     8581 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
     8641 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
     8701 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
     8761 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
     8821 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
     8881 cgccctgaag aaagtctaca ccaacgatag gatccatagg taaaagatcg caagcgatga
     8941 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
     9001 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
     9061 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
     9121 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
     9181 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
     9241 atatatggga gatggcggta gaatgaccca ttttcaatcc caacgtaagc aaaaaatacg
     9301 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
     9361 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
     9421 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aacaaaaacc
     9481 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
     9541 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
     9601 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
     9661 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
     9721 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
     9781 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
     9841 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
     9901 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
     9961 ccaaaaataa cgcgcccatt gttaggcatt ttggtgacat caatcacctg cgcgtttgac
    10021 agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc acgagctgcg
    10081 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    10141 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    10201 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    10261 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    10321 aaaaaaatac cgacccaggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    10381 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    10441 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    10501 ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta cattactcaa
    10561 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    10621 attgattacc acagtcagca gatggcgctg cctgccagta cccagaaagt gattactgcg
    10681 ctggcggcgt tgattcaact cggccccgat tttcgtttta ccacgacgct tgaaaccaaa
    10741 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    10801 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    10861 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    10921 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc cgccatagtt
    10981 gaccgcaact gtttctccgt ctcgctctac agtgcccaaa aacccggtga tatggccttt
    11041 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccgt
    11101 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    11161 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    11221 gatggagcca gttatgccgg tgcaattctg aaagatgagt taaaacaggc gggtatcacc
    11281 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    11341 aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc ggataacatg
    11401 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtacc tggtacatgg
    11461 cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    11521 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    11581 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    11641 ctgccgctgg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    11701 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    11761 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc aatatctttc tggctatgca
    11821 gtagaacctg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    11881 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggctt
    11941 cagattattg acaaagtgcg ctttgtttat gccggatgcg gcgtaaacgc cttatccggc
    12001 ctacaaaatc gtgcaaattc aacatattgc aattctcttg taggcctgat aagcgtagcg
    12061 catcaggctg atttggcgtt tatcatcagt gattaacgct tgtaaatgaa ctcaacgcct
    12121 tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat catcctcttc agcaatctct
    12181 tcaagctgct ggcgatgata atcgtcccac atgaattcga ctttctctgg ctgtttcgct
    12241 tcttcagcct gcacgaccgg gttttcaagg ataaaggtca tcacatccca gcagagatct
    12301 ttcacgccca gtccgctcgc cgcagagatc agataatatt tatcttccca gcccagcgct
    12361 tcagcaatcg ctttcgcttt ctcttcagct tctgccttat ccagcagatc gatcttattg
    12421 aacactaacc aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata
    12481 ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc
    12541 aacaggacgc ggcaacgttc caggtgcttc aggaagcgga tacccagacc tgcgccttcc
    12601 gcagcgcctt caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt
    12661 cgtaccacac ccagacttgg caccagagtg gtaaacggat aatccgccac tttcggttta
    12721 gccgccgata ccgcgcgaat aaaggtcgat ttacccgcgt ttggcatccc caacataccg
    12781 acgtcagcca gcagcatcag ctccagcagc agctcacgct tatcacccgg cgtaccgttg
    12841 gttttctgcc gtggtgtacg gttaacggac gatttgaaac gggtattgcc cagaccgtgc
    12901 cagccgccct tagcaaccag cagacgctga ccgtgtttag tcatatcgcc catggtttca
    12961 ccagtaccct ggtcgattac acgcgtacct accggcactt taatcgtcac gtctttaccg
    13021 cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc gctctgcacg gaaagatttt
    13081 tcaaaacgat aatcgataag cgtgttcagg ttttcgtcgg cttccatcca tacgtcacca
    13141 ccatcaccac cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag
    13201 ctcacgcaac cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac
    13261 ttcattttat tctccataaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc
    13321 ttacgtaatc cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg
    13381 acaaacgcac cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag
    13441 gccagtgata aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg
    13501 ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca gggcgccata tcctaccaat
    13561 gtattcagtc cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact
    13621 ccaggctttg ccagagggaa gagcgcaatt gtacataaag tgtacagtaa aaacaggatc
    13681 tgcggtgagg ccagccgacg caataaaacc ttttgcgcca cgccataact cacccaaacc
    13741 gtcgccgcac cgaccccaaa gataactccc caggtgtaat cggtgagctt tgtaaatatc
    13801 tcgaccagac tggtgttaaa aaacatcacc aggccgctca ggagcatcaa tgcccctaca
    13861 acctgagtgc tgcgcatttt ctctttcagg ataaatacgc tggcaaccat catgccaact
    13921 ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg
    13981 aacaggatga agttcccaaa cagcccggcg gtcgccactg ccaacaaaat caaccagcgt
    14041 ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat ggcaccaagg
    14101 ccaatactcg ccatcaagaa acggtaaaac acgattgtcg gaggttccat cacctccagc
    14161 acctgcttca ttgcgattgg caacgccccc cagcaaattg ctgtggtgag cgccaaaaga
    14221 atgccaatgc ctgcctgctg cttcataccg tattccctgc aaagcgcatt tgcggattaa
    14281 cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa aagccccgca acgtgttgcg
    14341 gggctttcat ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta
    14401 cggtttttcg ggcctttaac ttcgaatttc actttaccgt ctgctttagc aaacagagtg
    14461 tggtcacgac cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg
    14521 atgatgctac ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct
    14581 tctgaatcgc gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc
    14641 tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatgac
    14701 cctgctgctt acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac
    14761 gaccgtgagc aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt
    14821 tgacttcttc accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg
    14881 cgatgtccag cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac
    14941 cactttggaa aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc
    15001 agagtgcgct ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga
    15061 caagtgctac agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg
    15121 tgccattttt tcagtacaat caccctatat tcctaaccat aaaccctaag ttgcctttgt
    15181 tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa
    15241 tgagttaacc gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc
    15301 cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat
    15361 tcgtccgatg attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt
    15421 caccattgct gccctgatcg agtttatcca cacggcgact ctgctacacg acgacgttgt
    15481 ggatgaatca gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc
    15541 cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc cagatgatga ccagcctcgg
    15601 ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac gtcatcgcag aaggtgaagt
    15661 tctgcaactg atgaacgtta acgatccgga catcactgaa gaaaactaca tgcgcgttat
    15721 ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag tgttccggga ttctggctgg
    15781 ctgtacgccg gaggaggaga aaggcctgca ggattatggg cgctatctcg gcactgcttt
    15841 ccagttgatc gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaatgt
    15901 cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg
    15961 cacaccagaa caggcacaga tgatccgtac cgccatcgaa cagggtaacg gtcgccatct
    16021 tctggaaccg gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg
    16081 tgccgaggaa gaagcagaca aagccatcgc agcattacag gtgctcccgg acaccccttg
    16141 gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc
    16201 cttcatcccg cgcagtgcgc gggatgattc cagtaaattc cataaaaaca ctaattcagc
    16261 gctataacac ctgaaaaaaa agggcgatga atctttatat attctgaata ttcacactct
    16321 ttacaggaac tttttagagc aataggccat caggagtata gtgatgcccg acagaagaag
    16381 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    16441 ctggcatccc gctgacatca ttgcgggttt gcgcaagaag ggaacgtcaa tggcggcgga
    16501 gtctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc
    16561 gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc
    16621 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    16681 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc
    16741 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    16801 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    16861 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    16921 gccagaaagt ttttcaacac cgtgacaaat aacggtattg ctttcgattt cggcatgcgc
    16981 gcccatacgg ctaagctccg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    17041 gaatccggtc ccttctgcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    17101 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    17161 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcgtcacgca gtttcgccag
    17221 cacggcgtcg agagtatctg gctgcgcgtt acggcagata attttgccgc gagaaatcgc
    17281 cgccgccacc aggaaagtac cggtttcgat acgatccggt agcacgcgat agacaccgcc
    17341 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    17401 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcattttc
    17461 aataatcgtg gtaccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    17521 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    17581 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    17641 aaccggacgc gcaccgatcg tacaaccgcc aggcagtgaa acttgcccct gaccaaagcg
    17701 cgctaccagc ggccccagtg cccagataga agcacgcatg gttttaacca gatcgtaagg
    17761 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    17821 cgcacccagc tggcttagca gcttcattga tgtatcgacg tctttcagtt ttgggacgtt
    17881 ctggatctct accggttctt ccgccagtag agcggcaaaa aggataggca gagcagcatt
    17941 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    18001 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    18061 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    18121 cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg
    18181 ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg gcaataacct gaaagtggct
    18241 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    18301 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    18361 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    18421 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    18481 aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac
    18541 cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    18601 tgccagtcca cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt
    18661 caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    18721 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    18781 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    18841 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    18901 gcaatcatgt cgtaagcctg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc
    18961 agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    19021 ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    19081 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga
    19141 gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    19201 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    19261 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    19321 taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg
    19381 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    19441 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    19501 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    19561 ttagtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    19621 aaaccgacgt ttaatgccag atattgttcc cccagcaggc cggaagtacg aatgctcagc
    19681 gaactggtat ctggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga
    19741 taggttttcg ggtccagcgt aatatccgcc acccgaccca caacaacgcc accaatactg
    19801 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    19861 ggttcagtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    19921 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    19981 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    20041 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg tgtcgtagcc
    20101 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    20161 gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    20221 aatgcctttc caactgacgc cgaccagaga tccgccccag atccccacgg cgacgaaaat
    20281 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    20341 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    20401 gatttctgcg gttagcgccg aaccagcacg cccggcaaac aacaacgcgg caaccaccgg
    20461 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcactata
    20521 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    20581 cacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    20641 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    20701 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    20761 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    20821 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgcct
    20881 gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    20941 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    21001 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa
    21061 caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    21121 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    21181 ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcgggt agttgggtat
    21241 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    21301 actgaaataa catgctcatc cgtttgcgca ctgtatacag gcgcgaacga gacatcgccg
    21361 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    21421 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    21481 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    21541 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    21601 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    21661 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    21721 gaatccgtta cggctttctg aaaatcttca gcggaccgac gagtatacct gaagaaagga
    21781 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    21841 gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    21901 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    21961 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    22021 atcaatattt tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc
    22081 gatgttctgc gtcgtgaatt acccttaatg ttgttggtca gcgtagtggc cggttccgta
    22141 ctctatgacg gacaacttag tcgcagcgat ggtatctttc tcctctttct ggctgtgcta
    22201 tggctgctgt tcattgttaa acttgcacgt caggctgaac gtcaggggac tgacagcctg
    22261 accagagagc agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc gtttttatgg
    22321 ctcggcattg cgcttatcat catgccagtg gccacgcgga tggtggttga taacgccacg
    22381 gtgctggcga attactttgc catcagcgag ttgacgatgg gtctgacggc aattgctatc
    22441 ggaaccagcc tgccggaact ggcaaccgca atagcggggg ttcgcaaagg tgaaaacgac
    22501 attgctgtcg gaaatatcat tggcgcaaac atttttaata ttgtcatcgt gttgggttta
    22561 cccgcgctga taacgccagg agagattgag ccattggcgt acagtcgtga ctacagcgtg
    22621 atgttgctgg tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc gcaaccgggc
    22681 cgtggtgtag gggtattatt aactggcgga tttatcgtat ggctggcgat gttgtactgg
    22741 ttatcgccaa tactcgttga ataactggaa acgcattatg tcgcacgtag agttacaacc
    22801 gggttttgac tttcagcaag caggtaaaga agtcctggcg attgaacgtg aatgcctggc
    22861 ggagcttgat caatacatca atcagaattt cacgcttgcc tgtgaaaaga tgttctggtg
    22921 taaagggaaa gttgtcgtca tggggatggg gaaatcgggg cacatcgggc gcaaaatggc
    22981 cgcaacgttt gccagcaccg gtacaccttc atttttcgtc catcctggtg aagccgcgca
    23041 tggtgattta ggcatggtca ccccacagga tgtggtgatt gctatctcta actcaggtga
    23101 atccagcgaa atcacggcct taattccagt gcttaagcgt cttcacgtac cgttaatctg
    23161 catcaccggt cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc tgtgtgttaa
    23221 agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc agcagcacca ccgccacgct
    23281 ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta ctgctgaaga
    23341 ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa cttctgctgc gcgtaaacga
    23401 tattatgcat acgggcgatg agatcccgca tgttaagaaa acggccagtc tgcgtgacgc
    23461 gttgctggaa gttacccgca aaaatcttgg tatgactgtc atttgcgatg acaatatgat
    23521 gattgaaggc atctttaccg acggtgattt acgccgtgtc ttcgatatgg gcgtggatgt
    23581 tcgtcagtta agtattgccg atgtgatgac gccgggggga atacgtgtgc gccctggcat
    23641 tctggccgtt gaggcactga acttaatgca gtcccgccat atcacctccg tgatggttgc
    23701 cgatggcgac catttactcg gagtgttaca tatgcatgat ttactgcgtg caggcgtagt
    23761 gtaaagattc aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc gacctgttac
    23821 ggccctgtca gcgccgacgt tatagcaaaa gcagagaaca ttcgtctgct gatcctcgat
    23881 gtcgatggcg tactgtcaga tggcctgatt tatatgggca ataatggcga agagctgaaa
    23941 gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga tattgaagtc
    24001 gctatcatta ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac attggggatc
    24061 actcacttgt atcaggggca gtcaaacaaa ctgatcgcct ttagcgatct gctggaaaaa
    24121 ctggcgattg ccccggaaaa tgtggcttat gtcggcgatg atctcatcga ctggccggta
    24181 atggaaaaag tgggtttaag cgtcgccgtg gccgatgcgc atccactgtt gatcccgcgc
    24241 gccgattacg tgacgcgcat tgctggcggt cgtggcgcag tgcgcgaagt ttgcgactta
    24301 ttactcctgg cgcagggcaa actggatgaa gccaaagggc aatcgatatg agtaaagcca
    24361 gacgttgggt tatcattgtg ctatcactgg cggttctggt gatgatcggc attgatatgg
    24421 ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa tgatcccacc tataaaagcg
    24481 agcatacgga cacgctcgtc tataacccag aaggggcact aagctatcga ttgattgctc
    24541 aacacgttga atattattcc gatcaggccg tttcgtggtt tacgcagccg gtacttacca
    24601 cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc agataaagcc aagctgacca
    24661 atgaccggat gctctattta tatggacacg ttgaagtcaa cgcacttgtg ccaaactctc
    24721 aacttcgcag aatcacgacg gataacgcgc agatcaatct ggtgacgcag gatgttacct
    24781 ctgaagacct cgtcacgtta tacggaacaa catttaactc cagcggtctg aaaatgcgcg
    24841 gcaacttacg cagcaagaac gccgagctga ttgaaaaggt tagaacatcc tatgaaattc
    24901 aaaacaaaca aactcagcct taatcttgtg cttgccagct cacttctggc cgccagcatt
    24961 ccggcatttg ccgtaaccgg agacactgat cagccgatcc acattgaatc ggaccagcaa
    25021 tctcttgata tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt cacccagggc
    25081 accatcaaaa ttaatgccga caaagtggtc gttacccgtc cgggcggcga acaaggtaaa
    25141 gaagtgattg acggctacgg taaaccggca acgttctacc agatgcagga caacggtaaa
    25201 cccgttgaag gtcacgcttc ccagatgcac tacgaactgg caaaagattt tgtcgttctg
    25261 acgggtaatg cttatctgca gcaggtcgat agcaacatta agggcgataa gatcacttac
    25321 ctggtaaaag agcagaaaat gcaggctttc agcgacaaag gcaagcgcgt aaccaccgtt
    25381 ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc agaccccggc acagaagaag
    25441 ggtaattaat tcgttatggc aacattaact gcaaagaacc ttgcaaaagc ctataaaggc
    25501 cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg gggaaattgt cggtctgctg
    25561 gggccaaacg gtgccggtaa gaccaccact ttctacatgg ttgtaggcat tgtgccgcgc
    25621 gatgcgggca acatcattat tgatgatgac gatatcagtc tgctgcctct gcatgcacgc
    25681 gcgcgccgcg gtatcggcta tctgccacag gaagcctcca ttttccgtcg cctcagcgtt
    25741 tacgataacc tgatggcggt actgcaaatt cgtgacgact tgtctgctga acaacgtgaa
    25801 gaccgcgcga acgagctgat ggaagagttt cacattgagc acctgcgtga cagcatgggg
    25861 cagtcactct ccgggggtga acgtcgccgt gtagaaattg cccgcgcact ggctgcgaat
    25921 ccgaaattta ttctgctcga cgaaccgttt gccggggttg acccgatctc ggttatcgac
    25981 attaaacgca tcattgagca cctgcgcgac agcggcctgg gcgtgctgat cactgaccac
    26041 aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca ggggcatttg
    26101 atcgcccacg gcacgcctac agaaatctta caagacgaac acgttaagcg tgtatacctt
    26161 ggggaagact tcagactctg atagggtaga agtttgcgac gttttagcag gagagtacga
    26221 ttctgaacat gaagcaaggt ttgcaactca ggcttagcca acaactggcg atgacgccac
    26281 agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag caggagctac
    26341 agcaggcgct ggagagtaat ccgctgcttg agcaaatcga cactcatgaa gaaatcgaca
    26401 cccgcgaaac gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa caaaaagaga
    26461 tgccggaaga gctgccgctc gatgccagtt gggacaccat ttacaccgct ggtacaccat
    26521 ccggcaccag cggtgactac attgacgatg agctgccggt ctatcagggc gaaacgacgc
    26581 agaccttgca ggattacctg atgtggcagg tcgagctgac accgttttcc gacactgacc
    26641 gcgctattgc tacctctatc gtcgatgccg ttgatgacat cggttatctg actgtcccgc
    26701 tggaagatat tctcgaaagt atgggcgatg aagagatcga catcgacgag gttgaagccg
    26761 tccttaagcg gatccaacgg tttgatccgg tcggtgtagc ggcaaaagat ctgcgtgact
    26821 gcctgctaat ccaactctcc caattcgata aaaccacgcc gtggctggaa gaggccagac
    26881 tgatcattag cgatcatctc gatctgttag ccaatcacga cttccgcact ttaatgcgcg
    26941 tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa tctgatccag tcgctcgatc
    27001 cgcgccccgg gcagtcgatc cagactggcg aacctgagta tgtcattcca gatgtgctgg
    27061 tgcgtaagca taacggtcac tggacggtag aactcaacag tgacagcatt ccgcgtctgc
    27121 aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg caacgatggt gacagccagt
    27181 ttatccgcag caatctgcag gatgccaaat ggttgatcaa gagtctggaa agccgtaacg
    27241 atacgctact gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc tttgagcaag
    27301 gtgaagaata tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc gaaatgcatg
    27361 aatcgacgat atctcgcgtg accacgcaaa aatacctgca tagtccacga ggcatttttg
    27421 aactgaagta tttcttttcc agtcacatca ataccgaggg cggcggcgaa gcttcctcca
    27481 cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga aaacccagcg aaaccgttga
    27541 gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat catggtggca cgccgcactg
    27601 ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa cagctcgttt
    27661 gacccaaccg ataaggaaga cactatgcag ctcaacatta ccggaaataa cgtcgagatc
    27721 accgaggcac tgcgcgaatt tgttacagcc aaatttgcca aacttgagca atattttgac
    27781 cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag tcacccacac ctcagatgca
    27841 acactgcatg taaacggtgg tgaaattcat gccagcgcgg aaggtcagga catgtacgct
    27901 gccattgatg gcttaattga taagctggca cgccagttga ccaaacataa agataaactg
    27961 aaacaacact aattgtccgg gcaattagca tgtgcatggc ggcctgttgt gcggcacaac
    28021 gggccatttg tacggttaat gctccgagcc tgttccactg tttgagtggg caggttctta
    28081 ggtgaaatta tgacaaataa tgatacaact ctacagctta gcagtgttct taacagggaa
    28141 tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat catcagcgag
    28201 ctggcggcga aacaacttag cctgccgcct caggtggttt ttgaagctat cctgacgcgg
    28261 gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg caaactggaa
    28321 gaagatactc tgcgcgccgt tggcgttttc gttcagctcg aaacgcctat agctttcgat
    28381 gccatcgaca accaaccggt ggacttactt tttgccctgc tggtgtcggc agaccaaact
    28441 aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa aaccatctgc
    28501 cgccgtttac gcgcagccca gagcgatgaa gagctgtatc aaatcattac ggataccgaa
    28561 ggtactccgg atgaagcgta gttattcggt aatgtctctt ttagacgttg tgaggagaaa
    28621 cagtacatgg tactgatgat cgtcagcgga cgttcaggtt caggtaaatc tgtcgccctg
    28681 cgtgcgctgg aagatatggg tttttactgc gtggataacc ttcccgtagt gttgttaccc
    28741 gatctggctc gaaccctggc cgatcgcgag atttctgccg ccgtcagcat tgatgttcgt
    28801 aatatgccgg agtcaccaga aatattcgaa caggcgatga gtaacctgcc tgacgctttc
    28861 tcaccgcaac tcctgttcct ggatgccgac cgtaatacct taattcgtcg ttacagtgac
    28921 acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc tatcgacaaa
    28981 gaaagcgatt tgctggagcc tctgcgttcg cgagcggatc tgattgtcga tacctcagaa
    29041 atgtccgttc acgagctggc tgaaatgctg cgtacccgtc tgctgggtaa acgtgaacgc
    29101 gaactgacca tggtctttga gtctttcggc ttcaaacacg gtatccctat cgatgcagat
    29161 tacgtctttg acgtgcgctt cttgccgaac ccgcactggg atccgaaact gcgtccaatg
    29221 acaggtcttg ataaacctgt cgccgcgttc ctcgaccgcc acacagaagt acacaatttt
    29281 atctaccaga cgcgaagcta tcttgagcta tggttaccta tgctggaaac caacaaccgt
    29341 agctacttga cggtcgccat tggttgtacc ggcgggaagc accgttcggt gtatattgca
    29401 gagcaactgg cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg ccatcgtacg
    29461 ctggaaaaac gtaaaccatg accgtcaagc aaactgttga aatcacaaac aagctgggca
    29521 tgcatgcccg gcctgcaatg aagctgtttg aattaatgca gggttttgac gctgaagtgc
    29581 tgttacgtaa tgacgaaggc accgaggccg aagccaacag cgttattgcg ctgctgatgt
    29641 tggattctgc caaaggacgg cagattgaag ttgaagcgac cggcccacag gaagaggaag
    29701 cactggccgc cgttatcgcc ctctttaact ctggttttga tgaagattaa tcttcatcac
    29761 tttgacatac aactcccttc aaactccccc cgacaataag aaaatcacgt actgaaatcg
    29821 ttctcaatca acgtcatttg tacattttgt gcgcttttca ctttcagaag aaccttaaga
    29881 aaaccttaag aggcattgtt taggttttgt ttaagttaat cgaccatact ggagatcgtc
    29941 agaaaatatt tccaggagat ggcatgattc gcttatctga acaaagtccc ctgggcaccg
    30001 gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc aagattgtct
    30061 accatcgtgg cgatggtggc gataaagaga tccgccgtga gttaaagtac tacgcgcatc
    30121 ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc gttgagacgg
    30181 attgcggaac cggttatgtc tacgatgtga ttgcagattt tgacggcaaa ctctccatta
    30241 cgctgaccga atttgctgaa caatgccgtt acgaagaaga cattgcacaa ctgcgccagc
    30301 tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc
    30361 cgcagaatat cctctgtcac cgcatcagtg agtcagaggt tatcccggtc gtctgcgaca
    30421 acattggtga aagcacattg attccactgg cgacctggtc gaagtggtgt tgtctgcgta
    30481 agcaagagcg gttatggaaa cgatttatcg cccaaccggc gctggctatt gccctgcaga
    30541 aagatctgca acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcca
    30601 gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt aaaatccacg
    30661 cctgacggct acgcacgtag cccgatggcg aggagacttt gaaacgaagt ggattaggta
    30721 atacagctgc cagtaatgca gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat
    30781 aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg atattcaggt
    30841 aaaccgtcag gatacgcttt ttgctccaga ctgtttctat ccccagcgtt aatccagctt
    30901 ccagcccttt tcgtacccag ctacgcccat cccataaaaa gagatttttg gccgtctgct
    30961 gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg ctcgttgtgc gccagggctt
    31021 tctcaatgga agcgacatca aagccccagt gctcagaaaa tttctgatct tctgcggcaa
    31081 tcaccgccag tcccatccac ggcgagattt gatccatact gacccagtca gaatgtgcca
    31141 cgtaacgaaa attgccatgc agccaggcgc tgacctgtcg ctcgaccatt accgctgaga
    31201 agggaacagg cgcaacacta aacaacgcga tgcccccgcc ccagaaaacg gcgaggacaa
    31261 ccattaacag caaaagaaaa cggcgaacga aactaaacac cgttaagcgg cttttactca
    31321 ttcagccaga accagcacgc gggaaaccag cttatcaatg ccgctcgccg cttctgcaat
    31381 gttctgcgcc agcatatatg ctggggtggt gacaatctta ttgtcttcat caaccacgat
    31441 atcatcgaca ggacagggca catgctccgc gcccatctct tccagcactt ctgcggtatc
    31501 gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa attttcggca gcatcgccgg
    31561 ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt
    31621 taattcacgg tcaacggtgc attcgctacc aagagtggca aaattgctta aattcttcgc
    31681 cgcgccaaac ccccccggca caatcaacgc atccagttca gcggcatcgg cctgggccag
    31741 aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc agcacgttgc gcgtttccgt
    31801 catcgcttcg ccagttaaat ggttgataac atcaacctgc tgcttatccg gtgcaaagca
    31861 gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt catgaatttc
    31921 agaaccgtca tagacgccgc atccgctcag aattacgcca attttcttca ttgtgatcat
    31981 cctttcgcaa ttgactgaaa cacattaatt tttttaataa aaatggtacg catcacacat
    32041 ttaactgatt catgtaacaa atcatttaag ttttgctatc ttaactgcgt gcggcctgaa
    32101 aaacagtgct gtgcccttgt aactcatcat aataatttac ggcgcagcca agatttccct
    32161 ggtgttggcg cagtattcgc gcaccccggt ctagccgggg tcatttttta gtggcttttg
    32221 ccacccacgc tttcagcact tctacgtcgt gacgccactc ttctttcatc tcttcaatcc
    32281 attcaccgac gttatcttcc caggcaggaa ggtcaggaga ctgaatttgc tgacccagct
    32341 gttgcagatg gcgtaacccc actgaccccg ccgcaccttt aattttatgt ccttcctcaa
    32401 caatgccttt tttatcctgc gccgtcagat tcgactccag cacgctgaca tagcccggca
    32461 tcatcttctc aaacaccgct aacccgtcgg tgatcagctt cggtcctaca agttcgagat
    32521 actgttccag catgggaata tcgagcaatg cttctgattt actgttctct tctgtcgtca
    32581 ccgtactctc ctcatcatcc tgggtatccc agaatttctt gatcatcgcg gttagcgccg
    32641 gaacagaaag cggcttactc agcacatcat ccattccagc attgaggtac tcttgtttgt
    32701 ctttcagcac gttagcggtt aaggccacca gcggcggtaa atcctctcgc ggataacgct
    32761 tcgtcagttc acgagagata tccagcccgg tcatatctgg caactgaata tccagtaaca
    32821 ccaggtcgta ttcgcccggt ttaaacatct ctagcgccgc cttgccggtc atggcgacat
    32881 caacgctgtt acctaatttt tccagcacag aacgcgcaac aatcacgttc agttcaatgt
    32941 cttccaccag cagcacattc agcgccggta aaggcatatc gtcttcatca aacgcatcat
    33001 cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct
    33061 gttcgctggt aaccgtaata tcgccgccca tatttttcgc cagacgacga gaaacggcca
    33121 gaccaatacc ggtgccggtg gcaggtttac cgccatgact gtctttcacc tggtaataca
    33181 tggcgaaaat tttatccagc tcatcctgcg gaatgccgat accagagtct tccacttcaa
    33241 aatgcagcat atcgccttca tcgtagcgca cgcgcacggt aacctggcct tgctgggtga
    33301 atttgacggc gttactgatg aggttccaca ggatctgccg taaacgcgtc ccgtcggtaa
    33361 tgacctgatg cggtaatggc agcgtcggct ccaggttaaa gcgcaatcct ttttgttgcg
    33421 cctgcaatgc ggagagattt tccagatcgg caaggaagct ggtgaaatca accggttgat
    33481 tatcaagctg gaccttgcgc cgttccatct tatccatgtc gataatatcg ttaaagatat
    33541 tccccagcgt gacggccgaa acatggatgg tcttgagata tttttcctgc tcggcggtga
    33601 gttcggtatc cagcagaatg cggctcagac cgacgatacc gttcagcggt gtacgcaatt
    33661 cgtgactgat ggtggagata aacgtcgttt tgtcgcggct ggcccgttca agcgcatcct
    33721 gataccgctt acgctcggta atgtctcgac caaagcccat caaaccgtga cgtttaccca
    33781 cgcggtcgta gtacggcact ttacggattt caaagcaggc tttgcgcccg tccgggtaat
    33841 ccagccactg ttcataggtc agtgacacat tatgacggaa cactttttca tcggtttcaa
    33901 tgacttttgc ggcggcttcc ggtgagtaaa catcaacagg tttcaggtga accagttgtt
    33961 tttcgctttt tccggtcagc agctccatcg cgcggttaca gccggaaaac tctttatctt
    34021 cgttacgata aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaag aatgaggatt
    34081 gctgctcgag ctgaatttgt gtctcttcgc gctctttgat ttcaattttc agttggccga
    34141 aggtttcctg cagttctgct tccgcttttt cacgaacggc gatttcctga tttagctggg
    34201 caatattatc ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt
    34261 tttgcaccag ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg accaccgata
    34321 gaaagtagac cgcccacggc gtaatcaaca aaccaaagaa gatagaacga ataacatcaa
    34381 tgctttcgac ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca atggcaagaa
    34441 cgacgagggc cagcgccagc aacattgaga agcgcaccag acctaacttc atcatcaggt
    34501 caacataata ctgcgccagc agacgaattt gcttcattag ggaattcctt cacgacaacc
    34561 tgccccaata atacccaatt ctgacagtta cgttataaat tgtgtgagaa atgcggaatt
    34621 cccctcaacg acctactccg ttggaggtag ccacggacgt cccagcgccg atccctgtac
    34681 gccatgttca ttcagatagc gatccagctc gaccattccc gtccatcgat tttcgcacca
    34741 cagaggagca agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt agatcacttc
    34801 cggtggcgta tggcgaatca tttctccggc agtgagcgtg taatcctcca gttcaatacc
    34861 gttcaaacgc cccgcttccc aggctttcgc cataatgctg cctttcacaa tatgcaacgg
    34921 atgtagcttt ataccgtcaa caccggtttc cactacccgc tccagcgttt gcagacattc
    34981 agccagccct tcgcctggca gaccgacaat taaatgcgag cacaccttca gcccgcgctg
    35041 gcgtgccagc tgcgtcgtgc gctgataaca ggcgaaatca tgaccgcgat taatgcgatg
    35101 tagcgttttg tcgtgcacgg tttgcagccc cagctccagc cacacttcgt agccctggtc
    35161 tttatattcg caaagcagat ccagcaccgc atccggcacg cagtccgggc gcgtgccgac
    35221 acacaaaccg acaatattgg cctggctcac cgcctgttga tacatcgaac gcagcacctg
    35281 aacttcggca aaggtgctgg tatacgcctg gaaataggcg aggtaacgtt tcgcgcgatt
    35341 aattaaattc gcctgatgcg ccagctgttc ggcaatggaa cgatgctgct gcgcttcgtc
    35401 ggcaaacgag gcaacattac agaaggtgca gccgccgcgc ccgatggtgc cgtcgcgatt
    35461 cgggcaactg aaaccgccgt gcagcgtcag cttatgcacc ttttgcccat aacgacgggt
    35521 gagatcacca ccaaacatat tgactaattt ctgtaactgc ataatctgat agaccgcgcc
    35581 ttgaaaagag gccaaagcct gccattttta gctcaattcg gcgatgacct ggatcaatcg
    35641 tcccggcccg cttttatcaa ctgcataatc aatcaaaatt accgaaattt catgcataat
    35701 cacataaatc acttttgctt atcttgtgtc agattttttt atctccttat ggattttagg
    35761 caaaaacagt agcatgaaac gtcattacca attaatgcag tataaaatgc tggttttctc
    35821 gtcaattcaa ggcaggataa gggttaacac gcctttatga cagtcaggaa ttgactgttt
    35881 ctctaacgac ttccctttta gccttaaaga taaaatctat tttaatttca gccatttaat
    35941 aaagaatttt gcgctaaagc acatttctgt accaataagc ttgccatttg acctgtatca
    36001 gctttcccga taagttggaa atccgctgga agctttctgg atgagcagcc tgctcatcat
    36061 atttatgcag taattgagat cccctcttca ccgtattaac cgatgcgaaa aggacaacaa
    36121 gggggcgaat gcgaggcgcg cgtatgacac gcaacccccg tcgccacgct ctttctgtac
    36181 ccgtgcgcag cggttcggta gtggggttcc cgcagagcct gggggaggtt cacgatatgt
    36241 tgtacgataa atcccttgag agggataact gtggtttcgg cctgatcgcc cacatagaag
    36301 gcgaacctag ccacaaggta gtgcgtactg caatacacgc actggcccgc atgcagcacc
    36361 gtggcgcgat tctcgccgat ggtaaaaccg gtgacggttg cggcttgctg ttacaaaaac
    36421 cggatcgctt ttttcgcatc gttgcgcagg agcgcggctg gcgtttagcg aaaaactacg
    36481 ccgtcgggat gctattcctg aataaagatc ctgaactcgc cgctgccgca cgccgcatcg
    36541 ttgaagaaga actgcaacgc gaaaccttgt cgattgtggg ctggcgtgat gtccccacta
    36601 acgaaggcgt actgggtgaa atcgccctct cctctctgcc ccgcattgag caaatttttg
    36661 tgaacgcccc ggcaggctgg cgtccgcgcg atatggagcg ccgtctgttt atcgcccgcc
    36721 gccgcattga aaagcgtctc gaagccgaca aagacttcta cgtctgtagc ctatcgaatc
    36781 tggtgaacat ctataaaggt ctgtgtatgc cggcggatct gccgcgcttt tatctggatc
    36841 ttgcggacct gcgtctggaa tcggccattt gcctgttcca ccagcgcttc tccactaaca
    36901 ccgtaccgcg ctggccgctg gcgcaaccgt tccgctatct ggcgcataac ggtgaaatca
    36961 acaccatcac tggtaaccgc caatgggcgc gtgcgcgtac ctataaattc cagacgccgc
    37021 ttatccctga tctgcacgac gccgcaccgt tcgtcaacga aaccggctct gactccagtt
    37081 cgatggataa catgctggaa ctcctgctgg caggcgggat ggatatcatc cgcgccatgc
    37141 gtctgttagt gccacccgcc tggcagaaca acccggatat ggacccggaa ctgcgtgcct
    37201 tctttgactt taactccatg catatggagc cgtgggatgg cccggcgggc atcgtgatgt
    37261 ccgacggtcg ttttgccgcc tgtaacctcg accgtaacgg tctgcgtccg gcgcgctacg
    37321 tcatcaccaa agataagctc atcacctgcg cctctgaagt cggtatctgg gattaccagc
    37381 ctgacgaagt ggtcgaaaaa ggccgcgtcg ggccaggcga actgatggtt atcgacaccc
    37441 gcagtgggca tattctgcac tcggcagaaa ccgatgacga tctgaaaagc cgccatccat
    37501 ataaagagtg gatggagaaa aacgtccgcc gactggtacc gtttgaagat ctgcccgatg
    37561 aagaagtggg tagccgcgaa ctggacgacg acacgcttgc cagctaccag aaacagttta
    37621 actacagcgc ggaagagctg gactccgtaa ttcgcgtact gggcgaaaac ggtcaggaag
    37681 cggtcggttc gatgggcgat gataccccat tcgccgtgct ctccagtcag ccgcgcatta
    37741 tttacgacta cttccgccag cagtttgccc aggtgactaa cccgccaatc gacccgctgc
    37801 gtgaagcgca tgttatgtcg ctcgccacca gtatcggtcg tgaaatgaac gtcttttgcg
    37861 aagcagaggg ccaggcgcac cgtttaagct ttaaatcgcc gattctgctc tactccgatt
    37921 tcaaacagct cacgacgatg aaagaggagc actaccgcgc agatacgctg gatatcacct
    37981 ttgacgtcac taaaaccacg ctcgaagcga cagtcaaaga gctgtgcgac aaagccgaaa
    38041 aaatggtacg tagcggcacc gtgctgctgg tgctctccga ccggaatatc gctaaagatc
    38101 gcctgccggt tccagccccg atggcggttg gcgcgatcca gacccgtctg gtcgatcaaa
    38161 gcctgcgttg cgatgccaac atcatcgtcg aaaccgccag cgcccgcgat ccgcaccact
    38221 tcgccgtgtt gctgggcttc ggcgcgacgg ctatttatcc ataccttgcc tatgaaacgc
    38281 tgggccgcct ggtagacacc catgcgattg ccaaagatta tcgtaccgtg atgctcaact
    38341 accgtaacgg catcaacaaa ggcttgtaca aaatcatgtc caaaatgggc atctccacca
    38401 tcgcctctta ccgctgctcg aaactgtttg aagcggtcgg tctacacgat gatgtagtgg
    38461 gcctgtgctt ccagggggcg gtcagccgca ttggtggggc aagctttgaa gacttccagc
    38521 aggatctgct gaatctgtcg aaacgtgcct ggctggcgcg taagcccatc agccagggcg
    38581 gcctgctgaa atacgtccac ggcggcgaat accacgccta caacccggac gtggtgcgca
    38641 cgctgcaaca agcggtacaa agcggcgagt acagcgacta tcaggaatac gcgaagctgg
    38701 ttaatgagcg tccggcaacc acgctgcgcg atctgctggc aattacgccg ggtgaaaacg
    38761 cggtcaacat tgctgatgtt gaaccggcaa gcgaactgtt taaacgcttc gataccgctg
    38821 cgatgtctat cggcgcgtta agcccggaag cccacgaggc gctggcggaa gcgatgaaca
    38881 gcatcggcgg taactcaaac tccggtgaag gcggtgaaga tccggcgcgc tacggcacca
    38941 acaaagtgtc gcgcatcaag caggtggctt ccggtcgctt tggggttact ccggcgtatc
    39001 tggtcaatgc cgacgtcatt cagattaaag tcgcccaggg cgcgaagccg ggcgaaggcg
    39061 gtcagttgcc gggtgataaa gtcactcctt acatcgccaa actgcgctat tcggtgcccg
    39121 gagtgacgct gatctccccg ccgccgcacc acgatatcta ctctatcgag gacttagcgc
    39181 agctcatttt cgacctcaag caggttaacc cgaaagcgat gatctccgtg aagctggttt
    39241 ccgaaccggg cgtaggcacc atcgcgactg gcgtggcaaa agcttatgcc gatttaatca
    39301 ccatcgcagg ctatgacggt ggcaccggcg caagcccgct gtcgtcagtg aaatacgcag
    39361 gttgcccgtg ggagctgggg cttgttgaaa cccagcaggc gctggttgcc aatggtctgc
    39421 gtcataagat ccgtttgcag gtcgatggcg gcctgaaaac cggtgttgat atcatcaaag
    39481 cggcgattct cggcgcagaa agcttcggct tcggcactgg cccaatggtc gcgctcggct
    39541 gtaaatatct gcgtatttgc cacctgaaca actgcgcaac gggtgtggca actcaggatg
    39601 acaaactgcg taagaatcac tatcacggcc tgccgttcaa ggtgacgaat tactttgagt
    39661 ttatcgcccg tgaaacccgc gagctgatgg cacagctggg ggtaacgcgg ctggtggatc
    39721 tgattggtcg caccgacctg ctgaaagagc tggacggttt caccgccaaa cagcagaaac
    39781 tggcgctgtc gaagctgctg gagaccgccg aaccgcatcc aggtaaggca ctctactgca
    39841 ccgaaaacaa cccgccgttt gataacggcc tgctgaacgc gcagttgctg caacaggcga
    39901 aaccgtttgt cgatgaacgc cagagcaaaa ccttctggtt cgatatccgc aataccgatc
    39961 gctccgtggg cgcgtcgctt tcaggctata tcgcccagac gcacggcgat caggggctgg
    40021 cagccgatcc tatcaaagcg tacttcaacg gcaccgcagg ccagagtttc ggtgtatgga
    40081 acgcgggcgg cgtggaactg tacctgaccg gggatgccaa cgactatgtc ggtaaaggca
    40141 tggcgggcgg cttaatcgcc attcgtcctc cggttggttc cgccttccgc agccatgaag
    40201 caagtattat cggcaacacc tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag
    40261 gccgcgcggg tgaacgtttc ggcgtgcgga actccggtgc tatcaccgtg gtagaaggca
    40321 ttggcgacaa cggctgtgaa tatatgacgg gcggtattgt ctgtattctg ggtaaaaccg
    40381 gcgttaactt cggtgcgggc atgaccggcg gcttcgctta cgttctcgat gaaagcggtg
    40441 atttccgcaa acgcgttaac ccggaactgg tcgaggtctt aagcgttgac gatctggcga
    40501 tccatgaaga gcatctgcgc ggtcttatca ccgagcatgt tcagcatact ggctctcagc
    40561 gcggtgaaga gattctggcg aactggtcaa ccttcgccac taaatttgcg ctggttaaac
    40621 cgaagtccag tgatgtaaaa gcactgctgg gtcatcgtag tcgtagcgca gcagagttgc
    40681 gcgtgcaggc gcagtaaggg gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc
    40741 tgcagcgcgt tgatccgcca aagaaaccgc tgaagatccg caaaattgag tttgttgaaa
    40801 tttacgagcc gttttccgaa ggccaggcca aagcgcaggc tgaccgctgc ctgtcgtgcg
    40861 gcaacccata ctgcgagtgg aaatgtccgg tacacaacta catcccgaac tggctgaagc
    40921 tcgccaacga ggggcgtatt tttgaagcgg cggaactgtc gcaccagacc aacaccctgc
    40981 cggaagtttg cggacgagtc tgcccgcaag accgtctgtg cgaaggttcc tgcactctga
    41041 acgatgagtt tggcgcggtg accatcggca acattgagcg ttatatcaat gataaagcgt
    41101 tcgaaatggg ctggcgtccg gatatgtccg gcgtgaagca aaccggtaaa aaagtggcga
    41161 ttatcggcgc aggcccggca ggtctggcgt gtgcggatgt cctgacgcgt aacggcgtaa
    41221 aagccgttgt cttcgaccgt catccagaaa ttggcgggct gctgaccttc ggtattccgg
    41281 ccttcaagct ggaaaaagag gtaatgacgc gtcgccgtga aatcttcacc ggcatgggta
    41341 ttgaattcaa actcaatacc gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg
    41401 attacgatgc cgtcttcctt ggcgtcggga cttatcagtc aatgcgcggc gggctggaaa
    41461 acgaagacgc cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac accaaacagt
    41521 taatgggctt tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg
    41581 tggtccttgg cggtggcgac actgcgatgg actgcgtgcg tacgtccgtg cgccagggag
    41641 cgaagcacgt tacctgtgcc tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg
    41701 aagtgaaaaa cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag ccactgggga
    41761 ttgaagtgaa cggtaacggc aaagtcagcg gcgtaaaaat ggtacgtacc gaaatgggcg
    41821 aaccggatgc caaaggccgt cgccgcgcgg agatcgttgc aggttccgaa catatcgttc
    41881 cggcagatgc agtgatcatg gcgtttggtt tccgtccaca caacatggaa tggctggcaa
    41941 aacacagcgt cgagctggat tcacaaggcc gcatcatcgc cccggaaggc agcgacaacg
    42001 ccttccagac cagcaacccg aaaatctttg ctggcggcga tatcgtccgt ggttccgatc
    42061 tggtggtaac cgctattgcc gaaggtcgta aggcggcaga cggtattatg aactggctgg
    42121 aagtttaagc tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg aaattctcct
    42181 ttctttctgg aaagcgcctc gcataatctg aagaaaacca atgtaatacg ctcatctgga
    42241 ttgttaccgg tagatgcccg gcgtatagct gattccatta ttcaacgcag cgccatataa
    42301 agggacagat aatggaatcg ctctcagaga gaacctcaac aggctaccag caaatccacg
    42361 acggtattat tcacctggtc gatagcgccc ggacggaaac ggtacgtagc gttaacgcgc
    42421 tgatgaccgc gacatactgg gaaattggcc gacgaattgt cgaatttgaa caaggtggcg
    42481 aggcaagggc tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat ttaagtctaa
    42541 ggtataagcg tggtttctct gcaaaaaact tacgccaaat gaggcttttt tacctctttt
    42601 ttcaacatgt tgaaattcgc cagacagtgt ctggcgaatt aacaccattg ggaattccgc
    42661 agacaccatc tgcagaattc ccatcggcaa agatttggca gacgctgtct gccaaattga
    42721 gaggctgttt gatatttcca cagcttttgt attgcatctc ctattttcgg tttgtatatt
    42781 catgcaaata aacgaaggaa agttgccgtg atttacattg aacagcttga attaatccat
    42841 aaaagtggtg atgtgttgta tccagtaaaa ataaccagaa aatcatcagg aaaaactgcc
    42901 ttccaccttg ttccattcgg cttaaataag actcacgacc ttctagaggt ggaagatgct
    42961 tcagaagcaa ttcggctggt gattgatgaa cgtcattcca ttcgctgcag tacacttaca
    43021 gcaactatta cgaataaaaa gggcaagcga attaagcgga caggtatata cagtatcaag
    43081 ggtgttaaca taaaagaata caacgtcagg taatggcaca gagcggtccg tcagttcagg
    43141 tttggctctg tgccaaagat gggtcaggtc acacctgatc taataaattt aagccattaa
    43201 atcagcctta accttcacca ccaccttttt aatctcaccc ggctcgccaa caacgcatcc
    43261 cggcttatgc ggctctccta gcataaacac ggcgaacatt cccggtttca ggatgatggc
    43321 ttgctcgttt tcaatggtgc tgcaaagctg gtaatcatcc tcatggtgga actcttcaca
    43381 ctgacgcgca gtgcctgcca tgccaaacag aatccgttct tcaccgttta ataacagctg
    43441 gatatcaatg tattgctcgt gcagctccgc ttttttctca acgggcgatt gagtgttaaa
    43501 cgtcatgaca ttcataaaaa tattgtcgcc ctgtaattcg taacgacccg gcgctttttc
    43561 ttgcggtctg gcagctaatg ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc
    43621 agacggtaat gaccgtactt cacccatcat cataattttt ctccctgggc caacagcgct
    43681 gccccaagta aacctgcatc atggcggtag tgcgccgcca gtaaatcaac atgaaatacc
    43741 gctggctcct gcgccagata cgtttccacc aacgccagat acccttctgc cagaccaacg
    43801 ctgccaccga ccaccacgca ctggcaatca gttgtggctt taatatcagc gatcagcctt
    43861 gccagcacat gtgcggagcg gtgaatcagt tgctgcgcct gctcgtcgcc ctgcccggcg
    43921 cgtgtgaaaa tagttctcgc atccgcgccc atgagttcac cttgcgctgc cgctgcaata
    43981 ccgcgaccag aagcaatcgc ttccacgcaa cctgtgcgtc cacagccgca gactgggccg
    44041 tgtggatcgg caagcgtatg cccgatatgc cccgccagac cgccagggcc ggtaagcagt
    44101 ttaccgccgc tcactacacc gccgccgacg ccagtggaaa cggtgataaa caccatctcg
    44161 gttacatcgc cttccagcgc ctgatactct gcccatgctg cggcctgcgc gtcgttaatg
    44221 gcaatggtcg gcaaattggt aagttgttcc agcgttttga ctaacggaaa gtgtagcaat
    44281 ccaccgagat tatgcggatt cagcgccagc aaactgccgt cgcgaataat tccggttgaa
    44341 gcgatggcaa cccgctgcgc atgagcttgc aacggagaga ctaatgcgga taaggcatca
    44401 cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt cacgacgatc gcggatctgc
    44461 ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg
    44521 gtcatagcac cgcctttttc atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg
    44581 taatggcaga accgaccgtc accgcccacg cgccgtggcg catcgcatct gccgcctgag
    44641 caggcgtgtt gtaacgccct tcggcaatca cccgacatcc ggcttcgctc aacgttttca
    44701 ccagcgccag atccggctct tctggcgttt caggcgtggt atagccagaa agcgtagtgc
    44761 caataatttc ggctcccagc ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg
    44821 tcatcgccag taaaccgtga tgatgtatac gtgccagcag cgtttcaaca ggcaccggac
    44881 gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga
    44941 catcttcaat ataggccgtg atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc
    45001 caataatcgg cacgctcacc accgcacgcg tggcttgcag atttgccaca ccttcgatgc
    45061 gaatggcaac cgcgcccgcc tgctctgccg ctaatgccat ggctgcgacg atttcgggtt
    45121 tatcgagcgg gctgtcagga atcggctggc aggagacaat caggccaccg ttagcagcga
    45181 ttttttgatc cagttgtgca agtaacgaca tacatcttcc cttagcgaaa ggcccggtac
    45241 atagaccggg caacaggatt aacttttggt tttgactaaa tcgtttttgg cgctgccaaa
    45301 tggcacggca ccgctaaatg gtttgccgtc gatagcgtca tgagtacgca atgcttccgg
    45361 gcgcagccaa cgctgaacgc gggaaggcat atccagacca atcagcagga tcaccacaaa
    45421 tgtcaggctg aacgagagcg atgccagcgc agtacccaga tccagacgtt gagcgatcaa
    45481 cgcgccgatg attggggcca gtgcgccacc caatgcgcca acgttgtagg taaagcccag
    45541 gcccgctgca cgctggtcgg tatcgaaata accgccaatc agtttcggta agatcccggc
    45601 gatcccttgt ccaagcattt gctggaagaa cagtaacaga ccgagcaccc agacgtttgc
    45661 gccgccaatc gcaaataccg gaataatcag cagctgcgag gccagcaggc tacaaacgta
    45721 cgctttgcgg gttcccagcc agtcaccgag gaagcctcct acgcagcatc ccacggccgc
    45781 gccaaagcca ctaaagaaca gcacattggc gacagtatgc gggttataag ccagatcggt
    45841 tttcagatac gttggcagca gcgcctgaat cggccatgag tagaggaaag caaacaacac
    45901 gaccaccatc agcattacgc ccgttggcca gcgtttgcct gtactctgca ccataaagct
    45961 gataaagatt gcggcgcata acagcccaag aacagcgacg atcgcggcat tttgcaggtt
    46021 accggcgaag cagaaccaca gcgcagtagc cgccgccagt gtcattacga tattggcaat
    46081 gcgatgttca ccacggtaga gaatatccac cattgtgcgt actggtgctt tacctgcgtg
    46141 tttctctttc cagtcttccg cttccgggat gtttttacgc agccagagag caaagatgat
    46201 tggcaaaatg ccgataaaga acagcgcacg ccaaccccag accggaacca ccaggctata
    46261 gacctgagcg gcaacgacgg cccccacaga gaagcctgaa atcaaaaaac cactggcttt
    46321 gttacgcaga tgttttggcc agctttcaat gacataggtg gcgctggaac cgtattcacc
    46381 cgccatcccc atgccgatga ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa
    46441 gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg gtgaccattg ccagacgacg
    46501 cccgtagcgg tcacccatag cgccgagcat caggccgccg aaccagcgag agataaaggc
    46561 tgcggagatc agacttgccg cctgcaccgt cgtcagcccg aattcaccct gtacttcggt
    46621 gagtaccagg gcgattaaaa cgaaatcaaa accgtcaagc agatatccca accaggcagc
    46681 ggaaaatgcg cgccattgtg cacggttgag atggcgatac cacgggatat tctgggttgt
    46741 agtactcatt gtgagtctcc cgcggtgggc gatgcccaca cgctttggta tgaaaattgt
    46801 agggtacaga tgcgtttatt tcccctcacc cggtaggggc gagcgagggg aaaacaactc
    46861 acccgcgctc ttgcatcaac tgctgggcca gcgccttcag ttctggcaga tatttttcat
    46921 ctaccggtcc aaacggtttg cggcacagcg gcacagaaac gacatccata taatgcagga
    46981 cagttttcag gccgcggaat acgccagttt tgatcagtaa atcaatgact ttgttgcact
    47041 cagtttgcag tttctgcgcg gtctggatat cgccttcttt cagcgccttc acgatcccct
    47101 gatatcgcca gcccatgatg ttgtaagtac tgccgatacc accatcagcg cctgccagca
    47161 agccggaagc gaagatttcg tcgtaaccgt tatagagcac cagatcagga tgttcacgac
    47221 ggatctgttc catctgatag agatcgccag aagtctgttt cagcgcaccg acgccaggca
    47281 acgtaaccag ggtgttgatc tgatccaggg tcagttttac cccactcagg gctggaatgt
    47341 tgtacacaac catcggcaaa ccatccgctg aatcaataat tgcccgatag tgatcgcagt
    47401 gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc ggagacggca tcgaagccat
    47461 aacgtttagc cgatgccgca agttgttggc tttcggcggt gctgacgcaa ccgacgtggg
    47521 cgatgagttt aatcttacct ttcgcctctt cggcgacgat ttccagtacc tgttcacgct
    47581 cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc cacgtataaa ccgtcgatgc
    47641 cctgctgaat attgaactga accaggcgac gcagactcgc tttatccagt gcttgttgtt
    47701 ggtcaaaagg agtcaggagt gcagccatta cgccacgtaa attcgttgcc ataaatacct
    47761 ctgaagtgat acttgtctga taaacgatat acctttatac ctgttatacc agatcaatta
    47821 agcaacaccc catacagaaa gcttataatg cgatctgctt cactaaagtg gcattatttc
    47881 tttttgttgg tggtctgacc gaaagcgtgc caggtagcag agacgctgtt gagatgcgat
    47941 tgcaacgcac gatcggcttc gtcaggatca tgacggcgga tcgcatcaac gatcgcaata
    48001 tgctgttgat agctaacgtt gttatgctcg tgcagagcct gatcagccac ggttggacgt
    48061 gcggcgatca gccagtcgag cagggcaacg tggatcgcca tgaagattgg gttaccaggg
    48121 atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc
    48181 agcgactgac tgttgatttc cagtgctttt gccagcaaat cgatttgctc atcggtggca
    48241 tgttcagccg catagcgcac cagactggat tcaaagaaca gacgtaattg ttcgaaatgg
    48301 gcaatcccac cgggatgaga aaggaaatct ttcgccatgc cggaaaactc accgatgata
    48361 gtgtccgcag aaggacgcga gacgcgagcg cgttcgccgt tgtttatttg caccagacct
    48421 ttgcgtttta acgctgccag cgcttcgcgc acagaaggac gcccgacgtt aaagaacgcc
    48481 atcagttcgc gttcagacgg taattgttca ccttcgccaa attcacgacg gcggatcatc
    48541 tgttccagct cttcttccac catttcggag agttttttac gcgccagcgg gcggctacgc
    48601 aagttgcgac caattgcagg tgaagaatct tcggtttgcg aatcaaatgc gttcataagg
    48661 cccattctgt aaggtcagtg tgattaacat catcagtgac atcctatcac aggattgaaa
    48721 gtaggggaaa atggcaggtt tttctctttg tgcctcatca ttaccataat taacggaata
    48781 attaactatt gcgaaaaatt aatgtaacgc agataaacac atcccgattg aattattcat
    48841 aagactattc acaagcatta tgaatattat gaatgtgttc ttacaaaata atcataagcg
    48901 catatttttt aatgaaaaat cacatcacct acaattaaaa acacgacatc cgcaccataa
    48961 atagacttgc aaaaaatata acatcattgt tttcaatctg ccgtttatgg gattgaccgt
    49021 tttcttttga cacggagttc aacaatgttc ggcataatta tatctgtcat cgtattaatt
    49081 acgatgggct atttgatcct gaaaaactac aaacctcagg tggtgctggc tgccgcaggt
    49141 gtcttcctga tgatgtgcgg agtctggtta gggttcggtg gtgtactcga tcccgccaaa
    49201 agcagcggct acttgatcgt cgacatttat aatgaaatcc tgcgcatgct gtccaaccgc
    49261 attgccggat tggggctgtc gattatggcg gtgggcggtt atgcccgcta catggagcgc
    49321 ataggagcca gtcgcgcgat ggtgagcttg ctaagccgcc cgttaaaact cattcgctcg
    49381 ccgtatatta ttctgtcggc aacttacgtc atcggccaaa tcatggcgca gtttattacc
    49441 agcgcctccg gtctgggtat gttgctgatg gtcaccttat ttccgacgct ggtgagtctg
    49501 ggagtaagtc gcctctctgc ggtggcggtt atcgcaacca cgatgtccat tgagtggggg
    49561 attctggaaa cgaactccat ttttgcagcc caggtcgcgg gaatgaaaat tgccacatac
    49621 ttcttccact accagcttcc ggtcgcctct tgcgtcatta tctcggtggc gatctcccac
    49681 tttttcgtgc aacgcgcttt tgacaaaaaa gataaaaata tcaatcacga acaggcagag
    49741 caaaaaactc tcgataatgt cccgccgctc tattacgcca ttttacctgt gatgccgtta
    49801 atcctgatgc tcggctcgct gttcctcgcc cacgtcgggc tgatgaagtc agaactgcat
    49861 ctggtggtgg tgatgttact aagtttgacg gtgacgatgt ttgttgagtt cttccgcaag
    49921 cataacttgc gcgaaacaat ggacgatgtg caggcgtttt ttgacggcat gggtacgcag
    49981 tttgccaacg tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg
    50041 attggcactg ttgatgcggt tatcaggggt gcggagcatt ctggtctggg cggtattggc
    50101 gtgatgatta ttatggcgct agtcattgcc atttgtgcca ttgtgatggg ctctggcaat
    50161 gcgccgttta tgtcatttgc cagtcttatt ccgaatatcg cagccggact acatgtacca
    50221 gcggttgtaa tgattatgcc gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg
    50281 attactgcgg tggtgaagcg gacagcgatc cccatggcag tcggtttcgt ggtgaatatg
    50341 attgccacaa tcacgctatt ttattaagtc attaaaaaga caaaacaggc cgcctgggcc
    50401 tgttttgtat tacttcacaa cacgtaatgc cggtcgacca ccgcgtggtg gctgcggagg
    50461 ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat
    50521 aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggtat cttcatcgta
    50581 ggcagcttca ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga tagccagcac
    50641 ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac
    50701 ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg
    50761 cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag
    50821 gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg
    50881 acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg catttcacgt
    50941 tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct
    51001 ttcagctctt tcgcacccgg gccgctgagc tcgataccca gttgcggcag acgccacagc
    51061 agcggagcaa gatagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga
    51121 ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct
    51181 tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga
    51241 tgcatgtaca gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc
    51301 gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt cagctcacga
    51361 tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg
    51421 tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc
    51481 tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca
    51541 gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt
    51601 ggccagtcag aagttgtgtt acccaataag gaacgactct ctttgttcga aaatcaaaca
    51661 aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc
    51721 gaccattgtg gtgagtggat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat
    51781 agttgactta gacccttatt ggcgatgtgg tttttgtttt gcctgtcttt cagacggcag
    51841 caaaaagcaa cttcccattt tttacgctga ttcagaattt agccataaaa aaacccgccg
    51901 aagcgggttt tttcgtaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa
    51961 ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc
    52021 acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag
    52081 agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac
    52141 ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac
    52201 catgcgggca gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt
    52261 accgttgccc ggtttgatga aaacgcgagc tgcggaattt ttgcggcgac cagtgccgta
    52321 gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg
    52381 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
    52441 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
    52501 agcaatcatc tcttcaaagc tcacttgttt gataccaccg atgtggccgg tgtggtgata
    52561 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
    52621 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgtaggcg
    52681 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
    52741 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
    52801 atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
    52861 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
    52921 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
    52981 tttgtgattt gtgacaggtt ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt
    53041 tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg atcgccctga
    53101 taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
    53161 tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt
    53221 acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc atgctgactg
    53281 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
    53341 gtcgctaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt cccccccgcc
    53401 agcgccaacc atagtttatc catttgcgcc cgggtttcat cgtggagtgg cgaaagccac
    53461 agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca
    53521 tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caagccattt
    53581 cgataaagtt cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac
    53641 aaggctttca tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg
    53701 tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg caataatttc cagcggatcg
    53761 gtctgcccct gtaaggcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc
    53821 aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
    53881 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg
    53941 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc
    54001 gtgctattga tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg
    54061 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg
    54121 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
    54181 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa
    54241 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
    54301 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
    54361 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
    54421 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
    54481 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
    54541 tatcagcaca tggcgaaaag ttccagcagc ctgctgccgg aactgtctgc tgaagcaaac
    54601 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg
    54661 cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa
    54721 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
    54781 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
    54841 ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaacccca gctgttgagt
    54901 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt
    54961 ccaggccagg ttgccgatca ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg
    55021 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
    55081 gaagaattca aaaagttttt tggtgatgat ttaccggagc aacctgcaca acccttcgaa
    55141 ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat
    55201 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
    55261 aaactgattg gtagcgatga ccagagcgat atcgccctgt tacaaattca aaacccgagc
    55321 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgta
    55381 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
    55441 ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
    55501 aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat tggcatcaac
    55561 actgcaatcc ttgcgcctgg cggcgggagc gtcgggattg gatttgccat ccccagtaat
    55621 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta
    55681 ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
    55741 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa
    55801 gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
    55861 tctcgtatcg cgaccaccga gccgggcaca aaagtgaaac ttggcctgct gcgtaacggc
    55921 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
    55981 atgatcgcgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
    56041 aaaggtatta agatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa
    56101 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
    56161 aaagtgctgg cggcaaaatc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
    56221 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg
    56281 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
    56341 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
    56401 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
    56461 ctgccagcta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc
    56521 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa
    56581 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
    56641 tcatcgtcgc cttacaggat ggtcgtgtat ttgaagcatt gctggtggga tctgactctc
    56701 taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc attccaatca
    56761 atgcacgtcg cgtaccgcat attggcgacg tggtactggc gatcggtaac ccgtacaacc
    56821 tcgggcagac cattacccag gggattatta gtgccacggg tcgaattggt ctgaacccga
    56881 ccgggcggca aaacttcctg caaaccgatg cttccattaa ccacggtaac tctggcggcg
    56941 cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta
    57001 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa
    57061 ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggc attggcggac
    57121 gtgagatcgc accgctgcac gcacagggcg gtggtataga tcaactgcaa gggatcgtgg
    57181 ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aacgatctga
    57241 ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcgg
    57301 aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc
    57361 tgcaggtcac cattcaggaa tatccggcaa ccaattaagt cgagtgctca aaacaaaaaa
    57421 ccggagtctg tg
//
LOCUS       DAJTVK010000012        55736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000012 DAJTVK010000000
VERSION     DAJTVK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.012"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="OO372_000778"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="OO372_000778"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6340362.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO372_000779"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="OO372_000779"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6340363.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO372_000780"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="OO372_000780"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6340364.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO372_000781"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="OO372_000781"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6340365.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO372_000782"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="OO372_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6340366.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO372_000783"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="OO372_000783"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6340367.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="OO372_000784"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="OO372_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6340368.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO372_000785"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="OO372_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6340369.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="OO372_000786"
     CDS             6537..6773
                     /locus_tag="OO372_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6340370.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="OO372_000787"
     CDS             6776..7633
                     /locus_tag="OO372_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6340371.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="OO372_000788"
     CDS             7654..9645
                     /locus_tag="OO372_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6340372.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO372_000789"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="OO372_000789"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6340373.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="OO372_000790"
     CDS             complement(10248..11144)
                     /locus_tag="OO372_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6340374.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="OO372_000791"
     CDS             complement(11193..11432)
                     /locus_tag="OO372_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6340375.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="OO372_000792"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="OO372_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6340376.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="OO372_000793"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="OO372_000793"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6340377.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="OO372_000794"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="OO372_000794"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6340378.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="OO372_000795"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="OO372_000795"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6340379.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="OO372_000796"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="OO372_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO372_000797"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="OO372_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6340380.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="OO372_000798"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="OO372_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6340381.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="OO372_000799"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="OO372_000799"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6340382.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="OO372_000800"
     CDS             complement(21570..22739)
                     /locus_tag="OO372_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6340383.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO372_000801"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="OO372_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6340384.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="OO372_000802"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="OO372_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6340385.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="OO372_000803"
     CDS             complement(24315..25247)
                     /locus_tag="OO372_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6340386.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPIHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="OO372_000804"
     CDS             25360..26571
                     /locus_tag="OO372_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6340387.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="OO372_000805"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="OO372_000805"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6340388.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO372_000806"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="OO372_000806"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6340389.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="OO372_000807"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="OO372_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6340390.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="OO372_000808"
     CDS             complement(30320..31576)
                     /locus_tag="OO372_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6340391.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="OO372_000809"
     tRNA            31884..31960
                     /locus_tag="OO372_000809"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="OO372_000810"
     tRNA            31965..32041
                     /locus_tag="OO372_000810"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="OO372_000811"
     CDS             32149..32454
                     /locus_tag="OO372_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6340392.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="OO372_000812"
     CDS             32580..34184
                     /locus_tag="OO372_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCR6340393.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="OO372_000813"
     CDS             complement(34196..35008)
                     /locus_tag="OO372_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340394.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="OO372_000814"
     CDS             complement(35012..35797)
                     /locus_tag="OO372_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6340395.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="OO372_000815"
     CDS             complement(35794..36462)
                     /locus_tag="OO372_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6340396.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="OO372_000816"
     CDS             complement(36526..37164)
                     /locus_tag="OO372_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6340397.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39285)
                     /locus_tag="OO372_000817"
     CDS             complement(37177..39285)
                     /locus_tag="OO372_000817"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR6340398.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="OO372_000818"
     CDS             complement(39306..39932)
                     /locus_tag="OO372_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6340399.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO372_000819"
     CDS             complement(40388..40597)
                     /gene="fumD"
                     /locus_tag="OO372_000819"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6340400.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            41154..42566
                     /gene="pykF"
                     /locus_tag="OO372_000820"
     CDS             41154..42566
                     /gene="pykF"
                     /locus_tag="OO372_000820"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6340401.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42876..43112
                     /gene="lpp"
                     /locus_tag="OO372_000821"
     CDS             42876..43112
                     /gene="lpp"
                     /locus_tag="OO372_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6340402.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO372_000822"
     CDS             complement(43175..44179)
                     /gene="ldtE"
                     /locus_tag="OO372_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6340403.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO372_000823"
     CDS             complement(44328..44744)
                     /gene="sufE"
                     /locus_tag="OO372_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6340404.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO372_000824"
     CDS             complement(44757..45977)
                     /gene="sufS"
                     /locus_tag="OO372_000824"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6340405.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO372_000825"
     CDS             complement(45974..47245)
                     /gene="sufD"
                     /locus_tag="OO372_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6340406.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO372_000826"
     CDS             complement(47220..47966)
                     /gene="sufC"
                     /locus_tag="OO372_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6340407.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO372_000827"
     CDS             complement(47976..49463)
                     /gene="sufB"
                     /locus_tag="OO372_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6340408.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO372_000828"
     CDS             complement(49472..49840)
                     /gene="sufA"
                     /locus_tag="OO372_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6340409.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50388..50576)
                     /locus_tag="OO372_000829"
     CDS             complement(50388..50576)
                     /locus_tag="OO372_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6340410.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO372_000830"
     CDS             complement(50676..51086)
                     /gene="menI"
                     /locus_tag="OO372_000830"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6340411.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51083..54139)
                     /locus_tag="OO372_000831"
     CDS             complement(51083..54139)
                     /locus_tag="OO372_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6340412.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54528..55640
                     /gene="ydiK"
                     /locus_tag="OO372_000832"
     CDS             54528..55640
                     /gene="ydiK"
                     /locus_tag="OO372_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6340413.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13420 a        14656 c        13638 g        14022 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtattggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatt ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta acattgatcg
    40141 ttatcaatta aaattacaaa gcaagaaatt ataagtgaac tgatatttat tatcaattga
    40201 aataaattta acttaagcga caattatttg tgacgttcat tgcagaaaat aaaaataata
    40261 ttatcgttat agattaaaat acataccgca attcgtataa tttcagcagt tgttatttag
    40321 aatgattaac tggaccgcaa ctgaagcaag aatatcagga tgaagcttac cgcctcatcc
    40381 tgaatggtca ttttaccagt gcgttaatga cggtttccat cgcctgcttt tccagctcac
    40441 tgcgctgaat gcgtttattg gctaatgcag tacgtaatac gcctgcgata acaatatgct
    40501 ttggttcctg ccccagatcg cgcatttcca gcacaacttt accgacaact ctgcacattt
    40561 ctcggtacag ttcatcctct tttgtccgat ttcccattct ttatacctat ttatcatgcc
    40621 aactatcagc atatatcaat ctaaccaatt aaacaaaaat caaacaaaat cagacaaata
    40681 acgcgataaa ttattttaac tgtagcaatt gagcgatgat atatttatac accggatgaa
    40741 ctttcactta tcctcacact gacaacttcg gcaccagacg ttgcgcaaac agcgaagttt
    40801 ttgcgtaacc ttttccctgg aacgttaaat ctttgataac aatttattgt ctaacaagtt
    40861 gtatattttt tgaaacgctg tttttgtttt ccttttggat taatttcagc gtataatgcg
    40921 cgccaattga ctcttgaatg gtttcagcac tttggactgt agaactcaac gactcaaaaa
    40981 caggcactca cgttgggctg agacacaagc acacattcct ctgcacgctt tttcgatgtc
    41041 acctatcctt agagcaaggc accaccactt tcgtaatacc ggattcgctt tccggcagtg
    41101 cgcccagaaa gcaagtttct cccatccttc tcaacttaaa gactaagact gtcatgaaaa
    41161 agaccaaaat tgtttgcacc atcggaccga aaaccgaatc tgaagagatg ttagctaaaa
    41221 tgctggacgc tggcatgaac gttatgcgtc tgaacttctc tcatggtgac tatgcagaac
    41281 acggtcagcg cattcagaat ctgcgcaacg tgatgagcaa aactggtaaa accgccgcta
    41341 tcctgcttga taccaaaggt ccggaaatcc gcaccatgaa actggaaggc ggtaacgacg
    41401 tttctctgaa agcgggtcag acctttactt tcaccactga taaatctgtt atcggcaaca
    41461 gcgaaatggt tgcggtaacg tatgaaggtt tcactactga cctgtctgtt ggcaacaccg
    41521 tactggttga cgatggtctg atcggtatgg aagttactgc cattgaaggt aacaaagtta
    41581 tctgtaaagt gctgaacaac ggcgacctgg gcgaaaacaa aggtgtgaac ctgcctggcg
    41641 tttccattgc cctgccagca ctggctgaaa aagacaaaca ggacctgatc tttggttgcg
    41701 aacaaggcgt agactttgtt gctgcttcct ttattcgtaa gcgttctgac gttatcgaaa
    41761 tccgtgagca cctgaaagcg cacggcggcg aaaacatcca catcatctcc aaaatcgaaa
    41821 accaggaagg cctcaacaac ttcgacgaaa tcctcgaagc ctctgacggc atcatggttg
    41881 cgcgtggcga cctgggtgta gaaattccgg tagaagaagt tatcttcgcc cagaagatga
    41941 tgatcgaaaa atgtatccgt gcacgtaaag tcgttatcac tgcgacccag atgctggatt
    42001 ccatgatcaa aaacccacgc ccgactcgcg cagaagccgg tgacgttgca aacgccatcc
    42061 tcgacggtac tgacgcagtg atgctgtctg gtgaatccgc aaaaggtaaa tacccgctgg
    42121 aagcggtttc tatcatggcg accatctgcg aacgtaccga ccgcgtgatg aacagccgtc
    42181 tcgagttcaa caatgacaac cgtaaactgc gcattaccga agcggtatgc cgtggtgccg
    42241 ttgaaactgc tgaaaaactg gatgctccgc tgatcgtggt tgctacccag ggcggtaaat
    42301 ctgctcgcgc agtacgtaaa tacttcccgg atgccaccat actggcactg accaccaacg
    42361 aaaaaacggc tcatcagttg gtactgagca aaggcgttgt gccgcagctg gttaaagaga
    42421 tcacttctac tgatgatttc taccgtctgg gtaaagaact ggctctgcag agcggtctgg
    42481 cacacaaagg tgacgtcgta gttatggttt ctggtgcact ggtaccgagc ggcactacta
    42541 acaccgcatc tgttcacgtc ctgtaatatt gcttttgtga attaatttgt atatcgaagc
    42601 gccctgatgg gcgctttttt atttaatcga taaccagaag caataaaaaa tcaaatcgga
    42661 tttcactata taatctcact ttatctaaga tgaatccgat ggaagcatcc tgttttctct
    42721 cagttttttt atctaaaacc cagcgttcga tgcttctttg agcgaacgat caaaaataag
    42781 tgccttccca tcaaaaaaat attctcaaca taaaaaactt tgtgtaatac ttgtaacgct
    42841 acatggagat taactcaatc tagagggtat taataatgaa agctactaaa ctggtactgg
    42901 gcgcggtaat cctgggttct actctgctgg caggttgctc cagcaacgct aaaatcgatc
    42961 agctgtcttc tgacgttcag actctgaacg ctaaagttga ccagctgagc aacgacgtga
    43021 acgcaatgcg ttccgacgtt caggctgcta aagatgacgc agctcgtgct aaccagcgtc
    43081 tggacaacat ggctactaaa taccgcaagt aatagtacct gtgaagtgaa aaatggcgca
    43141 cattgtgcgc catttttttg tctgctattt accactactg cgtcgcgcgt aacatattcc
    43201 cttgctctgg ttcaccattc tgcgctgact ctactgaagg cgcattgctg gctgcgggag
    43261 ttgctccact gctcaccgaa accggatacc ctgcccgacg atacaacgct ttatcgacta
    43321 acttctgatc tacagcctta ttgtctttaa attgcgtaaa gcctgctggc agtgtgtatg
    43381 gcattgtctg aacgttttgc tgttcttctg ctgatagtgg tcgatgtact tcaacataac
    43441 gcatcccgtt aggttccacg gaatatttca ccggttcgtt gatcactttc accggcgttc
    43501 ccgtccgcac gctggagaac aaggctttaa tatccggtgc attcatgcga atacaccctg
    43561 aactgacgcg caaaccaacg ctgtccggcg cactggtgcc atgaatgagg tattcgccat
    43621 taccatgcgc gaggcgcagt gcgtaacgtc ctagcgggtt atttggtccg gcaggaacga
    43681 ctggcggtaa tttaatgcca cgctccagcg aacgctgacg aatgcctgcc gtaggcgtcc
    43741 aggttgggtt agggattttc tgcccaacac gcgtttccat caccggcgtt tccagcccct
    43801 gcaatccaat acctattgga taaacctgca caatattttc tcccggcgga taataataaa
    43861 ggcgcagctc tgcaaggtta acgataatcc cctgacgcgg tgcatcaggt aataacagtt
    43921 gtgaaggaat agttatcgtc gtaccaggtt ttggcaccgg gacgatagtg ttattggctt
    43981 caaggatcaa cattgccgca gtatcaaaac gtcgggcaat agcctgaagg tttttatccc
    44041 cttcttgcac cgtatacgtt tgattttgcc caaccagtcg gcttccggtt ggtggtagcg
    44101 gataatcaac cgcccaggca gcctggatgg cgctaaaagc gccgataagc gtgagtgtaa
    44161 gcaaagacgc gcgtttcatt gtaaacctcc tgtatttgcc ggagactcac gctgaaacgt
    44221 cggatggcgc ttatgttcac ctgaaaccaa aacactcctg tgcaggtcag tgtaaacatt
    44281 gaccatccgg caatgtgagc caaccggatg aaagctgtcc ctttagttta gctaagtgca
    44341 gcggctttgg cgcgaattgc gcgaatcatc gcttccagac cttgtgaacg agatggggtg
    44401 agatgttggg tgagtgccat tttttcaaac cacggacgca catcgaaact gacaatatcc
    44461 tgcggcgtca tctgatcgta gagaataaag actaccgcaa taagcccttt cacaatcgcc
    44521 gcatcgctgt cgccctgcaa ttcaataatt ccgtgcgcat tctggcgcat gacaatccac
    44581 acctgactct ggcagccctg aatgctattt tgtgggcttc ggtcttcgtc gcgtaattca
    44641 gtcagacgct ggcccagctc aataatgtag agatatttct cttcccagtt ggcgcagcgt
    44701 aaaaaattac gcagcaattt ttctttatcc ggcaataaag ccatagtgcc tccctgttat
    44761 cccagcaaac ggtgaatacg ttgcaggccg gtcaccagac gatccacttc ttcatgggtg
    44821 ttatacatgg ccagcgacgc ccgacacatc gcagggacgt tgtaataggc cattaatggc
    44881 atagcgcagt gatgtccggt acgcacagca atgccgtaat tatcgagaaa actgccaaca
    44941 tcataggcgt ggtgtttacc gagattaaaa gcaataacgc caagcctgtt ttgtgggcca
    45001 tagagagtga gatccggtac agattccagc tgtgatagcg cataatgcat cagattctgt
    45061 tcatactcgg ctatgttatt aagccccagc gccgaaacat actccagcgc cgcgccaaga
    45121 ccaataatgc ccccggtatt gggtgtaccg gcttcaaacc gccatggtgc tttggtccag
    45181 gtagtgcctt cactcaggct gacggtggcg atcatagaac cgcccccttc ccacggcggc
    45241 atctcctgca acaaggcttc tttcacataa agaatgccaa ttccggtggg gccatacagt
    45301 ttatgcccgg agaacacgta aaagtcgcaa tccagcgcct gaacatccac cggatgatgc
    45361 atcaccgcct gagcgccatc caccagcact tttgcgccat gctggtgcgc aagcgtgatt
    45421 atttccgcca gcggattttc tgttcccagc acgttggaga catgagtaat tgccagcagg
    45481 cgagttttct catcaaacag cgtaggcagc gtctccagtt gcagcgtacc gtcggggttg
    45541 agagggatca cacgcagctc tgcgccaacg cgtgcgcaaa gcatctgcca gggaacaatg
    45601 ttagcgtggt gctccatctg actgatgatg atgttatcgc ccgcccgcac gttgctgttg
    45661 ccccaactat tggcgaccag attgatccct tccgtcgtgc cgcggacgaa caccagctct
    45721 tccgccgaac gggcattaat aaacagcgat gcccgcttgc gcacgttctc cattttctcg
    45781 gtcgcctggg cgcttaaggt atgaataccg cgatgcaccg ccgcgtagcc gtgacgataa
    45841 aactcagcct cggcgtcaat cacctggctt ggtttctgcg cactggcggc gctatcgaga
    45901 taagccagcg gcaaaccgtt cacctcacga gaaagcaccg gaaagtcggc ccgcactttg
    45961 tcgacggaaa aagtcatctt gcacctcctg gcagccgttg accgattcgg gccagcacct
    46021 gctgtttaag cccctcatca cgcagtgctt ccgtcagttc ggcagcaaag gcgtaaatga
    46081 tcatctgctg ggcatcctgc tgattgatcc cgcgcgagcg cagatagaac atctgttcat
    46141 catcaatacg ccccaccgtc gcgccgtggc tgcatttcac atcatctgca tagatttcca
    46201 gctgcggttt tgtatccact tccgccattt tgcccatcag cagattattg ttggtcatct
    46261 gaccatccgt tttgatggcg tgctgcgcga cgttgatcaa accgttaaat accgcgcggc
    46321 ctttgtcgct gacgatagtt ttgtgcaact gtcggctgtt acaaaaacct ttattgtgtt
    46381 ccagccaggt acgggtatca cacacctcgt ttttcaccgg catcgccagg ctattgatcc
    46441 gcagcgtgct gttttcgcca ttgagttgcg tactggtgtt gtgtcgtaac actgcgccac
    46501 ccagcaggaa actatggcta aatgcggtgg catcctcagc cagcaacaaa tcgttatgag
    46561 caaagtggtg actgagcggg ttttcaaacg ccagcttgat atgctgcaag tgggcgttcg
    46621 ctgcgacatt gatagtgaac cgtgccccgg taaaatgacg agcatcatta aggctgacaa
    46681 aatgttcgat caccgttgct tcggcacctt ccgccagatc cagatgatgc cggtaatggg
    46741 cagtgttcac ctcttcacct gccacgccct gggtgatatg cattaacagc aatggctttg
    46801 ccggccgttg accgcgcttc acggcgatat gcgtcacgct ttgtgccagg ctttccgtca
    46861 aatgcagaaa cacttccgcc tgaatagcgt cgggtaaccc ctgacggtcg tcgttaatgc
    46921 tcacttcata tccgctgcct tcaattgcat cgctcagcgc gggcacgtaa cgtccatcga
    46981 caaacaccag ccgcacggcg tctaacgtta acgctaaggc atcacgctgc tgtggggata
    47041 tatctcccgc aatgctgaca aactgactat tggtcagccc ttccagcggc gtatatttcc
    47101 agttttcatg tttacgtgtc ggcagtccgg tacgcagcaa ttgttgtaaa tgctgctgtg
    47161 cttgcggtga gcgttttgtc ccttcagctt caaacaagtg atgccactgt tgcagcgcat
    47221 tactgctgtt cggtaagcca gccataaccc tgctcctcca gttgtttgac caacgtgaaa
    47281 tcgccggatt tcacaattcg tccctgatat agcacatgga cgtaatcagg cttgatgtag
    47341 tcgagaatgc gttggtagtg cgtaacaatg atgaatgagc gcttgccgtc acgcagcgag
    47401 ttcacgccat cggcaaccac ttttaatgcg tcaatatcca gcccggagtc cgattcatca
    47461 agaatgcata actccggttc cagcaccgcc atttgcaaaa tatcgttgcg ctttttctcg
    47521 ccgccggaaa aaccaacgtt taccgaacgg gttaataaat cttccggcat cttcaggaga
    47581 gcgattttct cttccatcaa atcctgaaaa tcaaagcggt cgagcgtttc ctggccgcga
    47641 tagctgcgca ccgcattaag tgccgtttgc aggaaaaact ggttactgac gccgggaatc
    47701 tccaccggat actggaaggc cataaagatg ccttcgcctg cacgatcttc cggcgacagc
    47761 gcaagcaaat ctttgccttt gaactcaacc gtgccgcccg tcacttcata atcttctcgc
    47821 ccggcaagcg ttgccgataa ggtacttttg cccgaaccgt ttggccccat aatggcgtga
    47881 acttcgccgg gacgaacgtc gaggcttaat ccacgcagga tagctttatc ttccacgctg
    47941 acgtgtaaat ctttaatact taacatgttt attccttatc cgacgctgtg ttcaagactg
    48001 atggcgagga gtttttgtgc ttcaacggca aattccagcg gcagttctga gaacacgtct
    48061 ttgcagaaac cgttaacaat catcgagatg gcgtcttctt cgctaatccc gcgttgcagg
    48121 cagtaaaaca gttgatcttc accaatacgt gatgtcgttg cctcgtgctc cagttgtgcg
    48181 ctgttattgc gacattcgac atacgggaag gtatgcgccc cacaattagc gccaatcagc
    48241 attgagtcgc actgagtgaa attgcgcgca ttggttgccg tcggcatgat tttcactaag
    48301 ccgcgataac tgttctgact atgtccggca gagatccctt tcgagataat ggtcgatttg
    48361 gtgtttttac cgatgtggat catcttggtg ccggtatccg cttgctgatg accgctggtc
    48421 agcgccactg agtaaaactc accaatggag ttatcgccgc gcaaaatgca gctgggatat
    48481 ttccacgtaa tcgctgaccc ggtttctgat tgcgtccatg acattttgct gttttcgcct
    48541 tcgcacaaag cacgcttggt gacgaaattg agaataccgc cggtgttgtt atcgccaggg
    48601 aaccagtttt gtaccgtgga atatttcacc tcggcgtttt tatggatgat gacttccacc
    48661 accgccgcgt gtaactgata gctgtcacgc accggagcgg aacagccttc aatgtagctg
    48721 acgtagctgt cttcgtcggc caccagaatg gtgcgctcaa actgcccggt tttttccgcg
    48781 ttaatgcgaa aataggtgga aagttccatc gggcagcgca cgcctttagg cacataaata
    48841 aacgtaccgt cagaggctac cgccgcatta agcgcggcaa agaagttgtc atttcccggc
    48901 accacagtgc cgagatattt acgcaccagt tccggatgat cgtggatcgc ctcaccaaag
    48961 gaacagaaaa taattccctg ctccgccagt ttttcgcgat aagtagtggc aaccgaaaca
    49021 gagtcgaaaa tggcatccac cgccacctct ttgccttccc gcacgggaac gcccaactgc
    49081 tcaaacgccg cctccacctc tttacttaaa aaggcgttcg cgccagtttg ctgcaccgcg
    49141 ccaggttcag acgcgcaagt gtcgtcacaa ttaccgcacg atggtgctga gtagtaactg
    49201 taatcctgat aattcagctt gtcgtagtgc gctttcaacc agtgcggttc ttccatctcc
    49261 agccatgcgc gataggcgtt tagacgaaac tccagcatcc actccggctc attacgcttc
    49321 gccgaaattg cgcgcaccac ctcttcgttt atcccctttg ccagctcatc ggtggctaac
    49381 tgggtgaaga atccttcttt ataattcagc gggccgccgg tccaggtttt gacatcgtca
    49441 gttgcttcag tattacgaga catagtaccg cctatacccc aaagctttcg ccacagccac
    49501 attcattctg ggctttaggg ttgtgaaatt tgaatatctg attaagtcct tcacgaacga
    49561 aatcgacttc cgtgccatca ataaacggca tcgcttgcag cgggacaaac agcttcgcgc
    49621 cgtcgtgttc aaacagcaga tcgtctttgt ccggctcgct aacactgtcg agcacatagc
    49681 caaagcccgc gcagcccgtt tgcttcacgc ctaagcgcac gccgaccata cccggctgct
    49741 ttgccaccag ctcacggatg tgtatcgccg ctgcgggtgt cagcgttaag ccttgccagg
    49801 cgaaatcttg tgggttaaag gttcctgaat gcatgtccat cgatttacct cacttcatcg
    49861 ctttcagcgt ataacagcat gctagtgata atgattatca gttcaaccca gcaaacgcag
    49921 gggctttacc gtaaaacatg ctttttgcct gcttttaata agcatagacc ctgatgtgtg
    49981 ggttaacagg cacgctaaga attaggtatc tcattgttag ataatggtta ttatctaagg
    50041 agcattaagg ctgtgatgaa aaagaaaaag ttgtattgaa aatgactatt taagagatag
    50101 gtaaaaaagt gcagcgttca gaaataagaa aacccttaag tctgtgcgac acaggcttaa
    50161 gggtttctac cccatccggc gcttatctcc ggcactctca gtggcttagc tcttgaaggg
    50221 gcgataagaa taatctcata aagctaaccc gccgttttaa cacaaactgc gattagtatt
    50281 atttttgaac aatatcaggc ggtagataag cagtattaag aaggtcatcg aacctggacg
    50341 gaggttaatc caggtcgatt tggcgaactt gcggcattaa gtcaggatca atgcacgccc
    50401 agacgccagg caaagtagat ttcttctttt aattcagtag aagagagagt aagcaggtca
    50461 gcaaattcaa gttctagttg tttcagacgt ttgagatatt gggcaggtga aagattgctc
    50521 tggtcacggc gtaaaaattc aatggccagc tgggtgggat caagttgagt agacatagca
    50581 tcctcgcttt tagacaagac gtgcacagta taccaccgtt tactgtgcag ataatgacca
    50641 aaagcaatat gcgtcacact tttctggtga caacgtcaca aaatggcggt cgtcaatcgt
    50701 gacgaacagc acaaacgccc tttctcatcg aagatttcaa tctgccagac ctggtgacgc
    50761 gaaccgagat gcaacggttt acatatgccg cgcacccgcc cttctcgtgc cgagcggacg
    50821 tgattagcat tgatttccag accaaccact ttttgctcac cttcggtaca taaataaccg
    50881 gcaacggaac cgatactttc ggccagtacc acggatgctc ctccatgcag caacccgaaa
    50941 ggctgctttg tccgcgagtc tactggcatt gtcgcttcaa gggtgtcatc accaatatgt
    51001 tcaaagcgaa tatccaggaa ccccaccatg tttccttcac ccatagcatt cagtgcttcc
    51061 agggtgattt tccgtttcca tatcatttaa taatctccag taaagcctgc acaggatggc
    51121 gtacccccgt gccttcaacc cgttttacct ggctacggca ggaatatccg gtcgccagac
    51181 agcggttacg cggcagtcgc tgcatagcct gatgccagga taactcatag atcccgagcg
    51241 aattttcatg gtttttcgct tcatgtccgt aagtccctgc catgccgcag caacccacgc
    51301 tgacattttc cagtttcgcg ccaaaacggg caaatatcgc ggcccattgt gctggcgcgc
    51361 ccggcaaagc ggtaacctcg gtacagtgac caaagaaata ccatgattca ccgctgactg
    51421 tagccactgg atgtgactca agtgcgcttg ccagccattc attcgcaagt aagacgttaa
    51481 actcgccacg ttcctcgccc agcgccagtt tatattcatc gcgataacaa agtaccagcg
    51541 ccggatcgac gcccaccatt ggcatcccaa gcttagccat acggttgagg aaatccgccg
    51601 tctttttcgc cgtcttcgca aaacgattaa gaaagccttt gatatgctgg gctttgccat
    51661 ttggcgaaaa tggcagtaac acaggctgga aacctaattt ttcaaccaga cggacaaaat
    51721 ccgccaccac ttgcgcatcg taatagctgg taaaggggtc ctgcaccacc aacaccgtgc
    51781 gcgctttctg ctctgcattg agagcttcaa gctgttccag cgtcatgttt gccgagcgat
    51841 gccccaccat ttgttgttgt agcgagggga ccgacagcag tggcaaatca accatgccga
    51901 tatgtttttc cgagagtttg cgcaccagcg gctggttaat gaagaagtta aaggttttcg
    51961 gcgcgcgtgc catcagcggt gcgtagctct cgaccgtagc gacgaggtgg tcgcgcagcg
    52021 ggcgtaaata acgggtgtga tagagctgca ggaaacgaga gcgaaactcc ggcacatcaa
    52081 ttttgatggg gcactgggtc gaacacgctt tacaggccag acagcccgac atcgcctctt
    52141 tgacttcgtg tgagaagtca tattcgcctt tattcgcatg ccagctattg cgcgtgcggg
    52201 caattaacgt ccgtaaactg acgccggatt caggcagttc ttgttccagt ttgagtggat
    52261 caacgccgcg atccgccaac aaacgcagcc attcacgcac cagcgttgcg cgccctttcg
    52321 gtgaatgaat ccggttctgg gtgatcttca tcgacggaca catcggacta cgggcatcaa
    52381 agttgaagca taaaccgttg ccgttacact ccatcgcacc gcgccactgc tggcgtaccg
    52441 caatggggat ctgccgatcg aatgtaccgc gcttcaccgc gtccactttc atcatcggcg
    52501 catcgagacc ttctggcggg caaatcttcc ctgggttgag tcggttatgc gggtcaaatg
    52561 ccgctttcac tttgcgcagt tctgcaaaaa gttcctcacc gaaaaacgcc gggctgtatt
    52621 cagcgcgaaa acctttgccg tgctcgcccc acaacaaacc accgtatttc gcagtcagcg
    52681 ccaccacgtc atcagagatt tgcttcatca aaatctcttg ttgcggatcg cacatatcca
    52741 gtgccggacg gacgtgcaaa acacctgcgt cgacgtgacc gaacataccg tagcttaagc
    52801 cgtggctgtc gagcagcgcg cgaaattcag caatataatc ggccagatgt tccggcggta
    52861 cgcaggtatc ctcagcaaac ggaattggct tagcggcacc tttggcattg ccaagcagac
    52921 caacggcttt tttgcgcatt gcatagatac gttcaacccc cgccagctcg cggcacacct
    52981 gccagccaat cacacctgcc tgttggctgg caatcagctc atcaagccgc acacagagtg
    53041 cattaactcg ctcgtcaatc agcgcctcat catcaccagc aaattccaca atgttcagcc
    53101 cgagcatctc tttgtcaggc acatcggtaa tcaactcgct gacggaatgc cagacaatat
    53161 cttcccgcgc cagattcagc acttttgagt ccaccgtctc taccgaaagc gcccgcgcct
    53221 caaccataaa cggcgcgtta cgcagcgcgg agtcaaaaga gtcatatttg acgttcacca
    53281 gacggcgcac tttaggcaag ggcgtaatat ccagccgcgc ttcggtaata aaggccagcg
    53341 tcccttctga acccgtcaga atgcgcgtca ggtcgaactc ggtcatctca tcgttaaaga
    53401 catgacgcag atcgtaaccg gtaagaaagc ggttaagttt ggggaagttg tcgatgatta
    53461 actggcgttg cagacggcaa cgttgataaa ccgtgttata aattcgcccg attgtggtat
    53521 tggttttacc cagcgtttcc gccagttcga cgggtaaagg ttgcgtatcg agaatatcgc
    53581 cccccaacaa caccgcgcgt acgccaagta cgtgatctga cgttttgcca tagaccagcg
    53641 atccctgacc ggatgcatcg gtattgatca tcccgccgag cgttgcccgg ttgctggtcg
    53701 aaagttctgg tgcaaaaaag tagccgaacg gtttcaggta ctgattgagt tgatctttta
    53761 tcaccccggc ctcaacgcgc acccagccct cttcagggtt aatttcgatg atgcggttca
    53821 tatggcggga catatcaaca ataatcccct ggttgagcgc ctgaccgtta gtgccggtgc
    53881 cgccgccgcg gggggtaaag atcagcgatg aatagcgttc ctgcgcggca agacgggcga
    53941 tcagcgccac atctgcggtt gaacgcggaa ataccaccgc atcggggaga agttggtaaa
    54001 tactgttgtc ggtcgacatt gtcagacgat cggcataact tgtcgccgta tcgccggtaa
    54061 aaccttgttg ctccagctct tgcaaaaaat taagcaccag ttgaacgacg ccgggtgcct
    54121 gggaaatctg tggaatcatt atattgaccc tttcctgcgg tctgtgatgt aggtcgatac
    54181 actattcttt caggcggctg caatagcgca ctgaaaggtg atgtttattt actctatgga
    54241 tttcgagttg caggaaggcg gcaagcgagt gaactccagg agcttacaat agtaagtgac
    54301 tggggtgaac gaacgtagcc gcagcgcatg caacttgaaa tacgacgagt aaatcgtttg
    54361 cgtgttgcct gagttgttgt accacatttt tttctaacac gcccatcaga attaagggca
    54421 gaatcggcct gttaaaaacc gctgaaattg ctcatcatta tgcaggtgag tttcgcgtgt
    54481 tcacgtcgcg tcgacgattt gacgcacaaa aaaggtgaaa agtagttatg gtaaatgttc
    54541 gtcagcccag ggatgtcgca caaattctgc tttcggtgct gtttttagcc atcatgattg
    54601 tggcatgtct gtggattgtt caacccttta ttctcggctt tgcatgggcc ggtacggtgg
    54661 ttatcgccac ctggccggta ttgttacgtt tgcaaaagat catgtttggc cgccgctctc
    54721 tcgccgttct ggtgatgacg ctgttattag tgatggtgtt tatcatccct attgctttgc
    54781 tggttaacag tatcgtcgac ggcagcggcc cgctaattaa agccatttcc agcggcgaca
    54841 tgacgttacc cgatctggcg tggcttaata ccattccggt gattggcgcg aagctgtatg
    54901 caggctggca caacttgctg gatatggggg gtacggcgat catggcgaaa gtccgccctt
    54961 atattggcac caccaccacc tggttcgttg ggcaggcggc gcatatcggg cgctttatgg
    55021 tgcattgtgc gctgatgctt ctcttcagtg ccctgctgta ctggcgcggt gaacaggtgg
    55081 cacaaggcat tcgccatttt gcaacccgtc tggcaggcgt tcgcggagat gccgccgtgc
    55141 tgctggcggc acaggctatc cgcgcggtgg cgctgggtgt ggtggtgacg gcgttagtac
    55201 aggcagtgct tggcagtatc ggcctcgccg tatccggcgt accttatgca acgttgctaa
    55261 cggtgttaat gatcctctcc tgccttgtcc agcttggccc gttgccggta ctgattccgg
    55321 cgattatctg gctctactgg accggcgata ccacctgggg aacggtattg ttagtgtgga
    55381 gcggtgtggt tggcacgctg gataacgtca tccgcccaat gttaattcgc atgggtgccg
    55441 atttaccgct gatcctgatc ctctctggcg ttattggtgg tttgattgct ttcgggatga
    55501 tcggtctgtt tattggtccg gttctgttag ccgtttcctg gcgtctgttt gccgcgtggg
    55561 tggaagaagt cccgccgccg acggaccaac cggaagaaat tctcgaagaa cttggcgaaa
    55621 tcgagaaacc gaataagtaa tttctcatca ggcggctctg ccgcctgatt gttaatcacc
    55681 gctaattgat atttgcttgc tcttccccat attttcctgc ttaccgccat tcagct
//
LOCUS       DAJTVK010000013        53402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000013 DAJTVK010000000
VERSION     DAJTVK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.013"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="OO372_000833"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="OO372_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6340414.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="OO372_000834"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="OO372_000834"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6340415.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="OO372_000835"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="OO372_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6340416.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3697..5964
                     /gene="adiA"
                     /locus_tag="OO372_000836"
     CDS             3697..5964
                     /gene="adiA"
                     /locus_tag="OO372_000836"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6340417.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGASGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6289..7050
                     /gene="adiY"
                     /locus_tag="OO372_000837"
     CDS             6289..7050
                     /gene="adiY"
                     /locus_tag="OO372_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6340418.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7187..8524
                     /gene="adiC"
                     /locus_tag="OO372_000838"
     CDS             7187..8524
                     /gene="adiC"
                     /locus_tag="OO372_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6340419.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8628..10271
                     /gene="eptA"
                     /locus_tag="OO372_000839"
     CDS             8628..10271
                     /gene="eptA"
                     /locus_tag="OO372_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6340420.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10268..10936
                     /gene="basR"
                     /locus_tag="OO372_000840"
     CDS             10268..10936
                     /gene="basR"
                     /locus_tag="OO372_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6340421.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10937..12037
                     /gene="pmrB"
                     /locus_tag="OO372_000841"
     CDS             10937..12037
                     /gene="pmrB"
                     /locus_tag="OO372_000841"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6340422.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OO372_000842"
     CDS             complement(12013..12102)
                     /gene="pmrR"
                     /locus_tag="OO372_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6340423.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OO372_000843"
     CDS             complement(12214..13716)
                     /gene="proP"
                     /locus_tag="OO372_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6340424.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13980..14846)
                     /locus_tag="OO372_000844"
     CDS             complement(13980..14846)
                     /locus_tag="OO372_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6340425.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OO372_000845"
     CDS             complement(14843..17071)
                     /gene="crfC"
                     /locus_tag="OO372_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6340426.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17484..17819
                     /locus_tag="OO372_000846"
     CDS             17484..17819
                     /locus_tag="OO372_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6340427.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17977..18420
                     /gene="yjdN"
                     /locus_tag="OO372_000847"
                     /pseudo
     CDS             17977..18420
                     /gene="yjdN"
                     /locus_tag="OO372_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18553..19341
                     /gene="phnC"
                     /locus_tag="OO372_000848"
     CDS             18553..19341
                     /gene="phnC"
                     /locus_tag="OO372_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6340428.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19366..20382
                     /gene="phnD"
                     /locus_tag="OO372_000849"
     CDS             19366..20382
                     /gene="phnD"
                     /locus_tag="OO372_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6340429.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20508..21287
                     /gene="phnE"
                     /locus_tag="OO372_000850"
     CDS             20508..21287
                     /gene="phnE"
                     /locus_tag="OO372_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR6340430.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21308..22033
                     /gene="phnF"
                     /locus_tag="OO372_000851"
     CDS             21308..22033
                     /gene="phnF"
                     /locus_tag="OO372_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR6340431.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22034..22486
                     /gene="phnG"
                     /locus_tag="OO372_000852"
     CDS             22034..22486
                     /gene="phnG"
                     /locus_tag="OO372_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR6340432.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22483..23067
                     /gene="phnH"
                     /locus_tag="OO372_000853"
     CDS             22483..23067
                     /gene="phnH"
                     /locus_tag="OO372_000853"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR6340433.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23067..24131
                     /gene="phnI"
                     /locus_tag="OO372_000854"
     CDS             23067..24131
                     /gene="phnI"
                     /locus_tag="OO372_000854"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR6340434.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24124..24969
                     /gene="phnJ"
                     /locus_tag="OO372_000855"
     CDS             24124..24969
                     /gene="phnJ"
                     /locus_tag="OO372_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR6340435.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24966..25724
                     /gene="phnK"
                     /locus_tag="OO372_000856"
     CDS             24966..25724
                     /gene="phnK"
                     /locus_tag="OO372_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR6340436.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25835..26515
                     /gene="phnL"
                     /locus_tag="OO372_000857"
     CDS             25835..26515
                     /gene="phnL"
                     /locus_tag="OO372_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR6340437.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26512..27648
                     /gene="phnM"
                     /locus_tag="OO372_000858"
     CDS             26512..27648
                     /gene="phnM"
                     /locus_tag="OO372_000858"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR6340438.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27648..28205
                     /gene="phnN"
                     /locus_tag="OO372_000859"
     CDS             27648..28205
                     /gene="phnN"
                     /locus_tag="OO372_000859"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR6340439.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28192..28626
                     /gene="phnO"
                     /locus_tag="OO372_000860"
     CDS             28192..28626
                     /gene="phnO"
                     /locus_tag="OO372_000860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR6340440.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28628..29386
                     /gene="phnP"
                     /locus_tag="OO372_000861"
     CDS             28628..29386
                     /gene="phnP"
                     /locus_tag="OO372_000861"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR6340441.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29533..29862)
                     /gene="yjdP"
                     /locus_tag="OO372_000862"
     CDS             complement(29533..29862)
                     /gene="yjdP"
                     /locus_tag="OO372_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR6340442.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29931..30248)
                     /locus_tag="OO372_000863"
     CDS             complement(29931..30248)
                     /locus_tag="OO372_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR6340443.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30501..32486
                     /gene="yjcS"
                     /locus_tag="OO372_000864"
     CDS             30501..32486
                     /gene="yjcS"
                     /locus_tag="OO372_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR6340444.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32695..32970
                     /locus_tag="OO372_000865"
     CDS             32695..32970
                     /locus_tag="OO372_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340445.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            32989..34020
                     /gene="mdtN"
                     /locus_tag="OO372_000866"
     CDS             32989..34020
                     /gene="mdtN"
                     /locus_tag="OO372_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6340446.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            34020..36071
                     /gene="mdtO"
                     /locus_tag="OO372_000867"
     CDS             34020..36071
                     /gene="mdtO"
                     /locus_tag="OO372_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6340447.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            36068..37534
                     /gene="mdtP"
                     /locus_tag="OO372_000868"
     CDS             36068..37534
                     /gene="mdtP"
                     /locus_tag="OO372_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6340448.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            37732..39879
                     /gene="fdhF"
                     /locus_tag="OO372_000869"
     CDS             37732..39879
                     /gene="fdhF"
                     /locus_tag="OO372_000869"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:38149..38151,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6340449.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            39973..40662
                     /locus_tag="OO372_000870"
     CDS             39973..40662
                     /locus_tag="OO372_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6340450.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(40741..42054)
                     /gene="gltP"
                     /locus_tag="OO372_000871"
     CDS             complement(40741..42054)
                     /gene="gltP"
                     /locus_tag="OO372_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6340451.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(42396..42992)
                     /gene="nrfG"
                     /locus_tag="OO372_000872"
     CDS             complement(42396..42992)
                     /gene="nrfG"
                     /locus_tag="OO372_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6340452.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(42989..43372)
                     /gene="nrfF"
                     /locus_tag="OO372_000873"
     CDS             complement(42989..43372)
                     /gene="nrfF"
                     /locus_tag="OO372_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6340453.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(43365..45083)
                     /locus_tag="OO372_000874"
     CDS             complement(43365..45083)
                     /locus_tag="OO372_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6340454.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(45103..46059)
                     /gene="nrfD"
                     /locus_tag="OO372_000875"
     CDS             complement(45103..46059)
                     /gene="nrfD"
                     /locus_tag="OO372_000875"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6340455.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(46056..46727)
                     /gene="nrfC"
                     /locus_tag="OO372_000876"
     CDS             complement(46056..46727)
                     /gene="nrfC"
                     /locus_tag="OO372_000876"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6340456.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(46724..47290)
                     /gene="nrfB"
                     /locus_tag="OO372_000877"
     CDS             complement(46724..47290)
                     /gene="nrfB"
                     /locus_tag="OO372_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6340457.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(47335..48771)
                     /gene="nrfA"
                     /locus_tag="OO372_000878"
     CDS             complement(47335..48771)
                     /gene="nrfA"
                     /locus_tag="OO372_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6340458.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            49165..51123
                     /gene="acs"
                     /locus_tag="OO372_000879"
     CDS             49165..51123
                     /gene="acs"
                     /locus_tag="OO372_000879"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCR6340459.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            51323..51636
                     /locus_tag="OO372_000880"
                     /pseudo
     CDS             51323..51636
                     /locus_tag="OO372_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            51633..53282
                     /gene="actP"
                     /locus_tag="OO372_000881"
     CDS             51633..53282
                     /gene="actP"
                     /locus_tag="OO372_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6340460.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT        12828 a        14707 c        13893 g        11974 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataat ttaaaataat cacataacgc attgtttata aaaataaata
     3601 tttcaaaaat gtttgttttt cacgcgcttt acagcccgaa aaggccggaa gatacttgcc
     3661 cgcaacgaag attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc
     3721 gagtttctcc atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta
     3781 agccagcaaa atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc
     3841 tcttcaaacg aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa
     3901 catcaaaacg tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc
     3961 ttcctgttgg gcgatcggga aaaagccctc gccgcaatgg atcgcgacct gctggagctt
     4021 gtcgatgaat tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgctgtt
     4081 gccgcgatga cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa
     4141 tatagcgaca tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt
     4201 accaaaacac ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc
     4261 gatatgggca tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc
     4321 gaaagcgaaa aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc
     4381 ggtgcttccg gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg
     4441 gtcgttgacc gtaactgcca taaatccatc gaacaaggct tgatgctgac aggcgcgaaa
     4501 ccggtctaca tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag
     4561 gaaatgcaac ctgaaacctt gcagaagaaa atcagtgaaa gcccgctgac taaagacaaa
     4621 gccgggcaaa aaccgtctta ctgcgtggtg accaactgca cctatgacgg cgtgtgttat
     4681 aacgctaaag aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa
     4741 gcctggtacg gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc
     4801 gaacctggcg atcacaacgg tcctaccgtt ttcgccaccc actccaccca caaactgctg
     4861 aatgcgcttt cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc
     4921 tcccgcttca accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc
     4981 gcatccaacg acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacgcag
     5041 gaagtgatcg acgaagcggt tgatttccgt caggcgatgg cgcggctgta taaagagttc
     5101 accgctgacg gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa
     5161 accggcaaaa cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac
     5221 tgctgggtaa tgcatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg
     5281 agtatgctcg acccgattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa
     5341 ctggaagaaa ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt
     5401 gtgcctaccc gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt
     5461 gggaaatggg gaactctggt taacaccctt tgctccttca aacgccacta tgacgccaac
     5521 acaccgctgg cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac
     5581 atggggattc acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc
     5641 gcacggttga acgaagccta ttccggcctg tcggtggcgg aaatcacccc gcgtgaagcg
     5701 tacaacgcga ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc
     5761 atcgcggcaa actcagttat cccatatccg ccaggaatcc cgatgctgct gtctggtgaa
     5821 aacttcggcg ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac
     5881 caccatttcc ctggatttga acacgaaact gaagggactg aaattattga cggtatttac
     5941 cacgttatgt gtgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac
     6001 attacgccgc ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca
     6061 aacaaaaatg tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta
     6121 tcctgtaaaa aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact
     6181 cgttattttt atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc
     6241 tttgctttag cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc
     6301 agcgaccaac cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg
     6361 aaagaaccta ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt
     6421 atcgacgtct cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa
     6481 gattacctcc ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct
     6541 acgcccatac tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac
     6601 agcatgatgc ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg
     6661 gtgctatccc gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt
     6721 aattgtgtaa gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat
     6781 cttagtatgg tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagctgaaa
     6841 agcgaaaaca ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat
     6901 gaattaatga tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt
     6961 acgtcgtact ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt
     7021 agtcagcaca gagaacgaac tgtcgcctga tttttaacct taacgaagag ctatattaat
     7081 aacggcatca gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata
     7141 tgacgtaatt tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc
     7201 tgatgctcac aaagtgggct taatccccgt caccctgatg gtgtcgggga atattatggg
     7261 gtcaggtgtt tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg
     7321 atggttggtg acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt
     7381 cctcgatcca agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct
     7441 cggttatcaa accaacgtcc tctactggct ggcctgctgg atcggcaata tcgctatggt
     7501 ggtcattggc gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt
     7561 aaccatcacc tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa
     7621 aatgatcacc cgtgtgcagg ccgttgccac cgtgctggcg ctgatcccca tcgtcgggat
     7681 tgccgtattt ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag
     7741 cggcctgggc accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat
     7801 cggtgtggaa agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc
     7861 tatcgccacc attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc
     7921 gattatgggg atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc
     7981 cgcacggatg gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg
     8041 ttgcttaggt tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc
     8101 cgatgacgga ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc
     8161 ggggctgatt atcgtcggta ttttgatgac catcttccag ctcagtagca tttcgccaaa
     8221 cgcaaccaaa gagttcggtc tggtatcttc cgtgtcggtc atctttacac tggtgccata
     8281 tctttacacc tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc
     8341 ggcatatctg gctgttacta ccattgcctt cctctactgc atctgggccg tggtggggtc
     8401 cggggcgaaa gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc
     8461 gctgaattac aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga
     8521 ttaattcccc ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa
     8581 ttttgctttg cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc
     8641 tactaaaaag cccctctttg aatttactcg cctggctatt gttggccgct ttttatatct
     8701 ctatctgcct gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc
     8761 tgcataacgt actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg
     8821 tcctgacact aagctctttc ttatggctta atcgaccact ggcctgcctg tttattctgg
     8881 ttggcgcggc tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga
     8941 ttgccaatat tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt
     9001 tattaacgct gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca
     9061 aacctgccac ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg
     9121 tactgctgat tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca
     9181 ataacaaaga gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat
     9241 ggtactccca tcagcgactg gcaaatctac cgctggtgcg aattggtgaa gacgcccacc
     9301 gcaacccgtt aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa
     9361 cctcgcgggc ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg
     9421 cgaaagataa cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt
     9481 cagtaccgtg catgttctcg gatatgccgc gtgagcatta caaagaagag ctggcacagc
     9541 accaggaagg cgtgctggat atcattcagc aagcgggcat caacgtgctg tggaatgaca
     9601 acgatggcgg ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga
     9661 atctgcctgg tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg
     9721 aagagtacat caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc
     9781 acggtccgac ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg
     9841 acaccaatga gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc
     9901 tggtttacgt cgactatatt gttgataaag cgattaatct gctgaaagaa catcaggata
     9961 aatttaccac cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca
    10021 tctatctgca cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccgatgc
    10081 tgctgtggct gtcggaggat tatcaaaaac ggtatcaggt tgaccagaac tgcctgcaaa
    10141 aacaggcgca aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa
    10201 ctggcgttga gacgaagtat taccaggctg cggatgatgt tctgcaaact tgcaggagag
    10261 tgagtgaatg aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct
    10321 ggcggcgcaa accgaaggct acacgtgcga tggcgtaaca accgcgcgga tggcggaaca
    10381 aagccttgag gccggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga
    10441 tggactgcat tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct
    10501 caccgctcgc gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta
    10561 tctggtgaag ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg
    10621 ccataataat cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg
    10681 ccgtcaggta tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct
    10741 gtcacggtta atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat
    10801 ctataactgg gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg
    10861 cgacaaagtg ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc
    10921 gaatgaggaa aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca
    10981 acggctgata ttgaccatcg gggccatttt gttggtgttt gagctgatca gcatcttctg
    11041 gctatggcat gaaagtaccg agcagattca gctgtttgag caggcgctac gcgacaatcg
    11101 caacaacgat cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc
    11161 cggcgtcttt atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac
    11221 ccgcccgctg gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc
    11281 cattgccatt cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct
    11341 ggtcagtcgc ctgacgagca cgctggataa cgaaaggtta tttaccgctg acgtcgcgca
    11401 cgaactgcga acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca
    11461 tcacattgat gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca
    11521 gctgctgcaa ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa
    11581 actgctggaa gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg
    11641 acagcaaacc ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac
    11701 cctgctgcgg atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccacaagg
    11761 cagcaacatt atgattaagc tgcaagaaga tggcggagcg gtcatggccg ttgaagatga
    11821 aggaccgggt attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga
    11881 cagccgttat ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca
    11941 tcacgggcag tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg
    12001 gctgaagaaa gatcagtacg tagcaaacca gatataaaga aagctgctga ccaccagcac
    12061 actgaacacg gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa
    12121 cctggaggag agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat
    12181 aagacagcgt cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg
    12241 caccaggcgg gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat catcgatttt
    12301 ctgctcgata ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc
    12361 cgccggtgtc gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat
    12421 taaaccaatc accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct
    12481 ttcgaccagc caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa
    12541 tgccgccgcc agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc
    12601 cataacgccc gtaaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag
    12661 gccgatgacg ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagggcaac
    12721 actaccaagt agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg
    12781 ctggacaaac agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga
    12841 gtaatgcagg ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac
    12901 gttggtggca attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat
    12961 ctctttaaac gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt
    13021 atcaacatgc tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat
    13081 aatccctaac ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc
    13141 ttcgccgaca atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat
    13201 agaaccgaag tccagccagc tgcccataaa gccacgttta cggtcagggg agtattccgc
    13261 aacaaatatc gacgccccgg tatattcacc gccgaccgag aaaccttgtg ccatcttaca
    13321 gatcaacagc agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat
    13381 acagaacgta ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata
    13441 tttatcgccc aacataccaa agaagagccc gccaagcggt cgaatcagaa agggaacgga
    13501 gaaagtggca agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt
    13561 acctaatgcg taagcaacaa aaccataaac accaaaatcg aaccattcca ttgcattacc
    13621 cagtgatgct gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg
    13681 aagggtaatc ggttttactt ttttcctttt cagcatagct ttcctcgcag agttggccca
    13741 tttccgcaaa cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg
    13801 atgcaatctc tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca
    13861 atcaaaccag ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta
    13921 aatttgtgat caacttcaca catggatttc gtttaggact cattgatgta actgattatt
    13981 tacgccaaat ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa
    14041 cagagtcgtg gcatccccag cgcgactgtt tgccagcgtt gggtcactga ccagcaaacg
    14101 ggctgtttat ctgccgtgga ccactttccc agccaactta aaaaatcagc atgctcccct
    14161 cccgaagagg caaaccactg atggtaatag tgacgcgtgg tcggtgccgc attgatactc
    14221 tctgccagat gttgtgtcgc tttctcgcgc aaatcaccca gtaacaacac caccgcttgc
    14281 gggcttccca gacggcaggc atcgccaaat gcaccccagc gcaactcctc cagaacgata
    14341 aaacagcgcc caggcaacga ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt
    14401 tgctcgctct gatcacctgc ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc
    14461 cagcgacaac gggctgaaaa ccactcatac aaggcggcaa tttgctgggg gagctccggg
    14521 aaagcagagt gctggcggtg atccattttt tgcagcagcg ccagatgctg cgcgatctgc
    14581 gtcagcgcca gatgaccggg atccagcatc gacgccagtt tttccaccat tgtcagtcgc
    14641 aggctaagcg gcgcatttaa catgccgccg ttcgtccatg tctgaagctg cacacgcgaa
    14701 agaagttcct gcatcagtcg ttcacgaaac tgctgctgat gggcttgtgg aaggcgggca
    14761 tcatcgccct gcgccagatc cactaaaaag cggggataaa ccgactccag cacgcgaccg
    14821 gggccgtcca gtaacgtctt ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat
    14881 cgcgtaacag ctgggcgtcg gtgtaaatcc agccgtgagt catcacagtc tgctgcaatt
    14941 gctgctgcat cagcctgacc accgattcat tttgttgacg cagagccagg ctttcgcgta
    15001 aacgcgtctg taattccgtc aaacatgaag cgaactcagc gcaaaaagta ttcatgcctg
    15061 ccgtaacaga ttcatcgacc tgctctgcca gaactttagc catttgttgg caataaagat
    15121 cgacttctgc gcttaaagct cgttgcaaca cactgtaatc aaccgtctct gtcggggatt
    15181 tctcatttcc ccgtccccag tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa
    15241 cgcctaaacg cgtgctctgc tcgtccgttg catcctggcg cgaaatgact tcactgaaca
    15301 gctggcgagt gttgacgtgg ggaactactc cgtgaaaaac aggaaaatga accccaggac
    15361 gaaaccctga ctcgctcaat tccattttga cttgttgctc aatgggccga ataacatcgg
    15421 ttaacacccg gcaaagggtg gattcaagct cggcaaaacg cagcgtaaag tcgcgactga
    15481 tggtgttctg cgccgtctgt aacagtgtct cacagcaggt acgaatctcg cttaacggct
    15541 ccgaatcatc ctgaaacaag gcggctaact gcgcattcag cgcatcttgc tgttgacgca
    15601 gaaagtggtt ggcggaggtc agggccagct cgatttcatg tttaatctcg ccgctcacct
    15661 gcgcctgatt gagttgcaat agctgcaaac tttcttcgac ctgatggata ttttgccgca
    15721 attgttcaca agcgacgttt aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct
    15781 gcgcgtaatt caacagttta tgcgcagcag atcgcagagc atacaacgag gcgttagcgt
    15841 aagcggcatg aagcaacgcc tgaattggct gggcgaacag cgaatcttcc cacaactgat
    15901 cggcagcatg acgaatatgt tcgaggtccg ccagatcggc atgacgccag cgcctgccga
    15961 gcgcggcatg ggcaaaatct tccacccagc gttgttgctc tggcgctggt aacttaccgt
    16021 tgttggctaa ctcatggcgc gctcgatttg ccaagtagcc ccacatcgac gacaccggaa
    16081 atatctgctg tggcgtaata cagcctttca tcagcgtccc ggaaatcagt gcccgcacct
    16141 ggtcggcgtc gtcactgtta cgatcctgtt gatcgaactt attgaccagc acatacagtg
    16201 gcaccgattg ccccaccgcc aaaatcgcct cacggacctc ttcatcggag atcgatttca
    16261 gttgcgtata atccagcacc gccagtaccg ccgaggcgcg tgccagctgc tggttaagca
    16321 ttttttgcag atgcggttgc ccggcttcat ttggcccggg ggtatccagt aacgtcaact
    16381 gaccgggata actctccagc cccgccagat ggacaaactc cacttcaatc acgggaatat
    16441 gctcaatggc ggcgtaagca gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca
    16501 ctaaatcatt caaacttttc agacaatgaa aaataggctg ggcacccaga taatattttt
    16561 cgaaagcgac gccattttcg atccgctgca taagcgcacg catatcttta tctatttcca
    16621 gcacatcggt cagatgctta atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta
    16681 aacaatcgat tggcgcgaca tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat
    16741 ggcgaataag cgtcggcagc gcagtcattg ggcgattacg attaggcaga acctccgtac
    16801 caacaatggc attaatggtg gttgatttcc ctgctttcat agtaccgaca atcgccagca
    16861 ccatttccag tcgggaaatt ttactcaact cattattcag catcgcgtga cgttcggcga
    16921 tattaggctg actccagggt aaagccagtt gtggcgcgtc gtctccgggt acagagagag
    16981 gcattttttc cagtaactgc aactgttggc gagaaagctg taacaggcgt tcagcctcct
    17041 gacttaactc atacagggtc tgtgtgtaca tagaaaattc ttccttaaag caaattttgt
    17101 tacttttatt tagccagatt gtttttgagt tctgtttgag ttctgttttt ggcttttata
    17161 attactgcaa gaaataattt tatatttagt gtgttgtttt ttatcagaat aaataacgtc
    17221 ttctgatgcg tttaaaacgt cagaaagata aaaatatcat gtgaataaaa aaaagaacaa
    17281 gtagagcatt aacattatct taaataataa atagaggcaa aaagattatt ttctttttgc
    17341 gtttcctttc aaatgaaaac gatcgtcgtc taaaatcagc agtacccccg acaaactcag
    17401 ggattttgtg tataattgcg gcctttttcg gcaatctgcc gttttttggc gcttttgccc
    17461 tgctgacttt tgaggaaatc cacatgtcat taccacactg cccaaaatgc aactccgaat
    17521 acacttacga agataacggc atgtacatct gcccggaatg tgcctacgaa tggaacgacg
    17581 cagaacctgc acaggaaagc gacgagctga tcgttaaaga tgctaacggc aatctgctgg
    17641 ctgacggcga cagcgttacc atcattaaag atctgaaggt gaaaggtagc tcttcgatgc
    17701 tgaaaattgg caccaaagtg aaaaacatcc gcctggttga aggcgaccat aacatcgatt
    17761 gcaaaatcga cggttttggt ccgatgaaac tgaaatctga gtttgtgaaa aagaactgat
    17821 tgtattgtga tcggtaagcc ggataaggcg ctcgcgccgc atccggcaac gatgccgact
    17881 gcctgatgcg acgcttgcgc gtcttatcag gcagcctctc atctgtataa atttcgaact
    17941 acacttaatt ggcttctctt aactgaggtc accatcatgc cgttaagtcc ctacctctct
    18001 tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg cgctgaactg
    18061 ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc cgagaactgc
    18121 ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaatgtgcg cattgccgga
    18181 agcgacatca tgatgagcga tgccatgccg tcaggaaaag ccagctactc cggctttacg
    18241 ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa tcttgccgct
    18301 aacggaaaaa tcgaaatggc ctggcaggaa actttctagg cgcatggctt tggcaaagtc
    18361 accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc aacgcaataa
    18421 cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc ttcatctgtt
    18481 agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc acttttttag
    18541 ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac cttcaatcag
    18601 catcaggcgc tgcatgcggt tgctctgaac attcatcacg gtgaaatggt ggctctgctt
    18661 gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt gattaccggc
    18721 gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg cgaaggccgt
    18781 ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca acaattcaac
    18841 ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg cagcacgccg
    18901 ttctggcgca cctgttttag ctggtttacc cgcgagcaga aacaacgcgc gttacaggcg
    18961 ctgacccgcg ttggcatggt gcattttgcc catcaacgcg tttccaccct ctccggcgga
    19021 cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt gattctggcc
    19081 gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga caccctgcgc
    19141 gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt ggattacgcc
    19201 ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta cgacggcagc
    19261 agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa tcgcatcgaa
    19321 gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa cgctaagata
    19381 attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag cccggcacac
    19441 gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc acagcaaaac
    19501 ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg cgtgaaggtg
    19561 aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt caataaagtg
    19621 gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc caacggccag
    19681 gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt gttgatcgtc
    19741 aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa agatctcacc
    19801 ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta ctacgtcttt
    19861 gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgccgg gcatgaaacc
    19921 aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac cgaaaacctc
    19981 gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat ctggaagtcg
    20041 ccgctgatcc caggcgatcc gatcgtctgg cgcaagaatc tctccgaaac caccaaagac
    20101 aagatctacg acttctttat gaactacgga aaaacgccgg aagagaaagc ggtgctggaa
    20161 cgcctgggct gggcaccgtt tcgcgcctcc agcgacctgc aactggtgcc gattcgccag
    20221 ctcgcactgt ttaaagagat tcagggcgta aaaagcaata acggactgaa tgagcaggac
    20281 aagctggcga aaaccaccga gattcaggcg cagctggatg acctggaccg cctgaacaac
    20341 gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aatgtgctcg atcggtcccc
    20401 tctcccttga gggagagggt tagggtgagg gggaaatgcg cacgctgatg ccctcacccc
    20461 ggccctctcc cacagggaga gggagaaaac attaaacgga gccaaacatg caaaccatca
    20521 ccatcgcccc acccaagcgc agctggttct cgcttctgag ctgggccatt gttctcgccg
    20581 tgttggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatc aaagacggcg
    20641 gcaacatggc gaccttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt
    20701 acctcaccga aatggccgtc acgctgcaaa tcgccgtctg gggcaccgcg ctggcagtgg
    20761 ttctctccat ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc
    20821 agcccgttcg ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc
    20881 tgttcgtggt cgccgtcggt ctcggaccgt tcgctggcgt gctggcgcta tttattcaca
    20941 ccaccggcgt gctctcgaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg
    21001 tggaaggcat tcgcgccacc ggcgccaaca agctggaaga gatcctttac ggcgtgctgc
    21061 cacaggtaat gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct
    21121 cggcgaccgt cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga
    21181 ttcgcggttt ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca
    21241 gcctgctgga tttcctctct caacggttgc gtaagcactt tatctgataa gtgaggcatt
    21301 gatatctatg cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc
    21361 cgcaaaactt gagcaggagc ttcgtcaaca ctatcgctgc ggcgactatc ttcccgccga
    21421 gcagcaactg gcggcgcgct ttgaggtaaa ccgccacacc ctgcgccgcg ccatcgacca
    21481 actggtggaa aaaggctggg tacagcgccg tcagggcatc ggcgtgctgg tgctgatgcg
    21541 cccgttcgat tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg
    21601 cagccatccc accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgttgc
    21661 tgacgcgctg gggattaccg agggtgagaa cgttatccat ttgcgcaccc tgcgtcgcgt
    21721 caacggcgtc gcgctctgtt taatcgacca ctactttgcg gacctcaccc tctggccgac
    21781 actgcaacgt ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc
    21841 gctgcgccgc agccagacgc ggatcagcgc ccgccgcgcc caggccaaag agtgccagcg
    21901 ccttgaaatc ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg
    21961 tgaaagcagc ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac
    22021 tatggagcac tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc
    22081 gcacagccaa ccggctgaac tggcagcccg cctgaacgcg ctgaacatca ccgccgacta
    22141 tgaagtgatc cgcactgccg aaactggcct ggtgcagatt caggcgcgaa tgggcggtac
    22201 cggcgaacgc ttttttgccg gcgacgccac gctgacccgt gccgccgtac gcctgactga
    22261 cggcaccctc ggctacagct gggtgctggg gcgtgataaa cagcacgccg aacgctgcgc
    22321 gctgattgat gcgctgatgc agcaatctcg ccactttcaa aacttatcag aaacccttat
    22381 tgccccgctg gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc
    22441 cagccgggtc gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct
    22501 tttatgcttc ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc
    22561 gagccgggcg tgattgtcgc cctgtatcag ctcaaacgcg gctggcagcc gctgaatatc
    22621 gccaccacca gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccacg
    22681 ccattaagta acgatatcgt caatcagagc ctgcgttttc ataccaacgc gccgctggtc
    22741 agccagccgg aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctc
    22801 aacgcccttt ccaccgacac cgccgttgcg ccggaagcgg gcgcgacgct gattttacag
    22861 gtcgccagcc tgagcggcgg acgcatgttg cgtctcaccg gcgcgggcat tgccgaagaa
    22921 cgaatgatcg ctccgcagct gccagagtgc attctgcacg aactcaccga gcgcccgcac
    22981 ccgttcccgc tcggcatcga cctaatcctg acctgcggcg agcgcctgct ggctatcccg
    23041 cgaaccactc atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gaaaaggcga
    23101 tcgacgccgc ccacgccctg caagagagcc gacgccgggg cgataccgat ttgcccgaac
    23161 tgagcgtcgc ccagattgaa cagcagctta acctcgcggt ggatcgcgtg atgacagaag
    23221 gcggcattgc cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg
    23281 tcgaagcgat tttcctgcta cgcgcctacc gcaccacgct gacgaaactg gcggtaagcg
    23341 agccgctcga caccacggag atgcgtctcg aacggcgtat ttcagcggtt tataaagata
    23401 ttcccggcgg ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca
    23461 ctctgctggc aaacggcgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt
    23521 cgccgcacgt tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg
    23581 gagcacagcc ggatgacatc acccgcacgc cgccggttta cccctgttca cgttcttccc
    23641 gtttgcagca gttaatgcgc ggcgacgaag gctatttgct ggcgctggct tactccaccc
    23701 agcgcggtta cgggcgcaac cacccgttcg cgggcgagat ccgcagtggt tacatcgacg
    23761 tgtcgattgt gccggaagag ctgggatttg cggtaaacgt cggcgaactg ctgatgaccg
    23821 agtgtgaaat ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg
    23881 gctacgggct ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc
    23941 gtgcgttgca agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt
    24001 tcgtgctggc gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc
    24061 cgcactacgt cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga
    24121 accatggcta atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg
    24181 atccgccgcg ccatcttaaa agcggtggcg atccccggtt atcaggtacc gtttggcggg
    24241 cgcgagatgc cgatgccgta cggctgggga actggtggta tccagctcac cgccagcgtg
    24301 attggcgaaa gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc
    24361 gtgtcgattc gcaacttctt taagcgcgtg accggggtaa acaccactga atgtacggac
    24421 gatgcgacgc ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag
    24481 atcattatct tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg
    24541 gagacccgca ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa
    24601 gatatcgccc gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg
    24661 cgctacgtga tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg
    24721 cccgccctgc agctgttcgg cgcagggcgc gagaagcgca tctatgcggt gccgccgttt
    24781 acccgcgtgg aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag
    24841 ccatgcgcca tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc
    24901 ggaaaccgca tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa
    24961 agccaatgaa tcaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca
    25021 aaggctttag cgatgtctct tttgatttat ggccggggga agtgctgggc attgtcgggg
    25081 aatccggctc cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc
    25141 agggggagat tcactatgaa aaccgttccc tctatgcaat gagcgaggcc gaccgccgtc
    25201 gcctgctgcg caccgaatgg ggcgtggtgc atcagcatcc gctcgacggc ctgcgccgcc
    25261 aggtgtcggc aggcggcaat atcggcgagc ggctgatggc gaccggggcg cgacattacg
    25321 gcgatattcg cgctaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgta
    25381 tcgacgacct gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca
    25441 acctggtgac gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt
    25501 cggtgcaggc ccgcctgctc gacctgctgc gcggactggt ggtggagctg aacctcgcgg
    25561 tagtgattgt cacccacgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga
    25621 tgaagcaggg gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc
    25681 atccgtatac acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg
    25741 gcgtaaacgc cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag
    25801 cgtcgcatcc gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa
    25861 aaccttcatc ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaacc gcgcctcgct
    25921 catcgtcaac gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gaaaatcaac
    25981 cctgctacgc tcgctgtacg ccaactatct gcccgacgaa gggcaaatcc agatcaaaca
    26041 cggtgacgaa tgggtcgatc tggtcaccgc gccagcgcgc aaagtggtgg agatccgcaa
    26101 aaccaccgtt ggctgggtga gccagtttct gcgcgtcatc ccgcgtattt cggcgctgga
    26161 agtggtgatg cagccgctac tcgataccgg cgtaccgcgt gaagcctgcg ccgctaaagc
    26221 cgcgcgtctg ctcacccgcc tgaacgtgcc ggaacgcctg tggcatctgg cgccatcgac
    26281 cttctccggc ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc
    26341 cattctgctg ctcgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgccgtagt
    26401 cgagctgatt cacgaagcca aagcccgtgg cgcggccatc gtcggcatct tccacgacga
    26461 agccgtacgt aatgacgttg ccgaccgcct gcacccaatg gaagcctctt catgattatc
    26521 aataacgtca aattggtgct ggaaaacgaa gtggtacacg gttcactgga ggtgcaggac
    26581 ggcgaaatcc gcgcctttgc cgaaagccag agccgcctgc cggaggcgat ggacggcgaa
    26641 ggcggctggc tgctgccggg gctgattgag ctgcataccg ataatctgga taaattcttc
    26701 accccgcgcc cgaaagttga ctggcctgcc cactcggcga tgagcagcca cgacgcgctg
    26761 atggtggcga gcggcatcac caccgtactg gatgccgtgg caattggcga cgtgcgcgac
    26821 ggcggcgatc ggctggtgaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa
    26881 cgcggcgtca accgcgccga gcaccgtctg catctgcgct gcgaactgcc gcatcacacc
    26941 acgctgccgc tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg
    27001 gaccactcgc cgggccagcg ccagttcgcc aatcgcgaga agtatcgcga atattatcag
    27061 ggcaaatact ccctcactga tgcgcagatg cagcagtacg aagaagagca actggcgctc
    27121 gccgcacgct ggtcgcagcc gaatcgcgaa tccatcgccg ccctgtgccg cgcgcgaaaa
    27181 attgcgcttg ccagccacga tgacgccacc cacgcccacg ttgctgaatc tcaccagctt
    27241 ggcagcgtga tcgccgaatt tcccaccacg ttcgaagcgg cggaagcctc gcgcaagcat
    27301 ggcatgaacg tgctgatggg cgcgccgaat attgtgcgcg gcggctcgca ctccggcaac
    27361 gtggcggcca gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac
    27421 cccgccagcc tgctcgatgc ggcatttcgc gtcgccgatg acgagagcaa ccgctttacg
    27481 ctgccgcagg cggtgaagct ggtgactaaa aatccagcgc aggcgcttaa tctccaggat
    27541 cgcggggtga ttggcgaggg caaacgcgcg gacttggtgc tggcgcatcg caagggcaat
    27601 cacattcata tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac
    27661 tgatttggtt aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc
    27721 tgcgggaaca aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgccg
    27781 gaagcgaaaa ccatatcgct ctgagcgagc aggagttttt tacccgcgcg gggcagaacc
    27841 tactggcctt aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagatcgacc
    27901 tctggctgca cgccggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg
    27961 cgcgggcgcg ctatcaatcg gcgctgctgc ccgtctgttt acaggtttcg ccggagatcc
    28021 tccgccagcg cctggaaaac cgtggccgtg aaaacgccag tgaaattaac gcccgcctgg
    28081 cgcgcgccgc ccgctatact ccacaggatt gccatacgct caacaatgac ggcagcctgc
    28141 gccagtcggt cgacacgctg ctgacgctga ttcatcagaa ggagaaacac catgcctgct
    28201 tgtgagcttc gcccggccac gcagtatgat accgacgcgg tttacgcgct gatttgtgag
    28261 ctaaaacagg cggagtttga ccaccacgca tttcgcgtgg gctttagcgc caatctgcgc
    28321 gacccgaaca tgcgctatca tctggcgctg ctggatggcg aggttgtcgg catgatcggc
    28381 ctgcatttgc agtttcatct gcatcatgtc aactggatcg gcgaaattca ggaactggta
    28441 gtgatgccgc aggcgcgcgg cctgaacgtc ggtagcaagt tgctggcgtg ggcagaagaa
    28501 gaagcccgcc aggccggggc cgaaatgacc gagctttcga ccaacgtgaa gcgccacgac
    28561 gcgcaccgtt tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg
    28621 ctgtaacatg agtctgaccc tcacgctcac cggcaccggc ggcgcacagg gcgttccggc
    28681 atggggctgc gagtgcgcgg cctgcgaccg agcgcggcgc tcgccgcagt atcgccgcca
    28741 accgtgcagc ggcgtagtga agtttaacga ggcgatcacc ctgatcgacg ccgggctgca
    28801 cgatctcacc gatcgctggt ctcccggatc gttccagcag tttttgctga cgcattatca
    28861 tatggatcac gtccaggggc tgtttccgct gcgctggggc gtgggcgatc cgatcccgat
    28921 ctacggcccg ccggatgaac agggctgcga cgatctgttt aaacatcccg ggctgcttga
    28981 tttcagccac acggtggaac cgtttgtggt gtttgatttg caggggttgc aggtcacgcc
    29041 gctgccgctt aaccactcaa aactcacttt cggctattta ctggaaacgg cgcacagccg
    29101 ggtggcgtgg ctgtctgaca ccgctggctt gccggaaaaa acgctgaaat ttttacgcaa
    29161 taatcagccg caggtaatgg tgatggattg cagtcacccg ccgcgcgcgg atgcaccgcg
    29221 taatcactgt gatttaaata ccgtgcttgc gctgaatcag gttatccgct cgccacgggt
    29281 gattctgacc catatcagcc accagtttga tgcgtggctg atggaaaacg cactaccgtc
    29341 agggtttgag gtggggtttg atgggatgga gattggggtg gcgtgatgag agggaatgtg
    29401 cgcgctggcc ccctcaccct aaccctctcc ccagaggggc gaggggaccg attgtgctca
    29461 atattgaata ttgcgctcgt tttctccctc tccccattgg ggtgaggggc gatgcctgct
    29521 ccatacccaa cctcatcgcc catactcatc ttccattctc cgctcttcat cctccagttg
    29581 ccgacgctcc tgatcaagct ggcgctggcg atcgtccagc tgcctgcggc gatcttcaaa
    29641 ctggcggcgg cggtcgtcat attgtctgcg ccgatcgtcg ctcacttcac gctgccagcc
    29701 gtcgtcgcgc gaatcttcat agtctcgccc acggtcaggg ttataagcgt cattaatcgc
    29761 ctgctgaata ttaccaatag tatcgtcgat aatatcagcc tgggccggaa cggtggacag
    29821 cgtgagcagg gtgaataaaa gaaatagcgg aaagcgtttc attagccaac ctcaaaaaga
    29881 aactctatcc acattagtca ttactcatcc atgcaagtag tggatgaatc tcaatttctc
    29941 cgctgctcta ttgccgtaat cgcctccacg cgttgttgat gacgaccgcc ttcgtactgt
    30001 gcgcccagcc acgcatccac aatcattttt gccagttcga ggccaaccac tcgtgaacca
    30061 aaagccagtg caacttcact ggcataatgc ggataatcag tgcgctctga cgacctggtg
    30121 tctttatcaa tcacctcact cattgaccat gaataaaagg ctggcacagc aacgcagaat
    30181 cctgcggtgc cagcgacatg accgcccatt gctccggtac gttttttaaa aagaaacacc
    30241 ggttgcattg ataaattgtg ctcagtattt gccgaagtac aatatatata ataaatatat
    30301 aaaacacctc atgttattta cagagataat aaattcgcgt aattttccct cttcagcaca
    30361 aatgcctaat aaaatggatc gttcagctca cacttccact tattaaagtc gcaaattccc
    30421 aattatgata accccagctt taaatgacgt attttgcatg gattacatat ttacgacata
    30481 aatgataaat ggagatttac atgaataact ctcggttatt ccgtttgagc agaattgtta
    30541 ttgcgttaac tgccgccagc ggcatgatgg taaataccgc taacgcgaaa gaggaagcga
    30601 aagccgccac tcaatatacc caacaggtta atcagaatta cgccaaatca ttaccgttta
    30661 gcgatcgtca ggattttgac gacgctcagc gtggatttat cgctccgctg ctggatgaag
    30721 gtattctgcg cgatgctaat ggcaaaccat attatcgcgg ggaagattat aaatttgata
    30781 tcaatgcgcc cgcgcccgaa accgttaacc ctagcctgtg gcgtcagtcg cagttaaacg
    30841 gtatttccgg cctgtttaaa gtcaccgaca gaatgtacca ggttcgcggt caggatatct
    30901 ccaacatcac ctttatcgaa ggtgataccg ggattattgt catcgacccg ctggtgacgc
    30961 caccgagcgc aaaagccgcc cttgaccttt acttccaaaa tcgcccgcaa aaaccgattg
    31021 ttgcggttat ttatacccac agccataccg accactatgg cggcgtgaaa ggcattatct
    31081 ctgaagctga tgtgaaatcc ggtaaagtgc aggttatcgc ccctgctggc tttatggacg
    31141 aagccatcag cgaaaacgta ctggcgggta atattatgag ccgccgtgca ctttactcct
    31201 acggcctgct gctggcgcat aaccctcagg gtaacatcgg caatggtctt ggcgtaacgc
    31261 tggcatcggg ctacccgagc atcatcgcac cgaacaaaac catcaccaaa accggtgaga
    31321 agatgattat cgacggcctg gagtttgact tcctgatgac cccaggtagc gaagcaccag
    31381 ccgaaatgca cttctatatt ccggccctga aagcgctgtg taccgccgag aacgccacgc
    31441 ataccctgca caacttctac actctgcgcg gcgcgaaaac ccgcgacacc agcaagtgga
    31501 ccgagtatct gaacgaaacg ctggatatgt ggggtaacga cgcggaagtc ctgtttatgc
    31561 cgcacacctg gccggtctgg ggcaataagc atatcaatga ttatattggt aaatatcgcg
    31621 atactatcaa gtacattcac gaccagaccc tgcacctggc gaaccagggc tacaccatga
    31681 atgaaatcgg cgacatgatt aaactgccgc ctgcacttgc caataactgg gccagccgtg
    31741 gctattacgg ttctgtcagc cacaacgccc gcgcggtgta taacttctat cttggctatt
    31801 acgacggtaa cccggctaac ctgcatccgt atggtcaggt ggagatgggt aaacgttacg
    31861 tgcaggcgct gggcggttct gcccgtgtca tcaacctggc gcaagaagcg aacaagcaag
    31921 gtgattaccg ctggtcggca gaactgctga aacaggtgat tgccgccaac ccgggtgacc
    31981 aggtcgcgaa gaatctgcaa gcgaataact ttgaacagct gggctatcag gccgagtccg
    32041 ccacctggcg cggtttctac ctgaccggcg cgaaagagct gcgcgaaggg gtgcataagt
    32101 tcagccacgg caccatcggt tccccggaca ccattcgcgg gatgtcggtc gaaatgctgt
    32161 tcgactttat gtccgttcgc ctcgatagcg cgaaagccgc gggtaaaaat atcagcctga
    32221 acttcaatat gagcaatggc gataacctca acctgacgct gaacgatagc gtgcttaact
    32281 accgtaaaac actgcaatcc caagctgacg cctctttcta catcagccgt gaagatctgc
    32341 acgccgtgct gaccgggcaa gccaaaatgg cggatctggt aaaagcgaag aaagccaaaa
    32401 ttattggcaa tggcgcgaaa ctggaagaaa ttatcgcctg tctggataat ttcgatttgt
    32461 gggtgaatat cgtaacccca aattaatgtc actcgctttt gggggcgctg aagcccccaa
    32521 aagcatttat tttaaaaata tatacagcca attaataaat aaaatatata agttatgaat
    32581 gcggtgactg aataaagcta tttttattca tcgagaaaat agccatactg acgccttcaa
    32641 tggatattga tattcactac agatattgtt cgcagcagac aggatgtatt taatatgcca
    32701 acagttctct ctcgcatggc gatgcaactc aaaaaaacag cctggataat tcccgtcttc
    32761 atggtttcgg gatgctcatt atctccggca atcccggtga tcggtgctta ttatcccagc
    32821 tggtttttct gcgccattgc cagccttatt ttgatgctca tcacgaggcg agttattcag
    32881 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
    32941 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
    33001 ccgaaaaaag ctcctcgcag taaattccct gctctgttag tggttgcatt ggcgctggtt
    33061 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
    33121 tcagcagata ccattgatgt ggtgccggaa gtcagcggcc gcattgtaga actggcggtc
    33181 accgacaacc aggcagtcaa acagggcgat ttgctgttcc gcattgaccc gcgcccgtac
    33241 gaagccaatc tggcgaaagc tgaagcctcc ctcgcggcgc tggataagca aattatgctc
    33301 acccagcgta gcgttgatgc gcaacagttt ggtgccgact cgattaatgc cacggtagaa
    33361 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgtaccga gccattactg
    33421 aaagagggtt ttgtctcagc ggaagacgtt gaccgtgcaa gaactgcgca gcgcgccgca
    33481 gaagccgatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
    33541 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaactg
    33601 catctggaaa tggcgaccgt tcgcgcgccg tttgatggcc gggtcatttc cctcaaaacc
    33661 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctaatcga cactcgtcac
    33721 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
    33781 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
    33841 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc gaaagtttct
    33901 cgctctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
    33961 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
    34021 tgagcgcgct caactccctg ccattaccga tggtcaggct gctggcgttc tttcatgaag
    34081 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
    34141 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
    34201 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
    34261 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
    34321 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggccgcatg tttttgatgc
    34381 gcacccatcg gctgggactg gtctttttcg ctgtcgccat tgtcgccatt tacgggcaaa
    34441 ccttccccgc catgctcgac tatccggaag tggtcgtgcg cttaacgctg tggtgtatcg
    34501 ttgttggcct ctatccaacc ttgctgatga cgttaatcgg cgtgctgtgg tttcccagtc
    34561 gtgccatttc gcaaatgcat caggcgctta atgatcggct tgatgatgcc attagtcacc
    34621 tgacagacag cctcgcaccg ctacccgaaa cgcggattga aagagaggcg ctggcgctgc
    34681 aaaaactgaa tgtcttttgt ctcgcggacg atgccaactg gcgaactcaa agcgcatggt
    34741 ggcaaagctg cgtggcaacg gtaacctaca tttactcgac gctgaatcgc tacgatccca
    34801 cctcttttgc tgattctcag gcaattattg aatttcggca aaaattagcc tcagaaatca
    34861 acaagctgca gcatgccgtt gctgaaggtc agtgctggca aagcgactgg cggatcagtg
    34921 aaagtgaagc ggtggcggca cgggaatgta acctggagaa tatctgccag acgttgttac
    34981 aactgggcca gatgaacccg aatacgccgc caacgcccgc cgccaaaccg ccatcaatgg
    35041 tcgccgatgc ttttaccaat ccagactata tacgctacgc ggtaaaaaca ttgctcgcct
    35101 gtttgatctg ttacaccttt tacagcggcg tggactggga aggcattcac acctgtatgc
    35161 tgacctgcgt gatcgtctct aatccaaatg tcggttcgtc gtaccagaag atggtgctgc
    35221 gttttggcgg ggccttttgc ggcgcgattc tggcgctgtt attcacgcta ctggtcatgc
    35281 cctggctgga caatattgtc gaattgctgt ttgtgctggc accgattttc ctgttgggcg
    35341 catggattgc caccagctct gaacgctctt cttatatcgg cacacagatg gtggtcacct
    35401 tcgcgctcgc cacgctcgaa aacgtttttg gcccagtgta cgacctggtg gaaattcgcg
    35461 atcgcgccct gggtatcatc attggtaccg tggtgtccgc ggtgatttac acctttgtct
    35521 ggcctgaaag tgaagcgcgc acgctgccgc aaaaactggc tggcgcgctg ggtatgttaa
    35581 gtaaagtaat gcggatccca cgccagcagg aagtcacggc tctgcgcact tatctgcaaa
    35641 ttcgtatcgg tctgcatgcg gcgtttaatg cctgtgaaga gatgtgccaa cgcgtggcgc
    35701 tggagcgtca actggacagc gaagaacgcg ccttactgat tgaacgttcg caaacggtta
    35761 ttcgtcaggg ccgcgatatc cttcacgcct gggatgccac ctggaattcg gcgcaggcgc
    35821 tggataacgc actacagccg gacagagcag gtcagtttgc cgacgccctg gaaaaatacg
    35881 ctgccggtct ggcaaccgca ctcagccgtt ctcctcaaat aacgcttgaa gagacacccg
    35941 cctcgcaggc catcctgccc accttattaa aacaggagca acacgtctgc cagcttttcg
    36001 cccgcttgcc agactggaca gccccggcat taacgcccgc cacggaacag gcacaaggag
    36061 ccacgcaatg atcaatcgtc aactttcacg tctgctgttg tgcagcattc tcggcagcac
    36121 gacgctgatt tccggctgtg ccttggtacg caaggattct gcgcctcatc aacagctcaa
    36181 accggaacaa atcaaactgg ccgatgatat tcatcttgcc agctccggct ggccgcaggc
    36241 gcagtggtgg aaacaactca atgacccgca gctggatgcg ctgatccaac ggacgctaag
    36301 tggttcacac accctcgccg aagcgaaact gcgggaagaa aaagcgcagt cccaggccga
    36361 tttgttagat gccggttcac aattacaggt cgcagcgtta gggatgctca accgccaacg
    36421 cgtctcggcg aacggctttt taagccctta tgcgatggat gcgcccgcac tgggtatgga
    36481 cgggccgtac tatacggaag ccacagtagg tttgtttgcc ggactggatc ttgatttgtg
    36541 gggtgtgcat cgctcagcgg ttgccgccgc cattggcgcg cataatgccg cgctggcaga
    36601 aaccgcagca gtagagctat cgctgaccac gggcgtagcg cagctttatt acagtatgca
    36661 ggccagctat cagatgctcg atctgttaga acaaactcgc gatgtgattg attacgcggt
    36721 gaaagcgcac cagagtaaag tggcgcacgg tctggaagca caagtgcctt tccacggcgc
    36781 gcgggcacag attctggcgg tcgataaaca aattgctgcc gtcaaagggc aaatcaccga
    36841 aacgcgagaa tctctgcgtg cattgattgg cgcgggggcc agcgatatgc cggagatcaa
    36901 accggtggca ttaccacgag tccagaccgg cattccggcg acactctctt atgagttgct
    36961 cgccagacgc ccggatctgc aagccatgcg ctggtatgtt caggcgtcat tagatcaggt
    37021 ggattccgcg cgggcgctgt tctacccgag ctttgatatc aaagcgtttt tcggccttga
    37081 tgccatccac ctggatacct tattcaaaaa aaccagtcgc cagttcaact tcatcccggg
    37141 tctgaaattg ccgctgtttg acggtggacg gttgaatgcc aatctcgaag gcacgcgcgc
    37201 cgccagcaac atgatgattg aacgttacaa ccagtcagta ctgaacgcgg tgcgtgacgt
    37261 tgccgtcaac ggcacgcgtc tgcaaacgct caacgacgag cgagaaatgc aggctgaacg
    37321 cgtggaagca acgcgcttca cccagcgcgc tgccgaggcc gcctatcagc gcggcttaac
    37381 cagccgctta caggccactg aagcccggtt gccagtactt gccgaagaga tgtcattact
    37441 gatgctggac agccgccggg tgatccaaag cattcagttg atgaaatcgc tgggcggcgg
    37501 gtatcaggcg gctcccgtcg tcgagaaaaa ataaaatgtc tgccgcgtga tggctgtcac
    37561 gcggtatttc gtttcgtcac gtcaaaactg acgacagcct gtttttcgtc agagttttga
    37621 ataaatagtg cccgtaatat cagggaatga ccccaaataa aatgtggcat aaaagatgca
    37681 tactgtagtc gagagcgcgt atgcgtgatt tgattaactg gagcgagacc gatgaaaaaa
    37741 gtcgtcacgg tttgccccta ttgcgcatca ggttgcaaaa tcaacctggt cgtcgataac
    37801 ggcaaaatcg tccgggcgga ggcagcgcag gggaaaacca accagggtac cctgtgtctg
    37861 aagggttatt atggctggga tttcattaac gatacccaga tcctgacccc gcgcctgaaa
    37921 acccccatga tccgtcgcca gcgtggcggc aaactcgaac ctgtttcctg ggatgaggca
    37981 ctgaattacg ttaccgagcg cctgagcgcc atcaaagaga agtacggtcc ggatgccatc
    38041 cagacgaccg gctcctcgcg tggtacgggt aacgaaacca actatgtaat gcaaaaattt
    38101 gcgcgcgccg ttattggtac caataacgtt gactgctgcg ctcgtgtctg acacggccca
    38161 tcggttgcag gtctgcacca atcggtcggt aatggcgcaa tgagcaatgc tattaacgaa
    38221 attgataata ccgatttagt gttcgttttc gggtacaacc cggctgattc ccacccaatc
    38281 gtggcgaatc acgtaattaa cgctaaacgt aacggggcga aaattatcgt ctgcgatccg
    38341 cgcaaaattg aaaccgcgcg cattgctgac atgcacattg cactgaaaaa cggctcgaac
    38401 atcgcgctgt tgaatgcgat gggccatgtc attattgaag aaaatctgta cgacaaagcg
    38461 ttcgtcgctt cccgtacaga aggctttgaa gagtatcgta aaatcgttga aggctacacg
    38521 ccggagtcgg ttgaagatat caccggcgtc agcgccagtg agattcgtca ggcggcacgg
    38581 atgtatgccc aggcgaaaag cgccgccatc ctgtggggca tgggtgtaac ccagttctac
    38641 cagggcgtgg aaaccgtgcg ttctctgacc agcctcgcga tgctgactgg taacctcggt
    38701 aagccgcatg cgggtgtaaa cccggttcgt ggtcagaaca acgtgcaggg tgcctgcgat
    38761 atgggcgcgc tgccggatac gtatccggga taccagtacg tgaaagatcc ggctaaccgc
    38821 gagaaattcg ccaaagcctg gggcgtggaa agcctgcctg ctcataccgg ttatcgcatc
    38881 agcgagctgc cgcaccgcgc agcgcatggc gaagtgcgtg ccgcgtacat tatgggcgaa
    38941 gatccgctgc aaactgacgc ggagctgtcg gcagtacgta aagcctttga agatctggaa
    39001 ctggttatcg ttcaggacat ctttatgacc aaaaccgcgt cggcggcgga tgtcattttg
    39061 ccgtcaacgt cgtggggcga gcatgaaggc gtgtttactg cggctgaccg tggcttccag
    39121 cgtttcttca aagcagttga accgaaatgg gatctgaaaa cggactggca aatcatcagt
    39181 gaaatcgcca cccgtatggg ttatccgatg cactacaaca acacccagga gatctgggat
    39241 gagttgcgtc atctgtgccc ggatttctac ggtgcgactt acgagaaaat gggcgaactg
    39301 ggcttcattc agtggccttg ccgcgatact tcagatgccg atcaggggac ttcttatctg
    39361 tttaaagaga agtttgatac cccgaacggt ctggcgcagt tcttcacctg cgactgggta
    39421 gcgccaatcg acaaactcac cgacgagtac ccgatggtac tgtcaacggt gcgtgaagtt
    39481 ggtcactact cttgccgttc gatgaccggt aactgtgcgg cactggcggc gctggctgat
    39541 gaacctggct acgcacaaat caataccgaa gacgccaaac gtctgggtat tgaagatgag
    39601 gcattggttt gggtgcactc gcgtaaaggc aaaattatca cccgtgcgca ggtcagcgat
    39661 cgtccgaaca aaggggcgat ttacatgacc taccagtggt ggattggtgc ctgtaacgag
    39721 ctggttaccg aaaacttaag cccgattacg aaaacgccgg aatacaaata ctgcgccgtg
    39781 cgcgtcgagc cgatcgccga tcagcgcgcc gccgagcagt acgtgattga cgagtacaac
    39841 aagttgaaaa ctcgcctgcg cgaagcggca ctggcgtaat acagtccttt ctacagcctc
    39901 ctttcggagg ctgttttttt atccattcga actctttata ctggttactc cctacccaat
    39961 cgtattatca aaatgaaaaa aattatcgca ttgatgttgt ttttgacatt ctttgcccac
    40021 gccaacgact ccgagcctgg cagccagtat ttaaaggcag cagaggccgg ggaccgacgc
    40081 gcacaatatt ttcttgccga cagctggttt agctccggcg atttgagcaa agccgaatat
    40141 tgggcacaga aagccgccga cagcggtgat gctgatgcct gcgcgctgct ggcgcagatc
    40201 aaaatcacca atccggtcag tctggactat ccacaagcaa aagttcttgc agagaaagcg
    40261 gcgcaagcgg gcagtaaaga aggtgaagta acgctggcgc atattctggt aaatactcaa
    40321 gcgggtaaac cggattatcc aaaggctatt tcgctgttag aaaacgcctc ggaagatctg
    40381 gagaacgact ctgccgtcga tgcccaaatg ctgcttggtt tgatttacgc caacggcgtg
    40441 ggcattaagg ccgacgatga caaggcaacc tggtatttca aacgcagctc tgcaatttcc
    40501 cgaaccggtt attccgagta ctgggcggga atgatgttct taaacggtga agaaggtttt
    40561 atcgagaaga acaagcaaaa ggcgctgcac tggttgaacc tgagctgtat ggaagggttt
    40621 gataccgggt gtgaagagtt tgaaaaatta accaacggtt aaggtaggcc tgataagacg
    40681 cggcaagcgt cgcatcaggc attcacacat tgccgggtgg atatccccgg caatcttcaa
    40741 ttattgatcc gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt
    40801 tttgcggtca aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac
    40861 cacgttcagc gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag
    40921 accttccagc ggaataccta cgctacccag cgttgccagc agcaccacaa acgacacgcc
    40981 cggcacgcca gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg
    41041 ccagatggac aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata
    41101 cagcgtcgaa ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga
    41161 cgccggtgct ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct
    41221 ggcagtggag tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct
    41281 taacccgcac aggcgcgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa
    41341 atgcaccagc agcaccagtt tcgccagtgg ccacagagaa gagaaaccaa agttagccac
    41401 cgtcaccgca atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac
    41461 tttaaacatg gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc
    41521 gggcagggaa gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc
    41581 gcctttcgcc atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat
    41641 gccgtgggaa ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt
    41701 cgccagctgc gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc
    41761 caaaatgatg gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg
    41821 cccgagctgt ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat
    41881 cggcacaaca atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt
    41941 gacgaccagc cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc
    42001 cagcaccata gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat
    42061 aacttcctca atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag
    42121 ggcatgaatt gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt
    42181 gcgcggtatg ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa
    42241 cgcgagttta tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt
    42301 aatctgcaat aatactgatt agtgattatt tttggcatat gttcattcga ttatttttta
    42361 tgcagctcaa tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt
    42421 tttgccatat taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc
    42481 atcacttttt gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa
    42541 gccagcagca tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt
    42601 gcgcaagtct gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc
    42661 gcatacagtt cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa
    42721 ttgctgtaat cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt
    42781 tgtggattag cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg
    42841 ctggcaaact gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat
    42901 tttgggctta acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc
    42961 agcattttca ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca
    43021 gtgccatcag caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta
    43081 acggtgggtt atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga
    43141 cttcgttctt cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg
    43201 gcgcattgga ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa
    43261 tatttaacgc ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acgacctgag
    43321 cgcgcgcaaa acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc
    43381 ttcccccgcc atccgcttaa caatgcgccc gcaatcatca acaatcctcc accccagatc
    43441 cagcgcacac cgctttgtac atacaaacga aaagcgtaac gatccgctcc ggttttttca
    43501 cccatgaccg cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc
    43561 tgacggcgtg cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg
    43621 tcaaacagcg ccactatcgc tttttcgctg gtgtaattgc ctttggcttg cagatcgagg
    43681 cgctcaaaac ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg
    43741 ctgatctcct gacggctgac gctggaaacc acgatccctg cggcaaataa cagcacgccc
    43801 gcatgagcga ccagcgccgg aagctgcgcg cgtttgccag agacaaacgt cgccagcaca
    43861 atcaccacca gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca
    43921 acggagaggc gtccccagcc tagcagaccg taaatcatcg gataaagcgt tcccaccagc
    43981 acgatcagta gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat
    44041 aaccccgaaa aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa
    44101 gacgcaaggc taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt
    44161 gcgaacgcat gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac
    44221 agcatcagag taactatcgc cagcaacagc gaccagtggc aaaaaatccc ccgctggcgt
    44281 gtcagggata aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcattt
    44341 tccaccggat cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa
    44401 ccgaggatga tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt
    44461 cgggcgctgg ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct
    44521 accatcaaac cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt
    44581 tgcagcatcg gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga
    44641 tcggaccaca gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctaac
    44701 gtcagcggaa atagcggatc ggtttgctgc cgataatgcc aggcaaacag cgcgctccag
    44761 gcggaaagca gcagcaccca aagcagcagc gaaccttcat ggccgcccca caccgctgcc
    44821 agcttgaatt cccacgacag caggctatag ctatgttgcg cgacataaat gaccgagaaa
    44881 tcgctgatga gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc
    44941 gccagaatgc cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca
    45001 aaggccgtca acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg
    45061 acttcaggaa ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg
    45121 tccggcgtag agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc
    45181 cgccagcacg gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac
    45241 aatcagcccc agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa
    45301 tgccgccacc agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc
    45361 caccagcagg aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc
    45421 ttccgtggaa tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc
    45481 accggacgag atgccggaga agaggaacag caccggcagg atcgggttat tgaggaacgg
    45541 atacgatttc agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac
    45601 cagcatcagc gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac
    45661 gatccccagc ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt
    45721 cgccagccac agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat
    45781 caccgaggta aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg
    45841 gaagacgagg atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg
    45901 cagcaacgta ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag
    45961 cgtcaccaga ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca
    46021 caccagcgat tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta
    46081 aacggtacgc ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc
    46141 ttctggcgca gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc
    46201 gccttcgtcg ggcaagcctc aacacacgcg ggcagcttac cggcctgcaa attggtttta
    46261 cggcagaaat cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac
    46321 ggacaggcgg cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc
    46381 ccgctagcgg catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga
    46441 tcgcagtgct ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc
    46501 ggctcgctac gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga
    46561 caagcatcca tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa
    46621 cgcaccccat tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct
    46681 gccagcacgc cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa
    46741 gcaacggaac agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga
    46801 cgcaaatctt aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca
    46861 gggaatggca gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt
    46921 tttgcaactg ttccggcaga tgacaggaca tacagacgct gttctgctct ccaaccttgt
    46981 acatcggctc gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatggct
    47041 ggccgtgaca gttggtgcag gtgaccggca gtttgttatt cgggttgata acggaagcat
    47101 gtttgccatg cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt
    47161 tacgctgctg ggtaacttcg tagcgatcat cagatgcctg cgccgcaggg gtagcggtaa
    47221 ttccgttcag gctccacaac aggcccgacg ccagcacccc ggcagttaat aacgaacgta
    47281 atacgctcat attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg
    47341 gcttaacaga ccgtttttac gtgcctgttc ttcccactgc gggatcaccg ttttgatgaa
    47401 gtcctgcttc tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc
    47461 tttggttgag atatccggga tctggatttc atgggtgatg cctttggtcg ccagcaggcg
    47521 cgccagtttg gtgcgtgcat ccgccgcttt atccatcgcc gtaccgagca tccgtaaacc
    47581 ttcttccggt gcgtgcatat gaatgccgtg ggaagcgatc gccagatccc agcgccactg
    47641 ggcatgacgg atatcgtcct gaattggctt catttcagct tccgtcgcgc ctgcatccag
    47701 cgctgctttc gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc
    47761 gttaatcgac tgcttacgtt ccgcgaccac tttttgcaag gcagctttgt cctgggtatg
    47821 gcagttcgca caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtata
    47881 gagtttgcct tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt
    47941 tttaccgtga atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg
    48001 cgttttcgac agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat
    48061 attttcgact ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa
    48121 gtaatactcc acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt
    48181 ctcaaaaggt ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa
    48241 ctccggtttg cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa
    48301 gttgttgacg atttccgggc cgccgcgcgc ccacttaccg tggaagtagc catcttcgcc
    48361 gtctttctgg atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag
    48421 cggaccatct tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac
    48481 agcaaaggca tgtccacgcg gcttgttgta atcgcgcgag aagggatacc ccgcccacag
    48541 gatcaccagc cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc
    48601 tttccaggag agatattgat cgggatgctg cggggcaaag gtttcattct tcgcttccac
    48661 agttacaggt tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa
    48721 aggaatcaat aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc
    48781 atccgaattg cgccattgtt acaatggcgg tttttattgt ttttcacgac agtaaccgca
    48841 cctacactgt catgacattg ctcgccccta tgtgtaacaa ataaccacac tgtgaatgtt
    48901 gtctttaatc aattgtaagt gcatgtaaaa taccacttta gagttagtta gtatcttcct
    48961 ctttttcaac agcaatgcat aactgcatgt tcctcaaaga attaatcaac ttttgttgct
    49021 gaccttcaaa aattaccctg ccgtttattt gcacaattct acttttgcgt gatctgtcgc
    49081 ccaaatacta aacaaaactg ccaatacccc tacatttaac gcttatgcca catattatta
    49141 acaccctaca aggagaacaa aagcatgagc caaattcaca aacacaccat tcctgccaac
    49201 atcgcagacc gttgcctgat aaaccctcag cagtacgagg cgatgtatca acaatctatt
    49261 aacgtacctg ataccttctg gggcgaacag ggaaaaattc ttgactggat caaaccttac
    49321 cagaaggtga aaaacacctc ctttgccccc ggtaatgtgt ccattaaatg gtacgaggac
    49381 ggcacgctga atctggcggc aaactgcctt gaccgccatc tgcaagaaaa cggcgatcgt
    49441 accgccatca tctgggaagg cgacgacgcc agccagagca aacatatcag ctataaagag
    49501 ctgcaccgcg acgtctgccg cttcgccaat accctgctcg agctgggcat taaaaaaggt
    49561 gatgtggtgg cgatttatat gccgatggtg ccggaagccg cggttgcgat gctggcctgc
    49621 gcccgcattg gcgcggtgca ttcggtgatt ttcggcggct tctcgccgga agccgttgcc
    49681 gggcgcatta ttgattccaa ctcacgactg gtgatcactt ccgacgaagg tgtgcgtgcc
    49741 gggcgcagta ttccgctgaa gaaaaacgtt gatgacgcgc tgaaaaaccc gaacgtcacc
    49801 agcgtagagc atgtggtggt actgaagcgt actggcggga aaattgactg gcaggaaggg
    49861 cgcgacctgt ggtggcacga cctggttgag caagcgagcg atcagcacca ggcggaaaag
    49921 atgaacgccg aagatccgct gtttattctc tacacctccg gttctaccgg taagccaaaa
    49981 ggtgtgctgc atactaccgg cggttatctg gtgtacgcgg cgctgacctt taaatatgtc
    50041 tttgattatc atccgggtga tatctactgg tgcaccgccg atgtgggctg ggtgaccgga
    50101 cacagttact tgctgtacgg cccgctggcc tgcggtgcga ccacgctgat gtttgaaggc
    50161 gtacccaact ggccgacgcc tgcccgtatg gcgcaggtgg tggacaagca tcaggtcaat
    50221 attctctata ccgcacccac ggcgatccgc gcgctgatgg cggaaggcga taaagcgatc
    50281 gagggtaccg accgttcgtc gctgcgcatt ctcggttccg tgggcgagcc aattaacccg
    50341 gaagcgtggg agtggtactg gaaaaaaatc ggcaacgaga aatgtccggt ggtcgatacc
    50401 tggtggcaga ccgaaactgg cggtttcatg atcacgccgc tgcctggcgc taccgagctg
    50461 aaagccggtt cggcaacacg tccgttcttc ggcgtgcaac cggcgctggt cgataacgaa
    50521 ggtaacccgc tggaaggcgc taccgaaggc agcctggtga tcaccgactc ctggccgggt
    50581 caggcgcgta cgctgtttgg cgatcacgaa cgttttgaac agacctactt ctccaccttc
    50641 aaaaatatgt atttcagcgg cgacggcgcg cgtcgcgatg aagatggcta ttactggatc
    50701 accgggcgtg tggacgacgt gctgaacgtc tccggtcacc gtctggggac ggcagagatt
    50761 gagtcggcgc tggtggcgca tccgaagatt gccgaagccg ccgtagtagg tattccgcac
    50821 aatattaaag gtcaggcgat ctacgcctac gtcacgctta atcacgggga ggaaccgtca
    50881 ccagaactgt acgcagaagt ccgcaactgg gtgcgtaaag agattggccc gctggcgacg
    50941 ccagacgtgc tgcactggac cgactccctg cctaaaaccc gctccggcaa aattatgcgc
    51001 cgtattctgc gcaaaattgc ggcgggcgat accagcaacc tgggcgatac ctcgacgctt
    51061 gccgatcctg gcgtagtcga gaagctgctt gaagagaagc aggctatcgc gatgccatcg
    51121 taacccacaa ttgccggatg cgagtcggta acggtttgta ggcctgataa gacgcgacag
    51181 cgtcgcatca ggcattgatt gccggatgcg gcgtaaacgc cttatccggc ctacattcgg
    51241 caagggttac ccgagcgtta accttctccc ataagggagc gggaattaaa acaatcccta
    51301 cattacctct ggagaatctg tgatgaatgg cactatttat cagcggatag aagacaatgc
    51361 gcatttcagg gagttagtcg aaaaacggca acggtttgcc accatcctgt cgattattat
    51421 gctggcagtt tatatcggct ttattttact gatcgccttc gcgcccggct ggctggcacg
    51481 ccgctgaatc cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg
    51541 atctcctttg ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt
    51601 aataatgaag tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg
    51661 ccacactccc tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc
    51721 caacgaactg gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta
    51781 cctactgggc atcaaaactc gtacgttctc gtagcgacta ctacaccgca ggcggcaata
    51841 tcactggctt ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct
    51901 tggggatctc cgcgctggtg tttacctccg gctatgacgg cttaatttac tcgctgggct
    51961 tcctggtggg ctggccgatc attttgttcc tgattgccga acgtctgcgt aacctggggc
    52021 gctacacctt tgccgatgtg gcctcttacc gtctgaaaca agggccgatt cgtattcttt
    52081 cggcctgtgg ttctctggtg gtggtggcgc tttaccttat cgcccagatg gtgggcgcag
    52141 gtaaactgat cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg
    52201 tgctgatgat gatgtacgtc ctgttcggcg gcatgctggc gaccacttgg gtacagatta
    52261 tcaaagccgt gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg
    52321 tcggctttag cttcaacaat ctgttcagtg aagcgatggc ggtacacccg aaaggtgtcg
    52381 acatcatgaa gccgggcggg ctggtgaaag atccgatctc cgcactctct ctgggtctgg
    52441 gactgatgtt tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg
    52501 atgcccgcga agcacgtaag agcgtgttct acgccaccgg gtttatgggc tacttctata
    52561 ttctgacctt tattatcggc ttcggcgcga tcatgctggt tggtgcgaat ccggaatata
    52621 aagacgcggc gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg
    52681 cagtgggcgg caacctgttc ctcggtttta tttcagcggt tgctttcgcc actatcctcg
    52741 cggtggttgc gggtctgacg ctggcgggcg catccgcggt ttcgcatgac ttgtacgcta
    52801 acgtcttcaa aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac
    52861 tgatcctcgg cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct
    52921 ttatggtggg gctggcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt
    52981 ctatgtactg gtcgaaactg accacgcgtg gcgcgatgat gggtggctgg ctggggctga
    53041 ttaccgcagt agtactgatg atcctcggcc cgacgatttg ggtacagatc cttggtcacg
    53101 aaaaagccat cttcccgtat gaatacccgg cgctgttctc tatcaccgtg gcattcctcg
    53161 gcatctggtt cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt
    53221 tccgcgcgca gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt
    53281 aatctctccc cttccccggt cgcctgaccg gggaatactc ttcctctcca gcatgcatca
    53341 ccttttccca aaatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg
    53401 ta
//
LOCUS       DAJTVK010000014        50053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000014 DAJTVK010000000
VERSION     DAJTVK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50053
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.014"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..1274
                     /locus_tag="OO372_000882"
                     /pseudo
     CDS             54..1274
                     /locus_tag="OO372_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="OO372_000883"
     CDS             1247..1876
                     /locus_tag="OO372_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6340461.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO372_000884"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="OO372_000884"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6340462.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..4234
                     /gene="hcxA"
                     /locus_tag="OO372_000885"
     CDS             3146..4234
                     /gene="hcxA"
                     /locus_tag="OO372_000885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6340463.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4244..4441)
                     /locus_tag="OO372_000886"
     CDS             complement(4244..4441)
                     /locus_tag="OO372_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6340464.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO372_000887"
     CDS             complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="OO372_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6340465.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO372_000888"
     CDS             complement(6909..7322)
                     /gene="entH"
                     /locus_tag="OO372_000888"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6340466.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO372_000889"
     CDS             complement(7325..8071)
                     /gene="entA"
                     /locus_tag="OO372_000889"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6340467.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO372_000890"
     CDS             complement(8071..8928)
                     /gene="entB"
                     /locus_tag="OO372_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6340468.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO372_000891"
     CDS             complement(8942..10552)
                     /gene="entE"
                     /locus_tag="OO372_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6340469.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO372_000892"
     CDS             complement(10562..11737)
                     /gene="entC"
                     /locus_tag="OO372_000892"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR6340470.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12112..13077
                     /gene="fepB"
                     /locus_tag="OO372_000893"
     CDS             12112..13077
                     /gene="fepB"
                     /locus_tag="OO372_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6340471.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO372_000894"
     CDS             complement(13158..14408)
                     /gene="entS"
                     /locus_tag="OO372_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6340472.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14519..15523
                     /gene="fepD"
                     /locus_tag="OO372_000895"
     CDS             14519..15523
                     /gene="fepD"
                     /locus_tag="OO372_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6340473.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15520..16512
                     /gene="fepG"
                     /locus_tag="OO372_000896"
     CDS             15520..16512
                     /gene="fepG"
                     /locus_tag="OO372_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6340474.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16509..17324
                     /gene="fepC"
                     /locus_tag="OO372_000897"
     CDS             16509..17324
                     /gene="fepC"
                     /locus_tag="OO372_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6340475.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO372_000898"
     CDS             complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="OO372_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6340476.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO372_000899"
     CDS             complement(18670..22551)
                     /gene="entF"
                     /locus_tag="OO372_000899"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR6340477.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO372_000900"
     CDS             complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="OO372_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6340478.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO372_000901"
     CDS             complement(22769..23971)
                     /gene="fes"
                     /locus_tag="OO372_000901"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6340479.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24214..26454
                     /gene="fepA"
                     /locus_tag="OO372_000902"
     CDS             24214..26454
                     /gene="fepA"
                     /locus_tag="OO372_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6340480.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26629..27249
                     /gene="entD"
                     /locus_tag="OO372_000903"
     CDS             26629..27249
                     /gene="entD"
                     /locus_tag="OO372_000903"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6340481.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO372_000904"
     CDS             complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="OO372_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6340482.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO372_000905"
     CDS             complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="OO372_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR6340483.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28466..29584
                     /locus_tag="OO372_000906"
     CDS             28466..29584
                     /locus_tag="OO372_000906"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6340484.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            29650..29898
                     /locus_tag="OO372_000907"
                     /pseudo
     CDS             29650..29898
                     /locus_tag="OO372_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            29963..30331
                     /locus_tag="OO372_000908"
     CDS             29963..30331
                     /locus_tag="OO372_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6340485.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30425..31078
                     /gene="nfsB"
                     /locus_tag="OO372_000909"
     CDS             30425..31078
                     /gene="nfsB"
                     /locus_tag="OO372_000909"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6340486.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31186..32433
                     /gene="ybdG"
                     /locus_tag="OO372_000910"
     CDS             31186..32433
                     /gene="ybdG"
                     /locus_tag="OO372_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6340487.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO372_000911"
     CDS             complement(32501..33877)
                     /gene="pheP"
                     /locus_tag="OO372_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6340488.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO372_000912"
     CDS             complement(33979..37122)
                     /gene="cusA"
                     /locus_tag="OO372_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6340489.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO372_000913"
     CDS             complement(37134..38357)
                     /gene="cusB"
                     /locus_tag="OO372_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR6340490.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO372_000914"
     CDS             complement(38373..38705)
                     /gene="cusF"
                     /locus_tag="OO372_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6340491.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO372_000915"
     CDS             complement(38729..40111)
                     /gene="cusC"
                     /locus_tag="OO372_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6340492.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40268..40951
                     /gene="cusR"
                     /locus_tag="OO372_000916"
     CDS             40268..40951
                     /gene="cusR"
                     /locus_tag="OO372_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6340493.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            40941..42389
                     /gene="cusS"
                     /locus_tag="OO372_000917"
     CDS             40941..42389
                     /gene="cusS"
                     /locus_tag="OO372_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6340494.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            43126..45027
                     /locus_tag="OO372_000918"
     CDS             43126..45027
                     /locus_tag="OO372_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6340495.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
     gene            45055..45516
                     /locus_tag="OO372_000919"
     CDS             45055..45516
                     /locus_tag="OO372_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCR6340496.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            45536..49947
                     /locus_tag="OO372_000920"
                     /pseudo
     CDS             45536..49947
                     /locus_tag="OO372_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
BASE COUNT        12012 a        13761 c        13091 g        11189 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3421 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3481 atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg
     3541 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3601 gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg
     3661 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3721 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3781 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3841 ggtggatgct attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg
     3901 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     3961 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga
     4021 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4081 ggagctggaa atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4141 gcgtccggtg gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
     4201 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
     4261 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
     4321 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4381 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4441 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4501 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt
     4561 agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
     4621 ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca
     4681 tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc
     4741 gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
     4801 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
     4861 ctgtctatca ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg
     4921 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc
     4981 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
     5041 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg
     5101 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
     5161 gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
     5221 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
     5281 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
     5341 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca
     5401 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta
     5461 atcgcaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
     5521 gccggagcca gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata
     5581 caggcggaaa ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg
     5641 ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
     5701 gcatggaagc ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta
     5761 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
     5821 ggacgcataa tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta
     5881 gagaggtagt cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg
     5941 tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
     6001 gggctttctg ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc
     6061 actgacactt cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc
     6121 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
     6181 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc
     6241 gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg
     6301 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
     6361 ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc
     6421 accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
     6481 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
     6541 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
     6601 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
     6661 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
     6721 ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac
     6781 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
     6841 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
     6901 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
     6961 tcatcgaaaa cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
     7021 acgccgcgta ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc
     7081 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
     7141 agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
     7201 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
     7261 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
     7321 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
     7381 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
     7441 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
     7501 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
     7561 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
     7621 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
     7681 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
     7741 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga
     7801 gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct
     7861 ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca
     7921 tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt
     7981 tcgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
     8041 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
     8101 ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt
     8161 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
     8221 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
     8281 cgctttcctg gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga
     8341 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
     8401 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
     8461 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
     8521 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
     8581 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
     8641 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
     8701 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
     8761 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
     8821 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
     8881 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
     8941 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
     9001 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
     9061 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
     9121 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
     9181 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
     9241 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
     9301 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
     9361 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
     9421 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
     9481 ggtaaccctg ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca
     9541 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg
     9601 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
     9661 gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca
     9721 gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat
     9781 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca
     9841 tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
     9901 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct
     9961 tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    10021 gcgtagcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    10081 tgtcgttgag cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    10141 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct
    10201 ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa
    10261 cgcccagttt cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta
    10321 ccagcgcggt ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    10381 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    10441 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    10501 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    10561 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    10621 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    10681 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    10741 aacccacaat gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg
    10801 tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag
    10861 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    10921 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    10981 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    11041 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    11101 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    11161 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    11221 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    11281 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    11341 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    11401 ggctgcgggt aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg
    11461 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    11521 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    11581 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    11641 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    11701 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    11761 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    11821 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    11881 tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt
    11941 ttgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    12001 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    12061 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc
    12121 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta
    12181 gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa
    12241 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt
    12301 gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag
    12361 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    12421 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    12481 accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    12541 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    12601 gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg
    12661 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca
    12721 cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc
    12781 tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat
    12841 gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc
    12901 ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac
    12961 ctgcctgcag tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat
    13021 tactacagcg ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa
    13081 aacatacaat tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg
    13141 ggaaaatgca cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13201 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13261 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg
    13321 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13381 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13441 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13501 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13561 tgtgccagct tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag
    13621 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13681 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13741 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13801 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13861 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    13921 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13981 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    14041 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    14101 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14161 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14221 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14281 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14341 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14401 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14461 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14521 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14581 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct
    14641 ggaggccttc tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14701 gccgcgcacc cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat
    14761 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc
    14821 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14881 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca
    14941 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15001 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15061 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15121 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15181 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15241 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15301 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15361 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15421 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15481 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15541 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15601 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15661 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15721 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15781 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15841 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    15901 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15961 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg
    16021 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    16081 ctgacgtggg caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc
    16141 gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    16201 ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16261 gcggcaacgg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16321 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg
    16381 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16441 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16501 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16561 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    16621 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    16681 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16741 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16801 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    16861 aagaggatga agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag
    16921 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    16981 tagcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    17041 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17101 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17161 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17221 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17281 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17341 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    17401 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17461 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat
    17521 ttgcttttgt taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg
    17581 caacgtcagt aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17641 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17701 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17761 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17821 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17881 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    17941 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    18001 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18061 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    18121 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    18181 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    18241 taataacggc tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    18301 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    18361 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    18421 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    18481 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    18541 tttgtaaatt ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa
    18601 cctataaatc aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat
    18661 aatattaatt tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc
    18721 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca
    18781 cggcgaccag gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa
    18841 cgtcgcttta ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc
    18901 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18961 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc
    19021 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    19081 ctgttcgcca cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga
    19141 ataccccagc aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc
    19201 ttcgcagact tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg
    19261 aatgccaata atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc
    19321 aaaaccggac gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19381 aatggtttcg aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag
    19441 tttggcgacg gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg
    19501 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc
    19561 agcatcggca tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt
    19621 ttcactgccc gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa
    19681 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19741 caccggtacc atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc
    19801 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19861 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19921 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19981 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    20041 acggtacatc cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc
    20101 ggggcgtccg agatagccct gcgccagttg aatgccggta agatagagat cacccgccac
    20161 acccggcggc accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg
    20221 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    20281 ccagctgaca tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt
    20341 taactgttgc cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg
    20401 tttcaacgtc gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc
    20461 cagcatcgac ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat
    20521 agcgagcggg tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa
    20581 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20641 atcttcgcct gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt
    20701 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20761 cgtgtggtgc ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt
    20821 atagcaaagg cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt
    20881 aattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20941 ggtatccagc ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa
    21001 aaagaccgag cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg
    21061 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    21121 cgcatctgcc agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct
    21181 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc
    21241 acctggcagc ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc
    21301 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt
    21361 attggcgagg atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag
    21421 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21481 ttggtaatca aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc
    21541 tcgcccgctg tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt
    21601 cagttgagcc gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat
    21661 acccaacggt aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg
    21721 acgcataaag ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa
    21781 ggctgccagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21841 gcggaattcc ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg
    21901 ccccggtaaa ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc
    21961 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    22021 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca
    22081 gtaaatattg gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg
    22141 gtgataacgc tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac
    22201 cagtggttta ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg
    22261 cgcagtaccg tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat
    22321 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa
    22381 cgtatctgct tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc
    22441 tccagttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22501 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22561 tgcaactggg tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg
    22621 caggacgcct gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt
    22681 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22741 tcatcgaagg gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga
    22801 ggtcgattaa cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22861 cctgacgcca gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg
    22921 cccgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc
    22981 taacttcacc agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg
    23041 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    23101 cggcatacag cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    23161 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    23221 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca
    23281 acacatacac ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga
    23341 cgggcatact ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg
    23401 ctgttacatc gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt
    23461 tccagataat ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca
    23521 gaggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23581 gtggattcag cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23641 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt
    23701 agctgccgcg ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa
    23761 ttcgctgcat cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga
    23821 tccatacgcg ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag
    23881 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23941 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    24001 ttcattgatt gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc
    24061 gtaatggcgc gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca
    24121 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt
    24181 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    24241 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24301 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc
    24361 gtttcgacca tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag
    24421 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24481 aataaccgac agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24541 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24601 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24661 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24721 aaaggcagcg gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa
    24781 gaggaaggtg ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc
    24841 agcttccgct tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag
    24901 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24961 gtgatcaaca aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg
    25021 gaactggaag caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc
    25081 aactccgatt cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag
    25141 aactactcgc tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg
    25201 cagtatgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg
    25261 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg
    25321 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg
    25381 gagtggaatc agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg
    25441 aacaccggcg gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25501 gaaattttct cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg
    25561 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac
    25621 atatcgcaag gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa
    25681 gcaccgagcc tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc
    25741 tatgccagcg cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc
    25801 atcaacaaag agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg
    25861 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25921 gtcggcaccg atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa
    25981 ggatcgttaa acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg
    26041 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg
    26101 aactcaacgc tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg
    26161 tacggtaagc agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa
    26221 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    26281 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26341 aatgcccaga ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat
    26401 acctataacg agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa
    26461 cgtcagattg ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc
    26521 ggcctacaaa aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta
    26581 gcgcatcagg cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg
    26641 catacctccc tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat
    26701 ttttgtgagc aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt
    26761 aaacgtaaaa cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat
    26821 ggctataaat gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta
    26881 tacggcagta ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt
    26941 ggcattgata tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt
    27001 attacaccag cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg
    27061 acactggcat tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca
    27121 ggttttctgg attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag
    27181 aatgagatgt ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa
    27241 cacgattaat tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa
    27301 agtgtaaagc aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc
    27361 cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt
    27421 cctttacggt gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca
    27481 aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat
    27541 gcgaagaagg gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata
    27601 gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
    27661 tcaggcaccc gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa
    27721 gtcctgtttc ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc
    27781 agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa
    27841 ccggatatgc ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    27901 agccttaaac tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag
    27961 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt
    28021 cagcatgttt atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg
    28081 gagtggcagc cccagggttg atagaaatat ctcctggggc ttcatctttc cagacctcag
    28141 agtaaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt cctcaacaaa
    28201 atggctgttt atttatacag taaatcctgt ttcggacttc tcactcctca tttaatcaac
    28261 tgaagctgag cccgtttttt ctgcatgcct caatcgtcaa aattcttcgc catcagccag
    28321 ctctgccata acttgccaca ttgaaaaagt agccaaattg cttcagtcac catcatcaaa
    28381 aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata
    28441 gaaaacgcca acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt
    28501 taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca
    28561 ggactcttca atgctgattg acgcggttaa aaataagatc acggccggag aggtaaagca
    28621 cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc
    28681 tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct
    28741 ggaaatttgc ggcggtggca cacacccgtt tcagaaatgg cagcgtcagg aggtatgcga
    28801 taacgagcgc tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt
    28861 ttttggtcag catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca
    28921 cggcttgtca cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca
    28981 aggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa
    29041 tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgtttgag
    29101 ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca
    29161 ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa
    29221 tatggcggga ttaattcagg ctaccgccca ctggttactg acagaacgcc cgttcaaaca
    29281 tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct
    29341 ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagacacctt
    29401 gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga gcgcgattga
    29461 ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt
    29521 cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccgatga
    29581 ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt
    29641 aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg
    29701 gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa
    29761 ctggtgacca tgttccatct gatggatctt agctagcttt acactttatt gttttgtctg
    29821 tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg
    29881 ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc
    29941 tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct
    30001 ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt
    30061 taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa
    30121 tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc
    30181 agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga
    30241 agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
    30301 acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc
    30361 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt
    30421 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
    30481 taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc
    30541 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
    30601 tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc
    30661 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
    30721 tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa
    30781 aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat
    30841 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg ctctgggtct
    30901 ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa
    30961 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
    31021 taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    31081 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    31141 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    31201 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    31261 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    31321 cggaccttcg aaaaacgagc catcgccagt tcacggcttt ggttgcaaat cataacccag
    31381 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    31441 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    31501 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    31561 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    31621 ctgatcggcg cgatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    31681 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt atttaaagat
    31741 ccgattcttg gtctggtggc cggtattcag ctttccgcga acgatatgct gaaactgggc
    31801 gactggctgg agatgccgaa atacggcgca gatggcgcgg tgatcgatat tgggttaacc
    31861 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
    31921 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    31981 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    32041 aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg
    32101 aatcgccagc aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga
    32161 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    32221 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
    32281 tttactaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    32341 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
    32401 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    32461 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
    32521 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    32581 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcag agatagtttc
    32641 cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac
    32701 gaaaacgcag atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca
    32761 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaac accaccagcg
    32821 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
    32881 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    32941 aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag
    33001 ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    33061 cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga
    33121 tgcgatacac cacctgattt accgcttttg gaatgctttt atccggatcg cgcgcttcag
    33181 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    33241 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    33301 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    33361 tgattgccag cactttaatc aacgcaaacc agaactcggt ttcgccatat aagcgcacgt
    33421 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    33481 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    33541 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    33601 tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc tcgccaagct
    33661 ggcgcataat caggaaagcg atgatcccgg cgacgccgta acccagcaat acagccggac
    33721 ccgccatctg aatcgccgga ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    33781 gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
    33841 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    33901 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    33961 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    34021 cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg
    34081 tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag
    34141 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagta ccgcgccgtg
    34201 atacaacgcc tcatccagct tctgctcact gaatgtttgc ggattattca acgacggctc
    34261 ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc
    34321 ggcaagggca ataaaaccgg tgcccgttgc cacagaaaga tgaaagccca tccaccacag
    34381 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    34441 cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca tcggcaccat
    34501 gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    34561 ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga
    34621 caccatgtca cgatcgcggg catcaatata aatccagctc gtcgggcgcg cattctcggt
    34681 tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca gggtgatttg
    34741 ctgcttcatc ggcgtcagga tcggtagctg gcgcagtgcc tgcgggctat cgcgccagct
    34801 ttgcggataa cgcagattaa tgggataacg ggcaatccct tccaccgttt cgccaaccat
    34861 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    34921 cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gacgctcggc
    34981 tagagcagaa gccacgcttg gcaccgttcg cgctacttct tcaatttgct cagccatcgc
    35041 gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt
    35101 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    35161 ccgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgccag gccgccactg
    35221 atcctgcggc ttaagctgga tggtcgtttc aaccatctcg agcggagcag aatcggtggc
    35281 ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    35341 cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga
    35401 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    35461 gagcggccag agaaccgtca gcaccgaaag cgctgccacc aacagcgtgg ttttcggcca
    35521 gtgcagtact ttcagcaata gaggatgata aacacgaatc aaaaagcgat tgagcgggtt
    35581 actgctttcc ggcggaattt tgccacggat ccagtacccc atcaggatag ggatcactac
    35641 gatcgccagc agcgccgcgc ccgccatcgc ataggtttta gtgaacgcca acgggccaaa
    35701 cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag
    35761 cagactgata aatagcgccg gacccacttc aacagatgca tcggtgatca cctgccagcg
    35821 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    35881 tgcattttcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    35941 gcccaacgac ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca
    36001 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    36061 cgcacagact accgccacca cgataaactc ttccagcaac ttgccgctga ggttgtcgat
    36121 ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    36181 actacttttc agcgtttcca gtttgtcctt cacggaggcg atcacttctc gggcgttttt
    36241 gccggagcgc aggatcacca ccccgcccgc cacttcgcct tcgccattca gttcggcaat
    36301 gccccggcgc atctccgggc caatctggac cttcgcaaca tcacgcagat aaacgggcac
    36361 gccattttct ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    36421 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    36481 gtttgaagca tccagcgcgc tttttacttc ggctagactg atgccatatt gcgccaggcg
    36541 ctggggatcg ataaccacct gatactcttt caccacgccg cccaccgacg ccacttccgc
    36601 aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg agcgtaaatc
    36661 ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    36721 cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
    36781 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt cgaaaatgac
    36841 atacacataa gagtctccaa actgcgagaa accgcgcaca gtcttcgcgc caggcaccga
    36901 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg gtgcctgacc
    36961 gggatagctg gttttaataa tcacctgcac atcggagaga tccggcagcg catccactgg
    37021 cgtgttaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
    37081 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    37141 gcattggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    37201 tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagtgtaac
    37261 gcggtgacgc cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa gcgtccgtcg
    37321 gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctg tgacggaatg
    37381 agcagcatcg gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc
    37441 gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg
    37501 ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg
    37561 agcgtaaact gcgaggcatc tttcaccagc caggcgatag actccgggat cgcagcagtg
    37621 acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt
    37681 cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gtgctttgag cgtaaagcga
    37741 gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt
    37801 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    37861 aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc
    37921 ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    37981 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    38041 aaagtcagcg gtccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    38101 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    38161 atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg
    38221 taccagaata agattttacg ttctgcggtc gacgtttttt ctgcgggtgg ttcagccttt
    38281 gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata
    38341 agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat
    38401 aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccggt tttaatttca
    38461 ctcattttcg tctgcggggt gatggtaaag cgcatagtca tctccggcca gttcacggca
    38521 gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttaccacg
    38581 ccagtggcgc taataacctg cggttgtact tcgctcatgg tttcatgatg atgttcgtta
    38641 gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt
    38701 ttcatttatt ctctcctgga gttaaaaatt attgctgcca accgccccct aaggcggtat
    38761 acaaagaaat ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg
    38821 caaataaaga acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata
    38881 atgcccgcgc ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga
    38941 tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct
    39001 ggattttctg ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat
    39061 tggcctggtt gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca
    39121 tcccgctgct agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg
    39181 tcaggctgat agacgggaaa aatgccgcac gcgcagcacc aatattagca ttagccgcca
    39241 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaagc
    39301 ccgccggtaa tttaacgctt tgcaggctgc cgctgtttac tgtctgcgct tgcggcagtt
    39361 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac
    39421 gtttagcgat gtcactgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    39481 cattgctgct accggtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    39541 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    39601 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    39661 aattttgtcg ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc
    39721 tggcgttaag gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc
    39781 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    39841 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccgg ttattcacca
    39901 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccacgt
    39961 tctggtagtt atctgctgcg ttaaccaagc cgttctggct gagtgagaac tgctgcggca
    40021 cgggcattgc cgtacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    40081 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatacc
    40141 gccaacttta ctcgccaggc tctgattttc cggtgacagg aaaatgacaa aattgtcatt
    40201 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg
    40261 aggaaaaatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    40321 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggctga atggctacca
    40381 tctggcgatg accggtgatt atgacctgat aatcctcgat attatgctgc cggacgtgaa
    40441 cggctgggat atcgtgcgca tgttgcgctc cgccaataaa gggatgccga ttctgttgct
    40501 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg ccgatgacta
    40561 tctggtgaag ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg
    40621 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt
    40681 cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct
    40741 gctggagttt tttctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    40801 ggtgtgggac atgaatttcg acagcgacac taacgccatt gatgtggcgg tgaagcggct
    40861 acgcggcaaa atcgacaacg actttgagcc gaagctgatt cagaccgtgc gcggcgtggg
    40921 ctatatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    40981 cccgtctgac cttttttatc agcctggcca ccatcgcggc gtttttcgcc tttgcatgga
    41041 tcatgatcca ctcagtgaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa
    41101 ttagcgccac ccttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa
    41161 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatttccctg gcagatagcc
    41221 acggcaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    41281 ccatacccga taaagacgct cagggtggcg aggtgtatct cctttccggc ccgacgatta
    41341 tgatgccagg ccacggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgc
    41401 cggttggccc gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    41461 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatctgtca
    41521 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    41581 gcagcgtcag ccgtcaaatc cagaatatca cctcgaaaga tctcgacgtt cgccttgacc
    41641 cgcagaccga gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    41701 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    41761 gcacgccgat taccaatctc attacgcaaa cggaaatcgc cctcagccag tctcgcagcc
    41821 agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    41881 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    41941 aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg
    42001 aagatcgcgg cgtggagttg cgatttgttg gcgacaaatg tcaggttgcg ggcgatccgc
    42061 tgatgctgcg tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccaacca
    42121 gagagacaat tgtagtgcgc tgtcagacgg tcgatcacca ggtgcaagtt accgtcgaaa
    42181 accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg
    42241 ttgacccttc ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat
    42301 cgattgttgt cgcgcataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt
    42361 ttgtgattat gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat
    42421 cacctcctgg tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc
    42481 gaaggctttt atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg
    42541 tgtttcttcc aattgtagat cacaatgccc tttctaaatt ttcatcgtta taaacaagcc
    42601 atctatcata taaagcaatg aataatttaa tcgcgattaa tcatcattaa ttgttatatt
    42661 attcactcaa tctatttgtc aagtgaattt gataaattcc taatttaaaa tatccactat
    42721 tgagccagta tttaacaacg ttaagatgtg caatgtgcag atcatgttat cagggtggag
    42781 cggtagcata tatttcttat gcaatcattc attttaataa tgttaattgg attatttatt
    42841 gctgtgaata aaaatatatt atgaatattg gtttaacttt gcgtttttgt tactcgttgt
    42901 gatatgcgta cctgtcctga ataacgccaa gaaacaagag gttgagcttg tgattatttt
    42961 tttgactgaa cagacacccg tttctgtaaa gtaatattta aacaaaattc tcacataacc
    43021 atagcatttt ctgttccttc cctaataacg tctgaagtcg tcccgcttat cacattatcc
    43081 gcagttgtac ggtacggatc attattcatt ttactggagg ttgctatgtc aaccggatta
    43141 cgtttcacac tggaagtgga cggcctgccg ccggacgctt ttgcggtggt ttcctttcat
    43201 ctgaaccagt cactctcttc gctcttttcc ctcgatctct ccctggtcag ccagcagttt
    43261 ctctcccttg aatttgcgca ggtgctggac aaaatggcct acctgacgat atggcagggc
    43321 gatgaagtac agcgccgggt gaaaggcgtg gtgacctggt ttgaactcgg ggagaacgac
    43381 aaaaaccaga tgctgtacag catgaaggtg cacccgccgc tgtggcgtgc cggtctgcgc
    43441 cagaacttcc gtatcttcca gaacgaggac atcaaaagca tcctcggcac gatgttgcag
    43501 gaaaacgggg tgaccgaatg gagtccgctg ttcagcgagc cgcatccttc ccgtgagttt
    43561 tgtgtccagt acggtgagac tgattacgat ttcctgtgcc ggatggcggc ggaggaaggc
    43621 atcttctttt atgaggagca tgcttacaaa agtaccgacc agagcctggt gctgtgcgac
    43681 acagtccgcc atctgcccga atcttttgaa atcccatgga acccgaacac ccgtaccgag
    43741 gtgagcaccc tctgcatcag ccagttccgc tacagcgcac aaatccgccc ttcttccgtg
    43801 gtgaccaaag actacacctt taaacgcccc ggctgggccg gacgttttga acaggaaggc
    43861 cagcaccagg actaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag
    43921 ggtgctcacg ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caatgcagaa
    43981 accgcgcggg gaatgagtcg ctcgccggag atatggccgg gacgacgaat tgtgctgacg
    44041 gggcatccgc aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc
    44101 gaacagccac aggcggtgcc aggacggcag ggagcgggga cggcgctgga gaaccatttt
    44161 gcggtgatcc cggcagacag aacatggcga ccacagccgt tgctgaaacc gctggtcgac
    44221 ggcccgcaga gcgctgtcgt gacaggaccg gcaggcgagg aaatcttctg cgacgaacat
    44281 ggtcgcgtgc gggtgaagtt caactgggac cgttataacc cggcagacca agacagttcg
    44341 tgctggatcc gtgtggcaca ggcgtgggca ggcaccggtt ttggccacct ggcgataccg
    44401 cgtgtgggtc aggaagtgat tgtagacttc ctcaacggcg atccggacca gccgatcatt
    44461 atggggcgca cctaccacca ggaaaaccgc ccccccggca gcctgccggg aacaaagacg
    44521 cagatgacca tccgctccaa aacgtacatg ggcagcggat ttaatgagct gaagtttgat
    44581 gatgcgacag ggagagaaca ggtctacatc cacgcgcaga agaacatgga taccgaagtg
    44641 ctcaacgacc gtaccaccac cgtaaaacac gatcaccgcg aaaccgtaaa aaatgaccag
    44701 acggtcacga tccaggaagg taaccgcctt cttacggtgg aaaaaggcca caagatcacc
    44761 ggagtactga aagggtcttt atctgaggat gtctttcagg acagaggcac gattgccggt
    44821 tcggtgcatg ttgacgctgt aaacaatggt ggcgaaggcg acggtataca ggcttatacg
    44881 gcgattaagg aaattttgct ggccgtggag gaaagcaaaa ttgcgctgac gccggatagc
    44941 attcagctac aggtcgggga atcgacggta atcaggctgt cgaaggatgg catcaccatc
    45001 gtgggcggtt ctgttttcat caactgattc actcagatta tcagggagta ttttatgcag
    45061 ttcactttta atgaaggcca cattcaactg ccgtctcaat ggcaggatca gtcaatgcag
    45121 gtcctggtgt ccacagataa cagtggcatc aacctggtca ttacccgcga agccgttcca
    45181 cagggaaccc tgacaccaga gctgtaccag gaaacgctgg cgctgtacca gggcaaactt
    45241 gatgggtaca ccgaacatgc ctgccgggaa atcactctgg cagaggcccc ggcctggctg
    45301 ctggattatt cctggaacgg tccggaagat gaggggaatc aggggcggat tagccagata
    45361 gccgtattcc agcggcgtgg agatacgctg ctgaccttca ctttcagcac ctccttatcc
    45421 ctgaagaaca gtcaaaagac gatgctgctg gaggtgataa agagcttcac cccgttgcca
    45481 ccggagaatg acatccaaaa ggaccagccg cgctgacata aacgagggat aacacatgag
    45541 tgaaggacca ggcgggccac agggagcgac cgcaggcggt acgctggcaa tgcgaatgct
    45601 gtcacagcag gcgatggtcg ccagccagat gaaacgggca gccaacgaca aagccattgc
    45661 acagatgttg gcatcaaaaa agtctggccc ccccgccgcc aggctgggcg atgaaattca
    45721 gcacaagagt tttttggggg cgctggcagg ggccgtactg ggggcgatag tgaccatcgc
    45781 agaaggttgc ctgattatgg ccgcctgtgc caccggccct tatgcgctgg ttctggtacc
    45841 tgcgctgatg tatgccagct ataaggcgag tgattatgtg gaggagaaac agaaccagct
    45901 tgaatcatgg ataaacagct tttgtgacac ggacggcgcc atcaataccg gttctgaaaa
    45961 cgtaaaaatt aacggcgagc tggccgcacg tgcagcggtc acccttcccc ctcctccccc
    46021 acctggagca atacctgaag tcccacaggg ggaaccctca tggggtgata ttgccactga
    46081 cctgcttgaa tcggcagcgg aaaaagcagt accactggcg aaggcctggg ggaacgctgt
    46141 tatcaccctg acggacagca atgccggttt tatggatcgc gtcagcgccg gcgcatcgct
    46201 tctgtttccc gccggtccgg tattaatgga gtttgccacc atggtgggcg ggcgtggcga
    46261 aatcaaaaaa gatgtggatt tcccggaagc cggtgaggac acggcgctct gcgacaagga
    46321 gaacaaacca ccgaggatag cccagggcag cagcaacgtc tttatcaaca atcagcctgc
    46381 cgcgcgcaag ggcgacaaac tggagtgcag tgcggcgatc gtgggaggtt cgccggacgt
    46441 ctttattggc ggtgagcagg tcacatatct ggatatccag ccggagttcc cgccgtggca
    46501 gagaatgatc ctggggggaa taacgatagc cagctatctt ctgccgccag cgggactact
    46561 gggaaaactg aagaatctgg cgagactcgg caaactggga aactgctggg gaaaagcggg
    46621 aagctgctgg gcgcaaagct cggcgcgttg ctggggaaaa caggtaagtc gttaaaaagt
    46681 attgccaata aagtcatcag atgggtaaca gatcctgtcg atccggtaac cggcgcatac
    46741 tgcgacgaac gtaccgactt caccctgggc cagaccctcc ccctctcctt cacccgtttc
    46801 cacagttctg tactgccgct gcatggcctg accggcgtgg gctggagcga ctcctggagc
    46861 gaatacgcct gggtgcgtga acagggaaac cgggtggata tcatcagcca gggagccacg
    46921 ctgagatttg ccttcgacgg tgacagtgat acggcggtta acccgtatca cgcccagtac
    46981 attctgcgcc gccgcgatga ttatctggag ctgttcgaca gggatgcact gagcagccgc
    47041 ttcttttatg acgcctttcc gggaatgcgt ctgcgccacc cggtgactga cgataccagc
    47101 gatgaccgcc tggcacacag ccccaatgac cggatgtaca tgctgggcgg gatgagcgac
    47161 accgccagca accgcatcac gtttgagcgc gacagccagt accggatcac gggtgtcagt
    47221 cacaccgacg ggatccggct taaactgacg taccacgcca gcggctacct gaaagccatt
    47281 caccgcacgg ataacgacat acagacgctg gcgacctacg aacaggatgc gcgggggcgg
    47341 ctgacagaag cggatgcgcg gctggactac cacctgtttt atgagtacga cgctgcggac
    47401 cggatcatcc gctggtccga taacgaccag acgtggagcc gtttcaccta cgatgaacag
    47461 ggccggtgcg tgaatgtcac cggggcggag ggctattaca acgccacgct ggactatggt
    47521 gacggctgca ccaccgtgac ggacggcaag ggcattcacc gttattacta tgatcctgac
    47581 ggcaatattc tgcgggaaga agcgccggac ggcagcacca ccatgtatga atgggatgaa
    47641 ttccatcacc tgctggcccg ccactcccct gccgggcggg tggagaaatt tgaatacaac
    47701 gccgcacacg gtcagttaag ccgttacacg gcggcagacg gcgcggagtg gcagtaccgc
    47761 tatgatgagc gcggcctgct cagcaacatc accgaccctg ccgggcagac gtggacacag
    47821 cagtgtgatg aacgcggcct gccggtaagt ctggtgtcgc cacagggcga agagacccgg
    47881 ctggcataca ccgctcaggg gctgctgtcg ggaatattcc gccaggatga acggcgtctg
    47941 ggcatagagt acgaccacca caaccggccg gaaacactca ccgacgtgat gggccgtgaa
    48001 caccacaccg aatacagcgg tcacgacctg ccagtgaaga tgcgcggccc cggcggtcag
    48061 tcagtgcggt tacagtggca gcagcaccat aaactgagcg gcattgaacg tgctggaacc
    48121 ggcgcggaag gatttcgcta tgaccgccac ggcaacctgc tggcctggac ggacggcaac
    48181 ggtgttgtct ggacgatgga atacggtccg ttcgattttc cggtggcgcg aacggacggt
    48241 gaaggccacc gctggcagta ccgctacgat aaagacacgc tgcaactgac agaagtcatc
    48301 aacccgcagg gtgagtcata tctttatgtt ctggataact gcggccgggt gacggaagag
    48361 cgtgactggg gcggcgtggt ctggcgttac cgctatgacg ccgatggcct ctgtaccgcc
    48421 aaggttaacg gcctggagga aaccatcctc tacagccggg acgccgcagg ccgcctggca
    48481 gaaatcatct cgccggaagg caaaacgcag tatgcctatg acaaatccgg caggctgacg
    48541 ggtatcttca gcccggacgg cacatcacag cgcaccggct atgacgaacg cgggcgggtg
    48601 aatgtcacca ctcagggccg acgggccatt gaatacaact accccgatga acacaccgtc
    48661 atccgctgta tcctgccacc ggaagatgaa cgcgacagac accccggtga atccctgctg
    48721 aaaaccacgt accgctacaa cgccgccgga gaactgacag aggtcattct gccgggggat
    48781 gagacgctga cgttcagccg tgatgaggcg ggacgtgaag tgctccggca cagtaaccgg
    48841 ggttttgcct gtgaacaggg ctggaatgca gccggtcagc ctgtcagtca gcgcgccgga
    48901 ttttttccgg cagaagccac atgggacggg ctggttccct cactggtacg ggagtaccgt
    48961 tacgacagcg cgggtaacgt gtcaggcgtc accagccggg aagattacgg acgggaaaca
    49021 cggcgggagt atcggctgga ccggaacggc caggtcacgg cggtgacagc ctcaggcacc
    49081 gggctgggct atggcgaagg cgatgagtcc tatggctatg acagctgcgg ctacctgaag
    49141 gcgcagtctg cgggcaggca ccggataagt gaagagactg accagtatgc cggaggccac
    49201 cggctgaaac aggccggaaa cacgcagtat gactatgacg ccgcaggccg gatggtcagc
    49261 cgcaccagac accgtgacgg ctaccgaccg gaaacagagc ggttccggtg ggacagccgg
    49321 gaccagctga ccgggtattg cagcgcacag ggtgagctgt gggaataccg ccacgacgcc
    49381 agcggcagac gaacagagaa acgctgcgac cggaagaaaa tccgcttcac gtatctgtgg
    49441 gacggcgaca gtattgcgga aatccgggaa taccgcgatg ataaactgta cagcgtaagg
    49501 cacctggtgt ttaacggctt tgagctgata agccagcagt tcagccgggt acggcaggcg
    49561 catccgtcgg tggccccgca gtgggtgacg cggacgaatc atgcggtgag cgacctgacg
    49621 ggccgcccgc tgatgctctt taacagtgaa ggaaagacgg tctggcgacc ggggcagacc
    49681 agcctgtggg ggctggcact cagcctgccc gcagacaccg gctacccgga cccgcgcggg
    49741 gaactggacc cggaagccga ccccggcctg ctgtatgcag gacagtggca ggatgcggaa
    49801 tccgggctgt gctataatcg gttccggtat tacgagccgg aaaccggaat gtacctggtg
    49861 agtgatccgc tggggttgca gggcggggag cagacttacc ggtatgtgcc gaatccttgt
    49921 gggtgggttg atccgctggg attggctgca agttctaaaa tcagcagttt gatggactat
    49981 attggcgatg gtcgtcgtgt tagtgggcat acgggtttcc tggatggggt tcgtttatca
    50041 cgtagtcaaa taa
//
LOCUS       DAJTVK010000015        49830 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000015 DAJTVK010000000
VERSION     DAJTVK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.015"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..455
                     /locus_tag="OO372_000921"
     CDS             <1..455
                     /locus_tag="OO372_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6340497.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
     gene            complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO372_000922"
     CDS             complement(504..2003)
                     /gene="pitB"
                     /locus_tag="OO372_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6340498.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO372_000923"
     CDS             complement(2295..4154)
                     /gene="gss"
                     /locus_tag="OO372_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6340499.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4359..5225
                     /gene="yghU"
                     /locus_tag="OO372_000924"
     CDS             4359..5225
                     /gene="yghU"
                     /locus_tag="OO372_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6340500.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO372_000925"
     CDS             complement(5348..5596)
                     /gene="hybG"
                     /locus_tag="OO372_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6340501.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO372_000926"
     CDS             complement(5609..5950)
                     /gene="hypA"
                     /locus_tag="OO372_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6340502.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO372_000927"
     CDS             complement(5943..6431)
                     /gene="hybE"
                     /locus_tag="OO372_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6340503.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6424..6918)
                     /locus_tag="OO372_000928"
     CDS             complement(6424..6918)
                     /locus_tag="OO372_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6340504.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO372_000929"
     CDS             complement(6918..8621)
                     /gene="hybC"
                     /locus_tag="OO372_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6340505.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO372_000930"
     CDS             complement(8618..9796)
                     /gene="hybB"
                     /locus_tag="OO372_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6340506.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO372_000931"
     CDS             complement(9786..10772)
                     /gene="hybA"
                     /locus_tag="OO372_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6340507.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO372_000932"
     CDS             complement(10775..11893)
                     /gene="hybO"
                     /locus_tag="OO372_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6340508.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12082..12369)
                     /locus_tag="OO372_000933"
     CDS             complement(12082..12369)
                     /locus_tag="OO372_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6340509.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12488..13375)
                     /locus_tag="OO372_000934"
     CDS             complement(12488..13375)
                     /locus_tag="OO372_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6340510.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13532..14572
                     /gene="gpr"
                     /locus_tag="OO372_000935"
     CDS             13532..14572
                     /gene="gpr"
                     /locus_tag="OO372_000935"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6340511.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14612..15106)
                     /locus_tag="OO372_000936"
     CDS             complement(14612..15106)
                     /locus_tag="OO372_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6340512.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15297..16181
                     /gene="yghA"
                     /locus_tag="OO372_000937"
     CDS             15297..16181
                     /gene="yghA"
                     /locus_tag="OO372_000937"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6340513.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO372_000938"
     CDS             complement(16341..16766)
                     /gene="exbD"
                     /locus_tag="OO372_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6340514.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO372_000939"
     CDS             complement(16773..17507)
                     /gene="exbB"
                     /locus_tag="OO372_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6340515.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            17759..18946
                     /gene="metC"
                     /locus_tag="OO372_000940"
     CDS             17759..18946
                     /gene="metC"
                     /locus_tag="OO372_000940"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6340516.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19086..19745
                     /locus_tag="OO372_000941"
     CDS             19086..19745
                     /locus_tag="OO372_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6340517.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO372_000942"
     CDS             complement(19785..20684)
                     /gene="yqhC"
                     /locus_tag="OO372_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6340518.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            20878..22041
                     /gene="yqhD"
                     /locus_tag="OO372_000943"
     CDS             20878..22041
                     /gene="yqhD"
                     /locus_tag="OO372_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6340519.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22146..22973
                     /gene="dkgA"
                     /locus_tag="OO372_000944"
     CDS             22146..22973
                     /gene="dkgA"
                     /locus_tag="OO372_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6340520.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23173..24099
                     /locus_tag="OO372_000945"
     CDS             23173..24099
                     /locus_tag="OO372_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6340521.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            24150..24407
                     /gene="yqhH"
                     /locus_tag="OO372_000946"
     CDS             24150..24407
                     /gene="yqhH"
                     /locus_tag="OO372_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6340522.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24450..26669)
                     /locus_tag="OO372_000947"
     CDS             complement(24450..26669)
                     /locus_tag="OO372_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6340523.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO372_000948"
     CDS             complement(26780..28192)
                     /gene="ftsP"
                     /locus_tag="OO372_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6340524.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO372_000949"
                     /gene_synonym="parF"
     CDS             complement(28267..29004)
                     /gene="plsC"
                     /locus_tag="OO372_000949"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6340525.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO372_000950"
     CDS             complement(29237..31495)
                     /gene="parC"
                     /locus_tag="OO372_000950"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6340526.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(32041..32523)
                     /locus_tag="OO372_000951"
     CDS             complement(32041..32523)
                     /locus_tag="OO372_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6340527.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO372_000952"
     CDS             complement(32576..32968)
                     /gene="ygiW"
                     /locus_tag="OO372_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6340528.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            33120..33779
                     /gene="qseB"
                     /locus_tag="OO372_000953"
     CDS             33120..33779
                     /gene="qseB"
                     /locus_tag="OO372_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6340529.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            33776..35125
                     /gene="qseC"
                     /locus_tag="OO372_000954"
     CDS             33776..35125
                     /gene="qseC"
                     /locus_tag="OO372_000954"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6340530.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            35235..35816
                     /gene="mdaB"
                     /locus_tag="OO372_000955"
     CDS             35235..35816
                     /gene="mdaB"
                     /locus_tag="OO372_000955"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6340531.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            35847..36161
                     /locus_tag="OO372_000956"
     CDS             35847..36161
                     /locus_tag="OO372_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6340532.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO372_000957"
     CDS             complement(36209..38101)
                     /gene="parE"
                     /locus_tag="OO372_000957"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6340533.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO372_000958"
     CDS             complement(38130..38711)
                     /gene="yqiA"
                     /locus_tag="OO372_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6340534.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO372_000959"
     CDS             complement(38711..39538)
                     /gene="cpdA"
                     /locus_tag="OO372_000959"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6340535.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(39563..39985)
                     /locus_tag="OO372_000960"
     CDS             complement(39563..39985)
                     /locus_tag="OO372_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6340536.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO372_000961"
     CDS             complement(39986..40615)
                     /gene="nudF"
                     /locus_tag="OO372_000961"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6340537.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            40820..42301
                     /gene="tolC"
                     /locus_tag="OO372_000962"
     CDS             40820..42301
                     /gene="tolC"
                     /locus_tag="OO372_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6340538.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            42449..43120
                     /locus_tag="OO372_000963"
     CDS             42449..43120
                     /locus_tag="OO372_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6340539.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            43126..44286
                     /locus_tag="OO372_000964"
     CDS             43126..44286
                     /locus_tag="OO372_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6340540.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO372_000965"
     CDS             complement(44324..45112)
                     /gene="ygiD"
                     /locus_tag="OO372_000965"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6340541.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            45255..46028
                     /gene="zupT"
                     /locus_tag="OO372_000966"
     CDS             45255..46028
                     /gene="zupT"
                     /locus_tag="OO372_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6340542.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(46086..46256)
                     /locus_tag="OO372_000967"
     CDS             complement(46086..46256)
                     /locus_tag="OO372_000967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340543.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO372_000968"
     CDS             complement(46517..47170)
                     /gene="ribB"
                     /locus_tag="OO372_000968"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6340544.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(47274..47422)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            47544..47834
                     /gene="ubiK"
                     /locus_tag="OO372_000969"
     CDS             47544..47834
                     /gene="ubiK"
                     /locus_tag="OO372_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6340545.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            48117..48668
                     /locus_tag="OO372_000970"
     CDS             48117..48668
                     /locus_tag="OO372_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR6340546.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            48728..49830
                     /locus_tag="OO372_000971"
                     /pseudo
     CDS             48728..49830
                     /locus_tag="OO372_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT        12496 a        13205 c        12357 g        11772 t
ORIGIN      
        1 cagttccggc gctggtaatt acagcgtttg tcgcccgtcg cttactgcgc tttcgtcata
       61 tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga ccgttatcct caggaccaaa
      121 ttcctggcgc ttacgatggt acggtgttcc cacctaacgt tgaaggtggt cgttttgtct
      181 catggctggc aagccaggaa cgtaaagcgt ttcactggat ggcgagccat aagcctgatc
      241 tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc acgtaaaccc gaccataaac
      301 gggaatcgct ggcgaggaag attgccataa cgccacagtt aacctttggt ggtgcacaac
      361 tggttgatat cgatgccaat cagccactgg aacaggtgtt ggttgatgca gaaaaagcga
      421 ttacggattt tatgaccgcg cgtggttatc actagtcaaa aatggaaatg cccgatcgcc
      481 aggaccgggc attttcagga aggttaaatc aactgcaatg ctatccagta cagcccacca
      541 gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca tcaggatgct ggttaccgtt
      601 ttacgctgta acccaccgcc gtccaccacc atcgtccctg ctaccgcaga cgagaggacg
      661 tgtgttgtgg agacaggcat cccaatataa ctggcaaggc cgatagacac tgccgccgtc
      721 atttgtgccg ccatgccttg cgcatacgtc atgccgcgct taccaatctt ctcaccgatg
      781 gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc cgagcgccag tgctaccgcc
      841 atgatgatcc acaccggagc gtactcaatg gtgcttaaca tatcgctgcg cagttttttc
      901 agcaggttct ggtcttcttt actgacgcct ggcagtttcg ccagcttcgc ggaggtatca
      961 gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac tcacgcttaa cggctcgtaa
     1021 ctttccatat tgcctggcag catcgtttta acgcgcgcaa tcgcatcaaa ggtatttgcc
     1081 ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg atgctgcagg caatggaggt
     1141 tccatcgcaa tcaacttctg cggcagttca ggatgctgtt gcaggtagtg ttcgaagttg
     1201 gtgacggcat cgcgggtacg ggtaatttca tagccggacg cgttcatatt gacgacgaag
     1261 ccagcagggg caatccccac cagtaccagc atcaccaggc cgatcccttt ttgtccgtcg
     1321 ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa tcagcgcaat acgcgtccag
     1381 aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt ccggaatgcg gtgaatacgg
     1441 tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga atatcaggcc tcccgcaatg
     1501 accaggccga cgatagggga aacaatcagc gaggagaaaa ttttggtcac ttcacgcagg
     1561 tttaacgcat ccatcaccga tgagccggtt aacagcgcgt tggttaaacc gatgccgata
     1621 atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac cgaagaacca cgttcccagg
     1681 ttccagataa tcgccgccag cagcatggaa aagaccatcg ccaggccgtg ggttgacccc
     1741 atattcagca acaaatcggt tggcaacata tggacaatgg cataggcaac gctaagtccg
     1801 cccaataaca cgccaaaaaa gttaaaaaat gccgccatca ccacagcaag ttgtggttgc
     1861 atggcacgag tataaataac ggttgccacc gcattcgccg tgtcatgaaa accattgatt
     1921 gcttcgtaga acaacacaaa tgccagagca agcaataaca aaagccctgt gtatatatca
     1981 aggccaacaa ataaatttag catatattag attacgccat tttgaattta cgaacggacg
     2041 cattatcagt gactttaacg gcatgggcaa agtgaaatat cattttttaa cgataaaaag
     2101 agtgttattt cttgtgtatc agacaataag cattcattca aaacctttag aaataatcaa
     2161 aacacaggta ttccactggt taatatattc acatatgaaa taaataaata ttagaacata
     2221 taaatattga atattttgat taatccccgt actgattatt cttcataatc agtacggggt
     2281 tgctacaaca tgtattactt tttcaccaca attaacggtt caatatcact ctcttttttg
     2341 atgaccagtg attcatcacc gcgcaaacac gtcccaccgt agttgccgcc cacggtgaag
     2401 gtacatacct gaatgtattt accgtccact ttcggcaaac accacagttg ctgatagatg
     2461 tttttctgct cggcaaattt accgctggtt ttgtccagca cctcttcatg atggctgacc
     2521 aggtcgatat tgctaccaca gcgaccggca atcggtttca ctgcgtaacc tgttttcacc
     2581 aattcatcat taacagtgaa atcggtatcc agcaggtaac gatggtgcgg gaacagcgac
     2641 cagaggatcg gcagaatcgc tttgttgccg gggatcaccg tccacagcgg ctcaaagact
     2701 agcacttccg ggcgcagcaa tacgtcgata agacgcactt cgttttgcgg atgaccggta
     2761 cggattggca ccgcagcaaa ctcacggtcg ctaacttcac gaatctgatc aaacgcggtt
     2821 tcccacgccc aggttttcca cacgcagtta accagtcgcc cttccccatc aatcagttgc
     2881 ccggcagcat cccagcccag ttcatccaat ccacgcaaga tacgcgtttc aaagcccgcc
     2941 tggtgcagcg cctgctccat aaactgcgcg tgatagtttt cctcgatatc tttgtcctgc
     3001 atgatatgga caaacggacg tgcacgactg tgtttccagg caccagccaa ttcgttaatc
     3061 agcccttccg ccggattgaa gccgttgcct ttatagccct gctccgccca acgttcgagg
     3121 atcaagcccg cttcggtatg acaggaggcg gagtcggcgt tgtactcgta aaccttcagg
     3181 ccacgctcat ccatgcagaa atccatacga ccagtgatca tatggtgacg gcgacgctgc
     3241 caggagagac gcaaacgtgg ccagaggatt ttcgggatgt cgaacagcgc cagcaggttg
     3301 tcatctttca gcaccttgtc ggttgcgtga agatacatca ggtgcagctc gttggtggct
     3361 ttaattagct cctgctcggc actctcggta atggtgtagt aatgataagg atcctgattg
     3421 atcacctgac cgttggcctg cacataggcg ttttgcagcg gatctttttc atccagccat
     3481 ttaccgtcaa actggccttt gttttccagg cgcgctccgc tgattttcag cagctcgcct
     3541 gcaatttccg gctgcggtaa gctgtattca gtatcttccg tctggatcat ccagcccaga
     3601 atggtggtgt catcaaaagt gtctttcagg gtatagcagc cgttttcgac caccatctcc
     3661 agctcacgcg tccactgttg cccttgcggc aacggggaat gaatcacgtt ctgttccgca
     3721 atacggactt tgttgccatg caattgggta atgatggcga catggccagt gtctttaaat
     3781 tcaccgcctt tatcccagat aagaagcgca cccgcgaccg gcgcacgcgg cgagccgtta
     3841 ggaaatgcct gcaatggcag gatgttgtca ttaaccactt ctcgcaggaa gcgcagcgag
     3901 aaaatctccc acgccatacc cacgtcagta aagaccacac cgtaattcag aaagagaaaa
     3961 cggcgagcaa attcaacgca ttgccacttg tggcccatat attcgtcgtc gatatagcta
     4021 cggaatacgg cgtcatcttc gtattcctgc ggatcgagag aactgtaatc tgaagagtag
     4081 attgctaccc cacctggggc gtagcccaat aatgtcccga acggggcatc ctggctggtc
     4141 gttcctttgc tcatcacttt acctttaaca atacaaccta agcagttggc gtaattgttg
     4201 tgctctgatt acctgctcag cgacattaac cggacagagg tcagactaat catacacctc
     4261 atcgcagcgg ctggcgcaaa gggttaaaaa attcacattc tgtacagcaa gtgacctgct
     4321 acactgcttc aacactacca ctcagaaggc aactcactat gacagacaat acttatcagc
     4381 ccgcgaaagt ctggacgtgg gataaatccg ctggcggcgc gttcgccaat atcaatcgcc
     4441 cggtttctgg tccgacgcat gaaaaaacgc tgcccgttgg caaacaccca ttgcaacttt
     4501 attcgctggg aacgccgaac ggtcagaaag taacgattat gcttgaggag ctgctggcgc
     4561 tgggcgttac tggtgcagag tacgacgcct ggctgattcg tattggcgat ggcgatcaat
     4621 tctccagcgg ctttgtcgaa gtgaacccaa actcgaagat cccggcgctg cgcgatcata
     4681 cgcataatcc gccgatccgc gtgtttgaat ctggttcgat cctgctttat ctggcggaga
     4741 aatttggcta cttcctgccg caggatttgg caaagcgtac tgaaacgatg aactggctgt
     4801 tctggttaca gggcgcggca ccgttcctcg gcggtggttt tggtcacttt taccattacg
     4861 cgccgatgaa aattgagtac gccatcaacc gctttaccat ggaagccaaa cgtctgctcg
     4921 acgtgctgga taagcaactg gcgcagcata agtttgttgc gggcgatgag tacaccattg
     4981 cggatatggc gatttggccg tggtttggca atgtggtgtt aggcggcgtg tatgatgccg
     5041 cagagtttct tgatgcgggc agttataagc atgtacaacg ctgggcgaaa gaagtaggcg
     5101 aacgtccggc ggtgaaacgt gggcgtattg ttaaccgcac caacggaccg ctgaatgagc
     5161 agttgcatga gcgccatgac gccagtgatt tcgagacgaa tacggaagat aagcgtcagg
     5221 ggtaagggtt ggtgttcgtc gcagcaagcc atccaggccg gataaggcgt tcacgccgca
     5281 tccggcaatc gtgcacaatg cctgatgcga cgctgccgcg tcttatcagg cctacgaaag
     5341 ctaatcatca cgcgctggta atgtcgtaat ccatttggcg cagtgcgtct aatgtggctt
     5401 tggcttcatc ttcgtcgatg atgctcatgg caaatccgac gtgtaccagc acccactggc
     5461 ccagtagatc ggcagggtta ccttcacaaa tcagggcgat attcacgtcg cgcttgatac
     5521 cacatacttc aacctgcgca agctggtgaa tatcttcacc gacagccagc acctggcctg
     5581 gaacgccaat acacattatt aactccggtt attcaacttc aatacttttg acgatcagcg
     5641 aatcgccggt atcgacacgc aaccgctcgc cgtgacagag cggacactgc gcatcgtgct
     5701 gatgaatctc caccacctgg ctgcaatccc agcaccaagc ctgggcgggt ttatagacga
     5761 tatgtaaatc gcacccttgc gccaccgttc cgtggcagac aatttcaaaa ctaaaacgga
     5821 cggcgctctc ctcaacgcag gagagcgcgc caatttccag ccacacggcg gtgacgcgct
     5881 taacatcgtg ctgctccgcc tgccgttgga taatttcaac ggcgctctgg caaagagaca
     5941 actcatgcat tttcgccact cctgcgacca aacagcaggg cgcgacgccc tgcgtgtggt
     6001 acatccggat tcgtgactgg cagcgaaagg atcattcgtg cgcagtcatc cgtcaggcgt
     6061 tggccctctt caatcgacat gctgtgcgaa agcggcgaca tcagcgaaca ggagagatat
     6121 tgcgaaacac cgtccagttc gccaacggta aaggtcatag tgccatacgg cagttgcaga
     6181 ccaatttttt cactcacttt gcgcagcggc cagagttgat ccgggccggg gaagataact
     6241 gcactcagca tccacggggt gatcacacac cccgtccact gaccttcgaa cagcgtaaaa
     6301 tcagaaacat acaccggcat tgaaggatgc agaaaagaga gatcgtgcat cgaacgccgg
     6361 gcaatttctt caaacgctgc ctgtacttgc gccttcgggg aggtctggaa acctgctatc
     6421 tcttcagtca tgaatcgcct cccgtgggat ggcttccacg cctgattcgc gcagcgcagc
     6481 cagaacctgc tcaagcgcag gttcaatcat tgcttcaacc gtcggcgtta agccgatgtg
     6541 tggctccagc gattccggga tcacgccgac cagggtcagc tttttcggaa actcaccggt
     6601 gaagcgcagg gccgacaata cgtcggccag gccaagctga tgcggagaga ttttgttggt
     6661 aaacaacgcc ggaacttctt catcccgcag gatcatcatc gttcccggcg tgttcttttt
     6721 cgacacaatg gcatccgcaa taatcagatg atcgcgattt gccatgtcgc caagcagctc
     6781 cattcccgcc gtgccgccat cgaggatctc aacataatcc ggcagaatgt atcgttgctc
     6841 taacgcttcg acaatccgca caccgatggc ttcatcggtc agcaaaatat tgccgacccc
     6901 taagactaaa atacgcatta cagaaccttc actgaaacca cttcgttgcc gtcggcatcc
     6961 actacgtgta ccgcacaggc catgcacggg tcgaaggagt gaatggtacg caccacttcc
     7021 agcggtttat tcggatcagc aaccggtgta cccaccagcg actgctcgta aggaccgacg
     7081 tcatcattga agttacgcgg accagagttc caggttgatg gaacaactgc ctggtagtta
     7141 ctgataatac cgtctttaat aaccatccag tgagagagca taccgcgcgg cgcttcgagg
     7201 aagccaacac ctttgaactc acccgttgcc ggaatgttcg gtttcacaaa ggtggtgtga
     7261 tcgcctttgc cgatattggt gatcagtgca ctgtattgat tttgcaggat atcctgcaat
     7321 tcgcagcagt gaacggtacg accaataata cggcccagcg tggagtgcag ctgcgccact
     7381 tccagcgtgt tgccagtcag tttctgataa atcgcaacga tttcattcag tttgttctgg
     7441 gtagattcgc gacctgccgc cagtttcacc agcatattag ccagcggacc cacttctacc
     7501 gttttgccgt agaaagtcgg tgatttcacc caggaatatt tcccgtcgtc agaccaacca
     7561 tcataagccg gaatggtggt accttcccac ggtgcctgcg gcgcttcgtc tttataccag
     7621 gagtgcttcg cgctttcctg aatgcctttg atcaggtatt catcggaatg agaagtgatc
     7681 ggacgatacg aggacagatc cgcattctca atgtagccgc ccgggaacag gaagctgccg
     7741 tttttactgt cggtcgggaa ttccggcacg ctcaggtagt tcaccgcacc tttaccgcgc
     7801 gtcagccatt ccgggtagaa cgcggcaata actgcggtat caaccttata aacctgttca
     7861 acaaagtcgc tcagcttgtc gatgaaagac ttgatgtaca tcaggcgctc aaggttcagc
     7921 acgcccaaac cgtcgaggtt gatcgggttc gcgacaccac ctaccgccag gttctgaatg
     7981 tgcggcgttt taccgcccag cagcgccacg acgcggttag cgtcacgctg gcactccagc
     8041 gcctgcaggt agtgcgctac cgcaatcagg ttcacttccg gcggcagttt catcgccggg
     8101 tgaccccagt agccattggc gaaaataccc aactgaccgc tggcaaccag atctttgatc
     8161 ttgttctgaa ctttagtgaa ctcttccgga ctgttcagat gccaggtcga aacgcctttc
     8221 agcatttcgg aggctttggt tgggtcagct tgcagtgcag aagtgatgtc cacccagtcc
     8281 agcgccgaaa gctgatagaa atgaacgata tggtcatgcg tggtgtgcgc agccagaatg
     8341 atgttacgga tgtattgcgc gttaaccgga acgtcgatat tcagcgcact ttctgccgca
     8401 cgaacggaag acagcgcgtg agtggtagta catacgccac agatacgttg cacaatcatc
     8461 catgcatcgc gcggatcgcg gtttttcacg atctcttcca tgccgcgcca catggtaccg
     8521 gaagcccatg ctttcgaaac gacgccattt tcgatttcgc aatcgatgcg taaatgcccc
     8581 tcaatacggg ttaccggatc aatagtaatt ctctggctca tgctttgctc gcctcatgac
     8641 gattatgatc gttttgtttt aaaggaggaa gtatcggcag tagacgaatg agtacgatgt
     8701 aagcgcaaat ctcaatagcc acaaaaccaa tagaaatcaa cagttcttcc caggtcggga
     8761 agtaggcgta accgccgccc gggttgaatg ccaccagcga ataggtcaga cgccaggttg
     8821 cacaacctaa cagtgcgctc agtgctgaca ggaacagcat gcgggaatca ttacgcagct
     8881 tcgccacacg cagaacgacc agcgggaaga gcatcagcag gacttcaatc cagaacatca
     8941 cggagtagaa gtcaccggca aacgctaacg acagcttgtc gcgatagata agctcgccaa
     9001 agcgcagcac gatgaaaatc gccagcaaca cactgatggt gttggtcagc ttgacgaaca
     9061 gactcttttc atccggaccg ttgccacgca gacccgcctg caccagcgaa ccttcaaaga
     9121 tgacaatcga gaagcccatg atgaacgccg tcagcagcga gaacagcggc aacatttcat
     9181 agctctgcca caacggatgc accttgtagc ccgccgagat catcagtgac cccattgaag
     9241 actggtgcat ggtcggcaac agcgcgccaa gtgcaatgat gaagaacata accttgttca
     9301 gacgcttgag agaaaccttc cagcccagac gttcaaacag tgccggagca aactccagtg
     9361 ccatcacgcc gatatagatg gtcatacaga ccgccgtctc gaacagtacc gagttcacgt
     9421 tgaagtgacc cggaatgtag aagtacggca ggttccagta gcgacccacg tcgatagtga
     9481 tcgacaagcc acccagtgag taaccaaaca gactcgccaa cagcgccgga cgcaccagcg
     9541 gatggtattg cccacggtta aagacgtata ccgcccacgc cagcgcccag ccgccacagg
     9601 caaagccggt gccaatcagc aggtcaaacg cgatccacac gccccacggg aagccgccgt
     9661 tcaggtcaga gacagagccc agaccgaaca ccagacgctt cacaatcagg agcatacaga
     9721 tgacgattaa cggtccaaaa atcatgaccg gtttactgat gattttgccg cccagcggtt
     9781 gtggatcatg actcatgatc gtctcctccg tcgtgatggt cgtttttggt gttgcgacga
     9841 accagcacgg ttaagcccgc cagcacagcc agtggtagca tcatgccttt atacagggtg
     9901 tgttgaatat tttcggaacg cgcaccggta gaaagatcgt ccagtttcgg caggtcgaga
     9961 ttttcataag gcacacccgt cagtaccaga acctgagtac cgccgccctc tttctcgccg
    10021 tacagatgcg gataatattt cggcaccgta tgcaggtaag tgtcgccaga tttcagcgtc
    10081 tgacgcggat agtggtattc gctgccaggc ttcagcgcca gacgtttttt cgcctccgcc
    10141 atcagctctt cacgcgtacc gaaaatcacc gcgcccgccg ggcacacttc tacgcagcca
    10201 ggcagaccgc ctttatcgag acgttccaca cctttctggt tgcacagctc gcacttatgc
    10261 agcgcaccaa acgggttgtt gtagtcgtac ttcggcacgt tgtacggaca ggcgaccatg
    10321 cagtaacggc aaccggtgca cacgtctttg tcgtaatgga caatgccggt tttcggatct
    10381 tttttcagtg cagagaccgg gcacacagag acacagttcg gatcgacgca gtgcatacac
    10441 tgtttcttaa tgtacgcgta gccgttctcc tcctggtctt tgttgacccc tgtgccgctg
    10501 gtccacacct gaatgatgtt attggtatac ggcgacagtt tgtcgttgtt cgaccaggtc
    10561 tgttcccctt gcgggttacg ttcagggaag ttgatatcct gacacttggt gacgcaagcc
    10621 tggcagccta cgcacaaggt cgagtcgtac aacatcccca gcgatcccgg aattggcggg
    10681 cggttttcag cagccgcatg actgacagac ggcagcgcgc ccgtcagcaa tgccccgcag
    10741 gaggctgctt taataaaatt acgtctgttc acggttattc tccccgtgag tcagcgttat
    10801 ctttcttttg ctgacgaccc agttcacgca ccgccatcac gctgacaccg gcaaccagcc
    10861 caaccacacc gccgagcaaa ccaatagcgc ctgcagagac gttgccgccc tctttagcgt
    10921 taacatccgg tttctgtgaa cgcggagtct gattttcgac gttggcaagc tgatggatgc
    10981 ctttatggaa gccgatacct tcttcgttac agccatagca tgggtgacca atcgctaccg
    11041 gccacacgcc gccaacatcg cagaattgca gcgttgagca gttgccgtaa gtttctggcc
    11101 ctttacagcc gaggtggtac aggcaccagc cttcacggtg gccttcatca ccgaactctt
    11161 tggcaaaacg accagcatcg aagtgcgggc gacgttcgca gtgttcgtga atcagacggc
    11221 cataggcgaa ggtcggacgg tttttgtcat ccagtttcgg cggtttgccg taagtgatga
    11281 tgtgcgcaac ggtcgcgagg aagttgtgcg ggttcggcgg gcagcccgga atattgataa
    11341 cggttttgcc tggcagaact tcttgcaggc tgactgcgcc agttgggtta actccagctg
    11401 cggcaacacc gccccacgca gagcaggaac cgatagcgat aatcgctgct gcgccttctg
    11461 ccgctttgcg gatgtgatcc acaatcggct caccagcaac catgcaataa ataccgttat
    11521 cttttaatgg gatggaacca tccacaacca acacatactg ccctttgtac ttctcaagtg
    11581 cgttatgttt gttctcttcg acctgatgac cgaaggcggc ggaaagcact tcgtgatact
    11641 ccagagagat agtctccagc acgaggtttt ctaccgttgg atgcgttgca cgaagcagag
    11701 attccgtaca accggtgcac tcctgcgcgc caatccagat aactggcgga cgctgcgggt
    11761 tagtaaccga ttcggccatc tctgcagcgg ctttgctact taaccccatg gtggcggcta
    11821 atgctgcaca aagcttcatg aaatcacgac ggttaatgcc gtgagaatgg atgagggtgt
    11881 tatctccagt catttatagt tattccgttg cgaagacctg gcatatattt tgcctcaatc
    11941 gcaaaatcaa taatgcgatc gatgcgccat ttaccacaca tttattatgg ttatcgatat
    12001 catgatactg cggcggggcg ctaaaacgaa gggaagaagc attagtgtaa ttaattaaag
    12061 caagataata cgtatgtttg atcaattttc gaggcgataa ccggccataa aaaagcgcaa
    12121 cgtagaacag gaattattct cacgaaagaa atccattacc atctctttat ccagtccata
    12181 gcggcgcgtc agaatacccg cttcccaggc actaagctgg cgatctccgg tcttttcgta
    12241 catccggtgt gagtagccta atacaaaacc gcgtttataa tcggcacaga attttgtcac
    12301 attaaccgca ctgtcggcat gcgttgcttt taatcccgcc attaagcctt taccaaaatg
    12361 gttattcatc gttcacctca ttcgctatat atcgccatat atagcgatat tttaggcaaa
    12421 gaacgacttt tgcgcacgct attttttcat gcttgtttaa gacgtaatcc agtaagagat
    12481 aaacctatca ggagagatat ttatcgaacc atttcagtgt cctttgccag gcaagatcgg
    12541 cggcagattt gtcataacgg ggcgtggaat cattatggaa tccgtgatta acccccggat
    12601 agatatacgc ctcataaacc ttattattgg ctttcaacgc cgcctcgtaa gcaggccagc
    12661 cctcgttgat tcgggtgtcc agttccgcga agtggagtag taaaggcgct tcaatcttcg
    12721 ccacatcggc agtgggtgcc tgacgaccat aaaacggcac cgcgcaggcc agttccggat
    12781 acgcgacagc cgccgcgttc gatacgccac cgccatagca aaatccggta atacccactt
    12841 tgcctgccgc ttgtggatag cgttgcataa actcaattgc ggcaaagaaa tcattcatca
    12901 gtttggttgg atcaacctgc tgttgcagct cacgaccttt atcatcattt cccggataac
    12961 ctccaacgga acttaagccg tcaggtgcca gggcgatata ccccgccttc gccactcgcc
    13021 gtgccacatc ttcgatatac ggattcagtc cacgattctc atgcaccacc accacggctg
    13081 gcgttttgcc gctcattttt gcgggtttca ccagataacc ccgtacctcg ccgtgaccat
    13141 ttggcgaagg atacatgatg tactcagcaa caatctccgg gtcggtaaac tctacctgag
    13201 tcgccagcgc ataatttggc ttgagcaaat caaacaacgc taatgccgtc atcccgccca
    13261 ccgcatactt cgccgcaagg ttgaggaact cacgtttcga aattttcccg tgagcgtaat
    13321 agtcgtagta atccaacaac tcttgtggga aatctttggc ggttaaacgc ggcatcgttg
    13381 cactcctcag ttggtgtttt tttaagcaaa gcataagcac gtgtttttgc ccagtttttc
    13441 gtcactctgt gagccagact acgggatacg cgctggcgaa tcgctaaact agaaacattg
    13501 tttcgaaatt gaacggtgga aaggagaggt catggtctgg ttagcgaatc ccgaacgtta
    13561 cgggcagatg caataccgct attgcggaaa aagcggttta cgcctgcccg cgttatcgct
    13621 cggtttatgg cacaatttcg gtcacgttaa cgcgctggaa tcacagcgtg cgatcctgcg
    13681 taaagcgttt gatttgggca ttacgcactt tgatttagcc aacaattacg ggccgcctcc
    13741 aggaagcgca gaagagaact ttggtcgcct gctgcgggag gattttgccg cttatcgcga
    13801 tgaactgatt atctctacca aggctggcta cgatatgtgg cccggccctt acggctctgg
    13861 cggttcacgt aaatacctgc tcgccagcct cgaccaaagc ctgaagcgta tagggcttga
    13921 gtatgtcgat atcttttact ctcatcgcgt cgatgaaaat acgccgatgg aagaaaccgc
    13981 ctctgcgctg gctcatgcgg tacaaagcgg taaggcgctg tatgtcggga tctcctctta
    14041 ctcgccagag cggacgcaaa aaatggtcga gttgctgcac gagtggaaaa ttccgctttt
    14101 aattcatcaa ccttcgtaca atttactgaa ccgctgggtg gataaaagcg gcctgctgga
    14161 taccctgcaa aataacggcg tgggctgtat tgcctttact cctctggctc agggattgct
    14221 gaccggaaaa tatctcaacg gcattccgca agattcacgg atgcatcgtg aagggaataa
    14281 agttcgtggt ctgacgccga aaatgcttac cgaagccaac ctcaacagcc tgcgcttatt
    14341 gaatgaaatg gcacagcagc gtggacaatc aatggcgcaa atggcgttaa gctggttgct
    14401 gaaagatgat cgcgtgacgt cggtattgat tggtgccagc cgcgcggagc aactagagga
    14461 gaacgtgcag gcgctgaata atctgacatt tagcaccgag gagctggcgc agattgatca
    14521 gcatatcgcc gatggcgagc tgaatctgtg gcaggcgtcg tccgataaat gacctgttaa
    14581 taacgggcgc gcgaaccgcg cccgaatcag atcagtgatt atgacgagtc agtcggtcaa
    14641 gatagcccat cacaaatgca gaaagcacaa acgtcagatg gataatgacg taccacatca
    14701 gtttgttatc cgggacattt ttcgcatcca taaagacgcg cagtaagtga atggaagaaa
    14761 ttgccacaat cgacgctgct actttgtttt tcagcgacgt tgcgtccatt ttccccagcc
    14821 agttcagctt ctctttgttc tcggagatat ccagctgtga gacgaaattc tcataaccgg
    14881 aaaacatcac catcaccagc aaaccgccaa ccagcgtcat atccaccagc gacagcaaca
    14941 cgaggatcaa atctgattcc gccatcgaga agatattcgg cagtacgtga ataatctcct
    15001 ggaagaactt cagcgccagg gcaactaacg ccagcgaaag gccaaagtac acgggggcaa
    15061 gcagccagcg agaagcatac attgcatttt caagaaaacg ttccatacat tcctgtcgtg
    15121 tagttaaaac gggcaacagt atatcgcaat tgagtaaaca gcacgcaaca gcaaatcaac
    15181 atatacgtta aacctttgtc gcataattct gaggtttagg gatcttcctg gttcccttat
    15241 caacgcatcg agagcgtcta cccttaaagg acgttgaacc tgaagggaga aaaacgatgt
    15301 ctcatttaaa agacccgacc acgcagtatt acactggtga atatcccaaa cagaaacaac
    15361 cgacgccagg catccaggcg aagatgacac cggtaccgga ttgcggcgag aaaacctatg
    15421 ttggtagcgg tcgcctgaaa gatcgtaaag cactggtgac agggggcgat tccggaatag
    15481 gtcgcgctgc cgccatcgct tacgcgcgtg aaggggctga cgtggcgatc agttatcttc
    15541 ccgtggaaga agaagacgct caggatgtga aaaagatcat tgaagaatgc ggacgcaaag
    15601 ccgttctgct gccaggcgat ttaagcgatg agaaattcgc ccgttcgctg gttcacgaag
    15661 cgcacaaggc gttaggcggg ctggatatta tggcgctggt cgccgggaaa caggttgcca
    15721 ttccggatat tgcagacctc accagcgaac agtttcaaaa gacctttgcc attaacgttt
    15781 tcgcgctgtt ctggctaacc caggaagcga tccccctgct accgaaaggt gcaagtatca
    15841 tcaccacttc gtcaatccag gcataccagc caagtccgca tttactggac tatgcggcta
    15901 cgaaggcggc gattctgaac tacagccgtg gcctggcaaa acaggtcgcg gagaaaggta
    15961 ttcgggtgaa tattgtcgcg ccaggcccta tctggacggc actgcaaatt tccggcggac
    16021 aaacgcagga taagatcccg cagtttggtc agaaaacgcc gatgaagcgt gcggggcaac
    16081 cggcggaact ggcccctgta tatgtttatc tggcaagtca ggagtcgagc tacgtcaccg
    16141 cagaagtgca cggcgtgtgc ggcggcgagc atttaggtta aaaaaatgcc cggttgtgaa
    16201 aagcaaccgg gcatcattgt gaattcactc ttacctgatg tggcgaaaac gccttatccg
    16261 gcctacaaac gcacgaagat tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta
    16321 gcgcatcagg caattctact ttacttcgct ttggcggttt cttcgccgac cagacctatc
    16381 ttcaggtaac ccgcctgatg cagcgtatcc attaccttca tcaacgtctc gtaatcgacg
    16441 gttttgtccg ctcggaagaa gatggtggtg tctttcttgc cttcggttaa cgcattcaac
    16501 gccgtaatca ttgtttcatc ggtgaccgga tcgttaccga taaacatcga gttgtctgcc
    16561 ttcaccgaca gataaaccgg tttttccggg cgcggctgcg gcgtgctggt agaagcaggc
    16621 aagttcacct tcacatctac cgtcgctaac ggtgccgcca ccataaagat aatcagcaga
    16681 accaacatca cgtcgataaa cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg
    16741 aggttttcgt taagatgcat tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca
    16801 cgaaccggat gcgcagcggc gctggcttcc agatccaggt cacggctttg cagcaacaat
    16861 acctgcgctg caacatcacc cagcatcgct ttaaagccgc caatctggcg tgcaaatacg
    16921 ttatagataa cgaccgcagg aatcgctgcc acgaggccga ttgccgttgc taacagagct
    16981 tctgcgatac ccggcgcaac gactgccagg ttagtggtct gcgtttgcgc gataccaata
    17041 aagctgttca tgatgcccca gaccgtacca aacagaccaa cgaacggaga aatcgcgccg
    17101 atggttgcca gatagccgtt accgcggccc atttgacgac ccactgcggc gacccgacgc
    17161 tccagacgga agctggtacg ttctttaata ccttcgttat cgtcgctgcc ttctgacagt
    17221 tccagctcat tctgcgcttc attgagcaaa tgcaggctta agcttttgct accaaaatca
    17281 gcggcgatat cgttggcctg gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc
    17341 ttaaggcgac gcttctgatt gaagaactct acgctcttac tgaagaagat tgcccaagtg
    17401 actacggagg ccaaaataag cccaatcatc acgcacttaa cgacaatatc ggcgtgctga
    17461 tacatacccc agacggaaag gtccgtctgc atcaaattat tacccactgt gtatctccag
    17521 gacgcaagtc acaaaatctg cgcataataa tatcaaaatg acgtcgaatt gatagtcgtt
    17581 ctcattacta tttgcatact gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa
    17641 aaaagtcacc agcacgccat ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc
    17701 gcccgcgaat attcatgcta gtttagacat ccagacgtat aaaaacagga atcccgagat
    17761 ggcggacaaa aagcttgata ctcaactggt gaatgcagga cgcagcaaaa aatacactct
    17821 cggcgcggta aatagcgtga ttcagcgcgc ttcttcgctg gtctttgact gtgtggaagc
    17881 caaaaagcac gcgacgcgca atcgcgccaa tggcgagttg ttctatggac ggcgcggaac
    17941 gttaacccat ttctccttac aacaagcgat gtgtgaactg gaaggtggcg caggctgcgt
    18001 gctatttccc tgcggggcgg cagcggttgc taattccatt cttgcttttg tcgaacaggg
    18061 cgatcatgtg ttgatgacca acaccgccta tgaaccgagt caggatttct gtagcaaaat
    18121 cctcagcaaa ctgggcgtaa cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt
    18181 taagcatctg cagccaaaca ctaaaatcgt gtttctggaa tcgccaggct ccatcaccat
    18241 ggaagtccac gacgttccgg cgattgttgc cgccgtacgc agtgtggtgc cggatgccat
    18301 cattatgatc gacaacacct gggcagccgg tgtgctgttt aaggcgctgg attttggcat
    18361 cgatgtttct attcaagccg ccaccaaata tctggttggg cattcagatg cgatgattgg
    18421 cactgccgtg tgcaatgccc gttgctggga gcagctacgg gaaaacgcct atctgatggg
    18481 ccagatggtc gatgccgata ccgcctatat aaccagccgt ggcctgcgca cattaggtgt
    18541 gcgtttgcgt caacatcatg aaagcagtct gaaagtggct gaatggctgg cagaacatcc
    18601 gcaagtagcg cgagttaacc accctgctct gcctggcagt aaaggccacg aattctggaa
    18661 acgagacttt acaggcagca gcgggctatt ttcctttgtg cttaagaaaa aactcagtaa
    18721 tgaagagctg gcgaactatc tggataactt cagtttattc agcatggcct actcgtgggg
    18781 cgggtatgaa tcgttgatcc tggcgaatca accagaacat atcgccgcca ttcgcccaca
    18841 aggcgagatc gattttagcg ggaccttgat tcgcctgcat attggtctgg aagatgtcga
    18901 cgatctgatt gccgatctgg acgccggttt tgcgcgaatt gtataacatt gccacttttg
    18961 gacaattttg cagacattta attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc
    19021 gtcccggacg aatcaggagt acaataggca gataaaggct taaacgctgt tccacaggaa
    19081 agtccatggc tgttattcaa gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc
    19141 tggcggatcc tcatattgtt agcgttgttt actttgtcat gtttgccacg ctgtttttag
    19201 aaaacggcct gctgcccgcc tcatttttgc caggcgacag cttgttgata ctggcaggcg
    19261 cattgattgc ccaaggggtt atggattttc tgcctacgat tgcgattctg accgccgcag
    19321 caagtctggg ctgctggcta agttatattc aggggcgctg gttagggaat accaaaacgg
    19381 tgaaaggctg gctggcacag cttcctgcta aatatcacca gcgcgccacc tgcatgtttg
    19441 accgccacgg tctgctggcg ctgctggctg gacgttttct tgcatttgtc cgtacgctgc
    19501 tgccaaccat ggcgggaatt tccggtctgc caaaccgccg cttccagttt ttcaactggt
    19561 taagtggatt gctgtgggtc agcgtggtaa ccagttttgg ctatgcctta agtatgattc
    19621 cgttcgttaa acgccatgaa gatcaggtaa tgacgttcct gatgatcctg ccaattgcct
    19681 tgttaaccgc tggcttgtta ggcacgctgt ttgtagtgat taaaaaaaaa tactgtaacg
    19741 cctgacgatt ttccccgttc ccggctgctg taccgggaac gtatttaatt cccctgcatc
    19801 gcccgcattc ttgccgcatc ttcccccggc gtcacaccga agtaacgttt aaactcacgg
    19861 ctaaattgcg atgcgctttc atagtcgacg cgcatcgctg ctgcgctggc cttcatgccg
    19921 tcatggatga tcatcatccg cgccttatgc agacggtaat tcttcaaata ctgcaacggc
    19981 gaggtgctgg tgacagactt aaaattatga tggaacgccg atacgctcat gttggcttct
    20041 gccgccagtt gctcgacgct caggttttcg gtgtatttat tctcaatccg tttcagcacg
    20101 cggctaatca gactgaagtg agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa
    20161 ggtccggtca gcacgtagta cagaatttcg cggatgatct gtttgccgag aatacgcgca
    20221 tccagtggtc gctccatcac gtcgagtaac cgctccgccg cgcataaaat ctcttctgat
    20281 aacgtggcgg agttaatccc gctggctgcc atcgacggct ggaaatgctc atcttcgcca
    20341 atgtccatca acagttcctg taactgcaaa atatcgacat tgagacgcaa tcctgccagc
    20401 ggcacctctg acgtggcata ggtttcgcac tcaaacggca acggcaccgt cagcagcagg
    20461 tattcattgg catcataacg aaacacgcgt tcattgatat aaccgatttt atgcccggaa
    20521 aagagaatta tgatgccagg ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca
    20581 tacaacaaac gcacatcggg caacagttct gacaaactat tttctttatt tttcagttta
    20641 ttaactttat ccgccagcaa gcggcaaatc tcttcacgtt tcatatcgcg taatttctta
    20701 ggaataatgc ggcaatttga ttgtgcgcaa ttttgtagca tttctccagc actctggaga
    20761 aataggcaag acattggcag aaatgagcat tgagagccag ggcgctggcg atcacaatga
    20821 aaaacatcag gcagatcgtt ctctgccctc atattggccc agcaaaggga gcaagtaatg
    20881 aacaacttta atctgcacac cccaacccgc attctgtttg gtaaaggcgc aatcgctggt
    20941 ttacgcgaac aaattcctca cgatgctcgc gtattgatta cctacggcgg cggcagcgtg
    21001 aaaaaaatcg gcgttctcga tcaagttctg gatgccctga aaggcatgga cgtgctggaa
    21061 tttggcggta ttgagccaaa cccggcttat gaaacgctga tgaacgccgt gaaactggtt
    21121 cgcgaacaga aagtgacttt cctgctggcg gttggcggcg gttctgtact ggacggcacc
    21181 aaatttatcg ccgcagcggc taactatccg gaaaatatcg atccgtggca cattctgcaa
    21241 acgggcggta aagagattaa aagcgccatc ccgatgggct gtgtgctgac gctgccagca
    21301 accggttcag aatccaacgc aggcgcggtg atctcccgta aaaccacagg cgacaagcag
    21361 gcgttccatt ctgcccatgt tcagccggta tttgccgtgc tcgatccggt ttatacctac
    21421 accctgccgc cgcgtcaggt ggctaacgac gtagtggacg cctttgtaca caccgtggaa
    21481 cagtatgtta ccaaaccggt tgatgccaaa attcaggacc gtttcgcaga aggcattttg
    21541 ctgacgctaa tcgaagatgg tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc
    21601 gccaacgtca tgtgggcggc gactcaggcg ctgaacggtt tgattggcgc tggcgtaccg
    21661 caggactggg caacgcatat gctgggccac gaactgactg cgatgcacgg tctggatcac
    21721 gcgcaaacac tggctatcgt cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc
    21781 gctaagctgc tgcaatatgc tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag
    21841 cgtattgacg ccgcgattgc cgcaacccgc aatttctttg agcaattagg cgtgccgacc
    21901 cacctctccg actacggtct ggacggcagc tccatcccgg ctttgctgaa aaaactggaa
    21961 gagcacggca tgacccaact gggcgaaaat catgacatta cgttggatgt cagccgccgt
    22021 atatacgaag ccgcccgcta agctttttac gcctcaaact ttcgttttcg ggcatttcgt
    22081 ccagacttaa gttcacaaca cctcaccgga gcctgctccg gtgagttcat ataaaggagg
    22141 aacgtatggc taatccaacc gttattaagc tacaggatgg caatgtcatg ccccagctgg
    22201 gactgggcgt ctggcaagca agtaatgagg aagtaatcac cgccattcaa aaagcgttag
    22261 aagtgggtta tcgctcgatt gataccgccg cggcctacaa gaacgaagaa ggtgtcggca
    22321 aagccctgaa aaatgcctca gtcaacagag aagaactgtt catcaccact aagctgtgga
    22381 acgacgacca caagcgcccc cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg
    22441 attatatcga cctctactta atgcactggc ccgttcccgc tatcgaccat tatgtcgaag
    22501 catggaaagg catgatcgaa ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca
    22561 acttccagat ccatcacctg caacgcctga ttgatgaaac tggcgtgacg cctgtgataa
    22621 accagatcga acttcatccg ctgatgcaac aacgccagct acacgcctgg aacgcgacac
    22681 acaaaatcca gaccgaatcc tggagcccat tagcgcaagg agggaaaggc gttttcgatc
    22741 agaaagtcat tcgcgatctg gcagataaat acggcaaagc cccggcgcag attgttatcc
    22801 gctggcatct ggatagcggc ctggtggtga tcccgaaatc ggtcacacct tcacgtattg
    22861 ccgaaaactt tgatgtctgg gatttccgtc tcgacaaaga cgaactcggc gaaattgcaa
    22921 aactcgatca gggcaagcgt ctcggtcccg atcctgacca gttcggcggc taacatgcaa
    22981 attctcccgg tgacggtaat gttccgctac cggacttttc agaaatcatt tattcccctc
    23041 gcgtcccgcc cgttgttact cttccttgtt caggaatgcc aaatataagg acatcatcat
    23101 gcagagccgg aagctcttaa aagaacaact catctatatc cgggataaac gcaacggaga
    23161 ggtgaaaaac agatgaaaat aatacttctg tttttagcag ccctggcaag ttttaccgta
    23221 cacgcgcagc ctccttcaca gaccgtagaa caaacagtcc ggcagattta tcagaactat
    23281 aaatcagatg ccagtacacc ttatttcggt gaaaccggag agcgggcgat aacttcagcg
    23341 cgtattcaac aggcgcttac cctgaacgac aatcttacgc tgccgggcaa tattggctgg
    23401 ctggattatg atccggtttg tgattgtcag gattttggcg atctggtgct agaaagcgtt
    23461 gcgataaccc aaactgacgc cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa
    23521 gatgataaag aaaagaccac gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc
    23581 attgacgata ttgtcagcaa tcatggcagc gtcttacaag cagttaatag cgagaatgaa
    23641 aaaacgctgg ccgctttagc ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa
    23701 ctctttgaac atattgctga ttatagctgg ccgtggacgt gggtggtttc cgactcttac
    23761 cgccaggcgg ttaatgcctt ctataaaacc accttcaaga cggccaataa tcccgatgaa
    23821 gatatgcaaa tagaacggca atttatttac gacaatccga tctgttttgg cgaagagtcg
    23881 ctattttcac gcgttgatga aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt
    23941 catgttcgtt ttacgctgac caatggcaac aacgaagagc aagaactggt tttacagcgg
    24001 cgcgaaggca agtgggaaat cgctgatttt atccgcccga acagcggcag cctacttaag
    24061 cagattgagg caaaaaccgc cgccagatta aagcaatgag ctgaattaaa taacaattag
    24121 ccggaacaat aaataaaagg gaacactata tgaaaacgat tttcaccgtg ggagctgttg
    24181 ttctggcaac ctgcttgctc agtggctgcg tcaatgagca aaaggtcaat caactggcga
    24241 gcaatgtaca aacattaaat gccaaaatcg cccggcttga gcaggatata aaagcactac
    24301 gcccacaaat ctatgctgcc aaatccgaag ctaacagagc caatacgcgt cttgatgctc
    24361 aggactattt tgattgcctg cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc
    24421 ggcagcatgt cagttgccgg ggattttttt taacgtccaa ccgccgcttt agggcgtttc
    24481 ttcgcaccag cattcaccgg acgagattgc gtagacgacg ctttttttgc cgtagcaggc
    24541 gtctgacgct gggtcgccat cggcgtatgt ttagtcaacg ccggacgggt attgcggttc
    24601 tggcgacgag cttcacgcat ctcttcaatg gttggcgcag gcactaagca atcgcgacgg
    24661 ctgccaatca gatgcttttt gcccatcgct tccagcgccg ggcggattaa cggccagttt
    24721 gccggatcgt ggtaacgcaa caacgcttta tgcaaacgac gctgtttgtc gcccttcggt
    24781 acgaagacgt cttcgctctt ataaccaatc ttcgccagcg gttttttccc ggtgtaatac
    24841 atggtggttg agttcgccag cggcgatggg tagaagttct gtacctggtc gagacggaaa
    24901 cgatgctttt tcagccacag cgccaaattc accatatctt catcacgcgt accggggtgc
    24961 gcagagatga aatacgggat cagatactgc tctttacctg cctgtttcga gtaggtatcg
    25021 aacagctctt taaagcggtc atagctgccc atgcccggct tcatcatctt cgacaacggc
    25081 ccttcttcgg tatgttccgg agcaatcttc agataaccgc cgacgtgatg ggtcgccaac
    25141 tctttgatat agcgcggatc ttccacggcg atgtcataac gtacgcctga ggcgatgagg
    25201 atttttttaa tgcctttcag atcacgcgca cggcgataga ggttgatcgt cggttcgtgg
    25261 ttagtgtcca tgtgcggaca aatatccgga taaacgcacg acaaacggcg acaagtttgt
    25321 tcagcgcgtg gcgatttgca gtgcaacata tacatgttgg cagttggccc accgagatcg
    25381 gaaatcacgc ccgtaaaacc tggaacggtg tcgcggatcg cttcgatctc attaatgatc
    25441 gaatcttccg aacggctctg aataatgcgc ccttcgtgct cggtaataga acagaaagaa
    25501 cagccgccaa agcagccgcg cataatgttg accgaaaaac ggatcatttc gtaagccgga
    25561 atacgggcat tgccataggc cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg
    25621 tccatctctt cggtagaaag cgggatagca ggcgggttga tccacacata gcggtcaccg
    25681 tgtttttgca tcaatgcgcg gacgcagcct gggttggttt cgtggtgcag aatacgcgaa
    25741 gcatgggcgt acagcacttt atcgcccttc actttctcga aagaaggcag caacacgtag
    25801 gttttttccc acggtttcgg gcgcggtggc tgcacggtta cggctttggc ttcctgcttt
    25861 ttcggtgcca ccggtttgtt atccgcgcac ggcaaatctt caccatacgg atgcgggatt
    25921 gggtcgattt ttccaggggt atcaagacgg ggggaatcca cgccgctcca gccaggcagc
    25981 gcctctttca cgataatcgc ggtattacgc acatcgcgga tttcactaat cggctcgccc
    26041 atcgccagac ggtgcgccac ctccaccagc ggacgctcac cgttaccaaa catcagcatg
    26101 tcggctttcg aatccaccag cacggaacgg cgcacggtat cggaccagta atcataatgt
    26161 gcggtacggc gcagactagc ctcaataccg ccgaggatca ccggtacatc tttccacgcc
    26221 tctttacaac gctgggtata aaccagtgtg gcgcgatccg ggcgcttacc cgcgacgtta
    26281 tccggcgtgt aggcatcgtc atgacgtaaa cggcgatcgg cggtataacg gttgatcatc
    26341 gaatccatgt tgccagcagt aacaccgaaa aacagattcg gtttacccag acgcataaag
    26401 tcgtctttgc tgctccagtc cggctgggcg atgatcccga cgcgaaagcc ctgtgcttcc
    26461 agcatacgac cgcaaatcgc catcccgaag cttgggtgat cgacatacgc gtcgccagta
    26521 accaaaatga tgtcgcagct atcccagcca agttgatcca tctcttcacg agacatcggc
    26581 aaaaacggtg ccggtccaaa acaggcggcc cagtactgcg gccaggagaa caggtcgcga
    26641 tccggttgga tcagggagat agagctcatt ttgcttccag aaatgataaa aaaataatca
    26701 aaggccgggg attataagcc ggaacgaaag agaaatcgaa aagtattcca tactcgccct
    26761 cctcgggcga gtatgaagat tacggtaccg gattgactaa cagttgccca atcgagccac
    26821 ggtccgccat ttccagcgtc tgactgttga agtagaacgg gaagtgcgcc caggaaggct
    26881 gaccgaaata aacaagcagc tccacttgtc cgtcaaccca aacggtatct ttccagcctc
    26941 tgtcttccgg gaacggcatc gcaccattca cgttacggat ctggaacatc acgccttcaa
    27001 tatggaacgc ttgcggctcg tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg
    27061 cggtgacatc aatacggttg acgtcccaca gctgtccatt aatacccggg tcatcaccca
    27121 gactgatatc gcgactgcga atcggcgaac cggccatgat ttcagttggc agcaagcgca
    27181 tcggaagact gtctgtgacc agcggcagaa ggccggttgg gcgcagcgtt agcaccaggg
    27241 tagaaaccag aatactggat ggctcaaaga agccgcgaat acgatcaaca atgctcgccg
    27301 cttcgccaca ggtgatcgac acttcatcgc cgttgctcat atccaccaga atctcgcggc
    27361 gctcgcccgg tgccagcgaa agttgcttca ccgatacagg agcaggcagg aatccctgat
    27421 cgccagaaat cacatgtaac gggcgaccat cgctcatctg tagttgatag cgacgagagt
    27481 tcgacgcgtt cagcagtcgc aagcgcaccc agccacgcga gacttcaacg tacgggcttt
    27541 gtacaccgtt aaccagcagc gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt
    27601 ctggcgtacc aaagttatcc agccgtttat cctggataat gaccggaaaa tcatccacat
    27661 cataatggtt ggggataggc agcgacttgc tgacttcatc ttccaccagc cacattccgg
    27721 caaggccgtt atagacctgc tgagccgtgc ggttgggagt attggcgtga taccacagag
    27781 tagctgcgtt ctggcgaata ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc
    27841 gtgccggacc gcccatcagc gggccaggta cctgtagccc ggccaccgtc attgagacat
    27901 tttctgtcag gcggttgctg taaataagct taacatcgtc gcccttccag acgcggatag
    27961 tcggccccag gtaacgacca ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg
    28021 accagtgcgc acgttgtaca gtcataaaca gcggttgccc acggcgagat tcaagtagcg
    28081 gcggaacggg tagcggttgc tgttgcccgg ctgcgctggc cttcagggga acagcgcctg
    28141 cacaaagtgc aatccccgat gcctgaatga actgacgccg actgagtgac atatttgctc
    28201 catgtaaaac tggctaaagt aataccgtgc aggaaagtgt tccccgcaaa aatacagatt
    28261 gccgacttaa acttttccgg cggcttcgcg ttctgcgact tctttatcga gctcggcgat
    28321 tttttgttcc attatcgaac gacaatgggc agccagctca cgaacctgat ctttgccata
    28381 ctgactgacg tcaattggcg gcagcatttc gacaatcacc agaccgttgt gcagtcgatt
    28441 aagattaatc ttattcgaag ttgtagagac gcacacggga ataatcggga cgcccgccgc
    28501 aattgccgcg tgaaatgctc cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt
    28561 tccttccggg aacatccaga tggaaatacg gcgttttttg aaatgattca cgacttccgc
    28621 aatggtgccg tgagctttag tgcgattgtt tctgtcgatc aataagttgc cggttaacca
    28681 gtacaactgc ccgaagaagg ggatccacag caagctcttt ttacctaccg tcaccgtcgg
    28741 cggttgcaca atgttcgatg ctgtcaccat gtcatagttg ttctggtggt tagcgatata
    28801 gatagcattg ccgtagcttt cagcatctgc aggtttgcgg cactcaactt tcaggccgaa
    28861 cagcggcgca agacggccaa acatatgccc aaaggtggcc acatgtttcg ggttacgcgg
    28921 gctgaaaagg cagtaaatgg agccgaatac acagactaag atgctgtaaa tcacggtaat
    28981 aataagacga aagatatata gcataacacc ctctgaaggt ctgagagact ggcattgtac
    29041 gtcacctgaa atcaggttag ggcattgtta aaagccgctc tctggaaaag cgtattgtta
    29101 atcgcaacgc atgaataaac aacggtttca agggaatttt tatcgtttcc gggggttgaa
    29161 tacgctgccg gatgacgact taacgtttca tccggcgttc cttgcaagcg ggaggaaaca
    29221 ggccctcccc ggcatattac tcttcgctat caccgttgct ggcacggcga ggagagtcga
    29281 tctcaacacg atcgatacgc tgcaaaccgc gcatcaacgt accgcggcgt ccacgttcac
    29341 cagtgacttt ctgtaactct tccgggcgca gtttaatttt gcgtttccca acatgaatgg
    29401 tcagcgtgct ttgcggcggc agaacgtaca attgcgccag accatcttct ccacgcgcgg
    29461 cttctgccga tggaatgttg ataatcttgt tgcctttgcc cttcgacagc tgcggcagat
    29521 cgcttaccgg gaacatcaac atacggcctg cctgagtgat tgccagcagc atatcggaag
    29581 catcttcaat caccaccggc ggcataacat gggcattttc cggtaaggtg atcaaagcct
    29641 tacctgcacg gttacgcgcc accagatcgt taaaggtgca gacgaaaccg taacccgcat
    29701 cggaagccat cagcagtttc tgatcgtcgc tttccatcag catatggtca acggtcgccc
    29761 caggcggcaa cgttaatttg ccggtgagtg gttcgccctg accacgcgcc gacggcagcg
    29821 taatcgggtc gatggcatag ctacgaccgg tggaatcaac aaacactacc gattggttgc
    29881 tcttaccttt caccgccact ttgaagctat cacccgcttt ataattcagg cccggcgcgt
    29941 cgatatcatg gcctttagcg ctgcgtaccc agcccatctg cgacagcaca atggtgacag
    30001 gttcagacgg cagcatgtcg tgctcgctca tcgctttcgc ttcttcgcgt tcctgcaacg
    30061 gcgaacgacg atcgtcaccg taggcttgcg cgtctgcctg cagttctttc ttcagcaggt
    30121 tattcatttt acgctcggaa gccaaaatgc cctgcaactg gtcgcgctct ttttccagct
    30181 cactctgctc accgcgaatc ttcatctctt ccagtttggc aagatgacgc agtttcagtt
    30241 cgaggatcgc ttccgcctgg gtttccgtaa ggccaaaccg cgacatcagc gccggtttcg
    30301 gttcatcttc gttacgaatg atctcaatca cttcgtcgat gttgagaaac gccaccagca
    30361 aaccttccag gatatgcagg cgcttgagga ctttctccag acgatagttc agtcggcggc
    30421 gcacggtatc gcgacggaac accagccatt cggagaggat ttccagcagg tttttcaccg
    30481 ccggacgacc atccagaccg atcatattga ggttaatgcg atagctcttt tccagatcgg
    30541 tggtagcgaa gaggtggttc atcacctgat ccatatccac gcggttggaa cgcggcacaa
    30601 tcaccagacg ggtcgggttc tcgtggtcag attcatcgcg cagatcgtca accatcggca
    30661 gctttttgtt gcgcatttgc gcagcaattt gctccagtac acgcgcacct gaaacctgat
    30721 gcggtaatgc gctgataacc accgcgccat cttctttctt ccacaccgcg cgcatacgta
    30781 ctgaaccacg tccgttctcg tagattttac ggatctcggc gcgcgaagtg ataatttccg
    30841 cttcagtcgg ataatccggc ccctgcacga tatccagcag ctgatcgagc gtggttttcg
    30901 gctggtcgat taatgcgatt gccgcctgag ctacttcacg caggttatgc ggtggaatat
    30961 cggtcgccat gccgacggca ataccggtgg tgccgttaag caaaatgttt ggcagacgag
    31021 caggcagcat tttcggctcc tgcaaagtgc cgtcgaagtt tggcacccag tcagccgtcc
    31081 cctgccccag ctcgctcaat agcagctcgg aatatttcga caaccgggat tcggtgtaac
    31141 gcattgccgc gaacgatttc ggatcgtccg gcgcgcccca gttcccctga ccatcaacca
    31201 gcggataacg gtaagagaac ggctgcgcca tcaggaccat cgcttcataa caggcgctat
    31261 cgccgtgcgg atggtattta cccagtacgt caccgacggt acgggccgat tttttaaatt
    31321 tggcgctggc attcaggccc agttcagaca tcgcatacac aatgcggcgc tgaacaggtt
    31381 tcagaccatc accaataaac ggcaacgcac ggtccatgat cacgtacatg gagtagttta
    31441 agtaggcgtt ttccgtaaat tcatgtagcg caaggcgctc tgccatatcg ctcattaatt
    31501 ctgattcctc aactgattcg ccagccttca aaggggcaat attgccggag atactacatt
    31561 atctgccgcg tcgagtcaca ggagatcagc gtgaatagcg ccgccggacg tgctccgacg
    31621 gcgataaagg catcaatgtg ccttgatata caacgctttg ctgtagactt gatcggcata
    31681 gagggcagca caaggttgcg cccatgccac accactcggc atgggggtcc gctccgggcg
    31741 attcgagaag ttgaggttat gcgagtccag ggaagcctaa agcatcaaag tagtcgacta
    31801 taagtatatg gttcagccca gtttagctgt gtctccacca gtgccccgcg cctcctgcca
    31861 taagtgcggc ttgctcatgg gtacccccga gacggcttac ggcgcaccgg ataataaatt
    31921 tcgcgtatac atggtattcc ttaccggaga aattataagc ccaacaacaa tacgcctgtg
    31981 gcattgttcg ctcaaactta tcgcgagtga tttggctcac actccggtaa agattcccca
    32041 tcacgctaac ggcacataaa catccgtttc cagtcgctgc tccgtcaccc cttcggcaag
    32101 attggtgtag tgaaacagaa tcggtgcttt acgcattttc tcgccgcttg caggcagcca
    32161 gtggcgaata atgccccata tcgtgtgtga aatatcgtcc agctcgccaa cgtggcgggc
    32221 cacggcataa cgtccaccgg taagttcacc attgctcaca ccataacgat tatcgggaat
    32281 cggttcgcta acgctgccgc agatatcaaa gcgaaacgct tccggtgcgg tggtggcagg
    32341 gtcgtcccag gcgacgccaa aagtctggct ttggttaaca ggagaaagcc ccgtctcttt
    32401 acgccacatg ataaatttcg ccacactgta gttgagcaac tcagggctac agcggtgcgg
    32461 caacatcgcc acagggattg atgggaaatc gataatgttt acatccagtg tcaggtttgt
    32521 cattttgcct ccctgcgaaa ttattcccgg gaacgcgtcc cgggagcggt aacaattacg
    32581 gatttacttt gcggatctgt ttgacgtcaa tttcaacaga attccagtct ttatcgactt
    32641 caccctgaat ctcaaccgta tctttcggcg tcaccgtcac accgttccag cgtttgtggt
    32701 cgatatcaac attgatagta ccgctggcat ctttaaacac gtagagatcg tcagagatgc
    32761 gttcaacgat attgccgcgc agggttaccc aggtgtcgtc acgcagggat tttgcgcttt
    32821 ctacagtcgt tacgctgccg ttcggcccct ggaatcctcc ggcctgactt tgcgttgccg
    32881 atgggccaga aaaaccgccc tgctctgctg ccatcaccgg tgcactgcac agggccatta
    32941 ctgcgattac tgctgcgaat tttttcatgt ttattactcc ctttaatgtc tgtttccgag
    33001 catttaacaa gatagtcctt aacaacttct taagggaaaa aaataaaatt taatgctgta
    33061 cagagcgcgt tacaacacgg tttactggca gcaaatacgg ttatcgcagg gatgaaaaaa
    33121 tgcgaatttt actgatagaa gatgacatgc tgattggcga cggcatcaaa actggcctta
    33181 gtaaaatggg ttttagtgtc gactggttta cacaaggtcg tcagggaaaa gaggcgcttt
    33241 atagcgcacc ttatgatgcg gtgatcctgg atttaacctt accaggcatg gatggtcgcg
    33301 atattttgcg cgaatggcga gagaaaggtc agcgtgagcc ggtactgatc ctgaccgcgc
    33361 gtgatgcgtt agcggaacgt gtagaagggc tgcgtctggg agctgacgat tatctgtgta
    33421 aaccttttgc gttgatagaa gtcgccgcca ggctggaggc tctgatgcgc cgaaccaacg
    33481 gccaggccag caacgagctg cgccacggta acgttatgct cgaccccggc aaacgtatcg
    33541 ccacgctggc tggcgaaccc ttaacactga aaccaaaaga atttgccctg ctggaattac
    33601 tgatgcgtaa cgctggtcgg gtactgccgc gcaaactgat tgaagagaaa ctgtatacct
    33661 gggacgaaga ggtcaccagt aatgccgttg aagtgcatgt gcatcatctg cgacgcaaac
    33721 tcggtagtga ttttattcgt accgtgcatg gtattggcta cacattaggt gagaaatgaa
    33781 atttacccaa cgtcttagtc tgcgcgtcag gctgacgcta atctttttaa ttctggcctc
    33841 ggtgacctgg ctgctttcca gctttgtcgc ctggaaacaa acaacggata acgtcgatga
    33901 attgttcgac acccaactga tgctgtttgc caagcggtta agtacgctcg atctcaacga
    33961 aatcaacgcg gcggatcgca tggcacagac gccaaataaa ttaaaacacg gtcatgttga
    34021 tgacgatgcg ctgacctttg ccatctttac ccacgacggc agaatggtcc ttaatgatgg
    34081 cgataacgga gaagatattc cctatagcta tcaacgggaa ggttttgctg acgggcaact
    34141 ggtcggtgaa gacgatcctt ggcgttttgt ctggatgacc tcacctgatg gcaaatatcg
    34201 catcgttgtt ggccaggaat gggaataccg tgaagacatg gcgctggcga ttgttgccgg
    34261 gcaattgatc ccgtggctgg tcgcactgcc gattatgtta atcatcatga tggtactact
    34321 gggtcgtgaa ctcgcgccgc tgaacaaact ggcgctggca ctacgtatgc gtgaccctga
    34381 ctcggaaaaa ccactaaacg cgactggcgt acccagcgaa gtgcgaccac tggttgagtc
    34441 gctaaatcaa ctgttcgccc gcacacatgc gatgatggtt cgagaacgac gctttacctc
    34501 cgatgcagct cacgaactac gtagcccgtt aacagcgctg aaagtgcaaa ccgaagttgc
    34561 gcagctctct gacgatgatc cgcaggcgcg gaaaaaagca ctgctccaat tacattccgg
    34621 gatcgatcgc gctactcgtc tggttgatca actgctcacg ctatcgcggc tggactcact
    34681 ggataacctt caggacgtcg cggagatccc gcttgaagat ctcctgcaat cgtcggtgat
    34741 ggatatttac cacacggcgc agcaggcgaa aattgacgtg cgactgacac tcaatgccca
    34801 cagcatcaaa cgcactggac aaccgctatt gctaagtttg ttggtgcgaa accttctgga
    34861 taacgcagtg cgctacagtc cacagggcag cgtggtggac gtcacgctga atgccgataa
    34921 tttcatcgtg agggataacg gccccggtgt gacaccagag gcactggcgc gaattggcga
    34981 acgcttctat cgcccccccg gacaaaccgc taccggcagt ggacttgggc tatcgattgt
    35041 ccagcgaatc gccaaattgc atggcatgaa tgttgaattt gggaatgcgg aacaaggtgg
    35101 atttgaggcg aaggtgagct ggtaagggta agacttttgc taaattcgca aaagactttg
    35161 cacattttgc taattttacc gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    35221 cttgaggtta aaaaatgagc aacaccctga ttatcaacgg cgcgaaaaaa ttcgcccact
    35281 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    35341 ggcatgatgt tcgcatcgtt cgcgccgaca gcgactacga tgtcaaagcg gaagtacaaa
    35401 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    35461 ggacagtgaa aaaatacatt gatgatgtat tcaccgaagg tcacgggacg ctgtatgcca
    35521 gcgatggccg tacccgcaaa gatccgtcaa aaaaatacgg ttccggcggc ctggtacagg
    35581 gcaaaaaata tatgctttct ctgacctgga acgcaccaat ggaagccttc accgaaaaag
    35641 atcaattctt ccacggcgtt ggcgtcgacg gtgtgtatct gccgttccat aaagcaaacc
    35701 aattccttgg catggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    35761 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    35821 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    35881 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    35941 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    36001 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    36061 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    36121 tgctggagat gaatattcgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    36181 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    36241 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    36301 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    36361 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    36421 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    36481 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    36541 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    36601 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    36661 gaagctggct cagatcgtcg ctgttaggat cgataccgat cgctaccgaa atatcatgca
    36721 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    36781 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    36841 agtcaccttc cacgaggaac agttcggtac ggttaaggtc ctgcgcggta caatcagcca
    36901 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    36961 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    37021 tctggttcag ccacaggata aaggcatctt tcaccacgcc agaaacgaat gccgcgcatt
    37081 gacgcgaaga gagacgctct ttcgtctgcc cggcaaactg cggatcctgc attttcaccg
    37141 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    37201 gaatgttgcg atattcacag aactcacgca tcgcgtccaa caggccctga cgcaggccat
    37261 taacatgggt accgccctgc atcgttggga tcaggttgac gtagctttcg gtcagcagtt
    37321 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    37381 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    37441 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    37501 taatctcaac gccggggcac agtaccgctt tggctttcag cacatgcgtc aggcgtgaaa
    37561 cagaaaatcg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    37621 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    37681 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttcaacgcgc ttcgacaggg
    37741 cgttaaccac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    37801 agaatttacc gcctgcatgc agacggcaaa gaatcaattc aaccgccggg acaccctctt
    37861 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat aacttctaac gactggtcag
    37921 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    37981 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    38041 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    38101 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    38161 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    38221 tggcggcagg aagcgtagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    38281 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    38341 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtatgggtt ctcgttctga
    38401 ccgagatagt ccgtcagcag ttcaaacggg cgcaccgccg ggtttaccac cactgcgggc
    38461 agcataaaac attgtgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    38521 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    38581 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    38641 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    38701 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    38761 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    38821 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    38881 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    38941 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    39001 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    39061 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    39121 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    39181 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    39241 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    39301 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    39361 caatcaggtc gaattcgtgc tgatgtggac gaatcgcctc cagcaccgcc tggtagcttt
    39421 cccaggtgtt tacccctaac agagcttcgt gcttttgtgc aaacaggtga gtgtcggtaa
    39481 tttgtaaaat cctgactctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    39541 ggtgtcctta ggtttcacga tgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    39601 agtatcgcaa ccagtccgct aaaaactgat taatttgatg cttttcgtcg cgttgatgca
    39661 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    39721 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    39781 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    39841 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    39901 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    39961 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    40021 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    40081 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    40141 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    40201 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    40261 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcta
    40321 tcatcccggc aaccatctcc agtagccagg gggtttcact ggtgtcgtat gcggcaatcc
    40381 gaatctgctc aatcagcaca acttcatcgc gcactgggtc aaagggtagc aagactgcgg
    40441 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    40501 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    40561 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    40621 atgtcctggc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    40681 gcagtcatgt gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    40741 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    40801 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    40861 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    40921 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    40981 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    41041 cggctaccgc gacgcgaacg gcatcaactc taacgcgacc agtgcgtccc tgcagttaac
    41101 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    41161 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    41221 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    41281 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    41341 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    41401 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    41461 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    41521 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    41581 ggcgcgcgag caaattcgcc aggcgcagga tggtcactta ccgacgctgg atttaacggc
    41641 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    41701 ccagtatgac gacagcaata tgggccagaa caaagtgggc ctgagcttct cgctgccgat
    41761 ttatcagggc ggaatggtta actcgcaggt gaaacaggcc cagtacaact ttgttggtgc
    41821 cagcgagcaa ctggaaagcg cgcatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    41881 caacattaat gcatctatca gtagcattaa cgcctacaaa caagccgtag tttccgctca
    41941 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    42001 gttggatgca accaccacgc tgtacaacgc taagcaagag ctggcaaatg cgcgttataa
    42061 ctacctgatt aaccagttga atattaagtc agctctgggt acgttgaacg agcaggatct
    42121 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcacc
    42181 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcaccagt
    42241 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    42301 acgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    42361 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    42421 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    42481 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    42541 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    42601 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    42661 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    42721 atttggtgaa ggtcagtgcc agcaggcacc agcccaggct ggcatggcac cagaaaacca
    42781 ggcgcaggcc cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    42841 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    42901 cccagccagc ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    42961 ccagccaggc cgcaccatga ccgtaccgaa gacagccatg gcaccaaaac cggcgaccac
    43021 tactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    43081 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    43141 attaccgagc gcccggactg gcgcgaaaaa gcccacgaat acggtttcaa ttttcacacc
    43201 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    43261 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    43321 gtgatcgcca gcgatgagct gatgaccaaa ttccgcattc caaaacgcac ctggagtttt
    43381 gtgcgtcagt catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcatgg
    43441 gatggcactg gcgaacctaa actgctggaa aataacgccg atacaccaac gtcactatac
    43501 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    43561 gagggcagcg accagtttaa cagtctgcaa gaaaagctaa tcgatcgctt tgtcgagttg
    43621 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    43681 cgcggaacca ttcagtattt gcaggactgc gcgacggaag ctgaaatcgc taccgagttc
    43741 ctctacattg acgatatcgg gttaggtgaa aaaggtcagt tcacggattt acaggatcag
    43801 gtaatttcca acctgttcaa gctgtatcca tgggaattta tgttgcgtga gatgttctcc
    43861 accaagctgg aggatgcagg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    43921 aacaaagcgc ttctaccgct actatgggag atgttcccga atcacccgaa cctgctgccc
    43981 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    44041 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    44101 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    44161 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    44221 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttatt
    44281 gagtaatcaa tgatgccgga tggcattcgc catccggtaa ttgttaacct atctgcaccg
    44341 acagcatact caggctgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    44401 catcccacgc acctaagaca tacaacaacg gcagatagtg ctccggcgtt gggttcgata
    44461 acgcgccacc ttcatggtca aggtaattca ccagaggatg ctgctctacc gccccttgcc
    44521 acgtcagatt cgctttcaca tactcattaa acgacatcgc ccaaggatat ggtgaactat
    44581 caccgtgcca cttcactgta cgcaggttat gcaccacgtt gccactggcg accaacatta
    44641 ttccttcatc tcgcagcgct gccagtttgc gccccatttc gaaatgccag gcggcaggtt
    44701 tgctactgtc gatactcaac tgcaccatcg ggatatcggc gtcaggatac atcttaatca
    44761 gcacgcccca ggaaccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggaccg
    44821 acgctaacag ctcaaccaga tgctgtgcca gcacaggcga acccggagca ggataatgcg
    44881 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    44941 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    45001 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    45061 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    45121 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    45181 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    45241 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    45301 ttattggcgc gtttctcggc gttctcgggc aaaaaccctc gaaccgctta ctggcgtttt
    45361 cgctaggatt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    45421 cactagcagc tgaaggaatg tcgcctgtgt tgggttatgg aatgtttatc ttcggtctgc
    45481 ttggctattt tggcctggac cgcatgttgc cacatgcgca tccgcaggat ttaatgcaaa
    45541 aatcggtgca gccgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    45601 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    45661 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    45721 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    45781 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    45841 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    45901 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    45961 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    46021 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    46081 aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta tttgttccgc
    46141 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    46201 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    46261 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    46321 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    46381 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    46441 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    46501 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    46561 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    46621 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    46681 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    46741 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    46801 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    46861 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    46921 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    46981 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    47041 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    47101 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    47161 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    47221 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    47281 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    47341 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    47401 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    47461 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    47521 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    47581 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    47641 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    47701 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgcggctt gaacaaagaa
    47761 tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca gccagatcca gagactctcc
    47821 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcttctaatt
    47881 tacagtgctt tctattacct tatcgcttaa ttccattcag aatatcatca caaaaatgaa
    47941 tgagtgcaat tgaaaatata attgttatgt attttttaag ttttacttaa tccatattta
    48001 gataaactaa tttcataaaa cccgaaaagt aaaatataat atttgaatga ttagcaatcc
    48061 ttaaataaca gttactaatc attcattttc tacttatatt tatataaagg aatattatgt
    48121 ctgcttttaa aaagtcactt cttgttgctg gcgtagcaat gattctttcc aataacgtat
    48181 ttgctgacga aggtcacggc attgttaaat ttaaaggtga agttatctct gctccatgtt
    48241 caattaaacc aggtgacgaa gacttaactg taaatctggg tgaagtagca gataccgttc
    48301 tgaaaagcga ccagaaatct ttggctgagc ctttcactat tcacctgcaa gactgcatgt
    48361 tgagccaggg tggaaccact tattcaaaag cgaaggttac tttcaccaca gcgaatacta
    48421 tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag
    48481 gcgttggcgt acgtattctg gatagccaga gcggcgaagt gactttaggt actccggtag
    48541 taattacatt caacaatact aacagctatc aggaactgaa cttcaaagct cgtatggaat
    48601 ccccgggcaa agatgctacc ccgggcaacg tttacgctca ggctgactat aagattgctt
    48661 acgagtaatc acattaggcc gggttcgccc ggccacatca ttaacggatt aatgataagt
    48721 ggaacagatg tataaaaaat taaaattaac cacaataagc gaattgatta aaaatattta
    48781 ttgttcatta tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa
    48841 agatttaatc gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg
    48901 ccaattaccc gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat
    48961 ccatctggac ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga
    49021 gcaattaaca ttattaggct ttactgatga gattattgaa gaggctcagc aaaacctgat
    49081 cgatggttgt taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa
    49141 aatgcaatta tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac
    49201 gcctcctgaa ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc
    49261 ctctcattat gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc
    49321 tggggttaat cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa
    49381 caatggcaaa agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat
    49441 cccagcaatt aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga
    49501 ctctttccat ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct
    49561 acgtggttac gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc
    49621 acagaacaac cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa
    49681 tttattcaat acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggccagt
    49741 ctatatatca atgacactaa ccattacatg aattatcaga atgaaaaaga gagttatatt
    49801 gtcacgttta accagtatct tgaatcatta
//
LOCUS       DAJTVK010000016        46684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000016 DAJTVK010000000
VERSION     DAJTVK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.016"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OO372_000972"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="OO372_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6340547.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="OO372_000973"
     CDS             complement(280..873)
                     /locus_tag="OO372_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6340548.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="OO372_000974"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="OO372_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6340549.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="OO372_000975"
     CDS             1788..2183
                     /locus_tag="OO372_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6340550.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="OO372_000976"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="OO372_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6340551.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="OO372_000977"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="OO372_000977"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6340552.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="OO372_000978"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="OO372_000978"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6340553.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="OO372_000979"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="OO372_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6340554.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OO372_000980"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="OO372_000980"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6340555.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OO372_000981"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="OO372_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6340556.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="OO372_000982"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="OO372_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6340557.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="OO372_000983"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="OO372_000983"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6340558.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="OO372_000984"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="OO372_000984"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6340559.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="OO372_000985"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="OO372_000985"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6340560.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="OO372_000986"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="OO372_000986"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6340561.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OO372_000987"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="OO372_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR6340562.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OO372_000988"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="OO372_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6340563.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="OO372_000989"
     CDS             17158..17709
                     /locus_tag="OO372_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6340564.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OO372_000990"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="OO372_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6340565.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="OO372_000991"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="OO372_000991"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6340566.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OO372_000992"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="OO372_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6340567.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="OO372_000993"
     CDS             complement(21354..22376)
                     /locus_tag="OO372_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6340568.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="OO372_000994"
     CDS             complement(22390..23892)
                     /locus_tag="OO372_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6340569.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OO372_000995"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="OO372_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR6340570.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="OO372_000996"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="OO372_000996"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6340571.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="OO372_000997"
     CDS             complement(26207..26548)
                     /locus_tag="OO372_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6340572.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OO372_000998"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="OO372_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6340573.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OO372_000999"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="OO372_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6340574.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="OO372_001000"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="OO372_001000"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6340575.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="OO372_001001"
     CDS             33227..34570
                     /locus_tag="OO372_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6340576.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="OO372_001002"
     CDS             complement(34650..34856)
                     /locus_tag="OO372_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6340577.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="OO372_001003"
     CDS             35181..35735
                     /locus_tag="OO372_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6340578.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /locus_tag="OO372_001004"
     CDS             complement(35825..36736)
                     /locus_tag="OO372_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340579.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OO372_001005"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="OO372_001005"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6340580.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="OO372_001006"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="OO372_001006"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6340581.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="OO372_001007"
     CDS             complement(39939..40319)
                     /locus_tag="OO372_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6340582.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="OO372_001008"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="OO372_001008"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6340583.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="OO372_001009"
     CDS             41376..42341
                     /locus_tag="OO372_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6340584.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="OO372_001010"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="OO372_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6340585.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OO372_001011"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="OO372_001011"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6340586.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OO372_001012"
     CDS             complement(44877..45497)
                     /gene="fklB"
                     /locus_tag="OO372_001012"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6340587.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45716..46354
                     /gene="ytfB"
                     /locus_tag="OO372_001013"
     CDS             45716..46354
                     /gene="ytfB"
                     /locus_tag="OO372_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR6340588.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46338..>46684)
                     /locus_tag="OO372_001014"
     CDS             complement(46338..>46684)
                     /locus_tag="OO372_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6340589.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11455 a        12415 c        11632 g        11182 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgcag
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcagata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    44701 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    44761 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    44821 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    44881 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcgcct
    44941 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    45001 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    45061 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    45121 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    45181 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    45241 ttcacacctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    45301 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    45361 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    45421 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    45481 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    45541 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    45601 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    45661 gttagcttat tcataggcta tgattgagga acaagacgcg gagcaggagg aaaccatgcc
    45721 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    45781 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    45841 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgccggtgg tgacgcgtga
    45901 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc tgcgggcgca
    45961 attagtcaca ccacaaaatg atcctgatca ggtggctccg gtcgctccag aacctataca
    46021 ggaaggccag ccagaagaac agccgcaaac tacccaaacg caaccattcc agccagatag
    46081 cggaattgat aatcaatggc gctcttaccg tgtcgaaccc ggtaaaacga tggcgcagct
    46141 gttccgcgac cacggactgc ccgctaccga tgtctatgca atggcgcagg tagaaggcgc
    46201 gggtaagccg ctgagtaatt tgcaaaatgg gcagatggtg aaaatccgcc agaacgccag
    46261 cggcgtggtg acgggcttaa ccattgatac ggggaataat caacaggtcc tgtttacccg
    46321 ccagccggat ggcagtttta ttcgtgcgcg gtagatgaag ggtcaaccaa aagccagcta
    46381 aggattgtct ctttgatgac cttacgtctg gcttccagtg cctggggtga aaccatactg
    46441 atgtgataaa ccaggccaag cgtatgctgg ttgatcaaat aatatccccc aagcgcagca
    46501 atattaatat tcacctgcaa tgggtcgata tctggcttaa aaatattgtg tttcttccct
    46561 tcatccaata aggattccat taactgcaag tgcgcatggt taatttctaa gagacgcagt
    46621 gattttgcat aatgcacacc tttactttga ttttcactat gcacaatttt taaaaaccaa
    46681 gggt
//
LOCUS       DAJTVK010000017        45926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000017 DAJTVK010000000
VERSION     DAJTVK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.017"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..715)
                     /locus_tag="OO372_001015"
     CDS             complement(<1..715)
                     /locus_tag="OO372_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6340590.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSS"
     gene            complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="OO372_001016"
     CDS             complement(729..1388)
                     /gene="pgmB"
                     /locus_tag="OO372_001016"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6340591.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="OO372_001017"
     CDS             complement(1385..3652)
                     /gene="ycjT"
                     /locus_tag="OO372_001017"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6340592.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3649..4704)
                     /locus_tag="OO372_001018"
     CDS             complement(3649..4704)
                     /locus_tag="OO372_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6340593.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4714..5502)
                     /locus_tag="OO372_001019"
     CDS             complement(4714..5502)
                     /locus_tag="OO372_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR6340594.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5520..6572)
                     /locus_tag="OO372_001020"
     CDS             complement(5520..6572)
                     /locus_tag="OO372_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6340595.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6603..7206)
                     /locus_tag="OO372_001021"
                     /pseudo
     CDS             complement(6603..7206)
                     /locus_tag="OO372_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7193..8074)
                     /locus_tag="OO372_001022"
     CDS             complement(7193..8074)
                     /locus_tag="OO372_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6340596.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8095..9405)
                     /locus_tag="OO372_001023"
     CDS             complement(8095..9405)
                     /locus_tag="OO372_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6340597.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9440..10950)
                     /locus_tag="OO372_001024"
                     /pseudo
     CDS             complement(9440..10950)
                     /locus_tag="OO372_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="OO372_001025"
     CDS             complement(11162..11476)
                     /gene="pspE"
                     /locus_tag="OO372_001025"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6340598.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="OO372_001026"
     CDS             complement(11551..11772)
                     /gene="pspD"
                     /locus_tag="OO372_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6340599.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="OO372_001027"
     CDS             complement(11781..12140)
                     /gene="pspC"
                     /locus_tag="OO372_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6340600.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="OO372_001028"
     CDS             complement(12140..12364)
                     /gene="pspB"
                     /locus_tag="OO372_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6340601.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="OO372_001029"
     CDS             complement(12418..13086)
                     /gene="pspA"
                     /locus_tag="OO372_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6340602.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13253..14230
                     /gene="pspF"
                     /locus_tag="OO372_001030"
     CDS             13253..14230
                     /gene="pspF"
                     /locus_tag="OO372_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6340603.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="OO372_001031"
                     /pseudo
     CDS             complement(14348..15613)
                     /gene="puuE"
                     /locus_tag="OO372_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="OO372_001032"
     CDS             complement(15651..16931)
                     /gene="puuB"
                     /locus_tag="OO372_001032"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6340604.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="OO372_001033"
                     /pseudo
     CDS             complement(16933..18423)
                     /gene="puuC"
                     /locus_tag="OO372_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="OO372_001034"
     CDS             complement(18573..19130)
                     /gene="puuR"
                     /locus_tag="OO372_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6340605.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="OO372_001035"
     CDS             complement(19157..19921)
                     /gene="puuD"
                     /locus_tag="OO372_001035"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6340606.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20133..21494
                     /gene="puuA"
                     /locus_tag="OO372_001036"
     CDS             20133..21494
                     /gene="puuA"
                     /locus_tag="OO372_001036"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6340607.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21643..21807
                     /locus_tag="OO372_001037"
     CDS             21643..21807
                     /locus_tag="OO372_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340608.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21797..23182
                     /gene="puuP"
                     /locus_tag="OO372_001038"
     CDS             21797..23182
                     /gene="puuP"
                     /locus_tag="OO372_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6340609.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23316..23561
                     /locus_tag="OO372_001039"
     CDS             23316..23561
                     /locus_tag="OO372_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6340610.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFLLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23874..25517
                     /gene="sapA"
                     /locus_tag="OO372_001040"
     CDS             23874..25517
                     /gene="sapA"
                     /locus_tag="OO372_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6340611.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25514..26479
                     /gene="sapB"
                     /locus_tag="OO372_001041"
     CDS             25514..26479
                     /gene="sapB"
                     /locus_tag="OO372_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6340612.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26466..27356
                     /gene="sapC"
                     /locus_tag="OO372_001042"
     CDS             26466..27356
                     /gene="sapC"
                     /locus_tag="OO372_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6340613.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27356..28348
                     /gene="sapD"
                     /locus_tag="OO372_001043"
     CDS             27356..28348
                     /gene="sapD"
                     /locus_tag="OO372_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6340614.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28350..29156
                     /gene="sapF"
                     /locus_tag="OO372_001044"
     CDS             28350..29156
                     /gene="sapF"
                     /locus_tag="OO372_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6340615.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29224..29577
                     /locus_tag="OO372_001045"
     CDS             29224..29577
                     /locus_tag="OO372_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6340616.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29945..30733
                     /gene="fabI"
                     /locus_tag="OO372_001046"
     CDS             29945..30733
                     /gene="fabI"
                     /locus_tag="OO372_001046"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6340617.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30878..32004
                     /locus_tag="OO372_001047"
                     /pseudo
     CDS             30878..32004
                     /locus_tag="OO372_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32072..34006
                     /gene="rnb"
                     /locus_tag="OO372_001048"
     CDS             32072..34006
                     /gene="rnb"
                     /locus_tag="OO372_001048"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6340618.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34084..34187)
                     /locus_tag="OO372_001049"
                     /pseudo
     CDS             complement(34084..34187)
                     /locus_tag="OO372_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34241..36231
                     /gene="pdeR"
                     /locus_tag="OO372_001050"
                     /pseudo
     CDS             34241..36231
                     /gene="pdeR"
                     /locus_tag="OO372_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36378..36551
                     /locus_tag="OO372_001051"
     CDS             36378..36551
                     /locus_tag="OO372_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340619.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36641..37390
                     /locus_tag="OO372_001052"
     CDS             36641..37390
                     /locus_tag="OO372_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6340620.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37659..37877
                     /gene="osmB"
                     /locus_tag="OO372_001053"
     CDS             37659..37877
                     /gene="osmB"
                     /locus_tag="OO372_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6340621.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="OO372_001054"
     CDS             complement(38003..38329)
                     /gene="yciH"
                     /locus_tag="OO372_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6340622.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="OO372_001055"
     CDS             complement(38329..39066)
                     /gene="pyrF"
                     /locus_tag="OO372_001055"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6340623.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="OO372_001056"
     CDS             complement(39259..40428)
                     /gene="lapB"
                     /locus_tag="OO372_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6340624.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="OO372_001057"
     CDS             complement(40435..40743)
                     /gene="lapA"
                     /locus_tag="OO372_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6340625.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="OO372_001058"
     CDS             complement(40892..41656)
                     /gene="pgpB"
                     /locus_tag="OO372_001058"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6340626.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41826..42416
                     /gene="ribA"
                     /locus_tag="OO372_001059"
     CDS             41826..42416
                     /gene="ribA"
                     /locus_tag="OO372_001059"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6340627.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="OO372_001060"
     CDS             complement(42480..45155)
                     /gene="acnA"
                     /locus_tag="OO372_001060"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6340628.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45319..45414)
                     /locus_tag="OO372_001061"
     CDS             complement(45319..45414)
                     /locus_tag="OO372_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340629.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45528..45695)
                     /locus_tag="OO372_001062"
     CDS             complement(45528..45695)
                     /locus_tag="OO372_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340630.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45698..45862)
                     /locus_tag="OO372_001063"
     CDS             complement(45698..45862)
                     /locus_tag="OO372_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6340631.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        10847 a        12142 c        11561 g        11376 t
ORIGIN      
        1 ccgatgatcc aataaatccg gcaacaaaca tattcgctgg ttggttataa accgttttcg
       61 gcgcaccaac ttgctgaaca atcccatcct tcataatcac aatccgcgtc gccatggtca
      121 tcgcttcggt ctgatcgtgg gtcacgtaga tcatggtggt attcagcttc tggtgcagct
      181 tgctgatctc tgcgcgcatt tgcacgcgca gcttggcatc aaggttagag agcggttcat
      241 ccattaaaaa cacgcccgct tcgcgcacaa tcgcccgccc gagcgccaca cgctggcgtt
      301 gcccgccgga aagcgcgccc ggcttgcgtt tcaggtactc acgcaggccg agaatttgcg
      361 ccgcccagtt cacccgctca tcaatcacct ctttggcgat tttttgcatc ttcaggccaa
      421 acgccatgtt gtcgtaaacc gtcatatgcg gatacaacgc gtagttctgg aacaccatcg
      481 ctatattgcg tgctttggct ggaacgtcat tcattcgttt gccgtcgatc aacagatcgc
      541 cgccgctgat ctcctcaagc ccggcaatca tgcgcagggt ggtcgactta ccgcagcctg
      601 acggaccgac aaacacgatg aactctttat cggcaatttc caggttgaag tccttcacca
      661 catgcacctg gttatcgtag attttttgaa tatgttgtaa cgaaagctga gccatgttga
      721 ttcctttgct atacgttttg ccagaaggcc gataaccgcg accaggtcag ggagtccgtt
      781 gaaggcaata gtaattgcgc cccggttatg cccgcgccga tccccaccga gcgcataccg
      841 ctggcgttaa tggcgtcaat gcccgcctgc gcatcttcaa tgccgataca tgcctgcggc
      901 ggcacgccca gcccggcaca ggcagcgaga aagatttccg gatccggttt cgagttttta
      961 agttgggaag catccgcgca gaaggtgaaa aactcacgca gctccagtgc atctaaaatc
     1021 gtcggcgcat tgagggagac agaagccagc cctaccgaga tctgttgtgc gcggagatct
     1081 gccagcaaag agcgaatgcc gggtagaaca gcgttgaccg tcaactcgcg caatgagtgg
     1141 acatagagca gatttttgcg atacgccagt tgcgccctct cctgcgagtt aaagtcgccc
     1201 tctttgcccc cgtgttgcag aatgcgccgc agagactcat cgcggctgat cccttttagg
     1261 gattcgttaa actgcgcatc aatgctgatg ccaatttcag cggcaatctg ctgccacgcc
     1321 tggaaatgca gatgcgcggt atcggtgatt acaccatcca gatcgaaaat tacctcttgc
     1381 agtttcattc atcctcctga tgtgtggcag cggtcccatt gaagggcaaa ataaaatcac
     1441 ccaaacagaa aacagattct tcagccacgg atataagctg accgttcaaa cgcagtgaaa
     1501 cgggggcaga agttcgaatc gcaatacgct gcgcgtcaag agtgacctgt aattcgcagc
     1561 cctgccagaa cagagggaaa gacaactgtt gccactgctc aggtaacgcc ggattgagat
     1621 gcaattcacc gtcacgcacg ctccccccgg caaaaccctg aatcgccccc agccagatag
     1681 cgccagttgc ggcagcatgg ataccatcat cacaactatg cggatcagca ccaagatcga
     1741 tttcagtccc ctcgcgccag aactgataac tttgggtcag caggccacag cgtgcggcaa
     1801 caatgccgtg gattgcttta cttaatgacg agtcgtgaat agtgcgcggt tcataaaatt
     1861 gcagattggc aagacacgat gccgctgaga actgctccgg cagcatgtaa ttgagcatca
     1921 ccacatcagc ttgtttgagg atttgcatct cgttcacttc tgcgcgtgaa taatccagca
     1981 gaatggtttg cttccccgcc gccgctttgt atttcgcaag attaatcgcc ggcttagcca
     2041 taaacgaatc atcctgcggc aaaacgccgt cgggctgaat ttctggcagc cgcagttctt
     2101 taaggaacat ttcggcacga tgaataaacg cgtcgtcgct acagccaaac tggcgggcaa
     2161 tactcagcgc ctgctggacg ttgtaatacg ccatatagct ggtgaaggcg ttattattga
     2221 catgttcggt atattcgtca ggcccaataa catcatgaat ttccagacgg tcgttaaccc
     2281 tcaccgcgcg gctaatccag aactttgcag tttccagaag tagcgccatg ccttcatgcg
     2341 caatgaaact ttcatccccc gtggtctgcc agtattgaat aaccgcccag gcgatatcgg
     2401 ccaccagatg atgttccgcc tgcgccgagg ccactttttg ccgcagcccg gtgcgaatgt
     2461 taatggcggc aaattccggc gtctcttctt cgccgctgcg cgcgctttcc cacggaaata
     2521 gcgcgccctg ccagccgttg cgtcgcgctt tctcctgcgc gcctggcaag ttgtgccagc
     2581 gataacgcag taaacttcgg gcaaccgtcg gatcgctaaa cagatgaaac ggtaacaaaa
     2641 atacttctgt atcccagaaa acgtggcctt tgtagccttc gccggttaag ccttttgccg
     2701 caatgctgct gcgctcgtcg tgggcaggcg tcatgatgcg cagatgataa agcgcataat
     2761 ctaacgcttg ctgatcgtgc acttcgccgc catttaccgt gatacgacgt ttctgccacc
     2821 attgacgcca gttttctgtt gatactgcaa gaagttggtc gtaactctgc tgcgcgcaca
     2881 tttcaagctg gcgaagcgac gcgctgcccc actcgtctaa aacggcttgc ctgtcatccc
     2941 gccagtcgat ccacaccagt ttttgcaacg tcactgtctc gcctgcatga agctgcgcac
     3001 tggtatgttg cagtaaacgg cgctctttgg cggtatagca ttgctgcaca tcaccgctca
     3061 ccttacagca acagctgatg gccacatcac tgcgtccatc ctgggtggtg tagatcccct
     3121 gcatcaaatg ctgaccaaac acccgcacct gggtttcgtc gagatgctgg cgaccgtggt
     3181 tggtctgcgt ggcgtcaatg cctgttgaaa tcagcactga cgcgtcggcg tccagtggcg
     3241 taatagtgat ttccagcaca atgagcggca gttggtctgc cgaaacaaag cgacggctgg
     3301 cgatggtgta acctgtgccg ttgctggtac gccagacaac gctacggcgt aattctccac
     3361 tggcaaagtc aagttcacgc tgccaggctt cgcgggataa cgagaaaacc tcaccattta
     3421 tggcaatctc catccccacg acatcaggca ggttcaccag ttcgttgatt tcaccttttc
     3481 ccgcccgatg atacagcccc gccagataca tccctcgcgt ctggcgcgtg taatcttctt
     3541 catggctggc gcgaagccca agatagccgt tcccctgcgc catcagcgat gcatacttgt
     3601 tcagggtatg ctggctgaaa tggggttctg ataacgttac tggcctggtc ataattccac
     3661 acgcgtccct gtttctgcgg attgatacaa cgccgccacc agttgctgga tgatgtaccc
     3721 ctgcttggcg tcggctatca tcacgggctt gccctgtacg tgattgataa aggcttccat
     3781 gctgcgcaaa tggcggttgt cgtctgccat tttccgttgc atcagcgtca ttaattcacc
     3841 gttgttatcg gtgtagatat gtgctggaaa cagcgtcgca ccagctttat caccacaaaa
     3901 gctgacgttc ataatcgact gttcgcggat gttgagtgca aatgacgttt ccagccacag
     3961 aatgccgccg ttatgaaatt caatggtgcc aaacagcgaa tcttcgacgc tgtaagttgc
     4021 cggatcccac tcaccaaatt gaccacagct cttttgcgtg ccgatctttt gaaagctatg
     4081 cgcattcacg cttttcaccg ccggaaagcc cagcacatac atcgcagcat ccagcatatg
     4141 aatgccgatg tcgatcagcg ggccaccacc ctgcagttct ttattggtaa agacacccca
     4201 gccgggaacg ccgcagcgac gcagggcgcg ggcggtggta acgtaaatct ctcccaaaac
     4261 gccgttggtc acctgttcac gcagctgttg cgtatcgagc gcaaaacgat ggtgaaagtc
     4321 gtaggccagc accttgccca gtttgcgcgc ggtatcgcac atttcccgcg cctgttctgg
     4381 cgtcatggcg agcggttttt cgcacatcac atggcagccc gcttccagtg ccatcagggt
     4441 atgttcgtaa tgaaaacggt taggtgagca gacgctaacc acatcaggtt tcaccgccag
     4501 cagcatggcc tgcggatcgt cccacacgga tgcattcccg tatttttctg ccagcgcctg
     4561 cgcctgggaa aggcggctgt cacagacagc caccagttcc agatcgttgc gggtgcagta
     4621 gtacgaagca tgaaccttat ccgccacctg gcctgcgcct attatcgcga cccgcagcgg
     4681 agagcttgtc attgcacttt tcacaaagac ctcttagcag gtacgcaacc aggccaacga
     4741 atcacggtac gcctgggcag gatcttccgc ccggatacgc ccttcgtaca ctacatagcc
     4801 ctgatagtta tccgcgcgca gctgttcaaa cagcgcgtgg aaatccaggg tgccgctgcc
     4861 cggctggtag cgatggttat ccgcaatatg cacatgaccg agcaggtcgc ggttgtcatg
     4921 cagcgcctgc gccaggttat cttcttcgat attcatgtga tagaaatcgc cgataatctg
     4981 tacatgttta agatcgtttt cgacgatgta acggcgggca tcggcgaggg tgttgatcat
     5041 atgatcctga tagcggttta acggttcgag atacaccact gttccggtac gcgcggcgac
     5101 ctgttccagt acgcgcaggg aatcactcac cattttgcgg tcgccgtcca ggctacgcgg
     5161 cgaggtcatc ggcggtaagc ggaaggtaaa catgccccac gctgccggaa cgacgatacc
     5221 tttatccccc acttctgcca gcgcttcgag aatgcgttcg atctgcttta agccattaag
     5281 acgacgctct tcgataaagt cgccaatcca accgtcatag ccaccgcagg cggtggtcac
     5341 cggtaaaccg gtttctttga ttgctgcttt gacttcttcg agattgttga ccagcagttt
     5401 gccgtcaatc tcaaaaccat cgaagcccat ctctttgata taacgaaatt tctccagaat
     5461 gttttccgga aagaatgcct gattctgtgt gcctattttc attttttcat tcctttgaat
     5521 taaaacgtaa cgcccatttt gatgctctgt tccggatgct gatcgacata ctgcatgtag
     5581 ctttccgggc tattggcaaa ggtcactacc gggtcgatta aatcttcgca attgagataa
     5641 ccgttcatca gcagttccca gcaggtttct tcaatacgct tacggctcca gcgcggataa
     5701 tccgggttcg gttcgctgca cgcgcgggag aaaacaattt tggcgttatt gaaatgcgct
     5761 tcgcgtccga ggttaaaacc ttcagcaaat ggtttggcaa acgcaacata ggagatggtg
     5821 ccaccgtagg ccagaccgcg gaacgccgat tgcagcgcgt cggcatagcc gctggtttcg
     5881 ataatcacat cggcaccctg cttgccggtc agcgttttga tctctttgcc tacgtcagta
     5941 ccaatggggt taaggcagaa atccgcgccg tggcgacggg caatatcaca gcgatgagcg
     6001 ataggatcga cgccaatcac gaccgacgcg ccagcacgtt tagccagttg aatggcaatt
     6061 tgaccaatcg cgccaagccc taccaccaca acaaaatcac ccacgcgcac gttggcatcg
     6121 cgcacgccgc tcatggcaaa ctgcgccggg tcgtaacaaa cggcgttttt ccacgagctg
     6181 ccttccggca ttttgcgcag cttgtagtta ttcaccgcgt taatgatgac cgtctcggag
     6241 agcgggccgt agccgcatac gctgtcgccc accgcgtagt cggtaacgtc gctgccgcac
     6301 tcgataatgt cgccaaccac catgtttcca agctggaatt tgccaaactc aatgccgcgc
     6361 ggcgcatctg ccggacgcgg agtgaacatc tgccattcgc cgttaaagtc ttcatcgata
     6421 aacgggctgg cggcgcggaa gtcgaccact tccgttccgt gtttcggtgc gccgaaacgc
     6481 acgcggacct tcacttcatt agctaaaatc gcccgatctt catactcaac cagcgcagca
     6541 acacgcggtg ctgtggctac taactttttc atttaaaact ccttgtaaag aaactggccg
     6601 ctttagccct tcacgccacc ggcggtcaaa ccactcttaa taaaacgttc cgaaagcgca
     6661 tacataatca ccaccggcaa tgcggtcacc agtgaggccg ccatcatccg tccccagatg
     6721 taatctggcg tactgaacag cgcgttcagg cccaccggta aggtgaaatt gccggcgctg
     6781 gagaggaaaa tcgacgcaaa cagatagtcg ttccacgcca ccataaagca gtagacgaac
     6841 acggatatca gcccggacat cgccaacggc acggtgatgc ggaagataat ttgcagacgg
     6901 ttgaggccat ccatcatcgc cgcttcttcg atctcatcgg ggatggtgtc gaagtagctt
     6961 ttcagcataa acacggcggt gggcagagtt tgcgtcacca tggtgatgat cagcgccatc
     7021 tcagtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7081 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7141 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7201 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7261 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7321 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7381 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7441 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7501 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7561 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7621 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7681 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7741 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     7801 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     7861 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     7921 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     7981 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8041 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8101 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8161 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8221 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8281 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8341 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8401 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8461 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8521 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8581 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8641 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8701 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     8761 gttaaagaca ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     8821 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     8881 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     8941 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9001 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9061 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9121 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9181 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9241 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9301 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9361 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9421 atttaatcca cataacctgt taggggcgca gcgttatttc attacccaat agatcaactt
     9481 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9541 cattaaacaa tccagtcaga caatcaccat cagcgttact tctttgaata cgcattacgg
     9601 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9661 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9721 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
     9781 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
     9841 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
     9901 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
     9961 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10021 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10081 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10141 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10201 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10261 ggcatccagg cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10321 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10381 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10441 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10501 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10561 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10621 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10681 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10741 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    10801 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    10861 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    10921 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    10981 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11041 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11101 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11161 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11221 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11281 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11341 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11401 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11461 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11521 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11581 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11641 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11701 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    11761 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    11821 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    11881 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    11941 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12001 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12061 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12121 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12181 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12241 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12301 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12361 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12421 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12481 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12541 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12601 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12661 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12721 aatctctttc ttcatgcgtg ccagcgtatc gttcaccagc gtcacttcat gttccaggga
    12781 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    12841 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    12901 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    12961 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13021 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13081 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13141 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13201 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13261 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13321 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13381 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13441 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13501 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13561 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13621 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13681 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13741 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    13801 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    13861 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    13921 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    13981 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14041 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14101 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14161 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14221 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14281 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14341 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14401 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14461 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14521 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14581 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14641 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14701 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    14761 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    14821 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    14881 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    14941 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15001 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15061 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15121 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15181 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15241 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15301 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15361 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15421 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15481 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15541 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15601 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15661 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15721 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    15781 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    15841 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    15901 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    15961 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16021 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16081 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16141 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16201 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16261 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16321 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16381 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16441 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16501 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16561 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16621 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16681 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16741 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    16801 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    16861 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    16921 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    16981 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17041 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17101 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17161 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17221 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17281 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17341 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17401 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17461 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17521 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17581 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17641 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17701 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    17761 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    17821 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    17881 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    17941 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18001 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18061 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18121 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18181 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18241 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct aggtgaccgg
    18301 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18361 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18421 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18481 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18541 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18601 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18661 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18721 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    18781 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    18841 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    18901 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    18961 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19021 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19081 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19141 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19201 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19261 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19321 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19381 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19441 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19501 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19561 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19621 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19681 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19741 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    19801 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    19861 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    19921 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    19981 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20041 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20101 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20161 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20221 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20281 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20341 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20401 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20461 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20521 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20581 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20641 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20701 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    20761 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    20821 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    20881 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    20941 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21001 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21061 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21121 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21181 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21241 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21301 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21361 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21421 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21481 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21541 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21601 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21661 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21721 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    21781 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    21841 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    21901 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    21961 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22021 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22081 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22141 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22201 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22261 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22321 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22381 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22441 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22501 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22561 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22621 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22681 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22741 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    22801 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    22861 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    22921 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    22981 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23041 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23101 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23161 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23221 cgcgccgcgc cattaactat aaaaagatgt ataacccgga tcacgagtgc tatcatccgc
    23281 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23341 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23401 gcacaccgct ggcccactac ttcctgttac tgctcacccg tttgatgaac aatgaagaga
    23461 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23521 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23581 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23641 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23701 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    23761 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    23821 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    23881 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    23941 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24001 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24061 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24121 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24181 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24241 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24301 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24361 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24421 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24481 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24541 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24601 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24661 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24721 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    24781 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    24841 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    24901 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    24961 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25021 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25081 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25141 cgcggttgat ggatatgagc catgatctga cattatccgg ttgggcgacg gacagtaacg
    25201 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25261 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25321 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25381 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25441 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25501 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25561 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25621 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25681 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25741 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    25801 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    25861 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    25921 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    25981 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26041 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26101 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26161 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26221 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26281 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26341 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26401 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26461 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26521 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26581 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26641 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26701 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    26761 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    26821 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    26881 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    26941 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27001 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27061 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27121 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27181 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27241 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27301 tggttaacct ccttggcgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27361 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27421 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27481 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27541 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27601 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27661 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27721 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    27781 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    27841 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    27901 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    27961 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28021 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28081 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28141 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28201 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28261 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28321 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28381 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28441 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28501 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28561 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28621 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28681 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28741 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    28801 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    28861 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    28921 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    28981 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29041 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29101 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29161 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29221 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29281 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29341 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29401 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29461 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29521 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29581 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29641 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29701 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    29761 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    29821 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    29881 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    29941 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30001 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30061 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30121 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30181 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30241 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30301 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30361 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30421 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30481 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30541 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30601 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30661 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30721 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    30781 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    30841 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    30901 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    30961 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31021 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31081 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31141 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31201 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31261 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31321 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31381 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31441 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31501 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31561 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31621 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31681 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31741 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    31801 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    31861 gcacccgatc gctttagcgc cgcccagttc agccctttac tcggacacga aaaatcatca
    31921 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    31981 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32041 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32101 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32161 aaaaggcttt ggcttcctgg aagtcgacgc gaaaaaaagt tatttcattc cgccgccgca
    32221 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32281 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32341 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32401 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32461 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32521 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32581 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32641 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32701 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    32761 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    32821 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    32881 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    32941 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33001 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33061 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33121 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33181 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33241 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33301 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33361 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33421 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33481 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33541 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33601 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33661 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33721 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    33781 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    33841 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    33901 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    33961 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34021 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34081 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34141 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34201 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34261 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34321 cgatgttttg cgctttttta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34381 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34441 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34501 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34561 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34621 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34681 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34741 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    34801 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    34861 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    34921 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    34981 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc tgataacaat
    35041 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35101 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35161 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35221 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35281 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35341 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35401 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35461 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35521 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35581 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35641 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35701 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    35761 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    35821 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    35881 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    35941 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36001 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36061 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36121 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36181 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36241 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36301 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36361 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36421 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36481 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36541 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36601 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36661 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36721 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    36781 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    36841 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    36901 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    36961 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37021 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37081 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37141 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37201 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37261 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37321 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37381 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37441 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37501 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37561 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37621 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37681 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37741 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    37801 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    37861 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    37921 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    37981 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38041 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38101 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38161 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38221 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38281 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38341 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38401 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38461 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38521 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38581 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38641 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38701 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    38761 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    38821 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    38881 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    38941 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39001 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39061 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39121 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39181 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39241 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39301 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39361 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39421 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39481 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39541 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39601 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39661 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39721 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    39781 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    39841 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    39901 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    39961 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40021 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40081 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40141 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40201 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40261 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40321 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40381 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40441 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40501 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40561 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40621 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40681 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40741 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    40801 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    40861 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    40921 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    40981 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41041 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41101 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41161 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41221 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41281 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41341 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41401 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcaaaaa cgcagacacc
    41461 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41521 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41581 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41641 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41701 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    41761 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    41821 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    41881 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    41941 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42001 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42061 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42121 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42181 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42241 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42301 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42361 acgaacatta tctcgatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42421 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42481 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42541 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42601 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42661 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42721 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    42781 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    42841 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    42901 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    42961 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43021 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43081 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43141 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43201 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43261 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43321 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43381 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43441 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43501 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43561 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43621 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43681 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43741 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    43801 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    43861 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    43921 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    43981 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44041 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44101 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44161 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44221 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44281 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44341 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44401 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44461 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44521 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44581 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44641 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44701 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    44761 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    44821 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    44881 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    44941 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45001 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45061 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45121 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45181 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45241 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45301 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45361 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45421 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45481 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45541 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45601 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45661 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45721 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    45781 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    45841 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    45901 caaatcctaa agaaacttta gtcaaa
//
LOCUS       DAJTVK010000018        44873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000018 DAJTVK010000000
VERSION     DAJTVK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.018"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2329
                     /gene="torZ"
                     /locus_tag="OO372_001064"
     CDS             <1..2329
                     /gene="torZ"
                     /locus_tag="OO372_001064"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6340632.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
     gene            complement(2494..3465)
                     /gene="cmoB"
                     /locus_tag="OO372_001065"
     CDS             complement(2494..3465)
                     /gene="cmoB"
                     /locus_tag="OO372_001065"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6340633.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3462..4205)
                     /gene="cmoA"
                     /locus_tag="OO372_001066"
     CDS             complement(3462..4205)
                     /gene="cmoA"
                     /locus_tag="OO372_001066"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6340634.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(4246..4641)
                     /locus_tag="OO372_001067"
     CDS             complement(4246..4641)
                     /locus_tag="OO372_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6340635.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(4694..4933)
                     /locus_tag="OO372_001068"
                     /pseudo
     CDS             complement(4694..4933)
                     /locus_tag="OO372_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5183..5713)
                     /locus_tag="OO372_001069"
     CDS             complement(5183..5713)
                     /locus_tag="OO372_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340636.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            5904..6152
                     /locus_tag="OO372_001070"
     CDS             5904..6152
                     /locus_tag="OO372_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6340637.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            6154..8244
                     /locus_tag="OO372_001071"
     CDS             6154..8244
                     /locus_tag="OO372_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6340638.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            8327..9259
                     /locus_tag="OO372_001072"
     CDS             8327..9259
                     /locus_tag="OO372_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6340639.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIAMAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            9262..9483
                     /locus_tag="OO372_001073"
     CDS             9262..9483
                     /locus_tag="OO372_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340640.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            9496..9750
                     /locus_tag="OO372_001074"
     CDS             9496..9750
                     /locus_tag="OO372_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340641.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            9752..10033
                     /locus_tag="OO372_001075"
     CDS             9752..10033
                     /locus_tag="OO372_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HCR6340642.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            10030..10302
                     /locus_tag="OO372_001076"
     CDS             10030..10302
                     /locus_tag="OO372_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340643.1"
                     /translation="MSKVVRIIFEYKEDVICKNSDGSTHRGVSLDIRSTGIKKKDNGP
                     AMIFGVVMLAESRNFAEFVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            10307..10600
                     /locus_tag="OO372_001077"
     CDS             10307..10600
                     /locus_tag="OO372_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340644.1"
                     /translation="MSKVRVIFEFNHVSHDEKLAGNDCVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPAIIQFLSAEFQASAQSFGADAIIKRHRVHKASGTLQ"
     gene            10612..11142
                     /locus_tag="OO372_001078"
     CDS             10612..11142
                     /locus_tag="OO372_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HCR6340645.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKQIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            11288..11821
                     /locus_tag="OO372_001079"
     CDS             11288..11821
                     /locus_tag="OO372_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340646.1"
                     /translation="MIDAKVLEGVKNWLCIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCGFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            11821..12336
                     /locus_tag="OO372_001080"
     CDS             11821..12336
                     /locus_tag="OO372_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340647.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFYFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            12340..12891
                     /locus_tag="OO372_001081"
     CDS             12340..12891
                     /locus_tag="OO372_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb domain-containing protein"
                     /protein_id="HCR6340648.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTHISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            12888..13217
                     /locus_tag="OO372_001082"
     CDS             12888..13217
                     /locus_tag="OO372_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340649.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAAVLIVQYGHRQELRIGHMGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREVNA"
     gene            13214..13564
                     /locus_tag="OO372_001083"
     CDS             13214..13564
                     /locus_tag="OO372_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340650.1"
                     /translation="MRSYIDNEKLETISDCLSLLAKIKETIEEIKFQLEYAPCGDDAW
                     RNAARKALAVFQKQRRAVEYRLAVLRQEEKERNIRRHERVNDFLVRELKERVPESVFF
                     ECEAIARSKALETD"
     gene            13580..13888
                     /locus_tag="OO372_001084"
     CDS             13580..13888
                     /locus_tag="OO372_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340651.1"
                     /translation="MSIELRSSYEYRKILIAGGMKPEDAEKFVSFMDKECDKRDMPEI
                     IMDDMILDSAVALSPLWIVHTLAEIAKRSDKQAAVAALRLLAELSCSVNPNTFFDEPG
                     "
     gene            complement(13850..14260)
                     /locus_tag="OO372_001085"
     CDS             complement(13850..14260)
                     /locus_tag="OO372_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCR6340652.1"
                     /translation="MKIDYDEVLSILTTFQDAETPFLTLQDLGMAEAEGEEKDKKVFH
                     LMLLADNGSIVNGDMQSETPKYIGFFFHSLGVGFRNTPIMLTQQGHDLANALRKKPIL
                     ERIKKEFTDAPFDLIKEVTKSMLTRFVKERIGID"
     gene            14325..14837
                     /locus_tag="OO372_001086"
     CDS             14325..14837
                     /locus_tag="OO372_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HCR6340653.1"
                     /translation="MNRASLITLIHVAKRDLQLDHETYTSVLLAATGKPSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            14908..15330
                     /locus_tag="OO372_001087"
     CDS             14908..15330
                     /locus_tag="OO372_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6340654.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            15402..15902
                     /locus_tag="OO372_001088"
     CDS             15402..15902
                     /locus_tag="OO372_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6340655.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            15937..16365
                     /locus_tag="OO372_001089"
     CDS             15937..16365
                     /locus_tag="OO372_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340656.1"
                     /translation="MCRCAAAGCAVSAVNNPLPALIAGGCMAALGVMSVLAAVVWGMH
                     QKTQRLEDNNHVLVRERDEARLVLANQQRTLQLISQISKAATNEKQQNMQHSEEQQSI
                     VRRSLATVPAAAVPVPDDVADRVRRAVCEIRAGEAGADPR"
     gene            16349..16567
                     /locus_tag="OO372_001090"
     CDS             16349..16567
                     /locus_tag="OO372_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340657.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            16578..16805
                     /locus_tag="OO372_001091"
     CDS             16578..16805
                     /locus_tag="OO372_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCR6340658.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            16786..17097
                     /locus_tag="OO372_001092"
     CDS             16786..17097
                     /locus_tag="OO372_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HCR6340659.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            17090..17380
                     /locus_tag="OO372_001093"
     CDS             17090..17380
                     /locus_tag="OO372_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6340660.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            17383..17964
                     /locus_tag="OO372_001094"
     CDS             17383..17964
                     /locus_tag="OO372_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HCR6340661.1"
                     /translation="MDKPARGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            17975..18223
                     /locus_tag="OO372_001095"
     CDS             17975..18223
                     /locus_tag="OO372_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340662.1"
                     /translation="MATDNRSLMSSAHQKAFSLATQLPPETIKVCRELTELSIAGEIS
                     LEQLHSLLAEAATDELSGVDVMTEELESRIKSILTGKA"
     gene            18223..19887
                     /locus_tag="OO372_001096"
     CDS             18223..19887
                     /locus_tag="OO372_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340663.1"
                     /translation="MNKARETSPSLMQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDNFISRAAWLRESEVLTWCEEH
                     LKPLLEKLNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALQERSRAMEGGYRDDDEAYHEFDRGGW"
     gene            19887..21476
                     /locus_tag="OO372_001097"
     CDS             19887..21476
                     /locus_tag="OO372_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HCR6340664.1"
                     /translation="MGRIIDTDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHSGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTTHTIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDRETPPEKQEKTEQTALSARLPEAKS
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLADALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDDTGD"
     gene            21460..22791
                     /locus_tag="OO372_001098"
     CDS             21460..22791
                     /locus_tag="OO372_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HCR6340665.1"
                     /translation="MTQGIDLGYAATLPSKEAVAYFRVKGAHISWNWFETAADVHARS
                     FTAAKAARLDVVTTLQAEVQRAIDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALMPEDFGRLPAMLAKPKAVLWDKRHKNLMYIVENKDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(22793..22872)
                     /locus_tag="OO372_001099"
     tRNA            complement(22793..22872)
                     /locus_tag="OO372_001099"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            22913..23386
                     /locus_tag="OO372_001100"
     CDS             22913..23386
                     /locus_tag="OO372_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR6340666.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            23564..24688
                     /locus_tag="OO372_001101"
     CDS             23564..24688
                     /locus_tag="OO372_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HCR6340667.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINHAAVERMVSRVVALNQPVKIDYNHQTLIKGHPAPAAGFVMASPENFRFS
                     EERGFEVRPDWNPPAIEHLRNNEFPWFSPVIGYDEITGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTLQTD
                     TVTVPDKKTATAALSAEDIAAAHLLGKTEAEFLKMKEEMQ"
     gene            24688..25635
                     /locus_tag="OO372_001102"
     CDS             24688..25635
                     /locus_tag="OO372_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HCR6340668.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNTTVSNEMKGKLELVVADYL"
     gene            25679..26032
                     /locus_tag="OO372_001103"
     CDS             25679..26032
                     /locus_tag="OO372_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340669.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRAQFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAGQDAPR
                     KKTGNNARQAKKARA"
     gene            26029..26448
                     /locus_tag="OO372_001104"
     CDS             26029..26448
                     /locus_tag="OO372_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HCR6340670.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDARKLNRALTDASA
                     LIDSYLSARYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            26445..27008
                     /locus_tag="OO372_001105"
     CDS             26445..27008
                     /locus_tag="OO372_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HCR6340671.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLISAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHISLPGATAAGEKAGPSEE"
     gene            27012..27299
                     /locus_tag="OO372_001106"
     CDS             27012..27299
                     /locus_tag="OO372_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6340672.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMKPLAQEGEEKPFTPFWCRRLNDGD
                     VIVVEKAAEAAPAAASATTDAAGKPAGAAAEKPATEKPATEKPAASDKGTS"
     gene            27299..28801
                     /locus_tag="OO372_001107"
     CDS             27299..28801
                     /locus_tag="OO372_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6340673.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGVGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPARLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADNGTPVRPGQNIVTPEIMTLQLIALGEEWV
                     ELGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            28810..29175
                     /locus_tag="OO372_001108"
     CDS             28810..29175
                     /locus_tag="OO372_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6340674.1"
                     /translation="MSGKQHQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            29190..29669
                     /locus_tag="OO372_001109"
     CDS             29190..29669
                     /locus_tag="OO372_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6340675.1"
                     /translation="MTTRKKKTAVSDEAVMEAIRDVLEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAETEAERLMETRNGGPVLIASPALRGIALLRR
                     QIAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            29797..31854
                     /locus_tag="OO372_001110"
     CDS             29797..31854
                     /locus_tag="OO372_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6340676.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSEAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFARDVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            31841..33199
                     /locus_tag="OO372_001111"
     CDS             31841..33199
                     /locus_tag="OO372_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HCR6340677.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPAVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAGTPSGSLTTNRDSMAEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTLGKLLDQVIRQNTTHVQDTDR
                     SGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGYTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSSRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            33183..34307
                     /locus_tag="OO372_001112"
     CDS             33183..34307
                     /locus_tag="OO372_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6340678.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRSKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            34297..34911
                     /locus_tag="OO372_001113"
     CDS             34297..34911
                     /locus_tag="OO372_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR6340679.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     VLDGTGKSDGLFTMSDAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            34904..35341
                     /locus_tag="OO372_001114"
     CDS             34904..35341
                     /locus_tag="OO372_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6340680.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLSREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            35341..36423
                     /locus_tag="OO372_001115"
     CDS             35341..36423
                     /locus_tag="OO372_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6340681.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTLNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            36414..36974
                     /locus_tag="OO372_001116"
     CDS             36414..36974
                     /locus_tag="OO372_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR6340682.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            36974..37855
                     /locus_tag="OO372_001117"
     CDS             36974..37855
                     /locus_tag="OO372_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6340683.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNRAANALPSDGTAVAANRLANAHTINGVPFDGTQD
                     ITISSGTVTAIRLGSVTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYY
                     RPLQYLLNGAWVTAASI"
     gene            complement(37887..38408)
                     /locus_tag="OO372_001118"
     CDS             complement(37887..38408)
                     /locus_tag="OO372_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6340684.1"
                     /translation="MELKNITRYYPENMPYGNNVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGVICSISQDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYAR
                     KLEAMRQSYLNQAYEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTE
                     AEFNAIKWPEQPQ"
     gene            complement(38460..38783)
                     /locus_tag="OO372_001119"
     CDS             complement(38460..38783)
                     /locus_tag="OO372_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6340685.1"
                     /translation="MPSDGTAVAANRLATPRNINGVPFDGTQDINITSGITQQDADSR
                     YIQDIRLGAENRQVMYGGAYYSAYGNVMAGLQIDSKVDASNDFIAFRPLQKLVGGTWI
                     TVSQL"
     gene            38899..39471
                     /locus_tag="OO372_001120"
     CDS             38899..39471
                     /locus_tag="OO372_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6340686.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGCHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPASPAPSPKVP"
     gene            39557..41611
                     /locus_tag="OO372_001121"
     CDS             39557..41611
                     /locus_tag="OO372_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6340687.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSIEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVN
                     NA"
     gene            41756..41938
                     /locus_tag="OO372_001122"
     CDS             41756..41938
                     /locus_tag="OO372_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR6340688.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQVTPLPKDPTQP"
     gene            41974..42228
                     /locus_tag="OO372_001123"
     CDS             41974..42228
                     /locus_tag="OO372_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR6340689.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAVAASDADNEPKTDAQPEQPDPAQPGTDSTMM"
     gene            complement(42396..42656)
                     /locus_tag="OO372_001124"
     CDS             complement(42396..42656)
                     /locus_tag="OO372_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340690.1"
                     /translation="MIENAEIYNDYFSDYSDACKYSSSAGEFICFNFFIKSYVFPPQQ
                     EDGGVPPPEITLHRIASVSMMEQQANELYSILGTILQKRESD"
     gene            42946..43146
                     /locus_tag="OO372_001125"
     CDS             42946..43146
                     /locus_tag="OO372_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6340691.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQKASYIPYHQHA"
     gene            43100..43837
                     /locus_tag="OO372_001126"
     CDS             43100..43837
                     /locus_tag="OO372_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HCR6340692.1"
                     /translation="MGNRKQVTSRIISTPELIHYNDNIVGYGSRELRVETISCWLARL
                     VVVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNNGARVVTGTQNREYMELN
                     RLWLHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHETTFYELDGEWYHEICKNAIKRGGQRGEHLRTNIDRASVHKFRQFRYIRFLDKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            44666..>44873
                     /locus_tag="OO372_001127"
     CDS             44666..>44873
                     /locus_tag="OO372_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6340693.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAV"
BASE COUNT        11029 a        11191 c        12616 g        10037 t
ORIGIN      
        1 ggacggtaaa atcctcaccg cggaacgttg gggagcgatg aacgtagaag tgaaggacgg
       61 caagattgtt tcttcaacag gcgcgctggc gaaaaccata ccgaattcct tacagtctac
      121 ggcggcggat caggtacaca ccacgacgcg tattcagcat ccgatggtga gaaaaagcta
      181 tctcgataat ccactgcaac cgacgaaagg tcgtggcgaa gatacctatg tacaggtgag
      241 ctgggaacaa gcattaaaac tgattcatga gcaacacgat cgcattcgca aagctaacgg
      301 accatcggct atttttgccg gatcatacgg ctggcgctct tctggcgtgt tgcataaagc
      361 gcaaacctta ctgcaacgtt atatgaacct ggcgggcggt tattccgggc atagcggcga
      421 ttattcaacc ggggcggcac aggtgatcat gccgcatgtg gtcggttctg tagaggtgta
      481 tgaacagcag acctcctggc cgctgattct ggaaaacagc caggttgtgg tgctgtgggg
      541 gatgaacccg ctgaatacct taaaaattgc ctggagcagt accgatgagc aagggctgga
      601 atacttccat cagctgaaaa aatctggcaa accagtgatt gccattgatc ctatccgctc
      661 cgaaaccatc gacttctttg gcgataatgc cacctggatc gcgccgaata tgggcaccga
      721 cgtggcactg atgttaggga ttgcgcatac cctgatgaca caaggcaaac acgataaagt
      781 gttccttgaa aaatacacta ccgggtatcc gcagtttgaa gagtatctga gaggtaagag
      841 cgataatacg ccgaagagcg cggcctgggc ggcggaaatt actggcgtac cggaagcgca
      901 gatagtcaaa ctcgctgaac tgatggcggc gaatcgcaca atgctgatgg caggctgggg
      961 aattcagcgc cagcaatatg gtgaacagaa acactggatg ctggtgacgc tggcggcgat
     1021 gttagggcag attggtacac cgggcggcgg ttttggtttc tcttatcatt actccaatgg
     1081 tggcaacccg acacgtgttg gcggcgtgct gccggaaatg tctgcggcga ttgccggaca
     1141 ggccagcgag gccgctgatg atggcggaat gagggcgatt cctgttgccc gtatcgtcga
     1201 tgcgctggaa aatcctggcg gcaaatatca gcataacggt aaagagcaaa cgtatccgaa
     1261 tatcaaaatg atctggtggg cgggtggcgg taactttacc catcaccagg atactaatcg
     1321 tctgattaaa gcatggcaga aaccggagat gatcgtcgtt tctgaatgct actggaccgc
     1381 agctgctaaa cacgcagata tcgtattacc gatcaccaca tcgtttgagc gcaatgactt
     1441 gacgatgacc ggtgattaca gcaaccagca tattgtgccg atgaagcagg ctgttgctcc
     1501 gcaatttgaa gcgcgaaacg attttgatgt gtttgccgat cttgcggaat tactcaaacc
     1561 tggcggaaaa gagatctata ccgaaggtaa agatgaaatg gcgtggctga aatttttcta
     1621 tgatgccgct cagaaagggg cccgtgcgca acgcgtcact atgccaatgt ttaatgcctt
     1681 ctggcagcaa aataaactga tcgaaatgcg ccgcagcgag aagaacgaac agtacgttcg
     1741 ttatggtgat ttccgcgccg atccggtgaa aaatgcgctg ggtacgccaa gtggcaaaat
     1801 tgagatttac tccaaaacgc tggaaaaatt tggctataag gattgcccgg cacacccaac
     1861 ctggcttgcg cctgatgagt ggaagggtac cgccgacgag aagcagttgc agcttctgac
     1921 cgcacatccg gcacaccgtt tacacagtca gcttaactat gcggaactgc gtaaaaaata
     1981 tgcggttgcc gatcgtgaac cgatcactat tcacaccgaa gatgctgctc gatttagtat
     2041 tgcgaatggc gatctggtgc gtgtctggaa caaacgtggt cagattttga caggcgcggt
     2101 ggtgactgac gggatcaaaa aaggcgtggt atgcgtgcat gaaggtgcat ggccagatct
     2161 ggaaaacggc ttgtgtaaaa acggcagtgc gaacgtgtta acggcggata tccccagctc
     2221 gcagctggca aatgcctgtg ccggtaactc tgcgctggtg tatatcgaaa aatatacggg
     2281 taacgcaccg aagttaacgg catttgataa gcctgctgtt caggcgtaag aagtgaatag
     2341 cgcccggaaa cgggcgctga ggttatgcaa acgtaaaact gcctgatgcg ctatgcttat
     2401 caggcctaca tagtccctgc aatagattga attagcaagg gtttgtaggc cggataaggc
     2461 attcacgccg catccggcag taataccaga cctttacggc ttgcgcgcaa tcagcaccgc
     2521 gcgtttaggc gcaggataac cttcaactgt cttactcgga tcatgtgggt cgaggaaatc
     2581 ggacagagat tcggtaacca tccattcagt gcgtcgctgc tcttcagtag tggtaacgca
     2641 cacatccaca atgcggatat ctacaaaacc acacttcttc agccagtttt tcagcgccag
     2701 cgcggaaggg atgaaataga cattacgcat ttgcgcgtaa cgatcgcctg gtaccagcac
     2761 cgtgttctca tcgccatcaa taaccagcgt ttccagcacc agttcaccct cattcaccag
     2821 ttgatctttc aactgccaga gatgctccag cggcgaacgg cgatggtaaa gcacgcccat
     2881 cgaaaagacg gtatcaaagg ctttcagtgc cggaagctgt tcaataccta acggtaacaa
     2941 atgtgcgcgc tgatcgttac ccagtagttt acgcaccgct tcaaactggc acagaaacag
     3001 ttgcgtaggg tcgatcccca ccgcgaggtg cgcgcctgcg ccaatcatgc gccacatgtg
     3061 ataaccgctg ccgcagccga catccagaat ggtgcgtccg gttaaatcag aaagatgggg
     3121 cagaacacga tcccatttcc agtcggaacg ccattcggta tcgatgttga cgccatacag
     3181 tgagaacggc cctttgcgcc atggcatcag gttccgcata agcgtttcaa tgcgtttaat
     3241 ttgcccggtg ctcagtggct cttcgctttc ggcggttacg ctatgcaata aatccagacg
     3301 atacggttta atttcaggca gaaattccac cgcgttggac cactgcttaa acagcccgtg
     3361 ctgctgctcg cgctgccagt tagcaatctg cgcgggcagc gtttcgagcc agtgtgaaag
     3421 atgatttttg gcaatcagag aataaaagtt accaaagtcg atcatgcagc gtcctctgct
     3481 tttaatgcca ccagtgaacc aaagttaaag cactggaacc acagctcgct atgctcaaaa
     3541 ccggctttat gcaggcgtgc tttatgggtt tccacggaat cggtcagcat cacgttttcc
     3601 agcatgctgc gcttctggct gatctccagt tcgctgtaac cgttggcacg tttaaagtcg
     3661 tggtgcatgt tgaacaacag ttcaccaact ttggcatctt cgaaactgaa tttttccgaa
     3721 agcaccagcg cgccgcccgg gttcagtcct tgataaattt tatccagtaa tgcctggcgc
     3781 tcggaaggtt ccaggaattg cagggtaaaa ttcagcacca ccatcgatgc gttttcaatg
     3841 gcgatatcgc gaatatcacc ttcaatgacg tctactggcg taggggcttt ataggcgtca
     3901 atatgacgac tgcagcgttc aatcatcgcc ggggagttgt cgatggcaat aattttgcaa
     3961 ttatcatgat gaatgttgcg acgcaccgag agcgtcgccg cgcccagaga acaacccaga
     4021 tcgtaaacct gcgtaccagg ttgaacgaag cgctcggcta acataccaat catggaaata
     4081 atattggaat agccgggaac ggaacgctgg atcatatccg ggaagacttc agctacccgt
     4141 tcatcaaagg tccagtcgcc cagtctggcg ataggggcag aaaatagcgt gtcgcggtga
     4201 gacataacgt aaaaatccgg gaaaaagaaa gtggcgtatt gtgcgctaac gcagggagaa
     4261 aaccaactcc cagggcatat accaaagatt cgccagcacc atcagcaaca gcgcacacca
     4321 ggtggcgctc atgccggaac gtcgccagcg aaacagacgg tgatgaaaac cgtaataatg
     4381 catcagacga ccagcaagca aaacgatgcc gcaaatatgc accatccagg tttctgcgcc
     4441 attcatttcc ataaacagca tcagcacaat cgcgatggga atatattcca ccgcgttacc
     4501 atgaatgcga atagcgcttt gcagttcgct aaaaccaccg tcaccatagg caacgcggta
     4561 ctgcattcgc aggcgaacga catcaaaaga gaacttcatt aataacaacg cacttaaaac
     4621 ggcatatagc gcgcttacca tacaaactcc ctttaaaatg gccgatggca cctgtctatg
     4681 ataggtggca aattcagaaa agagaagatt gctgtggaat agccggaact gctccgatct
     4741 ctggaagcgt tttacgtaag tcttcccaca gggtatgtac cagttccggg gcctgggcaa
     4801 tgtctggcgt atgtaaaaaa agataaggcg tagtggtctg atgccactgc gctaatttct
     4861 gtaaccagac ctgaaataat tcccggtttt gcgtcatatc atcactaccg ataaaacgaa
     4921 tcagcggatt tgtgtaacca ggtgaatcat ttgataccaa cgagggtttt agtgaaagga
     4981 ggtgaaatca cctgtaagat actgaaataa aacaggtgat gtctggatga gcgcttaaag
     5041 tctatttgat atcgattgtg caataattaa tcgtttattc taaaatcggc aaaacttttg
     5101 atactgcgat ctcaactttt taaagcgttt aaaacttcat taaatctata tggcaactaa
     5161 catacgggcc agcctcgcca aactaacccg ctttagattc taccggattt gattgcctac
     5221 ttgtctcggt tttttccaaa atctctctga cgcaatcgtc tggcatatct acaatcatca
     5281 acgcagccag gcgcttcctg ccttctagtt gtaataaatc ctggttttct ttattcagca
     5341 gtgtcagaac cgtctctaca cctttgcgag caaggagatt ggttaactgt tctcgttcgt
     5401 ctttcgtaag gaatgaaagc atctgtgtaa gctgatctcc cttatcacca gaacaaccaa
     5461 aattaccata aataccaata tcgtttggta attcaccctt gccctcttta agccactcaa
     5521 gactaacgcc ttcactttca gaaattcgta taaggatact ttctctgggg gctggttgtg
     5581 tttgtcctcc cttgaaatag ctgtttaatg tgttgatatt gattccccac gctctggcag
     5641 ctgcacttct tgaagggtag cgctttgtaa gcattccaat acgttcaata acaggttctt
     5701 ttccctgcga cataaagata cctcttggtg cttttctaag gaaaggtttc tttctattta
     5761 tccaattgaa aaataaggat aaaagcaaaa attagccaaa ataccaaaaa taccttgatt
     5821 tggtattttt ggtgatgcat tatttacccg tacagatatc cgcacgaata tccgcgcggg
     5881 taacttttta gaatagagga agtatgagta gaagagaagt ttcagatcac gattggccgc
     5941 ctgaaatcat caaggcgcgc ttacacatgg ctggcttatc actgcgctct ttatctttaa
     6001 aggctggtta cagcagggat tcgctgaaaa gcgtattacg tactccttgc cgaccgtatc
     6061 agcaaataat tgctgatgct cttggggtat cacctgaaga aatctggccc agtcgatacc
     6121 aggttaagag ctatatgaga aaggcgtcat gatatgtatg tcattgcaaa agaactgatt
     6181 ggcgcgcccg gaatgcctgc tacaacaaaa ggtattcgcc aggcattaca acgttacgta
     6241 caagggaaaa gctgttgttc ccgtcgtcgc tcaggctcta aagcaactga atacagcatc
     6301 gactgtttac ctgaagtgac gcagcaggca ttacgtgaac gctacgccct gcaactgatg
     6361 acgcaaaaag ccgatgaatc accggctccg gtggtgacaa aggccagacg ctcacctgcc
     6421 gtggttgatg cggtggaggc atatcgcgga tcaccccaac tgatggtcga acgcctcaat
     6481 gccctgactg aaaaccaacg taaagtggca gaggcacgaa tagcgatcgt cagcgaggtg
     6541 ctgaaaatcg cgcaacaacc cggctttagt tgcgctaagg ctatccggtt cctcgttaac
     6601 agcctggcac gatcacaact ggacgagcgc attgtggcaa tggttgagac ggcgaacgcc
     6661 aaaaagggaa acagccgcgc gttgagtgaa atcacgctga aacgctggat tgcggccttt
     6721 aacaaggcac agaacgccgc tgaacgcctg cttttactgg caccgggtaa acgccaggaa
     6781 ataaaagccg aagatattaa ctggctgccc gaatttctgg cgcagtatcg ccagtcaaac
     6841 ggccgaccaa tgaccgaggc ttacgaggat tttgttgctg aatggcagca ccggcacgct
     6901 gatgagcctt atatgctcga tatcatgccc tcttatgaca ccattcgccg cgcaatgaag
     6961 aaactgccgg aagtggtgaa acaaaaaggc cgggtaactg gcagtgaata ccgtcagctt
     7021 gagggattca cgcgccgcga ctggtccaga atgccggtga attatgtctg gattggtgac
     7081 ggtcacggca tgaagctgaa atgcgcgcac ccggttcacg ggcggccatt cgcaccggaa
     7141 gtgacctttg ttatcgacgg tggcacgcgc tttgtggtgg gctggagcct tgacctggct
     7201 gaaaatgttt tcgccgtagc cggtgccata cagcacggta ttcgccatca tggcaaaccg
     7261 tttctgtatt actcggataa tggctccggg gaaaccgccg acgtcctgga taaggagatt
     7321 gtggggatac tgccacgact ggggattaat cacccgacag ggattgccgg taatccgcag
     7381 ggacggggca ttatcgaacg gcttaaccgc acattaccga tgcgcatagc ccgtaaatac
     7441 cgcacctatt tcgggaaagg agcagatcgc gagacgttac gtaaaaccaa ccgcgattta
     7501 cgctcggcat tcagtgccct gcaacagggc aaacggctga acgcccggca gcagtcagcg
     7561 atgcgtgatt taccgtcctg gtctgaactg attgatgcca ttcgtgacgg tgttgagtgg
     7621 tacaacaacc ggccgcacga tgaattaccc atgaagccga acggtaaaca ttacagcccg
     7681 gcggagttca gaaaaaaacg cctggcggaa gaggacacgg aaattgaatg gctgtccgat
     7741 gttgaactgc gggatatgtt ccggccgatg gtggagcgcc ctgtaagacg ctgtgaaata
     7801 cgctggctga ataatattta ctacgcgccc gagctgcgtg acgagcatgg ccgtaaggta
     7861 cttatcagct atgacattca tgatgccgaa cgaattaccg tgcgccgccc ggatggcagc
     7921 gtgatttgcg aggcggtatg ggacggcaat aaacgcgaag ccttccctgt cagcgcggaa
     7981 tactacaaac agcagcagcg ccttaaaggc atgcgtaaac gcgcagagga aaaaatccgt
     8041 gatgccgagg atgaggttgt caacgtgctg gagcacaagc cgcaggagcc ctggctggaa
     8101 aacatatacc gccctgtggg taatacggtg accgttcagc aaccggtcgc tgacgatgaa
     8161 cctgatgaag aatacgagcg taatttccag cggggattgc aactgctcga agcgaaatta
     8221 aaagaaagtg atccgctggc ctgaaataaa aaaataaccc gaggggtgac tcaggttatt
     8281 tgaggtttca gtctcggaga atgctgtctc aagcgaggtg aataatatga ccgatattaa
     8341 cgatgtaatc aagaccattg atgaacttat tgatggcggc gtactgacgc agtatgccat
     8401 cgccagagag gcgggaattt ccgacggcac attatcggct ttccgtaagg gaaaatataa
     8461 aggcgataac gctgctgtgg ctgcttccct gcgctcctgg tatgagaact ggaataaaca
     8521 aagcgcactg ccggaaccgc cgcagtttgt ggaaactcag acagtccagg agctgcgcgc
     8581 actgtttcag gcggttcgcc tgatgggctg tattaacgtt attgtgggcg taccgggtgt
     8641 gggtaaaacg gccactgccc gtaattactg ccaggagcaa ccaaacacct ggatgatcac
     8701 cctgtcaccc gcgcactcca gcgtcacgga gtgtctgctg gagctggccg atgcgctggg
     8761 gattgattac acccgcgcga acaaaggggc attatcccgc gccatccgcc gtcgcctgat
     8821 gggaacgcgt ggactggtga ttgtggatga ggcggatcat cttggtattg acggtctgga
     8881 gcaactccgg gcaattcagg acgccacggg gatcgggatg gtgcttattg gtaacccgcg
     8941 cggattgttt aaaggtggac gccgcgcctt tgatgattta tcgcgcctgt tcagccgtct
     9001 tgcccgtaca aaacaacttc gcaaggccaa aaaggcggat gtgctggcca ttgccatggc
     9061 gtgggggatc agtggtgagg ccgagctggc cgtcatgcag gctatcgctg aaaagccggg
     9121 agcgttacgc gttctgacac atacgcttaa ccaggcgtgg ctcaccgcca gcggtgaagg
     9181 cgcggcgctg acagaaaaac atattaatgc ggcctttaaa gaggtttata ccaaccctga
     9241 attactctca caggtgtgat tatggcggta tttaatattc ctgatattta cggacgcttt
     9301 tacctggtta atttcgataa cgtgaaggtg atttcactgg ccgaaaataa agaatgtggc
     9361 gatttacttt ttgaatttaa tgaccgcaca cgaatggtga tatctgccgg acttgatcgc
     9421 gaaggtgcga cagaagttta cagcggaata tgccgttctg ttggtgcgaa acaagtcagc
     9481 taaatgaggt gttatatgaa tatgcaatcc tgcggtaaca aaatgaattt attcgactcc
     9541 ctgaacagcg cgcgccgtct gaccgaactt gccggtgcgg tactggaacg cagtaagcgc
     9601 tatccgcaac gttttgcact gaaaaccacg ccgccggtag gcaacgtgca gggaaccggt
     9661 gaaattgaaa tcaccataca gaccaacggc ctgcgccgcc gtgtgaaggc cacccgcatc
     9721 agcggctgca cggtttactg ggaggtgtga ggtgaaaaaa aatctcattg catgggcgtg
     9781 gtcgagtggt cttattgagt ttggttacgt cctgccggaa ggtgcattgc cgatagttgc
     9841 cggaaagcct gccacggtac ggcatgtgat tgaggttatg gcgcgtcatg gacgtgatga
     9901 acaggagcag ttactggttc cggggatacc ggaagcggtg acggaggaag aagcctttaa
     9961 tgccatgatt cggttctgcc gtgaggttag acgccgggtc agttatccaa acagaacgag
    10021 gaccagagga tgagtaaagt cgtacgcatt attttcgaat acaaggaaga tgttatctgc
    10081 aaaaactctg atggcagcac gcacagaggg gtaagtctgg atatacgttc aaccggaata
    10141 aagaagaaag ataatggacc cgccatgatt tttggggtgg ttatgctggc agaaagcaga
    10201 aacttcgcag agtttgtggc aatgaaagcc agtgcattca tgaaagacag aggcatggct
    10261 tccggggtta ttaacggtaa tgaatttaat caacaggggt aattccatga gcaaagtacg
    10321 cgttattttt gaatttaatc atgtttcgca tgacgaaaag ttggcaggca atgactgtgt
    10381 tgaagtgcat gaaaagattg gagtggatgt gaaaacagaa cgtgatacgg ataacaggcc
    10441 gacgtcactc tgtgacgttt atgcaagtat tctccagtat cacagtcctg caattattca
    10501 gtttctctca gcggaatttc aggcatctgc acagtctttt ggagcggatg ccatcattaa
    10561 acgccaccgc gtgcataaag catcaggcac actgcaataa ggaaaaacaa aatggctaaa
    10621 cgcgttacaa aattaaaggc cgcagccgag gcggcaccgc agacccggga agaagtcagc
    10681 cgtgatatcc gcaccctggg agatattcag cgagaggcgc tgcgcctgga aacggcgatg
    10741 aatgacgaag tggcagaaat caccgcccgt tatacgccgc agattgaaaa tcttaaaaag
    10801 caaatcaaag tgctttttaa agggattcag gactggtgca aaaccaaccg tgatgagctg
    10861 acgaacggcg gcaaaaccaa aacagccaat ctgaccaccg gaacggtgtc atggcggctg
    10921 ggaacgccat catgcagcgt cagccgtgat gtggaaggtg tgattgaaat gctgcgccgt
    10981 atggggcttg agcgtttcat ccgcacgaaa gaggaagtga acaaggaagc cgtcctggcg
    11041 gagccggatg ctgtgaaagg cattgccggt atcaaggtga ataaaggcgc tgaaagtttt
    11101 tatgtcgagc cttttgaaca ggacgccgga ctgaataaat aacaccgcat taatcaatta
    11161 aatatcactt cattttaatt atggcgctcg cgtcagggga ctgcttgcgc ctgaaaacag
    11221 aaaataaggg ttaagaaata tgaaatacgt ttatatcgtc attaataatg tggtggggaa
    11281 ataaatcatg attgatgcaa aagtgcttga aggggttaaa aactggctgt gtatttatgg
    11341 ccgcctgacc tgcggtgtac tggctgaaaa aatgaatatg ccgccatcct ccatggttta
    11401 ttttttgcgt gatgcggttg atgcaggcgt gctgacggaa tgtaacggtt tttatgatat
    11461 tccgcgtccc cgcccggtgc agccggttcg tcgcaaatgc agccaggaat ctgcggctga
    11521 tgatgttcag tggtgcggct tcagaaaatc cctgccatgg attgaggggc atgatattcc
    11581 gtcgatggcg tgggaatttg ctcagggcgt tctgacctgt gaaaccgttt atgtggtggc
    11641 tgaagttgat gagcaggcca tgaaagaagg cgtgccccag tttgtgatgg cgtatatcga
    11701 catacgcctg ggcgtcatta tctgcggttt aagcggctgg aatatcactg aacatgttct
    11761 gcgctacctg attgttgacc ggacagcagc gcctgccggg atatctgcgg aggtggcgta
    11821 atgttcttta aaacatcaaa cccttccgcg ctggctgcgt ggcaaaaata ccagcaggac
    11881 tgccagaaag ttaaggatga ggcaaaacgc cttgaggccg tgctgaatgt tgcgtgccgg
    11941 tcggtatttg tatccggtat cagtggattt tactttaagg gactgcgttt tacggaggac
    12001 aaatatcctt ttcatcgcga cttatggcga aaaccgactg cgtcgaatgg ctggagctgc
    12061 acaccgcgca catcacgtat ccctaaaacc ctgcgtgttg cctctgatga actgaacagt
    12121 ctgtggcgtg aatattcgcc cgtcacgtat gccagaaccg atgcgctgtt gttctggttg
    12181 ggtattgatt tctcggcaat cctgtttggc cctgtgaagt ggttctgcgt tgacgatgtg
    12241 atttaccttc agtgcggcgt aaaacccgca aaacagaaaa tgaccgaaat actgtctgat
    12301 gagttttatg ctgctgaaaa gcgagtcagg gggtgatgca tgatgaaatt acaacccatg
    12361 ggactaaaag gtcgcgcccc cgctcatgtc cgtccgtgga cacctgaaga agatgcgctg
    12421 ctgattgcgc tttattcatc caccccggtt aaggatattg ctgccagaat aaaaagaact
    12481 gtctgggctg tatataaccg gactggtgta ttgcgcagtt catacccgga gttactgaaa
    12541 tataaacacc caagatttac acctgatgaa gataagttta ttcgaaaaaa tgccagaaca
    12601 atgacctgcc agcaaatggg agaatatctc ggacgtaata aagattctgt cagatgtcgg
    12661 gcaggaatga ttggtgctgg attaacaaag tgcggagagt tacgccccgg aacgcacata
    12721 tctgatgatg atgtacgtct tatacgtgcg ctgcgtgatt ccgattaccc acgccgtctg
    12781 tcattccggg aaattggcga aaagtttgga atatctgaac attctgctca cgcagtttat
    12841 taccgtcgcc ggactgccga ggacgctgta ttacgggagt taacgccatg ataacgacct
    12901 tatttgttga atcagatgaa cctcttgtgt gtgccgccgg aatgccgctc tgtggcggaa
    12961 cgctgaccgg tgtttatttc ggggatttac gcggttatcc ctggcattca ctgaatgatg
    13021 ctttcccacc tgatatggag gctgccgtgc tgattgttca gtatggtcac cgtcaggagc
    13081 tgcgcatcgg ccatatgggg tatgaaggct tttttgttga tgaagaaacc ggagcctgcc
    13141 ttgaagatga agacggacag gtgacgcact ggtgtcatat ttcggcttta ccggaattac
    13201 gggaggtgaa tgcgtgagaa gttatattga taatgaaaag ctggagacaa taagcgactg
    13261 tcttagcttg ctggcaaaaa tcaaagaaac tattgaagag attaaatttc agcttgaata
    13321 cgcaccatgt ggcgatgatg catggcgtaa tgctgccaga aaagcactgg ctgtctttca
    13381 gaaacaacgc cgggctgttg aataccgcct tgctgtattg cgccaggagg aaaaggaaag
    13441 aaatataaga cgtcatgaac gcgttaatga tttccttgtt cgtgaattaa aagagcgcgt
    13501 gccggagtcg gtgttttttg aatgtgaggc tatagcccgt agcaaagcac tggaaacaga
    13561 ttaagcaggc aggtgaataa tgagtattga attaagatcg tcttatgaat atcgcaaaat
    13621 ccttatcgcc ggaggcatga aaccggaaga tgcagagaaa ttcgtttctt ttatggataa
    13681 agaatgtgac aagcgggata tgccagaaat tattatggat gacatgattc tggattcagc
    13741 cgtggcgtta agtccgttat ggattgtgca tactcttgcg gaaatagcta aaagaagtga
    13801 caagcaggcg gctgtcgccg ccctgcgatt actggctgaa ttaagttgtt cagtcaatcc
    13861 caatacgttc tttgacgaac ctggttaaca ttgatttggt aacttcttta attagatcaa
    13921 atggtgcgtc tgtaaactcc tttttgattc tttcaaggat tggttttttt ctcagggcgt
    13981 tagctaaatc atgcccctgc tgggtaagca taatgggcgt gtttctgaaa ccaacgccca
    14041 gagaatggaa aaagaaacca atatatttgg gtgtttctga ctgcatatca ccattaacaa
    14101 ttgagccatt atcagcgaga agcataagat gaaaaacttt tttatccttt tcctctcctt
    14161 ctgcctcagc catgcccaga tcctgtagtg taaggaaagg tgtttcagca tcctgaaaag
    14221 ttgtgagaat acttaatact tcatcgtaat ctattttcat atcgatacct ttagttttat
    14281 ccgggcggcg ttgccatgcc gcctgttgtt acggagacac gataatgaat cgcgcttccc
    14341 tgattacctt aatacatgtc gcaaaacgcg atctacagct tgaccatgag acctacacat
    14401 ccgtactact ggcagccacc ggcaaaccca gttgccgtga tatgtcaccc ggtgagttat
    14461 cccgcgtgct ggatgttttc aaaaaacgcg gttttaaagt gcgtcagaag ccggttaacc
    14521 gggccttaaa accgggtacg gtgaccgcca aaattcgcgc catctggaaa gtgatgcacc
    14581 ggcagggatt tatctctgat ggcggggaaa cggcccttaa ccgctgggtg aaatcgcaga
    14641 cggccgcgca gaacgacggc gaaggtgtgg ctaactggca gtggctggag caacaacccg
    14701 cgctggcctc tgatgtgctg gagcgtctca aacgctggca ccgccgcaaa atgctggcag
    14761 ccctggatat gtcggaacgt gcagtgatga gttatgagcg tgtttgcagg ctgtatgaaa
    14821 aatcattccc ccgttaaccc caaatcccgc cacagcgcgg gatttttatt ttaaacttgc
    14881 cctgtgtaat gatgaccgga ggcgggtatg gctgaaacac agatgagcat gtttggtgac
    14941 agcgaacagc tgcacgcgct gattgaccgt ctggatgata ttcctgatga tgagctgaaa
    15001 aagaactggc ccggcacgct gcgggacctg gttgatgtga tcagtgctga actgcaccgt
    15061 caggggattg aacctgctca ggcgggaatg cttgcccgta aggttgcagc cgcacaggcc
    15121 gggtacatgg gcgggcgtgg ttattatctc ccggtcggtg aatccctttt caccgaactg
    15181 cgcaataatg aaattttttc ccgctggagc cagggcgaaa aaattgaaac cctgcgccgc
    15241 cactaccaga tgtcagagac gcagatttac tccgttatcc gcaaccagcg tcgcctgcat
    15301 caggagcgca cgcagccccg gcttttctga tattccgcaa atcctgcccc cttttcctca
    15361 cggttacgct gactcagaga acaccatgag acagcgtaac aatgccaaaa ctccccgcac
    15421 ccctgcgtaa aaagctgatt gcccttgttc tggccggagc cgggacgttc accattgcca
    15481 cgcactacac cggctactgg gaagggaaag aaaacaccac gtacattgat cccaccggca
    15541 cccccaccat ctgttacggc cataccggcc cggatgtgaa accggggatg acaaaaactg
    15601 atgaagagtg tctggagctg ctggaaaagg acatgaaatg ggcctttgcg gctatcgatc
    15661 gtcatgttca ggtgccgctt acccgtggtc agacggtggc gctggcctcc tggattttct
    15721 gggccggtga aacgaacttt cgcaactcca cgctcctgcg ccttatcaat gccgggcaga
    15781 tgcccgcgtc ctgtaagcag tatatccgct ggatttattc aaagggggta aaactccccg
    15841 gtcttgaggc ccgccgttcg gcggatgaat ggttatgtcg ttacgatttg ccgaaagtct
    15901 gaaccgcttc tggcgaccgc tcatgattgc gctgctgtgt gccgttgtgc tgctgcgggg
    15961 tgtgctgtgt ctgcggtgaa caatccctta ccggccctga ttgccggagg ctgtatggcg
    16021 gcgctggggg tgatgagcgt gctggctgcc gtggtatggg gaatgcacca gaagacgcag
    16081 cgccttgagg acaataatca tgtgcttgtg cgtgaacggg acgaagcccg tctggtgctg
    16141 gcaaaccagc aacgcaccct gcaactcatt tcacaaatca gcaaggcggc cacgaatgaa
    16201 aaacagcaga acatgcagca cagcgaggaa cagcagagca ttgtccgccg gtcactggca
    16261 acggtgcctg ctgccgctgt gcctgtgcct gatgatgtgg ctgaccgggt gcggcgggcc
    16321 gtctgtgaaa tacgtgccgg tgaagccggt gccgatcccc gctgaatggc tggctgactg
    16381 cctggtccct cccgcgccgc agccgttcac gtttggtgca tcggtcactt acaacctgca
    16441 actgctggcg gtgattaaaa actgcaacgt ggacaaggcc agcatccgcc gtctggagac
    16501 acagcgacag catgaattta ctgatatggc cggaacgact gctgttccgg caggaaagac
    16561 gaagtaagga aaaaggtatg gatgattcag attgtgctca ggcagtcatg gagcgtgcaa
    16621 cagaacgcgc cctttgtgac cggctgacac gtaagcgccg ggtggcggag acaccgggca
    16681 ggcgtgtatg cgcggactgc gggggtgaca ttccggctgc ccgtctggtt gccgtgccgg
    16741 atgccatccg ttgtgtgaac tgccagaaca tcatggaggc cggtcatgtg ggtcagcatc
    16801 gttaaagatt atgtcgtgcc gatcctgtcg gcaacggcca cagccggggg gatcttcatg
    16861 gcgctgatgc gcaaaacctt tgtcccccgc gaggcctttg aaaaactctc cgaccgggtt
    16921 gagcgggtgg agagccggat ttccggcctg cccacgcagc aggaggtgaa cagactcaat
    16981 atcgaaatca ccaccctgcg cggtgagctt aaggccacca atgccaccct gaaatccgtc
    17041 tcccatcaga acgaactgct gctggagcag gctgtaagga aaaaacacaa tgagtgattt
    17101 cattactgaa gatcagcgtc ttgtcattct gcggtcgctg gcggattaca acggcgaact
    17161 gggcgaatcc gtgcttcagg actgcctgga tgattacggt caccgcgtgt cccgcgacac
    17221 cgttcatacc cacattgcct ggcttgccga gcagggactg gtgcgtaagc gcacgcttgt
    17281 taacggttat ttcatcgctg aactgaccgg ccggggtcag gatgtggcgg aaggccgcgc
    17341 caccgttccg ggtgtgaaga aaccacgcgc aagggggtaa cgatggataa gccagcgcgt
    17401 ggccgtgtac gcaaggtgga tttgctcccc gacagcatcc gtaagccgct gctggaaatg
    17461 ctgcgtgaaa aacgtctgac gcaggtccag atccgcgagg aaatcaaccg tctgatccgt
    17521 gaagcaggtc tgccggaaga gcagcagctc tcaccggcgg ccatcagccg ggaagcctcc
    17581 cgtaatgagc tgattgcccg taatctgcgt gatttacgcg agcagacaaa agccatgatg
    17641 gccgaactgg gggataagcc gaccggtgaa accacggcac tgattctgga gatgtcccgt
    17701 gccctgatgt accgccgtct gcgtgccgcc accgaatccc tgaacagcga cagcgatgtg
    17761 gatatgcgcc ttattaagga catcctcctg tcggcacagc gtgcagaaag tgccgccgag
    17821 cgcagcatta agcgtgaaaa agaaatccgg gccgcatttg ctgaagagat ggcgaatgcg
    17881 gtcaccgatg agttgcgcgg cgtggatggc atgagcgagc agatcgaatt ccgtatcagg
    17941 cagatgctgc tgggtaaggc gtaggaggtg aatcatggca acagataacc gctcactgat
    18001 gagcagtgcc catcagaaag cattcagtct tgcaacacag ttacctcctg aaaccataaa
    18061 ggtatgtcgt gagttgactg aactcagcat tgccggggaa atatccctgg aacaactgca
    18121 ttcgctgctg gcagaggctg ccactgatga gttgagcggc gtggacgtga tgactgaaga
    18181 gcttgagtcc cgaatcaaaa gcattctgac aggtaaagca tgatgaacaa agccagagaa
    18241 acgtcgcctt cactgatgca aatgacgcct ccccgcaaaa ttgatctgtc aggtgaaaaa
    18301 gccctgctgg gcgtggatgt gccggacagc cttgatttgc ccggtgatat gccggtgttt
    18361 ctggattatc aggcccgctg gtttgcagat gaaagtcagg tctgcatcgc ggagaaatcc
    18421 cgccgcaccg gtcttacctg ggcggaagcc gggcgtaacg tcattactgc tgcaaaaccg
    18481 aagcgtcgtg gtggccgcaa tgtgttttat gtgggatcaa agcaggagat ggcgctggaa
    18541 tatatttctg cctgcgcgct gttctcccgt gcctttaacc agctggcaga tgctgatgtg
    18601 tatgagcaga cattctggga tcgggataaa aaagaagaaa ttttgaccta catgatccgc
    18661 tttccgaaca gtggattcaa aattcaggca ttgtcttccc gtccgtcaaa cctgcgcggc
    18721 cttcaggggg atgtggtgat tgatgaagcc gcgttccacg agtcactgga tgagcttctc
    18781 aaggcggcaa tggcgctcac tatgtggggc gcgcgcgtgc gtattatctc cacgcataac
    18841 ggcgtcgata atctgtttaa tcagtacatt caggaagccc gcgaggggcg caaggattac
    18901 agcgtccacc gcataaccct ggatgatgcc attgcggacg ggctgtaccg tcgtatctgt
    18961 tacgtcaccg atcaggaatg gtcaccggaa agcgagcaga aatggcgtga tgatctctac
    19021 aaaaacgccc cgacccgtga ggatgccgac gaggaatacg gctgtatccc gaaaaaatcc
    19081 ggcggtgcct atatccccca cgcgcttatt gaaatggcga tgatccgcga catcccgatt
    19141 ctgacgtttg aagccccgga caacttcatc agccgtgccg catggctgcg tgaatcggaa
    19201 gttttaacct ggtgtgaaga acatttaaaa ccgcttttag agaaattaaa tccccgttcc
    19261 cgcttcagct tcggggaaga ctttgcccgc accggtgacc tgtcctgctt tgtgctgctg
    19321 gaaatcaccg aatccctggc aaagcgcgag gtgtttcgcg tggagctgcg caacctgccg
    19381 tatgcccagc aggaacaggt gatgatgtac atcctgaccc gcgttccggc gctggtcggt
    19441 gcggcgttcg acgccaccgg taacggcggc tatctggcag aggccgcact gctggccttt
    19501 ggcccggaca tcatcgactg cgtgatgttg tcgccgaagt ggtacggcga gtggatgcca
    19561 aaactgaaag ccgagtttga agatcagaat atcctcgtcg cccgccatca gaccacgctt
    19621 gatgatttgc gccatgtgaa ggtagtgaat ggtatcccgc aaatcgacaa ggggcgcacg
    19681 aaggaccaga acgcaacggc tgcgaacgcc cgccgccatg gtgattttgc cgtggcgctc
    19741 tgtatggcaa accgggcgtc atacatggag ggcttcatcc tggatgaatc agcctgtcag
    19801 gcgctacagg agcggtcgcg ggcaatggag ggcggttatc gtgatgacga tgaggcatat
    19861 catgaatttg atcggggggg ctggtagtgg gacgcatcat tgatactgac ggaaaacctt
    19921 tctcctttga cccggagatg cagagtgccg cgctggatat tccgcagatt gccagccgtt
    19981 atattgaaca tccggcttcg ggtatcaccc cgaaccgggc ggcgcagtgc cttcgcgggg
    20041 ctgaacgtgg cgatctgatt gcccagtccg atctggccgc tgatattgaa gaaaaggaca
    20101 cccacctttt tgcggagctg ggcaagcgac gtcttgccat tcagggggtg ccatggagta
    20161 ttgagccgcc accgaacgcc agcgcgaatg agaaaaagga cgcggaaatg ctcgacgaat
    20221 atctgcattc cgcagactgg tttgatgcca tgctgtttga cgccacggat gccatcctga
    20281 agggctattc ctgcatggaa attgagcacg ggatgctcgg taaaatgcac atcatccgcg
    20341 ccatccgctg gcgtgacagc gggcatttct gccttaaccc ggatgatttg agcgaactgc
    20401 ggctgcgtga cggcagccat tccggggtgg cgtttcagcc ctttggctgg atagtgcatc
    20461 agtcgcgttc acgcaccggt tacggtggtg caaccgggct tgtccggacg cttatctggc
    20521 cgttcatttt caaaaactat tccgtgcgcg atctggcgga atttctggag gtgtacggcc
    20581 tgccgatgaa ggtcgggaaa tacccgtccg gggcaacacc ggagcagaaa agtgccctga
    20641 tgcgggcggt gatggatatc gggcgacgta caggcgggat catcccggcc gggatgtcgc
    20701 tggagtttca ggcggcagcg aacggtcagg ccgatccgtt tgaaaccatg atttcgtggg
    20761 gggagcgttc catctccaaa gcaatactcg gcggcacgct gaccacggaa gccggagaca
    20821 aaggcgcgcg ctcactgggt gaggtacata acgaggtgcg ccgggaaatc agggattctg
    20881 atttacgaca gctggctgca accctgaacc gcgatctggt gtatccgctg tacgccctga
    20941 acaccacgca cactattgat atccgccgtc tgccacgtat ctgcttccag acaaaagaac
    21001 caggtgatat caccaaaatc accagtgcgg tgatgcagct cagtacgggg atggatattt
    21061 ctgatccctg gatacgtgag cagaccggta ttccgcagcc cacgcccggt gaagccatat
    21121 tccgtgtccg tcagagtggc aatgaaccgg ctcagaccga cagggaaaca ccaccggaaa
    21181 aacaggaaaa aacagagcag acggcgctgt cagccagact gccggaagcg aaaagcacgc
    21241 cgcgtgatga actggatgat atgggggacg cggtgcctgc ccgccgctta caggaggcca
    21301 tcgacccggt actggagccg gtcattgatg ccatcagaac gcgggggctg gcggatgcgc
    21361 tggcagacct gcctgccctt tatcgtgaaa tggatgattc ccgcctgatg acgctgctca
    21421 gtgatgccat gtttgctgcg gaaatgaagg ggatgctgga tgacacaggg gattgattta
    21481 ggttatgccg ccacgcttcc ctcaaaagag gcggtagcat acttccgtgt caaaggggcg
    21541 catatcagct ggaactggtt cgaaacagcc gcggatgttc atgcccgctc attcacggca
    21601 gcaaaagcgg cacgcctgga tgtggtgacc acgttacagg ccgaagtgca gcgggccatt
    21661 gatgagggaa tttcacagaa agcatttatc cgcacgctga cgccacgcct gcaaaagctg
    21721 ggatggtggg gaaagcagat tgtggtggac agcgacggta acgcggaaga agtgcaactg
    21781 ggcagtcccc gtcgtctggc gctgatttac aacgtgaaca cccgggtggc ttacaatgcc
    21841 gggcgttaca cgcagatgat gaacaacacg gacacgcatc cgttctggca gtatgtggcg
    21901 gtcatggaca gccgtacccg cccgtcgcat tccgccctta acggtctggt attccgctat
    21961 gacgatccgt tctggaaaac acactaccca cctaatggct ggaactgccg ctgccgtgtg
    22021 cggccattgt ctcaggctcg tctggatgca atggggttat ccgtttcatc cggtgaggat
    22081 catctctcca cccgcaatgt tgaggctggc gtggataaac agaccggaga agtcagagaa
    22141 atgccggtga ccacatattc agatggcacc agaaccatga caccggatgt gggctggtca
    22201 tataaccccg gctcggcagc gttcggcaca gaccaggcgc tgatccgcaa actgatcgag
    22261 gtgaaaagcc cggcgttacg ggaaatggtg gttcaggaga tgaacaacag cccggagcgg
    22321 cagctggcgt tccgcatctg ggcaaaaaac atcatgcaga cccggcgagg tggtaacgat
    22381 atccgcacgc tgggctttat gactgaaagc attgcgcagg cagtggaaag ccggacggga
    22441 acgccaccgg cccgcctgct ggcgatgagc ggtaaaaatg tgctccatgc agacagtgtg
    22501 aaacatcaga atgacggcat cgccctgatg ccagaggact tcgggcgctt accggcaatg
    22561 ctggcaaagc caaaagcggt gctgtgggac aaacggcaca aaaatctgat gtatatcgtg
    22621 gaaaataagg atggcagtgt gcagattgcc gttaatgcac cgtacagtct gaaacgtcag
    22681 cccgacaaac tggatgtgat tgtgaatgct taccgggtga tcaatatgga taagctgaaa
    22741 tcggatatcc ggggcggaat gcttgaggtg ctggaaggac aaattgatta acggtcagtg
    22801 gcgggggtcg aacccgcata catgatccac ccggaggcag gcaccgactt taccagttaa
    22861 gcgtacactg accgcctgtt tattttatat tcatccttta tgcggaggca atatgtcatc
    22921 cattgatgcc gctgtcgttg ttgacgttac gcgtctccag cgggtgtttg cccggcttca
    22981 gtttgtgggc ggcggaaaag acctggcccg cagtgtggcg gcgagtcttc tgtcatcgtc
    23041 agaaatggcg tttgaacagg aaaaagagcc ggacggcgaa cgctggcatg actggtcaga
    23101 tccttaccgc aagtggcgta cccgtaaggg atacacgccc ggcaaaatcc tgacactgaa
    23161 cggcgatctg gcccgccgcc tgaccacgga ttatggcgat acctgggcgc tgattggatc
    23221 aaatgaacct tatgcggcca ttcatcagtg gggtggcctg cctggtatgc caccgggacc
    23281 ggcggccatt ggcgcacgtc cgtatatggg ctttgatcag gtggcagaac aggagatcat
    23341 ggacgaaatc agaaaacgct ttaaaaaggc cacagaaacg ccgtaatggt ttaaggtata
    23401 caaacgcatt accttaccct cttcaggcgc gtgtcgtgat tttctaacct gtatttaacg
    23461 ggctttaaaa tctgcgtgaa gcgcctttct catttttccc ctgtcagcac tttctgaagt
    23521 cccgcaaaac cggccccgaa aaacaccggc ttatgctgcc ggaatgaaga cgaaaaacac
    23581 gcccaaactt gcttatgcca ttctgaatgc catcagcctg tccgcagacg gggacggtga
    23641 ctggtgtcag atcatgccag ccggtcgggt aaaggcccgt gacggtcgcc cggaaaaacc
    23701 ggcggaaggc tggctgatta accatgcggc cgttgagcgc atggtatccc gggttgtggc
    23761 gctcaatcag ccggtaaaaa ttgactacaa ccaccagacc ctgattaagg ggcatccggc
    23821 accggctgcc ggttttgtca tggcctcacc ggaaaacttc cgcttcagtg aagagcgggg
    23881 ttttgaagtg cgtccggact ggaaccctcc ggccattgaa cacctgcgca ataacgaatt
    23941 tccctggttt tccccggtga ttggttatga cgaaatcacc ggtgaacctg ttgagcttcg
    24001 gatgctggct attaccggtg accccggtct gactggcatg aatcctgtcg ccgcactgtc
    24061 ggcggatgac ctttataacg ccttaaaccc tcctttaaag gacacctcca tgaatgagca
    24121 attacgccag ttgctgacgg cgctcggtct gaccgtggcc gacggtgacg aatttacacc
    24181 ggaactgggc acggcggcgc tgtctgccct taccgggatc aaaacccgtg cggatgcgca
    24241 cgacaccctg aaaacgcagg ttgccagcct gtcggcagag ctggaaaccg caaaaggcac
    24301 accaactggc ggcactattg atctgacgaa atacgtgccc gttgagacgt ataacgccct
    24361 gcgtaccgaa tatgtcgcgt tgtcggcaca acacggcagc accacgctgg agcagttgct
    24421 ggataaggcc gagtccgaag gacgcatttt caaaagcgaa cgcggataca tggaaggact
    24481 cggacaacag attggtgttg cggcactttc tgcacagctt gacgcccgcc agccggttgc
    24541 ggctctgacc actcttcaga ccgacaccgt aaccgtgccg gataaaaaga ccgccaccgc
    24601 tgcgctgtcg gctgaagata tcgccgccgc ccatctgctg ggtaaaaccg aagccgagtt
    24661 cctgaaaatg aaagaggaaa tgcaataatg cctaccccga tcacaccggc gatgatcacc
    24721 gccctgatga cgggctaccg ttctgatttt caggccggga tgtccatggc tccgtctcag
    24781 taccagaaaa ttgcgatgac cgtaccgtcc acgtcgaaat ccaacaccta cggctggctg
    24841 gggcagttcc cgcagttccg tgagtggatc ggctcccgcg tcatcgagaa gatgaaagcc
    24901 tatggctacg ccatcgtgaa caaaaccttt gaaggcaccg ttgccattag ccgcgatgac
    24961 tttgaagacg acaatcttgg catctactcc ccgttgttcc aggagatggg gcgcgctgcg
    25021 gcggcacaac cggatgaact ggtctttgct gcactgcgtg atggtctgaa tgcggcctgc
    25081 tatgacggcc agaacttctt tgataccgaa caccctgttt accccaaagt ggatggctcc
    25141 ggtgatgcac agatggtcag caatatgttt gtggcaaaaa ccggctctgt cggtgcacag
    25201 gcggattaca gcggcccggc ctggtatctg cttgactgct cccgcgcggt aaaaccgctg
    25261 atttatcagg atcgccgcaa ggccgaactg gttgcccaga caaaagtcga tgaaggccgt
    25321 gcgtttactg ataacgagtt tgtgttcggt gcttccgccc gtcgcaatgt gggctacggc
    25381 ttctggcaga tggcctacat gatgcagtca ccgctgacgc tggatgcgct gtggcacggc
    25441 tggtcagcca tgcgtgactt taaggctgac ggtggccgca aactcggcat caaacccacc
    25501 catattgttg tccccacctc gctggaaaaa caggcggtgc agctgctgga gcgcgagctg
    25561 ttcgcggacg gtaacaccac cgtctctaac gagatgaagg gcaagctgga gctggttgtc
    25621 gcggattatc tgtaaacggt gagccgggct tacggcccgg cccctgcgga gggcaaatat
    25681 gaatgaacat cacactgtgg cagcaacaga tgattcaggc cttcaggtca gtggtgacag
    25741 tcctgtcacg gtactggcag aagtgcgttg cagtcgtgcg cagtttcgtc gtgcggggtt
    25801 cctgttcatg cggggacgcc agcaggttga cgtcaccccg gagcagcttg cccgactgga
    25861 agcggagcca tgcctcactg tacgaatact acagacgcct ggtgatgatg cgggagccgt
    25921 ggcgggtgtg gttcatgcag agaccggtgc agatttagcc gaagccggac aggatgctcc
    25981 ccggaaaaag accggcaata acgccagaca ggcgaaaaag gctcgcgcat gaattacgcc
    26041 accgaaaccg atatgcgggc acgttaccgc gaggatttgc tcaggccatt actggccgtg
    26101 ccccgttcgg atgagccgga cgcgcgcaag ctgaaccggg cactgacgga cgcgtctgcc
    26161 cttatcgaca gttacctgtc tgcccgttac acgctgccgc tggcggtggt tccggcggtc
    26221 cttgttcagc attgctgcac gattgccttt tattacctgt gcgatcagcg ggcatccgat
    26281 caggcgcgtg accgttaccg tgaggcgctg gcctggctga aagatgtcat gaacggtaac
    26341 gtgccggtcg gcgtggatac agacggtgcg gcccctgaat ccggggattt accgcaggtg
    26401 cagtctgatg cggcggtgtt cgggcgcaac cagaagggct ttatatgatt acggaaaccg
    26461 agcaagcgta catcgcccgt atccgtgagt atttcgggaa tgaactggtg tcagttgaca
    26521 cgcatcccgg cgactggagc gacagtgtgc tgcgcaccat gctgattagc gccccggcga
    26581 tctacgttgc ctggctgggt gccggtgaag gccgtacccg tgggcgtctg gtcagccact
    26641 gggtgttcta cgtcatcggc gacatgctca atggccgtga ggtcagccgt cccgggcttt
    26701 atcagattgt ggcccgcctg attaccgtgc tggacggctt cagaaccgaa aaaacctcac
    26761 cgctttactt tgaaaaggcg gtcaacggtt acaccgaaac gcaggccgac agtggcgcgg
    26821 tgatgtatgc gctttatttc tcctgcgagg aaatgatcgc accgctgact gatatcagct
    26881 cgctggatga tttcctgcgc cattacgaaa ccttcaccga accggaaggc acaccggaat
    26941 ttaaggcgca catcagcctg ccaggtgcaa ccgccgccgg tgaaaaagcc ggaccgtcag
    27001 aggaataaca gatgacaacc atttttatca aacccgcgcc gggatgcctg atccgcgatc
    27061 cggacaccat gaaaccgctg gcgcaggagg gagaggaaaa acccttcacc ccgttctggt
    27121 gccgccgtct taatgacggg gacgtcattg tggtggaaaa agcggctgaa gcagcaccgg
    27181 cagcagcgtc tgcaacgact gatgccgccg gaaaacccgc gggagcggca gcagaaaaac
    27241 ctgcgactga aaaacccgcg actgaaaaac ccgcagcatc agacaagggg acgtcctgat
    27301 gattaacttt gattatatcg gcgataacaa tcgcattccg ctggtacaga ttgaaatcaa
    27361 taattcaatg gccgtgaccg gcactccacc acagcgtcag gcggtactgt tgtttggtca
    27421 ggctgcaatg aaagacacca ccattcaggg gcgcggtcag cttgatgtac cagtacgtat
    27481 tacccgagct tcacaagcaa gagaactctg ggggcgaggt tccatgattg cactgatggt
    27541 agaggaattt atcgccatta accctgacac agagttatat gccattgcac agggggttgg
    27601 aacaggtcag tccagggcat gcgtgatgaa tatcattgga acagcattgg cagacggggt
    27661 gttaagtgtt tacatcggcg gtcgccgtta cctcctgccc gtcagtaagg ggaaaaaagg
    27721 taaagagctt gtggaagagc tggctaaggt cattaacgct gacactgatg cgccattcac
    27781 tgctatcgca gctgaagtca gtggtgataa tgctgaagga cttaaaggga gcatgggggt
    27841 taacgcccgc tttatcggtg aatgctcagc tcatgattta cgggtgaact attatgacgg
    27901 tgaaactaca ccggccggtc ttacgctgga gctcgtcgcg ccaacacaga aggcaacaaa
    27961 ccccgatatc acccgtagcg tctcagggat ggggaatcgt cagtacaact acatcgtcat
    28021 gccctataag gatgcggcaa atctcaaaat tctggcggat gaactgctga aacgctgggg
    28081 accggtaaaa atgtcggacg gtatggtctg gatggcgcat accggcacct tcggtacggc
    28141 aaccagtttt ggtgaatccc gtaatgactt cctgtttacc tgcacttcta ttcctaaagc
    28201 accagagccg gattatgtct gggcggcctc catttgtgcg gtctgtgcgc cctccctgtc
    28261 aacggatccg gcccgaccat tacagacact ggcgctgcct gcccgtatgg cacctgaagc
    28321 accggctcgt ctgacgcggg aagagcgaaa cagcctgctt tataccggca tgtccacggt
    28381 gaccgttgcg gccgctgatg tggtgcagat tgaacgtcag gtgacgatgt accgtcagaa
    28441 tgcatacggc gaaagcgatc ccagctatct ggatgtagaa accatctaca ccctgtcata
    28501 tctgcgttat tccctgcgca cctttatcac gcagcgtttc ccgcgccaca aactggccga
    28561 taacggtact cccgtccggc ccggtcagaa tatcgtgacg ccggagatta tgacgctcca
    28621 gctgattgca ctgggggaag aatgggtgga actcgggctg gtcgaaaatc tggacacctt
    28681 taaaaagaat ctgctcgtgg agcgcaacac aaaagaccgt aaccgtctgg atgtgatgtg
    28741 tacaccggat ctggttaacc agttccgctt catggccgcg cagatccgtt tcattttgtg
    28801 aggtaaggca tgagcggaaa acagcatcag ggcacggcca ccatccgtgt gaacggacag
    28861 gagtacgcca cccttgaggg agccacgttc tccccgtccg gctttgagcg tgaagtggtg
    28921 aaaggcgcga aagtctatgg ctatcgtgag aaaccccgtg aggcgacgct ggactgtaaa
    28981 tttccggctg gcggagaagg ttcaccggct gccgatgaaa tcaacacctg gactgcggtc
    29041 acgattgagt tcgtggccga taccggtgaa gtccatatga tgacgaaggc ctggagcagt
    29101 gaaccggcct cgcttgacgg tggcggtgat atctccgcga agttcgccag cgccaccagt
    29161 acccgtgtcc agtaatcagg aaaaatgcaa tgaccacacg taagaaaaaa acggcggttt
    29221 ctgacgaggc cgtgatggag gctatccggg atgtacttga agacggcgat ccccgcaccg
    29281 ccgggctggc tgaacagctg gcgaacggct atgtggatct gctcgacggt ctgccgttcg
    29341 gggaatcccg tgaataccgc gtcaccttcc gcgaactgac ggcgaaagac agcattgacg
    29401 cggaaaccga ggccgagcgt cttatggaaa cccgaaatgg cggcccggtt ctgatagcct
    29461 cgccggcact gcgcggcatt gcactgctgc gccgtcagat tgccgctgtc ggccagattg
    29521 aggggccatt gtctccgcgt cagatcggac agctcagcga gcgcgatctc tcccgtctga
    29581 tggcggcggt aagcctgctt gattccgcga tggcaggaaa gctggtcgct gaacgggggc
    29641 gaccagatgc agtgccggga tcagattgaa gaagccgcaa ttgtgctggg gatggtgaca
    29701 aaaagcggcc cggagtgggc gcttaacctc cccttatcgc agctttaccg gcactgccga
    29761 cagaccgaaa aaattctcag aacgaagcag taacccatgg caaaaaacct gaaagcctcc
    29821 ctgattgtcg atttactcgg caatatttct gccaaatccc gccagtggtc acaggagctg
    29881 ggggcattct cccgttccgg caggacgggg ctgggtggtc ttggaaatgc agcacgtcgt
    29941 gccggtcagg agacagaact tgtcggctcc cgtatgcagc gaacgcttgc cggtgttcgt
    30001 ggcagtatcc gcacggtgac gtcagatttt gaccgccttc agggcagcat taccggcacc
    30061 atcgggcgga taagcaacct ttacgggatg ctggcgggag gggctgcggt ctacgggttt
    30121 aataaagcct ttatccgccc ggccgcagag atggagaact atattctgcg tctcaacgcc
    30181 attaatcatg gcgataaggc aaaaacggaa gcagtgaaag cctgggcggt acagaacgcc
    30241 aaagacacca cctgggggct ggcgggggtt atgcaggaat acgcctccag tcgcgggttt
    30301 ggcatgagtg acagggaagc ccgtcgcttt attaccatgc ttcaggatca gggcggctat
    30361 cacggctggt cactgtcaga ggcacagggt gcgtccctgc aactcaaaca gatgtttgcc
    30421 cgccagagta tccaggcagc ggacgccaat attctgaccg gatacgggat taacgtttac
    30481 cagttactgg ccgataagct gggtgtaaac cagaagatca tccgtgagaa aggcgagaaa
    30541 ggaaaactgg gaccggacag cattcgcctg ttatttcagg tgatggccga acaggcaaaa
    30601 ggtgcccaga aaaacgccat gaactcatgg acgggaatga cgtccatgat gggtgacgtc
    30661 tgggatcagt ttgcccgtga cgtgatggcg aagggaccgt ttgacagcct gaaaaagagc
    30721 ctgaaaggct ttctggatta tgccgatgcg gcacagaaaa gcggcttaca ggataagctg
    30781 gccacacaga cggcctcagc actgaatcag ggatttgagt atgccagaga tgctgcgacc
    30841 ggcttttacc gggccattca gaaagtcagg gaaacgcttc aggcactgcg cgacgccggt
    30901 tatggcgatg cgctggatcg tattggtcag ggtgcgcaga ccgccgcaaa gtacctgatg
    30961 tatatgtatc tcgcctcccg tgcgctgaag gtgctcaggt ttgcagggac gggggcatta
    31021 cgtctcggcg caaccccctt acgctatggc atggcgatga cctccgtgct gacgtcgccc
    31081 ttccgcaaac cgcagaccac tgtaccggga acgcagcccg gacgcgctgg ccggttcctg
    31141 aatttcctta ccggggtaaa tccggctgcc gttcagcccg tgctggtcac caactggcct
    31201 gcgggtggcc tggcctcagg tggcggggat gtggtggtca gcggtgacgg caaaaccgtg
    31261 cgcggccgta aaaaacgcgg ccccggacgt ggacggggtg tcacgaccgt cgtgactgcc
    31321 ggtgagcaac tggcggagag tgccggtaaa cagggcttct tcggtcgtat gatgagccgt
    31381 gccggtggtc tgctaacggc ggccggaaac cgcatggggc tggggcgttt tgccgggctg
    31441 ttccgtggtg ccggtcggct gggtggtggt gccctgtggg ccggtgccat ggcggctcct
    31501 gtactgctgg acagcagcgc cagtgcagcc gataaagccg gtgccgtggg ttctcttgcc
    31561 ggaagtatcg ccggtggcgc gctgggggcg gctgccggtc cggtcggggt tgccattggt
    31621 tccacggtgg gcagctatct cggtgattat ctggggggct ggctgacgca ggcctggcag
    31681 aaactgcgtg gcagcagtga cgaaaacggc ggacaggcca ccgcgaaaac cgccgcacgg
    31741 gtggagcttg tggcccctga aggctggcgg gcgcgcagta ttgatgtgga tgataccgca
    31801 cagcatggcc tggatgtgaa cgtctggaac ggagggaact atggcctcta ctgacacgcc
    31861 ggggcgtggc tcctttcgtg gcgtaccgtt cctggtttat caggagcagc gggaacgggg
    31921 cggacgtaac attgtgcgcc gtgaataccc actgcgtgaa agcggcggtg cggatgatct
    31981 ggggccaaaa ctcccggagt tcacattcac ggttctggtg accggagacg atcttcagac
    32041 acagcgaagc cgcctgcgtg atgcgctgcg tgcacccggt gccggtgaac tgatgcaccc
    32101 ggattacggc acgttaaacg tgctgataaa cagctttgaa agccgttata acgccagcga
    32161 gcagggaacg gttgagttca cgatcaacgt gatccccgcc agtgacgata ccgcccctgc
    32221 ggtggcagaa gatacggcag ctgcgctgga gcagaaaagc ggctcagcaa tgaatcagct
    32281 gtttaacacg ctttcagacg gctggacggt gatttcagac ggtctgcatg atgtacaggc
    32341 catgaccgac accatcagcg ataagattga tgcactggag aatgccgttt cggggatggg
    32401 gattgtgcag gatatcagtg cctttacggc cagttttact gccctgaaag gcaatgccgc
    32461 cgccctgatt aatgcgcccc gtcgcatggc tgaatcgctg gccggaatgt ttgccgtgct
    32521 gaccgggctg cccggcgatc cttccctgtc tctgaccgga aaagccggaa cgccgtccgg
    32581 cagcctgacc acaaaccgtg acagcatggc agagcaggcc atgccgcagc tgtaccgcac
    32641 actttcctcc ctgcgttaca ccctcagcga acaggatgat ccacagcgcc tgattggcct
    32701 gacgcctgcg gcacagaaaa atatccgtct gctgcggagc gtgatgcaga gtgcaaccct
    32761 ggtgtcgcag gcacagacgc tcggaaaact gctggatcag gtcatccggc agaatacgac
    32821 gcacgttcag gatactgacc gttcgggcgg actggcctgg ctggaaagtt ctgctgatgt
    32881 gcagcgtatc aaccgggatt tgagcgatgc catggaacaa caggtgctgg atctgtccgc
    32941 acagggctat accgggactg cccttgcatt acgtgatgca gggcttgcgc tgacagaaga
    33001 tttaaccaca cggagcgtgc atcttcccgg tgcttttcag gtcatggttc gcaccaccga
    33061 accggcactg gtcacgctgt accgtgccac cggcaacagc agtcgctggc agtattttgt
    33121 ccgcagaaac aatatccccg atccggtttt tgttccgggt ggccgcagcg tggaggtgat
    33181 cagtgagcag caggattgaa ctgtatatcg gtggcagtat tttttccggc tggctgacgg
    33241 tcagcgtccg tcgctcgctg gagcatctgg ccgggtcgtt tgaactgggg ctgatgctgc
    33301 ccggtgagcg catcccctca gccctgcgaa ccggtcagtc cctgacgctc agaattaacg
    33361 ggcagacggt catcagtggc tggctggatc aggtcagtca gcggatcagt gcaacccgtc
    33421 atcagatcag catcagcggt cgggataaaa ccggcgacct ggtggactgt gccgccatcc
    33481 atcccggcag ccagtggcgc aaccggacgc tggcacagat tgccgcagat ttatgtgcgc
    33541 ccttcgggat agccgtgcgc tggcaggtta acgacgacac ggctgcacgc cccttcagct
    33601 cttttacgct ggaaaactca gaaaccgtgg cggatgcgct gacccgtgct gcccggcatc
    33661 gcggggtgct ggtgaccagt aatgctgacg gcgatctggt gttcacccag gcaggaagcc
    33721 agcagacgga cagactggtg ctgggagata acctgcttga tgccgattac aacacggact
    33781 ggcgaggacg atacagcgaa taccgtgtcc ggggacacgg gcgcggtggt ggcaaacggg
    33841 gagacagcga atccgccgcc cgtctggcag cacctgtggg cgtcatcagt gatgagcaga
    33901 tcggccgcta ccggtcgaaa atcatcctcg ccgatcaaca gacagacacc accggtgcac
    33961 ggcagcgtgc cctgcgtgaa atgcgccgtg cgattgcccg ttcagaacgg ttttctgcca
    34021 ccgtgcgtgg ctggttccgg gatgatggcc ggttatggga tgtcaatctg ctgaccggtg
    34081 tttcagccct gcgtttcggt atagaacaga ctgaactgct ggtctgtcag gtggagtttt
    34141 tacttgatga acataacggg gaagtcaccc ggctggtact ggcaccgcgt gacggcttta
    34201 tcgttccggc agagccggac agtaagggcc ggggtggttc cggtgacgat gttgatgcct
    34261 ttattcgcca gcagatgaaa aaacagggga tcagctttga tgaatgatga agtgttcagc
    34321 cgcctgattg ccccggtaac gcgcggtatt cgcctgttgt ttggccgggg cgttctgacc
    34381 ggcacacatg acgaactgaa aatgcagaat gtgcagctca ccggcatgga cggcgaaacc
    34441 tttgatgatg tggagcgtcc ccagcagtac gggcagatca gcgttcccct gccgggtgcg
    34501 gaaacctttt ttgcctgtct gggcggacag cgggatcaga ctgtggtgct tgtggtggaa
    34561 gaccggcgca gccgcccgac cggactcaca gccggagata cgggggtgta tcaccatgag
    34621 gggcaccgga tacggttaac aaaggatggt cgcattattg tgacatgcaa aatgctggag
    34681 atttacgccg atgaggggat gcgggtggat acgccggagg ccacctttac gggcaatgtg
    34741 acggtggata aaaacctgca tgttaagggt aatttcgtgc ttgatggtac aggtaaatca
    34801 gacgggctgt tcacgatgtc tgatgccgtt attgccggga tcacctattc cggccatgtg
    34861 catcaggata acggcaaagg cagtaagaca ggagcgccgg agaatggctg atattgcaat
    34921 tgtatgggat caggggtgcg gttcgctgca actgaacggt gccgacttgc tgacggatga
    34981 cagtctgctg acggccgttc tgatttcgct gtttactgac cgccgggcgc tggcatcgga
    35041 tgaaatccct gacggtacgc gcgaccgccg gggatggtgg ggagacagtt ttcgcccgcg
    35101 ccccattggc tcccgtctgt ggcttctcag ccgggaaaaa acgctggcct cggtgataag
    35161 ccgtgcccgt gcttacgcgg atgaggcgct gggctggctc agtcaggatg gcgtggcgtc
    35221 atccgtggtc tgtcatgcag aacgtgtggg acacgcgcgg ctggcgcttt cggtgcgcat
    35281 aaccctgcct gatggttcag tgaggcccat gattttttat gctgatctta agggggagta
    35341 atgccttatc agcctttacc gctggcacag ttaatcacgc agacacagca ggatatcagc
    35401 cagcgcctgc ccggctcgca gccgggtgtg aatgaaacca ccctgaatgc cattgcttac
    35461 gctcaggcag ggttatctgc tcaggagcat gagcatctgg cctggattgc gcgtcagatc
    35521 atcccgaccg aagccgatga agccgaactg ctgaaacact gtgcattctg gggcgtcatg
    35581 cgtaagcctg cctctcgggg ggacgggccg gttcagctga tgctgaccac ggatgcgggg
    35641 atcaaggaag gtgtgcttct ccagcgaagt gatggcgtgg tttaccgtat caccacctcc
    35701 ctgaccggta aggccggtac gctgaatgtc agtgtggaag ccgaaagtgc cggtcgtgcg
    35761 gggaatgccc cggcagggac aaaactgaca tttatcacac cgcaggcggg gatcaaccag
    35821 acggccacgg tgaccggaac ggggatcacc ggtggtgcgg atgtggaaac cgtgccggag
    35881 ctgctttccc gtctggtttt ccgggtgcag aatccaccgt ccggcggcac gcagtatgat
    35941 tttgaacgct gggcgcgtga agtgccgggc gtgacgcggg catggtgccg cccggaatgg
    36001 ccgcaggcgg gcagcgtggg ggtaacgttt gttcaggata ataacccgga tattttcccc
    36061 ggtgacggtg atgttcagcg ggtggcggat tatatccgca gtcatgatga tccggcgacc
    36121 ggccagcctg tcggacagcc tctggggccg acggtgaccg tgtttaaact gaccaataag
    36181 ccggtgccct tcagcatcag gatcatcccg aaaacaccgg agaatcaggc ggccgtaaaa
    36241 caggcgctga ccgacctgtt gtataacgaa tcccggcccg gcggtctggt tctgccgtcg
    36301 tctttctggc gggcggtggc aggggtgaaa aatctggagg attttgaagt gcgcagtccg
    36361 ctgacgtcag ttcaggcggg agacagtgag ctgctgacgg taggagaaat cacatggctg
    36421 taaccctgac cccgcatcag cgcgccctgt tgcagttact gcctgacggg ctggcatggg
    36481 acaagcgtcc gtcatccgta ctggcatcat tatgtctggg cctcagtcat tcgacagcgc
    36541 gtgtatcctg gacgggtaaa cagctgcttg ccgagcgttt ccctgacacg tcgcgcctgc
    36601 tgctggaaga ctgggagcgt tatctcgggc ttcccgagtg tgatatggcc ggagccaccc
    36661 tcacggagcg acagcgttat gccggaaata aataccggat gaagccctcg ctgaaccgtg
    36721 aattttatat ccggtttgcg gcggagtttg gttatcagat tgacattcag ccctcaccgg
    36781 attcgcagtg ggtcagtatt gtcacgatta acagtgaaac cggctaccgg aatatgaatg
    36841 tgctggatga tattctcacg ccgctgcgta tttatgaagg cggtgcgctg gaatgtattc
    36901 tgaaccgtta taagcctgca tggcagacgt ttatttacgt ttatgcaaac agccatgaag
    36961 aggagactat ttaatgtttc atgttgataa taattccggc gtggcgaata tgcctgcgct
    37021 ggcaccggcg cagagtaata ccaccacctg gtttaccgaa ggtgacggac aaaaaggtat
    37081 cagctggatt ggtcaggact ggctgaatat tctccaggcc gaactgctga atattctggc
    37141 tgaagccagt attcagccgg ataaggcgca gttaaaccag cttacgctgt ccattaaagc
    37201 cattatcgct gcgaatgcct tttcccggaa aaataacctg aaagaaattg ctgatgccgg
    37261 tgcggaggcc cagcgtctgg cccgtggtta tcttggtctg gggacgcttg ccacaaaaaa
    37321 cagtcttggt cccgctgacg ttaatgccct ggcgaaggat cagaatctcg ccgacctgga
    37381 gaataaggga accgcccgta ataatctgga tgtttacagc aaaagcgaag gtgataaccg
    37441 ttacctgcgc agggagcaga acggcgcaga cattccggat aaaggggctt ttatcgataa
    37501 cgtcggttta cgggaaacgg taaacagggc ggcgaatgcc ctgccatcgg acggcaccgc
    37561 cgttgccgcg aacaggctgg caaatgccca cacaattaat ggtgttccct ttgacgggac
    37621 gcaggatatc actatctcat caggaacagt aaccgccata agactggggt cggtgaccgc
    37681 acatatgccc ggaacatggg aaagctggga tctgaatctg gggggcggca atgttcttac
    37741 cggaattaaa gtacaggatg tcggtaagaa tacggcagat aacgtcggcg gcgtgtatta
    37801 ccggccgttg cagtatctgt taaatggagc atgggtgacg gctgcaagta tataatgata
    37861 ttcagaaggc cttccggcct tctttattac tgtggctgct caggccattt aatggcgtta
    37921 aattcagctt ctgttttaat ggcgggtagt tccattttct tcacctgact gatgtatgcc
    37981 atccattggg taagtgctgc tctgtcttca tcgctgatgg tgcccagttg tagctccgtt
    38041 ctccagtcat taattttttc atatgcctga ttaagatatg actggcgcat tgcttccagt
    38101 tttcttgcgt agttaaccgg aatacgggag attatgccat tatcaaactt ccagtttcct
    38161 gaaatatccg tgccttcagg gagttcatcg acctcaacca cagaaaaccc cgcaggatac
    38221 agcgccgagg cgtcctgtga tattgagcag ataacgccgc tgtcaggtgt tatgcaaagt
    38281 ttatattttt tggtgaataa tggcagggat tcgtagaaat ccttaccatc ttcactctgg
    38341 aaatactgaa cattattacc atatggcata ttttcaggat aataacgggt tatatttttt
    38401 agttccataa aatcctcctg gaaataaatc tgttttaaaa tatattattt actgactatt
    38461 tataactgag ataccgtaat ccatgtacct ccgactaatt tttgcagggg tctgaatgct
    38521 ataaaatcgt ttgaagcatc aactttactg tcaatctgca ggcctgccat aacattaccg
    38581 tacgcagagt aatatgcccc accgtacatt acctgcctgt tctctgctcc cagcctgata
    38641 tcctggatat atcgggaatc cgcatcctgc tgtgttatac cagaagtgat gtttatgtcc
    38701 tgcgtcccgt caaagggaac cccattaata tttcttgggg tggcgagtct attcgcggca
    38761 acggcggtgc cgtccgatgg cagagcattc gccgccctgt ttaccgtttc ccgtaaaccg
    38821 aggttttcag gaaagccagg attaactctg ttttattcct catgcgacaa ttccggcttt
    38881 ttataataag gaaatcgtat gctgattggg tatatcaggg tgtcaacaaa tgaccagaat
    38941 acggcgctac aaagaaatgc gctggaatgt gcaggatgtg agctgttttt cgaagacaga
    39001 ataagcggta agacaaggga gcgtcccgga cttaaacgcg cattaaagcg actccggcca
    39061 ggggataccc tgctggtctg gaaactggat cgccttggac gcagtatgca gcaccttgtc
    39121 cttttaacag aagaactccg gcaacgcggc gttaacttcc gcagcattac ggacaatatt
    39181 gatacatcca ctcccatggg acgtttcttt ttccatataa tgggcgcgct ggcggaaatg
    39241 gagcgggaac tcattattga acgtacacgc gcgggtcttg ctgctgcccg tgctcagggg
    39301 tgtcatggcg gcagacctca gaaactgaca gagaaagagc gggagcaaat cgcccgtttg
    39361 ctggctaatg gcgagtcccg taaacaaatc gcactgatat ttgatattgg cctttccacc
    39421 ctttatcgtt attttcctgc ctcaccagct ccgtcaccaa aagtaccgtg acgctttttt
    39481 gaatttccgc aaaacacata tttttcagcg gtcgctattc tgactctgtt taattcagaa
    39541 acaggcagaa aagacgatgt cagactccaa gtggaaaatg ctctccgcca tgcccggaga
    39601 gttttcagtc aaggttgccg gtggcacggt ggcatttatt gaaagccctt tccgcccttc
    39661 gggtaataaa ggggggatca ccttcgcaga ctgcgtgatc cgcttcagca cgaaagagcc
    39721 attatgggta atgccggtat ccggtaatcc cagtgcagaa atcaccagtt caggcgtgac
    39781 tggcatcatt ccaatcacat ctgacgtggc cggaacgctc acgccctccg actggaatgc
    39841 accggataac gccgggcctt ccggtagcac gaatggcagc agttcagagc cggaatatta
    39901 ctttgttgtc attctcgcgg gtcagtcaaa tggcatggcc tacggcgagg ggctgccgtt
    39961 accggattcg tttgaccgcc cggacccacg cattaagcaa ctggcacgcc gcagtacggt
    40021 aacgcccaac ggtacgtcgt gtacatacaa cgacatcatt ccggcagacc actgcctgca
    40081 tgatgttcag gatatgagcg ggataaacca cccgaaagct gatctggcaa aaggccagta
    40141 cgggactgtc ggtcaggggt tgcatattgc caaaaaactg cttccctata tcccacagaa
    40201 tgccgggata cttctggtgc cctgttgtcg tggcgggtca gcattcacca ctggagccga
    40261 cggctcattc agtgaggcca gcggtgcttc cgctgattct tcacgctggg gggccggtaa
    40321 acctctgtat caggacctgg taagccgcac caaagtcgcg ctggcgaaaa acccgaaaaa
    40381 caaacttctg gctgtggtct ggatgcaggg agaggcagac cttgcctcag gaagccagca
    40441 gcataatagt ttattcacgg ccatggttca gcagttcaga actgacctgg ctccgcttgc
    40501 tgcgcagtgc gtgagtggaa atgccaccac ggtgccgtgg atttgcggtg ataccacgta
    40561 ttactggaaa aacgctggca ccgataaata tgaggcagta tacggtggct acaaaggcaa
    40621 ggaagcacag aaaattttct ttgtaccgtt cctgacggat gagaatggac agagcacgcc
    40681 aacgaatgct ccggcggaag acccggatat tgtggctgtc gggtattacg gtgcagcatc
    40741 ccgaacccag ggcagttttg tttcgacaca gcgtgacagc catttcagct catgggcacg
    40801 caggggcgtc atttcagacc gtctggcctc agctattatg ctccatgcag ggcgcacggc
    40861 tgaactgatg cgcgggcaga ccgtgacacc accggatgag aagccgtcac ctgatacacc
    40921 atcaaaaccg tccacgccac ccgcagacac cacgacgatg agtacgctat ttgcttaccg
    40981 ggcatctgag tctgaaggac ggttgacacc acagggttgg gctgccggag gtggcaaggc
    41041 tcaaatcgtg gatgatgccg gagccagcgg gggtaaggca atgaaactga ccaaggaaac
    41101 aggaagagcc ccctggtacc ttgagcatga tgctggtaat ggtgcggacc tgctgggtaa
    41161 aggtggtctt gtcagttgtc gttttaaact cgatggcgcg cttacggcta atcagtacgc
    41221 actggcgctg tactggccgg tttctgccct gccgcaaggg gttactctgg aaggaaatgc
    41281 cggtcataac ctgctggcat cgttctacgt acagagcgat gccacagacc ttaacgtgat
    41341 gtatcacaaa ggaaacacgg cgcagaacac gaagctgggg tcattcggcg catttaataa
    41401 cgaatggcat acgctgggct tccgttttgc cggtaacaac agtattgagg tgacgccggt
    41461 cattgatggt aaggacggga caccattcat gctgtcgcag tctccggtcg gcacgtttac
    41521 ggcagacaag ttgcgcgtga ccgatatcac taaaaatgcg acttacccgg tactgattga
    41581 cagtatcgtg gtggaagtga ataacgcgta agcaggataa aaaaatgccg ccggtaaggg
    41641 aaaacaagaa accggcggca aaaagatcgt tatatccaaa gcaaaaaatg cagtgcacct
    41701 ttttacctaa cagatattac cggtgtcaac accatgtcaa taattgggag ggataatgac
    41761 atttttacag cttattttat tgtatttctg cacggcagta tgtgcgttat atctggtgag
    41821 tggtggttat aaggccatca gaaattatgt tcgccgcaaa attgaggacg cagcagctga
    41881 aaaactcagt aaaacagcgc aggtaactcc gctcccaaaa gacccgaccc agccctgatt
    41941 gcagggcttt attcccgcat aaggaggata tacatgccag aaattaaagg caccgtcact
    42001 gaagacctgg taaaacaggc actgtattcc ggtgaagtaa ataagttgct gaaagcgcag
    42061 gtccgcaagg attttgaggc acaaatcgac acttacgttg atgaagtgct ggcaaaactt
    42121 attgggccag cagttgcggc cagtgatgct gataatgagc cgaaaaccga cgcgcagcca
    42181 gagcaaccag acccggctca gcccggaact gacagtacca tgatgtaaca atacaaaccg
    42241 gtggtgtcct ctgccggttt gacactaatg aaaacatctt tgccgctatc cagccatatg
    42301 taatcgctgg atagtatttc gtattcagaa atatagctgc ttatggagca gtcaatcctg
    42361 cttccccatc cgggcaggca aatgaaccgg tatcattagt ccgattcacg cttttgtaga
    42421 atcgttccca gaatgctata cagctcatta gcctgttgct ccatcattga gacggaggct
    42481 atacggtgca aagtgatttc aggaggtggc acacctccat cttcttgctg tggaggaaaa
    42541 acataagatt taataaaaaa gttgaagcat atgaactctc ccgcactcga actgtatttg
    42601 caggcatctg aataatcgga aaaataatca ttatagattt ccgcattttc aatcattttt
    42661 cgatttctcg tcgttacatg attgtccata gcagcctctt acgctggcaa ttaaccaatt
    42721 gacttctata aattagttga tttgtttgct ttgcggtatt caaatgtaca atcattcagc
    42781 acttatgcgt tacagcaccg tttaaaatta gtgatcaaac tcataaattt tcgctaatga
    42841 catttcgtgt tatttgttgt atcaattaaa taaatcaaat cgatcgtttt tgtcgatcga
    42901 ttgagtgata aagatatcaa caacaaagca ggaggttatg cggccatgta tcgaaacata
    42961 cggtgccgac attgtaacaa gctactggca cgagccagct tcagttatct ggaagttaaa
    43021 tgtccgcgct gtaaaaccct caatcaaatc acatctcaga gcgccacaga gcaccccaca
    43081 tacacaagga aatcctgtcg tggggaacag aaagcaagtt acatcccgta tcatcagcac
    43141 gcctgaactt attcactata acgacaatat cgtcggttat ggctcccgtg agttacgggt
    43201 tgagacaata agttgctggc tggcccggct ggtcgtcgtc aataaacatt acagccatcg
    43261 tttcgtaaat aattcctacc ttcatctggg catattttca gaacgggagc tggttggcgt
    43321 aatgcaatgg ggttatgccc ttaatccaaa caatggtgca cgcgtcgtaa ctggcacgca
    43381 gaaccgcgaa tatatggagc ttaaccggtt atggctgcat gactgtatgc cgagaaactc
    43441 cgaatcaagg gcaatcagtt acgcgctcaa actaatcaga caactttatc cgcaggtgca
    43501 gtgggttcag tcgtttgcag atgagcgttg tggctgtctt ggtgtcgtgt accaggcaag
    43561 caattttgat tatgtaggca gtcatgaaac aacattctat gaacttgatg gcgagtggta
    43621 tcacgagatt tgcaagaatg caatcaaacg aggaggacag cgaggtgaac atctgcggac
    43681 taatatcgac agagccagtg ttcataaatt ccgtcagttt cgttacatcc ggtttctgga
    43741 caagagagcc agaaaacgcc ttaacacaaa gctattcaaa gtccagcctt acccaaaacc
    43801 acaaacagtt aaaactggtt tgaaagagag tgaatgaacg cttaaaaact cattctaaaa
    43861 ctcaaaagaa aagcgccgat ttagtatcaa ataagttgcg tatcggcgtt tttcagcggt
    43921 atcaaatgaa atgcaaatcg gtatcaaaaa aatcgccgcg ctataatttg tcgccgtcag
    43981 tacagcatgt accggaactt taggtttttt tcgttgagcg tcgcgaatag cttcactgtg
    44041 tggacgtgct gcatgaacag ggcggctgtc taaaatcatc aaggtctaaa tacactatga
    44101 ggttgattag gggttggtac aagcccaact aaccgaatca actatgagga tgattcctag
    44161 aacatccgaa tcaactacct gtaaaaacag gacaatatca ggtgaatgac tatgcaattt
    44221 aacggtgttt ttccaagtat ctatgcaatc ctacacaaat tctatgcagt atcggtatag
    44281 tctcttttta gcataactta ttgaaatata taaagaatct gtgaagtctc ttttcagggt
    44341 aaactgtatc gtgttagtcg tccctctcca gacactacat atagtgctat atacagggct
    44401 atataacgat atatagatag ttggtcacat ggtgtaacta tcaagaaact taacgatttc
    44461 ctacgtgaaa gttagaaagt gatggttata gttaccctcc tgaatcttca tagcgctcta
    44521 ttaaccagtc tcataaggaa gtccttttga gacactttca acgtaacggt agaatgcacc
    44581 ctcaagtagt ctcaaaaggt tgtttttgga ttatgagaca gctaagctta tgagactaac
    44641 ttttgagact tctaagggga acattatggc gatacttgga tattgcaggg tgagtacaga
    44701 tgaccaatcc atcactaacc agcaaatgca aattgaagag gcagggtaca acattgctaa
    44761 atggttcgct gatgaagctg tcagtggttc tgtcaaagct tctttaagaa acggtttcag
    44821 cagtttgtta gcttatgcac gagaaggtga caccgtagtt gtggtagctg tag
//
LOCUS       DAJTVK010000019        43715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000019 DAJTVK010000000
VERSION     DAJTVK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.019"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..561)
                     /locus_tag="OO372_001128"
     CDS             complement(<1..561)
                     /locus_tag="OO372_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR6340694.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRD"
     gene            complement(764..1000)
                     /gene="feoC"
                     /locus_tag="OO372_001129"
     CDS             complement(764..1000)
                     /gene="feoC"
                     /locus_tag="OO372_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6340695.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(1000..3321)
                     /gene="feoB"
                     /locus_tag="OO372_001130"
     CDS             complement(1000..3321)
                     /gene="feoB"
                     /locus_tag="OO372_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6340696.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3338..3565)
                     /gene="feoA"
                     /locus_tag="OO372_001131"
     CDS             complement(3338..3565)
                     /gene="feoA"
                     /locus_tag="OO372_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6340697.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4003..6324)
                     /locus_tag="OO372_001132"
     CDS             complement(4003..6324)
                     /locus_tag="OO372_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6340698.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6421..6897)
                     /gene="greB"
                     /locus_tag="OO372_001133"
     CDS             complement(6421..6897)
                     /gene="greB"
                     /locus_tag="OO372_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6340699.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7125..7844
                     /gene="ompR"
                     /locus_tag="OO372_001134"
     CDS             7125..7844
                     /gene="ompR"
                     /locus_tag="OO372_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6340700.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7841..9193
                     /gene="envZ"
                     /locus_tag="OO372_001135"
     CDS             7841..9193
                     /gene="envZ"
                     /locus_tag="OO372_001135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6340701.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9269..10891)
                     /gene="pckA"
                     /locus_tag="OO372_001136"
     CDS             complement(9269..10891)
                     /gene="pckA"
                     /locus_tag="OO372_001136"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6340702.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11270..11584
                     /locus_tag="OO372_001137"
     CDS             11270..11584
                     /locus_tag="OO372_001137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6340703.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11597..12970
                     /locus_tag="OO372_001138"
     CDS             11597..12970
                     /locus_tag="OO372_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6340704.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13105..13983)
                     /gene="hslO"
                     /locus_tag="OO372_001139"
     CDS             complement(13105..13983)
                     /gene="hslO"
                     /locus_tag="OO372_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6340705.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14008..14409)
                     /gene="hslR"
                     /locus_tag="OO372_001140"
     CDS             complement(14008..14409)
                     /gene="hslR"
                     /locus_tag="OO372_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6340706.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14420..15088)
                     /gene="yrfG"
                     /locus_tag="OO372_001141"
     CDS             complement(14420..15088)
                     /gene="yrfG"
                     /locus_tag="OO372_001141"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6340707.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15153..17288)
                     /gene="igaA"
                     /locus_tag="OO372_001142"
     CDS             complement(15153..17288)
                     /gene="igaA"
                     /locus_tag="OO372_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6340708.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17608..18168
                     /gene="nudE"
                     /locus_tag="OO372_001143"
     CDS             17608..18168
                     /gene="nudE"
                     /locus_tag="OO372_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6340709.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18333..20885)
                     /gene="mrcA"
                     /locus_tag="OO372_001144"
     CDS             complement(18333..20885)
                     /gene="mrcA"
                     /locus_tag="OO372_001144"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6340710.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21005..21784
                     /gene="hofM"
                     /locus_tag="OO372_001145"
     CDS             21005..21784
                     /gene="hofM"
                     /locus_tag="OO372_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6340711.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21784..22323
                     /gene="hofN"
                     /locus_tag="OO372_001146"
                     /pseudo
     CDS             21784..22323
                     /gene="hofN"
                     /locus_tag="OO372_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22307..22747
                     /gene="hofO"
                     /locus_tag="OO372_001147"
     CDS             22307..22747
                     /gene="hofO"
                     /locus_tag="OO372_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6340712.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22737..23141
                     /gene="hofP"
                     /locus_tag="OO372_001148"
     CDS             22737..23141
                     /gene="hofP"
                     /locus_tag="OO372_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR6340713.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23053..24291
                     /gene="hofQ"
                     /locus_tag="OO372_001149"
     CDS             23053..24291
                     /gene="hofQ"
                     /locus_tag="OO372_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6340714.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24692..25213
                     /gene="aroK"
                     /locus_tag="OO372_001150"
     CDS             24692..25213
                     /gene="aroK"
                     /locus_tag="OO372_001150"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6340715.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25270..26358
                     /gene="aroB"
                     /locus_tag="OO372_001151"
     CDS             25270..26358
                     /gene="aroB"
                     /locus_tag="OO372_001151"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6340716.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26450..27736
                     /gene="damX"
                     /locus_tag="OO372_001152"
     CDS             26450..27736
                     /gene="damX"
                     /locus_tag="OO372_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6340717.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27843..28679
                     /gene="dam"
                     /locus_tag="OO372_001153"
     CDS             27843..28679
                     /gene="dam"
                     /locus_tag="OO372_001153"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6340718.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28697..29374
                     /gene="rpe"
                     /locus_tag="OO372_001154"
     CDS             28697..29374
                     /gene="rpe"
                     /locus_tag="OO372_001154"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6340719.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29367..30125
                     /gene="gph"
                     /locus_tag="OO372_001155"
     CDS             29367..30125
                     /gene="gph"
                     /locus_tag="OO372_001155"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6340720.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30118..31122
                     /gene="trpS"
                     /locus_tag="OO372_001156"
     CDS             30118..31122
                     /gene="trpS"
                     /locus_tag="OO372_001156"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6340721.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31282..32187
                     /locus_tag="OO372_001157"
     CDS             31282..32187
                     /locus_tag="OO372_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340722.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32204..32566
                     /locus_tag="OO372_001158"
     CDS             32204..32566
                     /locus_tag="OO372_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6340723.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32650..33813
                     /locus_tag="OO372_001159"
                     /pseudo
     CDS             32650..33813
                     /locus_tag="OO372_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33813..35039
                     /locus_tag="OO372_001160"
                     /pseudo
     CDS             33813..35039
                     /locus_tag="OO372_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35036..35914
                     /locus_tag="OO372_001161"
     CDS             35036..35914
                     /locus_tag="OO372_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6340724.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35925..36278
                     /locus_tag="OO372_001162"
     CDS             35925..36278
                     /locus_tag="OO372_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6340725.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36290..37594
                     /locus_tag="OO372_001163"
     CDS             36290..37594
                     /locus_tag="OO372_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6340726.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37606..38691
                     /locus_tag="OO372_001164"
     CDS             37606..38691
                     /locus_tag="OO372_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6340727.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38843..39574)
                     /gene="phoB"
                     /locus_tag="OO372_001165"
     CDS             complement(38843..39574)
                     /gene="phoB"
                     /locus_tag="OO372_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6340728.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39674..40459)
                     /gene="frlD"
                     /locus_tag="OO372_001166"
     CDS             complement(39674..40459)
                     /gene="frlD"
                     /locus_tag="OO372_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6340729.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40456..41287)
                     /gene="frlC"
                     /locus_tag="OO372_001167"
                     /pseudo
     CDS             complement(40456..41287)
                     /gene="frlC"
                     /locus_tag="OO372_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41337..42359)
                     /gene="frlB"
                     /locus_tag="OO372_001168"
     CDS             complement(41337..42359)
                     /gene="frlB"
                     /locus_tag="OO372_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6340730.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42380..>43715)
                     /gene="frlA"
                     /locus_tag="OO372_001169"
     CDS             complement(42380..>43715)
                     /gene="frlA"
                     /locus_tag="OO372_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR6340731.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11128 c        11989 g        10441 t
ORIGIN      
        1 gtcgcgttga cgaatatgtt tttgaattaa ctccagcagc gccatacgtc ggtgctgcat
       61 gatttcatca tcaggcatga cggtgacatc aatcagcgga aatgcggagg cataaagctg
      121 ccttgccagt ttgggatcgg cgaaacaatc cagccaacac agcgaatagg gataggggct
      181 ttcaataccg tggtaaaaca acaatagcac caccatcggc aacgttttgt atccagcatc
      241 aaggtgattt tgcattgcgg caatagcgta acgcatcatg cgaaatgcga tcagtttgtt
      301 tgaggtgctt tgatgttcaa tcagacaata gatgtatcct ggtccttgtt ccgttttcac
      361 cgaccacagc acatcggaat agctttcacg cagatcgtca tcaataaagc tgctcgactc
      421 cagcttgagg gttttcatat cacaaagcgc gtgaatgggt gccggtaaat gaaacgcaag
      481 aaaatcacga gccgtgtccg gttggcgtaa aaagagtttg aacagcgcat cgtgtggggt
      541 ggaactctgc tttttgctca tggcgttcct tcacctgttg tttgaatgcc gcgactctac
      601 gcgccactcc acacctgtgc attagcgagt tttcatccct gcgagatgct ttcaaaggaa
      661 cttacgccgt gctgcaagag ttgcattttt atggaaagcc gcacgcaaaa aagcccggat
      721 gcagacgcat accgggcttt ccaaaacagg cgatggagta aggttaacgc agcgcccacc
      781 actcgcgcag acaggctttt ccttccgggc agcttttaca actgccagag aggcagccgt
      841 caggttcttc ctgaatccgc acggctttgc ccatactttc cagttgttgc agcatggcgt
      901 taatcattgg ctgcggagtg ttcaatgtct ggcttatctg ggtcgcttcc atacggcccc
      961 gtaacgccag caaatcacgt acctgaataa gtgaagccat taatggcaat caccggtggt
     1021 gctggctgcg cagcaactgc ttaccgactt gcgggttgcc agcagttcga tatccacccg
     1081 gctacgcgcg cggcgcagca gaccgataac tacgatgtta aacaggatta ccgccagaat
     1141 gcacaccagg ctgtaagttg ggtgctgact gtagctggcg acctgataga acaatgttgc
     1201 cagtgagtaa gcgatattca gcccccacag gatggagaag cccatccagc cacggcttga
     1261 ttcacgggcg atggctccca tcaccgagat acatggtaca tacagcagga cgaaaatcag
     1321 gtagctgtat gcggcagctg cgctaccgaa tttctgatcc atcacgccca tcgccccggt
     1381 acccatttca ccgtcgcctt tgctagcttc aatggggttc atcagtacgc taaggctgaa
     1441 ggtgtctttc aggctctgcc aggtttcatc taccgcactg aacagctctt caccgaggtt
     1501 gaattccgcc ggattgaact cttcgtcctg aatattttct gcggtgtaga gggtgttgag
     1561 cgtacccacc accacttctt ttgccattgc gcccgtaaac aggccaaccg ttgcctgcca
     1621 gttatcttca tgtacgccaa tcggcttgaa gacaggggtt atcacccggc tgacggacgc
     1681 cagcgccgag tcgttgatgt tatcgacgat tttcccgctc aacgagaagc tgttgaaagc
     1741 gctcaggaaa atgctgacga tgataatcac tttaccagca cgcagaacga agcccttcag
     1801 acgctgccag gtctggataa tcaggctttt aacgtgtggt acatggtaga ccggcagctc
     1861 catgacaaac ggcgtcgctt caccgcgcat gatggtgtac ttgagcatca ggccagtcag
     1921 caccgccatc acgataccga gcatatacag cgagaaaacc gccagggcac cgttctgccc
     1981 gaagaaggca gccgcgaata ctgcgaagat agccagacgc gcgccgcagg acataaacgg
     2041 tgccatcatg atggtcatca gacgttcacg cggtgcatca agcgtacgtg cgcccattac
     2101 cgacggtacg ttacaaccga aaccgacgat cagcggcaca aaggatttcc ccggcaagcc
     2161 cagcgcctgc atcaggcggt ccatcacaaa cgctgcacgc gccatatagc cggagtcctc
     2221 aaggaaggag aggaacaggt acatcatgcc gatctgcggc accagtggca gcacggtgtt
     2281 aataccgcca cccaggccct gggcgaggaa gatagtcagc cagtccggga agtggagcgt
     2341 gtagccaatc cattgaatac catgcacaaa tagcgccacg gagccgacgt caaacagcgg
     2401 ctgtaacgcc ccgccgatgt tgatagccag caggaacatc aggtacatca caaagaggaa
     2461 aatcggcaga ccgaggaaac ggttgagcac gattttatct accgcagtgg tgaaacggct
     2521 gggttctgcc gtcagggtgt tgcttaccac atcacagatg gcagctatgc actggtaacg
     2581 cgcatcggca atgtgcagcg ccggatcatc catttcatta cgcagacggg caagggcggc
     2641 atccagatgc tgcgacgctt caccggcgta ggcgcggctg tagatatcgc cttccagcat
     2701 ttgcaggccc agccagcgac gttgtttcag cgggatgtcg gaaggcatca cttttgccag
     2761 tgaatctgct tcgttgagta gcggctgtgc gtaatgcacc agttccacat tctcgttagc
     2821 tttatagcga tcaatcgcca gcttgagcgc ttcaataccg cgaccacggg ttgaaaccag
     2881 cgggatcact gggcagccaa gacgcgccga cagagcatca atttcaatac gaatattttg
     2941 cttctcggca atgtcgagca tgttcagtgc cacgatgcag ggaatgccga gttccagcag
     3001 ttgtagcgtc aggtacaggt tacgctcaag gttagacgca tccaccacgt taatcagcag
     3061 gtcggcgtcg ccactcaaaa tgtagtgaca ggcgatttgc tcatcgagcg aggtctgcga
     3121 tgagatggtg gtcagagaat aggtgccggg caggtccacc agcgtgacct gatgatcggt
     3181 ggtggagaat tgcccttctt tacgttcgac ggtaacgcca gcccagttac ctacacgctg
     3241 acgtgcgcta gtgagctggt taaataaggt tgtcttgcca gaatttggat taccaattaa
     3301 gccaatggtt aattttttca ttgttgttat caccgtatta acaggaaacc gcttccactt
     3361 ctaataaggc cagatctttt ttgcgtaata ccaggctcac acgacgggtt tcgatatgaa
     3421 tggggtcgcc gagtggagcg acgcgcacca cattaaaaga ggagccaggt aacatgccaa
     3481 gagaaagcag tttttggcga tatgccgggc tgatttcacg ggaaaagcca gtgattttcc
     3541 acgcagtatc tggagtgtat tgcataggtg cctacttgtt tctcattaac tggataacta
     3601 cctcatgggg cgtttgacag cgacatgaga ccagatgatt tgccaacaaa ggagagatta
     3661 aataatcaac aacacaatga taatgagaat ggtttctatc agcaatactt aaaatgtgtg
     3721 cgaatgttgg tttgaataat aatctgactc aatgttgatg tggctcaagg ttatatcgca
     3781 gcgaaaatat aattcgaata gtgaattacc cggactggct aatgtttaat taaggtttca
     3841 gttgttaatg aagtgataaa tattgccgga atatattatg agtttggttg tggaataaaa
     3901 aggccggaga tattctccgg cacttttatg tcaggcaagg cgttcgttgc cagatgcggc
     3961 gtgaacgcct tatcgcgcag gtttaggggc cagtccgggc gtttaacgtt ttttgcccat
     4021 tgccgccgcc agcgcatcca tcatcgcgct attaccggca ggctgcgctt cacgaccgcg
     4081 tggtttggct gccgggcggt tgttttgcgg gcgttcatta ccgccgccgc gacgggcgtt
     4141 ggtttcgcca ggctgctcgt ccagacgcat ggtcagggcg atacgtttac gctgaagatc
     4201 cacttccagc actttcacct tcacaatgtc gcccgctttc accacggtat gcggatcttc
     4261 cacaaacttg ttcgacaatg aagagatgtg aaccaggccg tcctgatgca cgccaatatc
     4321 gacaaacgcg ccaaagttgg tgacgttggt gacagcgcct tcgaggatca tacccggttg
     4381 caggtcgttc attgtctcga cgccatcggc aaactgagcg gttttaaatt ccggacgcgg
     4441 atcgcgaccc ggtttttcca gctctttgat gatgtcagtt actgtcggca caccgaattt
     4501 ttcatcagta aagtcagacg ctttcaggtt acgcagttcg ctgctgttac ccatcagatc
     4561 tttcagtgcc tgctgtgttg ctgccagaat gcgttccacc accggatagg cttccgggtg
     4621 aacggtagag gcgtccagcg ggttatcacc gtggttaatg cgcaggaagc ccgcgcactg
     4681 ctcgaaggct ttcggcccca gacggctgac tttcagcagt tgctgacggt tcttgaactg
     4741 gccgttctca tcgcgccagg ccacgatgtt ttgcgccatc atgcgcgtca ggcccgccac
     4801 gcgagttaat agcggaacag aagcggtgtt gagatcgacg ccaacggcgt ttacgcagtc
     4861 ttctactact gcatccagtt tgcgggccag ttgcgtctgg ctgacgtcat gctgatactg
     4921 acctacgccg atagatttcg gatcgatttt caccagctcc gccagcggat cctgcaaacg
     4981 gcgggcgata gataccgcac cacgcagcga aacgtcgaga tccgggaact cctgcgctgc
     5041 cagctcggaa gctgagtaaa ccgacgcgcc agcttcgctg acgatcactt tctgtgcggt
     5101 cactttcggg aactgcttct gcacgtcgag atagaaacgt tcagtttcgc gggaagctgt
     5161 accgttgccg atcgccacca gttcaacgtt atgtttttca cacaaggcag caacggtcat
     5221 cgctactttt gcggcttgtc cggtgtgcgg gtaaatggta tcggtcgcca ccagtttgcc
     5281 agtggcatcg accactgcca cttttacccc ggtacgcaga cccggatcga ggcccatcgt
     5341 tgcacgcagt ccggcagggg ccgccatcag cagatcgtgc aggttacggg caaagacgtt
     5401 gattgcttca tcttccgcgc gttcgcgcac ggtacccatc agttcggttt ccagatgcat
     5461 cagcaccttg atgcgccagg tccagctcac tacgcctttg cgccagctat ccgccggggc
     5521 attgttcagg cgcaggccaa ggtgatccat gatgatttgc tcgcaatagc tctctttggg
     5581 cggctcatcg aactgcggat cggcattcag cgaaagctgg agtacgcctt cgttacgccc
     5641 acggaacatc gccagcgcgc ggtgagaagg caccgtggac aacggttcgt gatgatcgaa
     5701 atagtcgcgg aatttcgccc cttcctcttc tttaccgctc accaccgtag aaaccaaatg
     5761 cgcgttcttc cacagataat cacgcacttt cgccagcagc gcggcatctt cggcaaaccg
     5821 ttccatcagg atatagcgcg cgccgtccag cgcggctttg gtatctgcca cgcctttatc
     5881 ggcatcaata tattgcgcag cggcgacttc tggtgtgtgt gacggatcgc tccacagcag
     5941 gtcagccaac ggctcaagcc ctgcttcaat ggcgatttgc ccgcgggtgc ggcgtttagg
     6001 tttgtagggc aggtagaggt cttcgagttc ggttttgctt agggtggcgt tgatggcctt
     6061 cgccagatca tcggtgagtt tgccttgctc ggaaatggac ttgaggatcg cctgacgtct
     6121 ctcttccagc tcgcgcagat agctcagacg cgtttccaga ttacgcagct gcgtgtcatc
     6181 cagaccgccg gtgatttcct tacgataacg tgcgataaac ggcacggtat tcccttcgtc
     6241 aagcaggcga acggcagcgt caacctgttc cgggcgcgcc tgaatttcac ccgcaataat
     6301 gcggcagaac gaatcattca tcatggtttg agttcatctt ttcggatcaa aaatcagggg
     6361 atagttatac ggactggctg gcaaaaatgc cagccatcgg caggaggtta agactcttca
     6421 ttacggtttc acgtattcga tagcattaac ataccagctc gcttccccgg caggggtatt
     6481 caccaccgcc agatcgccga cttctttttt cagcaatgcg cgggccatcg gggaatcgat
     6541 agagatgtaa tctttacggc caaaaatttc atcgtagccg acaatacgga aacggtgagt
     6601 cacgccatcg tcgttttcaa tctccaccca cgcgccaaaa aagactttgc cttcctgctg
     6661 aggggagtaa ttgacgattt tgagattttc catgcattta gtgagatagc gcacgcgacg
     6721 gtcgatttca cgcagacgct ttttattata ctgatagtca gcattttcgc tgcggtcgcc
     6781 cagacttgcg gcccaggtca ccttttttgt gacctccggg cgttcttcac gccagagata
     6841 attaagctct tgtttgagtt tttcataccc ttcccgggta accaggggcg ttttcatctc
     6901 gttgattccc tttgtctgtt tgataatgcg cacattgggt ataacgtgat catatcaaca
     6961 gaatcaataa tgtttcgccg aataaattgt atacttaagc tgctgtttaa tatgctttgt
     7021 aacaatttag gctgaaattc ataccagatt tagctggtga cgaacgtgag cttttttaag
     7081 aatacacgct tacaaattgt tgcgaacctt tgggagtaca aacaatgcaa gagaactaca
     7141 agattctggt ggtcgatgac gacatgcgcc tgcgtgcgct gctggaacgt tatctcaccg
     7201 aacaaggctt ccaggttcga agcgtcgcta atgcagaaca gatggatcgc ctgctgactc
     7261 gtgaatcttt ccatcttatg gtactggatt taatgttacc tggtgaagat ggcttgtcga
     7321 tttgccgacg tcttcgtagt cagagcaacc cgatgccgat cattatggtg acggcgaaag
     7381 gggaagaagt ggaccgtatc gtaggcctgg agattggcgc tgacgactac attccaaaac
     7441 cgtttaaccc gcgtgaactg ctggcccgta ttcgtgcggt gctgcgtcgt caggcgaacg
     7501 aactgccagg cgcaccgtca caggaagagg cggtaattgc tttcggtaag ttcaaactta
     7561 acctcggtac gcgcgaaatg ttccgcgaag atgagccgat gccgctcacc agcggtgagt
     7621 ttgcggtact gaaggcactg gtcagccatc cgcgtgagcc gctctcccgc gataagctga
     7681 tgaaccttgc ccgtggtcgt gaatattccg caatggaacg ctccatcgac gtgcagattt
     7741 cgcgtctgcg ccgcatggta gaagaagatc cagcgcatcc gcgttacatt cagaccgttt
     7801 ggggcctggg ctacgtcttt gtaccggacg gctctaaagc atgaggcgat tgcgcttctc
     7861 gccacgaagt tcatttgccc gtacgttatt gctcatcgtc accttgctgt tcgccagcct
     7921 ggtgacgact tatctggtgg tgctgaactt cgcgattttg ccgagcctcc agcagtttaa
     7981 taaagtcctc gcgtacgaag tgcgtatgtt gatgaccgac aaactgcaac tggaggacgg
     8041 cacgcagttg gttgtgcctc ccgctttccg tcgggagatc taccgtgagc tggggatctc
     8101 tctctactcc aacgaggctg ccgaagaggc aggtctgcgt tgggcgcaac actatgaatt
     8161 cttaagccat cagatggcac agcaactggg cggcccgacg gaagtgcgcg ttgaggtcaa
     8221 caaaagttcg cctgtcgtct ggctgaaaac ctggctgtcg cccaatatct gggtacgcgt
     8281 gccgctgacc gaaattcatc agggcgattt ctctccgctg ttccgctata cgctggcgat
     8341 tatgctattg gcgataggcg gggcgtggct gtttattcgt atccagaacc gaccgttggt
     8401 cgatctcgaa catgctgctt tgcaggtcgg taaagggatt attccgccgc cgctgcgtga
     8461 gtatggcgcg tccgaggtgc gttccgttac ccgcgccttt aaccatatgg cggctggtgt
     8521 taagcaactg gcggatgacc gcacgctgct gatggcgggg gtaagtcacg acttgcgcac
     8581 gccgctgacg cgtattcgcc tggcgactga gatgatgagc gagcaggatg gctatctggc
     8641 agaatcgatc aataaagata tcgaagagtg caacgccatc attgagcagt ttatcgacta
     8701 cctgcgcacc gggcaggaga tgccgatgga aatggcggat cttaatgcag tgctcggtga
     8761 ggtgattgct gccgaaagtg gctatgagcg ggaaattgaa accgcgcttt accccggcag
     8821 cattgaagtg aaaatgcacc cgctgtcgat caaacgcgcg gtggcgaata tggtggtcaa
     8881 cgccgctcgt tatggcaatg gctggatcaa agtcagcagc ggaacggagc cgaatcgcgc
     8941 ctggttccag gtggaagatg acggtccggg aattgcgccg gaacaacgta agcacctgtt
     9001 ccagccgttt gtccgcggcg acagtgcgcg caccattagc ggcacgggat tagggctggc
     9061 aattgtgcag cgtatcgtgg ataaccataa cgggatgctg gagcttggca ccagcgagcg
     9121 gggcgggctt tccattcgcg cctggctgcc agtgccggta acgcgggcgc agggcacgac
     9181 aaaagaaggg taaataaacg ggaggcgaag gtgcctcccg ttttgctttc tataagatac
     9241 tggatagata ttctccagct tcaaatcatt acagtttcgg accagccgct accagcgcgg
     9301 cacccgcagg ggtgtcggtg tatttatcga agttgtcgat aaacagtttc gccagggttt
     9361 cggctttttc ctgccactgt tccggagaag cgtaggtgtt acgcggatcg agaatcttcg
     9421 tgtctacgcc cggcagttcg gttgggatcg ccaggttaaa catcggcaga gtgaaggttt
     9481 ctgcattatc cagcgaaccg ttgaggatgg cgtcgataat ggcgcgggta tctttaatcg
     9541 agatacgttt gccagtgccg ttccagccag tgttaaccag ataagcctgc gcgcccgccg
     9601 cctgcatacg tttcaccagc acttctgcgt actgagtcgg gtgcagcgac aggaacgccg
     9661 cgccgaagca agcggagaag gttggcgtcg gttcggtgat gccacgctca gtaccggcca
     9721 gtttggcggt gaagccagag aggaagtgat actgggtttg atcggcagtc aggcgagaaa
     9781 ccggcggcaa cacgccgaaa gcatcagcag tcaggaagat aaccttagtc gcgtggcccg
     9841 ctttggaaac cggcttaacg atgttatcga tgtgatagat tggataagaa acgcgggtgt
     9901 tctcggtttt tgaaccatca tcaaagtcga tagtgccatc ttcacgcacg gtgacgtttt
     9961 ccagcagcgc atcacgacgg atagcgttgt agatttcagg ttccgcttct ttcgacagct
    10021 tgatagtttt tgcgtagcag ccgccttcga aattaaacac gccgtcatcg tcccagccgt
    10081 gttcgtcatc gccaatcagg cggcgtttcg ggtcggtgga aagggtggtt ttaccggtgc
    10141 cggaaaggcc gaagaacacc gcaacatcgc ctttctcgcc aacgttggcg gagcagtgca
    10201 tagaagcgat acctttcagc ggcagcaggt agttcatcat cgagaacatc cctttcttca
    10261 tttcgccgcc gtaccaggtg ccgccaatca gctgcatgcg ctcggtcagg ttaaacgcca
    10321 cgaagttttc ggagttcaga ccctgttctt tccactgcgg gttagtgcac ttcgcgccgt
    10381 tcataacgat aaagtctggt ttgaaacctg ccagctcttc atcgctcggg cgaataaaca
    10441 tgtttttgac aaaatgcgcc tgccaggcca cttcggtgat gaaacggacg gaaagacgag
    10501 tatccgggtt cgcaccacag aaagcgtcga caacgaacag acgtttgccg gaaagctgcc
    10561 tggtcaccag gcctttcaga tgctgccagg tttccggaga gagaggtttg ttgtcgttct
    10621 tacctttgcc tttgtctgcc caccagaaag tatcgcgagt ggtatcgtca cggacgatat
    10681 acttatcttt tggtgaacga ccggtgaaga tcccggtatc gacggcaacg gcacccagat
    10741 tagttaacac cccgcgctca taacctgtca ggctcggatc gagctcttcc tgatacagca
    10801 ggtcgtagct tgggttgtaa acgatatcat gtacgtcact gataccataa gcctcgagtt
    10861 cttgcggggt caaaccattg ttaacgcgca tttcactgct ccttagccaa tatgtattgc
    10921 ctgaatagta aagtcttttt gggggtgtta accgcgacaa ggctcataga tttacgtatc
    10981 tggagaaatt catatttgac aaaaaactcc gtgattcctg tcacgaaacg gttgctatta
    11041 tcgcagaaat agcattttag gtgtggaaaa tgttctcaaa aggttaaatc ttggtttata
    11101 tggcgggaat attgtgagtg gaatcgcatt cctgaatgaa attgatgcaa attcaaccgg
    11161 taaagtatcg attcaccacg ctaacttttt ttgattattt tctgaaaaaa gcaaaatact
    11221 actgcccgga tgcgataatt aacccacctt ttgattctgg ataaccaaca tggataacgt
    11281 tgaactttca ccggcaacac gctgggggat gattgctacc ggattacttc aggggctggt
    11341 ttgctacctg cttatcgcct ggcttgccgg aaaaaatcac agttggattg tttatggcgt
    11401 gcctgcgacc gtcgcgtttt catccgtttt actcttctcc gtgatctctt ttaaacagaa
    11461 gcgcctttgg ggatggctgg cgctggtgtt tattgccacg acagggatga gcggttggct
    11521 gaagtggcaa actgatggca tgaacccctg gagagctgaa aaggcgattt gggattttgg
    11581 ctgatatctg ctgttgatgg caatgttgtt gctaccgtgg atacaacaaa gccttcgtat
    11641 ccgcaatggc agtagccgct atagctactt ttaccagtca gtatggcata acgtacttat
    11701 attactggtg atttttctct ccaatggcct gacgtggctg gtgcttttac tgtggggtga
    11761 gttgtttaaa cttgttggca tcacgttttt caatacgctc ttttttgcaa ccgactggtt
    11821 tatgtatctt acatttggtc tggttaccgc actggtggtg atcctcgcgc gtacacagtc
    11881 acgtttaatc gactctattc aaaagttgtt cacgctaatc gccacggggt tgctgccgtt
    11941 agtatcattg ctaaccctga tgtttatcat caccctgccg tttacggacc tgagcgcgat
    12001 ttctcgccac atctccgccg ccgggttgct gttaacgctg gcctttttgc aattgatctt
    12061 aatggctatt gtccgcgatc cgcaaaaagc gtcacttccc tggacggggc cgttgcgttg
    12121 cctgattaaa accgctttgc tggttgctcc gctgtatgtg ttcgtcgccg cctgggcgtt
    12181 atggctgcgg gtcgctcagt acggctggac tgtcgaccgc ttgcagggcg cgctggcggt
    12241 gctggtgtta ctggtgtggt cgctggggta ttttgtcagt atcgtctggc gtaatgggca
    12301 aaatcccctt gttcttcagg gcaaagtgaa ccttgcggtt tcgttattgg tgttggtgat
    12361 actggtgctt cttaattcgc cggtgctgga cagtatgcgc attagcgtga acagtcatat
    12421 ggcgcgttat cagagcggca aaaacacgcc agaccaggta actatctaca tgctcgaaca
    12481 gagcggtcgc tatggacgtg cggcgcttga gtcgctgaaa agcgatgccg ggtttatgaa
    12541 agacccgaaa cgcgcgcggg atctgctgat ggcgttagat ggagagcaac atcttcagga
    12601 acaggtatcg gaaaaagtat tagccgaaaa tgtgttaatt gctcctggtt ctgttaaacc
    12661 ggatgcgaca ttctggtcgg ccttaatcca ggatcgctat aacgtgatga cctgtattga
    12721 aaaagacgcc tgcgtcctgg tcgagcaaga tctgaatagt gatggtcagg cggagcggat
    12781 cctgtttgct tttaatgatg acagagtcat tgtctatggc tttgactcag acagaaaaga
    12841 atgggacgcg cttgatatga gtttacttcc gaacgaaata acgaaagaaa aattactcac
    12901 agctgcgaag gatggaaaac tggatctgac ggtagatggt gaaacgctgg aaataaactt
    12961 aagtaagtga caactctttt aacggatacg acgctgtatc tgaccaacaa taatttgcga
    13021 tttatttcac aggcctggta agcgcagcac atcaggccat tacgtcgtaa aatccggcag
    13081 agccctctct gccggacata ctcattaatg aacttgcgga tctgccggag acgcgttgtt
    13141 gcggatttca gcaatatcca tcgcattgaa cagatagtgg ttaccgcagt aatcacaatg
    13201 catgtcaatt tcgccatctt ccgccaggat gctatcgact tcttcatcag gcagcgtttt
    13261 cagcgcgtcg gcgcaacgtt cacgcgagca ggtgcatttg aactccacat cctgcggatc
    13321 gtaaaccgtc acctcttctt cgtgatacaa acgccacaac acttcgtttg ccggtaaggt
    13381 cagcagttct tcggttttga tggtttcggt tagcgtcgcc aggtggtcaa agtcgtcctg
    13441 ctgggcattt tgcgcaggca ttacctgcaa caacataccg cctgcagccg gtttgccgtc
    13501 tacgtcgccg gtgcgaataa acaggcgcgt cggcagctgt tcagaacgca taaagtaatc
    13561 ttccaggcag gccgccaggg tatcaccttc cagaccaacc acgccctgat agcgttcgcc
    13621 ttcgctcggg gtaatggtga tcaccacgta accattgccg accagcgttt tcaggtcggc
    13681 attttctgga atttcgccct gcacgcgcgc cacaccgcgc atttgctggt tattgttacc
    13741 gttaataacc gccagattca tcggaccgtc gccttgcagc tgtacggtga tatcaccatc
    13801 aaacttcagc gtagcggtta acaggctggt cgcaaccagc agttctgcca gcacgttttt
    13861 aacgggctgc ggataatcgt gattctcaag gatctgttgc agggtttccg aaacggttac
    13921 cagttcgccg cgcacggcaa agttttcaaa cagatagcga tgtaattggt catgttgcgg
    13981 cataatcatc tctcttgcag gtgacagtta ttcactgtcg ccgtgtttaa atcgtaacag
    14041 gtcgcggcgc tcttttttgt ccgggcgtcg gtccgggtgc ggcatggtta aggcattaag
    14101 tttacgtgcc agcgccattt tttcgcgttt ctctacactt tccgcagtct cttcatacag
    14161 caaggctgcc tcgctggcgg ggcgacgctg ttcagtaatc gcctttacaa tcaccgtgcg
    14221 ttcgtcattt ccctggcgca gagtgagcgt ggcattcagc tcgacgattt tgctcggctt
    14281 gctgcgctgc ccgttgtaat gcaccttacc gccttcaatc atttcacggg ccagcgcgcg
    14341 ggttttataa aaacgggcag cccatagcca tttatccagt cgaacctcaa cagcaggttt
    14401 ttctttcatg gcgtctcctt cacattagcg aggggatcag gcggcggtag tcattcagtg
    14461 acggatggcg ttgatactgt ttctcggcaa tcccggaatc aggattagtc acgccgaggc
    14521 agtaacgaat accaaattgc gcggcagcat cgagaatcgc ttcgctgtca tcaataaaca
    14581 gcgttctttc agctttcaga cccgtagctt cggccaccgc atgccataac cgctgatcct
    14641 ctttcggata accaaatgtg tgggtggaaa gtaataaatc aaggtgtgcg tccagaccgg
    14701 tatgctcaag ttttaccgcc aggttgtgcg gatgcgcatt ggtgagcaaa attcgctgct
    14761 taccgctggc tttcagtgcc tcaagaaacg gaatggtatc ttcacgcagt acggcacgcg
    14821 gtcccatctc ggtggtcatc gcacagatat ccagacccag ttgctcactc cagtaatcaa
    14881 gacagtacca gtttagcgta tgctgtacgt cgtgatattg ctggcgcata tattccatcg
    14941 cttcctgtgg cgtaaccccg tttttcgcgc cccatgtttc aggcaccagc ttttgccaga
    15001 aatagttatc gaaggcgagg tcgagcaacg tgccgtccat atccagcaga acggtatcta
    15061 cgtcctgcca ggcaatgttg atatgcatga gggaaatctc cagagtgaag caatttgcgc
    15121 gacagggtag cataacctgc cgcgcaaacg tgttattcga taaggctttc tgaaggggtg
    15181 atcagttgcg ggttcaggca gctttcataa taggcctgaa tcttcatcat gcgagtgcga
    15241 tgacgctggt aacgacgcca ggcctgtacg ccattgtaaa tggcgctacc cagcatcgtc
    15301 agtagcagca atgtggtgct gagatagcgc cacaggccgg aacgatccgg gattggatga
    15361 aggccaatat gctgcgtacc attggcgtca gtgaagattt ttgtgacgat accctcggcg
    15421 ttaaacggcg tatgcatcag catttgtgcc agtttctgga aagcgttcca ctgttcttgc
    15481 ggcgggtagt cgtaaagtga tgccgaaggc cagggctgat caacaaaatc gctgccttcg
    15541 tcgctgacaa tcaggaatcc gccgggggcc ggactgttta gtgattgtgc cgctcgcgcc
    15601 gtttcatgcg tgataaacgg cgcggtggag gttgccacca ggttatccag cgattccgca
    15661 ctcaccgggc gtaataacac attcacgcca tcgagcttcc cggcgttggc gcgttttacc
    15721 agcgcgtccc agtctttact gttgccgaga ttgaccagcg catttttcag tcgcacacag
    15781 tcatctttgg cagaacataa atccgctgtc ttcagtacaa tgtcgccaaa atcatcaagc
    15841 aataccatgc cggatttttg aattgctgaa cgtaatgagg cactgacgcg agatccatct
    15901 tccggtttag ggtgcagctg gcgattaact gcttcagtca atgccgtcgc tttgttgacc
    15961 agttcagatt ctggtaatgg caatgagcgg gcgtcgttcc agatgatctg cgagcagtca
    16021 aacggtaaaa aaggtgaatt ggttttcgcg ctccaggttc cggaagtgcg aatattacac
    16081 attcccgtac cgctgatacg caatgtatcg cctacccgca cgccagcgtc agccagttgt
    16141 tttacgctgg tggcttcaat ggtctgtgcg cctttcatcc atgagagagt gaatttcagc
    16201 ggcatatcca gcgggatcca gaataacagc ataaacagca ccagcagcga gcccgcagcg
    16261 ataatcgtac tgcgcagcca gtgctgtaac ggaaagtttt ttacttcatc atgcagcgaa
    16321 agatatcgcc cctgacgcac tacgtggcgg tcgagataga tatcaatatc ggtttgttga
    16381 ccgagatcct gagcaatgta tggctgccag tgtgcgggat agaccaggtc gataataccg
    16441 agtgaaatat tgttgatctg ttcctgatcg ttttcgccaa acaatcccca acgacggggt
    16501 gtaccgcgca gacaatgaat ttcccgcagg gaggattttg ccgggggcgc gaatagcccc
    16561 cacagacctg cgcccagcag cagtaatgcg ccgcctgcca gccacggaac aaatacatcc
    16621 ggggtaatca ggcagaaaaa gaacatcagg aaggaggcga cgatcagcaa cgcttcacgc
    16681 agcccgcgcg gacgactcag cgcatattct tcatgcgttt ctttgcgaat attgagtagc
    16741 tcgatttgct cactctcttc cccgcgaatc gacgcctgcg tggacggaac aggttgcaag
    16801 gcatagccgc gcgtttcctg catgtactcc tgcagcgtat gaccgttgag tgaaataacc
    16861 agcggcagcg aatcggtata aatcagttca acggtattct catcgttgat gtattgttcc
    16921 cagaaagggg gcaggtggac ttctaccgaa tcgaggtagt aacgccattt attcggatcg
    16981 tcggtagaga tgccgtaacg cgtgatagcg tgtgtcagca tcatgacgtt gttgctttcg
    17041 gcattcagcg ccagagagat cggtgccgcg ctagctcccg ttgggccagg cacctgtaat
    17101 acctgcgtca ggctctcaag ataattttca acggcgctac gttcttcagg tgtgagttta
    17161 cgcgtttgcg catccgcgaa ggcgttggtc cagggcagct gacgccgtct ggatcgcact
    17221 tttatcagcc atcccgcaag tagtgagcag gccagcaaag cagctaaaaa aatcacaatg
    17281 gtgctcatgc tttccccatc ttacttagtc tgtcaggcgt ggtcagtcgc accagacaca
    17341 atacatgatt ctgcggttgg ctatcggcaa gcgtggagtt taacttgagc atcattaagc
    17401 acatcacagt aaccgctgat catagcaaac cctctgaagg cgggatatca gcaaattcct
    17461 ggagttattt caacctattg acttttaatt tgatttgagg acgattattt attaagttac
    17521 ataaatgtaa ataacatgca attcacattg ccgaaagcgt gtccgatatc gcacaataac
    17581 cgctgatttc acagcataga tcccacgatg agcaaatcat tacaaaaacc caccattctg
    17641 aatgttgaaa ctgtagccca ttcccgactg tttaccgtcg agagcgtgga tctggagttc
    17701 agcaatggcg tgcggcgtgt ttatgaacga atgcgtccaa ccaaccggga agcagtgatg
    17761 attgtgccga ttgtggacga tcacctgatc ctgatccgcg aatacgcagt gggaactgaa
    17821 tcctacgaat taggtttttc gaaaggatta attgatccgg gtgaaagcgt ctacgaagcc
    17881 gctaaccgcg agctaaaaga agaggttgga tttggtgcga acgatctgac ttttttgaag
    17941 aagctcagca tggcaccgtc ttacttttcc agcaaaatga atatcgtggt cgcgcaagat
    18001 ctctacccgg aatcgctgga aggcgatgag ccagagccgc taccacaggt gcgctggccg
    18061 ctggcgcata tgatggattt gctggaagac cctgacttca acgaagcgcg taatgtcagc
    18121 gcgctgttcc tcgtgcgcga atggttgaaa gggcaggggc gagtgtaaat gcgtgaagtt
    18181 tgcctgatgc acaacgatta tcaggcttat ggggttcctg caatatattg atttttcacg
    18241 attttgttag ccggataacg cgttcacccg catccggcat aaacaaatgc actttgtcag
    18301 ccaactgaaa aggcgccgaa gcgccttttt aatcagaaca attcctgtgc ctcaccatta
    18361 tcgataatgg ttgttcccac ctcgtgcacc gcctgttgtg tcggctgcgt accttcgatg
    18421 aaatactctt cgcggctgtt gccaccatta gctaactgcc cggtgctgcg atcgatattc
    18481 accgtcacaa tacccggagg cggcgtcagc ggctgctccg gcacaccttc aagaacggct
    18541 ttcatataag cgtcccatgc aggctgggca ctcttggcac cgccttcgta acctgagatc
    18601 tgatctttaa tcgctccgga agccgttgta tgaccgagat tacgacggtg atcatcaaag
    18661 ccaatccaga ccgaggtcac aacgcccgga ccgtaacccg agaaccacgc atctttcgaa
    18721 ctgttagtgg taccggtttt cccgccgata tcgcgacgct gcaaatcacg acctgcacgc
    18781 cagccggtac cctgccagcc tggttcacca aagatattgg tgttcaaagc actcttaatc
    18841 aggaatgcca gcggagtgtt gatgacgtgc ggtgcgtact cctgcgcgcc agtcttcgcc
    18901 actaacgcct gatttgcctg ctccagctgc ggcattggta cagaaacatt ctgctgctcg
    18961 cgggagatag cgacatcttc aacatcgtta ttttccagca cgttcgattt ctgcgtatca
    19021 ccgtaaatca ccggaatatc gcattccggg caggctactt tcggtttcgc ttcgaaaatc
    19081 acgccgccct gatcgttttc aattttgctg ataaaccacg ggtccaccag gaagccgccg
    19141 ttcgccatga ccgcgtagcc gcgcgccacc tgcattgggg tgaaggacgc tgaacccagc
    19201 gccagcgatt cggtgtggac aatgttttgc gccgggaagc cgaagcgttg cagatattct
    19261 gcagcgtagt cgacgcccat cgcccgcatt gcacgtacca tcaccacgtt tttcgactga
    19321 cccagcccct gacgtaagcg aattggacca gcatactgcg gtggtgagtt cttcggctgc
    19381 cagtcagaac cgacacctgc atcccagcga gaaattggca catcgttcaa catacttgcc
    19441 agcgtcagac ctttatccat cgccgcggtg tagaggaacg gtttgatgtt ggaacccacc
    19501 tgacgcagtg cctgggtggc gcggttaaac ttgctctgat tgaaatcaaa gccaccgacc
    19561 agcgccataa cggcaccatt ttgcggattg atcgacacca gcgccgagtt cacttccggc
    19621 acttgtgcca gccaccatgc atcgccaacc tgacgaaccc agatttgctg acccgtttgc
    19681 agaacatcgg tcactttacg cggcgtcggt ccctgctgag tatccgaacg gtaaggacgc
    19741 gcccagcgaa cgccgtccat actcaatgcg acagtcgacc cgtccgccag cattgccgtc
    19801 gcttcctgag gattggcgct ggtgactgcg gcaggcagca gcggaccata ggtcggcagc
    19861 gccttcagcg tatcggtaat cttgttgtta tcccacgccg actcgcccac tttccacagc
    19921 acatttgccg ggccgcgata gccgtggcgc atgtcgtagt ccagcacgtt attacgtacc
    19981 gcctgctgtg cggcctgctg cactttgcgg gtgatggtgg tgtaaatgcg ataaccgtct
    20041 tcataggcac tttcgccata acggttatac atctcctggc gcaccatttc gctcaggtac
    20101 ggcgcagaga aagcaatctc cggcgcgtga tagttagcgt taatcgcctc agtgcgtgtc
    20161 tgatcgaact gttgttgggt gatataccct tcatccagca tccgcgacag tacgacgtta
    20221 cgccgcgcga cggcacgatc cattgagtag agcggattaa aggtggaagg cgctttcggc
    20281 agcccggcta tcacagccat ttcgttcagc gtcagttggt cgaccgtttt accgaaatag
    20341 acttgtgccg cagcaccgac accataggcg cggtaaccga ggtaaatctt gttcagataa
    20401 agctcgagga tctcgtcttt cgtcagcaac tgttcaatgc gaatcgcgag gaagacttcc
    20461 ttaatcttac gcatcagcgt gcgttctgga ctgaggaaga agtttctcgc cagctgctgg
    20521 gtaatggtac tcgccccttg tgacgcgtgg ccggagaaca gcgccacact tgccgcacgg
    20581 aagatcccca ccgggtcaac gccgtgatgc tcgtagaagc ggctgtcttc cgtcgcgata
    20641 aaggctttca ccatctccgg cgggatttga tccaacgtaa ccggaatacg acgtttctca
    20701 ccgtattgag caatcagctc gccatcggcg ctgtaaatct gcatcggaat ttgcaggcga
    20761 acatctttta atgtcgccac atccggcagt tgtggctcga tgtagcggta taggccataa
    20821 atcgagcctg ctcccagcag aatgcaacag actgcaagga tcaaaaaata ctttacgaac
    20881 ttcactggaa atttcccatt tagtttcatt tgggcagttt ataaacaaac gtgcggtagt
    20941 ataaaggcaa gccagacgca ttgatatacc cgtcagagtg acgggtgata aggagatcat
    21001 cacaatggca tttaagatct ggcaaattgg tttgcattta caacagcaag aagcagtagc
    21061 ggttgcgatc gtgcggggcg caaaagaatg ctttttgcaa cgctggtggc ggttgccgct
    21121 ggagaacgac attatcaaag atgggcggat tgttgatgcg cagcggctgg ctaaaacgtt
    21181 gttaccgtgg agtcgcgaac tgccgcagcg tcatcacatt atgttggcgt ttcccgccag
    21241 tcgcacatta cagcggtcat ttccgcgccc gtcgatgtcc ctcggtgagc gggagcaaac
    21301 ggcctggctg tcagggacga tggcccgcga gctggatatg gatccggact ccctgcgttt
    21361 cgattacagc gaagactcac tcagtcccgc ctataacgtg actgccgcgc aaagcaaaga
    21421 gctggcaacg ctactcactc tggcagaaag gttgcgtgtt catgtgagcg cgatcacccc
    21481 ggatgccagt gcattacagc gattcctgcc ttttttacct tctcatcagc aatgtctggc
    21541 ctggcgtgat aacgaacagt ggctgtgggc gacacgctgt cgctgggggc gcaaactggc
    21601 ggtggggatg actagcgcga aggagctggc ggcagcgtta tccgttgatc ccgaaagcgt
    21661 cgcgatatgt ggcgaaggcg gatttgatcc ctgggaggcc gtttctgttc gtcagccgcc
    21721 gctaccgccg ccaggtggag actttgccat cgcgctgggg ctggcgcttg ggaaggcgta
    21781 ctgatgaacc cgccaattaa ttttttgccc tggcgacagc aacgccggac cgcttttctt
    21841 cgtttctggt tgctgatgtt cgttgcgcct ctgctgctgg ccgtcgggat aacgctaata
    21901 ctgcgtctga cagacaacgc cgaagcacgc gtaaacaccg ttttgcttca ggcggaacaa
    21961 caactcgccc gcagcttaca gataacgaaa ccacgtttgc tggagcgcca acaaattcgt
    22021 gagcagcgtt tgcaacggca gcgccagcga caatttaccc gcgactggca atctgcgctg
    22081 gaagcactgg cggcgctttt acccgagcac gcctagctga caacgataag ctggcagcag
    22141 ggaacgctgg agatcaaggg gctgacaaca agcattaccg cgttaaacgc actagaaacg
    22201 tcactccgcc aggatgcgtc ttttcatctc aatcagcggg gagccacgca gcaggatgcg
    22261 cagggacgct ggcaatttga gtatcagtta acaaggaagg ttagcgatga acatgttctt
    22321 tgactggtgg ttcgccacat caccccgcct ccgccagttt tgctgggcat tctggttgct
    22381 gatgttagtt acgctcattt ttctgtcatc gacacaccat gaagagcgcg acgcattaat
    22441 tcgactacgg gcaagtcatc accagcagtg ggccacactg tatcgcctgg ttgacaccac
    22501 tcccttcagc gaagaaaaaa cgctgccctt ttcgccactg gattttcagt tgcccggcgc
    22561 gcaactggtt tcctggcatc catccgcgca gggaggcgag ttggcgttga aaacgctgtg
    22621 ggaagcagtg ccgtcggcat ttacacggct ggcagagcgc aacgtcagcg tgagccgttt
    22681 ttcattaagc gtggaaggtg atgatctttt gttcacgcta caactggaga cgccgcatga
    22741 gggttaaacg ctggttgttg gcaggtattg tattgtgcct tttaaccggt atgcgtgacc
    22801 cttttaaacc gccggaagat ctatgccgga ttagcgaact tagccagtgg cgctatcagg
    22861 ggatggtagg gcgaggcgag cgcatcatcg gtgtaataaa agacgggcaa aagaaatggc
    22921 gacgggtgca gcaaaacgat gtgctggaaa acggctggac aattttacag ctgacgccag
    22981 acgtactaac gctgggtacc gggacaaact gcgaaccgcc acaatggttg tggcaacggc
    23041 aaggagatac aaatgaagca atggatagcc gcactactgt tgatgctgat acccggcgta
    23101 caggcggcaa agccgcaaaa agtgacgctg atggtggatg acgttccggt agctcaggtg
    23161 ttgcaggcgc tggctgaaca ggagaagttg aacctggtcg tgtcgccaga cgtcagcggt
    23221 acggtgtcgt tacatctaac agatgttccc tggaagcagg cactacaaac tgtagtgaaa
    23281 agcgccggac tgataacgcg gcaggaaggc aacattctct cagtgcattc cattgcctgg
    23341 cagaataaca atatcgcccg ccaggaggcg gagcaggcgc gggcgcaggc aaatctgccg
    23401 ctggaaaatc gcagtataac cctgcaatac gccgacgcgg gagaactggc gaaagcgggg
    23461 gagaagctac tgagtgccaa agggagtatg accgtcgata aacgcaccaa tcgccttttg
    23521 ctacgagata acaaaacggc gttaagcgcg cttgaacagt gggtagcgca aatggatctg
    23581 ccggtcgggc aggttgagct gtcggcgcat attgtcacca ttaatgaaaa aagtttgcgt
    23641 gagttaggcg tgaaatggac gctggccgat gcgcaacacg ctggtggcgt tgggcaagtc
    23701 accacgcttg gtagcgacct ctccgtagcg acggcgacaa cgcatgtcgg ttttaacatt
    23761 gggcgcatca acggacgctt gctggatctt gagctttccg cgctcgaaca aaaacagcag
    23821 ctggatatta tcgccagtcc gcgtctgctg gcctcacatc ttcagcctgc cagcattaaa
    23881 caggggagcg aaattccata tcaggtttcc agcggggaaa gtggcgcgac gtcggtggaa
    23941 tttaaagagg ccgtcctggg gatggaagtt acgcccacag tgttacaaaa aggtcgcatc
    24001 cggctgaaat tacacatcag ccagaacgtt ccggggcagg tgctacagca ggccgatggc
    24061 gaagtgctgg cgattgataa gcaggagatc gaaacgcagg tcgaggtcaa aagcggagaa
    24121 acgttggcgc tgggcggcat ttttacccgt aaaaataaat cgggtcagga tagcgtaccg
    24181 ttgcttggcg acattccctg gttcgggcaa ttatttcgtc atgacggaaa agaagatgaa
    24241 cgacgcgagt tagtggtgtt tatcacgcca cgactggttt ccagtgagta aacagccgta
    24301 aaagcggtaa tgtttttacg ctgaacgtgt ttcatctatt tgacgcgcgc aggtatttag
    24361 catacaagga gtaccgattt gagagttggt gctcttcgct gcctgcgttc catgatgatg
    24421 atttatcatt caggcggcat tttgctgtct tttttacgct aatcttaccc ggtgatttat
    24481 cgccagagcg gtggtagcaa ggcagcgcgc ttgcagcgac cagatatgca gagggatggg
    24541 tgatttattc agttgccaaa cccgctggag tattgagata attttcagtc tgactctcgc
    24601 aatatcttat gaggtttcag ttcatgtcct gcggcgctct ctgagcgaag cgggtttatc
    24661 attaacgaat agtcttagta gtaccgaaaa aatggcagag aaacgcaata tctttctggt
    24721 tgggcctatg ggtgccggaa aaagcactat tgggcgccag ttagctcaac aactcaatat
    24781 ggaattttac gattccgatc aagagattga gaaacgaacc ggagctgatg tgggctgggt
    24841 tttcgattta gaaggcgaag aaggcttccg cgatcgcgaa gaaaaggtca tcaatgagtt
    24901 gaccgagaaa cagggtattg tgctggctac tggcggtggc tctgtgaaat cccgtgaaac
    24961 gcgtaaccgt ctttccgctc gtggcgttgt cgtttatctt gaaacgacca tcgaaaagca
    25021 acttgcacgc acgcagcgtg ataaaaaacg cccgttgctg cacgttgaaa caccgccacg
    25081 tgaagttctg gaagcgttgg ccaatgaacg caatccgctg tatgaagaga ttgccgacgt
    25141 gaccattcgt actgatgatc aaagcgctaa agtggttgca aaccagatta ttcacatgct
    25201 ggaaagcaac taattctggc tttatataca ctcgtctgcg ggtacagtaa ttaaggtgga
    25261 tgtcgcgtta tggagaggat tgttgttact ctcggggaac gtagttaccc aattaccatc
    25321 gcatctggtt tgtttaatga accagcttca ttcttaccgc tgaaatcggg cgagcaggtc
    25381 atgttggtca ccaacgaaac cctggctcct ctgtatctcg ataaggtccg cggcgtactt
    25441 gaacaggcgg gtgttaatgt cgatagcgtt atcctccctg acggcgagca gtataaaagc
    25501 ctggctgtac tcgataccgt ctttacggcg ttgttacaaa agccgcatgg tcgcgatact
    25561 acgctggtgg cgcttggcgg cggcgtagtg ggcgatctga ccggcttcgc ggcggcgagt
    25621 tatcagcgcg gtgttcgttt cattcaagtc ccgacgacgt tactgtcgca ggtcgattcc
    25681 tccgttggcg gcaaaactgc ggtcaaccat cccctcggta aaaacatgat tggcgcgttc
    25741 taccagcctg cttcagtggt ggtggatctc gactgtctga aaacgcttcc cccgcgtgag
    25801 ttagcgtcgg ggctggcaga agtcatcaaa tacggcatta ttcttgacgg tgcgtttttt
    25861 aactggctgg aagagaatct ggatgcgttg ttgcgtctgg acggtccggc aatggcgtac
    25921 tgtattcgcc gttgttgtga actgaaggca gaagttgtcg ccgccgacga gcgcgaaacc
    25981 gggttacgtg ctttactgaa tctgggacac acctttggtc atgccattga agctgaaatg
    26041 gggtatggca attggttaca tggtgaagcg gtcgctgcgg gtatggtgat ggcggcgcgg
    26101 acgtcggaac gtctcgggca gtttagttct gccgaaacgc agcgtattat aaccctgctc
    26161 aagcgggctg ggttaccggt caatgggccg cgcgaaatgt ccgcgcaggc gtatttaccg
    26221 catatgctgc gtgacaagaa agtccttgcg ggagagatac gcttaattct tccgttggca
    26281 attggtaaga gtgaagttcg cagcggcgtt tcgcacgagc ttgttcttaa cgccattgcc
    26341 gattgtcaat cagcgtaaca acaagaaagg tcaggccgct tatcaagcgg tctattagct
    26401 tcaggttaat tgcaacgtgg taagcattaa ccttttagtg gggtgttaaa tggatgaatt
    26461 caaaccagaa gacgagctga aacccgatcc cagcgatcgt cgtactggtc gttctcgtca
    26521 atcttctgaa cgttctgagc gtactgaacg tggcgaaccg cagatcaatt ttgatgatat
    26581 tgaacttgat gacactgacg atcgccgtcc gactcgtgcg caaaaagagc gcaatgagga
    26641 accggaaatc gaagaagaaa ttgacgaatc cgaagatgaa accgtggatg aagagcgcgt
    26701 agagcgtcgt ccgcgtaagc gcaaaaaagc agccagtaaa cccgcttctc gtcagtatat
    26761 gatgatgggc gtcggcattc tggttctact gctgttgatc atcggtatcg gttctgcgct
    26821 aaaagccccc tcgacctctt ccagcgatca aaccgcgtct ggcgagaaga gtattgatct
    26881 tgcaggcaat gcgaccgatc aggcgaatgg ggtgcagcca gcgccgggaa ccacgtctgc
    26941 ggaaaatact cagcaggatg tttctctgcc accgatctct tctacgccga ctcaagggca
    27001 aaccccggcg gcaacggatg gtcaacaacg tgttgaagtg cagggtgacc tgaacaatgc
    27061 gctgacccag ccacaaaatc agcaacagtt gaacaatgtg gcggtcaatt ccacattgcc
    27121 gaccgaaccc gcgactgtcg cgcctgttcg caatggcaat gcatcgcgtg acacggcgaa
    27181 aacgcaaacc gctgaacgtc cgtccactac gcgcccagct cgtcagcagg cggtgattga
    27241 accgaaaaaa ccgcaagcaa ccgtgaaaac ggagccgaag ccggtagcac agacgccgaa
    27301 gcgtactgaa ccagctgccc ctgtggcgag cacgaaggca ccgactgcga cttctacgcc
    27361 agcaccaaaa gagacggcga ctacggctcc agtacagacg gcatccccgg cgcaaaccac
    27421 ggcaacacca gccgctggag ggaagaccgc aggtaatgtt ggttcgttga aatcggcacc
    27481 gtccagccat tacactctgc agctgagcag ttcctctaac tacgacaacc tgaacggttg
    27541 ggcgaagaaa gagaatctga aaaactacgt tgtctatgaa acggcgcgta atggtcagcc
    27601 gtggtatgtc ctggtttctg gcgtgtacgc ttcgaaagaa gaggcgaaaa aagcggtatc
    27661 tacattgcca gcagatgttc aggccaaaaa cccgtgggcg aaaccgctgc gtcaggtaca
    27721 ggccgatctg aagtaatcaa ggttatctcc cgcaatggtt tatcgttgcg ggagctgcct
    27781 gaagcgctgg atgctgtcgg agctttctcc acagccggag aaggtgtaat tagttagtca
    27841 gcatgaagaa aaatcgcgct tttttgaagt gggcaggggg caagtatccc ctgcttgatg
    27901 atattaaacg gcatttgccc aagggcgaat gtctggttga gccttttgta ggtgccgggt
    27961 cggtgtttct caacaccgac ttttctcgtt atatccttgc cgatatcaat agcgacctga
    28021 tcagtctcta taacattgtg aagatgcgta ctgatgagta cgtacaggcc gcacgcgagc
    28081 tgtttgttcc cgaaacaaat tgcgccgagg tttactatca gttccgcgaa gagttcaaca
    28141 aaagccagga tccgttccgt cgggcggtac tgtttttata tttgaaccgc tacggttaca
    28201 acggcctgtg tcgttacaat ctgcgcggtg agtttaacgt gccgtttggc cgctacaaaa
    28261 aaacctattt cccggaagca gagttgtatc acttcgctga aaaagcgcag aatgcctttt
    28321 tctattgtga gtcttacgcc gatagcatgg cgcgcgcaga tgatgcatcc gtcgtctatt
    28381 gcgatccgcc ttatgcaccg ctgtctgcga ccgccaactt tacggcgtat cacacaaaca
    28441 gttttacgct tgaacaacaa gcgcatctgg cggagatcgc cgaaggtctg gttgagcgcc
    28501 atattccagt gctgatctcc aatcacgata cgatgttaac gcgtgagtgg tatcagcgcg
    28561 caaaattgca tgtcgtcaaa gttcgacgca gtataagcag caacggcggc acacgtaaaa
    28621 aggtggacga actgctggct ttgtacaaac caggagtcgt ttcacccgcg aaaaaataat
    28681 tctcaaggag aagcggatga aacagtattt gattgccccc tcaattctgt cggctgattt
    28741 tgcccgcctg ggtgaagata ccgcaaaagc cctggcagct ggcgctgatg tcgtgcattt
    28801 tgacgtcatg gataaccact atgttcccaa tctgacgatt gggccaatgg tgctgaaatc
    28861 cttgcgtaac tatggcatta ccgcccctat cgacgtacat ctgatggtga aacccgtcga
    28921 tcgcattgtg cctgatttcg ctgccgctgg tgccagcatc attacctttc atccagaagc
    28981 ctccgagcat gttgaccgca cgctgcaact gattaaagaa aatggctgta aagcgggtct
    29041 ggtatttaac ccggcgacac ctctgagcta tctggattac gtgatggata agctggatgt
    29101 gatcctgctg atgtccgtca atcctggttt cggcggtcag tctttcattc ctcaaacact
    29161 ggataaactg cgcgaagtac gtcgccgtat cgacgagtct ggctttgaca ttcgtctgga
    29221 agtggacggt ggcgtgaagg tgaacaacat tggcgaaatc gctgcggcgg gcgcggatat
    29281 gttcgtcgcc ggttcggcaa tcttcgacca gccagactac aaaaaagtca ttgatgaaat
    29341 gcgcagtgaa ctggcaaagg taagtcatga ataagtttga agatattcgc ggcgtcgctt
    29401 ttgatcttga tggtacgctg gtcgacagtg ctcccggtct tgctgctgcg gtagatatgg
    29461 cgctgtatgc gctggagttg cccatcgcag gtgaagaacg cgttattacc tggattggta
    29521 acggcgcaga tgttctgatg gagcgcgcat tgacctgggc gcgtcaggaa cgtgcgactc
    29581 agcgtaaaac aatgggtaaa ccgcccgttg atgacgacat tccggcagaa gaacaggtac
    29641 gtattctgcg taaactgttc gatcgctact atggcgaggt tgccgaagag gggacgtttt
    29701 tgttcccgca cgttgccgat acgttgggcg cgttgcaggc taaaggcctg ccgctaggcc
    29761 tggtcaccaa caaaccgacg ccgttcgtcg caccgctgct cgaagcctta gatattgcca
    29821 aatactttag cgtggttatc ggcggcgatg atgtgcaaaa caaaaaaccg catccggacc
    29881 cgctgttact ggtggctgag cggatgggaa ttgccccaca acagatgctg tttgtcggcg
    29941 actcacgcaa tgatattcag gcggcaaaag cggcaggttg cccatcagtt ggcttaacct
    30001 acggatataa ctacggcgag gctatcgatc tcagccagcc tgatgtaatt tatcagtcta
    30061 taaatgacct tctgcccgca ttagggcttc cgcatagcga aaatcaggaa tcgaaaaatg
    30121 actaagccca tcgtttttag tggcgcacag ccctcaggtg aattgaccat tggtaactac
    30181 atgggtgcgc tgcgtcagtg ggtaaacatg caggatgact accattgcat ttactgtatc
    30241 gttgaccaac acgcgatcac cgtgcgccag gatgcacaga agctgcgtaa agcgacgctg
    30301 gatacgctgg ccttgtatct ggcttgtggt atcgatcctg agaaaagcac catttttgtt
    30361 cagtcccacg taccagaaca tgcgcagtta ggctgggcac tgaactgtta tacctacttc
    30421 ggcgaactga gtcgcatgac gcagtttaaa gataaatctg cgcgttatgc cgagaacatc
    30481 aacgctggtc tgtttgacta tccggtgctg atggctgcgg acatcctgct gtatcaaact
    30541 aatctggtac cggtgggtga agaccagaaa cagcacctgg aactgagtcg cgatatcgcc
    30601 cagcgtttca acgcgctgta tggcgagatc tttaaggtgc cggagccgtt tattccgaaa
    30661 tctggcgcgc gcgtaatgtc gctgctggag ccgaccaaga agatgtccaa gtctgacgat
    30721 aaccgcaata acgttatcgg cctgctggaa gatccgaaat cggtagtgaa gaaaatcaaa
    30781 cgcgcggtca ctgactccga cgagccgccg gtagttcgct acgatgtgca gaacaaagcg
    30841 ggcgtttcca acctgctgga tatcctttct gcggtaacgg gccagagcat cccagaactg
    30901 gaaaaacagt tcgaaggcaa gatgtatggt catctgaaag gtgaagtggc tgatgccgtt
    30961 tccggtatgc tgactgaatt gcaggaacgc tatcaccgtt tccgcaacga tgaagccttc
    31021 ctgcaacagg tgatgaaaga cggcgcagaa aaagccagtg tacacgcttc ccgtacgctg
    31081 aaagcggtgt acgaagcgat tggttttgtg gcgaagccgt aatttaacat taatgccaaa
    31141 aaccggggaa acccggtttt tttatcctca tttgcaataa tccgaaaaaa tgtgaagcgc
    31201 ctcgccgttt ccacatctcg tcgttgccac ttatcttcat tttaatgaag atataaatat
    31261 tcattttatt gaaaatatat tatgcgtcga acgtttatca aaaaagaagg cgtcgtcatc
    31321 acgacgctgg cccgttattt gttgggtgaa aaatgcggta atcgactgaa aaccatagat
    31381 gagctggcaa ctgaatgccg ttcatcggtt ggcctgacgc aggccgcgtt gaaaacgttg
    31441 gaatcaagcg gagcgatacg gattgaacgc cgtgggcgca atggcagtta tctggtcgag
    31501 atggataaca aagcattgct gagtcatgtg gatatcaaca acgtggtatg tgcgatgcct
    31561 ttgccctata cccgcttgta cgaaggttta gcgagcgggc tgaaagctca gtttgatggc
    31621 attccttttt actatgcgca tatgcgcggc gcggatattc gcgtggagtg tttacttaac
    31681 ggcgtgtatg acatggcggt ggtttcccga ctggcggcgg aaagttatct cacgcaaaaa
    31741 gggttatgcc tcgcgctgga gttggggccg cacacctacg ttggcgagca ccagttgatt
    31801 tgccgtaaag gcgagtccgc aaacgtgaag cgcgtggggc tggataaccg ttcggcggat
    31861 cagaaaatca tgaccgatgt cttttttggc gatagtgatg tggaacgtgt cgatctctct
    31921 tatcacgaga gtttacaacg cattgttaaa ggcgatgtcg atgcggttat ctggaacgtg
    31981 gtggcggaaa acgaactgac catgctggga ttagaggcga cgccgctcac agacgatccg
    32041 cgatttttac aggccaccga agccgtggtc ctgacgcgag tcgatgatta cccaatgcaa
    32101 caactgctgc gtgccgttgt agataaacac gccctgctcg cccatcaaca acgggtagtg
    32161 agtggggaac aggaaccgag ttattaaacg aaaggcatct gatatggaaa ccagactcaa
    32221 cctgctttgc gaggcaggcg ttattgataa ggacatctgc aaaggcatga tgcaggtcgt
    32281 caacgtactg gaaacagagt gccatctgcc ggtgcgcagt gagcaaggaa cgatggcgat
    32341 gacacatatg gcgagtgcac tgatgcgcag tcgccgtggt gaagaaatag agccgctgga
    32401 taacgaattg ctggcagagc tggcgcaatc cagccactgg caagccgttg tgcaattgca
    32461 tcaggtgttg ttgaaggaat tcgcactgga agttaacccg tgtgaagaag gctatttgct
    32521 ggccaacctt tatggattat ggatggctgc taacgaagag gtttgaagct cccgacaaat
    32581 ccacgtcgca caacctaaat attcaaaaaa atgaatattt aattcaagga acagaaacga
    32641 ggcaacaaga tgtttgtaga agcattgaaa cgccagaacc cggcgctgat ttccgccgca
    32701 ctaagtttgt ggcagcaggg caagatcgcc ccggacagct gggtgatcga cgtggatcag
    32761 gtactggaaa acggtaagcg actgattgag acggcgcggc tttacggcat tgaactgtat
    32821 ctgatgacca agcagtttgg ccgtaaccca tggctggcgg aaaaattgct ggcattaggt
    32881 tacagcggca ttgtggcggt ggattacaaa gaggcgcgag tcatgcgccg cgctggtttg
    32941 tcggtggcac atcaggggca tctggtacaa atcccttgtc atcaggttgc tgacgccgtt
    33001 gaacagggca ccgacgtcat caccgtgttt actctcgaca aagcgcggga agtttctgcg
    33061 gcagcggtga aggccggacg tgtgcagttt gtgctgctca aagtttatag cgacgatgat
    33121 tttctttatc cggggcagga gagcggtttt gtacagcatt cgctgcacga ggtggttgct
    33181 gaaattaaga aactgccggg gctgcattta gccggactta cccatttccc ttgcctgctt
    33241 tgggatgagg ctgccggaaa agttttgccg acaccgaatc ttcacacgct gatacaggca
    33301 cgggatcaac tggcgaaatc tggtattgca cttgagcaac tgaacgcgcc ttcagcgacc
    33361 agctgcactt cgctgccatt actggcgtaa tacggtgtga ctcatgccga acccggtcat
    33421 gcgctgacgg gcactattcc ggcaaaccag cagggcgatc agtctgaacg tatcgcgatg
    33481 ctctggttga gtgaaatctc ccatcatttc cgtggcgaca gctactgcta cggcggcggt
    33541 tactaccgtc gtggtcatgc gcaacatgcg ttggtgttta cgccagaaaa tcaaaagatt
    33601 actgaaacca atctcaaaac tgtggatgac agcagtatcg actacaccct gtcgctggca
    33661 ggcgagtttc cggtaagcag tgcagtggtg ctctgtttcc gcacgcagat ttttgtcacc
    33721 cgtagcgatg tggtgctggt gtccggtatt caccgtggcg aaccgaaaat cgttggtcgt
    33781 tatgacagtc ttggaaactc tctgggggcg taatggcgcg atttgtggtg ttagtgattg
    33841 atagctttgg cgtaggggca atgaaagatg tcacgctggt gcgtccgcaa gatgcgggag
    33901 cgaatacatg tggtcacatc ctgagccagt tgccgcattt gcagctacca acgctggaga
    33961 agctggggct aatcaacgca ttgggttatg cgccaggcga tatgcagccg tcagattccg
    34021 caacctgggg cgtggcagag ctgcaacatg aaggtggcga tacctttatg gggcatcagg
    34081 aaattttagg cacgcgcccg ttaccgccgc tgcggatgcc ttttcgcgat gtgattgacc
    34141 gtgttgagca ggcattagta tccgccggtt ggcaggtgga gcgccgtggc gatgaactgt
    34201 aatttctgtg ggtcaatcag gcggttgcga ttggcgataa tctcgaggcg gatttaggcc
    34261 aagtctataa cattaccgcc aatctctctg tgatctcttt tgacgacgca atcaaaattg
    34321 gtcgtatcgt gcgtgagcag gtacaggtcg gtcgggtcat tacatttggt ggcctgttaa
    34381 ccgacagtca acgcattctc gatgccgcag aaagcaaaga agggcgcttt attggtatca
    34441 atgcgccgcg ttctggcgct tatgacaacg gcttccaggt cgtgcatatg ggctacggtg
    34501 tcgataaaaa agtgcaggtg ccgcaaaaac tgtatgaggc gggcgtgcca accgtgctgg
    34561 tgggtaaggt ggcagatatc gtcagcaatc cttatggcgt gagctggcaa aacctggtgg
    34621 atagccagcg gattatggat atcaccctcg acgaatttaa cacccatccg acggcgttta
    34681 tttgcaccaa tatccaggaa accgacctcg ctggtcatgc agaagacgtc gcacgttatg
    34741 ccgaacgttt gcaggtcgtt gaccgtaacc ttgcccggct tgttgatgcg atgcagccag
    34801 atgattgcct ggtcgtgatg gcggatcacg gcaacgatcc gaccattggt cacagccacc
    34861 atacccgcga agtggtgccg ctgctggttt atcagcaagg cctggctgct acgcagctcg
    34921 gtgtgcgcac cacgctttct gatgtgggag ctaccgtgtg tgaatttttc cgtgcgccac
    34981 cgccacaaaa tggtcgctct tttctttcct ccctccggtt tgcaggagac accctatgag
    35041 ttttgatccg acgggttaca ccctggccca tgagcatctg catattgatc tctccggctt
    35101 taaaaacaac gtggactgcc gccttgatca gtatgcgttc atttgccagg agatgaacga
    35161 cctgatgacc cggggtgtac gtaatgtgat tgagatgacc aaccgttaca tggggcgcaa
    35221 tgcgcaattt atgctcggtg taatgcgcga aaccgggatc aacgtggtgg cctgtactgg
    35281 ttattaccag gatgcgtttt tcccggaaca tgtggtgacc cgcagcgtgc aggaactggc
    35341 gcaggagatg gtcgatgaaa ttgaacaggg tatcgatggc acggagctga aagccgggat
    35401 catcgcggag atcggctcca gcgaaggaaa gattacgccg ctggaagaga aggtatttat
    35461 tgccgctgcg ctggcgcata accagaccgg acgcccgatc tccacgcata cgtcgttcag
    35521 caccatgggg ctggaacaac tggctctgtt gcaagcccac ggagttgatc tttcgcgcgt
    35581 caccgttggt cactgcgatc tgaaagacaa cctcgacaac attttgaaga tgatcgatct
    35641 cggcgcgtac gtgcagttcg acaccattgg taagaacagt tactacccgg acgaaaagcg
    35701 cattgcgatg ctccacgcgc tacgtgaccg tgggttgctg aaccgcgtca tgctgtcgat
    35761 ggatattacg cgccgctccc atttaaaagc caatggtggt tatggctatg actatttatt
    35821 gaccaccttt attccgcaat tgcgccagtc aggattcagt caggccgatg tggatgtgat
    35881 gttacgtgaa actccctctc aatttttcca ataaggacag actcatgaaa aagattggcg
    35941 ttgcaggctt acagcgtgag cagattaaaa aaaccattga agcgacggct actggcagtt
    36001 ttgaagtttt cattcacaac gacatggaag cggcaatgaa agtgaaatcc gggcaactgg
    36061 attactacat cggtgcgtgt aataccggtg cgggcgcggc attgtcgatt gccatcgcgg
    36121 tgattggcta taacaaaagt tgcaccattg ccaaaccagg cattaaagcg aaagacgagc
    36181 atatcgccaa aatgatcgct gaaggaaaag tggcgtttgg cctttccgtt gagcacgtcg
    36241 aacacgcgat tccgatgctg attaaccatc tgaaataaaa ggcacgacta tggatctgta
    36301 tattcagatt atcgtggtgg cgtgcctgac gggtatgaca tcgcttctgg cgcatcgctc
    36361 ggcggctgtt tttcatgacg gtatccgccc gatcctgccg caactgattg aaggctatat
    36421 gaaccgtcgc gaggcgggga gtatcgcttt tggtctgagc attggttttg tggcctcggt
    36481 ggggatctct tttaccctga aaaccgggct gctcaacgca tggttactct ttcttcctac
    36541 cgatatcctc ggcgtactgg cgataaacag cctgatggcg tttggtcttg gcgctatctg
    36601 gggcgtgttg atccttactt gcctgttgcc agtaaaccag ctgctgaccg cgctgccggt
    36661 ggatgtatta ggtagcctcg gggaattaag ctcgccggtg gtttctgctt ttgcactgtt
    36721 cccgctggtg gcgattttct accagtttgg ctggaagcaa agtctggtcg ccgccgtggt
    36781 tgttctgatg acccgtgtgg tagtcatgcg ctatttccca catcttaacc ctgaatccat
    36841 cgaaatcttt attggcatgg tgatgctgct ggggatcgcg ataactcacg acctgcgtca
    36901 tcgtgatgaa aatgatattg atgccagcgg gctttcggtg tttgaagaac gcacgtcgcg
    36961 gattatcaaa aacttaccct atatcgccat cgtgggagca ttgattgccg ccgttgccag
    37021 catgaagatt ttcgccggca gtgaagtgtc gatcttcact ctggagaaag cgtactccgc
    37081 aggcgtaacg ccggaacaat cgcaaacgct gatcaaccaa gcggcactgg cagaatttat
    37141 gcgcgggctg ggttttgtac cgttgattgc caccaccgcc ttagcaacgg gtgtgtatgc
    37201 agttgcgggc tttacctttg tttatgcggt gggctatctc tcgccgaatc cgatagttgc
    37261 agcggtatta ggcgcagtgg ttatttcggc ggaagtcttg ctgcttcgtt cgatcggcaa
    37321 atggctggga cgctacccgt cggtgcgtaa tgcgtcggat aacatccgta acgccatgaa
    37381 tatgctgatg gaagtggcgc tgctggtcgg ttcgattttc gcagcaatta agatggcggg
    37441 ttataccgga ttctctatcg cggttgccat ttacttcctc aacgaatccc tgggccgtcc
    37501 ggtacagaaa atggcggcac cggtcgtggc agtaatgatc accggtattc tgctgaatgt
    37561 tctttactgg cttggcctgt tcgttccggc ttaaggaagg cacctatgaa gacgtttcct
    37621 ctgcaaagtc tgacgattat tgaggcgcag caaaagcagt ttgcgctggt ggatagcatt
    37681 tgtcgccatt tccccggcag cgagtttctt actggcggtg atttaggctt aacgccagga
    37741 ctgaatcaac cgcgcgttac ccagcgtgtg gaacaggtgc tggctgatgc atttcacgca
    37801 caggctgcgg cgctggtgca gggtgcgggt actggcgcga ttcgtgcggc gttggcggct
    37861 ttgctcaaac cggggcagcg tcttctggta catgatgcgc ctgtttaccc gacgacacag
    37921 gtcattattg agcagatggg gctgacgctt attactgctg atttcaatga cctgtcggca
    37981 ctgaagcagg tcgtcgacga gcaactaccg gatgcggcgc tggtgcagca tacgcgccag
    38041 cagccgcagg acggctacat tctggcagat gtgctggcaa cattgcgctc ggctggcgtt
    38101 cctgcattaa ccgatgacaa ctatgcggtg atgaaagtgg cgcgaattgg ctgcgaatgc
    38161 ggggcaaatg tctcgacttt ttcctgcttc aagctgtttg ggccggaggg tgttggcgcg
    38221 gtggtaggcg atgccgatgt tattagtcgg attcgctcca cgctctattc cggcggcagc
    38281 cagattcagg gcgctcaggc gctggaagtc ttgtgtgggc tggtgtttgc gccagtgatg
    38341 cacgcggtgc aggcgggggt atctgaacgg ttgctggctt tgcttaacgg tggtgcggtg
    38401 ccggaagtga aaagcgcggt gattgctaat gcgcagtcga aggtgttgat tgtcgagttt
    38461 catcagccga ttgccgccag agtgctggaa gaggcgcaaa agcgcggtgc cttgccttac
    38521 ccggtgggtg cagagtcgaa atatgaaatc ccgccgctct tttatcgcct ttccggaacg
    38581 tttcgccagg cgaatccaca atcagaacat tgtgcgattc gcattaaccc gaatcgcaac
    38641 ggtgaagaga cggtgctgcg gattttgcgt gagagtattg ccagtattta atgctggata
    38701 gcaccgtcaa gctaaattcc gtactgaacg gtcccctcgc ccctttgggg agagggttag
    38761 ggtgagggga acgtacggga tgtaaaattc aacgcgccag catcgccgga tgcgatgctg
    38821 gcgcatctta tccggcctac ggttaatgtc ttttattatc aatcgttaag gtgatgcgat
    38881 tggcgcggca gaagagttcg gagacgtgca ccggtttgtc attctgatca aaggcgattt
    38941 tgctgatgcg aaacaacggt tcgcccagtt cgcatttcag ccattttgcc tgcgggcggg
    39001 tggcggcaaa gatatcgatg gtctttttgt cgctgaccac tcgcgtgtca aaacgctcct
    39061 gaaataactg ataggtggag cttccttcga cgtaaatctc atcaaagtca ggataacgcg
    39121 acagcgggat ccatgaacta tcaataaaca gcggctcttt atcgagatac atcacccggc
    39181 agagatggaa cacttcgctg tttccgggga tgttcagctt ttcgcaaaac ggcgcggcgc
    39241 tgacccgttc ctgttcgatc actttctctt tcgtcgcctt gccttgtgag acgccaaaat
    39301 cggtaaaacc actgacagta agcagggcgt tttcaacttt ctggctttgt acaaaggtgc
    39361 ctttgccctg ccagcggatc agtacgccgt ctgccactaa gtcgctgata gctttgcgaa
    39421 tagtaatgcg gctgacgtta tattgcgtac aaagctcgtt ttcggtaggg atctgttgcc
    39481 cggcctggta aaccccctgc gcgatatcat ccagcagccg ctggcggacg gtagcgtaga
    39541 ggagttgatg agagtagcgg tccgtagctg acataatcga ccctgaaata aagaatgaat
    39601 tacacggatt atagggtcgt tttccggcaa gtaacagcac gttggcgcgg agatgctcac
    39661 gccaacgtta tacctaccag gcaccgtgat actgaatggt tttcgccgcg cacgccgttc
    39721 cctgcgccat cgcctgcggt aatgtcatcc ccgcagacca gccgcaaagg aatccggcaa
    39781 tgaacgaatc tccggcaccc atggtgtcga taaccgtcac cggttcagga gcctgacgcc
    39841 agaactgcgc gccatcccag gcaatgctgc cgttttcacc cagcgtgaca atcactgttc
    39901 ctgcgccacg ggcaacaatc gctttcatct tcagacgcag cgcttcgtct tcttgcggtg
    39961 cggaggcaaa ggcaaaatcg agatgcggca ccagtgtctg ccagagcggg ctgtcccact
    40021 tgtcggagaa gtcgaaagcg gtaagtttgc ccgcagcgtg cagctgtggg aatgcgtctt
    40081 ccgcatgtcc ccagattgcc gcgtgcacaa tgtcatactg cgccagccag gcgtaatcct
    40141 cttcactcag ggcaaagtcg gccatcacgc cttcggtgta gtcgccaaaa acgcgatcat
    40201 tgtcgtgcag ttccacctga gtttgtgcgg taacgccgtg tttcgtatgg acatggctga
    40261 tatcgacgcc catgcgggcg agatcctgct tcagctttgt gccgtagtca tcgtcaccca
    40321 ccaaggtaat gcatcccggc tgtatgccgt agcgagtgca gtacaccgcc acattgaccg
    40381 cattaccgcc agaaaacgct ttattcagtt gcgggtagat atcgacgcag ttatcgccga
    40441 ttgtcgccag ggttttcatc tctcatcctc cggcagcagc gcgcgaaagc gttccagcgc
    40501 ctggcgggca tagagtctgg gctcgttcat atacatcgtc accagctcca ccgtacagta
    40561 accctcatag ccccgctcaa taatatcgcg catcagttcc cgcagcggca ttttgccttc
    40621 tccaggaatg taatgcgtgt cgctggcccc gtcgctgtcg acaatatgca gatgacgata
    40681 atttatcgcc cagtttgtcg aaataactca tcaccggttc cgcctggaca tacggcgcgc
    40741 aaatgtcgac catgctgaac aggcgcggcg aaggcaccag cgccagcgca tgaagcacat
    40801 cattagcatt acacacgacg ttcgattcat acggcgttaa cggttcgagg attagatcca
    40861 tgccgatgtt ttccgcgtac tcacacagtt cgcttaggtt ctctgccagc cgtccccaga
    40921 taacattagg tggcgtgaga tagcctgcgt gggccgcgga aatcagcgta taacccgcgt
    40981 tcatctcttt tgccatatcc atcgccagct tgatcatgtc gaggctttcg cgacgcatat
    41041 gttcatcgcc cagcatcatg ttatacggat agccgttggt ttctggcgta tagccgataa
    41101 tcggcatctg atacgtctgc gccagcgcct tgatttgttt gatgccgccc gcttttaagt
    41161 ccggcgcgaa cgcgtgcggg cgaccgcccc aaatttcgat gccgtcgtaa cccagctcgc
    41221 ttgcatcacg aaatgcgtgt tcaataggca gccgctggtg gccgcaggta aacatacctg
    41281 ttttcatcgg gtaactcctt gcgtaaatcc ggcccgttgc cgggccggga gagggattaa
    41341 tattccacca gaccaccgta atagcggcgt tcatccgggt tgtgatcttt gtaaatagac
    41401 aggtagtagc agagccactc cattggcacg aacatcagga acggtgccag ccacgggtgc
    41461 agcccttgcg aaatttcggc gtaatcgatg acgatcacgt tgtcagtacg ctgtttaaca
    41521 aagttaatgg cgcgttcggt ggtgtggcga ctttcatcat tgccgagcag gaacaggaac
    41581 ggaacgcccg gttcgacaat ctccagcggg ccatggcgga actctccgct ctcaatcacg
    41641 cagccgtgcg tccaggtaaa ttccatcagc gttacaatgc cttctttgta acccagcgga
    41701 cgcagcggac ccgcagcaac ggtataaatc atcggccact ggctggccag ttcaccaagc
    41761 tggcggcctt tttcttccca ggtgcgtacc agatgaccga gcgcattcgg caacggcttg
    41821 agatcgtttt tgatcttgcc gatttccgcg ttcggcgcga ggcgggtgat catctccagc
    41881 accacgctgt agcagagcag caggtgaatt tcccagatac agtcggcctg ataatcaatg
    41941 ctaaattccg ccgccgaggt aatcgggctg tccgcgcgtt tggtgaacgc cgcagtgagt
    42001 gcgccgcagg cccggcccag ctccagcgct ttgattacct cttccgtttt accgtagtca
    42061 gaaacgccaa tcaccgcgca acgatcgtcg aggcgatacg gggtgttatc gcagaactcc
    42121 cagccggaaa tggcgtagac ctgaagatcg gaaaagcgat ccgccagatg tttcgccgtt
    42181 tgcgccgcgt tgagtggcga gccgcaggca acgaaataaa tacgatcaat gtcgcgcttc
    42241 accatctctt ccacgatggc gtgcaccagc ggaacgtcat ggctgagaac tttttccact
    42301 tcctgaacca tattttcggt gaccagaaag tccacggtgc ttttatcaat atccaacatt
    42361 ttattttctc cagataacat tacgacaaag cgttgagctg gcggctacgc ttcgcccaga
    42421 aggcgtaagc aggcagacca gtagcaataa cgatgacggc gcagataagg ccgggaatcg
    42481 gtgcccagac aaaggttgag gcgaccagaa tgaggcttga cgcaatggcg agggtggtca
    42541 tcagcccgaa agccggagta cgccacagcg gtttgtaatc gtcgcgttta cgacaccaga
    42601 tgatggagcc gaaggtgagg gtatttttga aacacatcac cagggtgaaa taacccagca
    42661 ggctggtgag atcggaaacg aagatgaaga agatccccag cgccccttgc aggatgatgg
    42721 agacatccgg cgtgttgtat ttcggatgca catggccgaa gcatttaaag aacaggttgt
    42781 ctttcgccat cgcgtattcc agccgcggct ggtacatcac gcagctggaa agcgaaccaa
    42841 gaatgacgat catcgccgtg atggcaacaa agatcccagc ggtgctgccg agtgcgggga
    42901 tccaggtcag ggcgtcggaa atcggcgttt cagaattggc gagtttgtcg aagggcatca
    42961 ggccggaaat caccagcgcc agcagggtgt agagcaccag aaccagcaga caggaaccaa
    43021 tcagcgctcg tggcatggtt tttccggggt ttttaatttc gccggtcata taacagatag
    43081 aggccatgcc ggtatacgac caactggtgg cagagatccc cgccagcagc gccataaagc
    43141 tgcccgttgc gccaatcgca gtggtggtag gggcggcaaa attctccgct ttaaaccaga
    43201 agatcccaag gccaatgacg atagtgaacg ggataatttt ggcgatggta attagcgtct
    43261 gaaacgctgc gccgccttca acggagcgca ggtgtagcaa cataaaggcg ataattaatc
    43321 cggcggcgat aaatttaccg agcaacggat cgataggcgt taaaaagcca agattgctga
    43381 caatcgccag cgccataatc gacaatgacg gcgcatcgtt ggcccagaag ctggcccagc
    43441 cggagaggaa agccagcggt cggcttccgg catttttcag ataaacataa tctgcgccat
    43501 tttccggata agcggtggat agttccgcat agacgcacat ttgcgggatc acaattaacc
    43561 cgccaatgac aaacgcgagc accgtaagcc acggcgtgcc cgctgctttt gccacttcac
    43621 ccacagatac aaaaataccg gagccgacgg ttgtcccgac ggcgattgca agaacggccc
    43681 aaaatccgag cttgcgttgg agttcctggc ttccc
//
LOCUS       DAJTVK010000020        41013 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000020 DAJTVK010000000
VERSION     DAJTVK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.020"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            218..1882
                     /gene="asnB"
                     /locus_tag="OO372_001170"
     CDS             218..1882
                     /gene="asnB"
                     /locus_tag="OO372_001170"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6340732.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2262..2338
                     /locus_tag="OO372_001171"
     tRNA            2262..2338
                     /locus_tag="OO372_001171"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2296..2298,aa:Met,seq:cat)
     gene            2347..2431
                     /locus_tag="OO372_001172"
     tRNA            2347..2431
                     /locus_tag="OO372_001172"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2381..2383,aa:Leu,seq:tag)
     gene            2455..2529
                     /locus_tag="OO372_001173"
     tRNA            2455..2529
                     /locus_tag="OO372_001173"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2487..2489,aa:Gln,seq:ttg)
     gene            2564..2638
                     /locus_tag="OO372_001174"
     tRNA            2564..2638
                     /locus_tag="OO372_001174"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2596..2598,aa:Gln,seq:ttg)
     gene            2654..2730
                     /locus_tag="OO372_001175"
     tRNA            2654..2730
                     /locus_tag="OO372_001175"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2688..2690,aa:Met,seq:cat)
     gene            2779..2853
                     /locus_tag="OO372_001176"
     tRNA            2779..2853
                     /locus_tag="OO372_001176"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2811..2813,aa:Gln,seq:ctg)
     gene            2890..2964
                     /locus_tag="OO372_001177"
     tRNA            2890..2964
                     /locus_tag="OO372_001177"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2922..2924,aa:Gln,seq:ctg)
     gene            complement(3118..4293)
                     /gene="ubiF"
                     /locus_tag="OO372_001178"
     CDS             complement(3118..4293)
                     /gene="ubiF"
                     /locus_tag="OO372_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6340733.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4439..5863
                     /gene="miaB"
                     /locus_tag="OO372_001179"
     CDS             4439..5863
                     /gene="miaB"
                     /locus_tag="OO372_001179"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6340734.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDMAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6016..7056
                     /locus_tag="OO372_001180"
     CDS             6016..7056
                     /locus_tag="OO372_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6340735.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7053..7520
                     /gene="ybeY"
                     /locus_tag="OO372_001181"
     CDS             7053..7520
                     /gene="ybeY"
                     /locus_tag="OO372_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6340736.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7610..8488
                     /gene="corC"
                     /locus_tag="OO372_001182"
     CDS             7610..8488
                     /gene="corC"
                     /locus_tag="OO372_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6340737.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8513..10051
                     /gene="lnt"
                     /locus_tag="OO372_001183"
     CDS             8513..10051
                     /gene="lnt"
                     /locus_tag="OO372_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6340738.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10129..12006)
                     /locus_tag="OO372_001184"
     CDS             complement(10129..12006)
                     /locus_tag="OO372_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR6340739.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12461..13369
                     /gene="gltI"
                     /locus_tag="OO372_001185"
     CDS             12461..13369
                     /gene="gltI"
                     /locus_tag="OO372_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6340740.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13539..14279
                     /gene="gltJ"
                     /locus_tag="OO372_001186"
     CDS             13539..14279
                     /gene="gltJ"
                     /locus_tag="OO372_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6340741.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14279..14953
                     /gene="gltK"
                     /locus_tag="OO372_001187"
     CDS             14279..14953
                     /gene="gltK"
                     /locus_tag="OO372_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6340742.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14953..15678
                     /gene="gltL"
                     /locus_tag="OO372_001188"
     CDS             14953..15678
                     /gene="gltL"
                     /locus_tag="OO372_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6340743.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15796..16731
                     /gene="rihA"
                     /locus_tag="OO372_001189"
     CDS             15796..16731
                     /gene="rihA"
                     /locus_tag="OO372_001189"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6340744.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16823..18493
                     /gene="hscC"
                     /locus_tag="OO372_001190"
     CDS             16823..18493
                     /gene="hscC"
                     /locus_tag="OO372_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR6340745.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18553..20004)
                     /gene="djlC"
                     /locus_tag="OO372_001191"
     CDS             complement(18553..20004)
                     /gene="djlC"
                     /locus_tag="OO372_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6340746.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(20001..20708)
                     /locus_tag="OO372_001192"
     CDS             complement(20001..20708)
                     /locus_tag="OO372_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6340747.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20810..21364
                     /locus_tag="OO372_001193"
     CDS             20810..21364
                     /locus_tag="OO372_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6340748.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21374..22801)
                     /locus_tag="OO372_001194"
     CDS             complement(21374..22801)
                     /locus_tag="OO372_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6340749.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22798..23505)
                     /locus_tag="OO372_001195"
     CDS             complement(22798..23505)
                     /locus_tag="OO372_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6340750.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23669..24646
                     /locus_tag="OO372_001196"
     CDS             23669..24646
                     /locus_tag="OO372_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6340751.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24716..25198)
                     /locus_tag="OO372_001197"
     CDS             complement(24716..25198)
                     /locus_tag="OO372_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6340752.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25433..28015
                     /gene="leuS"
                     /locus_tag="OO372_001198"
     CDS             25433..28015
                     /gene="leuS"
                     /locus_tag="OO372_001198"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6340753.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28030..28611
                     /gene="lptE"
                     /locus_tag="OO372_001199"
     CDS             28030..28611
                     /gene="lptE"
                     /locus_tag="OO372_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6340754.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28611..29642
                     /gene="holA"
                     /locus_tag="OO372_001200"
     CDS             28611..29642
                     /gene="holA"
                     /locus_tag="OO372_001200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6340755.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29644..30285
                     /gene="nadD"
                     /locus_tag="OO372_001201"
     CDS             29644..30285
                     /gene="nadD"
                     /locus_tag="OO372_001201"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6340756.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30309..30920
                     /locus_tag="OO372_001202"
                     /pseudo
     CDS             30309..30920
                     /locus_tag="OO372_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31180..31497
                     /gene="rsfS"
                     /locus_tag="OO372_001203"
                     /gene_synonym="rsfA"
     CDS             31180..31497
                     /gene="rsfS"
                     /locus_tag="OO372_001203"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6340757.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31501..31968
                     /gene="rlmH"
                     /locus_tag="OO372_001204"
     CDS             31501..31968
                     /gene="rlmH"
                     /locus_tag="OO372_001204"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6340758.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31999..33900
                     /gene="mrdA"
                     /locus_tag="OO372_001205"
     CDS             31999..33900
                     /gene="mrdA"
                     /locus_tag="OO372_001205"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6340759.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33903..35015
                     /gene="mrdB"
                     /locus_tag="OO372_001206"
                     /gene_synonym="rodA"
     CDS             33903..35015
                     /gene="mrdB"
                     /locus_tag="OO372_001206"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6340760.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35026..36114
                     /gene="rlpA"
                     /locus_tag="OO372_001207"
     CDS             35026..36114
                     /gene="rlpA"
                     /locus_tag="OO372_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6340761.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36253..37464
                     /gene="dacA"
                     /locus_tag="OO372_001208"
     CDS             36253..37464
                     /gene="dacA"
                     /locus_tag="OO372_001208"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6340762.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37574..37837
                     /locus_tag="OO372_001209"
     CDS             37574..37837
                     /locus_tag="OO372_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6340763.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37938..38579
                     /gene="lipB"
                     /locus_tag="OO372_001210"
     CDS             37938..38579
                     /gene="lipB"
                     /locus_tag="OO372_001210"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6340764.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38839..39792
                     /locus_tag="OO372_001211"
     CDS             38839..39792
                     /locus_tag="OO372_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6340765.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            40001..40966
                     /gene="lipA"
                     /locus_tag="OO372_001212"
     CDS             40001..40966
                     /gene="lipA"
                     /locus_tag="OO372_001212"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6340766.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10300 a        10449 c        10292 g         9972 t
ORIGIN      
        1 gcccccgtaa aatataacca ttcaataaaa cccgctaaaa attatcgatt catcaataaa
       61 tacatcctca caccagaccc tttttcacca atcgacaatc accattacgt tttttatttt
      121 ttcaccgcca aatcgcttgc gcacagtgct gctttgcggc atttttttaa acaagcaaac
      181 acaacaagca acaaatacca ggttaacgga gaaggttatg tgttcaattt ttggcgtatt
      241 cgatatcaaa acagacgcag ttgagcagcg taagaaagca ctcgagctgt cacgcctgat
      301 gcgtcatcgt ggcccggact ggtccggtat ttatgccagc gataacgcca ttctcgctca
      361 cgaacgtctg tcaattgttg acgttaacgc gggggcgcaa cctctctaca accaacaaaa
      421 aacccacgta ctggcggtaa acggtgaaat ctacaaccac caggcattgc gcgctgaata
      481 tggcgatcgt tatcagttcc agacagggtc tgactgtgaa gtgatcctcg cgctgtatca
      541 ggaaaaaggg ccggaatttc tcgacgactt gcagggcatg tttgcctttg ccctgtacga
      601 cagcgaaaaa gatgcctacc tgattggtcg cgaccatctg gggatcatcc cactgtatat
      661 ggggtatgac gaacacggtc agctgtatgt ggcctcagaa atgaaatcgc tggtgccagt
      721 ttgccgcacg attaaagagt tcccggcggg gagctatttg tggagccagg acggcgaaat
      781 ccgttcttat tatcatcgcg actggttcga ctttgatgca gtgaaagata acgtgaccga
      841 caaaaacgag ctgcgtcagg ctctggaaga ttccgttaaa agccatctga tgtctgatgt
      901 gccttacggt gtgctgcttt ctggtggtct ggattcctca attatttccg ctatcaccaa
      961 gaaatacgca gcccgtcgcg tggaagatca ggaacgctct gaagcctggt ggccccagtt
     1021 acactccttt gctgtaggtc tgccgggttc accggatctt aaagcagccc aggaagtggc
     1081 aaaccatctg ggcacggtgc atcacgaaat tcacttcact gtacaggaag gtctggatgc
     1141 catccgcgac gtgatttacc acatcgaaac ttatgatgtg accactattc gcgcttcaac
     1201 accgatgtat ttaatgtcgc gtaagatcaa ggcgatgggc attaaaatgg tgctgtccgg
     1261 tgaaggttct gatgaagtgt ttggcggtta tctttacttc cataaagcgc ccaacgctaa
     1321 agaactgcat gaagagacgg tgcgtaaact gctggccctg catatgtatg actgcgcgcg
     1381 cgccaacaaa gcgatgtcag cctggggcgt ggaagcacgc gttccgttcc tcgacaaaaa
     1441 attccttgat gtggcgatgc gcattaaccc gcaggataaa atgtgcggta acggcaaaat
     1501 ggaaaaacac atcctgcgtg aatgttttga gtcatacctg cccgcaagcg tggcctggcg
     1561 gcagaaagag cagttctccg atggcgtcgg ttacagttgg atcgacaccc tgaaagaagt
     1621 ggcggcgcag caggtttctg atcagcaact ggaaactgcc cgcttccgct tcccgtacaa
     1681 cacgccaacc tcaaaagaag cgtatctgta ccgggagatc tttgaagaac tgttcccgct
     1741 tccgagcgcc gctgagtgcg tgccgggcgg cccgtccgtc gcgtgttctt ccgctaaagc
     1801 gattgagtgg gatgaggcgt tcaagaaaat ggacgatccg tctggtcgtg cggttggcgt
     1861 tcaccagtcg gcgtataagt aagaaatgcg aaacagcccc ggtgaaatcc ggggctgttt
     1921 cagttattga ggtgcttgtt gattctgata tgcagtaagg aaatgttccg ggtttgccac
     1981 atcactgttt gacaactcat cactgtaggc cagtatttca tagattaatt tcccaccacc
     2041 attgttgaat tccgaatttc atcctgtatg acaaaagaat ggctgtaggg tggggaaaaa
     2101 acacaaagta aacaataatt gacgaatata gcgccacgct gttcgcaacc taaccaaaca
     2161 gtcactttcg agcaattttc cttgaaaaag aggttgacgc tgcaaggctc tatacgcata
     2221 atgcgccccg caacgccgat aaggtatcgc gaaaaaaaga tggctacgta gctcagttgg
     2281 ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccatc
     2341 ttttttgcgg gagtggcgaa attggtagac gcaccagatt taggttctgg cgccgcaagg
     2401 tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga aagcgttgta cggatggggt
     2461 atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg aatccaggta
     2521 ccccagccat cttcttcgag taagcggttc accgaccggt tattggggta tcgccaagcg
     2581 gtaaggcacc ggtttttgat accggcattc cctggttcga atccaggtac cccagccatc
     2641 gaagaaacaa tctggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
     2701 tcacaggttc gaatcccgtc gtagccacca aattctgaat gtgtcgaata cgttcggcaa
     2761 attcaaaaac caatttgttg gggtatcgcc aagcggtaag gcaccggatt ctgattccgg
     2821 cattccgagg ttcgaatcct cgtaccccag ccaatttatt caagacgctt acatcgtaag
     2881 tgcaccagtt ggggtatcgc caagcggtaa ggcaccggat tctgattccg gcattccgag
     2941 gttcgaatcc tcgtacccca gccacattaa aaaagctcgc ttcggcgagc tttttgcttt
     3001 tctgcgttca ttcaatgtcg aatgcgatgt tgacacgtct tatccttcaa tgtcggatgc
     3061 gacgctgccg cgtcttatcc gacctacggt tggcacgcat ccggcaatgt tgtaaggcta
     3121 caaccctaac gcatatttca gcgcctgacg tttcaacacg ccagcacgct ctgccgccat
     3181 taacccgaga ttacgcacaa aacgcagcgg cggcagatta ttactgaatc cggcataaaa
     3241 cagatccata ccactttgca taatgaagtt atccgccatg cgccgcatct ggtaacgctt
     3301 gaggacagga taactggccc acgcttcacc gtagctgcgg gcattcacca gaacatcaat
     3361 cagggcatcg acatcacgat aaccaagatt caccccctgc cccgccagcg gatggatggt
     3421 atgcgcggca tcgcccacca gcgcaagccc cggctgcaca tactgcaacg catgtcggcg
     3481 cgtcagcgga aacgcaccag cggcaagcgg tgtaacgtaa cccagacgcg acgggaaatg
     3541 cttcgcgatt tccgcctgga attgcgccat attcatattc tgcaactggc gaatacgcgc
     3601 cggagagtca taccacacca gcgatgccca gttatcaaac aacggcagaa acgcacgcgg
     3661 tccgtccggg gtaaattgct gccaggtgct gtcgccggga tcgttctcac actgtacgct
     3721 aatcaacatg cacgactgcg catactgcca ggcatgaacg ccaattcccg ccatctgccg
     3781 cacctgagaa tttgcgccgt cggcaccaat caccagtttc gcgcggatcg tttcaccgcc
     3841 cttcagttcc agctcctgaa gatcattatc tcgatgtaat gcaatcagcg agcctggcac
     3901 acgtaacgtt actttcggat gcgcttccag cgcctgccac agcgcctgtt gcaggacggt
     3961 gttttccacc atatagccaa gcaacggtag cttaagttca gcggcgtcaa acaccacatg
     4021 cgccgtttcc cactcccacg tttccagtct gcggtaagga tggcaacgca tagcctgtac
     4081 tgcatcccag acccctaacc ctttaagcaa tgataccgat gcggcgctga tcgccgagat
     4141 ccgcacgtcc ggttggctat cagcgacaaa cggtgcaggt tctgcgtgct cgatcaccgt
     4201 taccgaaaat ccgtgctgtg ccagccccag cgccagcgcg ccgccgacca ttcctccgcc
     4261 gacaatggca atttccgttg gttgatttgt catggtcaat catcctgtta gcaatatgct
     4321 ttaagtttac aggattttta cccccaaggg ctgataacgc tcatactggt cacaacggca
     4381 gcaaagcatt acactatacg ccctgcattc ctggctacta tttcgcaaga gcaagtcgat
     4441 gaccaaaaaa ctccatatta aaacctgggg ctgtcagatg aacgagtacg attcatcgaa
     4501 gatggccgat ctgctggatg ccacccacgg ctatcaactg accgacatgg cggaagaagc
     4561 ggatgtgctg ctgctgaaca cctgctcaat ccgcgagaag gctcaggaaa aagtcttcca
     4621 tcagttgggt cgctggaaac tgttaaaaga gaagaatcca gacctgatta tcggcgtcgg
     4681 tggctgcgtg gcatcgcaag aaggcgagca cattcgccag cgcgcccact atgtcgatat
     4741 tatttttggg ccgcaaacgc tgcaccgtct gccggagatg atcaactccg tgcgcggcga
     4801 ccgcagcccg gttgtagata tcagcttccc ggaaatcgag aagtttgacc gtctaccgga
     4861 accgcgcgcc gaagggccga ccgcgtttgt ctccatcatg gaaggctgca ataaatattg
     4921 cacctactgc gtggtgcctt acacccgtgg tgaagaggta agccgtccgt ctgacgatat
     4981 tctgtttgag attgcccagc ttgcggctca gggcgtgcgt gaagtcaacc tgctcggtca
     5041 gaacgtgaac gcctggcgtg gtgaaaacta cgacggctcc accggatcgt ttgccgatct
     5101 gctgcgtctg gttgctgcga tcgacgggat cgatcgtatt cgctttacca ccagccatcc
     5161 gatcgaattc accgacgata tcatcgaagt gtatcgcgac acgccggagc tggtgagctt
     5221 cctgcatctg ccagtacaaa gcggttccga tcgcattctg aacctgatgg gccgtactca
     5281 cacggcgctg gagtacaaag cgatcatccg taaactgcgt gcggcgcgtc cggatattca
     5341 gatcagttct gacttcattg ttggcttccc tggcgaaacc accgaagact tcgagaaaac
     5401 gatgaagctg attgccgacg tcaatttcga catgagctac agctttatct tctctgcacg
     5461 tccgggtaca ccagccgccg atatggttga tgatgttccg gaagaagaga agaagcaacg
     5521 tctgtatatt ctgcaagaac gtatcaatca gcaagcgatg gcatggagcc gccggatgct
     5581 cggcaccacc cagcgtattc tggtagaagg gacttcgcgt aagagcatca tggagctttc
     5641 cgggcgtacc gaaaataacc gcgtggtcaa cttcgagggc acaccggata tgatcggtaa
     5701 attcgtcgat gtagaaatta ccgacgtcta cccgaactct ctgcgcggta aagtggtgcg
     5761 tactgaagat gaaatgggtc tgcgcgtggc agaaacaccg gaatcggtga ttgcccgtac
     5821 ccgcaaagaa aacgaccttg gcgtgggtta ttatcagccg taattctcag gcccgccgtt
     5881 ccggtgggcc ttgttttttc tctctttatc cccatcttta tcgcaatgct tgcgcctttt
     5941 tcctgtggcg tgaataattt ccttataggc cggtgaatgt tcagcttccg gcggcataca
     6001 gataagagga acggtttgaa catagacact cgcgaaatca ccctggagcc agcagacaac
     6061 gcgcgtctgt tgagcctgtg cggcccgttt gatgacaaca tcaagcagct cgaacgccgt
     6121 ctcggcatcg agatcaatcg ccgcgacaac cactttaaac tgaccggccg tccgatttgc
     6181 gtcaccgctg cggcagacat tctgcgtagc ctgtatgtcg atacagcccc gatgcgcggt
     6241 cagattcagg atatcgaacc ggaacagatc caccttgcga ttaaagaagc gcgagtactg
     6301 gagcaaagtg cggagagcgt gccggagtac ggcaaagcgg tcaatatcaa aaccaaacgc
     6361 ggcgtaatta agccgcgcac gccaaaccag gcgcagtaca tcgccaatat tctcgaccat
     6421 gacattacct tcggcgttgg cccggcgggt acgggtaaaa cctacctggc agtggctgcg
     6481 gcagttgatg ccctggagcg tcaggagatt cgccgtattc tgctgactcg tccggcggtc
     6541 gaagccggtg agaaactggg cttcctgcct ggcgatttaa gccagaaagt agacccgtat
     6601 ctgcgcccac tgtacgacgc gctgtttgaa atgctgggct ttgagaaagt tgagaaactg
     6661 attgagcgca acgttattga agtcgcgccg ctggcctata tgcgtggtcg tacgctgaac
     6721 gacgcgttta tcattctcga tgagagccag aacaccacca tcgaacagat gaagatgttc
     6781 ctgacccgta tcggttttaa ctcaaaagcg gttatcaccg gcgacgtcac acagatcgac
     6841 ctgccgcgta atactaaatc aggcttacgt cacgccatcg aagtgctggc cgatgtcgaa
     6901 gagatcagct ttaacttctt ccacagcgaa gacgtggttc gtcaccctgt ggtggcgcgt
     6961 atcgttaacg cctatgaagc ctgggaagaa gccgaacaaa aacgaaaagc ggcgctggcg
     7021 gcagaacgca agcgcgaaga acaggaacaa aaatgagtca ggtgatcctc gatttacaac
     7081 tggcatgtga agataattcc gggttaccgg aagagagcca gtttcaaaca tggctgaatg
     7141 cggtgatccc gcagtttcag gaagaatcgg aagtgactat tcgcgtggtc gataccgccg
     7201 aaagccacag tctgaatctg acctatcgcg gtaaggataa gccgaccaac gtgctctcct
     7261 tcccgtttga agtgccgcct ggcatggaaa tgtcgctact gggcgatctg gttatctgcc
     7321 gtcaggtggt tgagaaggaa gctcaggagc aaggcaaacc actggaggcg cactgggcgc
     7381 atatggtggt gcacggcagt ctgcatttgt taggttacga tcacatcgaa gatgacgaag
     7441 cagaagaaat ggaagccctc gaaacagaga ttatgcttgc tctgggctat gaggatccgt
     7501 acattgccga gaaagaataa tttgccaggc atttgactgg cagacgtccc cgccgttggt
     7561 gattaacgac gacgttttga ttaactaaca tgagaaccca tacgacgcca tgagcgacga
     7621 caattcacac agtagtgaca cgataagcaa caagaaggga tttttctccc tgttactcag
     7681 ccaacttttc cacggtgaac cgaaaaaccg tgacgaactg ctggcgctga tccgtgattc
     7741 cgggcagaac gaccttatcg acgaagatac gcgcgatatg ctcgaagggg tgatggacat
     7801 cgcagaccaa cgcgttcgcg acatcatgat cccccgctcc cagatgatta ccctgaaacg
     7861 caaccagacg ctggacgaat gccttgatgt catcatcgag tccgcccact cacgtttccc
     7921 ggtgattagc gaagacaaag atcacattga agggattctg atggcgaaag acttgctgcc
     7981 gtttatgcgc agcgatgctg aagccttcag catggacaaa gtgttacgtc aggcggttgt
     8041 cgttcctgaa agtaagcgcg tagaccggat gctgaaagag tttcgctctc agcgttacca
     8101 catggcgatc gtcattgacg aattcggtgg ggtttccggt ctggtgacca ttgaagacat
     8161 cctggaactg attgttggtg agattgaaga cgagtatgac gaagaagatg atatcgactt
     8221 ccgtcagctg agtcgtcata cctggaccgt gcgtgcactg gcttccattg aagacttcaa
     8281 cgaagcgttc ggcacccact ttagcgatga agaggttgac actatcggtg gtctggtgat
     8341 gcaggcattt gggcatcttc cggcgcgtgg cgaaactatc gacatcgacg gttaccagtt
     8401 caaagtggcg atggccgaca gtcggcgtat tattcaggtt catgtcaaaa tcccggatga
     8461 ctcaccccag ccgaagctgg atgaataaaa ccgaaactgg atagataact acatggcttt
     8521 tgcctcatta attgaacgcc agcgcattcg cctgctgctg gcgttattat tcggtgcctg
     8581 cggaacgctg gccttctctc cttacgacgt ctggcctgcg gcgattattt cgttgatggg
     8641 gcttcaggcg ttgaccttta accgccgtcc actccagtct gccgctattg gcttttgctg
     8701 gggatttggc ctctttggca gcggtattaa ctgggtctat gtcagcatcg cgacctttgg
     8761 cggaatgcct ggcccggtta acatcttcct ggtggtgctg ctggcggcgt atttgtcgct
     8821 gtataccgga ctgtttgctg gcgtgctgtc gcgtctgtgg ccgaaaacca cctggctgcg
     8881 cgtggcgatt gccgcccctg ctctctggca agtgaccgag tttctgcgcg gttgggtact
     8941 gacaggcttc ccgtggttac agttcggcta tagccagatt gatggcccgt taaaagggct
     9001 ggcaccgata atgggcgtgg aagccattaa cttcctgctg atgatggtta gtggcctgct
     9061 ggcactggcg ttggtcaaac gcaactggcg tccgctggtg gtggccgtcg tgctgtttgc
     9121 cctacccttc ccgctgcgtt acatccaatg gtttaccccg caaccggaga aaaccattca
     9181 ggtttcgatg gttcagggcg atattccgca atcactgaaa tgggatggag atcagcttct
     9241 taatacgctg aagatttact acaacgcaac ggctccgctg atgggcaaat catcgttgat
     9301 tatctggccg gagtcggcga taaccgatct ggaaattaat cagcaaccgt tcctcaaagc
     9361 actggacggt gagttgcgcg ataaaggtag ctcgctggta accgggattg tcgacgcgcg
     9421 tctcaataag cagaaccgct acgataccta caacaccatc atcacactgg gtaaaggtgc
     9481 accatacagc tacgaatcag ccgatcgcta taacaaaaac catctagtgc cgtttggcga
     9541 gtttgtcccg ctggagtcga ttctgcgtcc gttagcaccg ttctttgatc tgccgatgtc
     9601 gtcgttcagc cgtgggccat atatccagcc gccgctgtcg gtaaatggta ttgagcttac
     9661 tgcggctatt tgctacgaga tcattctcgg cgagcaagtg cgcgataact tccgcccgga
     9721 taccgactat ctgctgacta tctccaacga tgcgtggttt ggtaagtcta ttggtccatg
     9781 gcagcacttc cagatggcgc gaatgcgtgc gctggagctg gcgcgcccac tgttgcgcag
     9841 caccaacaac ggcattacgg cggtgattgg cccgcagggg gaggttcagg cgatgatccc
     9901 gcagttcacc cgcgaggtgt tgaccactaa tgtgacaccg accaccggac tcacaccgta
     9961 tgcacgtacc ggcaactggc cactgtgggt gctgacggcg ctgtttggtt ttgccgctgt
    10021 gttgatgagt ctgcgagcac gtaaacgttg atgtgcctga tgtatcctgc atgagcaaaa
    10081 cgtactttct cattgagctt tggggcatgg ttatcatgcc cctttttctt acggttcagg
    10141 gatacaagcc acattgccat attggggatg acgaataatt gcctgcggat caagcgagcg
    10201 cgtcctgtcc atttggatca acccgttctt cgccatgaca aatatttttc taccatccgc
    10261 tgaaaaacga aggtgattaa cgtagctggc aggataaacg ccaataatct gcccggcatt
    10321 aatattgagc aggatgacat aaccttgcag gccatatgac accaacgcta aatattgacc
    10381 gtccgggcta ataccctcaa cggaataaac gctcctgcta caccattgcg tcataatgct
    10441 ggcttgttga tcccctacgc ttatacgtga aatcccctga ctatcggcaa aaataacctc
    10501 gccgttttca ggtcgaaaac gcatcgccga tatcgttcca cgcgcatcac ctgcgggaaa
    10561 gtcttctttc tgatgcgtga tggtattgat ccgcacgatg ttacctctgc ctgctcccgg
    10621 aaccagtaat gttctttcat ccggggaaag tgccataacc caggcgcctg gtttaccgct
    10681 cgtccccgtc ccgtcgtatt tctccagcgg gatatccgcc acatgctgat aagtcaccag
    10741 atcaatcgct ggcacactat tactggtgcg attcatcacc caggcttttg tgccatcacg
    10801 cgagaggata aggctgtcag gatgtgcacc ggtggtaatc gactgaataa tctcccctgt
    10861 cgccacgtta atgaccgata acgcattcgc ttcaaatgaa gtcgcataaa tcaacttttc
    10921 atccgggctt aatgccagcg atctgacgcc ctgatcttta cagccaccac aatgtttgac
    10981 tgacttttct ttcgctatgg gcgctgtaaa ggtatgcagg attttctttt gtccgaggtc
    11041 gaaaacaaca atgctgttcg tgttttcaat tgcggcatac aacatattac catcccgact
    11101 gatcgccata tcatggatat cggcaatctc agcgagcaaa tcaccactgg cctgttcacg
    11161 acttaccgga gcggataatg tttccctgag ggcgttgcgt tcctccgcta actgctgctt
    11221 tttttgccgt cgctcttgtt caatctcctg tggataaaac gcttcacgca ggctttgcct
    11281 gacctgtaaa actgactcat ccatctgctg ctgcgcaagc cagatagccc cggtcagaag
    11341 aagactgccc gcaacaataa tcccgccttc cgtaacgagt cggttttgcc cagataaacg
    11401 cgcgctcagc caacccaacg cgccacccgc aatcaggccg ccaatgtggc aagcgttatc
    11461 tattcctgtt tgccgggcac cgtacaacag tgtcagcgcc accataccca acagaggaaa
    11521 tactcgccgc tggtttttat ggtatgtgcc cgtacctgaa gcaaactgtg ttgctatcgc
    11581 tgcaccagct attcccagaa tcgccccgga tgccccaacg ctgatgacta tctgctcact
    11641 ttcgcgtaac gcataacagg cgctgatgag cccaccaaag acgccggaga ataaccaggt
    11701 aatcaggagt cgccatttcc ccagaatctg ttccgccacg cctcccacga ccaggaatgc
    11761 aaacatattc atcagcagat gagaaacact actgtgcaga aagatactgc tcaataagcg
    11821 ccagggttca cccgacaaag taagaggcgc aacgtttgcg ccgagatgga taagtgcgga
    11881 ttgatcgacc tcttcataac tcatccattg tgcgagataa caaaatatcc caatacagag
    11941 tgcaaaaagg ataagggtta ttgcgggtaa ttgaacattt aacggcttaa ccgaagatgc
    12001 agacataagg tgtttcttcc ttgaaaagac aggtaaaaag catttttccc gtgtgcagtt
    12061 atcatcccgg caaacgtccc atgccgaaaa gataattctg tccgaaatat gactttttca
    12121 gcctaaaatt acaatcgtaa ttaattcgcc cctaagtatt ttttggcatg taagttgctt
    12181 tatttactgt gtaagcgatg aaatcagccg cgacgcaacc aggttgcacc accacaaatc
    12241 agcggtagca ccaaaaaggt gcggtttaag aattttgctt cggaatggtg cggcgcatct
    12301 cgcaaaaaca aacaataaaa cagcaatatc tttacattta gccaaactaa atgttaccta
    12361 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    12421 tatccatgcg ttcttaacgc agaagataaa ggagttggat atgcaattac gtaaacctgc
    12481 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    12541 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    12601 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    12661 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    12721 ggtaaaactg attccgatta cctcacaaaa ccgtattcca ctgctgcaaa acggcacttt
    12781 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    12841 tgatactatt ttcgtggtcg gtacgcgcct gctgaccaaa aagggcggcg atatcaaaga
    12901 ttttgccgac ctgaaagaca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    12961 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    13021 cagtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgatgc
    13081 cctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggaaa tcgtcggcaa
    13141 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    13201 gctgatggat gacaccaccg cgcaggtgca gacctccggt gaagcggaaa aatggtttga
    13261 taaatggttc aaaaatccca ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    13321 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    13381 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    13441 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    13501 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    13561 gtattttttt acaacaagcc ccgttcggca acaccaccta tcttggttgg atctggagtg
    13621 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    13681 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    13741 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    13801 tcccggagct gttgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    13861 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    13921 aacaggtacg cgccgccatt cagtcgttgc cgcgtgggca aaaaaatgcc gcgctggcga
    13981 tgggcctgac gctaccgcag gcttatcgat acgtactgct gcctaatgct tatcgcgtta
    14041 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    14101 ctattggtct ggtggatatg gcggcgcagg cgggtaaatt gcttgattac tcagcccacg
    14161 cctgggagtc gtttaccgct attacgctgg cgtatgtgtt gattaacgcg gttatcatgc
    14221 tggtaatgac gctggttgaa cgtaaagttc gcctgcctgg caacatgggg ggcaaataat
    14281 gtacgagttt gactggagtt ccattgtccc ttccctgcca tatctgctcg acgggctggt
    14341 gatcacgctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gcaccatgct
    14401 ggcggtgatg cgcttgtcca gctttgcgcc cgtagcgtgg tttgccaaag cctacgttaa
    14461 cgtattccgc tcgattcctt tagttatggt tttgctgtgg ttttacctga tcgtgccagg
    14521 ttttctgcaa aacgtgctgg gattatcacc aaaaaatgat atccgtttaa tttcggcgat
    14581 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ctggtattca
    14641 aagtatttct cgtggtcagt cgagcgccgc gctggcgttg ggaatgactc actggcagtc
    14701 gatgaaactg attattctgc cgcaggcgtt ccgcgcgatg gtgccgcttc tgctcactca
    14761 gggcatcgta ctgttccagg atacctcact ggtgtatgtg ttatctctgg ccgatttctt
    14821 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gttgagatga tcctgtttgc
    14881 cggatttgtt tatttcgtta ttagtcttag cgcgtcgttg ttggtcagct acttgaaaag
    14941 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    15001 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    15061 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    15121 gaaatcaccg tcgatggtat cgtggttaat gacaagaaaa ccgatctggc aaagctgcgt
    15181 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    15241 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    15301 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    15361 ctttccggcg gtcagcagca gcgtgtggca atcgctcgcg cattgtgtat ggatcctatt
    15421 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    15481 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    15541 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    15601 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcgaa agacttcctc
    15661 gcgaaaatcc tgcattaatc actctggcgc gcatacccaa gtgcgcgcca tctcacgctt
    15721 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    15781 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgacgac
    15841 gctatcgcaa tagttctcgc cctcgcctca ccagagcttg atgtcaaagc aattacgtct
    15901 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    15961 cttaatcgca ccgatattcc ggtagcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    16021 attatcgcgg acaatgtgca cggcgaaagc ggtctggatg gcccggcatt accggaaccg
    16081 acgttcgcac cgcaaaactg tacggcggta gagctgatgg cgaaaacgct gcgcgaaagt
    16141 gcggaacctg tcacgattgt gtctaccgga ccgcaaacta acgttgcctt gctgctcaat
    16201 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    16261 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    16321 attgtcttcc agtcagggat cccagtggtg atggccggtc tggatgttac tcacaaagca
    16381 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    16441 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    16501 ggtgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    16561 actgttgagc gctgggttgg cgtggaaaca caggggaaat atagccaggg tatgacggtt
    16621 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaatggt cgatgttgat
    16681 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acacacgtca
    16741 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tgtttatctg tttatctctt
    16801 tttctttcaa gatataaatg caatggataa tgcagaactc gccattggta tcgatctcgg
    16861 cactaccaat agtttaattg ccgtctggaa agacggtgcc gcgcaattaa ttccaaataa
    16921 gttcggtgaa tatttaacac catccataat tagcatggat gaaaataatc atattttagt
    16981 cggaaaaccg gctgtatcac ggcgtacttc gcatccggat aaaacggcag cgttatttaa
    17041 acgtgcaatg ggcagtaata ccaactggcg gttaggcagc gacacattta acgcgccaga
    17101 actgtcctct ttggtattac gctcattaaa agaagatgcc gaagaatttc tgcaacgtcc
    17161 gattaaagat gtggtgatct ccgttccggc ttatttcagc gatgaacaac gcaagcatac
    17221 ccgtttagca gcggagttag ccgggttaaa tgcggtacgc ttaattaatg aacccacagc
    17281 agctgcgatg gcatatggcc tgcatacgca acaaaatacc cgctcgctgg tttttgatct
    17341 cggtggcggc acgtttgacg tcacggtgct cgaatacgcc acgccggtga ttgaagttca
    17401 cgcctccgct ggcgataact tcctcggtgg cgaagatttt acccatatgt tggtcgatga
    17461 cgttttaaag cgcgcggatg tcgccaggac cacgcttaac gagagtgaac tggcagcctt
    17521 gtacgcctgt gtggaagcgg caaaatgtag caatcaatcg ccattgcaca ttcgctggca
    17581 gtaccaggat gaaacgcggg agtgcgaatt ttacgagaac gaactggaag atttgtggtt
    17641 gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg ttgcgcgatg cacgtctgaa
    17701 gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg tcacaaatgc cgctggtgca
    17761 gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa agttacgacc cgagcaccat
    17821 tgtcgcgctg ggcgcagcaa tccaggccgc ctgccgctta cgcagtgaag atattgaaga
    17881 ggtgatcctc actgatattt gcccttactc gttgggcgtt gaagttaacc gccagggcgt
    17941 ttccggcatt ttctcgccaa ttattgaacg aaacaccaca gtacccgtgt cgcgtgtaga
    18001 aacttactcg accatgcacc cggaacagga ttctattacg gttaacgtct atcagggaga
    18061 aaaccacaaa gttaaaaata acattctggt tgaatccttc gatgtgccgt tgaagaaaac
    18121 cggggcttat cagtcgattg atattcgctt tagttatgat atcaacggct tgcttgaagt
    18181 tgacgtgctt ctggaagatg gcagcgttaa gtccagagtc attaatcata gcccggtaac
    18241 attgagcgcg cagcagattg aagagagtcg gacgcggtta tccgcattga aaatttatcc
    18301 gcgcgatatg ctaatcaacc gcacctttaa ggccaaactg gaagagttgt gggcgcgggc
    18361 gctgggtgac gagcgagaag agattggccg ggtgatcacc gattttgatg cggcgttgca
    18421 gtcaaacgat atggcccgcg tcgatgaagt tcgtcgacgg gcgagcgatt atttggctat
    18481 tgagatccct taaacacggc tgccggatgc ggcataaacg ctgcatccgg catcggcgca
    18541 aaaaacacat cctcagctaa acaacatcgc gatttttccg agcgcataac ctatcatcag
    18601 caccgttaag ctgattttga taatactggt aatgctctgt ggtttttcat cctcatcatt
    18661 tttttcttca tggttttcag gtgttggctc atcgacatct tcatcctccg gctcgagttt
    18721 cactttcgtc aacagcatct gggtgataat ggcctctaat ggatgagcat ctccaccttc
    18781 cagccttgta ttaagacggg ccagcatctc ttgtttcggg aacgcttcca gcacaacctg
    18841 ttccgctacc cgatacaaat ccagtaacgg tgaatcttgc tctgtctcgc taccgactct
    18901 cgatttccac cacgcccatg ctttgcgctt gaagaataac gactcaataa acgattgacc
    18961 taacggcgat aattttccgc tatgtaaaat ctccgcccat aacagccgcg tccgggcgct
    19021 ctcaccagga acatatttca acggtttacc ttgcgcatct accatgcttc gcgcttcgat
    19081 aaccatcact aataacgcga aataatccgg gcaatgacgc ttgctccact gcaacaacaa
    19141 attatcagct tgtgtagatg ggtaactttc ccagtacgcg cagagatcgg caagcaggct
    19201 ttccccctcg ccgcagtaca aacgttgtgc acactggtaa taccgggcat cttcgctttc
    19261 tggttcttcc gcttgccact gtttggctac aggaatgagt tccgccatac ctgtttgcac
    19321 cgagctgtac cagcgcaaca gtttgcgatg taactttgca tcatcaggaa tgaaccacgg
    19381 tccttccatc gccaaaaacg cagtgacata gtcaggatgg taacggcagc aacgttccag
    19441 cgcgaagtaa tattcaacag tctggttctg taacgcgacg ggatattcca gaaggctaag
    19501 gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa gcctcccttt cttcctcatc
    19561 cattccttcg ctgtcattaa acgaatgcca gttcaaacgc tctgccaaca aagaagcaca
    19621 atttaatgaa agatatcgcg cttctatagc gattgcacac agcggccagc gtaattgatc
    19681 gacatcctcc atgttccagg tatttaattg ctgaataaat ttctgccagg cggagggctg
    19741 aaaacgatca ctttctgaat ccagtaatgt cctgaatgca cgtagtattt catgttcagc
    19801 ggtggcatct ttttcttcat catcggcttc ctcgacagga tttaccgcca gtcgcagcgc
    19861 ctcttcatag gcctggcgta atgctttaaa cccctgcgga tcggtttcgg gatgacacaa
    19921 cggtaagcga gcaagataag cctggcggat aatgtctatt tgcgtcgtgc tttcaatttc
    19981 gaggatttgc caacatgttt tcataggtcg ctcacatcct taagcgttac cgggcattca
    20041 ggctggacag ttaatggttg ccagggaagc gtggcataga aacggtcgct tcccgctgcc
    20101 agctcttcga acatccgact cccggcgcta tcgtatttgt acagggttag cgcctcgcgt
    20161 agcgaagcgt tgtcttcgca ctgagaagat tgccagtaga gacgccctag tgaataggca
    20221 gaaaaatagt gcatccagct gtgatagtaa tggtgagctc gtgcatagac gcgcgattgc
    20281 agccataaac tctcttcttc cgtcagccag ttgttgagca caccgacccg tgacagaaac
    20341 cccattcgaa cataatccca ggctttgatt cccccttctc cacagcacat agcggtttgg
    20401 gcaacgaatt gggtataaat caaccctctc tccgagccac cagcaattaa tgctttcctc
    20461 tcttcagggg aagagcgttg ccatgtcaga tataagccag ctaaatcatt ggcatgacca
    20521 tcatcagtca tctgataaat agtctgaatt aaatcatcac gattatttat tccccaggat
    20581 gatgagagat cgaattcttt tatcccagta tacatttttg gcgagtcatg cgcgggtttg
    20641 caactctgat taagtatttc catgggcgca gaaagcgcaa aaagaagata ttgttcttcc
    20701 ttattcattt tttaataatc ccttaagtgc attaactgac tactttttta ttattgatga
    20761 gtataaactt gacgctttca aaataaaaag aaaatcgaag cattcacaca tgaataaaaa
    20821 atcaatgtat atattcgcaa tttttatagt tgcagcaatt acctgtatta gccaacccaa
    20881 gaaaacgacg ttgcgtgata aagccatggt gaattatgcc tttgattatt taagatcacc
    20941 aggcagtcta ccgttaacca cggcagccac ggagctttcc gcgattcatg gtcactcaac
    21001 atcgcaatat cgccttggcg aattttatct tcatggtagc gacggtaaac cactggatta
    21061 tacacaggcg agatactggt atgagcaatc agcagaacag gaaaatccgc gcgcacaaag
    21121 caaactgggg tggatctacc tgaaaggcct gggagtcaaa cccgataccc gtaaagcaat
    21181 tctctggtat aaggaagcag ctgaacaagg gtatgctcat gctcaatata ctttgggttt
    21241 gatctacaga aatggcactg gtattaatgt taaccattat gaatctcaaa aatggttaaa
    21301 actggccgcc aaacaacatt acaaaaatgc ggaaagatta cttgccggac ttcccgcaca
    21361 ttaacgtcga ctgtcacccg aagagatgga gtatttttcc tatcaaaccg gcaaagataa
    21421 aaatgtagaa aataattttg atgacgccca gacagcccag cccgccatcg tcaacgggca
    21481 ttggctgcgg ttgcggctcc ggctcgacgg tttcctgacc ggtaaaattc gccgcctgaa
    21541 ttaataatcg acgaataatt aatgcttcca gtggacaacg atcttctgca tcaaggcggc
    21601 tcacaatcgg cattaatgat tcttctgtcg gttggctggc ctgcactaat tgtttggcaa
    21661 ctaaatataa tggccataag ggatgttctg tatcacgcgt gagtgagatc cgcggcttac
    21721 gtcctttgtg caaaaccatc tcgaccagtg aacgccccag cgcagagagt ttttcatcgc
    21781 tgagtatttc gtataaatga aaacgcgtac gggcgctgtc gccggggaca taaagtagct
    21841 gtttaccctg atcgttaacc agttcacgag cttcaatcat catcaccact aacgggtaat
    21901 aatcgacccg atgctgacgg caccattgca atattaaagc atccgcctgg gtggagggat
    21961 actcgcgcca gtagtcacaa agttctgcca gcaagctttc gccttctcca caatagacgc
    22021 gttgcgcgta ttcataataa cgcgcatctt cattatcagg ttcagcttcc cgccacgacc
    22081 gggcgtaatc cagaagttcg gggatatcca ggcgacctgc gctataccag cggagtaaat
    22141 ttctgtgcag ttttgcatca tcggggatga tcactgcgcg atgttgcgct aaatacaatg
    22201 tcagccattc tggatgcgat gaccaaatcc gagcgaacat ttggtaaaaa tcgatggtgt
    22261 cattttgcac ggcaacgggc agatgcagaa tggtctggaa gttaaaaaca ttccccttcg
    22321 caatggcata taaaaaggat tccagttctt cttcgtctat ttccccgacg ttttcctcct
    22381 gcaaccaccg caatctttct gctaataaca ccacgcactc gaaggataaa tgggcagtgt
    22441 tcatggcgat tgtgcacagc gaccagcgta attcatcaat ctcttccatc gagcaataat
    22501 ttaattgctg aatgaatcgc tgccaggcgg agggcagaaa acgttcgcta tcagaggcaa
    22561 gtaacgcgcg aaaggcgagc agaatttcat gttctgctac ctcatattct tctggttgcc
    22621 aaacagattt agcaggcgac tgcgcaatcc ggagcgcctc ctcatacgcc tgacgaagtt
    22681 gtttaaaacc ctgcggatcg gtttctggat gaaaggacag taacagcgcc agataagcgc
    22741 ggcggataat atcgacgtca gtcgtttcct ctatttcgag gatcttccag caatttttca
    22801 taggtcgctc atatccttaa gcgtttccgg gcatgtgggg tagtcagcca gtggttgcca
    22861 ggacaaggca gggtaatacg catcatcact ggcaaataat tgataaaaca tatcattgcc
    22921 ggaatcgtcg tattctttac gagctaagaa ttcgcgtaat aaaggcagat gatcatcttc
    22981 gggagactgc caatattgcc gaccaaaggt ataaccggca aagtattgcc gccagttact
    23041 ataataacgt agagcccgta gatgaatacg cgactggatc catagacttt cttcttcact
    23101 caaccagtta ttaagtacgc ccattcgact taaaaaaccc atccggacat aatcccatgc
    23161 tttaattcct ccctcaccac agcattcggc tgtgctggcg acaaattgcg cataagcctg
    23221 accttgctca tttagttcag caagatagtc acgccattca catggcgaat aacgaaacca
    23281 gcggtgataa aaccctgcca accgagcggc atgaccgtta tcgatcattc ggtgaattgt
    23341 gcgcagcagg tcatcacggt catcaattcc ccatgaggaa gaaaggtcaa aaaagcatgt
    23401 acctaaatac attttggggg atgaatgcga aggtaaacag cattcattgc gtatttccat
    23461 cggagtggaa agtgcaaaca atattttttg cgaccccatg tccatataat ttatccttaa
    23521 acattcattt aatgaatgta aactacattt cgagactcag ttatttatac ttttctgacg
    23581 caacataata cgcataaaat aaggcttacg caaaaatgga acacataaaa atataatatg
    23641 ttttatctac aaccataagg aaatgataat ggtttttacg tcaagttgct gcgataattt
    23701 atcaatagat gagattatcg aacgtgctga aaaaggcgat tgcgaggctc aatatattgt
    23761 tgggttttat tataatcgcg atagcgcaat tgactctccg gacgacgaaa aggcctttta
    23821 ctggctgaag ctggccgctg agcaaggtca ttgtgaagca cagtattcct tagggcaaaa
    23881 gtataccgag gataaaagcc gtcataaaga taatgagcaa gccatcttct ggctgaaaaa
    23941 agctgcccta caaggccata ctttcgcttc caacgccctt ggctggacac tggatcgtgg
    24001 agaagccccc aattataaag aagcggttgt ctggtatcag atagccgcgg agagcggaat
    24061 gtcttatgcg caaaataatc ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga
    24121 ctatgcgctg gcatttttct ggtacaaaca agctgcatta caaggccata gtgacgcgca
    24181 aaacaatctg gccgatcttt atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc
    24241 cgcattctgg tatttgaaaa gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc
    24301 atgggattat aacgctggcg aaggggtgga ccaggactat aagcaagcga tgtactggta
    24361 tctgaaggct gccgctcagg aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa
    24421 acacggacaa ggcgttgaga aagattatca ggctgccttt gaatggttta cgaaagccgc
    24481 tgaatgcaat gacgccactg cctggtataa cctggccatt atgtatcact acggagaagg
    24541 aagacctgtc gatctccgac aggctctcga cctgtatcgt aaagttcagt catccggaac
    24601 cagagatgtc agtcaggaaa ttcgtgagac tgaagattta ctgtagagat aaaaacaagg
    24661 gccggggaat ttcattcggc ccatattctc tttattgctg tgcgaaatca gactattacg
    24721 gctcaaacgg gcgtctctgg aactggtcat gaccacattt cgggcatagc gtcagcactt
    24781 ccggtgtgta gatcgggaga tggaagtgac atttctcgca gaccagattt cccagcccga
    24841 ccacttctcc gctgtgataa accccatgat gattgaggtc ctggaaaact tcgcgccatt
    24901 caagctgcgt tttatcggtg atatctgcca gctcctgcca taagctttct ttaatcaccc
    24961 gcataaagac gctgtcagat tcttctttca ggctctcttc atagctcatg gcgaactctt
    25021 ccaggtcacg tcttacagct cgcgtcagct catcgacctc ggttcgcgtt aactcccctg
    25081 tttttattac gcgctcgcgc gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc
    25141 gcaggcgttc gctcagtgac gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg
    25201 tttcatctcc cgggtcgtta actgactata ataatagacg ttctttgttc cttgctttgc
    25261 taatacgccc acaaactctg taaattaatg tatgaataat ccgggcatca tttcaacgtc
    25321 gagtgcgcga aaggctgttt tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa
    25381 ccacctcaac gctacatttg tagccgtatt gaaaacagga ccactggctg ccatgcaaga
    25441 gcaataccgc ccggaagaga tagaatccaa agtacagctt cattgggatg agaagcgcac
    25501 atttgaagta accgaagacg agagcaaaga gaagtattac tgcctgtcta tgcttcccta
    25561 tccttctggt cgactacaca tgggccacgt acgtaactac accatcggtg acgtgatcgc
    25621 ccgctaccag cgtatgctgg gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg
    25681 tctgcctgcg gaaggcgcgg cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga
    25741 caacatcgcg tatatgaaaa accagctcaa aatgctgggc tttggttatg actggagccg
    25801 cgagctggca acctgtacgc cggaatacta ccgttgggaa cagaaattct tcaccgagct
    25861 gtataaaaaa ggcctggtat ataagaagac ttctgcggtc aactggtgcc cgaacgacca
    25921 gaccgtactg gcgaacgaac aagttatcga cggctgctgc tggcgctgcg ataccaaagt
    25981 tgaacgtaaa gagatcccgc agtggtttat caaaatcact gcttacgctg acgagctgct
    26041 caacgatctg gataaactgg atcactggcc agacaccgtt aaaaccatgc agcgtaactg
    26101 gatcggtcgt tccgaaggcg tggagatcac cttcaacgtt aacgactatg acaacatgct
    26161 gaccgtttac actacccgcc cggacacctt tatgggttgt acctacctgg cggtagctgc
    26221 gggtcatccg ctggcgcaga aagcggcgga aaataatcct gaactggcgg cctttattga
    26281 cgaatgccgt aacaccaaag ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt
    26341 cgatactggc tttaaagctg ttcacccatt aacgggcgaa gaaattcccg tttgggcagc
    26401 aaacttcgta ttgatggagt acggcacggg cgcagttatg gcggttccgg gtcacgacca
    26461 gcgcgactac gagtttgcct ctaaatacgg cctgaacatc aaaccagtta tcctggctgc
    26521 tgacggctct gagccagatc tttctcagca agccctgact gaaaaaggcg tgctgttcaa
    26581 ctctggcgaa ttcaacggtc ttgaccatga agcggccttc aacgccatcg ccgataaact
    26641 gactgcgatg ggcgttggcg agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc
    26701 tcgtcagcgt tactggggcg cgccgattcc gatggtgacg ctggaagacg gtaccgtaat
    26761 gccgaccccg gacgaccagc tgccggtgat cctgccggaa gatgtggtga tggacggcat
    26821 taccagcccg attaaagcag atccggagtg ggcaaaaact accgttaacg gtatgccagc
    26881 gctgcgtgaa accgacactt tcgacacctt tatggagtcc tcctggtact atgcgcgcta
    26941 cacttgccca gagtacaaag aaggtatgct ggattccgaa gcggctaact actggctgcc
    27001 ggtggatatc tacattggtg gtatcgaaca cgccattatg cacctgctct acttccgctt
    27061 cttccacaaa ctgatgcgtg atgcaggcat ggtgaactct gacgaaccag cgaaacagtt
    27121 gctgtgtcag ggtatggtgc tggcagatgc cttctactat gttggcgaaa acggcgaacg
    27181 taactgggtt tccccggttg atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa
    27241 agcgaaagat gcggcaggcc atgaactggt ttataccggc atgagcaaaa tgtccaagtc
    27301 gaagaacaac ggtatcgacc cgcaggtgat ggttgaacgt tacggcgcgg acaccgttcg
    27361 tctgtttatg atgtttgctt ctccggctga tatgactctc gaatggcagg aatccggtgt
    27421 ggaaggggct aaccgcttcc tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa
    27481 aggtgatgtt gcggcactga acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg
    27541 cgatgtgcat aaaacgatcg ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa
    27601 caccgcaatt gcggcgatta tggagctgat gaacaaactg gcgaaagcac caaccgatgg
    27661 cgagcaggat cgcgctctga tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc
    27721 gttcaccccg cacatctgct tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga
    27781 caacgcgccg tggccggttg ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt
    27841 ggtgcaggtt aacggtaaag tccgtgccaa aatcaccgtt ctggtggacg caacggaaga
    27901 acaggttcgc gaacgtgctg gccaggaaca tctggtagca aaatatcttg atggcgttac
    27961 cgtgcgtaaa gtgatttacg taccaggtaa actcctcaat ctggtcgttg gctaagcgcg
    28021 ggaggaagcg tgcgatatct ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc
    28081 gggtgtggct ggcatctgcg tgataccacg caggttcctt ccactatgaa ggtcatgatc
    28141 ctggactcag gcgatccgaa cgggccatta agccgtgcag tgcgtaacca gttacgtctg
    28201 aatggtgtcg agttgcttga taaagaaacc acgcgtaagg acgttccatc cctgcgtttg
    28261 ggtaaagtga gcatcgcgaa agataccgca tcggtattcc gtaacggtca aacagcagag
    28321 tatcagatga tcatgacggt gaatgcgacc gtgttgatcc ccggccgtga tatctacccg
    28381 attagcgcca aagtcttccg ttcgttcttt gataacccgc aaatggcgtt agcgaaagat
    28441 aacgaacaag acatgatcgt aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt
    28501 aagctgccaa gcatccgtgc tgcggatatt cgttccgacg aagaacagac gtcgaccaca
    28561 acggatactc cggcaacgcc tgcacgcgtc tccaccacgc tgggtaactg atgattcggt
    28621 tgtacccgga acaactccgc gcgcagctca atgaagggct gcgcgcggcg tatcttttac
    28681 ttggtaacga tcctctgtta ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg
    28741 cacaaggatt cgaagaacac cacacttttt ccattgatcc caacactgac tggaatgcga
    28801 tcttttcgtt atgccaggct atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt
    28861 taccagaaaa cggaccgaat gcggcgatca atgaacaact tctcacactc accggacttc
    28921 tgcatgacga cctgctgttg atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg
    28981 ccgcctggtt tactgctctt gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc
    29041 aggctcagct tccccgctgg gttactgcgc gcgcaaaaca gctcaactta gaactggatg
    29101 acgcggcaaa tcaggtgctc tgctactgtt atgaaggtaa cctgctggcg ctggctcagg
    29161 cactggagcg tttatcgctg ctctggccag acggcaaatt gacattaccg cgcgttgaac
    29221 aggcggtgaa tgatgccgcg catttcaccc cttttcattg ggttgatgct ttgttgatgg
    29281 gaaaaagtaa gcgcgcattg catattcttc agcaactgcg tctggaaggc agcgaaccgg
    29341 ttattttgtt gcgcacatta caacgtgaac tgttgttact ggttaacctg aaacgccagt
    29401 ctgcccatac gccactgcgt gcgttgtttg ataagcatcg ggtatggcag aaccgccggg
    29461 gcatgatggg cgaggcgtta aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac
    29521 tcctgacacg aacggaactc accctcaaac aagattacgg tcagtcagtg tgggcagagc
    29581 tgaaagggtt atctcttctg ttgtgccata aacccctggc ggacgtattt atcgacggtt
    29641 gatatgaaat ctttacaggc tctgtttggc ggcacctttg atccggtgca ctatggtcat
    29701 ctaaaacccg tggaaacgct ggcgaatttg attggtctga cgcgggtcac aatcatccct
    29761 aataatgttc ctccgcatcg tccccagccg gaagcgaaca gcgtgcagcg taaacacatg
    29821 cttgaactgg cgattgccga caagccatta tttactcttg atgaacgcga gctaaagcgc
    29881 aatgccccct cttacactgc gcaaacactg aaagagtggc ggcaggaaca aggaccggac
    29941 gtgccgctgg cgtttattat tggtcaggat tcactgctga cctttccgac ctggtacgaa
    30001 tacgaaacga tactcgacaa tgcacatttg atcgtctgtc ggcgtccagg ttacccactt
    30061 gaaatggcgc aaccgcaata ccagcaatgg ctggaagatc atttgacaca taacccggaa
    30121 gatcttcacc ttcagcctgc cggtaaaatt tatctggctg aaacgccgtg gtttaacatc
    30181 tcggcgacca tcatccgcga acgtttgcaa aacggtgaat cgtgtgagga tttattgccg
    30241 gaaccggtac tgacttacat taaccaacaa ggcttgtatc gctgatacct gtcgttacgg
    30301 agggcatgat gcgactgtgg ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc
    30361 tttacagcgg tcatgcgccc acccccctga ccgcgcgcgg tattgagcaa gcgcaaaatc
    30421 tgcatacgct gctacatggt gtttcctttg atctggtttt atgcagtgaa ctggaacggg
    30481 cacagcatac cgcacgactg gttctcagtg accgccagct ccccgtgcaa atcatccctg
    30541 aactcaacga aatgtttttt ggcgactggg agatgcgaca tcatcgcgac ctcatgcaag
    30601 aagatgccga aaactatagc gcgtggtgca atgactggca gcatgcaatc cccacgaacg
    30661 gtgaaggatt tcaggcattt tcgcaacgtg tggaacgctt tatcgcaagg cttagtgaat
    30721 ttcagcacta tcagaatatt ttagtcgtca gccatcaggg tgtactgagt ctgttaatcg
    30781 cccgtttaat tggcatgcct gccgaggcta tgtggcattt tcgcgttgac taggggtgtt
    30841 ggagcgccat tgatatcaac caaaaattcg cgacgctacg cgtcctcaat agccgtgcca
    30901 tcggggtcga gaatgcatga cttttctgtt tttttacggg taagccgcaa cgaccattga
    30961 cagacccggg caggctgata ttctccgcag ccagactttt ttcgccagac acgactttgt
    31021 agaaattgtt ttacaaaaat ggcgatgcaa tctgcggcgc ggggtgggat gatagcccac
    31081 tttcgaaagc cgattcggcg acaattgtcc cgaaatcgcc tctggttcag gtatactgac
    31141 agaccatttt tatctatttg attcacccag ggggaaaact tgcagggtaa agcactccag
    31201 gattttgtta tcgacaaaat tgatgacctc aaaggtcagg acatcatcgc cttagacgtt
    31261 caaggcaaat ccagcatcac cgactgcatg atcatctgta cgggtacgtc cagccgtcat
    31321 gttatgtcca ttgctgacca cgttgtgcag gagtctcgcg cagcgggcct gttaccgctc
    31381 ggcgtagaag gtgaaaacag cgccgactgg attgtcgtgg atttgggcga tgtgattgtc
    31441 catgtcatgc aggaagagag ccgtcgcctg tatgaactgg aaaaactctg gagttaatgc
    31501 gtgaagctgc aacttgtcgc cgtgggaacg aaaatgccgg actgggtaca aaccggtttt
    31561 accgagtacc tgcgtcgttt tccgaaagat atgcccttcg agctgattga aattccggcc
    31621 ggaaaacgcg gcaagaatgc ggacatcaag cgcatactcg acaaagaggg tgagcagatg
    31681 ttggcggccg caggcaaaaa ccgcattgtc accctcgata ttccaggcaa gccctgggat
    31741 acgccgcagt tagccgctga gctggaacgc tggaagctgg atggtcgcga cgtcagtcta
    31801 ctgattggcg ggcctgaagg gttgtcgcct gcctgtaaag cggcggcgga gcagagctgg
    31861 tcgctgtcgg cgcttaccct cccccatccg ctggttcgcg tgctggtcgc agagagtctg
    31921 taccgggcgt ggagcatcac caccaaccat ccttatcacc gtgagtgata agggagcttt
    31981 gagtagaaaa cgcagcggat gaaactacag aactcttttc gcgactatac ggcagagtcc
    32041 gcgctgtttg tgcgccgggc gctggtcgcc tttttgggga ttttgctgct gaccggcgtg
    32101 cttatcgcca acctgtataa tctgcaaatt gttcgcttta ccgactacca gactcgctct
    32161 aatgaaaacc gcattaagct ggtgcctatc gcgcccagcc gcggcattat ctacgaccgt
    32221 aatggtatcc ctctggccct caaccgcact atctaccaga tagaaatgat gccagagaaa
    32281 gtcgataacg tgcagcaaac gctggacgct ttgcgcagcg tggttgatct gaccgatgac
    32341 gatattgctg cattccgaaa agagcgcgca cgttcacacc gtttcacctc tattccggtg
    32401 aaaactaacc tgaccgaagt acaagtagct cgctttgccg tcaatcagta ccgttttccg
    32461 ggtgtcgaag ttaaaggcta taaacgtcgt tactatcctt acggttcggc gttgacccac
    32521 gtcatcggct atgtgtcgaa aatcaacgat aaagacgtcg aacgcctgaa taatgacggc
    32581 aaactggcca actatgcggc aacgcatgat atcggtaagc tgggcattga gcgttactat
    32641 gaagacgtgc tgcacggtca gaccggttat gaagaggttg aagttaacaa ccgtgggcgt
    32701 gttatccgcc agttaaaaga agtaccaccg caagccggac acgatattta cctgacgctg
    32761 gatctcaaac tccagcaata tattgaaacg ctgctggcgg gtagccgcgc agctgtggtt
    32821 gtcaccgatc cgcgtacagg tggggtgctg gcgctggttt ccacgcctag ttatgaccca
    32881 aacctgtttg ttgacggtat ctccagcaaa gattattccg ccttgttgaa cgccccgaat
    32941 acaccgctgg tgaaccgcgc cacacagggg gtttatcctc ccgcgtctac agttaaaccc
    33001 tatgtggcgg tttcggcatt gagcgccggg gtgatcacgc gcaatacgac gctgtttgac
    33061 ccaggctggt ggcaactgcc aggttcggaa aaacgttatc gtgactggaa aaaatggggc
    33121 cacgggcgtc tgaatgtcac aagatcgctg gaagaatctg cggatacctt cttctatcag
    33181 gtggcctacg atatggggat cgatcgcctc tccgaatgga tgggtaaatt cggttatggt
    33241 cattacaccg gtatcgacct ggcggaagaa cgttccggca acatgcctac ccgcgaatgg
    33301 aaacagaaac gctttaaaaa accgtggtat cagggtgaca ccattccggt tggtatcggt
    33361 cagggttact ggacagcgac cccaatccag atgagtaagg cactgatgat cctgattaat
    33421 gacggtatcg tgaaggttcc tcatttgctg atgagcaccg ccgaagacgg caaacaggtg
    33481 ccatgggtac agccgcatga accgcccgtc ggcgatattc attccggtta ctgggagctg
    33541 gcgaaagacg gtatgtacgg tgttgctaac cgccctaacg gtacggcgca taaatacttt
    33601 gccagcgcac cgtacaaaat tgcggcgaaa tccggtaccg ctcaggtctt cggtctgaaa
    33661 gcgaacgaaa cctataatgc gcacaaaatt gccgagcgtt tacgtgacca caaactgatg
    33721 accgcctttg cgccatacaa caatccgcaa gtggctgtcg ccatgattct ggagaacggt
    33781 ggtgcgggtc cggcggttgg tacactgatg cgccagatcc tcgaccacat tatgctgggt
    33841 gataacaaca ccgatctgcc tgcggaaaat ccagcggttg ccgcagcgga ggaccattaa
    33901 tcatgacgga taatccgaat aaaaaaacat tctgggataa agtccatctc gatcccacaa
    33961 tgctgctgat cttactggca ttgctggttt acagcgccct ggttatctgg agcgccagcg
    34021 gtcaggatat tggcatgatg gagcgtaaaa tcggccaaat cgcgatgggt ctagtcatca
    34081 tggtggtgat ggcgcaaatt cctccacgcg tttatgaagg ctgggccccc tatctctata
    34141 tcatctgtat tattttgctg gtggcggtag atgctttcgg tgccatctct aaaggtgctc
    34201 aacgctggct ggacctcggt attgttcgtt ttcagccgtc ggaaattgcc aaaatagccg
    34261 taccactgat ggttgcgcgc tttatcaacc gcgacgtttg cccgccatcg ttgaagaaca
    34321 ctggcatcgc gctggtgctg atatttatgc ccacgctgct ggtggctgca cagcctgacc
    34381 tgggaacatc aatcctcgtt gcgctttccg gtctgtttgt actgttcctc tctggcctta
    34441 gctggcgtct gattggcgtc gcagtagtgc tggtggcggc gttcattccg attctgtggt
    34501 tcttcctgat gcatgattac cagcgccagc gcgtaatgat gctcctagac ccggaatcag
    34561 acccactcgg cgcgggctat cacattattc agtctaaaat tgctattggc tccggcggat
    34621 tacgcggcaa aggctggctg cacggcactc agtcacagct tgaatttctc cccgaacgcc
    34681 ataccgactt tatcttcgcg gtactggcgg aagagctggg attagtgggc attctgattc
    34741 tgctcgctct ctacattctg ctgatcatgc gcgggctgtg gatagccgcc agagcgcaaa
    34801 ccacctttgg tcgcgtcatg gctggtggct taatgctgat attattcgtt tatgtcttcg
    34861 taaatattgg tatggtaagc ggtattctgc cggttgtagg ggttccgctc ccactggtca
    34921 gttatggagg atcggcgcta attgtgctga tggctgggtt cgggattgta atgtcaatcc
    34981 acacccacag gaaaatgttg tcgaaaagcg tgtaagaggt gcgcaatgcg taagcagtgg
    35041 ctcgggatct gcatcgcggc aggaatgctc gcggcatgta caagcgatga tggtcagcaa
    35101 cagacggtaa gtgtaccgca gcctgcggta tgtaacggcc ccatagttga aattagcggg
    35161 gcggacccgc gtttcgaacc actgaacgcg acggcaaatc aggattacca gcgcgacggt
    35221 aaaagctaca aaatcgtgca ggatccgtct cgatttagcc aggcgggact ggcggcaatc
    35281 tatgatgccg aacccggcag taacctgacg gcctctggcg aagctttcga tccgacacag
    35341 ctgacggcgg cccatccaac gcttccgatc cccagctacg ccagaatcac taacctggct
    35401 aacgggcgaa tgatcgtggt gcgcattaat gatcgcggcc cttacggcaa cgacagcgtt
    35461 atttcacttt ctcgcgcggc agctgaccgt cttaacacgt caaacaacac caaagttcgt
    35521 atcgatccga ttattgtcgc ccaggatggt tcgctttctg gtcctggtat ggcttgtacc
    35581 acagtcgcca aacagactta cgccctgcca gcacctcccg atttaagcgg tggcgcggga
    35641 acaagttcag tgtctggccc gcagggtgac attcttccgg tcagtaattc gacgctaaaa
    35701 agcgaagatc cgaccggcgc gccggtaacc agtagcggtt tcctcggcgc tccaacgacc
    35761 ttagcgcctg gtgtactgga aggcagcgaa ccgacgcctg cgccacagcc cgttgttaca
    35821 gctccgtcga caacgcctgc aacctcgcct gcaatggtga caccgcaagc cgcctcgcaa
    35881 agcgccagcg gcaactttat ggtgcaagtc ggggccgtaa gcgatcaggc tcgtgcgcaa
    35941 cagtaccaac agcaactggg acagaagttc ggcgtccccg gtcgcgtaac tcaaaatggc
    36001 gcggtctggc ggatccagct tggcccattc gccagcaaag ccgaagccag taccttgcag
    36061 caacgtttgc aaaccgaagc ccaattacag tcatttatta ctaccgcgca gtagcgtaaa
    36121 gcaggcatct gatgtgtcaa tttctgtaaa tgacgttaac aaagtcacgc ggaaagtcag
    36181 atgcctgccg gtagtgcatt tgctatagta gggcactttt ttaattccat cacggatgtc
    36241 gtagttcaga ccatgaatac cattttttcc gctcgtatca tgaagcgcct ggcgctcacc
    36301 acggctcttt gcacagcctt tatctctgct gcacatgccg atgacctgaa tatcaaaact
    36361 atgatcccgg gtgtaccgca gatcgatgcg gagtcctaca tcctgattga ctataactcc
    36421 ggcaaagtgc tcgccgaaca gaacgcagat gtccgccgcg atcctgccag cctgaccaaa
    36481 atgatgacca gttacgttat cggccaggca atgaaagccg gtaaatttaa agaaactgat
    36541 ttagtcacta tcggcaacga cgcatgggcc accggtaacc cggtgtttaa aggttcttcg
    36601 ctgatgttcc tcaaaccggg catgcaggtt ccggtttctc agctgatccg cggtattaac
    36661 ctgcaatcgg gtaacgatgc ttgtgtcgcc atggccgatt ttgccgctgg tagccaggac
    36721 gcttttgttg gcttgatgaa cagctacgtt aacgcactgg gcctgaaaaa tacccacttc
    36781 cagacggtac atggtctgga tgctgatggt cagtacagct ccgcgcgaga tatggcgctg
    36841 atcggccagg cattgatccg tgacgtaccg aatgaatact cgatctataa agaaaaagaa
    36901 tttacgttta acggtattcg ccagctgaac cgtaacggcc tgttatggga taacagcctg
    36961 aatgtcgacg gcatcaaaac cggacacact gacaaagcag gttacaacct tgttgcttct
    37021 gcgactgaag gccagatgcg cttgatttct gcggtgatgg gcggacgtac ttttaaaggc
    37081 cgtgaagccg aaagtaaaaa actgctaacc tggggcttcc gtttcttcga aaccgttaac
    37141 ccactgaaag taggtaaaga gttcgcctct gaaccggttt ggtttggtga ttctgatcgc
    37201 gcttcgttag gggttgataa agacgtgtac ctgaccattc cacgtggtcg catgaaagat
    37261 ctgaaagcca gctatgtgct gaacagcagt gaattgcatg cgccgctgca aaagaatcag
    37321 gtcgtcggaa ctatcaactt ccagcttgat ggcaaaacga tcgagcaacg cccgctggtt
    37381 gtgttgcaag aaatcccgga aggtaacttc ttcggcaaaa tcattgatta cattaaatta
    37441 atgttccatc actggtttgg ttaaaaattg aacacttgaa agtgtaattt ccgtccccat
    37501 atactaagca tcagtaaaaa actcccgcct tctggcggga gttgctattt aattacgtta
    37561 cgccggagct gacatgaaaa ccaaacttaa cgaactgctt gaattcccta ctccttttac
    37621 ttacaaagtt atggggcagg cgttacctga gctggttgat caggtggttg aagtggtaca
    37681 gcgccatgcg ccaggtgact acaccccaac ggtaaaacca agcagcaaag gcaactacca
    37741 ctcggtatct atcactatca acgccactca tatcgagcag gttgaaacac tgtatgaaga
    37801 actaggcaaa atcgatattg tccgcatggt tctgtaactc gcttctccgt tacccggccc
    37861 ctggtcgggt aactcccctc tcctcgctgt gatatacttt tcccccactt ttactcattc
    37921 tccacggaga tgcagttttg tatcaggata aaattcttgt ccgccagctc ggtcttcagc
    37981 cttacgagcc aatctcccag gctatgcatg aattcaccga tacccgcgat gatagtaccc
    38041 ttgatgaaat ctggctggtc gagcactatc cggtattcac ccaaggtcag gcaggaaaag
    38101 cggagcacat tttaatgccg ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg
    38161 tgacttatca cgggccgggg caacaggtga tgtatgtgtt gcttaacctg aaacgccgta
    38221 aactcggtgt gcgtgaactg gtgaccttgc ttgagcaaac agtggtgaat accctggctg
    38281 aactgggtat agaagcgcat cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga
    38341 aaatttgctc actgggttta cgtattcgac gcggttgttc attccacggt ctggcattaa
    38401 acgtcaatat ggatctttca ccatttttac gtattaatcc ttgtgggtat gccggaatgg
    38461 aaatggctaa aatatcacaa tggaaacccg aagcgacgac taataatatt gcaccacgtt
    38521 tactggaaaa tattttagcg ctactaaaca atcctgactt cgaatatatt accgcttaat
    38581 tgcatacctc aatggcccaa tttacactgg gtcattactc ataatacctg ctaatgcttt
    38641 acatctcctt cgcgtttgta tactttcttt atcacttgca gaaatgcggt gctacgcctt
    38701 gctccgtaac tcaccccatt gattaaccta ttgaaatagt tgattatggt gagcaaaagc
    38761 gttccgttcc aattagacat aagattattg taattttttt aaatattatt tttccatgaa
    38821 taaaattgga gtaagtgcgt ggatagtaat aatcaaattg aaccctgttt aagcagaaag
    38881 tcgtcagaag gtaaaccaca aatatttaca accttacgaa atattgatct taaccttctg
    38941 actatttttg aagctgtata tgtacataaa gggatcgtta atgcagcgaa agtgcttaat
    39001 ctgaccccct cggcaatcag tcagtctatt cagaaactgc gcgttatatt ccctgaccca
    39061 ttgtttattc gcaaaggcca gggtgtcact cctaccgcat ttgcgatgca tctacatgag
    39121 tatatcagtc agggccttga gtccattctt ggcgcgctgg atatcgaagg aagctatgat
    39181 aagcaacgaa cgataactat tgcgaccact ccctcggtcg gagccctggt ccttcctgtc
    39241 atctatcggg cgattaaaac tcactatccg cagcttttac tgcgcaaccc gcccatcagc
    39301 gacgcggaaa accaactcag tcagtttcaa accgatctca tcatcgataa catgttttgc
    39361 accaatcgta cggtgcaaca tcatgttctg ttcaccgaca atatggtgtt aatttgccgc
    39421 gagggaaatc cactactctc tttagaagat gacagagaga ctatcgacaa cgctgcgcat
    39481 gtactcctgt taccggaagg gcaaaatttc agcggtctgc ggcagagagt tcaagagatg
    39541 tttccggacc ggcaaatcaa tttcaccagc tacaacattt tgacaatcgc tgcactggtt
    39601 gccaacagtg acatgttagc gattattccc agccgttttt ataacctgtt tagtcgttgc
    39661 tggccgctgg aaaaactgcc ttttccgtcc ttaaatgagg agcaaataga tttctctatc
    39721 cactacaaca aattcagcct gcgtgatccc atcctgcacg gggtaataga tgttatccgt
    39781 aatgcatttt gaaaaagtat tgttacagcc agatcacagc gcagaacgat aaaatggcac
    39841 aatccaacaa caattttaca ttttcgcgac cgctttggct gctttcaggt ccgtttcaat
    39901 gatatactgc cagtcgttaa ttcaaaaata gttgataatt acaacaatct attgaattga
    39961 aacgctttcc ttcgtaattc gcaactggaa cacgcacgct atgagtaaac ccattgtgat
    40021 ggaacgcggt gttaaatacc gcgatgccga taagatggcc cttatcccgg ttaaaaacgt
    40081 ggcaacagag cgcgaagccc tgctgcgcaa gccggaatgg atgaaaatca agcttccagc
    40141 ggactctaca cgtatccagg gcatcaaagc cgcaatgcgc aaaaatggcc tgcattctgt
    40201 ctgcgaggaa gcctcctgcc ctaacctggc ggaatgcttc aaccacggca cagcaacgtt
    40261 tatgatcctc ggcgctattt gtacccgccg ttgtccgttc tgtgatgttg cccacggtcg
    40321 cccggtagct cctgatgcca atgaaccagt gaaactggcg cagaccattg ccgatatggc
    40381 gctgcgttat gtggttatca cctccgttga ccgtgatgac ctgcgcgatg gcggtgccca
    40441 gcactttgcg gattgcatta ctgccattcg ggaaaaaagc ccgcaaatca aaattgaaac
    40501 tctggtgccg gatttccgtg gtcgtatgga tcgtgctctg gatattctga ctgcaacgcc
    40561 accagatgtg ttcaaccata acctggaaaa cgtaccgcgt atttaccgtc aggtacggcc
    40621 tggtgcagat tacaactggt cgctgaagct gctggaacgc tttaaagaag cgcatccgga
    40681 aatcccgacc aagtctggtc tgatggtggg actgggtgaa accaatgaag aaattattga
    40741 ggtaatgcgc gacctgcgcc gtcatggtgt gacgatgtta acgctggggc aatatttgca
    40801 gccaagccgc catcacctgc cggttcaacg ttacgttagc ccggatgagt tcgacgaaat
    40861 gaaagccgaa gcgctggcga tgggctttac ccatgctgca tgcggtccgt ttgtccgctc
    40921 ttcttaccac gccgatttgc aggcgaaagg gatggaagtt aagtaatcct gtaataatta
    40981 cttacatctg tctgataaaa aacccgcttt ttg
//
LOCUS       DAJTVK010000021        40936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000021 DAJTVK010000000
VERSION     DAJTVK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.021"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..789
                     /locus_tag="OO372_001213"
     CDS             <1..789
                     /locus_tag="OO372_001213"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCR6340767.1"
                     /translation="LITRITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNL
                     LNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTY
                     PLSPVNSMAPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMN
                     PISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDL
                     TNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            791..1027
                     /locus_tag="OO372_001214"
     CDS             791..1027
                     /locus_tag="OO372_001214"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340768.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(1200..1875)
                     /locus_tag="OO372_001215"
                     /pseudo
     CDS             complement(1200..1875)
                     /locus_tag="OO372_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2020..2628)
                     /locus_tag="OO372_001216"
     CDS             complement(2020..2628)
                     /locus_tag="OO372_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6340769.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2812..3129)
                     /gene="metJ"
                     /locus_tag="OO372_001217"
     CDS             complement(2812..3129)
                     /gene="metJ"
                     /locus_tag="OO372_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6340770.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            3406..4566
                     /gene="metB"
                     /locus_tag="OO372_001218"
     CDS             3406..4566
                     /gene="metB"
                     /locus_tag="OO372_001218"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6340771.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4569..7001
                     /gene="metL"
                     /locus_tag="OO372_001219"
     CDS             4569..7001
                     /gene="metL"
                     /locus_tag="OO372_001219"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6340772.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            7350..8240
                     /gene="metF"
                     /locus_tag="OO372_001220"
     CDS             7350..8240
                     /gene="metF"
                     /locus_tag="OO372_001220"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6340773.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8569..10749
                     /gene="katG"
                     /locus_tag="OO372_001221"
     CDS             8569..10749
                     /gene="katG"
                     /locus_tag="OO372_001221"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6340774.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10843..11748
                     /gene="yijE"
                     /locus_tag="OO372_001222"
     CDS             10843..11748
                     /gene="yijE"
                     /locus_tag="OO372_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6340775.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(11775..12392)
                     /locus_tag="OO372_001223"
     CDS             complement(11775..12392)
                     /locus_tag="OO372_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR6340776.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12666..13769)
                     /gene="gldA"
                     /locus_tag="OO372_001224"
     CDS             complement(12666..13769)
                     /gene="gldA"
                     /locus_tag="OO372_001224"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR6340777.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13780..14442)
                     /gene="fsa"
                     /locus_tag="OO372_001225"
     CDS             complement(13780..14442)
                     /gene="fsa"
                     /locus_tag="OO372_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6340778.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14454..16955)
                     /gene="ptsP"
                     /locus_tag="OO372_001226"
     CDS             complement(14454..16955)
                     /gene="ptsP"
                     /locus_tag="OO372_001226"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6340779.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            17264..18343
                     /locus_tag="OO372_001227"
     CDS             17264..18343
                     /locus_tag="OO372_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6340780.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            18358..18678
                     /locus_tag="OO372_001228"
                     /pseudo
     CDS             18358..18678
                     /locus_tag="OO372_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            18729..21025
                     /locus_tag="OO372_001229"
                     /pseudo
     CDS             18729..21025
                     /locus_tag="OO372_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            20991..21869
                     /locus_tag="OO372_001230"
     CDS             20991..21869
                     /locus_tag="OO372_001230"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6340781.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21871..22212
                     /locus_tag="OO372_001231"
     CDS             21871..22212
                     /locus_tag="OO372_001231"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6340782.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(22199..23050)
                     /locus_tag="OO372_001232"
     CDS             complement(22199..23050)
                     /locus_tag="OO372_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6340783.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(23265..24998)
                     /locus_tag="OO372_001233"
     CDS             complement(23265..24998)
                     /locus_tag="OO372_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6340784.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(25181..27832)
                     /gene="ppc"
                     /locus_tag="OO372_001234"
     CDS             complement(25181..27832)
                     /gene="ppc"
                     /locus_tag="OO372_001234"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6340785.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(28184..29335)
                     /gene="argE"
                     /locus_tag="OO372_001235"
     CDS             complement(28184..29335)
                     /gene="argE"
                     /locus_tag="OO372_001235"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6340786.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            29489..30493
                     /gene="argC"
                     /locus_tag="OO372_001236"
     CDS             29489..30493
                     /gene="argC"
                     /locus_tag="OO372_001236"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6340787.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            30501..31277
                     /gene="argB"
                     /locus_tag="OO372_001237"
     CDS             30501..31277
                     /gene="argB"
                     /locus_tag="OO372_001237"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6340788.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            31338..32711
                     /gene="argH"
                     /locus_tag="OO372_001238"
     CDS             31338..32711
                     /gene="argH"
                     /locus_tag="OO372_001238"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6340789.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32978..33895
                     /gene="oxyR"
                     /locus_tag="OO372_001239"
     CDS             32978..33895
                     /gene="oxyR"
                     /locus_tag="OO372_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6340790.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(33878..35278)
                     /gene="sthA"
                     /locus_tag="OO372_001240"
     CDS             complement(33878..35278)
                     /gene="sthA"
                     /locus_tag="OO372_001240"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6340791.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35599..36249
                     /gene="fabR"
                     /locus_tag="OO372_001241"
     CDS             35599..36249
                     /gene="fabR"
                     /locus_tag="OO372_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6340792.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            36249..36608
                     /locus_tag="OO372_001242"
     CDS             36249..36608
                     /locus_tag="OO372_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6340793.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36648..37748)
                     /gene="trmA"
                     /locus_tag="OO372_001243"
     CDS             complement(36648..37748)
                     /gene="trmA"
                     /locus_tag="OO372_001243"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6340794.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37862..38052
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            38117..39961
                     /gene="btuB"
                     /locus_tag="OO372_001244"
     CDS             38117..39961
                     /gene="btuB"
                     /locus_tag="OO372_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6340795.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39906..40763
                     /gene="murI"
                     /locus_tag="OO372_001245"
     CDS             39906..40763
                     /gene="murI"
                     /locus_tag="OO372_001245"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6340796.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9460 a        10202 c        11241 g        10033 t
ORIGIN      
        1 ctcatcacgc gcatcaccac gccggatggc agggcatcgg cgttttacta taaccaccac
       61 agccagttaa cgtcagccac cgggcctgac gggctggaaa tacgccggga atatgatgaa
      121 tggggcaacc tgctgaatga agagaacccg catcagctgc agcagcttat tcgtttgccg
      181 gggcagcagt atgatgagga gtccggcctg tattataacc gccaccgcta ttatgacccg
      241 ctgcaggggc ggtatatcac tcaggatccg attgggctga aggggggatg gaacctgtat
      301 acatatccgc tgagcccggt gaatagcatg gctccattag gattatatga atttaaatca
      361 aaaaatattg atgatattgg aatatttgca ttggcaatgt gtaatggaga atcaattaac
      421 gagaataaag aatatggtgg actaatatgt aagaagcaag gtgaatattt ccccatgaat
      481 ccgataagtt caaatgataa tgatagtgta gacttgcgaa atataaaatg ccctgaaggt
      541 tcagagagag taggcgatta tcacactcac ggtttttact ctgacgataa aggaaataaa
      601 gtaacaaaag aaaatgatgt ttatgatagt ctaaattttt caagcaaaga tttaacgaat
      661 tcttatatga atggaatggg aaaaaaagaa tacagtagtt acttgggaac accaaataac
      721 acctatctaa aatataatcc caaagctaaa gggaatggag ttacaattat caggcaaggg
      781 agtaattaaa atgaaggcaa tacaatgttg ttatttcctt gctttagtct ccagtcattt
      841 aatggctgga gttatgggtg atgataatat atttttaagt aattcggatt cagctgcttt
      901 atattgtgtg gctaaaatat ttccagatgg agaaaatatt ttaatatata aagtaaatat
      961 aattaatgct agttcaaaaa aagatgtttt ttttatgaaa gcccaacatc aacccacaat
     1021 gacataatgg ggggcggggg aatgtcatat gcctatgaat atgatgtaaa atataatata
     1081 tgtcatttga gaaaaaagag cagaatgagg taaaaatctt tggctgtgta ttcagtctgg
     1141 taatcatcca aggaaagctt atcagaaggg tgatgactcc aattagtaga acatatattt
     1201 tcgataaaag caccgatgac tttttcatga agttctacag aacgcgagaa acagatggtt
     1261 ctgcgggcca gacgtttgat tcgggttctc agggtcagat tgttacgctc aatgtgctga
     1321 gtaaaaatct taccagtcag atgcttctcc ttcggtaact ctctagcgta actaccccat
     1381 tcgtcagtgg tcaacatgcc gatggtaaaa gggtaagcag cgcccgcaat tcacgacagg
     1441 tttcgtcagt ccgggggcca aaagtgtagg ccagaacacc accagttttc gtgttgtaca
     1501 cgtaccagag ccagtgctgt cgcgctttac tgccgacaaa gctccattgc tgactaagtt
     1561 tgcagatgag tgccacatca gcatgagcga ccggggatga cgttattctt cgtggcgaga
     1621 gttttttaaa gttcggatga ctgtattaat gccgattttc agcgtacggg cagtatcgcg
     1681 aaccccggca ccgttgaaag tcatttcagc gatttggtct ttaactcccg gtttacgggg
     1741 ctcgtaacag taactgagct gaaatacccg gtgacagtca cggccgcgga atcgctcatg
     1801 tctttttggg ttctgcccgt gacggtaaac ctgaacggac tggcaacggg ggcaatgaag
     1861 gttaactgtg accatgaaac aaccttaaaa gcgagtatta tatccttaat tcaactaatt
     1921 ggaggcatca ccgtttaccg gagcgacagc ttgcggaaag gtcgtgcaac tacaaagcaa
     1981 caaacagact acggcgggaa ttattttttt catcatccat taccacgaaa acagcggcca
     2041 ctctttggcg actgtggtga gttgtggcgc gtcgaaaacg gcttgtggtg aaactaccgt
     2101 ttcctcaagc ggaatatttt tgccgtagcg ttctttgagc ttagcctgga ccagcgggtt
     2161 gctgagatcg aactctttca acttctgctg ctcgcccacg cgcattccca gcgcgttctg
     2221 gtaaaccttc cacaccactt ccgaacagta ctggcgatcg tcgctccatg agaagctgaa
     2281 atcatacggt ttaccgagat aacgttttgc cgtttgcgcc agtttttgct gttgttcaac
     2341 actcagtccg ccttcaacgc ggcgaacaac gtatttgccc ttttcaccat gggcgatcca
     2401 ctgcttgagc ggggggtatt tcaccgggcc gaccgcttca aaaacgtagg gctttttgtt
     2461 gcgtatcacc agcataccgg tgtggctata atcggaatgg gtcgccagtt ggatcgcttt
     2521 actttgcgat gagcgagaga tctgaaagat gatgtcgccg gtttgtggct gccaggcaaa
     2581 ggcaggtaca gaaaccagca ggctgaggat cagcagcctg tttttcatag ttaaacgtcc
     2641 atgtataaaa agcggtgggt cgcagacaac gtgctcgttg tttatgccgg atgcggcgtg
     2701 aacgccttat ccggcctaca agttcgtgca aattcaataa attgcaatat gacgtaggcc
     2761 tgataagcgt agcgcatcag gcgattccac tccgcgccgc tcttttttgc tttagtattc
     2821 ccacgtctcc gggttaatcc ccatctcacg catgatctct tttgccgctt ccgggatttc
     2881 gtcgctgcgc tctttacgca gatcggcatc atccggcaaa ggttgcccgg taaaggcatg
     2941 cagaaacgct tcgcacagca gctcgctgtt ggtagcgtga cgcaggttgt tcacctgacg
     3001 acgcgtgcgt tcatcggtga ggatttttaa caccttaaga ggaatggaaa ccgtaatctt
     3061 tttgacttgt tcactcttct tgccgtgctc agcgtatggg ctgatatatt cgccgctcca
     3121 ttcagccatg agatacttaa tcctcttcgt caataaattg agaccagacc acacaattta
     3181 tgtgggtact gaccgtaaac ccgcatagtt taccgtacag gcgttaccgt gacatcgtgt
     3241 aatgcacctg tcggcgtgat aatgcatata attttaacgg ctatttggga tttgctcaat
     3301 ctatacgcaa agaagtttag atgtccagat gtattgacgt ccattaacac aatgtttact
     3361 ctggtgcctg acatttcacc gacaaagccc aggaaacttc atcacatgac gcgtaaacag
     3421 gccaccatcg cagtgcgtag cgggttaaat gacgacgaac agtatggttg cgttgtccct
     3481 cccatccatc tttccagcac ctataacttt accggattta atgaaccgcg cgcgcatgat
     3541 tactcgcgtc gcggcaaccc aacgcgcgat gtggttcagc gtgcgctggc agaactggaa
     3601 ggcggtgctg gcgcagtact tactaatacc ggcatgtccg cgattcacct ggtaacgacc
     3661 gtctttttga aacctggcga tctgctggtt gcgccgcacg actgctacgg cggtagctat
     3721 cgcctgttcg acagtctggc gaaacgcggt tgctatcgcg tgttgtttgt tgatcaaggc
     3781 gatgaacagg cattacgggc agcgctggca gaaaaaccca aactggtact ggtagaaagc
     3841 ccaagtaatc cattgttacg cgtcgtggat attgcgaaaa tctgccatct ggcaagggaa
     3901 gtcggggcgg tgagcgtggt ggataacacc ttcttaagcc cggcattaca aaatccgctg
     3961 gcattaggtg ccgatctggt gttgcattca tgcacgaaat atctgaacgg tcactctgac
     4021 gtggtggctg gcgtggtgat tgctaaagac ccggacgttg tcactgaact tgcctggtgg
     4081 gcaaacaata ttggcgtgac gggcggcgcg tttgacagct atctgctgct acgcgggttg
     4141 cgaacgctgg tgccgcgtat ggagctggcg cagcgcaacg cgcaggcgat tgtgaaatat
     4201 ctgcaaaccc agccgttggt gaaaaaactg tatcacccgt cgctgccgga aaatcagggg
     4261 catgaaattg ccgcgcgtca gcaaaaaggc tttggcgcaa tgttgagttt tgaactggat
     4321 ggcgatgagc agacgctgcg tcgtttcctg agcgggctgt cgttgtttac gctggcggaa
     4381 tcattagggg gagtggaaag tttaatctct cacgccgcaa ccatgacaca tgcaggcatg
     4441 gcaccagaag cgcgtgctgc cgccgggatc tccgagacgc tgctgcgtat ctccaccggt
     4501 attgaagatg gcgaagattt aattgccgac ctggaaaatg gcttccgggc tgcaaacaag
     4561 gggtaaaaat gagtgtgatt gcgcaggcag gggcgaaagg tcgtcagctg cataaatttg
     4621 gtggcagtag tctggctgat gtgaagtgtt atttgcgtgt cgcgggcatt atggcggagt
     4681 actctcagcc tgacgatatg atggtggttt ccgccgccgg tagcaccact aaccagttga
     4741 ttaactggtt gaaactaagc cagaccgatc gtctctctgc gcatcaggtt caacaaacgc
     4801 tgcgtcgcta tcagtgcgat ctgattagcg gtctgctacc cgctgaagaa gccgatagcc
     4861 tcattagcgc ttttgtcagc gatctggaac gcctggcggc gctgctcgac agcggtatta
     4921 acgacgcagt gtatgcggaa gtggtgggcc acggggaagt atggtcggca cgcctgatgt
     4981 ctgcggtact taatcaacaa gggctgccag cggcctggct tgatgcccgc gagtttttac
     5041 gcgctgaacg cgccgcacaa ccgcaggttg atgaagggct ttcttacccg ttgctgcaac
     5101 agctactggt gcaacatccg ggcaaacgtc tggtggtgac cgggtttatc agccgtaaca
     5161 acgccggtga aacggtgctg ctggggcgta acggttccga ctattccgcg acacaaatcg
     5221 gtgcgctggc gggtgtttct cgtgtaacca tctggagcga cgtcgccggg gtatacagtg
     5281 ccgacccgcg taaagtgaaa gatgcctgtc tgctgccgtt gctgcgtctg gatgaagcca
     5341 gcgaactggc tcgtctggcg gctcccgttc ttcacgcccg tactttacag ccggtttctg
     5401 gcagcgaaat cgacctgcaa ctgcgctgta gctacacgcc ggatcaaggt tccacgcgca
     5461 ttgaacgcgt gctggcctcc ggtactggtg cgcgtattgt caccagccac gatgatgtct
     5521 gtttgattga gtttcaggtg cccgccagtc aggatttcaa actggcgcat aaagagatcg
     5581 accaaatcct gaaacgcgcg caggtacgcc cgctggcggt tggcgtacat aacgatcgcc
     5641 agttgctgca attttgctac acctcagaag tggccgacag tgcgctgaaa atcctcgacg
     5701 aagcggggtt acctggcgaa ctgcgcctgc gtcaggggct ggcgctggtg gcgatggtcg
     5761 gtgcaggcgt cacccgtaac ccgctgcatt gccaccgctt ctggcagcaa ctgaaaggcc
     5821 agccggtcga atttacctgg cagtccgatg acggcatcag cctggtggca gtactgcgca
     5881 ccggcccgac cgaaagcctg attcaggggc tgcatcagtc cgtcttccgc gcagaaaaac
     5941 gcatcggcct ggtattgttc ggtaagggca atatcggttc ccgttggctg gaactgttcg
     6001 cccgtgagca gagcacgctt tcggcacgta ccgactttga atttgtgctg gcaggcgtgg
     6061 tggacagccg ccgcagtctg ttgagctatg acgggctgga cgccagccgc gcgttagcct
     6121 tcttcaacga tgaagcggtt gagcaggatg aagagtcgtt gttcctgtgg atgcgcgccc
     6181 atccgtatga tgatttagtg gtgctggacg ttaccgccag ccagcagctt gctgatcagt
     6241 atcttgattt cgccagccac ggtttccacg tcatcagcgc caacaaactg gcgggagcca
     6301 gcgacagcaa taaatatcgc cagatccacg acgccttcga aaaaaccggg cgtcactggc
     6361 tgtacaatgc caccgtcggt gcgggcttac cgatcaacca caccgtgcgc gatctgatcg
     6421 acagcggcga tactattttg tcgatcagcg ggatcttctc cggcacgctc tcctggttgt
     6481 tcctgcaatt cgacggtagc gtgccgttta ccgagctggt ggatcaggcg tggcagcagg
     6541 gcttaaccga acctgacccg cgtgacgatc tctctggcaa agacgtgatg cgcaagctgg
     6601 tgattctggc gcgtgaagca ggttacaaca tcgaaccgga tcaggtacgc gtggagtcgc
     6661 tggtgcctgc ccattgcgaa ggcggcagca tcgaccattt ctttgaaaat ggcgatgaac
     6721 ttaacgagca gatggtgcaa cggctggaag cggcccgcga aatggggctg gtgctgcgct
     6781 acgtggcgcg tttcgatgcc aatggcaaag cgcgtgtggg cgtggaagcg gtgcgtgaag
     6841 accatccgct ggcatcactg ctgccgtgcg ataacgtctt tgccatcgaa agccgctggt
     6901 atcgcgataa tccgctggtg atccgtgggc ctggcgctgg gcgcgacgtc accgccgggg
     6961 cgattcagtc ggatatcaac cggctggcac agttgttgta atttcagaaa tttaataatg
     7021 cccggtactc atgttttcgg gtttatggtt tctaatgaaa tatattgaat tatcatagga
     7081 ttaggccgga ttaagcgttt acgacgaatc cggcaagaag caataagtac atggttagtt
     7141 tatatttgca gtccggtttg ctttgcatac cggattttct ttttcttacc atcctgaagt
     7201 tttttcatct tccctgattt ttcctcacca tcattggtca tttttcggtt gacgcccttc
     7261 ggcttttcct tcatctttac atctggacgt ctaaacggat agatgtgcac aacacaacat
     7321 ataactacaa gcgattgatg aggtaaggta tgagcttttt tcacgccagc cagcgggatg
     7381 ccctgaatca gagcctggca gaagtccagg ggcagattaa cgtttcgttc gagtttttcc
     7441 cgccgcgtac cagtgaaatg gagcagaccc tgtggaactc catcgatcgc cttagcagcc
     7501 tgaaaccgaa gtttgtatcg gtgacctatg gcgcgaactc cggcgagcgc gaccgtacgc
     7561 acagcattat taaaggcatt aaagatcgca ctggtctgga agcggcaccg catcttactt
     7621 gcattgatgc gacgcccgac gagttgcgca ccattgcacg cgactactgg aataacggta
     7681 ttcgtcatat cgtggcgctg cgtggcgatc tgccgccggg aagtggtaag ccagaaatgt
     7741 atgcttctga cctggtgacg ctattaaaag aagtggcaga tttcgatatc tccgtggcgg
     7801 cgtatccgga agttcacccg gaagcaaaaa gcgctcaggc ggatttgctt aatctgaaac
     7861 gcaaagtcga tgccggagcc aaccgcgcga ttactcagtt cttcttcgat gtcgaaagct
     7921 acctgcgttt tcgtgaccgc tgtgtatcgg cgggcattga tgtggaaatt attccgggaa
     7981 ttttgccggt atctaacttt aaacaggcga agaaatttgc cgatatgacc aacgtgcgta
     8041 ttccggcgtg gatggcgcaa atgttcgacg gcctggatga tgatgccgaa acccgcaaac
     8101 tggtgggcgc gaatattgcc atggatatgg tgaagatttt aagccgtgaa ggagtgaaag
     8161 atttccactt ctatacgctt aaccgcgctg aaatgagtta cgcgatttgc catacgctgg
     8221 gtgttcgacc tggtttataa atagtgtggc ttttgtgaaa atcacacagt gatcacaaat
     8281 tttaaacaga gcacaaaatg ctgcctcgaa atgagggcgg gaaaataagg ttgtcagcct
     8341 tgttttctcc ctcattactt gaaggatatg aagctaaaac ccttttttat aaagcatttg
     8401 tccgaattcg gacataatca aaaaagctta attacgatca atttgaccta catctcttta
     8461 accaacaata tgtaagatct caactatcgc atccgtggat taattcaatt ataacttctc
     8521 tctaacgctg tgtatcgtaa cggtaacact gtagagggga gcacattgat gagcacgtca
     8581 gacgatatcc ataacacgac agccactggc aaatgcccgt tccatcaggg cggtcacgac
     8641 cagagcgcgg gggcgggcac aaccactcgc gactggtggc caaatcaact ccgtgttgac
     8701 ctgttaaacc aacattctaa tcgttctaac ccgctgggtg aggactttga ctaccgcaaa
     8761 gaattcagca aattagatta ctacggcctg aaaaaagatc tgaaagccct gctgacagaa
     8821 tctcagccgt ggtggccagc cgactggggc agctacgccg gtctgtttat tcgtatggcc
     8881 tggcacggcg caggtactta ccgttcaatc gatggacgcg gcggcgcggg tcgtggtcag
     8941 caacgttttg caccgctgaa ctcctggccg gataacgtaa gcctcgataa agcgcgtcgc
     9001 ctgttgtggc caatcaaaca gaaatatggt cagaaaatct cctgggccga cctgtttatc
     9061 ctcgcaggta acgtggcgct ggaaaactcc ggcttccgta ccttcggttt tggtgccggt
     9121 cgtgaagacg tctgggaacc ggatctggat gttaactggg gtgatgaaaa agcctggctg
     9181 actcaccgtc atccggaagc gctggcgaaa gcaccgctgg gtgcaaccga gatgggcctg
     9241 atttacgtta acccggaagg cccggatcac agcggcgaac cgctttctgc ggcagcagct
     9301 atccgcgcga ccttcggcaa catgggcatg aacgacgaag aaaccgtggc gctgattgcg
     9361 ggtggtcata cgctgggtaa aacccacggt gccggtccga catcaaacgt aggtcctgat
     9421 ccagaagctg caccgattga agaacaaggt ttaggctggg cgagcactta cggcagcggc
     9481 gttggcgcag atgccattac ctctggtctg gaagttgtct ggacccagac gccgacccag
     9541 tggagcaact atttcttcga aaacctgttc aaatatgagt gggtacagac ccgcagtccg
     9601 gctggcgcaa tccagttcga agcggtagac gcaccggaaa ttatcccgga tccattcgat
     9661 ccgtcgaaga aacgtaaacc gacgatgctg gtgaccgacc tgacgctgcg ttttgatcct
     9721 gagttcgaga agatctctcg tcgtttcctc aacgatccgc aggcgttcaa cgaagccttt
     9781 gcccgtgcct ggttcaaact gacgcacagg gatatggggc cgaaatctcg ctacatcggg
     9841 ccggaagtgc cgaaagaaga tctgatctgg caagatccgc tgccgcagcc gatctacaac
     9901 ccgaccgagc aggacattat cgatctgaaa ttcgcgattg cggattctgg tctgtctgtt
     9961 agtgagcggg tatcggtggc ctgggcatct gcttctacct tccgtggtgg cgacaaacgc
    10021 ggtggtgcca acggtgcgcg tctggcatta atgccgcagc gcgactggga tgtgaacgcc
    10081 gcagccgttc gtgctctgcc tgttctggag aaaatccaga aagagtctgg taaagcctcg
    10141 ctggcggata tcatcgtgct ggctggtgtg gttggtgttg agaaagccgc aagcgccgca
    10201 ggtttgagca ttcatgtacc gtttgcgccg ggccgcgttg atgcgcgtca ggatcagact
    10261 gacattgaga tgttcgagct gctggagcca attgctgacg gtttccgtaa ctatcgcgct
    10321 cgtctggatg tttccaccac cgagtcactg ctgatcgaca aagcccagca actgaccctg
    10381 accgcgccgg aaatgaccgc gctggtgggc gggatgcgcg tactgggtgc caacttcgat
    10441 ggcagcaaaa acggcgtctt cactgaccgc gttggcgtat tgagcaatga cttcttcgtg
    10501 aacttgctgg atatgcgtta cgagtggaaa gcgaccgacg aatcgaaaga gctgtttgaa
    10561 ggccgtgacc gcgaaaccgg cgaagtgaaa tacactgcca gccgtgcgga tctggtattt
    10621 ggttctaact ctgtcctgcg tgcggtggct gaagtttacg ccagcagcga tgctcacgag
    10681 aagtttgtta aagacttcgt ggcggcatgg gtgaaagtga tgaacctcga ccgtttcgac
    10741 ctgctgtaat ctgaccttct tcagcgactg cctttcaggc agtcgctgaa gtttctttac
    10801 cggcgtatag tgtccacagg aaaactacac actggatctc tcatgtctgc cgcaggaaag
    10861 agcaacccac tggcaatcag tggcctggtt gtgctcacac ttatctggag ttatagctgg
    10921 attttcatga agcaagtcac cagctacatc ggtgccttcg actttaccgc cttacgctgc
    10981 attttcggcg ctctcgtttt attcatcgtc cttttattac gtggtcgcgg aatgcgcccg
    11041 acaccgttta aatacacctt agccattgcc ctgttacaaa cctgcgggat ggttggcctg
    11101 gcgcagtggg cgctggtcag cggcggagcg gggaaggtgg cgatcctaag ctataccatg
    11161 ccgttctggg tggtgatttt cgccgcgttg tttctcggtg aacgcctgcg acgtgggcaa
    11221 tatttcgcga tactgattgc cgctttcggc ttatttttgg tgttgcagcc gtggcaactc
    11281 gatttctctt cgatgaaaag tgccatgctg gcaatcctct ccggcgtcag ttggggggcg
    11341 agcgcgattg ttgctaaacg tctgtatgcc cgtcatccgc gcgtggattt attgtcgtta
    11401 acatcctggc agatgctcta tgcggcgctg gtgatgagtg tggtcgcttt actggtgccg
    11461 caacgtgaaa ttgactggca gcccaccgtg ttctgggcgc tggcctacag tgcgattctg
    11521 gcgacggcac tggcgtggag cttatggttg tttgtattga aaaacttgcc tgccagtatt
    11581 gccagcttaa gcacactggc cgttcccgtt tgcggcgtac tcttttcctg gtggctgctc
    11641 ggcgagaatc cgggggccgt tgaaggtagc ggtattgtgc tgattgtgat ggcactggcg
    11701 ctggtgagcc gtaagaaaaa agaagccgtt agtgtaaaaa ggatctgaat tttttcttca
    11761 tgtggggcga tcgcttattt aacaaaataa cgataatgcc ccaccatgcg ccagctaaac
    11821 agcacatctt cttcctgcgc acctgcgcca atgttatgta tcaccagcgg cgtaccgtcg
    11881 cgggcgaagc catctgaaac caccccaata tgtgccagcc cgttatctaa tcgccaggag
    11941 acaatatcgc ccgcttgata gtcactgggg ttcttgctga tggggcgtgt tttatcgtgg
    12001 tgggtaaacc aggtttccag attaggcacc cgacggtgat cgatgttgct gtccgggcgc
    12061 tttaactgcc atttttgcgg gtactcggca aaattcttcg ccatatcttc gtgcaccagt
    12121 ttctgcaaat cgaccttctg gctgcgcaat gcacggatca ccacatcgga acataccccg
    12181 cgtttttgcg ggacatcacc gccaggatag gtaagctgca tatacgccgg atcgtaaaat
    12241 agcgtgctgc cgatttgctg tctggcaccg tctgcgatgg caaggttggt attggcctgg
    12301 atttgtacca cggttggtgg aacggcggga gattttaagg agtggctggt aaatgccgtt
    12361 agcaggctga gcagcgccag tgaagctttc atctggttcg tccgtgaaaa taagagtcac
    12421 agattcgacc ttccgggata acgattgttc tgtagagaaa atgtccagaa gcggataaaa
    12481 aactcctgaa caacgtggga gtttcagatt actggcacag agtgccggat gcggcgtgaa
    12541 cgccttatct gtcctaaaaa aaaagaaaat tcaatatatt gcagagattg cgtaggcctg
    12601 ataagcgtag cgcatcaggc aattttgcgt tcaaactccc ggacaagccg ggagtttgga
    12661 gtaggttatt cccactcttg caggaaacgc tgaccgtact ggtcggctac cagcagagcg
    12721 gcgtaaacct gatctggcgt cgcgccgcca ggcatgttgt ggatggtttc acattctgca
    12781 catgccgctt cagccacaat tcgcattttc gccgggacat cttctttaat atccagttga
    12841 gcgagagtta ttggcaaacc taccgcatgg ctaagtgcag ctacggtttc gatttcctca
    12901 accggtgcgt tttccagaac cagctgcgtc agcgtaccaa atgccacttt ttcaccgtga
    12961 taatagtgat gcgcgtccgg gatagcggtc aggccgttat gcactgcgtg cgccgcagcc
    13021 agaccaccac tttcaaaacc aacaccgctc aaataggtgt tcgcttcaat cacgcgctcc
    13081 agcgccggag tcactacatg ctgttcggca gcaagcatcg ctttttcgcc ttcttccagc
    13141 agggtgttgt agcacagttc agccagtgcc agcgcagcct gggtgcactt gccgcccgcc
    13201 atggtggtcg cgccgctacg agagcaggca cgcgcttcaa accaggttgc cagcgcatcg
    13261 ccgatacccg ccgctaacag acgtgcaggt gcgccagcga cgattttggt gtcgacaatg
    13321 accatattcg ggttatttgg caacagcaga tagcggtcaa actcaccctc atcggtgtag
    13381 ataacagaca atgcgctgca cggtgcatcg gtagaggcga tagtcggtgc gatcgctacc
    13441 ggaacaccca tgaaatgtgc cagtgctttg gctgtatcga gggtttttcc gccaccgata
    13501 ccgagaattg cgccacactg cgcagtctcc gcgatgccac gcagacggtc gatctcattt
    13561 tgcgaacatc caccgccaaa cggcgcaatt tctactacca gtccagcatc tttaaagctt
    13621 ttctcgacag tggattgagc aaaacctaaa acaaatttgt cacccaccac taaccagcgt
    13681 tctgccagcg gcttcaggta ttcgcccaga cgattaatca catcagcgcc ctggatgtat
    13741 ttacccggtg attgaataat gcggtccata attgctcctt tagagatgag tagtgccaaa
    13801 tgcggcattc cagtcgtgtt cgaacttctc tatagctgac tctaccgcag gggtgttgag
    13861 catttgttgc gctacatcta agggcagggt gatggattca catcctgcca gtaaacagtc
    13921 cagcgcctga cgcggcgttt taaagctggc tgccagcacc atgctttctg gcgcgtgcat
    13981 ttctaacagc gcttgcagct cctgaaccgt acgaatgccg tctccgccct gggcatctac
    14041 gcggttaaca tacggagcaa cgtattttgc ccctgccagc gcggccagta acccttgtgc
    14101 agcactgtac actgcggtgc caagcgtagt aataccctct tttttcagta ttttaattgc
    14161 tgccagacct tcggaagtca ccgggatttt caccacaata cccggaatag cgtcgcgcag
    14221 gcgcttcgct tcttccacca tcccctgcgc gtcgcggctc atggtctgag caaacagaat
    14281 gccctcatca ccaatcgctt tttgcagacg cggcagcact tcccatatgg actccttgct
    14341 ggcagcgata atgctcgggt tagttgtcac cccggcaatg gggaatatgc gtgccagacg
    14401 ttcgacttct gcgacgttag cggtgtccag atacagttcc atgatgtttc cttttacagt
    14461 tccagttcat gttgcagcag gctggcgata gcgtcggcag aggcggcgtt aaccagcgcg
    14521 ttacggaatt cttcgtgcat gatgcggcga gcgaggcgcg agaaaatgcg catgtgctga
    14581 tcgcccgcag cgtgtttgtt cagggttaac atgatgatga attgcgcttc atcatcaccc
    14641 cagcgcaccg gcgcttgcag acgcgcaacg ctgatggtgg attgctcaat gtgttctgat
    14701 ttgctgtgtg gaatggcaaa actaaagccc agaccggtag agaaaacggc ctcgcgcgcc
    14761 cacaagtcgg cttccagttt acgcggatag cggcagcgcc ctgccagcag caggttatcg
    14821 gtcatgcctt tgagcacttc ttctttgctg cgccagtcgc tttccagtgt gatgcactcg
    14881 gcggtgacca gcggtgcgtc ttgttgggtc atgcggaatt gcgccagcag gtgctccact
    14941 tccagcgaag tacggcaggc cattgcctgg ttgagcaact tgcggcactc acggctatca
    15001 agttgcgcca tccgcgcttt cgccgccgga attgatggtg cgctcatgct gagttcatcc
    15061 agccctaagc cgaccagcaa cggcagcacg gaacctttcg ctcccagctc accgcacaga
    15121 ccaatccatt taccctggcg atgcaccgcc tgcacggcgt aatcgagcgc ccacaagaat
    15181 gccggattca ggctgttgta gtgacgagta accttagcgt tatcgcgatc cactgccagc
    15241 agatattgcg tcaggtcatt actaccaata ctaaagaaat caatctcttc gcagcattga
    15301 tcgatgatga acatcaccga cggcacttcc agcataatgc cgagctggat cttctcatca
    15361 aacggaatgt gttcgttacg tagttgctgt ttggcttctg ccagtttttc tttcacccat
    15421 aagatctctt ccattgagga gatcatcggg atcatgattt tcaggctgcc gtgagcggag
    15481 gcgcgcagga tcgagcgtag ttgtgtggta aataacgacg cgtattcttc ataaatacgc
    15541 acggcgcgat aaccgaggaa cgggtttgcc tctgcgggaa tgtttagata atcaacgggt
    15601 ttgtcgccgc caatgtccat agtgcgcaca ataatgctgc gtccgttggc agactccagc
    15661 gcctgacaaa aaatgttgta cagctcgctt tcgcccggtg cactggtgcg atccatatag
    15721 agcatttcag tgcggaacaa accaacgcct tccgcaccat tgccgaatgc ggcctgcgct
    15781 tccacggagt gagcgatgtt agcggcaatt tcaatgcgga taccgtcagc ggtacgggct
    15841 tgttgggtca gccagacacg ctgttgctca cgcagggcgt cctgtacgcg ggcttcttgc
    15901 tgataataac gagctacggc ttctcctggc tcaaccacaa tcgccccggc gttgccgtcg
    15961 atataaatcg tttgatgctg ccacggagtg agggcatcaa tatccacacc aaccagcgtc
    16021 ggaatgttga acgaacgggc aaggatcacc gtatgtgagg tggtgccgcc acttttcagc
    16081 aacagacctt tgaggtgatt tttatccagt tcgaggaact ggctgggggt cagttcatca
    16141 gccatacaaa tggcggactg cgtcagtttg cccggtgccg ggaagcgttg ctcaccgtag
    16201 atttgctgga gtaactggaa gcagacgtcg cgtacgtcca gggcacgttc ttgcaggtag
    16261 ctgctgctgg aacgggcgaa ctcttcgcaa aagtgattcg cactggcgac aatcgcttcg
    16321 gcgcagctta atccctcgct gacgcctgcc agtaagtgct gacgtaaaga tgtatcgcca
    16381 gccagggatc ggtgagcttc cagtatcgcg ctggtagcac cgtcgctatc cagcagacga
    16441 aactcaatgt ttttcagtac cagcgtcagg ccgttttcca gtgtggattg ctcggcgtca
    16501 acgtctttgg ctgcgggaag attactcagc gcattgagat ctaaagaaga gatcggcgtc
    16561 agaatgccgc ctgcactacc gctgcacacg gtgcgggcgc ggataatttg cggattcaga
    16621 ttggtcagtg aaaccggcag tggttccagt tcgtcagatt taacttccgc cagcggcgca
    16681 tcgcagtggg ggaattcatc gcgcagccat tggcttaaac gctggtgcgc ttcctgttcg
    16741 tcggccccgg aaatcagtaa ctggcagtta tcgcccgcca gcgtatcggt gccaatcagc
    16801 gcaagggcgc ttttggcgtt gcccttgcgg tcagtgtgca ggttatgcca ctcaatttgt
    16861 gatgaaaaag tattacacag cgtttcaacg tggcttgccg gacgcgcatg tacgccgtta
    16921 ggtagctcac aaataaattc cacaatcagg gccattgcct ctctcccggt acggttttct
    16981 gtttacagga taagagcgca cggcaacggc ctgccatgtg acaaatctgc caaaagctgg
    17041 acaaatgtaa tgtaaccgtc aatttgcgac gcgtctcaca agacgccgtt ttgcggcatg
    17101 cttccggttt atcgcaagtt atgaggcgga tcgcattttt gtactgatat tacaaaagtc
    17161 cagtaaatgg cctttttatc cactgtttgc tccgcctgcg attgcctatt gtttcctcca
    17221 tcaaaaatat ggacatgggc tttcgcaagc ccgggagcag cttatgaatg agttggtgca
    17281 gatcctgaaa aatacccgtc agcatttaat gacgggcgtt tcacacatga ttcccttcgt
    17341 ggtatcgggc ggtattttgc tggcggtttc cgtcatgttg tatggcaaag gcgcagtgcc
    17401 ggatgccgta gccgatccaa atctgaaaaa actgtttgat atcggcgttg cgggcttgac
    17461 gctgatggtg cctttcctcg ccgcttacat tggttattcc attgcagaac gttctgcgct
    17521 ggctccatgc gctatcggta cctgggttgg taacagcttt ggtgcgggct tctttggtgc
    17581 actgatcgcc gggattatcg gcggcatcgt ggtgcattac ctgaagaaaa ttccggtgca
    17641 taaagttctg cgctcggtga tgcctatctt cattattcct atcgtcggca cactgattac
    17701 cgcaggcatc atgatgtggg gcttgggcga gcctgtaggg gcgttgacca acagcctgac
    17761 tcagtggctt caggggatgc agcagggcag cattgttatg ctggcggtga tcatgggtct
    17821 gatgctggcg ttcgatatgg gcggtccggt taacaaagtg gcctatgcct tcatgctgat
    17881 ttgcgttgct cagggtgttt ataccgtggt ggccatcgct gccgtgggta tttgtatccc
    17941 gccgctgggg atggggctgg cgacgctgat tggtcgtaaa aatttctccg cagaagagcg
    18001 cgaaaccggc aaagcggcac tggtgatggg ctgtgttggc gttactgaag gggcgattcc
    18061 tttcgccgct gccgatccgc tgcgtgtcat tccttccatc atggtcggtt cagtttgtgg
    18121 tgcagtaact gcggcgctgg tcggtgcgca gtgctatgca ggctggggtg gtctgattgt
    18181 gctgccggtg gttgaaggca agctgggtta tatcgcagca gtggctgtcg gagcagtggt
    18241 gacggctgtt tgtgtgaacg tgctgaaaag tctggcgcgt aaaaatgggt cttcgactga
    18301 tgaaaaagaa gacgacctgg atttggattt tgaaattaat taattgagga acgagccatg
    18361 acgaaaatta ttgcagtaac cgcatgtcct tcaggtgttg cccataccta tatggcagca
    18421 gaggcgctgg aaagtgccgc gaaagcgaaa ggctgggaag tgaaagtaga aacctagggg
    18481 tcaattggtc tggaaaatga actgactgcg gaagatgtgg cgagcgctga tatggtgatt
    18541 ctgaccaaag atatcggcat caagtttgaa gagcgttttg ctggcaagac catcgtgcgc
    18601 gtcaacatca gcgatgcagt aaaacgtgct gacgccatta tgagcaaaat tgaagctcac
    18661 ctggcgcaaa ccgcttaagt tcttctcccg ctcgcaaggg cgggttcgct ttcccacagg
    18721 agttcctcat gacgaatcgt atctctcgcc tcaaaactgc actgtttgcc aatacccgtg
    18781 aaatctcgct ggagcgggcg ctgctttata ccgccagcca tcggcaaacc gaaggcgaac
    18841 cggtgatatt gcgccgggcg aaagcaacag cgtatatcct tgaacatgtt gaaatttcga
    18901 ttcgtgatga agaactgatt gccggtaacc gcaccgtaaa accgcgcgcc gggattatgt
    18961 cgccggaaat ggacccttac tggctgctga aagagctgga tcaattcccg acgcgtccgc
    19021 aggaccgctt tgctatcagc gaagaagata aacgtatcta ccgtgaagag ttgttcccgt
    19081 actgggaaaa acgttcgatg aaagatttca tcaacgggca gatgacagat gaagtaaaag
    19141 ccgcgaccaa cacgcagatt ttcagcatca accagacgga taaaggccag gggcacatta
    19201 ttattgatta cccacgcctg ttgaatcacg gtctgggtga gctggtggca cagatgcagc
    19261 aacattgtca gcaacagccg gagaatcact tttatcaggc cgcgttgtta ctgctggaag
    19321 cctcgcagaa acacattttg cgttacgccg aactggcgga aacgatggcg gcaaactgca
    19381 cagatgcaca gcgtcgcgaa gaactgctga ctattgcgga gatctctcgc cataacgccg
    19441 aacataagcc gcagacgttc tggcaggcgt gccagttatt ctggtacatg aacatcattc
    19501 tgcaatacga atccaacgcc agttcgctgt ctttagggcg cttcgaccag tatatgttgc
    19561 cgttctatca ggcatcttta acccagggcg aagatccggc gttcctgaaa gaactgctcg
    19621 aatctttatg ggtgaaatgc aacgacatcg tgctgttgcg ctccaccagc agcgcgcgtt
    19681 atttcgcagg tttcccgacc ggctataccg cactgctcgg cgggttaacc gagaacggac
    19741 gtagcgcggt gaacgtgctt tcgttccttt gccttgacgc ctatcaaagc gtgcaattac
    19801 cgcaaccgaa cctcggcgtg cgcactaacg ccttgatcga cacgccgttc ctgatgaaaa
    19861 ccgccgaaac cattcgcctc ggcaccggta ttccgcaaat ctttaacgat gaagtggtgg
    19921 taccagcgtt cctcaaccgt ggcgtttcgc tggaagatgc gcgcgactat tccgtagtgg
    19981 gctgtgtgga attatctatt cccggcagaa cctacggctt gcatgacatc gcgatgttta
    20041 atctgctgaa agtgatggaa atctgcctgc atgaaaatga aggcaatgct gcgctgactt
    20101 atgaaggttt actggagcag atccgcgcca agatcagcca ctacatcacc ctgatggttg
    20161 agggcagcaa tatttgtgat atcggccatc gcgactgggc acctgtaccg ctgctctcgt
    20221 cttttatcag cgattgtctg gaaaaaggtc gcgatattac cgatggcggc gcgcgttata
    20281 acttctccgg cgtacagggg atcggtatcg ccaacctgag cgattctctc catgcgttga
    20341 aagggatggt ttttgagcaa cagcgtttaa gttttgacga attgctgtcg gtattaaaag
    20401 ccaactttgc aacgccagaa ggcgaaaaag tccgtgctcg cttaattaac cgcttcgaga
    20461 aatacggtaa cgatatcgac gaggtggata acatcagcgc cgaactgtta cgccactact
    20521 gcaaagaagt ggaaaaatac cagaacccgc gcgcggttac ttcacgccgg gatcgtatac
    20581 cgtttctgct cacgtcccgt tgggatcggt ggttggcgcg acgccagacg gtcgttttgc
    20641 cggggaacaa ctggcagacg gcggcttgtc acctatgctg ggccaggacg cacaagggcc
    20701 aacagcggta ctgaagtcag tcagtaagct cgataatacg ctgctgtcta acggtacgtt
    20761 gctgaacgtg aaattcactc cggcgaccct ggaaggtgaa gcgggattac gcaaactggc
    20821 cgacttctta cgggcgttta cccagcttaa gttgcagcat attcagttta acgtggtgaa
    20881 cgccgacacg ttgcgggaag cgcaacagcg cccacaagat tatgccgggc tggtggtgcg
    20941 cgttgccgga tacagcgcct tctttgtcga actgtcgaag gagatccagg atgacatcat
    21001 ccgccggaca gcgcatcagc tgtaacgttg tggaaacgcg ccgcgatgat gtggcgcgca
    21061 ttttcaacat tcagcgttat tcactgaatg acggcgaggg cattcgtacg gtggtctttt
    21121 ttaaaggctg tccgcatctt tgcccgtggt gtgctaatcc ggagtcgatc tccggcaaaa
    21181 tccagacggt acgcagagag gcgaaatgtc tgcactgtgc gaaatgtttg cgtgatgcgg
    21241 atgaatgccc ctccggggcg tttgaacgga ttggtcgcga tatcagcctt gacgctctgg
    21301 aacgggaagt gatgaaagat gacattttct ttcgcacgtc cggcggcggc gtcacgcttt
    21361 ctggcggcga agtgttaatg caggcggagt ttgctacccg ttttttacag cgactgcggc
    21421 tgtggggtgt gtcatgcgcc attgaaactg ccggagacgc gccagcaagc aagctgttac
    21481 cgctggcgaa attgtgcgat gaagtgctgt tcgatttaaa aattatggac gcgactcagg
    21541 cgcgggatgt ggtgaagatg aacctgccac gcgtgctgga gaatctgcgt ttgctggtga
    21601 gtgagggtgt caacgtgatc ccgcgtttac cgttgatccc tggtttcacg ctcagcaggg
    21661 agaatatgca gcaggcgctg gatgtactga tcccgctgaa aatcaggcag atccatctgt
    21721 taccgtttca tcagtacggc gaaccgaaat accgcctgct ggggaaaaca tggtcgatga
    21781 aagaggtgcc tgcgccgtcg tcagccgatg tgacaacgat gcgcgaaatg gcagaacggg
    21841 ccggatttca ggttaccgtg ggaggttaaa atggcatacc tggtggcagt aaccgcctgc
    21901 gtaagcggcg tggcgcatac ttatatggcg gcggaacggc tggaaaagtt gtgccagtta
    21961 gagaagtggg gcgtcagcat tgaaactcag ggcgcgctgg gaacggagaa tcgtttagcg
    22021 gacgaggata tccgtcgggc ggatgttgct ctgttgatta cggatatcga gcttgccggt
    22081 gccgagcgat ttgaacattg ccgctatgtg caatgcagca tctacgcatt cctgcgtgag
    22141 ccgcagcggg taatgagcgc ggtgcgcaaa gtgctttctg cgccgcagca aacccatctt
    22201 attctggagt agtcggtttt tctgtcagtt ggctgtggta ctgccggcga tattccgacg
    22261 gcgagcgttc ggtgttttta cgaaacagac ggcagaagta gttgctgtcg acaaagccgc
    22321 aggcgtgcgc cacttctttt accttcaggt catagccttt caataacgtc ttagcgtgct
    22381 ccagtcgcgt gtgattcagg tattcgttaa aaccaatggc ccccgttttt tgaaacaggt
    22441 gcgagagata gtttggtgaa atatagaatg cctgtgcgac agattcgcgg gtaagcgcgg
    22501 aggcatagcg ttcgtcgata taatcgcgaa tagcttcaaa tagtgcctgg ctgcgtgagg
    22561 cggtctggat ttggctgccg agcaaatcgc ggcagtggct aagcaggctg gctacgataa
    22621 gccttgccgt ttgctgctcc tgcggctgca tttgcatttc attgagcgtt tgtagcagaa
    22681 aagaacctat acgtgggccg cgtcgggcga cgtgttgctt cgccagattt tgatattgtt
    22741 tgccatccca ttgcacgacg ctgaagccga gttgctgttt gccaaacagc acgctaaagg
    22801 tggtagcggg tgttttccat tgtgggaaat tccagccacc agcggcaacg tacagcacat
    22861 cgccgggaac taacgttgct ccagcgccgg tatcaacaaa ctcaccttcc agcacaattt
    22921 ccagccgtgg aaaatcgacc tgatacgcaa gatcgggcac cgggccgtta ctgctggcaa
    22981 agtaaatctg gcgcagggac agcgggccgt tgatcaggcg ggagagcaga tagctgacgc
    23041 cgtgatacat gttattccct taggtatgta gacgggaaaa cagtaactgg cattattaaa
    23101 aaaggccacc tgaaaagtgt ggcctgaggg gagttcgatg taaacattgc ggcagttgta
    23161 ttgccggatg tggcgtaaac gccttatcca gcctaccgcc gcgatctgta ggccggataa
    23221 gacgcgtcaa gcgtcgcatc cggcaaatag cgcctggctg ataattactg attacctacc
    23281 tggtcgccat acggcagagt gtcgtaatcg atcagcgcgt ttttcttgta cgggttacca
    23341 atccagcggg tagtttcttt gaactgcgga ttcaccactg aacgggttgg atcgtaaccg
    23401 tcgtaagata agcccgccaa atctgaccag gtgtggatca gttccgccag gctgtattta
    23461 cgatcgacat cctgtgagaa atcacgggga tgagtcgctt gccatttttc cgacgtccac
    23521 agcaggaacg gaatggtgta catgtggcgc gtcgggttgt cttcgttacg cccctgagtt
    23581 ttgtgcggcg gcgtgtcgta aacctcttca ccgtggtcag agaagtaaac caggaatccg
    23641 ttcgggtctg ccgctttaaa gtctttaatc aggctggcaa ccacatgatc gttatacagg
    23701 ttagcgttgt cataatcgtt atatgattcc agctcttctg cgcttaatcc tggcggaaca
    23761 tgatcggtat tgccatcaaa cttgccctga ttttccgggt agcggtattt gtatttgata
    23821 tgcgtaccca gcagatgaac gatgatcagt ttcttcggcg cagggtcatt cagcacatcc
    23881 tggaacggct tcagcacgtt ggtgtcgtat tcacgcgcac tctgcgtacg ttgctggttc
    23941 atgtagtact gcttgtcggt ctggcgcgaa aataccgtca gcatggtatt gcgggcggtc
    24001 atcgtctgct ggttggtgat ccagaaggtt ttataacccg cctgtttcat catgttcatc
    24061 agcgacggct gcgtcagata cagatccggg ttcttttcat tggcaaaggt cagcgcctgt
    24121 tgcaggattt caatggtgta cggacgagac gtaactacgt tattaaacac ggtcagattc
    24181 ggatcggttt tatgcagcgc atccagctcc ggcgtggttt cacgcggata accgtacaga
    24241 ctcatgcgtc cgcgctgggt cgactcgcca atcaccagca ctaaagtgcg cggttcgtta
    24301 cccgattcat ctttgaaatt agccagtggc ggcaaggcat tattttcatt cagtaacttt
    24361 gttagcgagt ttagttgctg acgatactga taatagccgg tcaggaattg ccacggtgcg
    24421 gcaggctcca tacgcgaggc caggttatcc aacgttttct caaacggctt gtttttgata
    24481 aacgtattca tggcgatcgg atgcagaatc aagccataaa gcagggcaaa agagacaaca
    24541 taacgccacg gctttggaat atagaccggg cgcaggcgtg tccacagcag aactgccacc
    24601 gccgtatagg ccagcgcgat aagcacaatt ttcaggctga aatactggct taaatactcg
    24661 ctggcttcgt tggtgttggt ttcgaacatc acaaacagaa cgctctgcga gaactcctgc
    24721 ccgtagatga cgtagtagca tagcgccgcc agagaggccg cccatagcac cacgccgatt
    24781 actgcggcaa taattttaat ccgcttcgga aagaggaata ccgggatcaa ccacagcgag
    24841 ctgaataaca gcgagtcgcg aatgccgttg gtgccactat aaccactgat gtaaataatg
    24901 gcctgtagca gagtagagaa aaaccaaaag tagagcagtg cccaacccag ggctttccag
    24961 ctaaaaaggg gtttagcctg gacttctgtg gaatgcatag taagaacctg tcttgaaaaa
    25021 aatatcgccg aatgtaacga caattcctta aggatatctg aaggtatatt cagaatttga
    25081 ataaaatgca gacagaaata tattgaaaac gagggtgtta gaacagaagt atttcagaaa
    25141 accctcgcgc aaaagcacga gggtttgcag aagaggaaga ttagccggta ttacgcatac
    25201 ctgccgcaat cccggcaata gtgaccatta acgcttgttc gacgcgagga tccggttcct
    25261 ggccttcttt ttctgcctgg cgggagcggt gcagcaactc ggcctgcaat acgttcagcg
    25321 ggtcggtgta aatattccgt agctgaatag actctgcaat ccacggcaga tcggccatca
    25381 gatgggaatc gttggcaatc gccagcacca ctttgatgtc ttcttcttgc aggttgcgta
    25441 actctttacc taacggccac agtgctttgt ctaccagacg ttgatcatag tattccgcca
    25501 accacaggtc tgctttggcg aagaccatct ccagcatgcc gagacgcgtc gagaagaatg
    25561 gccaatcgcg gcacatggct tccagctcgc tctgtttgcc atcttcgacc actttttgca
    25621 gcgccgtacc tgcacccagc caggcgggga gcatcaggcg gttttgcgtc caggcaaaaa
    25681 tccacggtat ggcgcgcagc gactcgacgc cgccggttgg gcgacgtttc gccggacgtg
    25741 aacccaacgg cagtttgccc aattcttgtt ccggcgtagc ggagcggaag taaggcacaa
    25801 aatctttgtt ttcacgtacg tagccacggt agagatcgca ggagatgact gacagttcat
    25861 ccataatgcg acgccagctc tctttcggct caggtggtgg cagcaggttg gcttccagaa
    25921 tcgccccggt gtaaagcgac aggctgctga cggtgatttc tggcagacca tatttaaagc
    25981 ggatcatctc gccctgttca gtcacgcgca ggccaccttt caggcttcct ggcggttgtg
    26041 acagcagcgc cgcatgggca ggtgcgccgc cgcgaccaat ggaaccgccg cgaccgtgga
    26101 acaacgtcag ctcaataccc gctttttcgc aggttttgat taatgcatcc tgtgcctgat
    26161 attgcgccca ggaagctgcc atcacgcccg catcttttgc tgagtcggaa tagccaatca
    26221 tcaccatctg tttgccctga atcaggccgc gataccagtc gatattgagc agctgggtca
    26281 tgacatcgtt ggcgttgttc aggtcatcga gggtttcaaa cagcggagca accggcatcg
    26341 caaacccgat acccgcttct ttcagcagca ggtggacagc cagtacgtcg gacggcgttt
    26401 tcgccatcga gatcacgtag gcggcaatgg agccttgcgg cgcttcggca atcacctggc
    26461 aggtatcgag cacttcgcgc gtttcggcgc ttggttgcca gttgcgcggc agaagcggac
    26521 gtttggagtt cagttcgcgg atcaggaacg cctgtttgtc ggcctctgac cagctttcgt
    26581 agtcgccgat accgaggtag cgggtcagct cgcccagcgc ttcggtatgg cgggtgctct
    26641 cctgacggat atcaatacgg accagcggta cgccgaaaca tttcacgcgg cgcagggtgt
    26701 cgagcagatc gccgttggcg ataataccca tgccacacgc ctgaagtgac tggtagcaag
    26761 cgtagagcgg ttcccacagt tcttcgtttt gtgtcagcag cccttctggt tttggcagtt
    26821 cttcgccttt caggcgcgct tccagccatg cctgtgtcgc catcaggcga gaacgcaggt
    26881 ttttcatcag atagcgatac ggttctgcgg caccttcttc gccaaccagc gccagcagtt
    26941 caggggtcgc ttcaaccatc gacagttcag aaaccagaac ctgaatatct ttcaggaaca
    27001 gatcggtggc tttccagcga ctgagtagca ggacgtggcg ggtgatatcg gcagtgacgt
    27061 tcgggttgcc gtcgcggtca ccgcccatcc acgaagtaaa acggaccgga acaaattcga
    27121 cgggcagttt gtagccgagg ttctcttcca gttgttcgtt cagttcgcgc aagtaattcg
    27181 gtacgccttg ccacaggctg ttttctacta cggcaaagcc ccatttggct tcatctaccg
    27241 ggcttggacg cagcttacgg atttcatcag tatgccatga ctgggcgatt aactggcgca
    27301 ggcgacgcat cagctggttg tgttcgtagt cagcgatatc tttgttatcg agctgtttta
    27361 agcaggcgtt cacttccacc attttgtgga tcagtgtacg acgggtaatt tcggttgggt
    27421 gagccgtgag gaccagttcc agcgacagcg attccactgc ttttttgatg gtgtcttcgc
    27481 tcagttccgg ctggtttttc agtttgcgca gggtgcgggc gatcacttcc gggttgctgg
    27541 cagcttcgcc tttcggcgaa atgctgtggt attgctcggc ggtgttggcc aggttcagga
    27601 actgactaaa cgcacgcgca acgggcagca gctcgtcgtt cgacaaattt tgtaaggtgg
    27661 tgagcaactc ctggcggtta gcatcattgc cagcgcgtga agatttcgac aacttacgga
    27721 tagtttctac gcgttcaaga atgtgttctc ccaacgcatc cttgatggtt tctcccagca
    27781 ctttgccgag catactgaca ttactacgca atgcggaata ttgttcgttc atattacccc
    27841 agacacccca tcttatcgtt tgatagccct gtatccttca cgtcgcattg gcgcgaatat
    27901 gctcgggctt tgcttttcgt cgtcttttat aaagccacgt aaaagcggtg acgtcaaatg
    27961 ctgcgaaatc gcttcagcaa acgaataaat agcaggaatt tacgccatta aattcacgac
    28021 gctttaaata agcgtaactt atggaaatgt taaaaaatcg ccatacataa caccaaaggt
    28081 gtaggtcgga taagatgcgc aagcatcgca tccgacatta ttgcggcacc ggagtttggc
    28141 aacggtgccg gatgcggcgc gagcgcctta tccggcctac gttttaatgc cagcaaaaat
    28201 ggtgaattac ctggataatc aactcacggg tgggtttaat aaaccgcgtc tccagatatt
    28261 catcaggctg atgagcttga ttgatggaac ccggaccgag caccagcgtc gggcataacg
    28321 tttgaataaa cggcgcttcg gtacagtaat tcaccacttc ggtttttgct ccgagcaatt
    28381 tctcaaccac ttcaaccagt tgatgattcg gtgggcattc atagccaggg atcggcggat
    28441 gcagctcgtc gaccgtcaga cgacccggcc agcgttcgct caccggagcc aatgcatcgt
    28501 tgagcaaacc attaagttca ttgagtgtca tgctaggcag cggacgaata tccatatgca
    28561 actcacagca agcgcaaata cggttagaag cgtcgccacc gtgaatatgc ccgaggttga
    28621 gcgtagggta tggcacggta aatgcttcgt agtgataacg ttctttcagg ttatcgcgca
    28681 attgcaaaat atgcccgatg gcgtcgtgca ttagttcgat agcgttaact ccgcgtgctg
    28741 gatcgctgga gtgccccgac tggccctgaa tacggatggc gttagagata tgacctttat
    28801 gtgcgcgtac cggttgtagt gacgtcggtt cgccaatgat ggcgcaatcc gggcgcaggg
    28861 cggtagtttc ggcaaaataa cgcgctccgg ccatactggt ttcttcatca gcagtcgcca
    28921 gaatgtagag cggttttttc agtttcgtga cgtcgacatc gcgtagcgta tcaaggataa
    28981 acgcaaaaaa gcctttcatg tcggcggtgc ctaagccgta aagcttgccg tcatgctccg
    29041 tcagtgtaaa cggatcgcgc gtccagcgac cgtcatcaaa tggcaccgta tcggtatgcc
    29101 ccgccagcaa caagccgcca gccccctgtc cgatacttgc cagcatattg aatttgttgc
    29161 gagttcctgg cacaggctgc acttccacat tgaagcctaa atctttaaac cagtccgcca
    29221 gcagagtgat taaatctgca ttgctttgat cgagtgcctc ttccgtggcg cttattgaag
    29281 gtgtggcaat cagagcacgg taaatctcga taaatggcgg taatttgttt ttcattgttg
    29341 acacacctct ggtcatgata gtatcaatat tcatgcagta tttatgaata aaaatacact
    29401 aacgttgagc gtaataaaac ccaccagccg taaggtgaat gttttacgtt taacctggca
    29461 accagacata agaaggtgaa tagccccgat gttgaatacg ctgattgtgg gtgccagcgg
    29521 ctacgctggc gcagagctag tgacctatgt aaatcgccat ccgcatatga acataaccgc
    29581 tttgactgtt tcagcgcaaa gcaatgatgc gggaaagtta atctccgatt tgcatccgca
    29641 gctaaaaggc atcgttgagc tgccgttgca gccgatgtcg gatatcagcg agtttagccc
    29701 aggggtggac gtagtgtttc tcgccaccgc ccatgaagtt agccacgatt tagcgccgca
    29761 gtttcttgaa gcgggctgcg tggtgttcga cctttccggt gcgtttcgtg ttaacgacgc
    29821 caccttctat gaaaaatatt acggctttac ccatcaatac ccggaactgc tggaacaggc
    29881 agcctacggt ctggcggagt ggtgcggtaa taaattaaaa gaagcgaatt tgattgcggt
    29941 gccaggctgt tatccgacgg cggcacagct ggcgctgaaa ccgttgattg atgccgatct
    30001 tcttgacctc aatcagtggc cggtgatcaa cgccaccagc ggcgtgagcg gtgcaggacg
    30061 taaggcagcg atttcaaata gtttttgtga agttagcctg caaccgtatg gcgtctttac
    30121 tcatcgccat caaccagaga tcgccacaca cctcggtgct gacgttatct tcaccccaca
    30181 tctgggcaat ttcccgcgcg gcattctcga aaccattacc tgccgcctga aatcgggtgt
    30241 gacccaggcg caagtcgcgc aagtgttaca gcaggcgtat gcccataaac cgctggtgcg
    30301 gctgtatgac aaaggcgttc cggcgctgaa aaatgtcgtt gggctgccat tttgcgatat
    30361 cgggtttgcc gttcagggcg agcatctgat tattgtggcg accgaagaca acttactgaa
    30421 aggcgcggcg gcacaagcgg tacagtgcgc caatattcgt ttcggctatg cggaaacgca
    30481 gtctcttatt taagggtgca atgatgaatc cattaattat caaactgggc ggcgtactgc
    30541 tggatagtga agaggcgctg gaacgtctgt ttagcacact ggtgaattat cgtgagtcac
    30601 atcagcgtcc gctggtgatt gtgcacggcg gcggttgcgt ggtggatgag ctgatgaaag
    30661 ggctgaatct gccggtgaaa aagaaaaacg gcctgcgggt gacgcctgct gatcagatag
    30721 acattatcac cggagcactg gcgggaacgg caaataaaac cctgttggca tgggcgaaga
    30781 aacatcagat tgcggccgta ggtttgtttc tcggtgacgg cgacagcgtc aaagtgaccc
    30841 agcttgatga agagttaggt catgttggac tggcgcagcc aggttcgcct aagcttatca
    30901 actccttgct ggagaacggt tatctgccgg tggtcagctc cattggcgta acagacgaag
    30961 ggcaactgat gaacgtcaat gccgaccagg cggcaacggc gctggcggca acgctgggcg
    31021 cggatctgat tttgctctcc gacgtcagcg gcattctcga cggcaaaggg caacgcattg
    31081 ccgaaatgac cgccgcgaaa gcagaacaac tgattgagca gggcattatt actgacggca
    31141 tgatagtgaa agtgaacgcg gcgctggatg cggcccgcac gctgggccgt ccggtagata
    31201 tcgcctcctg gcgtcatgcg gagcagcttc cggcactgtt taacggtatg ccgatgggta
    31261 cgcggatttt agcttaagtt ttgttggccg gaggcgcagc tttccggcat tgaatttcaa
    31321 aataaggaaa cagagttatg gcactttggg gcgggcgttt tacccaggca gcagatcaac
    31381 ggttcaaaca attcaacgac tcactgcgct ttgattaccg tctggcggag caggatattg
    31441 ttggctctgt ggcctggtcc aaagccctgg tcacggtagg cgtgttaacc gcagaagagc
    31501 aggcgcaact ggaagaggcg ctgagcgtgc tgctggaaga tgttcgcgcc aggccacaac
    31561 aaatccttga aagcgacgcc gaagatatcc atagctgggt ggaaggcaaa ctgatcgaca
    31621 aagtgggcca gttaggcaaa aagctgcata ccgggcgtag tcgtaacgat caggtagcga
    31681 ctgacctgaa actgtggtgc aaagataccg ttagcgagtt actgacggct aaccgacagc
    31741 tgcaatcggc gctggtggaa accgcacaaa acaatcagga cgcggtcatg ccaggttaca
    31801 ctcacctgca acgcgcccag ccggtgacgt tcgcgcactg gtgcctggcc tatgttgaga
    31861 tgctggcgcg tgatgaaagc cgtttgcagg atgcgcttaa gcgtctggat gtcagcccat
    31921 taggctgtgg cgcgctggcg ggaacggcct atgaaatcga ccgtgaacag ttagcaggct
    31981 ggctgggctt tgcttcggcg acccgtaaca gtctggacag cgtttctgac cgtgaccatg
    32041 tgttggaact gctgtcggct gccgctatcg gcatggtgca tctgtcgcgt tttgctgaag
    32101 atctgatttt ctttaacacc ggcgaagcgg ggtttgtgga gctttctgac cgcgtgactt
    32161 ccggttcatc attaatgccg cagaagaaaa acccggatgc gctggagctg attcgcggta
    32221 aatgcggtcg ggtgcagggt gcgttaaccg gcatgatgat gacgctgaaa ggtttgccgc
    32281 tggcttacaa caaagatatg caggaagaca aagaaggtct gttcgacgcg ctcgatacct
    32341 ggctggactg cctgcatatg gcggcgctgg tgctggacgg cattcaggtg aaacgtccac
    32401 gttgccagga agcggctcag cagggttacg ccaacgccac cgaactggcg gattacctgg
    32461 tggcgaaagg cgtacctttc cgcgaggcgc accatatcgt gggtgaagcg gtggtggaag
    32521 ccattcgtca gggcaaaccg ctggaagatc tgccgctcga cgagttgcag aaattcagcc
    32581 cggtgattgg cgaagatgtc tatccgattt tgtcgctgca atcgtgcctc gacaagcgtg
    32641 cggcaaaagg cggcgtctca ccgcagcagg tggcccaagc gattgctttt gcgcaggcgc
    32701 ggttggggta agaacattta tatgtacaaa tttgagcctg gcttatcgcc gggctttttt
    32761 atggcaaaaa aaagcggatc ctggagatcc gcaaaagttc acgttggctt tagttattcg
    32821 agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt
    32881 tctgtgagca attatcagtc agaatgcttg atagggataa tcgttcattg ctattctacc
    32941 tatcgccatg aactatcgtg gcgatggagg atggataatg aatattcgtg atcttgagta
    33001 cctggtggca ttggctgaac accgccattt tcggcgtgcg gcagattcct gccacgttag
    33061 ccagccgacg cttagcgggc aaattcgtaa gctggaagat gagctgggcg tgatgttgct
    33121 ggagcggacc agccgtaaag tgttgttcac ccaggcggga atgctgctgg tggatcaggc
    33181 gcgtaccgtg ctgcgtgagg tgaaagtcct taaagagatg gcaagccagc agggcgagac
    33241 gatgtccgga ccgctgcata ttggtttgat tcccacggtt ggaccgtacc tgctaccgca
    33301 tattatcccg atgctgcacc agacctttcc aaagctggaa atgtatctgc atgaagcaca
    33361 gacccaccag ttactggcgc aactggacag tggcaaactc gattgcgtga tcctcgcgct
    33421 ggtgaaagag agcgaagcat tcattgaagt gccgttgttt gatgagccaa tgttgctggc
    33481 tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta ccgatggccg atctggcagg
    33541 ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg
    33601 ttttgaagcc ggggcggatg aagatacaca cttccgcgcg accagcctgg agacgctgcg
    33661 caacatggtg gcggcaggta gcgggatcac tttactgcca gcgctggctg tgccgccgga
    33721 gcgcaaacgc gatggggttg tttatctgcc gtgcattaag ccggaaccac gccgcactat
    33781 tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc tatgagcagc tggcagaggc
    33841 catccgagca agaatggatg gctatttcga taaagtgtta aaacaggcgg tttaaaccgt
    33901 ttaacgcagc tacccgatag gcttccgcca tggtcgggta gttaaaggtg gtgttgacga
    33961 agtactcaat agtgttgccg ccacctttct gttccataat cgcctgaccg atatgaataa
    34021 tttcggcagc gcgctcgcca aagcagtgaa tacccagaat ctcttttgtt tcccgatgga
    34081 acaaaatttt cagcgtgccc acgttcatgc cgacgatttg tgcgcgtgcc agatgtttaa
    34141 actgggcgcg gcccacttca tatggcactt tcatcgcggt cagctgctgt tcggttttgc
    34201 ccacagagct gatttccggg atggtgtaaa tgccggtagg gatatcttca atcagatgcg
    34261 cggtggcttc gccttttacc agcgcctgcg cggcaatgcg cccctggtca taggccgctg
    34321 atgccaggct cggataacca atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct
    34381 gatacatgct gttgaccttc agctgtccgc gactgtcagt ttccagccca atgttctgta
    34441 acgccagcga atcggtatta ccggtacgac cgttggcata gagcaggcag tcagctttca
    34501 gttttttacc cgacttcaga tgcatgatca caccgtcgtc acagccttcg atcttctcgt
    34561 actcttcgtt gtgacgaatc actacgccac tgttccagaa gtgataggag agagaatctg
    34621 acatctcttg atcgagaaat gccagcaggc gatcgcgggt gttgatcaga tccactttta
    34681 catccatacc gcggaagatc gacgcatatt cacagccgat cactccagca ccatagataa
    34741 gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc gctgtcgtaa atgcgtggat
    34801 gggtgaaatc aacatctgtt ggatgatatg gacgagagcc gcaggcaata acaaattttt
    34861 cagcggttag tgtttcgacg ctgccgtccg ggcaatccag cgccaacgta tgctcgtcaa
    34921 caaagcgagc gttcccctgc aatatttcac agtgattacg ttcgtaaaat ccctgacgca
    34981 tgcgcgtttg ttgattaatc acgttatcgg catggttaag gatatcggca aaagaagagc
    35041 ggagcagtcg cgaatggtcg ctgtatagtg ggttttgatt gaattctata atgcggctga
    35101 cggcgtgacg gagagctttc gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat
    35161 tttgataacg ctcgataact gcgacgcgca caccttgctt aaccaggccc attgcagcgc
    35221 cttcgccgcc ggggccggaa cctattacta tggcatcgta atcgtaggaa tgtggcatgg
    35281 tagggcttac ctgttcttat acataaaagc aacagaatgg taacattcta tcgcgggtaa
    35341 gccaattgat ccccgtcatt tatctggcta tatcctgagc ggcctttgct ttgtctgttt
    35401 cttacttttg ccctgacgtt ttattggatt tttatcgacg atactctccg tttaagcggc
    35461 aggtttccgc tgtacgtaaa agaaccggcc agtaaatatg ttttattgcg ttaccgttca
    35521 ttcacaatac tggagcaatc cagtatgttc attctctggt atagtgccag cagtactttt
    35581 ggcaaggatt cagacatcgt gatgggcata agagcgcaac aaaaagaaaa aacccgccgt
    35641 tcgctggtgg aagccgcatt tagccaatta agtgctgaac gcagcttcgc cagcctgagt
    35701 ttgcgtgaag tggcgcgtga agcgggcatt gctcccacct ctttttatcg gcatttccgc
    35761 gacgtagacg aactgggtct gaccatggtt gatgagagcg gtttaatgct acgccaactc
    35821 atgcgccagg cgcgtcagcg tatcgccaaa ggcgggagtg tgatccgcac ctcggtctcc
    35881 acatttatgg agttcatcgg taataatcct aacgccttcc ggttattatt gcgggaacgc
    35941 tccggcacct ccgctgcgtt tcgtgccgcc gttgcgcgtg aaattcagca cttcattgcg
    36001 gaacttgcgg actatctgga actcgaaaac catatgccgc gtgcgtttac tgaagcgcaa
    36061 gccgaagcaa tggtgacaat tgtcttcagt gcgggtgccg aggcgttgga cgtcggcgtc
    36121 gaacaacgtc ggcaattaga agagcgactg gtactgcaac tgcgaatgat ttcgaaaggg
    36181 gcttattact ggtatcgccg tgaacaagag aaaaccgcaa ttattccggg aaatgtgaag
    36241 gacgagtaat gaaacaagca aatcaagata gaggtacgct gctgctggcg ttagttgctg
    36301 gcttatcgat taatggtact ttcgcagcgc tgtttagctc cattgtgcca ttttctgtat
    36361 tcccgattat ttccctggtg ctgacggttt actgcctgca tcaacgttat cttaatcgca
    36421 ctatgccggt aggcttgccg ggtctggcag ctgcctgttt tattctcggc gtactgctgt
    36481 acagcacggt agttcgtgcg gaatatccgg atatcggctc taacttcttc ccggcagtac
    36541 tctccgtcat tatggtgttc tggattggcg cgaagatgcg taaccgtaag caggaagttg
    36601 ctgagtaatc ggtattatgc cggatgctgg cgcatccggc atgggtttta cttcgcggtc
    36661 agtaatacgc cgcactccat atggtgcgtg taggggaact gatcaaacag agccagacgt
    36721 tcgaccttgt gcgtctggct taatgtttcc agattcttgc ataacgtttc cgggttacag
    36781 gagatgtaca aaatacacgg atacgcctgc accattttct cggtttcact gtccagaccg
    36841 ctgcgcggtg ggtcgacaaa aatggtttcg cactgataac tctttaagtc gatcccttgc
    36901 aggcggttaa actcgcgcac accattcatc gcctgagtaa attcttctgc cgccatacga
    36961 ataatttgta cgttatcaat atggttagct gcgatgttgt attgcgcagc agcaaccgac
    37021 ggcttagcga tttcggtggc taatacccga tcaaaattgc gcgccagcgc taatgaaaag
    37081 ttaccgttgc cgcagtacag ctccagtaaa tcgccttttg agcctttggt tacgtccagc
    37141 gcccattcca gcatctgaat attcatcgcc gcgttcggct gggtaaagct gttttctacc
    37201 tgacggtaga tcatctcttt ccctgcgacc ggcagacgct cgtcgatgta atcttgatcc
    37261 agctcgattt tggttttcgt tgcccgacca atcagatgca cattcagatt ctgcgcgcgc
    37321 agtgcatcgc gcagggcctc cgcttcctga cgccactcat catccagctt cttatggtat
    37381 agcagggaaa ccaccgcctg attactcagc gtggtgaggt aatcaatctg gaacaacttg
    37441 tggcgcagaa cgggattatt acgcacaccc gcaatcatcg ccgtcatcaa ctggttgata
    37501 agttcactgg cggcggggaa gctatccacg cggatacggc ttttggtttg ttgatcgaaa
    37561 atgatgtgat acaggtcatc gccatcgtgc cagatgcgga actccgcacg catccggtaa
    37621 tgactgaccg gcgagcgaaa cacttccgga accaggtcag aaaacggtgc catcatactt
    37681 tgcaaacgta ccactttttc ggctaactgc gcttcatact gttctgttgg aaggtgttcg
    37741 ggggtcatga tgtatcctga aaaattaaag tacgcggcga ttgtagggat tgctcatcag
    37801 atgtccagat cttgatgaat tcctatttgt gagctacgtc tggacagtaa cttgttacaa
    37861 cctgtagcat ccacttgtcg gtcctgtgag ttaataggga atccagtgcg aatctggagc
    37921 tgacgcgcag cggtaaggaa aggtgcgatg attgcgttat gcggacactg ccatccggtg
    37981 ggaagtcatc atctcttagt atcttagata cccctccaag cccgaagacc tgccggccaa
    38041 cgtcgcatct ggttctcatc atcgcgtaat attgatgaaa cctgcggcat ccttcttcta
    38101 ttgtggatgc tttacaatga ttaaaaaagc ttcgctgctg acggcgtgtt ctgtcacagc
    38161 cttttccgct tgggcacagg ataccagccc ggatactctc gtcgttactg ctaaccgttt
    38221 tgaacagccg cgcagcactg tgcttgcacc aaccaccgtt gtgacccgtc aggatatcga
    38281 ccgctggcag tcgacctcgg tcaatgatgt gctgcgccgt cttccgggcg tcgatatcac
    38341 ccaaaacggc ggttcaggtc agctctcatc tatttttatt cgcggtacaa atgccagtca
    38401 tgtgttggtg ttaattgatg gcgtacgcct gaatctggcg ggggtgagtg gttctgccga
    38461 ccttagccag ttccctattg cgcttgtcca gcgtgttgaa tatatccgtg ggccgcgctc
    38521 cgctgtttat ggttccgatg caataggcgg ggtggtgaat atcatcacga cgcgcgatga
    38581 acccggaacg gaaatttcag cagggtgggg aagcaatagt tatcagaact atgatgtctc
    38641 tacgcagcaa caactggggg ataagacacg gggaacgctg ttgggcgatt atgcccatac
    38701 tcatggttat gatgttgttg cctatggtaa tacaggaacg caagcgcaga cagataacga
    38761 tggtttttta agtaaaacgc tttatggcgc gctggagcat aactttactg atgcctggag
    38821 cggctttgtg cgcggctatg gctatgataa ccgtaccaat tatgacgcgt attattctcc
    38881 cggttcaccg ttgctcgata cccgtaaact ctatagccaa agttgggacg ccgggctgcg
    38941 ctataacggc gaactgatta aatcacaact cattaccagc tatagccata gcaaagatta
    39001 caactacgat ccccattatg gtcgttatga ttcgtcggcg acgctcgatg agatgaagca
    39061 atacaccgtc cagtgggcaa acaatgtcat cgttggtcac ggtagtattg gtgcgggtgt
    39121 cgactggcag aaacagacta cgacgccggg tacaggttat gttgaggatg gatatgatca
    39181 acgtaatacc ggcatctatc tgaccgggct gcaacaagtc ggcgatttta cctttgaagg
    39241 cgcagcacgc agtgacgata actcacagtt tggtcgtcat ggaacctggc aaaccagcgc
    39301 cggttgggaa ttcatcgaag gttatcgctt cattgcttcc tacgggacat cttataaggc
    39361 accaaatctg gggcaactgt atggcttcta cggaaatccg aatctggacc cggagaaaag
    39421 caaacagtgg gaaggcgcgt ttgaaggctt aaccgctggg gtgaactggc gtatttccgg
    39481 atatcgtaac gatgtcagtg acttgatcga ttatgatgat cacaccctga aatattacaa
    39541 cgaagggaaa gcgcggatta agggcgtcga ggcgaccgcc aattttgata ccggaccact
    39601 gacgcatact gtgagttatg attatgtcga tgcgcgcaat gcgattaccg acacgccgtt
    39661 gttacgccgt gctaaacagc aggtgaaata ccagctcgac tggcagttgt atgacttcga
    39721 ctggggtatt acttatcagt atttaggcac tcgctatgat aaggattact catcttatcc
    39781 ttatcaaacc gttaaaatgg gcggtgtgag cttgtgggat cttgcggttg cgtatccggt
    39841 cacctctcac ctgacagttc gtggtaaaat agccaacctg ttcgacaaag attatgagac
    39901 agtctatggc taccaaactg caggacggga atacaccttg tctggcagct acaccttctg
    39961 aaccacgtcc caccgtgctg gtgtttgact ccggcgtcgg tgggttgtcg gtctatgacg
    40021 agatccggca tctcttaccg gatctccatt acatttatgc tttcgataac gtcgctttcc
    40081 cgtatggcga aaaaagcgaa gcgtttattg ttgagcgagt ggtggcaatt gtcaccgcgg
    40141 tgcaagaacg ttatcccctt gcgctggctg tggtcgcttg caacactgcc agtaccgttt
    40201 cacttcctgc attacgcgaa aagttcgact tcccggttgt tggtgtcgtg ccggcgatta
    40261 aacctgctgc acgtctgacg gcaaatggca ttgtcggatt actggcaacc cgcggaacag
    40321 ttaaacgttc ttatactcat gagctgatcg cgcgtttcgc taatgaatgc cagatagaaa
    40381 tgctgggctc ggcagagatg gttgagttgg ctgaagcgaa gctacatggc gaagatgttt
    40441 ctctggatgc actaaaacgt atcctacgcc cgtggttaag aatgaaagag ccgccagata
    40501 ccgttgtatt gggttgcacc catttccctc tactacaaga agaactgtta caagtgctgc
    40561 cagagggaac ccggctggtg gattctggcg cagcgattgc tcgccgaacg gcctggttgt
    40621 tagaacatga agccccggat gcaaaatctg ccgatgcgaa tattgccttt tgtatggcaa
    40681 tgacgccaga agctgaacaa ttattgcccg ttttacagcg ttacggcttc gaaacgctcg
    40741 aaaaactggc agttttaggc tgatttggtt gaatgttgcg cggtcagaaa attattttaa
    40801 atttcctctt gtcaggccgg aataactccc tataatgcgc caccactgac acggaacaac
    40861 ggcaaacacg ccgccgggtc agcggggttc tcctgagaat ctcaacagag aaaagcaaaa
    40921 aaatgcttga ctctgt
//
LOCUS       DAJTVK010000022        39775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000022 DAJTVK010000000
VERSION     DAJTVK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.022"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..108)
                     /locus_tag="OO372_001246"
     tRNA            complement(33..108)
                     /locus_tag="OO372_001246"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(73..75),aa:Lys,seq:ttt)
     gene            complement(157..232)
                     /locus_tag="OO372_001247"
     tRNA            complement(157..232)
                     /locus_tag="OO372_001247"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Lys,seq:ttt)
     gene            complement(236..311)
                     /locus_tag="OO372_001248"
     tRNA            complement(236..311)
                     /locus_tag="OO372_001248"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(276..278),aa:Val,seq:tac)
     gene            complement(461..536)
                     /locus_tag="OO372_001249"
     tRNA            complement(461..536)
                     /locus_tag="OO372_001249"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Lys,seq:ttt)
     gene            complement(701..1492)
                     /gene="cpoB"
                     /locus_tag="OO372_001250"
     CDS             complement(701..1492)
                     /gene="cpoB"
                     /locus_tag="OO372_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6340797.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1502..2023)
                     /gene="pal"
                     /locus_tag="OO372_001251"
     CDS             complement(1502..2023)
                     /gene="pal"
                     /locus_tag="OO372_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6340798.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2058..3350)
                     /gene="tolB"
                     /locus_tag="OO372_001252"
     CDS             complement(2058..3350)
                     /gene="tolB"
                     /locus_tag="OO372_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6340799.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3483..4715)
                     /gene="tolA"
                     /locus_tag="OO372_001253"
     CDS             complement(3483..4715)
                     /gene="tolA"
                     /locus_tag="OO372_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6340800.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4780..5208)
                     /gene="tolR"
                     /locus_tag="OO372_001254"
     CDS             complement(4780..5208)
                     /gene="tolR"
                     /locus_tag="OO372_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6340801.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5212..5904)
                     /gene="tolQ"
                     /locus_tag="OO372_001255"
     CDS             complement(5212..5904)
                     /gene="tolQ"
                     /locus_tag="OO372_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6340802.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5901..6305)
                     /gene="ybgC"
                     /locus_tag="OO372_001256"
     CDS             complement(5901..6305)
                     /gene="ybgC"
                     /locus_tag="OO372_001256"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6340803.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYVPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            complement(6455..6748)
                     /gene="ybgE"
                     /locus_tag="OO372_001257"
     CDS             complement(6455..6748)
                     /gene="ybgE"
                     /locus_tag="OO372_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6340804.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6748..6861)
                     /gene="cydX"
                     /locus_tag="OO372_001258"
     CDS             complement(6748..6861)
                     /gene="cydX"
                     /locus_tag="OO372_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6340805.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6876..8015)
                     /gene="cydB"
                     /locus_tag="OO372_001259"
     CDS             complement(6876..8015)
                     /gene="cydB"
                     /locus_tag="OO372_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6340806.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8031..9599)
                     /gene="cydA"
                     /locus_tag="OO372_001260"
     CDS             complement(8031..9599)
                     /gene="cydA"
                     /locus_tag="OO372_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6340807.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10447..13003)
                     /locus_tag="OO372_001261"
                     /pseudo
     CDS             complement(10447..13003)
                     /locus_tag="OO372_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(13021..14937)
                     /gene="mngA"
                     /locus_tag="OO372_001262"
     CDS             complement(13021..14937)
                     /gene="mngA"
                     /locus_tag="OO372_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6340808.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            15106..15828
                     /gene="mngR"
                     /locus_tag="OO372_001263"
     CDS             15106..15828
                     /gene="mngR"
                     /locus_tag="OO372_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6340809.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15932..16801)
                     /gene="sucD"
                     /locus_tag="OO372_001264"
     CDS             complement(15932..16801)
                     /gene="sucD"
                     /locus_tag="OO372_001264"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6340810.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16801..17967)
                     /gene="sucC"
                     /locus_tag="OO372_001265"
     CDS             complement(16801..17967)
                     /gene="sucC"
                     /locus_tag="OO372_001265"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6340811.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(18061..19278)
                     /gene="odhB"
                     /locus_tag="OO372_001266"
     CDS             complement(18061..19278)
                     /gene="odhB"
                     /locus_tag="OO372_001266"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6340812.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(19293..22094)
                     /gene="sucA"
                     /locus_tag="OO372_001267"
     CDS             complement(19293..22094)
                     /gene="sucA"
                     /locus_tag="OO372_001267"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6340813.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22395..23111)
                     /gene="sdhB"
                     /locus_tag="OO372_001268"
     CDS             complement(22395..23111)
                     /gene="sdhB"
                     /locus_tag="OO372_001268"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6340814.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(23127..24893)
                     /gene="sdhA"
                     /locus_tag="OO372_001269"
     CDS             complement(23127..24893)
                     /gene="sdhA"
                     /locus_tag="OO372_001269"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6340815.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24893..25240)
                     /gene="sdhD"
                     /locus_tag="OO372_001270"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24893..25240)
                     /gene="sdhD"
                     /locus_tag="OO372_001270"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6340816.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(25234..25638)
                     /gene="sdhC"
                     /locus_tag="OO372_001271"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(25234..25638)
                     /gene="sdhC"
                     /locus_tag="OO372_001271"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6340817.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            26019..26126
                     /locus_tag="OO372_001272"
     CDS             26019..26126
                     /locus_tag="OO372_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6340818.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            26332..27615
                     /gene="gltA"
                     /locus_tag="OO372_001273"
     CDS             26332..27615
                     /gene="gltA"
                     /locus_tag="OO372_001273"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6340819.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            28005..28571
                     /locus_tag="OO372_001274"
     CDS             28005..28571
                     /locus_tag="OO372_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6340820.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28622..31080
                     /locus_tag="OO372_001275"
                     /pseudo
     CDS             28622..31080
                     /locus_tag="OO372_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            31095..31826
                     /locus_tag="OO372_001276"
     CDS             31095..31826
                     /locus_tag="OO372_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6340821.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31823..32884
                     /locus_tag="OO372_001277"
     CDS             31823..32884
                     /locus_tag="OO372_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6340822.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            33036..34082
                     /locus_tag="OO372_001278"
     CDS             33036..34082
                     /locus_tag="OO372_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6340823.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(34079..34870)
                     /gene="nei"
                     /locus_tag="OO372_001279"
     CDS             complement(34079..34870)
                     /gene="nei"
                     /locus_tag="OO372_001279"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6340824.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34906..35640)
                     /gene="pxpA"
                     /locus_tag="OO372_001280"
     CDS             complement(34906..35640)
                     /gene="pxpA"
                     /locus_tag="OO372_001280"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6340825.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(35630..36562)
                     /gene="pxpC"
                     /locus_tag="OO372_001281"
     CDS             complement(35630..36562)
                     /gene="pxpC"
                     /locus_tag="OO372_001281"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR6340826.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(36556..37212)
                     /gene="pxpB"
                     /locus_tag="OO372_001282"
     CDS             complement(36556..37212)
                     /gene="pxpB"
                     /locus_tag="OO372_001282"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6340827.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(37235..37978)
                     /gene="ybgI"
                     /locus_tag="OO372_001283"
     CDS             complement(37235..37978)
                     /gene="ybgI"
                     /locus_tag="OO372_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6340828.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            38249..39730
                     /gene="dtpD"
                     /locus_tag="OO372_001284"
     CDS             38249..39730
                     /gene="dtpD"
                     /locus_tag="OO372_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6340829.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         9637 a        10692 c         9681 g         9765 t
ORIGIN      
        1 ttgcgggatt atgctgcttt aatcattcag gatggtgggt cgtgcaggat tcgaacctgc
       61 gaccaattga ttaaaagtca actgctctac caactgagct aacgacccat acgggtgtta
      121 cctgaaagat ttctactcgg ggccaatctt taagattggt gggtcgtgca ggattcgaac
      181 ctgcgaccaa ttgattaaaa gtcaactgct ctaccaactg agctaacgac ccgagtggtg
      241 ggtgatgacg ggatcgaacc gccgaccccc tccttgtaag ggaggtgctc tcccagctga
      301 gctaatcacc cgatactgcg ctggatacta caaactactt ccaccgaacc caccgtaatt
      361 agtggtgggt cgtgcaggat gactcgcttc gctcgcccta cgggccgttg ctgacgcaac
      421 gttatcctac acgcttaaca tctgtcttcg atgctaaaac tggtgggtcg tgcaggattc
      481 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccactt
      541 tttcgttgct ttcggtttgt ttgatatccc gtggcaacgg cggcatatat tactgatttc
      601 agacttgagc gcaacaaaaa tttcgatgca gatcactcaa ctgcttatga ttcgcacgac
      661 acgaccagaa ataatgcgac ttctggtcgt gtgttatgca ttacatcgcg ttcagacgtt
      721 tttgtgcctg tttagcgcca tcagtaccag ggtatttact gataacctgc tggtatacgg
      781 ctttcgcttt tgcggtgtca cctttgtcct gcatgatgac gccgacttta aacatcgcat
      841 cagcagcctt tggtgacttc ggatagtttt tcactaccga agcaaaatag tacgccgcat
      901 catctttttt acccttgttg tagtttaact gacccagcca ataattggcg tttggcaggt
      961 aagttgaatc agggtaattt ttgatgaaat tctgaaatgc caccattgcg tcatcctggc
     1021 gggatttatc ctgcaccagc gcaatagctg cattgtaatc cgtgtttgca tcgccgcttt
     1081 tcaccggcgc gccagcattc gcagtaccag catcagctgt cggcgtcgtt gatgccgccg
     1141 caccgctttg atcgctgctg gttgattgcg ccgctgcacc accgctgctg aggctgtcga
     1201 tctgcaacag gatctgcttt tgccgctcca cgacctgatt cagttgatac tgattttcct
     1261 gaatctgacc acgcagggaa tcaatatcgg attgattatc agaaagttgt tgctggagtt
     1321 gggttaaaag ctggctgtga gcattagaaa tacgctcaag ttgagtgacg cggtcttcga
     1381 ccgagcctga gccgacacta ctgattggtg cctgagcaaa agcggcccag ggggccgcta
     1441 taccaaccag taacgacaga ctcaatagtt gatgtctgaa gttactgctc atgcaattct
     1501 cttagtaaac cagtaccgca cgacggtttt tggagtatgc cgcttcgtca tgacccagta
     1561 ctgcaggttt ttctttaccg taagaaacga tggagatctg gtctgcagaa acgcctttac
     1621 cctgcaggta catcttaacg gcgttcgcac gacgttcacc cagggagatg ttgtattccg
     1681 gagtaccacg ttcgtccgcg tgaccttcta cggtgacttt gtaagacggg ttgctacgca
     1741 ggaagtttgc atgtgcatcc agcatttgag cgaagtcaga acggatatcg tacttgtcca
     1801 gatcgaagta aacgatgttg ttctgctgca gctgttgcat ttgcagacga gcctgctctt
     1861 cggaagacat gttgccgttg ccgccgttcg catccatacc agtgccggca cccagcatgc
     1921 cttcgctgcc gtcattgctg gcgttcttgt tggaagaaca tgccgcaatt gccataacag
     1981 gcagagcaat catcagccct ttcagcactt tgttcagttg catttcaatg attcctttac
     2041 tattcaatta attattatca cagatacggc gaccaggcag ggaatttgac ctgtccatca
     2101 gttgccggaa gacgcgcttt gaaacgccca tctgtagaaa ccaaattcag cacggatccc
     2161 atcccctgag aagagctgta gattaccata gtgccgttag gtgccagact tggcgtttca
     2221 tccaggaacg tggacgacag aacttgtacg cctcccgttg ccagatcttg tttggcaatg
     2281 tgctgctgcc caccgttgga gctgaccatt accataaatt taccgtcgct gctgacatcc
     2341 gcatcctggt tctgcgaacc ttcccaggta atacgttgtg gcgcaccgcc gttgatattc
     2401 actttataaa cctgtggacg accggcctgg tcagaagtaa atgccaggtt ctggctgtcc
     2461 gggaaccagg tcggttcggt attgttactg cgaccatcag tcacctggcg gatctgacca
     2521 gaagccaaat ccattacgta caggttcagg ctaccggttt tcgacaaggc gaatgccagt
     2581 ttgctgccgt ctggcgagaa tgcaggcgca ccgttgtgac gcgggaatga agccacctga
     2641 cgtacagcgc cgtttgccag cgtttggata actagcgcgg aacgaccgct ttcgaaggtc
     2701 acataagcca gtttagaacc gtctggtgac cacgccggag acatcagcgg ctgtggtgaa
     2761 cggtgaacga caaactggtt gtaaccgtca tagtcagata cgcgcagttc atacgggaac
     2821 tgaccgccgt tggtctgaac aacgtaggca atacgggtac ggaacgcacc tttaatgccg
     2881 gtcagttttt caaacacttc atcactggcg gtatgaccag catagcgcag ccactgcttg
     2941 ttcactttgt acgagttctg agcaagtaca gtacccggtg cgccgccagt gtcaacaagt
     3001 tgataagcaa cattgtaaga accatccgga ttcggagtga cctgaccgac aactacagcg
     3061 tcaataccca gcgcggacca tgcagctggt tgtacttcct gcgcactacc cggctgctgt
     3121 ggcagacgag cgcgatctaa cggattaaat ttaccgctgt tacgcaagtc ggcagcaacg
     3181 atgccgccaa tatcttcagg tgccgcacca gggcccgccc actggaaagg aacaacacca
     3241 ataggacgac cggaatctac accgctgtcg atcacaatgc ggacttcagc atgcagaact
     3301 gatgcccaca gtatgagaaa accaaatgct actcgtaatg cctgcttcat catatctccc
     3361 ttatctggac cgatggccca cgataattta gcagaatgtt aacaaactaa aatacacaaa
     3421 actaccagaa ccccgtggca accggtgcct gatgttgacc gtcggaacag tcaacatcgc
     3481 gattacggtt tgaagtccaa tggcgcgttt ttgaacactt catatactgc ctggcttggt
     3541 ggtttcggga tcttcgcaag tttagctgct gccaacgcag cctgacaaag tgcgggatcg
     3601 ccaccttcag gtttgatatc cagtaacata ccatcgggtg ccagttttat gcgcagcgta
     3661 caggttttgc ctgcatagga cgatgcgtca tagaacttac tttcgatagc agatttaatc
     3721 tgcccggcat agttattgat atcggcccct gatgcgccat tgtttttagt attaccactc
     3781 ccggcagacg aagcattgtt ccctttcgcc cctccccccg ttttcggtgc attcttacca
     3841 gagcttagct caccgaaaat atcatctgcc tctgcggcag cttttgctgc agcggctttt
     3901 tcagctgctg cttttgccgc tgccgctttt ttgtctgcgg cggctttttc tgccgctgct
     3961 tttgccgctg ccgctttttt gtctgcggcg gctttttctg ccgctgcttt cttatcagct
     4021 gctgccttat cagcagccgc tttttctgct gcttttttgt cggctgcagc tttctctgct
     4081 gccgctttct ttcttgcttc agctgcagca gcttctgccg cttccgcttt cttcttcagt
     4141 gccgcagcgg ctgcctcggc ttttttctgc gcttcggctg cggctttggc ggcttctgct
     4201 tctgcttttt tcttcgcgtc tgccgccgct ttcttcgctg cttcttccgc agctttagca
     4261 tccgcttcgg ccttcgcttt agcatctgcc gccgctttcg ctgccgcctc ttccgcttgc
     4321 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ttcctgagca
     4381 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
     4441 agttcttcag cagcctgctg ttcctttatc ttgcgctgct catcagaacg cttcgcgctt
     4501 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
     4561 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
     4621 ctccagagca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
     4681 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
     4741 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
     4801 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
     4861 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
     4921 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
     4981 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
     5041 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
     5101 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gtacaatgtt
     5161 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
     5221 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
     5281 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg aggcggttat aagccattac
     5341 cgccggaatt gcggcgaaca gaccaattgc agtcgcaatc aacgcttctg cgataccggg
     5401 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
     5461 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
     5521 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
     5581 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
     5641 tttgaacccg ctgtaaaata tttgttccga accagtcaga ttatcccgtt tcccctgact
     5701 ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc
     5761 ttcgcgcgcc gctgcgttaa gaatacgggt ccgctggata ataatggccc aggatgcgat
     5821 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
     5881 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
     5941 caaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
     6001 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
     6061 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtacgtaata ttccaccgtc
     6121 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
     6181 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
     6241 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
     6301 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
     6361 cgcgcgattg aggtttggga atgagggcaa gttaagggag cgtaaaaatt tatgggcctg
     6421 gagcggccca taatttttta aagaaaattc tgatttaaaa gaagaagaaa atcagcccga
     6481 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
     6541 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
     6601 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
     6661 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
     6721 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
     6781 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
     6841 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
     6901 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaagc ggtgtagagc
     6961 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
     7021 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
     7081 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
     7141 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcgg aagaaccaca
     7201 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
     7261 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
     7321 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
     7381 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
     7441 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
     7501 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
     7561 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
     7621 agcggcggaa cgaaactacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
     7681 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
     7741 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
     7801 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
     7861 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
     7921 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
     7981 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
     8041 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
     8101 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
     8161 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga gttcgccaca
     8221 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
     8281 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
     8341 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccaga
     8401 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
     8461 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
     8521 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
     8581 accgcctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
     8641 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
     8701 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
     8761 tcctcttcct gatcaggaat gccgaacaga gtaaaggcag caggcgcagg ttgcgtttcc
     8821 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
     8881 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
     8941 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
     9001 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
     9061 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
     9121 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
     9181 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
     9241 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
     9301 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
     9361 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
     9421 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
     9481 tagaccgttt ccataatggc cagcaggaac gccataccga gcgtcagtgg cacaaaaagg
     9541 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
     9601 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
     9661 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
     9721 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
     9781 ttacgctgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
     9841 cggtgatcaa tttatagcta aattaccgcc tttcagccaa tttgatcgag aacaatttat
     9901 ctctttttga tgccaatttc caagacttat acattgataa atatcaattc ctacaaatta
     9961 tgttaatcat gtttcaataa ggtaacttta gtgacattta tgtttaaaat gtgtgagtta
    10021 taaggctgaa attagtttcg gttggcaatt taaagataaa aagagacgaa gcaaaaacag
    10081 cacggttgac cttaattctt tttttggtca ctggctaata cgacgcagag tttcaggatt
    10141 actaataaag gcataaatcc accccgccga tattaacatt taattaactt gtaattatat
    10201 tataattaac tcacccaggg ttattatttt gttaaaaata aagccacata tacgtggctt
    10261 gagagtgaag ataaacgcaa aattacctga tgcgctacgc ttgccagtgt ctacatcatc
    10321 tctgcaatat attgaatttg cctgcttttg tagaccgaat aaagcgttca tgccgtatcc
    10381 ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc atatgtggct tcggtttgat
    10441 tgctattcag gcaagccggt aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga
    10501 tagatttgaa ccttgatttt cctcggtggt aatgtgttca tccatcatcg tttctgagca
    10561 agaaatcacc tcgcgactga aagcaacagt cgcatcacag gttgctgatt cagccggatt
    10621 aaacagccgt aaaatcactt cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca
    10681 tcccactggg ggcattttca acaaactata actttccggc acgttgaatc cggctttgtt
    10741 gagcttcatc gcatcccatg ggattttgtt gtaacactgt actggagtca gccatgctcg
    10801 cgcctgctga gctacaccag cggcggttgg cgtaccggta taactcaata aacttaggcg
    10861 acaagaaagc agaccacgta gttgtgagtc cgggactggc attttaattc ccgaaggccg
    10921 cccaggcctt aaaagcagat cttctttgcc cagtaagccc acgccacgca gcaacgtaat
    10981 ggcaaaggtt ttcttctctt caccgatgac ttcaaattca cgtaaccctt cgctaaagac
    11041 agccatgccg ttacgccctt cctgtaaggc aacatagttg agcatattcc ataccggaac
    11101 cggcgcttct ttccagcctt cttgctgcca gttgttcatt gcactgtcgt tcacggggcg
    11161 cgttagcgaa ccaaactgcg tatctgccag aacactgtca gtgttaaaag gtgtagggac
    11221 caggacacga aggcgatgat cgtcagcctg gttatcaagg ttgatatcca catcaatacg
    11281 cctgctgtta tgactaagag tgacaaccaa caccccccct actctgccag gcttcatgaa
    11341 taatatcgca ttgcggtttc gcgttcgctg cggtaattac ccactcttct tttgcgggtg
    11401 aatagtcata ttcatcacca tcatcagagc tttcttcaat ttgcaatacc cggtcatagc
    11461 gcacaccgct gtctttatct accagttgca gagaaccatc ctcattgagc gcaatctgcc
    11521 agaaagcatt ttccagaatc ccttcagcgt cactttttgc cgcaatcacg ttgccaggct
    11581 gattcgcttc aatataaagc gtgcgatagc ccatagaagg gacaatctgg ttgatctgaa
    11641 tatcaaactc cataaaggga tcataattgc cataatgaac aatttgccga tcgattaggc
    11701 caggatcgat ctcacgggca tggcgaataa aatacggtac aggctgaccg cgatcgtctt
    11761 gtagattaaa ctggctggcg cgcagccgca cagtggtgtt aataacttct tcccgcggcc
    11821 agggcatcag gttaaacagc acgagtttgt cggcatcact ctgtggcatg ttatcggcaa
    11881 ttttgcgcat gtagaaacgc agcagattat ccgccatgtc ttcagccagt tcgaagcggg
    11941 cgacaatttc gcgatgaact ttgtcactgc aacaacaacc gatactgtcg tgggcatgat
    12001 tttttaagat ctctttccac attttctcca gcaagccgtg gtggtattca aaacccaacg
    12061 tccaggccag cgttgccagc ggttccagca gattaacaat cttattttca atacgtgcgt
    12121 gggcaatttt gatatccatg cgcgtggaac cgatggtgcg atgtacgcgc atatatttgc
    12181 catcgataaa ttcacctttc agggtggcca gactttcacg ctgcgcttcg atcttctcaa
    12241 atacctcttc aaagcggctc atcacaaact tacgttgagg gtagatctca cgtagcttat
    12301 ccatcacttc gaagatattt tgctgcaatg gcatctggtc atgaccattc ggcagcaaaa
    12361 tctctttggt tacagacgct ttttccagca cgtcaaaata actgtcgagg cgtttacgta
    12421 atccgttttc gtcggcaggt aagtacttac cgatggcgta gcccagcggc agcacctgcg
    12481 ccgtcacttc gctaccgtca ctgctttgcc acaaaaactc ggttttatca gtaccgtggc
    12541 gctccgaaca tccgcgccag aacatagtgc gggtaatgcc aaatccattg taaatatgcg
    12601 gcagttgccc ggacatgcca aaggaatccg gtaaataacc tattttcatc ggctcgccaa
    12661 acgcgaggca gtcacgcatt ccgtacatca gattacggac gatggattcc gcagaaacaa
    12721 tcgtggtatc ggtctgggta taccaggggc cgataatcaa cttaccggct tctacctgtt
    12781 tcttcacgcg gtctttgttt tccggtttca ccgcgaaata atcttcgagg atcgccgttt
    12841 gcccgtcgag tacgtaatat ttgtattcgt tgtcctgttc cagtcggcac aggatctctt
    12901 ccatattatt gaccagcaga atgcgtgact cttcggtggt gaaataccac tcccgatccc
    12961 aatgcatatg cggggtgatg tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt
    13021 ttatggcatt acgccatcag tcagatagtt gccatgctta accgcgtgac gccgccagat
    13081 caggagaatt gcagtcgaga ttgcagcccc caccaatgcc gcaccaaacc agccaattgc
    13141 cgccataaca ccgcccgcgc cattatcatg aagtaaaaag agcgagaaaa tgccggcacc
    13201 gggtgtcgaa aggccgatat tcatcgcacc gacaatagcg cccgttacca tagagcccag
    13261 cacaaacgaa ccaataaccc gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt
    13321 aatacctgcc agccctaaca gccaggtgga tttcccggtc tcaatttcaa actctttaaa
    13381 caggcgcggt gcgagcatcg tggaagcggt tacggtaaat gccgaaacca ttttgacgga
    13441 ggcgaaaatg gcatacgggc cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata
    13501 agcggctttg ttcactggcc ctccaaggtc aaaggaacac ataaaaccga gaatggcacc
    13561 cagcaacagc gcgttacttc ctgacagacc gttcagccag gcggtaagcg agttattgat
    13621 ccatgcgaca ggttctccca ccacaaacag catcagactg cccgctccca acgtaccgag
    13681 caccgggtag agataaaaag tcaggaatcc attgaattta ctgctaagac gcaagtgatt
    13741 tttcacccag cgcatcaagt aaccggctat caatccgcca acgaccgcgc cgagaaaccc
    13801 ggagccgatc atgttggcgg caagtccagc cgcaaagcct ggcgctaacg ccggtttatc
    13861 tgccagagaa taggtggtat aggccgcgag caccggtacc atcaaaattc cgagcagccc
    13921 gccgcccagc ttgcggtaca tccacagcca ggagttttct tcattaaaca gatcttgtag
    13981 cccgaagatt tgcgacagta ataccgcgac cgccagcacc gtgccccccg cgacaatcaa
    14041 cggtacggca aaagagattc cgctcaacag tgcctgtttc agctccgttt tgacactttt
    14101 caccggttgc gtatcttgct gtacggtacg cgtctcatcg ctacgcttga gggtaagcgc
    14161 ttgttggatc aacgcttctg catggcgaat cggctcggca acaggcactg aaagcgcggg
    14221 aatgccatta aaacgctcac tctccttgat ggcgacttca gccgcaaaaa tacaggcggt
    14281 tgcactattg agttgatccg ccgttaaacg cccttcaatg ccgttagcgc cttgtttttc
    14341 aacgtaaaca tttacgccga gtttgcgtcc ggctttttcc agatattccg cagccatata
    14401 ggtgtgagca atacccgccg gacaggccgt tacgcagacg atagttggcg cgttggaaaa
    14461 agaggcagaa ggttgcgtgc ctcccttgtc atccagcgcc gagagcaact catcaggcgt
    14521 cgtcgccgac tgtatacgcg cccgaatctc atcatccgca aggcgcgtgg tcagcgctgt
    14581 cagcagttgc atatgcgtag tacccgcttc attgggggga atcgccagca gcaccactaa
    14641 atcaactgct tccgggccat caacgccttc ccactgaagc ggctcgctga gtgtggcgac
    14701 cgcaaacgcc gcttctttta ccgcagcagt tttgccatgc ggcacagcca acccttcacc
    14761 taaggcggtc gggccaaggc tttcacggcg atacacttct tccagaaatt gctcagtact
    14821 ggaaattttc cccagagcag caagacgttg agtcaacgcg tggatggcct cttcacggct
    14881 ggtaaagcgc gcattcagac acaacgcatc gcggtgggtc agagtcgtca gattcatcat
    14941 gatcctcact tgatcgtttt tatagtcgcg ccagtgtgcc cgataaaaca ataccatcat
    15001 gtgatcaatt tcacttgatt gaaattattt gtattgtatt tgtattattt acaggatgaa
    15061 tttatcgggc ataatggcta aattcatatt aatgagatac taaaaatggg acacaagccc
    15121 ttataccggc agattgccga tcgcattcgt gaacaaattg cgcgtggcga gcttaaacct
    15181 ggcgatgcgt tacccactga atcagcgcta caaacagagt ttggcgtcag ccgggttacg
    15241 gtgcgtcagg ctttgcgcca gctagtcgag cagcagatcc tcgaaagcat tcagggcagc
    15301 gggacttacg tcaaagaaga gcgggtcaat tacgatattt ttcagttaac cagttttgat
    15361 gaaaaactgt cggaccgtca cgtcgataca cacagtgaaa tcctgatatt tgaagtgatt
    15421 ccggctgacg attttcttca gcaacagcta caaatcaccg cgcaggatcg tgtgtggcat
    15481 gtgaagcgcg tgcgctatcg caagcaaaag ccaatggcgc tggaagaaac ctggatgccg
    15541 cttgcgttgt tcccggatct cacctggcag gtcatggaaa attcgaaata tcactttatt
    15601 gaagaagtga agaagatggt tatcgatcgt agcgaacagg aaatcattcc tctgatgcca
    15661 accgaagaga tgagccgtct gcttaatatc agccagacaa agccgattct tgaaaaagta
    15721 tcgcgtggat atttagttga tggtcgggtg tttgaatata gccgcaacgc ttttaatacc
    15781 gatgactata aatttaccct gatagcgcag cgaaaatcat cgcgataagc acaaaaaagg
    15841 ccatcatacg atggccaacc atgtcgaaac cgacagccct tcgccattcc tgtagaggga
    15901 agcggcgagg gctatttctt attacagata tttatttcag aacagttttc agtgcttcac
    15961 cgatatccgc caggctgcga acggttttca cgcctgcggc ttccagagca gcgaatttct
    16021 catccgcagt ccctttccca ccagcaatga tcgcacccgc gtggcccata cgtttgcctt
    16081 tcggcgcagt cacaccagcg atgtaaccta caactggctt ggtaacgtgc tctttgatgt
    16141 acgctgccgc ttcttcttca gcgctaccgc cgatctcacc gatcatcacg atcgcttcgg
    16201 tctgcggatc tttttcgaac atttcgagga tgtcgataaa gttagagcct gggatcgggt
    16261 caccgccgat accaacacag gtcgactgac cgaaaccgta atccgtggtc tgtttaaccg
    16321 cttcataggt cagtgtaccg gaacgggaaa cgatacccac tttacccggt ttgtgaatgt
    16381 gacccggctg gataccgatt ttgcattcac ccggagtgat aacgcccggg cagttcgggc
    16441 cgatcatacg aacgccggct tcgtccagtt tcactttcac agtcagcata tccagcgtcg
    16501 ggatgccttc agtgatggtg ataatcagtt tgatgcctgc atcaatagct tccagaatgg
    16561 agtctttgca gaacggtgct ggtacgtaga taacagaagc ggtagcgcca gtagcagcaa
    16621 cagcttcacg cacggtgttg aacaccggca ggccgaggtg ggtagtaccg cctttacctg
    16681 gggttacgcc gccaaccatt ttagtgccgt atgcaatggc ctgttctgag tggaaagtcc
    16741 cctggctacc agtaaagccc tggcagataa ccttggtgtt tttatcaatt aaaatggaca
    16801 ttatttcccc tccactgcgg caacaacctg ctgagctgca tccgtcagac cttttgctgc
    16861 aataatattc aggccgctgt cagccagttt cttcgcgccg agttcggcgt tgttaccttc
    16921 cagacgtacc acgaccggta cgttaacacc cacttctgct accgcgccga tgataccgtc
    16981 agcgatcagg tcgcaacgaa cgataccgcc gaagatgtta accagaacgg ctttcacttt
    17041 gtcgtcagag aggatgattt tgaacgcttc ggttacacgt tctttggttg cgccgccgcc
    17101 aacgtcaagg aagttagccg gttcgccgcc gtgcagttta acgatgtcca tcgtacccat
    17161 cgccagacct gcgccgttaa ccatacaacc gatgttaccg tccagcgcaa cgtagttcag
    17221 ttcccactgt gcagcctgtg cttcacgcgg atcttcctgc gactggtcac gcatttcgcg
    17281 cagatcaggc tggcggaaca gtgcgttgcc gtcagcgccc agtttgccgt cgaggcaaat
    17341 cagatcgccc tgtttggtga tgaccagcgg gttgatttcg atcaacgcca ggtcgcgctc
    17401 caggaaaatg gtcgccaggc ccatgaagat tttggtgaac tgctgaacca gtttaccttc
    17461 cagacccagt ttgaacgcca gctcgcgtcc ctgatacggc atcgggccag tcagcggatc
    17521 aagcgcaact ttgtggatca ggtgcggagt ttcttccgcc actttttcga tttccacgcc
    17581 gccttcggtg gaggccataa agaccacacg acgggaacta cggtctacaa cggcaccgag
    17641 atacagctct ttagcgatat cggtcgctgc ttcaaccaga atctggttaa ccggttggcc
    17701 attggcatct gtttgatacg ttaccagacg cttgccgagc cagttttctg caaaagcacg
    17761 gatgtcttct ttgctgttta caactttcac accgcccgct ttaccgcggc caccagcgtg
    17821 aacctgacat ttcactaccc acgggccggc accgattttt gaagcggctt cttctgcttc
    17881 gcgcggagta gtacaggcat aacccaccgg tgctggtaag ccatagcggg caaaaagttg
    17941 ttttgcctga tattcatgta agttcatgtg ttctgtccat ccttcagtaa tcgttatctt
    18001 ttaaaccgta gaccgacacc ctatccacgt cggtctacag tgcaggtgaa gcttaaacta
    18061 ctacacgtcc agcagcagac gcgtcggatc ttccagcaac tctttgatcg ttaccaggaa
    18121 gcccacggat tcgcgaccat cgatcagacg gtgatcgtag gacagcgcca ggtacatcat
    18181 cggcaggatc tcaacctgac cattcaccgc catcggacga tctttgatag cgtgcatacc
    18241 cagaattgcg ctctgcggcg ggttgatgat cggcgtagac atcagggaac cgaacacacc
    18301 accgttggtg atggtgaagt taccaccggt cagatcttca accgtcagct tgccgtcacg
    18361 gcctttaact gccagctctt tgattttctt ctcgatgtct gccatgccga gggtatcgac
    18421 atcacgcagt accggcgtca ccaggccgcg cggcgtggaa accgccatgc tgacgtcgaa
    18481 atagttgtgg taaaccacgt catcaccgtc gatagaagca ttcacttccg ggtaacgttt
    18541 cagggcttca accaccgctt tcacgtagaa ggacataaag cccagacgga tgccgtggcg
    18601 tttttcaaac gcttcaccgt actgcttacg cagatccata atcggcttca tgttgacttc
    18661 gttgaacgtg gtcagcatgg cggtggagtt tttcgcttcc agcagacgct ctgccacacg
    18721 cttacgcagg cgagtcatcg ggacacgttt ttcactacgt gcagccagag ccggttgcgc
    18781 cgccggagca gccgctgccg gagcagactc tttcgccggg gctttcgcca gatgtttttc
    18841 cacatcttca cgagtcagac gaccacccac accggtgcct ttaatggcgc tggcgtcgag
    18901 attgtgttca gccagcaggc gacggatcgc cgggcttaac gcatcgttgt tttgctcttc
    18961 cagagacgcc tgctggcgtt gcgccggagt ggacgctttc tcttcagatt tggcgctggt
    19021 ttctttaccg gcgctgttgc cttcacgcag gcgaccaagg atctgacgag acgttaccgt
    19081 tgtaccttca tcttccagaa ccgcatccag aatgccgtct gctgatgccg gtacttccag
    19141 taccactttg tcagtttcga tttctaccag cacttcatca cgtacgactg cgtcgccggg
    19201 ttttttatgc caggttgcga cggtggcatc ggctacggat tcaggcaggt cagggaccag
    19261 aatatctacg ctactcattg tgtatccttt atttattcga cgttcagcgc gtcattaacc
    19321 agatcttgtt gctgtttctg gtgaacggac atatacccta ccgccggaga ggcggaggcc
    19381 gtgcggcctg cataacgcag agaagcccca aacggaatca cttcacggaa atgatgctgg
    19441 ctgcagtacc atgcgccctg gttgagcggc tcttcctggc accagacaaa atccttgacg
    19501 tgagcaaact gctgcaacac ttcctgcatc gctttatgcg ggaacgggta gagttgctcg
    19561 atacgcacaa tggcgacatc gtgttgattg ttcttacgac gctgttccag caggtcgtaa
    19621 taaaccttac cagaacacat cactacgcgc ttcacgccct tcggatcaag ctcgtcgatt
    19681 tcaccgatgg ctggcaggaa ggtgccgttc gccagttctt cgaggctgga aaccgccagc
    19741 ggatgacgca gcagggattt cggcgacatc actaccagcg gacgacgcat cccgcgcagc
    19801 gcctgacgac gcagcatgtg gtaaacctgt gccggggtag acggaacgca aacctgcatg
    19861 ttttgctcag cacaaagttg cagataacgt tccagacgcg cggaggagtg ctccggcccc
    19921 tgcccttcgt aaccgtgcgg cagcaacatc accagaccac acatccggcc ccatttctgt
    19981 tcgccagaag agatgaactg gtcgataacc acctgcgcac cgttggcgaa gtcaccgaac
    20041 tgcgcttccc agatggtcag agtgcgtggt tctgcggtgg cataaccgta ttcaaacgcc
    20101 agcactgctt cttcagacag tacggagtcc cagacgcgga acgcgccctg cccgttatgg
    20161 atgtgttgca acggcgtgta agtggaaccg ttagactggt tgtggatcac cgcgtggcgg
    20221 tggaagaagg taccgcgacc ggagtcttca cccgacaggc gaaccggaat gccttcgtca
    20281 accagcgtgg cgtaagcgag gttttccgca ccgccccagt cgaacagttt ctcaccggca
    20341 gccatcgcct ggcgatcgcc ataaatcttg gcaacgcgag actgcatttc aactgcttcc
    20401 ggcaccgtgc tgatgcgttt cgccagctcc tgcaggcgct tcatctcaac tttgttcggg
    20461 tactcttcgt cccattcgtg gttgaggtac ggcgaccagg tgaaagagtg catgttcatc
    20521 ggacgccact ctgccactac gcaatcgcca gcatccagcg catcgcggta caggttaacc
    20581 atctcggtgg catcttccag cgtcgccact ttttcctgct ccagcttgtc agcgtagatt
    20641 ttgcgcggtg tcggatgttt tttgattttc tgatacatca gcggctgggt tgcgctcggc
    20701 tcgtcggctt cgttgtggcc gtgacggcgg tagcacacca ggtcgatgaa gacatcacgt
    20761 ttaaaggtgt tacggaaatc gagcgccaga cgggtcacaa aggcaacggc ttccggatca
    20821 tccgcgttaa cgtggaaaat cggtgcctga accatcttac cgatatcagt acagtacggt
    20881 gtagaacgag catccagtgg attagaggtg gtgaaaccaa cctggttgtt gataacgata
    20941 cgtaccgtac cgccaacttc ataaccacgc gctttcgaca tgttcagagt ttcctgaacc
    21001 acgccctgcc cggtcactgc ggcgtcaccg tggatggtga ttggcagcac tttgttgctg
    21061 ctcggctcat caagtctgtc cagacgggca cgaacagaac cgataactac cgggcttaca
    21121 atctcaaggt gagacgggtt aaacgccagc gccaggtgca ccaggccgcc atcggtctgg
    21181 aagtcagacg agaagcccat gtggtatttc acgtcacccg tgccgaggtg ttctttatgt
    21241 ttaccggcaa actcgtcgaa caagtcttgc ggttttttac ccagcacgtt caccagcacg
    21301 ttcagacgac cacggtgcgc catcccaaga accacttcgc gggtgccgct gttgccagcg
    21361 tggcggatca tctctttaag catcgggatt aacgcgtcac cgccttccag cgagaagcgt
    21421 tttgcgccag ggaattttgc gccgaggtaa cgttcaaggc cttcagcggc ggtcagttcg
    21481 cttaagaagc gttttttctc ttcgctattg aaagtcgcgc gaccagactc gatacgctgt
    21541 tggatccagc gtttttcttc ggtgctggta atgtgcatat actcggcacc aatcgggcca
    21601 cagtaggttt gcttgagggc ttccagcagc tcgccgagtt tcatgttttc tttgccgctg
    21661 gcaaatgaac cgacgttgaa ggtctcctgg aagtctgctt cggtcagatc gtggaaagac
    21721 ggatccagat cggccacttt atcttgctgc cacagtccca gcggatcgag attcgcatgc
    21781 tggtgaccac ggaagcggta tgcgttaatg agctgcagga ctttaacctg cttcacattg
    21841 gtgtcagggt cggagatcgt tgaagagtaa cgtgaagcgt ctttcgccag gcggcggaaa
    21901 tattcacgcg tttgagagtg gaattgatcc ggtttgactc ccgtaccagg taactgctgg
    21961 aacgtcgaac gccagttagc gtcaaccgag tcaggatcgg ttaagaagtc ttcatagagc
    22021 tgttctatcc agctctggtt tgcgccagag aggtaagaag agtccaacca ggctttcaaa
    22081 gcgctgttct gcatcgtgat cccttaagca tcttttttat gcttacttcg ccgtggatac
    22141 taccacgcac agtgatgtgc gtgccggggt tcacttgcga gctcttgtct atgtagtggc
    22201 tcgcgaagga acctttaaaa actgtcttag tgtcaggggc agtttttaaa gatttcctgc
    22261 atttctttgt tacgcctgat gcgctgcgct tatcaggcct acgggtaatg acttgtaggc
    22321 cggataaggc gttcacgccg catccggcac tggttgcctg atgcgacgct tgcgcgtctt
    22381 atcaggccta cggtttacgc attacgttgc aacaacatcg acttaatatg gccgatggcg
    22441 cgcgtcgggt tcagcccctt cggacataca ctgacgcagt tcatgatgct gtgacagcgg
    22501 aatacgctga atgcatcgct caaaccgtcg aggcggctgt cagtctcggt atcacggcta
    22561 tcgatcagga aacgatatgc cgctaacaag cctgccgggc cgataaactt atcgggattc
    22621 caccagaaag acggacaaga ggttgaacaa catgcgcaga gaatacattc atacagcccg
    22681 tcgagttttt cgcgctgctc tggcatctgt aaatgctcgc gagctggcgg attttgtcca
    22741 ttattcaaca ggtaaggctt aattttctca tattgcgcat agaattgtcc catgtctacc
    22801 accaaatcgc ggatcaccgg taaacctggc agcgggcgaa tcacaatctt cttgcccggc
    22861 tggttgagtg ccgaaatcgg ggtaatacag gccagaccat tcttgccgtt catgttcaga
    22921 ccgtcggaac cgcacacacc ttcacggcag gagcggcgga acgacaggct gggatctttc
    22981 tctttcagct ggataagcgc atccagcagc atcatgtcgc gaccttcttc cgcttccagg
    23041 gggtaatcct gcatacgcgg agcatcatca acatccgggt tatagcgata aattgaaaac
    23101 tcgagtctca ttttcctgtc tccgcattag taagtacgaa tcttcggcgg gaatgccggg
    23161 cgcagtttcg gttccatgtt gacgcttcgg cgcgtcatgg attccgactc tggcagatac
    23221 agggagtggc acagccagtt ttcatcatca cgatccggga agtcgaagcg gctatgtgcg
    23281 ccacggcttt cggtacggaa gttggcagaa acagccgttg catacgccgt ttccatcagg
    23341 ttatccagtt ccaagcactc aacgcgctgg gtgttgaact cgctggaagt gtcatccaga
    23401 cgggcatttt tcagacgctc gcggatcact ttcaactgct caagcccttt cgccatcgca
    23461 tcaccttcac ggaagaccga gaagttatgc tgcatacatt cttgcagcgc tttacgaatc
    23521 gccaccggat cttcaccgtt acgattattg ttccagcggt gcagacgatc cagagatgct
    23581 tcaacatcag actcgctggc atcgcgcaat gcgccctgtt cggcgataga ctcttgcaga
    23641 tgcagacctg ccgcacgacc aaagaccacc aggtccagca gcgagttgcc gcccagacgg
    23701 ttagcgccgt gtaccgatac acaagcgatt tcaccaacgg caaacagccc cggaacaacc
    23761 acatcttcgc ctttctcgtt caccgtcagc gcctgaccgg tcactttggt cggaataccg
    23821 cccatcatgt agtgacaggt tgggataacc ggaatcggct ctttcaccgg atcgacgtga
    23881 gcaaaggtac gggaaagctc caggataccc ggcagacggg attcgagaac ttctttaccc
    23941 aggtgatcga gtttcagttt cgcgtgtggc ccccacggac catcacagcc gcgaccttca
    24001 cggatttcaa tcatgatgga acgcgccacc acatcacggc ccgccaggtc tttggcgttc
    24061 ggcgcataac gctccataaa gcgttcgcca tgtttgttca gcagataacc gccttcacca
    24121 cggcaacctt cggtgaccag tacgcccgca ccggcaatgc cggtcgggtg gaattgccac
    24181 atttccatat cctgcaccgg tacgcctgcg cggatagcca tgccgacacc gtcgccagtg
    24241 ttaatgtggg cgttagtggt ggactgataa atacgccctg ccccgccagt cgccagcacg
    24301 gtagcgcggg ctttgaaata aaccacttca ccggtttcga tgcacagtgc ggtacaaccc
    24361 accaccgcgc catcctggtt tttcaccaga tccagcgcat accactcgga gaaaatggtg
    24421 gtgtggtttt tcaggttctg ctgataaagc gtgtgcaaca gtgcgtgacc ggtacggtca
    24481 gccgccgccg cagtgcgtgc cgcctgctcg ccgccgaagt ttttcgactg accgccaaac
    24541 ggacgttgat agatacgacc atcatcaaga cgcgagaacg gcaggcccat gtgttcgagt
    24601 tccagaatcg cttccggccc ggttttacac atatattcaa tcgcgtcctg gtcaccgata
    24661 tagtccgacc cttttacggt gtcgtacata tgccattccc agttatcttc atgggtatta
    24721 cccagcgcaa cggtaatgcc gccttgcgca gaaacggtat gggaacgggt cgggaagact
    24781 ttagagagca gcgcacaggt ctggccgctc tgggaaattt gcagcgccgc gcgcatacct
    24841 gcgccaccgg caccaatcac aactgcatca aattctctga ctggcaattt catcacacac
    24901 cccacaccac aacgaatcca taaatcacgt aaaccaccag tgcaacgaca atcaccagtt
    24961 gcagcatcag gcgcaatgcc agcggtttaa cgtagtcggt caacacctgc cacatgccga
    25021 tccaggcatg aatcaagata gaaaacagcg ccagcagggt gaacactttg gtgaacgcag
    25081 aggcgaagaa accgatccag acttcatatg tcagttcgcc actggtggcg aaaaaaccga
    25141 ccatataaat gatgtagagc gtcaggacga tagcggtagc gcgaacgagg atgaaatcat
    25201 gtacgccatt gcgtcctaat gcggaggcgt tgcttaccat acgaggactc ctgcgagaag
    25261 tgaaagcacg acagtaataa caaaggagat tttggcggag cgtttacccg cttcgaatgt
    25321 ttcttccaga tagccaaaat ccatcatcat gtggcgaata cctacgacga cgtgatacgc
    25381 cagagcggta aggatgcccc acatgataaa tttgacgaag aagctgccca taatcgcgga
    25441 agcttgctcg aaaccttcag gggaagagag gctggtaccc agaagccaca gcaggatgcc
    25501 cactgcaaca aaggtgatca caccggaaac gcgatggaga atggacgcta tcgccgtgat
    25561 ggggaaccgg atggtctgta ggtccagatt aacaggtctt tgttttttca catttcttat
    25621 catgaataac gcccacatgc tgttcttatt attccctggg gactacgggc acagaggtta
    25681 actttctgtt acctggagac gtcgggattt ccttcctccg gtctgcttgc gggtcagaca
    25741 gcgtcctttc tataactgcg cgtcatgcaa aacactgctt ccagatgcga aaacgacacg
    25801 ttacaacgct gggtggctcg ggattgcagg gtgttccgga gacctggcgg cagtataggc
    25861 tgttcacaaa atcattacaa ttaacctaca tatagtttgt cgggttttat cctgaacagt
    25921 gatccaggtc acgataacaa catttattta atttttaatc atctaatttg acaatcattc
    25981 aacaaagttg ttacaaacat tgccaggaaa agcatataat gcgtaaaagt tatgaagtcg
    26041 gtatttcacc taagattaac ttatgtaaca gtgtggaagt attgaccaat tcattcggga
    26101 cagttattag tggtagacaa gtttaataat tcggattgct aagtacttga ttcgccattt
    26161 attcgtcatc aatggatcct ttacctgcaa gcgcccagag ctctgtaccc aggttttccc
    26221 ctctttcaca gagcggcgag ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg
    26281 caaatttaag ttccggcagt cttacgcaat aaggcgctaa ggagacctta aatggctgat
    26341 acaaaagcaa aactcaccct caacggggac acagctgttg aactggatgt gctgaaaggc
    26401 acgctgggtc aagatgttat tgatatccgt actctcggtt caaaaggtgt gttcaccttt
    26461 gacccaggct tcacttcaac cgcatcctgc gaatctaaaa ttacttttat tgatggtgat
    26521 gaaggtattt tgctgcaccg cggtttcccg atcgatcagc tggcgaccga ttctaactac
    26581 ctggaagttt gttacatcct gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa
    26641 tttaaaacta cggtgacccg tcataccatg atccacgagc agattacccg tctgttccat
    26701 gctttccgtc gcgactcgca tccaatggca gtcatgtgtg gtattaccgg cgcgctggcg
    26761 gcgttctatc acgactcgct ggatgttaac aatcctcgtc accgtgaaat tgccgcgttc
    26821 cgcctgctgt cgaaaatgcc gaccatggcc gcgatgtgtt acaagtattc cattggtcag
    26881 ccatttgttt atccgcgcaa cgatctctcc tatgccggta acttcctgaa tatgatgttc
    26941 tccacgccgt gcgaaccgta tgaagttaat ccgattctgg aacgtgctat ggaccgtatt
    27001 ctgatcctgc acgctgacca tgaacagaac gcctctacct ccaccgtgcg taccgctggc
    27061 tcttcgggtg cgaacccgtt tgcctgtatc gcagcaggta ttgcttcact gtggggacct
    27121 gcgcacggtg gtgctaacga agcggcgctg aaaatgctgg aagaaattag ctccgttaaa
    27181 cacattccgg aatttgttcg tcgtgcgaaa gataaaaatg attctttccg cctgatgggc
    27241 ttcggtcacc gcgtgtacaa aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc
    27301 catgaagttc tgaaagagct gggcaccaaa gatgacctgc tggaagtggc tatggagctg
    27361 gaaaacatcg cgctgaacga cccgtacttt atcgagaaga aactgtaccc gaacgtcgat
    27421 ttctactctg gtatcatcct gaaagcgatg ggtattccgt cttccatgtt caccgtcatt
    27481 ttcgcaatgg cacgtaccgt tggctggatc gcccactgga gcgaaatgca cagtgacggt
    27541 atgaagattg cccgtccgcg tcagctgtat acaggatatg aaaaacgcga ctttaaaagc
    27601 gatatcaagc gttaatggtt gattgctaag ttgtaaatat tttaacccgc cgttcaaatg
    27661 gcgggttaat ttttatatgc ccaaacacaa aaaattgtaa aaataaaatc cactatcgga
    27721 cattcatata ttctaaaaaa aatatatcgg tataaattat aaacaaatcg atgattttta
    27781 atcatctatc ttcctctcag ttacatgaat aaaccaccaa atatagcgca cccctataat
    27841 ttccaatact ttattcatca aacatttaat tcataccatc aattaattac ttttacattt
    27901 aataaaatta ataatatttt taagaaaaac taaaaacatt cgacaatcaa ataagatatt
    27961 cttgcaccga tatacttttt tcttttctgt taaaggactc agttatgttt aaaggacaaa
    28021 aaacattggc cgcactggcc gtatctctgc tgttcacagc acctgtttat gcggctgatg
    28081 aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt gaaatacatc
    28141 aggatgatat tgataaagag gttgaactcg gtcaggtgac caccagccac attaatcagt
    28201 cacatcacag cgatgccgtt gctgtcgacc tgcgcttagt caactgtgat ctggaaaact
    28261 ccagcaacgg ttccggtggc aagatttcta aagttgcagt cacctttgat agctcagcga
    28321 aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcc accggcgtcg
    28381 gcgtacgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct actccagata
    28441 tcgacctggc tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg atggaacaaa
    28501 ttgatcaggc tacacctgta acgccaggcg cagttacggc gaatgcaacc tacgtgctgg
    28561 attataaata agtattactt tggcggcgct atcgccgcct ttcataaaat atttatcagg
    28621 tatggacacc gtgaatattt atcgactctc ttttgtatcc tgcctggtcg tggcgatgcc
    28681 ttgcgcattg gcggtcgaat tcaaccttaa tgttctcgat aaatcgatgc gcgaccgcat
    28741 tgatatttca ttattaaagg aaaaaggagt cattgctccc ggtgagtatt ttgttagcgt
    28801 tgcggtaaat aataaccaaa tcagtaacgg gcaaaagatt aactggcaca aaaatgacga
    28861 taaaaccatt ccgtgcatca atgatttact ggtcgataaa tttggcttaa aacctgaagt
    28921 ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt agctcccgac ctgaaatgct
    28981 cttcaatttc gatcaagcca atcagcaact gaatattagt attccgcaag cctggctggc
    29041 gtggcactca gaaaactgga ccccaccctc cacatggaaa gaaggtgtcg ccggtatcct
    29101 gatggattac aacttgtttg ccagcagcta ccgcccacag gacggcagca gcagcactaa
    29161 cctgaacgcc tacggtacca ccggaattaa cgccggggca tggcgcttac gtagtgatta
    29221 tcagttgaat cagactgata gcgatgataa ccatgaacag tcaggcggaa tatcgcgcac
    29281 ctatcttttt cgtccattac cgcaattagg ctctaaatta accctcggcg aaacggattt
    29341 tagttccaat attttcgacg gtttttctta taccggcgcg gcactggcaa gtgacgagcg
    29401 aatgttgcca tgggaactac gcggctacgc cccacaaatt agcggtattg cacagaccaa
    29461 tgccacggtg acgatcagtc aatcaggccg cgtcatttac cagaaaaaag tcccaccagg
    29521 cccatttatc attgacgacc ttaatcagtc tgttcagggc acactggatg tcaaagtgac
    29581 ggaagaagat ggtcgggtga acaatttcca ggtttcggca gcatcgacgc ccttcctgac
    29641 tcgtcaggga caggttcgct ataaactggc cgcgggtcag ccacggccct ccatgtcaca
    29701 tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc tgtcaaacac
    29761 ctcgctgtac ggcggcctgc tgctttctgg tgatgactac cattctgccg caatgggtat
    29821 tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg ccagtagcca
    29881 ttttgatact cagcaggacg agcggggctt aagctaccgt tttaattaca gcaaacaagt
    29941 ggatgccact aacagcacga tttccctcgc cgcttatcgt ttctccgatc gtcattttca
    30001 cagctacgcc aactatctgg atcacaaata caacgacagc gatgcgcagg acgaaaaaca
    30061 gacgatcagc ttattgtggg ccaaccgatt accccactaa acctcaatct ttacgccaac
    30121 ctgctacatc aaacctggtg gaatgcagac gcctccacga ccgccaacat cacggcaggt
    30181 tttaatgttg atattggtga ctggagagat atctctattt cgacgtcatt caatacaacc
    30241 cattacgaag ataaagatca cgacaaccag atttacctgt cgatttcgct ccccttcggt
    30301 aacggtggtc gggttggcta tgacatgcaa aacagtagcc acagcaccac acaccgcatg
    30361 tcgtggaacg atacgctgga tgaacgtaat agctggggca tgtctgccgg actgcaatcc
    30421 gaccgtcctg acaatggagc ccaggtgagc ggtaactatc agcacctgag ttcagcgggt
    30481 gagtgggata tttctggtac ctatgccgcc aatgattaca gttccgtcag cagcagctgg
    30541 agcggttctt tcaccgcaac ccaatatggt gcagcgtttc atcgccgcag ctccaccaat
    30601 gaaccacgcc tgatggtcag caccgatggc gtggcagata ttccggttca gggcaatctc
    30661 gactacacca accattttgg cattgcggtg gtgccgttga tttccagtta ccagccttcc
    30721 accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa cagttgcaga aaacgttatc
    30781 aaagaaacgt ggattgaagg cgcgataggt tacaaatcac tggcttcccg ttccggtaaa
    30841 gacgttaacg tcatcattcg caacgccagc ggtcagtttc ctcccctcgg agcggatatc
    30901 cgccaggatg acagcggcat tagcgtgggg atggttggcg aggaaggaca tgcctggtta
    30961 agcggagtcg ctgaaaatca aaagtttacc gtggtctggg gtgatagcca gcattgctcg
    31021 ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc tgattttacc ttgtcattaa
    31081 ttaaggaaat gaaaatgaca tttatgaaag gactacccct tttgttgttg gttgccagtc
    31141 tgtgcagcca cgctgcacta caacccgatc gcactcgtat tgtgtttaac gctaatgata
    31201 aagctaccag tctgcgggtg gataatcgta gcgataaact cccctacctc gcttattcct
    31261 ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct ggtcgccctg ccgccgatac
    31321 aacgtctgga accgaaagca acaacgcagg tacggatagt aaagcaagcc tcgaccacaa
    31381 aattaccggg cgatcgcgaa acgctatttt tctacaatat gcgtgaaatt cctcccgccc
    31441 cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat tcagagtcgt atcaaggtgt
    31501 tctggcgacc ggctgcatta cgcaagaaag caggagaaaa ggtcgaactg cagttacagg
    31561 tcagccagca gggcaaccaa ctgacactga aaaatcctac cgcgtattat ctgaccattg
    31621 cctacctggg acgaaatgaa aaaggcgttc tccccggctt caaaaccgtg atggttgcgc
    31681 cttttagtac ggtcaacaca aacaccggaa attatagcgg cagccagttt tatctcggtt
    31741 atatggatga ttacggcgcg ttacgcatga cgacgctcaa ctgcagcgga caatgccatt
    31801 tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga gtgttgctcg tcatctgtct
    31861 gttatttctg ccgttgaagt ctgcgcttgc cctgaactgc tactttggtt catccggtgg
    31921 ctcggtagaa aaatcagaag cgattcaacc ttttgctgta ccaggcaatg ccaaactcgg
    31981 cgataagatt tgggaatctg acgatattaa aattcccgtc tattgtgaca acaataccaa
    32041 cggcaatttt gaaagtgagc atgtttatgc ctgggtaaac ccctacccgg gggtgcagga
    32101 tcgctattat caactcggcg taacttacaa tggcgttgat tatgacgcca accagggaaa
    32161 aagccgcatc gacactaacc agtgtattga cagtaagaac attgacattt atacccctga
    32221 gcagattatc gcgatgggat ggcagaacaa aatttgctcg ggtgatcccg ctaatattca
    32281 catgtcgcgt acctttctcg ctcgcatgcg gttatacgtc aaaatacgag aaatgccgcc
    32341 gcatgattat caaagtacgc ttagcgacta tatcgtcgtg caatttgacg gtgccggtag
    32401 cgttaacgaa gaccctactg cccaaaacct gaaatatcat attactggtc tggaaaacat
    32461 tcgcgtgctg gattgcagcg tcaatttttc catttcaccg gaaacacagg tgattgattt
    32521 tggtaaattt aatttgctgg atattcgtcg ccacacaatg tcgaaaacat tcagtattaa
    32581 gacgacaaaa tcacaaaatg accaatgcac cgacggattt aaagtcagtt cctcctttta
    32641 taccgaagaa acgttggttg aagaagataa agcattgttg attggtaatg gtctgaagct
    32701 acgtttgctg gatgaaaacg cctcgcctta taccttcaat aaatatgctg aatacgccga
    32761 tttcaccagc gacatgttgg tctacgaaaa aacctataca gctgaacttt cgtccattgc
    32821 aggtaccccc atcgaggctg ggcccttcga tacagtggtg ctttttaaaa ttaactataa
    32881 ctgatttgcc atgatgccct ttgcagccgg aaagggcatc tctttcagaa aattgctgat
    32941 tttccccttt tccttgattg ctgttatttt ttcctggccc ggctttcttt atgctgtatc
    33001 gatacaccaa agagaaaata atgagggagc gaaggatgcc agttttgcag tggggaatgt
    33061 tatgtgtgct atcacttctc ctttctattg gtttcctcgc ggtacatctc ccggcggcgc
    33121 tattacttgg gccgatgatc gccgggatca tcttcagtat gcgcggaatt actctgcaac
    33181 ttccccgctc cgcttttctc gccgcgcagg ccattcttgg ctgcatgatt gcgcaaaacc
    33241 tcactggttc gattctcacc acgctggccg ccaactggcc gatcgtgctt gctattttac
    33301 tggtgacgct gctttccagc gccatcgtgg gttggttatt ggtgcgctat agctcactgc
    33361 ctggaaatac cggtgcctgg ggttcttctc caggcggcgc agcggcaatg gtcgctatgg
    33421 cgcaagatta cggcgcagat attcgcctgg ttgcatttat gcaatatctg cgagtgctgt
    33481 tcgtcgcggg ggccgctgtt ctggtcaccc gcatgatgct gggtgataac gctgaagcgg
    33541 ttaaccagca aatcgtctgg ttcccgccag tgagcattaa tctcctgctt acgattttgc
    33601 tcgcagtcgt tgccggtacg gcgggatgca tgctgcgtct tccttccggc acgatgctca
    33661 tcccgatgct ggcgggcgca gtgctccagt ctggtcagct catcaccatc gaactaccgg
    33721 aatggctgct ggcgatggcg tatatggcaa ttggctggcg gattggtctt ggtttcgata
    33781 aacaaatctt actgcgggca ctacgcccgc taccgcaaat cctgctgtcg atttttgctc
    33841 tgctggctat ttgtgcgggt atggcgtggg ggctgacccg gtttatgcac attgatttta
    33901 tgactgccta cctcgccacc agtcccggcg ggcttgatac cgtggcggtc atcgccgcag
    33961 ggagcaatgc cgatatggcg cttattatgg cgatgcaaac cctgcgcctg ttcagtattt
    34021 tgctgacggg gccagccatt gcgcggttta tttcaaccta tgcgccgaag cggtcggcct
    34081 agtgctggca gccagggcac cagtaaaacg ggcgagatga cagcgtggtt ttctcaatga
    34141 tgctgccaca gcgttcgcac ggttcgccat cacgatgaaa aaccttaaag cgaaacagcg
    34201 ccccatgatg cttattctca tccaccagcc ccctcgtagc gtaggaaaat cgaggaatat
    34261 ccagtaacgc gtgtgcgagt gcatccagtt gcgccgcatt gagatctttc gctttatgat
    34321 ttccagtcaa cccaacctgc cagaggatct ccacccgcaa ataattgcca agcccagcca
    34381 gaaacgcctg atcgagcagt aatcctgcaa actgacggtt acgaaagcgc ggtgacaata
    34441 accgttcctt taccacctcc ggcgtcagat tcggatccag cacatcggga ccaacgcgtt
    34501 gtaaaaacgg atgcgtggtc agttgttccg gcctcaacat ctcaatatcc gaggcgctat
    34561 aaagcagaat ggttttgtca gccgtttgca gttttacccg caatactcgc gtggtctgcg
    34621 gctcttcgcc ggtatcaacc acgcgccaga cgccgtaaag ctgattatgg ctgtagagcg
    34681 ttaagtcgtt ggaaaaatga gttaacaacg ccttaccacg cgtttccaca tgggtaacgt
    34741 gttgaccgat aagttgtgat tgataagttt ttaactgcgg gaaggcaaac cagacatcag
    34801 ttagtggttt gcctttgatc gccgcctcca ggttatccgc tgcacggcgg atctccgggc
    34861 cttcaggcat ggtgatatcc ttttttcatt ctttattaat cggggttatg ctgcgacaac
    34921 aatctccttt tcggcaaatg tagagcgtag tcggcgggcg aacgccagtg cgtgctcgcc
    34981 gtcgccatgc aggcagactg tttgcgccgt gaccgttgcc cattcgccgg tgatactttt
    35041 gactctgccg tgttgcacca tctctagcgt ttgcgccaac gcctgttctt cgttttcaat
    35101 caacgcgcct cgctgacttc gcggcaccag cgagccgtca gcctgataac cgcgatcggc
    35161 aaacacttcc tcgcgcgttg tcaggccata ttgcttgcct gcacgaatca gctcgcttcc
    35221 tgccagccca acgagaatca atgccggatc gcaagcgtat accgctctgg cgatggcgtc
    35281 tgccagttgt gcttctttcg ccgcctggtt gtacaacatg ccgtgcggtt tgacatgacg
    35341 cattacgccg ccttgcgcac gggcaatcgt tgccagcgcg ccaatttgat acagcgtctg
    35401 ggcgtaaacg gtttctggcg gcagctgcat ggcgctgcga ccaaaatttt ccctgtcggg
    35461 aaaactcggg tgagcgccaa tcgcgacacc attttttatt gcttcacgca cgcaagcctg
    35521 catgatttgc gcatcgcctg catgaaatcc acaggcaata ttggcagagg aaaccagcgt
    35581 taatagctct gcgtcgctgg cgcagccttc gcccagatcg gcgttcaggt caattttcat
    35641 tgtgcagccg ccacactaat tgttcgaaat aacgttgctg atcttgccgc gctttcagtg
    35701 cctcttccag tgaacactgg acaaaatgaa tcggctgacc gagcggaatt tgcgccagat
    35761 ggtacatatc agcctcaatg atacaggcaa tacgcgggta accatcggtg gtctgtgcgt
    35821 cgttcatcaa cacaatcggc tgcccgttat gtggcacctg caccacgccc ggtaacaaac
    35881 cgtgagataa cagttcgcga tcggtggtgc gttttaaaat ttgcccctgt aagcgatagc
    35941 ccatgcggtt actttgcgag ctaagctgcc agggcgaacg ccagaatgca tcctgcgagg
    36001 cgcgatcgaa ctcatgatat tccggccccg gcaaggcgcg aatgcggttg ccccacagca
    36061 gctgtttaac gccctgcgct tccataaaat cacgcttcga tttgccaatc gggagtcggt
    36121 caccatcctt cagtaaacgg ccttccagcc cgccaatccc cactttgaga tcggtgctgc
    36181 atgaccccat taccggcgga acatcaatac cacccgcgac cgccagataa ctgcgcatcc
    36241 cgtgctgcgg gcgtttaagc gttaaacgct ggcctgcttt catcggcaat cgccagccgg
    36301 tccagacggc attatcatcc agccgcgctt cgcaaccggc acccgtcaga gcaaaccacc
    36361 catcagtttc gaactcaacc gttaactgac cgagcgtgat ctccagcgcg ggggcattgg
    36421 cgtcattacc caccagcagg ttagcaatgc gtaacgcagg catatccagt gcgccgcagt
    36481 ggctgattcc cgactggcga aaaccgtgac gaccgccatc ctgcacagtg gtatacatgc
    36541 ccgcacgaat aatcttcagc atactccctc cttctgcggt acaaagcgca cgctgtctcc
    36601 cggacgtaat aagatgggtt cgtcacgcgc cggatcaaac aggctgagtg aggtatgacc
    36661 aatcaactgc cagccacccg gcgttgccag cggataaaca ccagtctgcg gcccgccgat
    36721 cccgacagaa cctgccggaa cgagcaagcg cggttcagcg cgccgtggcg tgtgtagttg
    36781 ttccggcaaa ctcccgagat acgggaagcc cggttgaaaa cctaaaaacc agaccacgta
    36841 ttccacggag gagtgcaatt caacaacctg tttttcgcta aacccgcaat gcgccgcgac
    36901 caccgccaaa tccggtccgc ctgcaccacc gtaaaccacc ggaatttcaa taaagcgaga
    36961 ctcaggttcc agggcctcgc tctcctccca ccagcgttgc aaacgctcta tggcatccag
    37021 cgccagcgac tcaggattac gcaaaatcac cgtgatattg ttcatgccgg gaatggcttc
    37081 aaccacattc ggcatatcca ccagacgctg cgccagtcgc cagatccgtt tctggctagc
    37141 cagcgtcacc ggcggttcca gttccagcac taccgccgtt tcacctatca gataacaacg
    37201 cgctcgttgc actttcgatc cctctgatta ttcgttatgc aggattagga atatcaataa
    37261 aggtcacatc aagatcggta ttttcattca gccactcgct caatgcgcga ataccaccac
    37321 gttcagtggc atggtgacct gcagcataaa aatgcaaccc ctgctcgcgg gctgaatgaa
    37381 tggtctgttc agaaacttcg ccagtaataa aagcatccac gccaaaacgc gcggcgctat
    37441 cgataaaact ttgcccgccg cccgtgcacc aggcgacgcg ctggaccacc tcaggtccgg
    37501 tatcgccaca ccataacggc ttacgtccca gacgcgcttc aatccaggag gctagttcca
    37561 gtcccggcac aggcatggtc agctctcccc acggcaccaa cggctcaatt tcgcccatga
    37621 ccgtgatccc cagtaacgcc gccagctgtg cgttattgcc cagctcagga tgtacgtcaa
    37681 gcggcaaatg ccagccatac aggttgatat cattcgccag caacgttttt aaacggttac
    37741 gcttcattcc gcgaatgacc ggagactctc ctttccagaa gtagccatga tgcacaatga
    37801 ccgcatccgc gcccagacgc actgcctcat cgagcaaagc ctggctggcg gtgacaccgg
    37861 taacaatttt ttgcaccgtc tctttgcctt ctacctgcaa accattcggc gcgtaatcac
    37921 taatcgccgc gctgttcagt ttttcgttaa tcagttgttc cagttcggtg tttttcatct
    37981 tcgcgccttc cgtattctga tagctacacc gttaacatag cgttacctcg cctgttcgtc
    38041 gatagccaat cttttgacat attaagaaac aaacccgacg ccaaaagacg caaattgttt
    38101 atatcatcac gcttcagcta atgtatattt atgcaatcag acagcatatt gcataaataa
    38161 tcagatctgt atcaaagttt taactttaag gaaacacaag cacgccgcgc aactattacg
    38221 tgacgttgca gtcaattgtg gaattattat gaataaacac gcatcacaac cgcgcactat
    38281 ttactatgtc gttgcgctgc aaatctggga atattttagc ttttacggca tgcgtgccct
    38341 gctgattctc tatctcacca atcaactaaa atacaacgat actcacgcct acgagttatt
    38401 tagcgcctac tgttcgctgg tgtatgtcac gccaatcctc ggtggctttt tggcggataa
    38461 agttctcggc aatcgcatgg cggtgatgct gggggcgttg ttgatggcga tcggtcatgt
    38521 ggtgctgggt gccagtgaga tccatccgtc attcctctat ctgtccctgg cgattatcgt
    38581 ttgtggctat ggtctgttta aatccaatgt cagttgcctg ctcggcgagc tgtatgagcc
    38641 aaccgatccg cgtcgtgatg gcggtttctc gctgatgtat gcagcgggta acgtggggtc
    38701 tattatcgca cctatcgcct gtggttacgc ccaggaagag tacagctggg cgatgggctt
    38761 tggcctggcg gcggtgggta tgatcgcggg tctggtcatt ttcttatgtg gcaatcgtca
    38821 tttcacacat acccgcggcg ttaataaaaa agtactgcgt gcaacaaact ttctcctgcc
    38881 taactgggga tggctgctgg ttctgctggt ggcaacgcct gcgctgatta ccgtactgtt
    38941 ctggaaagag tggtcggtat acgccttaat tgtcgcgact attattggcc tgggtgtact
    39001 ggcaaaaatt tatcgcaaag cagaaaacca gaaacagcgt aaggagctgg ggctgattgt
    39061 gacgctcacc ttcttcagta tgttgttctg ggccttcgca caacagggcg gcagctcaat
    39121 tagcctttat atcgaccgct tcgttaaccg cgatatgttt ggttataccg ttccgactgc
    39181 tatgttccag tcgattaatg ccttcgcagt tatgctatgc ggtgtgttcc tggcgtgggt
    39241 ggttaaagaa agcgtcgcag gtaatcgtac cgtgcgcatc tggggaaaat ttgctcttgg
    39301 ccttggcctg atgagcgccg gattctgcat tctgacctta agcgcccgct ggtccgcaat
    39361 gtatggtcac tcttctctgc cactgatggt gttaggcctc gcggtgatgg gctttgcgga
    39421 actgtttatc gacccggttg ccatgtcgca aattacgcgc attgaaattc cgggcgtgac
    39481 cggcgtatta accggcatct acatgctgct ttctggcgcg atcgcgaact atctggcggg
    39541 cgtgattgcc gatcagacat cgcaggcttc gtttgatgct tccggggcga tcaactactc
    39601 catcaatgca tatattgaag tatttgatca aattacctgg ggcgcactgg cgtgtgtagg
    39661 actggtactg atgatttggc tgtatcaggc gctgaaattc agaaaccgcg cgctggcgct
    39721 ggagtcataa taggccagca ccgatgcctg atgcgacgct tgacgcgtct tatca
//
LOCUS       DAJTVK010000023        39570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000023 DAJTVK010000000
VERSION     DAJTVK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.023"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="OO372_001285"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="OO372_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6340830.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="OO372_001286"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="OO372_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6340831.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="OO372_001287"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="OO372_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6340832.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="OO372_001288"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="OO372_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6340833.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="OO372_001289"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="OO372_001289"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6340834.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="OO372_001290"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="OO372_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6340835.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="OO372_001291"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="OO372_001291"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6340836.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="OO372_001292"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="OO372_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6340837.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="OO372_001293"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="OO372_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6340838.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="OO372_001294"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="OO372_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR6340839.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="OO372_001295"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="OO372_001295"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6340840.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="OO372_001296"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="OO372_001296"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6340841.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="OO372_001297"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="OO372_001297"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6340842.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="OO372_001298"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="OO372_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6340843.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="OO372_001299"
     CDS             complement(14282..14581)
                     /locus_tag="OO372_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6340844.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="OO372_001300"
     CDS             complement(14793..15233)
                     /locus_tag="OO372_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6340845.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="OO372_001301"
     CDS             15334..15933
                     /locus_tag="OO372_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6340846.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="OO372_001302"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="OO372_001302"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6340847.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="OO372_001303"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="OO372_001303"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6340848.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="OO372_001304"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="OO372_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6340849.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="OO372_001305"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="OO372_001305"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6340850.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="OO372_001306"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="OO372_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6340851.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="OO372_001307"
     CDS             complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="OO372_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6340852.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21877..22575
                     /gene="cpxR"
                     /locus_tag="OO372_001308"
     CDS             21877..22575
                     /gene="cpxR"
                     /locus_tag="OO372_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6340853.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22572..23945
                     /gene="cpxA"
                     /locus_tag="OO372_001309"
     CDS             22572..23945
                     /gene="cpxA"
                     /locus_tag="OO372_001309"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6340854.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="OO372_001310"
     CDS             complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="OO372_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6340855.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="OO372_001311"
     CDS             complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="OO372_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR6340856.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="OO372_001312"
     CDS             complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="OO372_001312"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6340857.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27022..28056
                     /gene="rhaT"
                     /locus_tag="OO372_001313"
     CDS             27022..28056
                     /gene="rhaT"
                     /locus_tag="OO372_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR6340858.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGAGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="OO372_001314"
                     /pseudo
     CDS             complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="OO372_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="OO372_001315"
     CDS             complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="OO372_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6340859.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30099..31568
                     /gene="rhaB"
                     /locus_tag="OO372_001316"
     CDS             30099..31568
                     /gene="rhaB"
                     /locus_tag="OO372_001316"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6340860.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31565..32824
                     /gene="rhaA"
                     /locus_tag="OO372_001317"
     CDS             31565..32824
                     /gene="rhaA"
                     /locus_tag="OO372_001317"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6340861.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33066..33890
                     /gene="rhaD"
                     /locus_tag="OO372_001318"
     CDS             33066..33890
                     /gene="rhaD"
                     /locus_tag="OO372_001318"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR6340862.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33900..34214
                     /gene="rhaM"
                     /locus_tag="OO372_001319"
     CDS             33900..34214
                     /gene="rhaM"
                     /locus_tag="OO372_001319"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6340863.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34515..34961
                     /locus_tag="OO372_001320"
     CDS             34515..34961
                     /locus_tag="OO372_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6340864.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            34972..36423
                     /locus_tag="OO372_001321"
     CDS             34972..36423
                     /locus_tag="OO372_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6340865.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36413..37483
                     /locus_tag="OO372_001322"
     CDS             36413..37483
                     /locus_tag="OO372_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6340866.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37483..39231
                     /locus_tag="OO372_001323"
     CDS             37483..39231
                     /locus_tag="OO372_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR6340867.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39280..>39570)
                     /locus_tag="OO372_001324"
     CDS             complement(39280..>39570)
                     /locus_tag="OO372_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR6340868.1"
                     /translation="KFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLET
                     KKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9616 a        10282 c        10274 g         9398 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacagcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgccca
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agctaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccacgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactccca ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg
    21181 tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg
    21241 agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca
    21301 actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca
    21361 ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg
    21421 ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc
    21481 gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca
    21541 taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc
    21601 tctgcgtact gcgctgcgta agttcttcac ccggatgcca gttatcgcct gaaccgactt
    21661 cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc catgacggca gcggtaacta
    21721 tgcgcatcat ttgctcccaa aatctttctg tcgcgattca acgatagaga gtttacgatt
    21781 caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc
    21841 tgatgacgta atttctgcct cggaggtatt taaacaatga ataaaatcct gttagttgat
    21901 gatgaccgag agctgacttc cctattaaag gagctgctcg agatggaagg cttcaacgtg
    21961 attgttgccc acgatgggga acaggcgctt gatcttctgg acgacagcat tgatttactt
    22021 ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc acttcgccag
    22081 acacaccaga cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc
    22141 ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg
    22201 gcacgtattc gcgcgatcct gcgccgttcg cactggagcg agcaacagca aaacaacgac
    22261 aacggttcac cgacactgga agttgatgcc ttagtgctga atccaggccg tcaggaagcc
    22321 agcttcgacg ggcaaacgct ggagttaacc ggtactgagt ttaccctgct ctatttgctg
    22381 gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa
    22441 cgcctgacgc ctttcgaccg cgctattgat atgcacattt ccaacctgcg tcgtaaactg
    22501 ccggatcgta aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg
    22561 gtttctgctt catgataggc agcttaaccg cgcgcatctt cgccatcttc tggctgacgc
    22621 tggcgctggt gttgatgttg gttttgatgt tacccaagct cgattcacgc cagatgaccg
    22681 agcttctgga tagcgaacag cgtcaggggc tgatgattga gcagcatgtc gaagcggagc
    22741 tggcgaacga tccgcccaac gatttaatgt ggtggcggcg tctgttccgg gcgattgata
    22801 agtgggcacc gccaggacag cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg
    22861 ctgaacgcag cgaaatgcag atcattcgta actttattgg tcaggccgat aacgccgatc
    22921 atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg
    22981 gcgaagataa ttaccaactt tatctgattc gtccggccag cagttctcaa tccgatttca
    23041 ttaacttact gtttgaccgc ccgctattac tgctgattgt caccatgttg gtcagtacgc
    23101 cgctgctgtt gtggttggcc tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg
    23161 ccgatgaagt tgcccaggga aacttacgcc agcacccgga actggaagcg gggccacagg
    23221 aattccttgc cgcaggtgcc agttttaacc agatggtcac cgcgctggag cgcatgatga
    23281 cctctcagca gcgtctgctt tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc
    23341 tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa ctcgagcgta
    23401 ttgaaaccga agcgcaacgt ctggacagca tgatcaacga tctgttggtg atgtcacgta
    23461 atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg tggagtgaag
    23521 tgctggataa cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc
    23581 cgcctgggcc gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca
    23641 ttgttcgtaa tgctctgcgt tattcccata cgaagattga agtgggcttt gcggtagata
    23701 aagacggtat caccattacg gtggacgacg atggtcctgg cgttagcccg gaagatcgcg
    23761 aacagatttt ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa tctggcggta
    23821 caggattggg gctggcgatt gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg
    23881 cagaagacag cccgctgggc ggtttacggc tggtgatttg gttgccgctg tataagcgga
    23941 gttaaactcc gcatttgtag gcaggataag gcgtttacgc cgcatccggc atttgagcag
    24001 gatgcctgat gcgacgctga tagcgtctta tcaggcctac actccggggg ttattttccc
    24061 cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc
    24121 gtccagcttt tcgataaccc ggcggcggag agtaaacggt gatactgatc gtcatcaaac
    24181 ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca catcgctgac acgggaaacc
    24241 aactccagcg gcgcgtccgc agacactttc cctggcgcta tcacgctata caaccacccc
    24301 accttacctg tgttttgcat caactgcgct atatcgctga tatcaaaatg gtaattgagt
    24361 ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg cctctcccca gcggaaaata
    24421 tcgcccatat gaacattact ttccgtgaga ccgtcggttg agagattttc gccaaacgca
    24481 ggcgcaacaa acaactccgc ctgttccgga aattcccgca cccagtagag atagtgctca
    24541 cgaggataat gacacagcgc tctgtctggc ccgccgtgaa cctttttctc cgcctgctcg
    24601 tcaccttcca gccccagctc cgtcagcatc aactcaccat caacctggat tttggcaatt
    24661 gcgctgggtt tgccttcggg ataagcctga atcttgcctg tgtatacatc aaccggatat
    24721 cgcatctgtt ccctctccac gagaaatttc cccattagac catatttatt ttatgtcagg
    24781 cgtgctgttt tcgcggtaag tatccaggca ggaactcaat agcaaaccgg acgaacgtta
    24841 gttcatccgg ctgtggggat taaggaagca ggtttatttc accgtaccta aaatttcgat
    24901 tttcgctgct ctggctttga ctttacgtga ccagatagag gtaagaattg gcaccagaat
    24961 cgaagtcaca atgaccgccg tcgctaccag cgaagttgct gccggagcca tcggtttaaa
    25021 cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc gcggcccctg cggaactgga
    25081 agcggcaatt ccggcagtgc catcgccacc gccaatcaat ttatcggcga taatcaacgg
    25141 aataccggtc acgataatta ctgccacacc caacaggatc cccagcaaac cagtctgggc
    25201 aatcacagtc aaatcaatgg tattgcccag cgcgaaggca aagaatggaa tcagcgtttg
    25261 cactgctttg ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg caaagcccac
    25321 caggaacggt aatactgcgc cgacgaaaac atgcggttca aacgaggcaa tcccggcagt
    25381 gcccagaata atcatcgtca tgagcggccc ggactccaac gacatcaaca caaatgcccc
    25441 agcttcttct ttcgtaccgt actgctgcat gatggaagcg taaagtccgc cgttggtcat
    25501 atccatcgcc gccaccagcg ccagcgttga aagtccggca aagaatccaa cttcaacacc
    25561 atgttccgga ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg caattttagt
    25621 taccaccagc gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc
    25681 ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta ccggtaatca tcccgttggt
    25741 gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa
    25801 tagcgggacg agcatcatcc cccccgggat tttctcaatc gagcgtttta tctgcatttc
    25861 catcacctgt cggcatattt ttaagggctg atgcgtaatt cctcaatgga gcatcagtgt
    25921 tgatggaaac gaagatagac attcatttaa tcaataaaag tgatcaaaac cacatcaatt
    25981 gaaacgctgt tttattttta tcggatcatt gtttttattt tgaaattgat cacaaaaaaa
    26041 ccaccgccgt tggcgatggt tcatttttta agctgatatg cggcctatac gcctcattgc
    26101 agcaggcggc aaatgattat tttttcgccg caaaacgtgc cgctgcttcg tcccagttca
    26161 ccacgttcca gaactcttta atgtagtccg gacggcggtt ctggaatttc aggtagtaag
    26221 catgttccca cacatccagg cccagaatcg ggaagccgga agcgccagaa atagcttcac
    26281 ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc cagtttatcg cctttcagca
    26341 ccagccatgc ccagccggaa ccaaagcggg aagctgccgc tttttcaaat tctgctttga
    26401 agttatcaac ggagccgaag tcacgttcga tagccgcttt caggtcaccc tgcagggtgg
    26461 tgcctttttt cagacctttc cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc
    26521 gcagtacggt tttcttgtct gctggcagct ggtccagttt ggtgatcagc tcttcaaccg
    26581 gcaggttggc aaattctggc aggctttcca gcgccgcgtt ggcgttgttt acgtaggtct
    26641 gatggtgttt ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt tccagggcat
    26701 cgtaagcata cggcagggat ggcagggtat agctcatatt catctccagt attgtcgggc
    26761 ggccgattgt taatgccgcg taagcagttg gttcattata gttaattaaa tgatattgaa
    26821 aatgattatc aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat
    26881 gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa atcgacaacg gcggcaacag
    26941 gcgaaaggtt aatcgacagc acgattttta cactcatctc gtcggagatg tgaagcgacg
    27001 aaaaatgatg aggataagaa gatgagtaac gcgattacga tggggatatt ttggcatttg
    27061 atcggcgcgg ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa aaaatggtca
    27121 tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc gtggaccatc
    27181 agcgccctgt tactaccgga tttctgggcg tattacagct cgtttagtct ctctacgctg
    27241 ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta atatcaacta tggcctgacc
    27301 atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca ttggcattac gttgattgtc
    27361 ggtacgctga tgacgccaat tatcaacggc aatttcgatg tgctgattaa caccgaaggc
    27421 ggacgcatga cgttgctcgg cgttctggtg gcgctgattg gcgcagggat tgtaactcga
    27481 gccgggcagt tgaaagagcg caagatgggc attaaagccg aagagttcaa tctgaaaaaa
    27541 gggctggtgc tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac
    27601 gccgcaaaac cgatgcatga agccgctgcc gcacttggcg tcgatccact gtatgtcgct
    27661 ctgccaagct atgttgtcat catgggcggc ggcgcgatca ttaacctcgg tttctgtttt
    27721 attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg
    27781 ctgatcattc acaatgtgtt actctcggca ctgggcgggt tgatgtggta tctgcaattc
    27841 tttttctatg cctggggcca cgcccgcatt ccggcgcagt atgactacat cagttggatg
    27901 ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg
    27961 aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc
    28021 gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt gagatgacgc
    28081 cactggctgg gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata gttactatct
    28141 tcaaagccac attcggtcga aatatcactg attaacaggc ggctatgctg gagaagatat
    28201 tgcgcatgac acactctgac ctgtcgcaga tattgattga tggtcattcc agtctgctgg
    28261 cgaaattgct gacgcaaaac gcgctcactg cacgatgcct catcacaaaa tttatccagc
    28321 gcaaagggac ttttcaggct agccgccaac cgggtaatca gcttatccag caacgtttcg
    28381 ctggatgttg gcggcaacga atcactggtg taacgatggc gattcagcaa catcaccaac
    28441 tgcccgaaca gcaactcagc catttcgtta gcaaacgaca catgctgact actttcatgc
    28501 tcaagctgac cgataacctg ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt
    28561 ggttgccctg cgctggcgct aaatcccgga atcgctccct gtcagtcaag attcagcttc
    28621 agccgctccg ggcaataaat aatattctgc aaaaccagat cgttaacgga agcgtaggag
    28681 tgtttatcgt cagcatgaat ataaaagaga tcgccacggg taatgcgata aggacgatcg
    28741 ttgagtacat gcagaccatt accgcgccag acaatcacca gctcacaaaa atcatgtgta
    28801 tgttcggcaa agacatcttg cggataacgg tcagccacag cgactgcctg ctggtcgctg
    28861 gcaaaaaaat catctttgag aagttttaac tgatgcgcca ccgtggctac ctcggccaga
    28921 gaacgaagtt gattattcgc aatatggcgt acaaatacgt tgagaagatt cgcgttattg
    28981 cagaaagcca tcccgtccct ggcgaatatc acgcggtgac cagttaaact ctcggcgaaa
    29041 aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga taggcgatgt cagtaacgct
    29101 ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg
    29161 ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga
    29221 aaattgatcc gccacggcat cccaattcac ctcatcggca aaatggtcct ccagccaggc
    29281 cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg
    29341 cagcaagagc agtaattgca taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt
    29401 ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc acctgctgca atacgctgtg
    29461 gttaacgcgc cagtgagacg gatactgccc atccagctct tgtggcagca actgattcag
    29521 cccggcgaga aactgaaatc gatccggcga gcgatacagc acattggtca gacacagatt
    29581 atcggtatgt tcatacagat gccgatcatg agcgcgtacg aaacagaccg tgccaccggt
    29641 gatggtatag ggctgcccat taaacacatg aatacccgtg ccatgttcga caatcacaat
    29701 ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc gggagccggg gttctatcgc
    29761 cacggacgcg ttaccagacg gaaaaaaatc cacactatgt aatacggtca tactggcctc
    29821 ctgatgtcgt caacacggcg aaatagtaat cacgaggtca ggttcttacc ttaaattttc
    29881 gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat
    29941 gcggtgagca tcacatcacc acaatgaagc taattgtgaa catcatcacg ttcatctttc
    30001 cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt
    30061 tttagactgg tcgtaatgaa attcagcagg atcacattat gacctttcgc aattgtgtcg
    30121 ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc
    30181 gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc cagaacggtt
    30241 atgtcacctg gaatgtggat agcctggaaa gtgccattcg ccttggatta aacaaggtgt
    30301 gcgaggaagg gattcgtatc gatagcattg ggattgatac ctggggcgtg gactttgtgc
    30361 tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat agccgctcca
    30421 atggcctaat ggcgcaggca cagcaacaac tcggcaaacg cgatatttat caacgtagcg
    30481 gcatccagtt tctgcccttc aatacgcttt atcaactgcg tgcgctgacg gagcaacaac
    30541 ctgaacttat tccacacatt gctcacgctc tgctgatgcc ggattacttc agctatcgcc
    30601 tgaccggcaa gatgaactgg gagtacacca acgccacgac cacgcaactg gtcaatatca
    30661 atagcgacga ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa gcctggtttg
    30721 gtcgcccgac gcatccgggt aatgtcattg ggcactggat ttgcccgcag ggtaatgaga
    30781 ttccggtggt cgccgttgcc agccatgata ccgccagcgc ggttatcgcc tcgccgttaa
    30841 acggttcacg cgctgcttat ctctcttctg gcacctggtc attgatgggc ttcgaaagcc
    30901 agacgccatt taccaatgac acggcgctgg cagccaacat aaccaatgaa ggcggggcgg
    30961 aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg gctgcttcag cgagtgcttc
    31021 aggagcggca aatcaacgat ctcccggcgc ttatcgccgc gacacaggca cttccggcct
    31081 gccgcttcat catcaatccc aatgacgatc gctttattaa tcctgaggcg atgtgcagcg
    31141 aaattcaggc tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat gctgaactgg
    31201 cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc
    31261 agctacgcgg tgaagatttc tcgcaactgc atattgtcgg cggcggctgc cagaacacgc
    31321 tgctcaacca gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg cctgttgaag
    31381 cctcgacgct cggcaatatc ggcatccagt taatgacgct ggatgaactc aacaatgtgg
    31441 atgatttccg tcaggtcgtc agcaccaccg cgaatctgac cacctttacc cctaatcctg
    31501 acagtgaaat tgcccactat gtggcgcagc ttcactcgac acgacagaca aaggagcttt
    31561 gcgcatgacc actcaactgg aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt
    31621 ggggattgat gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt caatgcactg
    31681 ctggcagggc gatgatgttt ccggttttga aaacccggaa ggttcgctga ccggtgggat
    31741 tcaggctatt ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac gagccgatct
    31801 ggaacaggct atgcggctga ttccggggcc gaaacggctt aatttacatg ccatctatct
    31861 ggaatcagac acgccagtct cgcgcgacca gatcaaacct gagcacttca aaaactgggt
    31921 tgaatgggcg aaagccaatc agctcggtct ggattttaac ccctcctgtt tctcgcatcc
    31981 gctaagcgcc gatggcttta cgctctcgca tcccgacgac agcattcgcc agttctggat
    32041 tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc
    32101 atcggtgatg aacatctgga tcccggatgg tatgaaagat atcaccgttg accgtctcgc
    32161 cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc agcgagaagc tggatccggc
    32221 gcaccatatc gacgccgttg agagcaaatt gtttggcatt ggcgcagaga gctacacggt
    32281 tggctccaat gagttttaca tggggtatgc caccagccgc cagactgcgc tgtgcctgga
    32341 cgccgggcat ttccacccga ctgaagtgat ttccgacaag atttccgccg ccatgctgta
    32401 tgtaccgcag ttgctgctgc acgtcagccg tccggttcgc tgggacagcg atcacgtggt
    32461 gctgctggat gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg atctgttcga
    32521 ccgggtgcat atcggcctcg acttctttga tgcctctatc aaccgcattg ccgcgtgggt
    32581 cattggtaca cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga
    32641 gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac tggaagaaca
    32701 gaaatcgttg ccgtggcagg cggtctggga aatgtattgc caacgtcacg atacgccagc
    32761 aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg
    32821 gtaaacactg ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga atgaccatgc
    32881 gtgctgccac cctcaccccg gccctctccc tgaaagggcg agggagaaaa acatagcctc
    32941 gtgcggcaac ggagctgaac accgctccgg acagtcccct cgcccctacg gggagagggt
    33001 tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc aacctaattt acaggaacac
    33061 agaacatgca aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg
    33121 atgcctggct gaaaggctgg gacgagcgca atggtggtaa cctgacgctg cgcctggatg
    33181 acgccgatat cgcgccttat cacggcaact ttcatgcaca accgcgctat attcccctca
    33241 gccaacccat gcctttactg gcaaatacgc cgtttattgt caccggctcc ggcaaattct
    33301 tccgtaacgt ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg
    33361 gcgcgggcta ccatattctc tgggggttaa ccaacgaagc cgttccaact tccgaacttc
    33421 cggctcactt cctttcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga
    33481 tcatgcactg ccacgccacc aacctgatcg ccctcaccta tgtacttgaa aacgacaccg
    33541 cggtctttac tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg
    33601 gcgttggcat tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac
    33661 aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt
    33721 cgacgttgga tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag
    33781 tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc
    33841 tcggcaagcg cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa ggagcaaaca
    33901 tgatccgcaa agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc
    33961 ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact
    34021 acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg
    34081 aagaacggtg gaatgcggta gccagcactg atgtttgcca acgttggtgg aaatatatga
    34141 ccgatgttat gcccgctaac ccggataaca gcccggtgag tagcgagctg caagaagtgt
    34201 tttacctgcc gtaattgccc aatgtgccag agatgcctcc ggcacaatat tttaaatttc
    34261 gatttgaaaa attaaacata ttacatctcc cggtaagtta tatttccctg atacattgtg
    34321 agtaaatcac aaaaataatg aataacccat taatgattca tgtggtttat ttaaataacc
    34381 attatgtgca ttactccgca aatctgacct ttcactctgt ctcaaattgt cattgtttac
    34441 cgacatatcg cctctcatca cttaatcgct ccaccattac aattgattaa aatatatttt
    34501 atggagtccg attaatggca gctcttactg caagctgtat tgacctgaat attcagggca
    34561 atggcgctta ttccgttctg aagcagttgg cgacaatagc gttacaaaac ggttttatca
    34621 ccgactcaca ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac tctactggat
    34681 ttggttccgg tgtcgccgtg ccgcacggta aaagcgcctg cgttaaacaa ccgtttgtat
    34741 tattcgcccg caaagcgcag gctattgact ggaaagccag cgatggcgaa gacgtcaatt
    34801 gctggatctg cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa atcatcggca
    34861 cactgtgtcg caaaattatc caccaggatt ttattcatca actgcaacag ggcgacgccg
    34921 accaggtgct tgccttgtta aatcaaaccc tcagctcata aggaagtggc gatggagtca
    34981 tccttatgta ttgtcgcgat caccaactgc cccgccggga tcgctcacac ctacatggtg
    35041 gcggaagccc tggaacagaa agcccgttct ctcggccata ccataaaagt ggaaactcaa
    35101 gggtccagtg gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc cgattacgtc
    35161 attctcgcta ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc cgggaagaaa
    35221 gtttatgaga ttgccatctc ccaggcgttg aaaaatatcg accagatttt cagcgaatta
    35281 ccgacaaact cgcagctttt tgccgcagat agcggcgtga agcttggcaa acaggaagtg
    35341 caaagcggca gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct gccgtttgtc
    35401 atcggcggtg gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg tttgccgtat
    35461 accgatatgt cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat cggttacctt
    35521 ggcttcacct ttatgatccc catcatgggc gcttatattg cctcgtcgat tgccgataaa
    35581 ccggctttcg ctccggcgtt tctggtttgc tatctggcga acgacaaagc gctgctcggc
    35641 acccagtcgg gtgcgggatt cctcggcgcg gtggtgctgg ggctggcgat tggctatttc
    35701 gtcttctggt tccgcaaagt gcgccttggc aaagcattac aaccgctgct cggttcaatg
    35761 ctgatcccgt tcgttactct gctggttttc ggcgtgctca cttactacgt tatcgggccg
    35821 gtgatgtccg acctcatggg cgggctgctc cacttcctga ataccattcc tccatcaatg
    35881 aagtttgcgg cagcgtttct ggtcggtgcg atgctggcgt tcgatatggg cggccccatc
    35941 aataaaaccg cctggttctt ctgcttctca ctgctggaaa aacacattta tgactggtac
    36001 gccatcgtcg gcgttgtcgc gttgatgccg cccgttgcgg cgggtcttgc gacgttcatt
    36061 gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca gtagtgccat tgtggtcggt
    36121 gccacagtcg caaccgaacc ggctatacct tacgctctgg cagctccgtt gccgatgatt
    36181 accgccaata cgctggcggg tggcattacc ggcgtgctgg tgatcgcttt cggaatcaaa
    36241 cgtctggcac ctggtttggg tatctttgac ccgctgatcg gcctgatgtc gccggtgggt
    36301 tcgttttatc tggtgctagc gattggcctc gcgctgaaca tttcattcat tatcgtcctg
    36361 aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac aggagcttgt ccatgaacat
    36421 tgagttactg caacagttgt gcgaagccag cgccgtcagc ggcgatgaac aggaagttcg
    36481 cgacattctg ataaacacgc tggaaccttg cgttaatgag atcacctttg atggtctggg
    36541 cagctttgtt gcccgtaagg ggaataaagg tccaaaagtt gccgttgtca ggcatatgga
    36601 tgaagtcggc tttatggtca cccacatcga cgagagcggt tttctgcgct ttaccaccat
    36661 tggcggctgg tggaatcagt caatgctcaa ccaccgggta accatacgca cacacaaggg
    36721 atttaaaatc cctggtgtga ttggttccgt cgcacctcat gcgttaacgg aaaagcaaaa
    36781 gcaacagccg ctgtcatttg atgagatgtt cattgatatt ggcgcgaaca gtcgcgatga
    36841 ggtggaaaaa cgcggtgtgg cgatgggcga ttttattagc ccggaagcca attttgcctg
    36901 ctggggcgaa gataaagtgg tcggcaaggc gctggataac cgcatcggct gcgcaatgat
    36961 ggctgaacta ttgcagacgg tgaataatcc cgaaattacg ctgtatggcg tcggcagcgt
    37021 ggaagaagaa gttgggctac gcggggcaca aacctcggcg gaacacatta aaccggatgt
    37081 ggtgattgtg ctggataccg ctgtcgcagg tgatgttccg ggcattgata acattaaata
    37141 cccgctgaaa ctgggccagg ggccggggct gatgctgttt gacaagcgct acttccccaa
    37201 ccagaaactg gtggcggcgt taaaaagctg tgccgcacat aacgatttac cgctgcaatt
    37261 ttccaccatg aaaaccggtg cgacggatgg cgggcgctac aacgtgatgg gcggcgggcg
    37321 tccggttgtt gcgctgtgcc tgccaacccg ctatctgcac gctaacagcg gcatgatttc
    37381 aaaagccgat tacgatgctc tgctcacgtt gatacgggat tttctgacca ccttaaccgc
    37441 ggagaaagtc aacgcgttta gccagttccg tcaggtggat taatgctaaa cgaacgccag
    37501 ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc ctggtgagct ggcgcaacag
    37561 actggcgttt caggcaggac catcctgcgt gatattgact atctcaactt tacccttaac
    37621 ggcaaagccc gcattttcgc cagtggcagt gcgggctatc agctggaaat cttcgagcgc
    37681 cgcagctttt ttcagttgct gcaaaagcac gataacgacg atcggctgct ggcgctgtta
    37741 ttactgaata cctttactcc ccgtgcgcaa ctcgcctcgg cgcttaattt gccagaaacc
    37801 tgggtagcag agcgtctgcc ccggttaaaa cagcgttatg aacgcacttg ttgcctggcc
    37861 agtcgccccg gtttgggcca tttcattgat gagacagaag agaaacgcgt tatcttgctg
    37921 gcgaacttgc tgcgcaaaga tccgttttta attccgctgg cgggcgtaac acgagacaat
    37981 tttcagcatt tatccacggc ctgcgacaac caacaccgct ggccgctcat gcagggtgat
    38041 tatctctcca gcctgattct ggcgatttac gccctgcgta atcaactgac cgatgagtgg
    38101 ccgcaatatc ccggtaacga gataaaacaa atcgttgaac agagcggcat gtttcttggt
    38161 gataacgctg taagaaccct gacaggtttg atagagaaac agcatcagca agcgcaggta
    38221 atttcagccg atcatgtgct ggggttgctg caaagggtgc caggcatcgc gtcattgaat
    38281 attattgata cgcagctggt tgagaatatt accgggcatt tattacgttg ccttgccgca
    38341 ccggtgtgga ttgccgagca ccgccaaagc agcatgaata acctgaaagc cgcctggcct
    38401 gcggcctttg atatgagtct gcactttatt acgctactgc gtgaacagct cgatattccc
    38461 cttttcgata gcgatctgat cggtttgtat tttgcctgtg cgctggaacg gcatcaaaac
    38521 gaacgccagc cgatcatttt gctctcggac cagaacgcga ttgccactat taatcagctc
    38581 gccattgagc gcgatgtttt acattgtcgg gtgattattg cccgtagctt aagcgagctt
    38641 attgccattc gcgaagagat tgagccgtta ttgatcatta acaacagcca ttatttactg
    38701 gatgacgcgg taaataatta catcaccgta aaaaatatca ttacggctgc cgatatcgaa
    38761 caaataaaac actttctggc gacggcattt attcgccaac agccggaacg ctttttctct
    38821 gcccccggaa gttttcatta ttcgaatgta cgcggtgaaa gctggcaaca tattacccgg
    38881 caaatttgtg cgcaattagt ggcacaacac catattaccg ccgatgaagc gcaacggatc
    38941 atcgcccgcg aaggcgaagg tgaaaacctg attgttaatc gcctcgcaat cccacattgc
    39001 tggagcgaac aggagcgacg ttttcgtgga ttttttatta ccctcgccca accagttgag
    39061 gtgaataacg aagtcattaa ccatgtcttg atcgcctgcg ccgccgccga tgcgcgtcat
    39121 gagctaaaaa tatttagcta tctggcaagc gtattgtgtc agcatccggc agaagttatt
    39181 gccgggttaa caggatatga ggcatttatg gagttacttc acaaggggtg aactgtgtcg
    39241 gatgcgccgc atccgacact gcattttcat caaaaacgtt taacgcaggc tattatagtc
    39301 atcaaccatt tcgttgtact cgcgtaacgc tcgtggaaat gagtcttcga ccatccagct
    39361 tgtgtttgca tcctgaagat gctctttatc ccagtcagag tacgggactt tatctctgac
    39421 gcggcgaaca tattttttag tctcgagttg atactggtct gccgaattaa tgaacgagaa
    39481 attcataccg cctttatccg cttctttggc ctgcgctacc agcgtttcca gttcagagac
    39541 tttcttcatt gccgcatcaa catcaaactt
//
LOCUS       DAJTVK010000024        39477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000024 DAJTVK010000000
VERSION     DAJTVK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.024"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO372_001325"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO372_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6340869.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OO372_001326"
     CDS             1946..2230
                     /locus_tag="OO372_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6340870.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OO372_001327"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OO372_001327"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6340871.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OO372_001328"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OO372_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6340872.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OO372_001329"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OO372_001329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6340873.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7236..7799
                     /locus_tag="OO372_001330"
     CDS             7236..7799
                     /locus_tag="OO372_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6340874.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7881..10523
                     /gene="yfcU"
                     /locus_tag="OO372_001331"
     CDS             7881..10523
                     /gene="yfcU"
                     /locus_tag="OO372_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR6340875.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            10543..11294
                     /locus_tag="OO372_001332"
                     /pseudo
     CDS             10543..11294
                     /locus_tag="OO372_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            11269..11817
                     /locus_tag="OO372_001333"
     CDS             11269..11817
                     /locus_tag="OO372_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6340876.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            11814..12284
                     /locus_tag="OO372_001334"
     CDS             11814..12284
                     /locus_tag="OO372_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6340877.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            12281..12805
                     /locus_tag="OO372_001335"
     CDS             12281..12805
                     /locus_tag="OO372_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6340878.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            12792..13664
                     /locus_tag="OO372_001336"
     CDS             12792..13664
                     /locus_tag="OO372_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6340879.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OO372_001337"
     CDS             complement(13786..14337)
                     /gene="smrB"
                     /locus_tag="OO372_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6340880.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            14503..15438
                     /gene="prmB"
                     /locus_tag="OO372_001338"
     CDS             14503..15438
                     /gene="prmB"
                     /locus_tag="OO372_001338"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6340881.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIIKIK"
     gene            15469..16554
                     /gene="aroC"
                     /locus_tag="OO372_001339"
     CDS             15469..16554
                     /gene="aroC"
                     /locus_tag="OO372_001339"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6340882.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            16558..17382
                     /gene="mepA"
                     /locus_tag="OO372_001340"
     CDS             16558..17382
                     /gene="mepA"
                     /locus_tag="OO372_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6340883.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            17382..18191
                     /locus_tag="OO372_001341"
     CDS             17382..18191
                     /locus_tag="OO372_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6340884.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            18191..18739
                     /gene="epmC"
                     /locus_tag="OO372_001342"
     CDS             18191..18739
                     /gene="epmC"
                     /locus_tag="OO372_001342"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6340885.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            18773..19051
                     /locus_tag="OO372_001343"
     CDS             18773..19051
                     /locus_tag="OO372_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6340886.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(19172..21178)
                     /gene="mnmC"
                     /locus_tag="OO372_001344"
     CDS             complement(19172..21178)
                     /gene="mnmC"
                     /locus_tag="OO372_001344"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6340887.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            21337..22557
                     /gene="fabB"
                     /locus_tag="OO372_001345"
     CDS             21337..22557
                     /gene="fabB"
                     /locus_tag="OO372_001345"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6340888.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            22832..24010
                     /locus_tag="OO372_001346"
     CDS             22832..24010
                     /locus_tag="OO372_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6340889.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(24007..25002)
                     /gene="flk"
                     /locus_tag="OO372_001347"
     CDS             complement(24007..25002)
                     /gene="flk"
                     /locus_tag="OO372_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6340890.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            25101..26237
                     /gene="pdxB"
                     /locus_tag="OO372_001348"
     CDS             25101..26237
                     /gene="pdxB"
                     /locus_tag="OO372_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6340891.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            26303..27316
                     /locus_tag="OO372_001349"
     CDS             26303..27316
                     /locus_tag="OO372_001349"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6340892.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            27316..28128
                     /gene="truA"
                     /locus_tag="OO372_001350"
     CDS             27316..28128
                     /gene="truA"
                     /locus_tag="OO372_001350"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6340893.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            28211..28870
                     /locus_tag="OO372_001351"
     CDS             28211..28870
                     /locus_tag="OO372_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6340894.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            29026..29940
                     /gene="accD"
                     /locus_tag="OO372_001352"
     CDS             29026..29940
                     /gene="accD"
                     /locus_tag="OO372_001352"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6340895.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            30010..31278
                     /gene="folC"
                     /locus_tag="OO372_001353"
     CDS             30010..31278
                     /gene="folC"
                     /locus_tag="OO372_001353"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6340896.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            31268..31930
                     /gene="dedD"
                     /locus_tag="OO372_001354"
     CDS             31268..31930
                     /gene="dedD"
                     /locus_tag="OO372_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6340897.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            32189..32677
                     /gene="cvpA"
                     /locus_tag="OO372_001355"
     CDS             32189..32677
                     /gene="cvpA"
                     /locus_tag="OO372_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6340898.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            32714..34231
                     /gene="purF"
                     /locus_tag="OO372_001356"
     CDS             32714..34231
                     /gene="purF"
                     /locus_tag="OO372_001356"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6340899.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            34326..34895
                     /gene="ubiX"
                     /locus_tag="OO372_001357"
     CDS             34326..34895
                     /gene="ubiX"
                     /locus_tag="OO372_001357"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6340900.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            35161..35943
                     /gene="argT"
                     /locus_tag="OO372_001358"
     CDS             35161..35943
                     /gene="argT"
                     /locus_tag="OO372_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6340901.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            36164..36946
                     /gene="hisJ"
                     /locus_tag="OO372_001359"
     CDS             36164..36946
                     /gene="hisJ"
                     /locus_tag="OO372_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6340902.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            37036..37722
                     /gene="hisQ"
                     /locus_tag="OO372_001360"
     CDS             37036..37722
                     /gene="hisQ"
                     /locus_tag="OO372_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6340903.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            37719..38435
                     /locus_tag="OO372_001361"
     CDS             37719..38435
                     /locus_tag="OO372_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6340904.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            38443..39216
                     /gene="hisP"
                     /locus_tag="OO372_001362"
     CDS             38443..39216
                     /gene="hisP"
                     /locus_tag="OO372_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6340905.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         9350 a         9672 c        10612 g         9843 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttagtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgtcgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgctgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgctgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatagttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgtgcgac ttaatgcccg taccgcctgc ggtagcagaa
     2941 tattctactg gcttgcggat gggcgacacc gccgaacaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgctc
     3361 ggtccagcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacgat gagcgaactg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgctcg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgcgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgatgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat tggcaactgc aaaacggcgc
     3961 aagaagcgga agcactggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg cggtgggctg gagctggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagtacagc attagagatg attctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagcc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggctatgac gctgaagctc gcgcctttgg cgaactggcg atgacgccgc
     4561 aatcgcaagc tttgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattaaaaca acagatggtg gcggaagtgg agcaaaattg cgccgctcat accatttttg
     4981 cttcgaacac gtcatcttta ccgattggtg atatcgccgc tcacgccgcg cgacctgagc
     5041 aagttattgg cctgcatttc ttcagtcctg tggaaaaaat gccgctggta gagattattc
     5101 ctcatgcggg tacatcggcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccgaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gcccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggcgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgaccttca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaacggacg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag cccttgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggcac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgagttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaatctca ctactatcga agactctccc catctataaa
     6661 taaaacactt tctactttca ttcggaaatt attcttctgc gcgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagaa aatagagttg aataatcagg caggatgtaa ttgaaacttg attaaattct
     6841 ggctatagtg tttatatatt atattgtcaa aagtggataa atgccaatcg cattatatgt
     6901 tcattgtttg ttttttaagc tgttgatatt cattattgat atactttcta ttttttctaa
     6961 atacttattc cgtatttttt aaggatgtcc ttaagataac ctttcttctg tcaatatatt
     7021 aattaaatct acatttactc tttagtaaaa ttaaattgca tcaggggtgt ggaaacatca
     7081 ctatgcggcc ccacaccaac gaatacaatt ttctacgacc tgttcttttt ggatcgcatg
     7141 ccgtattcaa tggctgggta taaacattgg ttattgactg aatcaatgta ttgcaatgtt
     7201 tatattttta attatttata gtttaaggac aaataatgag taagtttgtt aaaacagcta
     7261 ttgctgcggc aatggtgatg ggcgtgttca cctctacggc aacaatcgct gcgggtaaca
     7321 acggtactgc acgtttttac ggcaccattg aagactcagt gtgttctatc gttccggacg
     7381 atcacaaact ggaagttgat atgggtgata tcggtgccga aaaactgaaa aataacggca
     7441 ccaccacgcc gaaaagtttc cagattcgtc tgcaagattg cgtatttgat actcaggaaa
     7501 caatgaccac tacctttact ggtaccgttt cttctgcaaa tagcggcaat tattacacca
     7561 ttttcaatac cgatacctgt gcggcattta acaatgttag cctggcgatc ggtgactctc
     7621 tgggtacctc ttacaaaagc ggcatgggta ttgaccagaa aatagtgaaa gacacttcta
     7681 ccaataaagg caaagcgaag cagacactga actttaaagc ctggctggtc ggcgcagctg
     7741 atgcgccaga tctgggtaat ttcgaagcaa ataccacctt ccagattact tacctgtaag
     7801 gcataccccc atagcgacac tgctatgggg gatttaaatg gattttgacc gaacaagaat
     7861 aaaaacggat agaaacgtgt atgcctgacc attctctttt tcgattacgg attcttccgt
     7921 ggtgcattgc gctggcaatg tcagggagtt atagcagtgt ctgggctgaa gacgacattc
     7981 agtttgattc ccgttttctg gaattaaaag gcgacacaaa aattgatctg aagcgttttt
     8041 ccagccaggg atatgttgag cccggaaaat acaatttaca ggttcaacta aataaacagc
     8101 cattggcgga agagtacgat atttactggt atgctggtga agatgacgcg agcaaaagct
     8161 atgcttgtct gacaccggaa ctggtagcgc agtttggttt aaaagaagac gtggcgaaaa
     8221 atctgcaatg gagccacgat gctaaatgcc tgaaatccgg tcaactggaa ggcatggaaa
     8281 ttaaggctga tttaagccag tccgcattag tcatttcact gccacaggct tacctcgaat
     8341 atacttatcc cgactgggat ccgccttcac gttgggatga cggcatctcc gggatcgtcg
     8401 cggactacag catcaacgca caaacccggc acgaagaaaa tggcggtgat gatagtaacg
     8461 agatcagcgg caacgggacg gtcggggtta acctggggcc gtggcgtatg cgtgctgact
     8521 ggcagactaa ctatcaacat actcgcagta atgatgacga tgaattcagc ggcgatgaaa
     8581 ctcaaaaaaa atgggagtgg agtcgctact atgcctggcg ggcgttacca tcattaaaag
     8641 ccaaactggc gctgggcgag gattacctca gatccgatat ttttgatggt tttaactatg
     8701 ttggtggcag tgtcagtact gacgatcaaa tgttgcctcc caatctgcgc ggctacgcgc
     8761 cagacatttc cggcgtggca cacaccacag caaaagtgac cgtcagccag atggggcgtg
     8821 tgatttacga aacgcaggtt ccggctggac cgtttcgtat tcaggatctt ggtgattctg
     8881 tctccggtac gttgcatatt cgcattgaag aacagaacgg ccaggtgcag gaatatgaca
     8941 tcagcaccgc ctcgatgcca tacctcactc gtccaggtca ggttcgctat aagatcatga
     9001 tgggccgtcc gcaagagtgg ggacaccatg tcgagggtga atttttttct ggtgctgaag
     9061 cttcctgggg gatcgctaac ggctggtcgt tatatggcgg cgcactggga gatgaaaact
     9121 atcagtctgc ggcgcttggc gtcggtcgcg atttgtctac attcggcgcg gtcgcgtttg
     9181 atgttactca ctcgcacacc aaactggata aagacaccgc ttatggcaaa ggttcgctgg
     9241 acggtaactc cttccgtgtg agttattcca aagactttga ccagctcaac agccgcgtta
     9301 cttttgctgg atatcgcttc tcggaagaga actttatgac catgagcgag tatctggatg
     9361 ccagtgacag cggaatggta cgcacgggca acgacaaaga gatgtacact gccacttata
     9421 accagaactt ccgcgatgcg ggtgtttcgg tttatctcaa ctatacccgc catacctact
     9481 gggatcgcga ggagcagaca aactacaaca tcatgctctc gcactatttc aatataggca
     9541 gtattcgtaa tgtcagcatc tcgatgactg gctaccgtta cgagtatgac aaccaggccg
     9601 acaaaggcat gtacatttcg ctcagtatgc cgtggggtga caacagtacc gttagctata
     9661 acggtaacta tggcagtggg acggacagca gtcaggtcgg ttatttcagc cgtgtcgatg
     9721 acgcgactca ctatcagttg aacgtcggca ccagtgacaa acacaccagc gttgacggct
     9781 attacagcca tgatggttcg ctggcgcagg ttgacctcag tgcgaactac catgaagggc
     9841 aatacacctc tgcgggcttg tcgttacagg gcggcgcaac gcttactgcc cacggtggcg
     9901 cacttcaccg tacccagaat atgggcggga cacgcttgtt gattgatgcc gatggcgttg
     9961 ccgatgttcc ggtagaaggt aacggtgctg ctgtttatac caatatgttt ggtaaagccg
    10021 tcgtttctga cgtcaataac tattaccgca atcaggcgta tatcgacctc aacaaactgc
    10081 cggaaaacgc cgaagcaacc cagtcggtgg tacaagccac gctaactgaa ggggcgattg
    10141 gctaccgcaa atttgccgtc atcagtggtc aaaaagcgat ggcggtgctg cgtttacaag
    10201 acggcagcca tcctccgttt ggcgcagaag taaaaaatga taacgagcag acagtgggcc
    10261 ttgtcgatga tgacggcaat gtttatctgg ctggggtgaa acctggcgaa cacatgagtg
    10321 tgttctggag tggtgttgcg cattgcgata tcaacctgcc ggacccgctg cctgccgatc
    10381 tgtttaacgg cttgttactg ccatgccagc ataaaggcaa cgtcgcgccg gttgttcctg
    10441 atgacataaa gcctgtcatt caggagcaga cgcaacaggt gacacccacc aatccccctg
    10501 tttctgtttc agctaaccaa taacgtgatt aaggaatgat ccatgtctga tttactttgc
    10561 tctgccaaat tgggtgctac gacgctagcg ttactgctta gcgctgccag tttgtcgccc
    10621 aggcttctgt aacgccagat cgtacccgtt tgatatttaa tgaaagcgac aaatcaatca
    10681 gcgtgacgtt gcgcaataac gatccgaaac tgccttatct ggcccaaagc tggatcgaag
    10741 atgaaaaggg taacaagatc agttcaccgt taactgttct gccaccggtg caacgcatcg
    10801 attcaatgat gaacgggcag gtaaaagtgc agggaatgcc ggatatcaac aaattgcctg
    10861 ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat cccgccaaaa tcaaacaaag
    10921 ccaacacgct gcaaatagca ctacaaacgc gtattaagtt gttctggcga ccgaaggcgc
    10981 tggagaacgt cagcatgaaa aacccctggc aatataaggt tactttaacc cgcaacggtc
    11041 aggaatttac cgtcaataac ccgacgccgt attacgtcat tttcagtaat gccagcacac
    11101 agaaaaatgg caatcctgca gccggtttct cgccaatagt gatgtcgccg aaaacctctg
    11161 caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac ctatgtcaac gactatggcg
    11221 ctcggatgcc gctctttttc acttgcaacg gtaacagttg ccaggtcgat gaagagcaga
    11281 gccgcaaagg ctgaggccgg gagagccagt atgaaaggat cttgttctgt ctgggcggta
    11341 actgggttac tgatatgttg tgttggtagc ggctttgcag ttgcagcgag taagtcagta
    11401 agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt gtacggtaaa tggtgcggca
    11461 gttgagtttg gtaatgtatt cattaataag atcaatgggg ttgattataa acgtcccatt
    11521 gactactcgc ttgtttgtaa caatctggca atggatgacc tgcgtctaca gatgcaggcg
    11581 acaacggtcg tgattaacgg tgaaacagtt atcagcacgg gtattcctgg gtttggtatt
    11641 cgggttcaga aaagtagtga tcacacaata ctcgacttaa cctctggctc atggttgcca
    11701 tttaattttt cctcgggagt tcccgttctg gaagctgtgc cggtgaaaca gagtggtaca
    11761 acgttagcgg cagctgaatt taacgccagt gccacaattg tggtggatta ccaatgaaac
    11821 ggatctcgct tattttgctg tggggctttt gctcgatggc actctcaaac gtctcttttc
    11881 acggttattt agtgcagccg cctaactgca cgattagcaa cgctcaaacc attgaaataa
    11941 cttttcagga tgtgcttatt gatgacatta atggcagcaa ttatgagcaa accgtgccgt
    12001 attccattac ctgcgatacg gcggttcgcg acccattaat ggaaatgacc ttgagctgga
    12061 gtggcacact aagcgatttt gataatgctg cagtaagcag caatatcact ggtttaggca
    12121 ttcagctcaa acaggcaggg cagagtttta cgatcaatac gccacttatg gtgaatgaaa
    12181 ccgatttacc ggtgttaacg gctgtgccag taaaaaagag tggtgtcgtt ctgccagagg
    12241 cggactttga ggcctgggcc acgttacagg tagattacca atgaagaaaa agcgtacgtt
    12301 gttttttatt agtagcctga tgttgctggg cagtggaacc acaatagcag gagataattt
    12361 gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca gtgattaatg gtagccagtt
    12421 ggcagaagta cattttcctg ccattgccgc cagcgattta atgaaccttg ggcagtcaga
    12481 gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt tctacattgt ttaatgtcaa
    12541 agtaacgttg acaggaacag aagatagtgc tctacccggc tttctggcct ttgactcctc
    12601 gtcatcagca tccggcgctg ggattggtat tgaaactgcg gcaggcacat cggtgccaat
    12661 caacaataca acaggcgtca cgctcccgct gaatcaggga aataacagcc taaattttaa
    12721 tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc ggagacttta gtgccacagt
    12781 gacggcaacc tttgagtatt tctgaaatga aaaaatggac cataattttc gcgagcctaa
    12841 tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta tgctggagat aaacttatga
    12901 gtgcgagttt tgacagtgtg agagtttatt atgccatgga caaggtaacc ggtgctgtag
    12961 cgagcagtgt tttcaatgtt acggtgataa cgccaaagga ggttacatat gggaaatatg
    13021 attcttttgc gtataaaggc gatacgttgc gggttatttc ctggtctggg tcgggttcag
    13081 ctccaacttt ggtactgact gactttgata caattaataa ctcaaattgc cctggcatag
    13141 atacgaaaat ttttcgttgt gcatatatga cgtttaaaat taccgttgca tctgatgatt
    13201 atggttgccc ttggatcgca tccttttatt cctatacaga tttacccgga tttggaagct
    13261 atacggcccc tacagtgcat aacacaatat gcccaacgat accggtcact tcatacgata
    13321 tctcctggag tgaaaattat gtcagccata ataaggcgtt acgaattcag tccacggggt
    13381 caacagtgac caccacgctt tcgacctacc tgatggaagg cggcagactc tgtgatggca
    13441 gtaatttttc tgataatgat ggcagaggag catactgccg cgcggtatca gaattgttaa
    13501 cctttacctc ttacggttgt gataaatcaa cggtaacggt tacgccaact cgtcatccgg
    13561 taacggataa agtactacac gatattgtgg tgaatgtgaa taccagtagt gggcaaccga
    13621 ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact gtagttcgta gtattgctct
    13681 gatgcgtctt atcaggccta cgaagtgaat tgcaatttat tgaattatca catatttgta
    13741 ggccggataa ggcgtttacg ctgctccggc atttgctgcc agcattcagg gcaactccgg
    13801 cggcaaccac tcttccactt caatcaacac caacaaagcc gcatcaccgc cgtactcttt
    13861 cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc agccacagcg gtgtttgttg
    13921 cttcaaaata tgcttcccgt gcccatgcat cacgcaggca caaaacacat gttcgcggcg
    13981 acaggcggca atcaacgcgc ccagttcctg cttggcctgc agctgcgtca gaccgtgtaa
    14041 atccaaaaac aattccggtg aataatcgcc gcggcgcagt ttcttcgcct caaaatggct
    14101 gacatccggg cgaacatatt tcaccggacc ttcggtattt aataacggct gaaactcatc
    14161 ggagaaataa tggctggcat cagcctgctc cgggatcaag cgtttcaccg gcacttcgct
    14221 gattttttta cgctgcggtc ggtggacaat cgtgtcctgc ttaatcttgc gagtccccgc
    14281 catcaactgg cgaaacagag cctggtcctc ctcgctaagt gttgttttct ttttcattta
    14341 tctgtctcta tttctaattt tcctacagtt tacccgactc gtagcacaat cgatcgtttt
    14401 aaacgtattt ttcctcactc accgcgcgtg aatgctgatt tatcgtcatc ttcatggcaa
    14461 actagccgcc gaaattattg cgatcatgcc ctggaggaat acgtggataa aattttcgtt
    14521 gatgaagcag taaatgagct gcaaaccatt caggacatgt tgcgctggtc ggtaagccgc
    14581 ttcagcgcgg caaatatctg gtacggtcac ggcaccgata acccgtggga tgaagccgta
    14641 cagctggtgt taccatcgct ctacctgccg ctggatatcc cggaagatat gcgcaccgcg
    14701 cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg tgatccgccg cgtcaacgag
    14761 cgcattccgg tggcttacct gaccaacaaa gcctggttct gcggtcatga attttacgtc
    14821 gatgaacgcg tgctggtgcc gcgctcgccg attggtgaac tgatcaacaa taaatttgcc
    14881 ggactcatta gcaagcaacc gcagcatatt ttagatatgt gtaccggtag cggctgcatc
    14941 gccattgcct gtgcttatgc cttcccggaa gcggaagtcg acgcggtaga tatctctccc
    15001 gacgcgctgg cggttgccga acagaacatc gaagaacacg gtctgatcca caacgtcatt
    15061 ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc agtacgatct gattgtcact
    15121 aacccgccgt atgtcgacgc ggaagatatg tccgacctgc caaacgaata ccgtcacgag
    15181 ccggaactgg gcctggcatc tggcaccgac ggccttaaac tgacgcgtcg cattcttgga
    15241 aacgcggcag attaccttgc tgatgatggc gtgttgattt gtgaagtcgg caacagcatg
    15301 gtacatctta tggaacaata tccggatgtt ccgttcacct ggctggagtt tgataacggc
    15361 ggcgatggtg tgtttatgct caccaaagag cagctgctgg cggcccgtga atatttcgcg
    15421 attataaaga ttaagtaaac acgcaaacac aacaataacg gagccgtgat ggctggaaac
    15481 acaattggac aactctttcg cgtaaccacc ttcggcgaat cgcatgggct ggcgctcggc
    15541 tgcatcgtcg atggtgttcc gccaggcatt ccgctgacgg aagcggacct gcaacatgac
    15601 ctcgaccgtc gtcgccctgg gacatcgcgc tataccaccc agcgccgcga gccggatcag
    15661 gtcaaaattc tctccggtgt ttttgaaggc gttactactg gcaccagcat tggcttgttg
    15721 atcgaaaaca ctgaccagcg ctctcaggat tacagcgcaa ttaaagacgt tttccgccca
    15781 ggccatgctg attacaccta cgaacaaaaa tacggtctgc gcgattatcg cggcggtgga
    15841 cgttcttccg cccgcgaaac cgccatgcgc gtggcggcag gggcgattgc caaaaaatat
    15901 ctcgccgaga aatttggtat tgaaatccgc ggctgcttga cccagatggg cgacattccg
    15961 ctggaaatca aagactggtc gctggttgaa caaaatccgt tcttctgccc ggacccggac
    16021 aaaatcgacg cgttagatga actgatgcgc gcgctgaaaa aagagggcga ctccatcggc
    16081 gcgaaagtca ccgttgttgc cagtggtgtc cccgccggac ttggcgagcc ggtctttgac
    16141 cgcctggatg ccgacatcgc ccatgcgctg atgagcatca acgcggtgaa aggcgtggaa
    16201 attggcgacg gttttgacgt agtggcgctg cgcggcagcc agaaccgcga cgaaatcacc
    16261 aaagacggtt tccagagcaa ccatgcgggc ggcattctcg gcggtatcag cagcgggcag
    16321 caaatcattg ctcatatggc gctgaaaccg acctccagca ttaccgtgcc ggggcgcact
    16381 attaaccgct ttggcgaaga agttgagatg atcaccaaag gccgtcacga tccctgtgtc
    16441 gggatccgtg cagtgccgat cgctgaagcg atgctggcga tcgttttaat ggatcacctg
    16501 ttacggcagc gggcgcaaaa tgccgatgtg aagactgata ttccacgctg gtaaaaaatg
    16561 aacaaaaccg cgattgcgct gctggctctg cttgccagta gcgctagcct ggcagcgacg
    16621 ccgtggcaaa aaataaccca acctgtgccg ggtagcgcac aatcgatagg cagtttttct
    16681 aatggctgta ttgtcggcgc tgacacgttg ccgatacagt ccgaacatta tcaggtcatg
    16741 cgtaccgatc agcgtcgcta tttcggtcac ccggatctgg tgatgtttat ccagcgtctg
    16801 agtagccagg tgagcaatct gggcatgggt acggtgctga ttggcgatat ggggatgccc
    16861 gctggtgggc gtttcaacgg cggtcatgcc agccaccaga ccggactgga tgtcgatatc
    16921 tttctgcaac tgccgaaaac tcgctggacc tccgcgcagc tcttgcgccc gcaagcactg
    16981 gacttagttt cccgcgacgg taaacacgtt gtctccacgc tgtggaagcc agaaattttc
    17041 agcttgatca aactcgctgc ccaggacaaa gacgtcacgc gcatttttgt taatccggcg
    17101 attaaacaac aactttgcct tgatgcgggc accgatcgcg actggttgcg caaagtgcga
    17161 ccctggttcc agcatcgcgc gcatatgcat gtacgattac gttgccctgc cgatagtctg
    17221 gagtgtgaag atcaaccttt accgccatca ggcgatggtt gcggggcaga actgcaaagc
    17281 tggtttgcac ctctaaaacc gggaacaaca aagcctgaga agaagacacc gcctccgttg
    17341 ccgccttcct gccaggcgct actggatgag cacgtgatct aatggaaacg tttaatagcc
    17401 tgtttatggt ttccccgctg ttgctgggag ttctcttttt tgtcgccatg ctggcgggat
    17461 ttatcgactc gattgccggt ggcggtgggt tactcaccat tccggcattg atggctgcgg
    17521 gaatgtctcc cgctaacgcg ctggcaacca ataaactgca agcctgcggc ggctctattt
    17581 ccgccactat ctactttatt cgccgcaaag tggttagttt aagcgatcag aaactcaata
    17641 ttgccatgac ctttgtcggc tcaatgagcg gcgcactgtt ggtacagtac gttcaggctg
    17701 atgtcttacg gcagattttg cccattctgg tgatttgtat cggcctctat ttcttgctga
    17761 tgccaaagct gggtgaagaa gatcgccagc gccggatgta cggtttaccc ttcgcgttaa
    17821 ttgccggtgg ctgcgtcggt ttttatgacg gattctttgg cccggctgcc ggatcgtttt
    17881 acgcgctggc cttcgttacg ctgtgcggat tcaacctcgc caaagccacg gctcacgcca
    17941 aattactcaa cgcaacgtca aacattggcg gtctgctgct gtttattctc ggcggcaaag
    18001 tgatttgggc gacgggcttt gtcatgctgg tggggcagtt cctcggcgcg cgcatgggtt
    18061 cacggctggt gttgagcaaa ggacaaaagc tgatccgccc gatgattgtt atcgtctcgg
    18121 cggtgatgag tgccaaatta ctttacgata gccacggaca ggagatcctc cactggttgg
    18181 ggatgaacta atgaacagta cacaccacta tgagcagttg attgaaattt ttaatagctg
    18241 ctttgccgat gaatttaata cccgtctgat taaaggcgac gacgaaccga tctatcttcc
    18301 tgctgatgcg gaagtaccgt ataaccgaat tgtctttgcc cacggctttt atgccagcgc
    18361 aattcatgag atttcgcact ggtgtattgc cggaaaagcg cgccgtgagc tggttgattt
    18421 cggctactgg tattgcccgg atgggcgtga tgcccaaacg caaagccagt ttgaagatgt
    18481 tgaagtgaag ccgcaggcac tcgactggtt gttctgtgtg gcggcgggat atccgtttaa
    18541 cgtcagttgc gacaatctgg aaggcgactt cgaaccggat cgtgttgtgt ttcaacggcg
    18601 ggtacatgcg caggtgatgg attatctgac aaatggcatt cccgagcgtc cggcacgctt
    18661 cattaaagca ctacaaaatt attatcacac gccggaactt acggcggaac agttcccgtg
    18721 gccggaagcg ctcaactgaa gccggtgcca cgttttacaa tgaggaaaga agatgatcgc
    18781 ggagtttgaa tcacgcattc tggcattaat cgacggcatg gttgaccatg ccagtgatga
    18841 tgagttgttt gccagtgggt atttgcgtgg ccacctgaca ttagccatcg cagaactgga
    18901 aagtggtgat gaccactccg ctcaggcggt gcatacgacc gttagccaga gtctggaaaa
    18961 agccattggt gccggtgaat tgtcgccgcg tgaccaggcg ctggtgaccg atatgtggga
    19021 aaacctgttt cagcaggcgt cacagcagta agttgcggcg tctggagcac catgcctgat
    19081 gcgacgctta tcgcgtctta tcaggcctac acgtcacacg taggtcggat aaggcgttca
    19141 cgccgcatcc ggcaaacact gcgagagcag attaccccgc cttaaccgct ttacccttca
    19201 acaatttccg cacccataac cgattcgggt ttaacgccgt cagcgtactg gcatccatcg
    19261 gaatcggttc gtcgctcatc tgcgccgcca gaatctcggc acacagcggg gcagaacaca
    19321 aaccgcgaga acctaaagcc gcaaacataa agagatcgtc aaaaaccggc gcgcttaccg
    19381 cctcatcttt ctgctccgcc aacgacgcat attccacgag tgttgcctca taatcgggaa
    19441 cattgcctac cattggcaga tgatcgcggg tggcacaacg cacaccgcag cgcgcctctt
    19501 tatcactgac atcaacctct tttgcccact gtgcctgcgg gaaacaatca atcaaccgct
    19561 ggcgattctg ctgctgatcg tcctcactgt acgccgtatc ttcgctgccg cgatgataac
    19621 tggcaccaat acaatgatgt tgattcgccg gattttgtgg cgtgagataa ccgtcatagc
    19681 acagcacctg cttcagctct gccagttccg gcgttgtcgg aatatggctg acctgcccgg
    19741 caaccgaata caccgggaga gtcgacgttt ggctgaaacg gctgatttga tgcccgttcg
    19801 ccagtaccac tacgctgtgt gttgcttgct gatctcctgc aaaattcaac aaccaacagt
    19861 catccttacg ggataaattc tgtaactgat attgataata aatctgcaaa ccctgctgtt
    19921 gcgccagttc cagcacatta cgggtcagtt ctgctgggca cagccaacca ccttgcggat
    19981 aagtaatgcc gctgcaattt gtcgcaacgc ccgtaatttg ttcaaccgca tttgcctcaa
    20041 cggctacagc cagttctgcg ggtaaatcca ttgacaacat ctgtgcgatt ttatgctggc
    20101 ttttctcatc ccagcctaac tgcgtgacgc cgcaccagtc atgatcaaat ttaacgggta
    20161 attggtcgta aaaccgacga gcaaaagtaa acgcattaga gaaaaagcgg tttagcgcct
    20221 catcgtgttt gcttaataac ggatacagcg ccccctggcg attgccggaa gcacccagcg
    20281 cgggggcttc atccgcgcaa taaagcgcta cctgccagcc gcgccgtaat aacgccagcg
    20341 acaacaacgc gctggcaata ccaccgccga taatcgctac ttcccgtttg ctgctgcccg
    20401 tacgattaaa ccacggcgtg gagcagggga gtggtaatgt ctgttccatc accccacaaa
    20461 gcatttcccg tttgcgccca aagcccttac gtttttgcat cgtgaatccg gcttcctgca
    20521 aaccgcggcg gacaaaaccg gcagacgtaa atgtcgccag cgtgccgcct ggacgcgcca
    20581 accttgccat ggcgttaaac agattttgcg tccacatatc cgggttttta gctggcgcaa
    20641 agccgtccag aaaccaggca tctacttttt gatttagcga atcgtccagt tggctggtca
    20701 gttcgttaat atcgccaaac cataaatcca gcgtcacgcg gccttcattg agcaataaac
    20761 gatggcaacc gggcaagggc attggccact gcgcctgaag ttgttctgcc cacggagcca
    20821 gttccggcca gtgttgatgc gctaaggcta aatccgcacg ggtgagggga aatttctcaa
    20881 aactaatgaa atgtaagcgt tgtaattgcg cttgcggatg cgcttcgcga aactgatcaa
    20941 atgcctgcca tagcgtcagg aagtttaatc cggtgccgaa gccgctctct gctaccacaa
    21001 acagaggatg tggatgctca ggaaagcgta cctctaattg gttgcctccc agaaaaacat
    21061 aacgcgtctc ttccagcccg ttatcgttgg aaaaatagac atcgtcaaaa tctcgggaaa
    21121 caggtgtacc ctcagcatta aattcgaggt tggcaggttg tatggagtag tgtttcacgt
    21181 aagttactcg tcttacaggc ggtggctcga tcttagcgat gtgtgtaagg ctgcgcaaat
    21241 ttctctatta aatggctgat cggacttgtt cggcgtacaa gtgtacgcta ttgtgcattc
    21301 gaaacttact ctatgtgcga cttacagagg tattgaatga aacgtgcagt gattactggc
    21361 ctgggcattg tttccagcat cggtaataac cagcaggaag tcctggcatc tctgcgtgaa
    21421 ggacgttcag ggatcacttt ctctcaggag ctgaaggatt ccggcatgcg tagccacgtc
    21481 tggggcaacg taaaactgga taccactggc ctcattgacc gcaaagttgt gcgctttatg
    21541 agcgacgcat ccatttatgc attcctttct atggagcagg caatcgcaga tgcgggcctt
    21601 tctccggaag cttaccagaa taacccgcgc gttggcctga ttgcaggttc tggcggcggt
    21661 tccccgcgtt tccaggtatt cggcgctgac gcaatgcgcg gcccgcgcgg cctgaaagcg
    21721 gttggcccgt atgtggtcac caaagcgatg gcatccggcg tttctgcctg cctcgccacg
    21781 ccgtttaaaa ttcatggcgt taactactcc atcagctccg cgtgtgcgac ttccgcacac
    21841 tgtatcggta acgcagtaga gcagatccaa ctgggcaaac aggacatcgt gtttgctggc
    21901 ggcggcgaag agctgtgctg ggaaatggcc tgcgaattcg acgcgatggg tgcgctgtct
    21961 actaaataca acgacacccc ggaaaaagcc tcccgtactt acgacgctca ccgtgacggt
    22021 ttcgttatcg ctggcggcgg cggtatggta gtggttgaag agctggaaca cgcgctggcg
    22081 cgtggtgctc acatctatgc tgaaatcgtt ggctacggcg caacctctga tggtgcagac
    22141 atggttgctc cgtctggcga aggcgcagta cgttgtatgc agatggcgat gcatggcgtt
    22201 gataccccaa tcgattacct gaactctcac ggtacttcga ctccggttgg cgacgtgaaa
    22261 gagctggcag ctatccgtga agtgttcggc gataagagcc cggcgatttc tgcaaccaaa
    22321 gccatgaccg gtcactcact gggcgctgcg ggtgtgcagg aagctatcta ctctctgctg
    22381 atgctggaac acggctttat cgccccgagc atcaacattg aagagctgga cgagcaggct
    22441 gcgggtctga acatcgtgac cgaaacgacc gatcgcgaac tgaccactgt tatgtctaac
    22501 agcttcggct tcggcggcac caacgccacg ctggtaatgc gcaagctgaa agattaattc
    22561 gcagtaggcc ggagtaggtc ggataagacg cgccagcgtc gcatccgacg ttacgcgcca
    22621 atgcggcctc cggcactaac gcaaaaggga acctgatggt tccctttttc acatcattga
    22681 caatcgccac cagttccagg caaacttccc gctttgtcga tttccttctg aaaagacgta
    22741 cgcgttaaat cctgccaacg caccgtaacc ctgaaaccag agagatgaga cggggatact
    22801 cctcgccttg cgctgcattc tggagtaatg catgactgct gtaagccaaa ccgaaacacg
    22861 atcttctgcc aatttttcgc tcttccgcat cgcttttgcg gtttttctca cctacatgac
    22921 cgtagggctg ccgttgccgg ttatcccgct gtttgttcat catgaactgg gctatggcaa
    22981 taccatggtc ggcattgccg tcgggattca gtttctggct acggtgctga cgcgtggcta
    23041 tgccgggcga ctggccgatc aatatggcgc aaaacgttcg gcgcttcagg ggatgttagc
    23101 ttgtggtctg gctggcggcg cgttgctgct ggcggcgatt ttgcctgtct ccgcaccgtt
    23161 caaatttgcc ctgttggtca tcgggcgttt gattcttggg tttggtgaaa gccagttact
    23221 gacaggcgct ctgacctggg ggttaggcat cgtagggcca aaacactctg gcaaagtgat
    23281 gtcatggaat ggaatggcga tttacggtgc cctcgctgtt ggtgctccgc ttggcctgtt
    23341 gattcatagc cattacggtt ttgccgcact ggcgatcacc acaatggtat tacccttact
    23401 ggcgtgggcc tgtaacggca cagtgcgcaa agtaccggcc ctggcgggag aacgtccatc
    23461 gctgtggagc gttgtcgggt ttatctggaa accagggtta gggctggcac tacaaggcgt
    23521 tggttttgcg gttatcggga ctttcgtttc gctctacttt gccagcaaag gatgggcgat
    23581 ggcgggcttt actcttaccg agtttggcgg cgcatttgtc gtgatgcgcg tcatgtttgg
    23641 ctggatgccg gaccgttttg gcggcgtgaa agtggcgatt gtctctctgc ttgtagaaac
    23701 ggtgggcttg ttgctgctct ggcaagcccc aggtgcgtgg gtcgcattag cgggcgcggc
    23761 gttaaccgga gccggatgtt cgcttatctt tcctgcgctg ggcgtggagg tggttaaacg
    23821 cgtcccctca caagttcgcg gcaccgcact gggcggttac gccgcgtttc aggatatcgc
    23881 cctcggcgtc tccgggccgc tggcgggaat gctggcgacc acgtttggtt actcttcggt
    23941 atttcttgcc ggggcgatct ctgcggtgct gggaattatt gtcacgatac tgtcatttcg
    24001 tcggggctaa cgaaccagcc agaccagcat caggataatc gcgactaaca gtatccacag
    24061 cgcgggacgc gttgccatat tttgcaacgt gtccatcatc ggtggaaaag gattaagcag
    24121 cggttgcata tcacgcggat caatcccttt aacccgccgt tgataaagct gattaagaat
    24181 atcctcggcc tgggcagaag aaagcgatga ttgcgcagaa agaccatatt tttgctggat
    24241 atacgccgat aacgctgcca gttcactggc atctaaaggt tgttttagcg ccatctgtag
    24301 tgattccagc gtcggcgtat tttgctggct taacgtctgg cgcgcctgca accaggtcac
    24361 cagatggtta aacagtttcg ctggaattaa ctcgccatcg ttcaccccgg aaagttccag
    24421 catcgattgc cagatctgtt tgctgggttc ccccgttgcc gccgcaagtt tggtcaccag
    24481 ctgttttagc gcattgtgct ccgccggtaa taaaggacgg tcggtcgcct cgcgctgctg
    24541 tggttgcgga ataaccatct tcccttcctg caacagggtg agaatggttt ttaattgctc
    24601 cggtgagagc tgattcagcg gcgtctgacc aaagttatga cggatataat ccgtgaccgc
    24661 ctgacgatta ttccccagac gtaaatactc ccctaattgc gctaaaagct ggcgggcaga
    24721 atggcttttt tgcgcggcca gcagacgttg cgccagatta tgctcagcgg cagggaagtg
    24781 acgcgaaagc agcggtgaat ctcccgacag accaatatcg tgcctgatac ccgcccacag
    24841 ttctgctctt tgttgttgcg tcagtgaggt cactttcgtc attaagcttt ccagcgaagt
    24901 acgttgctga ctggataaag gctgattgcc cgcgccagac ggcaggttat ctccctgacc
    24961 tggtggttgc ccaggaggag ggccggaaat aggttgtatc attacgtatc cttatacctg
    25021 aaatcttcgc aagtatgcct ggccgcgaga ttatggcaca cttgtccggt taactctcgt
    25081 ctcatacagg taacacaaac gtgaaaatcc ttgttgatga aaatatgcct tatgcccgcg
    25141 acttatttag ccgtttgggt gaggtgaccg cggttcccgg gcgtccaatc cccgtcgctc
    25201 aactggcaga cgcggatgcg ctgatggtgc gttcggtcac gaaagtgaat gaatctttgc
    25261 tggcaggaaa acccattaaa tttgttggca ctgccactgc ggggaccgac catgtcgatg
    25321 aagcatggtt gaagcaggcg ggaattggtt tttccgctgc acctggctgt aatgcgattg
    25381 cggtggtgga atatgttttc tcctcactac tgatgcttgc cgaacgcgat ggattttcac
    25441 tgcacgaccg tacggtgggg atcgtgggcg ttggtaacgt tggacgtcga ttgcaggcgc
    25501 gactggaagc gttagggatt aaaacattgc tttgcgatcc gcctcgcgct gaccgtgggg
    25561 atgagggtga tttccgctcg ctggatgaat tagttcagca tgcggatatt ctgactttcc
    25621 atacgccact cttcaaagat gggccgtaca aaacgctgca tctggcggat gaaaaactga
    25681 tccgtagcct gaagccggga gcgattctga ttaacgcctg ccgtggcgca gtcgtcgata
    25741 atactgcgtt gctgacctgc ctgaatgaag gccagaagtt aagcgtagtg ctggatgtct
    25801 gggaaggcga accggaactt aacgtggagt tgctgacaaa agtggatatc ggcacgccgc
    25861 atatcgcagg ttataccctg gaaggtaaag cacgcggtac tacgcaagtg tttgaagcct
    25921 atagcaagtt tattgggcat gaacagcacg ttgcgctgga tacattactg cccgcgccag
    25981 agtttggtcg cattacgctg catggcccgc tcgatcaacc gacgctgaaa aggctggtgc
    26041 atttggtgta tgatgtgcgc cgcgatgacg caccgctgcg taaagtcgcc gggataccgg
    26101 gtgagttcga taaactgcgc aaaaactatc ttgagcgccg tgaatggtca tctctgtatg
    26161 taatttgtga tgacgccagt gcggcatcat tgctgtgtaa actgggtttt aacgccgttc
    26221 atcatccggc acgttaatct cttcttcatg ctctctgctg taacattggc agggagcttt
    26281 gctatttctg gagtaaacca ccatgtctga aggctggaac attgccgtcc tgggcgcaac
    26341 tggcgctgtg ggcgaagccc tgcttgaaac gctggctgaa cgtcagttcc cggttgggga
    26401 aatttatgca ctggcacgta acgaaagcgc aggcgaacaa ctgcgctttg gtggtaaaac
    26461 aatcactgtg caggatgccg ctgaattcga ctggacgcag gcgcagctgg cattttttgt
    26521 cgcaggcaaa gaagctaccg ctgcctgggt tgaagaagcg accaactcag gttgcctggt
    26581 gatcgacagc agcggactgt ttgctctcga acccgacgta ccgctggtgg tgccggaagt
    26641 aaacccgttt gtactgaccg attaccgcaa ccggaatgtc atcgccgtac cagacagtct
    26701 gaccagccag ctgctggcgg cactgaaacc gttaatcgat cagggcggtt tgtcgcgtat
    26761 cagtgttacc agcctgattt cagcctccgc ccagggcaaa aaagcggtcg atgcgttagc
    26821 ggggcagagt gcgaaattgc tcaacggcat tccgattgac gaagaagatt tcttcggtcg
    26881 tcaactggcg ttcaatatgc tgccgttgtt gccggatagc gaaggtagcg tgcgtgaaga
    26941 acgtcgtatc gttgacgaag tacgcaaaat cctgcaggac gaagggctga tgatttcggc
    27001 aagcgtcgtc caggcaccgg tattctacgg tcatgcccag atggtcaact ttgaagcact
    27061 gcgtccgctg gcggcagaag aagcgcgtga tgcgtttgct cagggcgaag atattgtgct
    27121 ctctgaagag aacgaattcc cgactcaggt aggggatgct tcgggtacgc cgcatctttc
    27181 tgttggctgc gtgcgtaatg actacggtat gccggagcaa gtccagttct ggtcggtggc
    27241 cgataacgtt cgctttggcg gcgcgctgat ggcagtaaaa atcgccgaga aactggtgca
    27301 ggagtatctg tactaatgtc cgaccagcaa caactgccag tttataaaat tgcgctgggc
    27361 attgagtacg acggcagtaa gtattacggc tggcaacggc agaatgaagt ccgcagtgtg
    27421 caggagaagc tggaaaaggc gctctcccag gtggcgaacg aacccatcac cgtcttctgc
    27481 gccgggcgta ctgacgcagg ggtacacggt accgggcagg tggtgcattt cgaaacaacc
    27541 gcgcagcgca aagacgcggc gtggaccttg ggcgtaaatg cgaatttacc tggtgacatc
    27601 gccgtgcgtt gggttaaagc tgtacctgat gattttcatg cccgatttag cgccacggct
    27661 cgccgttatc gctacatcat ctacaatcat cggctgcgcc cggcggtatt gagtaaaggg
    27721 gtaacccatt tttacgaacc gctggacgct gaacggatgc atcgggctgc gcaatgcttg
    27781 ctgggcgaga atgatttcac ctcgttccgt gcggtgcagt gccagtcccg aaccccgtgg
    27841 cgcaacgtta tgcatattaa tgtcacgcgt cacggtcctt atgtggtggt agatatcaaa
    27901 gcgaatgcct ttgtacatca tatggtcagg aatattgtcg gcagcctgat ggaagtcggt
    27961 gcccacaacc agccggagag atggatagca gagttgctgg cggcaaagga cagaacgctg
    28021 gcggcagcaa cggcaaaagc ggaagggctg tatctggtcg cggtggatta ccctgaccgg
    28081 tatgatcttc caaaaccgcc aatgggcccg ctatttctgg cggactaacg agaataatgc
    28141 tcggaacatt tcaggctgta gccaacgtag ttacagcctg aaagatgacg agtacaaggc
    28201 ataggcaaat atggacctga tttatttcct catcgatttt atcctgcaca ttgacgtgca
    28261 tctggcagag ctggtcgcgg agtacggcgt ctgggtttat gccattctgt ttttgattct
    28321 gttctgcgaa acggggctgg tggtaacgcc attcctgcct ggtgattcgc tgctgtttgt
    28381 tgctggtgca ttggcgtcgc tggaaactaa cgatctcaat gtccatatga tggtggtgct
    28441 tatgttgatt gccgctattg tcggtgatgc agtgaactat accattggac ggttatttgg
    28501 tgacaaactg ttcagtaacc cggattcgaa aatctttcgt cgcagttatc ttgataagac
    28561 tcatcagttt tacgagagac atggcggcaa aacgattatt ctcgcccgtt ttgtaccgat
    28621 cgtcagaacg ttcgcgccgt ttgttgcggg aatgggccac atgtcgtacc gtcatttcgc
    28681 cgcttataac gtgatcggcg cactgttgtg ggtactgatt tttacctacg cgggttattt
    28741 cttcggcacg ctccccttca ttcagagcaa ccttaagctg atgattgttg gcattatttt
    28801 tgtttcaata ttgcctgggg ttttcgaatt tattcgtcac aaacgcgctg cggcacgcgc
    28861 cgcaaaataa aaagtaactc cgcggttcga ccactttttt atccaaagtt tcgggctgtt
    28921 atgttttaat gtgcaacatt catggtctgt tgggggcaaa aatggcatta tgcgtcccca
    28981 aagataaaac tggcatcgaa ccaggttcag acagaaaggt ccctaatgag ctggattgaa
    29041 cgaattaaaa gcaacattac tcccacccgc aaggcgagca ttcctgaagg ggtgtggact
    29101 aagtgtgata gctgcggtca ggttttatac cgcgctgagc tggaacgtaa tcttgaggtc
    29161 tgtccgaagt gtgaccatca catgcgtatg acagcgcgta atcgcctgca tagcctgtta
    29221 gatgaaggaa gccttgtgga gctgggtagc gagcttgagc cgaaagatgt gctgaagttt
    29281 cgtgactcca agaagtataa agaccgtctg gcttctgcgc agaaagaaac cggcgaaaaa
    29341 gatgcgctgg tggtgatgaa aggcactctg tatggaatgc cggttgtcgc tgcggcattc
    29401 gagttcgcct ttatgggcgg ttcaatgggg tctgttgtgg gtgcacgttt cgtgcgtgcc
    29461 gttgagcagg cgctggaaga taactgcccg ctgatctgct tctccgcctc tggtggcgca
    29521 cgtatgcagg aagcactgat gtcgctgatg cagatggcga aaacctctgc ggcactggca
    29581 aaaatgcagg agcgcggctt gccgtacatc tccgtgctga ccgacccgac gatgggcggt
    29641 gtttctgcaa gtttcgccat gctgggcgat ctcaacatcg ctgaaccgaa agcgttaatt
    29701 ggctttgccg gtccgcgtgt tatcgaacag accgttcgcg aaaaactgcc gccaggattc
    29761 cagcgcagtg aattcctgat cgagaaaggc gccatcgaca tgatcgtcca tcgtccggaa
    29821 atgcgcctga aactggcgag cattctggcg aagttgatga atctgccagc gccgaatcct
    29881 gaagcgccgc gtgaaggcgt agtggtaccc ccggtaccgg atcaggaacc tgaggcctga
    29941 taactgataa gggcagggcc actggctctg cccttttgct attctcaccg taacgaatca
    30001 gcggatacca tgattatcaa acgcactcct caagccgcgt cgcctctggc ttcgtggctt
    30061 tcttatctgg aaaacctgca cagtaaaact atcgatctcg gccttgagcg cgtgagccag
    30121 gtcgcggcgc gtcttggcgt cctgaaacca gcgccatttg tgtttaccgt tgcgggtacg
    30181 aatggcaaag gcaccacctg ccgtacgctg gagtcgattc tgatggcggc agggtacaaa
    30241 gtgggcgtct acagttcacc gcatctggtg cgttataccg agcgcgtacg tgtgcagggg
    30301 caggaattgc cggaatcggc ccacaccgcc tcttttgcgg agattgaatc ggcacgcggt
    30361 gatatttccc tgacctattt cgagtacggt acgctgtcgg cgttgtggct gttcaaacag
    30421 gcacaacttg acgtagtgat tctggaagta gggctgggcg gtcgtctgga cgcaaccaat
    30481 attgtcgatg ccgatgtggc tgtagtaacc agcattgcgc tggatcatac cgactggctg
    30541 ggaccagatc gcgaaagtat tggtcgcgag aaagcaggca tctttcgcag cgcaaaaccg
    30601 gcaattgtcg gtgagccgga aatgccttct accattgctg atgtggcgca ggaaaaaggt
    30661 gcactgttac aacgtcgggg cgttgagtgg aactattccg tcaccgatca tgactgggcg
    30721 tttagcgatg ctcacggcac gctggaaaat ctgccgttgc cgcttgtccc gcaaccgaat
    30781 gccgcaacag cgctggcggc actgcgtgcc agcgggctgg aagtcagtga aaatgccatt
    30841 cgcgacggga ttgccagcgc aattttgccg ggacgtttcc agattgtgag cgagtcgcca
    30901 cgcgttattt ttgatgtcgc gcataatcca catgcggcgg aatatctcac cgggcgtatg
    30961 aaagcgctac cgaaaaacgg gcgcgtgctg gcggttatcg gtatgctaca tgataaagat
    31021 attgccggaa ctctggcctg gttgaaaagc gtggttgatg actggtattg tgcgccactg
    31081 gaagggccgc gcggtgccac ggcagaacaa ctgcttgagc atttgggtaa cggcaaatca
    31141 tttgatagcg ttgcgcaggc atgggatgcc gcaatggcgg acgctaaagc ggaagacacc
    31201 gtgctggtgt gtggttcttt ccacacggtc gcacatgtca tggaagtgat tgacgcgagg
    31261 agaagcggtg gcaagtaagt ttcagaatcg gttagtgggc acgatcgtgc tggtggcgct
    31321 gggggtgatt gtacttccag ggctgctgga cgggcagaaa aaacattatc aggatgagtt
    31381 tgcggctatc ccgctggttc caaaagcggg cgatcgcgat gagcctgata tgatgccagc
    31441 cgccacccag gcgttgccga cgcagccgcc ggaaggcgca gcggaagagg tgcgggcagg
    31501 tgatgccgca gcaccgtcgc tcgatccggc cactattgca gccaataaca ccgagtttga
    31561 accggaacct gcaccggtcg ccccaccgaa gccgaaaccg gtggagccgc ctaaaccgaa
    31621 ggttgaagca ccacctgcgc cgaagccaga accgaagccg gtcgtggaag aaaaagcagc
    31681 gccaacgggt aaagcctatg ttgtgcaact gggtgcgctg aaaaatgccg ataaagtgaa
    31741 tgagattgtc ggtaagctgc gcggtgccgg ttatcgggtt tatacgtcgc catccacgcc
    31801 agtgcagggt aaaattaccc gtattctggt tgggccggat gcctcgaaag ataagctgaa
    31861 aggttcgctg ggtgagttga agcaactttc tggcttaagt ggcgtggtaa tgggctatac
    31921 gccgaattaa tacagtcttg cctgatgcga cgctggcgcg tcttatcagg cctgggcagt
    31981 gttagaaccg taggtcggat aaggcgttta cgccgcatcc gacacgcatt gcacgatgcc
    32041 gcaaaggcat aaaaagtcga tggcgttgaa tattttttca gcgccatttt tattgatgcg
    32101 tgggaaggaa atccctacgc aaacgttttc tttttctgtt agaatgcgcc ccgaacagga
    32161 tgacagggcg taaaatcgtg ggacacatat ggtctggatt gattacgcca taatcgcggt
    32221 gattgctttt tcctctctgg ttagcctgat ccgcggcttt gttcgtgaag cgttatcgct
    32281 ggtgacatgg ggttgtgctt tctttgttgc cagtcattac tacacttacc tgtcagtctg
    32341 gtttacgggc tttgaagacg aactggttcg aaatggaatt gccatcgcgg tactgtttat
    32401 cgctaccctg attgttggtg ctatcgtgaa cttcgtgata ggccagttgg tggagaaaac
    32461 ggggttgtca ggcaccgatc gggtgctggg cgtctgtttc ggtgcgttgc gcggtgtgtt
    32521 gattgttgcc gccattctct tctttctcga ctcctttacc ggggtgtcga aaagcgaaga
    32581 ctggagcaaa tcacagctga tcccgcagtt cagttttatc atcagatggt tttttgatta
    32641 tctgcaaagc tcgtcaagtt tcttgcccag agcgtaagtg ctctgagatg tggcttaacg
    32701 aggaaaaaga cgtatgtgcg gtattgtcgg tatcgccggt gttatgccgg ttaaccagtc
    32761 gatttatgat gccttaacgg tgcttcagca tcgcggtcag gatgccgccg gcatcatcac
    32821 catagatgcc aataactgct tccgtttgcg taaagcgaac gggctggtga gcgatgtatt
    32881 tgaagctcgc catatgcagc gtttgcaggg caatatgggc attggtcatg tgcgttaccc
    32941 tacggctggc agctccagcg cctctgaagc gcagccgttt tacgttaact ccccgtatgg
    33001 cattacgctt gcccacaacg gcaatctgac caacgctcac gagttgcgta aaaaactgtt
    33061 tgaagaaaaa cgccgccaca tcaacaccac ttccgactcg gaaattctgc ttaatatctt
    33121 cgctagcgag ctggacaact tccgccacta cccgctggaa gccgacaata ttttcgctgc
    33181 cattgctgcc acaaaccgct taatccgcgg cgcatatgcc tgtgtggcga tgattatcgg
    33241 ccacggtatg gttgctttcc gcgatccaaa cgggattcgt ccgctggtac tgggaaaacg
    33301 tgatattgac gagaaccgta cagaatatat ggtcgcttcc gaaagcgtag cgctcgatac
    33361 gctgggcttt gatttcctgc gtgacgtcgc gccgggcgaa gcgatttaca tcactgaaga
    33421 agggcagttg tttacccgtc aatgtgctga caatccggtc agcaatccgt gcctgtttga
    33481 gtatgtatac tttgctcgcc cggactcgtt catcgacaaa atttccgttt acagcgcgcg
    33541 tgtgaatatg ggcacgaaac tgggcgagaa aattgcccgc gaatgggaag atctggatat
    33601 cgacgtggtg atcccgatcc cagaaacctc gtgtgatatc gcgctggaaa ttgctcgtat
    33661 tctgggcaaa ccgtaccgcc agggcttcgt taaaaaccgc tatgttggcc gcacctttat
    33721 catgccgggc cagcagctgc gtcgtaagtc cgtgcgccgt aaactgaatg ccaaccgcgc
    33781 cgagttccgc gataaaaacg tcctgctggt cgacgactcc atcgtccgtg gcaccacttc
    33841 tgagcagatt atcgagatgg cacgcgaagc cggagcgaag aaagtgtacc tcgcttctgc
    33901 ggcaccggaa attcgcttcc cgaacgttta tggtattgat atgccgagcg ccacggaact
    33961 gatcgctcac ggtcgcgaag ttgatgaaat tcgccagatc atcggtgctg acgggttgat
    34021 tttccaggat ctgaacgatc tgatcgacgc cgttcgcgct gaaaatccgg atatccagca
    34081 gtttgaatgc tcggtgttca acggcgtcta cgtcaccaaa gatgttgatc agggctacct
    34141 cgatttcctc gatacgttac gtaatgatga cgccaaagca gtgcaacgtc agaacgaagt
    34201 ggaaaatctc gaaatgcata acgaaggatg atgccttcgc tgagggtgcc ggtctggcac
    34261 cctgacttgc aactcccgcc gaaatactac aaagtctgcc tgcaagtctg acagggcaac
    34321 tatttatgaa acgactcatt gtaggcatca gcggtgccag cggcgcgatt tatggcgtgc
    34381 gcttattaca ggttctgcgc gatgtcacag atatcgaaac gcatctggtg atgagccagg
    34441 cggcgcgcca gaccttatcc ctcgaaacgg atttttccct gcgcgaagtg caggcattag
    34501 ctgatgtcac gcacgatgcg cgcgatattt ccgccagcat ctcttccggt tctttccaga
    34561 cgctggggat ggtgatttta ccctgttcaa tcaaaaccct ttccggcatt gtccatagct
    34621 atactgatgg cttactgacc cgtgcggcag atgtggtgct gaaagagcgt cgcccgttgg
    34681 tgctctgcgt gcgtgaaaca ccattgcact taggccatct gcgtttaatg actcaggcgg
    34741 cagaaatcgg tgcggtgatt atgcctcccg ttccggcgtt ttatcatcgc ccgcaatccc
    34801 ttgatgatgt gataaatcag acggttaatc gtgttcttga ccagtttgca ataacccttc
    34861 ctgaagatct ctttgcccgc tggcagggcg cataactccg ctgttgccct gtttcagggc
    34921 aattttgcaa ccgcgatcaa atcctcgaca ttttgtttct gccattcaat cgaaacgctg
    34981 cgattcaacc gctatacctg ctatcttcaa cttcaggaca ataatgcaac gtcttattaa
    35041 catatttaac gttgaatgtt actgttgtcg tcaagatggc ataagacctg catgaaagag
    35101 cctgcaaaca cacaacacaa tacacaacat aaaaaagcca ttttcacttg agggttatgt
    35161 atgaagaagt cgattctcgc tctgtctttg ttagtcggtc tctccgcagc ggcttccagc
    35221 tatgcggcgc taccggagac ggtacgtatc ggaaccgata ccacctatgc gccgttctcg
    35281 tcgaaagatg ctaaaggtga ttttgttggc tttgatatcg atctcggtaa cgagatgtgc
    35341 aaacggatgc aggtgaaatg tacctgggtt gccagtgact ttgacgcgct gatcccctca
    35401 ctgaaagcga aaaaaatcga cgccattatt tcgtcgcttt ccattaccga taaacgtcag
    35461 caggagattg ccttctccga caagctgtac gccgcagatt ctcgtttgat tgctgccaaa
    35521 ggttcaccga ttcagccaac gctggattca ctgaaaggta aacatgttgg tgtgctgcag
    35581 ggatcaaccc aggaagctta cgctaacgag acctggcgta gcaaaggcgt ggatgtggtg
    35641 gcctatgcca accaggattt ggtctattcc gatctggctg caggacgtct ggatgctgcg
    35701 ttacaagatg aagttgctgc cagcgaagga ttcctcaagc aacctgctgg taaagctttc
    35761 gcctttgctg gctcatcagt aaaagacaaa aaatacttcg gtgatggcac cggtgtaggg
    35821 ctacgtaaag atgatgctga actgacggct gccttcaata aggcgcttgg cgagctgcgt
    35881 caggacggca cctacgacaa gatggcgaaa aagtatttcg actttaatgt ctacggtgac
    35941 tgatacgtcg ctgggaagct gtacctgatg gaatgatcat catggtgcac gccaggtttg
    36001 ttgcactatc gtggtgcatt gaaatgcata cttaagcatt tttaatgaaa aataatacgt
    36061 ctaacggggc ggaatatttt gccttgatgg tcaattttat ggcacgataa gtgtaacaaa
    36121 cctgtaaata ttccctataa aaagactgtc agttgaggac attatgaaaa aactggtgct
    36181 atcgctctct ctggttctgg ccttctccag cgcaactgcg gcatttgctg cgattccgca
    36241 aaacatccgc atcggtaccg acccgaccta tgcgccattt gaatcaaaaa attcacaagg
    36301 cgaactggtt ggcttcgata tcgatctggc aaaggaatta tgcaaacgca tcaatacgca
    36361 atgtacgttt gtcgaaaatc cgctggatgc gttaatcccg tccttaaaag cgaagaagat
    36421 tgacgccatc atgtcatcgc tttccattac ggaaaaacgt cagcaagaaa tagccttcac
    36481 cgacaaactg tacgctgccg attctcgttt ggtggtggcg aaaaattctg acattcagcc
    36541 gacagtcgag tcgctgaaag gcaaacgggt aggcgtattg cagggcacca cccaggagac
    36601 gttcggtaat gaacattggg caccaaaagg cattgaaatc gtctcgtatc aggggcagga
    36661 caacatttat tctgacctga ctgccggacg tattgatgcc gcgttccagg atgaggtcgc
    36721 tgccagcgaa ggtttcctca aacaacctgt cggtaaagat tacaaattcg gtggcccgtc
    36781 tgttaaagat gaaaaactgt ttggcgtagg gaccggcatg ggcctgcgta aagaagataa
    36841 cgaactgcgc gaagcactga acaaagcctt tgccgaaatg cgcgctgacg gtacttacga
    36901 gaaattagcg aaaaagtact tcgattttga tgtttatggt ggctaatcgc catcggtgaa
    36961 agaaagcccg ctcccttcgg gtaaccggag ggagacaaga ggcggtaatt caccacacac
    37021 gacaggacag gcagcatgtt gtatgggttt tcaggtgtta ttttacaggg tgcgctcgtc
    37081 acgctggagc tggctatcag ctctgtagtg ctcgctgtaa tcatcggttt aattggcgct
    37141 ggcggtaagc tctcgcaaaa tcggctttcg gggctgattt tcgaagggta caccacgctg
    37201 attcgtggcg tgccggactt agtgttgatg ctgctgattt tctacggttt gcagattgcg
    37261 ctaaacacgg tgacggaggc gatgggcgtc gggcagattg atatcgatcc gatggtcgct
    37321 ggtattatca ctctcggttt tatctacggt gcttatttta ccgaaacgtt ccgtggtgct
    37381 tttatggcag tgccgaaagg acatatagag gcggcgacgg cgttcggttt tactcgtggg
    37441 caagtgtttc ggcggatcat gtttccggcg atgatgcgtt acgccctgcc aggcattggc
    37501 aacaactggc aggtgatcct caaatctacc gcactggttt cgttactcgg cctggaagat
    37561 gtggtcaaag ccacccaact ggcaggcaaa agtacctggg aaccgttcta tttcgccatc
    37621 gtctgtggcg tgatttacct ggttttcacc actgtttcca atggtgtgct gctgttcctt
    37681 gagcgccgct actccgtggg tgtgaagagg gctgacctgt gatcgaaatc ttacatgaat
    37741 actggaaacc gctgctgtgg accgacggtt atcgctttac tggtgtggcg atcactctgt
    37801 ggctgcttat tttgtcggta gtgataggcg gagtcctggc gctgtttctg gcgattggtc
    37861 gtgtctccag taataaatac atccagtttc caatctggtt atttacctat atttttcgcg
    37921 gtacgccgct gtatgttcag ttgctggtgt tctattccgg catgtacacg cttgagattg
    37981 ttaagggaac cgaattcctt aacgctttct tccgcagtgg cctgaactgt accgtgctgg
    38041 cgctgacgct taacacctgc gcttacacta ccgagatttt tgctggggca atccgttcgg
    38101 ttccgcatgg ggaaattgaa gccgccagag cctatggctt ctcgactttt aaaatgtatc
    38161 gttgcattat tttgccttct gcgctgcgta ttgcgttacc ggcatacagc aacgaagtga
    38221 tcctgatgct gcactctact gcgctggcgt ttacggccac tgtgccggat ctgctgaaaa
    38281 tagcccgcga tattaacgcc gccacgtatc aaccttttac cgccttcggc attgccgcgg
    38341 tgctctattt aatcatctct tatgtcctga tcagcctctt tcgcagagcg gaaaaacgct
    38401 ggttgcagca tgtgaaacct tcttcaacgc actgagaata cgatgtccga gaataaatta
    38461 aacgttatcg atttgcacaa acgctacggc gaacatgaag tgctgaaagg ggtctcactg
    38521 caagcgaatg ccggagatgt aataagcatc atcggatcgt cgggatcggg gaaaagtacc
    38581 tttctgcgct gcattaactt cctcgaaaaa ccgagtgaag ggtcgatcgt ggtcaatggc
    38641 cagacgatca atctggtgcg cgacaaagac ggtcaactca aagtcgccga taaaaatcaa
    38701 ctgcgcttac tgcgcacacg cctgacgatg gtattccagc acttcaatct ctggagccat
    38761 atgacggtgc tggaaaacgt catggaagcg ccgattcagg tgttgggcct gagcaagcag
    38821 gaagcgcgcg agcgggcggt gaagtatctg gcgaaagtcg ggatagacga acgtgcgcag
    38881 gggaaatatc cggtgcatct ttccggcggt cagcaacagc gtgtttctat cgcgcgggcg
    38941 ctggcgatgg aaccggaagt tttactgttt gatgaaccta cctcggcgct cgatcctgaa
    39001 ctggtaggcg aagtgttacg tattatgcag caactggcag aagaggggaa aaccatggtg
    39061 gtagtgactc acgaaatggg ctttgctcgc catgtttcta ctcacgtcat tttcctccat
    39121 caggggaaaa tagaagaaga gggcgcgccg gagcagttat ttggcaaccc gcaaagccct
    39181 cgtctgcaac agttccttaa gggatcgctg aaataatttc ctctccggtg gctaacgctg
    39241 ccggagtatt ttcatgggga atttgcgagt cagcttccgc tgaaatgtaa aagttgcagt
    39301 ccgctaaaaa tacttctgaa ttcgctgtcc aaagataaaa actgcctgct gcattgggtg
    39361 ttaatcgtgg ttattgtcgc gtcatcttaa atttgagggg tgacggaacg ccatgacaat
    39421 atcgacaact tccacgccgc atgatgcggt atttaaatct tttttacgcc atccaga
//
LOCUS       DAJTVK010000025        39423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000025 DAJTVK010000000
VERSION     DAJTVK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.025"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..267
                     /locus_tag="OO372_001363"
     CDS             <1..267
                     /locus_tag="OO372_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6340906.1"
                     /translation="ETKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGV
                     GLKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            361..759
                     /gene="fadM"
                     /locus_tag="OO372_001364"
     CDS             361..759
                     /gene="fadM"
                     /locus_tag="OO372_001364"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6340907.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(811..1506)
                     /gene="queC"
                     /locus_tag="OO372_001365"
     CDS             complement(811..1506)
                     /gene="queC"
                     /locus_tag="OO372_001365"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6340908.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1571..3271)
                     /locus_tag="OO372_001366"
     CDS             complement(1571..3271)
                     /locus_tag="OO372_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR6340909.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3371..4189
                     /gene="cof"
                     /locus_tag="OO372_001367"
     CDS             3371..4189
                     /gene="cof"
                     /locus_tag="OO372_001367"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6340910.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4341..4799
                     /gene="decR"
                     /locus_tag="OO372_001368"
     CDS             4341..4799
                     /gene="decR"
                     /locus_tag="OO372_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6340911.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4829..6601
                     /locus_tag="OO372_001369"
     CDS             4829..6601
                     /locus_tag="OO372_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6340912.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6594..8375
                     /locus_tag="OO372_001370"
     CDS             6594..8375
                     /locus_tag="OO372_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6340913.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8556..8894
                     /gene="glnK"
                     /locus_tag="OO372_001371"
     CDS             8556..8894
                     /gene="glnK"
                     /locus_tag="OO372_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6340914.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8924..10210
                     /gene="amtB"
                     /locus_tag="OO372_001372"
     CDS             8924..10210
                     /gene="amtB"
                     /locus_tag="OO372_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6340915.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10259..11119)
                     /gene="tesB"
                     /locus_tag="OO372_001373"
     CDS             complement(10259..11119)
                     /gene="tesB"
                     /locus_tag="OO372_001373"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6340916.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11337..11909
                     /locus_tag="OO372_001374"
     CDS             11337..11909
                     /locus_tag="OO372_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6340917.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11940..12251)
                     /locus_tag="OO372_001375"
     CDS             complement(11940..12251)
                     /locus_tag="OO372_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6340918.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12414..12510
                     /gene="ffs"
                     /locus_tag="OO372_001376"
     ncRNA           12414..12510
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO372_001376"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12630..12983
                     /locus_tag="OO372_001377"
     CDS             12630..12983
                     /locus_tag="OO372_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6340919.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13025..14573)
                     /gene="pdeB"
                     /locus_tag="OO372_001378"
                     /pseudo
     CDS             complement(13025..14573)
                     /gene="pdeB"
                     /locus_tag="OO372_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14737..15207)
                     /locus_tag="OO372_001379"
     CDS             complement(14737..15207)
                     /locus_tag="OO372_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6340920.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15323..15874)
                     /gene="maa"
                     /locus_tag="OO372_001380"
     CDS             complement(15323..15874)
                     /gene="maa"
                     /locus_tag="OO372_001380"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR6340921.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16045..16263)
                     /gene="hha"
                     /locus_tag="OO372_001381"
     CDS             complement(16045..16263)
                     /gene="hha"
                     /locus_tag="OO372_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6340922.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16289..16663)
                     /gene="tomB"
                     /locus_tag="OO372_001382"
     CDS             complement(16289..16663)
                     /gene="tomB"
                     /locus_tag="OO372_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6340923.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17209..20358)
                     /locus_tag="OO372_001383"
     CDS             complement(17209..20358)
                     /locus_tag="OO372_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6340924.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20381..21574)
                     /gene="acrA"
                     /locus_tag="OO372_001384"
     CDS             complement(20381..21574)
                     /gene="acrA"
                     /locus_tag="OO372_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6340925.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21716..22363
                     /gene="acrR"
                     /locus_tag="OO372_001385"
     CDS             21716..22363
                     /gene="acrR"
                     /locus_tag="OO372_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6340926.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22491..25853
                     /gene="mscK"
                     /locus_tag="OO372_001386"
     CDS             22491..25853
                     /gene="mscK"
                     /locus_tag="OO372_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6340927.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26065..26226)
                     /gene="rsmS"
                     /locus_tag="OO372_001387"
     CDS             complement(26065..26226)
                     /gene="rsmS"
                     /locus_tag="OO372_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6340928.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26240..26767)
                     /gene="priC"
                     /locus_tag="OO372_001388"
     CDS             complement(26240..26767)
                     /gene="priC"
                     /locus_tag="OO372_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6340929.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26837..27214
                     /locus_tag="OO372_001389"
     CDS             26837..27214
                     /locus_tag="OO372_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6340930.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27367..27918
                     /gene="apt"
                     /locus_tag="OO372_001390"
     CDS             27367..27918
                     /gene="apt"
                     /locus_tag="OO372_001390"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6340931.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28047..29972
                     /gene="dnaX"
                     /locus_tag="OO372_001391"
     CDS             28047..29972
                     /gene="dnaX"
                     /locus_tag="OO372_001391"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6340932.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29305..29369
                     /gene="dnaX"
                     /locus_tag="OO372_001391"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30025..30354
                     /locus_tag="OO372_001392"
     CDS             30025..30354
                     /locus_tag="OO372_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6340933.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30354..30959
                     /gene="recR"
                     /locus_tag="OO372_001393"
     CDS             30354..30959
                     /gene="recR"
                     /locus_tag="OO372_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6340934.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31069..32943
                     /gene="htpG"
                     /locus_tag="OO372_001394"
     CDS             31069..32943
                     /gene="htpG"
                     /locus_tag="OO372_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6340935.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33124..33768
                     /gene="adk"
                     /locus_tag="OO372_001395"
     CDS             33124..33768
                     /gene="adk"
                     /locus_tag="OO372_001395"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6340936.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33900..34862
                     /gene="hemH"
                     /locus_tag="OO372_001396"
     CDS             33900..34862
                     /gene="hemH"
                     /locus_tag="OO372_001396"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6340937.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34859..35818)
                     /gene="aes"
                     /locus_tag="OO372_001397"
     CDS             complement(34859..35818)
                     /gene="aes"
                     /locus_tag="OO372_001397"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR6340938.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35970..37274
                     /gene="gsk"
                     /locus_tag="OO372_001398"
     CDS             35970..37274
                     /gene="gsk"
                     /locus_tag="OO372_001398"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6340939.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37407..39083)
                     /locus_tag="OO372_001399"
     CDS             complement(37407..39083)
                     /locus_tag="OO372_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6340940.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9529 a        10050 c        10425 g         9419 t
ORIGIN      
        1 gaaaccaaag cggaagctcc tgcagcacaa agtaaagcag cagtaccggc gaaagccagt
       61 gacgaagaag gcacccgggt cagcattaat aatgccagcg cggaagagct agcccgcgcg
      121 atgaatggcg ttggcctgaa gaaagcgcag gcgattgtca gttatcgcga agagtacggt
      181 ccgtttaaaa cggtggagga tctaaagcag gtgccgggga tgggcaattc gctggtggaa
      241 cgtaatctgg cggtattaac cctgtaatta atttgcatag tggcaatttt ttgccagact
      301 gaagaggtca taccagttat gacctctgta cttataacaa caacgtaagg ttattgcgct
      361 atgcaaacac aaatcaaagt tcgtggatat catctcgacg tttaccagca cgtcaacaac
      421 gcccgctacc ttgaatttct cgaagaagcc cgctgggatg ggttggaaaa tagcgacagt
      481 tttcagtgga tgacggccca taacatcgcc ttcgtcgtgg tcaatatcaa tattaactat
      541 cgtcgcccag cggtattaag cgacctgtta actattacca gtcagttgca gcaattaaac
      601 ggtaaaagcg gcatcttaag ccaggtcatt acactggagc cggaagggca ggtggtagcg
      661 gatgcgctta ttacgtttgt ttgtattgat cttaaaacgc agaaagcatt agctctggaa
      721 ggggaattgc gcgaaaagct ggagcagatg gttaagtaaa cgttttgtgg tgccggatgc
      781 tcaagccgca tccggcgaca cccggaataa ttacctcaac ccggttttct gcttcatcgc
      841 tgccatcacc gtcggtttat cggccagata atgattcaaa ccgttggcgc gtaaattaca
      901 tgccgcacaa tgaccgcaac cgtcgccttt aatgccgtta tagcaggtca acgtttcgtt
      961 acggactaaa tccagtttgc cgtaataatc tgccagcgcc caggtttccg ctttatcaat
     1021 ccacatcagc ggcgtttcaa aacgaatatc tttcgccatg cccaaactga cggcatggtt
     1081 tagtgctttc acaaactcat cgcggcaatc cgggtagccg gagaaatccg tttcacagac
     1141 gccggtaatt acggcttctg cttttacctg atacgcatat attgccgcca gcgtcaggaa
     1201 caaaatatta cgcccaggga caaacgtatt cgggatgcca tcggcttcag gttcataatc
     1261 aggcaccgga atgctgtcac gtgtcaggct gctgaccgcc agctcgttga gcagcgttac
     1321 atcaagaacc ttatgcgcgc gtgctcccag tttcagcgcc agttcgcgtg ccacgtcgat
     1381 ttctgcgcga tgtcgctgac cgtaatcgaa cgtcacgcaa tggacttcat catattgttg
     1441 taatgcctgc accagacagg tggtggaatc ctggcctcca ctgaacacaa cgacagcacg
     1501 tttcatagat aatcctgctt gaacaataaa agcgttatgg taacgcctgc gattaacccg
     1561 gaccagctat tcattgcgac ggcgcgggaa gccaggcttc ggtaaattca aaccagccgc
     1621 gcggtgtcag tcgcacaccg ttcacgccgg gaggggcact aatgcgatag tgatagttga
     1681 acagcggcgt cagcgtcgcg tctgccatta actggctaaa aaccgctttc agggcattaa
     1741 atcggttttc ctcatcaggc attacttgca ccgcgtccag cgtcgattgc agatgtgcat
     1801 atgctggagc gtcgaaaaca tgtggccata gtggatcgca acgtagccat tgctccagag
     1861 tatattccgg tgcttcgcca attaatctgt cgcccatcat gaggtctgcg tgtgccagta
     1921 gggtcgtgtc gtcccagttt tttgcgttat gaaaaataat tgtgagttca cagccttccg
     1981 ctgccagtgt cgcctgtagg cgttctgcca tggtatgaag ttctatcggt aggtgataaa
     2041 ccagcgtcaa ggttttcggt agtttgactt catccggtac ttgccagtgc ggaatagtcc
     2101 agcctggcag taatgcatga ctggcggtga tcaggttttc tccgacttct aacgtttgta
     2161 ataaaccgga ttgatgaata atggagatca cttttcgcgc ctgccagagg gagagtcggg
     2221 gacttttgcg caacgttaaa tagcaaaaac ctaaactgat gccactactg acctggctga
     2281 cccgttgcag ctcctccggt ttgccgatgg tgatttgcac gggatgccga caactggttc
     2341 ccaaatcttt ttcgaaaagc ggcggagtta tccagtactc aaccgcttta agcagcggat
     2401 gacgcaaatg gtaataatca tggctctcca ggcgcaccag ctccgctgtg aattgtgtta
     2461 atcgaaaagg accagtgccg atcagtggga attgcggatg cgccagatgg ctgcaatagc
     2521 tcgccagccg gtgcgcaagc cagtaatcgg ggcgatgtaa aaagaaggtc agacactgcg
     2581 gatgggtgac ttcaatacgc ttcacgctaa taaataattg atccagtgct ggcagttgta
     2641 acagcatcaa taatcgctgg tgtaagtgtg aggcttttac tgcatcgccg ttatgccagt
     2701 gtagggttga acgaagataa aagtcccagc gtaacccgtc agtagaggtt tccctgtgat
     2761 gcgctaaatc gccaatcggg cgctgagtat tattatcgaa gcgggtcagg ccggaaaata
     2821 tctgcccggc gagatgctgc tcggcacggc cgggcaaaaa gcctggttgt agcggttcga
     2881 gcgggcgata gtagggaata cgcaacgtgg gtgtatcgtt ttgccattgt ccgcccataa
     2941 acggctgtaa cagagtgcgc agctcacctg gggccagttg cgccagctcc agcacatctt
     3001 gctgctttcc ggtttccagt gcctgttcca tcatcgcatt gcgtagcgat tccggcgtga
     3061 ccagaaagcg taattgtccg cgctttccgc gtcctgactg cgcctgccac tccagccatc
     3121 ccgcctcctg tgcctgacgc aacagcgtac gaacatggcg ttcgctgcaa aaacagcgtt
     3181 cggccagttc gctgacggtg acggtttgcg gctttccggc tgaaggttgc cacagacgtt
     3241 gatactggtt aagacggttg agcaatcgca tataaacccg gaacaatatt atttaactat
     3301 tcactattac ttccgtatat atcaggtgat actcaatctc cattaaccgt gtcacagagt
     3361 ggagaaagaa atggctcgtc tggcagcatt tgatatggat ggcactttat tgatgcctga
     3421 ccatcattta ggtgagaaaa ccctctctac tttggcgcga ctgcgtgaac gcgacattac
     3481 cctcactttt gccacggggc gtcatgcgct ggagatgcag catattctcg gggcgctatc
     3541 gctggatgca tatttgatta ccggcaacgg aacgcgcgtg cattctctgg aaggtgaact
     3601 tttacatcgt gatgatttac ctgcggatgt cgcggagctg gtgctgtatc agcaatggga
     3661 tacccgagcc agcatgcata tcttcaatga cgacggttgg tttaccggga aagagatccc
     3721 tgcgttgttg caggcatttg tctatagcgg ttttcgttat cagataatcg atgtcaaaaa
     3781 aatgccactc ggcagcgtca ccaagatctg cttctgtggc gatcacgacg atcttacacg
     3841 cttgcagatc cagctatacg aagcattagg cgagcgtgca catttgtgtt tttccgccac
     3901 agattgcctc gaagtgctgc cggtgggctg caataaaggc gctgcattga cggtgctgac
     3961 ccaacattta ggtttatcgt tgcgcgattg catggccttt ggtgatgcga tgaacgatcg
     4021 cgaaatgtta ggcagcgtcg gtagcgggtt tattatgggc aatgcgatgc cgcaactgcg
     4081 cgcggagctc ccgcatttac cggtgattgg acattgccga aatcaggctg tctctcacta
     4141 tttgacgcac tggctggact atccacatct accttattcc cccgaataac gagatccctt
     4201 ccagcaccgg gcaattgccc ggtttttttg cgttgaattt gtcattttgt gccgtggtgt
     4261 ttaaaccgca cagaataaat tgtcgtgatt tcacctttaa aatagaatta aaagagaaaa
     4321 aaattctccg tggaagggct atgttagata aaattgaccg taagctgctg gccttactgc
     4381 agcaggattg caccctctct ttgcaggcac tggctgaagc cgttaatctg acaaccaccc
     4441 cttgctggaa gcgcctgaaa cggctggagg acgacggtat ccttatcggc aaagtcgccc
     4501 tgctggatcc ggaaaaaata ggcctcggcc tgaccgcctt tgtgctgata aaaacgcaac
     4561 atcacagcag cgaatggtat tgccgctttg tcacggtggt taccgaaatg ccagaagtgc
     4621 tggggttctg gcgcatggct ggtgaatacg attatctgat gcgcgtccag gttgccgaca
     4681 tgaaacgcta cgacgagttt tataagcgtc tggtaaacag cgtgccgggg ctgtcggacg
     4741 tcacttccag cttcgcgatg gaacagatta aatacaccac ttctttaccc atcgaataaa
     4801 tatccagaat caggtcagga cacaacgcgt gcgattattt gctcaattaa gctggtattt
     4861 ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg cttgtcatta tcgcgatgct
     4921 gcaactggtt ccgccaaaag tggttggtat tgttgtcgat ggcgtgacag aacaacactt
     4981 tactaccggg ctgatcctga tgtggatcgc caccatggtg ctgattgccg ttgtggttta
     5041 tctcctgcgt tatgtctggc gcgtattgct gtttggcgcg tcttaccaac tggctgttga
     5101 actgcgtgaa gattattacc gtcagctaag ccggcagcac ccggagtttt acctgcgtca
     5161 tcgcacgggt gatctcatgg ctcgtgcgac caatgacgtc gattgcgtcg tgtttgccgc
     5221 cggagaaggg gtgctgacgc tggtggattc actggtaatg ggctgcgctg tgttgattat
     5281 gatgtctacg caaattagct ggcagttgac cttatttgcc ctgttgccga tgccagtgat
     5341 ggcgatcatg attaagcgca acggcgatgc tttgcatgaa cgctttaagc tggcacaggc
     5401 ggcgttttcc agtcttaatg accgcaccca ggaaagcctc accagtatcc gcatgatcaa
     5461 agcctttggt ctggaagatc gccagtcggc gttatttgcc gcggatgccg aagataccgg
     5521 caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc gacccgacca tctatatcgc
     5581 gattggtatg gcgaacttgc tggcgattgg cggcggtagt tggatggtgg tgcagggcag
     5641 tttaacgctg ggccagctca ccagttttat gatgtattta ggtctgatga tttggccaat
     5701 gctggcgctg gcatggatgt ttaacattgt ggaacgtggt agtgctgcgt acagccgtat
     5761 tcgtgcgatg ctggcggaag cgccggtggt gaacgatggt agcgaacccg tgccggaagg
     5821 gcgtggcgaa cttgatgtaa atattcacca gttcacgtat ccgcagactg accatcctgc
     5881 gctggaaaac gtcaatttcg ccctgaaacc cggtcagatg ctgggtatct gcgggccgac
     5941 tggttccggc aaaagtaccc tgttgtcgct cattcagcgt catttcgacg ttagcgaggg
     6001 ggatattcgc tttcatgata ttcctctgac gaagttacaa ctcgatagct ggcgtagccg
     6061 cctggcggtg gtcagccaga cgccattcct tttttctgac accgtggcaa ataacatcgc
     6121 gctgggttgc ccgaatgcca cccagcaaga gattgagcat gtcgcgcggt tagccagcgt
     6181 acatgacgat attttgcgtt taccgcaagg ttacgataca gaggtgggcg agcgcggcgt
     6241 gatgctttcc ggcgggcaaa aacagcgtat ctctattgct cgtgcgttat tagtcaacgc
     6301 ggaaatcctc atccttgatg atgcgctttc ggcggtggac ggacgcacag agcaccagat
     6361 cctgcataac ctgcgtcagt ggggacaggg aagaacggta atcatcagtg cccatcgcct
     6421 ttcggcactg acggaagcca gtgaaattat tgtgatgcag cacggacata tcgcccagcg
     6481 tggcaatcat gatgtgctgg cgcaacaaag cggctggtat cgcgatatgt atcgctatca
     6541 acaactggag gcggcgctcg acgacgctcc ggaaaatcac gaggaggccg tcgatgcgta
     6601 gttttagcca actgtggccg actctcaagc gcctgttagc gtacggttca ccgtggcgta
     6661 aaccgctggg gattgcggtc ctgatgatgt gggttgcggc ggcggcagaa gtcagtgggc
     6721 cgctgcttat cagctatttt atcgacaata tggtagcgaa aaataacctg ccgttgaaag
     6781 tggttgcagg gctggctgcg gcgtatgttg ggctacaact gtttgccgcc gggctacatt
     6841 acgcgcagtc gctgctgttt aatcgggcgg cagtaggcgt agtgcaacag ttgcgtaccg
     6901 acgtgatgga tgctgcgtta cgccagccat taagcgagtt tgatacccaa cccgtcgggc
     6961 aggtgatttc ccgcgtcact aatgacactg aagtgatccg cgatctctac gttaccgtag
     7021 tggcaactgt cctgcgcagt gccgcactgg tgggcgcgat gctggtggcg atgttcagcc
     7081 tcgactggcg aatggcgctg gtggcgataa tgattttccc ggtagtgctg gtggtaatgg
     7141 tgatatatca acgttacagc acgccgattg tccgtcgtgt gcgcgcctat ctggcggata
     7201 tcaacgacgg ctttaacgaa atcatcaatg gcatgagcgt catccagcag ttccgtcagc
     7261 aggcgcgatt tggcgaacgt atgggtgagg ccagtcgttc acactatatg gcgaggatgc
     7321 aaaccctgcg cctcgacggt tttctgctgc gtccgctgct gagtctgttt tcatcgctca
     7381 ttctttgtgg attgttgatg ctgtttggct tctccgccag cggcaccatt gaagtgggcg
     7441 tgctgtatgc ctttatcagc tatcttgggc gacttaacga acccttaatc gaactgacca
     7501 cgcaacaggc gatgctgcaa caggctgttg ttgctggtga gcgcgtgttt gaactgatgg
     7561 acggaccgcg ccagcaatat ggcaatgatg attacccgtt acagagtggc accatcgaag
     7621 tcgataacgt gtcatttgct tatcgcgatg acaatctggt gctaaagaac attaatctct
     7681 ctgtgccttc gagtaacttt gtggcgctgg tcgggcatac cggcagtggt aaaagtaccc
     7741 tcgccagttt attgatgggc tattacccgc taacggaagg tgagattcgc cttgatggtc
     7801 gtccattaag ttcgctaagt cacagcgcgc tgcgccaggg cgtggcaatg gtgcagcaag
     7861 atccggtggt gctggcggat accttcctcg ccaacgtgac gctggggcgg gatatctccg
     7921 aagaacgcgt ctggcaggcg ctggaaaccg tgcaactggc ggagctggcg cgtagcatga
     7981 gcgacggtat ttacacgccg ctgggcgagc aggggaataa tctctcagtt gggcaaaagc
     8041 aactgctggc actggcgcgc gtgctggtcg agacgccgca aatcctgatc cttgatgagg
     8101 caaccgccag cattgactcc gggactgaac aggcgattca acatgctctg gcggcggtgc
     8161 gtgaacatac cacgctggtg gtgattgctc accgcttatc gaccattgtt gatgccgaca
     8221 ccattctggt gcttcatcgt gggcaagccg tggagcaggg cactcaccag caactgctgg
     8281 ctgcccaggg acgctactgg cagatgtatc aactgcaact tgcgggcgaa gagctggcag
     8341 ccagcgtgcg tgaagaggaa tcattgagcg cctgaatagc gcaatatttc atcgttggtg
     8401 caaaaatgta acgcactgtg cactgtcata gtgcgttttc attttcaaac ttcttaactt
     8461 cctgctctct ttctcgtttt tcatttctgg cacaccgctt gcaatacctt cttcgtgtag
     8521 cagaaccatt accgaattct gaccggaggg gatctatgaa gctggtgacc gtgataatca
     8581 aaccattcaa gctggaagac gttcgtgaag cgttatcttc cattggtatt cagggcctga
     8641 ccgtcaccga agtgaaaggt ttcgggcgtc agaaagggca tgccgagctg taccgggggg
     8701 cggaatacag cgtcaacttc ctgccaaaag taaaaattga tgtggcgatt gctgatgacc
     8761 aactcgatga agtgatcgat atcgtcagta aggcggctta caccggaaaa attggcgacg
     8821 gcaaaatctt cgtcgctgaa ttgcaacgcg tcattcgtat tcgtaccggc gaagccgacg
     8881 aagcggcgct gtaatctctg gcacacagca acaggaacga aaaatgaaga tagcgacgat
     8941 aaaaactggg cttgcttcac tggcgatgct gccgggactg gtaatggctg cacctgcggt
     9001 ggccgataaa gccgacaatg cgtttatgat gatttgtact gcgctggtgc tgtttatgac
     9061 tattccgggg attgccctgt tttacggtgg gttgattcgc ggcaaaaacg tgctgtcgat
     9121 gctgacgcag gtgacggtga cgtttgcact ggtctgtatt ctctgggtgg tttacggtta
     9181 ctcgctggcg tttggtgagg gcaacaactt cttcggcaac attaactggt tgatgctaaa
     9241 aaacatcgaa ctgacggcgg tgatgggcag catttatcag tatatccacg tggcgtttca
     9301 gggctcgttt gcctgcatta ccgtcggctt gatcgttggg gcgctggcgg aacgaatccg
     9361 cttctcagct gtgttgattt tcgtggtggt atggctgacg ctctcttaca ttccgattgc
     9421 gcatatggtg tggggcggtg gtttgctggc ttctcacggt gcgctggatt tcgcgggtgg
     9481 cacggtggtg catattaacg ccgcaatcgc cggtctggtg ggcgcgtatc tgataggcaa
     9541 acgcgtgggc ttcggcaaag aggcgtttaa accgcacaac ctgccaatgg tcttcaccgg
     9601 tactgccatt ctctatattg gttggtttgg ctttaacgcc gggtcagcgg gcacggcgaa
     9661 tgaaatcgcg gcactggcat ttgtgaatac tgtggtcgca acggcggcgg caattcttgg
     9721 ctggatcttc ggtgaatggg cgctgcgtgg taagccttct ctgctgggag cgtgttctgg
     9781 cgcgattgcc ggtctggtcg gcgtgacgcc agcgtgtggc tacattgggg ttggcggcgc
     9841 gttgattatc ggcgtggtag ctggtctggc gggcttgtgg ggcgttacta tgctcaaacg
     9901 cttgctgcgg gtggatgatc cctgcgatgt cttcggtgtg cacggcgttt gtggcattgt
     9961 cggctgtatc atgactggga tttttgccgc cagttcgctg ggtggcgtgg gcttcgctga
    10021 aggtgtgacg atgggccatc agttgctggt acagctggaa agcatcgcca ttacgatcgt
    10081 ctggtccggt gtggtggcat ttattggcta caaactggcg gatctgacgg ttggtctgcg
    10141 tgtgccggaa gaacaggagc gagaagggct ggacgtcaac agccacggcg agaatgccta
    10201 taacgcgtaa caagcactgc aaaaaacagc cggacggttt tcacctccgg ctattttttt
    10261 aattgtgatt acgcatcacc ccttcctgaa cggtcgaggc aaccagcacg ccgtcctgag
    10321 tataaaactc accgcgcaca aagccgcgtg cgctggacgc cgaggtgctc tccacgctat
    10381 acagcagcca ttcattcaaa ttaaacgggc gatgaaacca catggaatgg tcaatggtgg
    10441 caatctgaat ccccggttcg agaaaaccga tgccgtgcgg ctgtagagct accggcagga
    10501 agttaagatc agaagcgtaa ccgagcagat actgatgaac gcgcaggtca tccggcacgc
    10561 taccatttgc gcggatccac acctgacgat gtggttctgc gacgtgacct ttcagtgggt
    10621 tatgaaactc caccggacgg acttccagcg gacgatcgca gatgaattta tctttcagca
    10681 ctggcggcag caggtgcgcc agcgattggg cgatttgtgt ttccgaaggc aggccatcag
    10741 gcgctggcgc ggacggcatt gttttttgat gttcgaaacc cgcttcaggt gcctggaaag
    10801 aggccgtcat ataaaaaatc ggtttgccgt tttgaatagc agcaacccgg cgggcgctga
    10861 agctgttacc gtcacgcagc gtttcgacat cataaataat cggcttctta ctatcgccag
    10921 ggcgaagaaa gtagctgtga aacgaatgta ccagccgctc ttcagggacg gtctcttttg
    10981 cagcatacaa ggcctgaccc acgacctggc cgccaaacac ctggcgtaaa cctaaatctt
    11041 cactctggcc gcgaaagagt ccttcctcaa ttttttccag atttaacaat gtcagtaaat
    11101 tttttagcgc ctgactcata taactctcca gtaactaagc tgccgcagca agccaaagtg
    11161 agttgagtat aacgcaaatt tgccactggt ccgatgggtg caatagtctg aattacgggc
    11221 taattacagg cagaaatgcg tgatgtgtgc cacacttgtt gacgttacta ttttgttaac
    11281 cactcttccg gcgaggaaag ttagcccgct ggtgcattga taataaggag aagtgaatga
    11341 aactcgtgca catggccagt tgtttagcgg ttgcgattgc gttggcggct tgcgcagata
    11401 aaagcgcgga tattcagacg ccagcaccgg cggcaaatac gtctatttca gcaacacaac
    11461 aacctgctat ccagcaaccg aatgtctccg gtaccgtttg gatccgtcag aaagtcgcac
    11521 tgccgcctga tgctgtgctg accgtgacac tttctgacgc gtcgttagcc gatgcgccgt
    11581 caaaagtgct ggcgcagaaa gcggtgcgta ccgaaggtaa acagtcacca ttcagctttg
    11641 ttctgccttt taacccggca gatgttcagc cgaacgcgcg tattctgttg agtgcggcga
    11701 ttaccgtgaa tgacaaactg gtatttatta ccgataccgt tcagccggtg atcaacaagg
    11761 gcggaactaa agccgacctg acattggtgc cgatgcagca aaccgccgtg ccggttcagg
    11821 ccagcggtgg tgcaacgact accgtacctt cgacttcacc aactcaggtg aatccgtctt
    11881 cggcagttcc agctcctacg caatattaag cgacgttaac cctctccgcc cggagagggt
    11941 tagtagttcc agcgataacg ctgcaagtcg atttgcccgc ttcccgatac catcacacct
    12001 tctgccagta atgcctgtcg ctgacgctgt aaatccggtc cggttagcga aattgtgccg
    12061 tggcgattaa ccacccggtg ccagggtaag gtgctgcctt cagggagacg ctttaacaca
    12121 ccgcccacct ggcgcgcggc gcggggcgat cccgccagtt tcgccacatc accgtaagtg
    12181 gtgacatagc cttcgggaat agcggcgacg atttgccaga cgcgttgggg aaatgaatct
    12241 tctttttcca tcttttcttc ctgaggtaat ttttcagcat aatctggaaa aacgcccgag
    12301 tgaagtcgca ttgcgcaaga aaccagcatc tggcacgcga tgggttgcaa ttagccgggg
    12361 cagcagtgat aatgcgcctg cgcgttggtt ctcaacgctc tcaatggggg ctctgttggt
    12421 tctcccgcaa cgctactctg tttaccaggt caggtccgga aggaagcagc caaggcagat
    12481 gacgcgtgtg ccgggatgta gctggcaggg cccccaccca tttctgcctc ccaccgtttc
    12541 gtcaaaaaat cccaacatag ctaaacttta accacaactg acgtcgcaag aattgtctgg
    12601 ctgcgcagta cgcttcggag gtatgtctga tgaagtatgt tgatggtttt gtggttgccg
    12661 ttcctgccga taaaaaggat gcctatcggg aaatggccgc taaggctgcg ccattgttta
    12721 aagagtttgg cgcgcttcgt attgtcgaat gctgggccag cgatgtaccg gatggcaaag
    12781 tgaccgattt tcgtatggcg gtgaaagcgg aagagaatga agaggttgtc tttagctgga
    12841 ttgaataccc ttcaaaagag gtccgcgacg ctgctaatca aaagatgatg tcggacccac
    12901 ggatgaaaga gttcggcgag tccatgccgt ttgacggcaa gcgaatgatc tatggcggat
    12961 tcgagtcaat catcgacgaa taggtagcgt gacgggctgt gcggcgtgcc gtcagcccaa
    13021 aaattcacaa atgttgctcg gcccagcgta agaaatcttc tttcggtaat gccttgctgt
    13081 agagccagcc ctgaccgtag tgcacgccat gctggcgtag ccactcttct tgtttactgg
    13141 tttcgattcc ctccgctacc attttcagtt tcagtgtttt tgccatttcg atgatatgcg
    13201 gcgtgacatt tttatattcc agcgcatcaa cgaaagattt atcgatcttc agaatgtcga
    13261 catccagatc ctgtaaataa cttaaacttg aatatcccgt accaaaatca tcaagataaa
    13321 tttcatggcc cgcctcccgg tagcgagaaa ttatcggggc gctggttttc ggatcggcaa
    13381 actcgcgttc agtgagttca agcgcgatct gtctgggatt aacctgattg attgatcatt
    13441 tcacgcagca attgcgggat tttttccgag gtgagcacgg tggattcaag attgatcgaa
    13501 atatgctgct gtggatgctg acgcagccag tcgcccatat cttcaaaggc gcttcttata
    13561 atcagtagcg tcaatggctc agaaaggccc gtttgctgtg ccagcggaat aaaactatct
    13621 ggtgacaacc aactaccgtc tgtctgcggc cagcgcgcca gtgcctcagc accgacaatt
    13681 ttgccattgg ctaaggagac aatcggctga tagtgcacgc aaatatcacg attttcgata
    13741 gcatcctgca gccgatgatg cggtgactgg atacggcgca aaatacgcag cacaaacatc
    13801 gctgccagca ggccaatcac caacccgagc ggtaaccaaa taaagacttg ccgatgccag
    13861 cctttctgca acattttcgt tgaagcccag gtgatgatcg aaatattcat ctccgggaag
    13921 ggcaggatat cgtaaatgat tccattattt tcgatatgct caccgggtgt tttttgtagc
    13981 ctggtaataa ttccctgagc aatttcatcg ctgctggtta tgacaacgtt atgggcattg
    14041 ccaataatgg cggcatcaat ttgccatgag ctatagggaa tgacatcaat aaaggaagcg
    14101 gggtcgatca tgacgacata atgtgccgtt cccatggcga ccatgtaacg gataatgcct
    14161 aaatcgttat gcgatgttaa ccagacacga taaccatctt tcgaaatttt acctggctcg
    14221 gggaaggtat cgggcggact ttcatgctcc agagacgaac actggggaac gttgttatcg
    14281 atataaacca cttcctgaat atagcgataa ctgtaagata cccgacgcat ttccatgaga
    14341 tgggcttcgc tacaggcagc gccttgccat ctttccagct cttgcagcgc atctttccct
    14401 tgtgtcgcca ccttattggc ccgaatagcg acgcgggagg aataggtatc cagctcttca
    14461 ataaacgatg tttctacctg ctgatgggcc agccagatgc ttaagccgac aggcagcaat
    14521 actgaaagaa tcagtactcc cgaaataagg ccgaccagat gtcgtgttct caccagcata
    14581 tccttattaa cacagtgtag ggaaagtata tctgattgtg gaaaaaggct ggcaggagat
    14641 aaggaggtag agctggtatt tcatgaagtg atgggtggaa acgatcattg tcatttgacc
    14701 aagcgcagag ctttacgcct ggtatgtaac cctaacttaa gatgttgatt cggcgcgagc
    14761 gagggtaaaa atatcgtaaa aagacagttc acctttacgg acgatcattt tttgcagttg
    14821 ttccttatga acatccgcga cacgcggaga gttcaagatg gcttcaacca gcgcggcagg
    14881 tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca gcacgtcgat
    14941 atcttcatag cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa gcaggataaa
    15001 taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca tcaccgccag
    15061 ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac ggataaaact
    15121 gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact caatcgtttc
    15181 ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca actttccagg
    15241 gaacacccta ttttaacgtg ggaatagtag aggggaagta acagatttac cacaattttg
    15301 cataacagtt gcgaaaaacc gattacaatt ttttaattat tctggctgga ttaccgccca
    15361 cgacaacgtt gtccgggaca tcttttgtga caactgcacc tgaggctacc acgacgttat
    15421 caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg ttattaccga
    15481 tggtgacggg tttccccagt tcagcaccgc tattacgtgc tacagggtcg atgggatgtg
    15541 ttgccgtgta gatatgaacg cctggtgcca acatacagtt atcaccgatg cgaatagggc
    15601 agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attaccgaga aaaatgttat
    15661 agccatagtc acagcgaaac gttggctcaa tataagcctc tgtcacctga ccgaatagat
    15721 cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg tatcggtgaa
    15781 taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag cgatacaact
    15841 caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg ctgaaaatgc
    15901 ggcaacacag taagacgaaa agtcagtgaa gagaacgttc ccattacatc tgtgtgataa
    15961 agatcacata gggtatgtct tcatggtgaa aagtataaaa ttcttaataa acagccggtt
    16021 atagctccga aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt
    16081 gtcgtatagt ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac
    16141 cgccagttca ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc
    16201 aattgtctgg caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga
    16261 catacttcta cccatggttg taataattct aacaagataa actcgcagga ttctctttcg
    16321 tcgcattgac aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct
    16381 gcatattaat accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct
    16441 gctcaatgag cttattgtct tcattatact taattttgta attaagtgcg aaggtctcaa
    16501 tatgctcaat cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc
    16561 catgattgct ttcttcaagg tttgcaaggc agtcatgata cagggtttca cagagaaact
    16621 taagctgtgc gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta
    16681 gcggttgaac taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct
    16741 ctcgtttgtt agcttaacta taagccactc tttgcaggtt ttcatcgcat tattacgaaa
    16801 aattacaatt aggaaggaat cgggacagga ctcacttttt catactagca ctaacccgca
    16861 gcaggtaaaa gcagtctgct cgaactgttc ataacatttc aggattatcg ccagatgaaa
    16921 ttggtgacac taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa
    16981 gaattaccgc aaataattaa gaatagcctc taaatgatta tggattatta aatctattag
    17041 cgctacttaa tataaattaa ctaacaatca gtatttttta tgaattttat ccgtggttaa
    17101 tactggtttt cgtatgagat cctgagttgg tggttcaatt actccttaat gttcgtaggt
    17161 tatgcataaa aaaggccgct tgcgcggcct tagtgattac acgttgtatc aatgatgatc
    17221 gacagtatgg ctgtgctcga tatcttcatt cttgcggcta aagcggcggc gaaccaccac
    17281 aaagaatacc ggaacgaaga agattgccag taccgttgcg gtcaccatcc cgcccattac
    17341 accggtacct actgcgttct gcgcgccgga accagcacca gtactgataa ccagcggcat
    17401 aacgccgagg ataaacgcca gcgaggtcat caggatcgga cgtaaacgca tccgcactgc
    17461 atcaagcgtc gcttcaatca gacctttacc ttctttatcc atcaagtctt tggcgaattc
    17521 gacgataagt atcgcgttct tcgccgacaa cccaatggtt gtgagcaggc ctacctggaa
    17581 gtaaacgtca ttggtcaggc cacggaaggt ggcagccagc aacgcaccga taacccccag
    17641 cggaacgacc agcataacgg agaacggaat cgaccagctc tcgtacagcg ccgccagaca
    17701 caggaatacg acaatcaacg aaatcgcgta cagtgaaggt gcctggttgc cggagagacg
    17761 ttcctgatag gacatccccg tccagtcata gccaacaccg gtaggcagtt tgctcgccag
    17821 ttgttccatc agctccattg cttcaccggt acttttaccc ggtgccgcct ggcctaagat
    17881 ttccatggat ggcaggccgt tgtaacgttc cagacgcggc gaaccgtact cccaacgaga
    17941 agaggagaac gccgagaatg gcaccatctg accatcagca gcacgaacat accagtcgcc
    18001 gatatcatcc ggcagcatac ggtatttcgc ttctgacatg acgtaaactt tcttcacacg
    18061 accgcggtcg ataaagtcgt tcacatagct accgccccat gcagcgccca gagaggtgtt
    18121 aatgtcgttg atagaaacac ccagcgcctg cgctttttcc tggtcgatat caatcttaaa
    18181 ctgcggggta tcttccagac cgtttggacg tacgctggtc agcatatcag ggtgcttcgc
    18241 tgcttctgca agcagctggt tacgcgcctg agtcagtttt tcgtgaccaa ggccagcctg
    18301 gtcaatcagc tcaaagtcaa agccggttgc agtacccagt tccacgattg cgggcaggtt
    18361 aaaggcgaaa accatcgcat ctttgatttg cgagaaagcg cgtgttgcac gcatggtaat
    18421 cgcttcaact ttgttttctt cgcccggacg atcggcccag tccttcaagg aaacgaacgc
    18481 aataccggta ttctgaccac gtcccgcaaa gccgaagccg ttaacggcga acaccgactc
    18541 aacgttgttc ttttctttgg tcagatagta atgcgttacc tcattgagca ctttctgcgt
    18601 acgttcctgc gttgcacctg ctggcagctg aaccatggtc ataaatacgc cctggtcctc
    18661 atctggcaag aaggagcttg gcagacgcac gaacagatag gccatgccga ccacgatgat
    18721 cagatacagc accaggtaac gccccgtact gcgcagaata ccgcctacgc tgtcggtgta
    18781 gtggtgcgtg ctcttctcga acatgcggtt aaaccagccg aagaagcctt ttttaccttc
    18841 cccgtgatcg cctttggcaa tcggtttcag catggtggca caaagagccg gagtcaggat
    18901 caacgctacc agtaccgaca gcgccattgc tgaaacaatg gtaatagaga actgacgata
    18961 gatagcacca gtagaaccgc caaagaaggc catcggtacg aataccgccg acagtaccat
    19021 cgcgataccg accagagcgc cctgaatctg ccccatcgac ttacgggtag cttcttttgg
    19081 cggcaaacct tcttccgcca taacacgctc cacgttttct accacaacga tagcgtcatc
    19141 caccaacaag ccgatggcga gcaccatccc gaacattgtt agcgtgttta tcgagaagcc
    19201 aaaggcggca aggacggcaa aggtcccgag caataccacc ggtacggcaa tggtcggaat
    19261 caacgtcgcg cggaagttct gcaggaacag atacataacc aggaacacga ggatgatcgc
    19321 ttcgaccagc gttttaacca cttcgtgaat agagattttc acgaacggcg tggtgtcgta
    19381 cgggtaaaca attttcagac ccgacgggaa gaacggttcc atcttcgcca gttcagcacg
    19441 gattgccgca gcggtatcca gcgcgtttgc accggtcgcc agcttgatcc ccagaccgga
    19501 agccggttgg ccgttaaact ctgcgatgat gtcgtagttc tcaccaccca gctcaatctt
    19561 cgccacatca cgcagcagta cgcgggaacc atcctgattc actttcagca ggattttgcc
    19621 gaactcttca gtagaggtca gacgcgtctg agcaataata gaggcgttaa gctgttggcc
    19681 tttcaccggc ggcgtaccac cgagctgacc cgccgcaacc tgggcgttct gcgctttgat
    19741 ggcggtaatg acatcaaccg gcgttagctg gaatttgttc agctcattcg ggttcatcca
    19801 gatacgcatc gcgtactgtg aaccgaacaa ctgaacatca cccacgcccg acgtacggct
    19861 gatggcatct ttcatattcg ccgctacgta gtcagagata tcctcctgcg tcatggtgcc
    19921 atcggtgttg ataacgccga caaccatcag gaagctgctg gatgatttct caacgctcac
    19981 cccttgctgc tgaacttctt gcggcagcaa cggcatcgcc agctgcagtt tgttctgcac
    20041 ctgaacctgc gcgatatccg catcagtacc agactcaaag gtcagggtga tctgcacggt
    20101 acccgtggag tcactgttag aggacatgta catcaggtta tcgataccgt tcatattctg
    20161 ttcgataacc tgtgtcaccg tgtcctgcac tgttttcgca tcagcgccgg ggtaggaggc
    20221 ggagatcgtt actgccggcg gtgcaatcgt aggatattgc gccaccggca gtttgaggat
    20281 cgccagcccc cctgccaaca tgatgataat ggcgatcacc cacgcaaaaa tcgggcgatc
    20341 gataaagaaa ttaggcatgt cttaacggct cctgtttaag ttaagacttg gactgttcag
    20401 gctgagcacc gcttgcggct tgctggttat tatcagcggt aacttcttgt gcttttacct
    20461 ggacaccagg acgcactttc tgcagcccac ttattactac gcgatcgcct gctttcagac
    20521 cttctgtcac cagccactta tcgccaatag cctggcttgc aacgatcgga cgggtttcca
    20581 ctttgtcatc cgcgccaact accagtacgg tggcatcacc acgcggcgta cgggttacgc
    20641 cctgttgcgg gactaaaata gcgtttggat taagcccttc ttccagacgt gcgcgcacga
    20701 acatacccgg cagcagagtg tgatccgggt tcgggaagat agcgcgtagg gtgatagacc
    20761 cagtggtctg atcaacggta acgtcagaga attccagcgt accgtcctgc gggaacttaa
    20821 tgccgtcact ggtgatcagc gacactttgg ctttgccgtt ctcttgtttc agcgtgccat
    20881 tcgccagttc ctgtttcagg cgcaggaagt cgttgctgga ctgggtcaca tcaaggtaga
    20941 tcggatcaag ttgctgcacg gttgccagcg cagtcgcctg accgttctgt accaatgcgc
    21001 cttccgtcac gttcgactta ccaatgcgac cgctaatcgg agaggtgact ttggtgtaag
    21061 ccagattgat ccgcgcagtt tcaacggcag ctttcgccgc agttaccgca gcattcgcct
    21121 gttgcgcatc agccagagcc tgatcgtact cttgcttact gatgtactga gtaccgagca
    21181 gtttctgata acgattcacc gtcaattgcg cgatattggc tgcagcctgg gctttcgcca
    21241 gatcaccttt cgcactgtcg tatgtcgcct gataggtcgc aggatcaatc tgatagagag
    21301 agacaccagc ttcgatgtcg ctaccttctt tgaaattacg cttcaggata atcccgctaa
    21361 cttgaggacg aacttctgcg atccggtagg cactggtgcg acccggaagc tcggttgtga
    21421 tctgcagagg ttcagttttg actgttacta cgccaacggc gggcatctgc tggccacctt
    21481 gttgggcctg tttgtcgtca catcctgtta gggctaagct gcctgagagc atcagaacga
    21541 ccgccagagg cgtaaaccct ctgtttttgt tcatatgtaa acctcgagtg tccgatttca
    21601 aattggtcaa tggtcaaaag ttaataaacc cattgctgcg tttatattat cgtcgtgcta
    21661 tggtacatac attcacaaat gtatgtaaat ctaacgcctg taaattcacg aacatatggc
    21721 acgaaaaacc aaacaagaag cgcaagaaac gcgccaacac atcctcgatg tggctctacg
    21781 tcttttctca cagcaggggg tatcatccac ctcgctgggc gagattgcaa aagcagctgg
    21841 cgttacgcgc ggtgcaatct actggcattt taaagacaag tcggatttgt tcagtgagat
    21901 ctgggaactg tcagaatcca atattggtga actagagctt gagtatcagg caaaattccc
    21961 tggcgatcca ctctcagtat taagagagat attaattcat gttcttgaat ccacggtgac
    22021 agaagaacga cgtcgattat tgatggagat tatattccac aaatgcgaat ttgtcggaga
    22081 aatggctgtt gtgcaacagg cacaacgtaa tctctgtctg gaaagttatg accgtataga
    22141 acaaacgtta aaacattgta ttgaagcgaa aatgttgcct gcggatttaa tgacgcgtcg
    22201 ggcagcaatt attatgcgcg gctatatttc cggcctgatg gaaaactggc tctttgcccc
    22261 gcaatctttt gatcttaaaa aagaagcccg cgattacgtc gccatcttac tggagatgta
    22321 tctcctgtgc cccacgcttc gtaatcctgc cactaacgaa taaccctgaa tctgactcca
    22381 ggatttttcc tggacatttt cgtcgttgct attctggttc actgcgtcgt gatattcttg
    22441 cggtttgact ttttcaggtc gttcttcagg ttcagaaacc ttcattcatc atgactatgt
    22501 tccagtatta caaacgatca cggcattttg ttttttcagc atttattgct tttgtttttg
    22561 tcttgttatg ccagaacacg gcgtttgcgc gggcgtcatc gaatggtgat ctgccgacaa
    22621 aagcggacct gcaggcgcaa cttgactcac taaataaaca aaaagatctt tctgctcagg
    22681 acaaactggt gcagcaggat ctgacagata cattagccac cctcgataaa atcgatcgca
    22741 taaaagaaga gacagttcag ctacggcaaa aagtcgctga agcgccagaa aaaatgcgcc
    22801 aggcgaccgc ggcgttaaca gcacttagcg atgtcgataa cgacgaagaa acgcgcaaaa
    22861 ttctgagtac gctttcgttg cgccagctgg aaactcgcgt tgcccaggcg ctggacgatt
    22921 tgcaaaacgc acaaaacgat ctggcgtctt ataacagcca gctggtttcg ttacagacgc
    22981 agcccgaacg cgtgcaaaat gcgatgtata acgcttcgca gcagctgcaa caaattcgca
    23041 gtcgtctgga tgggactgat gtcggcgaga ctgccttacg tcccagccag aaagtgttaa
    23101 tgcaggctca gcaggcgttg ctgaatgcgg agattgacca gcagcgtaaa agcctggaag
    23161 ggaacaccgt cttgcaggat accttgcaaa agcaacgtga ttacgtgacg gcgaacagcg
    23221 cccgtctgga gcaccagttg cagctgttgc aagaagcggt aaacagcaag cgcctgactt
    23281 taactgaaaa aacggcgcag gaagccgtct ccccggatga agccgcgcgt attcaggcta
    23341 atccgctggt gaaacaggaa ctggaaatta accagcagtt aagtcagcgt ctgattaccg
    23401 cgactgaaaa cggtaatcag ttgatgcagc aaaacattaa agtcaaaaac tggctggagc
    23461 gggcgctgca atcggaacgc aatattaaag agcagattgc cgtcctgaag ggcagcctgc
    23521 tgttgtctcg tatcctttac cagcaacaac aaacgctgcc ctcggcggat gaactggaaa
    23581 acatgaccaa ccgcatcgcg gatttgcgtc tcgaacagtt tgaagttaac cagcagcgtg
    23641 atgcactctt ccagagcgat gcgttcgtca acaaactgga agaaggtcac accaacgaag
    23701 tcaacagcga agttcacgat gcgttattgc aagtggttga tatgcgtcgc gaattgctgg
    23761 atcaactcaa caaacagttg ggtaaccagc tgatgatggc cattaacctg caaatcaacc
    23821 agcagcagtt aatgagcgta tcgaaaaacc tgaaatccat cctgactcag caaatctttt
    23881 gggtgaacag taaccgtcca atggactggg actggatcaa agcgttcccg caaagcctga
    23941 aagatgaatt taagtcgatg aaaatcacgg tgaactggga aaaagcctgg cctgccgttt
    24001 ttatcgcttt cctcgccggt ttgccgctgc tgttgattgc cgggctgatc cactggcgtc
    24061 tgggctggct gaaagcgtat caacagaaac tggcttccgc tgtgggttcc ctgcgtaacg
    24121 atagccagct caacacacca aaagcgatcc ttatcgacct gatccgcgcg ctgccggtgt
    24181 gcctgattat tctcgcggtt ggcctgattc tgttgaccat gcagctcaac atcagcgaac
    24241 tgctatggtc gttcagtaaa aaactggcga ttttctggct ggtgtttggc ctgtgctgga
    24301 aggtactgga gaaaaatggc gttgccgtgc gtcacttcgg catgccggag cagcagacca
    24361 gccactggcg tcggcaaatt gtccgcatca gtctcgcgtt gctgcctatc catttctggt
    24421 ctgtggtggc agaactttcc ccgctgcatc tgatggatga tgtgctgggg caggcgatga
    24481 ttttcttcaa cctgctgctg attgccttcc tggtatggcc aatgtgccgc gaaagctggc
    24541 gtgataaaga gtcgcacacc atgcgactgg tcaccattac cgtgctgtcg attatcccga
    24601 ttgcattgat ggtgctgact gccacgggtt atttctacac cacgctgcgc ctctccgggc
    24661 gttggattga aaccgtttat ctggtgatca tctggaactt gctctaccag acggtattgc
    24721 gcggcttaag cgtagcggcg cggcgtatcg cctggcgtcg tgcgctggcg cgtcggcaga
    24781 atctggtgaa agagggcgca gaaggtgccg aaccgccgga agaacccacc attgcactgg
    24841 aacaggttaa ccagcagacg ctgcgtatta ccatgttgct gatgtttgcg ctgttcggtg
    24901 tcatgttctg ggcaatttgg tccgatttga tcaccgtgtt cagctatctc gacagcatca
    24961 cgctctggca ttacaacggc actgaagctg gcgctgcggt ggtgaaaaac gtcaccatgg
    25021 gcagtctgtt gtttgcgatt atcgcctcaa tggtggcctg ggcgttgatt cgcaacctgc
    25081 ctggtttact ggaagtgctg gtgctctcgc gactgaatat gcgtcagggc gcgtcgtatg
    25141 ccattactac catccttaac tacatcatta ttgccgttgg tgcgatgacg gtgttcggat
    25201 cgctgggcgt ctcttgggat aaactccagt ggctggccgc agcattatcc gtaggtcttg
    25261 gttttggttt acaagaaatt ttcggtaact tcgtctccgg tttgatcatt ctgttcgaac
    25321 gtccggtgcg tattggcgat acggtaacca ttggtagctt ctcggggacg gtaagtaaga
    25381 tccgtattcg tgcgacaacg attaccgatt tcgatcgcaa agaagtgatc atcccgaaca
    25441 aagcgtttgt taccgagcgt ctgatcaact ggtcgttgac tgacactact acgcgtctgg
    25501 tgatccgtct cggcgtggcc tatggctccg atctggaaaa agtgcgtaaa gtgttactga
    25561 aggcggcgac tgagcaccca agggtgatgc acgaaccaat gccggaagtc ttctttacgg
    25621 cgtttggtgc cagcacgttg gatcatgagc tgcgtctgta tgtgcgtgaa ctgcgtgacc
    25681 gtagtcgtac tgtcgatgag ctgaaccgta ctatcgatca gctgtgccgt gaaaacgaca
    25741 tcaacattgc ctttaaccag cttgaagtgc atctgcacaa cgagaagggc gatgaggtga
    25801 cggaagtcaa acgcgactac aaaggcgatg acccgacgcc agcggtaggg taataaccaa
    25861 agggggcgac atttagttgc cccgagatta ctgacaaagt gcgcgttgtt catgccggat
    25921 gcggcgtgac cgccttatcc ggcctacgaa accgcaagaa ttcaatgtat tgcaggagcg
    25981 gtgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgcccaca atcttagggg
    26041 acatttagcg gccccattta tttctcactt ttccgcctta tcatcgcgcg ttaatttctt
    26101 ttcataatcg cgttttacaa tatccagcgc gcgcagcacg gtgcgggctg ggatctgatt
    26161 ttcttccagc agcacaatca aatcgacagc cagtttgaca tcgtcagggg cattttcaag
    26221 tgacatactc tctccattgc tagcgggtta agcgcgctag cctgttttcg attttttcca
    26281 gcgcatggcg gcagcgtgcc aggcgcgctt cataggcttc cacttcacga tgcagcgttt
    26341 gctgttccac gagatcggtc acccgcgcca ggcgggctct gcgttcggca accatctcac
    26401 gtagccgccg ctcaaaatcc tgatgctgaa tacgtttacg ctgccagcgg gcaattttcg
    26461 gtggcgcact gtcccactcg cgcaatgacc aggcggaggc ttcacgcgcg atggcttcca
    26521 gttgtgccgc cagatgttcc gccagccagg ccacttgcgg cagctgttgc tgctcaaccg
    26581 catgacaaag cgcagccaga ttatcgcccg cctcgtcgag acaagcctgt agtgttgtcg
    26641 cacgagtctg aaaaagatgc ctgtcgaaac gagcacttag cgtggcgaac tgtgacaccg
    26701 gggcacaacg ctgacgcagc gtagcgagct gtccttccag tttttccagc agcagggcgg
    26761 ttttcacgat aagacctcaa tgaaaatgat aattgttatg ctaaagtagc cactctgtaa
    26821 gctgacaaca ctcaatatgc aacgaatcat tttaatcatc attggctggc tggcggtagt
    26881 gctgggtacg ctgggcgtgg tattaccggt attaccgacg acgccgttta tcctgctggc
    26941 ggcctggtgc tttgcccgtt cttccccgcg ctttcacgcc tggttgctgt accgctcatg
    27001 gtttggcagc tatctacgtt tctggcagaa acatcatgcg atgccgcgcg gcgtcaaacc
    27061 gcgggcgatt ttgcttattt tgctcacgtt tgccatttct ctgtggttcg tccagatgcc
    27121 atgggtgcgc atcatgttgc tggtaattct cgcctgtttg cttttctata tgtggcgaat
    27181 tccggtgatt gatgaaaagc aagaaaagca ctgaagcaca acaatcgcag ttgcaattat
    27241 tgcgtacagc cagtacattc tggcgttttc gagcacaggc gcaggcggtc aaaggttaaa
    27301 caactgttac ttttgatacg tttaaaacgc gccgtgagta ccaccgtaac aagcaggcat
    27361 acacttatga ccgcgactgc acagcagctt gagtatctca aaaatagcat caaaagcatt
    27421 caggactacc caaaacccgg cattcttttc cgcgatgtca ccagcttact ggaagacccg
    27481 aaagcttacg ctctcagcat cgacttgctg gttgagcgtt acaaaaatgc gggcattacc
    27541 aaagttgtcg gcaccgaagc gcgtggcttc ttgtttggcg ctccggtagc tctgggtctg
    27601 ggcgttggct ttgtaccggt ccgtaaaccg ggcaaactgc cgcgtgaaac catcagtgaa
    27661 acttacgacc tggaatacgg caccgatcag ctggagatcc acgttgatgc catcaaaccg
    27721 ggcgacaaag ttctggtggt ggacgatctg ctggcaaccg gcggcactat cgaagcgacc
    27781 gttaaactga tccgtcgtct gggtggtgaa gtggctgacg ctgcgttcat tatcaacctg
    27841 ttcgatctcg gcggcgaaca gcgtctcgaa aaacagggca ttaccagcta cagccttgtc
    27901 ccgttcccgg gccattaatt atcgccagtc ttgtgctgcc cacgctacgg acagcacaag
    27961 atgtgcattc agcctcgccg ttctgacggg gctgtgttag cattacccct tcgtgaatcc
    28021 accttccagc gtttcagagc ctgccaatga gttatcaggt cttagcccga aaatggcgcc
    28081 cacaaacctt tgctgacgtc gtcggccagg aacatgtgct gaccgcactg gcgaacggct
    28141 tgtcgttagg gcgtattcat catgcttatc ttttttccgg cacccgtggc gtcggaaaaa
    28201 cctctatcgc ccgactgctg gcgaaggggc taaactgcga aaccggcatt accgcgacgc
    28261 cgtgcggcgt gtgcgataac tgtcgtgaaa tcgagcaggg gcgctttgtc gatctgattg
    28321 aaatcgacgc cgcctcgcgc accaaagttg aagatacccg cgacctgctg gataacgtcc
    28381 agtacgctcc ggcgcgtggt cgtttcaaag tttatctgat cgacgaagtg catatgctgt
    28441 cgcgccacag ctttaacgca ctgttaaaaa cccttgaaga gccgccggag cacgttaagt
    28501 ttctgctggc gacgaccgat ccacagaaat tgccggtgac gattttgtcg cgctgtttgc
    28561 aatttcatct caaggcgctg gatgtcgagc aaattcgcca tcaacttgag cacatcctca
    28621 acgaagaaca tatcgctcac gagccgcggg cgttgcaatt gctggcgcgc gccgctgaag
    28681 gcagcctgcg agatgcctta agtctgaccg accaggcgat tgccagcggt gacggccagg
    28741 tttcaaccca ggcagtcagt gcgatgctgg gtacgcttga tgacgatcag gcgctgtcgc
    28801 tggttgaagc gatggtcgag gccaacggcg agcgcgtaat ggcgctgatt aatgaagccg
    28861 ctgcccgtgg tatcgagtgg gaagcgttgc tggtggaaat gctcggcctg ttgcatcgta
    28921 ttgcgatggt acaactttcg cctgctgcac ttggcaacga catggccgcc attgagctgc
    28981 ggatgcgtga actggcgcgc accataccgc cgacggatat tcagctttac tatcagacgc
    29041 tgttgattgg tcgcaaagaa ttaccgtatg cgccggaccg ccgcatgggc gttgagatga
    29101 cgctgctgcg cgcgctggcg ttccatccgc gtatgccgct gcctgagcca gaagtgccac
    29161 gccagtcctt tgcacccgtc gtgccaacgg cagtaatgac gccaacccag gtgccgccgc
    29221 aatcagcgcc gcagcaggca ccgactgtac cgctcccgga aaccaccagc caggtgctgg
    29281 cggcgcgcca gcagttgcag tgcgtgcagg gagcaaccaa agcaaaaaag agtgaatcgg
    29341 cagccgctac ccgcgcgcgg ccggtgaata acgctgcgct ggaaagactg gcttcggtca
    29401 ccgatcgcgt tcaggcgcgt ccggtgccat cggcgctgga aaaagcgtca gccaaaaaag
    29461 aagcgtatcg ctggaaggcg accactccgg tgatgcagca aaaagaagtg gtcgccacgc
    29521 cgaaggcgct gaaaaaagcg ctggaacatg aaaaaacgcc ggaactggcg gcgaagctag
    29581 cggcagaagc cattgagcgc gacccgtggg cggcacaggt gagccaactt tcgctaccaa
    29641 aactggtcga acaggtggcg ttaaatgcct ggaaagagga gagcgacaac gcagtatgtc
    29701 tgcatttgcg ctcctctcag cggcatttga acaaccgcgg tgcacagcaa aaactggctg
    29761 aagcgttgag catgttaaaa ggttcaacgg ttgaactgac tatcgttgaa gatgataatc
    29821 ccgcggtgcg tacgccgctg gagtggcgtc aggcgatata cgaagaaaaa cttgcgcagg
    29881 cgcgcgagtc cattattgcg gataataata ttcagaccct gcgtcggttt ttcgatgcgg
    29941 agctggatga agaaagtatc cgccccattt gatcgtaagc acagcttacg ttcgtcatcc
    30001 ttaacgtgat tgagagagaa acctatgttt ggtaaaggcg gtctgggtaa cctgatgaag
    30061 caagcccagc agatgcaaga aaaaatgcag aaaatgcagg aagagatcgc gcagctggaa
    30121 gtcaccggcg aatctggcgc aggtctggta aaagtgacca tcaacggtgc acacaactgc
    30181 cgtcgcgtag agatcgaccc gagcctgctg gaagacgaca aagagatgct ggaagacctg
    30241 gtggctgcag cattcaacga cgcagcacgt cgtattgaag aaacgcagaa agaaaaaatg
    30301 gcctctgtat cctccggaat gcagctgccg cctggcttta agatgccgtt ctgatgcaaa
    30361 ccagcccgct gttaacacag cttatggaag cactgcgctg tctgccgggc gttggcccga
    30421 agtcggcgca gcgtatggcg ttcacgctgc ttcagcgcga tcgtagcggc gggatgcgtt
    30481 tggcgcaggc gctcacccgg gcgatgtcgg aaatcggcca ctgcgccgat tgccgcactt
    30541 tcaccgaaca ggaagtctgt aacatctgtt cgaatccgcg tcgtcaggaa aacggtcaaa
    30601 tctgtgtggt ggagagtccg gcggacatct acgccattga gcagacgggg cagttttctg
    30661 gtcgttattt tgtgttgatg gggcatctgt caccgctgga cggcatcggt ccggatgata
    30721 tcgggcttga tcgtctggaa cagcgtctgg cagaggaaaa aatcactgaa gtgatcctcg
    30781 ccaccaatcc cacggttgaa ggtgaagcta ccgctaacta cattgccgag ctttgcgcgc
    30841 aatatgacgt ggaagccagc cgaatcgctc atggcgtacc ggttggcggc gaactggaaa
    30901 tggtcgatgg caccacgctg tcacactccc ttgccgggcg tcataagatt cgtttttaag
    30961 caaacgagag caggatcacc tgctctcgct tgaaattatt ctcccttgtc cccatctctc
    31021 cctcatcctg tttttaacct taaaatggca ttattgaggt agacctacat gaaaggacaa
    31081 gaaactcgtg gttttcagtc agaagtgaaa cagcttctgc acctgatgat ccattctctc
    31141 tattccaata aagaaatctt cctgcgtgag cttatctcta acgcctccga tgcggcggac
    31201 aagctgcgtt tccgtgcgct atctaacccg gacctgtacg aaggtgatgg cgaactgcgc
    31261 gttcgtgtct ctttcgataa agacaagcgc acgctgacca tctctgataa cggcgtgggg
    31321 atgacccgcg acgaagtgat tgaccatctg gggactatcg ctaaatccgg taccaaatca
    31381 ttcctcgaat ccctgggttc tgaccaggcg aaagacagcc agctgatcgg tcagtttggt
    31441 gttggtttct actctgcgtt tatcgtggcc gacaaagtga ccgtgcgtac tcgcgcggca
    31501 ggcgaaaaac cagaaaacgg cgtcttctgg gaatcggctg gcgaaggtga atataccgtt
    31561 gccgacatca ccaaagaaga tcgtggtact gaaatcaccc tgcatctgcg tgaaggcgaa
    31621 gacgagttcc tcgatgactg gcgcgtgcgt tccatcatca gcaaatactc cgaccatatc
    31681 gcgctgccgg tagagatcga aaaacgcgaa gagaaagacg gcgaaaccgt tatctcctgg
    31741 gagaaaatca acaaagcgca ggcgctgtgg actcgtaaca agtcggaaat caccgatgaa
    31801 gagtacaaag agttctacaa acacatcgcc cacgacttta atgatccgct gacctggagc
    31861 cacaaccgcg ttgaaggtaa gcaggagtac accagcctgc tgtacattcc gtcccaggct
    31921 ccgtgggata tgtggaaccg tgatcataaa cacggcctga aactgtacgt ccagcgtgtg
    31981 ttcatcatgg acgacgcaga acagttcatg ccgaactatc tgcgcttcgt gcgtggtctg
    32041 attgactcca gcgatctgcc gctgaacgtt tcccgtgaaa tcctccagga cagcacggta
    32101 acgcgtaacc tgcgcaatgc gctgaccaag cgtgtgctgc aaatgctgga aaaactggcg
    32161 aaagacgacg cggaaaaata ccagaccttc tggcaacagt ttggcctggt actgaaagaa
    32221 ggtccggcgg aagatttcgc taaccaggaa gcgatcgcca aactgctgcg ttttgcttct
    32281 acccataccg attcttctgc gcagaccgta tctctggaag actacgtttc ccgcatgaaa
    32341 gaagggcagg agaaaatcta ctacatcacc gcagacagct atgcggcagc gaagagcagc
    32401 ccgcacctgg aactgctgcg taagaaaggc atcgaagttc tgctgctttc cgaccgcatc
    32461 gatgagtgga tgatgaacta tctgactgag ttcgacggta aaccgttcca gtcggtgtct
    32521 aaagttgacg agtcgctgga aaaactggct gacgaagttg atgagagcgc gaaagaagcg
    32581 gagaaagcac tgactccgtt catcgaccgt gtgaaagccc tgctcggcga gcgcgtgaaa
    32641 gatgtccgtc tgactcaccg tctgaccgat acgccagcga tcgtttcgac cgacgcggac
    32701 gaaatgagca ctcagatggc gaaactgttc gctgcggcgg gccagaaagt gccagaagtg
    32761 aaatacatct tcgaactgaa cccggatcac gtactggtga aacgtgcggc agatactgaa
    32821 gatgaagcga agttcagcga gtgggtagaa ctgctgctgg atcaggcgct gctggcagaa
    32881 cgcggcacgc tggaagatcc gaacctgttt attcgtcgta tgaaccagct gctggtttcc
    32941 tgatgtcatg ccggatgacc tttgtgtcat ccggcatttt tcttttcatc atctgcactt
    33001 tccgcaaatt atctcgccat taaccgtttc agcctcaggt gcctttcttg aggcaatcgc
    33061 ctgttggtgg tatcgtttat cgctttttca aaaaaatcga cacattttaa ggggattttc
    33121 gcaatgcgta tcattctgct tggcgctccg ggcgcgggga aagggactca ggctcagttc
    33181 atcatggaga aatatggtat tccgcaaatc tccactggcg atatgctgcg tgctgcggtc
    33241 aaatctggct ccgagctggg taaacaagca aaagacatta tggatgctgg caaactggtc
    33301 accgacgaac tggtgatcgc gctggttaaa gagcgcattg ctcaggaaga ctgccgtaat
    33361 ggtttcctgt tggacggctt cccgcgtacc attccgcagg cagacgcgat gaaagaagcg
    33421 ggcatcaatg ttgattacgt tctggaattc gacgtaccgg acgaactgat tgttgatcgt
    33481 atcgtaggcc gccgcgttca tgcgccgtct ggtcgtgttt atcacgttaa attcaatccg
    33541 ccgaaagtag aaggcaaaga cgacgttacc ggtgaagaac tgactacccg taaagacgat
    33601 caggaagaaa ccgtgcgtaa acgtctggtt gaataccatc agatgactgc accgctgatc
    33661 ggctactact tcaaagaagc ggaagcgggt aacaccaaat acgcgaaagt tgacggcacc
    33721 aagtcggttg ctgaagttcg cgctgatctg gaaaaaatcc tcggctaatc aagccgcagc
    33781 agatctgctt tcgggcaggt ctgctcatcc acctcaggca atacctctca cagcaattag
    33841 ttcttcttcc tcacttttcc gctacaatta tcaacaagtt gaatcgataa gaggcggtaa
    33901 tgcgtcagac taaaatcggt atcctgctgg caaacctggg tacgcccgat gcccccacac
    33961 ctgaagcggt aaaacgctat ctgaaacaat ttttaagcga cagacgcgtg gttgatacct
    34021 cacggttgtt atggtggcca ttgctgcgcg gcgttatttt gccgctgcgc tcgccgcgtg
    34081 tggcgaagct gtacgcctcg gtctggatgg aaggtggctc gccgctgatg gtttacagcc
    34141 gccagcaaca gcaggcgctg gcacaacgtt taccggagac gcccgtagcg ctgggaatga
    34201 gctacggctc gccatcactg gaaagcgccg tcgatgaact cctggcagag catgtagatc
    34261 atattgtggt gctgccgctt tatccgcaat tctcctgttc tacggtcggt gcggtatggg
    34321 atgaactggc acgcattctg gcgcgcaaac gtagcattcc ggggatatcg tttattcgtg
    34381 attacgccga taaccacgat tacattaatg cactggcgaa cagcgtacgc gcttcttttg
    34441 ccaaacatgg cgaaccggat ctgctgctgc tctcttatca tggcattccc cagcgttatg
    34501 cagatgaagg cgatgattac ccgcaacgtt gccgcacaac gactcgcgaa ctggcttccg
    34561 cactggggat ggcaccggaa aaagtgatga tgacctttca gtcgcgcttt ggccgggaac
    34621 cctggctgat gccttatacc gacgaaacgc tgaaaatgct cggagaaaaa ggcgtaggtc
    34681 atatacaggt gatgtgcccg ggctttgctg cggattgtct ggagacgctg gaagagattg
    34741 ccgagcaaaa ccgtgaggtc ttcctcggtg cgggcgggaa aaaatatgaa tatattccag
    34801 cgcttaatgc cacgccggaa catatcgaaa tgatggctaa tcttgttgcc gcgtatcgct
    34861 aaagctgagc ggtaaagaac tgagcgccgt cgcgaagagc ctcgtcggcg gttttcatca
    34921 tccgtgaata atgcaaaaag gcatgcagcg tacctgggta gagtttgaac tcacagggct
    34981 gctgatgcgc cgctaacgtc tggtaaagca gacggctgtc atccagcagc ggatcgaact
    35041 ccgccccggc aataaaacag ggcggaactt cgcgagtgag atcattatta aacagacagt
    35101 aatacggtga ctcgcggtcc gcgtcgttgc ttaaatacgc ctcttcatac atctgcaaat
    35161 cctgttgcgt taagccatcc cagacaccgc ccaacagacg acgagtcacg gaatcccgta
    35221 atccgtaaag cccataccac agcaaaacac ccgcaatttt accgcaatcg atctgtttat
    35281 cacgcaacca caacgcactg gcgagcgcca gcatggcacc tgcggaatca ccggcaaagc
    35341 caatgcggga catattgatt tgataatcct ccgcctgctg gtggaaataa caacaagcag
    35401 ccacaatttc ctctatcgct tgcggaaaac gcgcttcagg tgaaagggtg taatcaatac
    35461 caatcaccgt acattggctg tagcttgcca gcaggcgcat gatgcgatcg tgggtatcga
    35521 gattgccgag aataaaaccg cctccatgca aataaaatag cgtcgccggg ctatctggct
    35581 gcggacaaaa gagacgtgtt tccacctgcc catattttgt tggaaccatg taagctctgg
    35641 ttgccatttc tggagcgccc gcattccaga atcggcgctc aagcgtgtaa tactgtcgtt
    35701 gctcagcaat cgttcccgtt gcgggccagg acggtaaatc cggctgaaga gtattcacaa
    35761 cggtcttcat ttcagcagaa ataaggtcca gaacaggtag tttgttttcc ggcttcatac
    35821 aaaaactcct ttcaaattac gtcattgtaa ggaaccactg ccatgaaaat gcgatcccgc
    35881 ctgctgatat tgaaactggc tgcgtctcgc gcgctcccgt cagattgtgt taacattcgc
    35941 cgctcagtta accacccgta aaaacaacca tgaaatttcc cggtaaacgt aaatccaaac
    36001 attacttccc tgtaaatgca cgcgatccgc tgcttcagca attccagcca gaaaacgaaa
    36061 ccagcgccgc ctgggtcgta ggtattgacc agacgctggt cgatattgaa gcgaaagtgg
    36121 atgatgaatt cattgagcgt tatggattaa gcgccgggca ttcactggtg attgaggatg
    36181 acgtagccga agcgctttat caggagctaa aacagaaaaa cctgattacc catcagtttg
    36241 cgggtggcac cattggtaac accatgcaca actactcggt gctcgcggac gaccgttcgg
    36301 tgctgctggg cgtcatgtgc agcaatattg aaattggcag ttatgcctat cgttacctgt
    36361 gtaacacttc cagccgtacc gatcttaact atctacaagg cgtggatggc ccgattggtc
    36421 gttgctttac gctgattggc gagtccgggg aacgtacctt tgctatcagt ccaggccaca
    36481 tgaaccagct gcgggctgaa agcattccgg aagatgtgat tgccggagcc tcggcactgg
    36541 ttctcacctc ttatctggtg cgttgcaaac cgggtgaacc catgccggaa gcaaccatga
    36601 aagccattga gtacgcgaag aaatataacg taccggtggt gctgacgctg ggcaccaagt
    36661 ttgtcattgc cgagaatccg cagtggtggc agcaattcct caaagatcac gtctctatcc
    36721 ttgcgatgaa cgaagatgaa gccgaagcgt tgaccggaga aagcgatccg ttgttggcat
    36781 ctgacaaggc gctggactgg gtagatctgg tgctgtgcac cgccgggcca atcggcttgt
    36841 atatggcggg ctttaccgaa gacgaagcga aacgtaaaac ccagcatccg ctgctgccgg
    36901 gcgctatagc ggaattcaac cagtatgagt ttagccgcgc catgcgccac aaggattgcc
    36961 agaatccgct gcgtgtctat tcgcacattg cgccgtacat gggcgggccg gaaaaaatca
    37021 tgaacactaa tggagcgggg gatggcgcat tggcagcgtt gctgcatgac attaccgcca
    37081 acagctacca tcgtagcaac gtaccaaact ccagcaaaca taaattcacc tggttaactt
    37141 attcatcgtt agcgcaggtg tgtaaatatg ctaaccgtgt gagctatcag gtactgaacc
    37201 agcattcacc tcgtttaacg cgcggcttgc cggagcgtga agacagcctg gaagagtctt
    37261 actgggatcg ttaagttatc gtcggttcgt aggccagata aggcgttcac gccgcatctg
    37321 gcatttggct ctcgatgcct gatgcgacgc tggcgcgtct tatcatgcct acatattttt
    37381 catattttac atccggcaac caccgtttac cccgtcacca cttcacccgc cggtggcgtt
    37441 tccagcagtt ccagcatagt acgggcaatt tcacgttcac ccatcactac ctgattcgcg
    37501 ccacgttcgg tgatatacgt cacttcatcg tcataatggg cgcgggcgat aatctcaata
    37561 tccgggtttt tcgcgcgggc agacgccaca atctcacccg cttcataacc gttggggatc
    37621 gtcaggatca gccattttgc acattccaga tgcgccagtt gcataatttc ttcgttcgct
    37681 gcattgccca atacagcgcg gaccccacgc tctcgcagct catcaacacg ggttcgtgac
    37741 gtctcaatca ccaccagcgg aatatcagag gcgagcaatt tctcccccag caggctgcct
    37801 acacgaccgt aacccaccaa tagcgcatgg ttgcaaatat ccactgggat ctgtttctct
    37861 tcttcgattg cctcttccag cgtctgctct tccagcgttt cggtcttcgc cagatatttc
    37921 tccagtagtg cgaacagtac cgggttgagc ataatcgaca ggatcgcccc tgccagtacc
    37981 aggttttgtc cggcctgcgg cagtaaattc aatgccattc ccagtcccgc caggataaac
    38041 gcgaactcac caatctgcgc caggctggcg gcgatggtta atgccgtacg ttgggagtga
    38101 ccaaacagac ggacaaggaa cagggccgcg accgatttac caaacagaat aattgccagc
    38161 gtcgccagca ctgccagcgg ttgctgaatc agaattaacg gatcaaacaa catcccgacg
    38221 gagacaaaaa acagcaccgc aaacgcgtcg cgcaatggca gcgtatcgtg ggcggcacgg
    38281 tgactcagtt cagactcgtt cagtaccatc ccggcaaaga acgcgccaag tgcgaaggag
    38341 acgtcaaaca actccaccgc accaaaggca atccctaacg ccagcgccag caccgacagg
    38401 gtaaatagct cgcgagaacc ggttgccgcg ctgcgtgcca taatccacgg caccagacgg
    38461 cgacctacca gcatcataat ggcgataaat gcgatcactt tgccgatggt gatccccata
    38521 tcgactgcaa gagtggcaaa gcccacatcg ccctgttcca tcattcctgc cactgcgggc
    38581 agcaacacca gcgtcagaac cattaccagg tcttccacaa tcaaccaacc gatggcgatt
    38641 tgcccacgct gactgtcaat taattgccgt tcttcaagtg cgcgcagtaa caccacggta
    38701 ctggcggtgg aaagacataa accgaacacg ataccggtca ttaacgacca gcccagcacg
    38761 gcagagagcg ccatacccag cagcgtcgcc acggctatct gggcgatcgc accgggaatg
    38821 gcgatggcct ttaccgccat caaatccttc agcgaaaagt gcaaaccgac gccaaacatc
    38881 aacagaatga cgcccagttc agccagttcc ggcgcaagct tggtatcggc aacaaagccc
    38941 ggagtgaatg gtcctgccag cacacccgct aacagatatc ccaccagagg agaaatacgt
    39001 agtttattgg ccagcatgcc gaggataaag gcgagcacaa ggccgccaac aatggtggtg
    39061 ataagcgggg tggcgtgatg cattccgtct ccttttcctg gtggttattg tccatttttg
    39121 gccgggaaaa ccaaaattac aggtaatagt ttatgacaat ttcattgatg atgttcatga
    39181 ataattattg aattttgcag aaaaatggaa ttagctgcaa aaaaagcacg gatcggaaaa
    39241 cagaaggcgg tttaacagag gaaaggctta tggcgactgt aaaacgatgc agccaaagtt
    39301 tgcctttggc tgcaatgaaa tcagtcttta tgccggttat caggcaggaa tatggtcaac
    39361 atccccaata gtggcaggaa agcacagatt ttatagacta actcgatgct ggtgtgaggt
    39421 gta
//
LOCUS       DAJTVK010000026        39259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000026 DAJTVK010000000
VERSION     DAJTVK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.026"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO372_001400"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="OO372_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6340941.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO372_001401"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="OO372_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6340942.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="OO372_001402"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="OO372_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6340943.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="OO372_001403"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="OO372_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6340944.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="OO372_001404"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="OO372_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6340945.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="OO372_001405"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="OO372_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6340946.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="OO372_001406"
     CDS             complement(6574..7338)
                     /locus_tag="OO372_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6340947.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO372_001407"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="OO372_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6340948.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="OO372_001408"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="OO372_001408"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6340949.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="OO372_001409"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="OO372_001409"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6340950.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="OO372_001410"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="OO372_001410"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6340951.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="OO372_001411"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="OO372_001411"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6340952.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="OO372_001412"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="OO372_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6340953.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="OO372_001413"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="OO372_001413"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6340954.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO372_001414"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="OO372_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6340955.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="OO372_001415"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="OO372_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6340956.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="OO372_001416"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="OO372_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6340957.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="OO372_001417"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="OO372_001417"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6340958.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="OO372_001418"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="OO372_001418"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6340959.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="OO372_001419"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="OO372_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6340960.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="OO372_001420"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="OO372_001420"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6340961.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO372_001421"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="OO372_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6340962.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO372_001422"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="OO372_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6340963.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO372_001423"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="OO372_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6340964.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="OO372_001424"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="OO372_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO372_001425"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="OO372_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6340965.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="OO372_001426"
     CDS             complement(31717..33003)
                     /locus_tag="OO372_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6340966.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="OO372_001427"
     CDS             complement(33054..33995)
                     /locus_tag="OO372_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6340967.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="OO372_001428"
     CDS             complement(34010..34780)
                     /locus_tag="OO372_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6340968.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35254..36768
                     /gene="caiT"
                     /locus_tag="OO372_001429"
     CDS             35254..36768
                     /gene="caiT"
                     /locus_tag="OO372_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6340969.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36799..37941
                     /gene="caiA"
                     /locus_tag="OO372_001430"
     CDS             36799..37941
                     /gene="caiA"
                     /locus_tag="OO372_001430"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6340970.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38070..39259
                     /gene="caiB"
                     /locus_tag="OO372_001431"
                     /pseudo
     CDS             38070..39259
                     /gene="caiB"
                     /locus_tag="OO372_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9395 a        10791 c        10281 g         8792 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaaatg
    35101 tgataacaat cacagaatgc agcttattga atacccatta tgagttagcc attaacgcgt
    35161 ccacgaggtt aataataatt atatcaaatg ttaacaaaaa taaaacaaac gggaacgcaa
    35221 aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag aaaaacggga
    35281 atagaaccga aggttttctt tccgccgtta ataatcgtcg gcatactttg ttggcttaca
    35341 gtcagagatc tggatgcagc gaatgtcgtt attaatgctg tattcagtta cgtcaccaat
    35401 gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg gttctggctg
    35461 gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cacctgaatt tagtaccgcc
    35521 agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt ctggggatcg
    35581 attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa ctcgacaggg
    35641 gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc gtgggccact
    35701 tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat ggaagtgatt
    35761 cgccccagct cgacactggt gccgctggta ggtgaaaaac acgccaaagg gttgttcggc
    35821 actatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac cagtctgggc
    35881 cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc gcataccctg
    35941 caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg cgtcgcttgc
    36001 ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag cttcctgatg
    36061 ctgggttggg tgttcattgt cagcggtgcc agcttcatca tgaactactt caccgattcg
    36121 gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc catcgctaaa
    36181 ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat ttatgctatc
    36241 cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga actgtgcttc
    36301 ggcatggtgc tggggctgac agcgtcaacc tggatcctgt ggactgtact cggtagtaac
    36361 actctgctgt tgatagataa aaacatcatc aacattccaa atctgatcga acagtacggt
    36421 gtggcgcgcg ccatcattga aacctgggcc gctctgccac tcagcaccgc caccatgtgg
    36481 ggcttcttca tcctctgctt tattgccacc gttacactgg ttaacgcctg ctcttatacc
    36541 ctggcgatgt ccacttgccg cgaagtacgc gatggtgaag aaccacctct gctggtgcgt
    36601 atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct cggcggcctg
    36661 aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt caacattatg
    36721 gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt aacccccaaa
    36781 atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact gtttgtcgcc
    36841 ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga gtgcgaccgt
    36901 gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat cgacagtctg
    36961 ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc cgccgtgtgg
    37021 atggagctgg gacgtctggg ggcaccaacc tatgtgctgt accagttgcc gggcgggttc
    37081 aacaccttcc tgcgcgaagg cacacaagag cagatcgaca aaattatggc tttccgcggc
    37141 accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc cgacgtgggt
    37201 agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg tagtaagtgt
    37261 tttattacca gcagcgccta caccccgtac atcgtggtga tggcgcgcga cggggcttct
    37321 ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg catcaaagtg
    37381 accaaacttg aaaagctcgg tctgcgtatg gatagctgct gtgaaatcac ctttgacgac
    37441 gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa ccgcgtcaaa
    37501 gaagagttcg accatgaacg tttcctggta gccctcacca actacggtac ggcgatgtgc
    37561 gcttttgaag atgcggcgcg ctacgccaat cagcgcgtgc agtttggcga ggctattggt
    37621 cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa ctccatgaaa
    37681 aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc tggcgatgca
    37741 gcgatgtgca aatacttctg cgccaatgcg gcatttgaag ttgtggatag cgcaatgcag
    37801 gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg gcgtgatctg
    37861 cgtgtagacc gcgtctccgg gggatctgac gaaatgcaga tcctgacgct gggtcgcgcg
    37921 gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg cgacgctaat gcgtcttatc
    37981 aggcctacgg gagcacgaat gtaggccgga taaagcgttt acgccgcatc cggcagtcat
    38041 gcactacata aatttttcag gagctaataa tggatcatct acccatgccg aaattcgggc
    38101 cgttggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccgggccg ttcgccgggc
    38161 aaatgttcgc agaatggggt gcggaagtta tctggatcga gaacgtcgcc taggccgaca
    38221 ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg caatttgcac gcgctgtcgt
    38281 taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa accaccgata
    38341 tcttcatcga agccagcaaa ggtccggcct ttgcccgtcg tggcattacc gatgaagtac
    38401 tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt cagtacggca
    38461 ccgaggagta caccaatctt ccggcctata acaccatcgc ccaggccttc agtggttacc
    38521 tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc gccgattact
    38581 tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg cgtgaaaccg
    38641 gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg ggccagtact
    38701 tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa ggtaaagatc
    38761 cctactacgc cggttgcggt ctgtataaat gcgccgacgg ctacatcgtg atggaactgg
    38821 tgggcattac ccaaattgaa gagtgcttta aagatattga cctcgcacat ctactcggta
    38881 cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct tacggcccac
    38941 tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa gttaaagaac
    39001 gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa ctggaaagca
    39061 atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat ggtcgcacct
    39121 gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc tggcgcggaa
    39181 tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac agcgaaaacg
    39241 acattcagga gttggtcag
//
LOCUS       DAJTVK010000027        38337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000027 DAJTVK010000000
VERSION     DAJTVK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.027"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            59..1423
                     /locus_tag="OO372_001432"
     CDS             59..1423
                     /locus_tag="OO372_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6340971.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OO372_001433"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="OO372_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6340972.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="OO372_001434"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="OO372_001434"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6340973.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="OO372_001435"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="OO372_001435"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6340974.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OO372_001436"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="OO372_001436"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6340975.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="OO372_001437"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="OO372_001437"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6340976.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="OO372_001438"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="OO372_001438"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6340977.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="OO372_001439"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="OO372_001439"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6340978.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="OO372_001440"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="OO372_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6340979.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OO372_001441"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="OO372_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6340980.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OO372_001442"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="OO372_001442"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6340981.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OO372_001443"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="OO372_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6340982.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OO372_001444"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="OO372_001444"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6340983.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OO372_001445"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="OO372_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6340984.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OO372_001446"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="OO372_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6340985.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="OO372_001447"
     CDS             13749..14288
                     /locus_tag="OO372_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6340986.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="OO372_001448"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="OO372_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6340987.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="OO372_001449"
     CDS             complement(15648..15995)
                     /locus_tag="OO372_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6340988.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OO372_001450"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="OO372_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6340989.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OO372_001451"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="OO372_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6340990.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OO372_001452"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="OO372_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6340991.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OO372_001453"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="OO372_001453"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6340992.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OO372_001454"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="OO372_001454"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6340993.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="OO372_001455"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="OO372_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR6340994.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OO372_001456"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="OO372_001456"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6340995.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OO372_001457"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="OO372_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6340996.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OO372_001458"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="OO372_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6340997.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OO372_001459"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="OO372_001459"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6340998.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OO372_001460"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="OO372_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6340999.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="OO372_001461"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="OO372_001461"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6341000.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="OO372_001462"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="OO372_001462"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6341001.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="OO372_001463"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="OO372_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6341002.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OO372_001464"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="OO372_001464"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6341003.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="OO372_001465"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="OO372_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6341004.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="OO372_001466"
     CDS             complement(37465..37920)
                     /locus_tag="OO372_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341005.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="OO372_001467"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="OO372_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9474 a        10314 c         9727 g         8822 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactgaaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcaca gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAJTVK010000028        37259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000028 DAJTVK010000000
VERSION     DAJTVK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.028"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..644)
                     /locus_tag="OO372_001468"
     CDS             complement(<1..644)
                     /locus_tag="OO372_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HCR6341006.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(744..1655)
                     /gene="gsiD"
                     /locus_tag="OO372_001469"
     CDS             complement(744..1655)
                     /gene="gsiD"
                     /locus_tag="OO372_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6341007.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1658..2578)
                     /gene="gsiC"
                     /locus_tag="OO372_001470"
     CDS             complement(1658..2578)
                     /gene="gsiC"
                     /locus_tag="OO372_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6341008.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2596..4134)
                     /gene="gsiB"
                     /locus_tag="OO372_001471"
     CDS             complement(2596..4134)
                     /gene="gsiB"
                     /locus_tag="OO372_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6341009.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4154..6025)
                     /gene="gsiA"
                     /locus_tag="OO372_001472"
     CDS             complement(4154..6025)
                     /gene="gsiA"
                     /locus_tag="OO372_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6341010.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6012..6977)
                     /gene="iaaA"
                     /locus_tag="OO372_001473"
     CDS             complement(6012..6977)
                     /gene="iaaA"
                     /locus_tag="OO372_001473"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6341011.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7181..8416
                     /gene="moeA"
                     /locus_tag="OO372_001474"
     CDS             7181..8416
                     /gene="moeA"
                     /locus_tag="OO372_001474"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6341012.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8416..9165
                     /gene="moeB"
                     /locus_tag="OO372_001475"
     CDS             8416..9165
                     /gene="moeB"
                     /locus_tag="OO372_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6341013.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9344..10006)
                     /gene="fsa"
                     /locus_tag="OO372_001476"
     CDS             complement(9344..10006)
                     /gene="fsa"
                     /locus_tag="OO372_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6341014.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10137..11036
                     /locus_tag="OO372_001477"
     CDS             10137..11036
                     /locus_tag="OO372_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6341015.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11042..13474
                     /locus_tag="OO372_001478"
     CDS             11042..13474
                     /locus_tag="OO372_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6341016.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13620..14435
                     /gene="ybiV"
                     /locus_tag="OO372_001479"
     CDS             13620..14435
                     /gene="ybiV"
                     /locus_tag="OO372_001479"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6341017.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14587..15852
                     /locus_tag="OO372_001480"
     CDS             14587..15852
                     /locus_tag="OO372_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6341018.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15819..16979)
                     /locus_tag="OO372_001481"
     CDS             complement(15819..16979)
                     /locus_tag="OO372_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6341019.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17217..18518
                     /locus_tag="OO372_001482"
     CDS             17217..18518
                     /locus_tag="OO372_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6341020.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18533..20896
                     /locus_tag="OO372_001483"
     CDS             18533..20896
                     /locus_tag="OO372_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HCR6341021.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21076..22671)
                     /locus_tag="OO372_001484"
     CDS             complement(21076..22671)
                     /locus_tag="OO372_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6341022.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22890..23810
                     /gene="ldtB"
                     /locus_tag="OO372_001485"
     CDS             22890..23810
                     /gene="ldtB"
                     /locus_tag="OO372_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6341023.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23869..24987)
                     /locus_tag="OO372_001486"
     CDS             complement(23869..24987)
                     /locus_tag="OO372_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6341024.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24984..25451)
                     /gene="mntR"
                     /locus_tag="OO372_001487"
     CDS             complement(24984..25451)
                     /gene="mntR"
                     /locus_tag="OO372_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6341025.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25637..25765
                     /gene="mntS"
                     /locus_tag="OO372_001488"
     CDS             25637..25765
                     /gene="mntS"
                     /locus_tag="OO372_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6341026.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26037..27620
                     /gene="opgE"
                     /locus_tag="OO372_001489"
     CDS             26037..27620
                     /gene="opgE"
                     /locus_tag="OO372_001489"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6341027.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27669..28184)
                     /gene="ompX"
                     /locus_tag="OO372_001490"
     CDS             complement(27669..28184)
                     /gene="ompX"
                     /locus_tag="OO372_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6341028.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28237..28302)
                     /locus_tag="OO372_001491"
     CDS             complement(28237..28302)
                     /locus_tag="OO372_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341029.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28537..29424
                     /gene="rhtA"
                     /locus_tag="OO372_001492"
     CDS             28537..29424
                     /gene="rhtA"
                     /locus_tag="OO372_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6341030.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29723..30226
                     /gene="dps"
                     /locus_tag="OO372_001493"
                     /gene_synonym="pexB"
     CDS             29723..30226
                     /gene="dps"
                     /locus_tag="OO372_001493"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6341031.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30630..31376
                     /gene="glnH"
                     /locus_tag="OO372_001494"
     CDS             30630..31376
                     /gene="glnH"
                     /locus_tag="OO372_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6341032.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31515..32174
                     /gene="glnP"
                     /locus_tag="OO372_001495"
     CDS             31515..32174
                     /gene="glnP"
                     /locus_tag="OO372_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6341033.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32171..32893
                     /gene="glnQ"
                     /locus_tag="OO372_001496"
     CDS             32171..32893
                     /gene="glnQ"
                     /locus_tag="OO372_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6341034.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33010..35235
                     /gene="ybiO"
                     /locus_tag="OO372_001497"
     CDS             33010..35235
                     /gene="ybiO"
                     /locus_tag="OO372_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR6341035.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35232..36158)
                     /gene="rlmF"
                     /locus_tag="OO372_001498"
     CDS             complement(35232..36158)
                     /gene="rlmF"
                     /locus_tag="OO372_001498"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6341036.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36434..36694
                     /locus_tag="OO372_001499"
     CDS             36434..36694
                     /locus_tag="OO372_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6341037.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36959..>37259
                     /locus_tag="OO372_001500"
     CDS             36959..>37259
                     /locus_tag="OO372_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6341038.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9034 a        10059 c         9339 g         8827 t
ORIGIN      
        1 aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga
       61 cgatcaaagc aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac
      121 cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc
      181 ggtccattta cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag
      241 agataatatt tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc
      301 attttcagaa tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc
      361 tttaatgtca gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata
      421 agttcaaagc ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatg
      481 atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc
      541 tttatctttt cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc
      601 tgtgccgctg caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca
      661 aaatcagcag aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc
      721 tttcaagcaa tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa
      781 ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca
      841 gcgacatgcg gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct
      901 ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc tggcggcaga gataatcgag
      961 gtgccaatgc gcatggtgaa aaacaccacg atagacgaga cggtcccagg caagatatga
     1021 cgcaataaaa tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc
     1081 tgtttcaaca ccagcgtgtt gccgcgcacc agtcgggcaa acgcggggat ggaaaaaatg
     1141 gcgactgcga taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc
     1201 agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac
     1261 cagccttcat aatatccagc caacaagccc agcaacgtcc cgatcgccgc accgataaac
     1321 acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg
     1381 tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg
     1441 tcataatcaa aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc
     1501 acaatcaata aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt
     1561 cgccagaatt catgccacgg tgtacgcacc tggtcaggtt tgaccagtgg catggcgttt
     1621 aacaccgcct gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt
     1681 taatggcggc gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa
     1741 gcagaatttc cgcctgaatc accgggtaat cgcgcatttc tacggagtca acgagtaagc
     1801 gtccaagtcc gggccagttg aaaactttct ccaccacgat agaaccaccg agcaaaaagc
     1861 caaactgtaa gcccatcatg gtcactaccg ggatcatcgc gttacgtagc ccgtgtttga
     1921 ggacaaccca ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta
     1981 aaacatcgac aaacgacgcg cgggtaaagc gcgccatcac ggctgccact gccgcgccga
     2041 gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc tcccacggta ggcagccagc
     2101 ccagttcaac ggagaatacc tgaattaaaa gcatccccag cgcaaatgcc ggaaacgaga
     2161 tccccgacac cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg
     2221 cgataattcc cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc
     2281 acagcgttgg cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg
     2341 acaggccaaa atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg
     2401 gctgatccaa ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgagcc
     2461 cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac aaataaaaac accagcaccg
     2521 agacgataaa cagcgtcgga atcaacccca gtaagcgttt gataacgtaa ttaagcattc
     2581 cactccctgc gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgg tgtctggcat
     2641 gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg
     2701 gatccacggc gactcttgcc agatgatatc ctgcgccgcc ttatacaagc gggtcttttc
     2761 cgccggatca ttagttttca gtgcctgagc caggaagtcg tccacctgtt tattgctgta
     2821 aaatgcggta ttaaacagcg tcggtggcca gttttgcgag gcgaacagtg gcgatagcgc
     2881 ccagtccgct tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc
     2941 tttttgccct ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg
     3001 ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt
     3061 gctgtggtta tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt
     3121 cagtaattcg cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc
     3181 gatacttggc ggtaccacac cagtagctgg cgttgcatag cccgcaaagg caactttcac
     3241 cagcgccgga cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg
     3301 cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc
     3361 gatatttttg tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg
     3421 cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt
     3481 tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac
     3541 aaaatcggtc tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc
     3601 aatctccttg ccatattttt ccagcgctgc cggtgaaatc atcgcggtcg ccggatgggc
     3661 aagaatatta ataaacgctg agaacggctg tttgagggta atctttaccg ttgtcggatc
     3721 gatcgcctcc gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg
     3781 atcgctggcc cggtccagat tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg
     3841 gaatttaatg ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata
     3901 actctccgcc agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta
     3961 aaacgatttc gctacggcct gagataacgt atcattcgcg tcatacggat cgagcgtggt
     4021 gaaattcgat cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa
     4081 cgctgtcgca atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt
     4141 ctccgcctga atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat
     4201 gccccggccc gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat
     4261 tgctgggaag atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga
     4321 ccggaactgc cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc
     4381 gccgtggacc aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc
     4441 gctctaccac ggccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga
     4501 gatcgagcaa caagttgata atctgcccgc gaatagaaac atccagcgcc gaaacggctt
     4561 cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc
     4621 gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc
     4681 gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac
     4741 gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt
     4801 cctgaaaaat aaactgaata tcccggcgca atgcctgaag tttgccgggt gacaaggtat
     4861 cgattctctg accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg
     4921 cccgcccggt agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag
     4981 gccagagatc aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca
     5041 aaccgctgcg caaagggaaa cgggtgacaa gattacgcac tcgtaaaaca ggttcgccat
     5101 ccaccaccgt tttctgctcg atgggggggg cctgtttcgc tggatgttca agcgatatca
     5161 acgggaaacg tcggggataa tccaaccctt tcatcgcacc aagttgcgga acagcagcta
     5221 acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt
     5281 ccaccgcctc gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca
     5341 tatcgtgagt gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt
     5401 gcaggatctg cgcctgaata gtgacatcca gcgcggtggt tggctcatcg gcaatcagca
     5461 ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga
     5521 gttgatgcgg ataacgtgaa agaatggttt gtgcctcagg aatgcgtacc tgatccagca
     5581 tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt
     5641 cggcaatctg ttcacccaca gtaaataccg ggttcagcga tgtcatcggc tcctgaaata
     5701 tcatcgccat atccgcaccg cgaacatggc gcatttgtgc agcgctctgc tcgctaagtt
     5761 caatcacttc gcgactgcgc cgctgcaaca gcattttatc gcactgtact aaaccgcccg
     5821 cctgttccaa caggcgcatc aatgccaacg cagtcactga cttaccggag ccggattcgc
     5881 caacaattgc cagcgtctca ccgcgttgca gactaaaaga gagattgcgg accgcagcta
     5941 ttttctgctg gtcctgcata aaggcaatat tcagattttc aaccgccagc acattaccgg
     6001 catcaagttc atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt
     6061 ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg
     6121 acattccctt catggtcgat agcgattaag ccaccgctac cgccaagcgc agggagtttt
     6181 tccattacta cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg
     6241 gcgatgtcat atgccgccag cgcgcggatg aagacttcgc ccgtgccgat acaagaaacc
     6301 gccacactgg cgttattggc gtagcatccg gcacccacta agggactatc gccaactcgt
     6361 ccgggtaatt tattggtcat tccgcctgtg gacgtggctg ccgccaaatt gccgtctaaa
     6421 tccaacgcca cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta
     6481 tggtcgagga cggttgcccc ttccttgcgc gctgccagta gttgttcata acgcaaaggc
     6541 gtggagaaaa tctccggcga gacgcgctcc atgccacgag caaacgcaaa attttctgcc
     6601 ccttcgccaa tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc
     6661 ggattacgca gatgactaac gcccgccacc gcaccggctt tcagggtgtt accatccatc
     6721 acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta
     6781 aacagtggac actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg
     6841 cccgcttcca gcattttctg cccggtttca acaatggcag acaacgcctc aatgtagcgt
     6901 aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca
     6961 atgactgctt tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat
     7021 atcagaattt ttatgaatta ctcattcaat ttttgaatat agaaagatgg atatggcatg
     7081 taaaggcagg agccaacgct acgcccgagg tggcatacag tctgtggcgt ttttctgaca
     7141 taataggcaa attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt
     7201 gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc gtcaccccac tgaccgccca
     7261 ggaaacgctg ccactggtac agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc
     7321 gcttgatgtt ccggggtttg ataactccgc aatggacggc tacgcggtgc gtttagccga
     7381 tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccgtatca
     7441 tggtgaatgg cctgcgggca cctgcattcg tattatgacc ggtgcgccgg tgccggaagg
     7501 ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac
     7561 tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc
     7621 ggttgttttc ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa ttgcttcact
     7681 ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga
     7741 tgaactccag ttgcccggtc agccgctggg cgacggccaa atctacgaca ccaaccgtct
     7801 cgccgtacac ctgatgttag aacagctggg ttgcgaggta atcaacttag ggattatccg
     7861 cgacgatccc catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt
     7921 gatcagttcc ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga
     7981 gctgggggag atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcgttcgg
     8041 taaactcagc aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac
     8101 cttctatcaa ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct
     8161 gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga
     8221 tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg
     8281 acatcagggt tcacatatat ttagctcctt tagtctcggc aactgcttta tcgtgctgga
     8341 acgcgatcgc ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt
     8401 gttcggaggc ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa
     8461 atcattctgc gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg
     8521 atcgtcggcc tcggtggact cggctgcgca gcttcgctgt atctggcaag cgccggtgtc
     8581 ggcaacctga cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagaca
     8641 ctgcacagtg atgccacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg
     8701 cggatcaacc cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt
     8761 gcagcattga ttgctgaaca cgatctggtg ctcgactgta cggataacgt tgcggtacgt
     8821 aatcaactga acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt
     8881 cgtatggaag ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc
     8941 cttagccgtt tgtttggcga gaacgcatta acctgcgtag aagcaggcgt gatggcaccg
     9001 ttgatcggcg taattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat
     9061 ggcaaaccga ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa
     9121 atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc
     9181 ctgataagac gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga
     9241 acgccttatc tggcctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac
     9301 tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc
     9361 caaacgctcc ctgccagtcc tgctcaaact tcgccacagc ggcttccacc gccggatagc
     9421 taatcatctg ttgtgccaca tccagtggca gagtaattga ttcacatcct gccagtaagc
     9481 agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat
     9541 gcattttcaa taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat
     9601 caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcaaccctt
     9661 gtgctgcgcc atataccgcg gttcccagcg ttggaatccc ttccgctttt aacatcttaa
     9721 tagctgccag cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac
     9781 gcaatttacg tgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca
     9841 gacgcccctg accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggttttt
     9901 tacccgcggc gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca
     9961 gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa
    10021 tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat
    10081 caatctaact ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcgactatga
    10141 ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc
    10201 ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc
    10261 aggatctgct gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg
    10321 cgccggaagt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc
    10381 cggagcatct gacggcgtta accgactgct gtccgacaca ggcattaacc gtgtgtggtg
    10441 aagtgaaaag cgttgaggag atcatgacga ccgttctgcg cgataaaccg ttttacgatc
    10501 gcagcggcgg cggtttaacg ctttcgggtg gtgagccctt tatgcagccg gaaatggcga
    10561 tggcgctact gcaagccagc cacgaggcag gcattcatac tgcggtagaa acctgtctgc
    10621 atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt
    10681 taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagagtgc
    10741 tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga
    10801 ttcagggctt taatgccgac gaaacctctg taaaagctat taccgatttt gccgccgacg
    10861 agctgcacgt tggcgaaatt catttcctgc cctaccacac gctaggcatc aacaaatatc
    10921 acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg
    10981 actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac
    11041 catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct
    11101 ggtgcatatt gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta
    11161 tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct
    11221 ggcgaatcgc accatctgga tcaaacacga tgagttgatc attggcaacc aggcaagcga
    11281 agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga
    11341 tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgcgttct
    11401 gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt
    11461 taccgatgag caaaaaggtc tgctggcgac cggaatcatt aaagcggaag gcaatatgac
    11521 ctccggcgat gcgcacctgg cggtgaattt cccgctgctg ctggaaaaag gtcttgatgg
    11581 tctgcgcgag aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtac tggaagattt
    11641 gcacggcgaa cagttcctga aagcgattga tatcgtgctg gtggcagtca gcgaacacat
    11701 tgaacgtttt gcagccctgg cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg
    11761 cgatgaactg ctggcgatag cagaaaactg cgatcttatc gcccaccagc cgccgcagac
    11821 tttctggcag gcgctgcaac tgtgttactt catccaattg atattgcaga tcgaatctaa
    11881 cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc tacccgtact atcgccgcga
    11941 cgttgaactc aaccagacac tggatcgcga acacgccatc gaattgctgc atagctgctg
    12001 gctgaaactg ctggaagtga acaagatccg ctccggctca cactcaaaag cctctgcggg
    12061 aagtcctctg tatcagaacg tcaccattgg cggacaaact ctggttgatg gtcaaccaat
    12121 ggacgcggtg aatccactct cttacgctat cctcgaatcc tgcggtcgcc tgcgttccac
    12181 tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt aatgatttcc tcgacgcctg
    12241 cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat
    12301 cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactacg cagcgattgg
    12361 ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat
    12421 caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa
    12481 agtgttcctg ccacaagaaa aagcgttgtc ggcaggcaac tttaacaatt tcgatgaagt
    12541 aatggacgcg tgggatacgc aaatccgtta ttacacgcgt aaatcaatcg aaatcgaata
    12601 tgtcgtcgac accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga
    12661 tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc
    12721 tggcctgcag gttggcattg ccaacctcgg caacagcctg gcggcagtga agaaactggt
    12781 atttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga
    12841 tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa
    12901 cgacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact
    12961 gaaacagtac cataacccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg
    13021 tacctcgtct atctccgcta acgtaccgtt tggcgcgcaa actatggcaa caccggacgg
    13081 gcgtaaagcc cacaccccgc tggcagaagg cgcaagcccg gcctccggta ctgaccatct
    13141 tggccctact gcggtcattg gctcagtggg taaactgcct acggcagcga ttctcggcgg
    13201 cgtgttgctc aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa
    13261 actgatgatc ctgctgcgta ccttctttga agtgcataaa ggctggcata ttcagtacaa
    13321 catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct
    13381 ggtagtgcgt gtcgcgggct attccgcctt cttcaccgcg ctctctccag acgcacagga
    13441 cgatatcatc gcccgtactg aacatatgct gtaatctcct cggcccggcg gcgtcgtcgc
    13501 cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg
    13561 ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta
    13621 tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt
    13681 acaaccaacc acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg
    13741 ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg
    13801 agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc
    13861 acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc
    13921 aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag
    13981 catttgtcgc actgatggca aaacactacc atcgcctgaa agctgtaaaa gattatcagg
    14041 agattgacga cgtactgttc aagttttcgc tcaacctgcc agatgaacaa atcccgttag
    14101 tgatcgacaa actgcacata gcgctcgatg gcattatgaa acccgtcacc agtggttttg
    14161 gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga
    14221 aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg
    14281 agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac
    14341 aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg
    14401 cggtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc cgtgcgctcc
    14461 ggttttccct tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt
    14521 atttataact tatattgtcg catgcaaatc agaatggttt cgaatgaaag ttaatgagag
    14581 gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc
    14641 cgtcagatga agcacgcgct gcgggcgcag cttggcgacg tccagcagat ctttaatcag
    14701 ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat
    14761 gccgtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag
    14821 cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa
    14881 gcgctaggct gggatcagtc gatgctggac tatctggacc gcaaccgctt tgacgaggtc
    14941 tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac
    15001 cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg
    15061 cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg
    15121 gatgtgagcg tcatctaccc tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa
    15181 ggtcttggag cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag
    15241 cgcgttttcg ccaacgtctt taatggcaat ctggcgcaat atgatccctg gcatgcggca
    15301 catcgtacgg aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg
    15361 acattccaag gctggacagc gctctctaat atgctgcctg gtcaggggct gctgcacgtt
    15421 gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg
    15481 gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat
    15541 ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg
    15601 tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac
    15661 gtgatgtaca ttcctgcggc accaatgtgc gagaaaaatc ttgcctacgc gcacaaggtg
    15721 aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca
    15781 aactgggaag gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttggga
    15841 atcgacagct gagtgtcgta cagcccttcc cgttccacgg cggtgttccc ttttcctggg
    15901 cgaacccgcc gcactgactc ccgcaccgcc aatcgtccgt gcagcagcag cgagcccacc
    15961 ggagcatccg ggcgaatcag ccgctgttgc aacatttgca ccgcttcatt gcctagctca
    16021 tcgcgtggca catgcacagc cgtcagggga acatcctcga ttgccgcaag gttaaagccg
    16081 tcgatactca tcaccgagac atcctgtggt acgcgcagac cacgacgatg tagcgcattt
    16141 accgttcccg ccgccataaa atccccgccg accagaaacg ccgtcggcaa cgtcttgcca
    16201 ttggcggcat ccagccacgc tccgaccttt tcttccgctt ctttagcgct aaaactgctg
    16261 atattcagca aatgacggtc atggtgaaag gccagattat gctgacgcca tgattcttta
    16321 atccctgcaa accgctgctc catagtgtag cggcgcaggc acatcacatt catcacctcc
    16381 cggtgaccca tttcaaacag atagcgggcg gcgaacgcgc caattaaccg atgatccggc
    16441 gcaatcgccg ggaggcgcat tcgttcatca cggcagttga tcagcacaca aggcttagcg
    16501 agatcggcgg ccagatcatg gatgtgcgga tcgtcaatcc ccagcaggat cgctgcctgc
    16561 gtatctgctt cattcatccg cgagagaaag agcgcgggat cgctgtcaaa ctcctccagc
    16621 gcgcagtaac gcagccgcac ttcatgcggc gataacgctt tgctgatgct ctggatcaca
    16681 cggtaataaa agatatcaga tcgttggtca aaagcgcgtt gcggcgcgaa aataatcagg
    16741 ttgttcagca acatacgacc tgctgccatg ccatccatca cgcccaactc acgcgcgcag
    16801 gccagcacct gttcgcgagc gcgtgcactg gtattcgctt tgccagccag cacccgcgaa
    16861 accgtactcg gcgatagccc ggtacgtgcg gcgatatcgg cgattttcag cttcttgtcc
    16921 attttgtgat ctgcctctaa ttcggaaatg acagaatttt catgagcaaa aaagcccatt
    16981 ctgtctggca ttaaatccat ttcagtaatg tatatcagtc actcatggaa aaacgtgcac
    17041 aaaacccgct ttctttcgct tttagggtct cttactgtga attggtagaa caacttccat
    17101 acaaaaataa tggaaaaacg aacaataaag actttattta tcaaaaagtt aaaagtggtg
    17161 taacaactcc attctgacgt tgccgcacaa accctatacc attctgtgga gtgcatatga
    17221 gtcagggtat caataacgat atgactgcca gtaaaccgcg ccatatcgtc aaaaatctgc
    17281 gttggtggat actggtcctt tttttgctcg gtgttactgt caactacatc acgcgtaact
    17341 cgctgggtat tctcgccccc gaactgaaaa gcagcctagg gattaccacc gaacaatatt
    17401 cctggattgt aggtgctttc cagctcgcct ataccctatt ccaaccgttg tgcggatggc
    17461 tgattgacgt cattggcctc aaactgggct ttatgatctg cgcgggactg tgggcactgg
    17521 cctgtctgat gcatgcgggg gcgggaaact ggggacatct ggcgctgttg cgcttcttta
    17581 tgggcggtgc ggaagccgcg gcaaccccag caaacgccaa aaccatcggt gaatggtttc
    17641 caaaatcaga acgcccgatc gccgcaggct gggcaggcgt cggtttttcc atcggtgcca
    17701 tgctggcccc accgattatc tattttgccc acgcttcatt cggctggcag ggtgcattta
    17761 tgttcactgg catcctggcg atgctgtggg tggtgctgtg gtgggcgttc tatcacagcc
    17821 cggataagca tcctaacctc agcaaagccg agctggactt tattcgccag gataacgaag
    17881 cgcccccggt caagttaccc tttctgaccg cactgaaaac cgtgggcaaa aataaacgct
    17941 tttacggtat cgctatcccg gcctttatgg cagaaccggc gtgggcggtg atgagtttct
    18001 gggtgcctct atacctgtcc aaaacctatg gcatggacct gaagcagatc gcattattcg
    18061 cctggttgcc gttcctcgcc gctgatcttg gcagtattgc cagcggctat ctgaccaaac
    18121 tttacacccg cctgtttggg ctgagtcgtg tgaactccgt tgtcgcatca tcggtcagtg
    18181 gcgcattctt gatggtttcg ctggcggtga tggcctttac caataacccg tatatcgcca
    18241 tcgtccttat ctccatcggc ggcttcggtc accagatcat ctcctgcatg ctgagcgcac
    18301 tggtggtgga atccttcgac aaaggtcaga tggccactgt taacggcatg cgcggttctg
    18361 ccgcgtggat cgcaagtttc ctgttctccc tgattatcgg ggttaccgct gacaaaatcg
    18421 gctttaaccc cctgtttatc gccatgggct tctttgacct gattggcgct ttcttcctgg
    18481 tcacctttat tgctgaacgt cgcgcaaaac gcgcctgaga gtggatgtaa acatgaaaac
    18541 gctgaaaaac tggaccctgc acaaacagtc tgcccaccac gttgaactgc tggtcgatgg
    18601 ccagcatatc ctgtgcctgt acgtgctgga agaaaatctg ttccgcgtgc tgttaaaacg
    18661 caaaggcgag ctggcgctag atcgcacctg gagcattgct ccggcgcagg atgtgccatg
    18721 ggaagggcgc agccgcgaag atctcagcgg ctttagcctg cccgcgtgga cagtgatcca
    18781 gcacgacgac accgtggtta tcgccaccga gtcactgcgt gcaaccgttc accagccgct
    18841 gtggctggag tggcactatc gtaacaacga aggcgaatgg ctgccgttgg tgaatgaccg
    18901 cccgaccagc gcttatctgc tgaatgccca cggcgatggc gtggcacact atcagagccg
    18961 tcgtaaagat gaacgctttt atggcctcgg agataaagcc ggcgatctgc aacgcaacgg
    19021 taaacgttac gagatgcgta acctcgacgc gatgggctac aacgccgtta gtactgaccc
    19081 gctgtacaaa cacattccat tcaccatcac ccgccgcgat gacattagct tcggcctgtt
    19141 ttatgacaac ctcagcagct gctggctgga tctgggcaac gagatcgaca actaccacac
    19201 cgcctatcgc cgctggcagg cggaagcggg tgatatcgac tactacctgt ttaccggcga
    19261 acgcgtactt gatatcacca aagccttcgt acgcctgacc ggaaaaaccc tgttcggacc
    19321 gaaatggagc ctgggctaca gcggttcaac tatgcactac accgatgcgc cggacgcaca
    19381 aaatcagttg atgaacttta tccgcctgtg tgaagaacat gcaattccct gcgattcgtt
    19441 ccagctctct tccggttata cctcaattaa cggcaaacgc tatgtgttca actggaacta
    19501 cgacaaagtg ccgcagccga aagtgatgag ccaggcattt cacaatgctg gtttgcacct
    19561 ggccgccaat atcaagccgt gcctgttgca ggatcaccca cgctataacg aagtggcaga
    19621 aagaggactg ttcatccgtg attcagaaac agatgaacca gaacgttcca gcttctggga
    19681 tgacgaaggt tctaacctcg agtttactaa tccgcagacc atccagtggt ggcaggaagg
    19741 cgtgaccaca cagcttctgg agatgggtat cgactccacc tggaacgaca acaacgaatt
    19801 cgaagtatgg gacggtgaag cacgctgtca cggctttggt cagacgatcg cgatcaaaca
    19861 tattcgccca gtgatgccac tgctgatgat gcgagcctcg atggaagccc agcagcgttt
    19921 cgctccggaa aaacgtccgt acttgatctc ccgctccggc tgcgcgggaa tgcagcgcta
    19981 cgtgcagacc tggagtggcg ataaccgcac tagctgggac actctgcgct acaacacccg
    20041 catgggtctg gggatgagcc tctccgggct gtataacctc ggtcacgacg tcggcggttt
    20101 ctccggtgat aagccggacc cggaactgtt tgtacgctgg gtacaaaacg gcgtgatgca
    20161 tccgcgcttt acgattcact catggaacga tgaccatacc gtaaacgaac cgtggatgta
    20221 tccgggcgtc actccggcga tccgcagcgc tattgagttg cgctatcgcc tgctgcccta
    20281 cttttatacc ctgatgtggc aggcatacgc cgatgatgaa ccaatgctac gtccgacttt
    20341 ccttgaccac gagcacgacg ttcagacttt tgaagagtgt gatgacttcc tgcttggtcg
    20401 cgatattttg gttgccagcg tcgtagagca aggcgagcgc cagcgccgtg tatggctgcc
    20461 agataacgaa actggctggt acgatttcta caacggcgaa tggttcagtg gcggtcagtg
    20521 gatcaccctc gacgcaccgc tggagaaact gccgctgatg gttcgtgccg gggctggtct
    20581 accgctcagc aaacgcatca cctatgtgag cgcagaacaa gacgataccc gcgaactgaa
    20641 actgttcccg ctgaaaggtg tgggcacaac gtccggcctg ctgtttgaag acgatggcga
    20701 aagctggggc taccaaactg gtaatgcgct ttgggtggaa tgggaaatgg tttgcgatgg
    20761 ggcgaccatc aacctgaaag tcaacgcgcg cggggattat cgtcctgcat ggagtgcact
    20821 gaaagtgtca ttaccattgg aagaaaaacg cacgctgctg gtcaatggtg ttgaagggag
    20881 tgaatggatg cgttaaatca ttaccggggc cgggaggcga ctgcacctta cccggtccag
    20941 ccatcgaatc ctttacctca gtatttcacg ctgcaggaag gcagcaaacc tgtaaacccc
    21001 gggagcttaa ataagtaagt gaccggggtg aacaggtgaa gccaatgcat ctgccgcatg
    21061 aaagacggcg gggaattaag cgtcgatccc tttactacgc aggtaatctt cgtaattacc
    21121 gctaaagtcg atcgcgcgtt ccggggtgat ttccagaatg cgggtcgcca gggagcttac
    21181 gaactcacgg tcgtgtgaaa caaagatcag cgtgccctga tacagttcca gcgccatgtt
    21241 cagtgactcg atggattcca tatccaggtg gttggtcggt tcgtccatga tcagaatgtt
    21301 cggcttctgc atcattaact taccaaacag catccgccct ttttccccac cggaaagcac
    21361 tttagctggc tttttgatgt cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac
    21421 cgcctgctcg tcatcgcctt cctgcttcca ctgactcatc cattcgaaca cggtcagatc
    21481 attttcaaac tcatattcgt ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca
    21541 ttttacggtg ccgctgtccg gttgcagatc gcccaccagc gttttcagca gcgttgattt
    21601 accgacgccg ttggtaccca gtaccgcaag tttttcaccg acctccagca gcaggttgag
    21661 atttttaaac agtggaccgt tatcaaaccc tttggtcaga ccttccactt ccagcgcgtt
    21721 acggaacagt ttcttatcct gttcaaaacg gatgaacggg ttctgacggc tggaggcttt
    21781 cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt
    21841 cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac tcagcaatct gcgctttctt
    21901 cttggcgtta tcggccagca gacgttcacg cgcctgggtc gccgccgtca tgtactcatc
    21961 gtagttaccc ggataaacgc gcagctcgcc gtaatccaga tccgccatgt gggtacagac
    22021 catgttaagg aagtgacggt cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag
    22081 cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag
    22141 gagaatctcc ggatcagcaa acagcgcctg cgccagaagc acacgcagtt tccagccagg
    22201 agcaacttca ctcatcgggc cgtagtgctg ttccactgga attcccacgc caagcaacag
    22261 ttcaccggcg cgagcttccg cagagtaacc gtccatttcg ccgtatttaa cttccagatc
    22321 ggccacttta tagccgtctt cctcactcat ttccggcaaa gcatagatgc ggtcacgctc
    22381 ctgcttcact tcccacaact ctttatgccc catgatcacc gtatccagca cagtgaactc
    22441 ttcaaaggca aactgatcct gacgcagctt accaatgcgc tcgttgggat cgagggaaac
    22501 gttacccaaa gtcggctcta agtcgccgcc gaggatcttc ataaaggtgg atttaccact
    22561 accgttcgcg ccaatcaggc cgtaacggtt gccgccgccg aatttgacgg aaatgttttc
    22621 aaacaacggc ttactgccga actgcatggt gacgttactg gaaactaaca caggcgtatc
    22681 ctgaaaagag atatgacaaa ccgcgcatta tgccataagt ctgaaagaag atcctgctcc
    22741 tcgcttaagg tctacactca agccaaaggc aaaaaatgtg atttcgtaca catctgattt
    22801 cactgtgagc tggaatgaac ttataatgcg cttccaatac tctaatattc tcaacccaat
    22861 ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag
    22921 cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc ggtaacttat cctctgccaa
    22981 ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac cattcctgaa ggtaacactc
    23041 agccgctgga gtattttgcc gcggagtacc agatggggct ttccaatatg atggaagcga
    23101 acccgggtgt ggataccttc ctgccgaaag gcggtactgt actgaacatt ccgcagcagc
    23161 tgatcctgcc ggataccgtt catgaaggca tcgtcattaa cagtgcagag atgcgtctgt
    23221 attactatcc gaaagggacc aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag
    23281 gcaaagatac gcctatcaac tggaccacca aagttgagcg taagaaagca ggcccgacct
    23341 ggacgccgac cgccaaaatg cacgcagagt accgcgctgc gggcgaaccg cttccggctg
    23401 tcgttccggc aggtccggat aacccgatgg ggctgtatgc actctacatc ggtcgcctgt
    23461 acgctatcca tggcaccaac gccaacttcg gtatcggcct gcgtgtaagt catggttgtg
    23521 tgcgtctgcg taacgaagac atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg
    23581 tacagtttat tgatgagccg gtaaaagcga ccaccgagcc agacggcagc cgttatattg
    23641 aagtccataa cccgctgtct accaccgaag cccagtttga aggtcaggaa attgtgccaa
    23701 ttaccctgac gaagagcgtg caaacagtga ccggtcagcc agatgttgac caggttgttc
    23761 ttgatgaagc gattaaaaac cgctccggga tgccggttcg tctgaattaa tcttcaaaac
    23821 ttaaagcaaa aggcggactc ataatccgcc ttttttattt gccagaccct agttggccgg
    23881 gagtataact aacaaaacat atcccaccaa cgccgcccac aacagcatcg gtattgaata
    23941 gagatggaaa cgccaccaga tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc
    24001 cagcgatccg ggtaataacc caaagccacc tacgtttacc gcccatacca gtaataaaga
    24061 cggcggcaca tagttcagca acaatatggt actcggcacg ttactgatca cctgcgataa
    24121 accgattgcc gttaaccata acccgggttc agatagatga ctcacgttac ccaacacgcc
    24181 ttgcaacgct ggaagctggg tcagtaaatg gacgtcgata aacatcgcca taaacaccag
    24241 cagcagcgtc cagtccacac tgagcaccac gcgacgtgcc agcagcgcaa agcctgccgc
    24301 cacaatcacc agtccccaca gctcttgttt gaactccagc gccgtcagaa agacgatata
    24361 cagccccaga caactccaca ccagccgcgg tttccactcc ggtgtttgca cccccgtatg
    24421 gtattgcagc gcctttccag ggaaacaaca ccagcacagg agcaggagcg tcagcatcat
    24481 tgcgccagcc agcggtgcca tttgggcaat aaatccggca aacgaaagac cagaacgtcc
    24541 ccagataaga atattttgcg ggttgccaat tggcgtcagt agcgaaccag cgttgactgc
    24601 cagcgcctca aaaataatca gccgattaac cgggatctca cacagtcttt ttagcgtgat
    24661 agtcagcgga acaacaataa acagcgcgac atcgttggtc agaaaggtag aaagcagcgc
    24721 cgccgccagc accataaaca tcgccagccg acgctccgta gcaaagcggc gcaccatttt
    24781 gcgccccagc acatcaaaat aaccgcttaa ctccacacct ttggtcagca gcatcaggcc
    24841 gcttaaggtg atgatggtgt gccagtcgat agcagcaggc caggatttcg gtgcaaaggg
    24901 cacgaaaaag cttaatccga taccaacaag aattaataac tgaaaaaaac gatcgccttg
    24961 cagcgtgcgt aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa
    25021 gcgtcgagcg tctcttcact aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt
    25081 tccggactga cgcccaacac caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt
    25141 tcctgcgcca gcttctctcc ttctgccgtt aaaaacacgc cacgccaggg gatcatttca
    25201 atcagcccca tggttgccag ccgcttaagc attttagcca ccgtcggttg cgaaactccc
    25261 agacgagcag ccatgtccac ctgacgagct tcccccactt ccctgatcaa gtcagaaatc
    25321 agctcaacgt aatcatcaat aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct
    25381 tcaacgtgct cttccacgtt cactaactgc gtcacttttt ttgctgttgg cgtacctgcg
    25441 cgacgactca ttgtgcttcc tcattatggt gacgcataca tagcgttctc atttcgtgcg
    25501 cacattgtaa accagagttg cgaaggtaca aaaaattaac gttttagcaa tagctacata
    25561 atatagcctg tgctatatct gtatgtaatg caatcatccc tcaaggatcg acgggattag
    25621 caagtcagga ggtcttatga atgagttcaa gaagtgtatg cgcgtgttta gtcattctcc
    25681 ctttaaagta cggttaatgc tgctctctat gttgtgcgat atggtcaaca acaaaccgca
    25741 gcaagataaa ccttccgata aatagcggcg tcgcggtacg ccgcttcact cctgctttta
    25801 tgcaggcata acgcgttttg gtctgaaaaa ccccactttt tgtcggattt gcaatcccct
    25861 tcgcaaaaga tttgttcgtc agtagttgac ctgaacggcg gctcgctcta tcttcttgca
    25921 gccctacgta tattgcggct cgcggatgcg gacccctttc ccctcttcac gcactcttgc
    25981 aggtattgac ccttgacgcc agggtaagca catggcgttt gttacgatag tggcatatga
    26041 atttaaccct caaagaatcg cttgttaccc gtagccgggt atttagcccg tggactgcgt
    26101 tctacttttt acagtcgcta ttaattaacc tcggcttagg ttaccccttc agtttgctct
    26161 acaccgctgc gtttacggct attttgcttt tgctatggcg aacattgcct cgcgtacaaa
    26221 aagttctggt cggtgtcagt tcgctggtgg cggcttgtta tttccctttt gctcaggcct
    26281 acggcgcgcc taatttcaat acattgctgg cattgcactc caccaatatg gaagagtcga
    26341 ccgaaatcct gacgattttt ccgtggtaca gctacctggt cggcttattt atttttgcgc
    26401 tcggcgtaat agcaatcagg cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg
    26461 acagcctgtg tctggtattc agtgtggcga cattttttgt tgctcccgtg caaaacctgg
    26521 cctggggtgg cgtatttaaa ctgaaagata ctggctatcc ggtatttcgt tttgctaagg
    26581 atgtcatcgt caataataac gaggtgattg aagagcaaga acggatggca aaactttccg
    26641 gaatgaaaga tacctggacg gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg
    26701 tgatcggcga aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac tgggacaata
    26761 ccccgtttgc cagcagcgtt aacggtttga tatttgctga ctacattgcc gccagtggct
    26821 ccacgcagaa atcacttggc ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc
    26881 aggataactt tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc
    26941 agggccaaat cggcgaatac gatactgcta tcgccagcat cgccaaacgt gcagatgaag
    27001 tgtacttcct gaaagaaggt aatttcgaag cagataaaaa cacgaaagac gaagcgttac
    27061 tggatatgac cgctcaagtg ctggcgcaag agcactcgca accgcagctg attgttctgc
    27121 atctgatggg ctcgcatccg caggcctgcg acaggacaca aggcaaatac gaaacctttg
    27181 tgcaatcgaa agaaacgtcg tgctatctct ataccatgac gcaaacggac gatttactgc
    27241 gcaagctgta cgatcagtta cgcaacagcg gcagcagctt ctctctggtt tacttttctg
    27301 accacggtct ggcctttaaa gagcgcggca aagacgtgca ataccttgcc catgatgata
    27361 aatatcagca aaatttccag gtgcctttta tggtcatttc cagcgacgat aaagcgcatc
    27421 gggtgattaa agcccgccgc tcagccaatg acttcttagg ctttttctcg cagtggacgg
    27481 gaattaaagc gaaggaaata aatatcaaat acccgtttat atctgagaag aaagccgggc
    27541 cgatatacat caccaacttc cagttacaga aggtggatta caaccatctc ggaaccgata
    27601 ttttcgaccc gaaaccttaa aacaaaaaat ccgccccgag aggcggattt tttatatcac
    27661 caaagtgatt agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga
    27721 atacggctct gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga
    27781 ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg
    27841 gtctggaatt taccataacc cacccccact acaccgtaga tgcttgccca gtcgttaatg
    27901 cggtaagccg gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt
    27961 gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct
    28021 tcttcatagc ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg
    28081 ctctgtgcgt aaccgccagt tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa
    28141 gccagaactg cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg
    28201 attcaaataa gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt
    28261 gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa
    28321 atttaaatca ctttttcaga gaactgcgta agtattacgc atgttttccc tgtcattcat
    28381 ccagattatt cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa
    28441 acgggggaaa attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca
    28501 cgctttactt aagtctagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg
    28561 ccggtctggt taccaatagt catattgctc gttgccatgg cgtctattca gggtggagcc
    28621 tcgttagcta agtcactttt tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg
    28681 gcattaggaa cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa
    28741 gagcaacggt taccgctgtt gttttacggc gtttcgctgg gtgggatgaa ttatcttttt
    28801 tatctttcta ttcagacagt accgctgggt attgcggtgg cgctggagtt caccggacca
    28861 ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg
    28921 gttcttggtc tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaacc
    28981 ggctgtgcgc tggcactggg ggccggggct tgttgggcta tttacatttt aagtgggcaa
    29041 cgcgcaggag cggaacatgg ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta
    29101 attttcgtgc caattggagc gcttcaggct ggtgaagcac tctggcactg gtcggttatt
    29161 ccattgggtc tggctgtcgc tattctctcg accgctctgc cttattcgct ggaaatgatt
    29221 gccctcaccc gtttgccaac acggacattt ggtacgctga tgagcatgga accggcgctg
    29281 gctgccgttt ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg
    29341 ctcggcgcta tcatcgccgc ttcaatgggg tctacgctga cagtacgcaa agagagcaaa
    29401 ataaaagaat tagacattaa ttaaatttac atttctgcat ggttatgcat aaccatgcag
    29461 aatttctcgc tacttttcct ctacaccgtc tttatatatc gaattatgca aaagcatatt
    29521 tattccgaaa attcctggcg agcagataaa taagaattgt tcttatcaat atatctaact
    29581 cattgaatct ttattagttt tgtttttcac gcttgttacc actattagtg tgataggaac
    29641 agccagaata gcggaacaca tagccggtgc tatacttagt ctcgttaatt actgggacat
    29701 aacatcaaga ggatatgaaa ttatgagtac cgctaaatta gttaaatcaa aagcgaccaa
    29761 tctgctttat acccgcaacg atgtctccga cagcgagaaa aaagcaacag tagagttgct
    29821 gaatcgccag gttatccagt ttattgatct ttctttgatt accaaacaag cgcactggaa
    29881 catgcgcggc gctaacttca ttgccgtaca tgaaatgctg gatggcttcc gcaccgcact
    29941 gatcgatcat ctggatacca tggcagaacg tgcagtgcag ctgggcggtg tagctctggg
    30001 gaccactcaa gttatcaaca gcaaaacccc gctgaaaagt tacccgctgg acatccacaa
    30061 cgttcaggat cacctgaaag aactggctga ccgttacgca atcgtcgcta atgacgtacg
    30121 caaagcgatt ggcgaagcga aagatgacga caccgcagat atcctgaccg ccgcgtctcg
    30181 cgacctggat aaattcctgt ggtttatcga gtctaacatc gaataaatcc atcgctgatg
    30241 gtgcagaact ttagtacccg ataaaagcgg cttcctgaca ggaggccgtt ttgttttgca
    30301 gcccacctca acgcacttat ttagtgcatc catctgctat ctccagctga ttaagtaaat
    30361 tttttgtatc cacatcatca cacaatcgtt acataaagat tgttttttca tcaggtttta
    30421 cgctaaataa tcactgtgtt gagtgcacaa ttttagcgca ccagattggt gccccagaat
    30481 ggtgcatctt cagggtattg ccctataaat cgtgcatcac gtttttgccg catctcgaaa
    30541 aatcaaggag ttgcaaaact ggcacgattt tttcatatat gtgaatgtca cgcaggggat
    30601 cgtcccgtgg atagaaaaaa ggaaatgcta tgaagtctgt attaaaagtt tcactggctg
    30661 cactgaccct ggcttttgcg gtttcttctc atgccgcgga taaaaaatta gttgtcgcga
    30721 cggataccgc cttcgttccg tttgaattta aacagggcga taaatatgtg ggctttgacg
    30781 ttgatctgtg ggctgccatc gctaaagagc tgaagctgga ttacgaactg aagccgatgg
    30841 atttcagtgg gatcattccg gcactgcaaa ccaaaaacgt cgatctggcg ctggcgggca
    30901 ttaccatcac cgacgagcgt aaaaaagcga tcgatttctc tgacggctac tacaaaagcg
    30961 gcctgttagt gatggtgaaa gctaacaata acgatgtgaa aagcgtgaaa gatctcgacg
    31021 ggaaagtggt tgctgttaag agcggtactg gctccgttga ttacgcgaaa gcaaacatca
    31081 aaactaaaga tctgcgtcag ttcccgaaca tcgataacgc ctatatggaa ctgggcacca
    31141 accgcgcaga cgccgttctg cacgatacgc caaacattct gtacttcatc aaaaccgccg
    31201 gtaacggtca gttcaaagcg gtaggtgact ctctggaagc gcagcaatac ggtattgcgt
    31261 tcccgaaagg tagcgacgag ctgcgtgaca aagtcaacgg cgcgttgaaa accctgcgcg
    31321 agaacggaac ttacaacgaa atctacaaaa aatggttcgg tactgaaccg aaataataac
    31381 gctacacctg taaagcgcac tggcagttcc ctctcccctc tggggagagg attagggtga
    31441 ggggcgcaaa cccgctccgg ggccattaat taccctgaat ttgattattt acaccacggt
    31501 aacaggaaca acatatgcag tttgactgga gtgccatctg gcctgccatt ccgcttctga
    31561 ttgaaggtgc caaaatgacc ctgtggattt cggtcctcgg tctggctggc ggtctggtaa
    31621 tcggattgct ggcaggtttt gcacgcacct tcggtggttg gatagccaac cacgtcgcgc
    31681 tggtctttat tgaggtgatc cgcggcacac ctatcgtcgt ccaggtgatg tttatttatt
    31741 tcgccctgcc gatggcgttt aacgacttac gcatcgaccc atttactgcg gcggtggtca
    31801 ccatcatgat caactccggc gcgtacattg cggaaatcac gcgtggtgcg gtgctgtcta
    31861 ttcacaaagg ttttcgtgaa gcaggactgg cgctcggtct ttcacgttgg gaaaccattc
    31921 gctacgtcat tttaccgctg gcactgcgtc gtatgctgcc gccgctgggt aaccagtgga
    31981 tcatcagcat taaagacacc tcgctgttta ttgtgatcgg cgtggcggaa ctgacccgtc
    32041 aggggcaaga aattattgcc ggtaacttcc gcgcccttga gatctggagc gccgtggcgg
    32101 tgttctatct gattattacc ctggtgctaa gctttatttt gcgtcgtctg gaaagaagga
    32161 tgaaaatcct gtgattgaat ttaaaaacgt ctccaagcac tttggcccaa cccaggtgct
    32221 tcacaatatc gatttgaaca ttgcccaggg cgaagtcgtg gtgattatcg ggccgtccgg
    32281 ttccggtaaa tcgaccctgc tgcgctgcat caacaaactg gaagaaatca cctccggcga
    32341 tctgattgtc gatggcctga aggttaacga tccgaaagtt gacgagcgcc tgattcgcca
    32401 ggaagcaggt atggtgttcc agcagtttta cctcttcccg catctgacgg cgctggaaaa
    32461 cgtcatgttt ggtccgctac gcgtgcgtgg cgcgaacaaa gaagaggcgg aaaaactggc
    32521 gcgtgagctg ctggcgaaag tcggtctggc agaacgtgca catcactacc cttccgaact
    32581 ttctggtggt caacagcagc gtgtggcgat tgcccgcgcg ctggcggtga agccgaaaat
    32641 gatgctgttt gatgaaccga cttccgctct tgacccggaa ctgcgccatg aagtgctgaa
    32701 ggttatgcag gatctggctg aagaagggat gacgatggtg atcgtgaccc acgaaatcgg
    32761 ttttgccgag aaagtagctt cgcggctgat ctttatcgac aaaggccgga ttgcggaaga
    32821 tggcgatccg caggtattga tcaagaaccc gccgagccag cgcttgcagg aatttttgca
    32881 gcacgtctct taacttctct gcctcccttt cacccgaaag ggaggcacac cagattcctc
    32941 tcatttaaaa tcgcccctcc cccagcatct atacttatct ttttgctctg ttttctcact
    33001 ggaggagtca tgcggtggat cctgttcatc ctcttctgcc tgctgggcgc acctgcccac
    33061 gcggtatcca tacccggcgt tacaaccaca acgacaacgg actcaacgac tgaaccggcc
    33121 ccggaaccgg atatcgaaca aaaaaaagcg gcctatggcg cactggcgga tgtgctggat
    33181 aacgacacct cgcgtaaaga attgatcgac cagttgcgta ctgtcgccgc gacgccccct
    33241 gcggaaccgg tcccgaagat cgtgccgccg acgctggttg aagagcaaac cgtgctgcaa
    33301 aaggtcaccg aagtcagccg ccattatggt gaagcccttt ccgcccgctt cgggcaactt
    33361 tatcgcaata tcaccggctc cccgcataag ccatttaatc cacaaacctt cagcaatgcg
    33421 ctgagccatt tttcaatgtt agcggtatta gtgtttggtt tttactggct gattcgcctg
    33481 tgcgcactgc cgctgtatcg caaaatgggt cagtgggcga ggcaaaaaaa tcgtgagcgc
    33541 agcaactggt tgcagcttcc ggcgatgatt atcggggcgt ttattatcga cctgctgtta
    33601 ctggccctga cattatttgt cggccaggta ttaagcgaca acctgaatgc aggcagtcgc
    33661 accatcgctt tccaacaaag tttgtttctc aacgcctttg ccctgattga atttttcaaa
    33721 gccgtactac gcctgatttt ttgcccaaac gtggcggagc tgcgcccgtt cacgattcag
    33781 gacgagagcg cccgttactg gagtcgtcgc ctgagctggt taagcagttt gataggttat
    33841 ggcctgattg tggccgtgcc gattatctct aatcaggtga atgtacagat aggtgcgctg
    33901 gcgaacgtca tcattatgct gtgcatgacc gtctgggcgt tgtacctgat ctttcgtaat
    33961 aaaaaagaga ttacccagca tttgctcaac tttgcggagc attcgctggc ctttttcagc
    34021 ctgtttatcc gcgcctttgc gctggtgtgg cactggctgg caagcgccta ttttatcgtg
    34081 ctgtttttct tttcgttgtt cgatccgggc aacagcctga aatttatgat gggtgcaacg
    34141 gtgcgcagcc tggcgattat tggtatcgca gcgtttgttt ccggtatgtt ttcccgctgg
    34201 ctggcaaaaa ccatcactct ctcgccacat actcagcgta actatccaga gttgcaaaaa
    34261 cggctgaatg gctggctttc ggcggcgctg aaaacggcgc gtattctgac cgtctgcgtg
    34321 gcggtaatgt tgctgttgag cgcatgggga ttgttcgatt tctggaactg gctgcaaaac
    34381 ggcgcggggc agaaaaccgt agatatcctg atccgtatcg cacttattct ttttttctcg
    34441 gcggttggct ggacggtgct cgccagtttg atcgaaaacc ggctggcttc ggatattcat
    34501 ggccgcccgc tacccagcgc ccgtacgcgt accctgctga cgctgttccg taacgcgctg
    34561 gcggtgatta tcagtaccat caccatcatg attgtgttgt cggaaatcgg cgtcaatatc
    34621 gcgccattgc tggcaggtgc cggggcatta ggtctggcta tctcgtttgg ttcgcaaacg
    34681 ctggtgaaag atatcattac cggggtgttt attcagtttg aaaacggcat gaacactgga
    34741 gatttggtga ctatcgggcc gttgaccggc acagtggaac ggatgtcgat tcgctccgtg
    34801 ggcgtgcgcc aggataccgg ggcgtatcac atcattccgt ggtcttcgat aaccaccttt
    34861 gctaacttcg tccgcggtat tggttcggta gtggcaaact acgatgttga tcgccatgaa
    34921 gatgctgata aagccaatca ggcactgaaa gatgcggtag cggaattaat ggaaaacgaa
    34981 gaaattcgcg ggctgattat tggtgaaccg aattttgccg ggattgtcgg cttaagcaat
    35041 accgcgttta cactgcgtgt ttcgttcacc acgctgccac tcaaacagtg gacggtacgc
    35101 tttgccctcg acagccaggt gaaaaaacat ttcgacctgg cgggcgttcg cgcgccagtg
    35161 cagacttatc aggtgctgcc tactccgggc gcgaccccgg ctgaaccgtt accgccgggg
    35221 gagccaacgc tttaacgctg gcgattgaca aaacggcggc gctgctcgtc gttcataaag
    35281 gtccaggcaa taaagcgact ctgcttttgc ccctgggcca tctctttttt taccaccttc
    35341 accgcgccca cgtcggtcag agcacgatac aacggcggta agttttcacc acgagatacc
    35401 agcgaggtaa accacatcac ctgcttcgcg aagcctttgc tctcttcaat catctttttg
    35461 ataaaggcga cttcaccacc ttcacaccac aactcctgtt gctgaccacc aaagttcagt
    35521 gcatcgtctt tgttcagccc caggttacgg cgtttacgct cactacctgc ccgcgctgca
    35581 gcggcggaat cgtggaatgg cgggttacac aaggtcgcgt cgtattgctc gtttttatgg
    35641 atgatgccgt taaaaattgc cccactctct ttttgccgac gcagacgaat ggcgcggtta
    35701 agccccggat tagcactgat aatcgcctgc gcactgctta acgcctggct gctggtttca
    35761 ctaccggtaa aacgccagcc atattcatgt acgccaatca gcggataaat acagttcgca
    35821 ccaacgccga tatccagaat gctggcattc gctggaattg ttccgcttgc ttcagccagt
    35881 aaatcggcaa ggtgatgaat ataatccgcc cggcccggta ctggtgggca gagaaaaccg
    35941 tcggggatat cccagttcgc tacggcgtaa aaatgggcca gcaacgcctt attgagcgcc
    36001 ttcaccgcca gcggattggc aaagtctacg ctttgctccc cggcgggtgt aagcgtgagg
    36061 aattgcctga gttcaggatt gacctgacaa agcgtggcga gatcgtagcg gctgtgatga
    36121 cggttgcgcg gatgcaaccc cggtttctgg gcggacatag cattctcctt taagcagcgg
    36181 cgtaagatac ccgcataacg cgacagggta aatatctggc tgcgtttatc attccccaaa
    36241 accttaacat ggctatggtt tatggcaatt catcataaaa atcagcatta tgcatgtcat
    36301 taatttgtct tattttgtaa aaatatatcg ttcaaaaaat aagcacatct ctgtcaattt
    36361 tttgcgtcat ttgcatcgcg caggcgacct acacttatca ggcactaccc gttgtattta
    36421 tgaggatgtc attatgaaaa agtgcctcac actactgatt gccaccgtcc tgagcggaat
    36481 ctctctcacg gcttatgccg cgcaaccgat gagtaacctg gacagcggtc agctacggcc
    36541 cgccggtacc gtttcggcaa ctggcgcatc aaacctaagc gatctggagg ataaactggc
    36601 agaaaaagcg cgcgaacaag gcgcgaaagg ttatgtcatt aattccgcag gcggaaatga
    36661 ccagatgttc ggtactgcaa ccatctacaa ataaccccac caacgtaact gcattcgcct
    36721 ctggatgcag ttttcgcctg ttttgtaatc aaaaaacatc aaatatgaac tcaatgtaaa
    36781 taaatgtatt tctttttcgc gcaatgggtg atagaaaatc gcgccaagtg ataatgctta
    36841 tcaaaattat tatcacttgc atgaacacta tcacgggatt aacagtggca tcgcatccgc
    36901 agagatgctt tctcgtggca gtgaaaattt caacatataa gaaaaagtca cctgcaaaat
    36961 ggaaaacaat cgcaatttcc ctgccagaca atttcattcg ctcacgttct ttgccggtct
    37021 ttgtattggc atcacgcctg tggctcaggc actcgccgcc gaagggcaaa ctaacgcgga
    37081 tgacacgctg gttgtcgaag catcaacgcc ttcgctttat gcgccacaac aatctgccga
    37141 tccgaaattc tcgcgtccgg tagcggatac tacccgcacg atgacggtga tttctgaaca
    37201 agtgattaaa gatcagggcg caaccaacct taccgacgcg ctcaaaaacg cccccggcg
//
LOCUS       DAJTVK010000029        36331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000029 DAJTVK010000000
VERSION     DAJTVK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.029"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="OO372_001501"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="OO372_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6341039.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="OO372_001502"
     CDS             1867..1992
                     /locus_tag="OO372_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341040.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="OO372_001503"
     CDS             complement(1989..2060)
                     /locus_tag="OO372_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341041.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="OO372_001504"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="OO372_001504"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6341042.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO372_001505"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="OO372_001505"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6341043.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="OO372_001506"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="OO372_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6341044.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO372_001507"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="OO372_001507"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6341045.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO372_001508"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="OO372_001508"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6341046.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO372_001509"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="OO372_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6341047.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="OO372_001510"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="OO372_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6341048.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="OO372_001511"
     CDS             complement(9465..9872)
                     /locus_tag="OO372_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR6341049.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO372_001512"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="OO372_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6341050.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO372_001513"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="OO372_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6341051.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="OO372_001514"
     CDS             complement(11538..12197)
                     /locus_tag="OO372_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6341052.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="OO372_001515"
     CDS             complement(12361..12672)
                     /locus_tag="OO372_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR6341053.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="OO372_001516"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="OO372_001516"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6341054.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="OO372_001517"
     CDS             complement(14207..14950)
                     /locus_tag="OO372_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6341055.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO372_001518"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="OO372_001518"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6341056.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO372_001519"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="OO372_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6341057.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO372_001520"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="OO372_001520"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6341058.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="OO372_001521"
     CDS             20005..20088
                     /locus_tag="OO372_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341059.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO372_001522"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="OO372_001522"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6341060.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO372_001523"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="OO372_001523"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6341061.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO372_001524"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="OO372_001524"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6341062.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO372_001525"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="OO372_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6341063.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="OO372_001526"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="OO372_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6341064.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="OO372_001527"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO372_001527"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="OO372_001528"
     CDS             25234..25836
                     /locus_tag="OO372_001528"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6341065.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="OO372_001529"
     CDS             complement(25937..25996)
                     /locus_tag="OO372_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6341066.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO372_001530"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="OO372_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6341067.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO372_001531"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="OO372_001531"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6341068.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO372_001532"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="OO372_001532"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6341069.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="OO372_001533"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="OO372_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6341070.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="OO372_001534"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="OO372_001534"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6341071.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="OO372_001535"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="OO372_001535"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6341072.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="OO372_001536"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="OO372_001536"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6341073.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="OO372_001537"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="OO372_001537"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCR6341074.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36331)
                     /locus_tag="OO372_001538"
     CDS             complement(35716..>36331)
                     /locus_tag="OO372_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6341075.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8703 a         9742 c         9170 g         8716 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataaccacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatcacaat t
//
LOCUS       DAJTVK010000030        35779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000030 DAJTVK010000000
VERSION     DAJTVK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.030"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..581)
                     /locus_tag="OO372_001539"
                     /pseudo
     CDS             complement(1..581)
                     /locus_tag="OO372_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO372_001540"
     CDS             complement(810..1730)
                     /gene="malM"
                     /locus_tag="OO372_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6341076.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO372_001541"
     CDS             complement(1882..3222)
                     /gene="lamB"
                     /locus_tag="OO372_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6341077.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO372_001542"
     CDS             complement(3294..4409)
                     /gene="malK"
                     /locus_tag="OO372_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6341078.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4774..5964
                     /gene="malE"
                     /locus_tag="OO372_001543"
     CDS             4774..5964
                     /gene="malE"
                     /locus_tag="OO372_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6341079.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            6118..7662
                     /gene="malF"
                     /locus_tag="OO372_001544"
     CDS             6118..7662
                     /gene="malF"
                     /locus_tag="OO372_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6341080.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7677..8567
                     /gene="malG"
                     /locus_tag="OO372_001545"
     CDS             7677..8567
                     /gene="malG"
                     /locus_tag="OO372_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6341081.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8939..10414
                     /gene="xylE"
                     /locus_tag="OO372_001546"
     CDS             8939..10414
                     /gene="xylE"
                     /locus_tag="OO372_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6341082.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO372_001547"
     CDS             complement(10458..10868)
                     /gene="psiE"
                     /locus_tag="OO372_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6341083.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10994..11137)
                     /locus_tag="OO372_001548"
     CDS             complement(10994..11137)
                     /locus_tag="OO372_001548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341084.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            11138..11416
                     /locus_tag="OO372_001549"
     CDS             11138..11416
                     /locus_tag="OO372_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341085.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11463..13559)
                     /locus_tag="OO372_001550"
     CDS             complement(11463..13559)
                     /locus_tag="OO372_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6341086.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13559..14296)
                     /locus_tag="OO372_001551"
     CDS             complement(13559..14296)
                     /locus_tag="OO372_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6341087.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(14293..14931)
                     /locus_tag="OO372_001552"
     CDS             complement(14293..14931)
                     /locus_tag="OO372_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6341088.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(15045..15287)
                     /locus_tag="OO372_001553"
     CDS             complement(15045..15287)
                     /locus_tag="OO372_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341089.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO372_001554"
     CDS             complement(15749..17398)
                     /gene="pgi"
                     /locus_tag="OO372_001554"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6341090.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            17923..19272
                     /gene="lysC"
                     /locus_tag="OO372_001555"
     CDS             17923..19272
                     /gene="lysC"
                     /locus_tag="OO372_001555"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6341091.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19524..19796
                     /locus_tag="OO372_001556"
     CDS             19524..19796
                     /locus_tag="OO372_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6341092.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO372_001557"
     CDS             complement(19929..20801)
                     /gene="rluF"
                     /locus_tag="OO372_001557"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6341093.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            21149..21986
                     /locus_tag="OO372_001558"
                     /pseudo
     CDS             21149..21986
                     /locus_tag="OO372_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            22056..22862
                     /locus_tag="OO372_001559"
     CDS             22056..22862
                     /locus_tag="OO372_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6341094.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22872..23279
                     /locus_tag="OO372_001560"
     CDS             22872..23279
                     /locus_tag="OO372_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6341095.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            23279..23773
                     /locus_tag="OO372_001561"
                     /pseudo
     CDS             23279..23773
                     /locus_tag="OO372_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23839..24636
                     /locus_tag="OO372_001562"
     CDS             23839..24636
                     /locus_tag="OO372_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6341096.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMAQVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24647..25471
                     /locus_tag="OO372_001563"
     CDS             24647..25471
                     /locus_tag="OO372_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6341097.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25523..26751
                     /gene="sorE"
                     /locus_tag="OO372_001564"
                     /pseudo
     CDS             25523..26751
                     /gene="sorE"
                     /locus_tag="OO372_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            27134..27823
                     /gene="pepE"
                     /locus_tag="OO372_001565"
     CDS             27134..27823
                     /gene="pepE"
                     /locus_tag="OO372_001565"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6341098.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27914..29545)
                     /locus_tag="OO372_001566"
     CDS             complement(27914..29545)
                     /locus_tag="OO372_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6341099.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO372_001567"
     CDS             complement(29765..33448)
                     /gene="metH"
                     /locus_tag="OO372_001567"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6341100.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33648..34472
                     /gene="iclR"
                     /locus_tag="OO372_001568"
     CDS             33648..34472
                     /gene="iclR"
                     /locus_tag="OO372_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6341101.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            34789..35779
                     /gene="espL4"
                     /locus_tag="OO372_001569"
                     /pseudo
     CDS             34789..35779
                     /gene="espL4"
                     /locus_tag="OO372_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT         8599 a         9231 c         9100 g         8849 t
ORIGIN      
        1 actatgacca agttgactct gcgagaagtc aactgtatca agaatatttt ggctaaaatc
       61 aacacattcg ccactcgttt tgagaaaaat cacattgtgt aaacgtcgac atttaaatat
      121 gacattatca actgttgcct tagtaaaatt gcaattctca atttctttac attcgaaagt
      181 tacatgttgt agatttgatg ataaaaaaga caccttagaa ataacgtcac ctttaaaatg
      241 acaatttatt aaatcaaaat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      301 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      361 actaattgag acttcacgta acatatttat tgcatgattt tttcgttccg ttgctttata
      421 tttaaaaata tatcactgat ataaataaag ttatctctgt ttaaacctgt caccccattg
      481 aaattacgta gtaaatcaag atctactata ttatttcctt gaaccacatc atgtttcatc
      541 aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct gtaatacaga
      601 aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa aggaaaagaa
      661 tctctgataa ggcattgaga taatggacat tcttagtgaa gaagaagtga agtactataa
      721 aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt
      781 tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact gctgataaag
      841 gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt taacgcctta
      901 tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact ttccgtgtcg
      961 ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac agctggtgcc
     1021 gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagtgg tcctaccaac
     1081 acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct ggtatgacga
     1141 gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata ggctttagcc
     1201 gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac cagaacataa
     1261 agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg atctgcactc
     1321 atcacgcctg gttcctggta ggtgaaataa ctgctgggga agaaggctga tggggtcatg
     1381 ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg
     1441 gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc aaccggacca
     1501 ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt ctgggtttta
     1561 gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg
     1621 tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg cgcgcttgcc
     1681 agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat tgttttcacc
     1741 tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat aatcagaggg
     1801 gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc gtaaacgcct
     1861 tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga aggtccactc
     1921 gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg ctttgccgta
     1981 gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat cccacttggc
     2041 gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag cctgccattg
     2101 ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg attcgacgtt
     2161 gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca tcgggcgaat
     2221 accgacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct ggtacatacc
     2281 cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag
     2341 catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg cgacgccaga
     2401 accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact gaacaacaaa
     2461 cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc agccgtcttt
     2521 cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt agtcgacacc
     2581 cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat cgaaaacgtc
     2641 gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg aagaaccacc
     2701 agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat cgatgttttc
     2761 cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat gaacgtcatg
     2821 acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt cgatcaggtt
     2881 tttaccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt cattctgttg
     2941 cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct ctttccacac
     3001 ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac ggtatttact
     3061 ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc agccaatacc
     3121 ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta cgcccgctgc
     3181 gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc ctgagtcatt
     3241 gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg
     3301 cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag atggcaacgc
     3361 tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac gtcgttctgg
     3421 cggtacacca ggttttgacg aatggaaggg atctggatat ggatttgggt ttcgttgccg
     3481 agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc actcggcagt
     3541 agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc acggctttca
     3601 actggcagcc agacttgctg acgatttggc atcggcagct ccacctgcac ttgatcgatt
     3661 gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat aaatccggcg
     3721 acaaaacggt ctgccggata gtggtacagc tccagcggtt tcccaacctg cgccacgcga
     3781 ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg
     3841 acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat acgcatttgc
     3901 acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac gcttggctcg
     3961 gccaccagcg tacggccaat cgccacacgc tgacgctgac caccggagag cgctttcggt
     4021 ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac gcgttggtta
     4081 atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc tgctactgac
     4141 aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc tgctggcgga
     4201 gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg
     4261 gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa caccacgaat
     4321 tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc ccaggctttc
     4381 gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa cctgtcatcg
     4441 acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa atcctccacc
     4501 ccctggcttt tttatggggg aggaggcggg aggatgagaa cacggcttct gtgaactaaa
     4561 ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc
     4621 gcatctccac attaccgcca attctgtaac agagatcaca caaagcaacg gtggggcgta
     4681 ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt ttaggtgttt
     4741 tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg tgcacgcatc
     4801 ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa
     4861 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     4921 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     4981 gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat cttctgggcg
     5041 cacgaccgtt ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5101 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5161 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5221 aacccaccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5281 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5341 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg atattaaaga cgtggccgtg
     5401 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg accttattaa aaacaaacac
     5461 atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5521 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5581 gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc
     5641 gccggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct cgaaaactat
     5701 ctgctgactg acgaaggtct ggaagcggtc aataaagaca aaccgctggg tgcagtggcg
     5761 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     5821 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     5881 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     5941 gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt
     6001 ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc
     6061 agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg
     6121 gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt
     6181 ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg ggaatacctg
     6241 ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc caatcgtaaa
     6301 gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt cgtcctcttc
     6361 cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa ccagctgact
     6421 tttgaatgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa aacctataac
     6481 tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagtgacgg cgaaatcggc
     6541 aaaaattacc tctccgacgc gtttaaattt ggcggcgaac aaaaactgca actgaaagaa
     6601 acgaccgccc agcccgaagg cgaacgcgcg aatctacgcg tgattaccca gaatcgtcag
     6661 gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat gagctccctg
     6721 cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac gttgacgaat
     6781 aatcagagcg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca gtccattacc
     6841 gccgacggca actggggtga tgaaaagcta agccccggtt acaccgtgac tactggctgg
     6901 aaaaacttta cccgcgtctt caccgacgaa ggcattcaga aaccgttcct cgccattttc
     6961 gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt cggcatggtt
     7021 ctggcatgtc tggtgcagtg ggaagcattg cgcggcaaag cggtctatcg cgttctgctg
     7081 attctgccct acgcggtgcc atcattcatt tcaatcttga ttttcaaagg gttgtttaac
     7141 cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg
     7201 ttcagcgatc cgaccaccgc ccgcacgatg ctaattatcg tcaatacctg gctgggttat
     7261 ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga tttgtatgaa
     7321 gcctcagcaa tggatggcgc agatccgttc cagaacttct ttaagattac gctgccgctg
     7381 ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt taacaacttc
     7441 gtgctgattc aactgttaac caacggcggc ccggatcgtc ttggcacgac cacgccagcc
     7501 ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg cggcgggggt
     7561 caggacttcg gtctggctgc agcaattgcc acgctgctct tcctgctggt gggtgcgctg
     7621 gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata acaaaaatgg
     7681 caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg ctactgctac
     7741 tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctca ctgcgccagg
     7801 ggaactttgc gatcggcagc ctgatcccgg agcaaatctc ctgggatcac tggaaactgg
     7861 ctttaggttt tagcgtggaa caggctgatg gtcgcattac gccaccgcca ttcccggtac
     7921 tgctgtggct atggaactcg gtaaaggtcg ccgggatttc cgcgattggc attgtcgcgc
     7981 tctctaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc
     8041 tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc gcgttgtatg
     8101 cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact cacggcggcg
     8161 taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg
     8221 aaaccatcga tagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg
     8281 ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta ttcatcctgt
     8341 cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca
     8401 gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac ctgtggggtg
     8461 actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc ttgctggctc
     8521 aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg
     8581 ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact gttattcggc
     8641 gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt
     8701 tccatcatag agcttattat ttttacgtta tttgttttcc cacttacgat aattctcttt
     8761 cgtgctctga gtcacggcaa tagtattgtt ttcatcaatt tttgataatt atcacaatta
     8821 agatcacaga aaagacatta cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc
     8881 tgacaattcc aacatcaatg cactgataaa agatcagaat ggtctaaggc aggtctgaat
     8941 gaatacccag tataattcca gttatatatt ttcgattacc ttagtcgcta cattaggtgg
     9001 tttattattt ggctacgaca ccgccgttat ttccggtact gttgagtcac tcaataccgt
     9061 ctttgttgct ccacaaaact taagtgaatc cgctgccaac tccctgttag gattttgcgt
     9121 agccagcgct ctgattggtt gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg
     9181 cttcggccgt cgtgattcac ttaagattgc tgctgtcctg ttttttattt ctggtgtagg
     9241 ttctgcctgg ccagaacttg gttttacctc tataaacccg gacaacacag tacctattta
     9301 tctggcaggt tatgtcccgg aatttgttat ttatcgtatt attggcggta ttggcgttgg
     9361 tttagcctca atgctttcac caatgtatat tgcggaactg gctccagctc atattcgcgg
     9421 aaaactggtt tcatttaacc agtttgcgat tattttcggg caacttttag tttactgcgt
     9481 aaactatttt attgcccgtt ccggtgatgc cagctggctg aatactgacg gctggcgtta
     9541 tatgtttgcc tcggaatgta tccctgcact gctgttctta atgctgctgt ataccgtgcc
     9601 agaaagtcct cgctggctga tgtcacgcgg caagcaagaa caggcggaaa gtatcctgcg
     9661 caaaattatg ggcaacacgc ttgcaactca ggcagtacag gaaattaaac actccctgga
     9721 tcatggccgc aaaaccggtg gtcgtctgct gatgtttggc gtgggcgtga ttgtcatcgg
     9781 cgttatgctc tccatcttcc agcaatttgt cggcatcaat gtggtgctgt actacgcgcc
     9841 ggaagtgttc aaaacgctgg gggccagcac ggatatcgcg ctgttgcaga ccattattgt
     9901 cggagttatc aacctcacct tcaccgttct ggcaattatg acggtggata aatttggtcg
     9961 taagccactg caaattatcg gcgcactcgg aatggcaatc ggtatgttta gcctcggtac
    10021 cgcgttttac actcaggcac cgggtattgt ggcgctactg tcgatgctgt tctatgttgc
    10081 tgcctttgcc atgtcctggg gtccggtatg ctgggtaccg ctgtcggaaa tcttcccgaa
    10141 tgctattcgt ggtaaagcgc tggcaatcgc ggtggcggcc cagtggctgg cgaactactt
    10201 cgtctcctgg accttcccga tgatggacaa aaactcctgg ctggtggccc atttccacaa
    10261 cggtttctcc tactggattt acgggtgtat gggcgttctg gcagcactat ttatgtggaa
    10321 atttgtcccg gaaaccaaag gtaaaaccct tgaggagctg gaagccctct gggaaccgga
    10381 aacgaagaaa acacaacaaa ctgctacgct gtaatcttcc tgtccagcac gccgcgccag
    10441 ttcggcgtgc tttcttttta ctcccgcttc agccgtttcg aattacacag ccacagagtg
    10501 atcaccagta acaggatcgc agccgagtag atcaacacat ccagtggcga tttatgatcg
    10561 acgatgatca agcgcacaat ggcggtgatc ccaatataga caaagtaacg tagcgggaag
    10621 tgaaaaccgg actgaaagta cttcacaatc agcgcgataa attcgaaata gagaaagtaa
    10681 accaccagtc cttctaccag ctcatatttg ctggtttgtt ctggcgcgaa cagcacatct
    10741 gccagatgca ccgtttcttt gccgaggaag acaaccaaaa tcaggccgag gcacagcagg
    10801 ccaagattga gtacggtctg caaaatggtg gagataaact ccacgcgcgg acgagagagt
    10861 gacgtcatag cagtacctcc ttcacagtgg cgagcttcct gtataacgca aaaatgtgac
    10921 ggagatctat attttttgtg tatgttttgt tcaacctgga tttggagcgc gcctccacca
    10981 aagccggata tacctacccg taggccggat aagccgcgcc tggcggcgca tccggcaaga
    11041 caaaccgcac ccaagccggt aattcaaaaa tcacccattc aggtgattcc cagccaccag
    11101 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    11161 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    11221 tgctggtccg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    11281 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    11341 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    11401 ctcaacaggc cattaagaat gagggtgcca gtgattgcga aaactggcac ctttcccatg
    11461 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    11521 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    11581 gggccagacg agaagagatc caacggtacc gagacataca cgcctttggt gaagtcccct
    11641 tcgccgtact cctcttttga aacattcgtg atcgtggcat agccacccac caccacgccg
    11701 ctatcaaagc gtttggcgat ctccagcgtg ccgcctttat cccctgccag atactgcccg
    11761 acgctggctt tcactaacac gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt
    11821 ccggttttca cgctgtagtc ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc
    11881 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tgtccagcgg acgatacaac
    11941 acttcagccc ccgcaccgcc aaacatggtt tcgagataac ctccatagac ctgaccgtag
    12001 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata ggcatcgttc
    12061 tgcacatact cgcgcacatg ggtacgaacg cgcggtaagt gcgagtcctg tggcggattg
    12121 gtgtagttaa acttgtcgta gttgttggca agatttgcaa acaggctgcc gatggtcagc
    12181 agatggtccg tcagccacaa atctgccgtt cccatcacgc ccagctgata catgtaaaag
    12241 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    12301 ttgtcgatat accagccctg ctcggtggac tgcggaacca ctggctcgac gcgtttttgc
    12361 gccagcgtcg tttcgtggcc caacggctct ccggcgagat gatttttcag gctggcgaca
    12421 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    12481 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    12541 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    12601 ggatcggcaa gtccggcatt gtatttcagc aacgttaact gattcgccac caccgagtgc
    12661 tgcaaaatgg catcctgcgg ctgcggttga tattgcgggc gggcgttatc gatgtaggac
    12721 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    12781 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    12841 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    12901 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    12961 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    13021 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    13081 tatccccagc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    13141 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    13201 agccagtaac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    13261 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtaacgc
    13321 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    13381 ttatcgcgat agttcagact caactcccct tcccgtgcca tacgcgcggt gggagtttgt
    13441 aataatccta cgccaccgaa atccgactgc gacggaccaa tgggcgcagg atatgtttcg
    13501 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    13561 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggcgtct
    13621 cactccagac ggagtctgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    13681 gacgcttgtt ccagtaagcc accggcgctt tttgcgtgcg tccgtccggg taaacgaccc
    13741 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    13801 cgcgacctgg agtgaatggc tgattgccag gatggctgat aagcccgaac aacgtgaccg
    13861 tggacggcgg tggtccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    13921 cggcaacgcg gacgatatcg ggatcaagat tgattttttg cctgcctgtc acttccaatg
    13981 cctggatttg ctggcgtaag gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc
    14041 cctgttctgc cagccgtgtc agcaacgcct gctgctgacg caatgctgcc gccgttgcca
    14101 gctcttcgct aatcaccgca ccaggccacc agctgtttgc cagtcgaggt tgccctacca
    14161 gatcgattaa atgctctgcg cccgttagcg ttttggcctc actgctgcca ttgctgaaca
    14221 ccttgacggt tcctgccgcg aagaccgatg acgctcccac actcaaaagc aacgcgacaa
    14281 tagtttgttt aatcattgtt ttgccgcctt gatgagagtg gttttcaccg ggaaataatc
    14341 cgcgccgaga tactgttccg actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt
    14401 atgccagcgc gcctggtcgg tggagacttc ttcgtccagc acgcgaacca gggtttcatc
    14461 gctgccgacc ttgacggtat cggtgccatc ccatttgaag actgagcgtg cggtggcgta
    14521 gcgtacctgc tggtactcgg tccagcccat cgtgcgcgtc catgttgcac catcaacaat
    14581 ttgtgcgggt ttaatcagcg ggtcggcggc aaggttattc acttcaatca ggttgtcgcc
    14641 gccaagcaaa gtcttcacca gacgaccatg ttgtgtgaca agagtggctt gatcctgagt
    14701 gacccatttt tgttgtccgt cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc
    14761 attaagctgc atgtactggc tggcgtaggg catattttga atatcatcgt ccgtcagctg
    14821 tacgcctggc gtgccgaaca gactgtccca cagtgaattg cccagctctt tagtcgtggc
    14881 tgaacaggcc tgtaataaca ggcagataag aatgagtgca ggtcgcttca cgactcttct
    14941 ccagaggggc agaataacca caccggagtg tggttatggt taatcccttc atatttcaag
    15001 ccacagatgc aatgactcga attattttgg ggatacatac tttattactg ggtactggtg
    15061 gttgtggttg ttgtggtccc ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg
    15121 acacccaccg ccgagcttac ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc
    15181 gacgttgctg ccgcccctgc cgcttcgccc acctgtacag gtgcagccca cacagaagtc
    15241 gccgcaagcg cagatatggc aaaaatgcca tacagaactt ttttcataac aatttccctt
    15301 cattgaatga atggagattt acccaaaaac atttcgggcg ggatcgagta tacacaacta
    15361 aagcatgcga attacggcgc ggggaattag cgatggtgtt ttaggataat tggaatgaat
    15421 atcaaatata agtttcaact tatatttaaa taacaataaa aatcataaac ttaaaataaa
    15481 aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa tctattttag
    15541 gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat ctgataaaac
    15601 aatgccgact tattgtcggc attgttttta aggttattta actcctcata ctatgcatta
    15661 atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct tatccggcct
    15721 acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag cggttaatca
    15781 gaccattggt cgagctatcg tggctgctga tttctttgtc atctttcagc tctggcagaa
    15841 tacggttcgc cagttgttta cccaactcca cgccccactg gtcgaaggta aagatgttca
    15901 ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca cccaggctga
    15961 acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg aacactttga
    16021 acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat tcctgctcaa
    16081 ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag ttagacagca
    16141 gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg aaatcgcacg
    16201 gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg
    16261 gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta cggtcaacat
    16321 acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact
    16381 ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attattgtac cagatgccaa
    16441 tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca
    16501 tcgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag agaacaatcg
    16561 acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag aactcgaaca
    16621 tgttggcagt atcaatacca aactcgccaa cggctttggc attggtggaa agcgccgcaa
    16681 agtgttttgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca cgcgcgctat
    16741 gggcgttggt catagtttcc tgagtggtga aggttttaga tgctaccagg aacagcgtgg
    16801 tttccgggtt tacttttttc agcacttcag cgatgtgagt cccatcgacg ttagaaacaa
    16861 agtgcatgtt caggtgattt ttgtacggac gcagtgcttc ggtcaccatg tacgggccga
    16921 ggtcagaacc accgatcccg atgttcacta cgtcagtgat tgctttgccg gtataaccct
    16981 tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc accgcgttga
    17041 cttccggcat tacgtctttg ccatcaacca gaatcggggt attgctacgg ttacgcagcg
    17101 ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag aacatcgact
    17161 taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc agcgtctctt
    17221 cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaatgtt gcggagaact
    17281 tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat
    17341 cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg tttttcatta
    17401 gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc agtcacagaa
    17461 aaatgtgatg gttttagtgc cgttagcgta atgttgagtg taaaccctta gcgcagtgaa
    17521 gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca tgaccccgtc
    17581 gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct agcgcaggcc
    17641 agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga taacacctga
    17701 gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc tacaggggct
    17761 gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag tggtggagca
    17821 cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc
    17881 ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga aattgttgtc
    17941 tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag cgctgatatt
    18001 gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg tatcactaat
    18061 ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa actcgacgct
    18121 attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa
    18181 gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc gctggcaacg
    18241 tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac cctgctgttt
    18301 gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg taaagtgatg
    18361 cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc
    18421 gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg atttatcggt
    18481 agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta tacggcagcc
    18541 ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt cccgggcatc
    18601 tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc
    18661 gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac gttgctaccc
    18721 gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg cgcaggtggt
    18781 acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc
    18841 aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg tttcctcgcg
    18901 gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac cacgtcagaa
    18961 gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga tacgttgctg
    19021 acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaagtaga agaaggtctg
    19081 gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg caaagaggta
    19141 ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc cagccataac
    19201 ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact gcatagtaat
    19261 ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt gattttcttg
    19321 tcactatgct tatcaataaa cgagcctgta ctctgttaac caacgtcttt atcgagaata
    19381 attgccttta atttttttat ctgcatctct aattaattat cgaaagagat aaatagttaa
    19441 gagaaggcaa aatgaatatt atcagttctg ctcgcaaacg aattcccgta cactgaactc
    19501 tctggctcac tgagggcacc atcatggcac tcccccgtat tacccaaaaa gagatgactg
    19561 aacgcgagca gcgtgaactt aaaacactgc tggatcgggc gcggattgcg catggtcgcg
    19621 tactgaccaa ctccgaaacc aacagcatta agaaagagta catcgataaa ttaatggtcg
    19681 agcgtgaggc tgaagcgaaa aaagcccgcc aattgaagaa aaagcaggct tataaaccgg
    19741 atcctgaagc gtcatttagc tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa
    19801 caatcaacgc ctgagatttt cactcgtctg tgtgaacaca attttcccct caccctaacc
    19861 cttttcccga aggggcgagg ggactgtccg agcacttttg ggccgttttc atcagtctga
    19921 ggcgttaatc agcgcccctt ctttttacgc cccggcgagg taaaacgctt accgttggac
    19981 gccgggcgac cgcctttttc cgccgttttt tccatcttaa cgactggacg tttgatgccc
    20041 gctgttttcg gtttggcctt cgctttgggc ttcgcctcgg aagaggaatt ttcaatgagc
    20101 ttaaagagat cgataagctc gtcgtcggtt aaatcgcgcc attctcccag cggaatgccg
    20161 cttaagctga cgttcataat gcgcgtacgt tccagctttt tcacttcata gccgaaatgc
    20221 tcgcacatgc gccggatctg acggttcaac ccctgcacca gggtaatgcg gaagacaaac
    20281 ggtgcttctt ttttgacttt gcactttttc gtcaccgtcc cgaggatcgg cactcccgcc
    20341 cccataccac gaataaattc atcggtaatc ggtttatcga ccgtcaccag atactctttc
    20401 tcatgatcgt tacccgcacg gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa
    20461 atcagcccct gggagtcttt atccaggcgg ccaatcggga acacgcgttt gctgtggtta
    20521 acgaaatcga caatgttatc gcgctcgcca tcttcggtgg tgctgacaat accgacgggc
    20581 ttgttcaggg cgataagtac caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt
    20641 actttcacaa tgtcgccggg tttcacctga tcaccaatgg tggctcgctt gccattaagg
    20701 aacacattgc cttgctcgat atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg
    20761 ctgatgtatt tatttaaacg gactgatgag tcgggcagca taaattctcc tgtaaaacgc
    20821 ggaatatacc gtacctctta gcggggaaaa acagggttat gcggcaacgt cgaaattaaa
    20881 tgtgatctgc cacgcctttt atcacaaaag tgtctccgga agtacgatgc aaatttgcct
    20941 ggcaaaataa ggataatcaa catattacga atcgctgcac acctgtgctg atgactacta
    21001 cgcagagaaa caaatgtgca agagaaagcg ctatttctcc ccatgcaaag agtgaaaaat
    21061 cgctttgaaa ggccctcata atggggttat atcagatcaa gcggtgagaa agcgtatttg
    21121 cacaaatgtg cagaaccagg aggcggcaat ggaaaacagt gacgatatcc gtttgattgt
    21181 gaagattgcc caactctatt acgaacagga tatgacgcag gcgcaaatcg cgcgcgaact
    21241 gggtatttac cgcaccacca tcagccgctt gcttaaacga ggccgcgatc agggaattgt
    21301 caccatcgcc atcaactatg actacaacga aaatctctgg ctggagcagc aactgaagca
    21361 aaagtttggc ttgaaagacg ttgtggtggt gtcgggaaat gatgaggatg aagagactca
    21421 actggcgatg atggggttac acggcgcgca actgctggat cgcttgctgg agcctggcga
    21481 tattgtcggt ttttcctagg gtcgcgcggt gagcgcactg gttgaaaact tgccgcaagc
    21541 ggggcaatcg cggcagttaa tctgcgtgcc aattattggc ggaccgtccg gtaaactcga
    21601 aagccgctat cacgtaaaca cattaaccta cagcgtggca gcgaagctga aaggggaatc
    21661 gcatctcgcg gattttccgg ctctgctgga taacccctta attcgtaatg ggatcatgca
    21721 gtctcagcac tttaaaacca tctctgccta ctgggataat ctggatgtcg ccctggtggg
    21781 aattggctca ccggccattc gcgacggcgc taactggcat gcgttttatg gtggtgaaga
    21841 gagtgacgac ctgaaagcag gcgcggtatt ccattggcat tgccatgagt gaagaaaaat
    21901 acagcggaat tgttggtgca ctgcgtggaa aatatattaa ttgtctggta acgaatagca
    21961 gcacagctga actattactg aaataaaaat cttcccgatg tttaccgtaa taaaaattac
    22021 ggcgggtccc tattatctaa aatcaggagt ctgttatgca aacgtggtta aatttgcagg
    22081 ataaaatcat tatcgtcacc ggcggcgcat ccggtattgg tctggcgatt gtagaggaat
    22141 tattagcaca aggcgcaaac gtacagatgg tagatattca cggtggcgat ggtcaatatg
    22201 aagttcataa aggctatcag ttttggccga ccgatatttc cagcgccaaa gaggtaaatc
    22261 atacggtagc ggaaattatc cagcgttttg gtcgcatcga cggtctggtc aataacgccg
    22321 gggtcaattt cccgcgtctg ctggtcgatg agaaagcgcc tgccgggcag tatgaactca
    22381 acgaagctgc attcgaaaaa atggtcaata tcaaccaaaa aggcgttttt ctgatgtcgc
    22441 aggcggtggc gcgccagatg gtcaaacaac atgatggcgt gattgtgaat gtttcctcag
    22501 aaagtggact ggaaggctca gaaggccaaa gctgttacgc cgctaccaaa gccgcgctca
    22561 atagcttcac gcgctcctgg tcgaaagagc tgggtaaaca cggtatccgt gtggtcggta
    22621 tcgcgccggg gattctggaa aaaaccgggc tacgcacgcc ggaatatgaa gaggcgctgg
    22681 cgtggacgcg caatatcacc gtcgagcagc tgcgtgaagg ctataccaaa aacgccattc
    22741 ctattgggcg cgccggaaga ttagcagaag tcgctgattt tgtttgttat ctgctgtctg
    22801 aacgcgccag ctacatcacc ggagtaacca ctaacattgc gggcggcaaa acgcgcggat
    22861 aaggaggctt tatggtcaac gccatttttt gtgcccacgg caaattagct tgcgccatgc
    22921 tggaatcggt tcagatggtg tacggcgacg ccaatgtcga ggcggttgaa tttgtaccag
    22981 gcgagaacgc cggagacatc gtagcaaagc tggaaaagtt agtaagcatt cacaatcaag
    23041 atgagtggct gattgccgtg gatttacagt gcggcagccc gtggaatgcg gcagcgatgc
    23101 ttgcgatgcg caatcctcgt ctacgggtaa tcagcggcct ttccctgccg ctggcgctcg
    23161 aactggttga taaccaggac agcatgaatg ttgatgaact gtgtgaacac ctgacgcaaa
    23221 tcgcaaagca aacctgcgtt gtctggaaac aactggcaac ggcagaggaa gatttctgat
    23281 gaacattacg ctcgcccgca ttgatgaccg tctgatccac ggccaggtca ctaccgtctg
    23341 gtcgaaagtg gctaacgccc agcgcattat tatctgcaat gacgaagttt ataacgatga
    23401 agtgcgccgg acattattgc gtcaggctgc gccaccggga atgaaggtta acgtggtcaa
    23461 tattgaaaaa gccgtcgcgg tttatcacaa cccgcaatat taggacgaaa ccgtttttta
    23521 tcttttcact cgcccacagg atgccttagc gatggttcgc cagggggtaa aaataggcac
    23581 cctgaatatt ggcggcatgg cctggcggcc cggtaaaaaa cagttaacca aagcggtttc
    23641 attagatgat gatgatatta acgcatttca tgagttaaat aatcttggcg tcataatgga
    23701 tttacgcgtc gtagcgtcag atccctcaat caatattatc gacaaaataa acgaacagtt
    23761 aatcgcaaat taaataatag cgcctgtgta catatgcttt aacaggcagg gatgacgaca
    23821 cttaaaaggt gacaaatcat ggaaataagt accctacaaa ttattgctat atttcttttt
    23881 tcctgtattg ccggaatggg cagcgtgctg gatgaatttc agactcatcg cccgttaatt
    23941 gcctgtacgg tgattggttt aattctcggt gatttaaaaa ccggaataat actcggtggt
    24001 acgctggaat tgatagctct cggctggatg aacgtcggcg cggcgcaatc tccggattct
    24061 gcactcgcca gcataatctc cgccattctg gttatcgttg gtcagcaaag catcgccacc
    24121 ggaatcgcca tcgcgttgcc tgtggctgcg gcaggccaag tgctgacggt gtttgcccgt
    24181 accatcaccg tagtgttcca gcacgcggcg gataaagcag cggaagaggc gcggtttcgc
    24241 actctcgata ttctgcatgt ctccgcgctt ggcgtgcagg cgctgcgcgt tgctattccg
    24301 gcactgattg tctcactgtt cgtcagcgcc gatatggtca gcaatatgtt aaacgccatt
    24361 cccgaatttg tgacccgtgg actgcaaatt gctggcggtt ttatcgtggt ggtcggttac
    24421 gccatggtgc tacgtatgat gggcgtgaaa tatttgatgc ctttcttttt cctcggtttc
    24481 ctcgcaggtg gctacctcga tctcagcctg ctggcgttcg gtggcgtcgg cgtgatcatg
    24541 gcccaggtct acatccagct aaatccacag tggcgtaaag ctgaacctca actccaggcc
    24601 accgcctcca ccgcccttga ccaacttgac gattaacgga gccatcatgg aacagagaaa
    24661 aattacacgc agcgatctgg tgagcatgtt tctgcgctcc aacctgcaac aggcgtcctt
    24721 taactttgaa cgtattcacg gactgggctt ttgctacgac atgatccccg ccatcaagcg
    24781 actttaccca ttaaaagagg atcaggttgc ggcgctcagg cgacacctgg tgttcttcaa
    24841 taccacgcca gccgtatgtg gcccggtcat cggcgtcact gccgccatgg aagaggcgcg
    24901 ggccaacggc gcggaaattg atgacggtac cattaacggc atcaaagtcg gtctgatggg
    24961 accattggca ggagttggcg atccactggt ctggggaacg ctgcgcccga ttaccgctgc
    25021 gctcggcgca tctctggcac tttctggcaa catcctcggc ccgctgctgt ttttctttat
    25081 tttcaatgcg gtgcgtctgg cgatgaagtg gtatggccta cagctcggct ttcgcaaagg
    25141 ggtgaatatc gtcagcgata tgggcgggaa tgtgctgcaa aaactcaccg aaggcgcgtc
    25201 gattctcgga ctgtttgtga tgggcgtgct ggtcaccaaa tggacgtcaa tcaacgtacc
    25261 gttggtggtt tcacaaacgc atgccgccga tggctccacc gtcatcatga ccgtgcagaa
    25321 cattctcgac caactttgcc ctggtttgct ggcgctcggt ctgacgctac tgatggttcg
    25381 tctgctcaac aaaaaaatta acccggtatg gctgattttc gccctgtttg gcttagggat
    25441 tatcggcaat gcgctgggct tcctgtcctg attcttcgcc ccggcacgac tgccggggcc
    25501 atcgctcaac atgaggtggt ttatgaaaac aacagctctg cgtctttatg gtaaacgtga
    25561 tttacgcctg gaaacctttg accttcctga aatgcaggag gatgaaatcc tcgcgacggt
    25621 ggtcactgac agcctgtgcc tctcttcctg gaaagaggca acctgggtga aaaccataaa
    25681 aaagtacccg acgatgtggc gaccaaccca atcatcatcg gccacgagtt ttgcggcgat
    25741 attctggccg tgggtaaaaa gtggcagcac aaattccagc cgggtcagcg ttatgtgatt
    25801 caggccaacc tgaaactccc tgaccgcccg gactgccccg gctactcctt cccgtgggtg
    25861 ggcggcgaag ccacgcatgt ggttattccc aacgaggtca tggaacaaga ttgcctgctg
    25921 gcatacgacg gcgaaaccta ttttgaaggc tcgctggttg aaccgctttc ctgcgtgatt
    25981 ggcgcgttca acgccaacta tcatcttcag gaaggtagtt ataaccacac gatggggatt
    26041 cgcccgcaag ggcgcacgct gatcctcggc ggcaccggac caatgggatt gttggcgatt
    26101 gattatgcgc tgcatggacc agttaacccg tcgctgctcg tcattaccga taccgacaac
    26161 gacaaattga gttatgcgcg caagcactat ccatcagaac cgcaaacact gattcattat
    26221 ctcaatgcca ccgatgcagc atttgatacg ttaatggcgc tgagtggcgg tcacggcttc
    26281 gatgatattt tcgtctttgt gcctaatgaa ggactggtga ctctcgcctc ttccttgctg
    26341 gcgacagatg gttgcctgaa tttcttcgcc ggaccgcagg ataaacattt cagcgcgcca
    26401 attaatttct acgatgtgca ttatgcattt acccactacg tgggcacgtc aggcggcaat
    26461 accgacgaca tgcgcgcggc ggtcaaactg attgaagaga aaaaagtgca ggccgcaaaa
    26521 gtggtaacac atattcttgg gctgaatgcc gcgggcgaaa ccacgcttga attgcctgcc
    26581 gtcggcggcg gcaaaaagct ggtgtatacc gggaaatacc tgccgctgac gtcactcacg
    26641 cagattcagg atcaagcact ggcggcgatt ctggcgcgtc atcaggggat ctggtccggt
    26701 gaggcggagc aatatctgct cactcatgca gaggcaattt cccatgatta atcgcgctac
    26761 ttaactctgc atgtcgctgg ctggtcgccg cggcaacttc gcgacctgat gtgcggctga
    26821 ttgctgaagc ggcccaatgt gcacgagaag gataaaaaaa catcagcgcc ttaaggggcg
    26881 ctcaaattgg tggcaaagta cacattgttt atgccggatg cggaataaag ccttattcgg
    26941 cctacataat ctcgaaaatt caatatattg cagttatctg gtaggcctgg taagcgtagc
    27001 gcatcaggca atgctgtgtt tgtcatcagt ctcagcgccc ggtgcaatac gtgacatcgt
    27061 gagccatgtc agtaagtggt taaattcgca gtggtagcat gccttccgtg atttcttact
    27121 aaccggagaa gtaatggaac tgcttttatt gagtaactcg acgctgccgg gtaaagcctg
    27181 gctggaacat gcactgccgc taattgctga acagttgcag ggtcgccgct cagcggtgtt
    27241 tatccctttc gctggcgtaa cgcagacctg tgatgattac acagcgaaaa cggctgcggt
    27301 tctcgctccg ctgggtgttt ctgtcaccgg tattcatagc gttgtcgatc ccgttgccgc
    27361 gattgaaaat gctgagatcg tgattgtcgg gggcgggaat actttccagt tgctgaaaca
    27421 gtgccgcgag cgcgggctgc tggcaccaat tactgacgtg gttaaacgtg gtgcgctgta
    27481 tattggctgg agcgcaggcg ctaaccttgc ttgcccaacc attcgtacca ccaacgatat
    27541 gccgattgtc gatccgcaag gtttcgatgc gctaaatctg ttcccgctgc aaatcaaccc
    27601 gcacttcacc aacgcgctgc cggaaggcca taaaggtgaa acccgtgagc agcgtattcg
    27661 cgaactgctg gtcgtcgcgc cagaactgac gattattggt ctaccggaag gtaactggat
    27721 cacggtgagt aaaggtcacg ctacgctggg tggcccgaac accacttatg tgtttaaggc
    27781 cggtgaagaa gcggtgccgc tggaagcggg tcaccgtttt taagtgaatt gccggatgca
    27841 tgatccggca tcttgttcat agtgcctgat gcgacgcttg acgcgtctta tcaggcctac
    27901 aggattcaca atattaatac tcgtcccgtc cttcatcttc atccggctgt tccagcacac
    27961 tgtaagccac cgaacaaaac agcgagttca gacgctgcat atctcccagt aagcctaaat
    28021 gtagcgaact ggtttcaatg ctctgcacgt tttgctgatg caggcgatcg acgtgggcgt
    28081 gggaatagcg gcgattaaga atgcgaaagc gatgcttgct gcggcgcaaa cgacgagcgc
    28141 tggtgacatc gccagagaag aacaccgaca ttgccagctt taaattactg agcaattgct
    28201 catagagcgc atccagttcc ttcaacccat caagcgaaaa tgcccgccgt gctgccagcg
    28261 atttatcagc aatttcgctg cccatgcgct cgacgatatc ggaagcctgt tcaaggttga
    28321 gcgacatttc aataatctcc gcccagcgac gcgactcttc ttctgccagt tcctcttttg
    28381 gcattcgcgc cagatacagc ttaatggcgg tgtagagaac gttgatatca tctgccagct
    28441 tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac tttattcagc ccttccatca
    28501 tctgttccat ggcgtcgcca atgcgcaggg tttcgcgcgc ggcgttcgcc agagcaagcg
    28561 tgggcgtatc cagcgcgctg acatccagat gtttaggccg tagctgggta tccagttccg
    28621 gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg gtcaacaaat ggcagcatga
    28681 ccaggcaacg tacaaggttg tagaagacgt ggaaatagat caccagttcc gcttttggca
    28741 atgacaactt ccccattgtc tctgccagca aatggacaaa cggcaggata atcaggctac
    28801 ccaccagctt aaacagcaaa ctacccagcg cgacacggcg ggctgcggca ttggcggcac
    28861 tgttgttgag catcgccagc aggccggaac cgaggttagc accaatcacc agacagagcg
    28921 ccacggggaa ggagataatg cctgcggcgg tcagagtcgc ggtcagcagt acagcagcaa
    28981 ggctggagta gctgataatg gcgaacatcg cgccaatcag cgcatccagc agaatatcgc
    29041 cggtcagcga ggcaaagatc acctgaacgc cgtttgcctg ggtgatcggc gttacggcct
    29101 gcacaatcaa ctccagcgcc agcaaaatca gcccaagacc aataccgacg cggcccagtt
    29161 gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac gccgataaaa ataagtaacg
    29221 gtgacagcca ggataagtcg aaggtgagaa tacgcgccat tagcgccgtc ccgacatctg
    29281 cacccagcac aatcaccaga gccggtgcga gggctaccag atcctgagcg acgaacgagg
    29341 tcaccagcat ggtggtggca ttactgctct gtaccagtgc ggtaacgccg atccccgcgc
    29401 aaaaggcgag cggctttttt tcgacgctcc ggctaaggac agtacgcaaa cgcgcgccga
    29461 agacgcgcat tacgccggtt cgaacaatat gagtccccca gaccagcagg gcgacggcag
    29521 aaagcaggtg aagcagcgtt aacacgagtt caggttctcc ttatcgttat atgttcctga
    29581 agcaattgaa tgaccaaagg accggaaaca cgctgactgg catctttact gtgccttttg
    29641 acattcgttt ttccggctaa gattattcca gcctgtttcc agtataagtg tttcatatta
    29701 ataaagagac agggcactca ttgagcgccc tgtttgttgt aaggaaaagt gacagatttg
    29761 tgaatcagtc cgcgtcatac cccagattcg gtgccagcca gcgctcaaca tcggagacgc
    29821 tcataccttt acggcgggca tagtcttcaa cctgatcgcg ctgaatttgc gccaccgcgt
    29881 agtacttgct gtccgggtgg ctgaaatacc aacccgaaac cgatgcacca ggccacatgg
    29941 cgaaagattc tgtgagtttc atgccagtat gtttttccac ttccagcagc tcccagatgg
    30001 tggctttttc cgtatgttcc gggcaggccg ggtagcccgg tgccggacgg atgccctggt
    30061 agttttcgcg gatcagttct tcgttgctga ggttctcgtt cggcgcatag ccccagtaga
    30121 ctttacgcac acgctcatgg agatactccg caaaggcttc ggctaaacgg tcggcaagcg
    30181 ctttcaccat gattttgttg taatcatcgt gctgcgcttc aaaggcatca gccagtgcgt
    30241 cctcttccag cccgccagtc acggcaaatg cgccgatgta atctgcttta ccagaaagct
    30301 tcggcgcaac gaagtcagcg agacagtagt tagcgaatcc cgttttttcg gtctgttgac
    30361 gcagatggtg gctgacgttg atcacatggg tgcgcgtttc gtcacggtag atttcaatgt
    30421 catcgcccac acggtttgcc gggaacaagc ccaccacgcc acgcggattc agcgttttct
    30481 cggcgcttaa tttatccagc atgtcgttgg cgtctttaaa cagccgctgc gcctcaacgc
    30541 ccaccacttc atcttccaga atgcgcggat acttcccggc cagcgaccag gtcataaaga
    30601 acggtgtcca gtcgatgtaa ttacgcagag tttcgatgct ggcttcaact tcctgcacgc
    30661 cgagacggtg cgccaccggc ggcgtgtaag cctgccagtc aaaagcgaag tcgttatcgc
    30721 gcgccgcttc cagcgtgacc ggtggtgtgc gcggtttctt gcgcccgtgc tgaatacgta
    30781 cggtttcata ctccttgcgg gtacgagcga caaaatcatc gcgctgagta tcggaaagca
    30841 gcgccgccac cacaccaacg gtgcgcgagg cattctgcac atacaccgtc gggccgctgt
    30901 agttctgctc aattttcacc gccgtgtgcg cttttgaggt cgtcgcgcca ccgatcagta
    30961 acggaatggt gaagccctga cgctccatct ctttcgccac gttaaccatc tcgtccagcg
    31021 acggcgtgat aagccccgaa agtccaatca gatcagcatt cacttcttta gcggtacgga
    31081 gaattttttc cgcaggcacc ataacgccga gatcgacaat ttcgtagttg ttacattgca
    31141 gcaccacacc aacgatattt ttaccgatgt cgtggacgtc gcccttcacg gtggcgatca
    31201 ccatcttgcc gttggttttg ccctgctctt tgctggcttc aataaacggt tcgaggtagg
    31261 ccactgcctg tttcatgacg cgcgccgatt tgaccacctg tggcaggaac attttcccct
    31321 cgccaaacag atcgccgacc acgttcatgc cgtccatcaa cgggccttca atcacttcaa
    31381 tcgggcgcgt agcctgctgg cgggcttctt cggtatcctg ctcgataaac tcagtaatgc
    31441 ctttgaccag cgagtattcc agacgtttat tcacttccca cgagcgccac tccgcctgct
    31501 gggcgttagc ggtgtcgtcg gttttgctgc cgcgatattt ctcggcaagc tccagtaaac
    31561 gttcagtgcc atcatcgcga cgattaagaa tcacatcttc caccgcgtcg cgcagttcag
    31621 cgggcaggtc gtcgtaaatc gccagttgcc cggcgttgac gatccccata tccatgccat
    31681 tgcgaatagc gtagtagagg aacaccgcgt gaatggcttc gcgcaccggt tcgttgccac
    31741 ggaatgagaa agaaacgtta gatacgccgc cggaaatcag cgcgtgcggc agttcgcgtt
    31801 tgatgtcttc gcaggcgccg ataaagtcct gcgcatagtt gttgtgctct tcaatgccgg
    31861 tggcgaccgc gaagatgttc gggtcgaaga tgatatcttc tggcgggaag ccaacctctt
    31921 cggtgaggat tttgtacgcc cgacggcaaa tctcgatttt ccgtgcgcgg gtatcggcct
    31981 gtccctgttc gtcaaaggcc attaccacca ccgctgcacc gtaacgacgc aacagtttcg
    32041 catgatggat aaaggcgtcg acgccctctt tcattgagat agagttaaca atgcctttgc
    32101 cctgaataca cttcagcccc ttttcgatga cctcccattt tgaggagtcg atcataatcg
    32161 gcacgcgagc gatatccggt tcaccggcaa tcagattgag aaaacgcacc atcgccgctt
    32221 cggcgtcgag catcccttca tccatattga tatcgataat ctgcgcgccg ttttccacct
    32281 gctggcgggc cacatccagt gcctcgctgt atttctcttc tttgatcagg cgcttgaact
    32341 tagcggaacc ggtgacgttg gtgcgttcac ccacgttcac aaacaggcta tcttcgccaa
    32401 tgttcagcgg ctccaggccg gacaaacggc aggctacggg gatttccggc agtttgcgcg
    32461 gcgctaatcc ttctactgca cgactcatcg ctgcaatatg ttgtggcgtg gtgccacagc
    32521 agccgccgac gatattgaga aagcccgctt ccgcccattc acgtatttgt tttgccatcg
    32581 tgtcggcgtc gagatcgtac tcaccaaagg cgttgggtag cccggcgttc gggtgcgcgg
    32641 tgacgtagca ttccgcaatc cgtgacagct cctgcacgta ctggcgcagt tcatcgggcc
    32701 ccagcgcaca gttcaggcca aaggtcagcg cttcggcgtg gcgcaatgag ttgtaaaatg
    32761 cttcggtggt ctgcccggag agcgtgcgcc cggaggcgtc ggtgatggtg ccagagatca
    32821 taatcggcag ctcaacgccc agcgtttcaa actccgtttt taccgcaaat accgccgctt
    32881 tggcgttaag ggtgtcgaaa acggtttcaa tcaggatcag atccgcacca ccttccacca
    32941 gcgctttggt ggactctcga taagccacca ccagcccgtc aaaagtgata ttacgaaatg
    33001 ccggatcgtt gacgtccgga gaaatagacg ccgtgcggtt ggttgggccg agaacaccgg
    33061 caacgtagcg cggtttctcc ggcgtgcgcg cggtccactc gtcagcacag gcgcgcgcca
    33121 gtttcgccgc cgcaaagttg atttccgccg acagggattc catctggtaa tccgccatcg
    33181 caatggtcgt ggagttgaag gtgttggttt cgatgatatc cgcgcccgct tcaaagtagg
    33241 cgttgtggat agcggcgatc acttccggtt tactgagtac cagcaggtcg ttgttgcctt
    33301 tgaggtcgca tggccagtcg gcaaagcgtt caccacgaaa atcggcttcg ttcagtcgat
    33361 agctctggat catggtgccc ataccgccgt ccagcaccag aatacgttca tttaactgcg
    33421 cacgcagttg ttccactttg ctgctcacac ttgctcccga cacgctcaac ccagatttaa
    33481 taaaaattca acaaaccata ctggcataaa cgcatctgtg gtaaaagcga ccaccacgca
    33541 acatgagatt tgttcaacat taactcatcg gatcagttca gcaactattg cattaactaa
    33601 caataaaaat gaaaatgatt tccacgatac agaaaaagga gactgtcatg gtcgcaccca
    33661 ttcccgcgaa acgcggcaga aaacccgccg ttgccaccgc accagcgact ggacaggttc
    33721 agtctttaac gcgtggcctg aaattactgg agtggattgc cgaatccaat ggcagtgtgg
    33781 cactcacgga actggcgcaa caagccgggt tacccaattc cacgacccac cgcctgctaa
    33841 ccacgatgca acagcagggt ttcgtgcgtc aggttggcga actgggacac tgggcaatcg
    33901 gcgcacatgc ctttatggtc ggcagcagct ttctccagag ccgtaatttg ttagcgattg
    33961 ttcaccctat cctgcgcaat ctaatggaag agtctggcga aacggtcaat atggcggtgc
    34021 ttgatcaaag cgatcacgaa gcgattatta tcgatcaggt acagtgtact cacctgatgc
    34081 gtatgtccgc acctatcggc ggtaaattgc cgatgcacgc ttccggtgca ggtaaagcct
    34141 ttttagccca actgagcgaa gaacaggtga cgaagctgct gcaccgcaaa gggttacatg
    34201 cctataccca cgcaacgctg gtgtctcctg tgcatttaaa agaagatctc gcccaaacgc
    34261 gcaaacgggg ctattcattt gacgatgagg aacatgcact ggggctacgt tgccttgcag
    34321 cgtgtatttt cgatgagcac cgtgaaccgt ttgccgcaat ttctatttcc ggaccgattt
    34381 cacgtattac cgatgaccgc gtgaccgagt ttggcgcgat ggtgattaaa gcggcgaagg
    34441 aagtgacgct ggcgtacggt ggaatgcgct gactttttct ggcgggcaga ggcaatattc
    34501 tgcccatcat acctgagtgg caatagaata agggtgtctg ttaatcgcat tgacgccaaa
    34561 ataacttaat gtcatacact tcacacactt ttattaactc agcattattt ttaaacatca
    34621 aaccacttaa ttattaacaa aatcgcaaga tctcattcca ccgaaacttt tacggctgca
    34681 ttatgagatt gagcgattta tgatatttct ttagttgtaa tcatattaaa ttttttcact
    34741 gttctgatag ttaaaattta agacatcaat aaacaatgag atatttaaat gattactcgt
    34801 attcctcgta gttctttctc tgcaaatatt aataatacag cccagacaaa tgaacaccaa
    34861 accctgagtg aattgtttta taaagaactc gaggataaat tttctggcaa ggagctggcg
    34921 acgcctctat taaaaagctt ctcagagaac tgtagacaaa atggtcgtca tatctttagc
    34981 aacaaggatt ttgtcattaa attttccacg tccgtcttac aagctgataa gaaagaaatt
    35041 acgataatta ataaaacgaa aacacgacac tcactcaaac cattgctcca atatttgaag
    35101 aatacctaat ggaaatttta cctcaacgct cagacactct tgataaacaa gaattaaacc
    35161 taaaatcaga tagaaaagaa aaagaattcc caagaattaa acttaatggt caatgttatt
    35221 ttccggggcg accccaaaac cgtatagtat gccgacacat tgctgcacaa tatattaatg
    35281 atatttatca gaatgttgat tacaaacccc atcaagatga ttattcttca gctgaaaaat
    35341 ttctcactca cttcaacaaa aaatgcaaaa accagacttt ggcgttgatt tccagccgtc
    35401 ctgaggggcg ttgcgttgct gcctgcggtg atttcgggct agttatgaaa gcatattttg
    35461 acaagatgga atcaaatggc atcagtgtta tggcagccat attactggtg gataaccatg
    35521 ctttgacggt ccggctaaga ataaagaaca caactgaagg atgtacccat tacgtggttt
    35581 cggtttatga tcctaatgta actaacgaca aaataagaat catgagcgaa agcaaagagg
    35641 atattaaaca ctattctctg atggatttta tgaatgtaga ttatagcctc ctgaaatggt
    35701 caaatgatca tgttattaac caatctgttg caataattcc agcacttccg aaagaacagc
    35761 tattgatatt aaaaggatc
//
LOCUS       DAJTVK010000031        34981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000031 DAJTVK010000000
VERSION     DAJTVK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.031"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..815)
                     /locus_tag="OO372_001570"
     CDS             complement(<1..815)
                     /locus_tag="OO372_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6341102.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO372_001571"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO372_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1397..1873)
                     /locus_tag="OO372_001572"
     CDS             complement(1397..1873)
                     /locus_tag="OO372_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6341103.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO372_001573"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="OO372_001573"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6341104.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="OO372_001574"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="OO372_001574"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6341105.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="OO372_001575"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="OO372_001575"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6341106.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="OO372_001576"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="OO372_001576"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6341107.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6911
                     /gene="bioD"
                     /locus_tag="OO372_001577"
     CDS             6234..6911
                     /gene="bioD"
                     /locus_tag="OO372_001577"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6341108.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7490..9511
                     /gene="uvrB"
                     /locus_tag="OO372_001578"
     CDS             7490..9511
                     /gene="uvrB"
                     /locus_tag="OO372_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6341109.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO372_001579"
     CDS             complement(9703..10611)
                     /gene="yvcK"
                     /locus_tag="OO372_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6341110.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10876..11020
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11008..11997
                     /gene="moaA"
                     /locus_tag="OO372_001580"
     CDS             11008..11997
                     /gene="moaA"
                     /locus_tag="OO372_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6341111.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12019..12531
                     /gene="moaB"
                     /locus_tag="OO372_001581"
     CDS             12019..12531
                     /gene="moaB"
                     /locus_tag="OO372_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6341112.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12534..13019
                     /gene="moaC"
                     /locus_tag="OO372_001582"
     CDS             12534..13019
                     /gene="moaC"
                     /locus_tag="OO372_001582"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6341113.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13012..13257
                     /gene="moaD"
                     /locus_tag="OO372_001583"
     CDS             13012..13257
                     /gene="moaD"
                     /locus_tag="OO372_001583"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6341114.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13259..13711
                     /gene="moaE"
                     /locus_tag="OO372_001584"
     CDS             13259..13711
                     /gene="moaE"
                     /locus_tag="OO372_001584"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6341115.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13848..14552
                     /locus_tag="OO372_001585"
     CDS             13848..14552
                     /locus_tag="OO372_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6341116.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14757..15470
                     /locus_tag="OO372_001586"
                     /pseudo
     CDS             14757..15470
                     /locus_tag="OO372_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(15535..16246)
                     /locus_tag="OO372_001587"
                     /pseudo
     CDS             complement(15535..16246)
                     /locus_tag="OO372_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO372_001588"
     CDS             complement(16246..17487)
                     /gene="clsB"
                     /locus_tag="OO372_001588"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6341117.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17484..18245)
                     /locus_tag="OO372_001589"
     CDS             complement(17484..18245)
                     /locus_tag="OO372_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6341118.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18378..18788
                     /locus_tag="OO372_001590"
     CDS             18378..18788
                     /locus_tag="OO372_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6341119.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18750..19856)
                     /locus_tag="OO372_001591"
     CDS             complement(18750..19856)
                     /locus_tag="OO372_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6341120.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19867..21000)
                     /locus_tag="OO372_001592"
     CDS             complement(19867..21000)
                     /locus_tag="OO372_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6341121.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="OO372_001593"
     CDS             complement(20993..22729)
                     /locus_tag="OO372_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6341122.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO372_001594"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="OO372_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6341123.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO372_001595"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="OO372_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6341124.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="OO372_001596"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="OO372_001596"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6341125.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO372_001597"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="OO372_001597"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6341126.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="OO372_001598"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="OO372_001598"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6341127.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="OO372_001599"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="OO372_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6341128.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="OO372_001600"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="OO372_001600"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6341129.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(31413..31673)
                     /locus_tag="OO372_001601"
     CDS             complement(31413..31673)
                     /locus_tag="OO372_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6341130.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO372_001602"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="OO372_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6341131.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO372_001603"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="OO372_001603"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6341132.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="OO372_001604"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="OO372_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8274 a         8975 c         9488 g         8244 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggatct gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg
     6901 ccttgttgta gccattctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac
     6961 tgcaatttaa ccaattaaat tctaaaataa tcacgaaaaa aattttactt ctgcctcatg
     7021 cggcgaatgt gggaattgcc caggcggcgg gggatagggg ctggagacag ttatccacta
     7081 ttcctgtgga taaccatgtg tattagagtt agaaaacacg aggcaagcga gagaatacgc
     7141 ggcttgcacg cgaattggcg ttaaagacgg ctcaaagaaa tatcttttat tttttaactg
     7201 gttagataaa tgcaatggca gtcactgaac aggcatctct tgccataaaa ctgtcatcac
     7261 tcatcttgac aaatgttaaa aaagccgttg ctttggggat aacccggtaa ggccggagtt
     7321 ttatctcgcc acagagtaaa ttttgctcat gattgacagc ggagtttacg ctgtatcaga
     7381 aatattatgg tgatgaactg tttttttatc cagtataatt tgttggcata attaagtacg
     7441 acgagtaaaa ttacatacct gcccgcccaa ctccttcagg tagcgactca tgagtaaacc
     7501 gttcaaactg aattccgctt ttaaaccttc tggcgatcag ccagaggcga ttcgacgtct
     7561 cgaagagggg ctggaagatg gcctggcgca ccagacgtta cttggcgtga ctggctcagg
     7621 gaaaaccttc accattgcca atgtcattgc tgaccttcag cgcccaacca tggtacttgc
     7681 gcccaacaaa acgctggcgg cccagctgta tggcgaaatg aaagagttct tcccggaaaa
     7741 cgcggtggag tatttcgtct cctactacga ctactatcag ccggaagcct atgtaccgag
     7801 ttccgacact ttcattgaga aagatgcctc ggttaacgaa catattgagc agatgcgttt
     7861 atccgccacc aaagcgatgc tggagcggcg tgatgtggtt gtagtggcct ctgtttccgc
     7921 gatttatggt ctgggcgatc ctgatttata tctcaagatg atgctccatc tcacggtcgg
     7981 tatgattatc gatcagcgcg cgattctgcg ccgactggcg gagctgcaat acgctcgtaa
     8041 tgatcaagca ttccagcgtg gtactttccg cgttcgtggc gaggtgatag atatcttccc
     8101 ggcagaatcg gatgacattg cacttcgcgt ggaactgttt gacgaggaag tggaacgatt
     8161 gtcgttattt gacccgctga ccgggcagat tgtttccact attccacgtt ttaccatcta
     8221 cccgaaaacg cactacgtca caccgcgcga gcgcatcgta caggcgatgg aggagatcaa
     8281 agaagaactg gccgccagac gcaaagtgct gttggaaaac aacaaactgc tggaagagca
     8341 gcggctgacc cagcgtaccc agtttgatct ggagatgatg aacgagctgg gctactgttc
     8401 ggggattgaa aactactcgc gcttcctctc cggtcgtgga ccgggtgagc caccgccgac
     8461 gctgtttgat tacctgcctg ccgatgggct gctggtggtc gatgaatctc acgtcaccat
     8521 tccacaaatt ggcggcatgt atcgcggtga ccgggcgcgt aaagagacac tggtggagta
     8581 cggcttccgc ctgccatcag cgctggataa ccgtccgctt aagtttgaag agttcgaagc
     8641 attagcgccg caaaccatct atgtttcggc gacgccgggt aattacgagc tggaaaaatc
     8701 cggcggcgat gtggtggatc aggtggtgcg tccaaccgga ttgcttgacc cgattatcga
     8761 agtgcggccg gtggcgacac aggttgatga tcttctttcg gagattcgtc agcgagcggc
     8821 aattaacgaa cgcgtactgg tcaccacact gaccaagcgg atggcggaag atcttaccga
     8881 atatctcgaa gaacatggcg agcgcgtgcg ttatcttcat tcagatatcg acaccgtcga
     8941 acgtatggag attatccgcg acttgcgtct gggtgagttc gacgtgctgg tagggatcaa
     9001 cttactgcgc gaaggtctgg atatgccgga agtgtcgctg gtggcgatcc tcgacgctga
     9061 caaagaaggc ttcctgcgtt ccgaacgttc gttgatccag actattggtc gtgcggcacg
     9121 taacgttaac ggtaaagcga ttctctacgg cgataagatc accccatcaa tggcgaaagc
     9181 gattggcgaa accgaacgtc gccgtgagaa acagcagaag tacaacgagg aacacggaat
     9241 tacgccgcaa ggcttgaaca agaaagtggt cgatatcctg gcgctggggc agaacattgc
     9301 caaaaccaaa gcgaagggca gaggaaaatc gcgcccgatt gttgagccgg ataatgtgcc
     9361 gatggatatg tcgcctaaag cgttgcaaca gaaaatccat gagctggaag ggttaatgat
     9421 gcaacacgcg cagaatctgg agttcgaaga agcggcacaa attcgtgacc agttgcatca
     9481 gctgcgtgag ctgtttatcg cggcttcgtg acaggataac gaagaagaca gatgacaaaa
     9541 gcaaaattgc ctgatgtgct acgcttatca ggcctacatg atctctgcaa tacattgaat
     9601 tagcgcggtt tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caacgcgcac
     9661 tttgtcttca ataggaagcc ggaatttttc ttcggatttc cgttaaccta aagcctgtaa
     9721 cgccttttcc agcgcgttat gtaacaactg gcggtcatga cgatacggaa tatcgctggc
     9781 ctccagtacc tcctgaatca caatccgctc tttcaccgcc gagacatcca cttttggccc
     9841 gacgatgacc gcatcaatga cctttttacc aacatactgc tccataattg ccagcttgct
     9901 ttccagcttc aaattagccg caggtaaact caactcacgc cccagattgc cgatatagac
     9961 catcggcgct ggcgtgcggc gtaatgcctg ggcgatttcc ttcagcagca gaattggcat
    10021 caggctggta taaaaactgc caggcccaat aatgatgaga tccgcttcat tgatagcgtg
    10081 aaccgcctca cgcgttgcgg gtacattcgg cgttaataat aactcttgaa tcggcgtagt
    10141 taactggtcg atattgacct cgccgtaaac ttcatgcccc tgatcgtcaa tcgccatcag
    10201 atcgacggga tgctctgaca ttggaatcaa atgcgtatcc actttcagca gattacgaat
    10261 taaattgatg gcttccagag gccgcacgct aaggtgatcc agcgccttta acatcaagtt
    10321 tccgagatta tgaccggaaa gttcgccatt gccgccaaaa cggtattcaa acatcgcgga
    10381 ggcgacgctc ggttccgtta tcagttggtt gaggcagttg cgcatatcgc cccaggcaat
    10441 gccgccttct gaacggcgaa tacgccccgt cgagccacca ttatccgtgg tggtgacgat
    10501 acccgttaaa cgagaaccca aagacgaaag tgatgagaga acgcgtccca gtccatgccc
    10561 tccgccgaga gcaacgacac gatcaagatc agccagcgta cgattgcgca tatagtttcc
    10621 tggagtcaga ttatccgcgc tacagtagcg caaattgcgg ggaaacagca attaacctgc
    10681 caatattgag gatacaaact attttcttct acctctaaag gacgatgcac gctatgcctc
    10741 cctgatgatg tatatcaaag taaaactgcc attttccctt attctgtagc gaaatagcac
    10801 gatcatgacg ctatatacat gattacatag cgaaagtgtg gatggtaaaa atctcattta
    10861 cacgctagta tcggcttaac cactaaacac tctagcctct gcacctgggt caactgatac
    10921 ggtgctttgg ccgtgacaat gctcgtaaag attgccacca gggcgaagga agaaatgact
    10981 tcgcctcccg tatctggaaa ggtgtacatg gcttcacaac tgactgatgc atttgcgcgt
    11041 aagttttact acttgcgcct gtcgattacc gatgtgtgta actttcgttg cacctactgc
    11101 ctgccggatg gctacaaacc gagcggcgtc accaataaag gctttcttac cgtcgatgaa
    11161 attcgccggg ttacgcgcgc cttcgccagt ctgggcaccg aaaaagtgcg cctgacagga
    11221 ggagagccgt ctttacgccg cgactttacc gatatcatcg ccgctgtgcg ggaaaacgac
    11281 gctatccgcc agattgcggt caccaccaat ggttaccgtc tggaacgcga tgtggcgaac
    11341 tggcgcgatg cgggacttac tggcattaac gtcagtgtcg acagtctgga cgcccgccag
    11401 tttcacgcta ttaccgggca ggataaattc aaccaggtca tggcagggat cgatgctgca
    11461 tttgaggtcg gttttgagaa ggtcaaagtc aataccgtgc tgatgcgtga tgttaatcat
    11521 caccagctcg acacctttct gaactggatc cagcatcgcc ctatccagct gcgtttcatc
    11581 gaactgatgg aaacgggcga gggcagtgag ctcttccgta agcatcacat ctctggtcag
    11641 gttctgcgtg acgagctact gcgtcgcggc tggatccacc aattacgtca acgcagcgac
    11701 ggtcctgcgc aagtcttttg ccatccagat tacgccggag agattggcct tatcatgccg
    11761 tatgaaaaag acttctgcgc cacttgcaac cgcctgcgcg tttcctccat tggtaaactc
    11821 catctctgcc tgtttggtga aggcggcgtt aacctgcgcg atctgctgga agacgatacc
    11881 cagcaacagg cgctggaagc gcgtatttca gcggcgctgc gggagaagaa acagacccat
    11941 ttcctgcatc aaaacaacac cggtattacg caaaacttat cgtacattgg cggctaaaac
    12001 gtcaaaagga gagatcagat gagtcaggta agcactgaat ttatcccgac ccgtattgct
    12061 attcttacgg tttctaatcg tcgcggtgaa gaagacgata cctccggtca ctatttgcgc
    12121 gattcggcgc aagaagcggg ccatcacgtt gtcgataaag ccattgtgaa agaaaaccgc
    12181 tacgctattc gcgctcaggt atctgcgtgg atcgccagcg acgatgtaca agtggtgttg
    12241 attacggggg ggactggcct gacggaaggt gatcaggctc ccgaagcatt gctgccgttg
    12301 ttcgaccgtg aagttgaagg ttttggtgaa gtgttccgga tgttgtcgtt tgaagagatt
    12361 ggcacttcca cgttgcaatc tcgtgcggta gcgggcgtcg ccaacaaaac gctgattttc
    12421 gccatgccgg gttcgaccaa agcgtgccgt accgcatggg aaaatatcat cgcgccgcag
    12481 ctggatgccc gtacgcgtcc gtgtaatttc catccacatt tgaagaaata agtatgtcgc
    12541 aactgaccca tatcaacgcc gctggcgaag cgcacatggt ggatgtctcc gccaaagcgg
    12601 aaaccgtgcg tgaagcgcgg gcggaagcct ttgtcaccat gcgcagcgag acgctggcga
    12661 tgattattga tggtcgccac cacaaaggcg acgtatttgc cactgcgcgt attgtcggta
    12721 ttcaggcggc aaaacgcacc tgggatctga tcccgctgtg tcacccgctg atgctcagca
    12781 aagttgaagt caatttacag gccgagccgg agcacaatcg ggtgcgtata gaaaccttat
    12841 gccgcctgac cgggaaaacc ggtgtcgaaa tggaagcgtt aaccgcggcc tccgtggcgg
    12901 cgctgaccat ttatgacatg tgcaaagcgg tgcaaaaaga tatggtgatt ggtccggtac
    12961 gtttgctggc gaagagcggc ggcaagtcgg gtgactttaa ggtggaagcg gatgattaaa
    13021 gttctttttt tcgcccaggt gcgcgagttg gtgggaacag atgcaaccga agtggctgcg
    13081 gattacccaa ccgttgaagc gttacgccag cacctggctg cgcagagcga tcgctgggcg
    13141 ctggcgctgg aagatggcaa attgctggct gccgtcaacc agacgctggt gagttttgac
    13201 catccgctga ctgacggcga cgaagtagct ttcttcccgc cggtaaccgg aggttaagat
    13261 ggcagaaacc aaaattgttg tcggcccgca gccgttcagc gtaggagaag agtacccgtg
    13321 gctggctgag cgtgacgaag acggtgcagt ggtcacgttt accggtaagg tgcgcaacca
    13381 taacctgggt gacagcgtca aagcattaac cctcgaacac tatccgggga tgactgaaaa
    13441 agcactggca gaaatcgttg atgaagcgcg taaccgctgg ccgctggggc gcgtcacggt
    13501 gattcaccgc atcggggaat tatggccggg cgatgaaatc gtttttgtcg gtgtcaccag
    13561 tgcgcatcgc agcagtgcgt ttgaagccgg gcagtttatt atggattatc tcaaaacccg
    13621 cgcaccgttc tggaagcgcg aagccacgcc ggaaggcgac cgctgggttg aagcccggga
    13681 gagcgatcag caggcggcaa aacgctggta gttttttgtt agccggataa ggcaccgctg
    13741 cgtccggcaa tttcaccgct cagtacatgc gtttacgctg cgtcgatgca cagcctcatc
    13801 attttgcagt atccttaaga tattccttat atcttcagga gatcgtcatg gacagattcc
    13861 cacgttctga ttcaatcgta caaccccggg ctggcttgca aacttatatg gcgcaagtct
    13921 atggctggat gaccgttggc ttgttgctga ccgcatttgt tgcctggtat gcggctaatt
    13981 ccgcggccgt gatggagctg ttgttcacta accgtgtctt tttaatcggt ctgatcatcg
    14041 cgcaattagc attggttatt gtgttatcag cgatgattca aaagctgagc gcaggtgtaa
    14101 cgacgatgct ctttatgctt tattcggcgc tgacgggtct tacgctttcc agtatattca
    14161 ttgtctatac cgctgcttct atcgccagta ctttcgtcgt tactgccggg atgttcggcg
    14221 caatgagcct gtacggttac accacgaagc gcgatttaag tggctttggc aatatgctgt
    14281 ttatggcgtt aatcggcatt gtgctggcat cgctggtcaa cttctggctg aaaagcgaag
    14341 cattgatgtg ggcagttacc tacatcggcg tgattgtctt tgtcggattg acggcgtatg
    14401 acactcagaa attgaaaaat atgggtgagc agattgatac ccgcgacacg tcgaacctgc
    14461 gcaaatattc cattcttggc gcgttaacct tgtatctgga cttcatcaac ctgttcctga
    14521 tgttgttacg gatcttcggc aaccgccgtt aattcttttt caccatcact atcccggcca
    14581 tgtgtcggga tttttttctt actaaaaacg acacttaaca agtaccaggt ataaaatatg
    14641 ccttatgagc gagcggaatc gttttccgta tccataaata caaaagtatt ttgtatgcgt
    14701 ggttttaaat ttattgatgg tgaatattaa tattttttct aaggacaact aaaagaatgg
    14761 aatcatactc gcaaaacagc aataaattag attttcagca cgaggccagg atataaaacg
    14821 gtatatggct cattaccgct ttaggtttgg tggcaaccgc aggactagcc tggggagcta
    14881 agtatctcga aattacggca accaaatatg attcaccacc aatgtatgtc gctatagggt
    14941 tattattact ttgtatgtat ggcttaagta aggatatcaa caagataaat gccgccatcg
    15001 cgggcgtaat atatctgttt ttactctctt tggtggcgat tgtcgttgca agtttagttc
    15061 ctgtatatgc cattatcatc gtgttcagca ctgcgggcgc gatgttttta atcagtatgc
    15121 tggccggttt attatttaat gttgagcctg gttctcaccg ttttatcatt atgatgacgt
    15181 tgacagggtt ggccctggta atcatcgtga atgcggcatt aatgagtgaa cggcccattt
    15241 gggtaataag ttgcttaatg attgtgttat ggccaggcat tatctcgcat ggacgaaata
    15301 agctccttga actggcagga aaatgccata gtgaagagtt gtggagtccg gttcgttgcg
    15361 cttttacagg tgcattaaca ctctattact attttatcgg cttctttggg atacttgccg
    15421 cgatagctat aacgcttgtc tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc
    15481 acatactaaa gaacttaact aaacttcaca tcgccgcttc atttttcgcc cgtagcccag
    15541 tacaaagaaa tagttcacgc tttgaccaag taacagccag ataatcgccc ccattaccat
    15601 ccagttaacg ctggaaatca gcatctgagc gagggcgaat ttccatgaag gcagcaccag
    15661 cttttgccct ttgatggtca tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac
    15721 cgcgataatc atcagtaagc caatgccgag aatacgcagc gtagtttgat cgacatacca
    15781 gtgatccggc aactccacca cgcctgcggt aaagataatc cctgccagta aaatatagcc
    15841 cagccagttg gtggtaatac tgagcgagaa aatccgcgta atagtgctac cgggcaaccc
    15901 cagacgagag tacaaacgat agcgcatacc aatgccgccg acccaggtac tgagcgtcag
    15961 gttgaaggcg tagcagataa acgacaccag catcacctgg cgcttcgcca gtttgtgacc
    16021 gcagtaaaag cgggcgagca ggtcatagca gccgtaaatc agatagctga cgaccaccag
    16081 cgaccgcact aagcagcgca acgcgattgt agtcgcggat gaccttccag acctcttccc
    16141 agtccacttt tttggcgtag accaccagca acacaatcac cgcgataaaa aacagccagg
    16201 tgaggatctt ctttgctaag cgccagcgcg ggtgtgattt actcatcagg gttttacccc
    16261 cgtgttttca gtttctaccc gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac
    16321 ctgcgccaga cgtggcgtgt gtgccggaag ccagccaacc agcgccggga agtggcgtaa
    16381 aaagtggaac gccagcacgc ttttggtcag gttccaccag gtgcgtttgg gcagcatggt
    16441 ttcatccacc tgctgacaat ctgcggcgat aatgccgttc agattatcgc gcagcgtctg
    16501 gttaaaatta cgatcgtgga tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg
    16561 atcgagatta ctggacccta ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg
    16621 gagcgggcgg cggcggtact caaacacctg aacgccgcct ttaaccagat agttatacag
    16681 caagcgcgcg ccgactctga caatcggcat atccggttcg ccctgaatga tcagtttgat
    16741 ccgcaccccg cggcgtgccg ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa
    16801 gtaggcgttg gcgataatga cttcccgccg cgcctgagtg agcattttca gatagtggcg
    16861 ttctatatca tcgcggtgtt cttcgttatc gcgccagacc agcaatacct gcgcgtctcc
    16921 cggctggcgg ttctcttccg ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg
    16981 tccaggcagg ttttccagct caaactggag aatatcttcg acaatcggcc cttcaaggcg
    17041 taccgcgtaa tcctgtttag cctctggacc gtagctggac atatgctcgg cggagtaatt
    17101 cagcccgcca ataaaggcga tacgcgcgtc gatcaccaca attttgcgat gcatccggcg
    17161 aaacacattg gtgcgcatac caaaaaggcg agggcgggga tcgtagtagc ggaacactac
    17221 gccagctgcc gtcagttcat tgacaaactc atcgctgaga tccggcgaac cgtagccatc
    17281 cagcaagact tccgctttaa ccccgcgttg cgctgctgcc agtagtgccg catgcagctg
    17341 tttgccgacg ttatcctcaa accagataaa cgtttcaaga atgatgcgtt cttgtgcctc
    17401 gccaatcgcc ttaaacaccg cgggataata ttgctcgccg ttttccagca actggatctt
    17461 attgccttcg cgccagctac atttcataaa tgaatctccg cacttaaagg ggcatgatca
    17521 gaaaggtgtc gccatgtccg cagcggcaac gcggttggcg cgctggcgct ggcatttttg
    17581 acgtagatcc tgtccagtcg tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt
    17641 ccgtgggcgc gggtaaaaat ctcatccagt ccggcctgca cttttaacgg atgattagct
    17701 ttttgccgcc agtcattgaa atcacccgcc accaataccg gttcgccgtc cggtagctca
    17761 ttcacccatt cggcgagcat cgcaagctgc gcctgacggt gcgcctcacg caggcccaga
    17821 tgtacgcaca tcacatgaat cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc
    17881 acgccgcgct tttccgcacc atcgaccgaa acatcgcgat tctcataatg ttcaatggga
    17941 taacgcgaca gtacggcgtt gccgtgatgc ccttccgggt atacggcatt gcgaccgtag
    18001 gcaaaatcgc tccacatagt gtcggcgaga aactcgtagt gcgaggtatc gggccagttt
    18061 tccacatgca gcggatgaac ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg
    18121 gcgctgacgg tacgcacggc atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg
    18181 gtaaagcctt tgtgaatgtt gatggtgagc accttgaacg aaaattgttg tgtttgatcg
    18241 ggcataattt tcctgtcttt gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa
    18301 aaggtgcggt cttacggaat tttccgtaaa gttcggtact ctgagtaagt agagataaat
    18361 tcttcaggag agaagccatg aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt
    18421 gctggcagat tatgttgcat ttactggtag tggcgctgct ggtggtgggc tggatgagta
    18481 agactctggt tcacgtcggc gtgggattat gcgcactgta ttgtgtcacg gtagtgatga
    18541 tgctggtgtt tcagcgccac cccgagcaac gctggcgtga ggtggcagac gtgctggaag
    18601 agctgaccac gacctggtat tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg
    18661 tactggaaaa caactttttg ctggcaattg cagggctggc gatccttgct ggcccggcgg
    18721 tagtgtcttt gctggcgaaa gataagaagt tacatcacct tacgtctaaa catcgcgtac
    18781 gccgctgacc ctgtcgtggc cgttatcacc agtagcggcc acaaactatt ccacacaata
    18841 tccagactcg catccttcaa ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg
    18901 ttaatccacg tcaggttttg cagccatact ggcatgtttt ccaccggaga aacgtaaccg
    18961 gaaaggagaa tggcgggcat cataaaaaca aacacgccga taaacgcctg ctgttgtgtc
    19021 gagcagagtg atgaaatcaa cagaccgaat cccaccagcg ataaaccgta aatcaccatc
    19081 gtaaagtaga acagcgccag cgatccggcg aaggggattt gatacgccca gataccaatc
    19141 gccagcacaa tggtggcctg gaaggtggcg acaattaacg ccggtacggc tttgccgatg
    19201 aagatctgcc aggtggtgag cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt
    19261 tcgcgggcga cggaaagtga agtgacgatc attacgccga tagtggtgat catggcgatc
    19321 agcgacggca ccacaaacca tttgtagtca agattcgggt tataccagtt gcgtaccacc
    19381 agctcgctgt tgttaggttt cggttttcct tccagcagct cctgctgata atttttgacg
    19441 atctgttgca ggtagttggc ggcaatttgc gcactgttgg agttacgccc gtcgaggatc
    19501 aactgcaacg gcgcggtctg gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc
    19561 accagtagta acgccttttg tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc
    19621 agcagcacat gggtaaaggc gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc
    19681 tcgccgttat cttcatcgta gatggcgatg gtggcgttag tcacttccag cgtggcggcg
    19741 aacgggaaca ggatcacctg aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt
    19801 tcgcgcagca acgactgcaa ctctttgcgg attaacgtcc ataagcgatg aaacatgctc
    19861 ttctccctaa tccagccgac gtttggtttt cagccacgtc aggccgataa acatcaccgc
    19921 cgaagcgatc aaaaacagca cgtttaccac cagcaccact ggaatattcc cggcgaggaa
    19981 caggctttgc agggtgctga cgaaataacg agcgggaata atgtacgtca ccgcgcggat
    20041 caccgcgggc atactgtcga tctgaaaaat aaagccggaa agcataatcg acggcaggaa
    20101 cgcggcgttc agggcgacct gagcggcatt gaactggttg cgggtaatcg tggaaatcag
    20161 cagccccatc cccagggtac tgagtaaaaa caggctggag ataaaaaaca gaataagcag
    20221 cgaaccgcga tacggcacgc cgagaataaa cactgacacc agcatacaca gcagcatcgc
    20281 cagcatcccg agaaagtaat aagggatcag cttacacagc agcagttccg tgcgggtaat
    20341 ctccgtagag agcagagcct ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga
    20401 ggtgagaatc gcgccgatga ccgtcatgat gatggtcact gcaccgggga taatgaagtg
    20461 ctggctaatc gccgccgggt taaaccagta gcgggtttgt acatcaataa gcggttcaaa
    20521 agtctgcccg ttgtcctccg ctcgctgcat ttgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgtg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 caggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaaggccg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgt tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg catcttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAJTVK010000032        33999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000032 DAJTVK010000000
VERSION     DAJTVK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.032"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..514
                     /locus_tag="OO372_001605"
                     /pseudo
     CDS             12..514
                     /locus_tag="OO372_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO372_001606"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="OO372_001606"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6341133.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO372_001607"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="OO372_001607"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6341134.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO372_001608"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="OO372_001608"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6341135.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO372_001609"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="OO372_001609"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6341136.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO372_001610"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="OO372_001610"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6341137.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO372_001611"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="OO372_001611"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6341138.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO372_001612"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="OO372_001612"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6341139.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO372_001613"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="OO372_001613"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6341140.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO372_001614"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="OO372_001614"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6341141.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO372_001615"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="OO372_001615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6341142.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO372_001616"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="OO372_001616"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6341143.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO372_001617"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="OO372_001617"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6341144.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO372_001618"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="OO372_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6341145.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO372_001619"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="OO372_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6341146.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="OO372_001620"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="OO372_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6341147.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="OO372_001621"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="OO372_001621"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6341148.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="OO372_001622"
     CDS             complement(20046..21878)
                     /locus_tag="OO372_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6341149.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO372_001623"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="OO372_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="OO372_001624"
     CDS             complement(22624..23118)
                     /locus_tag="OO372_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6341150.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /locus_tag="OO372_001625"
     CDS             complement(23201..23656)
                     /locus_tag="OO372_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6341151.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="OO372_001626"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="OO372_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6341152.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="OO372_001627"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="OO372_001627"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6341153.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="OO372_001628"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="OO372_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6341154.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO372_001629"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="OO372_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6341155.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO372_001630"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="OO372_001630"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6341156.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO372_001631"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="OO372_001631"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6341157.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="OO372_001632"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="OO372_001632"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6341158.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="OO372_001633"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="OO372_001633"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6341159.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="OO372_001634"
     CDS             32232..33125
                     /locus_tag="OO372_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6341160.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO372_001635"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="OO372_001635"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6341161.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8480 a         9277 c         8564 g         7678 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agtacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatagccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAJTVK010000033        33995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000033 DAJTVK010000000
VERSION     DAJTVK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.033"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OO372_001636"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="OO372_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OO372_001637"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="OO372_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR6341162.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4169..4831
                     /gene="acrS"
                     /locus_tag="OO372_001638"
     CDS             4169..4831
                     /gene="acrS"
                     /locus_tag="OO372_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR6341163.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4834..5013)
                     /locus_tag="OO372_001639"
     CDS             complement(4834..5013)
                     /locus_tag="OO372_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR6341164.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OO372_001640"
     CDS             complement(5097..5981)
                     /gene="yhdJ"
                     /locus_tag="OO372_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6341165.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OO372_001641"
     CDS             complement(6067..6363)
                     /gene="fis"
                     /locus_tag="OO372_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6341166.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OO372_001642"
     CDS             complement(6389..7354)
                     /gene="dusB"
                     /locus_tag="OO372_001642"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6341167.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OO372_001643"
     CDS             complement(7683..8564)
                     /gene="prmA"
                     /locus_tag="OO372_001643"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6341168.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OO372_001644"
     CDS             complement(8576..10027)
                     /gene="panF"
                     /locus_tag="OO372_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6341169.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10017..10259)
                     /locus_tag="OO372_001645"
     CDS             complement(10017..10259)
                     /locus_tag="OO372_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6341170.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OO372_001646"
     CDS             complement(10368..11717)
                     /gene="accC"
                     /locus_tag="OO372_001646"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6341171.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OO372_001647"
                     /gene_synonym="fabE"
     CDS             complement(11728..12198)
                     /gene="accB"
                     /locus_tag="OO372_001647"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6341172.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13176..14150)
                     /locus_tag="OO372_001648"
     CDS             complement(13176..14150)
                     /locus_tag="OO372_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6341173.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14302..16242
                     /gene="csrD"
                     /locus_tag="OO372_001649"
     CDS             14302..16242
                     /gene="csrD"
                     /locus_tag="OO372_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6341174.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16547..17590
                     /gene="mreB"
                     /locus_tag="OO372_001650"
     CDS             16547..17590
                     /gene="mreB"
                     /locus_tag="OO372_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6341175.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17656..18759
                     /gene="mreC"
                     /locus_tag="OO372_001651"
     CDS             17656..18759
                     /gene="mreC"
                     /locus_tag="OO372_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6341176.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18759..19247
                     /gene="mreD"
                     /locus_tag="OO372_001652"
     CDS             18759..19247
                     /gene="mreD"
                     /locus_tag="OO372_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6341177.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19256..19849
                     /gene="yhdE"
                     /locus_tag="OO372_001653"
     CDS             19256..19849
                     /gene="yhdE"
                     /locus_tag="OO372_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6341178.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19839..21308
                     /gene="rng"
                     /locus_tag="OO372_001654"
     CDS             19839..21308
                     /gene="rng"
                     /locus_tag="OO372_001654"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6341179.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21376..25176
                     /gene="yhdP"
                     /locus_tag="OO372_001655"
     CDS             21376..25176
                     /gene="yhdP"
                     /locus_tag="OO372_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6341180.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25332..26777
                     /gene="tldD"
                     /locus_tag="OO372_001656"
     CDS             25332..26777
                     /gene="tldD"
                     /locus_tag="OO372_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6341181.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26905..27770)
                     /locus_tag="OO372_001657"
                     /pseudo
     CDS             complement(26905..27770)
                     /locus_tag="OO372_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27953..28156
                     /locus_tag="OO372_001658"
     CDS             27953..28156
                     /locus_tag="OO372_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6341182.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28164..29096
                     /gene="aaeA"
                     /locus_tag="OO372_001659"
     CDS             28164..29096
                     /gene="aaeA"
                     /locus_tag="OO372_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6341183.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29102..31069
                     /gene="aaeB"
                     /locus_tag="OO372_001660"
     CDS             29102..31069
                     /gene="aaeB"
                     /locus_tag="OO372_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6341184.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31161..31433
                     /locus_tag="OO372_001661"
     CDS             31161..31433
                     /locus_tag="OO372_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6341185.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OO372_001662"
     CDS             complement(31489..31752)
                     /gene="yhcN"
                     /locus_tag="OO372_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6341186.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OO372_001663"
     CDS             complement(32117..32587)
                     /gene="argR"
                     /locus_tag="OO372_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6341187.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33022..33960
                     /gene="mdh"
                     /locus_tag="OO372_001664"
     CDS             33022..33960
                     /gene="mdh"
                     /locus_tag="OO372_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6341188.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8254 a         8453 c         8900 g         8388 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt ctactgtttt attttgacgc
     3961 gggttgaaag aggcagaatt aaaacctcgt aaattgaaat atatattcat gtggcgaatg
     4021 tattttaggt aaataatata tattatttat ttgacgattt atagcaactc aatgttaccc
     4081 tctcgtataa atacacatta gatgatagat taaccttcgc tattttctca ccctgtgtcg
     4141 aatatattta tttcctgaat aattaatcat ggcaaaaaga accaaagccg aagctctgaa
     4201 gacccggcaa gaactgattg aaactgccat cgcccagttt gcgcaacatg gcgtaagcaa
     4261 gacgacgttc aacgacattg ccgatgccgc taacgttacg cgtggcgcta tctactggca
     4321 cttcgagaac aagacccaac tgtttaatga gatgtggttg caacagcctt cattgcggga
     4381 gttaatccag gaacacttga cggctggatt agagcatgac ccgtttcaac aattgcgtga
     4441 aaaattgatt gtcggcttgc aatatattgc caaaattccc cgccagcagg cgttgctgaa
     4501 aatcttatat cacaaatgtg aatttaatga tgggatgctg gccgagggag tgatacgcga
     4561 aaagatgggc tttaatccgc agactctccg cgaagtattg caggcgtgtc agcaacaagg
     4621 ttgtgtagca aataacctcg atttagatgt tgtgatgatt attattgatg gtgccttcag
     4681 cggaattgtt caaaactggt taatgaatat ggcgggttat gatctttata aacaagcacc
     4741 cgcgctggtc gataacgtat taagaatgtt catgccagat gaaaacataa cgaaattaat
     4801 tcatcaaaca aatgaattaa gtgtcatgta aattcacatt ctgtcctgaa aattcagttc
     4861 gtcacagtta accagtttta gtggataaac tgagttcata ttacggcatt tgtctgtttc
     4921 tgttgtcagc agttcaatcc actgcatcag taaagtatca aacagaaaaa cgaagacagc
     4981 gaaaacgacg agccaccaat acttgcgaat cattacctga gcctgtgcat gtttaaccaa
     5041 gtctggcagg aaatatacac gaaatgaata atctgaaaac caggcttaca cttcctttac
     5101 tttgcaatga gatcggggtc aacttcgctt aaccggcttc gttttgacag gttgccggtc
     5161 tttctttttt tcactttcgc cagttcttcc gcggagtaat gcgacgcgac atccagccgt
     5221 cgaagcccca ttttgatgta ctcgctgttg atctcaatac caatgaattt tcgtccgctg
     5281 gcgatggcta cggcaccggt agtaaagcta ccggcaaacg ggtcgagaac gatatcgcct
     5341 gggttggaag aggcgagaat aatgcgtttc agtaaggctt ccggtttttg cgtcgggtgg
     5401 ttctcatatt catccatcaa ataacgcacg cgcggaaaat cccagacgtt acccggtact
     5461 ttttgatgat tgtatggctg tggaggattt ttgcgataat cgatcaacgc gcgctgcgat
     5521 ccggttttgg cttctaccag aatagcatca ccgttgaatg tgtagttctt tgcgtctttc
     5581 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
     5641 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
     5701 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
     5761 cggtggcact ctacaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
     5821 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gatcagatcg
     5881 acgctttcag taggtagctt tttaagttcg gcaagggcat caccgtgaat aatggtctta
     5941 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
     6001 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
     6061 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
     6121 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
     6181 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
     6241 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
     6301 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
     6361 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
     6421 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
     6481 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
     6541 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     6601 aaggcagcgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     6661 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     6721 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     6781 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     6841 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     6901 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     6961 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     7021 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     7081 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga tcgctaccag
     7141 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     7201 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     7261 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     7321 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     7381 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     7441 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgagataa ttactcataa
     7501 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     7561 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
     7621 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     7681 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
     7741 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     7801 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
     7861 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     7921 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
     7981 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     8041 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
     8101 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
     8161 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     8221 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     8281 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     8341 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     8401 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     8461 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
     8521 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     8581 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     8641 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     8701 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     8761 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     8821 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     8881 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     8941 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     9001 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     9061 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     9121 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     9181 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     9241 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     9301 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     9361 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     9421 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     9481 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     9541 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     9601 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     9661 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     9721 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     9781 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     9841 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     9901 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     9961 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
    10021 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
    10081 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
    10141 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
    10201 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
    10261 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
    10321 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
    10381 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
    10441 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
    10501 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
    10561 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
    10621 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
    10681 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
    10741 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
    10801 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
    10861 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
    10921 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
    10981 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
    11041 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
    11101 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
    11161 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
    11221 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
    11281 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
    11341 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
    11401 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
    11461 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
    11521 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
    11581 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
    11641 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
    11701 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
    11761 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
    11821 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
    11881 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
    11941 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
    12001 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
    12061 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
    12121 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
    12181 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
    12241 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
    12301 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
    12361 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
    12421 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
    12481 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
    12541 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
    12601 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
    12661 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
    12721 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
    12781 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
    12841 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
    12901 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
    12961 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
    13021 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
    13081 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
    13141 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
    13201 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
    13261 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
    13321 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
    13381 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
    13441 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
    13501 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
    13561 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
    13621 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
    13681 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
    13741 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
    13801 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
    13861 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
    13921 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
    13981 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
    14041 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
    14101 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
    14161 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
    14221 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
    14281 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
    14341 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
    14401 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
    14461 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
    14521 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
    14581 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
    14641 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
    14701 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
    14761 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
    14821 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
    14881 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
    14941 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
    15001 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
    15061 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgatacttgg
    15121 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
    15181 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgctgtta
    15241 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
    15301 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
    15361 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
    15421 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
    15481 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
    15541 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    15601 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    15661 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    15721 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
    15781 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    15841 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    15901 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    15961 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    16021 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    16081 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    16141 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    16201 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    16261 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    16321 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    16381 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    16441 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    16501 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    16561 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    16621 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    16681 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    16741 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    16801 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    16861 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    16921 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    16981 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    17041 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    17101 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    17161 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    17221 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    17281 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    17341 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    17401 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    17461 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
    17521 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    17581 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    17641 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    17701 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    17761 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    17821 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    17881 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    17941 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    18001 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    18061 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    18121 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    18181 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    18241 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    18301 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    18361 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    18421 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    18481 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    18541 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    18601 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    18661 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    18721 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    18781 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    18841 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    18901 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    18961 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    19021 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    19081 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    19141 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    19201 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    19261 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    19321 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    19381 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    19441 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    19501 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    19561 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    19621 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    19681 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    19741 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    19801 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    19861 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    19921 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    19981 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    20041 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    20101 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    20161 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    20221 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    20281 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    20341 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    20401 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    20461 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    20521 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    20581 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    20641 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    20701 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    20761 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    20821 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    20881 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    20941 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    21001 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    21061 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    21121 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    21181 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    21241 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    21301 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    21361 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    21421 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    21481 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    21541 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    21601 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    21661 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    21721 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    21781 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    21841 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    21901 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    21961 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    22021 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    22081 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    22141 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    22201 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    22261 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    22321 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    22381 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    22441 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    22501 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    22561 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    22621 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    22681 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    22741 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    22801 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    22861 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    22921 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    22981 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    23041 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    23101 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    23161 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    23221 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    23281 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    23341 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    23401 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    23461 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    23521 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    23581 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    23641 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    23701 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    23761 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    23821 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    23881 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    23941 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    24001 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    24061 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    24121 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    24181 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    24241 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    24301 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    24361 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    24421 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    24481 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    24541 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    24601 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    24661 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    24721 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    24781 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    24841 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    24901 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    24961 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    25021 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    25081 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    25141 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    25201 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    25261 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    25321 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    25381 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    25441 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    25501 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    25561 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    25621 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    25681 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    25741 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    25801 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    25861 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    25921 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    25981 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    26041 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    26101 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    26161 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    26221 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    26281 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    26341 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    26401 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    26461 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    26521 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    26581 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    26641 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    26701 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    26761 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    26821 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    26881 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    26941 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    27001 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    27061 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    27121 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    27181 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    27241 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    27301 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    27361 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    27421 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    27481 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    27541 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    27601 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    27661 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    27721 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    27781 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    27841 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    27901 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    27961 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    28021 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    28081 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    28141 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    28201 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    28261 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    28321 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    28381 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    28441 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    28501 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    28561 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    28621 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    28681 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    28741 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    28801 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    28861 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    28921 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    28981 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    29041 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    29101 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    29161 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    29221 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    29281 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    29341 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    29401 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    29461 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    29521 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    29581 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    29641 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    29701 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    29761 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    29821 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    29881 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    29941 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    30001 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    30061 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    30121 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    30181 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    30241 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    30301 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    30361 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    30421 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    30481 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    30541 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    30601 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    30661 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    30721 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    30781 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    30841 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    30901 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    30961 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    31021 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    31081 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    31141 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    31201 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    31261 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    31321 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    31381 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    31441 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    31501 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    31561 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    31621 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    31681 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    31741 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    31801 catatcacaa aatcaagcat acactaatta ttgaactaat taataccaca ctaactgttt
    31861 cagttgatac taagtgttaa aaatgtgaat tttaataaca aaaaagaatt aaaaacacgg
    31921 ttatttattg ttaattctag tattttcaat tagataatga attttacata atgtgctttt
    31981 tttaccgtat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact attcgttaaa
    32041 ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc cgccgccaga
    32101 agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct tcgtacaggt
    32161 ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc
    32221 ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc
    32281 cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc accagattct
    32341 tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa accatttcca
    32401 ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag actttagact
    32461 gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc tgggagctaa
    32521 atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc ttagccgagc
    32581 ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac agatgaattt
    32641 ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag cgggatttta
    32701 tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg tttaaaagta
    32761 aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat tgtcaccacc
    32821 tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag gttcctgatt
    32881 acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga gatgtggtca
    32941 ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat cttagtttat
    33001 caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc
    33061 caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact ctctctgtat
    33121 gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc tactgctgtg
    33181 aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt
    33241 cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt
    33301 aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc gaaagcgtgc
    33361 attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga agtgctgaaa
    33421 aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga catcattcgt
    33481 tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga agtgccggtt
    33541 attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc tggcgttagt
    33601 tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg tactgaagtg
    33661 gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc agctgcacgt
    33721 tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac
    33781 gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac
    33841 ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa
    33901 ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt taataagtaa
    33961 ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAJTVK010000034        33316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000034 DAJTVK010000000
VERSION     DAJTVK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.034"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..665
                     /locus_tag="OO372_001665"
     CDS             <1..665
                     /locus_tag="OO372_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCR6341189.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            735..1511
                     /gene="yaaA"
                     /locus_tag="OO372_001666"
     CDS             735..1511
                     /gene="yaaA"
                     /locus_tag="OO372_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6341190.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1664..1960)
                     /locus_tag="OO372_001667"
     CDS             complement(1664..1960)
                     /locus_tag="OO372_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6341191.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2174..3460)
                     /gene="thrC"
                     /locus_tag="OO372_001668"
     CDS             complement(2174..3460)
                     /gene="thrC"
                     /locus_tag="OO372_001668"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6341192.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3461..4393)
                     /gene="thrB"
                     /locus_tag="OO372_001669"
     CDS             complement(3461..4393)
                     /gene="thrB"
                     /locus_tag="OO372_001669"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6341193.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4395..6857)
                     /gene="thrA"
                     /locus_tag="OO372_001670"
     CDS             complement(4395..6857)
                     /gene="thrA"
                     /locus_tag="OO372_001670"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6341194.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6884..7003)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6939..7004)
                     /gene="thrL"
                     /locus_tag="OO372_001671"
     CDS             complement(6939..7004)
                     /gene="thrL"
                     /locus_tag="OO372_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6341195.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7218..7904)
                     /locus_tag="OO372_001672"
     CDS             complement(7218..7904)
                     /locus_tag="OO372_001672"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6341196.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8304..8444)
                     /locus_tag="OO372_001673"
     CDS             complement(8304..8444)
                     /locus_tag="OO372_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341197.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8540..9256
                     /gene="arcA"
                     /locus_tag="OO372_001674"
     CDS             8540..9256
                     /gene="arcA"
                     /locus_tag="OO372_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6341198.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9316..10668)
                     /gene="creD"
                     /locus_tag="OO372_001675"
     CDS             complement(9316..10668)
                     /gene="creD"
                     /locus_tag="OO372_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6341199.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10726..12150)
                     /gene="creC"
                     /locus_tag="OO372_001676"
     CDS             complement(10726..12150)
                     /gene="creC"
                     /locus_tag="OO372_001676"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6341200.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12150..12839)
                     /gene="creB"
                     /locus_tag="OO372_001677"
     CDS             complement(12150..12839)
                     /gene="creB"
                     /locus_tag="OO372_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6341201.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12852..13325)
                     /gene="creA"
                     /locus_tag="OO372_001678"
     CDS             complement(12852..13325)
                     /gene="creA"
                     /locus_tag="OO372_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6341202.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13536..14405
                     /gene="robA"
                     /locus_tag="OO372_001679"
     CDS             13536..14405
                     /gene="robA"
                     /locus_tag="OO372_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6341203.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14402..15049)
                     /gene="gpmB"
                     /locus_tag="OO372_001680"
     CDS             complement(14402..15049)
                     /gene="gpmB"
                     /locus_tag="OO372_001680"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6341204.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15101..15622
                     /gene="yjjX"
                     /locus_tag="OO372_001681"
     CDS             15101..15622
                     /gene="yjjX"
                     /locus_tag="OO372_001681"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR6341205.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15707..16033)
                     /gene="trpR"
                     /locus_tag="OO372_001682"
     CDS             complement(15707..16033)
                     /gene="trpR"
                     /locus_tag="OO372_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6341206.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16123..18060)
                     /gene="sltY"
                     /locus_tag="OO372_001683"
     CDS             complement(16123..18060)
                     /gene="sltY"
                     /locus_tag="OO372_001683"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6341207.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18271..19938
                     /gene="ettA"
                     /locus_tag="OO372_001684"
     CDS             18271..19938
                     /gene="ettA"
                     /locus_tag="OO372_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6341208.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20245..21477)
                     /gene="nadR"
                     /locus_tag="OO372_001685"
     CDS             complement(20245..21477)
                     /gene="nadR"
                     /locus_tag="OO372_001685"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6341209.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21498..22880)
                     /gene="radA"
                     /locus_tag="OO372_001686"
     CDS             complement(21498..22880)
                     /gene="radA"
                     /locus_tag="OO372_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6341210.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22929..23897)
                     /gene="serB"
                     /locus_tag="OO372_001687"
     CDS             complement(22929..23897)
                     /gene="serB"
                     /locus_tag="OO372_001687"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6341211.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24003..24647
                     /locus_tag="OO372_001688"
     CDS             24003..24647
                     /locus_tag="OO372_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6341212.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24675..25691
                     /gene="lplA"
                     /locus_tag="OO372_001689"
     CDS             24675..25691
                     /gene="lplA"
                     /locus_tag="OO372_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR6341213.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25723..25872
                     /locus_tag="OO372_001690"
     CDS             25723..25872
                     /locus_tag="OO372_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341214.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(26147..26866)
                     /gene="deoD"
                     /locus_tag="OO372_001691"
     CDS             complement(26147..26866)
                     /gene="deoD"
                     /locus_tag="OO372_001691"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6341215.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26946..28169)
                     /gene="deoB"
                     /locus_tag="OO372_001692"
     CDS             complement(26946..28169)
                     /gene="deoB"
                     /locus_tag="OO372_001692"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6341216.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28221..29543)
                     /gene="deoA"
                     /locus_tag="OO372_001693"
     CDS             complement(28221..29543)
                     /gene="deoA"
                     /locus_tag="OO372_001693"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6341217.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(29670..30449)
                     /gene="deoC"
                     /locus_tag="OO372_001694"
     CDS             complement(29670..30449)
                     /gene="deoC"
                     /locus_tag="OO372_001694"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6341218.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30707..32257
                     /locus_tag="OO372_001695"
     CDS             30707..32257
                     /locus_tag="OO372_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6341219.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            32229..33092
                     /locus_tag="OO372_001696"
     CDS             32229..33092
                     /locus_tag="OO372_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6341220.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         7917 a         9145 c         8397 g         7857 t
ORIGIN      
        1 taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag gcgggaatgg
       61 gttcgacgcc aaacgcggca gcggcagcgg cgtcctggcc tccgcatccg gcagcgcaag
      121 ggattgtcca gatgattggc atttttatcg acaccctggt catctgtacg gcaagcgcca
      181 tgctgatatt actggcgggt aacgacacaa cctacatgcc gctggaaggt attcagctta
      241 tccagaaggc gatgcgggtg ctaatgggtt cctggggtgc tgagtttgtt accctcgtgg
      301 ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa aacaatctct
      361 tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc accttcgcaa
      421 cggtcatcgg cggcaccttg ctaagtcttc cgctgatgtg gcaactggca gatatcataa
      481 tggcctgcat ggctattacc aatttgaccg ccattttact gctctcgcct gtggttcata
      541 ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg tttgatccgt
      601 tgcgttatcc ggatatcggt cgccagcttt ctccggacgc gtgggatgat gtttcgcagg
      661 agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc ataaatttcc
      721 tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat taccaaagcc
      781 cgttgaccac cacgcgctat acgctgccgg agctgttaga caattcccag cacttgatcc
      841 atgaagcgcg gaaactgacg cctccgcaga ttagcacgct gatgcgcatc agcgacaaac
      901 tggcgggtat caacgccgct cgctttcatg actggcagcc agatttcacg ccggagaatg
      961 cccgccaggc tattttggcg tttaaaggtg atgtctacac cggcttgcag gccgaaactt
     1021 tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc ggcttgtatg
     1081 gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg atccgtcttg
     1141 agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc aacaagctga
     1201 acgaggcgct cgcagcacaa ggcgataatg tggtgattaa tctggcgtca gatgagtatt
     1261 ttaaatcggt gaagccgaag aaattgaatg ccgagattat caagccggtg ttcctcgatg
     1321 agaagaacgg caaatttaag ataatcagct tctacgctaa gaaagcacgc ggtctgatga
     1381 gtcgtttcat tattgaaaat cggctgacca aaccagagca actgactggt tttaatagcg
     1441 aaggttactt ctttgatgaa gcttcctcca gcaatggcga actggtgttt aaacgctacg
     1501 agcagcgtta agtttgcagt caatgccgga tgcggcgtga acgccttatc cggcctacaa
     1561 aagcatgcag attcaatata ttgcagaatt cacgtaggcc tgataagacg cgccagcgtc
     1621 gcatcaggcg ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc
     1681 ctggaccatg accgccgtga tgatcatgag gagctttctt atggtggcgc ggcggtggcg
     1741 gcggtccgtg tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt
     1801 ggtcgcgcca gtgacctcca tcccagtaat agccacgatt atcacgatcg cctatctgta
     1861 attttactga cgggactaac gtaatttccg cagcctgtgc tgccatggga gcgaccagaa
     1921 ccagggaaag tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt
     1981 gcccgttatg ggtcgatatc cggagattac gcgatgagaa cgacggttgt tattctccgc
     2041 aatcctaatc tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgttttt
     2101 ctccataatt tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga
     2161 taatgaatag attttactga tgattcatca tcaatttacg caacgcagca aaatcggcgg
     2221 gcagattatg tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca
     2281 acgtttcacc gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga
     2341 ggaacaagcc atattcgcct ggattcaact ggtcacacag cgcacgatac gctacggcag
     2401 cgtgcggctc cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt
     2461 catcatccac ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact
     2521 cttccacacg cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct
     2581 gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt
     2641 tggtcgcagc aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg
     2701 tcaaatcgcc gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcttcctgcg
     2761 gcagctgcgc aactgcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa
     2821 tcgagttagc cgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct
     2881 tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc
     2941 ccaacgtaca gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa
     3001 ccactttcac attcggcaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg
     3061 aggtcgcggt cagaatggtc actggcttat cgcccgcaat atgggtcagc atttgtgcca
     3121 taaagcgacc gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac
     3181 cgacatcgct ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct
     3241 cttccaggat ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc
     3301 gggtgacaaa atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt
     3361 catgcggaaa aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc
     3421 tgacctgctc gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg
     3481 cgcccgccgt atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct
     3541 tacccaacca gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg
     3601 tcgggccgga gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg
     3661 cctgccggaa gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca
     3721 gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc
     3781 gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg taaaatagcc cgggcttctg
     3841 ccgtcgagac tttaattccc ggatacgcca gcacccacag ccactcatca aaccctggca
     3901 cttgctggct gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg
     3961 gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc ttccagctcg cccatcaaag
     4021 ccagcaaacg ggtgtcatta agtggcttgc cgcagtgttc attcatcgcc atcagcgccg
     4081 cgaccaccga acaggcgctg gagcctaagc ccgagccgat cggcatattc ttttccagag
     4141 tcatcgccac tggaatttgc ttgcccagct ccaggcaaaa acgctcccag cactgataaa
     4201 cgatattttc ccgtggttct gacggcagct tatcggcaaa gcgtccgagg ttgttgagac
     4261 tgaatgtctc tgccgcctca accgtgacta catctccgag caatgcacca tcaacgggtg
     4321 tcaccgccgc cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat
     4381 aaactttaac catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag
     4441 acaccagcag cggtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc
     4501 tgataatagt ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga
     4561 tcattcccat ccacttcggc aatcttcacg cggcagacac catcttcatc aatattgcca
     4621 acatagcgca aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg
     4681 tcgagctgtg acagattcgc cataaaagcg gcaacatcac cctcggcgtt aaactctgcg
     4741 ggcagcacag gttcaatttc aatatccgcc agttccagtt cacgtcccgt ttcacgagcg
     4801 agaatcaata gtttacgcgc cacatccata ccagaaagat catctcgcgg gtccggttcg
     4861 gtataaccca tttcccgcgc cagcgtggtc gcctcggaga aactcatgcc ttcgtctaac
     4921 ttgccgaaga tataagaaag cgaacctgaa agaatgccgg agaacttcac caattcatca
     4981 cctgcattga gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg
     5041 tcatagagga atttacgccg cgatttttcc gccgcataac gcaactgatg atagtaatcc
     5101 atcgacgagg tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaggaag
     5161 tcggcatatt gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc
     5221 agatgatatt ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgt
     5281 gcaagttctt cctgccagtt ttccagatta aggccatgca cactagtgag cagagccttc
     5341 gagttggcaa cgccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgctga
     5401 cgcttcagtt gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg
     5461 ataacctgat cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca
     5521 tcgttattta ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg
     5581 ttgatattgg cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc
     5641 ataccatcac ctaccaccga gataatggcc agccgttcca tcaccgccag cggctccagt
     5701 aagccttctt tcagttccag gtagaactct tcctgcattg cccgttcagc ccgcacacag
     5761 tcgctttgcg gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc
     5821 acggaaatac gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc gaccattcct
     5881 ttcatccccg ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg
     5941 accggtaatt cgccttcatc acgactggca ccaatgagcg tacctggtgc ttgaggattt
     6001 ccggtatttt taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg
     6061 tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc
     6121 aacaacctcg catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc
     6181 caaatctcgc aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa
     6241 ccgttgcgtc caagtaccac cagttcgcct ttttcattac cggcggtgaa gcctgccatc
     6301 agcaccatgt gatcagccgg aatacgactt gccgcaatac ggcgggtgga ctcagcaata
     6361 tcgacggtag attcgaggta atgccccact gccagtagtt tttcgaccgg atcgataacg
     6421 gtaacgttgt gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg
     6481 ccacggcaaa tcagcgcagc gttaatgcta tccgggcact gccctaacaa actaatgcca
     6541 tgcaggacat gttttatttg ggcaaattcc tggtcgacga aagttttcaa ttgcgccagc
     6601 gggaatcccg gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca
     6661 tcgctgatat tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg
     6721 tggttggtga ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg
     6781 ctttccagaa tatcggcaac ccgcagaaaa cgttctgcat ttgccactga tgtaccgccg
     6841 aacttcaaca ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa aagcccgcac
     6901 tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc gttacctgtg
     6961 gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt tgtgtactct
     7021 gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag tgacctaagt
     7081 caataaaatt ttaatttact catggcaggt aaccagttca gaagctgcta tcagacactc
     7141 tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa gctgaaaaat
     7201 acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag caaacggtgc
     7261 aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca gtcgaccagt
     7321 tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc gcgtaaagct
     7381 tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat taatgttgct
     7441 aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata atccgccacc
     7501 atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcag cccggaatct
     7561 tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc taacagcggc
     7621 accagttcta ctggcgtggc gtagtaatga tatttcgccc gactgcgcgc agtggtggcg
     7681 acagtgaaat cgacatcgtg taacgattca gccagtgttg ggaaaacttt aatattatca
     7741 ataacatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg tgcctgacta
     7801 tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc tgccccaata
     7861 ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc actcttcttg
     7921 accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg aatttacaaa
     7981 ttcttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaggct aaactatttc
     8041 ctgactgtac taacggttta attgttaaaa aatgctacat atccttctgt ttacttagga
     8101 taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag ttgtatgatg
     8161 caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac gatgatttca
     8221 tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt agttttattg
     8281 ggtgtccggc ccctcttagc ctgttatgtt gctgttaaaa tggttaggat gacagccgtt
     8341 tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat gttgttgacg
     8401 ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg ccgatcatgt
     8461 taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt cgatttagtt
     8521 ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag acgagttggt
     8581 aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt tcgaagcgac
     8641 agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg tgatcatgga
     8701 tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg agcaggcgaa
     8761 tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc tcggcctcga
     8821 aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga cgattcgtgc
     8881 acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac gtcgtagcgt
     8941 tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt tgatcggccc
     9001 tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc acttctgtga
     9061 aaacccaggc aaaattcagt cccgtgctga actgctgaag aaaatgaccg gccgtgagct
     9121 gaaaccacac gaccgtactg tagacgtgac gatccgccgt attcgtaaac atttcgaatc
     9181 tacgccggat acgccggaaa tcatcgccac catccacggt gaaggttatc gcttctgtgg
     9241 tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa acggcgcttt ttagcgccgt
     9301 ttttattttt caaccttatt tccagatacg taattcatcg tccgttgtaa cttctttact
     9361 ggctttcatt ttcggcagtg aaaacgcata ccagtcgata ttacgagtca caaacatcat
     9421 gccggccagc gccaccacca gcacactggt tcccaacaac agcgcgctat cgacagagtt
     9481 gagcagtccc cacatcactc catccagcaa caacagcgcg agggtaaaca acatgctatt
     9541 gcgccagcct ttcaataccg cctgcaaata aataccgttc attaacgccc caatcagact
     9601 ggcgattatc catgccacgg taaaaccgat atgttcagaa agcgccagca agagcaaata
     9661 aaacatcacc aatgaaagcc ccaccagcaa atattgcatt gggtgtaaac gttgcgcggt
     9721 gagcgtttca aaaacaaaga acgccataaa agtcagtgca atcagcagaa tggcgtactt
     9781 agtcgcccgg tccgttaatt ggtattgatc ggctggcgtc gtcactgcga cgctaaacgc
     9841 gaggaagttt tcccagccgg tatcattgcc tgaagcaaaa cgctcaccga gattattagc
     9901 aaaccagctg ctttgccatt gcgcctgaaa acctgactcg ctaacttccc gtttggctgg
     9961 caaaaaatca cctaaaaaac tgggatgcgg ccagttgctg gttaaggtca tttcgctatt
    10021 acgcccggca ggcaccacag aaagatcgcc ggtaccgctt aaattcaggg ccatattcag
    10081 cttcaggttc tgcttccgcc agtccccttc gggtaaaggg atatgcacgc cctgcccgcc
    10141 ttgctctaac ccggtgccgg gttcaatggt cagcgccgtt ccgttaactt caggcgcttt
    10201 caccacacca ataccacgcg catccccgac gctaatcaca ataaatggct tgccaaaggt
    10261 gatatttggc gcgtcgagtt cgctaagacg cgaaacatcg aaatcggctt ttagcgttaa
    10321 atcactgtgc cagacctgac cggtataaat ccctatcttg cgttcttcca cgttctgatt
    10381 gccatcaacc atcaatgact caggtaacca aaaatggata aaacttcgtt tccgctccac
    10441 ggttttatcc tcttcctgta ccgtataaag ctcggtcaca ggaatagcga taagcggccc
    10501 aacaagtttt tgcggcccgc tggtactttg gcgaatcgca tcttccacat cgctacggta
    10561 atcggcacgt tcgacaatca cctgccgaat cagcattatc ggaatcaaca acagcaatac
    10621 tgcaccaaac agggtggtca ttttccagaa cagggaggat ttcaacatag tcttctcctt
    10681 tgcaatggca gcaggatacg aaggctatgt gggaagaatt tgaagctatg tgaagtgacg
    10741 gtgaagtcga agcgaggcca gcacgccacc ttcctgcacg ttgcgcagcg tgacttcgcc
    10801 gttaaacaaa cgggcgacct cactgacgaa cgccaacccc agaccgctgc ttttttgccc
    10861 atttgcacgc ggcaaagagt aaaagcgttc aaaaatacgt gacagcgcgt agtcaggaat
    10921 accactaccg gtatccagca ccttaagcgt gacgtgttcc tgatccactt cggcgcttag
    10981 cgttatacga ccgctctcgg gggtaaaatc gatggcgtta tccagtaaat tccccagcgc
    11041 ctgatccagt aacgccggtt cagcagcaac attaacctcg gtgggcgtaa catgtagagt
    11101 gatgtttttt tctgccaact gcacggtgcg cgcttcgctg acgcggcgga ataatgccgc
    11161 cacatcaaca gcagtcaaaa cgacttcctg acgattctcc agtcttgcct ggcgtagtaa
    11221 cgtttccacc agtgcctgca ttcgcgcatt ttgcgtcaga atgttgtcag taaaacgagc
    11281 cactacttca ggcggcggac cttcgcgtaa aatttccgcc gcgccacgaa tcgccgccag
    11341 tgggcttttt agctcatggg ttaacgcata aacatactgc tcaatatagt ttttcccttc
    11401 cagcttcacg cgcatacttt ccagcgcctg cgcgagttta cgcagctcgc tactaccgag
    11461 atcgggaaga ggaacgggct tattgtcagt gacggaatca gcatagcgag tgagccgggc
    11521 aatggagcgg ttgatccacc aaaccatgcc tgcgccaatc accagtgcga tccccaacaa
    11581 aatggcgctg gcccataaaa ttcgccgctc gctgcgttta atgaccggag ccatcgccgc
    11641 gttcggtttg cctacgctca aaacgccaat aagccgcgag ccgtccataa tcggcgcggc
    11701 aacatacatc accgaacttt cgggatcggc aggattttgc aacgtgctac gcgcaccata
    11761 ctgaccacgc aacgttagcc agacgtcatt ccagcgcgaa taatcctgcc caacggcttt
    11821 atttgccgaa tcgaacaata ctttgccctg cgcatcggtc atatagacat gatattcatt
    11881 gcgcactttg ttaatgccac cgatattggc gcgaaacggg cgatgttgta gctgattaaa
    11941 cgcctgagcc agttgcccat gcgttgggtc cccagagagc aaatcgggac gcgccagctc
    12001 cgccagcaac gttgcggtat cgattaacgt cccctccgtt gctcttcgca cgcccggttt
    12061 aacttctttg acaaaaatag ccagtacgaa ccaggccgcc accgccacca gtaaaaaata
    12121 gcccagcagc aaccgcatgc cgatacgcat tacaggcccc tcaggctata tcccatgccg
    12181 cgatgagtat taatcggaga aagatcgggg ttgatggcgc gcagcttggc acgcaacgtt
    12241 ttaatgtggg tatcgacggt gcgatcgtag gtgtcctgcg catcttccca cacgctatcc
    12301 attagttgct ggcgggacca gacgcggcct ggcgacttga gtaacgtctt cagcaataaa
    12361 aactcatacc gcgtcagcgt taatggcgtg tcaaaccagc tgatctgcgc cgcgggttca
    12421 ttcaattcaa aatgaccaat acggatgacg ggagacggcg acgagaactt cttcacccga
    12481 cgcagtaagg tgcgcaccct ggcgcacact tcgcggggtg aaaacggttt agccacatag
    12541 tcgtcagcgc caatttccag cccaagcagg cgatcgacct cttcacttcg ggccgtcagg
    12601 aacagtacag gtaacgccgg atggagcgcc agtaactggc ggcacaattc aaagccgctg
    12661 atatcaggca aaccaacatc gagaatcatg acgtcgggaa cctgctggcg agctttatcc
    12721 agcaccggca agcctcgctc aaagacctcg acggcaaaac cttcctgctg caacatgtag
    12781 accagcgtgt cggctatccc ttgctcatct tccactaacc agaccgtttc ccgttgcata
    12841 aatcgcctct gttattgccg ccacggcatg acaggaaccg cgctaatcgc gtttttcggc
    12901 gaaccttcta caactttgtc ggagtaagcc agataagcga gcgcgttgcg tttggcgtca
    12961 taaaagcgca cgacctgtaa cgacttaaag accagggacg tgcgtttttt gaatactacc
    13021 tcgccctgag ctttgccgtt tttaatacga tccgacagtt caatcggccc gacttgctga
    13081 caagaaatag ccgcatcgga ggtatcttcc gccagaccca atcccccttt aataccacct
    13141 gttttcgccc ggctcacata acaggtgaca tttttcacat cgggatcgtc aaaggcttcc
    13201 acaacaattt tgtgatccgg gccgatcatt ttaaatacgg tgtcgaccga accaatctct
    13261 tctgcatgag ccaatggccc cagcattatc aggcttaaag acaggatcaa atgcttgtat
    13321 ttcatattgt taccattttt atctattaca cgagcaatta gttcgtcacg gtaaataaca
    13381 tattgattaa gaacaaaatc tcaatacttt tatttccgtc aagccctaaa acatactcta
    13441 ctaagaaaaa aacactgaat gctaaaacag caaaaaatgc tattatccaa ttacctgatg
    13501 tcaggtgctc gttgttgaaa ggatgaggat attttatgga tcaggccggc attattcgcg
    13561 accttttaat ctggctggaa ggtcatctgg atcagcccct gtcgctcgac aatgtagcgg
    13621 cgaaagcagg ttattccaag tggcacttac agagaatgtt taaagatgtc actggccatg
    13681 ctattggcgc gtatattcgt gctcgtcgtt tgtcgaaatc ggcggtcgca ctacgcctga
    13741 ctgcgcgtcc gattctggac atcgcgctgc aataccgctt cgactctcaa cagacattta
    13801 cccgcgcatt caagaagcag tttgcccaga ctcctgcact ttaccgccgt tctcctgaat
    13861 ggagcgcctt tggtattcgc ccgccgctac gtctgggtga attcacaatg ccagagcaca
    13921 aatttgtcac cctggaagat acgccgctga ttggtgttac ccagagctac tcctgttcgc
    13981 tggagcaaat ctctgatttc cgccatgaaa tgcgttatca gttctggcac gattttctcg
    14041 gcaacgcgcc gaccattccg ccagtgctct acggcctgaa tgaaacgcgt ccgagtcagg
    14101 ataaagacga cgaacaagag gtattctata ccaccgcgtt agcccaggat caggcagatg
    14161 gctatgtact gacggggcat ccggtgatgc tgcagggcgg cgaatatgtg atgtttacct
    14221 atgaaggtct gggaaccggc gtgcaggagt ttatcctgac ggtatacgga acgtgcatgc
    14281 caatgctcaa cctgacgcgc cgtaaaggtc aggatattga gcgatactac ccggcagaag
    14341 acgccaaagc gggagatcgc ccaattaatc tgcgctgtga actgctgatt cctatccgcc
    14401 gttaacgctg cagctcatct aatgcagggg cgtccagatg cgagatgtcc cctgcggttt
    14461 caacgaccca gccagacgcc agccacaggc tttcctgata atccacgcgc gaaatagaac
    14521 agttacgcag acgtaagcgg cgctctgccc atgctggtaa tccgagaatc gtactcacca
    14581 gacatcccag tgcaatacca tggctcacca gcagcggtcg gcttccctgc ggtaagtccc
    14641 ggcaagattc cagtgccgca ttgacgcgat cgctgagctc ctgcattgac tcgccttcag
    14701 gaatacgccc gtcaacggtg ccattgacca gctgccgacg ccagttctct tcttcttcgg
    14761 tcagagaatc gatatgtctt ttctccagca cacccatgtt taattcacgc aggcgagaat
    14821 caaagatgat gtcacagccg caggcctggg cgatgatttc cgccgtacgc cgggtgcgtc
    14881 ctaaatcgct actgataata tgcgtaatgc caagctcttt ggcacgggtt gccacctgca
    14941 tcgcctgttg ctcacctttg gcggtcagcg ggctgtcaga ctggccctga atacgtcgct
    15001 cggcgttcca ctgcgtttca ccgtggcgga ctaggtatac ctgtaacatg ctttttttcc
    15061 gtatactgct gtaacgttaa ttttctgagt aatgctgatt atgcaccaag ttatctgtgc
    15121 gaccaccaat cccgctaaaa ttcaggccat tctgcaggca tttcacgaga tcttcggcga
    15181 aggatcctgc catattgcat ccgttgccgt cgagagcggt gtaccggaac agccctttgg
    15241 cagtgaggaa acgcgcgctg gcgcacgaaa tcgggtagca aatgcccgcc gtttacttcc
    15301 agaggctgat ttttgggtgg cgattgaagc tggcatcgat ggtgacagca ctttcagttg
    15361 ggtggtgatt gaaaacacca gccagcgcgg cgaggcgcgt tcggctactt tgccgttacc
    15421 tgcggtgatt ctgcagaaag tgcgtgaagg tgaggcgctt ggtcccgtga tgtcgcgtta
    15481 taccggtatt gatgagattg gtcgtaagga aggggcgatc ggcgtgttta ccgccgggaa
    15541 actcactcgc gccagcgtct atcaccaggc ggtgatcctg gcgctgagtc cgtttcacaa
    15601 tgccgtgtat caggcattgt aggacggata aggcgttcac gccgcatccg gcattcggtg
    15661 cacgatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaatcaa tcgcttttca
    15721 gcaacacctc ttccagccac tggcgcagct ccacgggcgc ggctttcagg ctgttagatc
    15781 cacgcgtaat cgtcgcgatg cccgcgccga gttcattttt taactcacgc tgactcattt
    15841 cgccacgcaa cagctcttcg acaatacgca cgcgagtccc caacgcttcg cgctcatctg
    15901 gcgtcagcat caggtttaac aacggtaaat ggagatcgtt ttggtaggca ttcttaagca
    15961 ggtcgacaaa acgtaaccac tcctggtgac gctgttctgc catcgctgct gaatagggtg
    16021 attgttgggc cataatatgt cgccattgtt agcgggggaa gcaaaatgcc tcccccggtt
    16081 gtactcgcta aagagtacga tagcatatca taaacgtgcg gatcagtaac gacgtcccca
    16141 ttccgtggcg ctcatcaacg tcggtttatc ccccatgaaa taacggtagt aagcgtcata
    16201 agccagcacg ttcttcacat aaccgcgcgt ctcggagaac ggaatactct cgacaaatgc
    16261 cacagcgtcg atacgcccgg cgctgttgcc aagccaggtt cgcacccgtc caggtccggc
    16321 gttataagct gctgaggaga aaatacggtt attgccaaac tgctgataaa catattgcag
    16381 gtaactggtg ccaatgttga tattcgtttc cggatccagc aattgcccag ggctgctata
    16441 accaggaata gagaacatct tcaccgtatg ggtcgctgta ccaggcataa tctgcatcag
    16501 gccgctggcc cctaccggtg atttcacttt cggattccag gcgctctcct gacgggcaat
    16561 cgccatcgca tagctttgcg ggatctcttt cccactggtg taacgtttga aaagatcgtt
    16621 gtaagccagc gggaatcgct cttccagatg atcccaaagc ttcccggcga tcgttgcctg
    16681 aacgctaaga tcccaccatt ggttattgaa agcataccga gccagttgag cctgctctgt
    16741 ttttgacttg ctcttcacca gattggccca ctcgctacgc gcggtgttat cgagattcca
    16801 gtacatcaac tcgcgcacgc gcgccatctc cggcccctga gtcagggcgc tgtcaacatt
    16861 ctgcggcgct ttatcaatct tcagctcata ctcttctccg atgcgttgtg ctgcaaccat
    16921 cgggtagaaa ccacgctgtt gcatgagttg atgcaaaatc tctttcgctt cagcttcacg
    16981 tccgcgttcc agcaataaat ccacctgcca gtaacgccat tcatctttct cttttgcttc
    17041 catcggcaga cgcgccagcc aggtattcag gccgcgacga tcgccggtgc caagtgccat
    17101 tcgcacacgg cgttcaatca acgacgtgga ttgcgagcgc ataatgacgt catcgcgcca
    17161 tttcgcctgc tcgtcggtga catcgttgcc catcaaacgc caggcgacga tatcgcgcag
    17221 ctcctgaatc tgatcttcat taagctgctg cacctgggca agcgacggga tcatcagccg
    17281 tgcattttca gcatcctgcc gcgccacact ggcaaacgcc accgctgcca tttgacgggt
    17341 aaaatcggtc gctccggttg tacgcgcgaa ggtcagtacc gtattagggt tgttcgccag
    17401 tgaaatgatt gccgaggcga tagtctggta atcggcaggc atctgccctg ccagcactgt
    17461 taccaggcca gtgttacccg ctttcatcgc cagacggata cgctctaaat acgccagcgg
    17521 atcttgttta cctgacgcac gccagacgct aaataacttg tcacaggcgt taggctggct
    17581 cttgccggtt agccacagct ctttcgcccc ttgccaggct tcttcactct gcccggtgtt
    17641 ccatttcgca tagtagtaat tacattgcgc ttcggtagtt ccgggctttt ccgggctaaa
    17701 ggctaacaag ccacgccagt cttcacgccg cgccagttca ttgacgaaac gagattgcag
    17761 cgtgcgagcg ggaggaagcg tggggttagc gcgaacaaag ttagtgaccg tcaccgccgg
    17821 ttgattcatc agatcatcgg tgatctggcg gtattccagg tagggataaa gcggataatc
    17881 cttcagtcca ggcatcattt gttcgaccac atccatttgt cgattatccc aggcctgctt
    17941 gatttgcgcg taacggctac gctgctcatc cagtgagtcg gctcgcgcca cgctgctgac
    18001 cgtcagcaga cagacaccgg cagccaacag ccgccaggta acttgtttgg ctttttccac
    18061 aagcgcatcc tctaagtgta aatacatcaa tgcagcgtca tgccgcgtaa tagatttatg
    18121 ctaaccagtc attgccgttt acgccacgtt acggacactt ttttactttt actgcgaagg
    18181 tgatcgacca actgggatta ggcggggaaa aaggctacac ttcgcctttg aaaactcatc
    18241 atcacgataa acatagaggc gaagtccaac gtggctcaat tcgtttatac catgcatcgt
    18301 gtcggcaaag ttgttccgcc gaaacgtcat attttgaaaa acatctctct gagtttcttc
    18361 cctggggcaa aaattggtgt cctgggtctg aacggcgcgg gtaagtctac cctgctgcgc
    18421 attatggcgg gcattgataa agacatcgaa ggtgaagcgc gtccgcagcc agacatcaag
    18481 attggttacc tgccgcagga accgcagctg aacccggaac acaccgtgcg tgagtccatt
    18541 gaagaagcgg tttctgaagt ggttaacgcc ctgaaacgcc tggatgaagt gtatgcgctg
    18601 tacgccgatc cggatgccga ttttgacaag ctggccgctg aacaaggccg tctggaagag
    18661 atcattcagg ctcacgacgg tcataacctg aacgtacagc tggagcgtgc ggcggatgcg
    18721 ctacgtctgc cggactggga cgcgaaaatc gctaacctct ccggtggtga gcgtcgtcgc
    18781 gtagcgttgt gccgcctgct gctggaaaaa ccagacatgc tgctgctcga cgaaccgacc
    18841 aaccacctgg atgccgaatc cgtggcctgg ctggaacgct tcctgcacga cttcgagggc
    18901 accgtggtgg cgattaccca cgaccgttac ttcctcgata acgttgcagg ctggatcctc
    18961 gaacttgacc gcggtgaagg tattccgtgg gaaggcaact actcctcctg gctggagcag
    19021 aaagatcagc gcctggcgca ggaagcttca caagaagcgg cgcgtcgtaa gtcgatcgag
    19081 aaagagctgg agtgggtacg tcagggaact aaaggccgcc agtcgaaagg taaagcacgt
    19141 ctggcacgct ttgaagagct gaacagcacc gaatatcaga aacgtaacga aaccaacgaa
    19201 ctgtttattc cacctggacc gcgtctgggc gataaagtgc tggaagtcag caacctgcgt
    19261 aaatcctacg gtgatcgcct gctgattgat gacctgagct tctcgatccc gaaaggggca
    19321 atcgtcggga tcatcggtcc gaacggcgcg ggtaaatcga ccctgttccg tatgatctct
    19381 ggtcaggaac agccggacag cggcaccatc actttaggtg aaacggtgaa actggcgtcg
    19441 gttgatcagt tccgtgactc aatggataac agcaaaaccg tttgggaaga agtttccggc
    19501 gggctggata ttatgaagat cggcaacacc gagatgccaa gccgcgccta cgttggccgc
    19561 tttaacttta aaggggttga tcagggtaaa cgcgttggtg aactttccgg tggtgagcgc
    19621 ggtcgtctgc atctggcgaa gctgctgcag gttggcggca acatgctgct gctcgacgaa
    19681 ccaaccaacg acctggatat cgaaaccctg cgcgcgctgg aaaacgccct gctggagttc
    19741 ccgggctgtg cgatggttat ctcgcacgac cgttggttcc tcgaccgtat cgccacgcac
    19801 atcctggact accaggatga aggtaaagtt gagttcttcg aaggtaactt tactgagtac
    19861 gaagagtaca agaaacgcac gctgggcgca gacgcactgg agccgaagcg tatcaagtac
    19921 aagcgtattg cgaagtaatg cgtaaaatgc cggatgcggc gtgaacgcct tatccggcct
    19981 acaaaccaag aaatttcaat acgttgtgaa tctccaggcc tgataagacg cggtaagcgt
    20041 cgcatcaggc attgagcacc gaatgccgga tacggcgtga acgccttttc cggcctacaa
    20101 cagccttagt atccgcaatt tcgtagcggg tcacccgtgc aggttttttc aaaccttgtt
    20161 taatctcgac agcctcttcc agagaggttt tcaactcatc aaaaaactta ttttatacct
    20221 cgcctttgag ccgtttcatc gcggttatct ctgctccccc atcatctccc gcaccagctc
    20281 cacgcagcgc aggaaacggc tgtcataatc gtcctcttca acccgcacga attcgatatt
    20341 gttctcttcc agcatctcca ccagcaagtt ctggaactct ttgcgatcca ccgaactgcc
    20401 gaggctgcgt aaaccatccg ccacccacgg cgtgttgttc tccagcagga tcaccagatc
    20461 gaaacggtat tcatcaatca acgcctgtac gaacggatgc tcacgccctt cgtacttttt
    20521 gcagaacgcc tgggtagtga caaaatcagt gtcaataaac gccactttat tggcatattt
    20581 cactgcaaaa tcaatgtatt gtgcgtggcc cagcgcgatt ttatcgtaat cggaatactg
    20641 caatgcgatc tcatcaccgc cgaggtgtga aaagacataa tcgcgaccat attcccacgc
    20701 actggtggta ttgaagatat tggcaagttt gtttaccagg gtggatttac cgctcgactc
    20761 gccaccaagg atcgccacgg tacgtacaaa gaacggcttc acttccgtag gaatatattc
    20821 ccagtagcgg aacgggtttt cgcggatctg cgcaccgctg atactcataa aggtacgttt
    20881 cggatcgacc agcaccgtat cgatccccag atgttccata tactgtggcg catcggcttc
    20941 ttccgaggtg tagatcaggt ccggctgaat ccccttttca gccataaact ttttgatgcc
    21001 gttgctccac acatcccagc cgtgcggata cggctccatg ccctcttcgt tgaaagcatg
    21061 aatgcgaata tttttctgat atttaaaagt ttgtaataac cagcgcagac gatccggcac
    21121 cgtgggctgc tgcgacatgg cgctgtcttc aaacaacgcg cggtcgcggg tatcgtcaaa
    21181 acccataatg atatgcagct cgtcaacctg gctacaggcg cgctggataa ggtagatatg
    21241 tccggtatgc agcgggtaga acttaccgaa tacgacacca atggttttct tctgtcgggg
    21301 aaattcaagc cccaaaaaac ggtgcaacgc ctccagcttt tgcgcgctgg ggcttttaat
    21361 tttggcattc agtaactggc ttaaataccc tttggtcata ccgctggcat cagctacctg
    21421 ctgtagcgtg cagccctgtt gcttgatggc agttttcagg taatcaaatg acgacatatc
    21481 tccctccgta tctctcatta taagtcgtcg aacacgctaa gcgcgtcgga gagtttttta
    21541 acgccaaaaa tctgcatccc ttccggcgct tttttcggta cgttagccgc cggaacaatc
    21601 gcccggcgaa aaccgtgttt cgccgcttct gaaattcgtt cctgaccgct gggcaccggg
    21661 cggatctccc ctgccagccc gacttcacca aacaccacca gatcctgtgg cagcggtctg
    21721 tcacgcaggc tggaaaccat cgccagcagt aacgctaaat cggcactggt ttcggttacc
    21781 ttcacgccgc cgaccacgtt cacaaataca tcctgatcgg ccatttgcag accaccgtga
    21841 cggtgcaaga cagccagcag gattgccaga cggttttgtt ccagccccac tgccacgcgg
    21901 cgcggattcg ccatcatcga gtgatcgacc agcgcctgaa tctccaccag caacggacgc
    21961 gttccttccc acaccaccat cacggagctg ccggaggtca cttcatctcc gcgacttaag
    22021 aaaattgccg aagggttgct gacttcacgc agcccctgtt cggtcatcgc gaagacgccc
    22081 agctcattca ccgcgccgaa gcggttttta tggctgcgca aggtgcgaaa acgggagtcg
    22141 gcatcaccat ccagaagcac cgaacagtcg atacagtgtt ccagcacttt agggccagcc
    22201 agcgagccat ctttggttac gtgcccaacc atgacaatcg ccacaccgcg cgttttggcg
    22261 aagcgcgtca gataagccgc cgtttcacgc acctgcgcca cgctaccagg cgatgattgt
    22321 acatccgcca tatgcatcac ctggatcgag tcaattacca tcagtttcgg ttgctcttct
    22381 tcggcaatca ggcagatctg ttcgatgctg gtttccgaca acatattgag attgtcagtc
    22441 ggcaggccaa ggcgatgagc gcgcattgcc acctgttgca gcgactcttc gccagtgaca
    22501 tacagcgttt tcatctgctg ggccagtttg cacagcgttt gcagtagcag cgtggatttc
    22561 cccgcacctg ggttaccgcc aatcagaatg gcgcttcccg gcaccacgcc gccgcctagt
    22621 acgcggtcga actctttaaa tccggtggaa aaacgcggca gctcttcaag gctgatatcg
    22681 gagagtttct ggacttttgc caccccggcg ctaccggcat agccgctgag acgctcgtta
    22741 cgcgccaccg ttggcgacgc agcaagacgc acctcggtga tggtgttcca ggcatgacag
    22801 gcactgcact gcccctgcca gcgcggataa tcggccccgc attcattaca aacaaaggcg
    22861 cgttttggag cttttgccac ggtgtacctc gttaatgctg tgccgcccgc aggatggcgg
    22921 gcgagcaatt acttctgatt caggctgcca gagaggatgc agaatacccc catcaggtca
    22981 gcgtgacgga tggtgacttc cgccttttca ttcacttttg gcttggcatg gtaggcaatc
    23041 cccagccctg ctgctttgat catcggcagg tcattagctc catcgccaat cgccacggtc
    23101 tgcgccagcg ggatttcata ctcctgcgcg aggcgagtca aagttttcgc tttgtactgc
    23161 gcgtctacga tgtcgccaat cacattgccg gtaaatttac cgtccatgat ctccagttca
    23221 ttggctacca cggcggtcag gcgcagcttg tcgcgcagat attcagcaaa gaaagtaaag
    23281 ccgccggagg caatcgccac tttccagccc agcgtttcca gcttgagcac cagttgcgtt
    23341 aagcctggca tcagcggcag attttcacgc acctgttgca gaatattggc gtcagcgcct
    23401 ttcagcgtcg ccacacggct gcgcaggctg gcggtaaaat cgagttcgcc gcgcatcgcc
    23461 cgttcggtta cttccgccac catctcgccc gttccggcca gtttggcaat ttcatcaata
    23521 cattcaatct ggatggcggt ggaatccatg tccatcacca gcaaacccgg cgtgcgcagg
    23581 tgcgggattt tccccagcgg ggcgacatcc agttgcgctt catgggccag gcgcgtagcc
    23641 cgtgcggtga gtgaacctgc cagacgaatc acctgataat cttccacgca ccaggcggca
    23701 acaatcacca tcgccgcacc cagtttgctc tggtattggg tcagacgctg tttatccagc
    23761 ccacgaccat acagcagcca gccgctacga cctgcgtggt aatccagtgg catcacttca
    23821 tcaccactta atgaaagagg cagacccggc cataaagaga catcttcagg caggtcgcac
    23881 caggtaatgt taggcattaa ggctcctgta aaatcgttcg aagcagggaa aataacgcat
    23941 gaggctacct tgtatccatt gcttctggca acattaagtc tcaaattttc aaagggtgga
    24001 agatggctcg cacaaaactg aaattccggc tgcatcgggc agtgattgtc ctgttctgtc
    24061 ttgccttgtt agtggcactg atgcaggggg cgtcatggtt tagtcaaaac caccagcgac
    24121 agcgtaatcc acagctggaa gaactggccc gcaccctggc gcgtcaggtg acgctgaacg
    24181 ttgcaccgct gatgcgtacc gactcaccag atgaaaaacg cattcaggcg atcctcgatc
    24241 agttaacgga tgaaagccgc atcctcgacg cgggtgtgta tgacgaacaa ggcgatctta
    24301 tcgcccgttc aggcgaaagc gtcgaagtgc gcgaccggct ggcgctcgac ggtaaaaaag
    24361 caggcggcta ttttaaccag cagattgtcg agccaattgc gggtaaaaac ggaccgctcg
    24421 gctatctgcg cctgacactc gacacccata cgctcgccac cgaagcccaa caggtggata
    24481 acaccactaa cattttacgc ctgatgttgc tgctctcact ggcaatcggc gtagtgctga
    24541 cccgcacgct gctacagggt aaacgcaccc gctggcagca atcgcccttc ctgttaaccg
    24601 ccagcaaacc ggtgccggaa gaggaagaaa gcgagaaaaa agagtgaccc attactacaa
    24661 gaaaggaaat cgttatgtcc acattacgcc tgctcatctc tgactcttac gacccgtggt
    24721 ttaacctggc ggtggaagag tgtatttttc gccaaatgcc cgccacgcag cgcgttctgt
    24781 ttctctggcg caatgccgac acggtagtga ttggtcgcgc gcagaacccg tggaaagagt
    24841 gtaatacccg gcggatggaa gaagataacg tccgcctggc gcggcgcagt agcggtggcg
    24901 gcgcggtgtt ccacgatctc ggcaatacct gctttacctt tatggctggc aagccggagt
    24961 acgataaaac catctctacg tcgattgtgc tcaatgcgct gaacgcgctc ggcgtcagcg
    25021 ccgaagcgtc cggacgtaac gatctggtgg tgaaaacagt cgaaggcgac cgcaaagtct
    25081 caggctcggc ctatcgcgaa accaaagatc gtggcttcca ccacggcact ttgctgctca
    25141 atgccgacct tagccgcctg gcaaactatc tcaatccgga taaaaagaaa ctggcggcga
    25201 aaggcattac ctcagtgcgt tcccgcgtga ccaacctcac cgagctgctg ccggggatca
    25261 cccatgagca ggtttgcgag gccataacca aggccttttt cgcccattat ggcgagcgtg
    25321 tagaagcgga aatcatctcc ccggacaaaa cgccagactt gccaaacttc gccgaaacct
    25381 ttgcccgtca gagtagctgg gaatggaact tcggtcaggc tccggcattc tcgcatctgc
    25441 tggatgaacg ctttagctgg ggcggcgtgg aactgcattt cgacgttgaa aaaggccata
    25501 tcacccgcgc ccaggtgttt accgacagcc tcaaccccgc gccgctggaa gccctcgccg
    25561 ggcgactgca aggctgcctg taccgcgcgg atatgctgca acaagagtgc gaagcgctgt
    25621 tggttgactt cccggaccag gaaaaagagc tacgggagtt gtcgacgtgg atagcggggg
    25681 cggtaaggta actcgctgat taaaatacca aaggaaggga atatgatcga tctggaaaac
    25741 caggagcggg agattatcaa tttaatgtta agccagcgta tttcctggct agccgccgtg
    25801 cgtatcaggc ataaattatc acttgctgaa gtaagtaaaa tgttaggtat ctcgattaat
    25861 tctctaaaat agatcgaaaa gacggaacgg cttagctcaa acatcaaaag caaaatggga
    25921 gaaatttatg gttgtccacc agagttgctg atatgccctt cctggatgac ggctgaacat
    25981 aaatgaatag attcgtgaat agatacttga atgtatttta ttttttatcc atatgaaaaa
    26041 taatagtaaa tctataaatc agttgctatt gatgaataga tactaaaaag ccggagcagt
    26101 cccccactcc ggcttaacac gacaatcgcc tttcagcgaa acacaattac tctttatcgc
    26161 ccagcagaac ggattccagt gcgattttga tcatgtcgtt gaaggtggtc tgacgctcag
    26221 cggcagtggt ctgctcgtga gtgcggatgt ggtcagacac agtgcagatg gtcagggctt
    26281 tcgcgccaaa ttctgcagcg acgccgtaga taccagccgc ttccatttcc acgccgagga
    26341 tgccgtattt ttccatcacg tcgaacattt cgccgtccgg agagtagaac aggtcagcgg
    26401 agaacaggtt accaacgcga gcatcaatgc ccagtgcttt agccgcgtct accgcgttac
    26461 gcaccatgtc aaagtcagcg atagcggcaa agtcatgatc tttaaaacgg atgcggttaa
    26521 ctttggaatc ggtgcaggca cccataccga taacgacgtc gcgcagtttt acgtgcggca
    26581 gaactgcgcc acaggaaccc acgcggataa ttttcttcac gccgaaatcg gtgatcagtt
    26641 ctttggtgta gatggagcag gacgggatac ccataccgtg acccattacg gaaattttgc
    26701 ggcctttgta agtaccggtg aagcccagca taccgcgaac gttgttcact tcacgggcat
    26761 cttcaaggaa agtttcagca atatacttcg cacgcagcgg gtcgcctggc atcaaaacta
    26821 cgtcagcgaa atcgcccatt tctgcattaa tgtgtggggt agccattgtt ttatccttat
    26881 caacatgttt tcaccctctc cctgagggag agggccgggg tgagggcatc aagccgcacc
    26941 cattatcaga acatggcttt gccatattcc atatcagaag taccaaaata ttttgccaga
    27001 gtctggccga tatccgcgaa ggtttcacgg tgacccagtg agcccggttt tactttcggg
    27061 ccgtaaacca gtaccggaat gtgttcacgc gtgtggtcag taccggtcca ggtcggatca
    27121 caaccgtggt cagcagtgag gatcaggatg tcgtcatcgc gcagcagaga catcagctcc
    27181 ggcagacggc ggtcgaacag ctccagaccc acagcataac cggcgacgtc gcgacggtgg
    27241 ccccaggaag agtcgaagtc aacgaagttg gtgaagacga tggtgttatc acccgcttct
    27301 ttcatctctt tgatggtggc gtcaaacagc gcgtccaggc cagttgcttt cactttcttg
    27361 gtgataccgc agttggcgta gatgtctgca attttaccga cagaaaccac ctgaccgtgt
    27421 ttttcatcaa ccagtttctg cagcacggtc ggtgctggcg gctcaacagc caggtcgtga
    27481 cggttaccgg tacgctggaa gttaccggct ttgtcaccga taaacggacg agcgataaca
    27541 cgaccgatgt tgtagccgcc gttggtcagc tcttcacggg cgatttcgca cagttcgtag
    27601 agtttatcca ggccaaaggt ttcttcgtgg caggcaatct ggaacacaga gtcagcggag
    27661 gtatagaaaa tcggcttgcc ggttttcatg tgctcttcgc ccagctgatc cagaatgacc
    27721 gtaccggaag agtggcagtt accgaggtaa cccggcagat tagcgcgttc aaccagttta
    27781 tccagcagct cttgcgggaa gctgttttcg tgatcggaga aatatcccca ctcaaataga
    27841 accgggacac cggctatttc ccagtgacca gacggggtat ctttaccgga tgacatttcg
    27901 tgcgcccatg cgtacgcgcc gataacttca gcgttgccgt ccattcccgc cggaatgaaa
    27961 ccggtagaac cttcgtgtgc tttcgccagc cccagacggg tcagatttgg cagattgagc
    28021 ggacctttac gaccgttatc agcttcgcct ttggcacaag cttctgcgat atgacccagg
    28081 gtgtcagccc cgacgtcacc aaagcgttct gcatcttctg tagcgccgat gccgaatgag
    28141 tccagcacca taataaatgc acgtttcata tgttctccgt actcagtgct tcaaatttaa
    28201 atgatcagat cagtataccg ttattcgctg atacggcgat agacagttgg tgtgctttcc
    28261 ggtgctttat cggcaagttt aattgccgct ttcaccgctt tcgccgcttc ctgccagctg
    28321 ttttcgtctt tcgcgtggat aaccgccagc ggacgctgac cgtctacctg gtcgcccaaa
    28381 cgcgccatat cagtaaagcc gacgctgtaa tcaatggtgt cagatgcctg acgacgaccg
    28441 ccgcccattg caaccactgc catccccagc gcgcgggtat ccatttcact gacaaaacct
    28501 tcggtatcag catagactgc tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc
    28561 tcaacgaagt cggttgggcc tttttgtgcc gctaccatac gaccaaagac ttctgccgct
    28621 ttaccgttgt ccagcactgc ttgcaatttc gcgcgcgctt cggcgtcatc tttcgccagt
    28681 ttgccggaga taagcatctc cacgcacagc gccatcgtga catcaaacag acgcgggtta
    28741 cgatactcac ccgtcaggaa ctgcaccgct tcacgaactt caaccgcgtt acctgcactg
    28801 gaggccagta cctgattcat gtcggtgagc agcgcggtgg tgcgcacgcc agcgccgtta
    28861 gccacgccaa caatcgcttc ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg
    28921 ccgctaccca ctttcacgtc catcaccagc gcgtccagac cttccgcaag tttcttcgcc
    28981 agaatagagg cggtgatcag cgggatggag tccacggttg cggtaatatc acgggtcgcg
    29041 tagaaacgtt tatccgccgg agccagtgag ctggtctgac cgataatcgc cacgccgacg
    29101 tctttaataa tttcgcggaa acggttgtca tccgggaaaa tgtcgaagcc agggatggat
    29161 tccagtttgt cgagcgtacc gccagtatga ccgaggccgc gaccagagat catcggaata
    29221 tagccgccgc aggctgcgac catcggcccc aacatcagcg aagtcacatc gccgacgcca
    29281 ccagtggaat gtttatcaac aatcgggcca ttcagatgca ggcttttcca gtcgagaacg
    29341 gttcctgaat ctcgcatcgc catggtcagc gagacacgct caggcattgt catatcgtgg
    29401 aagaaaatgg tcatcgcgag ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaatg
    29461 ccgttgataa agaaacgaat ttcttcatcg ctcagtgcat gaccatcacg ttttttacga
    29521 ataatttctt gtgcgagaaa caaggtaacc tcccgaggaa aagagtatga ggcggctttt
    29581 gccggatgcg tctacgcctt atccggccta cgggtagata cgtctcgtag gcctgataag
    29641 cgcagcgcat caggcgtaaa gcatcttact tagtagctgc tggcgctctt accgtcaccg
    29701 tggcccagcg ctttcaacag gcttgccagc aggctggaag caccaaagcg gtagtgacgc
    29761 gcatctgccc agtcagcacc gaacagttca tctgcgatgg cgagatattt ctgcgcatct
    29821 tccgcagtac gcacgccgcc cgctggtttg aaaccaacgg ttttttctac gcccatatca
    29881 cggatcactt ccatcatgat gcgcgcgctt tccggcgtcg cgttcacagc cactttaccg
    29941 gtagaggttt tgatgaagtc cgcacccgct ttgatggaga tttcagacgc tttacggatc
    30001 agcgcttcgt ctttcagttc gccggtttcg atgatcactt tcagcagtac attcgctgcc
    30061 gcgcaagcct ctttacaggc tttcaccagg tcaaaaccaa cctgctcgtt acccgccatc
    30121 agcgcgcggt acgggaacac aacgtcaact tcatcagcac cgtaggcgat tgccgcacgg
    30181 gtttctgcca gcgcgatttc gatgtcatcg ttaccgtgtg ggaagttggt taccgtagcg
    30241 attcggattt ccggggtgcc ctgctctttc agcgttttgc gagcaatcgg gataaagcga
    30301 ggatagatac agatagcggc ggtattgccg accggggttt tggcctgatg acacagagca
    30361 attactttct cgtcggtgtc gtcgtcattc agggtggtca ggtccatcaa tttcagtgca
    30421 cgcaggctgc ttgctttcag atcagtcatt tcattctcct ggcttgtcgc caataaaatt
    30481 caccttgcga gtttgttagt attctaacat ctactccgca acacacttcg atatacatca
    30541 caattaagga aatctactta caagtttgca tcactgtaat gcgatctggt tcaaataatt
    30601 cactttcaaa tgaatgcgtc agtggtggca aacgcatcag gatcaaagtg aacatcacga
    30661 aacttcttac aatggcgcat caccttcggc ataaaaggaa aagaagatgc ccacttctca
    30721 tgaaaatgca ctgcaacaac gttgccagca aattgtcacc agcccagtgc ttagcccgga
    30781 gcagaagcgc cattttctgg cactggaagc agaaaacaat ctgccttatc cgcagcttcc
    30841 tgccgaagcc cgccgcgcgc tggatgaggg tgtaatctgc gatatgtttg aaggtcatgc
    30901 gccgtacaaa ccgcgctatg tcttacccga ttacgcccgt tttctggcga acggttccga
    30961 atggctggag ctggaaggcg cgaaagatct tgatgacgca ctctctctgc tgaccattct
    31021 ttaccaccac gtaccgtcgg tcacatcgat gccggtctac ctggggcaac tggatgcgtt
    31081 gttgcaacca tatgttagaa ttctaacaca agacgagatc gatgttcgaa taaaacgttt
    31141 ctggcgttac ctcgacagaa ccctgccaga cgcctttatg cacgccaata tcggcccgtc
    31201 tgattcgccc attacccgcg cgatcttacg tgcagacgca gagttgaagc aggtttcacc
    31261 gaacctgacc tttatctacg atcctgaaat cacccctgat gacctgctgc tggaagtggc
    31321 gaagaacatc tgtgaatgta gcaaaccgca catcgccaac ggtccggtgc atgataaaat
    31381 tttcacaaaa gggggctacg ggattgtgag ctgttacaac tcactgccgc tggcgggtgg
    31441 tggcagcacg ctggtacgcc ttaacctgaa agccattgcc gagcgcagcg aatcgctgga
    31501 tgacttcttt acgcgcactc taccgcacta ctgccagcag cagatcgcca tcatcgatgc
    31561 gcggtgtgaa ttcctctatc aacaatcaca cttctttgag aatagcttcc tggtgaaaga
    31621 agggctgatt aaccctgaac gttttgtgcc aatgtttggc atgtatgggc tggcggaagc
    31681 ggttaacttg ctgtgtaaga aagaagggat tgccgcgcgc tacggtaaag aagccgccgc
    31741 aaatgaagtg ggttatcgca tcagcgcgca actggcggag tttgtcgcca atacccccgt
    31801 gaaatatggc tggcaaaaac gcgccatgtt acacgcacag tcggggagca gttccgatat
    31861 cggcaccacg ccgggcgcgc gtttgccgta tggtgatgag ccagatccga tcacccatct
    31921 gcaaactgtc gccccgcatc atgcttatta ttattccggc atcagcgaca ttctgacgct
    31981 cgacgaaacc atcaaacgta atccgcaggc gctggtacag ctttgcctcg gtgcctttaa
    32041 agccggaatg cgtgaattta ctgccaatgt cagcggtaac gatctggttc gcgttaccgg
    32101 ttatatggtg cgtttgtcgg atttagaaaa atatcgcgcc gaaggttcac gcaccaacac
    32161 cacctggctg ggcgaagaag ccgcacgcaa cactcgtatt ctggaacgcc agccgcgcgt
    32221 gataagccat gaacagcaga tgcgctttag tcagtaagat tatccccttc tcctgcgttg
    32281 acgggccagg cagtcgtctg gctctgtttt tgcagggctg caatctgcgc tgcaaaaact
    32341 gtcacaatcc gtggacgatg ggacgttgca atgactgtgg ggagtgcgtg ccacagtgtc
    32401 cgcatcaggc gttgcagatt gttgacggca aagtggtgtg gaacgctgtg gtttgcgagc
    32461 agtgtgatac ctgcctgaag aggtgtccgc aacatgccac gccaatggcg caatccatga
    32521 gcgtggacga agtgcttagc catgtccgca aagcggtgct gtttattgaa gggataacgg
    32581 taagcggcgg cgaagccacg acccaactgc cgtttgtagt ggcgctgttt actgctatca
    32641 aaaacgatcc gcaactgcgc cacctcacct gcctggtgga cagtaacggc atgttgagcg
    32701 aaaccggctg ggaaaaatta ctcccggtgt gtgacggcgc aatgctcgat ctcaaagcgt
    32761 gggggagcga atgtcatcaa cacttaaccg gacgcgataa tcagcagatt aagcgcagca
    32821 tctgtttgct tgcagagcgc ggcaagctgg cggaactgcg tttgctggta attccagacc
    32881 aggtggatta tttgcatcac atcgatgagc tggcgacgtt tatcaagaga cttggcgatg
    32941 ttccggttcg cctgaatgcg tttcatgccc acggcgtgta tggcgaggcg caaagctggg
    33001 cgagcgccac gccggaagac gttgagccgt tggctgatgc gttaaaggtg cgcggggtga
    33061 gccggttgat atttccggcg ctctatttgt gacatgccgg atacggccta cggttcggca
    33121 cagacttgta ggcatgataa gacgcgacaa gcgtcgcatc aggcattatg cgccgactgc
    33181 cggatgcggc gtgaacgcct tatccggcct acggttcggc acagacttgt aggcatgata
    33241 agacgcgtca gcgtcgcatc aggcattgtg cgccaactgc cggatgcggc gtgaacgcct
    33301 tatccggcct acggtt
//
LOCUS       DAJTVK010000035        32615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000035 DAJTVK010000000
VERSION     DAJTVK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.035"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            424..744
                     /gene="cyaY"
                     /locus_tag="OO372_001697"
     CDS             424..744
                     /gene="cyaY"
                     /locus_tag="OO372_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6341221.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OO372_001698"
     CDS             complement(784..3330)
                     /gene="cyaA"
                     /locus_tag="OO372_001698"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6341222.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            3717..4658
                     /gene="hemC"
                     /locus_tag="OO372_001699"
     CDS             3717..4658
                     /gene="hemC"
                     /locus_tag="OO372_001699"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6341223.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4655..5395
                     /gene="hemD"
                     /locus_tag="OO372_001700"
     CDS             4655..5395
                     /gene="hemD"
                     /locus_tag="OO372_001700"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6341224.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            5417..6640
                     /gene="hemX"
                     /locus_tag="OO372_001701"
     CDS             5417..6640
                     /gene="hemX"
                     /locus_tag="OO372_001701"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6341225.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            6643..7839
                     /gene="hemY"
                     /locus_tag="OO372_001702"
     CDS             6643..7839
                     /gene="hemY"
                     /locus_tag="OO372_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6341226.1"
                     /translation="MLKVLLLFVLLIAGVVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            8501..8662
                     /locus_tag="OO372_001703"
     CDS             8501..8662
                     /locus_tag="OO372_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341227.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(8746..9981)
                     /locus_tag="OO372_001704"
     CDS             complement(8746..9981)
                     /locus_tag="OO372_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6341228.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(10128..10204)
                     /locus_tag="OO372_001705"
     tRNA            complement(10128..10204)
                     /locus_tag="OO372_001705"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10168..10170),aa:Pro,seq:tgg)
     gene            complement(10247..10333)
                     /locus_tag="OO372_001706"
     tRNA            complement(10247..10333)
                     /locus_tag="OO372_001706"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10297..10299),aa:Leu,seq:cag)
     gene            complement(10354..10429)
                     /locus_tag="OO372_001707"
     tRNA            complement(10354..10429)
                     /locus_tag="OO372_001707"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10394..10396),aa:His,seq:gtg)
     gene            complement(10488..10564)
                     /locus_tag="OO372_001708"
     tRNA            complement(10488..10564)
                     /locus_tag="OO372_001708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10528..10530),aa:Arg,seq:ccg)
     gene            complement(10665..12050)
                     /locus_tag="OO372_001709"
     CDS             complement(10665..12050)
                     /locus_tag="OO372_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6341229.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(12241..12981)
                     /gene="rffM"
                     /locus_tag="OO372_001710"
     CDS             complement(12241..12981)
                     /gene="rffM"
                     /locus_tag="OO372_001710"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6341230.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(12984..14336)
                     /gene="wzyE"
                     /locus_tag="OO372_001711"
     CDS             complement(12984..14336)
                     /gene="wzyE"
                     /locus_tag="OO372_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6341231.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(14333..15412)
                     /gene="rffT"
                     /locus_tag="OO372_001712"
                     /gene_synonym="wecF"
     CDS             complement(14333..15412)
                     /gene="rffT"
                     /locus_tag="OO372_001712"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6341232.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(15409..16659)
                     /gene="wzxE"
                     /locus_tag="OO372_001713"
     CDS             complement(15409..16659)
                     /gene="wzxE"
                     /locus_tag="OO372_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6341233.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(16661..17791)
                     /gene="rffA"
                     /locus_tag="OO372_001714"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(16661..17791)
                     /gene="rffA"
                     /locus_tag="OO372_001714"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6341234.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(17796..18470)
                     /gene="rffC"
                     /locus_tag="OO372_001715"
                     /gene_synonym="wecD"
     CDS             complement(17796..18470)
                     /gene="rffC"
                     /locus_tag="OO372_001715"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6341235.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(18448..19329)
                     /gene="rfbA"
                     /locus_tag="OO372_001716"
     CDS             complement(18448..19329)
                     /gene="rfbA"
                     /locus_tag="OO372_001716"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6341236.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(19348..20415)
                     /gene="rffG"
                     /locus_tag="OO372_001717"
     CDS             complement(19348..20415)
                     /gene="rffG"
                     /locus_tag="OO372_001717"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6341237.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(20415..21677)
                     /gene="wecC"
                     /locus_tag="OO372_001718"
     CDS             complement(20415..21677)
                     /gene="wecC"
                     /locus_tag="OO372_001718"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6341238.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(21674..22804)
                     /gene="wecB"
                     /locus_tag="OO372_001719"
     CDS             complement(21674..22804)
                     /gene="wecB"
                     /locus_tag="OO372_001719"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6341239.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(22860..23906)
                     /gene="wzzE"
                     /locus_tag="OO372_001720"
     CDS             complement(22860..23906)
                     /gene="wzzE"
                     /locus_tag="OO372_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6341240.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(23918..25021)
                     /gene="wecA"
                     /locus_tag="OO372_001721"
     CDS             complement(23918..25021)
                     /gene="wecA"
                     /locus_tag="OO372_001721"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6341241.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(25261..26520)
                     /gene="rho"
                     /locus_tag="OO372_001722"
     CDS             complement(25261..26520)
                     /gene="rho"
                     /locus_tag="OO372_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6341242.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(26605..26706)
                     /gene="rhoL"
                     /locus_tag="OO372_001723"
     CDS             complement(26605..26706)
                     /gene="rhoL"
                     /locus_tag="OO372_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6341243.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(26847..27176)
                     /gene="trxA"
                     /locus_tag="OO372_001724"
     CDS             complement(26847..27176)
                     /gene="trxA"
                     /locus_tag="OO372_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6341244.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            27307..28572
                     /gene="rhlB"
                     /locus_tag="OO372_001725"
     CDS             27307..28572
                     /gene="rhlB"
                     /locus_tag="OO372_001725"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6341245.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            28706..30190
                     /gene="gppA"
                     /locus_tag="OO372_001726"
     CDS             28706..30190
                     /gene="gppA"
                     /locus_tag="OO372_001726"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6341246.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(30237..32258)
                     /gene="rep"
                     /locus_tag="OO372_001727"
     CDS             complement(30237..32258)
                     /gene="rep"
                     /locus_tag="OO372_001727"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6341247.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         8226 a         8814 c         8183 g         7392 t
ORIGIN      
        1 tcatcgcagg agaagacgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
       61 gaggaaatgc tgcgagtata acgcttactc gtggggattt atgttttgct ggtagtttcg
      121 tgatcggcac ctcgcgccag gatggctcta tgcactatcg caatgtggtt tgtttgttgt
      181 cttttttagt tccgtcagcg tgaggatgct cgctcggtaa acctgatcat aatgtttacc
      241 agaaacaggg aaaggcgtgg tgtaactgcg ccctaatgaa gagacaaatc tttcgctaca
      301 cagagacatc attattccgg tgacggatga atcgtgccgg aacgcagccg cagaggtgca
      361 aactttgaag ccctgacccc atgccgctat actgccgcca tcacttaaaa acaggataca
      421 acaatgaacg acagtgaatt tcatcgcctg gctgatcaac tctggctgac cattgaagaa
      481 cgcctggacg actgggatgg cgacagcgat atcgactgcg aaatcaacgg cggcgtgctg
      541 accattacct ttgaaaacgg cagcaaaatc attatcaacc gccaggaacc gctgcaccag
      601 gtatggctgg caaccaaaca aggcggctac cattttgatc tgaaaggcga tgagtggatt
      661 tgcgatcgca gcggcgaaac cttctgggat ttgctggaac aggcggcgac gcagcaggcg
      721 ggtgaaacag tcagtttccg ctaagattgc atgccggata agcctcgctt tccggcacgt
      781 tcatcacgaa aaatattgct gtaatagcgg cgtatcgtga tcctgattgg caggcggcat
      841 gttaccgata gattttgtgc ggaacggaat cacctgttca cgaccatcaa ccttcacaat
      901 ctgatagaac tgcggcaggt tgaagttgat gaagcttgag ccgtaggtga agcggtcgtg
      961 cgatgacgag tagaagcgac tgacgtcacg caccagttcc tctttgctgc cttcgcagtg
     1021 gtgatacacc tcaacccggt tgctttcgtc aagaatgtag atattaaagc cattctcgtc
     1081 ttgcgtttct tcgaagaaga actggatgat cccttcacta gcaaagccgt ccaccaccgc
     1141 cggtaatttg acgtgattgg tttcaacctg cactgacagg ccgtgcagtt tgttatgcga
     1201 aatagcgcca taaaactcga tggcgttctc cagcttctgt accgatacat tcaggcgttc
     1261 gaagaacaag ccccaggttt gaccagaaac gcgcagcgcc ttgaaacgcc cggtttcctg
     1321 gcgggtgctg gaaaggcgca gttcaataca ctcagaaacc agttgctgca cgcgagtacg
     1381 gattaacccg cgcagatgct ggctataaca gaagacttcc acgctatctg gcggtgcggc
     1441 gtcctgatgc attttgccga gaatagtttt cagggcttcg atcatcgatt gctcgccgtt
     1501 gaagtgcagc gtacgcactt cgttccacga gttgcggtac agcaggtcaa cgctacctac
     1561 caggcaattt tgattctcgc caaagctgaa gacatccagc ttacggaaat caaaatgcac
     1621 cacctgattg cggaacgccg ctgtcgggtc atattccagg ttaacgataa tcgccagatg
     1681 gcggatctca cacgggctgt agagcgcctt cggtgtcggt gcaggtaagc gcagcgggaa
     1741 atggtgcgac acgtcggcga ccatctcctg caacttaggc aaatcgacaa tgccgttacc
     1801 tttaatatac aaacgggtgc gcgaggtcag cagaccgtta aaccatgccc acgccaccag
     1861 tttattcagg taacggttat attccagcgg ctgatggctg atgatcgact caatatttgg
     1921 cgcgcggtta tacagatacc aacctgaacg gttagcccgg cccggcggca cataaataaa
     1981 ggtcagattc ggttccgaga gatcgggtga aatctgcggg tttaccagcg tcactttacc
     2041 tggtaatgct tcaaacgcgg catacagctt acgcgtcagc acgccgatat cctgcggact
     2101 ggcggagacg ctaaggttat tgcgacgcgc aaagcggatc agattacggt agctctgcat
     2161 catcgcgtcg agcaactcgt tgtgcgcctc acgcacctga tcaatcttcc agttagcacg
     2221 gttatcgagc attgccagac gagcttcgtc ccaaccccac tcgctcacta actggctcaa
     2281 cactgcgcga cgccagccta cacaggcgcg ttcacggctg agtttttcgc acacttttaa
     2341 atagaagcag cgacgtacta aatccagacg ggtgaaatct tcaatcgccg tcaggtattc
     2401 agtaacacgc tccagcatca tgcagtatgg atcgaggcca aacgatacaa tctcgccgtc
     2461 gtgcaaacgc tgtttgatat ctttcgccag cagacgtggg ttcgggtatt cccaggaata
     2521 ggcttccagc agcagtgttt tcagcaccgc tttgtatggg gaatcgatac tcttgtagag
     2581 ctgccaaagg ctggcaccaa agtactcttc agcagaaagc gagcttaagc caccgagatc
     2641 cagccattca tttggcgtca gcacgccctg cgcgtaaagc gtcatcacat agtcgtcgta
     2701 atgctcttct tcgtcgcacg gcaccatatt ccacagaata cgcttaccgg cgagacgcac
     2761 ggcggtacga taaaattcgt caagcagcag tatatgctgg gtggagccac aatcttcgcc
     2821 ccccaggctg ccgctttcat tatgacgaaa gcggttttca tcaatcagga agaagctgac
     2881 ttccacaccc agcgaggcgg cccagctttc cagcaggctg catttacgtt gtagtaactg
     2941 gcgctcttcg ctatcgagcc aggattgatg acagacccag atatccaggt cagaggaaca
     3001 actttgccct acggacgagg tgctgcccat ggtgtataca ccagtaattg gaagctcacc
     3061 tttcggtgga tcctgtactg acattccacg atacagttca agctcgttca ggtagtggcg
     3121 ttgagtttca tcaggcgtgt aaaggcaaat gcctttggga acgttaccat caaggtaacc
     3181 cggcattagc ggatggtgat agtgcaacaa tgtcggcagt agactgtaga cctgttggaa
     3241 tgcaggcccc atagcagcaa gcgcgcgatc cacacgcaat tgatttatgg catccagtct
     3301 ctgtttcaga gtctcaatat agaggtacaa gacgtatcgc ctgatttgct acccgtcatg
     3361 actgtgattc cgccaacatc aacggtaaca cgcggcattc gggatatttc gtatgtcaaa
     3421 ggtaaccgtt accacttttc gcgcctggtt tttttagttt cacgacgaaa aaatggtcta
     3481 aaacgtgatc aatttaacac cttgctgatt gaccgtaaag aaagatgcgc tacatacaag
     3541 tgtagcaccg tttattctct gtaaattcct tattacgaca gcgtgaaacg tctgtcagga
     3601 tccactgcca gacctcattt tacggtttgc gcaggcgtct acgtttcacc acaacactga
     3661 catcactctg gcaaggatgt taggatggac cacggatgat aatgacggta acaagcatgt
     3721 tagacaatgt tttaagaatt gccacacgcc aaagcccact tgcactctgg caggcacact
     3781 atgtcaaaga caagttgatg gcgagccatc cgggcctggt cgttgaactg gtaccgatgg
     3841 tgacgcgcgg cgatgtgatt cttgatacgc cgctggcgaa agtaggcgga aaaggcttat
     3901 ttgtaaaaga gctggaagtc gcgctcctcg aaaatcgcgc cgatatcgcc gtacattcaa
     3961 tgaaagatgt gccggttgaa ttcccgcaag gtctgggact ggtcactatt tgtgagcgtg
     4021 aagatcctcg cgatgccttt gtgtccaata actatgacag tctggatgcg ttaccagcag
     4081 gcagtatcgt cgggacgtcc agtttacgtc gccagtgcca actggctgaa cgccgcccgg
     4141 atctgattat ccgctccctg cgcggcaacg tcggcactcg cctgagcaaa ctagataacg
     4201 gcgaatacga tgccatcatt cttgccgtag ccggactaaa acgtttaggt ctggagtccc
     4261 gcattcgcgc cgcgttacca cccgagattt ctcttccggc ggtaggacaa ggtgcagtcg
     4321 gtattgaatg ccgccttgat gatacgcgca ctcgcgagct gcttgccgcg ctgaatcacc
     4381 acgaaactgc actgcgcgtt accgcagaac gcgccatgaa tacccgtctc gaaggcggat
     4441 gtcaggtgcc aattggtagc tacgccgagc ttattgatgg cgaaatctgg ctgcgtgcgc
     4501 tggtcggcgc gccggacggt tcgcagatta ttcgcggtga acgccgcggt gcgccgcaaa
     4561 atgccaaaca aatggggatt tcgctggcag aagagctact gaataacggc gcgcgcgaga
     4621 tcctcgctga agtctataac ggagacgccc cggcatgagt atccttgtca cccgcccgtc
     4681 tcccgctgga gaagagttag tgagccgtct gcgcacactg gggcaggtgg cctggcattt
     4741 tccgctgatt gagttttctc cgggtcaaca attaccgcaa cttgctgatc aactggcagc
     4801 gctgggggag agcgatctgt tgtttgccct ctcgcaacac gcggttgctt ttgcccaatc
     4861 acagctacat cagcaagatc gtaaatggcc ccgactacct aattatttcg ccattggacg
     4921 caccaccgca ctggcactac ataccgtaag cggacagaag attctctacc cgcaggatcg
     4981 ggaaatcagc gaagtcttgc tacaattacc tgaattacaa aatattgcgg gcaaacgtgc
     5041 gctgatatta cgtggcaatg gcggtcgtga gctaattggg gataccctga cggcgcgcgg
     5101 tgctgaggtc actttttgtg aatgttatca acgatgcgca atccattacg atggtgcaga
     5161 agaagcgatg cgctggcaat cccgcgaggt gacgacggtc gttgttacca gcggtgaaat
     5221 gttgcagcaa ctctggtcgc tgatcccaca atggtatcgt gagcactggt tactacactg
     5281 tcgactattg gtcgtcagtg agcgtttggc gaaactcgcc cgggaactgg gctggcaaga
     5341 cattaaggtc gccgataacg ctgccaacga tgcgctttta cgggcattac aataactctc
     5401 ataacaggaa gccataatga cggaacaaga aaaaacctcc gccgtggttg aagagactag
     5461 ggaggccgtg gacaccacgt cacaacctgt cgcaacagaa aaaaagagta agaacaatac
     5521 cgcattgatt ctcagcgcgg cggctatcgc tattgctctg gcggcgggcg tcggtttgta
     5581 tggctggggt aaacaacagg ccgtcaatca gaccgccacc agcgatgccc tggctaacca
     5641 actgacggca ttgcaaaaag cccaggagag ccaaaaagcc gaactggaag gcattattaa
     5701 gcaacaggct acgcagcttg aacaggcgaa tcgtcagcaa gaaacgctgg caaaacagct
     5761 ggatgaagtc cagcaaaagg tcgccaccat ttccggcagc gatgctaaaa cctggctgct
     5821 ggcccaggcc gattttctgg tgaaactcgc cggacggaag ctgtggagcg atcaggacgt
     5881 cacgaccgct gcagcgttgc tgaaaagtgc agacgccagc ctggcggata tgaatgaccc
     5941 gagtctgatt accgttcgtc gggcaattac cgatgatatc gccagccttt ctgcagtatc
     6001 gcaggtggat tatgacggca tcatccttaa gcttaatcag ctttcaaatc aggtagataa
     6061 cctgcgtctg gccgataatg acagcgatgg ttcgccgatg gattcagacg gtgaagagct
     6121 ttccagttcc atcagcgaat ggcgtatcaa tctgcaaaaa agctggcaga actttatgga
     6181 caacttcatt acgattcgcc gtcgtgatga caccgccgta ccgctgttag cgccaaatca
     6241 ggatatctat ctgcgcgaaa atattcgctc tcgcctgctg gtcgcagcac aagctgtacc
     6301 gcgtcatcag gaagagactt atcgccaggc gctggagaac gtctccacct gggtacgtgc
     6361 ttactacgat actgatgatg ccaccaccaa agcgttcctc gacgaggtgg accagttaag
     6421 ccagcaaaat atctcgatgg atcttccgga aaccctgcaa agccaggcga tgctggaaaa
     6481 actgatgcag acccgcgtgc gtaacctgct ggcacaaccg gcagcgggga caacggaagc
     6541 taaacctgca cctgcacctg cacctgcacc tgcacctgca cctgcacctg cacctgcacc
     6601 gcaaggtgat actccggcag ccgcgccgca aggagaataa ccatgctaaa agtgttatta
     6661 ctctttgttt tgctgattgc gggggtagtg gttggcccga tgattgccgg tcatcagggt
     6721 tacgtgctga tccagactga caactacaat atcgaaacca gcgtcacggg cctggcgatt
     6781 attttgattc tggcgatggt agtgctgttt gccattgagt ggctactgcg gcggatcttc
     6841 cgcacaggcg cacacacccg tgggtggttt gtcggacgta agcgtcgccg tgcccgtaag
     6901 cagaccgaac aggcgctgct gaaactggcg gaaggcgatt atcagcaagt tgaaaagctg
     6961 atggcgaaaa atgccgatca cgcggaacaa ccggtggtga actatctact ggctgccgaa
     7021 gccgcgcaac aacgtggtga tgaagcacgc gccaaccaac atctggaacg cgcagcagag
     7081 ctggccggca atgacaccat tccggtagaa atcacccgtg tacgtctgca actggcccgt
     7141 aatgaaaacc atgctgcacg ccacggcgtg gataagctgc tggaagttac gccacgccat
     7201 ccggaagtat tacgtctggc ggaacaggcg tatatccgca caggtgcatg gagttctctg
     7261 ctggatatca tcccatcaat ggcgaaagcc catgttggtg atgaagaaca tcgtgcaatg
     7321 ctggaacaac aggcatggat tggcctgatg gatcaggcgc gtgccgataa cggtagtgaa
     7381 ggtttgcgta actggtggaa aaaccaaagc cggaaaacgc gtcatcaggt agcgttgcag
     7441 gtggcaatgg cggaacatct tattgaatgt gacgatcatg atactgccca gcaaattatc
     7501 atcgatggcc tgaaacgcca gtacgacgat cgcctactgc tgccgattcc tcgactgaaa
     7561 accaacaatc cggaacagct ggaaaaagtg ctgcgccagc agatcaaaaa cgtcggcgat
     7621 cgcccgctgt tgtggagcac actgggccag tcactgatga agcacggaga atggcaggaa
     7681 gcatcgctcg ccttccgcgc agcgctgaaa caacgtccgg acgcctacga ttacgcatgg
     7741 cttgccgacg cgctggacag actgcacaag ccggaagaag ctgcagcgat gcgtcgcgac
     7801 ggtttgatgt taacgttgca gaacaaccca ccacagtagt tccttctcac ccggaggcaa
     7861 gcacctccgg gacctttctg atgcataaaa aaacgcctgc tcttattacg gagcaggcgt
     7921 taaaacaggt ctgtatgaca acagtgggtg cttcactcaa cgttgtgtcc atggtgtctg
     7981 atgaggcata agcgacatct gtcagtggac gataagcacc gtaaacggct ctgcgtcatt
     8041 ccggagttta tgaggcacta aggcgaacat aagagatgga atgagcatct acccgtttat
     8101 tatgccacag agaatcggga aataacatcc cttaacactt gttatgagat aattctgtaa
     8161 tcctctttgc ttcctgagtg aatatttaac ctgagcttga tcctacacat acttattatg
     8221 aatgataaaa ttcattcaat taataacaca tatattaatt gccgttaaaa ctaaaaaagc
     8281 attaataatc aacgcaatat aataaacccg ccttacatat caactgcgcc agaggtagga
     8341 ttgaaaacgc tctcctgatt ttccaattca ttttctggat aaataaataa tttatttttg
     8401 tcactattat ttatgtaatc atcctgtcag ggagagggat ctcaattatc aatgcttaat
     8461 tacgtcatca ttttgatgac atgaaaccgg agcattttgc atggaatttt cgttttcacc
     8521 caaacttctt gttgttgctg tcgccgccgc tcttcctctc atggccagcg cagcagatac
     8581 cccgtcaact gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc
     8641 acagaacgct gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc
     8701 cgcatccggc aatcaaccgc aggcggccac aaaatggccg cttttttact tattcaccac
     8761 cagcaaatgt gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat
     8821 tgctttaaga tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt
     8881 cagccccggt ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccagca
     8941 cgcttttaac acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc
     9001 aaacacctgc tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc
     9061 cagccgatat tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac
     9121 aatcaccgag cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa
     9181 attggcaaac gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca
     9241 gtggtcgaat atcgccacca gaaattcacc aaattcatcg gggcgcaccg accattccgt
     9301 cagttccccc tgaatatcac caggcgcatg caatttcagt ccatcgcggg cagctgtttc
     9361 atcagccaga cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa
     9421 aaaatcataa acctgcaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt
     9481 atagtcgaca tgatgttttt gcagcagcgt cagggcacgc atcaccagct tatgcgttgg
     9541 tctgccacct ttggtcacgc gatattgatt gtggatctca gccggaccgt ccagcgataa
     9601 cccaacaaga aaatgatttt ctgccagaaa tgcacaccat ttatcatcga gcagcacgcc
     9661 gttagtctgg aagctgttac ttatcttcct gccagcaccg tatttcgcct gtaactttac
     9721 ggcacggcgg taaaaatcca gcccgagtaa cgttggttcg ccgccctgcc aggtaaaagc
     9781 cacttcgttt tgcggttcgc tggcagcgat atagtgacgg acatacgctt ccagcgtgtc
     9841 atcgtccata tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca
     9901 gtagtcacag ttcagattac aatcggaacc actcggtttc gccatcacat gaaaagcacg
     9961 cgttggaacc tgttgcagca tggtcactcc ttgcctgaag tcatccttca atacgttaag
    10021 ggcgaaatca taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga
    10081 cgaaatgcac agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag
    10141 aggattcgaa cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc
    10201 gccgaaatac tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg
    10261 ggacttgaac ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt
    10321 ccgccacctt cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca
    10381 cgacaactgg aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc
    10441 ttgttacgcg gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac
    10501 aggattcgaa cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg
    10561 gcgcttagcg ccgttgcggg gggataatac ggacttccca cccctctgtc tagtcccttt
    10621 ttaaataaat tgcgcgtttg gttacggttt gtgcgttttg ctgcttattc ctccagttta
    10681 tgcgctttcc cgtggcgatt aaggccaaaa actttataaa tcgccgtcac cgccagcata
    10741 aagatgatgc caacaaacag cgacatacgc gtatcttcat taaagtacat gccgatcaaa
    10801 acgcaaatca ggaatgccat tgttacgtag ttggcccacg ggaacaggat tgagcggaac
    10861 ggatggctgg caatcgccgc tttatgtgca cgccgaaaac gcagctggct tatcaatatc
    10921 acaaaccatg gcaccatccc cggaagcacg ctggcactgt agacgtagac aaacacacgc
    10981 tgcggattgg gaatgatgta gttcaggcat gagccaatta gcagaatagc aatagatact
    11041 gccacacccg caactggtac gccgtgacgg gaaactttcg ccatcgccgc cggtaactga
    11101 cggtttttcg ccagtgcgta gagcatacgt ccgcaactgt acatgccgct gttacagcca
    11161 gagagcgcag ccgtcagcac cacaaagttg ataatgcccg ccgctgcggt aataccgatt
    11221 ttggcaaaag tcagtacgaa cgggctgccg ttgctgccaa tttcattcca cgggaagatg
    11281 gtgacgataa cgaaaatcgc gcctacgtag aaaatcagga tccgccacag cactttgcct
    11341 acagcactgc gcagcgtcac ctgcggattc ttcgcttcac cggcagtaat gccaatcagc
    11401 tccacgccct ggtaggacgc caccacaata cacagcgcgg tcaggaaccc tttccagcca
    11461 cccgcaaaga aaccgccatg ctctgtgaga ttgctaaaac caatcgactg cccgccattg
    11521 ccaaagccaa agaaaatcac gcccaggcca atgacaatca tcacgataat cgtggtgact
    11581 ttgatcatcg cgaaccagaa ctcgatttcg ccgtacaacc gcaccgccgc cagattcgcc
    11641 aacgccacca gcgccaccgc gatcaatgca ggtatccact gcgccatctc cgggaaccag
    11701 aactggacat aaacgccaat ggcggtgatt tctgagatcc ccaccgccat ccacataaac
    11761 cagtaagacc aggcggtgag ataaccaaaa aacgggctca tataacgatg cgcataaacg
    11821 gcgaacgaac cggtaacagg ttcaaggaac aacatttcgc ccattgaacg catgatgaaa
    11881 aagacgaaca gcccggcgat gatataggcc aacaatacgg atggcccggc ccatttcagg
    11941 gtactggcgg cccccataaa caggccgacg ccaatagtgc cccccagggc gatgagttcg
    12001 atatgtcgag cttccagccc acgctgtagc tctggtttgt tatctgccat aaatcctcgt
    12061 gttgtgtttg catgctttcc ggtgttaccg gttatcgtta tgggtacatc gagtgttgcg
    12121 aatgttttcg taattcagga gaaatggcaa ataaagcatt aaaaatttga atgctttgtg
    12181 taataaaaaa gcagacaggc gacggagtga ccactccgtc gctttacaaa gagaggaaaa
    12241 tcagaggttg ccggtgtagt gccagcgtaa ataacgcagc aaacgaagct gacgcttaat
    12301 gcggctcggc tgcgaaagca ggcggtagag ccactccagt cccagcgttt gccagatttt
    12361 cggtgcgcgt tttacgtgac cggtgaaaac atcgtaagtc ccgccaacgc ccatatacag
    12421 cgcatctgga tgcaccagac ggcagtcgcg catgaagatc tcctgctttg gcgatcccat
    12481 cgcaacggtg acgatttgcg ctccgctggc atgaatgcgt tcaaacagcg cctgacgctg
    12541 ctcgggttta aaataaccat cctgactgcc aacgatattc acattccatt ggttgcgcag
    12601 tttagcttca gtttgcgcca gcacttcagg tttaccgccc acaagaaata ccggcgtccc
    12661 ttctttgcct gcgcgcgcca tcagctcttc ccagagatcg gcaccggcaa cgcgggaaac
    12721 ctgcgcctgc gggtactttt tacgtactga acgtacaacg ctgatgccat ccgcatattt
    12781 aaattcggca gcgttaatta actccctgac ctcggcgtta tcttcaatgg tcagcatttt
    12841 ttcagcatta atggcaacca gcgttccctg cttaagctgc ccgtcagcaa acagataatc
    12901 gagggcgtgc tgcatatcac gccaaccaat caactgtaag ccacgcagcg tataggtcgg
    12961 tgccgtggtg ttgttattca ttgttatcct tcaacctgcg tccggagcga tgattttgta
    13021 cgtttatgaa tgagtccggc gctttcaaaa agccagtaca gcagttttgc gatcatcaga
    13081 catgcgccga agaccacgat aaaaaagact acgcgtgaga caaacgaatc cagcccttca
    13141 cgcgccagca cgatcatatt gaagatcgcc ccaaagcaga aactgtgcaa tatcgcagcc
    13201 ttatagcgat taggctcgcg gttgcccagc tcatacagcc agtcgaacca tttgatgatc
    13261 agaccaacca cgatcgcccc gagcgggatg aacaacgcgc cgcccatcac caccagtgag
    13321 cctataagcg taggcgagat cgccagtccg gagtggttat tcagcacttc ccaggtaaag
    13381 tagttggctg agttcagcac catactcggg cgacccggcc acagccagga agggataaag
    13441 acatagaaat cgcggacaat tggagccagg ccctggaagt cgatgttgtc gtagttctgc
    13501 aacagcaacg ccagattctc ccacggggag aaggtgtcgc gagtgagata gagaaacgta
    13561 tagaacgctt catcgccgct cacattcatt ccatagcgtt ttagcgccag ccagaacatg
    13621 ccaacaatcc ccagcacgcc cgccgccgcc agcatccaca acgaaatcca gccgcgaata
    13681 atgccaataa acaggaagat agcgaatgcg atgatgatat tggcgcgagt gccgccgaca
    13741 atcatataag tcagcaagcc aaaggcgacc gtgctgacga ggaaaaacag ccacgctttg
    13801 ctgtcctggc gcagaaagta gaccaccagc atcgccggga tgaaaaagta aaagaagcgt
    13861 tttaacgcca cgccggagac ttcactggag aagatctgac tgtaggagtt aagccggaac
    13921 agcaaaaagc cgttgtgcat gaagaagatg ccgacgctta ccagcgcgat acccatcagg
    13981 atcacccacg taagattggt ctccacgcgg ttcatggtaa acagcggacg gcgcggtaca
    14041 tcagcaacgc gtttgcgtag gcgggttttg taggtgacat agtaaaccgc gtagaagcag
    14101 cccgcagaaa gcaacgcctg caacaagatt tctggcggcg cgacaccaac atcaaagcga
    14161 aataccagca cgctggtcag cgggaagccg aagaaaaagg tgagcaaaaa cagcaatgaa
    14221 aagaagacat tgaagttaaa gcgcacacgg cgaaactcaa accaggtcag cgtggcaata
    14281 aacagcgtgc agagcagcca gaccacaaac aggccactga attgcagcag actcatgcga
    14341 cctccccggc ggcaatagcc aacgcccgct gccagccttg tagatagtta gggctaaaga
    14401 aggcaatggt gtttttatcc accgacgcca actggcgctg cgcttcacgc acaatatcct
    14461 cgttgagatc gtcggtagta aacagcaccg gcaaatgctg ttccgtcata tcctgccaga
    14521 acggattttc ccggttaagc acacaaggaa tgcccgcctg aatcagtaag caaagcgtac
    14581 caatgccctg ctggcgggca aaaataaagt aaccaagatc gcactgacga agtagcgcca
    14641 gataggcgtc aaattccagt ttttcgctca aaatttgcag attttcttcg ctgaataact
    14701 ccagccccgc ctgacgaact tcctcaatgt acgcttcgtt attaggcgga tatcccatcg
    14761 gcaccaccac ttttaccgta tcgccaaatt gctgatgaac ggcgcgcaag gcagcaatat
    14821 ggtcattgct gcggtcaccg gagttcccca ccagaatggt cattttccct tcacgttgcc
    14881 gatcattcgc catcgtattg agagaagggt ccatccgcgt cgggaagtac agcagttcgc
    14941 cccgcacctt tggatgcgtt ttggcaaaaa agctcaaatc accgcgggtg gcaaatacac
    15001 agccgactcg cttttgcgcc aggcgacgta gcgggtaaaa aagcttatat ctcaagccac
    15061 tggaaagctc atacaggtct gccccccaga tatgccagaa aaactggctg ggcttaatgc
    15121 caccactcag cagagccagc cacagtgtgg gattgaactg accgtggaaa aaaaatcgct
    15181 gctgacggtt agcttttgct ttcgcgatga ccgcttccgc cagcgatttt ttcccaggga
    15241 aaaattgcac agaaagcgcc ggacagctat cacttaagcc gtcgtccttg ccaacaacca
    15301 taaactcgcg cgcgtgctcg ctcgtcgcgg ccagcgcgtc attgaaaaac cgcaaaacgg
    15361 ttcggttatg gtgagggata tccgatccca gtacgtgaat cagtacagtc atgcccgcct
    15421 acgccagagt aaaaacacgc cacaacaaag agaaaaatag acgatataag ttgccatata
    15481 tgcctgcgcc gcgcccagtg caccatgcgc agggattagc cagtgggcaa ataccatcaa
    15541 taaagtgaac tggctgactt ccgccagaat ataaaaccgc agtgacgctt tggcgatcac
    15601 cagataacca aagacataag cgcccacttt taacacatca cccactaact gccaggcaaa
    15661 gagatcgcgc atagcggtaa atttattcga caacagcaac cagatagcaa aatcacgcag
    15721 caaccagacg gtaaaactcg ccgccgccac tgccggtaag acgaatttca gcgatttaac
    15781 cacttcccgg gtgatatcgc gcttttccgt tagccgcgac aacgtgggca gcaaatatac
    15841 gctgaacgat gccgtaataa attgcaggta ggcatcggaa atactgctca ccccttgcca
    15901 gatccccact tcatcccagc tatactgcgc cgccagcagt ttacgcatca tgatgtaagc
    15961 aacaggcaag gtcaccgacg taatcaacgc catgagcgta aatttgctca actgccctgc
    16021 cagaccgtta tcccagctgg gtttcagata gcttaacggg atgacaccac gtttgatcaa
    16081 catgatggcg gcaggaatca ccaccagcgc gggaatcagc gccagaccca gcaacgcccc
    16141 ttcataaccg cccaaacggt aactgacgta gtacgcgaga acgccaatca agctgccgac
    16201 aatcagcgat aacgcattac ctgcggcatc gcgaaagcct ttcatcagcg ccagtaacag
    16261 gttgccccag gcgatcccca tttgcaccag cgccactaaa cgcaccagcc cctgatagtc
    16321 ggtattacca aacagtccct ggctgattgg cgcagctgcc agcacaaaaa ccagcgccat
    16381 cagcgtagag aaaccaagta ccatcgctga tgaagtgccg accacgcggc gcagctgttg
    16441 cggattatca tggtactggg caacgtattt ggttacaccg ttaaagatgc cagctccggc
    16501 aagcacgccg agcacggtaa tcaactggcg gaaatttgcc gccagcccaa gccccgccgg
    16561 accaaatgac acagccagca acttaccgac cagtaacccg gcaccaatct tgaccagtgt
    16621 actggccgcc gtccacaagg acgcttttgc caacgacata tcaggagaag tagttcaaca
    16681 aagtcgcaat taccgtacgc tgattgacgg gcgacaggtt gtagaacagc ggcaggcgca
    16741 gcaggcgctc gctctctttg gtggtgtagc gatcttcacc gtggaactca ccaaagcgtt
    16801 cccccgcagg gcaaccgtgc agcggaatgt aatggaacac cgccatgatt tccgcttctt
    16861 tcagaaagtt aatcaacgcg ctccggtcat caatatcccg cagtttaatg tagaacatat
    16921 gcgcgttctg cacgcagcca tcgggaatcg acggcagctc gatacgcccg gctttcgcca
    16981 gaggcgctaa cgcatcgtag tagttttgcc acagcgccag acgttgctgg ttgatacgat
    17041 ccgctgcttc cagttgcgcc cacaggtacg cagcttgcag atcggacatc aaatagctgg
    17101 agccaatatc gcgccaggta tatttatcga cctgaccacg gaagaactgg ctgcggttag
    17161 tgcccttttc acggatgatc tcggctcgtt cgattaacgc tttatcgtta atcagcgtcg
    17221 cgccgccttc accacccgcc gtatagtttt tggtttcatg gaagctaaag cagccaatat
    17281 gaccaatggt tcccagtgca cgccctttgt aagtggacat cacgccctga gcggcatctt
    17341 ctaccacaaa cagattatgc tttttcgcca acgccataat ggtgtccatt tcgcaggcca
    17401 cacccgcgta atggaccggc acgataacgc gcgttttgtc ggtgatcgcc gcttcaatca
    17461 gcgtttcgtc gatgttcatg gtgtccgggc gaacatccac aaaaacgatt tttgcgccac
    17521 gcagcacaaa ggcattggcg gtggagacaa aggtgtagct cggcatgatc acttcatcgc
    17581 caggctggat atcgagcagc agcgccgcca tctccagcga agcggtgcag gacggcgtca
    17641 gtaacacttt ggcgctgcca aaacgttgct ccagccactg ctggcagcga cgggtaaaac
    17701 cgccatcgcc acacagtttg ccgctaccca ttgccgactg catatagtcg agttcggttc
    17761 ccaccaccgg cggtgcgtta aatggaatca tgtgatcacc tgtataacca gtacgcggtg
    17821 ctttctacat tcgcaccact ttgtatgtat cgtttaagcg cggcggtgtt gcccatttgg
    17881 gtcgccaccc gcaaagttgt tttaccgcga gcatacgccc agtttagcgc cgtttgcatc
    17941 agctcagcac ctgcaccgcg tccagccagc aggccaattc gcgcatctgt cgcattgagt
    18001 tcccgtaaag agacataacc gcgaataccg ccggacgccg cacgtaaaat cagacattga
    18061 tgatcaaagg tgccgcgcac ggcattttca atccactgtg cataaaagcg tccgctggcg
    18121 tcaggcgcat accacggcgc acgaaaacgg ctttgcgcaa atgcggcgct ggctaactga
    18181 cgtaatgcgg gaatatcggt ctcttgtgcc actacagcac cactatcact gacattgttc
    18241 acgggtagtg ccaaatcaac ttcaccttct accagggaga atcccagctg ttgcagggca
    18301 tccagttcac ccgtatttga tgccgcaatt ttggcctgca cccgtgacca cggcgctaac
    18361 acgtctggcg tcaggagctg tgcttcagac gtaatgcgca cgatggcgct gttaacacca
    18421 aagaaggcgt tttcccaggt tagtggctca atactggcgc ggacgggcac gaagtaactc
    18481 cagcagatat tggccgtagc cagttttcgc taatgaactg gcagcacgct tcacaccctc
    18541 gtcatcgagc cagccgttac gccaggcgat ctcttccagg caggcaatct taaagccctg
    18601 gcgtttttct actgtctgca caaaggtact ggcttcaatc aggctgtcgt gagtgccagt
    18661 gtccagccag gcaaatccgc gcccgagcag ttcaacggtc aggttgcccg cctcgaggta
    18721 catctggttg atggaggtaa tctccagttc accacgctcc gacggcttca cctgctttgc
    18781 gtactccacg actttactgt cgtagaaata aagcccggtc accgcccagt ttgacttcgg
    18841 ctgttttggc ttttcttcca gcgagatagc gcggaaattg tcgtcaaact ccaccacgcc
    18901 aaagcgttcc gggtccatca cctgatagcc aaataccgtc gccccttccg tgcgcgccgc
    18961 aacatgacgc agcttcggac tgaagccctg accgaagaag atgttatcgc ccagcaccag
    19021 acaagaaggt tcaccgttga ggaaggtttc accgatgata aaggcctgcg ccagaccgtc
    19081 cgggctgggc tgttcggcgt attccagctg gataccgaac tcactaccat cgcccagcag
    19141 gcgctggaaa taacctttat cttccggcgt agtgatgatg agaatttcgc ggatatcggc
    19201 caacatcagc accgacagcg gatagtaaat cattggctta tcgtaaatcg gcaacagttg
    19261 cttcgatacg ccgcgcgtaa tcggatgcaa tcgggtgccg gaaccgcccg ccaggataat
    19321 acctttcatg tttgcctccg ctgaaaatta gcctttcaga cctaaacgct cgccctgata
    19381 gctgccgtcc tgcacctgct tccaccagct ttcattagcc agataccact gcaccgtttt
    19441 acgcattcca ctttcaaagg tttcctgtgg cagccagcca agttcacggg caattttcga
    19501 agcatcaatg gcataacgca gatcatgccc cggacggtca gcgacaaagg tgatcaagtc
    19561 acgatagtgc accacgccgt gcggcttgtt cggagccagt tcttccagca gctcgcaaat
    19621 ggtttccaca acatcgagat tcttacgctc gttgtgacca ccaatattat aggtttcacc
    19681 gactttcccg gtggtcgcca cgcaatacag cgcgcgggcg tgatcttcca catacagcca
    19741 gtcacggatt tgctgcccgt tgccatatac cggcagcggt ttacccgcca gcgcgttgag
    19801 gatcatcagc gggatcagtt tttccggaaa gtggtaagga ccgtagttat tcgagcagtt
    19861 ggtgataagc gtcggcagac cgtaggtccg cagccaggcg cgcaccagat ggtcgctgct
    19921 ggcttttgac gcggaataag ggctgctcgg cgcatacggc gtggtttcgg tgaagaaatc
    19981 atccgtcgag tgcaggtcac catatacttc gtcggtggag atatgatgaa aacggaacgc
    20041 tgatttttta tcttccgtca gcgtattcca gtaagcccgc gccgcttcaa gcaatgtata
    20101 agtcccgaca atgttggttt caataaacgc tgccgggccg tcaatagaac ggtcaacatg
    20161 gctttcggct gccagatgca tgacacagtc tggctgatgc tcagtgaata cgcgtgccag
    20221 ttctgcccga tcgcagatat caactttctc aaaggcaaag cgctcgcttt gcgcgaccgg
    20281 tgccagcgac atcaggtttc cggcgtaggt cagcttatcg accactacca ccgcgtcgct
    20341 cgtttcgttg atgatataac gcaccagcgc cgagccaata aacccggcac cacctgttat
    20401 cagaattttt ctcatcagcg ccagactcct ttggcatcga cgacatactg ctgatggaca
    20461 ttgtcgccat tgataacttt aaactgacta tgatcgacca gcatcaccag cacgtctgcc
    20521 gttgccagcg cctcgtcaag ctgcgccaga gtacaaagcc cggtcagttt tttcggcaac
    20581 tggtggatgt taggctcaac aaccagagtt tcgccgctat gccactgggc gatcagttca
    20641 gcgatttcca tcgccgggct ttcgcgcagg tcatcaatat tcggtttaaa cgccagacca
    20701 aagcaggcga ttttcagttc actggcgcgt ttatcggtag ccgccaggca atcagccacc
    20761 gccgctttca cctgatcgat aacccagaac ggtttgtgat cgttcacttc gcgcgcggta
    20821 cggataagcc gcgcctgctg ggggttctgt gccacgataa accacggatc aacagcaatg
    20881 cagtgaccgc ccacgccagg gccaggctga agaatattaa cgcgagggtg acgattcgcc
    20941 aggcgaatca gttcccagac gttaatcccc tgatcggcac aaatcagcga caattcatta
    21001 gcaaaagcga tattcacatc gcggaagctg ttttcggtga gcttacacat ttccgccgtc
    21061 cgcgagttag tgacgacaca ctcaccttcg aggaaaattt tgtacagttc gctggcgcgg
    21121 gtcgaacaaa ccggcgtcat accaccaatc acgcgatcgt ttttaatcag ctcgaccata
    21181 acctggcccg gtaacacgcg ttccgggcag taagcaatgt tgacgtccgc ctgctcgccc
    21241 acctgctgtg ggaaagtgag atccggacgc atctctgcta accattctgc catcttctcg
    21301 gttgacccca ccggcgaggt ggattcaagg atcaccagcg cgcctttttt cagcactggc
    21361 gcaatggagc gagcagccga ttcaacgtag gtcatatctg gctcatgatc gcctttaaac
    21421 ggcgtgggta cagcaatcag ccaggcatcc gcttcaactg gcgtcgtgct cgctcgtaaa
    21481 aaaccgcctt ctacggcagt ttttactacg ctcgccaaat caggctcgac gatatggatt
    21541 tcgccacgat tgatggtatc aaccgcgtgt tggttgatat cgacaccaat cacctgtttt
    21601 tgccgtgagg caaacgctgc tgctgtaggc agcccgatgt aacccagtcc gataacagaa
    21661 atggtcgcaa aactcatagt gatatccgat tattttttaa cgcttccaga atgcgagagc
    21721 atgcctgacc atcaccatac gggttatggg cgcggctcat agtttgatat tcgttttcgt
    21781 cttttaaaag acgcgtcact tcctcgacaa ttcgctgctt atccgtgcct accagacgca
    21841 ccgtacccgc cgtcaccgct tccggacgct cagtggtatc gcgcatcacc agcaccggtt
    21901 tccccagcga aggcgcttct tcctgaatgc cgcctgagtc ggtcaaaatc agccaggcgt
    21961 ggttcatcag ccagacaaac ggtaaatact cctggggatc gatcagaatg acatttttca
    22021 catgccccag aatgcgattg accggctctc tgacgttcgg gttgagatgc accggataga
    22081 caatctggat gtcctggtgc gtggtggcaa tgtctgccaa cgcgtggcag atttcttcaa
    22141 agccacgacc gaaactttca cgcctgtgac cggtcaccag aatcatcttt ttatcggggt
    22201 cgataaacgg gtaatttgcc gccagttctg aacgcagcgt atcgctgctc atcacctggt
    22261 cacgcaccca taacagtgca tcaataactg tattaccagt aatgaagatt cggctatccg
    22321 caacgttttc acgcagcaag ttttgccggg aagtttcggt tggagagaag tgatacatcg
    22381 ccagatgccc ggtcaatgta cggttagcct cttccggcca cggcgaataa agatcgcccg
    22441 tgcgcagacc agcctcaacg tgaccaacag gaatacgctg ataaaacgcc gccaggctgg
    22501 ttgccagcgt cgtcgttgtg tcgccgtgaa ccagcacgac gtctggtttg aactcggcaa
    22561 gaataggttt tagcccttcc agaatccgac aggttatctc tgtcaggccc tgtcctggct
    22621 gcattatgtt gagatcgtag tcaggtacaa tggaaaagag tttcagcacc tgatcgagca
    22681 tctcccgatg ctgcgcagtg acgcaaactt tagcctcaaa aaaaggatct tttgccaacg
    22741 catgcaccaa cggcgccatc ttgatggctt ccgggcgcgt accaaataca gtcagtactt
    22801 tcacatcgat tctcttcgaa taagcggcga gcgcctttgc gctcaccgca gcagtgttgc
    22861 tatttcgagc aacggcgggt taatgcgaca ccagccccga tcagcccccc gacaatgccc
    22921 cacataatca tcaggaaggc acgacgtggg ctatcgcgtt ttaccggttc ttccggcgta
    22981 cgcaaatagc gataggtctg aaaacgcgga tccagggttg gaccaacatt cagggtgttt
    23041 aacatggccc gattctgatc atagtcgaga tcaaaggccg gaccgacggc ctgtaaattt
    23101 tccagtcgag cctggagcat tggacgccca agcaggaaca tttctgaatc aggtaattcc
    23161 tcggcaggca catctgtcgc actgcgcgaa atattatgct gctcagcaat tttcagcgcc
    23221 tgttcaatac tgttcatccg gcggtcgtag atggctttcg ccacctcttc ctgacgcttc
    23281 acctgtgctt tcatctggat ggtacgcgcc gcccatgcgc ctttcagctc atcattcaga
    23341 tggctggctg cacgctggct ggcaaaagca acatactgac gtaacaggtt attagcgtca
    23401 ggcgcggttt cagcaataag cttcacgctg tcattgaccg cgcgggtaaa gtctccgggg
    23461 ataaactgga tgttgttaat catttcatcc agcaacgccg catcggcttt gctgttgccc
    23521 accatccgct gtttgtaata gtcggtttgc agccagaact ctctgcgggt atcccacgag
    23581 gccagttgca taacaaactc tttataggct tcgtccatga ccgatggttg gtcggcagaa
    23641 gccatgtttg aacggacatc caggttacgc aaaaattgct gctgcgagta atatcccccc
    23701 agcatattca ccgttggacg atcggtaatc gccgtcgagc tccactcctg acgagcaaaa
    23761 aaagtatacg ccagcgcgat taacgcaaat gccagcccca tgccaataat ccatagcttc
    23821 ccagcccaca aggtacgaaa caacccacga atatccagtt cattttcagc gtcttcggcc
    23881 ggtttcccag gcattggttg tgtcatcaca tcctcattta tttggttaaa ttggggctgc
    23941 caccacgatt tctacgcagt ctgcgtttta cgcgcttaat aaagcgagca actttccagg
    24001 cacgcttaat gcaatatcca tagaggaaga atgctagcaa aaagagcacc agcatgaccc
    24061 actccgggac aaaatgagaa tattctgcca gcacgccaat ggaagcgagc agtgccgcag
    24121 caagggtaat cagcacaaac gcctgacggg aagtaaaccc ggcacgcatg atcaaatggt
    24181 gaatatgctg acggtcagga gagaatgggc tcatgccttt acgcaggcga cggtacataa
    24241 tcgccaccat atccattagc ggaatggcga ttatccacaa agcggtaacc gggctgatgg
    24301 gatgggtttt gccctgggtc gtttcgagca ggatccagat aacggtaaaa ccaatcagcg
    24361 tactgcccgc atcacccata aagactttgt agcggcgacc caggatacca aggttaagca
    24421 tgatgtatgg caggatggcg gcgatcatcg caaagcacca gattgcgagg ctggtttgcc
    24481 cgtcgaacca caaaatcata ccgattgctg caaacgagac gcaggacaac ccgcccagca
    24541 agccatcaat gccatcaacc atgttgaacg cattaatggc cgcccagacg gcaaatagcg
    24601 tcaggaagta accaaacggt ccgagcacca tctcccagga gccaaagata taacccaggc
    24661 tactgagata aagtttgccg aacaccatca taacaatgcc aacagcggcc tgtatggtgg
    24721 cacggatttt tacgctgata tcaaaacggt catccagcgc gccaatgaaa acaagcacac
    24781 cggcacaagc gagatagaga gatgcatgcg gaatatagta atcgacaatt ccgaacgtga
    24841 agcaaatccc tgcgtaaacc gaaatccccc caacgagagg tatcaatccc tggtgacgtt
    24901 tgcggaagtt tggtttatcc actaaaccga ctttttttgc caccttacgg gcaaaaaaca
    24961 gaaacagtgt cgtgaataaa aaaatactga tgagatcagt actcactgtc agtaaattca
    25021 caatgcatgc tctcagagaa aattattagc agaagtataa ccacgaagac ctttattcag
    25081 aagggaaagt ccgttccgaa cccaccagtc ctgtgcaaaa attaaccatt tgatatgcaa
    25141 attgcataac acgccagcaa attccaggaa ttttcctaac atcgccagcg cggcattaag
    25201 attacagata taaaagcaaa acgccacgta aacacgtggc gtttttggca taagacaaat
    25261 ttatgagcgt ttcatcattt cgaagaaatc gtcattggtc ttggtcatgg ccagtttatt
    25321 aatgaggaat tccattgcat cgatttcgcc catcgggtga atgattttgc gcaggatcca
    25381 cattttctgc agttcttcct gagtagtgag cagttcttct ttacgggtac cggaacggtt
    25441 gtagtcgata gccgggaaga cgcgtttttc agcgatctta cgagagaggt gcagttccat
    25501 gttgcctgta cctttaaact cttcgtagat aacttcgtcc attttagaac cggtatcgat
    25561 aagcgccgtc gcgataatag tcaggctgcc gccctcttcc acgttacgcg ccgcaccaaa
    25621 gaagcgtttc ggacgatgca gggcgttggc atccacacca ccggtcaaca ctttacctga
    25681 cgccggaaca acggtgttgt aagcgcgcgc cagacgagtg atggagtcga gcagaatgat
    25741 aacgtctttc ttgtgctcaa ccaggcgttt ggccttctcg atcaccattt ccgcaacctg
    25801 aacgtggcga gatgcgggtt cgtcaaaggt agaagcaaca acttcacctt ttaccagacg
    25861 ctgcatctcg gttacttctt ccggacgttc gtcgatcagc agaaccatca gcacacaatc
    25921 cgggtggttg taagcaatgc tctgggcaat gttctgcagc agcatggttt taccggcttt
    25981 cggcggtgcc acaatcagac cacgctgacc acgaccgata ggtgatgcca gatccagtac
    26041 gcgagcagtt aaatcttcag tagaaccgtt accacgttcc atacgcagac gagaatttgc
    26101 gtgcagcggg gttaagttct caaagaggat tttgttgcgg gcgttttcag gtttgtcgaa
    26161 gttaacttcg ttaactttca gcagcgcaaa atagcgttca ccttctttcg gcgggcgaat
    26221 cttaccagag atggtatcac cagtgcggag gttgaaacgg cggatttggc taggggaaac
    26281 gtagatgtca tcaggaccgg cgaggtagga gctgtctgcg gaacggagga aaccaaatcc
    26341 atcctgcaat atctccagta cgccatcacc aaagatatct tcgccactct ttgcgtgctg
    26401 cttcaggatg gcaaaaataa tgtcctgctt acgcatgcga gccaggtttt ccagccccat
    26461 attttcgccg agagtgatca gctcagaaac cggcgtattc tttaattcgg taagattcat
    26521 agtggtgtga gttcttaaac ttggggtaaa tctcgaactt aatgttgtga atggtatggc
    26581 aggatcatcc atgcctgttt acggtcatcg actcatgtct tttcgctgcc tggtcacagg
    26641 agagtacgca gaactgaaac gacaagacgg attgagtgac gagccagaaa tctgttcact
    26701 tcgcatttaa gataaaacgg gaagcgttgg aaattacaag attcaaactt aataaggtat
    26761 gtttaatacg aagtcaacac taagttagca tgactcacgc cgggcgtcca gtttttagcg
    26821 acggggcacc cgaacatgaa attcccttac gccaggttag cgtcgaggaa ctctttcaac
    26881 tgacctttag acagtgcgcc cactttggtt gccgccactt caccgttttt gaacagcagc
    26941 agagtcggga taccacggat gccatatttc ggcgcagtgc cagggttttg atcgatgttc
    27001 agttttgcaa cggtcagttt gccctgatat tcgtcagcga tttcatccag aatcggggcg
    27061 atcattttgc acggaccgca ccactctgcc cagaaatcga cgaggatcgc cccgtccgct
    27121 ttgagtacat ccgtgtcaaa actgtcgtca gtcaggtgaa taattttatc gctcatatat
    27181 aactccacag gaataagcct ggcgtgttgg tttcgttgtt ggtgtaacat taaccaacta
    27241 aaggttgact ttatttcacc ggatacgctt tcgtaaagca atagtaagct gatattctac
    27301 cacactatga gcaaaacaca tttaacagaa cagaagtttt ccgacttcgc cctgcatccg
    27361 aaggttgtag aagcccttga aaaaaaaggg tttcataact gtacgcccat tcaggcactg
    27421 gcccttccgc tgacgctggc gggtcgtgac gtagccgggc aggcgcaaac cggtaccggg
    27481 aaaacgatgg cgtttcttac gtcaacgttt cattatcttc tctctcatcc tgcgattgcc
    27541 gatcgcaagg tgaatcaacc gcgtgcctta attatggcac cgacgcgtga acttgccgtg
    27601 cagatccatg ccgacgcaga accgctggcg gaagctactg gcctgaagct gggtctggct
    27661 tacggtggtg atggctacga caaacagctg aaagtgctgg aaagcggcgt tgacattctg
    27721 attggcacca cgggtcgttt aattgactac gccaagcaga accacattaa cctcggtgcc
    27781 attcaggtag tggttctgga tgaagccgat cgcatgtacg atctgggctt tattaaagat
    27841 atccgctggc tgttccgccg tatgccgcct gcaaaccagc gcctcaacat gctgttctcc
    27901 gccacgcttt cgtaccgggt acgtgaactg gcgttcgagc agatgaacaa tgccgaatat
    27961 attgaagtgg aaccggaaca gaaaacgggc caccgtatta aagaagagct tttctaccct
    28021 tctaacgaag aaaaaatgcg tttgctgcaa acgctgatcg aagaagagtg gccagacaga
    28081 gcgattattt tcgccaatac caaacaccgt tgtgaagaga tctggggcca cctggcggca
    28141 gatggtcatc gtgtcggttt gttgacaggc gatgtcgcgc agaaaaaacg tctgcgtatt
    28201 cttgatgaat ttacccgtgg cgatctggat attctggttg ccaccgacgt tgccgcgcgt
    28261 ggtttgcata ttccggcagt gacgcacgtc tttaactacg atttacccga tgactgtgaa
    28321 gattacgttc accgtattgg tcgtacaggt cgcgcaggcg caagcggtca ctctatcagc
    28381 ctggcgtgtg aagagtatgc attgaatttg cctgctattg aaacctatat tggtcactca
    28441 attccggtaa gcaaatacaa tccggacgca ttgatgaccg atctgccaaa accgctgcgc
    28501 ctcacgcgcc cacgtacagg caatggtccg cgtcgtactg gcgctccgcg taatcgtcgt
    28561 cgttcaggtt aaataaaatg tcatactcaa aatcattcaa actgtatcaa gatggcaaga
    28621 gaatgaatcc cgatgagcat actgtagtaa gtgattcagg tgaaagaatg cagccaacac
    28681 agagacagat tgaaggatga agagtatggg ttccacctcg tcgctgtatg cagccattga
    28741 tctcggttcg aatagttttc atatgctggt tgtgcgcgag gtggctggaa gcatccagac
    28801 gctgacgcga ataaaacgca aagtgcgtct ggctgctggc ctgaatagcg aaaatgccct
    28861 gtctaatgaa gcaatggagc gcggttggca atgtctgcgc ctgtttgctg aacgtctgca
    28921 agatatccct ccctcgcaaa ttcgcgttgt cgctacggcg acattacgtc ttgccgtcaa
    28981 tgcgggtgat tttattgcca atgcgcagga aatcctcggt tgtccggtac aggtgatcag
    29041 cggtgaagag gaagcacgtc tgatttatca gggcgttgct cacacaactg gcggtgccga
    29101 tcagcgcctg gtggtggata taggcggtgc cagtactgaa ctggtaaccg gcacgggtgc
    29161 acaaaccacc tcgttgttca gcctgtcgat gggctgcgtc acctggctgg aacgctattt
    29221 tgccgatcgt aatctggggc aggaaaattt tgatgctgca gaaaaagcgg cacgcgaagt
    29281 gttacgtccg gttgccgatg aattacggta tcacggctgg aaagtgtgcg ttggtgcttc
    29341 cggcaccgtg caggcgttac aggaaatcat gatggcacag gggatggatg aacgcattac
    29401 cctggaaaag ttgcagcaat tgaaacagcg agccattcat tgcggtcggc tggaggaact
    29461 ggagattgac gggctgacgc tggaacgtgc gttagtgttc ccgagtggtc tggcgatcct
    29521 gatcgccatt tttaccgaac tgaatattca gtgtatgacc ctggcgggcg gtgcgctgcg
    29581 tgaaggcctg gtctacggca tgttgcatct ggccgtcgag caggatattc gcagccgtac
    29641 gctgcgtaat attcagcgcc gctttatgat cgatattgat caggcgcagc gcgtagccaa
    29701 agttgcggct aacttcttcg atcaggtgga aaaggaatgg catcttgaag caataagccg
    29761 cgatttgctc atcagcgcct gtcagcttca tgaaatcggc ctgagcgttg actttaaaca
    29821 agcgccgcaa cacgctgctt atctggtgcg taatctggat cttcccggtt ttacccccgc
    29881 acagaaaaaa ttgctggcga cgctactgct caaccagact aatccggtcg atctctcatc
    29941 gctgcatcag caaaatgccg taccgccgcg cgtcgcagaa caactctgcc gtttactccg
    30001 cctggccatc atttttgcca gccgtcgccg tgacgatctc gtgccagaga tgacattaca
    30061 ggctaaccat gaactgttga ccttgacgct tccgcaaggt tggctaaccc aacatccgct
    30121 gggtaaagag attattgctc aggaaagcca gtggcagagc tatgtccact ggccgctgga
    30181 agtgcattaa tgagtaagtg ccggatgcga tgctgacgca tcttttccgg ccttgattat
    30241 ttccctcgtt ttgccgccat catcgctttc agattcgcca gatggctttg ccctttctgc
    30301 atccgttctt cggcgctgac cactttgcgc tcctgttccc aaatcagatc atcctgcggc
    30361 agctccagca aaaagcggct cggctccggg cgcaccagct cgccgtactg acggcgttct
    30421 ttacacagcg taaaggtcaa ttccttctgg gcgcgggtaa tgccgacata ggccagccgc
    30481 cgctcctcat cgatattatc ttcatcgatg ctgctctggt gcggcaaaaa cccttcttcc
    30541 ataccgacca tgtagacata aggaaactcc agcccttttg acgcgtgcag agtcatcagt
    30601 tgcacctgat ccagctcttc ttcactctca ccacgctcca tcatgtcgcg caaagtaaag
    30661 cgcgtcacca cctgggtgag cgtcatcggc tcatccagtt cgctgccttc cagcatctcc
    30721 gtcatccagc taaacagttg gttgacgttc ttcatgcgca tttcggcggc tttcgggctg
    30781 ggcgatgttt cgtacagcca ggattcataa tccatgccgt ggatcagatc gcgcaccgcg
    30841 gcaatcggct cccgctccgc cagacgctgg atttctgcca accagtgagt aaagcgggtc
    30901 aatgcttcat aaccacgtcc gctaagcgtc tgactcaggc ccatatcaaa gctggcggta
    30961 aacatgcttt tattgcgcgt catcgcccac tcacccagct ttttcagcgt cgccgggcca
    31021 atctctctct tcggcgtgtt aacgatacgc agaaatgcgc tgtcatcgtc cgggttagtc
    31081 agcacgcgca gataagccag caagtccttg atttcaggac gagagaaaaa cgacgtacca
    31141 ccagaaattt tgtacgggat gcggttttgc atcaggaatt tttcaaacac tcgcgactga
    31201 tggttaccgc gataaagaat agcgtaatct ttgtactgcg ttttattgac gaagtgatgc
    31261 gcaatcagtt caccagtgac gcgctcggct tcatgttctt cgttattcgc gcttaaaact
    31321 ttaagctctg tgccgtaacc cagttcggag aacagacgct tctcaaagac gtgcgggtta
    31381 ttggcgatca ggatgttcgc cgctttcaaa atacgtccgg aagagcggta gttctgctca
    31441 agcttaatca ccttcagcgc cggaaaatcc tgactcagca gcaccaggtt ttgtggacgt
    31501 gcgccgcgcc aggagtagat cgactggtcg tcatcgccca ccacggtaaa gcgcgcgcgg
    31561 ctgcccacca gcagtttcac cagttcatac tggctggtgt tggtgtcctg atactcatcc
    31621 accagcagat agcgaatttt gttctgccag cgctcgcgga cttcttcatt gcgttgcagc
    31681 agcaacgtcg gcaataaaat cagatcatcg aagtcgagaa cattgcacgc tttcaggtgt
    31741 gcatcataca gcccataaca atgggcaaaa atacggtccc gctcgccaat cgcactggct
    31801 gccgcctggg acggtgtttt gagatcgttc ttccagttag agatggtcga aatcaactgt
    31861 tgcaggagaa ctttgtcatc ttcaatcagc ccttcggtca actctttgag caacgccagc
    31921 tgatcggtat cgtcaaacag cgaaaagttc gccttcatcc ccagcgccgc atactcacgc
    31981 ttgatgatat ccagccccag cgtgtggaag gttgagatca tcagcccacg cgcctctttg
    32041 cgacccagcg tctgtccgac acgctctttc atctcgcgcg ccgccttatt ggtaaaggtc
    32101 accgccgcga tatgtcgcgc ctgataaccg caaccgcgga tcagatgggc gattttattg
    32161 gtgatcacac gagttttacc ggaacccgcg cccgccagca ccaggcaggg gccggtaacg
    32221 aattcgacag cttgttgttg gccggggttt agacgcatag gtgtattgct caatcttcga
    32281 acggggggag gattgtagca gaatcgcatt tgaatgtgac cgcgtgtaag ccgctaaccg
    32341 attggctgcg gatgtttaaa tcagagaggg aagaccgaaa ccggagttca tcgtgaatcc
    32401 attagactta gaaaatatcg ggtcgtaacg ttgtgttatt actttttcag agtttactaa
    32461 ggagaggtgt actcgtggcg attaatttca gtcctaaggt tggtgaaata ctggattgca
    32521 actttggtaa ctatcctgtt agccagaatg gacagttcag caccacgtat tacgatggcc
    32581 gcattcctcc tgaaatgata aaaaacaggt tagtt
//
LOCUS       DAJTVK010000036        32532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000036 DAJTVK010000000
VERSION     DAJTVK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32532
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.036"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..94
                     /locus_tag="OO372_001728"
     CDS             <1..94
                     /locus_tag="OO372_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6341248.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
     gene            complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="OO372_001729"
     CDS             complement(136..1212)
                     /gene="glpQ"
                     /locus_tag="OO372_001729"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6341249.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="OO372_001730"
     CDS             complement(1217..2575)
                     /gene="glpT"
                     /locus_tag="OO372_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6341250.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            2848..4476
                     /gene="glpA"
                     /locus_tag="OO372_001731"
     CDS             2848..4476
                     /gene="glpA"
                     /locus_tag="OO372_001731"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6341251.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4466..5725
                     /gene="glpB"
                     /locus_tag="OO372_001732"
     CDS             4466..5725
                     /gene="glpB"
                     /locus_tag="OO372_001732"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6341252.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5722..6912
                     /gene="glpC"
                     /locus_tag="OO372_001733"
     CDS             5722..6912
                     /gene="glpC"
                     /locus_tag="OO372_001733"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6341253.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7105..8007
                     /locus_tag="OO372_001734"
     CDS             7105..8007
                     /locus_tag="OO372_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6341254.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="OO372_001735"
     CDS             complement(8048..8851)
                     /gene="yfaU"
                     /locus_tag="OO372_001735"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR6341255.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(8869..10158)
                     /locus_tag="OO372_001736"
     CDS             complement(8869..10158)
                     /locus_tag="OO372_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6341256.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="OO372_001737"
                     /pseudo
     CDS             complement(10215..11419)
                     /gene="rhmD"
                     /locus_tag="OO372_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(11434..12216)
                     /locus_tag="OO372_001738"
     CDS             complement(11434..12216)
                     /locus_tag="OO372_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR6341257.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12436..13638)
                     /locus_tag="OO372_001739"
     CDS             complement(12436..13638)
                     /locus_tag="OO372_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6341258.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13738..14280)
                     /locus_tag="OO372_001740"
     CDS             complement(13738..14280)
                     /locus_tag="OO372_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341259.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14559..14984
                     /gene="nudI"
                     /locus_tag="OO372_001741"
     CDS             14559..14984
                     /gene="nudI"
                     /locus_tag="OO372_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6341260.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15023..15625)
                     /locus_tag="OO372_001742"
     CDS             complement(15023..15625)
                     /locus_tag="OO372_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6341261.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            15933..17072
                     /gene="arnB"
                     /locus_tag="OO372_001743"
     CDS             15933..17072
                     /gene="arnB"
                     /locus_tag="OO372_001743"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6341262.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNRERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17076..18044
                     /gene="arnC"
                     /locus_tag="OO372_001744"
     CDS             17076..18044
                     /gene="arnC"
                     /locus_tag="OO372_001744"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6341263.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18044..20026
                     /gene="arnA"
                     /locus_tag="OO372_001745"
     CDS             18044..20026
                     /gene="arnA"
                     /locus_tag="OO372_001745"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR6341264.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20023..20913
                     /locus_tag="OO372_001746"
     CDS             20023..20913
                     /locus_tag="OO372_001746"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6341265.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            20913..22565
                     /gene="arnT"
                     /locus_tag="OO372_001747"
     CDS             20913..22565
                     /gene="arnT"
                     /locus_tag="OO372_001747"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6341266.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22562..22897
                     /gene="arnE"
                     /locus_tag="OO372_001748"
     CDS             22562..22897
                     /gene="arnE"
                     /locus_tag="OO372_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6341267.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            22897..23283
                     /gene="arnF"
                     /locus_tag="OO372_001749"
     CDS             22897..23283
                     /gene="arnF"
                     /locus_tag="OO372_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6341268.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="OO372_001750"
     CDS             complement(23277..23543)
                     /gene="pmrD"
                     /locus_tag="OO372_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6341269.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23587..25008)
                     /gene="menE"
                     /locus_tag="OO372_001751"
     CDS             complement(23587..25008)
                     /gene="menE"
                     /locus_tag="OO372_001751"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6341270.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(25005..25967)
                     /gene="menC"
                     /locus_tag="OO372_001752"
     CDS             complement(25005..25967)
                     /gene="menC"
                     /locus_tag="OO372_001752"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6341271.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(25967..26824)
                     /gene="menB"
                     /locus_tag="OO372_001753"
     CDS             complement(25967..26824)
                     /gene="menB"
                     /locus_tag="OO372_001753"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6341272.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(26839..27597)
                     /gene="menH"
                     /locus_tag="OO372_001754"
     CDS             complement(26839..27597)
                     /gene="menH"
                     /locus_tag="OO372_001754"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6341273.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27594..29264)
                     /gene="menD"
                     /locus_tag="OO372_001755"
     CDS             complement(27594..29264)
                     /gene="menD"
                     /locus_tag="OO372_001755"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6341274.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29353..30648)
                     /gene="menF"
                     /locus_tag="OO372_001756"
     CDS             complement(29353..30648)
                     /gene="menF"
                     /locus_tag="OO372_001756"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6341275.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="OO372_001757"
     CDS             complement(30727..31032)
                     /gene="elaB"
                     /locus_tag="OO372_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6341276.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31087..31548)
                     /locus_tag="OO372_001758"
                     /pseudo
     CDS             complement(31087..31548)
                     /locus_tag="OO372_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            31613..32530
                     /gene="rbn"
                     /locus_tag="OO372_001759"
     CDS             31613..32530
                     /gene="rbn"
                     /locus_tag="OO372_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6341277.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7897 a         8803 c         8139 g         7693 t
ORIGIN      
        1 ttcgttgttg gctaaagaaa agccggtaga aggcagtcca ctgtttttac tacaaatgtg
       61 catggaacaa ttagtggcga ttggaaaaat ttgatatgag acaggatggc gcatgaacgc
      121 catcctgcag tagcattact ctttattaag gaattttact gccttatcag ggaaatcagt
      181 aaacagccca tttacacccg ctttgttata cagagcatca tataactgat tcacatcagt
      241 agtgtattca ggcagtttat ccgaccgcac ggtataagga tgcactacca gtttattctg
      301 ctgagcatct tgcaccatgc cagtgagttt gatattaccc ggctgcgatg tttcctcaat
      361 caacatatgg taatccggcc cgataccgtc ggcatattcc gcgacttgct tcatggcacc
      421 tggcttaaac atccagtcat agctgtaatt aacccagctt ccgtccggct gtttctgctg
      481 cgtttcattc cagtcggtat aggcaatcag ctgtaccaga ttgagatcca tgcccatttt
      541 aggttccagc tcattcttaa tacgcttcag ctcatcagca tcaaaacatt gcaaataaac
      601 tttatcgtcc ttaccggtgt aaccatattt cttcagcact tccagcgttt ttgccgcaat
      661 atccttccct tcctgatgat ggaaccacgg cgctttgatt tctggataga taccgatatt
      721 tttcccggta gagtgattta acccctgaac aaattcaatc tcttcttcaa aggtgtgccc
      781 ccggaagtcg gacttaccca ttgggaaacg ccccggataa gtctgtactt ttttaccgtt
      841 ttcaatatcg aaaccttcgg taaatttcag cgacttaatt tcatccagcg tgaaatctat
      901 cgcgtagtaa cgaccgtctt tgcgcgcccg atccgggaaa cgatcggcaa catcagtaac
      961 acgatcgaga taatggtcat gcagaacaac cagatggtcg tctttggtca tcaccaaatc
     1021 ctgttccaga taatccgctc cctgcgcata cgccatcgct tttgctggca gcgtatgctc
     1081 cggcaaatat ccactggcac cgcgatgggc gatgactatt ttttcgttgc tgtccgccgc
     1141 cattgcactg cttcccatga ctatagtgct catcatgatc gccatgctaa ggtttttcag
     1201 cttcaatttc atgccattag cctccgttgc gtttttgcag taattgttca tggcgacgtt
     1261 tttcgccaat catcacaaca atcaacaaga taaccgccag aatgctgccg ccaatcatta
     1321 ccataaagcc gccatcccag ccgaagaagt ccacggtgta gccaacaatc gcgctcgccg
     1381 ccaccgaacc gcccaggtaa ccaaacagcc cggtaaagcc cgctgccgta cctgccgctt
     1441 ttttcggtgc cagttccagc gcatgcagac cgatcagcat cacaggaccg tagatcagga
     1501 agccgataac aatcatacaa atcatatcga cggttgggtt acctgccggg ttcatccagt
     1561 aaacgatagt cgcgatggtc accagtgtca taaagaaaac gccggttgcc ccacggttgc
     1621 cacggaagac tttatccgac atccagccgc acagcagagt gcccggaata cctgcatatt
     1681 cataaaggaa gtaggcccag gaggatttat ccagcgcgaa atgcttaacc tctttcagat
     1741 aagtcggtga ccagtcgagg atgccgtaac gcagcagata aacgaacacg ttggcgatgg
     1801 cgatatacca cagcagtttg ttcggcagta cgtactgcat gaagatttgc ttcgccgtca
     1861 gctcctgttc cgctttttcg ttatagtcgt ccggataatc atttttgtac tcttcgatcg
     1921 gcggcaagcc acaggattgc ggggtatcgc gcatcatcgc aaaggcgaat aatgccacca
     1981 gaatggcgca gaaagcaggc atatagagcg ccgcatgcca gtcattgaac caggccatcc
     2041 ccagcaggaa cagcagcggc ggaataccac caccgacgtt gtgcgcacag ttccacactg
     2101 acacaatgcc gccacgttct ttctgcgacc accagtgcac catagtacga ccacacggcg
     2161 gccaccccat cccctggaac caaccgcaga ggaacaacag tacaaacatc accgcaatgc
     2221 tcgacgtcgc ccatggcaca aagcccataa acaacatcac tgccgccgcc agaatcaaac
     2281 ctgcgggcag gaaaacgcgc ggattcgagc gatccgatac cgaacccatg atgaatttcg
     2341 aaaatccata agcaatcgag atccccgaaa gggcaaaacc taaatcaccg cgtgagaatc
     2401 cctgctcaac cagataaggc atagcaagcg caaagttctt acgaaccaaa tagtaagccg
     2461 catagccaaa gaatatcccc aggaaaattt gccagcgcaa tcgacgataa gtcggatcga
     2521 tctccgcggc aggtaagcgc gctttgtgtg gcgctggttt aaaaatactc aacattgata
     2581 gcctccgtgg cccgtggtct tatttatgat taacagcctg attcagtgag agaacctgcc
     2641 gtttcttgag ttgccgcgat gttaagaaaa cattcataaa ttaaatgtga attgccgcac
     2701 acattattaa ataagattta caaaatgttc aaaatgacgc atgaaatcac gcttcacttt
     2761 cgaatcatga gcgaatatgc gcgaaatcaa acaattcatg tttttactat ggctaaatgg
     2821 taaaaaacga acttcagagg gataacaatg aaaactcgcg actcgcaatc aagtgacgtg
     2881 attatcattg gcggcggcgc aacgggagcc gggattgccc gcgactgtgc cctgcgcggg
     2941 ctgcgcgtga ttttggttga gcgccacgac atcgcaaccg gtgccaccgg gcgtaaccac
     3001 ggcctgctac acagcggtgc gcgctatgcg gtaaccgatg cggaatcggc ccgcgaatgc
     3061 attagtgaaa accagatcct gaaacgcatt gcacgtcact gcgttgaacc aaccaacggc
     3121 ctgtttatta ccctgccgga agatgacctc tccttccagg ccacttttat tcgcgcctgc
     3181 gaagaagcag ggatcagcgc agaagctata gacccgcagc aagcgcgcat tatcgaacct
     3241 gccgttaacc cggcattgat tggcgcggtg aaagttccgg atggcaccgt tgatccattt
     3301 cgtctgaccg cagcaaacat gctggatgcc aaagaacacg gtgccgttat ccttaccgct
     3361 catgaagtca cgggactgat tcgtgaaggc gcgacggtgt gcggtgttcg tgtacgtaac
     3421 catctcaccg gcgaaactca ggcccttcat gcgcctgtcg tggttaatgc cgccgggatc
     3481 tgggggcaac acattgccga atatgccgat ctgcgcattc gcatgttccc ggcgaaagga
     3541 tcgctgctga tcatggatca ccgcattaac cagcatgtga tcaaccgctg ccgtaaacct
     3601 tccgacgccg atattctggt gcctggcgat accatttcgc tgattggcac cacctcttta
     3661 cgtattgatt acaacgagat tgacgataat cgagtgacgg cagaagaggt tgatattctg
     3721 ctgcgtgaag gggaaaaact ggcccccgtg atggcgaaaa cgcgcatttt gcgggcctat
     3781 tctggcgtgc gcccgctggt tgccagcgat gacgacccga gcggacgtaa cgtcagccgt
     3841 ggcatcgtgc tgctcgacca tgctgaacgc gatggtctgg acggatttat caccatcacc
     3901 ggtggcaaac tgatgaccta tcggctgatg gctgaatggg ctaccgacgc cgtgtgccgc
     3961 aaactgggca acacgcgccc ctgtacgacc gccgatctgg cacttcctgg ttcacaggaa
     4021 cccgctgaag ttaccttgcg taaagtcatc tccctgcctg ccccgctgcg cggttctgcg
     4081 gtttatcgtc atggcgatcg cacgcctgcc tggctgagcg aaggtcgtct gcaccgtagc
     4141 ctggtatgtg agtgcgaagc ggtaacagcg ggtgaagtgc agtacgcggt agaaaattta
     4201 aacgttaata gcctgctgga tttacgccgt cgtacccgtg tggggatggg tacctgccag
     4261 ggcgaactct gcgcctgccg cgctgccgga ctgctgcaac gttttaacgt cacgacgtcc
     4321 gcgcaatcta tcgagcaact ttccaccttc cttaacgaac gctggaaagg cgtgcaaccc
     4381 atcgcctggg gagatgcact gcgcgaaagc gaatttaccc gctgggttta tcagggactg
     4441 tgtggtctgg agaaggagca gaaagatgcg ctttgatact gtcattatgg gcggcggcct
     4501 cgccggatta ctctgtggcc tgcaactgca aaaacacggc ctgcgctgtg ccattgtcac
     4561 gcgtggtcaa agcgcgctgc atttctcatc cggttcgctg gatttgctga gccatctgcc
     4621 tgatggtcaa ccggtgacag acattcacag tggactggaa tctttgcgtc agcaggcacc
     4681 agcccatcct tacagtcttc tcgaaccaca acgcgtgctc gatctcgctt gccaggcgca
     4741 ggcattgatc gctgaaagcg gtgcgcaatt gcagggcagc gtggaactgg cccatcagcg
     4801 cgttaccccg ctcggcactt tgcgtgcaac ctggttaagt tcgccagaag ttcccgtctg
     4861 gccgctaccc gcgaagaaga tatgtgtagt ggggataagc gggctgatgg atttccaggc
     4921 gcatcttgcg gcggcttcgt tgcgcgaact ggatctttcg gttgaaaccg cagaaataga
     4981 gctgccggaa ctggatgtcc tgcgcaataa cgccaccgaa tttcgcgcgg tgaatatcgc
     5041 ccgtttcctt gataatgaag aaaactggcc gctgttactt gatgcgctta ttcctgtcgc
     5101 caatacctgc gaaatgatcc tgatgcccgc ctgcttcggc ctggccgatg acaaactgtg
     5161 gcgttggttg aatgaaaaac taccttgttc actgatgctt ttgccaacgc tgccgccttc
     5221 cgtgctgggc attcgtctgc aaaaccagtt acagcgccag tttgtgcgcc agggtggcgt
     5281 gtggatgccg ggcgatgaag tgaaaaaagt gacctgtaaa aatggcgtag tgaatgaaat
     5341 ctggacccgc aatcacgccg atattccgct acgtccacgt ttcgcggttc tcgccagcgg
     5401 cagtttcttt agtggcggac tggtagcgga acgtaacggc attcgcgagc cgattctcgg
     5461 ccttgatgtg ctacaaaccg ccacgcgggg tgaatggtat aagggcgatt tttttgcgcc
     5521 gcaaccgtgg cagcagttcg gtgtaaccac tgatgagacg ctacgcccgt cgcaggcagg
     5581 gcaaaccatt gagaacctgt ttgccatcgg ttcggtgctg ggcggatttg atcccatcgc
     5641 ccaaggatgc ggcggcggtg tttgtgccgt cagtgcttta catgccgctc aacagattgc
     5701 ccaacgcgca ggaggccaac aatgaacgac accagcttcg aaaactgcat taagtgcacc
     5761 gtctgcacca ccgcctgccc ggtgagccgg gtgaatcccg gttatccagg gccaaaacaa
     5821 gccgggccgg atggcgagcg tctgcgtttg aaagatggcg cactgtatga cgaggcgctg
     5881 aaatattgca tcaactgcaa acgttgtgaa gtcgcctgcc cgtccgatgt gaagattggc
     5941 gatattatcc agcgcgcgcg ggcaaaatat gacaccacgc gcccgtcgct gcgtaatttt
     6001 gtgttgagtc ataccgacct gatgggtagc gtttccacgc cgttcgcacc aatcgtcaac
     6061 accgctacct cgctgaaacc ggtgcggcag ctgcttgatg cggcgttaaa aatcgatcat
     6121 cgtcgcacgc taccgaaata ctccttcggc acgttccgtc gctggtatcg cagcatagcg
     6181 gctcagcaag cacaatataa agaccaggtc gctttctttc acggctgctt cgttaactac
     6241 aaccatccgc agttaggtaa agatttaatt aaagtgctca acgcaatggg tatcggtgta
     6301 caactgctca gcaaagaaaa atgctgcggc gtaccgctaa tcgccaacgg ctttaccgat
     6361 aaagcacgca aacaggcaat tacgaatgta gagtcgatcc gcgaagctgt gggagtaaaa
     6421 ggcattccgg tgattgccac ctcctcaacc tgtacatttg ccctgcgcga cgaatacccg
     6481 gaagtgctga atgtcgacaa caaaggcttg cgcgatcata tcgaactggc aacccgctgg
     6541 ctgtggcgca agctggacga aggcaaaacg ttaccgctga aaccgctgcc gctgaaagtg
     6601 gtttatcaca ctccgtgcca tatggaaaaa atgggctgga cgctctacac cctggagctg
     6661 ttgcgtaaaa tcccggggct tgagttaacg gtgctggatt cccagtgctg cggtattgcg
     6721 ggtacttacg gtttcaaaaa agagaactac cccacctcac aagccatcgg cgcaccactg
     6781 ttccgccaga tagaagaaag cggcgcagat ctggtggtca ccgactgcga aacctgtaaa
     6841 tggcagattg agatgtccac aagtcttcgc tgcgaacatc cgattacgct actggcccag
     6901 gcgctggctt aaactccttt ctgatgcccg gtaagcatgt ggttaccggg catttttgcg
     6961 tacacgattc cgtgcccaat gtatgcgttg caacgcagtg aaaattcctc tgaaaacgtc
     7021 tcgcaaaggc tgaaactggc agatgtcaaa ggcctgggat aaccgtaatg tcgcgtcatc
     7081 ataaatatca ggtgacggac aaccatgacc gaatcaacaa cctcctcccc gcatgatgcg
     7141 gtatttaaaa cctttatgtt cacacccgaa accgcacggg attttctcga aatacatttg
     7201 ccagaaccac tgcgcaagct ttgcaacctg caaaccttac gcctggaacc cactagtttt
     7261 attgaaaaaa gtttacgcgc ttactactcg gatgttttgt ggtccgtgga aaccagcgac
     7321 ggtgacggct atatctactg cgtgattgaa catcaaagct ctgcagaaaa gaatatggct
     7381 tttcggctaa tgcgctatgc cactgccgcc atgcagcgtc acctggataa aggctatgac
     7441 agagttccgc tggtggtgcc attgctgttt tatcatggcg aaacatcgcc ctacccgtac
     7501 tcacttaact ggctggatga gtttgacgat ccgcaacttg cccggcagtt gtacaccgaa
     7561 gcttttccgt tggtggatat caccatcgta cctgacgatg agatcatgca acatcggcgt
     7621 atagctctgc ttgaactgat tcaaaagcat attcgcgacc gcgatttaat cggtatggtc
     7681 gacaggatca ccacgctttt ggttaaaggc ttcactaatg acagccagct acaaacactg
     7741 tttaattatc tgctgcaatg cggcgatacc tcccgtttca cccgttttat tgaggagatt
     7801 tccgaacgtt caccactaca aaaggagaga ttgatgacta ttgctgagcg gctacggcag
     7861 gaaggacacc aaattggctg gcagaaaggt atgcatgaac aagccattaa aattgctttg
     7921 cgcatgctgg aacagggcat tgatcgtgac caggtgctcg cggccaccca gctaagcgaa
     7981 gccgatctgg cagcgaataa ccactaatta acacaggccc acagccgatc cccatgggcc
     8041 tttgatatca ataactacct tttatgcgtg gcccattttt gcctgattta aacatcgcca
     8101 gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac gccaacagcg acaaagttcg
     8161 ctccccacgc caggcattgc tgcgccatat caggagccac agccagaaaa ccagccgctt
     8221 tacccgcagc acggatccgc cgaatgctgg tttcaataat tcgctgcact tccgggtgcc
     8281 cggcgttatc cgggtagccc aacgacgcag aaagatccgc tggtccaata aacacgccat
     8341 caatcccttc gacgtcgagg atctcgtcca ggttatccag tgccgtttta ctttccacct
     8401 gcactaacag acaaagcgaa tcgttgactt gcgccatgta attctcaatg cgtccccagc
     8461 gcgcagcccg tgccacactg gccccaacac cacgctcacc gtagggagga tagcgcgtgg
     8521 cagacgccac ctgacgagcc tgatcggcag tatcgaccat cgggatcaat agagtttgcg
     8581 cgccaatatc cagaacttgt ttaatcagcg atttactgcc ttccaccgga cggatcacgg
     8641 gttggctggc atagggcgct accgcctgta gctggtgata aagatcctga atggtgtttg
     8701 gcgcgtgctc cccgtcaatc agcaaccagt cataaccgga agtagcggca atttctgcca
     8761 tataagaagt agtcgagctt aaccacagac caatttgcac ttcgcccttg cgtaaacgtt
     8821 ctttaaaggg attggttaat aatgcgttca tcgtgttcct taacctattt aatgatgtgc
     8881 cacgtcggtc tgcgcaacag tacgatttac acgcagagtg aaaataatca gcgaaccgat
     8941 gaccgccacc gctgccagtg tcagtaatcc tgccgcatcg ctggcaaata acgtttctgc
     9001 tttcacgcgc aggatcgggg caataaaccc gcccactgca ccaaacaggt ttacaaaacc
     9061 aatccccgca gccagcgccg taccggaaag cagttgtgtc ggcatcgtcc agaacaccgg
     9121 ctgcacggca ataaacccga tagctgcaac acacagcgct acgatcgcca ttactggaga
     9181 aagcagcccg gacagaccaa taccaatgcc cgccgccagt aatgtcagcg ctgcgacatt
     9241 acgccgttcg ccggttttat cggaatagcg cggaataagc caggtcccaa acaaggccgc
     9301 aacccacgga atggcggtga ccaccgacgc tgtaaagccc acttttgttc ccagcaatgc
     9361 cgcaacctgg gtcggcagga agaaaattaa tccgtaaacc gccacctgaa tggtcaggta
     9421 gataatcgct agttgccaga ctcggccatt acgcagcgca tcgctcagcc gagaagtcac
     9481 tttctgttgt tcttcacttg ccagttgatt gataagcaac gttttttctt gtttactcag
     9541 aaaacgtgcc tgctccggtg tgtcatcaag ccaaaagaat gtgaataccc cagcaccgac
     9601 tgccaacaat ccttcaatca caaacatcca gaaccagccg ggatgcccca taaatccatg
     9661 catctccaac agcgcgccgg aaagcggtga tcccagtgtt aacgccagcg gtgcgcccat
     9721 atagaacagc cccataatgc tggcgcgatt acgctgcgga aaccattgcg aggtgagata
     9781 aatcatacca gggaaaaatc cggcctccgc agcaccaagc agagtgcgaa ctatcagaaa
     9841 tttcgcttca gtatcggccc atgccatggc tgccgaaaga aatccccaca gcagtgttgt
     9901 cgtaccaatc caggttctgg cccccagttt gcgcatcaaa agattcgccg gaacacccag
     9961 aaacgcatat accacaaaga aaatgcctgc tcccagcgca taagcttcat tactcaaccc
    10021 ggtatcaatc tggtaggtct gtttggcaaa accgatattc gaacggtcga gaaacgccag
    10081 cacatacagc gccaacataa acggaattaa acgcacacgg tttttcttca ccacggcgtc
    10141 aagcaaagcg gtgctcataa aaagctcctt agaatatgtg ggcgttaccg ttgtcacagc
    10201 aacgcccgga gtgattagtg gctgtagggg cgtttcaaat tgcagtcacg attgagttcg
    10261 acgccaaaac cgggtttatc aagcactgat ttatgaatac gaccattcac cggaaccggc
    10321 tcattgagca gaatcgggtc aaactgcgga cgcatcgttg aacaatccgg gctggtcatc
    10381 aggaattcgc tgaatggcgt attggtgaag gtgatcaccg catggtgaga gtaaacagac
    10441 gaaccgtgcg gcaccaccag ttgccccctg gatttggcga ttgcggcaat ttccaccagc
    10501 gtggttaaac caccgcacca gccaacatcc ggctgcataa tgtcgatacc ggtttctgaa
    10561 agcgtacgaa aagattgcag tgtgccgtgg tgctcaccgc tggtgaccat cattccgact
    10621 ggcgcgttgc gtttcagttc gcgataacct tcatactgct gtggcggcag gcactcttcg
    10681 atccatttca ggttataggg cgcgcaagcg tgggccagtt tggtggcata gttcacgtcc
    10741 tgactcatcc agcagtcgag cattaaccag aaatcctcac cgcatttttc acgcatatcc
    10801 gcgaccataa cggcatcttt gcggatcccc gcatcgccat catgtggccc ccagtgcgtc
    10861 ggcattttgc caccgataaa gcccatctct tttgccagat ccggacgcgc gcctgtggcg
    10921 tagaactgaa tctcatcacg aactgcgccg cctaaaagtt tataaaccgg aagcccgacc
    10981 actttgccga acagatccca cagagccaga tcgacacagg aaatcgtgtt catcaccagg
    11041 ccaccagagc cggagtaata cagggtggca ttgagcattt gatcatggat cagtttgata
    11101 tcactgacac atttaccctc aatgaaacgg ttaagatgtt tttcgacaat aaaacagccc
    11161 atttcaccgg ctgtcgaaac agcgaatccg gtctggccgt tttctgcttc gacttcaaca
    11221 atcaacgtgc caagaacgtt aatgccaaat gactggcgtg actgctcgta atcgcggtat
    11281 ttactcatcg gggtggcaat atgatcgtca atccagtgat tcgccccctg gtcgtgataa
    11341 tcaccgcgcc agcacctttt tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt
    11401 tgatttttgg tagggtcatg atgttctcca ttgttatgag gcctgtaagt caaagggact
    11461 tttccatccc aacagacgtg aaatatccct ggcgcaggca atggccttgc ccgccagata
    11521 atcacggtat tcttcattga tttgtaagcg ggtaccgacc accgagatcg cagcggtaag
    11581 ctcgttattg gcgttaaaca ccggcgcagc gacacaacgg acatcggggt aatcttcgcc
    11641 gttgtcatag ctccagccct gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg
    11701 atgagtaatc gttgtgggtg tcgcctgctc ccagaccagc ccttcgataa tactttgctg
    11761 taccgctgca ggttgccagg caagcaggca tttacctatt ccggagcgat aaagcgaaag
    11821 gctttttcct tcatgggaac gcacgctgat agtggctgat gactccactt ttaaaatgta
    11881 ataagcgctg ccgttatcaa taatccccag gtgacataac agcccggtgg tatccatcag
    11941 ttgcgtcaaa cgcggtcgag ccagttcccg gagatccatc ttgcttaacg catgaccaga
    12001 aagctccacc agcctggtcc acaaacaaaa attttcctgg tgatcaaggc taagaaaacg
    12061 ctgacgcctg agttcattaa gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc
    12121 aatgatcgtc gcagcactac aggggccaat gcgggcaatc aaattgagaa tatcaatagc
    12181 ccgtgtcagg gcgggaactt tgcttgattc caacatactg gactccagtc ttagatactg
    12241 gaatcagtct cgttgctcag ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg
    12301 tccagcggac tggagaaatt acgcacaaat ttgtgaatca gtgcacggat tttttcttat
    12361 tgagtacaac aggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    12421 cacagagtgg cggaatcaga cagataaggt catggactca acaacctcaa tccagccatg
    12481 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    12541 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    12601 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    12661 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    12721 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    12781 agaaagttgc aaagccaata aaccaccggt gaattgctcg ctcaacgtca ggctgaactg
    12841 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    12901 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    12961 tgccggtccg gtgagtttca gttcgatgat cggcattgag gagcgatagc ccattgttac
    13021 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    13081 ggtagttaaa cgcagacaaa ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg
    13141 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    13201 caggcaacga ttaagccgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    13261 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    13321 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca ggccttcacc
    13381 ttttgctgtc gcagcggcga gtgcacttaa atcatcactg gtcggtccca gcccgccgtt
    13441 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    13501 gttatccccc accgtattgc ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc
    13561 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggataa
    13621 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    13681 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    13741 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc
    13801 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggat gcataatggt
    13861 gtaacgcgcg ccagcattcg cttcctcata acttttaata ccgctggaga gtgaatccgg
    13921 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    13981 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    14041 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgccagcgac
    14101 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaac gccaggcccg tcgattcagt
    14161 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tgaccgctga tgctcattgc
    14221 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    14281 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    14341 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    14401 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    14461 aagtgcagtt atcatcgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    14521 attctcatgg tttcttttta tttataagga atacccctgt gcgacaacgg actattgtat
    14581 gccctttgat tcaaaatgat ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg
    14641 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    14701 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    14761 cgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    14821 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    14881 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    14941 atgttgccac ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    15001 cactggctgt cagattgaaa atttagtggt taacgaattc cccatccaga taaactttgc
    15061 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    15121 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    15181 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    15241 gtttatctac cgtcagtttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    15301 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    15361 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    15421 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    15481 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    15541 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    15601 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    15661 ttaccgtgaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    15721 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    15781 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    15841 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    15901 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    15961 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca
    16021 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    16081 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    16141 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    16201 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    16261 cgcctgaagc gatcgagtca gccattacgc cacgcactaa agccatcatt ccggtgcatt
    16321 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    16381 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    16441 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    16501 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    16561 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    16621 taacaccggg ctataagtac aatctgaccg atattaacgc cgcgattgcc ctgacacagt
    16681 tagcaaaatt agagcacctc aacacccatc ggcgcgaaat tgcccagcaa tatcagcaag
    16741 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    16801 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    16861 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    16921 aaaaatatta tcgcgagcgt tttcccacgt tgtcgttacc gaataccgaa tggaatagag
    16981 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    17041 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    17101 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    17161 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    17221 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    17281 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    17341 gtcacgttac tggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    17401 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    17461 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac aggcttattc
    17521 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    17581 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    17641 atatcttcgc ccgccgtgcc attgaaattc cagtacatca tgccgagcgt gagtttggtg
    17701 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    17761 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    17821 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    17881 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    17941 gattgctcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    18001 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    18061 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    18121 tagcgccatt tttacccata ccgataatcc cgctgaaaaa gccttttatg gttcggtggc
    18181 tcgtctggcg gcggaaagag gcattccagt ttatgcgccg gataacgtta atcatccgct
    18241 gtgggtggaa cgcattgccc aactctcacc agaggtgatt ttctcttttt attatcgcca
    18301 tcttatttgc gacgagattt tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc
    18361 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac
    18421 ggaaacgggc gttacattgc accgaatggt aaaacgtgcc gatgctgggg ccattgtggc
    18481 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    18541 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    18601 ggaaattgcc cagcgcgaaa acgaagccac ctgttttggt cacagaacgc cagatgacag
    18661 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    18721 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    18781 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    18841 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    18901 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    18961 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    19021 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    19081 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgtcga
    19141 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    19201 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    19261 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    19321 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    19381 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    19441 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    19501 cctcttccgc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    19561 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    19621 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    19681 gttgtaccgc attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    19741 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    19801 aaaacatccg ctgcgccatc acttcccacc gtttgcgggc tttcgtgttg tcgaaagtag
    19861 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    19921 ccgccgctgc ctggactggg aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    19981 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtcggctta
    20041 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc
    20101 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    20161 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    20221 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    20281 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    20341 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    20401 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    20461 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    20521 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccactgct cgaatcagga
    20581 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    20641 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    20701 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    20761 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    20821 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    20881 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    20941 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    21001 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    21061 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    21121 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    21181 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    21241 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    21301 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    21361 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    21421 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    21481 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    21541 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    21601 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    21661 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    21721 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    21781 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    21841 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    21901 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    21961 tcgcttttgg cttcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    22021 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    22081 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtcttttg
    22141 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    22201 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    22261 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    22321 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    22381 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    22441 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    22501 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    22561 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    22621 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    22681 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    22741 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    22801 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    22861 tattggcggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    22921 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    22981 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    23041 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    23101 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    23161 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    23221 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    23281 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    23341 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    23401 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    23461 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    23521 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    23581 tacagattaa cagataaacc tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa
    23641 actaatgatt tcgtaatcct ggcgttgcac ccactctttt aacgcctgac gtgaaatttt
    23701 aataccgccg tttttcagct ccggcggtaa agttagccag cgcaccggtt gttgaaaacg
    23761 agccagctta tctttcaccc attcgccaag atcaacggtt tgctggtcat actccaccac
    23821 cgctaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    23881 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct ctccgccact
    23941 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    24001 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    24061 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    24121 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    24181 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    24241 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    24301 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    24361 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    24421 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    24481 atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc gcactggcaa gatgggcctg
    24541 ataagtatgg acagcggctt tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca
    24601 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    24661 cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    24721 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    24781 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    24841 catcacgccg ctgccctcaa ccacaccctg cgccgcaaag cccgtagcta actcatcgac
    24901 gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta agacgtaagg cgatggcttc
    24961 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caactgctcc
    25021 agtacatcag cgtccactaa cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc
    25081 tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg gcgttaacca ggcagcaatc
    25141 cgcgccagtt gcgttagacc taagctcgac tcaatggaag aactgatcac cgccgtcagc
    25201 cccagcgcat gcgccgcctg tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg
    25261 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    25321 aggctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    25381 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    25441 ttggcaaact gctgaccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    25501 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    25561 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    25621 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    25681 tctgccagct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    25741 tcgccatcgc ctgccagcca gttatttacc caagcaagca gcacaatttg cgcctcttcc
    25801 caggtttcct gactgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgcct
    25861 tcacgcaggc aaacatacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc
    25921 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    25981 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    26041 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcaga cccgcctgac
    26101 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    26161 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    26221 tgacaaggcc catatccagc gcctgttttg catcgtactg acggcacagg aaccagattt
    26281 cacgcgcttt tttctgtccg acgatgcgag ccatgtagga agcgccccag ccgccgtcga
    26341 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgccgcg atagtcaggt
    26401 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    26461 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcagg tgatgtacgc
    26521 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    26581 acgctttatc gcctgcgcca gtcagaatga tcacgccgac gttgtcgtca taacgcgcat
    26641 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    26701 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcaa
    26761 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    26821 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga taacgccagc
    26881 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    26941 ttccgccgcc aaggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    27001 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    27061 catggcggca agggttgcgc cattattgtt actgcgcagc gccaccagct cccggcgttg
    27121 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    27181 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg
    27241 ttcagcattt tgcaacccag gatgcccgcc ttcgacaaca accccacaaa gccccggcat
    27301 cccctggcaa gccgccatca tcgccacccg tcccccaagc gagtacccca ccaaccagaa
    27361 gttaaggatg ttgtaactaa ccagggtttt acgcagtaag tcggtgacat catcaaatcc
    27421 atcgacgcta atcgccgccg aaccaccgtg acctgggaga taaacataca accgtgagta
    27481 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    27541 aaacaccagc cagggtaaac ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa
    27601 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    27661 atttcaatta ccgtggtggt tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc
    27721 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    27781 acgttttgcg gcatcagata gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag
    27841 aaaatttgcc cgccgttgtt gttcaccaca attaatatca gcggcgcgga aacctgacgc
    27901 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    27961 ggtttgccgc ttgcccgctg aacgccggcg gcagtcgaga gcagcccgtc gataccgctg
    28021 gccccacggt tgctgtacac cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga
    28081 cgtaccacca ggctgttacc gacaaacaat tgcccctgtt caggcagata gtcgctgatg
    28141 cgatgcgcca gttgcgcttc gccaaacgca tcgcgacggg caataaccgc ctgcattgcc
    28201 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    28261 tccagccagt cggcaatatt ggcaattaag cggcgtccac gatggtgcgc cggatcaagc
    28321 cgcccttcga tgtcatccac aatccagtac tcttcgggtt cacagcttgc ctgccattgc
    28381 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgttgcagt
    28441 tcgctggtcg ctttggcatt gcctaaccag agatcggcac acggcagcgg ctgtccggtt
    28501 tgcgacagca cgtcgccaat cagcggccag ccaagagttt gcgcccacag tgcaactttt
    28561 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgtttttg tcgccagaag
    28621 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    28681 tcctgccacc agtcgcccag ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca
    28741 tacagcggtt cggcaaacgg gcagttgata tggactccac cagaatgaag cgtaccgaga
    28801 gcatggtcga tggtagaaac cagccaacgt gcggggatat cctgggtcgg gcgcggcaat
    28861 gaaatactgt gcgtggggtg agaggcgaac attcccggct gacgaatcgc ctgattcgcg
    28921 ccgcagtcaa tcagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    28981 aacccagctt caatcagtgc cggatagaga tttgctaccg ccgtaccgga ggtcacaatc
    29041 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    29101 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    29161 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    29221 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    29281 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    29341 tgcgactcat tactattcca tctgtaataa agtacgcagc cctgccgctt tgttgtcgat
    29401 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catataatcg
    29461 cacgacattg ccgctaattt ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa
    29521 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    29581 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    29641 taaacagatc acattatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    29701 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    29761 taaccgttgg cagatatctt ccaccaccag catattctcg cgctggtttt tatcatccgc
    29821 catcagccac tcacctaact gctgcgcctg cttatcatca gggtgatttg ctaccgttcc
    29881 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    29941 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    30001 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    30061 tgcccgagcg agcaccactt tgtcgagctc accttcggcg atggttttcg ttgccagttc
    30121 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    30181 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg ccttaatcgc
    30241 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    30301 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    30361 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    30421 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    30481 ttgccagtag aattgcgggt atgtttgctg actggccagc cagctcaagg catcaaaagc
    30541 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    30601 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    30661 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    30721 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccaacggccg cgcccacacc
    30781 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaactg cctgcttcgc
    30841 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    30901 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    30961 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    31021 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    31081 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    31141 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    31201 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    31261 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgaccaat
    31321 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatatgcca ccagttcatc
    31381 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    31441 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    31501 ctgggaaacc gataattcag agtgatgcag atcttaccat tcaatcataa tgctctcctt
    31561 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    31621 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    31681 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    31741 ccagcatcag ctactgcata ccgcctttaa ccctgggaaa ctggacaaga tttttatcag
    31801 tcaccttcat ggcgatcatc tctttggttt acccggcttg ctgtgcagtc gttctatgtc
    31861 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    31921 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    31981 tggcgaaatt ctcgatgatg gcctgcgcaa agtgaccgct tatccgatgg aacacccact
    32041 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    32101 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    32161 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    32221 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    32281 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    32341 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    32401 cggcaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcaacacct
    32461 gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt
    32521 taacgtttaa ct
//
LOCUS       DAJTVK010000037        31796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000037 DAJTVK010000000
VERSION     DAJTVK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.037"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="OO372_001760"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="OO372_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6341278.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="OO372_001761"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="OO372_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6341279.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="OO372_001762"
     CDS             1894..2751
                     /locus_tag="OO372_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6341280.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO372_001763"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="OO372_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO372_001764"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="OO372_001764"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6341281.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO372_001765"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="OO372_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="OO372_001766"
     CDS             complement(7411..8658)
                     /locus_tag="OO372_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6341282.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="OO372_001767"
     CDS             complement(8680..9006)
                     /locus_tag="OO372_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6341283.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO372_001768"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="OO372_001768"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6341284.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="OO372_001769"
     CDS             10395..11651
                     /locus_tag="OO372_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6341285.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /locus_tag="OO372_001770"
     CDS             11766..12092
                     /locus_tag="OO372_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6341286.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12233..13471)
                     /locus_tag="OO372_001771"
     CDS             complement(12233..13471)
                     /locus_tag="OO372_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6341287.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13807..15009
                     /gene="nupC"
                     /locus_tag="OO372_001772"
     CDS             13807..15009
                     /gene="nupC"
                     /locus_tag="OO372_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6341288.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15059..17247)
                     /locus_tag="OO372_001773"
                     /pseudo
     CDS             complement(15059..17247)
                     /locus_tag="OO372_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17456..17531)
                     /locus_tag="OO372_001774"
     tRNA            complement(17456..17531)
                     /locus_tag="OO372_001774"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17496..17498),aa:Ala,seq:ggc)
     gene            complement(17571..17646)
                     /locus_tag="OO372_001775"
     tRNA            complement(17571..17646)
                     /locus_tag="OO372_001775"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17611..17613),aa:Ala,seq:ggc)
     gene            17867..18226
                     /locus_tag="OO372_001776"
     CDS             17867..18226
                     /locus_tag="OO372_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6341289.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18228..18620
                     /locus_tag="OO372_001777"
     CDS             18228..18620
                     /locus_tag="OO372_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6341290.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OO372_001778"
     CDS             complement(18672..20087)
                     /gene="gltX"
                     /locus_tag="OO372_001778"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6341291.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20346..20421
                     /locus_tag="OO372_001779"
     tRNA            20346..20421
                     /locus_tag="OO372_001779"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20379..20381,aa:Val,seq:tac)
     gene            20467..20542
                     /locus_tag="OO372_001780"
     tRNA            20467..20542
                     /locus_tag="OO372_001780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20500..20502,aa:Val,seq:tac)
     gene            20589..20664
                     /locus_tag="OO372_001781"
     tRNA            20589..20664
                     /locus_tag="OO372_001781"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20622..20624,aa:Val,seq:tac)
     gene            20669..20744
                     /locus_tag="OO372_001782"
     tRNA            20669..20744
                     /locus_tag="OO372_001782"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20702..20704,aa:Lys,seq:ttt)
     gene            20775..20900
                     /locus_tag="OO372_001783"
     ncRNA           20775..20900
                     /ncRNA_class="other"
                     /locus_tag="OO372_001783"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21020..21361
                     /locus_tag="OO372_001784"
     CDS             21020..21361
                     /locus_tag="OO372_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6341292.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21352..22278)
                     /locus_tag="OO372_001785"
     CDS             complement(21352..22278)
                     /locus_tag="OO372_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6341293.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22368..23366
                     /locus_tag="OO372_001786"
     CDS             22368..23366
                     /locus_tag="OO372_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6341294.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23363..23581)
                     /locus_tag="OO372_001787"
     CDS             complement(23363..23581)
                     /locus_tag="OO372_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6341295.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="OO372_001788"
     CDS             complement(23583..25598)
                     /gene="ligA"
                     /locus_tag="OO372_001788"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6341296.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="OO372_001789"
     CDS             complement(25669..26655)
                     /gene="zipA"
                     /locus_tag="OO372_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6341297.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26885..27646
                     /gene="cysZ"
                     /locus_tag="OO372_001790"
     CDS             26885..27646
                     /gene="cysZ"
                     /locus_tag="OO372_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6341298.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27831..28802
                     /gene="cysK"
                     /locus_tag="OO372_001791"
     CDS             27831..28802
                     /gene="cysK"
                     /locus_tag="OO372_001791"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6341299.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29186..29443
                     /gene="ptsH"
                     /locus_tag="OO372_001792"
     CDS             29186..29443
                     /gene="ptsH"
                     /locus_tag="OO372_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6341300.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29488..31215
                     /gene="ptsI"
                     /locus_tag="OO372_001793"
     CDS             29488..31215
                     /gene="ptsI"
                     /locus_tag="OO372_001793"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6341301.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31256..31765
                     /gene="crr"
                     /locus_tag="OO372_001794"
     CDS             31256..31765
                     /gene="crr"
                     /locus_tag="OO372_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6341302.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7843 a         8384 c         7907 g         7662 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga
    12181 taaggcgttt acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc
    12241 ccagcgccgt ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc
    12301 agcctgtctg ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg
    12361 tgaaaatcag cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca
    12421 taaccagaat ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg
    12481 tgactgtacg acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct
    12541 gccccgccag tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa
    12601 agaccgttgc cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat
    12661 cggcaacacc agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca
    12721 gattgacaaa accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac
    12781 gttgttgacg cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat
    12841 gcggcataat cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta
    12901 aactcgggat caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact
    12961 cgacaatgta agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagtggtt
    13021 tttgcccgcg acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc
    13081 cctgcaacaa cgagacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg
    13141 ccaggtcggt tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac
    13201 gcggatagtg atcacgaatc tgctccgcca gattttttcc ggtggcaatc cctagtttgg
    13261 cagagaggat ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct
    13321 gatagccgaa gctggcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac
    13381 caatcgccgc aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc
    13441 cgctgctact ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta
    13501 tgtttcatgc tatgataaac gagaatgatt atcaaattca tttaaatgga ttgtggtgat
    13561 ttctctgata gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat
    13621 gtttaatgtt agcacattta cataactttc agcttccata cacaacatag cagaaatgta
    13681 tgacagatca ctatttttga agcctgtcac aggacgtcat tatagtgtgt gtcagatctc
    13741 gttttcctta accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga
    13801 gcaaatatgg accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca
    13861 ctgctggtaa gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt
    13921 atcgaagtgt tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa
    13981 ggcttctccg aaatgttcga aaaactgctc agatttgcca acgaagggac taacttcgtc
    14041 tttggtagca tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc
    14101 gtctttatct ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggtgattatc
    14161 cgcgcaattg gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac
    14221 gccgtcagct ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc
    14281 ggcaaaatct cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg
    14341 atgtccatcg ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg
    14401 gtactgaaca tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat
    14461 gccagtgaag aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg
    14521 ctgggtgaat acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc
    14581 ggctttatcg ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt
    14641 ggctacagca tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg
    14701 atgggtgttc cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt
    14761 tccaacgagt tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt
    14821 gctgaaggca tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt
    14881 atcgcaggtg cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt
    14941 ctgaaactgg tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg
    15001 gtgctgtaaa accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt
    15061 aatcagctaa tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct
    15121 tatgcaatag cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact
    15181 tcgctttcgc cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa
    15241 tatctttcac aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt
    15301 cgtagttggc atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca
    15361 gctgctcgat gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct
    15421 caagaatgac cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg
    15481 caatattctt ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt
    15541 tgtcgcaagg gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac
    15601 gcgcgctaag gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc
    15661 cgtcatattt cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg
    15721 gctgggcgta gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata
    15781 gcacctgttt agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat
    15841 gatgatgtgc gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg
    15901 tttcctgatt tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat
    15961 tgttccagtg aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg
    16021 tttccggccc gctcagcact aacagcagtt cactacccgg caattgatat aacttttcgt
    16081 tttcctgcat caacggcagc agcgtacggt aaattgatcg gatacagtga acgcgcatca
    16141 ttaagccgta atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaactctt
    16201 gcccgcctct tgctccggcg cttgttccaa cgcacggaag ttgggcagga gcgttaacgg
    16261 atcggtcagc gcctgcaaat gccagcggcg attaagccat tcactgcgat gataaatgcg
    16321 caccatatag agcaggcaaa cgctaaagga aatcagcacc gcaagaataa atgccagcga
    16381 atattcggtt tccacccctt gcaaaaagtt ctggttgtaa ttcagaaggc aaagcgttga
    16441 aacagccctg gtgagattta aaaacggata gcgaagctta ccgaccccga gggtaaagat
    16501 gatgaagaaa acgggtacca ggtaaccggc aataaagtcg ttttcataag gtgtgcacaa
    16561 cagaagtaga agcacgctta acgctgccag ccagcttaag gtaaatgcgc gtttctcttt
    16621 gcccaacgac ggcgcgatat ccctgcgcca caatatctgc gcaaagtggg gacttacaat
    16681 catgcgagtg agatagtaga aaagcatgtt gtaaataagc acagcggtga aaaggcttag
    16741 caaatcaacg accgtgaaaa tggcatccgc atcgccgaaa aaggtggata tctttagcgg
    16801 gaaatcaaag aagctcccca caagatacat gctgcatttg atgccgatcg gcgtcaccaa
    16861 accaagccag aacagacgtt gccagacata tcggctggtc aatccataac gccaacgcgt
    16921 ccccagctgc cagcgtaata ttgcgcaggc acacaacacg acaaacgtct ggctaccgag
    16981 catgaccgca gtctgtaaaa aagagagatg aaagttccac agattggtgc aaaacatccc
    17041 gcataaaacg ggaacgacac cgcgccagcc aaagataaac aagattgaca gcattacgca
    17101 cagtggcatc cacgccagaa agatataact ggaatggata atggcaagtg gcgaaataaa
    17161 acgggatagc tgaataagta ccacggtgag cgtaaacgct agtgtgaata tcttgatatt
    17221 ttttatcagg ttatgctcca caaacatgag tacactactc tcactgatcg gggttttcag
    17281 gcacacaaat aataagttta ttcgcatcgc gaagcaaaca gggggccagc ctgttgccga
    17341 tttttcatcc aatgatgctt tcaccaggcg gcactaaccc ggacgtagac tgtattacaa
    17401 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    17461 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    17521 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    17581 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    17641 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    17701 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    17761 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    17821 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    17881 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    17941 ggtacgcaag gagggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    18001 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    18061 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    18121 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    18181 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    18241 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    18301 accgttggat cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    18361 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    18421 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    18481 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    18541 agggaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    18601 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    18661 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    18721 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    18781 cccgccccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    18841 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    18901 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    18961 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    19021 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    19081 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    19141 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    19201 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    19261 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    19321 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    19381 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    19441 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    19501 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    19561 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    19621 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    19681 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    19741 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    19801 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    19861 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    19921 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    19981 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    20041 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    20101 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    20161 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    20221 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    20281 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc ggctccacac
    20341 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    20401 cgatcccgtc atcacccacc aacatacttt atgtagtctc cgccgtgtag caagaaattg
    20461 agaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt
    20521 tcgatcccgt catcacccac caatttctcg ccagctaaat ttcttgtaaa aatgtgaagt
    20581 accgaagtgg gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg
    20641 gttcgatccc gtcatcaccc accacttcgg gtcgttagct cagttggtag agcagttgac
    20701 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc
    20761 taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt
    20821 tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag
    20881 tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc
    20941 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
    21001 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
    21061 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
    21121 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
    21181 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
    21241 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
    21301 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
    21361 atacaacgga gccccttccc gaccagccat tagcgcattg ccctggtctc gtaccacgtc
    21421 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
    21481 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
    21541 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
    21601 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
    21661 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
    21721 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
    21781 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
    21841 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
    21901 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
    21961 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
    22021 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
    22081 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
    22141 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
    22201 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
    22261 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
    22321 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
    22381 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
    22441 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
    22501 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
    22561 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
    22621 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
    22681 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
    22741 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
    22801 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
    22861 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
    22921 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
    22981 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
    23041 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
    23101 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
    23161 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
    23221 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
    23281 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
    23341 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
    23401 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
    23461 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
    23521 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
    23581 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
    23641 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
    23701 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
    23761 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
    23821 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
    23881 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
    23941 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
    24001 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
    24061 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
    24121 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
    24181 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
    24241 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
    24301 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
    24361 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
    24421 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
    24481 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
    24541 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
    24601 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
    24661 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
    24721 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
    24781 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
    24841 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
    24901 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
    24961 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
    25021 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
    25081 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
    25141 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
    25201 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
    25261 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
    25321 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
    25381 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
    25441 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
    25501 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
    25561 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
    25621 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
    25681 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
    25741 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
    25801 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
    25861 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
    25921 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
    25981 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
    26041 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
    26101 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
    26161 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
    26221 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
    26281 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
    26341 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
    26401 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
    26461 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
    26521 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
    26581 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
    26641 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
    26701 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
    26761 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
    26821 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
    26881 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
    26941 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
    27001 tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc
    27061 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
    27121 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
    27181 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
    27241 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
    27301 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
    27361 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
    27421 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
    27481 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
    27541 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
    27601 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
    27661 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
    27721 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
    27781 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
    27841 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
    27901 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
    27961 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
    28021 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgta gctgccgctc
    28081 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
    28141 tgaaagcgtt aggtgcaaac ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa
    28201 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
    28261 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
    28321 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
    28381 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
    28441 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
    28501 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
    28561 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
    28621 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
    28681 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
    28741 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
    28801 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
    28861 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
    28921 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
    28981 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
    29041 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
    29101 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
    29161 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
    29221 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
    29281 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
    29341 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
    29401 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
    29461 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
    29521 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
    29581 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
    29641 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
    29701 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
    29761 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
    29821 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
    29881 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
    29941 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
    30001 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
    30061 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
    30121 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
    30181 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
    30241 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
    30301 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
    30361 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
    30421 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
    30481 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
    30541 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
    30601 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
    30661 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
    30721 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
    30781 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
    30841 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
    30901 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    30961 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    31021 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    31081 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    31141 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    31201 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    31261 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    31321 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    31381 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    31441 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    31501 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    31561 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    31621 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    31681 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    31741 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc ccatcg
//
LOCUS       DAJTVK010000038        31084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000038 DAJTVK010000000
VERSION     DAJTVK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.038"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..278
                     /locus_tag="OO372_001795"
     CDS             <1..278
                     /locus_tag="OO372_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6341303.1"
                     /translation="SVMCDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTR
                     FGKDMPGAMAEYGREYMKRKTKHGLRSNVTLTEDSEDWMPSCAIGHK"
     gene            582..827
                     /locus_tag="OO372_001796"
     CDS             582..827
                     /locus_tag="OO372_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341304.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            824..1246
                     /locus_tag="OO372_001797"
     CDS             824..1246
                     /locus_tag="OO372_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR6341305.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1906..3773
                     /locus_tag="OO372_001798"
                     /pseudo
     CDS             1906..3773
                     /locus_tag="OO372_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
     gene            4032..4313
                     /locus_tag="OO372_001799"
     CDS             4032..4313
                     /locus_tag="OO372_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341306.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            4953..5273
                     /gene="clpS"
                     /locus_tag="OO372_001800"
     CDS             4953..5273
                     /gene="clpS"
                     /locus_tag="OO372_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6341307.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            5304..7580
                     /gene="clpA"
                     /locus_tag="OO372_001801"
     CDS             5304..7580
                     /gene="clpA"
                     /locus_tag="OO372_001801"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6341308.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(7986..8073)
                     /locus_tag="OO372_001802"
     tRNA            complement(7986..8073)
                     /locus_tag="OO372_001802"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8037..8039),aa:Ser,seq:gga)
     gene            complement(8327..8545)
                     /gene="infA"
                     /locus_tag="OO372_001803"
     CDS             complement(8327..8545)
                     /gene="infA"
                     /locus_tag="OO372_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6341309.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(8830..9534)
                     /gene="aat"
                     /locus_tag="OO372_001804"
     CDS             complement(8830..9534)
                     /gene="aat"
                     /locus_tag="OO372_001804"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6341310.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="OO372_001805"
     CDS             complement(9576..11297)
                     /gene="cydC"
                     /locus_tag="OO372_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6341311.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="OO372_001806"
     CDS             complement(11298..13064)
                     /gene="cydD"
                     /locus_tag="OO372_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6341312.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="OO372_001807"
     CDS             complement(13187..14152)
                     /gene="trxB"
                     /locus_tag="OO372_001807"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6341313.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            14697..15191
                     /gene="lrp"
                     /locus_tag="OO372_001808"
     CDS             14697..15191
                     /gene="lrp"
                     /locus_tag="OO372_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6341314.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            15326..19393
                     /gene="ftsK"
                     /locus_tag="OO372_001809"
     CDS             15326..19393
                     /gene="ftsK"
                     /locus_tag="OO372_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6341315.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            19548..20159
                     /gene="lolA"
                     /locus_tag="OO372_001810"
     CDS             19548..20159
                     /gene="lolA"
                     /locus_tag="OO372_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6341316.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            20170..21513
                     /gene="rarA"
                     /locus_tag="OO372_001811"
                     /gene_synonym="mgsA"
     CDS             20170..21513
                     /gene="rarA"
                     /locus_tag="OO372_001811"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6341317.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            21604..22896
                     /gene="serS"
                     /locus_tag="OO372_001812"
     CDS             21604..22896
                     /gene="serS"
                     /locus_tag="OO372_001812"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6341318.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            23135..25579
                     /gene="dmsA"
                     /locus_tag="OO372_001813"
     CDS             23135..25579
                     /gene="dmsA"
                     /locus_tag="OO372_001813"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCR6341319.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            25590..26207
                     /gene="dmsB"
                     /locus_tag="OO372_001814"
     CDS             25590..26207
                     /gene="dmsB"
                     /locus_tag="OO372_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6341320.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            26209..27072
                     /gene="dmsC"
                     /locus_tag="OO372_001815"
     CDS             26209..27072
                     /gene="dmsC"
                     /locus_tag="OO372_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR6341321.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(27108..27734)
                     /locus_tag="OO372_001816"
     CDS             complement(27108..27734)
                     /locus_tag="OO372_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6341322.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            28049..29197
                     /locus_tag="OO372_001817"
     CDS             28049..29197
                     /locus_tag="OO372_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6341323.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            29407..30837
                     /locus_tag="OO372_001818"
                     /pseudo
     CDS             29407..30837
                     /locus_tag="OO372_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(30838..>31084)
                     /locus_tag="OO372_001819"
     CDS             complement(30838..>31084)
                     /locus_tag="OO372_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6341324.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         7870 a         7502 c         8287 g         7425 t
ORIGIN      
        1 cgtcggtaat gtgtgatggt ctgttagtgg gtaatgccga aattattccg ttcagtccgc
       61 gcaggtatct ttatcacgcc tatctggcat atatgagggc acacggattc ggtaaacctg
      121 taacactgac gcgcttcggt aaagatatgc cgggggcaat ggcggaatat ggcagggagt
      181 atatgaaacg gaaaacgaag cacggtttgc gttcaaatgt gaccctgaca gaggattcag
      241 aagactggat gccatcatgt gcaataggtc acaaatgacg atggaaaaaa ttaaacttat
      301 ggaataactg ttcaccactg ttcaccctgc aataaatgtc ttttatatca gtatattata
      361 gggtgaacag ttatttatga actgttcacc aaactattca ctgttcacct ttttgattct
      421 ttattgagct ttaagggtga acagtggtga acagttggtg aatagttttt gtgaaactgt
      481 taacccatta acatcatgaa taaaaaggga aaattgcaaa aggtgaacag gtgaagggtt
      541 aaaatgcaaa aattttattt tattgctgtg aggtaaagcc tgtgacaacg aagcacgcca
      601 aaaaaccaca atctcacgcc cttgatttga cagaacactg gctgagggtg gcgataaaaa
      661 tcatcgaccg caacgccggg gaaggatacg cgaaagcaca tcccgaactg ataagcgcat
      721 tcatgaccac gacggcggca aactttgcca cgctgacaga acgggagatt gccgaagcgg
      781 aacaggtgac aaccatcaac gttaaaaccg gagagcagac agcatgacag cacagatagc
      841 ggcttacggg cggctggtgg ctgacccgca gttaaagacc accagcaagg gtacgcaaat
      901 gacgatggcg agtatggcgg ttcccctgcc gtgcagccag gcagatgacg gaacggcgac
      961 gatatggtta tccgtcctgg cgtttggcag acaggctgac gcactggcaa agcaccagaa
     1021 aggcgagctg gtgagcgtgg cgggtaacat gcaggtaagc cagtggacag gccagaacgg
     1081 agaaacgcgg cagggctggc aggttatcgc agacagtgta atcagtgcca gaacggcgcg
     1141 accgggcggt aaaaaaggcc aacaggggca ggccactgat gcactgaaca gggccaaaca
     1201 acaggcgggg aatgatgatc cgtacgggga taacataccg ttttaagcaa cgagtgacag
     1261 aagccggagc aatccggctt ttttgtaggt actcctggtg ggggtggcct gtccacgggg
     1321 cgggcggttc gcggaaaaag gcgtattttt tgatttttat gtcaccatca ccaccagttt
     1381 aagttattga tatatggcaa aataaaaatt tttagtgtcg aattaggttg ttttttgttc
     1441 gacgccggaa cgttcccgaa agcatttaca aaaaacaggc gcaaaaaaaa gcgccccgat
     1501 ggtgccaccg gaggcgcttt gtcagcgaca aaggatactg ccactaccga aatgatgagt
     1561 cttaattgtg ctggcgtggc aataattagt caattctttt gctggtatga caaaaatcag
     1621 aaaacacata atctgatttt tagatagaaa atgatttatt taccagtttt atcgatcaat
     1681 aatgatgccc gttaatcata acggaggcag acttatgcca gagaacaaca gcagaaaacc
     1741 ggataaaagt gcaacagtgc acatagacgc cggaactatg gagaagctcg aacgctatca
     1801 gcagttcatt aaaagtcatc accctggaat gccagtaccc acgaaagggc agattgcgag
     1861 aagcgcggtt gaatactggt acagggcaac gttaggagcc tggctatgaa aacatggttt
     1921 tccattaagg ctatggctga tgttgtacat gtacgcattt atgacgagat cggcgggtac
     1981 ggtgtaaaag catcggcact tactgacgag atcaacgcgt gcggtaatgt gcctgaaatc
     2041 catcttcgca tccattcacc tggtggcgac atctttgaag ggctggctat ctataacgcc
     2101 ctgaaaaatc atccggcaaa gaaaattgta cacattgaag gcatggcggc ttctatggcc
     2161 tcgtttattg ccatgtgtgg cgatcacatc gttatgcctg aaaacgcgat gatgatgata
     2221 catgcccccc gtggtgttac tgccggagtg tcgggcgacg ttcgccgctt tgctgacctg
     2281 atggacaagc tgggcgacac gatggcggaa acctacgccg gaagaacggg caggagcaaa
     2341 caggaaatca ccgccatgat ggaggcggaa acctggatgg atggcaatga gtgtaaggct
     2401 aatggcttcg cagatgaggt tatacccgcg attacagcaa tggccagaat tgaatcaaaa
     2461 cgaatcggag atttttcgaa tatgccggaa aatattaaaa gcatgatcag ccagaaaact
     2521 ggcagcggag aacaggaacg actcaacggg atacgtgaat tgtttggcac gttcaatgga
     2581 agatataacg acctggctat aagttgtctt gctgattcag gctgtagcgt tgaggatgca
     2641 cgcgaacgcc ttttactcac tatgggtaaa gaatcgacac cgacaaacaa aaccaccccc
     2701 gcaaatcttt actacgcgta cacggataac ggcaacataa ccggcgatgc aatgcgtcag
     2761 gggcttaatg cgcgtctggg acatgaacag gcagaacgcg gcaaccctta cgccatgatg
     2821 agccttttcg atatggcaca ggcatcatta acccatcgcg gcataagcac aggcagctac
     2881 agcacacgct cgcagatagt aaacgcggca ttcacccaca gcagcagcga ttttacttat
     2941 atccttgctg gtggtgctga aaaatccgtg aacacagcgg cgaaacgttc cgccagtgga
     3001 cgaaaaaagg ttcattgtca aacttccatg aggcacgtcg tgttggcctg aatggcttct
     3061 ctacattaaa caaagtgccg gaaggcgcgg aatataaata catcaccacc agtgaccgtg
     3121 gcgaaccaat cgcgctggca acttacggaa atattttcag cattacccga caggcgatta
     3181 ttaacgatga cctgaatcag ttaacagtta tcccacaggc aatggggcga gcagccgcca
     3241 gaacggtggg taatctggtt catctggtgc tgacaggaaa cacaaagctt tctgacggta
     3301 taccgctgtt cagcaaaaaa cacggaaatg atattgataa cggagtcagc actacagggc
     3361 ttggcattgc acgccgtgca atgcgcacac agagggatga aaacggtgag gtgctgaata
     3421 ttgtgccaaa attccttctt gttcctgcgg ctctggaaga cagagcgttg caaatggtta
     3481 actcaaccac gcctttcggt gccgatacaa acagtggttc atataacccg taccacaaac
     3541 aactggatat tattgtcgat ccccgccttg atgaagtcag tgataagcaa tggtacatgc
     3601 tttcagcaca gggaacggac acaattgagg tggcttatct tgacggtaat gacgagcctt
     3661 accttgagca gcaggagggc tttattgttg acggtgtggc ctggaaggtc cgcattgatg
     3721 ctggcgttgc tgctctggat tatcgcggaa tggtcagagc gatttcttca tgagaacaag
     3781 gcggcagcag ccgccttttt tgcgggtcct cctggtgggg ggggcctgtc cacggggcgg
     3841 gtcggcgcgg aaaaaagcgc atttttgtgg ttttatcgtc atcatcatca tgtgtgtaac
     3901 ctgttgtttt taatgtagtt gatgcaaaaa agatgatgat tgtggttaat ttttgttcga
     3961 catcttttag cgtgacagat tctttacaaa aaatctgagc ttgttttctt aaccagcgcg
     4021 atgggggcac aatgacagaa gccgaaatac tgggattaat ccgccgcgtc gccggaatca
     4081 gccagcaggt tgacgaacag gccatgcaac cggacagcgt gaccgctgaa aattatgcgc
     4141 gtgtggtgga tgaggtaatg cgccgtgacg gtattgagct taacggcgtg gatatgcgca
     4201 acatacgaac cagagttctt gagttgctgg cataccgtcg ccgttctcaa caacggaggg
     4261 agagcgcgaa aaatacttac cagtggagga agccggagca cttacggcgg taactggttg
     4321 atattaccga aagtgcaaaa atgcacttag caactggtgg cgagttgcag atctgcaact
     4381 cgactatgaa actacggaaa ctacccgtag tttgggtagt aagagtaaca cccagatttt
     4441 ggggcttact cgcgataccc aaataaaggg tatcggtgga agaaatatcg tttctcatat
     4501 gtgagtaccg agggcggaat tccgccttcg gttacttatt gtgcgcatgc acagggaggg
     4561 gcgggtcaaa tctctgtacc ctgacgtctt ccggactgcc agccccatcg attttttata
     4621 cccgcgaaaa atgaaaaacg cttcacgctg gtgggcctga tgccgataac tggtttcatg
     4681 actgatagcc ggaaaatgat aacgattaaa gcgtgttgat ctttgttcgt ggttgttcga
     4741 tattgttcgt ggaacggtgt agttaatggt gtagttaatt ttactgtttt tgacgagctt
     4801 attgtttgca actcaatgaa aaataaaggc taataagcca aatgctataa atgatagtta
     4861 tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg
     4921 ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact
     4981 ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag
     5041 tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat
     5101 tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga
     5161 aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca
     5221 agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg
     5281 cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg
     5341 ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg
     5401 cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg
     5461 cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg
     5521 aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct
     5581 tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct
     5641 ttagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg
     5701 atgtggtgaa ttttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc
     5761 ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca
     5821 cgacgaacct gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga
     5881 aggagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg
     5941 tgggggaatc tggtgtcggt aaaaccgcga ttgcggaagg tcttgcctgg cgaattgttc
     6001 agggcgatgt gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc
     6061 tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc
     6121 agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg
     6181 cgggggcagc gtctggtggt caggttgatg cggctaacct aatcaaaccg ttgctctcca
     6241 gcggtaaaat tcgtgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga
     6301 aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcaatcgaag
     6361 aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt
     6421 ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatc aatgatcgtc
     6481 atctgccgga taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc
     6541 cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta
     6601 ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg aaaaacctcg
     6661 gcgatcgcct gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag
     6721 ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt
     6781 ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg
     6841 gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc
     6901 tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg
     6961 taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccggacg
     7021 tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca
     7081 aagcggactt ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta cgggaaactg
     7141 agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca
     7201 agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg tttgatcatc
     7261 tgtcaaccga cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc
     7321 tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga
     7381 aaggttacga ccgggcaatg ggcgctcgtc cgatggcgcg tgtcatccag gacaacctga
     7441 aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg
     7501 tcgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca
     7561 aggcggaagc agcgcattaa tctaattgtt aggtaggttg gtgaagtccg taatctcgaa
     7621 agaggttacg gactttttgt ttatggggtg gaacggtgaa aaccctattt ttggaggtga
     7681 aggtaatttg ttgataatta gtgctgctgg aaaaggtaag gataaaaaag ggtgacagca
     7741 ggaaaatgag atggttttgc tttattaaca acggactaaa cgtgtagtat ttgagttcac
     7801 tgccgtacag gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac
     7861 tgccgtacag gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac
     7921 tgccgtacag aaagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt
     7981 aaatatggcg gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca
     8041 ggcgtgctcc ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg
     8101 gtggcaacgg ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga
     8161 taattattgt ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt
     8221 acggacttat acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc
     8281 gaccttttac tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga
     8341 agacaatgcg gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg
     8401 tcaggatgcg gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac
     8461 cgttttctaa ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca
     8521 tttcaatatt gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac
     8581 cggcaagata atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa
     8641 acaggttcgg cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat
     8701 aagggagcgg tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc
     8761 ggggtaatga gcataaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc
     8821 cgaaaacatt catttttgtg gtgaaaacaa gcatcgtggc acccagaaat tattcggcaa
     8881 tcgtccgagg cgcatttgat tgagataatt cagataatcc cgacggggaa tttcgcaggc
     8941 accaagcgat gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgact
     9001 gataaattcc tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa
     9061 catggactcg ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc
     9121 atcttcatgc cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc
     9181 ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac aaccttcaat
     9241 gacctgacca aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt
     9301 catactacgg ctgatatgca gtgattcagg ccaaagcacc gcgcgaggat cgggcgacca
     9361 ccagaggatg ggggcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa
     9421 caggcgcgca gggctaagat cgccccccag agccagcagg ccgttaggct cacgtaatgc
     9481 gccttccggg gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc
     9541 aaaactccac gcaagtcgga tcgttcaata atagcttaca aaccctgctt aaactggtaa
     9601 taacgcccct gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg
     9661 tccatcacta ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac
     9721 accgttttct cacgcatcat ttctgcaagc aattcaagga tctggctttc ggttgtggca
     9781 tctaagcctt cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg
     9841 gcgatagcca gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa
     9901 ctgttgagac ctgcgtcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgac
     9961 agagcctcat cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta
    10021 aacagatgca ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg
    10081 ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca agcgcgggtc
    10141 agcagttgta acagtgttga tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc
    10201 ccggcgttta cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg
    10261 aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg
    10321 aaggtgactt ccggtttttg atccgttaaa tcagtgatcc gaacggcaga ggcaatgact
    10381 tgccccagat gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg
    10441 cagaagacaa acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac
    10501 gccatccaca gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac
    10561 aatgcggtca gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct
    10621 agttgcgtgc gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc
    10681 caggccgtca gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt
    10741 tttcccgcac gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc
    10801 agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga ctgtcaccac cataatcacc
    10861 acaaaagcgc ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca
    10921 tccgccacca cgcgattgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag
    10981 aggggcaaca atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg
    11041 tcgtgactta ccagacgttc aaaataacgc ccggcagtac gggtgattgc ggcaccacgc
    11101 acgcccgcag cgggtagcat atagttgaag ctgtacagtc cggcaccccc cgcaaccgct
    11161 gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtcaca
    11221 attgccagca caataccaag acttaacatc catttatgac gtttatacag tgccagatag
    11281 ggtagcaaag cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg
    11341 ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt
    11401 acccaaatga catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc
    11461 tggcgcagag aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg
    11521 ctggcagcgg gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc
    11581 gccacgcgct gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca
    11641 acgccttgtg gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt
    11701 aattcatgtt cgctggcatc aggtcgcgcc agtagtacgt tatcccgtaa tgttgctgcc
    11761 ggtaattgtg gattttgccc aacccaggag agatgtttac gccatgattc tggtgataaa
    11821 tcgcgtaatt ctatcccgtt gattcgtagc gatccctgat atgagagaaa accagaaagc
    11881 gcgttcaaca gtgaactttt acctgaaccg ctgcgaccaa ccaacacggc acgttggcct
    11941 gctggcaaag taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac
    12001 agatcctcgg cctcaatggt caccggatcg gtcgatgcta attccacctc gccacgctgc
    12061 ggatgggcga gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg
    12121 gctttagcat gataaaacgt accgagatcg cgtaatggct ggaaaaactc tggcgcaagg
    12181 atcagggcca gaaaacccgc agccagcgtc acgccagtat cgtagtgacc aaaatccagc
    12241 tcgccgagat aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa
    12301 aattcgagaa tgccggagga taaaaatgcc agccgtagca cttccattgt ccgttggcgg
    12361 aaatcttccg aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc
    12421 aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga
    12481 aagttacgtc ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt
    12541 aacggtgcag tgcccagcag aatgagcgcc gcagcccagt ttgaggggaa gattgccacc
    12601 acaatcagca acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa
    12661 tcatgcatat cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc aggtttaccc
    12721 tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag
    12781 cggatatgct gcccggcgtg ataacccacc cgttcgcgta accagaccgc ccatgcgcgc
    12841 agtacaaagg tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc
    12901 tccataatca tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg
    12961 ccgcttacaa agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc
    13021 tgttttaacc agcgggttaa ctctttttga cgagatttat tcattgcacg cttagcaggt
    13081 gagttatcag aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat
    13141 agtcgcaagg tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag
    13201 ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat gcagccagta ccggccgaag
    13261 taatggcctg gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc
    13321 tggtctgggt ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt
    13381 ccagctgccc ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca aacagaccgg
    13441 caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga
    13501 cacccatttg atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc
    13561 cgttctccac tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac
    13621 ggcggtgaat cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg
    13681 cggtattgcc gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg
    13741 ttgcacaagc agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc
    13801 gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat
    13861 tcagacggaa cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag
    13921 tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt
    13981 ttggatcacc aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca
    14041 tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc
    14101 ccgccgggcc tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc
    14161 catagttgtt ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt
    14221 atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc
    14281 cgtcattatc acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata
    14341 aacgcccctg ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca
    14401 atcccctggt gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc
    14461 ataaccatgc atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac
    14521 agacgccatt cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca
    14581 ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa
    14641 cagtgatgtt tcaggggcag acaggagtag ggaaggaata cagagagaca ataataatgg
    14701 tagatagcaa gaagcgcctt ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg
    14761 agttgcaaaa ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc
    14821 caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata
    14881 cggcgctgct taacccccat tatctggatg catcacttct ggtattcgtt gagattactc
    14941 tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag
    15001 aaattcagga gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc
    15061 cggatatgtc agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca
    15121 atgacacacg gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta
    15181 agacgcgcta acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc
    15241 catacagcaa cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa
    15301 aagcctgtaa cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac
    15361 attgacaaag ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc ttattgtcct
    15421 gtttgccgtc tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc
    15481 gcaaacggcc tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggttggc
    15541 agatacgctg ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg
    15601 cggttgttgg tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt
    15661 ttcgctacgc atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat
    15721 caacgctgac gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac
    15781 tacgctacaa ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc
    15841 agcgggcctg acgttgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg
    15901 ctggatttta aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt
    15961 cgatgaagat gagtatgaag acgacgaaga gtatgaagat gaaaatcacg gcaaacagca
    16021 tgaatcacgc cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga
    16081 aaaattcatt aatccgatgg ggcggcaaac agacgctgcg ttgttctccg gtaagcggat
    16141 ggatgatgac gaagagatta cctacactgc acgcggtgtg gctgccgacc ctgacgacgt
    16201 cctattttcg ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa
    16261 cggtgcgcca attaccgaac ctgtcgctgt agcagctgct gctaccacgg cggcacaaag
    16321 ctgggctgcg ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc
    16381 accttcgcaa cctacagtag cctggcagcc tgtaccgggt ccacaaacgg gagagccggt
    16441 tattgctcct gcaccggaag gttacccaca gcagccacaa tatgcgcagc ctgcagtgca
    16501 atataatgag ccgctgcaac aaccagtaca gccacagcag ccgtattatg cacctgcagc
    16561 tgaacaacct gtgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc
    16621 gtattacgcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca
    16681 gcaatccact tttgctccac agtctacata ccagactgag caaacttatc agcaaccagc
    16741 cgctcaggag ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc
    16801 tgaacccgtt gtagaagaga caaaacccgc gcgtccgcca ctttactatt ttgaagaagt
    16861 ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga
    16921 accggttaaa gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt
    16981 acctccagta gaaaccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac
    17041 actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg
    17101 tggaccgcgt ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat
    17161 ccgcgtgcca actcgccgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc
    17221 ggcggaagaa aaagcccgtg aagcccagcg caatcagtac gattctggcg atcagtacaa
    17281 cgatgatgaa atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca
    17341 gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga
    17401 tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc
    17461 cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat
    17521 gaaagcgttg ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt
    17581 acagcagccg caacaaccgg ttgcaccgca gcagcagtat cagcagccac aacagccggt
    17641 tgcgccacag cagcaatatc agcagccgca acagccggtt gcgccgcagc cacaatatca
    17701 gcagccgcaa caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc
    17761 gccgcagccg cagtatcaac agccgcaaca accggttgcg ccgcagcagc agtatcagca
    17821 gccacaacag ccagttgcgc cacaaccgca ggataccctg cttcatccgc tgttgatgcg
    17881 taatggcgac agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct
    17941 gacaccgccg ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctcg
    18001 cctggtggaa gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc
    18061 ggggccggtt atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cggcgcgcat
    18121 ttctaacttg tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga
    18181 agttattcct ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt
    18241 ttatctgcgc gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt
    18301 ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca
    18361 cttgttggtt gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct
    18421 gagcatgctt tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat
    18481 gctggagctt tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat
    18541 gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact
    18601 gatgtctgcg ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga
    18661 tcgcatgatg cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca
    18721 gcatccggtg ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct
    18781 gatgatgacg gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg
    18841 tgccgcgggt atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg
    18901 tctgattaaa gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc
    18961 acgtaccatt cttgatcagg ctggcgcgga atcactgctg gggatggggg atatgctcta
    19021 ctctgggccg aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga
    19081 agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat
    19141 cacttccgac agtgaaagcg aaggtggcgc gggcggtttc gatggcgctg aagaactgga
    19201 tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg
    19261 cgtacagcgt cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga
    19321 agcgcagggg attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggctccacc
    19381 gccgtttgac taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct
    19441 atcaactgaa aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag
    19501 agtagaggga actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg
    19561 ccatcacctg tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa
    19621 gcgatctgaa aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag
    19681 tgactgacgg tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc
    19741 caaacttatt caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta
    19801 aaacactgtg gttctataac ccgttcgttg agcaagctac ggcaacttgg ctgaaagatg
    19861 ccaccggtaa tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt
    19921 acaatatcaa acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc
    19981 tgaagcagtt caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg
    20041 agcaggacga tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg
    20101 cagcgaaatt taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga
    20161 ggcacctgag tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc
    20221 gcgcgtatgc ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg
    20281 gggaagccgt tgccgcgcgc tatcgaagcc gggcatctgc attctatgat cctctggggg
    20341 ccaccgggta ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat
    20401 gtggaacgta tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc
    20461 gcccggcaaa accgcaatgc aggtcgccgc actattcttt ttgttgacga agtgcaccga
    20521 ttcaacaaaa gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt
    20581 attggcgcaa ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc
    20641 cgtgtctatc tgttgaaatc cctgggtaca gaggatattg agcaagtact aactcaggcg
    20701 atggaagaca agacccgtgg ttatggtggt caggatattg ttctgccaga tgaaacacga
    20761 cgcgccattg ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg
    20821 atggcggata tggccgaagt caatgatagc ggtaagcggg tcctgaagcc tgaattattg
    20881 accgaaatcg ccggtgagcg aagcgcccgc tttgataaca aaggcgatcg cttttacgat
    20941 ctcatttccg cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctctactgg
    21001 tatgcgcgaa ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ttgcctggca
    21061 attgcttccg aagatgtcgg caatgccgac ccacgggcga tgcaggtggc aattgcggcc
    21121 tgggattgct ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt
    21181 gtttacctgg cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agctgcgctg
    21241 gcggatgctc gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg
    21301 aaattaatga aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac
    21361 gcttatgctg ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc
    21421 ccgacaaaca ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag
    21481 gatcaaaata gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact
    21541 gtatccctgt ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata
    21601 agcatgctcg atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca
    21661 cgccggggct ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta
    21721 ttgcaggtca aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc
    21781 caggcgaaag cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc
    21841 gaagagctgg atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc
    21901 gcgctgacca tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac
    21961 aacgttgaag tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac
    22021 gtgacgctgg gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt
    22081 tcccgctttg tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt
    22141 atgctggatc tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt
    22201 aaccaggaca cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat
    22261 actcgtccgc tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa
    22321 gttccgctga ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag
    22381 atgaccgccc acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt
    22441 ggtctgatcc gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa
    22501 gactcaatgg cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg
    22561 ggcctgccgt accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa
    22621 acttacgacc tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc
    22681 tccaacgttt gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac
    22741 aagaaaaccc gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg
    22801 gttgcagtaa tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg
    22861 cgtccgtata tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa
    22921 agcgcctgcg ggcgcttttt ttgtctccct ttgataccga acaataatta ctcttcactt
    22981 acacgtaata ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga
    23041 tgtatacaag cctatatagc gaactgctat agaaataatt acacaatacg gtttgttact
    23101 ggaatcaatc gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt
    23161 attggctgct gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc
    23221 aatggccagc agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc
    23281 cattccaaca aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag
    23341 tcgctgcccg ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa
    23401 taccggcgat gacaattacg acggcctgca ccaggttcgc gcctgcctgc gtgggcgttc
    23461 catgcgtcgc cgtgtctaca atccggaccg cctgaaatat ccgatgaaac gagtcggggc
    23521 gcgcggtgaa ggcaaattcg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac
    23581 caatatgcag cgcctgatca aagagtacgg caacgagtct atctatctga actatggcac
    23641 cggtacgctg ggcggcacca tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg
    23701 gctgatgaac tgctgcggcg gctatctgaa ccattacggc gactactcct ccgcgcaaat
    23761 tgcggaaggt ttgaactata cctacggcgg ctgggcagat ggcaacagcc cgtcggatat
    23821 cgaaaacagt aagctggtag tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg
    23881 cggtggggtg acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat
    23941 catcgatccg cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg
    24001 tccgggaaca gatgccgcac tggttaacgg tctggcgtac gtcatgatca ctgaaaacct
    24061 ggtggatcag gcattcctcg ataaatattg cgttggctac gatgagaaaa ccctgccagc
    24121 cagtgcgccg aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt
    24181 ggctaaaaca ccggaatggg cctcacaaat caccggtgtt ccggcagaca aaatcatcaa
    24241 actggctcgt gaaatcggca gtaccaaacc ggcgtttatc agccagggat ggggcccgca
    24301 gcgtcacgct aacggtgaaa tcgcaacccg tgctatctcg atgctggcga ttctgaccgg
    24361 taacgttggt attaatggag ggaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt
    24421 tgtccgtatg ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac
    24481 cgatgccatt gaacgtggcc cggaaatgac ggcgctgcgt gatggtgtac gcgggaaaga
    24541 taagctggat gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tggttaacca
    24601 gcattctgaa atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat
    24661 tgtggttatc gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga
    24721 ctgcaccgct tccgaacaga tggactttgc gctggatgca tcctgcggga atatgtctta
    24781 cgtgattttc aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat
    24841 gaccagcgaa ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtaccca
    24901 ggaagagtgg atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac
    24961 gtttgaagag ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt
    25021 tgcttataaa gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa
    25081 aattgagatt tattcgcagg cgctggctga cattgccgct acctgggaat tgccagaagg
    25141 cgatgtgatc gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgct
    25201 gaacaaacag tatccgctgc agcttacggg tttccactat aaatctcgcg ttcactcaac
    25261 ttacggcaac gttgatgtgc tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct
    25321 tgatgcgcaa aaacgcggta tcaacaacgg cgataaagtc aggatcttta acgatcgtgg
    25381 tgaagttcat attgaggcga aagtgacgcc acgaatgatg ccaggtgtgg tcgcactggg
    25441 tgaaggtgcc tggtatgacc cggatgcaaa acgtgtcgat aagggtggtt gtattaacgt
    25501 actgaccact caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt
    25561 tcaggttgaa aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt
    25621 ccagccgttg caccggttgc aaaacctgcg agctggcttg taaagactac aaagatttga
    25681 cgccagaagt cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata
    25741 acggtgtctg gcaccagaac gtgtttgcct actatctgtc gatttcgtgc aaccactgcg
    25801 aagatccggc ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt
    25861 ttgttgtggt cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt
    25921 atggcgcacc gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg
    25981 accgtgttgc ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg
    26041 atttcggccc tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt
    26101 tgccgcgagc tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc
    26161 cgaccgggga taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga
    26221 tggcatgaat ggccgctgat gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt
    26281 atcgttctgg ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt
    26341 atcgcctgca tgtttggctt atgggtgctg atgggcattg gctttatcgc ttctatgctc
    26401 catcttggtt caccgatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc
    26461 agtaacgaaa tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg
    26521 gcaatgctga aaaagctgtc accggcattg cgtacgcttt ggctggtggt gacgatggtt
    26581 cttggcgtcg tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg
    26641 acctggtaca gcatctggac gccgatgggc ttcttcctga cgatgttcat gggcggcccg
    26701 ttactggggt acctgttgct gagtctggct ggcgtcgata gctgggcgat gcgtctgctg
    26761 ccagccattt ccgtactggc actggtggtg agtggcgtgg tgtcagtgat gcagggcgca
    26821 gagctggcga ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt
    26881 gcgctgatgt cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca
    26941 cagctaaaag gttatcagcc tgcggttccg ctactatccg tctcattcat tctgcttctg
    27001 gcaggggaat taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc
    27061 gtcgcaagct aatcagtaac aaccggggtt tcggccccgg ttctctttta tttctgcttc
    27121 gttaacgtgt cataactggt catcaaatta cgataatcag gaatatggtt agagaacaac
    27181 gtcgccagtc cttcaatatc attacgccag tcacggtgca gctcgcaagc cacgccgaac
    27241 catgtcatca attgcgcacc tgcttgcgac atgcgatccc atgccgaatg ccgggtaatt
    27301 tcattaaagg tccctgacgc gtcagtcaca acaaacacat caaacccttc ttcaatggct
    27361 gaaagcgccg ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt
    27421 ttacctgtcg ctttgactgc ttttacaaaa tcttcgttat cccaggcgtt aatattgccc
    27481 gggcgagcaa tgtaaggtgc atcggggaat tgtgctttca gttctggaac taatgggccg
    27541 ttaggaccgg tttcaaaact ggtggtaaga atagttggta agttgaaata cttagccagg
    27601 tcacccagcg ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa
    27661 agtaaaccag cctggtgatc aacaagcaaa acagcagcat catttttatc aagacgaaca
    27721 tacggtttgg tcatcctctt ctcctttcga gagtcagtgg tacgcctgaa atcggtcaga
    27781 cgtgatgcat tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt
    27841 tttcgactga acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat
    27901 taatcatctc ttcagtatta gcgttatgaa tgagtaatat aaatcgataa taccgccgtt
    27961 gcgatatgcg cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga
    28021 aatttgaact tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt
    28081 gctgtctggc ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct
    28141 ttggctcgcc caggaacaca tgtccacatg gcaggtaggc gttgtcagct catcctattt
    28201 taccggcaac cttgtcggta cattgctgac agggtatgtc attaaacgca ttggttttaa
    28261 ccgcagctat tatctggcct ccttcatttt tgccgctggc tgtgccggcc ttggcctgat
    28321 gattggattc tggagttggt tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat
    28381 ttgggtagtc gttgagagcg cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt
    28441 gcttgctgca tacatgatgg tttattacgt gggaacgttt ttaggccagt tactggtcag
    28501 caaggtctca accgagctga tgagcgtatt gccgtgggtt acaggtttga cattggcagg
    28561 gatcttaccg ctgttgttta cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac
    28621 gtcaattacg gcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat
    28681 ctcaggaatc gttctgggat ctctatatgg cctgatgcct ctgtacctca atcacaaagg
    28741 ggtgagcaat gccagtattg gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg
    28801 acaatggccg attggacgtc tggcggataa gtttggtcga ctgctggttt tgcgtgttca
    28861 ggtctttgtc gtcattctcg gcagtattgc gatgcttagc caggcggcga tggccccagc
    28921 gttattcatc ctcggtgccg ctggctttac gctatatccg gtggcgatgg catgggcttg
    28981 cgagaaagtt gaacatcatc aactggtggc gatgaaccaa gccttactgt tgagctatac
    29041 tgtgggaagt ctgcttggcc cgtcatttac cgctatgcta atgcagaatt tctccgataa
    29101 tttattgttt atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgctgcgcaa
    29161 cgccggtcat acgccgaaac ccgttgctca cgtgtaaatg aattcaagca gagtgtgaac
    29221 ttactgtttc acactctgct tttttgtttc ttctatctga cttgctttat tccaaatttt
    29281 attcgtttaa aaataaaatg tgtagcaggt tataattttg catttcgcta tttccgcact
    29341 tcttatttgc cgcgcataat ccctcgtttt acagatgccc ctataatttt ggcgaaggat
    29401 ttgtctatgg ctgggaatgt tcaggaaaaa cagctgcgat ggtacaacat tgcgctgatg
    29461 tcttttatca ctgtctgggg ttttggcaac gttgttaata actacgccaa ccaggggctg
    29521 gtggttgttt tttcatgggt gtttatcttt gcgctctatt tcacacctta tgcgctaatt
    29581 gttggtcagt taggctcgac cttcaaagat gggaagggcg gggtcagtac ctggattaaa
    29641 cacacgatgg gacccggact ggcttatctc gccgcgtgga cctactgggt ggtgcatatt
    29701 ccctatctgg cacaaaaacc ctaggcaatt ctgattgcgc tcggttgggc gatgaaaggc
    29761 gacggttcgc taatcaaaga atattcagtc gtagcgttac aggggttaac gctggtgctg
    29821 tttatcttct ttatgtgggt tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct
    29881 gtggcaggga ttgctatgtt tgttatgtcg ctcctgtatg tggcgatggc ggtaaccgcg
    29941 cctgcaatta ctgaagtgca tattgcgacc acaaacatta cctgggaaac gttcattcct
    30001 catatcgact ttacctacat taccactatt tcaatgctgg ttttcgcggt tggcggagca
    30061 gagaagattt ctccttacgt taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg
    30121 atgttatgcc tggcggtgat ggttgcggtt tgtgccattc tgggctcgct ggcgatgggg
    30181 atgatgtttg attcgcgtaa tatcccggat gacttaatga ccaacggtca gtattacgcc
    30241 tttcagaagc tgggcgagta ttacaacatg ggtaatactt taatggtgat ttacgccatt
    30301 gcgaataccc tgggacaagt agcggcgctg gtattctcga ttgatgcccc gcttaaagtg
    30361 ctattaggtg atgctgacag caaatatatt ccagccagtt tatgtcgtac caacgcttct
    30421 ggtacgcccg ttaatggcta ttttctgacc ctggtactgg tggcgattct gattatgctg
    30481 ccgactctcg gcattggtga tatgaacaat ctctataaat ggctgttgaa ccttaattcg
    30541 gtagtgatgc cgctgcgtta tttatgggta tttgttgcat ttattgcagt cgttcgcctg
    30601 gcgcagaaat ataaaccaga atatgtcttt attcgtaata agcctctggc aatgaccgtc
    30661 gggatttggt gttttgcctt taccgccttt gcctgtttga cggggatctt cccgaaaatg
    30721 gaagccttca ctgcagagtg gaccttccag ttggcgctga atgttgcaac gccgtttgtg
    30781 ctggtaggat taggactgat attcccgctg ctggcgcgta aagcgaatag taaataatta
    30841 ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt tttacataat cgagaaatgc
    30901 gcgcaagccg caggacatat actggcggtt tgggtaatag atctggaagc ctggacgctc
    30961 tgtgatccag tcttccagta cacattctaa acgaccggta tcaaggtact ccttgatctg
    31021 ttcatagagc agatacccga taccagcacc catcaaaacg gcatccagtt cagcatcaac
    31081 atca
//
LOCUS       DAJTVK010000039        30002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000039 DAJTVK010000000
VERSION     DAJTVK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.039"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            38..580
                     /locus_tag="OO372_001820"
     CDS             38..580
                     /locus_tag="OO372_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6341325.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="OO372_001821"
     CDS             663..1364
                     /locus_tag="OO372_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6341326.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="OO372_001822"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="OO372_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="OO372_001823"
     CDS             3979..5049
                     /locus_tag="OO372_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6341327.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="OO372_001824"
     CDS             5061..5603
                     /locus_tag="OO372_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6341328.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="OO372_001825"
     CDS             5611..6126
                     /locus_tag="OO372_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6341329.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="OO372_001826"
     CDS             6092..6829
                     /locus_tag="OO372_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6341330.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="OO372_001827"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="OO372_001827"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6341331.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="OO372_001828"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="OO372_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6341332.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="OO372_001829"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="OO372_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6341333.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="OO372_001830"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="OO372_001830"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6341334.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="OO372_001831"
     CDS             12136..14043
                     /locus_tag="OO372_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6341335.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="OO372_001832"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="OO372_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6341336.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKATCPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="OO372_001833"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="OO372_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6341337.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="OO372_001834"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="OO372_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6341338.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="OO372_001835"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="OO372_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6341339.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="OO372_001836"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="OO372_001836"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6341340.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="OO372_001837"
     CDS             complement(18711..20471)
                     /locus_tag="OO372_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6341341.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="OO372_001838"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="OO372_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6341342.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="OO372_001839"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="OO372_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6341343.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="OO372_001840"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="OO372_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6341344.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="OO372_001841"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="OO372_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR6341345.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="OO372_001842"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="OO372_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6341346.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="OO372_001843"
     CDS             complement(26072..26518)
                     /locus_tag="OO372_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6341347.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="OO372_001844"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="OO372_001844"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6341348.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="OO372_001845"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="OO372_001845"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6341349.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="OO372_001846"
     CDS             complement(29281..29943)
                     /locus_tag="OO372_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6341350.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7733 a         7085 c         7879 g         7305 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcgacatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 ca
//
LOCUS       DAJTVK010000040        29940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000040 DAJTVK010000000
VERSION     DAJTVK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.040"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO372_001847"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO372_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6341351.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO372_001848"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO372_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6341352.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO372_001849"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO372_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6341353.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO372_001850"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO372_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6341354.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO372_001851"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO372_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6341355.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO372_001852"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO372_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6341356.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO372_001853"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO372_001853"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6341357.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO372_001854"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO372_001854"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6341358.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO372_001855"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO372_001855"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6341359.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO372_001856"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO372_001856"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6341360.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO372_001857"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO372_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6341361.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO372_001858"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO372_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6341362.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO372_001859"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO372_001859"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6341363.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO372_001860"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO372_001860"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6341364.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO372_001861"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO372_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6341365.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO372_001862"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO372_001862"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6341366.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMTEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO372_001863"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO372_001863"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6341367.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20761..21432
                     /gene="nfi"
                     /locus_tag="OO372_001864"
     CDS             20761..21432
                     /gene="nfi"
                     /locus_tag="OO372_001864"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6341368.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21475..22065
                     /locus_tag="OO372_001865"
     CDS             21475..22065
                     /locus_tag="OO372_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6341369.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22252..22524
                     /gene="hupA"
                     /locus_tag="OO372_001866"
     CDS             22252..22524
                     /gene="hupA"
                     /locus_tag="OO372_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6341370.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22537..23232
                     /locus_tag="OO372_001867"
     CDS             22537..23232
                     /locus_tag="OO372_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6341371.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OO372_001868"
     CDS             complement(23234..23659)
                     /gene="zraP"
                     /locus_tag="OO372_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6341372.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            23897..25273
                     /gene="zraS"
                     /locus_tag="OO372_001869"
     CDS             23897..25273
                     /gene="zraS"
                     /locus_tag="OO372_001869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6341373.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25270..26595
                     /gene="zraR"
                     /locus_tag="OO372_001870"
     CDS             25270..26595
                     /gene="zraR"
                     /locus_tag="OO372_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6341374.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OO372_001871"
     CDS             complement(26592..27881)
                     /gene="purD"
                     /locus_tag="OO372_001871"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6341375.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OO372_001872"
     CDS             complement(27893..29482)
                     /gene="purH"
                     /locus_tag="OO372_001872"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6341376.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7015 a         7804 c         8245 g         6876 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgcta cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agactccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacgccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacata
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgcttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gtaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaatccc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgactttttg gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggagctggtg
     4861 ccggaacgcc tgcgtggtga aactgcatct ttcgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgttg
     5101 gctaagctga gccagtctgg tcacaagcgt attgaaacgc tgttcaccaa cgacctggat
     5161 cacggcccgt atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acctgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttctg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggacatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccgg gtggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcattacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctttgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaggtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tttctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgtgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtttgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 accgacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggtg tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cactggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgccc gttgggccag tggttccaga
    13681 tagccactgg cggctggcga taacagcgcc atcatatcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gttctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg atcgctgaag gttttcatgc cgatgcctcc agaaatccag
    13861 tcagcgggct ggtggcatga gcaaaatgac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcta
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagggcc tgcgcggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcatttcggc ggctttcaga gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctctaa ttttaaccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tatttggcag cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg atttaccgtc
    14881 gaacagtcgg agttctcccg caggtgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 cgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtaccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcccgtgcaa ccgcatcttt taacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatatcgg gattcaactg tgtcagtcgc tgatggctga cctgcgattt
    15301 cgggcgatcg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cccgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtccaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgcgccag actgataccg ccaatcgcca cggtgggata atccgccagt cgctcaacat
    15721 gccgtgccag ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gccagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca ttgcgccgat ctttgatgcg tagctggagg gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttac gcaggtagat
    16201 ttctccgcct ctggcgcgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcacgta cttcctggct gattttcatc gagcagaatt tcggcccaca
    16321 catggagcaa aaatgggcga ctttacctga ctcttgcggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcctgga tgccctttcg ccaggtcagc
    16501 ggcgtggacg gcaatcttat aggtgataag cccctgctta acatcttctt tattaggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggc gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca
    16801 ggcaattttg gtcagttcgc ccagcgtatg cagctcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaaattg cgaccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagtcaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt agtacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgaggcgt acaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatgac ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcgcgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg cgacctacat ggtggaaggc
    20161 ggcagcagca aagcgttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctac tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gtgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcg
    20461 atggcagaaa ccggttgcga tgcgttgggc ctcgactgga caacggatat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacaat atcaccgcta aggagtgatt
    20761 atggatctcg cgtcattacg cgctcaacaa atcgaactgg cttcttctgt gatccgcgag
    20821 gatcgactcg ataaagatcc accggatctg atcgccggag tcgatgtcgg gtttgagcag
    20881 ggcggagaag tgacgcgagc ggcgatggtg ctgctgaaat atccctcgct tgagctggtg
    20941 gagtataaag ttgcccgcat cgccaccacc atgccttaca ttccaggttt tctttccttc
    21001 cgcgaatatc ctgcgctgct ggcagcgtgg gagatgctgt cgcaaaagcc ggatttagtg
    21061 tttgtcgatg gtcatgggat ctcgcatcct cgccgtcttg gcgtcgctag ccattttggt
    21121 ttgatggtgg atgtgccaac gatcggcgtg gcgaaaaaac ggctctgcgg taaattcgaa
    21181 ccgctctcca gcgaaccggg cgcgctggcc ccactgatgg ataaaggcga gcagctggcc
    21241 tgggtctggc gcagcaaagc gcgctgtaac ccgttgttta tcgctaccgg ccatcgggtc
    21301 agcgtggaca gcgcgctggc gtgggtacaa cgctgcatga aaggctatcg tctgccggag
    21361 ccaacgcgct gggcggacgc ggtggcctcg gaacgtccgg cgttcgtgcg ctatacagca
    21421 aatcagccct aattcaggta aactgcggat aatttccgta tttgagaact caacatgtta
    21481 caaaacccaa ttcatctgcg tctggagcgc ctggaaagct ggcagcatgt cactttcatg
    21541 gcttgcttat gcgaacgcat gtaccccaat tacgccatgt tctgccagca aaccggtttt
    21601 ggtgatgggc aaatttaccg tcgtattctc gatctcatct gggaaacgct gaccgttaaa
    21661 gacgcaaaag taaatttcga cagccaactg gagaaatttg aagaagcgat tccttcagcc
    21721 gacgatttcg atctgtacgg cgtttatccg gcaatcgatg cctgcgtggc gttaagtgaa
    21781 ctggtccatt cgcgtttgag tggtgaaacg ctcgaacacg cggtggaagt gagtaagacc
    21841 tccatcacga ccgttgcgat gctggaaatg actcaggctg gtcgcgaaat gagcgatgaa
    21901 gagcttaaag aaaacccagc tgtagagcaa gaatgggaca ttcagtggga aatattccga
    21961 cttttagctg agtgcgaaga acgcgatatc gagctgataa aaggccttag ggcagacctg
    22021 cgtgaggcgg gtgagagcaa tattggtata atttttcagc aataagacca gaaaacgtga
    22081 tttaacgcct gatttgtcgt acctggagtc ttccctttcg ccccccgtct ggtctacatt
    22141 tggggggcga aaaaaagtgg ctatcggtgc gtgtatgcag gagagtgcta ttctggcatt
    22201 tccgtcgcac tcgatgctta gcaagcgata aacacattgt aaggataact tatgaacaag
    22261 actcaactga ttgatgtaat tgcagagaaa gcagaactgt ccaaaactca ggctaaagct
    22321 gctctggagt ccactctggc tgcaattact gagtctctga aagaaggcga tgctgtacaa
    22381 ctggttggtt tcggtacctt caaagtgaac caccgcgctg agcgtactgg ccgcaacccg
    22441 cagaccggta aagaaatcaa aattgccgca gctaacgtac cggcatttgt ttctggcaag
    22501 gcactgaaag acgcagttaa gtaagattgc gtggcagtga acagttttaa cgaaggggtg
    22561 gtttcacccc ttttgtcttt ctggcgtcga tcattgatgc tggctggcgc tctgcttctc
    22621 actgcctgta gtcataactc ttcacttcct ccctttaccg ccagtggatt tgctgaagac
    22681 cagggcgcgg tacgcatctg gcgaaaagac agcggcgata atgtgcatct gcttgccgtg
    22741 tttagcccgt ggcgcagtgg cgataccacg acgcgagagt atcgctggca gggcgataac
    22801 ctcacgctca tcaatatcaa tgtttacagc aaaccgccgg tgaatattcg cgcgcgtttt
    22861 gacgatcgcg gtgatctgag ctttatgcaa cgtgaatccg atggggaaaa gcagcagctt
    22921 tctaacgacc aaatcgattt ataccgttat cgtgctgatc agatccgcca gattagcgat
    22981 gccttacgtc aggggagagt cgtgctgcgc caggggcgct ggcatgcgat ggaacagacc
    23041 gtgaccacct gcgaagggca aaccattaaa cctgatttag attcgcaggc gatagcgcat
    23101 atcgagcgcc gccagagccg ctcttctgtt gatgtcagcg tggcatggct ggaagcgccc
    23161 gaaggttcgc aattactgtt agtggcaaac tctgatttct gtcgctggca acccaacgag
    23221 aaaacgttct gatttaccag tggcccatac ccatatgacc gccaccaccg cagccgccgt
    23281 agcccatgcc cattccggct ccgcgcggaa tacccgcttc agccatcgcg atatctcgtt
    23341 tcacccgtaa ctcatctaac gactgacgca aattctccat ctctttggcg accgcgttaa
    23401 ttttgctgct atccggtggg ttcgcggcta acagggcatt gtattcataa cgcttcgtca
    23461 ccagttgctg ttgcagtgcg ctgctttgag cgtaaaagtc attatggatt ttctgccacg
    23521 ctgtctgttg ttcgctggtc aaaggcgcgg cattttgctg ccacataccg tgtccgccgt
    23581 gagcaaatgc agatgtcgat cccatcgcca ttgctgaaag cgccatcatt accagggcaa
    23641 ttttcgtgtt ccgtttcatg gttaatcctc cagtggttgt cttacttcgg gtattgcatc
    23701 ttccgtgcca acgatgaaac gctgatatga cgggtaatct ggcatgataa acgagtaaaa
    23761 atgactcgcc tgctgcgggt agcgagtcat ttttactcat tgaaacttaa gcctttgtgt
    23821 tacagcgcag ggtaagcgct gataaaagat ggcatgattt ctgctgtcag aaagggatga
    23881 gcaggcaaag aagaagatgc gttttatgca acgttctaaa gactccttag ctaaatggtt
    23941 aagcgcgatc ctccccgtgg tcattgttgg gctggtaggg ttgtttgcgg tgacggtgat
    24001 tcgcgattat gggcgcgaga ctgccgccgc cagacaaacg ctgctggaaa aaggcagtgt
    24061 acttattcgc gctcttgaat ccggctcgcg cgtcggcatg gggatgcgca tgcatcatgc
    24121 gcagcagcag gccttactgg aagaaatggc cgggcagcct ggagtacgtt ggtttgcggt
    24181 cacggatgaa caaggaacaa tcgtgatgca tagcaactcc ggcatggtgg gaaaacagct
    24241 ttattccccg caggaaatgc agcagttaca tccgggagat gaagaagcgt ggcggcggat
    24301 cgatagcgca gacggcgagc ctgttctgga aatttatcgc cagtttcaac cgatgtttgc
    24361 tgctggaatg caccggatgc gccatatgca gcagtatgcc gcgacaccac aagcaatttt
    24421 catcgctttc gacgccagta atattgtgag tgccgaagat cgtgagcaga gaaacaccct
    24481 gattatcctc ttcgccctgg cgacggtctt gctggcaagc gtattgtcat tcttctggta
    24541 tcgccgctat ttgcgctcgc gccagcttct acaagatgaa atgaagcgca aagagaagct
    24601 ggtggcgctg gggcatcttg cggcaggcgt tgcccacgaa atccgtaacc cactttcctc
    24661 gattaaagga ctggcgaaat actttgccga gcgcgcgcct gcagggggag aagcgcatca
    24721 actggcgcag gtgatggcga aagaggccga ccgtttaaac cgcgtggtaa gcgagctgct
    24781 ggaactggtt aagccaacgc atctggcttt gcaggcggtg gatctcaaca cgctgattaa
    24841 ccactcatta cagctggtaa gccaggatgc gaacagccgg gagatccagt tacgctttac
    24901 cgccaacgac acattaccgg aaattcaggc cgatccggac aggctgactc aggtcctgtt
    24961 gaatctctat ctcaatgcta ttcaggcgat tggtcagcat ggcgtgatta gcgtgacggc
    25021 cagcgaaagc ggcgcgggcg tgaaaatcag cgttaccgac agcggtaagg gaattgcggc
    25081 agatcagctt gatgccatct tcactccgta cttcaccact aaagccgaag gcaccggact
    25141 ggggctggcg gtcgtgcata atattgttga acaacacggt ggtacaattc aggtcgcaag
    25201 ccaggaggga aaaggcgcaa cgttcaccct ctggcttccg gtcactatta cgcgtaaggg
    25261 cccacaagga tgacgcacga taatatcgat attctggtgg tggatgatga cattagccac
    25321 tgcactattt tgcaggcttt actgcgcggc tggggctata acgtcgcgct ggcgaacagc
    25381 gggcgacagg cgctggagca ggtgcgggaa caggtttttg atcttgtgct ttgcgatgtg
    25441 cgaatggcgg agatggacgg cattgccacg ctaaaagaga tcaaagcgtt aaatccggcg
    25501 attccggtgc tgattatgac cgcatactcc agcgtcgaga cggcggtaga agcgttgaaa
    25561 accggggcgc tggattatct catcaagccg ctggatttcg ataacctgca ggcgacgctg
    25621 gaaaaggcgc tcgcgcatac gcacagtatt gacgctgaaa cgcctgcggt gtctgccagc
    25681 cagttcggta tggtcggtaa aagcccggcg atgcaacacc tgctcagtga aatcgccctc
    25741 gtcgcgccat cggaagccac ggtgctgatc cacggcgatt ccggcaccgg taaagagctg
    25801 gtcgccaggg cgattcacgc cagtagcgca cgtagcgaaa aaccgctggt aacgctcaac
    25861 tgtgcggcac tcaacgaatc cttgctggaa tctgaattgt tcggtcacga aaaaggggcg
    25921 ttcactggag ccgatagacg ccgggaaggg cgctttgttg aggcggacgg cggcacgctg
    25981 tttctcgatg aaattggcga tatctcgccg atgatgcagg tgcgtctgct gcgtgcgatt
    26041 caggagcgcg aagttcagcg tgtcggtagc aaccagacta tctcggttga tgtccggttg
    26101 attgcggcga cccatcgcga tcttgccgca gaggtgaatg ccgggcgttt tcgccaggat
    26161 ctctactatc gcctgaatgt ggtggcgatt gaagtaccat cgctgcggca acggcgggaa
    26221 gatatccctc tgctggctgt ccattttctt cagcgctttg ccgagcgtaa tcgcaaggcg
    26281 gtaaaaggct ttacgccaca ggcgatggat ttgctgatcc actacgactg gccgggaaat
    26341 attcgtgaac tggaaaacgc ggtggaacgg gcggtggtgc tgctgaccgg ggaatatatt
    26401 tccgaacgcg agctgccgct ggcgattgcc ggtacgccga tcccactggg gcaaagtcag
    26461 gatattcagc cgctggtgga agtcgaaaaa gaagtgattc tggcggcact ggagaaaacg
    26521 ggcggcaaca aaaccgaagc cgcccgtcag ttagggatca cgcgcaaaac gctattggca
    26581 aaactgtcgc gttagttctg ctcgcgttcg atagcgcgcc agccgatatc tttccggcag
    26641 aagcagtcgt cccagtgaat atcggtcatt aaggcataag cgcgtttctg cgcttctgcc
    26701 acggtatgac ccagcgcggt gacgcacagt acgcgcccgc cgctggtcac tacctgctcg
    26761 ttatccgcca gtttcgtgcc cgcgtggaac actttgccgc ctgccacttc ttccagcggc
    26821 aggccgtgga tcacatcgcc ggtgcggtaa tcgcccggat atccacccgc agccatcacc
    26881 acgccgagag aagcacgctc atcccactct gacgttttct cgtccagttt gccttcacag
    26941 gccgccaggc agagctcaac cagatcggac ttcatgcgca gcataatcgg ctgggtttct
    27001 ggatcgccaa agcggcagtt aaattcgata accttcggat tgccctgttt gtcgatcatc
    27061 aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg ccgccatgcc tttcacggtt
    27121 ggccagatga tacgttccat ggtgcgctga tgaacttcat cggttactac cggcgcgggg
    27181 gagtaagcgc ccatcccgcc cgtgtttggt ccggtatctt tatcgcctac gcgtttgtga
    27241 tcctggctgg tggccatcgg cagcacatgc tcgccgtcta ccatcacgat aaagctcgct
    27301 tcttcgccgt cgaggaactc ttcgataacg atgcgatgac ccgcgtcgcc aaaagcgttg
    27361 cccgccagca tatcgtgaac agccgcttcg gcttcttcca gcgtcatcgc cacgataacg
    27421 cctttcccgg ctgccagacc gtccgcttta atgacgatcg gcgcaccttt ctcacgcaga
    27481 tacgccagcg caggttctac ctcggtgaag ttctggtatt ccgctgtagg gatcttatgg
    27541 cgggccagga aatctttggt aaacgctttc gaaccttcca gttgcgcggc acctgcggtt
    27601 gggccgaaga ttttcatccc ggcggcgcgg aaggtatcga ccacgccttt caccagcggc
    27661 gcttccgggc cgacgatggt cagatcaatc ttttcgtttt gtgcgaaatc caacagcgcc
    27721 gggatatcgg tcacgccgat agcaacgttt tgcagcgcgg gttccagcgc agtgcctgca
    27781 ttacccggag caacaaaaac agtttcaacc agcggcgact gcgccgcttt ccaggccagc
    27841 gcgtgctcgc gcccgccgtt accaatcact aatactttca tctattgctc cattaatggc
    27901 ggaagtggcg catgtcggtg aagagcatcg caataccgtg ctcgtcggcg gcggcaatca
    27961 cttcgtcatc acggatagaa ccgccaggct ggattacgca ggtcacgcct gcagcggcgg
    28021 cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga agccatcgag gaacctttca
    28081 cttccaagcc ttcatcggca gctttaatac cggcgatttt cgcggagtac acgcggctca
    28141 tctggcccgc gccaatcccg atggtcatat tgtttttggc atagacgata gcgttggatt
    28201 tcacgaactt cgccactttc cagcagaaca gcgcatcacg cagttcctgt tcggtcggct
    28261 gacgttgggt gacgacgcgc agttcttccg caccgaccat gcccaggtca cgatcctgaa
    28321 ccagcagacc gccgttcacg cgtttgaaat caagacccgg aacacgctcg ccccactgac
    28381 cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat tttcagggct tcttcgctgg
    28441 cggaaggcgc aataatcact tcgacaaact gacgagaaat gatggcctgt gcggtttccg
    28501 catccagctc gcggttaaag gcaatgatgc cgccgaatgc ggaggttggg tcggttttgt
    28561 acgcgcgatc gtaagcatca agaatggaat tgccgatagc cacgccgcaa gggttggcgt
    28621 gcttcacaat cacacatgcc ggctcggcga actctttcac gcactccagc gccgcatcgg
    28681 tatcggcgat gttgttataa gagagggctt taccctgaac ctgggttgcg gtagcaacgg
    28741 aggcttcttt cacattctct tctatataga aggcagcctg ctggtggctg ttctcgccgt
    28801 aacgcatatc ctgcttctta atgaagttca ggttcagcgt gcgtgggaag cgaccggcgg
    28861 cttctttgct ttcaccgtgg taagccggaa ccatgctgcc gaagtagttg gcaatcatgc
    28921 tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag gtcgaaacgg gttgcaagcg
    28981 tcagcgatcc ttcgttgtca tccatctctt taataatggc gtcgtagtcg ctgctcttca
    29041 ccacgattgc gacatcttta tggttcttag cggcggagcg caccatggtc gggccgccga
    29101 tatcgatgtt ctcaaccgca tcttccagcg agcagccttc acgagcaaca gtctgggcga
    29161 acggatacag gttaacaacc accatatcga taggctggat ctgatgttct tccataatgg
    29221 catcgtcctg gccgcgacgg cccagaatgc caccatgtac tttcggatgc agggtcttca
    29281 cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga aacttcggtt accggcagac
    29341 ctttttctgc taacagacgg gcagtgcccc ctgtagacag cagctccaca ccgcgtgcgg
    29401 aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga aacactgagc agagcgcggc
    29461 ggactggacg acgttgttgc atggtaaatc ccctggattt gactattaca gagagcgtta
    29521 gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg gggcatcctt atttttcgcc
    29581 cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt ttctctttca atggtgatca
    29641 caattttgac agtggttacc gtgggaaaaa tacagaaatt acattgatga ttgtggataa
    29701 ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg aatgcagcag tcagtcattt
    29761 ttctgcaatt tttctattgc ggcctgcgga gaaatcccta taatgcgcct ccatcgacac
    29821 ggcggatgtg aatcacttca cacaaacagc cggttcggtt gaagagaaaa atcctgaaat
    29881 tcagggttga ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgcgctaaa
//
LOCUS       DAJTVK010000041        29549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000041 DAJTVK010000000
VERSION     DAJTVK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.041"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..690
                     /gene="dgt"
                     /locus_tag="OO372_001873"
     CDS             <1..690
                     /gene="dgt"
                     /locus_tag="OO372_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HCR6341377.1"
                     /translation="LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSR
                     SNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDL
                     LKLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKR
                     FPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAW
                     DEYRRLMAVEQ"
     gene            820..2244
                     /gene="degP"
                     /locus_tag="OO372_001874"
     CDS             820..2244
                     /gene="degP"
                     /locus_tag="OO372_001874"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6341378.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2399..3556
                     /gene="cdaR"
                     /locus_tag="OO372_001875"
     CDS             2399..3556
                     /gene="cdaR"
                     /locus_tag="OO372_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR6341379.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3645..4031)
                     /locus_tag="OO372_001876"
     CDS             complement(3645..4031)
                     /locus_tag="OO372_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6341380.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OO372_001877"
     CDS             complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="OO372_001877"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR6341381.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OO372_001878"
     CDS             complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="OO372_001878"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6341382.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OO372_001879"
     CDS             complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="OO372_001879"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6341383.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8649..9443)
                     /gene="map"
                     /locus_tag="OO372_001880"
     CDS             complement(8649..9443)
                     /gene="map"
                     /locus_tag="OO372_001880"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6341384.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9811..10536
                     /gene="rpsB"
                     /locus_tag="OO372_001881"
     CDS             9811..10536
                     /gene="rpsB"
                     /locus_tag="OO372_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6341385.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10794..11645
                     /gene="tsf"
                     /locus_tag="OO372_001882"
     CDS             10794..11645
                     /gene="tsf"
                     /locus_tag="OO372_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6341386.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11792..12517
                     /gene="pyrH"
                     /locus_tag="OO372_001883"
     CDS             11792..12517
                     /gene="pyrH"
                     /locus_tag="OO372_001883"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6341387.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12809..13366
                     /gene="frr"
                     /locus_tag="OO372_001884"
     CDS             12809..13366
                     /gene="frr"
                     /locus_tag="OO372_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6341388.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13458..14654
                     /gene="ispC"
                     /locus_tag="OO372_001885"
     CDS             13458..14654
                     /gene="ispC"
                     /locus_tag="OO372_001885"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6341389.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14843..15601
                     /gene="ispU"
                     /locus_tag="OO372_001886"
                     /gene_synonym="uppS"
     CDS             14843..15601
                     /gene="ispU"
                     /locus_tag="OO372_001886"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6341390.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15614..16471
                     /gene="cdsA"
                     /locus_tag="OO372_001887"
     CDS             15614..16471
                     /gene="cdsA"
                     /locus_tag="OO372_001887"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6341391.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16483..17835
                     /gene="rseP"
                     /locus_tag="OO372_001888"
     CDS             16483..17835
                     /gene="rseP"
                     /locus_tag="OO372_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6341392.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17865..20297
                     /gene="bamA"
                     /locus_tag="OO372_001889"
     CDS             17865..20297
                     /gene="bamA"
                     /locus_tag="OO372_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6341393.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20419..20904
                     /gene="skp"
                     /locus_tag="OO372_001890"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20419..20904
                     /gene="skp"
                     /locus_tag="OO372_001890"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6341394.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20908..21933
                     /gene="lpxD"
                     /locus_tag="OO372_001891"
     CDS             20908..21933
                     /gene="lpxD"
                     /locus_tag="OO372_001891"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6341395.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22038..22493
                     /gene="fabZ"
                     /locus_tag="OO372_001892"
     CDS             22038..22493
                     /gene="fabZ"
                     /locus_tag="OO372_001892"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6341396.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22497..23285
                     /gene="lpxA"
                     /locus_tag="OO372_001893"
     CDS             22497..23285
                     /gene="lpxA"
                     /locus_tag="OO372_001893"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6341397.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23285..24433
                     /gene="lpxB"
                     /locus_tag="OO372_001894"
     CDS             23285..24433
                     /gene="lpxB"
                     /locus_tag="OO372_001894"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6341398.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24430..25026
                     /gene="rnhB"
                     /locus_tag="OO372_001895"
     CDS             24430..25026
                     /gene="rnhB"
                     /locus_tag="OO372_001895"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6341399.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25063..28545
                     /gene="dnaE"
                     /locus_tag="OO372_001896"
     CDS             25063..28545
                     /gene="dnaE"
                     /locus_tag="OO372_001896"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6341400.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28558..29517
                     /gene="accA"
                     /locus_tag="OO372_001897"
     CDS             28558..29517
                     /gene="accA"
                     /locus_tag="OO372_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6341401.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7121 c         8160 g         7109 t
ORIGIN      
        1 cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca tcatttgcac
       61 gaggcgtggg gtcagcatga gaaaggttcg ctcttttcgc tagtggttga aaatgcctgg
      121 gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt tatgtattta
      181 cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat tgataatctg
      241 cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag cgaatgcagc
      301 gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca tccggatgtc
      361 gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat ttatcgtcct
      421 ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg ggtgaaacgt
      481 ttccctattg agtcgcgctt attccacaaa ctctcgacgc gccatcggct ggcctatgtc
      541 gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga atattattac
      601 cgttgccgcc tactgcagga ttatatcagc ggtatgaccg acctctatgc gtgggatgaa
      661 taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga acaataaatt
      721 tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat ctgaagaaca
      781 cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac cacattagca
      841 ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc aacggcggct
      901 gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat gctcgaaaag
      961 gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa tacgccgcgt
     1021 atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca ggaaggttca
     1081 ccgtttcaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg cggccagcaa
     1141 cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc
     1201 accaacaacc atgttgttga taacgcgacg gtgattaaag tccagctgag cgatggtcgt
     1261 aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct gatccaaatc
     1321 cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact gcgcgtgggt
     1381 gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac ttccgggatt
     1441 gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt catccagacc
     1501 gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa cggcgaactg
     1561 atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat cggttttgct
     1621 atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa
     1681 cgcggtgagc tggggattat ggggactgag ctgaactccg aactggccaa agcgatgaaa
     1741 gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa
     1801 gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat cagcagcttt
     1861 gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac cctgggctta
     1921 ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag ccagaatcag
     1981 gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa caaaggcaaa
     2041 gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca gatcggcctg
     2101 aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg
     2161 cgtaaagtgc tcgacagcaa accgtctgtg ctggcactga acattcagcg cggcgacagc
     2221 accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg ggaaggggtt
     2281 ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc tttgggcatt
     2341 tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg gaggacttat
     2401 ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat
     2461 catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca gcggcgatcg
     2521 tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt
     2581 cgatatcgat gacgcggtag cacgtcatct gcacggtgtg cggcagggga ttaatctacc
     2641 gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct
     2701 gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt
     2761 gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga acctgattca
     2821 ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga ttgatctcaa
     2881 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg tggacagcgc
     2941 aatggcggag ttacaacaac tgcaaaatgc gctgactacg cccgagcgta ataatctggt
     3001 ggcaattgtt tcgctgaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
     3061 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
     3121 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
     3181 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
     3241 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
     3301 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataatgg
     3361 cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc tggctacgtc
     3421 aaaggcgctg tttattcatc gtaatactct ggagtatcgg cttaatcgta tatcggaact
     3481 gaccgggctt gatttgggta attttgatga caggttgctg ctgtatgtgg cgttacaact
     3541 ggatgaagag cggtaggtta tgcgttaagg tcgaggcggc gctggctcat tcccctcagc
     3601 ccgaagggta gaggggaatg tccgagttaa ccgtcagttc ggcattattt acgcgttagt
     3661 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
     3721 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga
     3781 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
     3841 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttcacgctc
     3901 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
     3961 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
     4021 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
     4081 catggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
     4141 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg ttctacacca
     4201 gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa ctgcccacgc
     4261 cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg taacgtttat
     4321 cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg ccgtgagtat
     4381 ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg tcgggattat
     4441 gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg cattgtccgg
     4501 cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg atcaccgtag
     4561 cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt aagtgatttt
     4621 tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc gcacattcgg
     4681 caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca aataatacgt
     4741 aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca atcgcgtcag
     4801 aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa agaattttga
     4861 atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca aaccagccta
     4921 gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg gcagtagcct
     4981 gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg gcagcgtcgt
     5041 gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc
     5101 cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat
     5161 cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga
     5221 tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatggaaa
     5281 tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg ccaatgaagc
     5341 agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg ccgatgccca
     5401 cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg
     5461 tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca
     5521 cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca acgcggaagc
     5581 cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt
     5641 agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg
     5701 cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg
     5761 caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg gtgtctgcgt
     5821 tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct
     5881 gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg
     5941 cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag
     6001 cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat
     6061 gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt
     6121 cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggttggcaaa aacgtcactt
     6181 cggtttcaac agtaaagtga cgtagtttag ccggttgacg acgcggctgt ggtggctgcc
     6241 agctactttg tgtcagtacc tgctccagac caaaccgaat aacctcatga cgatctgcgg
     6301 atagcgggct accatcgggt tccagcacga tgaaggtatc catcgccata ccgtcgcgag
     6361 tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg
     6421 cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag
     6481 cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat
     6541 gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa
     6601 tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt
     6661 ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct
     6721 tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt
     6781 cgttggtggc gcaaatgtca gccactgtca ggcataccag atagtgcaga cgattttccg
     6841 tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg
     6901 ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg
     6961 agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt
     7021 cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg
     7081 acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg
     7141 caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga
     7201 acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca
     7261 cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc
     7321 tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg
     7381 catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca
     7441 tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca
     7501 ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt ttttcgtcgg
     7561 cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga
     7621 cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac
     7681 cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg cgattatcgt
     7741 aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt
     7801 ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca tccagcgatg
     7861 ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc aagccgccag
     7921 ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc tgatggcgct
     7981 ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa
     8041 aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga
     8101 ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc
     8161 gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt
     8221 tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa
     8281 gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct
     8341 ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg
     8401 cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga
     8461 aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg
     8521 gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg
     8581 tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg
     8641 gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc
     8701 agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg
     8761 cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg
     8821 ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg
     8881 gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccgcaa
     8941 tattcacgaa cgacggagaa gccttctgct tcgataaact tctgaatcgc cgcaccgatt
     9001 tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg
     9061 atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta
     9121 tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc
     9181 agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg
     9241 gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc attaacaatg
     9301 taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg
     9361 atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct
     9421 ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat
     9481 atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg
     9541 ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg ctgtacacag
     9601 cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat
     9661 ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac
     9721 gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat
     9781 aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc
     9841 aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc
     9901 atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc
     9961 aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt
    10021 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc
    10081 ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc
    10141 atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc
    10201 aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt
    10261 atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac
    10321 attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac
    10381 tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg
    10441 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct
    10501 tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa
    10561 tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt
    10621 gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag ggggagagcc
    10681 cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact
    10741 tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg
    10801 aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt
    10861 gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta
    10921 agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca
    10981 aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag actgacttcg
    11041 ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg
    11101 gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg
    11161 tagcgaaaat tggtgagaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc
    11221 tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg
    11281 aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa ttcatcaaac
    11341 cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga
    11401 tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat
    11461 tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg
    11521 gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtggggg
    11581 aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt
    11641 cttaattatc gaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt
    11701 cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca
    11761 tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa
    11821 acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga
    11881 tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca
    11941 ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg
    12001 tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc
    12061 aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt
    12121 gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg
    12181 tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg
    12241 cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt
    12301 gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag
    12361 cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc tggctcgtga
    12421 ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt
    12481 aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg
    12541 taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt attgttgcag
    12601 gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg
    12661 agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt
    12721 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    12781 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    12841 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    12901 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    12961 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga
    13021 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    13081 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    13141 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    13201 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    13261 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    13321 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    13381 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    13441 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    13501 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    13561 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    13621 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    13681 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    13741 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    13801 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    13861 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    13921 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    13981 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    14041 tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac tggtcgatgg
    14101 gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    14161 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    14221 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    14281 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    14341 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    14401 atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat
    14461 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    14521 atatcgctgc gttgaattta tctgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    14581 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    14641 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    14701 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    14761 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    14821 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    14881 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    14941 gcaagggagg attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    15001 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    15061 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    15121 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    15181 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagctgggaa
    15241 taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    15301 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    15361 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    15421 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    15481 ttactttaca gatgttctct ggcctgattt cgatgaacaa gactttgaag gggcgttaaa
    15541 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    15601 atggggggcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    15661 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg
    15721 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    15781 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    15841 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt tgggttggtg
    15901 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    15961 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    16021 ggcgttacgg gcctggcact atgacgagaa tcattacagt ggcgcaatat ggctgctcta
    16081 tgtcatgatc ctggtatggg gcgctgactc cggcgcatat atgtttggca aattgtttgg
    16141 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    16201 actagcgacc gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    16261 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    16321 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    16381 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    16441 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    16501 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    16561 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    16621 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatggccct gattccgctg
    16681 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    16741 catgccttca ataataaatc tgttggccaa cgagcggcga ttattgccgc aggtccggtt
    16801 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    16861 gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    16921 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    16981 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    17041 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    17101 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    17161 gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    17221 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    17281 aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca
    17341 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    17401 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    17461 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    17521 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    17581 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    17641 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    17701 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    17761 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    17821 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    17881 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    17941 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggca ctcctcagta
    18001 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    18061 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    18121 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    18181 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    18241 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    18301 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    18361 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    18421 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    18481 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    18541 tgcgcagcta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    18601 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa cattaccgaa ggcgatcagt
    18661 acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    18721 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    18781 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    18841 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    18901 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    18961 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    19021 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    19081 cgggtagccc ggaccaggta gatgtagtct acaaggtgaa agaacgtaac accggtagct
    19141 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggcgtgcaac
    19201 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    19261 agacctatgc tgaattgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    19321 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    19381 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    19441 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    19501 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aacagcttca
    19561 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    19621 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    19681 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    19741 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    19801 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    19861 atacaattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    19921 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    19981 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    20041 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    20101 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    20161 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    20221 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    20281 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    20341 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    20401 gatggtaagg agtttattgt gaaaaagtgg ttattagcgg caggtctcgg tttagcactg
    20461 gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca atatgggcag cctgttccag
    20521 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    20581 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    20641 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    20701 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    20761 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    20821 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    20881 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    20941 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    21001 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    21061 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    21121 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    21181 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    21241 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    21301 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    21361 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    21421 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    21481 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    21541 tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg cgtgatcatt
    21601 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    21661 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    21721 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    21781 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    21841 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    21901 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    21961 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    22021 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    22081 aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag
    22141 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    22201 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    22261 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg
    22321 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    22381 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgatg
    22441 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    22501 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcaattggcg
    22561 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    22621 ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga
    22681 tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga
    22741 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    22801 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    22861 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    22921 cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    22981 tctgcatcat tggtgcgcac gtgatggttg gcggctgctc cggtgtggcg caggacgtcc
    23041 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    23101 tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga
    23161 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    23221 catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    23281 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    23341 tatcctgggg gccggtttaa tccgcgctct gaaagaacat gtgcccaacg cccgctttgt
    23401 tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcttggtacg aaatggaaga
    23461 actggcggta atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    23521 tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg gtattgatgc
    23581 gcccgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca
    23641 ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    23701 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaatgt
    23761 accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc cagataaaaa
    23821 tgccgcccgt gatgtgctgg ggatccctca cgatgcccac tgcctggcgt tgctaccggg
    23881 gagccgtggt gcagaagttg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt
    23941 gcgccagaca tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga
    24001 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    24061 gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta ctggcgtcgg gtacggcagc
    24121 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    24181 cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc
    24241 gggcagagag ttagttaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    24301 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg ataccttccg
    24361 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    24421 gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg tgtggatgaa
    24481 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    24541 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    24601 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    24661 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    24721 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    24781 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    24841 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    24901 ggttttgccc aacacaaagg gtacccaacc gcttttcatc tggaaaaact ggctgaacac
    24961 ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg
    25021 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    25081 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    25141 ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    25201 ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    25261 ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta
    25321 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    25381 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    25441 gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    25501 aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    25561 tttctcgagc tgatccgcac cggcaggccg gacgaagaaa gctatctgca cgcggcggtg
    25621 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca acgacgtgcg ctttatcgac
    25681 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    25741 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg
    25801 tgcgagctgt ttgccgacat ccccgaagct cttgccaaca ccgttgagat cgccaaacgt
    25861 tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    25921 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    25981 ttattccctg acgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    26041 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa
    26101 tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg
    26161 ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    26221 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    26281 tgtatggaga aacgcgatca ggttatcgag cacgtagcgg acatgtacgg tcgtgatgcg
    26341 gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag cggtgatccg cgacgtaggc
    26401 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    26461 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    26521 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    26581 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    26641 ccgctttact gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    26701 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    26761 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    26821 gctgcgatcc cgctggatga taagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    26881 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac
    26941 tgcttcgaag atatgatcgc actggtggca ctgttccgcc ccggcccgtt gcagtcaggg
    27001 atggtggata actttatcga ccgtaaacat ggtcgcgaag agatttccta tccggacgta
    27061 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat catcctgtat
    27121 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    27181 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca gcgttctgta
    27241 tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac
    27301 ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    27361 gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat ggcggcggta
    27421 atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg
    27481 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    27541 gacgacggcg aaatcgtgta tggtattggc gccatcaaag gggtaggtga aggtccgatt
    27601 gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    27661 gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat catgtccggg
    27721 gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg tgatgcgtta
    27781 aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg
    27841 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    27901 caggtggtgt tagatgggga acgtgaaacg ttaggtctgt accttacggg acaccctatc
    27961 aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    28021 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    28081 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    28141 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    28201 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    28261 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    28321 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    28381 gcccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    28441 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    28501 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    28561 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    28621 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    28681 cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct tcgccgatct cggtgcatgg
    28741 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    28801 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    28861 gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga tcattggtca tcaaaaaggt
    28921 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
    28981 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
    29041 gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc tgaagccatt
    29101 gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt
    29161 gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    29221 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
    29281 gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg
    29341 aaactgatcg actccatcat cccggaaccg ctgggcggcg ctcaccgtaa cccggaagcg
    29401 atggcggtat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga tgtgttaagc
    29461 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    29521 gcaaaagttc tgaaaaaggg tcacttcgg
//
LOCUS       DAJTVK010000042        28797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000042 DAJTVK010000000
VERSION     DAJTVK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.042"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO372_001898"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO372_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6341402.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO372_001899"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="OO372_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6341403.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO372_001900"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="OO372_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6341404.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO372_001901"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="OO372_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6341405.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO372_001902"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="OO372_001902"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6341406.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO372_001903"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="OO372_001903"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6341407.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO372_001904"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="OO372_001904"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6341408.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="OO372_001905"
     CDS             complement(4604..5326)
                     /locus_tag="OO372_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6341409.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO372_001906"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="OO372_001906"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6341410.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="OO372_001907"
     CDS             6526..6744
                     /locus_tag="OO372_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6341411.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO372_001908"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="OO372_001908"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6341412.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="OO372_001909"
     CDS             complement(7478..7678)
                     /locus_tag="OO372_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6341413.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO372_001910"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="OO372_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR6341414.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO372_001911"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO372_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6341415.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO372_001912"
     CDS             10175..12088
                     /locus_tag="OO372_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6341416.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO372_001913"
     CDS             12088..13110
                     /locus_tag="OO372_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6341417.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO372_001914"
     CDS             13104..13322
                     /locus_tag="OO372_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6341418.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO372_001915"
     CDS             13376..14245
                     /locus_tag="OO372_001915"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6341419.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="OO372_001916"
     CDS             complement(14300..14704)
                     /locus_tag="OO372_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6341420.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="OO372_001917"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="OO372_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6341421.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="OO372_001918"
     CDS             15689..17779
                     /locus_tag="OO372_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6341422.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO372_001919"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="OO372_001919"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6341423.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO372_001920"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="OO372_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6341424.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="OO372_001921"
     CDS             complement(19747..20349)
                     /locus_tag="OO372_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6341425.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="OO372_001922"
     CDS             complement(20339..20506)
                     /locus_tag="OO372_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6341426.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO372_001923"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="OO372_001923"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6341427.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="OO372_001924"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="OO372_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6341428.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="OO372_001925"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="OO372_001925"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6341429.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="OO372_001926"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="OO372_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6341430.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="OO372_001927"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="OO372_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6341431.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cobA"
                     /locus_tag="OO372_001928"
     CDS             26714..28087
                     /gene="cobA"
                     /locus_tag="OO372_001928"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6341432.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="OO372_001929"
     CDS             28334..28501
                     /locus_tag="OO372_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6341433.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         6995 a         7710 c         7219 g         6873 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctat
//
LOCUS       DAJTVK010000043        28710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000043 DAJTVK010000000
VERSION     DAJTVK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.043"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(301..507)
                     /locus_tag="OO372_001930"
     CDS             complement(301..507)
                     /locus_tag="OO372_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6341434.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            820..909
                     /gene="kdpF"
                     /locus_tag="OO372_001931"
     CDS             820..909
                     /gene="kdpF"
                     /locus_tag="OO372_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6341435.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            909..2582
                     /gene="kdpA"
                     /locus_tag="OO372_001932"
     CDS             909..2582
                     /gene="kdpA"
                     /locus_tag="OO372_001932"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6341436.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2605..4653
                     /gene="kdpB"
                     /locus_tag="OO372_001933"
     CDS             2605..4653
                     /gene="kdpB"
                     /locus_tag="OO372_001933"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR6341437.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4662..5234
                     /gene="kdpC"
                     /locus_tag="OO372_001934"
     CDS             4662..5234
                     /gene="kdpC"
                     /locus_tag="OO372_001934"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6341438.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5227..7911
                     /gene="kdpD"
                     /locus_tag="OO372_001935"
     CDS             5227..7911
                     /gene="kdpD"
                     /locus_tag="OO372_001935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR6341439.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7908..8585
                     /gene="kdpE"
                     /locus_tag="OO372_001936"
     CDS             7908..8585
                     /gene="kdpE"
                     /locus_tag="OO372_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR6341440.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8860..8964
                     /locus_tag="OO372_001937"
     CDS             8860..8964
                     /locus_tag="OO372_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6341441.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9275..11473
                     /gene="speF"
                     /locus_tag="OO372_001938"
     CDS             9275..11473
                     /gene="speF"
                     /locus_tag="OO372_001938"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR6341442.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11470..12789
                     /gene="potE"
                     /locus_tag="OO372_001939"
     CDS             11470..12789
                     /gene="potE"
                     /locus_tag="OO372_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6341443.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OO372_001940"
     CDS             complement(12845..14485)
                     /gene="pgm"
                     /locus_tag="OO372_001940"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6341444.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OO372_001941"
     CDS             complement(14511..15056)
                     /gene="seqA"
                     /locus_tag="OO372_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6341445.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15241..16005
                     /gene="ybfF"
                     /locus_tag="OO372_001942"
     CDS             15241..16005
                     /gene="ybfF"
                     /locus_tag="OO372_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6341446.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16145..16438
                     /gene="ybfE"
                     /locus_tag="OO372_001943"
     CDS             16145..16438
                     /gene="ybfE"
                     /locus_tag="OO372_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6341447.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16578..17108
                     /gene="fldA"
                     /locus_tag="OO372_001944"
     CDS             16578..17108
                     /gene="fldA"
                     /locus_tag="OO372_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6341448.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17318..17404
                     /gene="uof"
                     /locus_tag="OO372_001945"
     CDS             17318..17404
                     /gene="uof"
                     /locus_tag="OO372_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6341449.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17397..17843
                     /gene="fur"
                     /locus_tag="OO372_001946"
     CDS             17397..17843
                     /gene="fur"
                     /locus_tag="OO372_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6341450.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17928..18254)
                     /locus_tag="OO372_001947"
     CDS             complement(17928..18254)
                     /locus_tag="OO372_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341451.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OO372_001948"
                     /pseudo
     CDS             complement(18304..19710)
                     /gene="chiP"
                     /locus_tag="OO372_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
     gene            complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OO372_001949"
     CDS             complement(20288..21952)
                     /gene="glnS"
                     /locus_tag="OO372_001949"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6341452.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OO372_001950"
     CDS             complement(22211..24157)
                     /gene="nagE"
                     /locus_tag="OO372_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6341453.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            24490..25290
                     /gene="nagB"
                     /locus_tag="OO372_001951"
     CDS             24490..25290
                     /gene="nagB"
                     /locus_tag="OO372_001951"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6341454.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25350..26498
                     /gene="nagA"
                     /locus_tag="OO372_001952"
     CDS             25350..26498
                     /gene="nagA"
                     /locus_tag="OO372_001952"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6341455.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            26507..27727
                     /gene="nagC"
                     /locus_tag="OO372_001953"
                     /gene_synonym="nagR"
     CDS             26507..27727
                     /gene="nagC"
                     /locus_tag="OO372_001953"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6341456.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27775..28527
                     /gene="nagD"
                     /locus_tag="OO372_001954"
     CDS             27775..28527
                     /gene="nagD"
                     /locus_tag="OO372_001954"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR6341457.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         6731 a         7316 c         7522 g         7141 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt
      721 tttatacttt ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct
      781 actcttccgg tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgatagcc
      841 ggcgtgttgc tggtgttttt attactgggt tatttggttt atgccctgat caatgcggag
      901 gcgttctgat ggctgcgcaa gggttcttac tgattgccac gtttttactg gtattaatgg
      961 tgctggcgcg tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttccag
     1021 gtacagcggg cgttgaacgt atactctttc gtttacctgg cgtctctgac catgagatga
     1081 actggaagca atatctttgt gccattctcg gcctgaacat gctggggctg gcggtgctgt
     1141 tttttatgtt gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt
     1201 cgtgggatct ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt
     1261 atagcggtga aaccacgttg agctatttca gccagatggc aggcttaacg gtacaaaact
     1321 ttctttctgc cgccagcggg attgcggtga tttttgcctt catccgtgcg tttactcgcc
     1381 agagcatgag cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc
     1441 tagtccctgt ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact
     1501 ttctgcccta tcaggcagtg aataccgttg aaggagcgca acagctgtta cccatgggac
     1561 cagtagcttc tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg
     1621 ccaactcgtc gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg
     1681 cgatcttctt gatcccaaca gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc
     1741 gccaggggcg catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg
     1801 ttatgtgggc agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca
     1861 tcaatatgga aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg
     1921 tgactacggc ggcgtcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg
     1981 gcggcatggt gccgatgtgg ctaatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt
     2041 ctggtcttta cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga
     2101 ttggtcgtac accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg
     2161 cactggcgat tctggttact ccaacactgg tactgatggg cgcggcgttg gcgatgatga
     2221 ccgacgccgg acgtagcgcc atgcttaacc ctggcccgca tggttttagc gaagtgctgt
     2281 acgccgtgtc gtccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact
     2341 ctccgttctg gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta
     2401 tcccggtgat ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg
     2461 gcacgctgcc aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg
     2521 ttggcgcact gacctttatc cctgccctgg cgcttggtcc ggtggcggaa tatctctcct
     2581 gatgatattg agcgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca
     2641 cttgtcgttc aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc
     2701 aatccggtga tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg
     2761 atggcaagcg gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg
     2821 tgggtcaccg tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg
     2881 caggccaaca gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg
     2941 aaatatggcg ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg
     3001 ctggtagaag ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg
     3061 gtcgatgaaa gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat
     3121 tttgcctccg tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc
     3181 gttaaccccg gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga
     3241 cgcaaaacac cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt
     3301 ttactggcaa ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta
     3361 acggtactgg tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca
     3421 gcgatcggcg tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga
     3481 cgtgcagttg aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc
     3541 acactcggta accgtcaggc gtcggagttt atccccgcgc agggcgtgga tgaaaaaacg
     3601 ctggctgacg ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg tcgcagtatt
     3661 gtgatcctcg ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc
     3721 acctttgtac cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg
     3781 atccgtaaag gttctgtcga tgccattcgc cgccatattg aggctaacgg cggtcacttc
     3841 cctgccgatg ttgatcaaaa agtcgatcag gttgcgcgtc agggagccac gccgctggtg
     3901 gtggtggaag gttctcgtgt gctgggcgtt attgcgctga aagatatcgt caaaggcggt
     3961 attaaagagc gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc
     4021 gataaccgtc tgactgccgc cgcgatagct gcggaagcgg gtgtcgatga ttttctcgcc
     4081 gaagcaacac cggaggccaa gctggcattg atccgtcagt atcaggcgga aggtcgtttg
     4141 gtagcgatga ccggcgacgg caccaacgat gctccggcgc tggcgcaggc agatgtcgcg
     4201 gtggcgatga actccggcac ccaggcggcg aaagaggcgg gcaatatggt ggatctcgac
     4261 tctaacccga ccaagttgat cgaggtggtg cacattggca aacagatgct gatgacccgt
     4321 ggctcgctga ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg
     4381 gcggcgttcg cggcaacata tccgcagtta aatgcgctga acatcatgcg cctgcattcg
     4441 cccgactccg ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt
     4501 cctctggcgt taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt
     4561 aacttatgga tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt
     4621 gatttactgc tgaccgtttg cggtctggtg tgaggtttac catgagtgga ttacgtccgg
     4681 cattatcgac atttctcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca
     4741 ccgcactggg gcaatggtgg tttccctggc aggccaacgg ttcgttgatc cgtgaaggtg
     4801 atacggtgcg tggctcggca ttaatcgggc agaattttac cggcaacggc tattttcatg
     4861 gtcgcccgtc agcaacggct gaaatgccct ataatccaca ggcttctggc gggagcaatc
     4921 tggcggtcag taaccctgag ctggataaac taatagccgc acgcgttgct gcattacggg
     4981 ccgctaaccc ggatgccagc gcgagcattc cggttgaact ggtgacggca tcggcaagcg
     5041 ggctggacaa taatatcacc ccgcaagcgg cggcctggca gatcccacgc atcgcgaaag
     5101 cgcgtaatct cagcgttgaa cagctcacgc agctgatcgc aaaatacagc caacaaccgc
     5161 tggtgaaata tatcggccag ccggtcgtca acattgtcga actcaatctg gcgctggata
     5221 agcttgatga ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc
     5281 gcgccgcatc gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc
     5341 tgggcgatgc tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc
     5401 gtggtagaaa cccacgggcg aaaagatacc gccgcaatgc tggaagggct ggctgttctg
     5461 ccgccaaaac gcctggcgta ccgtgggcgg cacatctgcg agtttgacct cgatgccgcc
     5521 ctcgcccgcc gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt
     5581 tctcgtcacc ctaaacgctg gcaggacatc gaagagctgc tggaagcagg cattgatgtt
     5641 ttcactaccg tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc
     5701 ggaattcagg tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtggtt
     5761 ctggtggact tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt
     5821 gccgggcagg cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg
     5881 cgcgaactgg cactgcgccg tactgccgat cgcgttgatg agcaaatgcg cgcctggcgg
     5941 gggcatccta gcgaagagaa agtctggcac acgcgcgacg cgatcctttt atgcatcggc
     6001 cataacaccg gtagtgagaa gctggtccgc gcggcggcgc ggctggcgtc acggctgggt
     6061 agcgtctggc acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa
     6121 cgtcgtgcaa ttctcagcgc cttacgtctg gcgcaggaac tgggcgcgga gacggcaaca
     6181 ctttctgatc cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc
     6241 aagattattc tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac
     6301 cgactggcgc gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc
     6361 gcccgcacga ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag
     6421 attcagggat gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag
     6481 tggctgatgg cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg
     6541 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6601 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6661 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggcgtg
     6721 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6781 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6841 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6901 cagccgttaa cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt
     6961 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     7021 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     7081 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacgtttac gctgttagtc
     7141 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7201 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7261 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7321 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7381 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7441 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg
     7501 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7561 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7621 attggtatcg atgcccactt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7681 cccggtcttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7741 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7801 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7861 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7921 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7981 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     8041 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     8101 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     8161 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca
     8221 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc
     8281 ccgatccgct ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc
     8341 ggggtgagga agaggtgcat ctcacaccaa ttgagttccg cctgctggcg gtgctgctca
     8401 acaatgccgg aaaagtactc acccagcgcc agctccttaa ccaggtgtgg gggccaaacg
     8461 cggtcgaaca cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac
     8521 aggatccggc ccggcctcgc cacttcatta ctgaaaccgg tattggatat cggtttatgc
     8581 tttgaattgt tattcaataa attcgcttaa atagtgatta atttattatt aaacctgcgc
     8641 cgaatattat tatttggcag gtttaatttc ttaacgcaaa tataaaacac gaaattacaa
     8701 catttaagta acaaaacagc atctaatatg gcgatattca taattaatga tttattatct
     8761 ggggtatgat cgttttttgt tgatcttctt cacagattat agccatttca tggatagaat
     8821 aactctacct tcaactgaca cagcaagagg taaaggtaaa tggaaaataa taaccgctta
     8881 atgcctcata taaggcggac aacccatatc atgaagtttg cccatcgtaa tagttttgac
     8941 tttcatttct ttaatgcccg ctagtcttct gactaaaggg caccccaacg tacaggtctc
     9001 cctgacttta agcattacag gttaatacct gtattcctcg gtgctcatat actgctaacc
     9061 cttttaaact ctaaataatt cgagtcgcag cacttgcaac ttgaggtatg acgagtatag
     9121 ccagttaccg ggctggtctg ggttattgca tctgcaaaaa gcaaactact gatttattta
     9181 tcagcggtgg agctttgctt tttttccggc gtgatcgatt tctcctttga gaaattgagg
     9241 acctgctatt acctgaaata aagagatgaa taaaatgtca gaattaaaaa ttgccgttag
     9301 ccgttcttgc ccggattgtt tttccactca tcgtgcatgc gtgaatattg acgaaagtaa
     9361 ttatattgac gttgccgcca ttattttatc agtcaatgat gttgaacgtg gaaaactcga
     9421 tgaaatagac gctactggct atggcattcc tgtttttatt gcaacggaaa atgaagaacg
     9481 tgtaccagca gaatatctgc cacgtatttc tggtgtcttc gagcattgcg aatcacggaa
     9541 agagttttat ggacgtcagt tagaaaccgc tgctagccat tatgaaacgc aactgcgccc
     9601 gcccttcttc cgcgcactgg tcgattatgt caatcagggc aatagcgctt ttgactgccc
     9661 gggtcatcag ggaggtgagt ttttccgtcg tcatccggca gggaatcagt tcgtggagta
     9721 cttcggagaa acgctgttcc gttccgactt atgcaacgca gacgtagcaa tgggcgatct
     9781 gctgattcac gaaggcgcgc catgcattgc acagcaacat gcggcaaaag tgtttaatgc
     9841 cgataaaaca tacttcgttc taaatggcac ttcatcttct aacaaagtgg ttttaaacgc
     9901 cctgctaaca ccgggtgatc tggtgctgtt tgatcgcaat aaccacaaat ctaaccacca
     9961 cggagcgttg ctacaggctg gtgcaacacc ggtttatctg gaaacggcac gtaacccgta
    10021 tggctttatc ggtggcattg atgcgcactg ttttgaagaa agttacctgc gtgagctgat
    10081 cgcggaagtc gcaccgcagc gggcaaaaga ggctcgtcct ttccgcctcg ctgtgattca
    10141 gttaggcacc tacgacggta cgatttataa cgcccgccag gtggtggata aaattggtca
    10201 tctgtgtgac tacattctgt ttgactccgc atgggttggc tatgagcaat ttatcccgat
    10261 gatggctgac tgttcgccgc tgttgctgga tcttaatgag aacgatccgg gtattctggt
    10321 tacgcaatcc gtacataaac agcaggctgg tttttctcaa acttcacaaa tccataaaaa
    10381 agacagccac atcaaagggc aacagcgtta tgtaccgcac aaacgcatga acaacgcctt
    10441 tatgatgcac gcctccacca gcccgttcta tccgctgttt gccgcactgg atatcaacgc
    10501 caaaatgcat gaaggtgtca gcggtcgtaa tatgtggatg gattgtgtgg taaatggcat
    10561 taatgcccgc aaactgatcc tcgataactg tcagcatatt cgtccgttcg tacctgaact
    10621 ggtggatggt aaaccctggc agtcgtatga aacagcgcaa attgcggttg atctgcgctt
    10681 cttccagttt gtaccagggg aacactggca ttcttttgaa ggctatgcag agaatcaata
    10741 ctttgtcgat ccttgcaaac tgttgctgac aaccccaggt attgatgcac gtaacggcga
    10801 atatgaagcg ttcggtgtac ccgcgacgat tcttgctaac ttcctgcgcg aaaatggcgt
    10861 agtgccggaa aaatgcgatc ttaactccat cctcttcctg ctgactccgg cagaagatat
    10921 ggccaaactt cagcaacttg ttgccctgct ggtacgcttc gaaaaactgc ttgagtccga
    10981 cgcgccatta gcagaagtgc tgccttccat ctacaaacag catgaagagc gctacgccgg
    11041 ttataccctg cgtcagttgt gccaggaaat gcatgatttg tatgcccgcc acaacgtgaa
    11101 acaactgcaa aaagagatgt tccgcaaagc gcacttccca cacgtcagca tgaatccgca
    11161 agaagccaac tacgcctatt tacgcggtga agtggagctg gttcgtctgc cggatgccga
    11221 aggtcgtatc gctgccgaag gggcgcttcc ttatcctccg ggtgtgctgt gtgttgttcc
    11281 gggtgaaatc tggggtggtg ctgttttgcg ttacttcagc gctctggaag aagggatcaa
    11341 cctgctgcca ggttttgcac cggagctgca gggtgtctat atcgaagaac atgatggtcg
    11401 taagcaagtt tggtgctatg tcatcaagcc tcgtgatgcg caaagcaccc tgttgaaagg
    11461 ggaaaaatta tgagtcaggc taaatcgaac aaaatgggcg tcgttcagtt aaccatactg
    11521 acgatggtca acatgatggg ctccggtatc atcatgctgc cgacaaagct tgccgaagtt
    11581 gggacaattt caattatctc ctggctggtg acagccgtag gctcaatggc actggcatgg
    11641 gcattcgcaa aatgcggtat gttcagccgt aaatcaggtg gtatgggcgg ttatgccgag
    11701 tatgctttcg gtaaatccgg taactttatg gcgaactata cctacggcgt ctcactgctg
    11761 attgctaacg tcgcgattgc tatttcggcg gttggttacg gcaccgaact gctcggcgcg
    11821 agtttgtcgc cagtgcagat tggtcttgcg accatcgggg tgctgtggat ttgtaccgtg
    11881 gctaactttg gtggtgcgcg cattaccggg caaatcagta gcattaccgt gtggggggtc
    11941 attattccgg tggtaggcct gtgcattatc ggctggttct ggtttagccc gacgctttac
    12001 gttgattcct ggaatccgca tcatgcaccg ttcttcagtg cagtaggttc ttccatcgct
    12061 atgacgctgt gggcttttct cggtctggag tctgcgtgtg cgaatactga tgtagtggaa
    12121 aacccggaac gtaatgtgcc aatcgcggta ctcggcggta cgttaggtgc ggcggtgatt
    12181 tatatcgtct ccaccaacgt gattgcaggg attgtgccta atatggagct ggcaaattca
    12241 acggcaccat ttggcctggc cttcgcacag atgttcacac cagaagtggg taaagtcatt
    12301 atggcgctga tggtgatgtc ctgctgcggt tcgctgcttg gctggcagtt caccattgcc
    12361 caggtgttta agtcttcatc tgatgaaggc tacttcccta aaattttctc ccgtgtaacc
    12421 aaagtggatg caccggtgca gggaatgttg accattgtga ttatccagag cgggttggcg
    12481 ctgatgacga ttagcccgtc gctaaacagt cagttcaacg tgctggttaa cctggccgtg
    12541 gtgaccaata tcattccgta tattctgtca atggcggcat tagtcattat tcagaaggtc
    12601 gctaatgtgc ccccctcaaa agcgaaagtt gcaaactttg ttgcttttgt tggcgcgatg
    12661 tatagctttt atgcgctgta ctcatccggg gaagaagcca tgctgtacgg ttccatcgtg
    12721 accttccttg gttggacact gtatggtctg gtatcaccac gctttgaact gaaaaataaa
    12781 cacggttaaa aaagcaaagt aaaaaagggc gatcttgcga ccgccctttt tttattaaat
    12841 gtgtttacgc gtttttcaga acttcgctaa caatctcaac cgcttctttc tcaatctgct
    12901 tgcgatgttc ttcaccgagg aagctttcgc agtagatctt atatgcgtct tccgtgcctg
    12961 acggacgcgc ggcgaaccag ccgttgtcag tcatcacttt cagaccgccg atagaagcac
    13021 cattgcccgg tgccgccgtc aggcgcgcgg tgatcgggtc gcctgccagg gtgctggcgc
    13081 tcaccatttc cggagacagc ttagacagcg ccgctttttg tgcggaagtc gcagctgcct
    13141 gcaaacggtt gtagctcggc gcaccaaagc gttttgccag ttcgttgtag tgttcctgcg
    13201 ggttcttacc ggtgacagcg gtgatttccg ccgccagcag acacatgatg atgccgtctt
    13261 tgtcggtgga ccacggcgtg ccgtcgaaac gcaggaagga agcccctgcg ctctcttcgc
    13321 cgccaaagcc gaagctgccg tcgaacagac catcaacaaa ccatttgaaa cctaccggga
    13381 cttctaccag cttgcggccc aagtcattga ccacacggtc gatcatcgca gaagaaacca
    13441 gcgttttacc gacagcaaca tctttgcccc actgcggacg atgctggaac aggtagttga
    13501 tcgccaccgc caggtagtgg ttcggattca tcaaacctgc cggagtgacg ataccgtgac
    13561 ggtcataatc cgggtcgtta gcaaacgcca gatcgaactt atcacgcaat gccagcaggc
    13621 ccgccatcgc acactcggag gagcagtcca tacggatcgc gccgtcttta tcgaggtgca
    13681 taaagcggaa ggtttgatcg acctgatcgt taacgatggt caggttgagg ttgtaatact
    13741 cgccaattcg cttccagtat tcgataccgg aaccacccag cggatcaacg cccagcgtca
    13801 ggcccgtttt ctgaatcgcc gccatatcaa cgatatcggc cagaccttcc acaaactgct
    13861 gcaccagatc ctgctctttc acatgaccgg atgccatcgc ttcgtcgagg gagatacgct
    13921 tcacgccttt caggccatcg gccatcagtg cgttggcacg atcttccacc actttagtga
    13981 cgttggtatc agccgggcca ccatttggcg gattgtactt gataccacca tcttccggcg
    14041 ggttatggga gggtgtaatc acgataccgt ctgccagcgg gccacctttt ttattatgaa
    14101 ccaggatggc attggaaacg gcaggcgtcg gagtgaagcc attgttttcc tgcacaatga
    14161 catcaacgcc gttcgctgcc agcacttcca gtacggaaat gaacgcaggc tcggacaggg
    14221 cgtgagtatc tttacccaca tagcaagggc cagtgatgcc gtttttcgca cgttcttcag
    14281 caattgcctg agcgatcgcc agaatgtgcg gctcgttaaa gctgtggcgc gctgcactgc
    14341 cacggtgacc ggaagtaccg aatttcaccg cgtgctccgc attccctgct tctggtttca
    14401 gtacataata ttgcgccgtc agttgggcga cgttaatcaa atcactctgt tgtgcaggtt
    14461 ggcctgcacg attgtggatt gccattgctt tgtcctttgt ctgcaacgtt ttagatagtt
    14521 ccgcaaacct tctcaatcaa ttccgccggg aattgcatcg actgcatgat gtgttcgatc
    14581 atgctgcatt tacggccggt gttggtgttg gtgatcaccc aatacggcgt gcctggcaca
    14641 tgtttcggct tggtctgatt accatttttc agcagcgttt gttcatctgc cgcaaagtaa
    14701 acgcgtgtac gaccgtgcaa cgattccgtt gcttcggcaa acgcctgggc gtcaagagaa
    14761 tatagtgtag acaacagcag cataaagcga ttgaccgctc gcttttgctc tgcgtattcg
    14821 tcagaaagca gaagttcacg cattgcgcga accttgtctt taatcgtttt gaccggcttc
    14881 gcttcgacga tagcaggtga cgcaacgcga acctctttcg tcaccggagc agcaggctgt
    14941 gatgcggcgg aaaatttcaa catacgccgt aaaatgtcgg atgcgctctc gccgatatgc
    15001 ttagtgtggc tggcaatata gctgtagagt tcatcatcaa cttcaatcgt tttcatctta
    15061 atccagtaca gtgtcttagc tgaatacaag tcgccaagag tatatgaata aatcccggca
    15121 gcggatagcg tcaagcctgg cttgcagaaa aactcggaat aaaccttatt tgttgcagcc
    15181 ctgtggcaca atggtcaaca taatacccta acccgacaac gcgaaaaaaa gaactttgcc
    15241 atgaaattga atatccgcgc gcaaactgca caaaaccagc acaataattc tcccatcgtt
    15301 cttgtccatg gtctgtttgg tagcctcgac aaccttggcg tactggctcg cgatctggta
    15361 aacgatcaca atatcatcca ggttgatatg cgtaaccacg gtctttcacc gagagatccg
    15421 gtaatgaatt acccggcgat ggcgcaggat cttgttgata ctctggatgc acagcagatc
    15481 gacaaagcaa catttatcgg tcactccatg ggcggtaaag cggtaatggc acttacggca
    15541 ctggcccgcg atcgcatcga taaactggtg gcgatcgata tcgcaccggt cgactatcac
    15601 gtacgccgtc atgatgagat tttcgcggct atcaacgcgg tcagtgaatc ggacgcacaa
    15661 actcgccagc aagcagcagc aataatgcac cagcatctta atgaagaagg ggtgattcag
    15721 tttctgttga aatcttttgt tgacggggag tggcgcttta acgtgccggt attgtgggat
    15781 cagtatccgc atattgtggg ttgggagaaa atcccggcat gggatcaccc tgccctgttt
    15841 atccctggcg gcaattctcc gtatgttagc gagcagtacc gtgatgattt actggcacaa
    15901 tttccacagg cacgggcgca tgtgattgca ggcgcgggtc actgggtcca tgctgaaaaa
    15961 ccggatgcgg tattacgcgc tatccgtcgc tatctcaatg attaacgcaa ctgattaaaa
    16021 acccagcgtc cgcgtcgctt tccgcgcgtg ggcgttggcg tgactcatcg cgctgatgta
    16081 ttatggcgcg ctatccatcc gggcagaggc ctggctgatt gtttcccccg aagtcaccaa
    16141 gatcatggcc aaagaacaaa cggaccgtac gacattagat ctgttcgcgc acgagcgtcg
    16201 accgggacga ccgaaaacta atccgctttc gcgcgatgaa cagctgcgta ttaataaacg
    16261 caaccagcta aaacgcgaca aagtacgtgg ccttaagcgt gtcgaactga agctgaacgc
    16321 ggaagctgtc gaggcgctga acgagctggc ggagtcgcgc aatatgagcc gtagcgaact
    16381 gatcgaagag atgttaatgc agcaactggc ggcgctgcgt agtcagggca tcgtttaaat
    16441 ttccactttc atgtagcaca gtgtgcagtc ctgctcgttt gctgattccg caagactgcc
    16501 tgttctgcta tgattgcctt tatccgtggg caattttcca cccccatttc aataagtttc
    16561 aagaggttat ttcactcatg gctatcactg gcatcttttt cggcagcgac accggtaata
    16621 ccgaaaatat cgcaaaaatg attcaaaaac agcttggtaa agacgttgcc gatgtccatg
    16681 acattgcaaa aagcagcaaa gaagatctgg aagcttatga cattctgctg ctgggcatcc
    16741 caacctggta ttatggcgaa gcgcagtgtg actgggatga cttcttcccg actctcgaag
    16801 agattgattt caacggcaaa ctggttgcgc tgtttggttg tggtgaccag gaagattacg
    16861 ccgaatattt ctgcgacgca ttgggcacca tccgcgacat cattgaaccg cgcggtgcaa
    16921 cgatcgttgg tcactggcca actgcgggct atcatttcga agcatcaaaa ggtctggctg
    16981 atgacgacca ctttgtcggt ctggctatcg acgaagaccg tcagccggaa ctgaccgctg
    17041 aacgtgtaga aaaatgggtt aaacagattt ctgaagagtt gcatctcgac gaaattctca
    17101 atgcctgatg tgatgcggcg tagactcatg tctacgccgt attaatagat aatgccaatc
    17161 aaaataattg ctacaaattt gtaacttttg ctgttgtacc tgtacaatgt cccggtgttc
    17221 aagtggcctt gccgttgtaa atgtaagctg tgccacgttt ttattaacaa tatttgccag
    17281 ggacttgtgg ttttcattta ggcgtggcaa ttctataatg atacgcatta tctcaagagc
    17341 aaattctgtc acttcttcta atgaagtgaa ccgcttagta acaggacaga ttccgcatga
    17401 ctgataacaa taccgcccta aagaaagctg gcctgaaagt aacgcttcct cgtttaaaaa
    17461 tcctagaagt tcttcaggaa ccggacaacc atcacgtcag tgcggaagat ttatacaaac
    17521 gtctgatcga tatgggtgaa gaaattggtc tggctacggt atatcgcgta ctgaaccagt
    17581 ttgacgacgc tggtatcgtc acccgccaca attttgaagg cggtaaatcc gtatttgaac
    17641 tgacacagca acatcaccac gatcacctga tctgcctcga ctgcggcaag gttatcgaat
    17701 ttagtgatga ttccatcgaa gcgcgtcagc gtgaaattgc cgcaaaacat ggcattcgcc
    17761 tgactaacca cagtctctat ctttacggtc actgtgccga aggcgattgc cgcgaagatg
    17821 aacatgcgca cgaaggcaaa taagccagcc tgaatgagaa aaagccaacc tgcgggttgg
    17881 cttttttatg caagggaaat aagaaaagcg agagttacag ctctcactta tttgttatta
    17941 ctgcgaattt cctgccaaac tttatcacaa tcggctttca ccgcctgatc atttccggtt
    18001 tgcgttgcac gcagatactg ctgataatcc agtacgcgga cactttcctg gtcggcaaaa
    18061 tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc ttcaattttt
    18121 tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag acgactgtct
    18181 tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat ggcgatgaga
    18241 atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct gccgcgtcgg
    18301 gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct catcctggaa
    18361 gatgttgccg taaccaccgc cccagcttgg gatgtcggag tggttgtcgt attcggtgaa
    18421 gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga ccgcatccag
    18481 gctgtatgca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg gattgctctg
    18541 ctaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg
    18601 aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt cgccgttagc
    18661 gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg aagcgtaggt
    18721 tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc aggtgccttc
    18781 gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc
    18841 ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg taccatagaa
    18901 ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa
    18961 atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga agttattttt
    19021 gaagtcgtat ttcgccccaa gggagtgcag ataatcaact ttggtggttt tatcgttctg
    19081 ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc acatgtagga
    19141 gaaactcaac gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg ccccctgata
    19201 agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg gctgaatgta
    19261 acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat acaggcttat
    19321 accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg aaaacgcgat
    19381 ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa acgcagcaat
    19441 atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg cattccaggt
    19501 ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac ggtcgcgttc
    19561 acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg
    19621 agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag ccagcgccag
    19681 cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt ataaattgct
    19741 gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    19801 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    19861 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    19921 gcggttgaaa ggcttgcaga agaataaaag cgtgaaattc cagcaagatg aaagcggtta
    19981 cattttattt aattatgttt tgttatttta aatttatttt atggataaat tgcgaagcgg
    20041 aattaattcg cgaagaaaag cagaaaaaac gccgctgaac gcggcgtttt ggattgtttg
    20101 catatttctt gccggatgcg gcgtgaacgc cttaaccggc ctacaaaagc acgcaaactc
    20161 aatatattgc agagatcatg taggcctgat aagcgtagtg catcaggcaa tttagcgttt
    20221 gaaactgttt gtctgttcct tgccggatgc gacgtaaacg ccccatccgg catagcgaaa
    20281 cttaaaatta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg cggttaaata
    20341 ccggtttttc cgccgtagaa tggcggctat cgaggcagaa gtaaccttca cgctcaaact
    20401 ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc tgtttgatca
    20461 ccagcgattc cgggttaatc accgacagga aatcatccgc agcaccaggg ttcggcacgc
    20521 tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc atgtgccgcg ctcacccagt
    20581 gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg tcggcgtcat
    20641 aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt tctgccttaa
    20701 tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg tactgcttgt
    20761 tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaac ggcacctggc
    20821 ggctacccat ttccggtttg ttcggatggt tcggcatggt aaccatttcg ccttcgccct
    20881 gatagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc ggggcgtttt
    20941 cgttgagatc ttcacggatg caggattcca gcgacgccat ctcaatggtg ttgtcctgct
    21001 tggtcacgcc gatgcgtttg cagaactcac gaatagaagc agcagtgtaa ccacgacgac
    21061 gcagaccgga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc ttgtcggtca
    21121 ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg cgcgagaatt
    21181 cgtactggcg cgggtgaaca ggaatggtga tgttgtccag cacccagtcg tacaggcgac
    21241 ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagg gcatcgctga
    21301 tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt gttgccagtc tggtggtgtt
    21361 cagcaaattt aatgcggtac agcaccggat cgcgcatcac gataaacggt gaagccatgt
    21421 cgattttcgc acgcaggcag gctttacctt cttcaaaacc accggcacgc attttttcga
    21481 acagcgccag gttctcttca acgctgcggt cgcggtacgg gctgttttta cccggttgcg
    21541 tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg tacgccaggc
    21601 ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg gaggagtaac
    21661 ggacgttacc agaccagtga aaacctaacc actctacgtc gtttttgatc gactcaacat
    21721 actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac tggcctttat
    21781 agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata tgcagatagc
    21841 cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg gccagatctt
    21901 catcgatgat ctgacggata aagttagtcg ggcgggcttc tgcctcactc atcgtggatt
    21961 cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca ggctgacaac
    22021 tgttagttag cgaaaatcga aaatctcact gagattatgg ggatgattga tgaaaaaaaa
    22081 agcggcagag aagatctcca ccgcttttgc cagtcccgcg tcattgttgg atgcgcgtct
    22141 tatccaacca ggactgagct tttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
    22201 ataaagcaga ttactttttg atttcataca gcggtgtttg acccgccaca acattgccct
    22261 gagctttaat gatcaagcca ctgaaatcgt cgatattgct gcaaaccacc gggctaatca
    22321 tcgaacgggc gttagcgttc aggtaatcca gatccatttc cagaatcggt tgccctgcgc
    22381 tcacctgcgc gccctcttcc accagacgtt taaagccttt accttccagc gctacggtgt
    22441 cgatacccat gtggacgaca atctccgcgc ctttttcggt ttctaggcag aacgcgtggt
    22501 tggtgttgaa gattttcacg attgtccctg cggctggtga tacgacgatt ttatctgtcg
    22561 gtttcaccgc cacaccgtca cccaccgctt tgctggcgaa tgcttcgtca gaaacctgat
    22621 ccagtgccac gacatcaccg gtaatcggcg ataccagctc cgcgatagat accgcgtttg
    22681 gtacagcctg cggttttgct acaggagcgg cagttgccgg agttgcttca gctgacgcag
    22741 ctgctaccgg gccacgggca acgactttct tcatcgcatc gccaatggat tctgctttcg
    22801 cgccaacaat cacctgaatg gtctgtttgt tcagttttac tacaccagaa gcacccagac
    22861 gtttacacat cgcatcgttg acgcgagctg agtcagccac ggtaaggcgc agacgggtaa
    22921 tacaggcgtc aatcgctttc aggttgtcag taccgccaac cgcagcaata tagttggttg
    22981 ccagttgagt cagaccttct tcagtattgc tgttagcctc ttcagttacg atctcgtctt
    23041 ctttatcttc acgacccggc gttttcaggt tgaacatgcg gataaccaaa ctgaacacca
    23101 cgaagtagat agcgaagaag acaacaccca tcaccagcag catccagacg ttctggctgg
    23161 cggccggcag gttatacatc aacgcgtagt cgatagcccc cgcagagaaa gagaagccag
    23221 catggatacc cagcagcgtt gccacaaaca ggctgatacc ggtcagcagt gcgtgcagga
    23281 ggtacagcag cggagcaagg aacatgaaca ggaattccag cggctcagtc acaccggtca
    23341 ggaacgcagt aacagcaaca gaaagcagca tcccgccaac catcggacga cgctctttcg
    23401 gtgctgcgaa gtacatcgcc agcgccgcac ccggcagacc gaacatcatg atcgggaaga
    23461 agccggacat gaacatcccc gcggtgccgt caccagcgta gaaacggttg atgtcgccgt
    23521 ggaaaaccgt acccgccgcg ttggtgaatt caccaatctg gaaccaggcg atggtgttca
    23581 gcacctgatg cagaccggtt gggatcagca gacggttgat gaaaccaaag ataccggaac
    23641 ccagcgcgcc tgcagaaacg atccactcgc cgcctgcatg gatagcgtgc tgtactggcg
    23701 gccagacgta accaaaaatg gccgccagca ccaggcagaa aaagccggtg gcaatcggca
    23761 caaagcgttt gccgccgaag aagctcagga agtccggcag tttaatatcg gaccaacggt
    23821 tataggctgc gccaccaacc agaccggtaa tgatacccgc cagtacaccc atgttaattt
    23881 ctgggttgat ggtcaccatc gctttggtta acacaaagta acctaccgca cccgccagcg
    23941 ccgccgcacc tgcgctgtct ttcgaccagc tggatgccac accgatggcg aagattaatg
    24001 cgaggttatc aaaaatcgca ccgcccgcct gggcaataaa cgcaacgtta agtaaatctg
    24061 gctgaccgaa tcgcagcaac agtgccgcca ccggcagcac cgcgataggg agctgtaacg
    24121 ccctaccgag tcgctggaaa aaacctaaaa tattcatctt attcccccta cgagaaccct
    24181 atttggctcg tttaaagccg tatttttatt ttgctgcaaa ttgtactgcc gatgttctgt
    24241 aatcagattg ttagatcatc tgctacagag tgtgtgaaaa tttaattcgt atcgcaaatt
    24301 aaacgcgtgc cttttgtgag ttttgtcacc aaatatcgtt atcatcactc ccttttactg
    24361 gctaaagcag aaaacttatt ttatcattca aaaaatcagg tcggattgac gcctgtctgc
    24421 gcaaatccag gttacgctta aagatgccta atccgccaac ggcttacatt ttacttattg
    24481 aggtgaataa tgagactgat ccccctaact accgctgaac aggtcggcaa atgggctgct
    24541 cgccatatcg tcaatcgtat caatgcgttc aaaccgactg ccgatcgtcc gtttgttctg
    24601 ggcctgccga ctggcggcac gccgatgacc acctataaag cgttagtcga aatgcataaa
    24661 gcaggccagg tcagctttaa gcacgttgtc accttcaaca tggacgaata tgtcggtctg
    24721 ccgaaagagc atccggaaag ctactacagc tttatgcacc gtaatttctt cgatcacgtt
    24781 gatattccag cagaaaacat caaccttctc aacggcaacg ccccggatat cgacgccgaa
    24841 tgccgccagt atgaagaaaa aatccgttct tacggaaaaa ttcatctgtt tatgggcggt
    24901 gtaggtaacg acggtcatat tgcatttaac gaaccggcgt cttctctggc ttctcgtact
    24961 cgtatcaaaa ccctgactca tgacactcgc gtcgcgaact ctcgtttctt tgataacgat
    25021 gtcaatcagg tgccaaaata tgccctgact gtcggtgttg gtacattgct ggatgccgaa
    25081 gaagtgatga ttctggtgct gggtagccag aaagcactgg cactgcaggc tgcagttgaa
    25141 ggttgcgtga accacatgtg gaccatcagc tgtctgcaac tgcatccaaa agcgatcatg
    25201 gtgtgcgatg aaccttccac catggagctg aaagttaaga ctttaagata tttcaatgaa
    25261 ttagaagcag aaaatatcaa aggtctgtaa ttgttatccc tgccctggct ccttgctcag
    25321 ggcaatattt tttaaaatcg gggggcagaa tgtatgcatt aacccagggc cggatcttta
    25381 ccggccacga atttcttgat gaccacgcgg ttgttatcgc tgatggcctg attaaaagcg
    25441 tctgtccggt agcggaactg ccgccagaga tcgaacaacg ttcactgaac ggggccattc
    25501 tctcccccgg ttttatcgat gtgcagttaa acggctgcgg cggcgtacag tttaacgaca
    25561 ccgctgaagc ggtcagcgtg gaaacgctgg aaatcatgca gaaagccaat gagaaatcag
    25621 gctgtactaa ctatctgccg acgcttatca ccaccagcga tgagctgatg aaacagggcg
    25681 tgcgcgttat gcgcgagtac ctggcaagac atccgaatca ggcgttaggt ctgcatctgg
    25741 aaggtccgtg gctgaatctg gtaaaaaaag gcacccataa tccgaatttt gtgcgtaagc
    25801 ctgatgccgc gctggtcgat ttcctgtgtg aaaacgccga cgtcattacc aaagtgaccc
    25861 tggcaccgga aatggttcct gcggaagtca tcagcaaact ggcaaatgcc gggattgtgg
    25921 tttctgccgg tcactccaac gcgacgttga aagaagcgaa agccggtttc cgcgcgggga
    25981 ttacctttgc cacccatctg tacaacgcga tgccgtatat taccggtcgt gaaccgggcc
    26041 tggcgggcgc gatcctcgac gaagctgaca tttattgcgg tattatcgct gatggcctgc
    26101 atgttgatta cgccaacatt cgtaacgcta aacgccttaa aggcgacaaa ctgtgtctgg
    26161 ttaccgacgc caccgcgcca gcaggtgcca acattgaaca gttcattttt gcgggtaaaa
    26221 caatatacta ccgtaacgga ctttgtgtgg atgagaacgg tacgttaagc ggttcatcct
    26281 taaccatgat tgaaggcgtg cgtaatctgg tcgaacattg cggtatcgca ctggatgaag
    26341 tgctgcgtat ggcgacgctc tatccggcgc gtgcgattgg cgttgagaaa cgtctcggca
    26401 cactcgccgc aggtaaagta gccaacctga cctcattcac acctgatttt aaaatcacca
    26461 agaccatcgt taacggtaac gaggtcgtaa ctcaataaga gaaagtatga caccaggcgg
    26521 acaagctcag ataggtaatg ttgatctcgt aaaacagctt aacagcgcgg cggtttatcg
    26581 cctgattgac cagtacgggc caatctcgcg gattcagatt gccgagcaaa gccagcttgc
    26641 ccccgccagc gtaaccaaaa ttacgcgtca gcttatcgaa cgcgggctga tcaaagaagt
    26701 tgatcagcag gcctccaccg ggggccgccg cgctatctcc atcgtcaccg aaacccgcaa
    26761 tttccacgca atcggcgtac ggcttggtcg tcacgacgcc accatcaccc tgtttgatct
    26821 cagcagcaaa gtgctggcag aagaacatta cccgctgccg gaacgtaccc agcaaacgct
    26881 ggaacatgcc ctgttgaatg ccattgctca gtttattgat agctaccagc gcaaactgcg
    26941 cgagctgatc gcgatttcgg tgatcctgcc agggcttgtt gacccggaca gcggcaaaat
    27001 tcattacatg ccgcatattc aggtagaaaa ctgggggctg gtagaagctc tggaagaacg
    27061 ttttaaagtg acctgtttcg ttggtcacga tatccgtagt ctggcgctgg cggagcacta
    27121 cttcggtgca agtcaggatt gcgaagactc cattctggtg cgtgtccatc gcggaaccgg
    27181 ggccgggatt atctctaacg ggcgcatttt tattggccgc aacggtaacg tcggtgaaat
    27241 tggccatatt caggtcgaac cgctgggtga acgctgccac tgcggcaact ttggctgcct
    27301 ggaaactatc gccgccaacg ctgccattga acaacgggtg ttgaatctgt taaagcaggg
    27361 ctaccagagc cgcgtgccgc tggacgactg caccatcaaa actatctgca aagccgcgaa
    27421 caaaggcgat agtctggcgt cggaagtaat tgagtatgtc ggtcgtcatc tgggtaaaac
    27481 catcgccatt gctatcaact tatttaatcc gcaaaaaatt gttattgccg gtgaaatcac
    27541 cgaagccgat aaagtgctgc tccctgctat tgaaagctgc attaataccc aggcgctgaa
    27601 ggcgtttcgt actaatctgc cggtggtacg ttctgagctg gatcaccgct cggcaatcgg
    27661 cgcttttgcg ctggtaaaac gcgccatgct caacggtatt ttgctccagc atttgctgga
    27721 aaattaatgt gcttttatag tggcgcttat tgttgtcaat attctgggta gtccatgacc
    27781 attaaaaatg taatttgcga tatcgacggc gtgctgatgc acgataacgt cgccgtaccg
    27841 ggtgcagcgg aatttttgca cgggattatg gataaaggcc tgccgctggt gttgctgacc
    27901 aactatcctt cgcagaccgg gcaagatctg gcgaaccgct ttgccactgc cggtgtcgat
    27961 gtgccggata gcgtgtttta tacctctgcg atggcgaccg ccgatttcct gcgtcgccag
    28021 gaaggcaaga aagcatatgt ggtgggcgaa ggcgcactga ttcatgaact gtacaaagcc
    28081 ggtttcacta ttaccgatgt gaaccctgat ttcgtgattg ttggcgaaac gcgttcctac
    28141 aactgggaca tgatgcataa agcggcctat ttcgtcgcta acggtgcacg ctttatcgcc
    28201 accaacccgg acacccacgg gcgcggtttt tatcccgctt gtggcgcgtt gtgtgcaggg
    28261 attgagaaaa tctccgggcg caaaccgttc tatgttggta agcccagccc gtggatcatc
    28321 cgcgcagcat taaacaaaat gcaggcgcat tcggaagaaa cggtgattgt cggcgataac
    28381 ttgcgcaccg acattctggc gggcttccag gcaggtctgg aaacgattct ggtgctttct
    28441 ggtgtttcat cgctcgacga tatcgacagt atgccgttcc gcccaagctg gatttacccg
    28501 tcagtcgctg aaatcgacgt tatctgaata ccacccacgt ctgcggacgt ggttggctct
    28561 gatgacaaac gccaaactgc ctgatgcgct acgcttatca ggcctacgca gctcctgcaa
    28621 tatattgaat tttcatgctt ttgtaggccg gataaggcgt tcacgccgca tccggcatga
    28681 acaaatcgca ctttatcagc aatttggccc
//
LOCUS       DAJTVK010000044        28679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000044 DAJTVK010000000
VERSION     DAJTVK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.044"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1046)
                     /locus_tag="OO372_001955"
     CDS             complement(<1..1046)
                     /locus_tag="OO372_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6341458.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="OO372_001956"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="OO372_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="OO372_001957"
     CDS             complement(2433..3281)
                     /locus_tag="OO372_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6341459.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="OO372_001958"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="OO372_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6341460.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="OO372_001959"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="OO372_001959"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6341461.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO372_001960"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="OO372_001960"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6341462.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="OO372_001961"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="OO372_001961"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6341463.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="OO372_001962"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="OO372_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6341464.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="OO372_001963"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="OO372_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6341465.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="OO372_001964"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="OO372_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6341466.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="OO372_001965"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="OO372_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6341467.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO372_001966"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="OO372_001966"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6341468.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="OO372_001967"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="OO372_001967"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6341469.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="OO372_001968"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="OO372_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="OO372_001969"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="OO372_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="OO372_001970"
     CDS             22669..23652
                     /locus_tag="OO372_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6341470.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="OO372_001971"
     CDS             23675..25186
                     /locus_tag="OO372_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6341471.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="OO372_001972"
     CDS             25211..26209
                     /locus_tag="OO372_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6341472.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="OO372_001973"
     CDS             26242..27336
                     /locus_tag="OO372_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6341473.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="OO372_001974"
     CDS             27348..28220
                     /locus_tag="OO372_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6341474.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28679)
                     /locus_tag="OO372_001975"
     CDS             complement(28268..>28679)
                     /locus_tag="OO372_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6341475.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6819 a         7092 c         7789 g         6979 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagaca ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagagacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatat ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacgt
//
LOCUS       DAJTVK010000045        27614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000045 DAJTVK010000000
VERSION     DAJTVK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.045"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..145
                     /locus_tag="OO372_001976"
     CDS             <1..145
                     /locus_tag="OO372_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6341476.1"
                     /translation="LRRDRPDIAVRYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQ
                     TPG"
     gene            complement(276..1599)
                     /locus_tag="OO372_001977"
                     /pseudo
     CDS             complement(276..1599)
                     /locus_tag="OO372_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1599..3215)
                     /locus_tag="OO372_001978"
     CDS             complement(1599..3215)
                     /locus_tag="OO372_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6341477.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3511..4227
                     /locus_tag="OO372_001979"
     CDS             3511..4227
                     /locus_tag="OO372_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6341478.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4224..5885)
                     /locus_tag="OO372_001980"
     CDS             complement(4224..5885)
                     /locus_tag="OO372_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6341479.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6081..6509)
                     /gene="ibpB"
                     /locus_tag="OO372_001981"
     CDS             complement(6081..6509)
                     /gene="ibpB"
                     /locus_tag="OO372_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6341480.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6621..7034)
                     /gene="ibpA"
                     /locus_tag="OO372_001982"
     CDS             complement(6621..7034)
                     /gene="ibpA"
                     /locus_tag="OO372_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6341481.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7028..7100)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7340..7672
                     /locus_tag="OO372_001983"
     CDS             7340..7672
                     /locus_tag="OO372_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6341482.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7674..8888)
                     /locus_tag="OO372_001984"
     CDS             complement(7674..8888)
                     /locus_tag="OO372_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6341483.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            8989..10053
                     /gene="cbrA"
                     /locus_tag="OO372_001985"
     CDS             8989..10053
                     /gene="cbrA"
                     /locus_tag="OO372_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6341484.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10050..11387)
                     /locus_tag="OO372_001986"
     CDS             complement(10050..11387)
                     /locus_tag="OO372_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6341485.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11462..12610)
                     /gene="dgoD"
                     /locus_tag="OO372_001987"
                     /pseudo
     CDS             complement(11462..12610)
                     /gene="dgoD"
                     /locus_tag="OO372_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12607..13224)
                     /gene="dgoA"
                     /locus_tag="OO372_001988"
     CDS             complement(12607..13224)
                     /gene="dgoA"
                     /locus_tag="OO372_001988"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6341486.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13208..14086)
                     /gene="dgoK"
                     /locus_tag="OO372_001989"
     CDS             complement(13208..14086)
                     /gene="dgoK"
                     /locus_tag="OO372_001989"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6341487.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14083..14772)
                     /gene="dgoR"
                     /locus_tag="OO372_001990"
     CDS             complement(14083..14772)
                     /gene="dgoR"
                     /locus_tag="OO372_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6341488.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            14741..15373
                     /locus_tag="OO372_001991"
                     /pseudo
     CDS             14741..15373
                     /locus_tag="OO372_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15419..16231)
                     /gene="yidA"
                     /locus_tag="OO372_001992"
     CDS             complement(15419..16231)
                     /gene="yidA"
                     /locus_tag="OO372_001992"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6341489.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16346..16744)
                     /locus_tag="OO372_001993"
     CDS             complement(16346..16744)
                     /locus_tag="OO372_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6341490.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(16984..19398)
                     /gene="gyrB"
                     /locus_tag="OO372_001994"
     CDS             complement(16984..19398)
                     /gene="gyrB"
                     /locus_tag="OO372_001994"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6341491.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19427..20500)
                     /gene="recF"
                     /locus_tag="OO372_001995"
     CDS             complement(19427..20500)
                     /gene="recF"
                     /locus_tag="OO372_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6341492.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20500..21600)
                     /gene="dnaN"
                     /locus_tag="OO372_001996"
     CDS             complement(20500..21600)
                     /gene="dnaN"
                     /locus_tag="OO372_001996"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6341493.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(21605..23008)
                     /gene="dnaA"
                     /locus_tag="OO372_001997"
     CDS             complement(21605..23008)
                     /gene="dnaA"
                     /locus_tag="OO372_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6341494.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23305..23385
                     /locus_tag="OO372_001998"
     CDS             23305..23385
                     /locus_tag="OO372_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341495.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            23615..23755
                     /gene="rpmH"
                     /locus_tag="OO372_001999"
     CDS             23615..23755
                     /gene="rpmH"
                     /locus_tag="OO372_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6341496.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            23772..24131
                     /gene="rnpA"
                     /locus_tag="OO372_002000"
     CDS             23772..24131
                     /gene="rnpA"
                     /locus_tag="OO372_002000"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6341497.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24095..24352
                     /gene="yidD"
                     /locus_tag="OO372_002001"
     CDS             24095..24352
                     /gene="yidD"
                     /locus_tag="OO372_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6341498.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24355..26001
                     /gene="yidC"
                     /locus_tag="OO372_002002"
     CDS             24355..26001
                     /gene="yidC"
                     /locus_tag="OO372_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6341499.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26107..27471
                     /gene="mnmE"
                     /locus_tag="OO372_002003"
     CDS             26107..27471
                     /gene="mnmE"
                     /locus_tag="OO372_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6341500.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6634 a         7484 c         7053 g         6443 t
ORIGIN      
        1 actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag
       61 tcctggctat tttgccaata gctgcggagg tgcgccaata tccgtcatcc gccaatacat
      121 tgaacaacag caaacacctg gttaggtgga aaaccgcgcc ttatatcccc gccctgaagg
      181 acggggtttt acggcgcacc ggataaattt ggttatgcgc cgtcagccgt gctaaagaat
      241 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
      301 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
      361 ttcgataacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
      421 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
      481 ccgaccacca gtggttctgg tccgttgtgc ccgaccagac acgggatctc gaccatcgct
      541 tcatcatcaa agttatggat tgccccgttg ttaggcacaa tcagcaacat ccgctcctga
      601 gtgttgaagg caattgccgc cgccagatcg acgatgtatg acgcatgttc gtcaatctcc
      661 agcttggccg ccgctgaatt tcctgccgct gtaatggcgc ggcaagcatc gaaaacctgt
      721 ttctcgcgat gttccattca cctcattcgc gcgggtatgt tcagggttgg aatgctgaac
      781 aacataatcc gggaagagat aatatttcag ataggtgttc ggcaatgtat ccgggtccag
      841 tgcctggaca tcccgcgctt ttgcataggt gtcgttccag ctcgcttcag tatgtgtatc
      901 ttgctgcggt ggaacataac cgtgcttcga gacatattgc cgcagctggg gcatcaggtc
      961 gttgccctgc aaatcttcaa ttgatgtcca ccagccaaag tggttcaggc cgtagtagcg
     1021 cacgcgcatc tgtttgcgat cttgcagccc aacaatttgc gccatccggc tttcaatacc
     1081 gattggcatg tcacagatgt tgaggatttt cgcattcggg cgcagacgac gcgtggcttc
     1141 tgcgacaatg gctgccgggt tggagtagtt gagcatccag gcatttggtg aatatttttc
     1201 catataatcc accagttcca ggacgccgcc aatggaacgc atgccgtacg ctattccgcc
     1261 gggtccgcaa gtttcctgac caacaacacc gtggcgcagt gggatttttt catccagttc
     1321 gcgcatcggg tacttgccga cgcggatgtg cgccatgaca aaatcaacgt cgctgaacgc
     1381 cacttcagga tcggtggtgt aactaaacgc aatgtccggc gctttctctt taaggatgac
     1441 tttacaggct tcggcaatca cttcctgccg cgcgccatcg ttatcataaa atttcagtgc
     1501 acgaagcggg aaacgctcct gattcgccag gagcatcaac acgatgcctg gcgtaaaggt
     1561 gcttccaccg cctgcgacaa ccactgagaa tttggtcatt atactgcctc cgtaatggca
     1621 acattttctg ctgattgatg agaattaatt aagctatcga gctgttcacg cagctgggat
     1681 acatgcagac caatgatcac ctgaatggca tcgccactac ggaagacgcc gtgcgctccc
     1741 agctttttaa agacttcgtc atccagcgtt tgtgacatgt catgcagtgc aatgcgtaga
     1801 cgcgtcgcac aattgttaat gctggagata ttgccgaccc cgcccagggc ttgcaggata
     1861 ccggcagcct gatccagctc tttttttggc tctgccgcgg tggtttggcc tcgcgaggct
     1921 ttgtattcgg cttttgagta gagtttcact tccgcatctt cacgtcccgg cgtgcacatg
     1981 ttgaactgca gaatcagggt gcggaaaacc acgaagtaca gcagggtaaa gcacaaccca
     2041 atggcgattt gggtcagcat catatccgcg tggttgctga acatcgggat ccagttttgc
     2101 ggtaaaacct ggtcaatcag acctccgccc atgttgccca ccacaccaaa gagatacatc
     2161 acggtcgaca ttgaggccgc aagcacagcg tgtaccgcaa acagcaacgg tgaaatgaac
     2221 aggaaggtaa attccagcgg ttcggtaatt cccaccagca tggcggttaa ggttgcggga
     2281 atcagcaagc ccgccacttt tacccgattt tccggtgcgg cagtgaagta catcgctaaa
     2341 gaaatgccca cggcaccaaa gatttttgag ttaccgtgca gggcaaaacc gccttccggg
     2401 aacaacgatt tcagcggctc ggcgctcaaa ctgaactctt gcagatgctg cgcccagtac
     2461 atctgaatgc cgccttcaac agctgccgga cccaagataa acggtccgta gatgaagtgg
     2521 tgtaaaccgg ttgggatcag aatacgttcg aggaaggtgt aaacccagac cccaagcgca
     2581 cccgccgaac gcaggaacgc ttgcagagat tcaatccccg tttgtacttt tggccagccg
     2641 agcaatgtca gccaggcaca ggggatcatc accaggaagg cgataatcac cacataagac
     2701 gtcccctgga aaatgccgag aaaaaccggc agttttttat cgaacagacg gttatgcagc
     2761 gccgtcacaa tgccggaaat gataattgcg ccgataatgc tggtatcgag ggttttaatc
     2821 ccggccatca ttgtcagacc gctacctgcc accgcgtcct gagtgaaatc gacgccgaag
     2881 tagcttcccc aggtcattcc catcgcgttg atgaaatagt tccaggtcag gaaactcacc
     2941 atcaccgcca gacaagcacg tccctgcgct tgcttagcaa ggccaatggg taaaccgaca
     3001 gcaaaaatca gcggcatatt acggaatacc gtccaaccgc cctcttcaat aatgtgtacg
     3061 atttgcgcga ataaactgtt cggatcggtc agtgattccc cgacaaacat cgggttttgc
     3121 agcaagatgg caagacccac cacaatcccg gcgaagggaa acagcagcac tggcgtaaac
     3181 atcgcgccgc caaagcgttg aatttgactg agcatttcca catcctcttt tctcaattct
     3241 gtaagtagga agcctgattg gttatgcggc aggcctggag caaggatagg aagatgcgat
     3301 cggcagaaca tgacgaccca atgggattcg tgatcgcttt catgcttttc gttgggcggt
     3361 agagatctac gttttcgcga aaaagtagac atctgtgagc acaggtacac agcatcgaac
     3421 acatctttaa aaaaaagatg ttttttcaat cgattaagca gaacttgtgt gcgcattacc
     3481 cgggcttgca ggcaaaaaag agatctagag atgatctaca aaagcattgc ggagcggtta
     3541 agaattcgac ttaactccgc agatttcacg ctaaacagcc ttcttccggg tgaaaaaaag
     3601 ctggcggaag agtttgcggt atcgcggatg accatccgta aagccattga cctgctggta
     3661 gcgtgggggc tggtggtccg ccgccacggt agtggcactt acctggtgcg caaagatgta
     3721 ctgcatcaaa ccgccagtct gaccgggctg gtggaggtgt taaaacggca gggaaaaact
     3781 gtcaccagcc aggtgctgat ttttgaaatc atgcctgcgc ctccggccat tgccagccag
     3841 ttacggattc aaatcaacga gcagatctac ttctcccgtc gcgttcgttt tgtggaaggg
     3901 aaaccgctga tgctggaaga cagctatatg ccggtaaaac tgttccgtaa tctttcgctg
     3961 caacatctgg aagggtcgaa gtttgaatat attgaacaag agtgcgggat tttgattggc
     4021 ggtaattatg aaagcctgac gccggtgctc gccgatagac tgctggcgcg gcaaatgaag
     4081 gtagcggaac acacgccact gctgcggatc acctcgttgt catatagcga gagcggggag
     4141 tttttgaatt attcagtgat gttcagaaat gccagcgaat accaggtgga gtaccattta
     4201 cggcgactcc acccggaaaa gagttaaccg atactccaga agagcaccgc cagtaattgg
     4261 ggggtgataa tgcgcaggaa catcactaac ggatagacag tggcgtaaga gagcgccgcc
     4321 gcaccgctag ttggatgaag attattagca aacgccagcg ccggaggatc ggtcatggaa
     4381 cctgccagca tcccgcacat ggtcaggtaa ttcattttgg ctaacatccg cgccagaatg
     4441 ccaacagtaa tcagcggaac ggcggtgatc agggcaccat aaccaatcca gcttagccct
     4501 tcgccattga ccagggtatt cacaaaatcc ccaccagatt tcagaccaac gaccgagagg
     4561 aacagcacga tccccagctc ccgcagcgcg aggttggcac ttggcggcat aaaccagtac
     4621 agcttgccga tactgccgat acgcccgagg atcaacgcca taatcagcag accgcccgcc
     4681 agccccagtt tcaacgcggc cgggaatccc ggcacaaaga cgggaataga acctaacaat
     4741 acgccaagcc cgatgccaat aaacactggc agcatctgaa cctgttgcag tttttgctgc
     4801 gcattcccca gcacattggc aacggcatca attgcggacg gacgccccac caggttgagg
     4861 atatcgccga actgcaggct gatatcgcca ctggcgacca gttcgacccc ggcacggttc
     4921 aggcgcgaga taacaacgtc atagcgttct ttaaagtgca ggtcgcgaat acgttttccg
     4981 agcacgtttt cattggtgac caccacacgc tcgacgcgca aatcagtgtc tttcgtggac
     5041 agcgaagtat cgacctcctg accaatcacc agttgcgcat tatgtaaatc cgctggctga
     5101 cccaccagat gcagcaaatc gcccagttgg ataatggtat ctggcgaagg aacttttagg
     5161 gtttcttcgc gtttcagacg cgagcagata attttgtcgc cgttgagaat cggtacatct
     5221 ttaatggcta aatcatgcag gttagggttc tcaacgcgaa tattgatagt cttgatcagc
     5281 gcgccgccat tggtgcgtga agactcgtgc tgctgagctt ctgtctcgac attgacgcgg
     5341 aaaataaccc gcaacatcca catggtgaac aaaatcccgc aaatgccgaa tggatacgcc
     5401 atcgcgtaac tcatccccat ctgatcgacc atttccattg gtgtacccag gtcgcgcaaa
     5461 atctgctgcc ctgcccccag cgctggcgta ttggtaaccg caccggagaa aatccccagc
     5521 actaccggca gtggaatatc aaacagttta tgcaggatgg cggtaaccag accaccgatg
     5581 atgacgatca gaacagcaaa caggttgagg cgtaatccgg agacgcgcaa tgaggcaaag
     5641 aagcccggcc ctacctgaat cccgatggta taaacgaaca ggatcaggcc aaattcctga
     5701 ataacatgca gcatatcgct acttaatgtc atccccgcct gagaaacaaa atggccgacg
     5761 atgatcccac caaacagcac gccgccaata cctaatccta tgccgcgaaa tttgacgttg
     5821 ccgataaaca aaccgacgac tgccaccaaa gccagaatac tgaccgttaa tgctatatca
     5881 ctcatcgtcc atttctcttg agataacatt ttaattgcta cggattatgg ctgaatcggt
     5941 gaagcagatt tgccttatag cgcacattat tacgggaaag aacgtgccga aatatcttaa
     6001 acagtcgaaa tttaacgcat tgttgaagaa gaaagacaaa aaaagcccca ccagaatggc
     6061 ggggcaaaga gaatagctag ttagctattt aacgcgggac gttcgctgat agcgatacgc
     6121 tgcgctgcga tgggttcagg ctcattacga attaaatcaa tatgcagtaa accgtttacg
     6181 aaagttgcgc cagagacttc catattttca gccagcgtaa agctcaggct aaatggctga
     6241 ttcataagcc cttgatgcag ccattttttc tcttcttttg gctgctccgg cgtgcctttt
     6301 acgctcaggc gcgtaccttc cagttgaatc tctaaatctt cctgacggaa acctgccagc
     6361 gcaagggtaa tgcggtagtg gttatcgtcg cttttctcaa tgttgtacgg cgggaagctc
     6421 tgactttcac cggcgttttg cagtgcgttg gccagtttgt caaaaccgat ccattgacgc
     6481 atcagtgggg ataaatcgaa gttacgcata gtcatttctc cttctaagaa gcgagtaagt
     6541 acctgcaaat ccgaagattc gcatatgctc cctgacggcg agcatggaga tgtcaggccg
     6601 cgccaggcgg ccttaaggaa ttagttgatt tcgatacggc gcggtttttt cgcttccgga
     6661 atcacgcgtt cgagatcgat atacagcaaa ccatttacca ggttagcgcc acgaacatga
     6721 atgttctcag ctaactggaa tttgcgttca aagttgcgtt cagcgatgcc ctggtacaga
     6781 taggtgcgct ctttttgttc gtcggcgtga gcacctttca ccaccagcag attatcctgg
     6841 gcggtaattt ccagttcgct ctcagcaaaa ccagccacag cgatagcaat gcggtaatgg
     6901 ttttcgtcta ccagttcaac gttatacgga gggtagccgc cattactctg gctctggttg
     6961 ttttctaagt ggttaaacaa acggtcaaat ccaatagcag aacggtaaag cggggataaa
     7021 tcaaagttac gcataatcaa tagctcctga aatcagcgag aatgtaagac cttccacaat
     7081 ggacaggtca ggtagccaga acacccatca ggcgcgtcct catcgactac gatgtaaaaa
     7141 tgggtctgga aatgaacttt tcaagcctta tcagcggact tttttttgca gtttatggtc
     7201 tattgcatag actgaggggg cagcacgaaa cgttaaagtg tgatagccgc cacagagcga
     7261 cgtaatgatg atgttatttt cagcaaggga tcgctataac tcatcaggcc aacacccacg
     7321 atcaacagat gaatttatta tgataagaaa tgtgttgtta gcgttcatga tatgcagcgg
     7381 aatggcatta ctcggaggat gctccagcgt gatgtcccat accggaggga aagaaggaac
     7441 gtatcccggc acccgcgcca gcgccaccat gattggcgat gatgaaacca actggggcac
     7501 taagtcgctg gcgattctcg atatgccgtt taccgctgtg atggacacgc ttttattacc
     7561 gtgggatgtg ttccgcaaag acagctcggt gcgctcacgc gttgaaaaaa gcgaagccaa
     7621 cgcgcaagcc accaatgcgg ttattccacc ggccagaatg cctgataact gatttagcga
     7681 tcagtttcgg tgatccacag ctggccacct tccatccacg ccagccattg accatccggc
     7741 gagaagacca cggcgtccgc agaaggtgga ttggcgtggt tttcggttaa atactcaacc
     7801 tcgccgcttt gcgcatgggc acaagcaatt cgattatcca gcacaaagcc caaccattct
     7861 cctgacggat gccagttaaa tgcagactga atatccgttt tgttatgggt taactggcgc
     7921 ggctcgccgc cctgtggcga gataagccac agttgcacaa tgccgttatc atcacgcatt
     7981 aaaaacgcga tttgcgtacc ctgcggatta cagcgcaccc agtggcgcgg gacgttgact
     8041 aaccccggat aagcccgatg gtgggtaaag gttaaacgtc gctgcacgac gccacgcggt
     8101 ggcgcgggca gggtggtttc cgttccactt aacggcgcat cacctgccgc tttccagcca
     8161 gcttcatctt gcggcaactc aacgataaac agctccggca ctttctcacc ctttggcgaa
     8221 agtgtgtcgc caataaacgc cagcgcgtga tttcctaccc atccttcttc ataagcacga
     8281 ttgatttcat cactgccagg ctgcggcgtg ggcgtggttt tactcaccag cacacaccag
     8341 tggctgccgc tgtattcacg cggatgctgc ttttgtacgt tgaccggacc aaacggcgca
     8401 gcaacaccga cgtttcgcaa atccagcgcc ggatcgagtt catgcattac atggtcgtta
     8461 taggtaaagc tcaccctttc accgttcggg ctaaagacat gcacatggct gccgccgcgc
     8521 agcgctcctg gcgtatacgg cgcggtaata tccattgcat cgagattgct caccttgccg
     8581 ccttcagcaa tgactccgcg ccgatgatgg aagtcgtaat gccatgtttc atcaggattt
     8641 tccgggccgt gaataaagac atatttctct gacttcggat gaacggtcac cacgccgacg
     8701 tgtgcgccct gtgacgcaca gtagataacc tcgacctcac cggtatggat attcacacgc
     8761 tcaatggtct cgccggtaaa cgacgcccca gaaggacgca cgtcaaatac cagccactgg
     8821 ctgtcgggcg tccaggtatt ggtattggtg agcaggtgat tacggggagc aaaggttatc
     8881 tgtttcatgg cgcggtcctg atacaaaacg ggtccagcca tcatacttca caaggacttc
     8941 actttcagac aatagtcttt tgccatcgtg atgaatgtga gtgaaaagat ggaacatttc
     9001 gacgtggcga ttattggcct cggcccggca gggtcggcgt tggcacgaaa gttagccggc
     9061 aaaatgcagg tgatcgcgct ggataaaaag caccagcatg gtactgaagg tttcagcaaa
     9121 ccttgtggcg gtctgctggc accggacgcg cagcgatctt ttattcgcga tgggctgacg
     9181 cttcctgtcg atgtgatcgc taatccgcag attttcagcg tcaaaaccgt cgacgtcgcc
     9241 gcatcgctca cgcgtaacta ccagcgaagc tatatcaata ttaatcgcca tgctttcgac
     9301 ttgtggatga aatcgctgat ccccgccagc gttgaggttt atcacgacag cctgtgtcga
     9361 aaaatctggc gtgaggatga taaatggcat gtcatttttc gtgcagacgg ctgggagcag
     9421 catattaccg cccgctatct ggtcggtgcc gatggtgcca actcgatggt gcggcgacat
     9481 ctctacccgg atcatcaaat ccgtaaatat gtcgcaatcc agcagtggtt cgcagagaaa
     9541 catccggtgc cgttctactc ctgcatcttt gataatgcga taactgactg ttattcatgg
     9601 agtatcagca aagacggtta ttttatcttt ggcggtgcct atccaatgaa agacggtcag
     9661 acgcgtttca cgactctgaa agagaaaatg agcgcctttc agttccagtt tggcaaagcg
     9721 gtgaaaagcg aaaaatgcac ggtgctgttt ccctcacgct ggcaggattt tgtctgcggt
     9781 aaggacaacg cctttctgat tggtgaagcg gcgggattta tcagcgccag ctcgctggag
     9841 gggattagct atgcgctgga tagcgcagac attctgcgtt cggtgttact gaagcagcca
     9901 gagaagctca ataccgctta ctggcgcgcc acccgcaaac tgcgtttaaa actcttcggc
     9961 aagatagtaa aaagccgatg cctgaccgca ccggctttaa gaaagtggat tatgcgcagt
    10021 ggtgtggcac atattccaca gttgaaggat tagccaacgc gcttcacatc gcccaccagc
    10081 aggatataag agagcgcgcc aatcaacgcg acggcggaga tataaaccag tgcaggtgcg
    10141 aaaccgtaac cctgcgccag gtaccccacc accagcggaa cggtgatgcc gcccagacca
    10201 ccggcgaagt taaacacgcc gccggttaaa ccaatcaggc gcatcggtgc cagagaagaa
    10261 accagcgacc aggtaattga agcaaaaccg ttaccgaaga atgccagcgc catcaggcac
    10321 ataatcatca tcggatcgtt agtgtagtta gcgcccataa tgcaggtgga gatcagcaag
    10381 ccgcagataa tcggcgtttt acgcgcaaag cccagtgaaa agcccttacg taccagcaga
    10441 tccgctaccc agccagagag caggacgccg acaaacgccg cgaggaatgg caccgtggtc
    10501 ataaagcctg ctttcagcgc cgtgattcct ttttcctggg ttaaatagtt cgggaaccag
    10561 gttaagaaaa accacagtgt agaagccacc gcaaattgcc caagatagac gccgatcagt
    10621 ttacgatgga acaccagttt ccagtctttg gctgttaacg gctgacgtgc ctctttcttc
    10681 actggcgcat caccatccac cagaccgccg ccatcacgaa tgtaatccag ttcagctttg
    10741 ctgatgcctt tggtcaggcg cggcggctga taaaccttaa accaaatcag cgaccagata
    10801 atgccgatac caccagtcac aatgaacacc cagtgccagc tcaacatctc ctgaatccag
    10861 atcagcagcg gagtcaaaaa cgccagaccg acaaactgac cagacgtata aaaaccaacg
    10921 gcggaggcgc gttcatgttc cgggaaccag ctggtcacca tccggttatt ggtcgggaaa
    10981 gcgggcgctt cgaaaatacc ggttatcgca cgcagaccaa ttaatgacat taatcccgtg
    11041 gcaaagccct ggaataaagt cgccactgac cagccaaata tcgcaataaa ataagtcacg
    11101 cgagaaccta cgcgatctaa aaaccaaccg ccggggatct ggcatagcgt ataaagccag
    11161 gcgaaggccg aaaatacata gcccatttcc gctttcgtaa tgccgaactc ttcctgaata
    11221 tgggcggaag ccacggccag gttagcgcgg tcaacataac aaatgaccac cgtaataaag
    11281 atcatcacca gcgtcagata acgccgacgc cccggctttg ctgcattaac gggaatatcc
    11341 atagcgagct ttctccagat tttgggcata gcgaagccgc tcaccatgcc ctgtaattta
    11401 cagagggtta tatttttgta ttgctgtttt agtgcccgat gaggggctta cgtggcagga
    11461 attaccactc tgctacgctg ttatcttcat gacgccagag tggattacgc cagtccgggg
    11521 catttttact gaactcaata actttagctt cgtcgatttc cacgcctaag cccggtttcg
    11581 ttagcggttt aaagaagccg ccaaccatgc tgaagtcttc tttgtttttc acaaagtcga
    11641 gtaactccgc gcctttgttg taatgaattc ccatactttg ttcctgaagt accgcgttat
    11701 aggaaacaaa gtcgatatgc agacaagccg ccagtgcaat cggtccgagc ggacagtgcg
    11761 gcgcaagggt cacgtcatag gcttctgcca ttccggcaat tttgtagcat tcggtaatac
    11821 cgcctgcatg ggagagatcc ggttgtagaa tcgaaatacc gcctgcctcc agcacgcgtt
    11881 taaaatcgaa acgtgagaac atgcgctcac ccgccgccag tggaatatgc gtttgtgccg
    11941 ccaatttcgg gtagtattcg gcttgttccg ccagcaccgg ctcttcaata aacagcgggc
    12001 gatacggctc cagttcttta atcagcactt tcgccattgg cgcgctgacg cgaccgtgga
    12061 aatcaagacc aaactcaatc tgattgccaa aagcttcacg aatttgtgcc acggtgttga
    12121 ctgccgcatc taccgcgcgg gagttatcaa ttagccccag ttcttcacaa ccgttcagtt
    12181 tgaaggtatc gaagccgatt tcgcgcaggg ttttaatacc gtcgatcaca tccgccggac
    12241 gatcgccgcc gacccaactg taggctttaa ttttgtcgcg aaccaggccg cccatcagtt
    12301 gccagaccga cgcattcagc actttacctt tgatatccca taacgcctgg tcgatcccgg
    12361 caatggcgct catcaggatt ggaccgccac gataaaatcc ggcgcgatac atcacttgcc
    12421 ataagtcatt gatgcgctat ggatcctgac caatcaaata gtcacccagc tcgtgaactg
    12481 ccgcttccac cgtgcgggcg cggccttcaa tcactggctc gccccagccg acgacgcctt
    12541 catcggtttc aattttcagg aacatccagc ggggaggtaa acgatacgtg gtaattttgg
    12601 tgattttcat tgcactgcct ctcgatacgc cttaacaaat gctgctgcct gctgcgcggt
    12661 gcgttctacg gactgcccgg cgcgatagag atcgctgcct aagcccgccc cagcacaacc
    12721 tgcgtctatc cactgcgcca ggttttctgg cgtcacgccg ccaacggcaa agactgcgat
    12781 gtccgatggc aataccgctt ttaacgcttt gatgtattgc ggaccaaaag ccgatgacgg
    12841 aaatattttc agcgcctgcg cgcccgcttc gagcgcggta aaggcttcgg tcgccgtcgc
    12901 gcagccgggg cagacggtca tgccgtagcc caccgcacgg cggatcactt cactatggat
    12961 attgggcgta acgatgagct gacagcccat cctggcgagc gcatcgacct gttcaggttt
    13021 cagtaccgta cctgcgccaa tcaacgcctt gtcgccatac gcatcaacga tggcgggaat
    13081 gctttgctcc cattgtgggg aattcagcgg gatttcaacc gcgtcgaacc cggcgtcaat
    13141 caccgcgcca acatgcgcca gcgcctcgtc gggcttaatg ccgcgcaaaa tggcgatcag
    13201 cggaagttta gtttgccact gcatgagcga tgctccttat accagcctga aatgccgtgt
    13261 cgcccgccac cgccgtcacg tcgcaaccca tcgcctgaaa ggcttgctgg tagcgcgcgg
    13321 tcaacgatgt tccggcgaca agggtgatgg cgtgttgatg ggccacatag tcgcgcatac
    13381 tggcgacctc tgcgccaatc aacaaaccag agagaaattc actgacctgt tcacggggaa
    13441 gtgttcccag cacatgcgag gcacgaactt caaaaagctg cggcaatatg gcgggcgtat
    13501 taagaccacg ctcaaggcca gccgtgaagg catcggcaga gttttcctgc ggcggcaaac
    13561 ctgcaccaat caatgagtga tttaacagta aatgatgtaa ttcaccggtc atcacggtgc
    13621 gaaaatcgtt gatttgctgg ctatcggcct gcacccattt gcaatgcgta ccgggcatga
    13681 cataaagaga ggaaggagcc agagctcgcg cgccgatcaa ttgagtttct tcgccgcgca
    13741 tcacattgtg gttatcatca tgagagacgc ataatccggg aataatccag atattgtcgc
    13801 caacagacgt taattgttcg ccaatagacg aaaaatgggc aggaacagat aaatacggag
    13861 caactttcca gccgacattg ctgccgacca ttcctgccat taccactggc gtattctctt
    13921 cacgccagtc ggtcgtgact tctgctaaca ccgcagccgg agattttccg ttcaggcgcg
    13981 tgacgcctgc ttctgattgc ctgctctcca ggcagtggtc gccctgataa agccaggcgc
    14041 gcagattggt cgatccccag tcaattgcga tgtagcgagc tgtcatgtga tttcctttaa
    14101 ccttcgtgtc gagctggcga tcatggtaag cgccgcctgc tctgccgcat cgccgtcctg
    14161 atgccgtatc gcatcgaaca gcgccttatg ttcctggagc gtttgcggca tgttggcctc
    14221 atcgcccatc caggttcgtt caaaaaccgc ccgctgcagc gaactgatcg caatgctaag
    14281 ttgctgtaac accgggttat gcaccgactg cagcaccgcc tcgtggtagc gaatatccgc
    14341 ttcgttaaac gcttcgcggt cctgattgtt ggcaatcatc tcgttcagcg ccgattcaat
    14401 ctgcgccaga tcgctggagg tcgcgcgctc tgctgcccaa cgggcaatcg ccggttccac
    14461 cagatttcgc acttcgctca tggcactgat aagccgtggg tcgtagtcgt tttccagcac
    14521 ccattgcagt acgtcagtgt cgaggtaatt ccactggtta cgcggtgcca caaacgcgcc
    14581 gcgataacgt ttcatttcaa tcagccgttt cgccatcaac gaacggaaca cctcacggat
    14641 gatgttgcgc gaggttgcaa actcctcaca gagttccgcc tcagccggaa gcggcgagcc
    14701 tggtacgtat ttgccgtgaa cgatctgttt acccagcgta atgacgatgc gatcggtttt
    14761 attgagagtc atggagagtc cttgtgctct gtatgttctt ctctacttta ccccgatcga
    14821 tgcataatca ggaaagtatc aatcgcagtc agacaccatc acggggctat cgcaggcaaa
    14881 ggatagtaat ggaacaaaag gctatgtttt tgtaggggaa tcgctggatt accttatcac
    14941 agatggtgcc gatgacatcg ttaagatgct caaggatcca gctcttaacc ggcacaatat
    15001 tcaggttgct gatgacgcaa gatttgtttt aaatgcgggg aaaaagaaat ttaccggcac
    15061 aatatcactc tattacaact ggagcacaga agaagaaaaa gcacgagtga cacaatatcg
    15121 ttttgcttgt ggtgttcagc actgtacccg gacagtagaa aacctggaag gtactatcca
    15181 tgagaaaaat aaaaacatgg attactcaaa cgtaatggct ttctaccatc catttaaagt
    15241 gcgattttat gaataccatt cacccagagg cattccggat ggtgtttccg cagcattact
    15301 gccagtgact gttacgctgg acatcattac tgcaccgctg caatttctgg ttgtatatgc
    15361 agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc tgtttgcccg cccacagatt
    15421 aattcagcac atacttctca atagcaaacg ccacgccatc ttcaaggtta gatttggtga
    15481 caaagttcgc cacttctttc accgaaggaa tagcgttatc catcgccaca ccgacgcctg
    15541 catattcaat cattgcgatg tcgttttcct gatcgccaat cgccatgatt tcttccggtt
    15601 taatacctaa cacgtcggcc agtgatttca cacccgtacc tttgttaacg cgtttatcga
    15661 ggatttcgag gaagtacggc gcacttttca gcacggtata tttctctttc acttcctgcg
    15721 gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc aatcatcatc actttcagga
    15781 actgggtatt ggggtccatt ttctccgctt cgcagaacac cagcggaata gtggcaacga
    15841 aagattcatg cactgtgtag tagctgatat cgcggttggc ggtgtacagc gtggtgcggt
    15901 ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt ttccaggaaa cgatagtcgt
    15961 catagctgag agcagtttgc gccacggtgc taccatcagc tgccttctgt actagcgcgc
    16021 cgttataagt aatgcagtag tcgcccggct gttccatatg cagctctttc aggtagttgt
    16081 gcacacctgc atacgggcga cccgtcgtta gcacgacatt cacgccacgg gcgcgagctg
    16141 cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc gggcagcaga agggtgccat
    16201 ccatatcgat agcaatgagt ttgatagcca tgagttcccc aggtagattg gttcctgacc
    16261 catgctaacg cgattccgct caaaaatcag tacaacaccc gagggaaaag ggggatgcaa
    16321 cgcgggttgc atgctcccca tttgcttagc ggaagagttt ccctttcagc agttccatac
    16381 ctgcggaaag cagatcgtta ttggcttgtg gtgacacttc accttgcggt gagagcgcat
    16441 caataatctt cggcaattgc tctgccagta aactggaagc tgtactggta tccacgccaa
    16501 gtttttgccc gagatcggac accgcatttg tgccgagcgc cgattccagt tgctcgccac
    16561 taaccgattg attgccctgt tgattactca gccaggttga gagaatggcc cctaagccgc
    16621 cactttgcag tttttccagc agcacctgaa tgccgccctg ctcctcaacc caacttaaaa
    16681 tagcctgata tttccccgca tcgcctttca gaaaggcacc gacaacttca tcaaaaagcc
    16741 ccatgataat cacctgtaaa gcgttacgtg ttgacccaaa aagtatagat ttgcgggatg
    16801 ataattgcgg attgcagaaa taaaaagggc ggagatgatc tccgcccttt tcttatagct
    16861 tcttgccgga tgcggcgtga acgccttatc cggcctacaa aatcatgaaa attcaataca
    16921 ttgcaagatt ttcgtaggcc tgataagcgt agcgcatcag gcacgctcgc atggttagcg
    16981 ccattaaata tcgatattcg ccgctttcag ggcgttctct tcaataaacg cacggcgcgg
    17041 ttcaacggcg tcgcccatca gcgtggtgaa caactggtcg gcagcaatcg catctttaac
    17101 ggtaacgcgc agcatacgac ggctttccgg gtccatggtg gtttcccaca gctgttccgg
    17161 gttcatctcg cccagacctt tataacgctg gatggagagg ccgcgacggg actctttcac
    17221 cagccagtcc agcgcctgct cgaagctggc taccggctga cgacgctcgc cacgttcgat
    17281 aaacgcatct tcttccagca agccacgcag tttctcaccc agcgtgcaga tacgacgata
    17341 ttcgccaccg gtgataaact cgtgatccag cggatagtca gtatccacac cgtgggtacg
    17401 cacgcgaaca atcggttcaa acaggttttg ttcggcattg gtgtggacat caaacttcca
    17461 ctggctgccg tgctgttctt tgtcgttcag ttcgctgacc agcgcgttca cccagcgggt
    17521 aacggtctgc tcatcagaga ggtcggcttc cgtcagcgtc ggctgataga taagctcttt
    17581 cagcattgct ttcggataac gacgctccat gcggttgatc attttctgcg tcgcgttgta
    17641 ctcagacacc aatttctcta acgcttcgcc agccaatgcc ggtgcgctgg cgttggtgtg
    17701 cagcgttgcg ccatccagcg cgatagagat ctggtactga tccatcgctt cgtcgtcttt
    17761 aatgtactgt tcttgcttgc ctttcttcac tttgtacagc ggcggctgag cgatgtagac
    17821 gtgaccgcgt tcaacgattt ccggcatctg acgatagaaa aaggtcaaca gcagcgtacg
    17881 aatgtgcgag ccgtcgacgt ccgcatcggt catgatgatg atgctgtgat aacgcagttt
    17941 gtccgggttg tactcgtcac gaccgatacc acagccaagc gcggtgataa gcgtcgccac
    18001 ttcctgagaa gagagcatct tatcgaagcg cgctttctca acgttgagga ttttaccctt
    18061 cagcggcaga atcgcctggt tcttgcggtt acgcccctgc ttcgcagagc cgcccgcgga
    18121 gtccccttcc accaggtaca gttcggaaag cgccggatcg cgttcctggc agtctgccag
    18181 tttgcccggc aggcccgcta agtcgagcgc acctttacgg cgggtcattt cacgcgcgcg
    18241 acgcgccgct tcacgggcac gggcagcatc gataattttg ccaaccacga ttttcgcgtc
    18301 ggttgggttt tccagcaggt attctgccag cagttcgttc atctgctgtt caaccgctga
    18361 tttcacctca gaagaaacca gtttgtcttt ggtctgggag gagaatttcg ggtccggcac
    18421 tttcacggaa acgaccgcaa tcaggccttc acgcgcatcg tcaccggtgg cgctaacttt
    18481 ggcttttttg ctgtagcctt ctttgtccat gtaggcgttc agggtacggg tcatcgccgc
    18541 acggaagcct gccaggtgag taccgccgtc acgctgcgga atgttgttgg taaagcagta
    18601 gatgttttcc tggaagccat cgttccactg caacgccact tcgacgccaa taccgtcttt
    18661 ttcagtggag aagtagaaga tattcgggtg gatcggcgtt ttgttcttgt tcagatattc
    18721 aacgaacgcc ttgatgccgc cttcatagtg gaagtggtct tctttgccgt cgcgcttgtc
    18781 gcgcagacga atggaaacgc cggagttgag gaacgacaac tcacgcagac gtttcgccag
    18841 aatttcatat tcgaactcgg tcacattggt gaaggtttcg aggctgggcc agaaacgcac
    18901 catggtgccg gttttttcag tctcgccggt aaccgccaac ggtgcctgcg gtacaccgtg
    18961 ttcgtagatc tgacggtgaa ttttaccctc gcgctggata accagctcca gtttttgcga
    19021 cagggcgttt actaccgaaa caccaacgcc gtgcagaccg ccggacactt tataggagtt
    19081 atcgtcaaat ttaccgcctg cgtgcagaac ggtcatgatc acttccgccg ccgatacgcc
    19141 ctcttccggg tgaataccgg tcggaatgcc gcgcccgtca tcctgtacag agacagagtt
    19201 atcggcgtga atggtgacga taatttcttt acagtgaccc gcgagcgctt cgtcgatagc
    19261 gttatctacc acctcgaata ccatgtggtg cagaccggtg ccgtcatccg tgtcgccgat
    19321 atacataccc gggcgcttac gcaccgcatc cagccctttc aggactttga tactggagga
    19381 gtcataagaa ttcgacatca acgtttctcg ctcatttata cttgggttaa tccgttattt
    19441 tacccttttc cacggtaaac atcttcgaat tttcgtccga catgtctata acgtgttcag
    19501 cactgatcgc gctgacaaag acctgtgatt gcgtcgcttt taagcggctg gcaagcagcc
    19561 cgcgacgctc atcatcaagc tcagaggcaa aatcatctat caggtagaga caccgccgcc
    19621 cgctttcacg ggtgaggaac tctccttgcg ccagacgtaa ggcgcacatc aacagcttaa
    19681 gctgcccacg cgataaggta tcttccaccg gcgcaccgtc ggcgcgaatg cgtaagtccg
    19741 ctttatgcgg gccatgcgcg gtgtaggtta gctggcgatc gcgttcaaaa ttacgttcca
    19801 gcacctcagc atattctgtc tctttctccc agccgcgctg gaaagagaaa gtcagagaaa
    19861 actcagggag aaattgctta caagtatcgg ccatatcagc cgcgataccg gcgctatact
    19921 ccgcgcgcca ggtgctgatt tgctccgcca gcgggatcag ctctttatcc cacgggcgta
    19981 gctgttcgta acgtgtcacc tggcgcagcg ccgcattgcg ctgcttgagc aatcgcttga
    20041 gattgctcca ggcggtgaaa aatccgggtt cgttgtgaaa gcatccccag tcgaggaatg
    20101 ctcttctgta tttggggccg ccgttgagta aagtaaaccc ttctggcgtt atcaactgca
    20161 ttggcatcag gtgcgccagt tccgcgacct tatgcccgtc tgtaccgtcg atgcggactt
    20221 tgctgtcgcc ctgtttgtct ttggttaagc caatcgctgt ctcgcgctct tcgccctgta
    20281 atcgcccgtg gagaacaaac gcctcctgct catggcgaat gacgcgacca atctgcaaac
    20341 tgcgaaacgc ccgaccatgg ccgagcgtat agatggcttc cagcacgctg gttttgccac
    20401 tgccgttggc acctaccaga aagttaaagc cgggagataa agcgagatcc gcggtttcaa
    20461 tgttgcggaa atcgcggatc aacaagcggg tgagggacat tacagtctca ttggcatgac
    20521 aacataggca gccgactgtg atgcggcatc ttcaatctgc acgctggaaa ccgaatcggt
    20581 cagcatcatg cggacgtttt cacatttcag cgcgttcaga acatccagca cgtagctgac
    20641 gttgaagccg atttccatct ccgcaccgct ataggtaacg tcgaggatct cttccgcttc
    20701 ttcctgttcc gggttgttgg cggtgatttt cagctggttt tcgctgacat aaagacgtac
    20761 gccgcggaat ttctcgttag agagaatcgc cgcgcgagca aacgcctgct tgagcagatc
    20821 gcagccagct tccagatgtt tgtccgggtt cttcggcaga acgcggcgat aatccgggaa
    20881 gcgaccatcc accagtttgg aggtgaagat aaagtcgcca acgtgggcgc gaatgttgtt
    20941 gctgccaatc tgtacgcgca gcggattgtc gccgccgtcg agcatacgca tcagttcaat
    21001 cacgccttta cgcggcacga tcaccgaatg gcttggcaaa gattgaccaa ttggcattga
    21061 acagactgcc agacggtggc cgtcggtcgc cacggtgcgc agttcttcgc cttcggtttc
    21121 aaacagcata ccgtttaagt aatagcgaac gtcctgatgc gccatagaaa actgggtcgc
    21181 ttcaatcaga cgcttcatcg tcgcctgcgg cagggtaaat tcgacttcgc tctgccagtc
    21241 atcgaggttc gggaaatccg ccgctggcag ggtagacagc gaaaaacggc tacgcccgga
    21301 gcgcaccagc atccgttcac cttccagctg tacggcaatt tccgcgcctt caggcagacc
    21361 acggcagata tcaaagaatt tgcgcgccgg aacggtcgtc gctcctggct cgtgtggctg
    21421 aaccagcgca acgcgtgcca ccatttccat ctcgagatca gtaccggtca gcgacaacgt
    21481 accgtcagca acctgtaaca gcagattacc gagaatcggt agcgtaggac gaccacctaa
    21541 cggcccgctc acctgttgta gcggttttaa taaatgctca cgttctacgg taaatttcat
    21601 aggtttacga tgacaatgtt ctgattaaat ttgaaaaatc ttctttgata tcgtggctct
    21661 cttcacgcaa ctgctcgatc ttacggcagg catgaagcac cgtcgtgtgg tcacgaccac
    21721 caaacgcatc gccaatctcc ggcagactgt ggttagtcag ctctttcgcc agcgccatcg
    21781 ccatctggcg cggacgcgcc accgagcggg atcgacgctt ggaaaggaga tccgcgactt
    21841 tgatcttgta gtactccgcc accgtcttct gaatattgtc gatggtgacc agtttttcct
    21901 gcaatgccag caagtcgcgc agtgcctcac gcacgaagtc gatggtgatc gcccgtccgg
    21961 taaagttggc gttagcaata acgcggttca gcgccccttc cagctcacgt acgttagatc
    22021 gtagacgctt ggcgataaag aacgccactt cgcctggcaa acgaatgtca ttttcgtcgg
    22081 cctttttcat caggatcgcc acgcgggttt ccagctctgg cggttcgatc gccacagtca
    22141 gtccccaacc gaagcgggat ttcaaacgat cctcaacgcc gttgatctct ttcggatagc
    22201 gatccgaggt gagaatgatc tgttgattac cttccagcag ggcgttgaag gtgtggaaaa
    22261 actcttcctg agatcgttct ttattagcaa aaaactgaat atcgtcgatc agcagtgcat
    22321 ctacggaacg gtagtagcgt ttaaactctt cgatcgcgtt gttttgcagg gctttaacca
    22381 tgtcctgaac aaagcgctcg gagtgcatat aaaccacttt ggcattcggc ttgcgcgcca
    22441 taatgccgtt acccaccgca tgcagcaggt gagttttacc cagacccgtg ccgccataaa
    22501 ggaacaatgg gttataggca ccgccagggt tatccgccac ctggcgagcc gccgcgcgcg
    22561 ccagttggtt agatttacct tcaacgaagt tatcaaacgt gtgtttgacg tttacgttag
    22621 aacgataggt cggttctgcc ggggccggga cgttatccca acctgagcgc gtagaaggcg
    22681 cagcacgttg cggctgcgtt tgcgccacct gtgcaggggc cgcgacgttg ctcgtcactg
    22741 ccgcttgtgg cgtttgcgtc accggtttgg tgccgacttc aaaacgcagc tgtggggcat
    22801 ccgctccgca gaaactggtt agcagtccat tgatattatt aaggtacttg tcccgtaccc
    22861 aatcgaggac aaaacggttt ggcgcgtaca gggccagcgt gttatcgctc agttccgcct
    22921 gcaatgggcg tatccacata ctgaattctg tggctggtaa ctcatcctgc aatcgggcaa
    22981 gacactgctg ccaaagcgaa agtgacacgg cggactccac tcgaacaaaa gtcgataatg
    23041 actaaggctg aaacattcat gattgttgac gtacgtcgaa aagaccctgc ttgagggtga
    23101 cgcacgaacc gctgtctgcg gttatatgcc cgatcaagat cctgcaaaac gatcgggacc
    23161 gcggatcata gcctaaactg cgcaagagat cttctgtttc tcacagattt tcccgattta
    23221 tccacaggac tttccagacc tcgctaagtg taaacgatcc tgccgcgagg cgggcacgat
    23281 ttacgccgca tattggaaaa tttaatgacc atagacaaaa attggcttaa tcgatctaat
    23341 aaagatctag gacgatcctt gcgctttacc catcagcccg tataatcctc cacccggcgc
    23401 gccatgctgg ttttcactgg tgtgaggtcg tacattttcc ctgcgaaaag gtgcggaaaa
    23461 gcgcggtaaa taaggaaaga gaattgactc cggagtgtac aattattaca atccggcctc
    23521 tttaatcacc catggcttcg gtgtccatcg tttcattttt cggcggatat ccaataaagc
    23581 cattgaattt attcaagttt aggtagaaat cgccatgaaa cgcacttttc aaccgtctgt
    23641 actgaagcgc aaccgttctc acggcttccg tgctcgtatg gctactaaaa atggtcgtca
    23701 ggttctggca cgtcgtcgtg ctaaaggccg cgctcgtctg accgtttcta agtaataaag
    23761 ctaacccctg agtggttaag ctcgcatttc ccagggagtt acgcttgtta actcccagtc
    23821 aattcacatt cgtcttccag cagccacaac gggctggcac gccgcaaatt accattctcg
    23881 gccgcctgaa ttcgctgggg catccccgta tcggtcttac agtcgccaag aaaaacgttc
    23941 gacgcgccca tgaacgcaat cggattaaac gtctgacgcg tgaaagcttc cgtctgcgcc
    24001 aacatgaact cccggctatg gatttcgtgg tggtggcgaa aaaaggggtt gccgacctcg
    24061 ataaccgtgc tctctcggaa gcgttggaaa aattatggcg ccgccactgt cgcctggctc
    24121 gcgggtcctg atagccctca ttcgggtcta tcaacgcctg attagtccgc tactcgggcc
    24181 gcattgtcgt ttcactccaa cctgttcaag ctacggaatt gaggcattgc gcaggtttgg
    24241 agtgataaaa ggcagttggt tgacggtgaa acgcgtatta aaatgccacc ctttacaccc
    24301 tggtggtgac gatcccgtcc cgcccggacc atttgatacc agagaacact aacgatggat
    24361 tcgcaacgca atcttttagt catcgctttg ctgttcgtgt ctttcatgat ctggcaagcc
    24421 tgggagcagg ataaaaaccc gcaacctcag gcccaacaga ccacgcagac aacgaccacc
    24481 gcagcgggta gcgccgccga ccagggcgta ccggccagtg gccaggggaa actgatctcg
    24541 gttaagaccg acgtgcttga tctgaccatc aacacccgtg gtggtgatgt tgagcaagct
    24601 ctgctgcctg cttacccgaa agagctgaac tctacccagc cgttccagct gctggaaact
    24661 tcaccgcagt ttatttatca ggcacagagc ggtctgaccg gtcgtgatgg cccggataac
    24721 ccggctaacg gcccgcgtcc gctgtataac gttgaaaaag acgcttatgt gctggctgaa
    24781 ggtcaaaacg aactgcaggt gccgatgacg tataccgacg cggcaggcaa cacgtttacc
    24841 aaaacgtttg tcctgaaacg tggtgattac gctgtcaacg tcaactacaa cgtgcagaac
    24901 gctggcgaga aaccgctgga aatctccacc tttggtcagt tgaagcaatc catcactctg
    24961 ccaccgcatc tcgataccgg aagcagcaac ttcgcactgc acaccttccg cggcgcggcg
    25021 tactccacgc ctgacgagaa gtacgagaaa tacaagttcg ataccattgc cgataacgaa
    25081 aacctgaaca tctcttcgaa aggtggttgg gtggcaatgc tgcaacagta tttcgcgacg
    25141 gcgtggatcc cgcataacga cggtaccaac aacttctata ccgctaatct gggtaacggc
    25201 atcgccgcta tcggctataa atctcagccg gtactggttc agcctggtca gactggcgcg
    25261 atgaacagca ccctgtgggt tggcccggaa atccaggaca aaatggcagc tgttgctccg
    25321 cacctggatc tgaccgttga ttacggttgg ttgtggttca tctctcagcc gctgttcaaa
    25381 ctgctgaaat ggatccatag ctttgtgggt aactggggct tctccattat catcatcacc
    25441 tttatcgttc gtggcatcat gtacccgctg accaaagcgc agtacacctc catggcgaag
    25501 atgcgtatgc tgcagccgaa gattcaggca atgcgtgagc gtctgggcga tgacaaacag
    25561 cgtatcagcc aggaaatgat ggcgctgtac aaagctgaga aggttaaccc gctgggcggc
    25621 tgcttcccgc tgctgatcca gatgccaatc ttcctggcgt tgtactacat gctgatgggt
    25681 tccgttgaac tgcgtcaggc accgtttgca ctgtggatcc acgacctgtc ggcacaggac
    25741 ccgtactaca tcctgccgat cctgatgggc gtaacgatgt tcttcattca gaagatgtcg
    25801 ccgaccacag tgaccgaccc gatgcagcag aagatcatga cctttatgcc ggtcatcttc
    25861 accgtgttct tcctgtggtt cccgtcaggt ctggtgctgt actatatcgt cagcaacctg
    25921 gtaaccatta ttcagcagca gctgatttac cgtggtctgg aaaaacgtgg cctgcatagc
    25981 cgcgagaaga aaaaatcctg attcggtgag ttttcgctaa aataagggcg gtcagttgac
    26041 cgcctttttt cttttcgtag ggtggataag caccgcgcat ccgccacaca aagcaacagg
    26101 aacatcatga gcgataatga cactatcgta gcccaggcca cgcctccggg acgtggcggc
    26161 gttggcatcc tgcgcatctc cggcctaaaa gcccgtgaag ttgccgaaac cgtgctgggt
    26221 aaactgccta agccgcgcta cgccgattat cttccgttta aagacgccga cggcagcgtg
    26281 ctcgatcagg ggattgcgct atggttccct ggcccgaact cgttcaccgg cgaagatgtg
    26341 ctggaactgc agggtcatgg cggtccggtg atcctcgacc tgctgttaaa acgcattctg
    26401 accattcccg gcctgcggat tgctcgccct ggtgagtttt ccgaacgcgc gtttcttaac
    26461 gataaactcg acttagccca ggccgaggcg attgccgatc ttatcgacgc cagttcggaa
    26521 caggcggccc gttcggcact taactcgctg caaggcgcat tctccgcacg ggtcaaccat
    26581 ctggtggaag ccctcaccca cttgcgcatt tacgtcgaag cggcaattga tttccctgat
    26641 gaagagatcg atttcctctc cgacggcaaa attgaagccc agcttaatga cgtgattgcc
    26701 gatctcgatg cagtgcgtgc tgaagcacgt cagggtagtt tgttgcgcga agggatgaag
    26761 gtggtgattg ccggacgtcc taacgccggt aaatcgagcc tgttaaacgc gctggcgggc
    26821 cgtgaagcag caatcgtaac tgatatcgcc ggaaccacac gtgacgtgct gcgtgagcat
    26881 atccacattg acggaatgcc gctgcatatc atcgataccg ctgggctacg tgaagccagt
    26941 gacgaagtgg aacgtattgg tatcgagcgc gcgtggcagg aaattgaaca ggccgaccgc
    27001 gtgctgttta tggtcgatgg caccacaaca gacgccgtgg atccggcaga gatctggccg
    27061 gaatttatcg cccgtctgcc agcgaaacta ccgatcaccg tggtgcgcaa taaagccgat
    27121 atcaccggcg aaacgctggg aatgagtgaa gtgaacggtc acgcgttaat tcgtctctcg
    27181 gcaaggacag gtgaaggcgt ggaggtgctg cgtaaccatc tcaaacagag catgggcttt
    27241 gacaccaaca tggaaggcgg cttcctggcg cgtcgtcgcc acctacaggc gctggaacag
    27301 gcagcggagc atttgcagca aggtaaagcg caactgttag gtgcctgggc gggtgaactg
    27361 ctggcggaag agttgcgcct ggcacagcag aacttaagcg aaatcaccgg ggaatttacc
    27421 tctgatgatt tgctggggcg gattttctcc agcttctgta ttggtaagta accgcgctta
    27481 cgaagccgta ttctgactgt cagatgcggc ttcgcttcat tgttagcact cctgttattc
    27541 ctcaaccctt tttttaaaca ttaaaattct tacgtaattt ataatcttta aaaaaagcat
    27601 ttaatattgc tccc
//
LOCUS       DAJTVK010000046        27504 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000046 DAJTVK010000000
VERSION     DAJTVK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.046"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(36..119)
                     /locus_tag="OO372_002004"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="OO372_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="OO372_002005"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="OO372_002005"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR6341501.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="OO372_002006"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="OO372_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR6341502.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="OO372_002007"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="OO372_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6341503.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="OO372_002008"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="OO372_002008"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6341504.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="OO372_002009"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="OO372_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6341505.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="OO372_002010"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="OO372_002010"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6341506.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="OO372_002011"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="OO372_002011"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6341507.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="OO372_002012"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="OO372_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="OO372_002013"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="OO372_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6341508.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="OO372_002014"
     CDS             complement(12425..12664)
                     /locus_tag="OO372_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341509.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="OO372_002015"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="OO372_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6341510.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="OO372_002016"
     CDS             13041..13328
                     /locus_tag="OO372_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6341511.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13998..14141
                     /locus_tag="OO372_002017"
     CDS             13998..14141
                     /locus_tag="OO372_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6341512.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14154..14363
                     /gene="cspE"
                     /locus_tag="OO372_002018"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14154..14363
                     /gene="cspE"
                     /locus_tag="OO372_002018"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6341513.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14529..15338
                     /gene="rlmA"
                     /locus_tag="OO372_002019"
     CDS             14529..15338
                     /gene="rlmA"
                     /locus_tag="OO372_002019"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6341514.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="OO372_002020"
     CDS             complement(15335..15901)
                     /gene="mntP"
                     /locus_tag="OO372_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6341515.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16330..16788)
                     /locus_tag="OO372_002021"
     CDS             complement(16330..16788)
                     /locus_tag="OO372_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR6341516.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="OO372_002022"
     CDS             complement(16843..17694)
                     /gene="manZ"
                     /locus_tag="OO372_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6341517.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(17707..18507)
                     /gene="manY"
                     /locus_tag="OO372_002023"
     CDS             complement(17707..18507)
                     /gene="manY"
                     /locus_tag="OO372_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6341518.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(18570..19541)
                     /gene="manX"
                     /locus_tag="OO372_002024"
     CDS             complement(18570..19541)
                     /gene="manX"
                     /locus_tag="OO372_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6341519.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            20004..21554
                     /gene="yoaE"
                     /locus_tag="OO372_002025"
     CDS             20004..21554
                     /gene="yoaE"
                     /locus_tag="OO372_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6341520.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(21558..23156)
                     /locus_tag="OO372_002026"
     CDS             complement(21558..23156)
                     /locus_tag="OO372_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6341521.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="OO372_002027"
     CDS             complement(23287..24651)
                     /gene="sdaA"
                     /locus_tag="OO372_002027"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6341522.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(24835..25413)
                     /locus_tag="OO372_002028"
     CDS             complement(24835..25413)
                     /locus_tag="OO372_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6341523.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="OO372_002029"
     CDS             complement(25417..26778)
                     /gene="pabB"
                     /locus_tag="OO372_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6341524.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            26852..27031
                     /locus_tag="OO372_002030"
     CDS             26852..27031
                     /locus_tag="OO372_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6341525.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(27151..27474)
                     /locus_tag="OO372_002031"
     CDS             complement(27151..27474)
                     /locus_tag="OO372_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6341526.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7081 a         6775 c         6943 g         6705 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    13501 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    13561 tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct
    13621 ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa
    13681 aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta
    13741 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
    13801 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    13861 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    13921 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
    13981 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    14041 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    14101 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    14161 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    14221 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    14281 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    14341 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    14401 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    14461 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    14521 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    14581 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    14641 tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    14701 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    14761 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    14821 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
    14881 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
    14941 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    15001 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    15061 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    15121 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    15181 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct
    15241 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    15301 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    15361 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt ttcccaataa
    15421 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
    15481 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
    15541 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
    15601 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    15661 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
    15721 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    15781 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
    15841 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    15901 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    15961 catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    16021 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    16081 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    16141 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
    16201 gattttcatt tggatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
    16261 ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
    16321 attcataact tattgagttg aaacgagaag cttataaatt ttttccagat cgtcgatatt
    16381 tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga
    16441 caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt
    16501 ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag
    16561 tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt tatagataag
    16621 aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat
    16681 tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc
    16741 caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga
    16801 taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc
    16861 gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac
    16921 ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat
    16981 taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat
    17041 gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac
    17101 aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc
    17161 tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag
    17221 acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc
    17281 gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc
    17341 tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat
    17401 ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc
    17461 cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc
    17521 gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc
    17581 ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg
    17641 aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct
    17701 cacctgttag tccagttcgt tatcgagatc gttgttacca gctgcctgag caggcgcacc
    17761 ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccaat
    17821 cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa
    17881 gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac
    17941 gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat
    18001 gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg
    18061 cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt
    18121 atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt
    18181 cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg
    18241 atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac
    18301 tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat
    18361 accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg
    18421 aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag
    18481 cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt
    18541 gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc
    18601 attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc
    18661 agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt
    18721 ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca
    18781 acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat
    18841 ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc
    18901 ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac ttcatcacta
    18961 acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga
    19021 atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt
    19081 tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca
    19141 accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc cagcgcaacc
    19201 agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg
    19261 ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg
    19321 aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt
    19381 gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg
    19441 atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct
    19501 gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat
    19561 cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc
    19621 agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa
    19681 ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta
    19741 aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc
    19801 tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg
    19861 gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta
    19921 tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc
    19981 tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc
    20041 tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca
    20101 ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac
    20161 tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca
    20221 aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg
    20281 gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg
    20341 atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg
    20401 tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc
    20461 atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca
    20521 aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc
    20581 tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt
    20641 acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc
    20701 gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc
    20761 tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat aacccgatgc
    20821 cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc
    20881 ttgcgtcgcg ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc tgggttgacg
    20941 ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc
    21001 cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg
    21061 tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg
    21121 tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct
    21181 ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc
    21241 tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg
    21301 gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg
    21361 ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa
    21421 atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca
    21481 ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg
    21541 atgaagatga gtaagcatta acgtaacggc ataatgggcg tgatatgtcc attatgccgg
    21601 gcgggcggcg gttggctgcc cgccagccat ttgggaaaat cacgtagcgg catcggctgt
    21661 gcgtacaaat acccttgcaa aatatgcacg ccatgacggc gcaaatactt cgcctgttct
    21721 tgcgtttcca cgccctccgc caccagttca atattcagtc tttgccccag cgcgatgatg
    21781 atatcggtca ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa tgacttatca
    21841 attttcagca tgtcaggacg taatgtttca agccaggaaa atgaactgtt gccggtgccg
    21901 aagtcatcaa tcgccagttt gacgttttta cgatgtagct cgcgggcaat ccgataatca
    21961 acatccagta aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg
    22021 ctaaaccagt actgattgag atcttttatc agcacaccac ggcgaaaatg gctgggtgcg
    22081 acgttaatgc caacatgaaa ctgactactc atcgggaaaa catggcgctg acgaatggtt
    22141 tctgccatca catagcgggt cagtggcaca attaaatgat gttcttccgc gataggaata
    22201 aacacatccg gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa aatctctaca
    22261 ccaatacatt gctggctgcg cgcattaagc aaaggctgac agaacaattc gaactcatgt
    22321 tgcgccaggc ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag
    22381 gcgatgtagc ccaccagtaa acttagcagc accgccagcg gtaattgtgt tggaagatag
    22441 tgccatgcca gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc
    22501 gaagaaacct gatagttttc attgtcttca ggttgcggaa gggaatctac cagcccatta
    22561 ccatagagca aatgccgttt gtccaccgtc aggctggcgc tactgatttg cggcagttgt
    22621 ggctcaagga gcattgccgt cagtaagtcg atgttaatca tctccatgac cccagcattg
    22681 ctactgcccg ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat cagggcacgg
    22741 tcgatcgtca ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat gtccacgacg
    22801 ggcacattgc gataaccaaa aatgctggag caatagagtg ttccgtcttg caccaggcca
    22861 atggatcgca cagtttggag ttttgccgcc tgtttacgta atggcaaatg ggcgacagag
    22921 cagggcagac caatcagcgg aagcaagact tcgctacctg cctgtagggg aagcagtact
    22981 ttatctaatt cctctacagc gtgattggcg aattgtacta cccgttgttg atttaagtta
    23041 cgctgtgaaa taaatcgcac gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta
    23101 caaacaatca ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg
    23161 gccaccctgt aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa
    23221 tattacagca gacgagaaag cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa
    23281 gaagtattag tcacactgga ctttgattgc cagaccaccg cgtgaggttt cgcggtattt
    23341 ggcgttcatg tccttaccag tttcgtacat cgtttcgatg accttatcca gcgagacgcg
    23401 cggtgcactg gtgcggcgca gagccatccg tgcggcgtta atcgccttca cagaggcaat
    23461 ggcattacgc tcaatgcacg gcacctgaac ctgccctgca accgggtcgc aggttaaacc
    23521 aaggttgtgt tccatgccaa tttccgccgc cacgcaaacc tgttccgggc taccgcccag
    23581 cagttctgca agacccgcag cagccattga acaggcaaca cccacttcgc cctggcaacc
    23641 aacttccgca ccggaaatag aggcgttcat tttatacaat gcaccaatcg cgcccgctgc
    23701 cataaagtaa cgggtataga tgtccgggct gaccgattca ataaagtggt catagtaagc
    23761 cagcactgcc ggaacgatac cgcaggcacc gttggttggc gcagttacca cacgaccacc
    23821 ggcggcgttt tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa tgacattcat
    23881 cggatcgtta gacagtttat cgctggaaac taacatccgg cgcagggcag acgcacgacg
    23941 tggcacgcgc agcgggcctg gcagtacacc ttcggtgttc atcccgcgat cgatacatgc
    24001 ctgcatggtt tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc
    24061 cagttcgttc tgcatagcga gaccagacag cgaatagccg gtttcattac agtacgcgag
    24121 cagttcggtg gcagatttga acggatacgg cacgctcact tcgttggcag catcctgacc
    24181 aaagtgttct tcatcgacga taaaaccgcc gccgatggaa taataagttt tgctgtagac
    24241 gacttcatcg ccgttatagg cgtggatttg cataccgttt tcatgcagcg gcaggttgcc
    24301 gttatgaaaa cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag
    24361 cagcagacgt tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt cgatatccac
    24421 ggtggcaggt tcgttacctg caagacccat aataatggcg atatcggtgt ggtggccttt
    24481 acccgtcagc gacagtgaac cataaacgtc cacggcaacg cgagtaacgc tatccagtaa
    24541 gcctttttcg accagatcat cgacgaactg tttacctgcc ttcataggcc ctacggtatg
    24601 ggaagatgag ggaccaatcc ccaccttaaa catgtcgaat agactaatca cgataatact
    24661 cctgacaagg tgactggact tccagtaacg aactaataac agcgcatagt gtaagaggga
    24721 acgcggcgac tggcttaact attcacatga attaaactaa tcaaaaaccg cgatttaact
    24781 aatattgcga tgattgtctc aagtagtgtt ttgtagatgt aaagataagt atagtcaggg
    24841 tttcacacca atttgcagcg ccagctcacg aattatgcct gcggtcattc cccatacaaa
    24901 atactgttcg taccaggaca gccatacccg atgtgaatcg ccacggcggt agatatctaa
    24961 agggtgataa cgacccagat gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac
    25021 ttcatcttca ctggcgcgat acggcagatc gggcgggata atgccgacca ctggggttac
    25081 ctgatagcca gttacgctat cgacgggcgg cagcacgccg ataacttcaa cggcggaagg
    25141 cggtatagcg acctcttctt cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt
    25201 gtcatcgact gcacctccag ggaatgccac ttgtccagcg tgtttacgca gatgaattga
    25261 acgctgagtc agcaacaacc ccggttgcgg tcgacggacg atggggatta acacggcagc
    25321 ctgacgatga tttagggttt cccggttaat ttgcgggcgc aaaagttgaa agcgcgataa
    25381 aaaatcatca agcatcaggc tacggtattc cacgtcttac ttctccagtt ggcgtaatat
    25441 cttattaact ttatcaaaag tttcctgata ttccgcttct tcctggctat cggcgacaat
    25501 tccaccgccc gcagagcagt atatttgtcc gttaatggca gtcagagtgc ggatagtaat
    25561 gctggtatcc atgttgccgc aaaagctcaa atagccaatg ctgccgcacc aggcattacg
    25621 tcgctgcggt tccagttcgt cgataatttc catagcccgt actttcggag ccccggttat
    25681 tgagccacca ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt ctggtagttg
    25741 tgccgttatg gtgctgacca gatgatgcac ggcagggaag ggttccacca cgaacagctc
    25801 tggtactttt accgaacctg ctacggcaac acgaccgata tcattacgca ttaaatcgac
    25861 aatcatcaga ttttcggcac gatctttcgc tgagttcgcc agtttttctg cttgtttgct
    25921 atcttcctga ggatcgggca ggcgtggtag cgtgccttta atagggcggg tctggatttc
    25981 actattatca caaagaataa accgctctgg cgaaaggctt aaaattgcac cctgttcaag
    26041 acgtaaaaaa gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga atgcctgcca
    26101 ttcatcgcca gaataggtcg catgaaaacg ctgggcgaga tttacctgat agcaatcacc
    26161 gctgtgcaga tattcctgta cctggcgaaa tttttcgccg tactgctcgc gggtcatatt
    26221 ggattgccag tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct ggctttccag
    26281 ccaggcccga cgggcattga catcattatg actcagcaaa gaaactgtat gacgctggtg
    26341 gtcgacaatg agcgcccaat cgtagatacc cactgccata tccggcagaa cgatatcttg
    26401 ttccgcaatt tctggcagtg actcaaaacg gcggcccaga tcgtagccaa acaaccccag
    26461 tgcgccgccc tgaaatggca aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag
    26521 cacctgctgg agcacctgta gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact
    26581 aacaacggtt tctttaccta aagtggttaa agtgcaaatc ggatcggcga ccacaatatc
    26641 aaagcggcta tacggatgat cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg
    26701 gcttaagcgg gagaaataaa attcagcggc gtcctgacgc cagggtaaag taatcacagc
    26761 gggagataac gtcttcatca gtcctgactc tactggctat gtgccgggta gcatgaaata
    26821 attagcgcca acaatttagc aggagttaac aatgtttgca ggtttacctt cactcaccca
    26881 tgaacagcag caaaaagctg tcgagcggat ccaggaactg atggcacaag ggatgagcag
    26941 cggccaggca atcgcgttgg tggcagaaga gctgcgtgcc aaccattccg gcgagcgaat
    27001 cgtggcgcgt tttgaggatg aagacgagta acacactaat ccgtattgat ttcgttatac
    27061 aagccgatga ttatttgatt gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc
    27121 ctgatccatt tatgctgatg taaattttac tcagaccagt gcttttagtt cttcctgcat
    27181 atatgttgaa aagtattcag gatttttaat cacagagcgt tcacctgact ccattttttt
    27241 tgcccatcca accatttttt ccgtaaaccc agggttccag ccttcctggt cagctcgcgc
    27301 cgtaatatca gcggcactcg caggtactcc cagttctttt aaatatttaa agattccctt
    27361 cgccgtgctt tcattcatcg aacttggtgt tggcgctgat acatccatgg caccaatgaa
    27421 atccagggct ttatctatta cagcaggcat attcttatcc ttaaaattaa ccatgttatc
    27481 gataaggcta tatatgacct taat
//
LOCUS       DAJTVK010000047        27108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000047 DAJTVK010000000
VERSION     DAJTVK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.047"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..924
                     /locus_tag="OO372_002032"
     CDS             <1..924
                     /locus_tag="OO372_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6341527.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OO372_002033"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="OO372_002033"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6341528.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="OO372_002034"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="OO372_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6341529.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="OO372_002035"
     CDS             3885..4280
                     /locus_tag="OO372_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6341530.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="OO372_002036"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="OO372_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6341531.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="yraP"
                     /locus_tag="OO372_002037"
     CDS             4900..5475
                     /gene="yraP"
                     /locus_tag="OO372_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6341532.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="OO372_002038"
     CDS             complement(5589..6629)
                     /locus_tag="OO372_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6341533.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="OO372_002039"
     CDS             complement(6702..7337)
                     /locus_tag="OO372_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6341534.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="OO372_002040"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="OO372_002040"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6341535.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="OO372_002041"
     CDS             complement(7963..8406)
                     /locus_tag="OO372_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6341536.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="OO372_002042"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="OO372_002042"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6341537.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="OO372_002043"
     CDS             complement(8746..9249)
                     /locus_tag="OO372_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6341538.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="OO372_002044"
     CDS             complement(9243..9767)
                     /locus_tag="OO372_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6341539.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="OO372_002045"
     CDS             9976..10971
                     /locus_tag="OO372_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6341540.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="OO372_002046"
     CDS             10980..11858
                     /locus_tag="OO372_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6341541.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="OO372_002047"
     CDS             12064..13071
                     /locus_tag="OO372_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR6341542.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OO372_002048"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="OO372_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6341543.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OO372_002049"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="OO372_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6341544.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OO372_002050"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="OO372_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6341545.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OO372_002051"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="OO372_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6341546.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OO372_002052"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="OO372_002052"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6341547.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OO372_002053"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="OO372_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6341548.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OO372_002054"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="OO372_002054"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6341549.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OO372_002055"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="OO372_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6341550.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OO372_002056"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="OO372_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6341551.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OO372_002057"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="OO372_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6341552.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OO372_002058"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="OO372_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6341553.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="OO372_002059"
     tRNA            complement(26829..26905)
                     /locus_tag="OO372_002059"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
BASE COUNT         6421 a         7518 c         6812 g         6357 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcgg tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga tgagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct cgatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatagc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaggctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc ccccttaa
//
LOCUS       DAJTVK010000048        26765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000048 DAJTVK010000000
VERSION     DAJTVK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.048"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            110..382
                     /locus_tag="OO372_002060"
     CDS             110..382
                     /locus_tag="OO372_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6341554.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            475..558
                     /locus_tag="OO372_002061"
     CDS             475..558
                     /locus_tag="OO372_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341555.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            658..1479
                     /locus_tag="OO372_002062"
     CDS             658..1479
                     /locus_tag="OO372_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6341556.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OO372_002063"
     CDS             complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="OO372_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6341557.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OO372_002064"
     CDS             complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="OO372_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6341558.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2611..3645
                     /gene="tqsA"
                     /locus_tag="OO372_002065"
     CDS             2611..3645
                     /gene="tqsA"
                     /locus_tag="OO372_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6341559.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OO372_002066"
     CDS             complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="OO372_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6341560.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OO372_002067"
     CDS             complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="OO372_002067"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6341561.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7125..8069
                     /locus_tag="OO372_002068"
     CDS             7125..8069
                     /locus_tag="OO372_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6341562.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8255..9637
                     /locus_tag="OO372_002069"
     CDS             8255..9637
                     /locus_tag="OO372_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6341563.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9674..10396
                     /gene="folM"
                     /locus_tag="OO372_002070"
     CDS             9674..10396
                     /gene="folM"
                     /locus_tag="OO372_002070"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6341564.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10393..10728)
                     /locus_tag="OO372_002071"
     CDS             complement(10393..10728)
                     /locus_tag="OO372_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6341565.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10857..11576
                     /gene="rstA"
                     /locus_tag="OO372_002072"
     CDS             10857..11576
                     /gene="rstA"
                     /locus_tag="OO372_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6341566.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11580..12881
                     /gene="rstB"
                     /locus_tag="OO372_002073"
     CDS             11580..12881
                     /gene="rstB"
                     /locus_tag="OO372_002073"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6341567.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12957..13886
                     /gene="tus"
                     /locus_tag="OO372_002074"
     CDS             12957..13886
                     /gene="tus"
                     /locus_tag="OO372_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6341568.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="OO372_002075"
     CDS             complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="OO372_002075"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6341569.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="OO372_002076"
     CDS             complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="OO372_002076"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6341570.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17274..18449
                     /gene="manA"
                     /locus_tag="OO372_002077"
     CDS             17274..18449
                     /gene="manA"
                     /locus_tag="OO372_002077"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6341571.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18550..20058
                     /locus_tag="OO372_002078"
     CDS             18550..20058
                     /locus_tag="OO372_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6341572.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="OO372_002079"
     CDS             complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="OO372_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6341573.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="OO372_002080"
     CDS             complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="OO372_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR6341574.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="OO372_002081"
     CDS             complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="OO372_002081"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6341575.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="OO372_002082"
     CDS             complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="OO372_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6341576.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="OO372_002083"
     CDS             complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="OO372_002083"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6341577.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6715 a         6937 c         6432 g         6681 t
ORIGIN      
        1 ccattcccag ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa
       61 ttgagggtat gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
      121 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
      181 agcagcgccg gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa
      241 agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag cccctgaaca
      301 aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac cggcaaaacc
      361 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
      421 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
      481 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
      541 cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt
      601 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
      661 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
      721 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat
      781 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
      841 gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg
      901 gcaggacact gtttattgac acctccaaag ggtaaagccg ataaagcagt ggcgctgcgt
      961 tttgtgtcaa ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat
     1021 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
     1081 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacaccgttg
     1141 ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc tggccgtaaa
     1201 gtaactcagg cgggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
     1261 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
     1321 gacagcggtt cgcctctgat gttgcatacc aatgacggct ggcaattaat tggggtgcaa
     1381 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
     1441 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgactg
     1501 tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
     1561 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
     1621 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
     1681 acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat
     1741 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
     1801 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
     1861 ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt
     1921 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
     1981 ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta aggcgatctt
     2041 tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
     2101 gccgccattt ccctcactga cgctcgccca tttcattgac agcgtaccgg taatttctgt
     2161 agcaatagcc aggcctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
     2221 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata
     2281 gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
     2341 gcaagatagt ggatgaggta cgaagatggg taaatgttga aaataacgtc ctgaacaaat
     2401 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
     2461 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
     2521 acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
     2581 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc
     2641 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
     2701 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
     2761 gtgcaacaca tggtccgctg gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc
     2821 atcatcgtga tggcgatggt gttgctatta gcttatctgg gttccgcgct caacgagttg
     2881 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg
     2941 ttgttgcaac gcgtagggat tgacgtctca gttgaccagc tggcgcatta cattgatccg
     3001 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
     3061 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
     3121 aaatttcagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca acgtgcaatt
     3181 gacagtgttt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc
     3241 gcctgggcga tgctcgccgc actcgatgtt cgcttcgctt ttgtctgggg attgctggcc
     3301 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct
     3361 caggtactgg tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg
     3421 attaatctgg tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt
     3481 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
     3541 atgctgattt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
     3601 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
     3661 ccgtcagggt tacagagctt tcaggattgc atccacgcta gctttggcgt caccaaacag
     3721 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
     3781 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
     3841 aataggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
     3901 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
     3961 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
     4021 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
     4081 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
     4141 ggagtttttc agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc
     4201 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
     4261 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag
     4321 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
     4381 gccggagtac gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc
     4441 gactaaatgc cagccgaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg
     4501 caggccgacg ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
     4561 cgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca
     4621 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
     4681 gaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
     4741 atagctgtta aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac
     4801 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
     4861 aatgaccatc gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc
     4921 gattaacgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
     4981 tttcgaaaga ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
     5041 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
     5101 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
     5161 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
     5221 aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca
     5281 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
     5341 gcgcaacgct tgccagccaa ccgaaaagga tgattgccag cgccatcaac gcgtatttac
     5401 gccacggtga gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg
     5461 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
     5521 tcacggtcac gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct
     5581 ctttgcacaa cagtttcagc agattaacga ggttagtacc gtaaagctgc gaggattgcg
     5641 tcggcagacg gcccggaaga tcggtataac caatcacttt gacaccattt tccgtagtga
     5701 agatttcacc cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa
     5761 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
     5821 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
     5881 gttccatttc cgctttaatg aacgcgtccg acatcacttt ggcatagcca tcgccactgc
     5941 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
     6001 tcacttccgg acgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
     6061 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
     6121 cggccgcagt aatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg
     6181 cgcgataacc ggcgatgttc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga
     6241 tacgcggcac agaatccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
     6301 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
     6361 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
     6421 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
     6481 gacccgcgcc gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt
     6541 ttggcgttgc tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca
     6601 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
     6661 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
     6721 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
     6781 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
     6841 ctgataattg tcttcgacgg ccgggtaaaa cgagacatcg ccccggcacg attcactact
     6901 taacattaaa ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg
     6961 ttttttaagc atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg
     7021 aaaaatgacg cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat
     7081 aacaatacca gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca
     7141 ccctcctggc gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac
     7201 tgacaccgga gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt
     7261 ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg
     7321 cctcttttta tgttgtcgac acttctgatt ttggtaacag cggtaactgg cgtgtggtcg
     7381 ctgacctcta taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg
     7441 gtgttgtcga actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg
     7501 tccagggctt ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag
     7561 cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga tgccaaccaa ggcggcaacc
     7621 agcgtattac tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg
     7681 atgtgatccc ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg
     7741 cgaagaaagt tgagatccta ggtgttgcga ctaccgcatc accaagttct gaagtcggtc
     7801 gtttctttga aacccagtca tcaaaaggcg ggcgttacac cgtcatgctc ccggatggca
     7861 ctaaagtcga agaactgaac aaagcgaccg cagcgatgat ggtcccgttc gacagcatca
     7921 aattctctgg caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg
     7981 cgaagaaagg tgctaagtat taccacatca cccgccagtg gcaggaacgt ggtaataacc
     8041 tgaccgtcag cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat
     8101 gccgcctttc gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc
     8161 ttcgtaaaat cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt
     8221 attcttctta tcacgtttta atcactggat atcgatgaaa aagaaactgg gactgagcgc
     8281 actcaccgcg ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa
     8341 tatggcggca gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg
     8401 cattttattg ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg
     8461 cggtatcttt acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg
     8521 gggatactgg ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc
     8581 gttaagcttt tttacggaca cgccggaatt gcgcctgttc ggcgatggta atacctggca
     8641 atcgatcgtc ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt
     8701 gcaaaccgct gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct
     8761 gtttgttgtg ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg
     8821 acttgcactt ggcgtacccg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg
     8881 ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga
     8941 tgttggcaaa gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac
     9001 gctgctttcg ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat
     9061 ggccggtctg atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt
     9121 gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt
     9181 cctggctgcc actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc
     9241 gccatccgcc tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg
     9301 gctgaccggt tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc
     9361 ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt
     9421 aggtgtcggt gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt
     9481 gctgttatcc gttgttctgt atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac
     9541 gcatacacat gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat
     9601 tgcctcagtt ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc
     9661 aaaggagata acgatgggta aagcccagcc cttgccaata ttaattactg gcggaggtcg
     9721 tcgcatcggc ctcgccctcg cctggcattt cattaatcaa aagcaaccgg tgattgtcag
     9781 ctatcggacg cactatccag ccattgatgg actgattaag gcaggtgcgc agtgtattca
     9841 ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagccc
     9901 ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc
     9961 gccactggcc gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct
    10021 caaccatgcg ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca
    10081 ctttaccgat tatgtggtgg agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa
    10141 agcggcactg gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt
    10201 gaactctatt gctccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca
    10261 acaggcgctg aataaatcac tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct
    10321 ggtcgattac ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg
    10381 tcgtcatctg cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc
    10441 ccaacacgaa acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca
    10501 cagcgacacc aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc
    10561 gcgtaaggct tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt
    10621 cgggaaaagt ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat
    10681 caacagcact accagcgcac cgagcgcggc tttgattacc agccccattt ttttacctta
    10741 acacttccat aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga
    10801 tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga
    10861 acactatcgt atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg
    10921 caaaacatga tatgcaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt
    10981 tgcgagaaaa tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga
    11041 ccatttgtcg tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg
    11101 atagcgatat gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa
    11161 cgacgccccc tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag
    11221 ccacactgac caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg
    11281 gcacgttgac catcgatccc atcaaccgcg tagtcaccct ggctaacact gaaatctcgc
    11341 tctcgacagc tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg
    11401 accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg
    11461 tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc
    11521 gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga
    11581 tgaaaaaact gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc
    11641 tgctggttgg gctggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg
    11701 atttgatgaa cagttcactg tatctgatgc gcagcgaatt gcgtgagatc cccccacacg
    11761 actggggtaa gacgctgaaa gagatggatt taaatctctc tttcgatctg cgtgtcgagc
    11821 cactgagtaa ataccatctt gatgatattt ccatgcaccg actgcgtggc ggcgaaattg
    11881 tcgccctgga cgatcagtac acgtttttgc agcgtatccc gcgcagccac tacgtgctgg
    11941 cagttggtcc tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc
    12001 tgatcacttt tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact
    12061 ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg
    12121 aacgtatcca ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga
    12181 tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc
    12241 acgaactgcg aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga
    12301 gcgccgccga atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag
    12361 agctgctgac ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag
    12421 acctgccgtt gtggctgtca acgcatctgg cagacattca ggcagtaacg cccgataaaa
    12481 cggtacggat taaaacgctc gcgcaaggcc attatgcggc gttggatatg cgcttaatgg
    12541 agcgcgtgct ggataatttg ctcaataacg ccctgcggta ctgccattca acggttgaaa
    12601 ccagcctgct actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga
    12661 ttgccccaga aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg
    12721 atcgctcaac cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa
    12781 tgggcggtac ggttaattgt gacaccagcg aactgggtgg tgctcgcttc tcgtttagct
    12841 ggccgttatg gcataacatc ccgcaattta cctctgcctg acactacgcg cacgatggtc
    12901 aagtcaccac cactgtgcta taaaataagt atgttgtaac taaagtggtt aatattatgg
    12961 cgcgttacga tctcgtagac cgactcaaca ctacctttcg ccagatggaa caagagctgg
    13021 ctgcatttgc cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc
    13081 cggaggtaaa aaaagaggat gagcataatc cgcttaatcg tattgaggta aaacaacatc
    13141 tcggcaacga cgcgcagtcg ctggcgttgc gtcatttccg ccatttattt attcaacaac
    13201 agtccgaaaa tcgcagcagc aaggccgctg tccgtctgcc tggcgtgttg tgttaccagg
    13261 tcgataacct ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga
    13321 ccacgttcga gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat
    13381 gggtgcatcg tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc
    13441 tgcacgaccc cgccacttta cgctttggtt gggctaataa acatatcatt aagaatttgc
    13501 atcgtgatga agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac
    13561 cgtggacgcg cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc
    13621 tgccacagaa cgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg
    13681 tctggtacaa aggagatcaa aaacaagtcc aacacgcctg ccctacaccg ctgattgcac
    13741 tgattaatcg ggataatggc gcgggcgtgc cggacgttgg tgagttgtta aattacgatg
    13801 ccgacaatgt gcagcaccgt tataaaccac aggcgcagcc gcttcgtttg atcattccac
    13861 ggctgcacct gtatgttgcg gattaacgcc cggctttcat actgccgacc atctgttctg
    13921 gccgtaccca gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt
    13981 ttaaggtcag cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa
    14041 tgtgggtgtt aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct
    14101 cacgattcgg ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca
    14161 gcaagcgcac cgattgcagg aaattgtgga tcaccattgg acggaagacg ttcagttcaa
    14221 agttaccgga agcgccaccc atgttgatcg ccacgtcgtt ccccattacc tgacagcaga
    14281 gcatggttaa tgcttcacac tgggttgggt tcactttccc cggcatgatt gagctgcccg
    14341 gctcattttc cgggattgag atttcaccaa ttccgcagcg cgggccagag gccagccagc
    14401 ggacatcatt ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct
    14461 gaaccagggc atcacaggtc gcaagcgctt caaatttgtt cggcgcggta acaaacggtg
    14521 cacaggtaat gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta
    14581 gtccagtacc caccgctgta ccgccgagag ccagttccgc tacgtgaggc aggctgtatt
    14641 cgatatgttt gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg
    14701 ttagcggcgt ggcgtcctgc aagtgggttc gaccgatttt gacgatatcg gcaaatgcac
    14761 gcgatttttc actcagtgtc tgtgtcaggg ttttaacctg cggaatgagt tgcttgcgca
    14821 gcgccagcag cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt
    14881 tgttcacgtc gtcgttaggg tgaactttac gttccatccc gcgcacgccg ccgagtaatt
    14941 cactggcccg gttagccagc acttcgttca tgttcatgtt actttgcgtg ccggagccgg
    15001 tctgccagat agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg
    15061 cctgccgaat ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg
    15121 ccgctgcgcg cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg
    15181 aaatgcggaa atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga
    15241 catcaatcgc ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tgctcctcac
    15301 ctgattaatt ttttctttct gttttgcctt cgttaagcaa cttttagctc acttattatt
    15361 tactatttga taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc
    15421 ggctctgttt atttcacaca gcgagtgcac tgtgtgagtt gtatctgctg gaagaagtca
    15481 tttcctttat catccacaag gataaacgcc gggaaatctt ccacttcaat tttccagatg
    15541 gcttccattc ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc
    15601 aatacagcgg ccggaccacc gatactgcca aggtagaagc cgccgtgttt tttacaggca
    15661 tccgtcacct gctggctgcg gttgcctttc gccagcatga tcatacttcc gccctgcgct
    15721 tgcagttgat cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag
    15781 gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttaatgtac
    15841 tgcggcagcc cttcaccgtt atccatccgc tctttcagtt tggcgtgagc aatatcacga
    15901 ccgacgataa tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt
    15961 gcgaggatct ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct
    16021 ttacgcagct cttcagggat atatttacct ggattatgtt ccagtttttc gatccagatc
    16081 ccctgacggt tgatcttcgc tttgatatta cggtcagcag agcaggaaac acccatacca
    16141 accggacagg atgcgccgtg acgtggcagg cgaatcacgc gtatgtcgtg agcgaagtat
    16201 ttaccaccaa actgcgcacc cagaccgaga ttttgcgctt cgatcagcaa ttctttttcc
    16261 agttccacat cgcggaatgc ctgaccgtgc tcattccctt ccgttggcag ttcgtcatag
    16321 tatttagcgg aagccagttt cactgtttta aggttagttt ctgcagaagt tccaccaatt
    16381 acgaacgcaa tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc
    16441 aggtaatttt tcagtttccc cggcgttagt aacgctttgg tttcctgata gagatacgtc
    16501 ttgtttgccg aaccaccacc tttggcgata cagaggaatt tgtactcgtc gccatcaacg
    16561 gcataaagat cgatctgcgc tggcagattg gtgccggtat tcacctcttt atacatatcc
    16621 agcggcgcgt tttgcgagta gcgcaaatta tcttcgatat aagtgttata gacaccgcgc
    16681 gccagcgccg cttcatcaca gccaccggtc catacacgct gccctttttt accaacaata
    16741 atcgcagtgc cggtatcctg acaggttggc agaacacctt tcgccgcgat gtcggagttg
    16801 cgcaggaatt gcagcgccac atatttatca ttttcgctgg cttccgggtc acgcagaatg
    16861 tcggccacct gttgttggtg agccggacgc agcataaatg acgcatcatg aaacgcctga
    16921 cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca
    16981 gatacgctaa cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga
    17041 aaaggagcct gataatgaaa gggtttgttt gacattgttc tctcacttac tgcctggttt
    17101 ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacac
    17161 acttttttaa caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta
    17221 ggattcttgc tttaatagtg ggattaattt ccacattaaa acagggattg atcatgcaaa
    17281 aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt
    17341 atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga
    17401 aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg
    17461 agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt
    17521 tcctgttcaa agtattatgc gcagcacagc cgctctccat tcaggttcat ccaaacaaac
    17581 gcaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg
    17641 agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc
    17701 ttgcgatgaa cgcgtttcgt gagttttccg agattgtctc cctactccag ccagtcgcag
    17761 gtgcacatcc ggcaattgct cactttttac aacagcctaa tgccgaacgt ttaagcgaac
    17821 tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa
    17881 aatcggcgct cgacagtcag cagggtgaac cgtggcaaac gattcgttta atttctgaat
    17941 tttacccgga agacagtggc ctgttctccc cgctgttact gaatgtggtg aaattgaatc
    18001 ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc
    18061 tggaagtgat ggcgaactcc gataacgtgc tgcgtgcggg tctgacgcct aagtacattg
    18121 atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga
    18181 cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct
    18241 tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt
    18301 tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac
    18361 cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc
    18421 gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct
    18481 aagcttatta aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg
    18541 ataatcatta tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc
    18601 tggacaggcg gcgcatggta tacaggcaag aagattgaaa cccatctcga agacatggtc
    18661 gcgcaggcga acgcgcaact caaactgaca gctcctgaat ccaacctgga agtgagttat
    18721 caaaactatc atcgcggcgt attcagcagc cagttgcaac tgttggtgaa acccattgcc
    18781 gggaaagaaa atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat
    18841 catggtccct tcccgcttgc ccagcttaaa aaactgaacc tgatcccgtc gatggcatca
    18901 attcaaacca cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt
    18961 gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt
    19021 tcgctcaagc cactgaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag
    19081 ttccagttaa atgctgacag agacggcaaa gccatctccc tttccgggga ggcgcaaagt
    19141 ggtcggatag acgcagttaa cgaatacaac cagaaagtgc agttgacctt taataatctg
    19201 aaaaccgacg gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg
    19261 tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg
    19321 gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat
    19381 tactcgctaa acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta
    19441 aaagtcggcc agattgatgg tgaagcctgg catcagttta gccagcaata taacgcgcaa
    19501 actcaggcgc tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa
    19561 gtgacggaag ccttctttag cgccctgccg ctgatgttga aaggcgatcc ggtgattact
    19621 atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc
    19681 ctgaaagatc cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt
    19741 tcggttaaat ctctggatgc gaaactgacc attccggtgg atatggcaac tgagtttatg
    19801 actcaggtag cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag
    19861 caagttgaag gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat
    19921 accatcacca ccagcctgca atatactaac ggtcagataa cgttaaacgg gcagaaaatg
    19981 ccactggaag atttcgttgg tatgtttgcg atgccagctc ttaacgttcc ggctgtaccg
    20041 gcaattccgc agcagtaatt cacggccaaa acgccggaga attttcactt cggcgttttc
    20101 atctgaaaac aaaccagaga ttgcctgatg cgctacgctt atcaggccta ccttttcctg
    20161 caacactttg aatttatgag tttttgtagg ctggataagg cgtttacacc gcatccggca
    20221 taaaaaacgc gcactttgtc aacaatctga aacgccggag gtttttctct ccggcgttat
    20281 ttctgacttc agcataaagt catactgaat actcattttc acatcccagt aattcaggcg
    20341 atcgcccagc gtactgccat cgcgcttttc ccgcccttta tcggcagcaa gaaagaaatt
    20401 catcccttgt aacggccctt cgctgacccg atagttcata ccacctaaga tacccgcgcc
    20461 ttcaatccgc acatctcgtt tactggattc atagccgcga gtcacaatct gtgcaaatgt
    20521 cagtgtaaat tgcggcgtta aacgatagtt gacgcccaat tgccaggact gcattccttc
    20581 acggttacga tcaagactta atgaaaaggg atagccaatg tcggtatcca ccacggcacc
    20641 attcattgca tggcgcaaat aaccaccact gccaaatacg cttccccacg gcgcattcca
    20701 ggtcaattgt ccactaacca acgccgtttc attgggctgg ctggtattac gacttaaacc
    20761 ttcgagctgc gcgtaaaagc cgagcaactc ccctttcaac gtataattcg ggtcgagcgg
    20821 tatgcttttc tcgagaatac tttgtatctg attggaaaca tctttctgta ccgatgcccc
    20881 cagagaatag cgactggtat catcatgcca actggcggct aaaaaagaac gtgcgcgatg
    20941 tttatcgtat tttcccgtct ggctgttata caccccttca tcacgaatac cggttgttga
    21001 atcagtccat aaaaatactc gtgaacgcca cattccccac agatcaatat ttggtgttaa
    21061 agcccaataa cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg aactgccagc
    21121 cattaacgga atacgataag gtaagctacc ataactataa tccgtcccga agcgacctga
    21181 tttaagcagg ctattttcac tgggttttat ttttactgcc cccagggcaa agccaaaact
    21241 tttatcacca tcaagatacc accgggtact catatcagca cgagcaccta atttataaac
    21301 ataatatgcc ccgccttcaa cgccaataat attggaataa taaccactat tgaaatcgag
    21361 caatcctccc tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg gcttatccgc
    21421 cctgcgcaat tcgtttttca gtctgatgcg tagtcccgcc tcatcgtcag ccagttgcgc
    21481 cgcataagca gagataaggc cagaggcagc cgtcaggcaa ataacggcca tggccactat
    21541 ttttctcata gttgcacctt tgatgttctg attattttta ttgattatta attagtgata
    21601 tcgctgatta attgctgctg cactttttta cgattatcaa tttcaaccac gatttctttg
    21661 aattttttat ccgtaagcgg ataaaaccag ataataacga atgccagtag cataaatccg
    21721 caaggtacta aggcaattga tgtgcggatg cccataataa cttccggcgt ttgcacctga
    21781 ttggcaatat atccgcttaa ccccaaaata aaggcaggaa ttgaacctcc gattgcctga
    21841 ccgcatttac gggtaaatga gaatagtgaa taggtgagcc cttcaattcg cacgccggtc
    21901 aggtattcac cgtattctac ggtatcagct tccagtgccc acatcactgt catagtaacg
    21961 ccctgaccaa ttgaagcgat ggccaacgca accaacgcca ccggcagtga ccagacggaa
    22021 acccagaaga atagcagata accgcaggtt cccagcaaag cgccaatcag gaaggtattc
    22081 tttttaccga tcctcgcgac catccccggc acaagcggtg ccgatgccac agtaccaacc
    22141 aggttttgca ccagtaccag cacagtgaac agcccggtat catttaacac atagcgcacg
    22201 tagaacaacg acgaggcgct gaccgcaaag gtcgaaatca gcacacacag cgcaccgatg
    22261 cacaacataa acagcgggcg attccgtttc agggtttgca aactgatctt caatgacggc
    22321 tgcgcaacga tacgtaccac attctcacgc gtcgatttga agcagatgaa gtaaagcacc
    22381 attccggcaa tcgccagcac aatcgtccag aaatggtata ccgacaccat ctcttccggg
    22441 ctggagttct taatgctcgg ccctatcaga aatgccaggc agacaaaggt caatgaagcg
    22501 gcaatcccac gagccgcgcc cagacgggcg cgggattgtg gttgttgggt catcgcggta
    22561 gcaagtgaac cataaggaat attcaccagg ctgtagcaaa gcccgaggcc catgtaggtc
    22621 aaatatgcat acactacttt gcggctatgg ctccagtcgg tcggtaccca gaataccagc
    22681 acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa ttttccccag
    22741 cgggtattca cactgtccac cactcgtccg gcaaagacgt cggcgaaggc atcgaatacc
    22801 cgcaccagta acagcatggt gcccgccgca gcggcaccga cgccagcgac gtcggtgtag
    22861 taactcaaca ggaagagcac ccccattgcg aaggcgaagt tattggcgac gtcaccgagg
    22921 ctgtagccga cgatggtgcg ccaggagagt tgttgattca ttgtttgcct ccctgctgcg
    22981 gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg
    23041 gtttgcggtc gcgggtgaag atccccttct tgttaccgcc aacgcgcaat atgccttgcg
    23101 aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa
    23161 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
    23221 acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat
    23281 gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat
    23341 cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc
    23401 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg
    23461 ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac
    23521 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
    23581 ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt
    23641 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
    23701 agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca
    23761 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
    23821 atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt
    23881 catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct
    23941 tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt
    24001 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
    24061 ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac
    24121 gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac
    24181 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt
    24241 agagcattac gctgcgatgg atcccggcat agttaaagaa atcatggaag taagactgct
    24301 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
    24361 cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat
    24421 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca
    24481 ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt
    24541 tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat
    24601 cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc
    24661 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
    24721 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct
    24781 cattaagata aaaatactgg tcaaccttta atctgattag ttaaccaatt ctgtgttgca
    24841 cacagttatc tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac tgacaccgcg
    24901 atcaaaaaac caaaaactgc aatacccaca gcgtgatatt gatcgcatta atctttaaaa
    24961 cggcgttgac agaagccata aaataagcaa ccgaacgttc gttcgagcat attgggaacg
    25021 ataaactctc atgacatatg gctacggtga atatttttgg gggggaattc tccgatcccg
    25081 gctagattgt attttgtaca gggaaattaa agtatgaaac gcgggcacag ttatcaccag
    25141 cccgcgaaat gagaggatca ggatgcggtt aagataccgc caatcatcgc gcgtaatccc
    25201 tgagcaaacg acgcttcacg cgccagatct tccgcttcga tatcggccag tacaccgtag
    25261 gtcagatcca gcagtaaacg tgaagtcatt gacgtgttga tgtctgggct tatctcgcct
    25321 ttttgctggg catcacgcat ccgctgggca acaaattccg tgatcgtcat atgcttattt
    25381 ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc gaccaccagc
    25441 gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat atagtcaaca
    25501 aaatgaatcc ctttaatcgg ttcccggaaa cgagccagcg ccctctcctg gtcctgtaag
    25561 ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta
    25621 atggcgcaag atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa
    25681 atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta
    25741 tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc
    25801 gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatactt ttcactctga
    25861 aattggttaa ccaatagccg tgatttggct gccagttatt tagcagagac gttgatgagt
    25921 ttatcccggt aaacaacacg cacggttgtt taccggtccg ttagtgtatt aattgagctc
    25981 ctgtacccca ccaccggaga cggtgagaat ttgtccgctt acccagctcg cagcaggcga
    26041 gcaaaggaac agcgctgcgt tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt
    26101 gtgctgtaac attttttgtt caatttctgg tgtaataacg gatttcaggg catcggttaa
    26161 tattgccccc ggcgcaatgc catttacccg aatatttttt tcacccaggt caaacgccat
    26221 atttctgacc agatgactgg ctgcagcttt agatgatgca taggaagtca tgtttatatt
    26281 tttattttct gccgccatag aagtgatggt cagaataacg ccaccgccat ttttttccat
    26341 ttctggcgca acaagttgtg acagatggaa aaaagaaaac acattcagtt cataagcacg
    26401 gcgaaaatcc gccattggca tatcaaacgg tttaggtcca ccgccaccgg cgttgttaac
    26461 cagaatatca actttaccca gcttactgat agcaaagtct gccagtgcag agagttcctg
    26521 ttcggaagta atatcacaac ggcaggcaaa tgcctgacca cccagttgtt gaatttcgtc
    26581 tacaacatgg ttagctgcgt cggcgttaat atcactgacc accacagatg cgccagctgt
    26641 cgcgaatgta atggcgattt ctttaccaat acctgcaccc gcacctgtga tgatggcgca
    26701 tggcgcatgg cgcatggcgc atggcgcatg gcgcatggcg catggcgcat ggcgcatttt
    26761 ccgtc
//
LOCUS       DAJTVK010000049        25923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000049 DAJTVK010000000
VERSION     DAJTVK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.049"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            16..486
                     /locus_tag="OO372_002084"
     CDS             16..486
                     /locus_tag="OO372_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR6341578.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            488..907
                     /gene="gspB"
                     /locus_tag="OO372_002085"
     CDS             488..907
                     /gene="gspB"
                     /locus_tag="OO372_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCR6341579.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1145..1456
                     /gene="rpsJ"
                     /locus_tag="OO372_002086"
     CDS             1145..1456
                     /gene="rpsJ"
                     /locus_tag="OO372_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6341580.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1489..2118
                     /gene="rplC"
                     /locus_tag="OO372_002087"
     CDS             1489..2118
                     /gene="rplC"
                     /locus_tag="OO372_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6341581.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2129..2734
                     /gene="rplD"
                     /locus_tag="OO372_002088"
     CDS             2129..2734
                     /gene="rplD"
                     /locus_tag="OO372_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6341582.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2731..3033
                     /gene="rplW"
                     /locus_tag="OO372_002089"
     CDS             2731..3033
                     /gene="rplW"
                     /locus_tag="OO372_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6341583.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3051..3872
                     /gene="rplB"
                     /locus_tag="OO372_002090"
     CDS             3051..3872
                     /gene="rplB"
                     /locus_tag="OO372_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6341584.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3889..4167
                     /gene="rpsS"
                     /locus_tag="OO372_002091"
     CDS             3889..4167
                     /gene="rpsS"
                     /locus_tag="OO372_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6341585.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4182..4514
                     /gene="rplV"
                     /locus_tag="OO372_002092"
     CDS             4182..4514
                     /gene="rplV"
                     /locus_tag="OO372_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6341586.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4532..5233
                     /gene="rpsC"
                     /locus_tag="OO372_002093"
     CDS             4532..5233
                     /gene="rpsC"
                     /locus_tag="OO372_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6341587.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5246..5656
                     /gene="rplP"
                     /locus_tag="OO372_002094"
     CDS             5246..5656
                     /gene="rplP"
                     /locus_tag="OO372_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6341588.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5656..5847
                     /gene="rpmC"
                     /locus_tag="OO372_002095"
     CDS             5656..5847
                     /gene="rpmC"
                     /locus_tag="OO372_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6341589.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5847..6101
                     /gene="rpsQ"
                     /locus_tag="OO372_002096"
     CDS             5847..6101
                     /gene="rpsQ"
                     /locus_tag="OO372_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6341590.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6266..6637
                     /gene="rplN"
                     /locus_tag="OO372_002097"
     CDS             6266..6637
                     /gene="rplN"
                     /locus_tag="OO372_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6341591.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6648..6962
                     /gene="rplX"
                     /locus_tag="OO372_002098"
     CDS             6648..6962
                     /gene="rplX"
                     /locus_tag="OO372_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6341592.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6977..7516
                     /gene="rplE"
                     /locus_tag="OO372_002099"
     CDS             6977..7516
                     /gene="rplE"
                     /locus_tag="OO372_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6341593.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7531..7836
                     /gene="rpsN"
                     /locus_tag="OO372_002100"
     CDS             7531..7836
                     /gene="rpsN"
                     /locus_tag="OO372_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6341594.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7870..8262
                     /gene="rpsH"
                     /locus_tag="OO372_002101"
     CDS             7870..8262
                     /gene="rpsH"
                     /locus_tag="OO372_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6341595.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8275..8808
                     /gene="rplF"
                     /locus_tag="OO372_002102"
     CDS             8275..8808
                     /gene="rplF"
                     /locus_tag="OO372_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6341596.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8818..9171
                     /gene="rplR"
                     /locus_tag="OO372_002103"
     CDS             8818..9171
                     /gene="rplR"
                     /locus_tag="OO372_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6341597.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9186..9689
                     /gene="rpsE"
                     /locus_tag="OO372_002104"
     CDS             9186..9689
                     /gene="rpsE"
                     /locus_tag="OO372_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6341598.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9693..9872
                     /gene="rpmD"
                     /locus_tag="OO372_002105"
     CDS             9693..9872
                     /gene="rpmD"
                     /locus_tag="OO372_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6341599.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9876..10310
                     /gene="rplO"
                     /locus_tag="OO372_002106"
     CDS             9876..10310
                     /gene="rplO"
                     /locus_tag="OO372_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6341600.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10318..11649
                     /gene="secY"
                     /locus_tag="OO372_002107"
     CDS             10318..11649
                     /gene="secY"
                     /locus_tag="OO372_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6341601.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11681..11797
                     /gene="rpmJ"
                     /locus_tag="OO372_002108"
     CDS             11681..11797
                     /gene="rpmJ"
                     /locus_tag="OO372_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6341602.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11944..12300
                     /gene="rpsM"
                     /locus_tag="OO372_002109"
     CDS             11944..12300
                     /gene="rpsM"
                     /locus_tag="OO372_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6341603.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12317..12706
                     /gene="rpsK"
                     /locus_tag="OO372_002110"
     CDS             12317..12706
                     /gene="rpsK"
                     /locus_tag="OO372_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6341604.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12740..13360
                     /gene="rpsD"
                     /locus_tag="OO372_002111"
     CDS             12740..13360
                     /gene="rpsD"
                     /locus_tag="OO372_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6341605.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13386..14375
                     /gene="rpoA"
                     /locus_tag="OO372_002112"
     CDS             13386..14375
                     /gene="rpoA"
                     /locus_tag="OO372_002112"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6341606.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14416..14799
                     /gene="rplQ"
                     /locus_tag="OO372_002113"
     CDS             14416..14799
                     /gene="rplQ"
                     /locus_tag="OO372_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6341607.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14906..15274
                     /locus_tag="OO372_002114"
     CDS             14906..15274
                     /locus_tag="OO372_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR6341608.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15285..15710
                     /gene="zntR"
                     /locus_tag="OO372_002115"
     CDS             15285..15710
                     /gene="zntR"
                     /locus_tag="OO372_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR6341609.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15766..15984
                     /gene="arfA"
                     /locus_tag="OO372_002116"
     CDS             15766..15984
                     /gene="arfA"
                     /locus_tag="OO372_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR6341610.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="OO372_002117"
     CDS             complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="OO372_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR6341611.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="OO372_002118"
     CDS             complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="OO372_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6341612.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="OO372_002119"
     CDS             complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="OO372_002119"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6341613.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="OO372_002120"
     CDS             complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="OO372_002120"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6341614.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20216..20725)
                     /gene="def"
                     /locus_tag="OO372_002121"
     CDS             complement(20216..20725)
                     /gene="def"
                     /locus_tag="OO372_002121"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6341615.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20855..21979
                     /gene="dprA"
                     /locus_tag="OO372_002122"
     CDS             20855..21979
                     /gene="dprA"
                     /locus_tag="OO372_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6341616.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21951..22424
                     /locus_tag="OO372_002123"
     CDS             21951..22424
                     /locus_tag="OO372_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6341617.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22453..22995
                     /locus_tag="OO372_002124"
     CDS             22453..22995
                     /locus_tag="OO372_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR6341618.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23000..23572
                     /gene="tsaC"
                     /locus_tag="OO372_002125"
     CDS             23000..23572
                     /gene="tsaC"
                     /locus_tag="OO372_002125"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6341619.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23577..24395
                     /gene="aroE"
                     /locus_tag="OO372_002126"
     CDS             23577..24395
                     /gene="aroE"
                     /locus_tag="OO372_002126"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6341620.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24392..24649
                     /locus_tag="OO372_002127"
     CDS             24392..24649
                     /locus_tag="OO372_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6341621.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24625..25179)
                     /locus_tag="OO372_002128"
     CDS             complement(24625..25179)
                     /locus_tag="OO372_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6341622.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25652..>25923
                     /locus_tag="OO372_002129"
     rRNA            25652..>25923
                     /locus_tag="OO372_002129"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6612 a         6112 c         6797 g         6402 t
ORIGIN      
        1 gcttctttac aaacattggt agatcagaat ttgccctgga gcgcatcgct taaagtcggg
       61 gacaaaaaat tgcctgttgt ggtcgttcgc gtcggtgagg ctagcgttga tgtcctcgta
      121 ggtcagcaaa cgtggacgct gacacataaa tggtttgagt cggtatggac gggtgattat
      181 cttctgttgt ggaaaatgtc gccggaaggg gagagtacca ttacgcgtga tagcagcgag
      241 gaagagattc tctggctgga aacgatgtta aaccgtgcat tgcatatttc gacagaacct
      301 tcggcggagt ggcgtccctt gttggtagaa aaaataaagc aatttcaaaa aagccaccac
      361 ttgaaaactg acggtgttgt tggcttttca acgttagtac atctctggca ggtggcaggg
      421 gagagtgcct acttatatcg ggatgaagca aatatttccc cggaaacaac agtgaagggg
      481 aaatgacatg tttgaattct atatcgccgc gcgtgagcag aaagaaacag gtcaccctgg
      541 tatcttctcc cgacaaaaac acagcaccat aatatatgtc atttgtttat tgctgatttg
      601 cctgtggttt gcaggaatgg tgttggttgg tgggtatgcc aggcagctat gggcgctttg
      661 gatagtaaaa gcagaagtca ctgtagaggc tgaaacgccc gcttttaaac aatcaacaca
      721 acactatttc ttcaaaaaac agccactccc cgtcgtcgaa tccgtcgagg aggaagatga
      781 cccagacgtc gcggttgaga atgctccctc ttcttctgaa gacgaagaaa atactgtaaa
      841 agaaagcgac gagaaagccg gtttaagaga gcgtgtcaaa aatgcgctga acgaacttga
      901 gagataaccc gaaggctgtt tactttacta gcaatacgct tgcgttcggt ggttaagtat
      961 gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga
     1021 atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc
     1081 atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg
     1141 tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga
     1201 tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc
     1261 gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa
     1321 agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc
     1381 aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca
     1441 gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta
     1501 gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta
     1561 accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc
     1621 taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa
     1681 gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg
     1741 gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgaggtt
     1801 aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc
     1861 tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt
     1921 tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag
     1981 atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc
     2041 aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa
     2101 ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc
     2161 gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt
     2221 tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga
     2281 agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg
     2341 ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga
     2401 ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga
     2461 actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac
     2521 taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg
     2581 tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg acgtacgcga
     2641 tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga
     2701 tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg
     2761 ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc
     2821 gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg
     2881 tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac
     2941 ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc
     3001 cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg
     3061 ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc
     3121 tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta
     3181 acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta
     3241 ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg
     3301 atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca
     3361 tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa
     3421 tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg
     3481 tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga
     3541 tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag
     3601 tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg
     3661 ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtaccg
     3721 cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc
     3781 cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg
     3841 ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct
     3901 ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa
     3961 agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg
     4021 atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtgaccgac
     4081 gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct
     4141 gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact atcgctaaac
     4201 atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta
     4261 agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtactgg
     4321 tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg
     4381 acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc
     4441 cgcgtgcaaa aggtcgtgca gatcgcatcc tgaagcgcac cagccacatc actgtggttg
     4501 tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat
     4561 tcgcttgggt attgtaaaac catggaactc tacctggttt gcgaacacca aagaattcgc
     4621 tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc
     4681 gtctgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac
     4741 tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac gtagaaaaac tgcgtaaggt
     4801 cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga
     4861 actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt
     4921 ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa
     4981 agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga
     5041 aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca
     5101 caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg
     5161 tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa
     5221 aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat
     5281 gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg
     5341 tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc
     5401 tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc
     5461 gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg
     5521 ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct
     5581 ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac
     5641 taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcgttga agagctgaac
     5701 accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc
     5761 cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact
     5821 ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg
     5881 ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc
     5941 cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg
     6001 aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat
     6061 cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat
     6121 acgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg
     6181 ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag
     6241 tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga
     6301 caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta
     6361 cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt
     6421 caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc
     6481 ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga
     6541 gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt
     6601 catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa
     6661 tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag
     6721 ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg gttaagaaac
     6781 atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta
     6841 ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct
     6901 ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt
     6961 aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa
     7021 actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac
     7081 cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc
     7141 agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc
     7201 aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg
     7261 catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtattc gtgacttccg
     7321 tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca
     7381 gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac
     7441 cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt
     7501 cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa
     7561 gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc
     7621 atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact
     7681 ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgcccg
     7741 catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc
     7801 ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag
     7861 taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt
     7921 caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc
     7981 aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct
     8041 gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt
     8101 gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg
     8161 ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc
     8221 caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct
     8281 cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag
     8341 gttattacga tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa
     8401 gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg
     8461 gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc
     8521 ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg
     8581 attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact
     8641 gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ttgataagca ggtgatcggc
     8701 caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttataaagg caagggtgtt
     8761 cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg
     8821 gataagaaat ttgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg
     8881 ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca
     8941 ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa
     9001 ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc
     9061 gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt
     9121 cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt
     9181 gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa
     9241 accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag
     9301 ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag
     9361 cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg
     9421 gcactctgca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg
     9481 cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg
     9541 gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg
     9601 caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta
     9661 aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa
     9721 cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ctgggtctgc
     9781 gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg
     9841 cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg
     9901 ccgaaggctc caaaaaggcg ggtaaacgct tgggtcgtgg tatcggttct ggcctcggta
     9961 aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg
    10021 gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc
    10081 gtaaagcagc gattacagcc gaagttcgtc tgtctgacct ggctaaagta gaaggcggtg
    10141 ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga
    10201 aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta
    10261 aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg
    10321 gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc
    10381 agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc
    10441 cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt
    10501 gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg
    10561 atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg
    10621 ttggcggaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgc
    10681 tacggtactc tggtgctggc aatattccag tcgatcggta ttgctactgg tctgccgaat
    10741 atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt
    10801 gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat tactgaacga
    10861 ggtatcggca acggtatttc aatcattatc ttcgtcggta ttgtcgcggg actcccgcca
    10921 gccattgccc atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg
    10981 ttggttgcag tattagtatt tgcagtgacg ttctttgttg tatttgttga gcgtggtcaa
    11041 cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgttta tgctgcacag
    11101 agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc
    11161 agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac
    11221 tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat
    11281 gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa
    11341 acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa
    11401 acggcgaagt atatcgataa agtaatgacc cgcctgaccc tggttggtgc gctgtatatt
    11461 acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc
    11521 ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa
    11581 actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac
    11641 ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt
    11701 caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg
    11761 cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca
    11821 aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat
    11881 ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc
    11941 atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta
    12001 acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc
    12061 gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa
    12121 gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc
    12181 ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt
    12241 cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa
    12301 tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca
    12361 agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac
    12421 tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc
    12481 tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa
    12541 agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc
    12601 tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc
    12661 gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca
    12721 ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg
    12781 tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat
    12841 tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca
    12901 gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa
    12961 ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct
    13021 ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc
    13081 acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc
    13141 ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg
    13201 cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga
    13261 tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga
    13321 cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg
    13381 acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag
    13441 tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc
    13501 tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg
    13561 ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc
    13621 tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta
    13681 ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg
    13741 gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt
    13801 ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa
    13861 ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc
    13921 ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg
    13981 acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc
    14041 gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc
    14101 agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg
    14161 atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg
    14221 gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat
    14281 ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcctgg
    14341 aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact
    14401 gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc
    14461 catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag
    14521 acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc
    14581 aagactgata gcgttgctaa tcgtcgtctg gcatttgccc gtactcgtga taacgagatc
    14641 gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact
    14701 cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg
    14761 gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaat gtaaaaaaac
    14821 ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat agctgtactc
    14881 tttttgttca tcccctggag tatttatgtg gttacttgac cagtgggcag agcgccatat
    14941 agcagaagcg caagcgaaag gtgagtttga taacctggca ggtagcggcg aaccattgat
    15001 actggatgat gattctcacg tgccaccgga attacgtgcg gggtatcgct tgctgaagaa
    15061 tgccggttgc ttaccgccag aacttgagca acggagagaa gcaattcagc ttctggatat
    15121 tctcaaaggt atccgtcacg atgatccgca atatcaagag gttagccgtc gattgtcatt
    15181 actggaattg aagctgcgac aagctggatt gagtaccgat tttttacgcg gcgattatgc
    15241 tgacaagttg ttggacaaaa tcaacgataa ctagtggagt atgtatgtat cgcattggtg
    15301 agctggcaaa aatggcggaa gtaacacctg acacgattcg ttattacgaa aaacagcaga
    15361 tgatggagca tgaagtgcgt actgaaggcg ggtttcgcct gtataccgaa agcgatctcc
    15421 agcgattgaa atttatccgc catgccagac aactaggttt cagtctggag tcgatccgcg
    15481 agttgctgtc gatccgcatc gatcctgaac accatacctg tcaggagtca aaaggcattg
    15541 tgcaggaaag attgcaggaa gtcgaagcac ggatagccga gttgcagagt atgcagcgtt
    15601 ccttgcaacg ccttaacgat gcctgttgtg ggaccgccca tagcagtgtt tattgctcga
    15661 ttcttgaagc tcttgaacaa ggggcgagtg gcgttaagag tggctgttga ttttttgcac
    15721 tgacaggatt acactcgcgc cgttaaataa ccaactggag tttttatgag ccgatatcag
    15781 catactaaag ggcagataaa ggataatgcg atagaagcat tactacatga tcccttattc
    15841 cgacagcgag tagagaaaaa taagaaaggg aaaggcagtt acatgcgaaa aggaaaacat
    15901 ggcaatcggg ggaactggga ggccagtggc aagaaagtaa atcacttttt taccactggt
    15961 cttctgcttt caggcgcttg ttaagagcgg ttattctgct ctttcagcaa atcacgaatt
    16021 tctgccagta atacttcttc tttagttggt gcaggtgcgg cagctggttc ttcttttttc
    16081 cgattcagtt tgttgattag cttaatcgcc ataaagatgg caaaggccac aatcagaaaa
    16141 tcaaagacgt tttgaatgaa gacaccgtaa tgcatcacaa cagcagggat atccccctgc
    16201 gcatcgcgta gcgtgacagc aaactgttta aagtcgatcc cgccaattaa taagcccaga
    16261 ggtggcatga tgatatcggc aaccagtgaa gatacaatct tcccgaatgc cgcaccgata
    16321 atgacaccca ccgccaaatc caccacgttc ccgcgcatcg caaattcgcg aaattcttta
    16381 ataatgctca tgttattctc cctatgaagc cgacaaccat aagtctaaca aatgttaagc
    16441 cattttcctg ccaggaatga atttaattaa tccttgataa tcaaagggaa ataataaggc
    16501 gtcattagac gccttattaa ttacaagaag aaagggcttg gctggaagag tctttcgacg
    16561 tcggtaataa actttttatc tgtgaggaac ataattacgt gatcgccttg ctcaatgcgc
    16621 agattgtcat tggcaatcat cacgtcgttt ccacgtacca ccgctccaat aatcgttcct
    16681 ggcggtagct tgatttcgtc aatgactctg ccgacaacgc gtgaggtgct ttcatcaccg
    16741 tgagcaacgg cttcaatagc ttctgctacg ccgcggcgca atgaggaaac accaacaata
    16801 tctgcttttc gcacatggct aagcaacgca gaaatagttg cttgttgtgg tgaaattgca
    16861 atatcgatca cgctcccctg aaccagatcc acataagcgc gacgctggat caataccatc
    16921 acctttttcg cacccatacg tttggcaagc atggcggaca tgatattggc ctcgtcatcg
    16981 ttggtgacgg caataaacag atcaacttga tcgatatgtt cttcggccag cagttcttga
    17041 tccgacgcat caccaaaaaa gacgatcgta ttctgcaact tttccgccag ttcggcagcg
    17101 cgctgctgat tacgttcgat gagtttaacg ctgtaatctt tttccagacg acgcgccagc
    17161 cctgcaccga tattaccgcc accaaccagc atgatccgct tatacggttt ttccagtcgc
    17221 tgtaattcac tcatcaccgc gcggatatgc tgtgaagcgg caataaagaa cacttcatca
    17281 ccagcttcaa caatggtcga accttgtgga cgaatggggc gatcgtggcg gaaaattgct
    17341 gccacacgag tatcgatatg tggcatatgt tcgcgcatgg tcgaaagtgc attaccaatc
    17401 agcgggccgc cataataggc tttaactaca gcgaggctga ctttaccctc agcgaagttc
    17461 accacctgca atgcgcctgg atactcaatc agtcggtaaa tattatcgat aaccaactgc
    17521 tctggtgcga tcagatgatc aatcggtaca gcatctgaat gaaatagctt atcggcatcg
    17581 cgtacgtagt ctggtgagcg gatgcgagcg atgcgattag gggtgttgaa aagtgagtag
    17641 gctacctggc aggcaaccat attggtttca tctgaactgg ttacagcaac cagcatatcg
    17701 gcgtcgtcgg cacctgcctc ccgcaatacg cgtggatgag agccatgccc ctgcacgact
    17761 cgcaggtcaa atttatcctg caaggtccgc agacgctcac cgttggtatc gacaacagta
    17821 atatcgttgt tctcgccaac caggttttcc gccagtgtgc cgccaacctg gccggcaccc
    17881 agaatgataa ttttcatcag tctcgacccg ttatctcatc actttttgat tagcttagcg
    17941 taaaagaagc cgtcgccctc ttcggcacca ggcagatttt gtttacccgg ttgctctggt
    18001 gttcctgttt cgcaaagttc ggcatcagcg gtacgttgca aaaaagcttt aatctgcagg
    18061 ctattctctt ccggtaacac cgaacaggtg gcatagacca gggttccacc ggattttaaa
    18121 tgcggccaaa tggcgtcgag aatttcagac tgcaattgcg cgagttccgg gatatcgcga
    18181 tcgcgacgta accatttaat atccggatgg cgacgaatca cccccgttgc tgaacaaggc
    18241 gcatctaata aaatgcgatc aaactgttgc tcgccacacc attgggaagg gtaacggcca
    18301 tcaccttgtt tcacggtcgc cttcatacca aggcgtttta aattgtcgtg aacgcgagag
    18361 aggcgctgtt cgtcaatatc aaccgcaaca acctgcgctt ctggtgccac ctcaaggata
    18421 tgcgttgttt taccgccggg ggcggcacaa agatccagaa tgtgttcacc gttttgtggc
    18481 gcaagccagg tcatgcaacc ttgtgctgat gcatcctgaa cggtaaccca tccgtcttca
    18541 aaaccaggta gcgcatgaac aggtgcaggt gtttccagcc gtacagcatc agggtaatcc
    18601 gcatgcggga aacctttcat tcctgcttca tccaacaatg caagccagct gtcgcgggaa
    18661 tgatgcgtac gattaacgcg cagccacatt ggcggacgct ggttattggc ttcgacgatg
    18721 gattgccact gctctggata cgctttttgc agacgcttca gcaaccagga aggatgcaga
    18781 taacgtgcat cactggtatt aaactcggct aataactctt cttgctgacg ctggaactgg
    18841 cgtaatacac cgttaatcaa ccctttaagt tgcggacgct taattgcgat agcgccttca
    18901 accgtttcag ccagcgcagc atgaggtgga atgcgggtat aaagcagttg atacaaacca
    18961 accataatca ggtaatgcac agtgcgctgt ttgccggtca tcggacgggc cattaactta
    19021 ttaatcagcc agtctaactg cgaaagcgta cgcagtacgc caaagcacaa ctcttgaaga
    19081 agtgctttgt ctttatcaga aactttttgc tggagcggtg gcagaatgtt gcttaatgat
    19141 tgcccttgct cgacgacttg ttcaacggcc tgggccgcca tgctacgtaa attacgttgt
    19201 tttttcataa gtataaaaat gcccggtaag accgggctta gaagagtgga ctatcaggcc
    19261 agacggttgc ccggaacaaa ccattcccga cgagaattca ggaggtcttg cgcgctcatc
    19321 gctttcttac ccgcaggttg taacgagagc aggttcagga tgccatcacc agtcgcaacc
    19381 tgaatgcctt gtttgttggc ttcaaggatc gttcctggtg cagcgttggt tgccgtatca
    19441 atgaccgacg ctttccagac tttaaccggt tgtccttcaa tttccagcca gctcattggc
    19501 catgggttga aagcgcgaat gcagcgttca agctgcgctg ccgaaagcga ccagtcaata
    19561 cgcgcttctt ctttactcaa cttctcggcg taagtgacaa gagtttcgtc ctgaacttct
    19621 ggtttcgccg tgccgtctgc tagttgtttc aacgtggtga ttagcccttg tgggccaagc
    19681 tctgccagct tgtcgtacag cgtaccactg gtatcttctg cagtaatcgg gcaggagagc
    19741 ttatagagca tatcaccggt gtctaaaccg acatccattt gcataatggt cacaccagtt
    19801 tctgcatcac ccgcccatag tgagcgttgg attggtgcag caccgcgcca gcgtggcagc
    19861 agtgaaccat gaacgttgat acagccaaga cgcggcatct ccagcactgc tttaggcaga
    19921 attaagccat aggcgacgac gaccataaca tcagcctgca gatcggcgac cagttgctgg
    19981 ctttcttgtg gacgcaggga aacaggttga aaaacgggca gacctttttc ctcagccaga
    20041 actttaaccg ggctgggcat cagtttttta ccgcgtcctg ccggtcggtc tggctgggtg
    20101 aacacgccaa cgacgttatg accagaagac aacagcgcgt cgagatgacg cgctgcaaag
    20161 tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca cgttagttct tatccttaag
    20221 cccgggcttt cagacgatct agtttttcaa ctttctgacg aatacgttgt tgtttcagcg
    20281 gtgacagata atccataaac agtttgccga ccaggtgatc catctcatgc tgaatacaga
    20341 tggctaacag cccctctgct tccagttcaa atggtttacc gtcgcggtca agggcgcgaa
    20401 ttttaacttt ctctgcgcgc ggcactaaag cacgttgttc agggatcgac aggcaacctt
    20461 cttcaatgcc tgtttcgccg cttttttcta aaagctccgg attgattaac accagccgtt
    20521 cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg atggatatca acctgggttg
    20581 ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa catatcatcg acgatacgct
    20641 gtatttctgc attcacttct tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa
    20701 tatgtaacac ttgcaaaact gacataaatc tccagagatg tgttcagggg ttagaaagat
    20761 tatttcttct attctagaca aatccccctc tgattgacag catcactgac caatcgcaaa
    20821 gattgctaag gctgcttatg gcagggagat aaggatggtc gatacagaaa tttggctgcg
    20881 tttaatgagt atcagcagct tgtacggcga tgatatggtc cgtatagctc actggctggc
    20941 aaaacagtcg caaattgatg cggttggatt gcagcaaaca gggcttacat tgcggcaggc
    21001 acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc tcactttgtt ggttggagca
    21061 acccaaccat catttaatcc ctgcggacag cgaattttat cctcctcaac ttctggtgac
    21121 gacagattac cccggcgcac tgtttgttga aggagaactg cacgcgctgc attcatttca
    21181 gcttgccgta gtggggagtc gggcgcattc atggtatggc gagcgatggg gacggttatt
    21241 ttgcgaaact ctggcgacgc gtggagtgac aattacgagt ggactggcgc gtggaatcga
    21301 tggtgtggcg cataaagctg ccttacaggt aaatggcgtc agcattgctg tattggggaa
    21361 tggacttaat accattcatc cccgccgcca tgcccgactg gctgccagtc tgcttgaaca
    21421 tggcggagct ctcgtctcgg aatttcccct cgatgttcca ccccttgctt acaatttccc
    21481 acgaagaaat cgcattatca gtggtctaag taaaggtgta ctggtggtgg aagcggcttt
    21541 gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag cagaggcgtg aagtttttgc
    21601 cttgccaggt ccaataggga atccgggaag cgaagggcct cactggttaa taaaacaagg
    21661 tgcgattctt gtgacggaac cggaagaaat tctggaaaac ttgcaatttg gattgcactg
    21721 gttgccagac gcccctgaaa attcatttta ttcaccagat cagcaagacg tggcattgcc
    21781 atttcctgag ctcctggcta acgtaggaga tgaggtaaca cctgttgacg tcgtcgctga
    21841 acgtgccggc caacctgtgc cagaggtagt tactcaacta ctcgaactgg agttagcagg
    21901 atggatcgca gctgtacccg gcggctatgt ccgattgagg agggcatgcc atgttcgacg
    21961 tactaatgta tttgtttgaa acctatattc acactgaagc tgagttgcgt gtggatcaag
    22021 acaaacttga acaggatctt accgacgcag gttttgagcg agaagatatc tacaatgccc
    22081 tgctatggct ggaaaaactt gctgattatc aggaagggtt ggcagaaccg atgcaactgg
    22141 cctcggatcc tctctccatg cgtatttata caccggaaga gtgtgaacga ctggatgcca
    22201 gctgccgtgg gtttctgctt ttccttgagc agattcaggt gctcaacctt gaaactcgtg
    22261 aaatggtgat agagcgagtg ctcgcgctgg ataacgcaga gttcgaactg gatgatctga
    22321 aatgggtgat cctgatggtg ttgttcaaca tcccggggtg cgaaaatgcg taccagcaaa
    22381 tggaagaatt actctttgaa gtgaatgaag gtatgctgca ttaatcattt ctttaattca
    22441 gcataagttg ttatggcaaa atcagcactg ttcacggtgc gtaataatga gtcctgccca
    22501 aagtgcgggg ctgaactggt tattcgatcc gggaaacacg gtccgtttct tggatgctca
    22561 cagtatccgg cgtgtgacta cgtccgtctt ctgaaatctt cagcggatgg acatattgtc
    22621 aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa atctggtatt acgccaggga
    22681 cgctttggta tgtttattgg ttgcagtaac taccctgaat gcgaacatac cgaacttatc
    22741 gataaaccgg acgaaacagc aattacatgc ccccaatgtc ggacgggcca tctggtccag
    22801 cgccgctccc gttatggcaa aacatttcac tcctgtgatc gctacccgga gtgtcaattt
    22861 gccattaact tcaaaccaat agctggagaa tgccctgagt gtcattatcc gctactcatc
    22921 gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg tggaaagccg
    22981 gtttcggcgg aataataacg tgaataataa cctgcaagga gacgctatcg cagctgcgat
    23041 agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt tcggtgttgg
    23101 gtgcgatcct gatagcgaaa cagcagtgat gagactgttg gagttaaaac agcgtccggt
    23161 tgataagggg ctgattttaa tcgcggcaaa ttacgagcag cttaaaccct atattgatga
    23221 caccatgctg actgatgcgc agcgtgaaac cattttttcc cgctggccag gtcctgtcac
    23281 ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg acgggccgct ttgattcgct
    23341 tgctgtacga gtcaccgacc atccgttggt ggttgctttg tgccaggctt atggtaaacc
    23401 gctggtttct accagtgcca acttgagtgg attgccacct tgtcgaacag tagacgaagt
    23461 tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg ggcgtttaaa
    23521 tccttcagaa atccgcgatg ccctgacggg tgaactgttt cgacaggggt aacataatgg
    23581 aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc attcatcagc
    23641 aatttgctca gcaactgaat attgaacatc cctatgggcg cgtgttggca cccatcaatg
    23701 atttcatcaa cacactgaac gctttctttc gtgctggtgg taaaggtgcg aatgtgacgg
    23761 tgccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg gcagcgttgg
    23821 ctggtgcagt taataccctc aagcggttag aagatggacg cctgctgggt gacaataccg
    23881 atggtgtagg cttgttaagc gatctggaac gtctgtcttt tatccgccct ggtttacgta
    23941 ttctgcttat cggcgctggt ggagcatctc gcggcgtact actgccactt ctttccctag
    24001 actgtgcggt gacaatcact aatcggacgg tatcccgcgc ggaagagttg gctaaattgt
    24061 ttgcgcacac tggcagtatt caggcgttgg gtatggacga actggaaggt catgagtttg
    24121 atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg atcccgtcat
    24181 cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga aaaactcctt
    24241 ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta ggaatgctgg
    24301 tggcacaggc ggctcatgcc ttccttcttt ggcatggcgt tctgcctgat gtagaaccgg
    24361 tcatcaaact attgcaacag gaattgtcag cgtgaatcag gccatccagt ttccggatag
    24421 ggaagagtgg gacgagaata aaagatgtgt atgttttcct gctctcgtga atggtatgca
    24481 actgacatgt gcgatctctg gcgagagtct ggcgtatcgc tttactggag gtacgccaga
    24541 acagtggtta gcgagttttc gtcagcatcg ctgggacctg gaagaagaag cggaaaactt
    24601 aattcaggaa caaagtgaag atgatcaagg ttgggtctgg ttaccttgat ccagatattc
    24661 gtccttccat ttcacgtagt tatttgcgga atagcgtaac ccagccttct cttcatcact
    24721 taacgggcgg atctgtttga cgggactacc gagatacaga tatccgctct ccagccgttt
    24781 attttgtggg accaggctac ccgcaccaat catcacatca tcttctacta ttgcgccatc
    24841 aagtaaaatt gagcccatcc caaccaaaac tcgattgcca atggtgcagc cgtggagcat
    24901 caccttgtga ccaacagtga catcttcgcc aatggttaat gggttgccat ctgggttgta
    24961 cgaggattta tgagtgacat gcaacatact gccatcctgg atattggtgc gtgctccgat
    25021 ctgtacataa tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc
    25081 cagacgaacg tcaccaatca cgacgctgct atcgtcgatc attacgcgct ggccgatttg
    25141 tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata ctcacctcaa caataaatga
    25201 tttcctaatg actttgggtg cattattagc cttgtgcaag tcttttagta tgcaaaaaag
    25261 caccgttttg tgtgcgaatg cagcaaaaag ggtgaaaaaa caacaaacag aaaaaaagat
    25321 caaaaaagta cttgtgcaaa aaattgggat ccctataatg cgcctccgtt gagacgacaa
    25381 cgtgaaacac ttcacaggat ggtcggaaca acgaagagaa aaaattctga aattcagggt
    25441 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct gaaagccgcg
    25501 tcgcaactgc tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt
    25561 cttaacgtcg caagacgaaa aatgaatacc aagtctcaag agtgaacacg taattcatta
    25621 cgaagtttaa ttctttgagc atcaaacttt taaattgaag agtttgatca tggctcagat
    25681 tgaacgctgg cggcaggcct aacacatgca agtcgaacgg taacaggaag cagcttgctg
    25741 cttcgctgac gagtggcgga cgggtgagta atgtctggga aactgcctga tggaggggga
    25801 taactactgg aaacggtagc taataccgca taatgtcgca agaccaaaga gggggacctt
    25861 cgggcctctt gccatcggat gtgcccagat gggattagct tgttggtggg gtaacggctc
    25921 acc
//
LOCUS       DAJTVK010000050        25774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000050 DAJTVK010000000
VERSION     DAJTVK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.050"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO372_002130"
     CDS             complement(<1..827)
                     /gene="dgt"
                     /locus_tag="OO372_002130"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6341623.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
     gene            911..1609
                     /gene="mtnN"
                     /locus_tag="OO372_002131"
     CDS             911..1609
                     /gene="mtnN"
                     /locus_tag="OO372_002131"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6341624.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            1602..2402
                     /gene="btuF"
                     /locus_tag="OO372_002132"
     CDS             1602..2402
                     /gene="btuF"
                     /locus_tag="OO372_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6341625.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            2440..3063
                     /locus_tag="OO372_002133"
     CDS             2440..3063
                     /locus_tag="OO372_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6341626.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO372_002134"
     CDS             complement(3110..3454)
                     /gene="erpA"
                     /locus_tag="OO372_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6341627.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(3447..3512)
                     /locus_tag="OO372_002135"
     CDS             complement(3447..3512)
                     /locus_tag="OO372_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341628.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO372_002136"
     CDS             complement(3536..4957)
                     /gene="clcA"
                     /locus_tag="OO372_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6341629.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            5182..6462
                     /gene="hemL"
                     /locus_tag="OO372_002137"
     CDS             5182..6462
                     /gene="hemL"
                     /locus_tag="OO372_002137"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6341630.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO372_002138"
     CDS             complement(6620..8602)
                     /gene="fhuB"
                     /locus_tag="OO372_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6341631.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO372_002139"
     CDS             complement(8599..9489)
                     /gene="fhuD"
                     /locus_tag="OO372_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6341632.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO372_002140"
     CDS             complement(9489..10286)
                     /gene="fhuC"
                     /locus_tag="OO372_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6341633.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO372_002141"
     CDS             complement(10337..12526)
                     /gene="fhuA"
                     /locus_tag="OO372_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6341634.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO372_002142"
     CDS             complement(12746..15280)
                     /gene="mrcB"
                     /locus_tag="OO372_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6341635.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO372_002143"
     CDS             complement(15476..17905)
                     /gene="hrpB"
                     /locus_tag="OO372_002143"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6341636.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            17979..18509
                     /gene="thpR"
                     /locus_tag="OO372_002144"
     CDS             17979..18509
                     /gene="thpR"
                     /locus_tag="OO372_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6341637.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            18524..19228
                     /gene="sfsA"
                     /locus_tag="OO372_002145"
     CDS             18524..19228
                     /gene="sfsA"
                     /locus_tag="OO372_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6341638.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            19406..19861
                     /gene="dksA"
                     /locus_tag="OO372_002146"
     CDS             19406..19861
                     /gene="dksA"
                     /locus_tag="OO372_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6341639.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO372_002147"
     CDS             19898..20824
                     /gene="gluQRS"
                     /locus_tag="OO372_002147"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6341640.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            20863..22281
                     /gene="pcnB"
                     /locus_tag="OO372_002148"
     CDS             20863..22281
                     /gene="pcnB"
                     /locus_tag="OO372_002148"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6341641.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            22278..22757
                     /gene="folK"
                     /locus_tag="OO372_002149"
     CDS             22278..22757
                     /gene="folK"
                     /locus_tag="OO372_002149"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6341642.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            23127..23711
                     /locus_tag="OO372_002150"
     CDS             23127..23711
                     /locus_tag="OO372_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6341643.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            23816..24556
                     /locus_tag="OO372_002151"
     CDS             23816..24556
                     /locus_tag="OO372_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6341644.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            24591..25774
                     /gene="htrE"
                     /locus_tag="OO372_002152"
                     /pseudo
     CDS             24591..25774
                     /gene="htrE"
                     /locus_tag="OO372_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT         6330 a         6984 c         6417 g         6043 t
ORIGIN      
        1 tctgccacac aataggagat gtcgtcggca gcttccataa tccacgttaa tggaaaacga
       61 ctgtaaagcg ccaaattaag ttctttacgc aaccgggcaa tataggcttc ttcagaaaga
      121 taataacccg gctttttcat taaatagtga tgtgtctcag gcgtttcgcc acgccaccac
      181 gccggacggg tatattttaa aataccgcca acctgtgccc aagtgagatt catccgcatc
      241 aatgtatgca ccagacgaat gccttgtgca ttcccctcaa aatgacataa gtcctgacga
      301 atcttgcgcc gcagctcgtt aagctgttct tccccgtccc gtaaacgtag tgccgccacg
      361 ctgcagcgat cgtcagtcag aggctggctt tcggcatctt ccgggtgcaa acgttggcga
      421 aaccagtcat ttatcgctgc ttcgccaaaa tgaccaaacg gcggattgcc gatatcgtgc
      481 atcaggcatg acatctcaac aatgctttca aaggggccgg tcagttcatc caggccgtat
      541 gcttccagca atttaagctc tttcagacgg cttaaaattt ctttggcgat gtagcgtccc
      601 acctgctgga cttccatcga gtgggtaaga cgcgtgcgca cggcggcatt gcgctccagt
      661 ggaaaaacct gggtcttttg ttgcagacga cgaattgccg gagagttgat gatacgcccg
      721 cgatcgctct cgaagatccg caggatctca tgttcggttt taacgccctg cggtgaacgg
      781 taacgacgat gccagtttat tttttttcgg aaatcaatct gtgccatgtc ctctcccgcg
      841 tgagaaatac gcttccccgt aagcgcatgg taaactatgc tttcaaatcg ggcttatcgc
      901 gagtaaatct atgaaaatcg gcatcattgg tgcaatggaa gaagaagtta cgctgctgcg
      961 tgacaaaatc gaaaaccgtc aaactatcag tctcggcggt tgcgaaatct ataccggcca
     1021 actgaatgga accgaggttg cgcttctgaa atcgggcatc ggtaaagtcg ctgcggcgct
     1081 gggtgccact ctgctgttgg aacactgcaa gccagatgtg attattaaca ccggttctgc
     1141 cggtggcctg gcaccaacgc tgaaagtggg cgatatcgtt gtctcggacg aagcgcgtta
     1201 tcacgacgcg gatgtcacgg catttggtta tgaatacggt cagttaccag gctgtccggc
     1261 aggctttaaa gctgacgata aactgatcgc tgccgctgag gcctgcattg ccgaactgaa
     1321 tcttaacgct gtacgtggcc tgattgttag cggcgacgct ttcatcaacg gttctgttgg
     1381 tctggcgaaa atccgccaca acttcccaca ggccattgct gtagagatgg aagcgacggc
     1441 aatcgcccat gtctgccaca atttcaacgt cccgtttgtt gtcgtacgcg ccatctccga
     1501 cgtggccgat caacagtctc atcttagctt cgatgagttc ctggctgttg ccgctaaaca
     1561 gtccagcctg atggttgagt cactggtgca gaaactggca catggctaag tcactgttca
     1621 gggcgctggt cgccctgtct tttcttgcgc cactgtggct caacgccgcg ccgcgcgtca
     1681 tcacgctttc tcccgccaac actgaacttg cctttgccgc cgggatcacg ccggttgggg
     1741 tcagcagcta ttccgactat cctccacaag cgcaaaagat tgagcaggtt tccacctggc
     1801 aggggatgaa tctggaacgc attgtcgcgc tgaaacccga tctggtgatt gcctggcgtg
     1861 gaggtaatgc cgagcggcag gttgaccagc tggcttcgct gggaataaaa gtgatgtggg
     1921 tcgatgcgac aagcattgaa caaattgcca atgcgttacg tcaattggcc ccctggagtc
     1981 cgcaaccgga taaggccgaa caagccgcgc aatccctgct ggatcagtac gcgcaattga
     2041 aagcgcaata tgctgataaa cctaaaaaac gtgtttttct gcaattcggc attaatccgc
     2101 catttaccag tggaaaagag tcgattcaaa accaggtact cgaagtttgt ggcggagaaa
     2161 acatctttaa agacagccgg gttccctggc cgcaagttag ccgcgaacag gtgttagcac
     2221 gctcgccaca ggcgattgtc attacaggcg gaccggacca aattcctaaa atcaaacaat
     2281 actggggtga acagctcaaa attcccgtta ttcctctcac gagtgactgg tttgaacgtg
     2341 caagcccacg tattatcctc gctgcacaac agctctgtaa tgcgctttca caggtagatt
     2401 agcgcctgac ctttttaggt gctcagtggg gattgaacaa tgctcgtcta ttggctggat
     2461 atagtcggca cagcggtatt tgccatctcc ggcgttttgt tagccggaaa attgcgtatg
     2521 gacccttttg gtgttctggt actgggcgtg gttaccgctg taggcggcgg gacaattcgc
     2581 gacatggcgc tggatcacgg cccggtattt tgggtgaaag atcccaccga tctggtcgtt
     2641 gcaatggtaa ccagcatgct gaccatcgtg ctggtgcgcc agccaagacg cttaccaaaa
     2701 tggatgttgc cggtgctgga cgccgttggt ctggcggtgt ttgtcggcat tggcgtgaat
     2761 aaagccttta atgcggaagt cggtccgtta atcgcggttt gtatggtcgt cattactggc
     2821 gttggcggcg ggatcattcg tgatgtactg gcccgcgaga tccccatgat tttacgtaca
     2881 gaaatctacg caactgcctg tattatcggc ggtattgtcc acgctacggc ttattacaca
     2941 ttttccgtac cactggaaac agccagtatg atgggcatgg tcgtgacgct attgattcgg
     3001 ctggcggcta ttcgttggca tcttaagcta ccgacgtttg cgctggatga gaatgggcgt
     3061 tgaagagaga agatgcgccg gatagtttat ccggcacatg aacaacagat tagatgctaa
     3121 aggaagaacc gcaaccgcag gtgcttttcg cgttcgggtt ggtcacgatg aaacgagaac
     3181 cttccagtcc ttcggtatag tcaacggaac cgccgaccag atattgcagg ctcatcggat
     3241 caaccaccaa gccaacgccc tgtttttcga tggtcatatc gccttcgttc acctgatcat
     3301 caaaggtgaa accatactgg aagccgctgc aaccgccacc ggtgatatac acgcgtaatt
     3361 tcagattcgg gttatcttcg tcagcgatca ggcttttaac tttgttggct gctgcgtcgg
     3421 taaactccag cggcagtgct acgtcatcac tcatattttg ctccaaacga catcggcaat
     3481 tgggcaaatt ctaacccaat aataatcgcc attatctaat accctggtat ttcgttcaag
     3541 tattctcgct ggctgatgcg gccttgcttc gcgccagttg ctcagcttcc tgttttgcca
     3601 gcgtgcgcgc aagaatcgcc gagtatagcg gtttcccgcc ggtaaattgc gctaatagtg
     3661 ttgcgccaag accggtaata atcattggca aaatgagctg gtagttatcg gtcatctcca
     3721 gaaccagaat gatccccgtt aacggcgcgc gaatagatgc cgccagtaat gcccccattc
     3781 cggcaatagc aaacgtcccc gcctcaaggt gatattgcgg aaacagctca acggcaacca
     3841 ttccgaaagc ggttcccagc acagtaccca gcgccagcat cggggcaaaa ataccgcccg
     3901 gcgcgccgga agagaagcag agtaaggtgg taatgacccg agcgacgaag ataaacacca
     3961 gcattcccat gctgaaattc cccgcggtag cgataggaat caggttaaaa ccgccgcccg
     4021 acgttgctgg tgccacaaac cccagcaatc cacacagacc gccaatcgca ccgcccatca
     4081 gtacccattt ggtaatattg ccgccgtgca cacggtgcag caaatcctgc atccccagca
     4141 cccatttatt aaaaataggg ccgaaaatgc caaaaataat accgaggatc agataaagcc
     4201 acagcgtatt aagcggcgca tcagaaagtt taccgacgtc aatcaacgca acttcatgat
     4261 taaaaatccg gtacataatg gtcgacataa tgacgccaat aaataccgct tttatcgaaa
     4321 ttaacgtata gcgaaactgc ggacgcatct cttcgataat aaacaaaata cccgccagcg
     4381 gcgcgttaaa ggccgcagcc agccccgcag cagcaccggt tgccagcagc gtatggcgag
     4441 cttcgtcacc tttcaggcgg aaaatatcaa gcaccatacg gccaatgtta ccgccgatct
     4501 gcacggttgg cccttcgcgc cccaacacca tgcctccgcc gagtgtcccc agcccgccaa
     4561 agaacttcac cggcaataca cgccaccagc gaacgggacg ttgatcttcc agcgcccctt
     4621 caatttccgg gatccccgaa ccacctgctt ccggcgcgta tttgcgcacc aaaaagtagc
     4681 caaacatcgc cagcaccgcc gaacagagaa aagcgacggt taacagaagc ggataattat
     4741 cagcagtatg taccagcgcc cccatacgtt ggttctgcaa ccaggcgaca cctttgtcaa
     4801 aagcaaccgc tgccagccca acaagcgtgc cgacgactgc cgccataaac aaaatggcta
     4861 acggggtttt atcgcgctca agaagttggc gaatcagttg tctgcgtcgc aggcgcgcgg
     4921 cctgcggtgt ttctaaagag ggagtatcag ttttcatcca atgatcactt attggtcata
     4981 caaataagat gacggcattt tactcgcacg ttccatgaaa atcctttgca aagcgtaatg
     5041 tttcaaatgc acaaaacttt tatactctaa gaaatcagga ttgcaccagt acaagcagcc
     5101 tgatgtttga cgagtattta acttgttatg aataacatag aatagcagcc attcactttt
     5161 tctacgaatc aggaaccctc catgagtaag tctgaaaatc tttacagcgc agcgcgcgag
     5221 ctgatccctg gcggtgtgaa ctcccctgtt cgcgccttta ctggcgtggg cggcactcca
     5281 ctgtttatcg aaaaagcgga cggcgcttat ctgtacgatg ttgatggcaa agcctatatc
     5341 gattatgtcg gttcctgggg gccgatggtg ctgggccata accatccggc aatccgcaat
     5401 gccgtgattg aagccgccga gcgtggttta agctttggtg caccaaccga aatggaagtg
     5461 aaaatggcgc aactggtgac cgaattggtc ccgaccatgg atatggtgcg catggtgaac
     5521 tccggcaccg aggcgacgat gagcgccatc cgtctggccc gtggttttac cggtcgcgac
     5581 aaaatcatta aatttgaagg gtgttaccac ggtcacgctg actgcctgct ggtgaaagcc
     5641 ggttctggcg cactcacgtt aggccagcca aactcgccgg gcgttccggc agatttcgcc
     5701 aaacatacct taacctgtac ttataacgat ctggcttctg tacgcgccgc gtttgagcaa
     5761 tacccgcaag agattgcctg tattatcatc gagccggtgg caggcaatat gaactgtgtt
     5821 ccaccgctgc cagacttcct gccaggtctg cgtgcgctgt gcgacgaatt tggcgcattg
     5881 ctgatcatcg atgaagtaat gaccggcttc cgcgtggcac tggctggcgc acaggattat
     5941 tacggtgtgg aaccggatct cacctgcctg ggcaaaatca tcggcggtgg aatgccggta
     6001 ggcgcattcg gtggtcgtcg tgatgtaatg gatgcgctgg ccccgacggg tccggtctat
     6061 caggcgggta cgctttccgg taacccaatt gcgatggcag ccggtttcgc ctgtctgaat
     6121 gaagtcgcgc aaccgggcgt tcacgaaacg ttggatgagc tgacaacacg tctggcagaa
     6181 ggtctgctgg aagcggcaga agaagccgga attccgctgg tcgttaacca cgttggcggc
     6241 atgttcggta ttttctttac cgacgccgag tccgtgacgt gctatcagga tgtgatggcc
     6301 tgtgacgtgg aacgctttaa gcgtttcttc catatgatgc tggacgaagg tgtttacctg
     6361 gcaccgtcag cgtttgaagc gggctttatg tccgtggcgc acagcatgga agatatcaat
     6421 aacaccatcg atgctgcacg tcgggtgttt gcgaagttgt gatccagcat ggtttgtcgg
     6481 atgcggcgtg aacgccttat ccgacctacg attcggggcc gtttgtaggc ccgataagac
     6541 gcgccagcgt cgcatcgggc atccgttgtc gcgtaaacgc tttatccgtc caggcgtaca
     6601 gggccgttat atggaaaaat taacggctct gctttctcag caaatagata aaatatggcg
     6661 cgccgataaa ggtcgacagc agccccgccg ggatctggaa tggaaacagc accatccgcc
     6721 cacaccagtc agcgaatacc agcagtaaac cacccaccag cgccgaaatt acgatgtgtg
     6781 gcatcgtccg tcgaaagccc atcatccgcg caatatgcgg tgccattaaa ccaacaaaac
     6841 tcaacggtcc aatagtcatc gtcgcggtcg ccgtcaggca agccgctaac agcagcagcg
     6901 caattcgcgt cggcgtcagc gccattccta cggctcgtgc ggtatcacca cccagcggta
     6961 aaatggtcag ccagcggcgg tacagcgggg taatcgccag caaaatcacc atcacaattc
     7021 cggtacgcca gacctgcgca tcggtcgcgt tgtaggtcga accggaaatc caggtcagca
     7081 cttgcgccat tcgcgggtca ccgctcgcct gcaacatcat caaaagcatg gtgaatgcgg
     7141 tacttaacgc catccccgcc agtaacatac ggtgtgggga aaatccaccg cggccggcgg
     7201 cgatcataat gatcaacagc gtcaccgccg ccccgatact gcccgcgggt aacagccagc
     7261 caaaggcatt acccggcacc agaaacagca ttaacaccac gccaaacgcc gcgccagagc
     7321 taatccccag cacttccggg cttgccatcg ggtttccggt cagccgctga ataatacagc
     7381 ccgccaccgc cagcatgacg cccgcaaaca gcgccgccat aattcgcggc cagcgccagg
     7441 gcatcaaatc ctcgagcaac gccccgctcg cccacgtcca gccgtgcgca tcacgaccaa
     7501 acgacagcgc caccaccaca gccatcaaca gcagcacgcc gcccgcgagg gcaaacgcca
     7561 gcacatgttg gcgttcagcc gcgacacgat cgttgacctt catatccggc gcgctaatgc
     7621 tgcgtaaacg cggcaacagc cacagtagca gcggcgcacc gatcaacgca gtgaccgaac
     7681 cggtggacac ttccatccac acgcgagtca gccagaggat gatttgatcg gaaagccaga
     7741 ggatcagcgc cccaatcagc gacgccagca tcaaccgtgg caacaaacgc cgcgccccca
     7801 gcattttcgc cagcagcggc gcgaacaaac cgataaagcc gataatcccc accgcattca
     7861 ccagcagcgc actgatgact atcgccagcg acagcgccgc cagacgcgcg agcgacaagg
     7921 ccagcccgag attgcgcgcc acgccatcat caagccccat cagggttaac gggcgaagta
     7981 gcaataacgt caacatcacg ccgcccagca actgcggcca taaacgctca acgccgcccc
     8041 agtcggtttg cgtcagcgtt ccggtgctcc acaggaacat gctttgcagc tggtcgtgat
     8101 ggaagataac cagtagctga ttaattgccc cgcaataaag gctcaccacc agccccgcga
     8161 gaataagcgt aaccggcgaa agccgtttcc cccacgcgac gccaaagacg attaagccaa
     8221 cgacacaagc gcccaccagt gcggcaaact ggctcgccat agcacctggg atcgcccaaa
     8281 gtgtggtgac ggtaattccc agttgcgcgc ctgtagcaac gccaagcgtc gtcggctccg
     8341 ccagcgggtt acgcagcact tgctgaaaca gcacgcccac cagccccagg cccgcgccca
     8401 ccagcagcga aatcgccaga cgcggcaaca agctgtagtg aaaaatcatc tgctcgatga
     8461 catcgatgtc tggcgaccag gcagcctgcg cccactggct acgcggcagc gcctgcgaga
     8521 agttcatcca ggtgagcgcc gtagcgacaa tcactaacag cgccagcaat aacgccggaa
     8581 aaagcgcaat tcgtttactc acgctttacc tccgatggcg ttatccagaa cgcgcacaaa
     8641 gtgcattgcc gagagtgtcg caccataaaa ccagactgca ggtacgcgct gaaagcgtcc
     8701 ggcgcggaca aacggcatgg cctgccacag cggggttgcc attagcgcat ccatgtcttt
     8761 gctgttgtcg tgatcaaaac agagcacatc aacgtcttta tacgctgcca gacgatcgat
     8821 actgacggcg gtactgcccc agaagttggt ttccccttgc caggcatttg ggatgccgta
     8881 ctcatcgaga atttcctgga acaagctgtt tggaccgaag accagcatat ggcgcggatc
     8941 gataagcgtc gtcagcagta acgggcgtgc accacgcttc acaaagcggg gtttcatgct
     9001 gcggataaag tcttcgtatt gcgctaaatg cgtttccgct gcgctttgca ggttaagtaa
     9061 atctgccatt tccgtcagcg atttacgcgc catcgccaac ggctgtttgc cgtcactgaa
     9121 gttaaatccg cgacccggcg caatacgtgc cagcatttct gatgaagggc catatcctgc
     9181 cgaccagacc ataaacgacg gtttcatttc ggtcagcagt tcaaggttag gttctgtacg
     9241 caaaccgacg tcaatcactg agtccggcaa tggtggttcg ctgacccaca ggcgatagtt
     9301 gatggtatcc gccacgccgt aaggcacgat gccgagcgcc agcagtaatt ccaccggcaa
     9361 ccactccagc gccacaatac gattggggtc aatagccgcc gcgtgggcgg tattcatctg
     9421 ccataacaac ggagaaagcg ccatcgccgt taacagtcga cggcgcgaaa taagaggtaa
     9481 gccgctcatc aataaacaaa actcacaggt gcagcacccg ccggatgcgg caaaataccc
     9541 atcgggatgc cataaatcat ttcgagggtt tcgccgcgca taatttccgc aggcgttccc
     9601 tgagcaatca tttcaccact gcgcagggcg accagataat cacagtagcg tgccgccata
     9661 ttgatatcgt gcaacacggc aatgaccgtc aggccacgtt cctgacttaa acggtgcacc
     9721 agcgccagca catcaacctg gtgggcgata tccagcgccg aagtcggttc gtcgagcaac
     9781 agacaacggc tatcctgcgc caccagcatg gcgatccacg cccgctgacg ttcgccgcca
     9841 gagagactat cgaccagccg atgcgccagc ggttttaagc caaccagcga gatagcttcc
     9901 tcgacctttt cgcgatctgc cgcaccaaag cgccccagcg cgccatgcca cgggtaacga
     9961 ccaatcgcca ccagttcacg cacggtcatc ccttctgccg gaggaagctg ctgcggcaaa
    10021 taagccactt tgcgggcaaa cgctttgctg ctccagcttt ccagcggctg ggcatcaaga
    10081 agaatctccc cttctgacgg aggctgatga cgaccaagca ttttgagcag agtggattta
    10141 ccagaaccgt tgtgaccaat cagaccggtc actttcccgg caggaaaggt taacgacagc
    10201 ggatgcaaaa gcgtgcgccc gggcacacga aaggagatat tacgcagtgc aaaagtggta
    10261 tcggaatgat tcgtgtattc ctgcataaca gccaacttgt gaaatgggca cggaaatccg
    10321 tgccccaaaa gagaaattag aaacggaagg ttgcggttgc aacgacctga cgttctgcgc
    10381 cccagaagca gccataagtc tggaagcaac tggcaacata ctcacgatcg agcaaattgt
    10441 tgacgttcac agcaaggctg gatcccgcca taccaaagcg tgccagatca tattttacaa
    10501 cagcatccat tacagctgtg ctacccactt taaaagtgtt tgccggatca ccatagctgg
    10561 aaccaataaa acgaccacct gttcccaatg ttaaaccaga aagcggccct tcattgaagg
    10621 tataatcccc ccagagagat gccatatgct ccggtacttg ttctggagtt ttacctttga
    10681 gattggtgtc tttggtgtat tccgcatcgg tgtaggtata agaagccgtc aagttaatat
    10741 tggcatttaa cgccgctttt gcttcaagtt ccacaccacg agcgcgaatc tcacctgctg
    10801 ggacttgcgc taatgggttt gttgggtctg ctgtcaggtt atttgttttc gtcaattgat
    10861 agactgcgcc cgtaacaacg accggcatat cattcggaac atattttacg ccagcttcat
    10921 actgttcacc tttcgatggc ttataggaaa tgcgcgggtt gctccacaga tcgaaagcac
    10981 tcggttcaaa ggactggcta tagctaaagt aaggtgaaat accattatcg aataagtaat
    11041 ttacaccacc gcgccaggtg aactgatgat cattgcgctc aatataacct tcagtcggcg
    11101 tataagagtt ttcacgaaca gtggtcgctt gcttagacca atcgtagcgt ccccccagag
    11161 tgaataccca tttattccat tccgcctgat cctgaacata aatacctgtt tgtttgcttt
    11221 cattcatctg gtatggctct gcgctaccaa atgcaaagta ttcagggtga tatttgttat
    11281 aaagatcgat ggatggtgcg gatccaaagc tggcattgat atcattacgc atccgcatga
    11341 agtctacccc tgtcagcaaa gtatgctcga cgtcacctgt agcaaactta ctttcaagtt
    11401 gggtatcaac gctaaagttt tgtagacgct cattatccac cactgtcccg cggtttaggg
    11461 tatgaccatc accagcaatc cctgtgccat aaacactttt ctgtgaggtt ttcatttcac
    11521 tgaaacgcag attctgacgc acggtgaagg tgtcattaaa accatgttca aaactatatc
    11581 ccaccatttt ctggttacgg gagtatgtat tattcgatgc accttcattg aagtcagtcg
    11641 gtagacgctt accattaggc aatggctcaa cggtcccctc tttcggcaac caaccgtaat
    11701 aacccgtttc tggttcattc tggaaatagg acaggaaagt aaaattggtt ttgtcgtctg
    11761 gacgccatga gaatgatgga gcaatggtat aacgctgaga ttcagagttt ttctgctgtt
    11821 cgtttgttga gcgagcgagg cccgtcaaac ggtaagagaa ctcaccgtta tcatccagtg
    11881 catcactgaa atcgaatccg gtctgaaaca gattatccgt ccccatttta aactgaattt
    11941 ctttcagggg ttcagtggtc ggacgcttac tcaccataga aataatccca ccaggattac
    12001 tttttccata gagaacagac gtcggcccac gcatcagttc aaccctctcg agcatgtaag
    12061 gatcaatcac agcatcgtta taaaagttgc cctgaagttt tagtccatca aggtaattat
    12121 tttgattcag accaacagat gagaaaccac gaatgataac aaaatcatat gtattagaag
    12181 caccacgact gctaaccgta acgccaggtg tataacctaa tgcttctttt acagactgaa
    12241 attgacgcat ctgcatctct tcatttgtca caaccgaaat agactgcggc gttttttcaa
    12301 ttggtgtatc cgttttagtc gcagtagcag agtgcttcgc cgcaatagtt gcagcaggcc
    12361 cccatgcgct ttcttgcggc gcaggtgcag cggtaacggt gatagtgtct tctttcggtt
    12421 caaccgctgc ctgtgcataa acagacatgc cgctaaccgc tgtggctact acaactgcga
    12481 ttttacgcag tgagtgtttt ggctgagcag ttttggaacg cgccattggt atatctctga
    12541 tgtaaagtga atgataacgt aaacgagaat gattattata aagataagat aatattcgaa
    12601 acgctggcgg gttcgcaagc ggtacgcaat ctgagcgttc ctgaagggtt aataaacaac
    12661 cagatgaaaa gaaagggtta atactttagg tgggatgtta ttttaccgga tggcaattcg
    12721 ccatccggta tttcacgctt agatgttaat tactaccaaa catatccttg atccaaccgg
    12781 ctacaccgtc gctgtctttc tgctcttgct gagcaggttg ctgttgcggc tgttgctgcg
    12841 gctgagaaga ctgatcaaac ggattgcctg acggctgctg ctgcatctcg ctctgctggc
    12901 acagcgattg cggatcgctg gtccagaccg gcaagacacg catgccaccg ctgcaaacaa
    12961 agttgccgtc gtagtccacg cccatatctg caatatcttc tggcggaaca agattcagcg
    13021 gcgttggcgt ctggttagcc agataacgct gataaataga cattgccccg ctggcaccat
    13081 acagtttggt cggctggttg ttatcacggc ctacccaggt gatggtcacc gtgctgccgt
    13141 caatgcccgc aaaccaggta tctacgttat tgttggtagt ccctgttttc cctgccagat
    13201 gcaggttcgg gtatttcgcc ccaagttgac gacccgtacc acgttgtacc acctgctgca
    13261 tggtccatag tgtcagatac gccgcctgcg ccggaacagc gcgttctgcc tgcgggaagc
    13321 tctgatacag caccttgcca tcttccgcga ttaccgaacg cagtgcagaa agcggtgcac
    13381 ggttaccgcc gctggcgatg gtctggaatg cctgcgccac ttcgattggc gttaagttca
    13441 acgcccccag cagcatcgcc ggaaccggat gcagctgatc tttcggcacg cccagtttga
    13501 tccaggtctc cgtaactgca ggcagcccca gcgccatccc cagatttacc gtcggcacgt
    13561 tcatcgaacg ggtcaacgca tccaccagca tcactctgcc gctttcgcta taacgacggt
    13621 catcgttctg cggtgaccag acctggccat tcggctgacg cagcgcgatt ggcgcatcgg
    13681 caatccacgt gttcaggcga tagattttcg gctggcttaa agccgtcaga taagtcgctg
    13741 gttttgcaag ggaaccaatc gaacgacgcg cctgcatcgc acggttgtag cccgcaaact
    13801 gcggctcaga acctcccacc atcgcacgaa cttcaccact aaagcggtcg acgaccacaa
    13861 ttgcagtttc aagatcgctc aacttacgct gtttcttcag tgccggaatg ccttccacgg
    13921 cggctttttc tgccgcgtcc tgggccaccg agtcaaaggt agtgaagatc ttcacgccgg
    13981 agagatcttt taccttatcg cctaatttcg cctgcagctc ctgacgcacc agttgcataa
    14041 aggctggctg tggagagatt accccaccgc gcggctgtac gcccagagga cgcgcgctga
    14101 gcatttcata cagttcacca tcgataatct gctgttgttg tagcaaacgc agcaccagat
    14161 tacgtcgctc cagcgccagt ttcgggttac gccacgggtt gtagatggac gcccctttca
    14221 ccataccgac taacagcgcc tgctggtcga ggctgagctc ttctaccggg cgaccaaagt
    14281 aatacaagct cgcaagcggg aagccgcgga tttcgttgtc gccgctctga ccgagataca
    14341 cctcgttcat atacagctca agaatacggt ctttgctgta acgcgcgtcc atgatcagcg
    14401 ccatgtaagc ttcgttcgct ttacgccagt aagaacgctc gctggagagg aacaggtttt
    14461 tcaccagctg ttgcgtcagc gtactcgctc cctgcaccgt gcgtccggcg gtcaggtttg
    14521 ccagcaccgc acgtccgatg gagtagagac tgatcccatc atgctcgtag aaatgacggt
    14581 cttctgtcgc cagcaaagta tccaccagca agtccgggaa accactgcgc ggcacaaaca
    14641 gacgctgctc accgtttggc gaagagatca tggtgatcag gcgcggatca agacggaaga
    14701 aaccgaactg acggttgttc tccatattga cgatcgtcgc cagatgatcg ccatcaaagg
    14761 tcagacgcgc acgcacctgc ccttctttgc tgtccgggaa atcaaacgga cggcggatca
    14821 tctcaatgct gttggcctgc acggtaaatt cgccaggacg ggtcattttc gacacctgac
    14881 gatactgtgt tgcctccagc agcttcacca tttcgttctt gctgatggtc atatctggtt
    14941 caaggttgac cattcggcca taaactgccg cgggcagttg ccagaccttg ccatcaatac
    15001 ggctacgaat tttttgatcg agataaacgc cgtaaatggc gatcagcacg gcaaaaacga
    15061 tagccagttt taacagcagc catagccagc cgcgtttgcc acgaggctta cgccctttgc
    15121 ctttgccctt acctttgcgc ggcatcggtt cttcatcctc atagtcatca taatcgtcgt
    15181 aatcatcgtc atcttcgtaa cgacgacggc ttaccttttg tttgaccgga cgcgtcggtt
    15241 tccctttgcg tccaattggc tcgcggtcat tcccggccat gctttttctc cgcaatattc
    15301 aggcgcaaaa gcccgatttt ctgttcttct gttacaagac agaagaagat atctctcaaa
    15361 atttgtgctc gatcggtccc ctctcccttg agggagaggg ttagggtgag ggtgaacgtg
    15421 cgcgctgatg ccctcacccc ggccctctcc cacagggaga gggagaaaac caaccttacg
    15481 aatacttttt cgtccgtcgc gtcggtgccg tgtttgccgg gtcgtccggc cagacgtgtt
    15541 tgggataacg ccctttcatc tctttttgca cctcacggta cgctcctttc cagaaggcgc
    15601 tcaaatctcg tgtgatttgt agtggccttt gggcgggtga aagcaactcc agcaccagcg
    15661 gcacgccccc ctgggcgata gtcggattag tggcctcacc aaacatctcc tgcattctca
    15721 ccgccagtgc gggcgggtta tcttcatgat aacgaatggc gatccggctt cccgtcggca
    15781 cagtgtaatg cgcaggcaat tcactatcca gacgttgctg cattccccaa tcaagtaatc
    15841 cgcgtagtgc ctgataaatg tccagtgatt tcaggccgcg tagtgaatgt acgcctgtca
    15901 tatgtggcag cagccacgtt tccagcgctg ccaataaact ttcatcatca accgctggcc
    15961 agtcatattc cggcaaccac tttgcggcgc ataacaaacg caagcgtagc tgttccgctt
    16021 ccgccgtcca gttgagcacg cttaaacctt tatcacggat gccattaagc atcgcctgat
    16081 gcaactcgtc ttctgacggt ttcgccagcg gctgcacttt caccgtcaac tgaccgattt
    16141 gtagccgacg ccaggctttc agcgtacctt gcgcgtcatc ccattccacg gtgtcagatt
    16201 gctgcaccag ctgcggacag cgttgtacta attcatcaat atcgaccggt agcgccagta
    16261 aaatccgcgc atccggcgag gcgctgccct gcaataataa cggtgcgatc aaccattcgt
    16321 ggcggcttag cgcgtcgtcg gcatcgagca tcgctcccat gccgtttgcc agttgatagc
    16381 gtccatcttg cccacggcga tgagcaatgc gatcggcaaa cgccccggca agtagcggcg
    16441 cgataagcga actgtctgcc tcaccgccgc gtacgtttaa gcgttttaac agttgctgac
    16501 tacgttgctg ccaggctggt tgattgcgcg aaaacgccac gcccaggtca ctattgccca
    16561 tcagtggcgg ctcttcgaga atggcggcaa tttttgccgc ggtagcagct tcgtcgtcgt
    16621 tcttcgcgct aaccagcatt gccgctaaac gcggatcgtt acccagcgct gccatttttt
    16681 gcccttgcgc actaagccgt tcaccgtcca gcgcccccag catttgtaac agacgtttcg
    16741 cggccattag attcactact ggcggttgat ccagccagct catctgcgcc ggatcgctgc
    16801 atccccattg cagtaattcc atcagcaaac cggaaagatc gctttgtaag atctccggtt
    16861 cactttgcgc ggcagcgcgt tctgcttgtt ctttggcgat taaatgcagg cagatacccg
    16921 gctccagacg cccggcgcgc ccggcacgct gcgtcatgga tgcctggcta acgcgttgag
    16981 taatcagtcg cgtaagcccc gtgcgcggct caaaacgcgc cacacgctcc tgggcacaat
    17041 ccaccaccag acgaatacct tcaatggtta aactggtttc agcaatattg gtcgccagca
    17101 ccactttgcg catcccttgc ggtgccggga ggatcgcttt tcgctgatcg ttcagcgaca
    17161 acgcgccata cagcgggcag agcaacacat cactgccgat gcgcgaagcc agttgttcct
    17221 gcacacgctg aatttctccg acgccaggta aaaataacag taatgatccg ctttcctgac
    17281 gcagcatttc ggcggtggct accgcaacgg cttcgtcaaa acgctgatgc gcgggcagcg
    17341 gtaaataatg gcgttcaacc ggaaacgagc gcccttctga gatgacgacg ggcgcttctg
    17401 gcagcatttg ctgcaagcgg tcgttatcca gcgtagccga cataatcagc agtttaaggt
    17461 catcacgcag accttgttgc acatcgagta acagcgccaa cgccaaatcc gcctgcaagc
    17521 tgcgctcatg aaattcatca aggatcacca atccaacacc gctcaactcc gggtcacgct
    17581 ggatcatccg cgtcagcacg ccttcggtaa ctacttccag acgggtattt ggcccgacgc
    17641 agttttgcgc acgcatccgg tagccaacgg tatcgcctgg cttttcgtta agcagctccg
    17701 ccagccgttg cgcgacgtta cgtgccgcca gacgacgcgg ctccagcagg ataattttcc
    17761 cgttaatgcc gggatgcgcc agcagttgca gcggcagcca ggttgatttc ccggccccgg
    17821 tcggcgcact taataatacc tgcggcgcac aatcgagagc ggtaagtaat tcaggtaaga
    17881 cggcagcaac gggcaacgac gacacaaaac gctccagagg gttaacattc ttcgcgccac
    17941 attgtagcat cgcggtaatt cataaccgag tgcctcacat gtctgaaccg caacgtctgt
    18001 tctttgctat cgacttacct gcagaaatcc gcgaacagat tatccactgg cgcgccacac
    18061 acttcccacc tgaggcggga cgtccggtcg ccgccgataa tttgcatctg actctggcat
    18121 ttttaggcga agtgagcgcc gagaaagaga aggcgctttc tcttttagcc ggacggattc
    18181 gtcaacctgg tttcacactc acgcttgatg acgccggaca atggctgcgt tcgcgtgtgg
    18241 tgtggttagg gatgcgtcag ccgccacgcg gcttaatcca gctggcgaat atgctccgtt
    18301 cacaggctgc ccgcagcggt tgttttcaaa gcaatcgtcc gtttcatcca catattacct
    18361 tattgcgcga cgccagcgag gcggtgacaa tcccgccgcc aggttttaac tggtcttatg
    18421 cggtgacgga gttcaccctt tacgcctcct cgtttgcccg tggacgcaca cgctacacgc
    18481 cgctaaaacg ctgggcgcta acgcaataac aaggattgtc gcaatggaat tctctccccc
    18541 tctacagcgc gcgacgctaa ttcagcgtta caaacgtttt ttagccgatg tgatcacacc
    18601 cgatggtcgc gaattaacgc tacactgccc gaatacgggt gcgatgaccg gttgtgcaac
    18661 gcgtggcgat accgtctggt attcgacttc agacaacacc aaacggaaat acccacacac
    18721 ctgggaacta actcaaagcc agagcggcgc atttatttgc gtcaatacgc tttgggctaa
    18781 caggttgacg aaagaggcta tccttaatga atcaatttca gaactgtcag gctatagctc
    18841 gctgaaaagc gaagtaaaat acggcgcaga acgcagccgt attgacttta tgttgcaggc
    18901 ggattcgcgt ccagactgct atattgaagt gaaatcggtt acgttagcgg agaacgaaca
    18961 gggatatttt cccgatgcgg tcactgaacg aggtcagaaa caccttcggg agttgatgaa
    19021 cgtagcggct gaaggccagc gtgcggttat ctttttcgcc gtgctgcatt cagccattac
    19081 acggttttca cccgcgcgcc acatcgatga gaaatacgcg caactattgt cagaagctca
    19141 acagaggggg gtagaaattc tggcttacaa agcggaaatt tctgctgaag gcatggctct
    19201 taaaaaatca ctgtcggtta cattgtagta aagtaagtaa ctggttaatt tacattctgg
    19261 tcgcgtgcgc aaatacgctt ttcctcacac agttgtcaag tgttacgttt agataattgc
    19321 tatccggaaa agcatctgct atttatagcg gcctcatttt tcccccgaac atggggatcg
    19381 atagtgcgtg ttaaggagaa gcaacatgca agaagggcaa aaccgtaaaa catcgtccct
    19441 gagtattctc gccatcgctg gggtggaacc atatcaggag aagccgggcg aagagtatat
    19501 gaatgaagcc cagctggcgc acttccgtcg tattctggaa gcatggcgta atcaactcag
    19561 ggatgaagtc gatcgcaccg ttacacatat gcaggatgaa gcagccaact tcccggaccc
    19621 ggtagaccgt gcagcccagg aagaagagtt cagcctcgaa ctgcgtaacc gcgatcgcga
    19681 gcgtaagctg atcaaaaaga tcgagaagac gctgaaaaaa gtggaagacg aagatttcgg
    19741 ctactgcgaa tcctgcggtg ttgaaattgg tattcgccgt ctggaagcgc gcccgacagc
    19801 cgatctgtgc atcgactgca aaacgctggc tgaaattcgc gaaaaacaga tggctggcta
    19861 attacagccg ttccatcacg tttaccacag gcgggaaatg ctcccgcctt attttttgtt
    19921 caaagagatg acagacacac agtatattgg ccgcttcgcc ccctctcctt ccggcgagct
    19981 tcattttggc tctctgatcg ccgcgctcgg cagctatttg caggctcgcg cccggcaagg
    20041 tcgctggctg gtacgcatag aagatatcga cccgcctcgt gaagttcccg gtgccgcaga
    20101 aactatcctg cgccagctgg aacattacgg tctgcactgg gacggcgatg ttctctggca
    20161 atcgcaacgt cacgacgcct atcgtgaagc actcgcctgg ttacatgaac aaggactaag
    20221 ttattactgc acctgtacgc gtgcgcgtat tcaaagcatt ggcggtattt acgacggtca
    20281 ttgccgggtg ttgcatcatg gaccagacaa tgccgcagtg cgtatccgcc agcagcatcc
    20341 ggtcacgcaa tttactgacc agctgcgcgg cattattcac gccgacgaaa aactggcacg
    20401 ggaagatttt atcattcatc gccgtgatgg gttgttcgcc tacaacctgg ctgttgtggt
    20461 tgatgatcat ttccagggcg ttacagaaat agtgcgtggg gctgatctga ttgaaccaac
    20521 agtaaggcaa atctcgctgt accagctttt tggctggaaa gtgccagatt acattcatct
    20581 gccgctggcg cttaatccac aaggcgctaa actttccaag cagaatcatg cgcctgcgtt
    20641 gccgaaaggc gatccacgtc cggtactaat cgcggcactt caatttctgg gtcagcaggc
    20701 agaagcacac tggcaggatt tcagcgtcga acaaatcctt cagtcagcag tcaaaaactg
    20761 gacgctgacc gccgtgcccg agtcggcaat tgtaaattca acattctcaa atgcgtcatg
    20821 ctgagctatg attagccgct atttttttgt cctgaatgat gtttgacact accgaggtgt
    20881 actattttta cccgagtcgc taatttttgc cgcaaggtgc taagccgcga ggaaagcgag
    20941 gctgaacagg cagtcgcccg tccacaggtg acggtgatcc cgcgtgagca gcatgctatt
    21001 tcccgcaaag atatcagtga aaatgccctg aaggtaatgt acaggctcaa caaagcggga
    21061 tacgaagcct ggctggttgg cggcggcgtg cgcgacctgt tacttggcaa aaagccgaaa
    21121 gattttgacg taaccactaa cgccacgcct gagcaggtgc gcaaactgtt ccgtaactgc
    21181 cgcctggtgg gtcgccgttt ccgtctggct catgtaatgt ttggcccgga gattatcgaa
    21241 gttgcgacct tccgtggaca ccacgaaggt aacgtcagcg accgcacgac ctcccaacgc
    21301 gggcaaaacg gcatgttgct gcgcgacaac attttcggct ccatcgaaga agacgcccag
    21361 cgccgcgatt tcactatcaa cagcctctat tacagcgtag cggattttac cgtccgtgat
    21421 tacgttggcg gtatgaagga tctgaaggac ggcgttatcc gtctgattgg taacccggaa
    21481 acgcgctacc gtgaagatcc ggtacggatg ctgcgcgcgg tacgttttgc cgccaaattg
    21541 ggtatgcgca tcagcccgga aaccgcagaa ccgatccctc gcctcgctac cctgctgaac
    21601 gatatcccac cggcacgcct gtttgaagaa tcgcttaaac tgctacaagc gggctacggt
    21661 tacgaaacct ataagctgtt gtgtgaatat catctgttcc agccgctgtt cccgaccatt
    21721 acccgctact tcacggaaaa tggcgacagc ccgatggagc ggatcattga acaggtgctg
    21781 aagaataccg atacgcgtat ccataacgat atgcgcgtga acccggcgtt cttgtttgcc
    21841 gccatgttct ggtacccact gctggagacg gcacagaaga tcgcccagga aagcggcctg
    21901 acctatcacg acgctttcgc gctggcgatg aacgacgtgc tggacgaagc ctgccgttca
    21961 ctggcaatcc cgaaacgcct gacgacgtta acccgcgata tctggcagtt gcagttgcgt
    22021 atgtcccgac gtcagggtaa acgcgcatgg aaactgctgg agcatcctaa gttccgtgcg
    22081 gcttatgacc tgttggcctt gcgagctgaa gttgaacgta acgctgaact gcagcgtctg
    22141 gtgaaatggt ggggtgagtt ccaggtttcc gcgccaccag atcaaaaagg gatgcttaac
    22201 gagttggatg aagagccgtc accgcgtcgc cgtactcgtc gtccacgcaa acgcgcaccg
    22261 cgtcgtgagg gtaccgcatg acagtggcgt atattgccat aggcagcaat ctggcctctc
    22321 cgctggagca ggtcaatgct gccctgaaag cattaggcga tatccctgaa agccacattc
    22381 ttgccgtttc ttcgttttac cgcactccac cgctggggcc gcaagatcaa cccgattacc
    22441 tcaacgccgc cgtggcgctt gaaacttcgc tcgccccgga agagttactc aaccatacac
    22501 agcgcataga actgcaacag ggtcgtgttc gtaaagctga acgctgggga ccacgcacgc
    22561 tggatctcga tatcatgctg tttggtaatg aagtgataaa taccgaacgc ctgaccgttc
    22621 cgcactacga tatgaagaat cgtggattta tgctgtggcc gctgtttgag attgcaccgg
    22681 agctggcgtt tcctgatggg gagacgttgc gtgaagtctt acatacaaga gcatttgacg
    22741 aattaagcaa atggtaattt atttatatta cgaatataaa tattttctga aaatcataca
    22801 tcctctcttt ccttaataaa aattttagtt taaacatttt tgcagaaaca taatataaat
    22861 tcccgcagat aaatatcaat cccatcgcca taccctaacc aattgcactt gtgaatttat
    22921 ttttgcttaa ttttgaatta agaaaattta atttgttcaa aagcccttaa agtgcacaat
    22981 aggcgtccta atgaacaatg gttgtaaatt ttggtaacaa atatcattgc tcattaatat
    23041 ctagcatctg cttataacat ttgtgtcatg aatagtcatt cttcaaacca gtagtctgac
    23101 taatggaatt ttattcaagg atttttatgt ctaaaaaatt aggttttgcc ctgtctggtc
    23161 ttatgctggc aatggttgca ggtactgctt ctgctgatat ggatggtggt cagttaaata
    23221 tcagtggtct ggttgttgat aacacctgtg aaacccgcgt tgacggcggt aataaagatg
    23281 gtctgatcct gctgcaaact gcaactgttg gtgaaatcga tgctggcgtg ctgaatgaca
    23341 ccgttggcgc taaagccaaa cctttcagca tcaccgttga ctgcagcaaa gcaaatccga
    23401 atccaggtag tacagctaaa atgaccttcg gttctgtttt ctttggtaat agcaaaggaa
    23461 cgctgaacaa cgacatgtct attaacaacc cgtctgacgg cgtgaatatc gctctgcaca
    23521 atattgatgg ttccactatc aaacaggttc aaattaacaa ccctggcgat gtgtatacta
    23581 aagccctgga tgcaacgaca aaatctgctg tttatgattt taaagcgtct tacgttcgtg
    23641 ccgtcgcaga ccaaacagca actgctggtt atgtaaaaac taacactgca tacaccatta
    23701 cttatcagta agtattcacc aattaaattg attgaatgaa tatacaggga atagtgttaa
    23761 taatttctat ttttatatta ttccctgttt taattaactc tgtcagggat ggtttatgtt
    23821 ttttaacacc aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc
    23881 gtctattgcg gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag
    23941 tgtcaacgta cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga
    24001 tactggcgat aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc
    24061 agtatcgcgt attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac
    24121 ctcactgcct aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa
    24181 accagatgca gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat
    24241 aaaacttttc tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct
    24301 gaagtggttc tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc
    24361 ttactacgtc tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga
    24421 tgtgaaaatg attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa
    24481 agcaagttct gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg
    24541 taatgccagt ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa
    24601 aaccaactaa aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt
    24661 attgcaatag tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc
    24721 agaatgcctc aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta
    24781 tgtacgacgt cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta
    24841 ttgaaattga aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt
    24901 tcatataaat tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac
    24961 actcggcaat tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt
    25021 taacgagcaa cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa
    25081 ctatgttgat ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct
    25141 caacggatat catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa
    25201 cggtgggatg aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac
    25261 cgattctggc agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct
    25321 gcgttctcaa ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag
    25381 tatccgcggc attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt
    25441 tgcgcctatc attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg
    25501 ctataagatt tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc
    25561 gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac
    25621 attctcgcaa cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga
    25681 tattagcggc ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc
    25741 cagctactac tacggtctga ataactatct aacg
//
LOCUS       DAJTVK010000051        25724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000051 DAJTVK010000000
VERSION     DAJTVK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25724
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.051"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            124..447
                     /gene="soxS"
                     /locus_tag="OO372_002153"
     CDS             124..447
                     /gene="soxS"
                     /locus_tag="OO372_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6341645.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="OO372_002154"
     CDS             complement(450..2036)
                     /gene="pdeC"
                     /locus_tag="OO372_002154"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR6341646.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2466..2746
                     /locus_tag="OO372_002155"
                     /pseudo
     CDS             2466..2746
                     /locus_tag="OO372_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="OO372_002156"
     CDS             complement(2845..3381)
                     /gene="ssb1"
                     /locus_tag="OO372_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6341647.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3635..6457
                     /gene="uvrA"
                     /locus_tag="OO372_002157"
     CDS             3635..6457
                     /gene="uvrA"
                     /locus_tag="OO372_002157"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6341648.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6492..6848)
                     /locus_tag="OO372_002158"
     CDS             complement(6492..6848)
                     /locus_tag="OO372_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6341649.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6852..7268)
                     /locus_tag="OO372_002159"
     CDS             complement(6852..7268)
                     /locus_tag="OO372_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6341650.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="OO372_002160"
     CDS             complement(7379..8092)
                     /gene="aphA"
                     /locus_tag="OO372_002160"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6341651.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8494..8716
                     /locus_tag="OO372_002161"
                     /pseudo
     CDS             8494..8716
                     /locus_tag="OO372_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="OO372_002162"
     CDS             complement(9220..10413)
                     /gene="tyrB"
                     /locus_tag="OO372_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6341652.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10666..11745)
                     /gene="alr"
                     /locus_tag="OO372_002163"
     CDS             complement(10666..11745)
                     /gene="alr"
                     /locus_tag="OO372_002163"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6341653.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="OO372_002164"
     CDS             complement(11798..13213)
                     /gene="dnaB"
                     /locus_tag="OO372_002164"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6341654.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13296..14279
                     /locus_tag="OO372_002165"
     CDS             13296..14279
                     /locus_tag="OO372_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6341655.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="OO372_002166"
     CDS             complement(14445..14687)
                     /gene="pspG"
                     /locus_tag="OO372_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6341656.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="OO372_002167"
     CDS             complement(14821..15858)
                     /gene="dusA"
                     /locus_tag="OO372_002167"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6341657.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16221..17206)
                     /locus_tag="OO372_002168"
                     /pseudo
     CDS             complement(16221..17206)
                     /locus_tag="OO372_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            17524..18039
                     /gene="zur"
                     /locus_tag="OO372_002169"
     CDS             17524..18039
                     /gene="zur"
                     /locus_tag="OO372_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6341658.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(18081..18290)
                     /locus_tag="OO372_002170"
     CDS             complement(18081..18290)
                     /locus_tag="OO372_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6341659.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="OO372_002171"
     CDS             complement(18406..19731)
                     /gene="dinF"
                     /locus_tag="OO372_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6341660.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="OO372_002172"
     CDS             complement(19804..20412)
                     /gene="lexA"
                     /locus_tag="OO372_002172"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6341661.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="OO372_002173"
     CDS             complement(20522..20890)
                     /gene="dgkA"
                     /locus_tag="OO372_002173"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6341662.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            21061..23484
                     /gene="plsB"
                     /locus_tag="OO372_002174"
     CDS             21061..23484
                     /gene="plsB"
                     /locus_tag="OO372_002174"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6341663.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="OO372_002175"
     CDS             complement(23639..24511)
                     /gene="ubiA"
                     /locus_tag="OO372_002175"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6341664.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(24523..25020)
                     /gene="ubiC"
                     /locus_tag="OO372_002176"
     CDS             complement(24523..25020)
                     /gene="ubiC"
                     /locus_tag="OO372_002176"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6341665.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(25243..25437)
                     /locus_tag="OO372_002177"
                     /pseudo
     CDS             complement(25243..25437)
                     /locus_tag="OO372_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25564..>25724
                     /locus_tag="OO372_002178"
     CDS             25564..>25724
                     /locus_tag="OO372_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6341666.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSS"
BASE COUNT         6663 a         6607 c         6157 g         6297 t
ORIGIN      
        1 gttagcagcg ctttaatgcg gggtaatttc ttttccataa atcgctttac ctcaagttaa
       61 cttgaggaat tatactcccc aacagatgaa ttaacgaact gaacactgaa aagaggcaga
      121 tttatgtccc atcagaaaat tattcaggat cttatcgcat ggattgacga gcatattgac
      181 cagccgctta acattgatgt agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa
      241 cgaatgttcc gcacggtgac gcatcagacg cttggcgatt acattcgcca acgtcgcctg
      301 ttactggccg ccgttgagtt gcgcaccacc gagcgtccga tttttgatat cgcaatggac
      361 ctgggttatg tctcgcagca gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact
      421 cccagcgatt atcgccaccg cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt
      481 aattgctcca tccattgcat aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc
      541 tggcaatagc gcacgccgcg tttgcgcagc cagttaacct gctcctcagt ttcgacgcct
      601 tcagcgatcg tttttaaccc caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa
      661 tgactggttt tgtgggtggt cagcgtttca acaaacgatt tatcgatttt caaaatatcg
      721 acattcaatg atttaaggtt atgcaagtta gagtagccaa taccaaaatc atcaattgcc
      781 acttcgtaac cagcctggcg gaaagccaga ataatcggcg tcattttttc gacatcaaga
      841 aatgcatgct cagtcacttc aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt
      901 ttctgattga ttcgcgctat caaccgtgac gtatgaaaat cggaggccga caggttaata
      961 gaaacatagc gatcggcatg tgttgccaag tacgcgccca gatcgcggaa gacattatca
     1021 ataacataat cagttatctg ttctatcatc ccctcctttt ctgccagcgg aataaactct
     1081 gccggattca ttatttgccc ctgctcacca agccaacgta acaacgcttc agcgccgata
     1141 catttttctg ttttgatatc gattattggc tggtaataaa gacaaagttg atgtttttcg
     1201 agggcgcgtt gcaatttacg tttgggagat aaatagtttt gtcgaatacg tagccagagt
     1261 aatagcagaa caagactccc caaaataccg gcgggcaacg caaaaataag atgattataa
     1321 aaatgggtta taagacgttg atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc
     1381 gaataaactg tcgcatataa atagccattt ctttgtacgg ttaaatcctt caaatgaatc
     1441 agcggagaaa acgttgctgc cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc
     1501 gtatcataca caccccattg caatgtcggg tcatcagaca tcacttcact ccagaagaga
     1561 gggttgataa ccgccacata atttccccgc tgcatatagg tcattttata gccagagaaa
     1621 aaaggcgtat cgcggtaata atagatagaa acgttaggtt cacgcttata atcggccggt
     1681 gcaatcgtat agccgtttac aggcgctatc agcgatgagc ataaaaaatg gttatcacgg
     1741 gcatagatca attcattaat ataaagatag ccacgaataa tattcaacat tcgcttttga
     1801 tgggctggag tgcaaacttg cccctgatag cgttcagctg catcgctcac taaatctgcc
     1861 tggcgaatga ccagctcgga tttatccagt gccagttgag caaaggttcg gagctgatta
     1921 ttcacttctg attttgccca aaggaaggca atccatagcg aaagaatgat gggaaagaga
     1981 actaaaaaga taatgcccgg caacgccagt aattggtgtc gtgcacgatg actcattgct
     2041 atcaatttcc tgcctgttta tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt
     2101 ctttagataa atcttacggt ataaatagca cttttgcctg atttgaaatt gatacctttc
     2161 taatcgtcac ctggatgaaa ataattcatt gatattcaaa attaatttcc tacaggaatg
     2221 cagaggcggc gggaagatat gcccttaatg ccactgaaaa accacataat ctgtacagca
     2281 ataaaagtca cggcctaata tggctaacaa acaatcaatt gtgatatagc tcacaaaatt
     2341 aatgaaacaa acagagtgtt tcatttttgt gttctgcaag ccccgcgata gccagagtgt
     2401 accacgcctc ccgtgaacaa cgccgcgctg tccagggcat cggctctttt cagaggaatt
     2461 gtttgatggc taccctcacc actggcgtgg ttcttcttcg ctggcagctt cttagtgccg
     2521 taatgatgtt tctggccagc acatcaacat ccgttttcgt cggtctgatt atgtcgggct
     2581 tgcggtgatc agcagcggtc tgggcgttgt ttccgcctgt tggttcgcaa tggggttgct
     2641 tggcatcaca atggcggaca tcaccgctat ctggcacaac atcgagtcgg tgatgataga
     2701 agagatgaat cagacaccgc cacaatggcc aatgattttg acctgatacg tagaagcctc
     2761 cataacggag gcttcttttt tatggctggc agatgcttta atcatccacc ttaaaacaat
     2821 ataacctatt gttttaatga caaatcagaa cggaatgtca tcatcaaagt ccatcggcgg
     2881 ctcgttagac ggcgctgccg gagcggactg ctgcgggcga gactgcgcgc cgccgctgaa
     2941 ctgattgcca ccctgcggct gctgaggctg cccccaaccg ccctgcggct gaccaccacc
     3001 gatattacca cctgccggag cgccaccacc ctgacgacca cccagcatct gcatggtgcc
     3061 gccaacgttc accacgactt ctgtggtgta gcgatcctga ccggattgat cggtccattt
     3121 acgggtacgc agctgacctt cgatataaac ctgagaacct ttacgcagat attcgctggc
     3181 cacttctgcc agtttgccga acagcacaac gcggtgccat tcagtctgtt ctttcatctc
     3241 gccggtcgct ttatcacgcc aggattcgga agtagccagc gtaatgttgg caactgcgcc
     3301 accatttggc atgtagcgta cttccgggtc ctgacccaga ttaccaacga gaataacctt
     3361 gtttacgcct ctgctggcca tgttcgtgtc tcctgaaaaa aatcgttctg aataagtgta
     3421 aacgcgcgat tgtaccatta ccaatagcgc ttttactatg ttgtgacctc ggttccggga
     3481 aacaaacctg gccagacatt gttacacaac actccggata atgcattcca atactgtata
     3541 ttcattcagg tcaatttgtg tcataattaa ccgtttgtga tcgccggtag caccatgcca
     3601 ccgggcaaaa aagcgtttaa tccgggaaag gtgaatggat aagatcgaag ttcggggcgc
     3661 ccgcacccat aatctcaaaa acatcaacct cgttatcccc cgcgacaagc tcattgtcgt
     3721 gaccgggctt tcgggttctg gcaaatcctc gctcgctttc gacaccttat atgccgaagg
     3781 gcagcgccgt tacgttgaat ccctttccgc ctacgcgcgg cagtttctgt cactgatgga
     3841 aaagccggac gtcgatcata ttgaggggct ttctcctgcc atctcaattg agcagaaatc
     3901 gacgtctcat aacccgcgtt ctacggtggg gacaatcacc gaaatccacg actatttgcg
     3961 tttgttgttc gcccgcgtcg gcgaaccgcg ctgtccggac cacgacgtcc cgctggcggc
     4021 gcaaaccgtc agccagatgg tggataacgt gctgtcgcag ccggaaggca agcgtctgat
     4081 gctgctcgcg ccaatcatta aagagcgcaa aggcgaacac accaaaacgc tggagaacct
     4141 ggcaagccag ggttacatcc gtgctcgtat tgatggcgaa gtctgcgatc tttccgatcc
     4201 gccgaaactg gaactgcaaa agaaacacac tattgaagtg gtggttgatc gcttcaaggt
     4261 gcgtgacgat cttacccaac gtcttgcgga gtcgtttgaa accgcgctgg agctttccgg
     4321 tggtacagcg gtagtggcgg atatggacga cccgaaagcg gaagagctgc tgttctccgc
     4381 caacttcgcc tgcccaattt gcggctacag tatgcgtgaa ctggagccgc gactgttttc
     4441 gtttaacaac ccggcaggtg cctgcccgac ctgtgacggc cttggcgtac agcaatattt
     4501 cgatcctgac cgcgtgatcc aaaaccccga gctgtcactg gctggcggtg cgatccgtgg
     4561 ctgggatcgc cgcaacttct attatttcca gatgctgaaa tcgctggcag atcactataa
     4621 gttcgacgtc gaagcgccgt ggggcagcct gagcgcgaac gtgcataaag tggtgttgta
     4681 cggttctggc aaagaaaaca ttgaattcaa atacatgaac gatcgtggcg atacctccat
     4741 tcgtcgtcat ccgttcgaag gcgtgctgca caatatggag cgccgttata aagagacgga
     4801 atccagtgcg gtacgtgaag aattagccaa gtttatcagc aatcgcccat gcgccagctg
     4861 cgaaggaacg cgtctgcgtc gggaagcgcg ccacgtgtat gtcgagaata cgccgctgcc
     4921 tgctatctcc gacatgagca tcggtcatgc gatggaattc ttcaacaatc tcaaactcgc
     4981 aggtcagcgg gcgaagattg cagaaaaaat ccttaaagag atcggcgatc gtctgaaatt
     5041 cctcgttaac gtcggcctga attacctgac actttcccgc tcggcagaaa cactttccgg
     5101 cggtgaagcc cagcgtatcc gtctggcgag ccagattggt gcgggcctgg ttggcgttat
     5161 gtatgtactg gacgagccgt ctatcggcct gcaccagcgc gataacgagc gcctgttggg
     5221 tacgcttatc catctgcgcg atctcggtaa taccgtgatt gtggtggagc atgacgaaga
     5281 cgcaattcgc gccgctgacc atgtgatcga tatcggtcct ggtgcgggtg ttcacggcgg
     5341 tgaagtggtc gcagaaggtc cgctggaagc gattatggcg gtgcctgaat cgttgaccgg
     5401 gcagtacatg agcggtaaac gcaagattga agtgccgaag aaacgcgttc cggcgaatcc
     5461 agaaaaagtg ctgaagctga caggtgcacg cggcaacaac ctgaaggacg tgacgctcac
     5521 gctgccagtc ggtctgttta cctgcatcac aggggtttca ggttccggta aatcgacgct
     5581 gattaacgac acactgttcc cgattgccca acgccagttg aatggtgcga ccatcgccga
     5641 accggcaccg tatcgcgata ttcaggggct ggagcatttc gataaagtga tcgatatcga
     5701 ccaaagccca attggtcgta ctccacgttc taacccggcg acctataccg gcgtgtttac
     5761 acctgtgcgc gaactgtttg cgggcgtacc ggaatcccgt gcgcgtggtt atacgccagg
     5821 acgtttcagc tttaacgtcc gtggcgggcg ctgcgaagcc tgtcagggcg acggtgtgat
     5881 caaagtggag atgcacttcc tgccggatat ctacgtgccg tgcgatcagt gcaaaggtaa
     5941 acgctataac cgtgaaacgc tggaaattaa gtacaaaggc aaaaccatcc acgaagtgct
     6001 ggatatgacc atcgaagagg cgcgtgagtt ctttgatgcg gtgccagctc tggcgcgtaa
     6061 gctgcaaacg ttgatggacg ttggtctgac gtacattcgc ctggggcagt ccgcaaccac
     6121 actttctggt ggtgaagccc agcgcgtgaa gctggcgcgt gagctgtcaa aacgcggcac
     6181 cgggcagacg ctgtatattc tcgacgagcc gaccaccggt ctgcacttcg ccgatattca
     6241 gcaactcctc gacgtgctgc ataaactgcg cgatcagggc aataccattg tggtgattga
     6301 gcacaatctc gacgtgatca aaaccgctga ctggattgtc gacctgggac cagaaggcgg
     6361 cagtggcggc ggcgaaatcc tcgtctccgg tacgccagaa accgtcgcgg agtgcgaagc
     6421 ttcgcatacg gcacgcttcc tcaagccgat gctgtaatcg ttaaggccgc tttctgagcg
     6481 gccttttcct ttcagagttg taccagcaat ttacgctttt cttccggtag taaattcact
     6541 gcctgctgat aagacgcatc caccagataa tagatttgcg aatccggcag cgaaccgtcg
     6601 agatacacgg tgctccagtg cgctttattc agatggcggc taggacgcac atcgctgtgc
     6661 tgctgacgta gcagctccgc cagctccggg ctggttttca gcgaaacagc tgggcgattt
     6721 tctacttctt tcaccatcgc aaacagtaca tcttccactt tgatctgcgt cgctttccag
     6781 tcgttatgca cgctctgttc tgcgcctggt tttgccatgc aatattgtag caactccgaa
     6841 atggtcattt tttactcccc ttgtagtgtc gcgatgatgc gacgcgatcc gccgtggatg
     6901 cgatgttccc ccagccagat gccttgccag gtgccggtct gaatacgccc tttatgtaac
     6961 ggcaatacaa gcgatgttcc cagcattgag gatttgatat gagaaggcat atcgtctgct
     7021 ccctcatagt catgctcata atttccgttg tcgggaacgg tgcggaggaa aaaacgctcc
     7081 atgtcgtggc gtacggtggg atcgcagttc tcattaagtg tcagagaggc ggaggtatgt
     7141 tgcagcaaca gatgcagtaa gccgatgtta acgcgcggca tatcagccag ctgattcaga
     7201 atttcatccg ttaccagatg aaacccacga gatttggcgc taagcgtgag cgtcttttga
     7261 taccacatgt gctgctcctt gataaaactc tcttaatcag tttgcagcaa gacagcgaaa
     7321 ggataaaggt gtgattaaaa aaacagcatt cgggagagca tcacgctctc ccgctctgtc
     7381 agtattctga attgacgatc acctcttcac caaacgcacc cgcttgtggc aagggtttgt
     7441 aggtagagtt ggaggcgcgc agaatgcgga taccacgagc gccgacatcg cgtgcggcgg
     7501 taatatcgtt atcggaatcg ccataaaaaa ttcggatatt tttatcctgc agccattgcg
     7561 attttgtatt ttgccctggt ttatcgcccg caaagattac cggattcatg ttggtggcag
     7621 gaatatgaaa attatccgcc agcgtttttg aaaccgtttc tgttttcgtc gggctacgac
     7681 cagtcacaaa gaagatcgcg tcaccgcggc gtacatgcat atcaatcagc tggcgagcga
     7741 cctcttttgg aatgctgaat tcatcccagc cattattcat tttttcccag aacacaggat
     7801 ttttcagata atcttcgctt tctggcgaga aggttttttt gccgcgccag aaccccggac
     7861 tggaaaaaag taccgtgtca tcgatatcaa accccaccgc cattggcgga cgccctgcga
     7921 ggctattttc aatttgtgcg accgaaaccc aatgaattgg tgcctgttca gcaagcctgg
     7981 caacgttagt cccagggtta agcggtgaag gagatgaggc cagggcaaca gcggaactgt
     8041 ttagagcgaa caataagcaa acggcactga ttgcctgtgt gatcttgcgc atatttttcc
     8101 ctaaatagtc agtatgttga aactttttat tgtgagattt gttgcaaaaa ccatctgacc
     8161 ataacgccag ctgccgggga aaggaaggat tatctgcggt ttttgtgatg aaaagcagga
     8221 tgattatatt taacccagta aatacgttcc tgtctttttt tgtataaaaa atgtttatta
     8281 ctcgatgtaa ggatacatta attttacgtt tattatgatg aatcctctgc aaatgagcga
     8341 aatttgaaag aacatttatc tgctgtacga tttaatgaac ttaatgatac agcattacaa
     8401 aaacaatcga agtttataaa gatgattcct gattgaccag aaccctatcc tacaatggtg
     8461 actaatttta ttactcccga tggagatgat gatatgaata tatcatatgt gaatagcaac
     8521 aaaacgacat ctttacctgt tgagctagat gcactaaata ataaagacat ctcttatgcc
     8581 aaagattttt cttatgctaa agatttcttt ttatacatag aaacacagtt aaaaattgca
     8641 aaagattttt gtagacctgg agaagaagta tcaagttcta ttgcaagtaa agtttttcac
     8701 gcatttatcg atttagttaa caaaatcagg ggtaagaaag actttatgta tatttgcacg
     8761 ctttgctgtt ttgccgagga ggttaaaggt gattattctc attacaggac ctttttattt
     8821 gatattggta atcaatataa ggttaaactt acacaaagcg gaaaaaaaga gttctcttta
     8881 acattagaat ttaacgatac tataattgaa tctcagaaag tcacaggcaa taaggcaaag
     8941 tatattcttg aggatataga aatattctat cgtaacaaac ccgatactta ttattaaatt
     9001 taataatggt tagatgtgca gaaattatta ctccacacga aaaaataggg aaaggaattc
     9061 cagataattg ggataaactt aacaataaaa gaactgacaa cgttttatta tctctcacgt
     9121 agaacaacgg catcaaaaaa atcatctaca aagaaatagt gaaagcgacg acagtttaat
     9181 ttcactgcag gctgggtaac tccagcctgc tttcctgcat tacatcaccg cagcaaacgc
     9241 ctttgccaca cgttgtacat ttgccgtatt taacccggcg acacacatgc gaccgctggc
     9301 gatgagatag acaccaaatt cttcacgtag tcggtcaacc tgagcggcac ttaaaccggt
     9361 ataactgaac atgccgcgct gattaagcag ataatcgaaa ttgcgttctg gcatctctgt
     9421 gcttaatacc ttcaccaatt cctgacgcat tgccagaatg cgagtacgca tctcttctac
     9481 ttccgccagc cagctggctt tcaatgcctc gtcattcagc actgcagcca ccacctgcgc
     9541 accaaaattc ggcgggctgg agtagttgcg gcgaactgtt gctttcaatt gccccagtac
     9601 gcggccagcg gcttcggcat cttcacacag aacagaaagt ccgccgacgc gctcgccgta
     9661 aagggagaaa attttcgaga acgaattgct caccagagcg ggtaatccag cgctggcaat
     9721 ggcgcgaata gcgtaggcat cctcttccat accggcacta aatccttgat aggcaatatc
     9781 gaggaatggg ataagctcgc gggctttgag aatttcaatc accgcatccc actgatcatt
     9841 agtgagatcg gcacccgttg ggttgtggca acatggatgc agcaacacaa tactgcgggc
     9901 aggtaatgtt ttcagcgtcg ccaacaggtc attaaagcgc acgctgttag tcgcttcgtc
     9961 ataccagggg taagtactca cttcgaatcc agccccggcg aatattgcta cgtggttttc
    10021 ccaggtagga tcgctgaccc agacgcctga ttccgggaag tagcgtttca ggaaatccgc
    10081 gcccactttc aatgcccctg agccgccaag ggtttgaatg gttgctacgc gctgttgttg
    10141 cagtaccgga tggtccgcac caaacagcag cggcgcaatg gcatggcgat agctgttaag
    10201 cccttccatc ggtaaataaa gcgaagcgcc atggggctgc gcattcaggc gcgcttccgc
    10261 ctccgccacg gctttcagtt gtggaataat tccgtcttcg ttgtagtaca gaccgatact
    10321 taaattcact ttgtcgctgc gagggtcttc tttaaaacgc tccataagcg taagaatcgg
    10381 atcgccagcg taggcgtcaa ctttttgaaa cacgcgatgg ttctccaggt ttacgggcag
    10441 gtggttaaaa cacaataaac cggaagaagg cgaagatcga gtggatgttc aggagcgaac
    10501 ggcaattagc aacagagtga gactcatgac aaacgaacat ccgccagagg cacgaccttt
    10561 ataagagcat gaaagaacat caacttattg aatttttagg attttattgg ccggataagg
    10621 cattcacgcc gcatccggca cagacaatca aatattacag aacgattaat ccacgtattt
    10681 catcgcgacc cttgaagtca ggcgcgtaat aagttcgtaa gcgctcactt ttgtcatttc
    10741 agcgatacgt tctacgggca aaccttcgcc ccataaaatg accggatccc cggctttgtc
    10801 ctgcgcctgt ggacctaagt ctacgcagat catatccatc gccacgcgtc cgacaatcgg
    10861 tacttcgcga ccgttcacca gcactggcgt accggatgga gcggcgcgcg gataaccatc
    10921 gccatagccc atcgcgacta cgccaagacg ggtatcacgt tcgcttaccc aggttccacc
    10981 ataaccaaca ggctctccgg ctttatgctc acgcacggca atcaggctgg aggttagtga
    11041 catcactggc tgacagccaa aatcggcacc gatggagcga tcttccagcg gcgagacgcc
    11101 ataaagaatg atgcccgggc gcacccagtc aaaatgcgat tgtggccaca gcagaatgcc
    11161 acccgacgcg gcaatggaac gttgaccagg tttgccttcg caaaaggtat taaagatagc
    11221 gagttgtttc tcggttgcgc cacattttgg ttcatccgcg cgcgcaaaat ggctgacgat
    11281 attcaccggc tgacgaacgt ttttgcactg tgtcaggcga tgataaaacg cctcagcctg
    11341 ttccggcctt acgcccagac ggtgcatacc ggtatcgagt ttcatccaga cggtaaccgg
    11401 ctcgtccagg ctagcctctt ccagcgcagc cagctgttct tcgttatgca cggcggtatg
    11461 aaaatgttgc gcagaaatcg tcggcagatc tctggcatca aaaaagcctt cgagtaacag
    11521 tacaggtttg gtgattcccc ccgcacgcag tcgcagagct tcttcgagac gggctacgcc
    11581 aaaggcgtca gcatcgggga gcgttcgcgc ggtctcaaga agaccgtgac cataagcgtt
    11641 cgctttcacc accgcaacca ttttactggc aggggccagt tcacgaagac gttgcaggtt
    11701 gtgtcgcaga gcgcggcggt taatcacaac agttgccgct tgcatttgtg ttccttgata
    11761 agtgtttgct ttaattacct aattcataaa ataattatta ttcgtcgtcg tactgcggcc
    11821 ccgcatagtt gtcgaagcgc gaccattgac cgttaaaggt caggcgtacc gtcccgattg
    11881 ggccgttacg ttgtttaccg ataataattt ccgcgatgcc ttttaaatca ctgttttcgt
    11941 gatacacctc atcacgatag ataaacataa tcaagtccgc atcctgctcg atagagccag
    12001 attcacgcag gtcggagttg accgggcgtt tgtcggcacg ttgttccaga gaacggttca
    12061 actgggacag cgccaccacc ggcacgttca gttctttcgc cagtgctttc agcgagcgag
    12121 agatttctgc aatttccagc gtacggttat cggaaagcgc cggtacgcgc atcagttgca
    12181 ggtagtcgat catgataagc ccgatgccgc cgtgttcacg ggcaatacgg cgtgcgcggg
    12241 aacgcacttc cgttggcgtc aggccggagg agtcatcgat atagatattg cgtttttcga
    12301 gcaaaatacc catggtgccg gaaatgcgcg cccagtcttc atcatcgagc tgcccggtac
    12361 ggattttagt ctggtcaacg cgcgacagcg acgccagaga acgcatcata atctgttctg
    12421 aaggcatctc cagcgagaag ataagtactg gtttatcctg caacatcgcc gcgttttcga
    12481 cgaggttcat cgcaaatgtt gttttaccca tcgacggacg cgcggcgacg atgatcaaat
    12541 ccgacggctg caagccagcg gtttttttgt tgagatcgtc ataaccggtg tttactccgg
    12601 taacgccatc gtgtggctgc tgaaacaact gctcaatacg cgccacggtt gcgtcgagca
    12661 catcggcgat gttcttcggc ccttcgtctt tgtttgcacg actttcggca attttaaaga
    12721 cgcgggattc agcaaggtcc agcagatctt cgctggtacg cccctgcgga tcaaaaccgg
    12781 cttcagcaat ctcattcgca accgagatca tctcacggac aacggcacgt tcacgcacga
    12841 tgtcagcata agcactgatg ttcgccgcac ttggcgtatt ttttgacagc tctgccagat
    12901 aagcaaaacc accgacgcta tcgagttgcc cctggcgttc cagcgattcc gcaagggtaa
    12961 tcagatcgat aggactaccg ctttcctgca aacgcgccat ttcagtaaag atatggcggt
    13021 gtgggcgggt gtaaaaatcg tctgccacca cacgctcggc tacatcgtcc cagcgttcgt
    13081 tatccagcat taaaccgccc aacaccgact gctccgcttc gatcgagtgc ggaggcactt
    13141 tcagcccggc aacttgtgga tcgcgttcgc ggggttcagc ctgctgtttg ttgaagggtt
    13201 tatttcctgc catagtgaat ggagttaccg agataaagaa tgggtcgaaa ctttaccata
    13261 tgaagcagac cctgacgata cgttctggag gacacatggc aacacgaatt gaatttcaca
    13321 agcacggtgg cccggaagta cttcaagccg tagagttcac tcctgccgat ccggcagaga
    13381 atgaaatcca ggtcgaaaat aaagccatcg gcatcaattt tatcgacacg tatatccgca
    13441 gcggccttta cccgccgcca tcactaccca gcggattagg taccgaagcg gcaggcatcg
    13501 tgagtaaagt cggcagtggt gtaaagcata ttaaggcagg cgatcgtgta gtctatgcgc
    13561 agtcggcgtt aggcgcttac agctctgtgc ataacattaa tgcggataaa gcggcgattc
    13621 tgcctgcggc aatttctttt gagcaagctg cggcatcctt cctgaaaggc ttaacggttt
    13681 attatctgct gcgcaaaacc tatgaaatta aacccgatga acagttcctg ttccacgcag
    13741 cggctggcgg cgttggctta attgcctgcc agtgggcaaa agccctgggc gcgaaactta
    13801 tcggcaccgt aggaaccgcg caaaaagcgc agagcgcgct aaaagcgggc gcgtggcagg
    13861 ttattaacta tcgtgaagag gatctggtcg agcggttaaa agagatcacc ggcggtaaga
    13921 aagtgcgcgt ggtgtacgat tccgtgggca gagacacctg ggaacggtcg ctggattgcc
    13981 tgcaacgccg cggcttaatg gtcagttttg gcaactcatc aggtgcggtt accggtgtga
    14041 acttaggcat tctcaatcaa aaaggctcgt tgtatgtgac acgcccttcc ctgcaaggct
    14101 atatcaccac gcgggaggaa ttaaccgagg ccagtaatga actgttctct ttgattgcca
    14161 gcggtgtgat taaggtcgat gtcgccgagc agcagaaata tccgctgaag gatgcgcagc
    14221 gtgcgcatga gattctggaa agccgggcga cgcaaggttc cagcctgttg attccataaa
    14281 agaaataggg cttccacctg ggaagccctt tctttttata gttcggctgt atgtagggta
    14341 cagcacgatg aatctgttag aggcgcaata gtgacagatt tgattatcaa ttcctatttt
    14401 gttctaagga taaaacctta ggttgtgatc atccgcacaa tcccttagta acgccagcgg
    14461 tcataacgct gatatttcgg cacttttggt gctttaatcg ccttaataac ccacaccacc
    14521 gcaatcgcca gtagtaacca cggcagcaac ttaatcatca atgccagcat accgccgagg
    14581 aacataatgg ccgtcgccac aaccagcgcg gcgataatgc ccagcaacga aacgccggtg
    14641 accatcagca tgacaaaaaa gccaatcaca aaaagtagtt ccagcatgat gctctcccaa
    14701 atatgaaatc tcttgctggc attacaagaa tcatgccaaa aataatctat tgatttaaca
    14761 gcaaaacgcc ccgcgacggt gcgcagggcg tggtgaattt gactactttt tggtgaaaag
    14821 ttaacgctta tccgccacca gtttgagcgc gtgttccagc acattaatgt ctgcacccgc
    14881 tttatgggca ttttcactta aataacgccg ccactgccgc gcgccaggaa taccctggaa
    14941 caagcccaac atatgccggg taatatggcc gagatacgtc ccctggctga gttcacgctc
    15001 aatgtacgga tacatggcgc gcactaccgc caccggatcg gcatcggtat ccgaggaacc
    15061 aaagatctcc cggtctaccg ccgccagaat acccggattc tgatacgcct cgcgcccgac
    15121 catcacgcca tccatatgtt gcaggtgtgc tttggcctct tccagcgact tgataccacc
    15181 gttaatcgac attgtcagat gcggaaagtc acgcttcagt tgatacacac gcggataatc
    15241 gagcggcggg atttcacggt tttctttcgg gcttaacccc gaaagccagg ctttacgtgc
    15301 gtggatgata aacatctcac actcgccttt gccggaaacg gtgttgatga aatcgcagag
    15361 aaattcatag ctgtcctggt catcgatgcc aatacgcgtt ttcaccgtca ctggaatcga
    15421 caccacatcg cgcatcgctt tcacgcagtc ggcaaccagc tgcgcattac ccatcagaca
    15481 cgcaccaaac atgccgttct gcacccggtc agacgggcag ccgacattca ggttgatctc
    15541 atcatatcca cgcgcttctg ccagcttcgc acactgtgcc agcgccgccg gatcgctacc
    15601 gccgagttgc aacgctaccg gatgttcttc ttcactgtac gccaggtaat cacctttacc
    15661 gtgaataatc gcccctgtgg tcaccatttc ggtatacagc aacgtattgc gggaaagcag
    15721 acgcaagaaa tagcggcaat gtctgtccgt ccagtcgagc attggtgcaa cgctaaaccg
    15781 accactccag tgaacgtcag ttttttcagg cattgcgctg gtttggttga ttttttgcat
    15841 ttcagaatta ccgtgcattt tcaaatgtag agattatttt atcgatatat catggggtta
    15901 tgttattcag catcactatt caggaggctc aatagcgggg tactatacca taacaacagg
    15961 aagcgcctgt ctcattgcaa aagaaaattg agatcctctc aaggcatgaa gctctcacga
    16021 agtgatggaa ataatcttat tagccgttaa ctttgttaag gccaatgata aacaatccag
    16081 gttcgacgat aaataaaaaa tcacacatta aactctggtg atatatctcc ctgctaatag
    16141 cattgataga gaaaaaagaa cccaataaat attgggtcct tttatataat gccttccata
    16201 ctatcgaaga acttcacata ttatttctcc gatttaaccc cgaacaaatc ataaattaat
    16261 ttagaagtgt ctgtaagtat tttaatctct tcctttgagg ttgggtcaaa cgacttcgcg
    16321 aagctaatta attgtggtgc agcatctcgc attttgctta aaatatcagg atcgagcgtt
    16381 ccttcattca ccagatgtga catcttatcc agatagccat caaaattcat ttctccccca
    16441 tcagccagac gcttcatctc tcccaggtac ttcttcatat cacgttgaga taatttttca
    16501 aactgagctt tcaggtagct ctcattattt tcattaatat acactgtttc tgataaatct
    16561 gcgtaggcac tagaatatga aagtgaaatt aacagtgaag caaataattt taagctgttg
    16621 tcatcatgtt cctggcaggc attaacaaac gttaaaaaac cagaacctat tgcttgaaaa
    16681 tgtatattcg ctaaaggttc attatcctta gattcctcat aaaatagctg aaccgtggag
    16741 ggaaggccat tcttagtttc tacatcaaaa gtacattgtt caatgggagt cgcatcttta
    16801 ttatccccag gattacatat actattaatc gcgaaatgaa aaatagcccg gtcaacaact
    16861 gatgcaataa agtcacgatc gtttaaatcc tcatcagtgc aatcgtcaat atacttattg
    16921 acccacattt tgagatcatt tatttgctca attaaaaaca cattttttta cgtgcatgcc
    16981 ataaattatg cgtgattcgt tgaataaaca aatctatttt tgcaataatt atatcccgat
    17041 gacgcagcgc tgcataatca atagatttac catatatatt atttaattta cctttgattg
    17101 aacctctatt atctaattta tctttgattg catcttcatt atcgaattga tttgaattga
    17161 gtgttttatt aaagtatcca gttgcaccgg gtattatctt caacatgatt catctacctt
    17221 ttttgtcgta ccgtgtcctc cttataattc ttttgccatc tggtcactat caaaaatcgc
    17281 tcataatttc tgaactacag gtatgcgttt aaacccagtt aaataaatag cgagttaact
    17341 tgaagcaatt tatttaaaaa tgaattatta attattatcg tgttgataat ggtacgacaa
    17401 ggcaacataa caccttacct acaaaacaga aaatcaacat tacggcaaca ataagggttc
    17461 tcggtgtttt tcctggaaca tggtaaagta aggacattct taacccccac tttgaggtgc
    17521 ccgatggaaa agaccacaac gcaggagtta ttagcgcagg ctgaaaaaat ctgcgcgcag
    17581 cgtaatgtgc gcctgacccc acagcgcctg gaagtgttgc gcctgatgag tctgcaggat
    17641 ggcgctatca gcgcttatga tctgcttgat ttactgcgcg aagctgaacc gcaagccaag
    17701 ccgccaacgg tttatcgcgc gctggatttt ctgcttgagc aaggttttgt gcataaggtg
    17761 gaatccacca acagttatgt gctctgtcat ctgttcgacc agcccacgca tacgtcagcc
    17821 atgtttatat gcgatcgctg cggcgcggtg aaagaagagt gtgcagaagg cgtggaagac
    17881 attatgcata cgctggcggc aaaaatgggg tttgccctgc ggcataatgt gattgaagca
    17941 catgggctct gtgcggcatg cgtggaagtg gaagcgtgtc gtcatcctga acagtgccag
    18001 catgatcact ctgtgcaggt gaaaaagaaa ccgcgttaag agggcgtaca tccttgtaca
    18061 cgtcgggcag gagggattaa ttaccagcga tattcattgc gggtttccca atccacgacc
    18121 tctttttctg cctgatcttt ctgataacca taacgttcct ggattttacc gaccagttga
    18181 tcacgtttac cttcaatgat cgtcatatca tcatcggtca gtttgcccca ttgctctttc
    18241 actttacctt taaactgttt ccagttaccg ccggcttcat ctttattcat aatcaagacc
    18301 tcatcgttag gttgtgaatg agagtacgtt cacttttctt ctgaacgtga gattaagtgt
    18361 agtcagcaat ttggcttagg ataatttatt cagaattttt aaccgtcacg ttgcggcaaa
    18421 ccaggtaccg ttacgccagt gacgccgcca gatagccgcc agagaaagcc cgcgcaacgc
    18481 cagaaagacg gttagtgcca gccacaaacc atgattaccc agccacggca gcgtaaggag
    18541 cgtcagcgca aaacctgcgg cggccaccgc catactgtta cgcatttcgg cggcacgcgt
    18601 tgcgcctata aacatgccgt ccagcagata acaccagacg ccaaccaacg gcaaaatcac
    18661 ctgccagata agatagcggt cagccagctg ctgaatctgg gttaacgacg tcagcaacgc
    18721 aatgatgtgt tcccctgcca gcaaataaac caccgaaaat agtaacgcta cgatccccga
    18781 ctggcggcac gctgcccgcc agacatccag caactggcta ccgtcgcgcg caccgtatgc
    18841 ctgaccggaa tgtgcttcaa ccgcgtaggc aaaaccatcc agcgcatagg cggtaaaggt
    18901 gagtagcgtc atcagaaccg cgtttacagc gataatgtca ctccccagtc gcgcgccaag
    18961 tacggtgatc gcgccgaaac agagttgcaa caacagcgaa cgcagcatga tatcgcggtt
    19021 aagcgccagc aagcgacgga agtttcctcg ccaggcagtt ttcgacattt cgccggaaat
    19081 tccgcgtagt ttgaggattt tacgcaccat tagcagacca atcagcaatg ttgcatattc
    19141 cgcaataacc gtcgccagag ccgcgccctg cacgttcata tgcagcccca tcactagcca
    19201 gacatccagc acaatgttga ggatattgcc gaccactaac aaaattactg gcgcacgggc
    19261 atattgcacg ccgagtaacc aaccgagtaa taccagattc gccagcgacg ccggtgcgct
    19321 taaccagcgg atttcaagaa agcgccgcgc ctgttccagg actgcttcac taccgccaac
    19381 aatatgcaga gccagatcga taatcggtgt acgcagcagc gcaattaacg ccccagcccc
    19441 caacgccaac agcaacggtt gcaccagcgc acgggctaat gcctgaggat ttttggcacc
    19501 ataagcctgc gcagtcagcc cggtggtgct catgcgtaaa aacagcaaca gcataaagag
    19561 aaagctggtc gccgttgcac caaccgccac gccgcccaaa taaaccgggc tatcaagatg
    19621 accaattacc gccgtatcga ccagtcccag caacggaacg gtgatattgg agaaaatcat
    19681 gggtaaggcg agatgccaga gtgctttatc agatgaagtg aggaatgcca tgcagacaag
    19741 cctgatgaag agagatgaaa aacaaaccgc gacgcctggc ggcatcgcgg tctcagagat
    19801 atgttacagc cagtcgccgt tgcgaataac cccaaccgcc agcccttcaa tggtgaagct
    19861 ctgctgacga aggtcaacga caattggttt aaactcgcta ttttctggca acagttcgac
    19921 tttattaccc tgttttttca ggcgcttaac ggtaacttcg tcatcaatac gtgcgacaac
    19981 gacctgaccg ttacgtacat cctgagtttt atgcactgcc agcaagtcac catccataat
    20041 gccgatatct ttcatcgaca tcccgctgac gcgcagcagg aaatcagcat tcggcttgaa
    20101 caaggaagga tcgacctgat aatgaccttc aatatgctgc tgcgccagaa gcggttcacc
    20161 ggcagccaca cgacctacca gcggcaaccc ttcttcctct tcctgcaaca gacgaatccc
    20221 gcgtgatgcg ccggaaacaa tttcaataac gcctttgcgt gccagcgcct tcagatgttc
    20281 ttcagccgcg tttggggaac ggaaccccaa acgctgcgcg atttccgcac gcgtcggcgg
    20341 catacctgtc tggctgatgt gatcacggat gagatcaaac acctcttgtt gcctggccgt
    20401 taacgctttc attccgcccc ctgggtgtat atacagttat gctgtgagta tatacagcaa
    20461 aaggcgattt tggaaccata aactgcacaa taaaccagag atttatcgaa atctggaagg
    20521 gttatccaaa atgcgaccat aacaggatgc accaggttat cacggcgacg ataatcgcaa
    20581 tcagcaccgc ggcggatccc atatctttag cgcgtccgga aagctcatgg tattcagagc
    20641 caattcggtc aaccactgct tcgatggcgc tattgagaat ttccacaatc atcaccagca
    20701 tcacggagct gataagcagc acgcgggtaa ttgcgtccac atccagccag caggcgatga
    20761 ccaccgccaa caataccgct acgccttcct gacggaatgc cgcttcgttg atccatgcag
    20821 cgcgtaaacc tttccaggaa tagccagcag ctttgataat tcgggtgaat ccagtggtat
    20881 tattggccat tgaaagaacc tttttacatt atgagcgtca atatcagtgt accgttaaat
    20941 ccgatgattc gggtttgcgt aacgccgcgc gaaacatgag cggataccac agaatttccc
    21001 atgactttct gctatccttg ccgcgcattt gcattattaa ccagaggctt tacatcgttt
    21061 atgtccggct ggccacgaat ttactacaaa ttactgaatt taccattaag catcctggta
    21121 aaaagcaagt ctattccggc agatcctgcc ccggaactgg ggctggatac ctctcgtcca
    21181 attatgtacg ttttaccgta caactcgaaa gcagatttgc tgacgttacg cgcccagtgt
    21241 ctggctcatg acttgcctga cccgttagag ccgctagaaa tcgacggcac gctactgccg
    21301 cgctatgtgt tcattcacgg cgggccgcgt gtattcacct attacacgcc gaaagaagag
    21361 tctattaagc tgttccacga ctatctcgat ttgcaccgta gcaacccaaa tctggatgtg
    21421 cagatggtgc cagtatcggt gatgtttggt cgcgcgccgg ggcgtgaaaa aggcgaagta
    21481 aacccgccgc tgcgtatgct taacggcgta cagaaatttt tcgccgtact gtggctcggt
    21541 cgcgacagtt ttgtgcgttt ctcgccgtca gtttcgctgc gccgtatggc ggatgaacac
    21601 ggcacggata aaactatcgc acagaaactg gcgcgcgtgg cgcgtatgca ctttgcccgt
    21661 caacgtctgg ctgccgtagg cccacgtctg cctgctcgtc aggatctgtt taataagctg
    21721 ctcgcctccc gcgccattgc caaagcggta gaagatgaag cgcgcagcaa aaaaatctcc
    21781 catgaaaaag cgcagcagaa cgcgattgcg ctgatggaag agattgcggc gaatttctct
    21841 tacgagatga tccgcctgac tgaccgcatt ctgggcttca cctggaaccg actttatcag
    21901 ggcatcaacg tccataacgc cgagcgcgtt cgccagctgg cccacgacgg tcatgagctg
    21961 gtgtatgtgc cttgccaccg cagtcacatg gactacctgc tactttctta cgtgctgtac
    22021 caccaggggc tggtgccgcc gcatatcgcc gccgggatca acctgaactt ctggccggcc
    22081 gggccgattt tccgccgtct gggggcgttc tttattcgcc gtacttttaa aggcaataaa
    22141 ctttattcca ccgttttccg tgagtatctc ggcgaactgt tcagccgtgg ttattccgtc
    22201 gagtacttcg tggaaggcgg tcgttcccgc acggggcgtt tgctggatcc gaaaaccggt
    22261 acgctgtcga tgaccattca ggcgatgctg cgtggcggca cgcgtccgat tacgctgatt
    22321 ccgatctata tcggttatga gcacgtcatg gaagtgggta cttacgccaa agaactgcgc
    22381 ggcgcgacga aagagaaaga gagcctgccg cagatgctgc gcggtttaag caagctgcgt
    22441 aatctcggtc agggttacgt caacttcggt gaaccaatgc cgttgatgac ctaccttaac
    22501 cagcacgtac cagactggcg tgaatctatc gatcccatcg aagcggtgcg tccggcctgg
    22561 ttaacgccga cggtcaataa tattgctgcc gatctgatgg tacgcattaa caacgcaggc
    22621 gcggcaaacg ccatgaacct gtgttgtact gcgcttctgg catcacgtca gcgctcactc
    22681 acccgcgagc agttaaccga gcaactcaac tgctacctgg atctgatgcg caacgtacct
    22741 tactccacgg actctaccgt tccttcagcc agcgccagcg agcttatcga tcacgcgctg
    22801 caaatgaaca agtttgaagt cgagaaagac actatcggcg acatcatcat tctgccgcgc
    22861 gagcaagcgg tgctgatgac ctactatcgc aacaacattg cgcatatgtt ggtgctgcct
    22921 tcgctgatgg cggcaattgt cacccagcat cgccacatct cccgcgacgt attgatggag
    22981 cacgtcaatg tgctttaccc aatgctgaaa gcggagctgt tcctgcgctg ggatcgcgac
    23041 gagttgccgg acgttattga tgcgctggca aatgagatgc aacgtcaggg gctgattacc
    23101 ctgcaagatg atgagttgca tatcaacccg gcgcattctc gccccctaca gctgctggcc
    23161 gcaggcgcgc gcgaaacgct gcaacgttat gccatcacct tctggttgtt gagtgccaac
    23221 ccgtcgatca accgcggtac gctggagaaa gagagccgca ccgttgcgca acgtctctcc
    23281 gtgctgcacg gcatcaacgc gccggagttc ttcgacaagg cggtgttcag ttctctggtg
    23341 ctgacgctgc gtgatgaagg gtatatcagc gatagcggcg acgccgaacc ggcagaaacg
    23401 atgaaggttt atcagttgct ggcggagttg attacatcag acgtgcgttt gacgattgag
    23461 agtgcgacgc agggcgaagg gtaatcagag agaattgccg gatgcggcga aaacgcctta
    23521 tccggccttc cataatctgc aaattcaata aattgcgatg tgttgtgtag gcctgagaag
    23581 cgcagcgcat caggcaaccc agaagaaagc cggatgatca tccggctttt ttacatcatc
    23641 agaaatgcca gtaactcatt gccagtccta aaaacagtac cagaccaaca tagttattat
    23701 tcataaatgc tttaaagcag gcttcacgct cgcggttggc aatcagtttt tgttgataaa
    23761 caaacagcgc gccagccacc agaattgacc agtaatatcc ccagcctaag ccatttaact
    23821 caccgatgat cgccatcagt gccagtacgc caatctgcaa aataccaata atcaatttat
    23881 cgtattggcc gaacaggatt gcggtggatt taataccaat cttcacatca tcgtcacggt
    23941 caaccattgc atactgcgtg tcgtaagcca ccgcccagag aatattggcg aggaacatta
    24001 accagcaact caatggcacc gactcactca cagcggcaaa agccattgga atcgaccagc
    24061 caaacgccgc gcccagcacc acttgcggga gatgggtata ccgcttcata aacgggtaca
    24121 cccacgccaa cgccagagcg gcaatcgaca acagaatggt catcgtattc agcgtcagca
    24181 ccagtaaaaa tgaaatcaat accagcacta caaacagcgc gcgcgcctct ttctccgtta
    24241 ccgcgccgct gggaagcggt cggttcgccg tgcgcttaac atgaccatca aacttgcggt
    24301 cagcataatc attcaccaca catccggcag cgcgcatcag ccagacaccc gcgacaaaca
    24361 ccgccaggat ccagagctgg ggaacgcccg gtgtcgccac ccacaacgcc cataatgttg
    24421 gccagagcag cagtaacgcg ccaattggct tatccgtacg cattaagcga tgaaacgcca
    24481 gcagcttatt ctgcgtcaga ctccactcca tattttttcc tcttagtaca acggtgacgc
    24541 cggtaaaaac agttctgtta gcaacagcgg tttaccgctt aatcgcaggc gggaacgtcg
    24601 cccccacagt ccggcatcac ggcctatctc aataaagtcc cgggttaatg tcgatgatgt
    24661 gaacagatag cgtcctagcg gcgttttacc caatttttgt aacgccagct ccggcccgct
    24721 taacgttgac acaggaacga cggtacgacc ggcaagccac ggttcaccat cggcacataa
    24781 caaaatttca cgtaaccagt aacgagactc tttcggcagc agcggcagtt cttcggggat
    24841 ttcattctgc tcgacaaacc cttcgcggat catcgtcacg ctcaccgttt ttccctgctg
    24901 ttcaaaacgt tttgtcatgg aatcttccag caacagccag tcgagcagtt gcgaatccag
    24961 ggcagggatc tctttaaaat agcgcagcgc acgcagttgc gttaacgcgg ggtgtgacat
    25021 gccgaactct ccgttacata acgtaaaggc attgtatcgc agaaaagtgt ttacgggaga
    25081 tgtaaagcaa attaagtgat ctaatgcgca acaaaaacgc aacaatcgca tgaagtggta
    25141 aaacgctgtt gaaccttacg ctttgccgat gcccgtgctg gcgagggcaa ccgtaggggc
    25201 atcaccgccc atgatgccag gaacggtgat gttttccttt acttaaatac ctatattctt
    25261 tatacgagta gctcgccaac tcgcagtaaa aattgggaaa agcgcatatt taccatgaga
    25321 tttggagcta aaaagtgttt cctgagtaag attaataggc aacatcttat gtctggattt
    25381 atagatacac gtggaattcc aattggtatc ttttgtagga ttcaacatat catgtaaggc
    25441 agtatgactt tccgtgagac agatgctggc caacgtggga tatttaacgc gggttaacat
    25501 cgttgtcaga gccgcatggg tgagctgttg ctacaccccg gtatttccac cacggaggcc
    25561 agcatgaaca acaataacac attgtacgtc gggttagatg ttcacaaaga atcgattact
    25621 gtcgcttatg ctatcaattc agaacctgtt gaactgatgg gtaaaattgg cacatcacct
    25681 actgatattc agaatctttg taaacgtctc aggtcaaagt catc
//
LOCUS       DAJTVK010000052        25647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000052 DAJTVK010000000
VERSION     DAJTVK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.052"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OO372_002179"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="OO372_002179"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6341667.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OO372_002180"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="OO372_002180"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6341668.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OO372_002181"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="OO372_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6341669.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OO372_002182"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="OO372_002182"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6341670.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="OO372_002183"
     CDS             complement(5499..5696)
                     /locus_tag="OO372_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6341671.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OO372_002184"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="OO372_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6341672.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OO372_002185"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="OO372_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6341673.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OO372_002186"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="OO372_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6341674.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OO372_002187"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="OO372_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6341675.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OO372_002188"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="OO372_002188"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6341676.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OO372_002189"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="OO372_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6341677.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OO372_002190"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="OO372_002190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6341678.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OO372_002191"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="OO372_002191"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6341679.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OO372_002192"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="OO372_002192"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6341680.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="OO372_002193"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="OO372_002193"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6341681.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="OO372_002194"
     CDS             complement(17138..17191)
                     /locus_tag="OO372_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341682.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="OO372_002195"
     tRNA            complement(17425..17500)
                     /locus_tag="OO372_002195"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="OO372_002196"
     tRNA            complement(17536..17611)
                     /locus_tag="OO372_002196"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="OO372_002197"
     tRNA            complement(17769..17844)
                     /locus_tag="OO372_002197"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="OO372_002198"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="OO372_002198"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6341683.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="OO372_002199"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="OO372_002199"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6341684.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="psd"
                     /locus_tag="OO372_002200"
     CDS             19844..20812
                     /gene="psd"
                     /locus_tag="OO372_002200"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6341685.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="OO372_002201"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="OO372_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6341686.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="OO372_002202"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="OO372_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCR6341687.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5935 a         7194 c         6498 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcat ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DAJTVK010000053        25130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000053 DAJTVK010000000
VERSION     DAJTVK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.053"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(166..576)
                     /locus_tag="OO372_002203"
     CDS             complement(166..576)
                     /locus_tag="OO372_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCR6341688.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="OO372_002204"
     CDS             complement(634..867)
                     /locus_tag="OO372_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR6341689.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="OO372_002205"
     CDS             1256..1801
                     /locus_tag="OO372_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6341690.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="OO372_002206"
     CDS             1776..3701
                     /locus_tag="OO372_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6341691.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="OO372_002207"
     CDS             3698..3904
                     /locus_tag="OO372_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR6341692.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="OO372_002208"
     CDS             3901..5502
                     /locus_tag="OO372_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6341693.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="OO372_002209"
     CDS             5483..6802
                     /locus_tag="OO372_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6341694.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="OO372_002210"
     CDS             6812..7144
                     /locus_tag="OO372_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6341695.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="OO372_002211"
     CDS             7200..8225
                     /locus_tag="OO372_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6341696.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="OO372_002212"
     CDS             8267..8665
                     /locus_tag="OO372_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6341697.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="OO372_002213"
     CDS             8677..9030
                     /locus_tag="OO372_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR6341698.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="OO372_002214"
     CDS             9042..9620
                     /locus_tag="OO372_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6341699.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="OO372_002215"
     CDS             9617..10012
                     /locus_tag="OO372_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6341700.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="OO372_002216"
     CDS             10020..10760
                     /locus_tag="OO372_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6341701.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="OO372_002217"
     CDS             10776..11198
                     /locus_tag="OO372_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6341702.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="OO372_002218"
     CDS             11180..11614
                     /locus_tag="OO372_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6341703.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="OO372_002219"
     CDS             11607..14168
                     /locus_tag="OO372_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6341704.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="OO372_002220"
     CDS             14165..14494
                     /locus_tag="OO372_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6341705.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="OO372_002221"
     CDS             14494..15192
                     /locus_tag="OO372_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6341706.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="OO372_002222"
     CDS             15198..15941
                     /locus_tag="OO372_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6341707.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15878..16510
                     /locus_tag="OO372_002223"
     CDS             15878..16510
                     /locus_tag="OO372_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6341708.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="OO372_002224"
     CDS             16571..20068
                     /locus_tag="OO372_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6341709.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="OO372_002225"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="OO372_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..22124
                     /locus_tag="OO372_002226"
                     /pseudo
     CDS             20720..22124
                     /locus_tag="OO372_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            22073..22225
                     /locus_tag="OO372_002227"
                     /pseudo
     CDS             22073..22225
                     /locus_tag="OO372_002227"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            22202..22381
                     /locus_tag="OO372_002228"
                     /pseudo
     CDS             22202..22381
                     /locus_tag="OO372_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23096..23803
                     /locus_tag="OO372_002229"
                     /pseudo
     CDS             23096..23803
                     /locus_tag="OO372_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            23803..24384
                     /locus_tag="OO372_002230"
     CDS             23803..24384
                     /locus_tag="OO372_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6341710.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         6040 a         6197 c         7555 g         5338 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagcgggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgtggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgctt
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatcctgtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctatgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gtgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcacagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacacc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacagtac cgatattgca
    23941 ccgccagata ttccggctgg tttcgtggct gttttcaaca gtgatgagtc atcgtggcat
    24001 ctcgttgaag atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt
    24061 atttctgaac tcggctcatt accggaaaat gtcacctggt tatccccgga aggggagttt
    24121 cagaagtgga acggcacagc ctgggtgaag gatacggaag cagaaaaact gttccggatc
    24181 cgggaggcgg aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg
    24241 cttcaggatg ctgtggatct ggagatcgca acggagaaag aaaccttgtt gctggaagct
    24301 tggaaaaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc ggatattgaa
    24361 tggccagtag cacctatagg gtaaattcgt aatgattacc taaatacgtt attcttttgt
    24421 taaaaaagtg attttgctat aggtagggag tatgttcttg gattaagaat aaaaaatcgc
    24481 catgaaagag cttcatggcg agtacataaa cgtacaaaat gtgatgaaag tagagtggcg
    24541 taagccacta tattaagata tgacaatagc gaagaaaagt aaataacatt ttatggacgt
    24601 ttaattgtta attataaaaa ctagagtgcc cgatattgtt tatcttgctt taaaataatg
    24661 tactcaaacc aaatttttga gctaaaagct caaataatat aaatgcaata ataatgttta
    24721 ataagacaat tagcttgctt tgtattttca tttcggaata ctgttaagta actttgatga
    24781 ccctaacaat agatcggaat tttcttagag tcaatggtca ctgtttttgc ttgcaaagca
    24841 aatgttttgg tggttataag tcgtcttaat atagtcacgc atgcaacttg ttccatgcgg
    24901 agattaaaga ttggaatata acgcgaagat ggaattaaat tacatttgca ataattaatt
    24961 tattattact caataattac cgatattatt attgtaaatt aatcattaga atgcaaaaag
    25021 aaatgtaacg gcaaaataag aggacgatag ctgagaaaaa agcgtgcttt cccaatctgg
    25081 ggcaaatcac gtctgaatga cacctgaaaa caggtggtag cctcaagaag
//
LOCUS       DAJTVK010000054        25105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000054 DAJTVK010000000
VERSION     DAJTVK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.054"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            196..384
                     /locus_tag="OO372_002231"
     CDS             196..384
                     /locus_tag="OO372_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6341711.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="OO372_002232"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="OO372_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6341712.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="OO372_002233"
     CDS             1145..1309
                     /locus_tag="OO372_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6341713.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1502..3763
                     /gene="bcsA"
                     /locus_tag="OO372_002234"
     CDS             1502..3763
                     /gene="bcsA"
                     /locus_tag="OO372_002234"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR6341714.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3774..6112
                     /gene="bcsB"
                     /locus_tag="OO372_002235"
                     /pseudo
     CDS             3774..6112
                     /gene="bcsB"
                     /locus_tag="OO372_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            6119..7225
                     /gene="bcsZ"
                     /locus_tag="OO372_002236"
     CDS             6119..7225
                     /gene="bcsZ"
                     /locus_tag="OO372_002236"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6341715.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7207..10699
                     /gene="bcsC"
                     /locus_tag="OO372_002237"
                     /pseudo
     CDS             7207..10699
                     /gene="bcsC"
                     /locus_tag="OO372_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10781..12769
                     /gene="hmsP"
                     /locus_tag="OO372_002238"
     CDS             10781..12769
                     /gene="hmsP"
                     /locus_tag="OO372_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6341716.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12952..14238
                     /gene="dctA"
                     /locus_tag="OO372_002239"
     CDS             12952..14238
                     /gene="dctA"
                     /locus_tag="OO372_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6341717.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14458..15954
                     /locus_tag="OO372_002240"
     CDS             14458..15954
                     /locus_tag="OO372_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6341718.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16050..16979)
                     /gene="kdgK"
                     /locus_tag="OO372_002241"
     CDS             complement(16050..16979)
                     /gene="kdgK"
                     /locus_tag="OO372_002241"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6341719.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17211..17978
                     /gene="pdeH"
                     /locus_tag="OO372_002242"
     CDS             17211..17978
                     /gene="pdeH"
                     /locus_tag="OO372_002242"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6341720.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18048..20108
                     /locus_tag="OO372_002243"
     CDS             18048..20108
                     /locus_tag="OO372_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6341721.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20290..21612)
                     /locus_tag="OO372_002244"
     CDS             complement(20290..21612)
                     /locus_tag="OO372_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6341722.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22023..23036)
                     /gene="yhjD"
                     /locus_tag="OO372_002245"
     CDS             complement(22023..23036)
                     /gene="yhjD"
                     /locus_tag="OO372_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6341723.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISDLH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23085..23984)
                     /locus_tag="OO372_002246"
     CDS             complement(23085..23984)
                     /locus_tag="OO372_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6341724.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24504..>25105
                     /locus_tag="OO372_002247"
     CDS             24504..>25105
                     /locus_tag="OO372_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6341725.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6365 c         6990 g         5855 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tgaggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcggctgcc
     4201 agtgaccaaa gaacagttgg gtaaaaaaac gctggcgcaa atgcccatta acccactgtt
     4261 tattaccgac ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga
     4321 aaacccggcc agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac
     4381 ctatcagacc ctgaatgtga agagtgacct gtcacacttc ccggtgccat tctttgaccc
     4441 gcgcgataac cgtactaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgggct
     4501 gcaacaagca tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca
     4561 gaacttcccg gtgctctata accaattgcc ggatcgcaat gccattgtct ttgcaaccaa
     4621 cgacaaacgg ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat
     4681 gattaaccat ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa
     4741 agacctgttg caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag
     4801 cgtggtagtg aatgaagtga aaccgctgct accgcgtaag ccgtacgatg cgccgaactg
     4861 ggtacgtacc gatcgtccgg tcacatttgg cgaactgaaa acctatgaag aacagttaca
     4921 atccagcggt cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta
     4981 cctgatgcgc agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt
     5041 gaaagacagt tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct
     5101 gagcagtaaa caggaggcga accgcctgct gctgcggatt ccggtattac aaggtttgct
     5161 ggatggcaaa acggatgtct ctattccggc gctgaaactg ggcgcgacca accagctgcg
     5221 cttcgacttt gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt
     5281 ccagccggtg cagaatcatg tggtgattgg tgacgattcc accatcgact tctcgaagta
     5341 ttaccacttc atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccgttcag
     5401 ccggatggcg gatctgtcgc aaaccatcac cgtgatgccg aaaacgccta acgaagcaca
     5461 gatggaaacg ttgctgaata ctgttggttt tatcggcgca cagacgggct tcccggcgat
     5521 taatctgacg gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat
     5581 catcggtggt atcccggaca aattaaaaga cgataagcag atcgacctgt tggtgcaggc
     5641 gaccgaaagc tgggtgaaaa caccgatgcg ccagaccccg ttccccggca ttgtgccgga
     5701 cgagagcgat cgtgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc
     5761 ggtgattggc ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga
     5821 tagcccacgc ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac
     5881 catgttcggt tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga
     5941 cgtttattac gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca
     6001 tccgattctg ctggcggtgc tggcggcaat cagtgtgata ttgctggcat gggtactgtg
     6061 gcgtctgctg cgaattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat
     6121 gaatgtgttg cgtagtggaa tcgtgacgat gctgctgttg gctgccttta gtgttcaggc
     6181 agcctgtacc tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg
     6241 cgtcatcgcc cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggtat
     6301 gttctttgcc ctggcggcta acgaccgtgt agctttcgat aatattctcg actggacgca
     6361 gaacaatctc gctcagggct ctttaaaaga acgtttgccc gcctggctgt ggggcaagaa
     6421 agagaacagt aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat
     6481 ggcctggtcg ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg
     6541 cagcgcgttg ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggttc
     6601 catgttgtta ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag
     6661 ctacctgccg ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct
     6721 gcgcgaaacc aatcaacgtt tattgctgga aaccgccccg aaaggttttt cgccagactg
     6781 ggtgcgctat gagaaagaca aaggctggca gctaaaagcc gaaaaaacat tgatcagcag
     6841 ctacgacgct atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa
     6901 agcgcggatg ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc
     6961 gccggaaaaa gtggatgtgg ctacggggaa agcgcagggt aaaggaccga tcggtttttc
     7021 tgccgccatg ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt
     7081 ggccgataac tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca
     7141 aggctgggat caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg
     7201 ccaggaatgc gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg
     7261 ccgatggtcg aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc
     7321 gaagcgaccc atcgtgaaga tctggtgcaa cagtcgttat atcggctgga acttattgat
     7381 ccgaataacc cggacgtcgt tgccgcccgt ttccgttctt tgttacgtca gggcgatatt
     7441 gatggcgcgc aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat
     7501 aaatcgtcgc ggactacgat gctactttcc acgccggatg gtcgtcaggc actgcaacag
     7561 gcacgattgc aggcgacgac cggtcatgca gaagaagctg tggcgagtta caacaaacta
     7621 ttcaacggtg cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa
     7681 attccggctc gccgtggcga agcgattaat cagttaaaac gcatcaatgc ggatgcaccg
     7741 ggcaatacgg gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac
     7801 gaaggttttg ccgtcctgga acagatggca aaatcgaacg ccgggcgcga aggggcctct
     7861 aaaatctggt acgggcagat taaagacatg cccgtcagtg atgccagtgt gtcggcgctg
     7921 aaaaaatatc tctcgatctt tagtgatggc gatagcgtgg cggctgcgca atcgcaactg
     7981 gcagaacagc aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg
     8041 gcggtggact ctggtatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg
     8101 aacccgaaag acagtgaagc tctgggggcg ctgggccagg cgtattctca gaaaggcgat
     8161 cgcgccaatg cagtggcgaa tctggaaaaa gccctcgcac tggacccgca cagcagcaac
     8221 aacgacaaat ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc
     8281 gatgctgcgc tgaaagccaa taatcctgac cgggcagaac gcctgttcca gcaggcgcgt
     8341 aatgtcgata acaccgacag ttatgcagtg ctggggctgg gcgatgtggc gatggcgcga
     8401 aaagattatc ccgccgccga acgttattat cagcagacct tgcgtatgga cagcggcaac
     8461 actaacgccg tgcgcgggct ggcaaatatt taccgccagc aatcgccaga aaaagctgaa
     8521 gcgtttatcg cctcgctctc tgccagtcag cggcgtagca ttgatgatat cgaacgcagc
     8581 ctgcaaaacg accgtctggc acagcaggca gaggcactgg aaaaccaggg caaatgggcg
     8641 caggcggcag cacttcagcg gcaacgactg gcgctggacc ccggcagcgt atggattact
     8701 taccgacttt cgcaggatct ctggcaggcc ggacaacgca gccaggccga tacgttaatg
     8761 cgcaatctgg cgcagcagaa gtcgaacgac ccagagcagg tttacgctta cgggctgtac
     8821 ctacgcttac gggctgtacc tctctggtca tgaccaggac agagcggcgc tggcgcatat
     8881 caacagcctg ccgcgtgcgc agtggaacag caatattcag gagctggtta atcgactgca
     8941 aagcgatcag gtgctggaaa ccgctaaccg cctgcgagaa agcggcaaag aggcagaagc
     9001 ggaagcgatg ctgcgccagc aaccaccttc cacgcgtatt gacctcacgc tggctgactg
     9061 ggcgcaacaa cgacgtgatt acaccgccgc ccgcgctgca tatcagaatg tcctgacgcg
     9121 ggagccagct aacgccgacg ccattcttgg tctgacggaa gtggatattg ctgccggtga
     9181 caaagcggcg gcacgtagcc agctggcgaa actgcccgct accgataacg cctcgctgaa
     9241 cacacagcgg cgcgtggcgc tggcacaggc gcagcttggc gataccgcag cagcgcagcg
     9301 gacgtttaat aagttgatcc cgcaggcaaa atctcagcca ccgtcgatgg aaagcgcgat
     9361 ggtgctgcgt gatggtgcga agtttgaagc gcaggcgggc gatccaacgc aggcgctgga
     9421 aacctacaaa gacgccatgg tcgcatccgg tgtgactacg acgcgtccgc aggataacga
     9481 cacctttacc cgactgaccc gtaacgacga gaaagatgac tggctgaaac gtggcgtgcg
     9541 cagcgatgcg gcggacctct atcgccagca ggatcttaac gtcacccttg agcacgatta
     9601 ctggggttcg agcggcaccg gtggttactc cgatctgaaa gcgcacacta ccatgttgca
     9661 ggtggatgcg ccgtattctg acgggcggat gttctttcgc agtgatttcg tcaatatgaa
     9721 cgtcggcagt ttctccacta atgccgatgg caaatgggat gacaactggg gcacctgtac
     9781 attacaggac tgtagcggca accgcagcca gtcggattcc ggtgccagcg tggcggtcgg
     9841 ctggcgaaat gacgtctgga gctgggatat cggtaccacg ccgatgggct tcaacgtggt
     9901 ggatgtggtc ggcggcatca gttacagcga tgatatcggg ccgctgggtt acaccgttaa
     9961 cgcccaccgt cggcccatct ccagttcttt gctggccttt ggtgggcaaa aagactcccc
    10021 gagcaatacc gggaaaaaat ggggtggcgt acgtgccgac ggtgtggggc taagtctgag
    10081 ctacgataaa ggtgaagcaa acggcgtctg ggcatcgctt agtggcgacc agttaaccgg
    10141 taaaaatgtc gaagataact ggcgcgtgcg ctggatgacg ggctattact ataaggtcat
    10201 taaccagaac aatcgccgcg tcacaatcgg cctgaacaac atgatctggc attacgacaa
    10261 agatctgagt ggctactcac tcggtcaggg cggttactac agtccgcagg aatacctgtc
    10321 gtttgccata ccggtgatgt ggcgggagcg cacggaaaac tggtcgtggg agctgggggc
    10381 gtctggctcg tggtcgcatt cacgcaccaa aaccatgccg cgttatccgc tgatgaatct
    10441 gatcccgacc gactggcagg aagaagctgc gcggcaatcc aacgatggcg gcagcagtca
    10501 gggcttcggc tacacggcgc gggcattact tgaacgacgt gttacttcca actggtttgt
    10561 tggcacggca attgatatcc agcaggcgaa agattacgca cccagccatt tcctgctcta
    10621 cgtacgttat tccgccgccg gatggcaggg tgacatggat ttaccgccgc agccgctgat
    10681 accttacgcc gactggtaag ttttcagata gcgcctctct taatgccgct gcgatcgggt
    10741 atactcgggc ggcaatctgg gatttccggg gggagacaat ttgcgcgtaa gtcgctcgtt
    10801 aacaatcaag cagatggcaa tggtggcagc cgttgtcctg gtgttcgttt ttattttttg
    10861 caccgttttg ctgttccatc tggtccagca gaatcgctat aacacggcta cgcaactgga
    10921 aagcattgct cgctctgtcc gcgaaccctt atcttcagct attttgaaag gcgatattcc
    10981 cgaagcggaa gctattcttg ccagcattaa accggcaggc gtggtcagcc gtgccgatgt
    11041 agtgctgcct aaccagttcc aggcgctgcg taaaagtttt attccagagc gcccggtgcc
    11101 gataatggtt actcgcctgt ttgagctacc ggttcaaatc tcgctgggcg tttactcgct
    11161 cgaacgtccg gcaaacccgc agccaattgc ctatctggta ctacaggcgg attccttccg
    11221 tatgtataag ttcgtgatga gcaccctctc aacgttagtg accatttact tacttttgtc
    11281 gcttatcctg accgtcgcca tcagctggtg cattaaccgc ctgattttgc atccgttacg
    11341 caatattgct cgcgaactta acgccatccc agcccaggag cttgttggtc accaactggc
    11401 attaccgcgt ctgcatcagg acgatgaaat cggtatgttg gtgcgcagtt acaacctcaa
    11461 ccagcaattg ctgcagcgcc attatgaaga acagaacgaa aatgcgatgc gcttcccggt
    11521 gtcggatttg ccgaacaaag ccttgctgat ggagatgctg gagcaggttg tcgcgcgtaa
    11581 acaaaccacc gcgctgatga tcatcacctg tgaaaccctg cgtgatactg cgggcgtgct
    11641 gaaagaggcg caacgagaaa ttctgctgct gacgctggtg gaaaaactca aatcggtact
    11701 gtcgccacgt atgatcctcg cgcagattag cggttatgac tttgctgtca ttgccaacgg
    11761 tgtacaggaa ccgtggcacg caatcacctt aggtcagcaa gtgctcacca tcatgagcga
    11821 gcgcctgccg attgaacgta ttcaactccg tccgcactgt agcattggcg tggcgatgtt
    11881 ctacggcgat ctcaccgccg aacagcttta cagtcgcgct atttctgcgg catttaccgc
    11941 tcgccataaa ggcaagaatc agattcagtt ctttgatccg cagcagatgg aagccgccca
    12001 gaagcggttg acggaagaga gcgatatcct taatgcactg gaaaatcatc agtttgctat
    12061 ttggttacag ccacaggtcg agatgaccag cggtaaactg gtcagtgcgg aagtgttact
    12121 gcgtatccag caaccggatg gcagttggga cctgccggat ggcttaatcg atcgcattga
    12181 gtgctgtggg ctgatggtta ccgtcggtca ctgggtgctg gaagagtcct gtcgattgct
    12241 tgcagcctgg caagagcgcg gcattatgct gcccttgtcg gtaaacctct ctgcgctgca
    12301 actgatgcac ccaaatatgg tggcggatat gctggaactg ttaacccgct atcgcattca
    12361 gccgggaaca ctgattctgg aagtgacaga aagccgacgt attgacgacc ctcatgctgc
    12421 ggtggcaatc ctccgtccgc tgcgcaatgc cggagttcgg gtggcgctgg atgatttcgg
    12481 catgggctac gcagggctgc gtcagctgca gcatatgaaa tcgttgccaa tcgacgtact
    12541 gaaaatcgac aaaatgtttg ttgaaggctt gccggaagat agcagcatga ttgctgcaat
    12601 tatcatgctg gcgcagagcc tgaacttaca aatgattgcc gaaggcgtgg agactgaagc
    12661 acaacgcgac tggctggcaa aagcgggcgt tggtattgcc cagggcttcc tttttgctca
    12721 cccactccct attgaaatct tcgaagagag ttacctggaa gaaaagtagc taccccaaac
    12781 tgattacaaa actttaaaaa gtgctggttt gtgcgagcca gctcaaactt tttaaccttt
    12841 ttgtttcaat tatgatccag gtacatttct gtgatgttgt ctgggtgtta ttttaaggcc
    12901 gcaggtaccc cataacctta caagacctgt ggttttacta aaggacaccc tatgaaaacc
    12961 tctctgttta aaagccttta ctttcaggtc ctgacagcga tagccattgg tattctcctt
    13021 ggccatttct atcctgaaat aggcgagcaa atgaaaccgc ttggcgacgg cttcgttaag
    13081 ctcattaaga tgatcatcgc tcctgtcatc ttttgtaccg tcgtaacggg cattgcgggc
    13141 atggaaagca tgaaggcggt cggtcgtacc ggcgcagtcg cactgcttta ctttgaaatt
    13201 gtcagtacca tcgcgctgat tattggtctt atcatcgtta acgtcgtgca gcctggtgcc
    13261 ggaatgaacg tcgatccggc aacgcttgat gcgaaagcgg tagcggttta cgccgatcag
    13321 gcgaaagacc agggcattgt cgccttcatt atggatgtca tcccggcgag cgtcattggc
    13381 gcatttgcca gcggtaacat tctgcaggtg ctgctgtttg ccgtactgtt tggttttgcg
    13441 ctccaccgtc tgggcagcaa aggccaactg atttttaacg tcatcgaaag tttctcgcag
    13501 gtcatcttcg gcatcatcaa tatgatcatg cgtctggcac ctattggtgc gttcggggca
    13561 atggcgttta ccatcggtaa atacggcgtc ggcacactgg tgcaactggg gcagctgatt
    13621 atctgtttct atattacctg tatcctgttt gtggtgctgg tattgggttc aatcgctaaa
    13681 gcgactggtt tcagtatctt caaatttatc cgctacatcc gtgaagaact gctgattgta
    13741 ctggggactt catcttccga gtcggcgctg ccgcgtatgc tcgacaagat ggagaaactc
    13801 ggctgccgca aatcggtggt ggggctggtc atcccgacag gctactcgtt taaccttgat
    13861 ggcacatcga tatacctgac aatggcggcg gtgtttatcg cccaggccac taacagtcag
    13921 atggatatcg tccaccaaat cacgctgtta atcgtgttgc tgctttcttc taaaggggcg
    13981 gcaggggtaa cgggtagtgg ctttatcgtg ctggcggcga cgctctctgc ggtgggccat
    14041 ttgccggtag cgggtctggc gctgatcctc ggtatcgacc gctttatgtc agaagctcgt
    14101 gcgctgacta acctggtcgg taacggcgta gcgaccattg tcgttgctaa gtgggtgaaa
    14161 gaactggacc acaaaaaact ggacgatgtg ctgaataatc gtgcgccgga tggcaaaacg
    14221 cacgaattat cctcttaatc tcaccactta tgcccgcact cccttccccg agtgcgggtg
    14281 tttgcgcata attgacccct gtgcctgaac ttaccacctg ggtgagttca ggtttatttc
    14341 acttcttccg tgacattttc atgttcttgc ggtctaacac gaagtgtttt taacgtcatt
    14401 tagactgact gacaacgggc agttttttat taccaggata gttgatcagg ggttcacatg
    14461 cagggcacaa aaattcgact tttagcgggc ggtttgctga tgatggccac tgctggctat
    14521 gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct ttccaacggt
    14581 ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga aattcgcctg
    14641 ctggttaata tcggttcgct cgccgaaagt acacaacaga gcggttacag tcacgccatc
    14701 cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg ttcattgtgg
    14761 cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta tgacaccacg
    14821 ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc gctctcttat
    14881 ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca cgcgctgcaa
    14941 agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg gcgctatcgt
    15001 ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca acccgttgaa
    15061 gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat gacgctactg
    15121 gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac gtttggcgaa
    15181 ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct gcgtgcggaa
    15241 gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat gtgggatacg
    15301 ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc ggacctggcc
    15361 cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtaacag caaagacatc
    15421 ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat caacatcgaa
    15481 tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact ggcgaaggtt
    15541 cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa gaaactggag
    15601 ctgcagaaac tgtttgccgc ctatgcgcgg gctgataccg atattctgat gggtcagcgg
    15661 atgcgttcgc tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca gaaactgcgg
    15721 caggatttcc ttaacagcct gacggtggag atgttaaatc aggatctgcg tcagcagttg
    15781 tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt taacatgaaa
    15841 gcgttgcagg cggcctggga tcaaatcatg gccccatcga ctgctgctgc cgccacctct
    15901 gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca gtaatgcctg
    15961 atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag aggggtaaca
    16021 ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg cgggataatc
    16081 gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc cgcgttttcc
    16141 gcactgccgc ccgtcaaaca taccgccaga taaccggcgc tgaaagagtc gccagctgcg
    16201 gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatccac taacccttcg
    16261 ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc tttcacgccc
    16321 gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag cgcgtcttca
    16381 tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgttggta cacctgctgt
    16441 gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat cacttttccg
    16501 ccgttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt cgggcttaag
    16561 atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc ttcgcaaatc
    16621 gccgcagact gctcactctc cagccagaat ttggcggcgg cttcgttccg ccagtagtag
    16681 aacgtacgct cgccggtgct gtcggtttca atgtagtaaa ggcccggcag acggttttcc
    16741 atccgttggg tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag catttgctgg
    16801 ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc aggatcgacc
    16861 tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg cttaacgtcc
    16921 gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt tttggacata
    16981 gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt ttgctggtca
    17041 accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct gaaagattcc
    17101 gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt gcgcgccgat
    17161 aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg atgataaggc
    17221 aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag gaacggcgtt
    17281 tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca tgcggacggt
    17341 taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg caacgcctgc
    17401 cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt gtgaaagagc
    17461 agattgattt gctggcgcaa aaagccgact tctttgtaga gcacgacctg ctggcatcgg
    17521 tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc ctgcgccaaa
    17581 ttgagcgtct tccctggctg cgtttcgaac tggtggaaca catccgcctg ccgaaagatt
    17641 caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt ggcaccggga
    17701 tggcaaattt ctctgcgcta agtgaagtgc gttatgacta catcaaaatc gcgcgagagc
    17761 tgttcgtgat gctgcgtcag tcgccggaag gccgcacact cttttctcag cttttacatc
    17821 taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacaccg gaagagtggc
    17881 gtgatgttca gaactcgccc gcattcgccg cacaaggctg gtttctttca cgcccggcac
    17941 cgatggaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt tccgctgggc
    18001 tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg agcaaggcag
    18061 gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg gcgatcattt
    18121 tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa gtctctgcgg
    18181 agttgaatcg tccgttcgct atccgtggcg atctgggcgt ggtgtgggag cggcaaaaac
    18241 aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa gacatcattc
    18301 ttggtaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga gtagaggcaa
    18361 cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggttc aagctcgaaa
    18421 agcccgacgc gcgcctgatt cgcctctccg aaaagaacaa taactggacg tttaatcttg
    18481 ccaacgatga caacaaagac gcgaatgcaa agccgtcggc atggtcgttt cggctggata
    18541 atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa gcggatctgg
    18601 aaatttttgt tgatccgtta ggcaagccgc tgccgttcag cgaagttact ggatcgaaag
    18661 gtaaagcgga taaagaaaag gtgggcgatt acgtttttgg cctgaaggcg cagggacgtt
    18721 ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg ctgcgtggcg
    18781 aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt gttgcttttg
    18841 atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt aaattttctg
    18901 gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat accccgccgt
    18961 ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg gtctttgatt
    19021 atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg gtctacacca
    19081 ccggaaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg cggctggcgg
    19141 acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg aaacggtctg
    19201 aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct tatgaccgct
    19261 tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg cggcgcattg
    19321 agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc aaaaatgctg
    19381 acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg acgaatattc
    19441 atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct cgtcgactga
    19501 aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg gaaatgaacg
    19561 gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc aacagtaacg
    19621 gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg gagattgttg
    19681 ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag gtgcgggtga
    19741 actgcgcggc ggcgaatctg aatattgcca acggcgtggc gcgcccgcag atttttgctt
    19801 tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct tcagaacagc
    19861 tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg cgttcgccgc
    19921 tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga ccgctgattg
    19981 cccgtggtgc tgttgctgcg gcactggcaa cgctggtaac accgacggcg gcgttactgg
    20041 cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg tcgcagatga
    20101 agaagtgatg ataattgccg gatgtctgac atccggcaat taaacagatt tagatttttg
    20161 gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaatcc
    20221 ttgtgaattc aatatattgt atggaaattc agtcctgata agcgtagcgc atcaggcttt
    20281 tcacacttat tacaacgact gatgtcgcgt ctcatgggtc agcagcaggg cgattaacgt
    20341 caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt tagtctgcaa
    20401 ccaggctgcg atatacggtg caacggaagc cccgagaatc gacgctacgt tgtaagagaa
    20461 tgatgctccg gtgtaacgca cttctgttgg gaacagctct ggtaacagcg cgcccatcgg
    20521 cccgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa ccagaatcgg
    20581 gttgccagaa ccgagcagcg ggttaaaggc gaacagcgcg aacaggatga tcagcgtggt
    20641 gataattacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccgg cgactggcac
    20701 catcacgcca aaaccaataa ctgccatcat cagcatccac aacacttcgt tacgcggcag
    20761 gccaagccca actggcgcgg cggcggtact gaaggtcata gagtagaccg tcatgatgta
    20821 aaacagcgta taggttgcca gcatgatgaa cgtacccagt acggttacgc gaacatgttt
    20881 ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga ctttctcaaa
    20941 caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca gtaccgccga
    21001 gaagataaat ggcacacgcc agccccagct cataaactgc tcgtcggtca gtagccagga
    21061 aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccca gctgtgggaa
    21121 ggagccatac agtgcacgtt tgcgcggtgg ggcgttttca gtcgccagca gcgccgcgcc
    21181 gccccattca ccgcctaagc ccagaccctg accaaatcga gccagcgcca gcagcagcgg
    21241 ggcgaaaata ccaatcgtgg catagcccgg cagcagacca atcaccacgg ttgaaatccc
    21301 catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc caaaatgacc
    21361 aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg cgagcgactg
    21421 tagcgttgct gccgttggat cgccctgcgg gaagaagata tgcggaaaca caataacggc
    21481 cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggcc gtgccaatga gagaggcgac
    21541 aaggacttta ttacgcgagt tgatcggcgt gtattcttgc tcgtggtcga gtgttgtggc
    21601 tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt acgcacagca
    21661 aaagtgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg caaaaagggg
    21721 ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt tataaatagt
    21781 ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg ctggggttat
    21841 gagttggaat gaaattgttt gctcccaaat agatggctga agaggctctg gggaagagtt
    21901 tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta tccggcctac
    21961 ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc atccggccta
    22021 ttttaaggct gcgttttccc tggcattcgc gggtcgtctt tatattcggc ggtggcaatc
    22081 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    22141 acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt gtaggtcatc
    22201 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    22261 ttgcgcggac ggtggcgcgg taaccgccag aagatccaga agaaaagcag atagttggcg
    22321 aagatggaaa tcgccaggcc aatcaaccgc cacgtcggtt tcagccactc aatgctgttc
    22381 aggtgcaggt cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    22441 gtcacaatca gcgcaatcaa caaaccaatc agcgaaataa aatcacgtag atattttacc
    22501 cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg ggcgcgaatc
    22561 gcttcacgca ggttacccat ccagttgatg ccggaataaa gcgccacagc caggccgaca
    22621 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtggcggct
    22681 agcgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    22741 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    22801 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    22861 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    22921 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    22981 atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    23041 tcttttgtcc ttctgttgtg gtcattctta aaaagtatag tcggtcagtc caggtactct
    23101 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    23161 tgcggataaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa cacttcaata
    23221 agctgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    23281 ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat gccgccagac
    23341 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    23401 tgcggtgtat aacgcaccat tgcgtgtgaa gtcagatcat cggggctttg cggataacca
    23461 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    23521 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    23581 agaatatcta ccggacggtt atggctactc agttccagct caattcccgg atgctgatag
    23641 agaaattccg acagacgcgg cagtaacagg tttttcgcga ttcctgacgg gatgtcgatg
    23701 cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag ttcgctgaga
    23761 ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt gagtttgacc
    23821 cgtcgcgtag tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    23881 cgcgaaacac ttccctttgg caaagcaaag aaatccgccg cgcgggaaaa actttccagc
    23941 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    24001 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc taacatctaa
    24061 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    24121 aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag tgattccctt
    24181 taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    24241 attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    24301 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    24361 gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg gccaccgcca
    24421 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    24481 cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    24541 ttcatggaat gaaaatcagt ttacagcagc gtattccagg agtgagtatt cagggggcca
    24601 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    24661 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcagtttc
    24721 ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta caggaagtta
    24781 ttcattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    24841 cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg acgacgccgg
    24901 aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt ttaaccatgt
    24961 tagcggcggg cgaatcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    25021 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    25081 caatgatgtt aaatatctcc tcctg
//
LOCUS       DAJTVK010000055        24870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000055 DAJTVK010000000
VERSION     DAJTVK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.055"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..796)
                     /locus_tag="OO372_002248"
     CDS             complement(<1..796)
                     /locus_tag="OO372_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6341726.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYL"
     gene            1363..1728
                     /locus_tag="OO372_002249"
     CDS             1363..1728
                     /locus_tag="OO372_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6341727.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1768..2424
                     /locus_tag="OO372_002250"
     CDS             1768..2424
                     /locus_tag="OO372_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR6341728.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            2491..3909
                     /gene="dcm"
                     /locus_tag="OO372_002251"
     CDS             2491..3909
                     /gene="dcm"
                     /locus_tag="OO372_002251"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6341729.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3890..4360
                     /gene="vsr"
                     /locus_tag="OO372_002252"
     CDS             3890..4360
                     /gene="vsr"
                     /locus_tag="OO372_002252"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6341730.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(4349..5270)
                     /gene="yedA"
                     /locus_tag="OO372_002253"
                     /pseudo
     CDS             complement(4349..5270)
                     /gene="yedA"
                     /locus_tag="OO372_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            5443..6360
                     /locus_tag="OO372_002254"
     CDS             5443..6360
                     /locus_tag="OO372_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6341731.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            6439..6621
                     /locus_tag="OO372_002255"
     CDS             6439..6621
                     /locus_tag="OO372_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6341732.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6792..8486
                     /gene="yedQ"
                     /locus_tag="OO372_002256"
     CDS             6792..8486
                     /gene="yedQ"
                     /locus_tag="OO372_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HCR6341733.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(8483..9298)
                     /locus_tag="OO372_002257"
     CDS             complement(8483..9298)
                     /locus_tag="OO372_002257"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCR6341734.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(9596..9823)
                     /gene="yodD"
                     /locus_tag="OO372_002258"
     CDS             complement(9596..9823)
                     /gene="yodD"
                     /locus_tag="OO372_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6341735.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            9986..10174
                     /gene="dsrB"
                     /locus_tag="OO372_002259"
     CDS             9986..10174
                     /gene="dsrB"
                     /locus_tag="OO372_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6341736.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(10218..10841)
                     /gene="rcsA"
                     /locus_tag="OO372_002260"
     CDS             complement(10218..10841)
                     /gene="rcsA"
                     /locus_tag="OO372_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR6341737.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(11126..11911)
                     /gene="fliR"
                     /locus_tag="OO372_002261"
     CDS             complement(11126..11911)
                     /gene="fliR"
                     /locus_tag="OO372_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6341738.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11920..12189)
                     /gene="fliQ"
                     /locus_tag="OO372_002262"
     CDS             complement(11920..12189)
                     /gene="fliQ"
                     /locus_tag="OO372_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6341739.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(12199..12936)
                     /gene="fliP"
                     /locus_tag="OO372_002263"
     CDS             complement(12199..12936)
                     /gene="fliP"
                     /locus_tag="OO372_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR6341740.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            complement(12936..13301)
                     /gene="fliO"
                     /locus_tag="OO372_002264"
     CDS             complement(12936..13301)
                     /gene="fliO"
                     /locus_tag="OO372_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6341741.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(13304..13717)
                     /gene="fliN"
                     /locus_tag="OO372_002265"
     CDS             complement(13304..13717)
                     /gene="fliN"
                     /locus_tag="OO372_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6341742.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            complement(13714..14718)
                     /gene="fliM"
                     /locus_tag="OO372_002266"
     CDS             complement(13714..14718)
                     /gene="fliM"
                     /locus_tag="OO372_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6341743.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14723..15187)
                     /gene="fliL"
                     /locus_tag="OO372_002267"
     CDS             complement(14723..15187)
                     /gene="fliL"
                     /locus_tag="OO372_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6341744.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            complement(15292..16419)
                     /gene="fliK"
                     /locus_tag="OO372_002268"
     CDS             complement(15292..16419)
                     /gene="fliK"
                     /locus_tag="OO372_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6341745.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(16416..16859)
                     /gene="fliJ"
                     /locus_tag="OO372_002269"
     CDS             complement(16416..16859)
                     /gene="fliJ"
                     /locus_tag="OO372_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR6341746.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16878..18251)
                     /gene="fliI"
                     /locus_tag="OO372_002270"
     CDS             complement(16878..18251)
                     /gene="fliI"
                     /locus_tag="OO372_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6341747.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            complement(18251..18937)
                     /gene="fliH"
                     /locus_tag="OO372_002271"
     CDS             complement(18251..18937)
                     /gene="fliH"
                     /locus_tag="OO372_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6341748.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            complement(18930..19925)
                     /gene="fliG"
                     /locus_tag="OO372_002272"
     CDS             complement(18930..19925)
                     /gene="fliG"
                     /locus_tag="OO372_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6341749.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            complement(19918..21576)
                     /gene="fliF"
                     /locus_tag="OO372_002273"
     CDS             complement(19918..21576)
                     /gene="fliF"
                     /locus_tag="OO372_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR6341750.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            21792..22106
                     /gene="fliE"
                     /locus_tag="OO372_002274"
     CDS             21792..22106
                     /gene="fliE"
                     /locus_tag="OO372_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6341751.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            22440..22772
                     /gene="emrE"
                     /locus_tag="OO372_002275"
     CDS             22440..22772
                     /gene="emrE"
                     /locus_tag="OO372_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6341752.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            22941..23492
                     /gene="ybcL"
                     /locus_tag="OO372_002276"
     CDS             22941..23492
                     /gene="ybcL"
                     /locus_tag="OO372_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6341753.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            23502..24299
                     /locus_tag="OO372_002277"
     CDS             23502..24299
                     /locus_tag="OO372_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6341754.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         6112 a         6150 c         6356 g         6252 t
ORIGIN      
        1 caagataaac attgttggcg tcgtattttg caccaaatgt ccatgcttca gctgtatcac
       61 caccagcaaa tttgctgtat tcattgtagt gatacccgcc aagagctact tgagaattgg
      121 tacggtcaga gtttgagtaa gcggcaccta agctcaaacc gaagtcaaag tcatatgaag
      181 tggaaagacc aaaaccatca ccgttctgga agcgaacgtc ttcgtgacca tttttagtac
      241 cttcttgatc ttctcctgca ttgctgcctt cgttgttacc ttgatactgc aatgcaaagt
      301 tcagaccttc caccagaccg aagaaatcac tattgcggta agtagctacc ccatttgcac
      361 ggcctgccat gaagttatca gtccaggtat aggagtcgcc gccgaataca ggaagcatat
      421 ctgtccaggc ttcaacgtcg taaatcacac catagttacg accatagtca aaggagccca
      481 tctcgccgaa cttcagacct gcaaaagcaa gtttgacata agtattatca gaaccttcag
      541 tgtcgctagc ctttgttttg gtcagtgctt gaccataacc agttaactga tctgtaattt
      601 gggtctcacc tttgaagcct aactcgatga tagtttcatc gccgtcatcg ccagggttat
      661 cggaaaaagt atgacgagca tcaacttttc cgtacagatc cagtttattg ccatttttat
      721 tataaatttc agccgcattt gctgcgccag caactaataa cgctgggacc agcattgcca
      781 gaacttttct tttcattatg tattcccttg tgattataat cttcatgaat atatcaataa
      841 gtgccgttat ccaaaaaaaa agcacatttg gatactattc tatgaagttc attttatttt
      901 aaagattgcg tgtaacaaat gtatttaaaa gatttcaaaa tgtttcttat ctatttgata
      961 taattcagaa ttatgaattc taaatataaa catccatgaa aattataaat gtatatcaat
     1021 atgttatgag ttgaatggaa agatagggag tatcaatgat tgttgcgcca aacggcaggc
     1081 gtgatcacgc gcatattaat gaaggtataa aaaaagccgg catcatgccg gcccaaaaaa
     1141 tataaacaag acgataaaag gctaataacg gggttatcat gacacagaaa tcaaaaaagg
     1201 tataattgta ttcagtgcta atatattgtt tgggtgaatt aataaaacta ttaccaagga
     1261 tataaataaa tctttattgc tgtttttcca gtaattattg ccgttgcgca aaatttatct
     1321 atttgttcaa aaaatgattg agtcttgact ggctcatcca atgtggaaaa atgtgacttt
     1381 tatcacataa tagtactaag tctgaatttt ccgggttatc tcaaaatgga atacggttcg
     1441 acaaagatgg aagagagact ctctcgcagc cctggcggca aattggcttt gtgggctttc
     1501 tatacatggt gtggctattt cgtctgggca atggcgcgtt acatctgggt gatgagccgg
     1561 atccctgatg ccccggtgtc gggatttgaa agcgatttag gctctaccgc gggaaagtgg
     1621 ttaggggcac tggtcggatt tttatttatg gctctggtcg gggcattgtt aggcagcatt
     1681 gcctggtaca cccgacctcg tcccgcacgt agcagacgct atgaataaaa actgctaagg
     1741 tggcgactct ttggcaacgg agcgcgtatg gacttacaac actggcaggc acagttcgaa
     1801 aactggttga aaaaccatca tcagcaccag gacgcggcac agaagctggc ggcagatgac
     1861 gatgtcgata tgctggtgat tttaaccgcc tgttattttc acgatattgt gagcctggcg
     1921 aaaaatcatc cgcaaaggca gcgttcttca atcctggcgg cagaagagac gcgtcgcctg
     1981 ctccgtgaag agttcgagca atttccggcg gagaaaatcg aggccgtttg tcatgccatt
     2041 gccgctcaca gtttcagcgc gcaaatcgcc cccttaacaa cggaagctaa aatcgtccag
     2101 gatgcggatc ggctggaagc cctgggggcg attggcctgg cgcgcgtgtt tgccgtttca
     2161 ggggcattgg gcgtggcgct gtttgatggt gaagatccgt tcgcacagca tcgcccgctt
     2221 gatgataaac gctacgcgct ggatcatttc cagactaagt tgctgaagct accgcaaacc
     2281 atgcaaaccg caagaggcaa gcagctggcg cagcacaacg cgcatttttt agtcgagttt
     2341 atggcgaagc tcagtgccga actggcgggg gagaatgaag gtgtcgatca caaggtgata
     2401 gatgcgtttt cacccgcggg ataagcgcgt ggccctaaat ggctgtaatt atgttaacct
     2461 gtcggccatc tcagatggcc ggttaaatct atgcaggaaa atatatcagt aaccgattca
     2521 tacagcaccg ggaatgccgc acaggcaatg ctggagaaac tgctgcaaat ttatgatgtt
     2581 aaaacgctgg tggcgcagct taatggtgtg ggtgagaatc actggagcgc ggcaatttta
     2641 aaacgtgcgc tggcgaatga ctcggcatgg caccgtttaa gtgagaaaga gttcgcccat
     2701 ctgcaaacgt tgttacccaa accatcggca catcatccgc attatgcgtt tcgctttatc
     2761 gatctatttg ccggaattgg cggcatccgt cgcggttttg aatcgattgg cggacaatgc
     2821 gtgtttacca gcgaatggaa caaacatgcg gtacgcactt ataaagccaa ccattattgc
     2881 gatccggcga cgcatcattt taatgaagat atccgcgaca tcaccctcag ccataaagaa
     2941 ggcgtgagtg atgaggcggc ggcggaacat attcgtcaac acattcctga acacgatgtt
     3001 ttactggccg gtttcccttg tcagccattt tcgctggctg gcgtatcgaa aaagaactcg
     3061 ctcgggcggg cgcacggttt tgcctgcgat acccagggca cgctgttttt tgatgtggta
     3121 cgcattatcg acgcgcgtcg tccggcgatg tttgtgctcg aaaacgtcaa aaacctgaaa
     3181 agtcacgacc agggtaaaac gttccgcatc atcatgcaga cgctggacga actgggctat
     3241 gacgtggctg atgcagaaga taacgggccg gacgatccga aaatcatcga tggtaaacat
     3301 tttctgccgc agcaccgtga acgcatcgtg ctggtgggtt ttcgtcgcga tcttaatctg
     3361 aaagccgatt ttactctgcg tgatatcagc gaatgtttcc ctgcacagcg agtgacgctg
     3421 gcgcagctgt tggacccgat ggtcgaggcg aaatatatcc tgacgccggt gctgtggaag
     3481 tacctctatc gatatgcgaa aaaacatcag gcgcgcggta acggcttcgg ttatggaatg
     3541 gtttatccga acaatccgca aagcgtcacg cgtacgctgt ctgcgcgtta ttacaaagat
     3601 ggcgcggaaa ttttaatcga tcgcggctgg gatatggcca cgggtgagaa agattttgac
     3661 gatccgctga atcagcaaca tcgtccacgt cggttaacgc ctcgggaatg cgcgcgctta
     3721 atgggttttg aagcgccggg agaagcgaaa ttccgcattc cggtttcgga cactcaggcc
     3781 tatcgccagt tcggtaactc ggtggtcgtg ccggtctttg ccgcggtggc aaaactgctt
     3841 gagccaaaaa tcaaacaggc ggtggcgttg cgtcagcaag aggcacaaca tggccgacgt
     3901 tcacgataag gccactcgca gcaaaaatat gcgcgcgatt gccacgcgtg atacggcgat
     3961 tgagaagcgc ctcgccagtc tgttaaccgg gcagggcctg gcgtttcgcg ttcaggacgc
     4021 cagtctgccc ggaagcccgg attttgtcgt tgatgaatat cgctgcgtga tatttaccca
     4081 tggctgcttc tggcatcatc atcactgcta tctgtttaaa gtgcctgcga ctcgaaccga
     4141 gttctggctg gagaagatag gtaaaaatgt tgaacgcgat cgccgcgata tcagtcgttt
     4201 gcaggaactc ggctggcgcg tactgattgt ctgggagtgt gcattacgtg ggcgcgagaa
     4261 gctgacggat gaagcgctta ccgagcgtct ggaagagtgg atctgcggcg aaggtgccag
     4321 cgcgcagatc gacacgcagg ggattcattt actcgcttga tgcgtcctga ataactggcg
     4381 caactacggg ttttgccggg aagagatatt ttcccaacgt gaccagtacc accgcgaaga
     4441 caattacgcc gagcgccagc cattcaatct tcgacagcgt ctctccgccc agtcccgtac
     4501 ccagcaagac cgcgaccacc gggttaacgt aggcgtagct ggtggcgaga gccggactga
     4561 cattacggat taaatacata taagcgttga tggcgataat cgaaccaaac agcgccagat
     4621 agccgaccgc aaggaagcct gaaagggaag ggagcgccgt cagtttttca cctgcaatca
     4681 tcgacgcgat cattaacacc acgcctgccg ccagcatctc aatcgcaccc gccatcatcc
     4741 ctacaggtaa ggtaatgcgc gagccataaa ccgagccaaa cgcccagcta atcgagccga
     4801 ttaaaatcag aatcgcgccc cacggattgc cgcttaaatt tccaccgcta ttgagcatga
     4861 tgattccggc aagcccaatg gcgataccca cccattccag tttgcgcgtt ttaatgccaa
     4921 acaggcggct gaagcaccag ggtaaagagg ggcacggttg caaccacaac ggcggcgatg
     4981 ccggaaggaa cattttgatg ctcggcaacc gtcaccatgc cattaccgac agccagcaat
     5041 aacaggccaa tcagcgcggc attgagcagc ggacgtagcg gggggagctt gtgtccgcgc
     5101 agtagcaaaa atgccatcaa taaaataccg gctgccagga atcgaacgcc tgccatcatt
     5161 aacggaggcc agctttccac gccaatccga atgacaaaat aggttgagcc ccaaatgata
     5221 tacaacgcaa acagcgcgcc aaaaagcggt aacaactggc ggaaacgcat aatccctcac
     5281 ggtggaaata aaaaggtggt tcatagtaaa cgtgaaaatc attctgctgg cgagagatat
     5341 aattgcactt gattgtaaaa aaaatgttga cctgtgaagc aagtatcaga gctgccgttt
     5401 ttgcttcata cttacaccct tcaacaataa aaatgagagg gaatgctttt ggccgggagt
     5461 agtttactga cgttgctcga tgatatcgcc acactactgg acgatatctc cgtgatgggc
     5521 aaactggcgg cgaagaaaac cgccggtgta ttaggggatg acttatcgct caatgcgcaa
     5581 caagtttcag gcgtgcgggc caaccgggaa cttcccgtgg tctggggcgt ggcgaaagga
     5641 tcgctgatta ataaagtgat tctggtgccg ctggcgctga tcatcagtgc gtttatcccg
     5701 tgggcgatta cgcctctgtt gatgattggt ggcgcgtttc tctgctttga aggagtagag
     5761 aaagtgctgc atatgctgga ggcgcgtaaa cataaagaag atccggcgca gagccagcag
     5821 cgtctggaga agctggcggc gcaggatccg ctgaagtttg aaaaggacaa aataaaaggg
     5881 gcgattcgta ccgattttat attgtctgcc gaaatcgtcg ccatcacgct ggggattgtg
     5941 gccgaagcgc cgttgcttaa tcaggtgctg gtgctttcag gcatcgcgct ggtagtgacc
     6001 gtgggcgtct acggtctggt aggggttatc gttaagattg atgacctggg gtattggctg
     6061 gcggaaaaat ccagcgcgct gatgcaggca ttaggtaaag ggttattgat cattgcgccc
     6121 tggttgatga aggcgttatc gattgtcggc acgctggcga tgttcctcgt cggcggcggt
     6181 attgtggtac atggtattgc gccgctgcat cacgccattg aacatttcgc cgggcagcaa
     6241 agtgcagtgg tggcgatgat attaccgact gttttaaatc tgattcttgg atttatcatc
     6301 ggcggcatcg tggtgctggg agtgaaagcc gtagcgaaaa tgcgcggtca ggcacattaa
     6361 tactcgtaca ggctattatg caaaattcgc catcttcaac taaggtgaac aagtttcact
     6421 cgaaaaaagg aggcgagtat gagctttatg gttagtgagg aagttacggt taaagagggc
     6481 ggcccgcgga tgattgtcac tggatactcc agcggtatgg ttgagtgtcg ttggtatgac
     6541 ggttacgggg tcaagcggga agcttttcat gaaaccgagc ttgttccggg ggaggggagt
     6601 cgttctgcgg aagaagtttg aaagggtatc attaacacca ggccggatca cttccggcct
     6661 ggtgtgccaa tgcctgcaat gaatgatagt cgcgtcttat catgccacca ttataaaatt
     6721 atcgctgttt tgcggtacgc tatcgccagg ttatgccaga atcataaaaa agcaggttgg
     6781 gagtcgtcag ggtgcagcac gagacaaaaa tggaaaacca gagctggttg aaaaaactcg
     6841 cacgccgcct ggggcctggt catgtcgtta atctctgctt tatcgtggta ttgctttttt
     6901 ccaccttgct gacctggcgt gaagtggtag tgctggaaga tgcctatatc tccagccagc
     6961 gtaatcatct ggaaaacgta gccaacgcgc tcgataagca tttgcagtat aacgtcgaca
     7021 aactgatctt tttgcgtaat ggcatgcgcg aagctctcgt agcgccactg gatttcactt
     7081 cactgcgtaa tgctgtaacc gagttcgaac agcatcgcga cgagcacgcc tggcaaattg
     7141 aactcaaccg acgacgcacc ctgtcagtca atggcgtatc ggatgcatta gtcagcgagg
     7201 ggaatctcct gtctcgcgaa aatgaaagcc tcgacaatga aattaccgct gcactggaag
     7261 ttggttactt gctgcgactg gcgcacaaca cctcgtcgat ggttgaacag gcgatgtatg
     7321 tctcgcgtgc cggattttac gtttcgacgc agccgacctt gtttacgcgc aatgtaccaa
     7381 cgcgttatta cggctatgtc acccaaccct ggtttatcgg ccattcgcaa cgagaaaatc
     7441 gtcaccgcgc ggtacgctgg tttacttcgc aaccggagca cgccagcaat actgaaccgc
     7501 aggttaccgt cagtgttccg gtagacagta ataactactg gtatggcgtg ctggggatga
     7561 gtattcccgt gcgtaccatg cagcaatttt taagaaacgc catcgataaa aacctcgatg
     7621 gtgagtatca gctctatgac agtaagctga gatttttgac ctcttccaat cctgaccatc
     7681 caacagggaa tatttttgat cctcgtgaac tggccttgct ggcgcaggcg atggaacatg
     7741 acacgcgggg cggcattcgt atggacagtc gctatgttag ctgggaacgt ctggaccatt
     7801 tcgacggtgt gctggtgcgc gtccatacgc taagcgaagg cgtgcgcggc gatttcggca
     7861 gtatcagcat tgcattaacc ctgctgtggg cgctctttac caccatgtta ctcatctcct
     7921 ggtatgtgat tcgccggatg gttagcaaca tgtatgttct gcaaagctcg ttgcagtggc
     7981 aggcgtggca cgacacctta acccgtttat ataatcgtgg cgcactgttt gaaaaagccc
     8041 gtccgctcgc gaaattgtgt cagacgcacc aacatccttt ttctgtcatt caggtcgacc
     8101 ttgaccattt taaagcgatt aatgaccgct ttggtcatca ggcgggcgac cgtgttcttt
     8161 ctcatgctgc cggattaatt agcagttcct tgcgtgcgca ggacgttgcc gggcgggtcg
     8221 gtggtgagga gttttgtgtg attctgccag gcgcgagtct gacggaggct gcggaagtcg
     8281 cagaacgtat tcgcctgaag ttaaatgaaa aagagatgtt gattgctaag agtacgacga
     8341 tacgcatcag tgcctcgttg ggggtaagta gcagcgagga aaccggtgat tatgattttg
     8401 aacaactcca gtcactggct gaccgtcggc tttatctcgc taaacaggcc gggcgtaatc
     8461 gggtatgcgc gagcgataac gcttaacgag cggagaaaaa atggtccagc ccttcacgcc
     8521 agccttccgg tccttcacgc tgcgttcgcc agacgcgggt cggatcctca tcatgcagat
     8581 gcaccccttc acggtttagc cctttcacaa tcaccgcgta atccattacc tccagtaagg
     8641 gcgcatcgtt tggcccatcg cccaggccaa gtgtggttgg gcgtttgcct gataattgtt
     8701 gataggtcgc gataatccag ttggcggcct gatcttttcc ggcagaggca tccaggacgt
     8761 gccagaagcg cgcaccctgc ataaactgta agcccagttc gttcagacga gcggtaaatt
     8821 gtgccatacg ctcgtcactg tcgcgccaga ttagcgttac cgacgcctca tgcagctgcg
     8881 tcagcgccgc ctggctacgg cttaatcccg tccattcggc gatggttgca tcgtcgacat
     8941 catcgaaagt cgtgaattta aaatgttctt tctcgcgtag cgtatttaaa acctggctga
     9001 tttcgccatg gctaatacct gagatgatgc gtggaaaacc gtctatatcc tgccattgtt
     9061 cagcaagctg gatcactgcg ccattttcgg caatcagtgg taacccttgt agccccaacg
     9121 ttttttgcaa gtacagcatt tccgctgaag ttttactgct acagagaatg acgggaacat
     9181 tcgcttcgcg taaacggctg agccagaggg cagtcggttg ccagtcataa ctatgactgt
     9241 ccagcagggt gccatcaaga tcgctaaaaa ccagtagtgg ttgttgaatt gaaaacatgg
     9301 tcgtgcagtt ctccccttac caggatgatt ctcaaaacag cacaaataat caaagatgat
     9361 tttttcggga gcgatcccgt tgacgccaga gccaccaagg ggtgacgcac gtcacatttc
     9421 tattcataag tagcgttaat tattcatatc gcgaatattt tcttgtcagc gaaaaaaatt
     9481 gcggataagg tgatgaacac atcagatttc ctggtgtaac gaatttttta agtgcttctt
     9541 gcttaagcaa gtttcatccc gaccccctca gggtcgggat ttttttattg tgcattcaac
     9601 gattcacttc agagacgctg aagtcatgaa tatccagttc gaaggcatcc gccaattcac
     9661 gccatgtatg atattcacgt ccatcgacgc taacgtgttc agaatcgttc tggctgcgat
     9721 gaacttcgct ttcgctaatt tcattgatcg ccgccagcag ggcatcgaca tcgacgctga
     9781 cctcacgttt tgcggtatcg ctgtactctt ttgcggtttt catcatcttt cctcgcaacc
     9841 gttactctgt tgttgccgcc agtcgttaag tccgcctggc atctcttaag tatagaccgt
     9901 cgagaaaatc agcactcgcg cgcgcctgga gctgccagaa aaacgaaatt gttctacact
     9961 ggcacaaagc cacaggagga aaacgatgaa ggtgaatgat cgggtaacag tcaaaacgga
    10021 tggcggtccg cgtcgtcctg gcgtggtact ggcagttgag gagtttagtg aaggcacaat
    10081 gtacctggtt tcgctggaag actacccgct cggcatctgg ttctttaatg aagcagggca
    10141 tcaggacggt atctttgtgg agaaagcaga gtaatcttat tcagcctgac tggtgggaaa
    10201 ccaccagtca gaatgtgtta gcgcatgttg acaaaaatac cattagtcac attatccgtc
    10261 agtcggacga catggtagat aacctgttta ttatgtgttt tgatcttacg tttaatatta
    10321 cctttatgcg atgaaacggt cttggctttg atattcattt ggtcagagat ttgaatggtt
    10381 ccctgacctg ccatccacat tcgcaacata ctcgattcgg ttcggctcaa tgataacgtc
    10441 ggcatattta aaaaagaggt tatcgttgtc tcttttttca gaatatcgcc aaggatatcg
    10501 tcgagagatt ccggtttaat cgatttagaa cttatcaata atttttttct gaccaataga
    10561 tattcatcaa aatgaacatt ggcaattgcc ataaaaacga taaataacgt attgggatgt
    10621 tgattaatga tgtgcttgat gtgctgactg ttagaagcat catggatgaa acagtcctca
    10681 ttaataaaca ccactgaagg gcgctgtgaa tcacaagcta tggcaaggtc atcaacggtt
    10741 tcaatgtcgt tgatttctct ttttttaacc cctctactca acagataccc ggttaaacct
    10801 agtcgggtgt aactacataa atccataata atcgttgaca tggcataccc tcactcaatg
    10861 cgtaacgata attcccctta cctgaatatt tcatcatgac taaacggaac aacatgggtc
    10921 acctaatgcg ccactctcgc gatttttcag gcggacttac tatcccgtaa agtgttgtat
    10981 aatttgcctg gaattgtctt aaagtaaatg ttgcgatatg tgagtgagct taaaacaaat
    11041 atttcgccgc aggagtatcc tggaagatgt tcgtaggtat tatttttcgg ataatcctta
    11101 ggataacatg ataaacgtta cggaattata ttaatggcaa ttcactaata atatcagcca
    11161 gcaaattaaa catctcactg aataaatgtt cgcaaaaagg tgcaattaac ggcattaatg
    11221 ccgccattaa agagataccg acagtcagag ttaatggaaa tccaataaca aaaatggata
    11281 attgcggggc catacgattc agtaaaccta atgccagatt cagggtcagc agcagagtaa
    11341 tgagcggtaa cgccagcatc agcccgttaa ggaaaatcaa actccccgct ttggtgagtg
    11401 ccagaaacgc attgctgttc aacggttcgc caccaattgg cagggtgtga aaggtatcga
    11461 ccagcagtga aatcaaccat aaatgaccgt taaatgtcag gaacagcagt aacgccagca
    11521 tatccatgat acgcgctaaa acgggcatat tgagatggct ggccggatcg acaaacgtcg
    11581 caaatgacag ccccatttgc agaccgataa tttcgccagc ggttcgcaca gcggcaaagg
    11641 caaattgcat ggtaaaacca agcgcaatgc cgatcaggat ctgctgcacg gccagccaca
    11701 gagcaaagaa cgaaaaaaca ggaacatcgt tggcaggtaa tgatggggca atgacgaacg
    11761 tgatcatcat tgccagcccc agttttaccc gtttcggcac gctgcgttcg ctcagaattg
    11821 gtgcggtaga aatgagcgcc agcacgcgca gtaacggcca gaagtacagg cttaaccagg
    11881 aaagccattg atcgcttgtc acctgcatca ttgtacggcc tacccgatga tatacggcag
    11941 gttagtgaac aaggtgcgga cgtaatccag caacagattg agcatccacg gtccggcaat
    12001 aataatggca ataaatacgg cgatgatttt tggaataaac gacagcgtca tttcgttgat
    12061 ctgcgtggcg gcctgcaaaa tactgatgat aaggcccgtg accagcgcca ccagcaacag
    12121 cggggcagcc agtgccagcg cgactttcat cgcttcagtc cccatcatca tgaccgattc
    12181 aggtgtcatt ttgcctctct agctgtaaaa gctctgcgcc agcgaaccga ccagcaattg
    12241 ccagccatcc accagtacaa acagcatcag tttaaagggc agagcaatgg tggcgggggg
    12301 gaccatcatc atccccaatg ccatcaacac gctggcaatc accaggtcga taatcaaaaa
    12361 aggaatgaaa atcgtgaagc ctatctgaaa tgcggttttc aattcgctgg tcacgtaggc
    12421 cgggagcaaa atgcgcatcg gcacggcttc aggtccctgc aacgggccgg tattcgctag
    12481 tctggcaaac aaccctaaat ctgcctcacg ggtctgacgc agcataaact cacgtagcgg
    12541 ctgcgcccct ttttccagcg cctcctgcat tgatattttc tcttcgctga atggctggta
    12601 cgcatcgaca taaattttgt cgatcaccga tgacataata aaaaaggtca aaaatagtgc
    12661 cagccccagc aatacctggt taggtggcgc ggagggcgtt cccagcgcgt tacgcaataa
    12721 accaaaaaca atgatgatgc gggtgaaact ggtcatcatc agtaaaattg ccggaataaa
    12781 cgtcaacgag gtgatgaaca ccagcgtctg caccgggagc gaccagcttt gtccaccgcc
    12841 aggcagcggc tggctggtga tacccggcag ttgcgcgaag gcgaggggcg taaccagcca
    12901 gagaaggaca ggtgcgacag acaacaaacg acgcatcagg atctcccact acgcttaagc
    12961 aaatttttca tgaccgactg aaaatcggtc tgcggtatct cttctgttgg tgctgaaggg
    13021 ggaagtttat gcagcagatt gatttgccct gcggtaacgc cgagcaccag ccgtgcatct
    13081 tccacatcga ccaccacaac ccgttcacgc gcgcccagcg aagcactggc gctaattttc
    13141 agaccgttaa cgccagtgcg tttcggggca aatcccaacc gttttaccag ccaggcagca
    13201 gcgaggatca gggcaataat ggcgatgagt gcgccgctga cctgcagcag tggcgcagca
    13261 gaaaccggcg tggaagattg cacagtagcg tggttattca tcactaacgg ctcaggcgac
    13321 gcattcgctc agacggagta atgatatcgg tgatccgcac gccatattta tcggcaacga
    13381 ccaccacttc gccctgggag attaaataac cgttgatcag aatatccagt ggttcgcccg
    13441 ccagaccgtc cagcgccacg acggaccctt gcgtcagacg caacagctct ttgatggtca
    13501 tccgcgtacg gcccagctcg acggtcagct tgaccggaat atccattatc aggtcgatat
    13561 cctgcaacgt tccgctgaca tcaccaccgc caaattgctg gaacaccgcg tcggcagcgc
    13621 ttttgctgct ggttgatttt tgttcgctca acgcttcagc ccacagatcg tccattgcgc
    13681 cgttgttgtc atcggccgga ttattcatgt cactcatttg ggctgttcct cgttcagaga
    13741 atttaaaatc gggttaatca aatgttctat ccgtaacgca tactgaccgt tgagagtgcc
    13801 gtactgactg gtcagcaccg ggacgccgtc aacatgggcg atgatgccat cgggtttttc
    13861 tatcggcagg acgtcgccgg gtttcagttt taaaatctgc gacaggcgta gcgagatatc
    13921 ggcaaagttg gcgaccagct ccagctgtga atgctgcacc tggcgcacca ggttatcgcg
    13981 ccagttctga tcttcattac gcgagttttc cagcggcggg ttaaccaaca attcccgtag
    14041 cggctcgatc atgctgaatg gcagacagat attaaattcg ccggtcaggt tgccaatctc
    14101 cacatggaac ggcgtgttaa ccacaatgtc gttcggcgag gtggtgatat tggtaaattt
    14161 cacctgcatt tccgaacgca cgtactcaac ttccagcgga ttaatcgcct tccaggcgtc
    14221 gctatagcct tcaagcgcca gctttaacat gcggttgatg acgcgctgtt cggtatgggt
    14281 aaactcgcga ccttccactt tggtcgggaa gcgtccattg ccgccaaaca ggttatccac
    14341 ggcgataaac accagactcg gtgaaaacac caccagccct gtgccgcgca gcggtttcag
    14401 atggataagg ttcaggttgg tcggcaccgg caggttgcgg gcaaattcat ggtacggctg
    14461 aatgcggatg gccccgacgg ttatatccgg gctacgacgc agcaggttga acagccccat
    14521 acgaaaatgg cgggcaaagc gctcattaat gatttccagc gcctgcaaac gttcgcgcac
    14581 aacccgtcgt tgggtattcg gatcgtacgg acgaatgtca ctttcgccgc taacactggc
    14641 tgtcggttcg tctttgactt cgctgtcacc attcaacagc gcatcaattt cagcttgaga
    14701 aagaatacta tcgcccatgt cgttatcgca gaataaaagc ggtatacatc acgtcggtga
    14761 catcctgttt cggttgcccg gcaacaagcg gggtggaaag cgtggtttta atctcggcaa
    14821 tcaggttttt cttgccttct tctgtcgcca gtacggcagc atcctgacgc gaaaacagta
    14881 acagcaaacg gctacggact tccggcaaat actcactcag ccgcgagcgg gtagcttcat
    14941 ctttgagtcg cagggttata ccgatataaa gcacgcgatc cgcatcgccc aaattgaccg
    15001 taaaggtatc cagcgcatag aagaccggtg agggcacgac gcgatgctgc gctttgtctt
    15061 cagcggcaac ctgatgcgaa tgccagtagc tgtaacctgc gctggcacag gccgcgaggg
    15121 taatgaatac cagaatcggg agccaaagcg atcgcttgct tttcttgctt atcgcgtaat
    15181 cagtcatgtg ttgcggtctt cctgtgtcgc tactgcttat cggctaatta tcctgcgtct
    15241 tgagcgcaac aaagcgggga aaagacgcgg attacggtgc tacctctgac gttaggcgaa
    15301 aatatcaacg ccgctgttgc ctgttacgcg cccttgtaaa gagacgggaa ccggaagcgt
    15361 atcgtcttct tcccccgcca gaggttcatg gtttactgtg cgttggcttt gctgttgttg
    15421 ggaagcggcc tgctgctgac cactaaagct ttcgccactg atgttgcttt gccctaactg
    15481 aatgccactc tcggccagct gcgtacgcag taccggcagc gctgcttcca gggcggcgcg
    15541 tacgtgctga tgcggcgaaa ccatctggat ttgcgcctgg ttatcatcca ctttgaggga
    15601 gatttgcact tcacctaaat cctgcgggtg cagacgcaac tctgcacttt gttgtccctg
    15661 gcgggtgaac agcgaaatat gctggcttaa tgattgttgc cattcgtgag aacccagcgg
    15721 tgcactcaag acaggcgcgg cgacggtgag cagtggctgt gtctggtgtg gagtgattag
    15781 cgggctggcg gcagcggtca ccggtgaagg tgtgctgatg acttccgctt tactctgggc
    15841 ttctgctacc agcggtgtta atggctgagc tggcgtgccg ggggcatcat caggctgtgc
    15901 tgttgtgagt tgcgcagaag tcagttttgt gaagagcgtt ggtttctctg ccggtaacac
    15961 ggttgacggc gcatcagtca ctttgggcgt attgtcaaaa cccggcaaca tcgcaaaaag
    16021 ggcgctcagg cttgcggtga ggtcttcatt cagatcatcc gctttttcgt cttttgtcgt
    16081 atttttgtca gccaccgcag ccaacgtcat cgtatgagcg gtggttaacg gtgtcgatgt
    16141 ggattgttcg acgttgatga caggcagtgt ttcatccaca gggatcagta aatccgcttg
    16201 ttgcgcgtcg gaaacaatat cgctgatcag cagctcgcct tttgtcgtgg gcttatctgt
    16261 tgccaccagc acctgggggg ccgctttgtc ggtagttgtc tcgcttgcta atgcttcgct
    16321 caacaacgcg agaaaatctt gtgcagcatc gctggctttg ccgccaggca atgtggtggt
    16381 gtcaacgtcg gcggtaatca agggcgctaa gcgaatcatt caggtttcct catggcggcg
    16441 cgctgggcga actcatccat ctttttctga tcgaggcggt tttctgcaag cagtgccgcc
    16501 gtggattgcc gttcctgcag tgtctgccag gcctgcaaac gttgtttctt ttctcgccag
    16561 ctgttcaggg caatgtcaac tttctgcgtc cactgattaa gttgctggcg atgctgagta
    16621 atggcttttt ccagcgtctg gataaactgc tgatagttga tccagcggtt gctggtcatc
    16681 ccggcactca tatcgctgtt gaggttattg cgatattcat tctgataatc gatcagcatt
    16741 ttgagctgtt cttccgcctg ctgacacccg cgacgcattt cacccagcag gcgcgcggca
    16801 tcctctacct ctttttctgc cagatctttc agggtcgcca gcgcaccatg ttctgccatc
    16861 taccgttatc tcctgggtta tgacactgtc gggaaaatac gctccagccc ctggagagac
    16921 gcttcccagt ccgcacgttc aaaaatgcct tgttgtaaat agccctccag ctgcggccac
    16981 agggcgatgg ctttatcgag catcggatcg ctgcctttgg catacgcgcc gacgctaacc
    17041 agatcgcggt tacgctgaaa actcgacaac agctgtttga aggtgcgcac tcgcgcgtaa
    17101 tgttgctcac tgatcaacgc cgtcattgcg cggctgatcg acgcttcaat atcgatagcc
    17161 ggatagtgcc cggcttccgc cagtcggcga gacagcacaa tgtgaccgtc gaggatcgcc
    17221 cgtgcggagt ctgcaatggg gtcctgctgg tcatcgcctt cagtaagcac ggtataaaac
    17281 gcggtaatcg agccgccgcc gctaatgcca tttccggcac gctcgaccag tgccggtaat
    17341 ttggcaaaca ccgacggtgg ataacctttg gtggcgggtg gttcgccaat cgccagcgca
    17401 atttcacgct gggccatcgc gtagcgggtg agggagtcca taatcagcaa cacatgctga
    17461 ccacgatcgc gaaaatcttc ggcaatgcgc gtggcatagg cggcaccttg cattcgcaga
    17521 agcggagaga catccgccgg agcggcaatc accacggagc gtgcacgccc ttcagcaccg
    17581 aggatattct caataaaatc ttttacttcg cgcccacgtt caccaatcaa acccacgaca
    17641 atgacatcgg cgcgggtgta acgtgccatc atccccagca gcacactttt accaacgccg
    17701 gacccggcaa acagccccat acgctgccca cgcccaacgg taagcagggc attgattggg
    17761 cgcacgccgg tgtccagcac atgttcaatc ggtgtacgtt gcaacgggtt aaatggcggg
    17821 gtaatcagcg caccggtttc cgtcgtatcg ggggagggca ggccatcgag cggcttaccg
    17881 ctgccgtcca gaacgcgacc taataacgcc ggaccgagcg gcaactgctt gccgctttgc
    17941 agtccttcag ccgaaatgtt tttggcataa acccgcgcgc cgggcaggac accttccact
    18001 tcctccagcg gcattaaaaa cagccgttga ccgttaaagc caacgacttc gctttctact
    18061 tcgtgcgttt cgctgccgtt ctggcgttca atgacacagg ttgcgccgag cggcaattgt
    18121 aatccggtgg cttccagcac cagcccggta gcgcgggtta atcgcccata gcgacgtacc
    18181 gcaggcaact gcgccatttt ggcttcaaag ttatccagcg tggttagcca gcgagtcagg
    18241 cgcgtggtca ttacaccact cctggtgctg ccagacggca gagttcttgc cagcgagtgg
    18301 cgacactggc gtcgagatcg ccttcatcgg cggagacttt acagccgcca ggatggagtg
    18361 tgggatcgcc ccgcaagcgc cagccatgca aacttaaggt tgcgccgagc atatcatcca
    18421 cacgttgcag atcatccggg tgcacgcgca gctgtggttt accgctgaat aacggttctt
    18481 gctgcaacaa ctgttggatc tgtttgatca gtgccgagtt atccactgtt ggcgtctgac
    18541 cgatcacctg acgtgccgcc tccagcgcca tctgcatcag gcgcgaagca atcacactat
    18601 caagcgcatc aagggtagtt tgaaattcgc tgaccagttg ctgcatccgg gcatgaattg
    18661 gcgcttgttg agacttcgcc tctgccagac cttgctccag cccctgggcc agtccttcct
    18721 gatagccctg ctcatgacct tgctggcgac cttcggcaat ccccgcctga taaccttgct
    18781 catgggcctg catttgcagt tgcgccagtt gctgctcaag gctaggttcg gcctcttcaa
    18841 tgatggtttc ttccggctcg accatgggca caaactctgc ctgtggtggc gcgagatcgt
    18901 ccggcgtcca ggttttccac ggcagattat cagacatagg tatcctcgcc gctgccaatt
    18961 accatctcgc cagtttcggc aaggcggcgc acaatcagca gaatcgcttt ctgttcgttt
    19021 tccacctgcg acagacgcac cggaccacgg ttggcgagat cgtcgcgcag aatatcggcg
    19081 gcacgctgcg acatattgcg caggaatttc tcgcgcagtg gctgctcggc ccctttcagc
    19141 gcgatcaaca gcgattcgga atccacttcc tgcaacagac gctgaatgct gcgatcgtcg
    19201 acatccacca gattttcgaa caggaacatc tcgtcgataa ttttttgcgc cagctcgccg
    19261 tcgaattcgc gcacggcggt aataacggct tcttcctgct gagttttcat caggttgata
    19321 atttcggctg ccgttctcac gccgcccatt ttgctgcgct tgagattctg accgtcgagc
    19381 aagccattca gtacttcggt cagttccgcc agcgcggctg gctgcacgcc gccaaaggtg
    19441 gcgatacgca acatcacgtc gtggcgcaga cgttcatcga acaacgccag aatatcggcg
    19501 gcctgcgcgc gcttcagatg caccagaatg gtggcgataa tttgcggatg ctcatcgcga
    19561 atcagatcgg cggcactctg cggctccata aagttgagcg tttcaatacc gctggcggta
    19621 tcgcgagttt cgagaatatc ttccagcagg ctggcggcac gttcctcacc cagagctttg
    19681 accaataccg aacgcagata atcgttggcg ttgatattca gtgcggcaaa ctgttcagct
    19741 tcttgctcaa actccgccag cacataggtt agctgcttgt tggagatctg cgtgacgttc
    19801 gccattgcag cgctcagggt ttgcacttcg cgctgggaga ggtgcttgaa cacctctgcc
    19861 gcccggtctt cgccaatggt catcagcagg atgacgcttt tatcggtgcc tgtcaggtta
    19921 ctcatgatcg ttatttatcc actggcgaat gaccagcgcc accacgcgcg gatcgttatc
    19981 agacatttca cggatacgct ggctcatgac ttctgccccc agacgttggt tagcgcgtcg
    20041 ttgttggagt tgttcgtctt tgctcaggcg gacttccacc gcatcttcca cttcctcacg
    20101 ggcctgcgcc tgttgctgta cagctttcat cgcctcggcg cgacgtgtta actgcgggcg
    20161 taccgctttc cgccacagca gccacgccac cagcagtacc agcaaccagc gaccggcagc
    20221 cagcaactga tcgataaacg cttgctgttg ccagaacggc agttctccgc cgctttcgtc
    20281 actgctattg aacggcgagt taacgacatt gagcgagtca ccgcgttttt cagaaaagcc
    20341 catcgcttcg cgggtcagat cttcaatttg cttcatctgt tcgttgctga gaggcaatgg
    20401 tttaccgtct ggcaaggttt tgtaattcac cactaccgcg actgacagac gttgcacatc
    20461 gcccacgttc attttggtat gacgaatggt gcgatcgact tcgtagttac tggtttcatt
    20521 ccgctgtgtg ctacgcggcc cgctattgct ggtggtgctc gcctgctgct ggcggttatt
    20581 ttgatttgcc ggaggcgtgc tgattggcgc gttattcgca ggtgccggtt gattcgacaa
    20641 cgcccccggt acgccgcccg gataaccgga accgctttgc tcgctctcat taagctggcg
    20701 tgaacgaagc gccgcatgag attcatcacc gttagggcga tactgttctt ccgtttgttc
    20761 tttactggcg aagtccagtt gcgccgtgac ctgggcgtga atattaccgt taccaacaat
    20821 aggcgacagg attgcttcaa tacgccgctg aatacggcct tcgacatcgc tggcatattt
    20881 caactgagcg tcattaagat cgcgcccgct ggtattggac tgggttaaca gatgtccgcc
    20941 ctgatccacc agcgtgacgt ttcccggcgg cagaccagca acggcgctgg aaaccaggtg
    21001 cacaatggcg ctaatttgcc cttcatcgag tgcgcggccg ggtaacagat ttaccgtcac
    21061 cgatgcagaa ggggattttt gttcacggac gaataaagac ggtttcggca ttgccagatg
    21121 tacgcgcgcc ccttttaccg ggccgatagt ttcgatggta cgagaaagct cgccttccag
    21181 cgcccgctga taattcacct gttcgctgaa ctggctgata ccaaactttt cctgatcaag
    21241 cagttcgaaa ccgaccgcgc cgcctttggg caaaccttgt tgtgccaggc gcagacgcag
    21301 ttcgtgaact ttatctgccg gaacttcaat agcgccgctg gcttcgctga agcagtaagg
    21361 aatattcatt tgcgtcagtt ggctgacaat tgcgccacca tcctgatcgg aaagattgct
    21421 gaataatgtg cggtagtcgg gggctttcgc ccacaggatc agtgcgacca tgaccgccac
    21481 tgccgcggaa ccggcaacaa tcaatggaat tttcggattc gcacgcaggc gattaagcca
    21541 ctcaagagat tttgtctggg ctgcagtcgc attcatcgcg cacctcgtgg ctgattatgg
    21601 acggcgttat tggcaaagtg gaacaagact cactcccggg tcagcaaact cgaaaaattg
    21661 acgctgccat tatttgtgct ttcgtttttt tctatccgcc aataaacccg ttttttttgt
    21721 tgctatttag cgcctttgtc ttattgactt actggtaagc tttgctacca gaaattatcc
    21781 gggagatgaa aatgtcagcg atacagggga ttgaaggggt tatcagccag ttacaggcta
    21841 cggcgatgag tgcgcgtgcg caggaatcac tgccacaacc gaccattagt tttgccgggc
    21901 agctgcacgc cgcgctcgat cgcataagcg atacacaaac agccgcccgc acgcaggcag
    21961 aaaaattcac tctcggtgaa cccggcgtgg cgttaaacga tgtgatgacc gatatgcaaa
    22021 aagcctcagt ttctatgcaa atggggattc aggtgcgtaa taagctggtg gcggcttatc
    22081 aggaagtgat gtccatgcag gtgtaggttt tgtaacctgt tgttaattac atccgatctc
    22141 accgcgtggg gcatggatgg ggcaaactca ctcaatttct ggtttaaaat ggatacctga
    22201 tcctgaaagt tttctgccat ccacgatccg taaacgcgat aacatgtttt gtcttcgctt
    22261 tgctcatagg aagagtgaac tcttttgtta gcctggggtt ccttctgatc attattgttc
    22321 ctgcctttat ctcttgccac gccagagccc cctggataca catacaccag gatgaaaacg
    22381 gtgagttgtg ttcaaactgt agtacaattc tctccagtat gaacaggaaa gaatatgcta
    22441 tgaaccctta tatttatctt ggtggcgcaa tacttgcaga ggtcattggt acaaccttaa
    22501 tgaagttttc agaggggttt acacggttat ggccctctgt tggcacaatt atttgctatt
    22561 gtgcatcatt ttggttatta gctcagacac tggcttatat acctacaggg attgcttatg
    22621 ctatctggtc aggagtcggt atcgttctga ttagtttgct ttcctgggga ttttttggcc
    22681 aacgactgga cctgccagcc atcataggca tgatgttgat ttgtgccggt gtgttagtca
    22741 ttaacttgtt gtcacgaagc gcaccgcatt aaaaaataat catccttgag ataacaggaa
    22801 tctgatttgt gcatattatg gctaatgttc ggggcaactt tataattatg attgcatttg
    22861 cgcgatcatt gcgccttttt gcataatgag atcaatgcac atcaaataaa ataacgatag
    22921 gtcacaacaa agaggttttt atgaaaaaac ttatcgtttc aagtgtattg gcattcataa
    22981 cattttctgc gcaggccgca gcatttcagg tcactagtaa tgaaataaaa acaggagagc
    23041 aacttacaac gtctcatgta ttttctggag ttgggtgtga ggggggtaat acatcgccct
    23101 cattaacctg gtctggtgct ccagaaggca ccaaaagctt tgccgtaact gtgtatgatc
    23161 cagatgcgcc tacaggcagc ggatggtggc actggactgt tgctaatatt ccagcaacgg
    23221 taacatattt gcccgctgat gcaggaagac gtgatggtac aaaacttccg actggtgctg
    23281 tccaaggccg aaatgatttt ggctatgcgg gatttggtgg agcatgtcct cccaaaggag
    23341 ataaacccca tcattaccag tttaaaatat gggctctaaa aactgataag attcctgtgg
    23401 attctaattc cagcggagcg ttagttggtt atatgcttaa tgccaataaa attgcaaccg
    23461 ctgagataat acccgtttat gaggtaaagt aggataagag tatgctggca aaagatagga
    23521 ctaacctaaa gattgaagaa atacggatgc ataaacatca tgagattcac agggttaagc
    23581 ctcttatgcc agcttcatgt cgtatccgtc agggaaaaaa agttatcaat tgggagacgc
    23641 atagtctaac tgttgataat aatcagataa tattatttcc ctgtggatat gaattttata
    23701 ttgcgaatta cccagaagca ggtctttatc ttgcagaaat gctttattat cccattgatc
    23761 taattgaaaa gttccaaaat ctttatgcga taaccgatca aattcgtaac acgacaggtt
    23821 tctgcttacc tcagaattcc gagttaatat attgttggga gcaactaaaa acatctattt
    23881 cccgaggctt ctcaacgcaa attcaggaac acttagcaat gggcgttcta ctttcattag
    23941 gagcacatca tgttaattgt ttacttttat cagatagtaa acaatcatta ataagtcgtt
    24001 gttataacct aatgctatcc gaacctggaa caaaatggac agcaaacaag gtagcgagat
    24061 atctctacat ttctgtttcc acattgcatc gccgtctggc aagcgaggga gtaagttttc
    24121 aaagtatatt ggacgacgtg aggttaaata atgcgttgtc tgctatacaa acgacagtaa
    24181 aacccatcag cgagattgcc agggaaaatg gttacaagtg tccttctcgt tttactgaaa
    24241 ggttccataa tcgttttaag ataacaccaa gagagctaag aaaagcgtcc agagagtaaa
    24301 agtattttat gaaggagcaa cagcatcgat ttttattttg gaaaataaat acggcatact
    24361 caacctattg gagcctgaac aatccggtga cttctgcgct aatcggggac gtttataagc
    24421 acatataatt gatactatct tcctccatca caatttcaga aatgcacgtg tgattttttt
    24481 gcatccggcg tttggactat gtggagttca agagtgggaa agttacaaga ggttgagggg
    24541 ataattaaag ggatgcttat gacattagat atccggatag gtacggatat cgttgttatt
    24601 aaatctagtt cgttattatt aaatctagtt tatacgttaa gatgcagcta cagcagaaat
    24661 tgccactgtt aattttttca tcgtgagccc ttttatttga actattattt aaaaatgatg
    24721 tcactgcact acaaatattc atccaatcaa tgagattatt tcaagatgta agttttagtt
    24781 tctcatttaa attgtgaagt agatccctgg atttgcccct atatttccag acacctgtta
    24841 tcacttaacc cattactggc ttgctgccgt
//
LOCUS       DAJTVK010000056        24361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000056 DAJTVK010000000
VERSION     DAJTVK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.056"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="OO372_002278"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="OO372_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO372_002279"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="OO372_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6341755.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO372_002280"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="OO372_002280"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6341756.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="OO372_002281"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="OO372_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="OO372_002282"
     CDS             complement(4104..4388)
                     /locus_tag="OO372_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341757.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO372_002283"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="OO372_002283"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6341758.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="OO372_002284"
     CDS             complement(5289..6497)
                     /locus_tag="OO372_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6341759.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="OO372_002285"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="OO372_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6341760.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="OO372_002286"
     CDS             complement(7292..8977)
                     /locus_tag="OO372_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6341761.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="OO372_002287"
     CDS             9247..9624
                     /locus_tag="OO372_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6341762.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO372_002288"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="OO372_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6341763.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="OO372_002289"
     CDS             10071..10358
                     /locus_tag="OO372_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6341764.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..11064
                     /gene="nfsA"
                     /locus_tag="OO372_002290"
     CDS             10342..11064
                     /gene="nfsA"
                     /locus_tag="OO372_002290"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6341765.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11125..12027
                     /gene="rimK"
                     /locus_tag="OO372_002291"
     CDS             11125..12027
                     /gene="rimK"
                     /locus_tag="OO372_002291"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6341766.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12115..12591
                     /locus_tag="OO372_002292"
     CDS             12115..12591
                     /locus_tag="OO372_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6341767.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12942..14054
                     /gene="potF"
                     /locus_tag="OO372_002293"
     CDS             12942..14054
                     /gene="potF"
                     /locus_tag="OO372_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6341768.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14149..15282
                     /gene="potG"
                     /locus_tag="OO372_002294"
     CDS             14149..15282
                     /gene="potG"
                     /locus_tag="OO372_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6341769.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15292..16245
                     /gene="potH"
                     /locus_tag="OO372_002295"
     CDS             15292..16245
                     /gene="potH"
                     /locus_tag="OO372_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6341770.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            16242..17087
                     /gene="potI"
                     /locus_tag="OO372_002296"
     CDS             16242..17087
                     /gene="potI"
                     /locus_tag="OO372_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6341771.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMVRAEKQRIRDIQ
                     RARHG"
     gene            17147..17635
                     /locus_tag="OO372_002297"
     CDS             17147..17635
                     /locus_tag="OO372_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6341772.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17676..18803
                     /gene="rlmC"
                     /locus_tag="OO372_002298"
     CDS             17676..18803
                     /gene="rlmC"
                     /locus_tag="OO372_002298"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6341773.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO372_002299"
     CDS             complement(18978..19709)
                     /gene="artJ"
                     /locus_tag="OO372_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6341774.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO372_002300"
     CDS             complement(20001..20669)
                     /gene="artM"
                     /locus_tag="OO372_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6341775.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO372_002301"
     CDS             complement(20669..21385)
                     /gene="artQ"
                     /locus_tag="OO372_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6341776.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO372_002302"
     CDS             complement(21392..22123)
                     /gene="artJ"
                     /locus_tag="OO372_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6341777.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO372_002303"
     CDS             complement(22141..22869)
                     /gene="artP"
                     /locus_tag="OO372_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6341778.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(23087..23602)
                     /locus_tag="OO372_002304"
     CDS             complement(23087..23602)
                     /locus_tag="OO372_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6341779.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
BASE COUNT         6003 a         5963 c         6315 g         6080 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc
    10741 aataatattg aagcggtgac gaaactgctg aaattaccgc agcatgttct gccgctgttt
    10801 gggctgtgcc ttggctggcc tgcggataat ccggatctca agccgcgttt accggcctcc
    10861 attttggtgc atgaaaacag ctatcaaccg ctggataaag acgcactggc gcagtatgac
    10921 gagcaactgg cggaatatta cctcacccgt ggcagcaata atcgccggga tacctggagc
    10981 gatcatatcc gccgaacaat cattaaagaa agccgcccat ttattctgga ttatttgcac
    11041 aaacagggtt gggcgacgcg ctaaaaccgc cacgtcgatg tatgatacgc gggcttttga
    11101 ccaggtctga cagagaggtg cagggtgaaa attgccatat tgtcccggga tggaacgctc
    11161 tattcgtgta agcggctgcg tgaagccgct atacagcgcg gtcacctggt tgaaattctt
    11221 gatccgcttt cttgctacat gaacataaat cctgcggcgt cttctattca ctacaaaggc
    11281 cgcaagttac cccattttga cgcagtgatc ccgcgtattg gcaccgccat taccttttat
    11341 gggacggcgg cactgcgcca gttcgagatg ctggggagct atccgctcaa tgagtcggtc
    11401 gccattgccc gggcgcgtga caaattgcgt tccatgcaac tgctggcgcg tcagggcatc
    11461 gacctgcctg tcacgggcat tgcgcattcg ccggatgata ccagcgattt aatcgacatg
    11521 gtcggtggtg cgccgctggt ggtcaagttg gttgaaggca cgcagggaat tggcgtcgtg
    11581 ctggcggaga cgcgtcaggc ggcggaaagc gtgattgacg ctttccgcgg tctgaacgcg
    11641 catattctgg tgcaggaata tatcaaagag gcgcaagggt gcgatatccg ctgtctggtt
    11701 gttggcgatg aagtggtcgc tgcgattgaa cggcgggcga aagagggcga ttttcgttcc
    11761 aatttgcatc gtggcggcgc ggcaagcgtc gccagtatca caccacagga gcgtgaaatc
    11821 gcgataaaag ccgcgcgaac gatggcgctg gacgttgctg gtgtggatat tctgcgtgct
    11881 aatcgcgggc cgttggtgat ggaagtgaat gcgtcgccgg ggctggaagg aatagaaaaa
    11941 accactggta tcgacatcgc gggtaaaatg atccgctgga tcgaacgcca cgctacgaca
    12001 gaatattgcc tgaaaacggg tggttagtcg caatcacatt actgatcatg gttttgcctg
    12061 cgctttttgc gtaagctgtg ccggtctttt tatcgaaaga ggttgtacaa aattatgaca
    12121 tcgctggtcg ttcctggtct ggatacgctg cgtcaatggc tcgatgacct ggggatgagt
    12181 ttttttgaat gtgataactg tcaggctctg catctgcccc atatgcagaa tttcgacggt
    12241 gtctttgatg ccaaaatcga tctgatcgat aacacgatcc tgttttctgc catggcggaa
    12301 gtccgacctt cagccgtatt gccgctggcg gcggatttat ctgccatcaa tgccagttcg
    12361 ctgaccgtga aagcatttct tgatatgcag gatgataatc tgccaaagct ggtggtttgc
    12421 cagtctttat ccgttatgca gggcgtaacc tatgagcagt ttgcatggtt cgtgcgtcag
    12481 agcgaagagc agatttcgat ggtcattctt gaagctaatg cccatcaact gctgttaccg
    12541 actgacgatg aagggcaaaa caacgttacc gaaaactatt tcctccactg ataactcctt
    12601 tcgagcacgc agtcgctggt gcagtggctg cgcgctgcaa aattatctgc tgtttttaac
    12661 cttttcttaa agattatttc acttctcttg tgtcgatttg gctttatcac atagagcaaa
    12721 tacgcataaa aatttgttaa ataccgtttt ttaatccgag ctatagtctc aaaccctggc
    12781 taaagttatt cttgcgatgc ttttatatag cgagcagtgc tggccgggag agagttctct
    12841 tttcttacac cccgccgata aaaaatatgc acgtttattg catatctttt agtgtgacaa
    12901 cttttgttcg tttgttaacg aactttcaga aggaaagaga tatgaccgcc ttaaataaaa
    12961 aatggctttc gggtctggct gcgggtgctc tgatggccgt ctctgtcggc acgctcgcgg
    13021 ctgaacaaaa aacactccac atttataact ggtctgatta tatcgccccg gacacggtgg
    13081 ccaattttga aaaagaaacc ggtattaaag tcgtctacga tgttttcgac tctaacgaag
    13141 tactggaagg caaattaatg gcgggtagca ccggctttga cttggtggtt ccatctgcca
    13201 gctttcttga acgccagttg actgcgggag ttttccagcc gctggacaaa agcaaattgc
    13261 cggagtggaa gaatctcgac ccggagctgc tgaagctggt cgccaaacac gatcccgaca
    13321 ataaatttgc tatgccctat atgtgggcga cgaccgggat tggctataac gttgataaag
    13381 ttaaagctgt gctgggcgaa aacgcgcccg tcgatagctg ggacttgatc ctcaaacctg
    13441 aaaatctgga aaaactgaaa agctgcggtg tctctttcct ggatgcgcca gaagaagttt
    13501 ttgctaccgt gttgaattat ctcggcaaag atcccaacag cactaaagcg gatgattaca
    13561 ccggaccggc aaccgatctg ctgttaaagc tgcgcccgaa cattcgttat ttccattcat
    13621 ctcaatacat taacgacctg gcaaacggcg atatttgcgt cgctatcggc tgggcaggtg
    13681 atgtctggca ggcgtcaaac cgcgcgaagg aagcgaagaa tggagtgaat gtctcgttct
    13741 cgattccaaa agaaggggcg atggcgttct ttgatgtatt cgccatgcct gcggatgcca
    13801 aaaacaaaga cgaagcctat cagttcctga attacctgct gcgcccggat gtagtagcgc
    13861 atatttccga ccatgtgttc tatgccaacg ccaataaagc agccacgccg ctggtgagtg
    13921 cggaagtccg tgagaacccg ggtatttatc ctccggcgga cgttcgtgcg aagctattca
    13981 ctctgaaagt acaggatccg aaaatcgacc gtgtgcgcac ccgcgcgtgg accaaagtga
    14041 agagcggaaa ataatccgca gccttagatg ccgggcgggc gcaccacacc agccggcaat
    14101 tcgcaccatc atggtgcgct tgcacacatt caatgccgga gagcagccgt gaatgacgct
    14161 atccctcgcc cgcaggcgaa aacccgtaag gcgctgacgc cgctattaga aatccgcaac
    14221 ctcaccaaat cctacgatgg tcagcatgcg gtcgatgatg tcagcctgac catctacaaa
    14281 ggtgaaatct tcgcgctgct gggcgcatcc ggctgtggca agtccacgct gctgcgtatg
    14341 ctggcaggtt ttgaacaacc ttctgccggg cagataatgc tcgatggcgt cgatttgtca
    14401 caggtgccgc cttacctgcg ccccatcaat atgatgtttc agtcttacgc gctgtttccg
    14461 catatgaccg tggaacagaa catcgctttt ggcctgaaac aggacaaact accgaaagcg
    14521 gaaattgcca gtcgggtcca tgagatgctc ggcctggtgc acatgcagga gttcgccaaa
    14581 cgcaaaccgc atcagctttc cggtggtcag cgacagcgtg tggccctggc ccgaagcctt
    14641 gccaaacgcc cgaaactatt actgctcgat gagccgatgg gcgcgctgga taaaaagctg
    14701 cgtgaccgga tgcagcttga agtggtggat attctggagc gcgtcggtgt gacttgtgtg
    14761 atggtcaccc acgatcagga agaggcgatg accatggcgg ggcgtatcgc cattatgaat
    14821 cgcgggaaat ttgtccagat tggcgagccg gaagagatct acgagcatcc aactacccgc
    14881 tacagcgctg aatttatcgg ttcggttaat gtctttgaag gtgtactcaa agagcgtcag
    14941 gaagatggcc tggtgcttga ttcgccgggg ctggtgcatc cactgaaagt cgatgcggat
    15001 gcttcagtgg tcgataacgt gccggtacac gtagcgctgc gcccggaaaa aatcatgctt
    15061 tgcgaagagc cgcccgccaa tggttgtaac ttcgcggtgg gggaggtgat acacattgcc
    15121 tatctcggcg atctttcggt gtatcacgtt cgactgaaaa gtgggcagat gatcagcgcc
    15181 cagctacaaa acgcccatcg tcatcgtaaa gggttaccga cctggggcga cgaagtgcgt
    15241 ttgtgctggg aagtggacag ctgtgtggtg ctgacggttt aaggagcaaa gatgagtaca
    15301 cttgaacctg ctgcccagtc gaaaccgccg ggcggattta agctgtggtt gtcgcagctg
    15361 caaatgaagc atgggcgcaa actggtcatt gcgttgctat atatctggtt gatcttgctg
    15421 tttctgctgc catttctgat tgtctttaaa ataagcctgg gagagatggc gcgcgctatt
    15481 ccaccttata ccgaactgat ggagtgggct gacgggcaac tttccatcac tcttaatctc
    15541 ggtaattttc tgcaactgac cgacgatccg ctctattttg atgcttatct ccagtcgtta
    15601 caggtggcgg cgatttcgac attttgctgt ttactgatcg gctatccgct ggcgtgggcg
    15661 gtggcgcaca gtaagccttc gacccgtaat attttattac tactggtgat cctgccgtcg
    15721 tggacctcgt ttctgatccg cgtttatgcc tggatgggaa tattaaaaaa caacggtgtg
    15781 ctgaataatt ttctgctgtg gctgggggtt atcgatcaac cgctgaccat tctgcatacc
    15841 aatctggccg tttatatcgg cattgtttac gcttacgtgc cgtttatggt actgccgatt
    15901 tataccgcgt tgattcgtat tgattattcg ctggtggaag cagcgctgga tctcggtgca
    15961 cgaccgctga aaacgttctt tactgtgatc gtgccgctga ctaaaggtgg gattattgcc
    16021 ggatcgatgc tggtgtttat cccggctgtg ggcgagtttg tgatcccgga actgctcggt
    16081 ggcccggaca gcatcatgat cgggcgcgtg ttatggcaag agtttttcaa taaccgcgac
    16141 tggccggtgg cctcggcggt agcgatcatc atgttgctgc tgctaattgt gccgataatg
    16201 tggttccaca aacaccagca aaaaagcgtg ggagaacacg gatgaataat ttaccggtag
    16261 ttcgttcgcc ctggcggatt gtgattttgc tgctgggctt tacctttctc tacgcgccaa
    16321 tgctgatgct ggttatctat tcgtttaaca gctcgaagct ggtgacggtg tgggccggct
    16381 ggtcaacgcg ctggtatggt gagttattgc gcgatgacgc gatgatgagt gcggttggtt
    16441 taagcctgac aattgctgcc tgtgcggcaa cggcggcggc gatcctcggg actattgcgg
    16501 cggtggtgct ggtgcgtttt ggcaggtttc gcggatcaaa tggttttgcc tttatgatca
    16561 ccgcgccgct ggtgatgcca gatggcatca cgggcttgtc gctgttgtta ttattcgtcg
    16621 cgcttgctca tgccattggc tggcctgcgg accgcggtat gctcaccatc tggctggcgc
    16681 atgtcacttt ttgtacggct tatgtggcgg tcgttatttc ttcaagattg cgggaactgg
    16741 atcgctcgat agaagaagca gcgatggatc tcggtgcgac gccgctgaaa gtgtttttcg
    16801 tcattacgct accgatgatc atgcccgcga tcatttctgg ctggttactg gcttttactt
    16861 tgtcgcttga tgatctggtg atcgccagct ttgtttccgg gccgggagcc accacgttac
    16921 cgatgctggt cttttccagc gtgcggatgg gagtgaatcc ggaaatcaac gcccttgcaa
    16981 cgttaattct cggtgcggtc ggaattgtcg gatttatcgc ctggtatctg atggttcgcg
    17041 cagaaaaaca gcggatacgc gatatccagc gtgcaagaca tggctgaaga cactaaaatt
    17101 tgccaacctg gctacataat gccgcgcatg ccgcggcatt gttttcatgg aagacgaaac
    17161 gttgggattt tttaagaaaa catcttcatc tcatgctcgc ctgaatgtgc ctgcgctggt
    17221 gcaggtggcg gcgctcgcca ttattatgat ccgtggcctc gacgtgctga tgattttcaa
    17281 tacgctgggc gtgcgcggta ttggcgagtt cattcatcgc agcgtacaaa cctggagttt
    17341 aacgctggtc tttttaagca gtctggtgct ggttttcatt gagatctggt gtgcgttttc
    17401 actggtgaaa gggcgtcgct gggcgcgctg gctatatctg ctgacacaaa tcaccgccgc
    17461 aagttacttg tgggcggctt cgctggggta cggttatccg gagctgttca gcattcccgg
    17521 tgaatcaaaa cgtgaaatct tccatagcct gatgctgcag aagctgccgg atatgctcat
    17581 cctgatgctg ctgttcgttc cctcgaccag tcggcggttc ttccagttgc aataatgtgt
    17641 ataatcgtcg cccctgatga tgtgaaggtc aatgtatgca gtgcgcactt tacgacgcgg
    17701 gtcgctgtcg ttcctgtcag tggataacgc agccgattcc agagcaactc tccgctaaaa
    17761 ccgccgatct taaaaatctg ctcgccgatt ttccggttga ggaatggtgc gcgccggtgt
    17821 caggcccgga acaagggttt cgtaataaag ccaaaatggt ggtgagtggt agcgttgaaa
    17881 aaccactgct cggtatgctg catcgcgatg gcacgccgga agacctttgt gactgcccgc
    17941 tttatcctgc ctcttttgcg cccgtttttg cggcgctaaa accgtttatc gcccgtgcgg
    18001 ggttaacgcc ctacaacgtg gcgcgtaagc gtggcgaact gaaatacatt ctgctgactg
    18061 aaagccagag cgatggcggc atgatgctgc gctttgtact gcgttctgaa accaaactgg
    18121 cacaactgcg taaggcgctg ccgtggttac aggaacaact accgcaactg aaagttatta
    18181 ccgtcaatat tcagccggta catatggcga ttatggaagg ggagacggag atctacctga
    18241 ctgaacaaca ggcgctggca gagcgtttta atgacgtacc gctgtggatc cgtccgcaaa
    18301 gtttcttcca gactaatccg gcggtcgcca gccagctgta cgctaccgcg cgcgactggg
    18361 tacggcagct gccagttaaa catatgtggg atctgttctg cggtgtgggt ggctttggtt
    18421 tacactgcgc gacgcctgac atgcagttaa ccgggatcga aattgcatca gaggccattg
    18481 cctgtgcaaa gcagtcagcc gctgaactgg gcttaacgcg tttgcaattt caggcgctgg
    18541 actccactca gtttgccacc gctcaggggg atgtgccgga gctggtgctg gttaacccgc
    18601 cgcgccgcgg cattggtaaa ccgctgtgtg attatctctc aacgatggca ccgcgtttta
    18661 ttatctactc cagctgtaac gcccaaacca tggcgaaaga tatccgcgaa ctgccaggtt
    18721 accgcatcga acgggtacag ctttttgata tgttcccgca caccgcgcac tatgaagtga
    18781 tgacgctgct ggtgaagcaa taaaaaagcc gcaggtgcgg cttcagattg ctgatcaggt
    18841 gcgctttgtt catgccggat gcggcgtgta cgccttatcc ggcctacaaa atcatgctaa
    18901 atcaatacat tgcagagttg atgtgggcca gataagcgca gcgcatcagg caatcttact
    18961 tttgtcatca gtctcaatta ctgcgggaac cactggtcat tgattttctg atatgtaccg
    19021 tcagctttaa ttgctgccag cgcgttattc agtttttcca gcagagcttt gttatccgga
    19081 cgtacagcga tgcccagacc ggtgccgaag tattgcggat cggtcacttt ttcagtagca
    19141 acgcccagtt gcggattggt tttcagccat tcgtttacca ccgctgtgtc accaaatacc
    19201 ccatcaatac gaccattttt cagatcgata aaggcattct gataactgtc ataggagaca
    19261 gttttcactt cggggtgctg atcctgaata tatttctggt gcgtggtgcc gttttccatc
    19321 ccaatacgtt tgcctttcag atcggcaaac gttttgtagg tatctttttt ggcaatcacg
    19381 acggctgagt tttcatagta gggcgtggta aacgatacct gcttgctacg ctcgggcgtg
    19441 atatccatac cggagattac ggagtcatat tttctgaatt tcagtgacgg gatcaggctg
    19501 tcgaacgcgt gattagtaaa agtacattct gcctgcattt gtttgcacaa ggcttttgcc
    19561 agatcgatat caaagccgac aatctcatta ttagcaccta tagattcaaa gggtggatag
    19621 gtggctgaaa cgccaaaatt gattttctct gcggcagaag caccgaaagt aaaggaagca
    19681 agtaaagcgg caagaactaa ctttttcatg atggaactcc cgtctgtcaa tcttatgatt
    19741 tttggccgtg tctgcggcat gggataacaa tgccatcaag tgaatttata tgcaataaat
    19801 atgattaaat aatttaaatg aaataaaaaa agacggataa cttagtgagt tgtccgtctt
    19861 cattataaga atttatgcac tatgtaggcc ggataaggcg tccacgccgc atccggcaca
    19921 ggcaccgtgc tgatgtctga tgcgacgctg gcgcgtctta tcagacctac aaaccccccc
    19981 ggcgaatgta cgcagctaca ttaattccgc cgttcgaatg ccagtgcttt gcgctcgatc
    20041 agacgcatca tcagcgtcag caggccgttg acgaccaggt aaataatccc tgccgcaccg
    20101 aacaccatta catcgtaggt gcgtccgtac aacaactggc tgtatcccat cacttccatc
    20161 agcgtaatgg tgtaagccag agaggtactt ttgaacacca gcaccacttc gttggaataa
    20221 gaagagagcg agcgtttaaa ggcatacggc agcaggagcg ccagcgtatc ttttttgctc
    20281 attcccaggg cgctacagga ctgccactga ccttctggga tcgcacgaat tgcaccgtaa
    20341 aacagctgcg tggtatacgc cgcactgttc agcgataacg caatcagcgc acataaccac
    20401 ggttctgaca acaaatgcca cagtgccgga tactcctgca acgtcggaaa ctggcccggc
    20461 ccgtaataaa tcaggaagat ctgcaccagc agcggcgtac cggtaaacag cgtgatataa
    20521 ccccgcacca gccacaccag caccggcgtt ttcagcgtca ggatgatggt aaaaatcaat
    20581 gccagaatca gtgccacaat cagcgaggca acggttagcg tcaggctggt gtgtagccct
    20641 ttcatcagtt cgggtaaata ctcaaacatt agctgggcct ccgctcaaaa cgtgtcgcgc
    20701 gcaggtcaat gcgtttgaga atgtactgac tgagcagggt gatcaccagg taaatcgccg
    20761 ccgccacaat gtaccaggta aatggttcct gggtacgagt agcgatgctt tttgtttgca
    20821 gcattaaatc attcacacta atcaaactga ccagcgcggt atctttcagc agcaccagcc
    20881 actggttacc gaggccaggc agcgcatgac gccacatctg cggcatcacc agacggaaaa
    20941 agatagccga tttcgacagc cccagcgcct gaccagactc ccactgaccc accggcaccg
    21001 ctttcaacgc gccccgaagc gtttgcgagg cataggcggc atacagcagt gacagagcga
    21061 tgacaccaca aaggaacggg ctaacgtcga agttctcaat gtccatctgc actgggatct
    21121 gcacgaaccc aagattgata gtgaagccat ccgaaagcgt cagcagcagc tgcgaggagc
    21181 caaaatagat aaacagcacc accagaattt ctggcaggcc acgcagaatg gttaccagcg
    21241 ctgaacctgc ccacgcgaca ggacgccatt ttgccgactc ccataccgca aagaacatcg
    21301 ccagcgccag cccgacaatc aatgcacaaa cggcaaggcc gacggtcatc ccggcggcgc
    21361 ttgctaaagg aaaaaattca ttcatcagga attacttctg gaaccatttg ttgtagatgg
    21421 tttcgtaagt gccatctttc ttcacttttt ccagcgcagt gttgagtttc tgctgcagct
    21481 cagtgttgcc ctgacgtacc gcgatgccga ggccagtgcc gaagtaatct ttatcggtca
    21541 ctttgtcgcc caccgccgcc agtttcgggt tatctttcag ccactcagtg accactgcgg
    21601 tgtcaccgaa gacgccgtcg atacgcccgt tttgcagatc cagttttgcg ttctggtagc
    21661 tgtcatacgg aacggtagtg atttccgggt gcttatccat aatgaatttc tggtgtgtcg
    21721 tcccgttctg tacgccgact tttttgcctt tcagctgatc aacactggtg tatttgcctt
    21781 gctgacccac aaacagggca gagttgtcat agtacggggt ggtaaacagc acctgctttt
    21841 cacgctccgg agtgatatcc atgcccgcca tcacggcttc tacgcgacgg aatttcaggc
    21901 ttgggatcag gctgtcaaac gcctggttag agaaagtgca ggttgcatca atctctttac
    21961 acagcgcttg tgccaggtcg acgtcaaaac caacgatctg gttgtttgca tcaatcgatt
    22021 caaacggagg ataggatgct tcggtagcaa aacgaatggt ttcggcagct gtggcggaaa
    22081 gactaaaacc tgcaattaac gcggcaatca gaactttttt cattgttgtt atcccgaatc
    22141 ttagtgagag agatagtttt taaatgcttc ggtttgcggc tcggtaaagc agctcgcgtc
    22201 gccttgttct acgatatgac cattttccat atacaccact cggctggcgg ttttacgcgc
    22261 cacttcaact tcgtgggtga cgatcacctg ggtaatattc gtttctgcca gctcacgaat
    22321 gatgctgacg atttgtgccg taatttccgg gtccagtgcg gcggtcggtt catcgaacag
    22381 cagtacctgc ggttccatca tcaacgcacg ggcaatagca acacgctgct gctgaccacc
    22441 agaaagatgc agcgggtaac gatcgctata aggtttgaga cgcagacgtt ccagcagttt
    22501 ttctgcacgg gccagcgcct gatctttact caaccccagt acacggcagg gcgcttcaat
    22561 caggttttgc tgcacggtca gatgcggcca caggttgtat tgctgaaaca ccatgccaac
    22621 gttacgacgc aaatcgcgaa tcgctttgtc agagggtgtt ttggtgaaat cgaaatggtt
    22681 gcctgcaatg ttgagcgtac cggagcgtgg catctcaagc agattgagta cacgcagcag
    22741 cgagctttta cccgcgccgc tggggccaag taacaccagc gtttcgccct gtgggcaatc
    22801 cagcgtgata tcgaacagcg cctgatgcgc gccgtagaag caattaatgc cgtttaattg
    22861 aatactcatt gacactcgta tactggcagt ctgatagcta ttgaggtcga agatagtacc
    22921 tttgacagaa taattatgca atatttctgc tttaaaagtt aaaagcaaag cgcattattc
    22981 aataaacata gcacaaaata acgggggcgg tggtcggcga gcataaatgt cggcattcct
    23041 cacgaaatgc cggacaattt acggggttta ttggttgatc aaggcgttag cgattctcga
    23101 tggactgacg gagcgtaccc gccgtggcat gaacgctgcc gcctaagtag cgcacatcgt
    23161 cgatgaccca acactggcct tcctgaatca ttaacacttc atcctgccaa ccctggtcac
    23221 cctgtttgag atctacgcgc aatggaatgt tacgggcatc acgattaggg atagtcgatg
    23281 cactggcaac gtgggcgcta tctggcaagg tggttcgact ggagaacgga tcgctgctta
    23341 acaattcacg atgactatta tcgcggctgg catcgctaag aagggttgcc agtttgtcac
    23401 tcaaataggg gcgcagggcg gtaatgtcat tgctgcgatg ttgaatgcgg tagtcataga
    23461 attgttgagc cacgctgtca ggccccccct ggacacagga accggtgcgg ggaccgttat
    23521 ctttataagc tggcgtgacg gtggtacagg cactcagcag cagtgcacat ggaataagta
    23581 gagatagtga acggtagcgc ataatcattt ccttattagc ttgctgtctg cgtcacacta
    23641 cgaccaaacg ctatcagatt atttaataca ttgtttttaa tacaattctg aaacaatctg
    23701 aacacctgat tgggtttgag tccattgctg gctatccact caaggttgct cgtagctgac
    23761 ttacttaaat tacccaaaca atagacagtg taaaccattt gtgaggttga gcgtgttata
    23821 tggctgaaat gatcatttaa ggtaaatatg atagttctga aatggtggta taaatcctcc
    23881 tgtagaggtt aagagcatct gcaagaagcc agtttcacat ttataacgcg tgataaatga
    23941 acgcggttat tgtctaatca ttacaaaatt tttcacatgc gataagagat cctgcgatta
    24001 agatgattgc caaaattacg ttgtattttt gaaaatgagc gaaatttgat agtcccagct
    24061 ttgactctcg ataatattat ttatgctaca attgtggcta aagaatttaa gctaatgaat
    24121 tttagcgaag aataaattaa gttgtttcag tatctcgtta attaatatga atataaaagg
    24181 cgttgtgctt ttataattat aaagcacgag gagttcggtg gattatcgtt tttcttgggt
    24241 gatttttaat atgataaaca atatatctga tcaagcatca tcatttccag gcacacaact
    24301 aaatcaaagt gataatttcc tggattcact gagggaattt ttcgcgatat taaattccag
    24361 t
//
LOCUS       DAJTVK010000057        24278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000057 DAJTVK010000000
VERSION     DAJTVK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.057"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..200)
                     /locus_tag="OO372_002305"
     CDS             complement(<1..200)
                     /locus_tag="OO372_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6341780.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAV"
     gene            complement(203..1870)
                     /locus_tag="OO372_002306"
     CDS             complement(203..1870)
                     /locus_tag="OO372_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6341781.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(1867..3975)
                     /locus_tag="OO372_002307"
     CDS             complement(1867..3975)
                     /locus_tag="OO372_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6341782.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(3962..4783)
                     /locus_tag="OO372_002308"
     CDS             complement(3962..4783)
                     /locus_tag="OO372_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6341783.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO372_002309"
     CDS             complement(4942..6771)
                     /gene="glmS"
                     /locus_tag="OO372_002309"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6341784.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO372_002310"
     CDS             complement(6933..8303)
                     /gene="glmU"
                     /locus_tag="OO372_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6341785.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO372_002311"
     CDS             complement(8654..9073)
                     /gene="atpC"
                     /locus_tag="OO372_002311"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6341786.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO372_002312"
     CDS             complement(9094..10476)
                     /gene="atpD"
                     /locus_tag="OO372_002312"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6341787.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO372_002313"
     CDS             complement(10503..11366)
                     /gene="atpG"
                     /locus_tag="OO372_002313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6341788.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO372_002314"
     CDS             complement(11417..12958)
                     /gene="atpA"
                     /locus_tag="OO372_002314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6341789.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO372_002315"
     CDS             complement(12971..13504)
                     /gene="atpH"
                     /locus_tag="OO372_002315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6341790.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO372_002316"
     CDS             complement(13519..13989)
                     /gene="atpF"
                     /locus_tag="OO372_002316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6341791.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO372_002317"
     CDS             complement(14051..14290)
                     /gene="atpE"
                     /locus_tag="OO372_002317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6341792.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO372_002318"
     CDS             complement(14337..15152)
                     /gene="atpB"
                     /locus_tag="OO372_002318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6341793.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO372_002319"
     CDS             complement(15161..15541)
                     /gene="atpI"
                     /locus_tag="OO372_002319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6341794.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO372_002320"
     CDS             complement(16158..16781)
                     /gene="rsmG"
                     /locus_tag="OO372_002320"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6341795.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO372_002321"
     CDS             complement(16845..18734)
                     /gene="mnmG"
                     /locus_tag="OO372_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6341796.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO372_002322"
     CDS             complement(19113..19556)
                     /gene="mioC"
                     /locus_tag="OO372_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6341797.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO372_002323"
     CDS             complement(19646..20104)
                     /gene="asnC"
                     /locus_tag="OO372_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6341798.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20256..21248
                     /gene="asnA"
                     /locus_tag="OO372_002324"
     CDS             20256..21248
                     /gene="asnA"
                     /locus_tag="OO372_002324"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6341799.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO372_002325"
     CDS             complement(21253..22704)
                     /gene="viaA"
                     /locus_tag="OO372_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6341800.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO372_002326"
     CDS             complement(22698..24194)
                     /gene="ravA"
                     /locus_tag="OO372_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6341801.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         6003 a         6312 c         5775 g         6188 t
ORIGIN      
        1 acagcgtaac gtcctgtttg atgtagatgt cttgctatca cacctaaatg cgagggcaga
       61 cctaaggtag cgaccacctt gcggttgcca tacacctcat ccaacagctg cttaggacta
      121 acaatccctt gataaacgcc tgcccgtgca atagtgctat aaatcagctc attcgagtac
      181 ggaacaggaa agtttctcat ggctaacctg cttgtttaaa taagctcgcc atatccacta
      241 ttaccccttt cccttttaac cgttcatgca tggttttttc aggttggcgt tgtgaataga
      301 tataacgtaa atccgtatca ggcaagctat cccagtcgct gggcttgact acctttatag
      361 ccgaaacctt tttactcttg gagggctttt ctagttctga cactaccgtt tcgccttcca
      421 tcaaccactg caaaacaaga ggcagtaact tttgtcttgt catcgttgga ttctggctaa
      481 acgctttttt aatagtggga attaacaggc ttgaatcgta atcctctttc agcatcagat
      541 ataaatgacg ctgatcttcg gtatctaact cttgaagggc tttttcttct ggtgtttgtt
      601 cttgtatcgc tgcgatatct agctgaagtt ggattaaccg tttatcaatc tcgggaacga
      661 ctagatcaga ataacgagca atgcgttctg ggatacccga gcgtaatgcc tctagcatgg
      721 ggtgcacagg ctttaactca tcttgataca cttgccgcaa taaaccagcg gtaatacgct
      781 cattgcctaa agctagcgca cggagctgag cgagtacaaa aagttttact acaatgtcca
      841 tcactccttg gcttagctca taccacacat cacggacctc atccgataac agcgcatctt
      901 tgcgttgtaa aagctgtaat tgccaaagat tatccgtaaa agcgatccac tcttgattgg
      961 gctttccacg ttgcgtttgt tgtataggat cccagaatat agctccaaac cctgcccctc
     1021 tacgtgcaga ccgcaaatca gcctcaaaaa tctctcgtgc tttaggggta ccaatcaaca
     1081 tcactggtac gccaataata ttcaccatcg tcacaaaaaa gttcagcatc tcttgagatc
     1141 cacccgaacg agagcggctt aaatgctgaa tttcatcaat aaccaacaac cctaaagcat
     1201 gtgcattggc tatttgcgac atcaaagcca acatggtttc tataccatga cgttttaagc
     1261 cataacgacg ctcatagttc gagcccaagg ctcgatccaa cgctctgaaa aaattcaagc
     1321 agatttcttt tagcgaacca ttatgcgagc agtctatttt caaatacacc acctgctcta
     1381 cattgagttc acgatggtaa atcacctgag gatacgtggc tagaatacga tgaagagagg
     1441 tcgtcttccc actaccagaa caaccaatta acaataagct ttgtgccgta gatcgtgcct
     1501 cctcaaagcg aaatgtctcc aactctcccg tttgaacacg ctcataacca ttttgtaaat
     1561 gcttttgtaa atctcctgtt ttaggatttc tgcctacgta gccacctcga atcatgaccg
     1621 aaatacgctc acttagtagc aaatgcgtac ctaatggctg aaaatagtca tctggaatac
     1681 gacaaatggt atgagctctg ataacacggg acttttgcaa gtcagaggaa gtaagctgta
     1741 aagaggattt cagtgatgca gcactattca ctgactcttg taatggtggt aaggcctcga
     1801 taaaaggatt atcacgataa gcctctaccc ccgtatcacg ataaactgct tgaatccggg
     1861 tagcactcat gactcatcct tttctggtgg atcctgaaat aattcaggca cgtatgtggg
     1921 taggctgtaa tcttcttgat catccgcttc cactgcgttg aaaggaataa ctttagcctc
     1981 atcacctgaa gagcttggct tcaaatgctc cgcacgtttt ttacgctccg aggtcacggc
     2041 ttctttttta ttagttttaa tctgcttaat gcgtgttgat ttgggctcag tagtactggg
     2101 cgttaactta ttcgctttct gaatggtttg ctgaataaac gcctcaagct ccctgcgttt
     2161 agttaactca tcctgcttcg cattggcttt attgtgtttt tcttgtgctt gtatatccca
     2221 aacctcccaa aatgagagac ctttaaactg ccgactacgt tccgtcagat tacagcgcca
     2281 aaatacacgg ctgccaactt gcggaaacaa ataaatcgta tcaaccagca ctgggtcata
     2341 agccgcttct aaatgttgag gtctagctat atcagtgctc cgctgcaacc aaccctcacg
     2401 cagaatctct gaccccgagt aatacaaacc ccacaaatta acgccaaatg aagaaataga
     2461 gacctttcgg cgaggcagta acgctactcg caactgctct tgctccacag cccttaaact
     2521 acctgtacga tgctgcatac cccattgcca tagctggaca ggaatagacg gtaaatctgt
     2581 aggaaaatca gcatctcgat cgtatttatc catcaccaga tggttatttc tgaataagat
     2641 cgtacgcaaa ataatttgtg tgaactcaaa taccgacaga gatgcatcta acctatagtc
     2701 tgtttcacca tggcttttga tccgactgcc ctctacaatg ccaggtgcaa aggacttaaa
     2761 ctcggcttgt agtgttctaa aagtgctttc cactatgcct ttagcatcgc cacgtctagg
     2821 tggagcactt tccactcgca cattaaaact agaaactaag gcttcgacct gatgactcat
     2881 taattcgcca cggtccgcta gcaacacatc tggcaaacct acacacggcc agtctgagct
     2941 actaatctca atatcatgct gggcacaaat ggccgttttg tcagagcaag catttacaaa
     3001 agcctgcatc gccaccacat aagacggatt ttcaaagccg atataaaagc ccgtgatcat
     3061 ccgactaaac acatcaatca caatgtaaag cgttggtctt cctatgattt tttggcgatc
     3121 atgatgatcc actaaataaa tatcagcaat cgtggcatca atctcataac gactcccagg
     3181 gcctaacgcc tgagaagtgg ctgtactact taagggtcgt acgtcttttt tatatacccc
     3241 tgctttaact ctagacttta agcgctgagc tttagggtat tctcgatcat aaaaataacg
     3301 aaactgacgt agtgttgggt aatcctcttg gggaatgcga ggaaaatact gagcaaacaa
     3361 gtccacaaat cgtctatagg caacggtggt ctttgtacct ttttgattta acaggtgctt
     3421 ttctatggtc aacctaaaaa ggcgttcaat ctcgggcgtt accttggttc cttcaccctt
     3481 accatattct ctggctcggc caatctttgc tgttcctgtc gctgaacgtc tttcccctgg
     3541 tgcaccgctg tttttgtagt caggaattaa tgcattaggc gtttgaccac gctgccagta
     3601 acggcgtaac aacttataaa ctgtagcctt agtaacctta tgttcttgga ccacatgctc
     3661 aacgagttcg cttctgactt tagggtcgaa acgatcctta ctattaataa taggaagaat
     3721 ttttcgataa tcctcctcgc gcttctggaa gctgacagaa tctacagacg gctcttctaa
     3781 atcaagatgt acataagggt catcagttct aactaagcgg ccttcatcta aatactgcat
     3841 tagcaacaac tcagccctag cttgtggaac tcctttatca gcgcttattt gcatccagac
     3901 aacttggcca tcctctatag ccaaaatgcg atacggatca ttatcaaata gcacaacctc
     3961 attaatttgc cacatagcgc aactcctcca tattcactac ttggctaata cagagatctg
     4021 cacacttatt tgccctgaaa gacttataaa tattgaactt aataaaacca tttgcggtta
     4081 aggctcgtat ctcactcaat gttttgccta actccaaatc ataagcaata tcaacctgct
     4141 tacagacatt gataatgttt tcatctcctt tttcttgcag gatatgggcc aatggggata
     4201 gttgtgctaa aagctccgca gaaacttctt ctgttttcac tgaataaagc cattcaatat
     4261 tttcttttac tacgggattt atttctttat cagtaaaaat gaaccaagga atttgctttt
     4321 gctgccaata gcgacgctct agttctagtt tttctaaggt acgctcgtct tgtaaggctg
     4381 ctgcaggttt gacttgaata gcaaactgct caaaaggacc atctttgcag tccactaaaa
     4441 aatcagtaga cataacctga tctacaccac gaataacagg atgcttaata ccactatcta
     4501 ttgcaatctg cctggtatca ctaggtaata aggggaactg ctcgcgtata tctagcacgc
     4561 tgctctccca ctcaagactg agaaaaacag caagctctaa gtcagatagc aaatgatgga
     4621 ctcgtcccgt cttatgagaa taaatacggt gggaacgacc tgaagaagga acttcttgta
     4681 ctgttagcca tggaatatag tctttaccat gcccttggcc acgcccctct ttaatacgac
     4741 gggcaatttg cacttcagaa aatgaagagt ttgctttagc cataacaaaa gtccagtatg
     4801 ctttttcaca gcataactgg actgatttca gtttacaact attctgtcta gtttaagact
     4861 ttattgtcat agtttagatc tattttgttc agtttaagac tttattgtcc gcccacagcc
     4921 cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaggt tacgcggctg
     4981 gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca gcggaacggt
     5041 gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt gcatgttatc
     5101 gctacttaca aaacccgcat cctgatcggc gaagacatac aattggccgc cacgcgcgcg
     5161 aacttcttca atgttggatt tcagtttttc cagcaattcg ttgttcggtg caacgacgat
     5221 aaccggcata tcggcatcaa taagcgccag cggaccgtgt ttcagttcac cagcagcgta
     5281 ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca gtgcgattgg
     5341 gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat cttctgccag
     5401 agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca gcgcctgcag
     5461 accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag acagcttcgc
     5521 caccagcatc aacagcacag ttaactgagt ggtgaatgct ttagtggacg ccacgccgat
     5581 ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag aagaacccgg
     5641 aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac gcaggcccgc
     5701 cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt tacgacgcac
     5761 ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa tacctgccag
     5821 cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac aggcgaggat
     5881 ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct cgcttaaatc
     5941 aacctgaccg tggctgatgc gcccggtaag ggtgtttttt atcgcgttcg gctgttcgta
     6001 gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat attgcagatt
     6061 ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt ttaccgaacg
     6121 gcgagtgatt tccgcaatat cgccctcttc aaggaagata aagcgacggg tcaccggcaa
     6181 cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaggccaa tcaccagcgg
     6241 actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga tcactgtacc
     6301 gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag tcccgccttg
     6361 tttcagctcc cagttcacca gatgggcaat cacttcggtg tcggtttcag aaacgaaggt
     6421 atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga tgccgttatg
     6481 caccaccaca atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag gttcaccgtg
     6541 ggtcgcccag cgagtgtgag caataccggt gccgccatcc agaggatgtt cttccgctgc
     6601 ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac cttccgcatc
     6661 aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta aaccttcaag
     6721 aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca tagtttttga
     6781 ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg ccccgagcct
     6841 tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta tgttatctcc
     6901 tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg acgacgccag
     6961 ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc gacattacgc
     7021 gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg ggccaccagc
     7081 tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt attcgcacca
     7141 tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat ttccgcatcg
     7201 cccaggtaag tcagatgccc gactttcgag cctttaccca gacgcgcttt tttcatctca
     7261 acgaagttac caacgtgtgc accttccagc aactcagcac caggacgcaa acgagcaaac
     7321 gggccaatag tacaggccgc tgccagattc gcgtcttcca caacggtata cggactgatt
     7381 tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat tttcacgcga
     7441 tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac atcgcgcccg
     7501 tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac gcctgctaac
     7561 agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag ttgcaggcgg
     7621 ttattcacac cttctacttc gcttaaacgt tgcggatgaa cggcgacgat ttcacgccct
     7681 tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc attattgttg
     7741 gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat gccggtgttg
     7801 atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat gccggtaact
     7861 ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt caccgtcagc
     7921 agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc gacagagatc
     7981 agcggcacgt cgccgtagag cattaaaatg tcttcatcat cggcaaagaa aggtgcggcc
     8041 tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt aaggttgtcg
     8101 tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaagtg aacgtgcgct
     8161 gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc ggcaagggta
     8221 tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc aaggatcact
     8281 acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg aatttaaacc
     8341 gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga taaattgttc
     8401 aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac gccttttgtc
     8461 tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt gctcttattt
     8521 cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct atgtgaacgc
     8581 tcttcaggac ggatcacgcg taaaaaaaag ccagcctgtt tccagactgg cttttgtgct
     8641 tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc tgcgcgatcg
     8701 ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag ctgctaatgt
     8761 gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc tggccgcgaa
     8821 ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg ccgccagaca
     8881 gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca ggcttaatgg
     8941 cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta cccgttacct
     9001 ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg tccaggtggt
     9061 aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt tttccacagc
     9121 ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt attcgccttc
     9181 catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac ccggagaacc
     9241 ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct tacgagcacg
     9301 cgctaccacc agtttgtctt cttcagacag ttcatccata cccaagatgg cgatgatgtc
     9361 tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt cgtagtgttc
     9421 ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt caacggccgg
     9481 gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa ggtgcgcaaa
     9541 ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg cctgtacgga
     9601 ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca tctcttccgc
     9661 cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg atacttccgt
     9721 accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac gaccttcgtc
     9781 acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt ttcccggcgg
     9841 ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt cggtcatttc
     9901 gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca cagagtaacc
     9961 ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt tacctacacc
    10021 cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca ggtcgataac
    10081 tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg aaggtgctgc
    10141 gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga ccggttcacc
    10201 cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga tcgggtgttc
    10261 gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg cgatggtacg
    10321 cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat taccattttg
    10381 cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga cgtcaactac
    10441 ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac gaaataacct
    10501 gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat gctggcctga
    10561 cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt gtcggtcgcg
    10621 gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac cacgccctga
    10681 taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt cggatcgggt
    10741 tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc cggtaacggc
    10801 agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt gctgacaatg
    10861 taaagcttgt ccagacggcc ttcgtcgtaa gcctgcaaca tcacttttac cggaccgatc
    10921 agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac attgccgccc
    10981 acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca ttgaacgctt
    11041 ttgtcggtcc aggtcttcat ttccgccagt agttttttga acaggttaat gttcaaacca
    11101 ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac gtcgcggtct
    11161 tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat cactttgcgc
    11221 atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg cattttggaa
    11281 gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct tgcgatctta
    11341 ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg cctgccctaa
    11401 ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg atgcctttca
    11461 gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc atcaacggag
    11521 cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt ttcgacagtt
    11581 caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc tgcgcaacgg
    11641 acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg tggtcaagct
    11701 gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc agttcacgat
    11761 actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg gtctgtgctg
    11821 caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata ccggcgttga
    11881 acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg gtcggaacga
    11941 acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga gaaccggttt
    12001 tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca cgtgcagcac
    12061 gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca cgtcctggcg
    12121 gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac aggtcatcgt
    12181 aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg caaccggcat
    12241 acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca acgatggtgt
    12301 tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg gacgctttct
    12361 ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg atggcatcga
    12421 tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc tgaccacgac
    12481 cgattgggat catggagtca acggctttat aaccggtctg taccggctga tctacggact
    12541 gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg tgatccagcg
    12601 gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc aggccacggc
    12661 caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg gcaaggtcag
    12721 cgtacggacc cataacaacc gcacctacag agtcgcgctc aaggttcagt gcgatagcgt
    12781 aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg ccgtgaatgc
    12841 ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca ctcacaacat
    12901 tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc agttgcatgc
    12961 tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg cgtacgctgc
    13021 catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac ttatcgattt
    13081 tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa attttcgcga
    13141 gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta gcctcactca
    13201 cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga cgaccatttt
    13261 cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca caaactgcga
    13321 taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct gccatttgtt
    13381 cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct acactttggt
    13441 gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta ataaattcag
    13501 acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt cgctgttagc
    13561 agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca ggatagcaac
    13621 ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg cctgcgcctg
    13681 ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca gaatctgcga
    13741 gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt ttttcagctg
    13801 gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg cggaagcaag
    13861 gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg gccatacgta
    13921 cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga ttgttgcgtt
    13981 aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct ttaaataaaa
    14041 gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca gcgatcatcg
    14101 ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga ggaatcagat
    14161 caggttgacg cgctgcgcct tccaggaatt tacccccgag gatgccgata ccgatcgcag
    14221 caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga tccatattca
    14281 ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt ggtaaattaa
    14341 tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat gaaggcttgc
    14401 agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca ctgtgaccac
    14461 cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat gttaccgaac
    14521 agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag gattaagttg
    14581 acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt gaagccgccg
    14641 atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccag tgccatagac
    14701 agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc cagtacatgt
    14761 tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat caggaatacc
    14821 cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg gtacatgtct
    14881 ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa cttacctggc
    14941 acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa cagacccagc
    15001 accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg ggggttttgt
    15061 ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg gtgtcctatg
    15121 taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg ttgttaatta
    15181 cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc agcggcaaga
    15241 ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc agaactttga
    15301 aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta tacgcctggt
    15361 gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg cccccgctta
    15421 ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt gctatcacca
    15481 ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc gacacagaca
    15541 tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa actttcttta
    15601 aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc gtgatttcaa
    15661 acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca tacaattgcg
    15721 acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa acactagtga
    15781 taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagtatattt atttacttgg
    15841 caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact gttttaataa
    15901 atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt aacagccaat
    15961 gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact tattttctgc
    16021 ttaccaaaaa aagccacgtt atcttgttga tacaaaagag tgaacgtggc gttaaatgta
    16081 accagttata tcagtagaaa acttggttgt tgttaacagt ctaaccggtc aattttttat
    16141 gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg acgttcgcca
    16201 tccagagctg gaacctgaag tttaaccact gattcgacct gatattcttc gggcaacaaa
    16261 gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg ctcaccagga
    16321 agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct aattacgcca
    16381 tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggctc aatattctcc
    16441 agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag gctatccaac
    16501 agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag tcctggtccg
    16561 gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat gctatcgaga
    16621 atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta cgctttgttc
    16681 catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc ggtaagcgaa
    16741 atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac ctgttcttga
    16801 tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc gctacgacgc
    16861 agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg cgtgacgcca
    16921 gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt ggcgatcact
    16981 tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag ggtgttctcg
    17041 ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc gtatttaacc
    17101 tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa cggcgtcagc
    17161 gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc actggcttca
    17221 cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc cgacggggtt
    17281 acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt ctcgttaaag
    17341 cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt caaacgcaga
    17401 tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa catacgatac
    17461 ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata cgcctgagaa
    17521 cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag accgaccagc
    17581 aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc agcaaagaac
    17641 agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc gaagaagtca
    17701 tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg catggagcgg
    17761 acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg ataaatttcg
    17821 ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc ggcgaagcgc
    17881 atgactttgt cttcgatcga tgggcagtag cgtgggccga caccttcgat cacccctgcg
    17941 tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc gttggtatga
    18001 gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa cgagaatacc
    18061 ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt tcgagcatca
    18121 atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc acgcaaacgg
    18181 cgagaaagcg gaatggacgg cggatcacca gcacgaccac cgctgtaatt atccagaccg
    18241 atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt ggcacggaac
    18301 ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat aagatcttca
    18361 accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg taccgcctga
    18421 cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc tttgcttgcg
    18481 tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag accgccgagt
    18541 gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg gttgcagctc
    18601 atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc catacgcgcc
    18661 gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac gtcaaaagga
    18721 tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat tgaagcccgg
    18781 gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg ggtattaaaa
    18841 agaagatcta tttatttaga gatctgttct attgtgatct cttattagga tcgcactacc
    18901 ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc attaactgtg
    18961 aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta tacacaactc
    19021 aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct tcctgacaga
    19081 gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat ccacgatccc
    19141 agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat cttcagtgtt
    19201 tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc gatagcccca
    19261 caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac cgcagaaaga
    19321 tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg aatatctccg
    19381 gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc ttccaacagc
    19441 ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc caggtgttct
    19501 gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc tgccataaac
    19561 cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat ctacctgtgg
    19621 aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc gggttttgca
    19681 ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg ttgataagta
    19741 catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg tagtggcctg
    19801 ttgtgtaata ggcttcagtg acttcagcaa ggctttccag ctttgccagc gcggaagggt
    19861 agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa ccgagctgct
    19921 tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt ttctctactc
    19981 gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg taagcggtgc
    20041 gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc agataatttt
    20101 ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct tttttaatga
    20161 atcaaaagtg tgttaggctt tttattgaat gattattgca tgtttgtcgg tttttgttgc
    20221 ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac attgccaaac
    20281 aacgccaaat tagcttcgtg aaatctcact tttctcgcca actggaagaa cgtctggggc
    20341 tgatcgaagt ccaggcgccg attcttagcc gtgtggggga tggcacgcag gataacttgt
    20401 cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc cagttcgaag
    20461 tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac ttcagcgcgg
    20521 gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt ctttctccgt
    20581 tgcactcggt ctatgttgac cagtgggact gggaacgcgt aatgggcgac ggtgagcgtc
    20641 aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa gcaaccgaag
    20701 ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc cacttcgtac
    20761 acagccagga gttactgtct cgttatccgg atctcgatgc caaagggcgt gagcgggcga
    20821 tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg agcgatggtc
    20881 atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca gagctgggtc
    20941 atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat gcgtttgagc
    21001 tttcttccat ggggatccgc gtggatgccg acacgttgaa gcatcagttg gcgctgaccg
    21061 gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt gaaatgccgc
    21121 agaccatcgg cggcggtatc ggccagtctc gtttgaccat gctgctgctg caactgccgc
    21181 atatcggcca ggttcagtgt ggtgtatggc cagctgctgt tcgcgagaac gtcccttctc
    21241 tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc cggtatcaaa
    21301 gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg acatcgccac
    21361 ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg tcacgtcgtc
    21421 aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa accattccct
    21481 gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc cgccacgaaa
    21541 ctgctggctt aaaaaacgga ttgcttgttc gatgccttgg gggcctgaaa gctcataacg
    21601 gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttcagcga gagcaatgcg
    21661 catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca ttgagccgga
    21721 agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt tatgtaccac
    21781 cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg tgagcaactg
    21841 tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta gtgtcgccag
    21901 ttctggcggc agaagacgta aaatatcatc gctttgttgc agaccatcaa cctgctcagg
    21961 aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat cgttgcgcgg
    22021 tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca gttccggctg
    22081 ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt taagctgacc
    22141 ggcgctcata tcccacagac gaccagctgc agtattgtta tctgcgagaa tcggttcaag
    22201 ttgtccgctc agcgtcatgc gttcttgaac ttcactcaac agttgttcgc gttcttcttc
    22261 taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc aacgctggag
    22321 aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact gccgggcttg
    22381 ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta gctgcacaat
    22441 aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt cggtgagttc
    22501 cggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc ggggaacatc
    22561 atcagtgatt gccgccttca ggcgtgggaa tttttcaaag aagaccgcca gctgcggtga
    22621 ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg ccagcatcac
    22681 attaagcgta tccagcgtta gcattgttgt gcctggtgaa tctgtgcacc gacatcctgt
    22741 aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt ttgctgttcg
    22801 ctgaataaag cgtgttgctt gcgccagtcg ctttcaagct cttccagttg ttgttttatt
    22861 tcaccaggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc ttgtaaactt
    22921 acatcgcgaa cgacaagatg ttgggcgcta tcaacttcca gattcagttt ctgggcaaag
    22981 ccgataccgt tcagtttacc gcgaatttca ccacctttgc tcagccactg ttccagcgcg
    23041 ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg tttttgcagc
    23101 agtagagtca gagtggaagc agtaacgtta acaggaagtt gatactgctg acgacggctg
    23161 aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg ctgctggagt
    23221 tgcaggtgac gttgcacaat cgcgccaagg cgagtcaaca tcccttgctg ttgccaggca
    23281 tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg cgcgtcatac
    23341 cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg accgctaaaa
    23401 aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga gacataaggc
    23461 gcatccggta atttatccag ttgcaggcgc agcataaaaa tcagctcaaa tacatgatcg
    23521 ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc tgtgacctgc
    23581 aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat ggagcggaaa
    23641 ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc atataacgct
    23701 tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag acgcatcggg
    23761 attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt gagcaaggta
    23821 ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc tgcttccggc
    23881 aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg aatagaaagg
    23941 ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaaa cgcgcgggca
    24001 ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc tggcgggcca
    24061 aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat ggcgtggcta
    24121 cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc cgctaataaa
    24181 tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta aattgcgagt
    24241 atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DAJTVK010000058        23953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000058 DAJTVK010000000
VERSION     DAJTVK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.058"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(38..400)
                     /locus_tag="OO372_002327"
     CDS             complement(38..400)
                     /locus_tag="OO372_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6341802.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="OO372_002328"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="OO372_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6341803.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="OO372_002329"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="OO372_002329"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6341804.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="OO372_002330"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="OO372_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6341805.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /locus_tag="OO372_002331"
     CDS             complement(4827..5036)
                     /locus_tag="OO372_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341806.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="OO372_002332"
     CDS             5321..5683
                     /locus_tag="OO372_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6341807.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6226..6904
                     /locus_tag="OO372_002333"
                     /pseudo
     CDS             6226..6904
                     /locus_tag="OO372_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6948..11798
                     /locus_tag="OO372_002334"
     CDS             6948..11798
                     /locus_tag="OO372_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HCR6341808.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12166..13821
                     /gene="lldP"
                     /locus_tag="OO372_002335"
     CDS             12166..13821
                     /gene="lldP"
                     /locus_tag="OO372_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6341809.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13821..14597
                     /gene="lldR"
                     /locus_tag="OO372_002336"
     CDS             13821..14597
                     /gene="lldR"
                     /locus_tag="OO372_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6341810.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14594..15784
                     /gene="lldD"
                     /locus_tag="OO372_002337"
     CDS             14594..15784
                     /gene="lldD"
                     /locus_tag="OO372_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6341811.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15980..16453
                     /gene="trmL"
                     /locus_tag="OO372_002338"
     CDS             15980..16453
                     /gene="trmL"
                     /locus_tag="OO372_002338"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6341812.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO372_002339"
     CDS             complement(16506..17327)
                     /gene="cysE"
                     /locus_tag="OO372_002339"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6341813.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO372_002340"
     CDS             complement(17407..18426)
                     /gene="gpsA"
                     /locus_tag="OO372_002340"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6341814.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO372_002341"
     CDS             complement(18426..18893)
                     /gene="secB"
                     /locus_tag="OO372_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6341815.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO372_002342"
     CDS             complement(18956..19207)
                     /gene="grxC"
                     /locus_tag="OO372_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6341816.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19349..19780)
                     /locus_tag="OO372_002343"
     CDS             complement(19349..19780)
                     /locus_tag="OO372_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6341817.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20025..21569
                     /gene="gpmM"
                     /locus_tag="OO372_002344"
                     /gene_synonym="pgmI"
     CDS             20025..21569
                     /gene="gpmM"
                     /locus_tag="OO372_002344"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6341818.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21579..22862
                     /gene="envC"
                     /locus_tag="OO372_002345"
     CDS             21579..22862
                     /gene="envC"
                     /locus_tag="OO372_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6341819.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22866..23825
                     /locus_tag="OO372_002346"
     CDS             22866..23825
                     /locus_tag="OO372_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6341820.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6045 a         6108 c         6212 g         5588 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ctttttttcg gatttaattg tgaaatttta
     5941 atcatcatat tcatacaata aattatctgt aaattttttc ccagacatgg tttctttcat
     6001 atttgacatt tattaataat catcattaga atccacgaaa ataatatatt catacaaaca
     6061 cagacatctt ttgaaacgaa tgatacattt ttatgatgaa tgcttaaaac aatatttaat
     6121 cgacacacat cactgtcatt aatgtaaagc aacatagatc accaataata caatggacaa
     6181 ctctaccggc attgatcgct ggtcggagga aataaggatt tttacatgaa cttgaagaag
     6241 atttttttca gtgctgtcac tgtttcagtt ctctgcgcat taacgggatg tgactacatt
     6301 gaagaaggca agccagaaag tagtttattg aaacaacaag aagaacatta aaattgatct
     6361 acttgaaaaa caacaagcac agctaaagag tcaactggaa actattcaaa aacaacaaac
     6421 tggcataata agcagtacaa agactttaac tcacgtaatt aaatccgtta aagatcaaca
     6481 aaacactttt atttttaccg agtttaaccc agcaaaaacg aagtatttca tcctgaacaa
     6541 tggttcggtg gctttagcgg gtcgagtgtt atccattgac gccacagaga atggcagtgt
     6601 tattcatatt tcactggtca acttattaag tacacctatc tcaaatattg gttttaatgc
     6661 gacatggggt ggtgaaaaac ctgtcgatgc caaagagttt gcccggtggc aacaattact
     6721 tttcaacaca tcaatgaaat ccacattgaa attattacca ggtcaatggc aagacattaa
     6781 tttgaccctg aagggtgtat cgcccaataa cctgggatat ctgaagttag ccatcaacat
     6841 ggaaaatatt cagttcgaca atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa
     6901 ataaatgcag ttagtacgct tataacaagg aatagcgagc atcattaatg aacaaaatat
     6961 ttaaagttat ctggaaccct gcgacaggga attatactgt taccagcgaa acggcaaaaa
     7021 gccgtggcaa gaaatctggg cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa
     7081 tgttgtcgtc gtttggggca ttggcgaatg ccgggaatga caacggccag ggtgttgatt
     7141 acggtagtgg atcagctggc gacagctggg ttgctatagg caaaggggcg aaagcaaata
     7201 cttttatgaa caccagtggt tccagtactg ctgtgggtta tgacgctata gctgaaggcc
     7261 aatatagctc tgccatcggg tcaaaaaccc atgcgattgg tggtgcatca atggcctttg
     7321 gggttagtgc aatatcagaa ggcgatagaa gtatagcact gggtgcctct tcgtattcat
     7381 tgggccaata ctcaatggcc ctcggccgtt attcaaaagc attgggtaaa ttgtctattg
     7441 ctatggggga ctcttccaaa gcggaaggag caaacgccat tgccctggga aatgccacta
     7501 aagctactga gattatgagt attgctcttg gcgacaccgc caatgcgtca aaagcgtatt
     7561 caatggcgct gggagcaagt agcgtcgcat ctgaagaaaa cgcaattgcc ctggggcgta
     7621 gcagtgtagc tagcggtact gacagcctcg catttggcag acaatcactt gccagcgcag
     7681 cgaacgctat tgcgataggt gctgagaccg aagccgctga aaatgcaact gctattggca
     7741 ataatgcgaa ggcaaaaggg actaatagca tggcaatggg gttcggaagc cttgccgata
     7801 aagtcaatac tatcgcatta ggaaatggca gccaggctct ggcagataat gcaatcgcca
     7861 taggccaggg caacaaagct gatggcgtgg atgccatcgc tctgggtaat ggtagccagt
     7921 cgagaggctt aaacaccatt gccttaggca cagccagtaa tgcaactggt gataagagtc
     7981 ttgcgcttgg tagtaatagc agtgccaacg gtattaactc tgtcgcgctg ggcgcagatt
     8041 ccattgcgga tttagacaat accgtctctg tcggcaatag ttcattaaaa cgcaagatcg
     8101 ttaatgtgaa aaatggcgcg atcaagtctg acagttacga tgccattaat ggttcacagc
     8161 tttatgccat tagcgactcg gtagcaaaaa ggcttggagg aggggctgca gtagatgttg
     8221 atgacggtac tgttacagca ccaacctaca atttaaaaaa tggtagcaaa aataacgtag
     8281 gggctgcgct cgctgtactt gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat
     8341 acagcgctgc tcatggtact agtagcccaa ctgccagcgt aatcaccgat gttgcggatg
     8401 gcacgatttc agcctccagt aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg
     8461 acgatgtcga agccaacacc gccaatatcg ctactaatac cagcaacatt gccacgaata
     8521 cagcaagtat tgccaccaat accaccaata tcaccaacct gacggattcc gttggtgacc
     8581 ttcaggctga tgccctgctc tggaacgaaa ctaaaaaggc attcagtgca gctcacggcc
     8641 aggataccac cagcaaaatc accaacgtta aagatgccga cctgacggct gacagcactg
     8701 atgctgttaa cggctctcag ctgaaaacca ccaacgatgc tgtggcgacg aataccacca
     8761 atatcgccaa taacacttcc aatattgcca ctaacaccac caacatctct aatctgactg
     8821 agacggtgac taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtattca
     8881 cggcagctca tggcaccgag accaccagca aaatcaccaa cgttaaagat ggcgacctga
     8941 cgactggcag caccgatgcc gttaacggct ctcagctgaa aaccaccaac gatgccgtgg
     9001 cgacgaatac caccaatatc gccactaaca ccaccaacat ctctaatctg actgagacgg
     9061 tgactaatct tggtgaggat gcgctgaaat gggataagga caatggtgtc ttcactgcag
     9121 ctcatggcaa caataccgcc agcaaaatca ccaatatcct ggacggcaca gtcactgcaa
     9181 ccagttccga tgccattaac ggtagccagc tttatgactt aagcagcaat atcgccacct
     9241 acttcggcgg caatgcttct gtgaatactg acggtgtgtt taccggtcca acctacaaaa
     9301 tcggtgaaac aaattattat aacgtcggcg atgcactggc tgcgattaac tcctcattta
     9361 gcacgtctct cggcgatgct ctgctttggg atgccaccgc aggtaaattc agtgccaaac
     9421 acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa atttcagact
     9481 ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat gttgttgatg
     9541 cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg acgtacacca
     9601 ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat accactcttg
     9661 acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc gccgctcacg
     9721 gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc tctgctgcca
     9781 gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc gctgatgcgc
     9841 tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact tacaccattg
     9901 cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat aacgccctgc
     9961 tgtgggatga gactgccaat ggcggtgctg gagcctacaa tgccagccat gacggtaaag
    10021 ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc gatgcagtga
    10081 acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa attatcaatc
    10141 agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt atcaactacg
    10201 ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt gtcggcgcta
    10261 cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt ggtcagaaca
    10321 gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc agccgtgtga
    10381 ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cgtggtgatt ggttatgaca
    10441 ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa taccgccaaa
    10501 tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc cagttgcaga
    10561 acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac tcaacggcag
    10621 aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact gtcgttaatg
    10681 gtaatgcagg tattggtatc ggtttgaaca ctctggttct agctgatgcg atcaacggca
    10741 ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg ggtaacggtt
    10801 ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac gcaccgcaga
    10861 actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc acaaacgtcg
    10921 cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc actgatgagc
    10981 gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact aatctggata
    11041 ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt agcaccaagt
    11101 acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat agcgttgcta
    11161 ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc ggttccgttg
    11221 cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt atcaccaacg
    11281 tagctgccgg tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa tcttctgaag
    11341 caggcggcgt gcgctacgac accaaagctg atggttctat cgactatagc aatatcaccc
    11401 tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc gtcaacaaca
    11461 acgacgcggt gaactacgcg cagttgaagc aaagcgtgca ggaaacgaag caatacaccg
    11521 atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag ttgagcggtg
    11581 gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg ggtgccagca
    11641 tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta ggtgtatcga
    11701 tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat agccagggtg
    11761 aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag ttagtgtgac
    11821 taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata aggcgccccg
    11881 ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac cagacatctc
    11941 cccccacaag aattggccct accaattctt cgcttatctg acctctggtt cacaattccc
    12001 caattaaaac tcacatcaat attgccaata cataacattt agttaaccat tcattgtcat
    12061 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    12121 caacagactc attacacgat gtgcgtggac tccaggagac ctgcaatgaa tctctggcaa
    12181 caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc gcttcccatc
    12241 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    12301 acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    12361 ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg gatcattatt
    12421 gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat cattcgctcg
    12481 tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg tttctgtttc
    12541 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    12601 ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat tgttaacacc
    12661 gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca agtaacaggc
    12721 atcgacagct ttgagattgg ccagatggtg gggcggcagc taccgtttat gaccattatc
    12781 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaagga gacgtggcct
    12841 gcggtcgtgg ttgcgggcgg ctcgtttgcc atcgctcagt accttagctc taacttcatt
    12901 gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct gacgctgttc
    12961 ctcaaacgct ggcagccagt gcgcgtattc cgttttggtg atttgggggc gtcaaagatt
    13021 gatatgacgc tggcccacac cggttacact gcgggtcagg tgttacgtgc ctggacaccg
    13081 ttcctgttcc tgacagctac cgtaacactg tggagcatcc cgccgtttaa agccctgttc
    13141 gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    13201 ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc cgtgtttaag
    13261 tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct ctcgattgtc
    13321 tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct gaaagaactg
    13381 gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc gaactattcc
    13441 ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt caccttcttc
    13501 tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc atctaacgcc
    13561 ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga tctgttgctg
    13621 gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca atctatcgct
    13681 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    13741 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcttatgtc
    13801 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    13861 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    13921 agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actgcgcgag gcgctggcaa
    13981 aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt attcgctggc
    14041 gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg atggccgatg
    14101 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    14161 ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt tgctttgaag
    14221 caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt catctggcga
    14281 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    14341 tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg gttttttcac
    14401 aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat gccgacgggg
    14461 cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa cgattcgatg
    14521 aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat gagcattcga
    14581 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    14641 tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat acacgctgcg
    14701 ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga aaaacatgtc
    14761 cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg tagcactggc
    14821 accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag ccaaagcggc
    14881 ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    14941 cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg atcgcggctt
    15001 tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg ttttcaccgt
    15061 ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga gcggcccaaa
    15121 tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    15181 cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca aaccgaccgg
    15241 actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct catggaaaga
    15301 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    15361 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    15421 tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta ttgcagatgc
    15481 ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc ttgatgtcgt
    15541 gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc tgtatgcgct
    15601 ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa aagagatgaa
    15661 agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag attcgctggt
    15721 gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag ggaatgcggc
    15781 atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga ccgttcggcg
    15841 ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc tcgcccctcc
    15901 ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg ctatcctgcc
    15961 cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa ttccgccaaa
    16021 tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctgcata tcatcgaacc
    16081 gatgggattt gcctgggacg ataagcgcct gcgccgcgcg gggctggact atcacgagtt
    16141 taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa atccccagcg
    16201 cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct atcaggatgg
    16261 cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc ttgatgccct
    16321 gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca tgaatctgtc
    16381 caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc cgggagcgtt
    16441 gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg cacgatcaca
    16501 ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa atgctggtcc
    16561 atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg aacgccagcg
    16621 gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat cttcgcgccg
    16681 cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac accttcacga
    16741 attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac agattgcaga
    16801 atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt ggcgtggtcg
    16861 agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa cgtcacagaa
    16921 acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa ccagtgaccg
    16981 atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt tgagtattta
    17041 tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga ggcgatcatt
    17101 tccgggtcag cggcgtaggc ttcttccacc acttcacgga tagcaatagc aggcataatt
    17161 ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac taccaaggtt ttcgtgcttg
    17221 agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag cgttctggct
    17281 tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc ttactccaca
    17341 cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa caaaggttcc
    17401 ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataacg tcaatgctgc
    17461 ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta ttggcatttc
    17521 tacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc cttccaccac
    17581 ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca tcatgccaaa
    17641 acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca gccccgccat
    17701 gcccataaag gtggcaggat cggcacccag cgccgcgcca agacgcgaca tttcagccag
    17761 cccacgggtg atcagtgccg tacgcgcatt cgcaccaaaa ccgataccgt cggacatccc
    17821 cgcgccaatg gcaatgacgt ttttcaccgc accgccaagc tgcacaccga tgaaatccgg
    17881 gttgctgtaa acgcggaaac ttttgccaca gtgcagcaat tgttggagat catcggcaaa
    17941 ggtctggtcg gtagaggcca acgaaatagc cgtcggtaaa cctgccgcca gttctttcgc
    18001 aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct cacgcgccac
    18061 gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca ccagacgcgc
    18121 atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat ggctgggtac
    18181 gacgacgaga atattacggc tggctgccag cgcagtggcg agatcgcttt caagatggag
    18241 cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc gttcaagcgt
    18301 tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc ttgccagggt
    18361 aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag cattacgttg
    18421 gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg caaatagttc
    18481 atgaacagcg catcgaagtt aatcggcgca aggttcagtt gcgggaatgt accgcgggat
    18541 accacgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca gtatgcgccc
    18601 aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc gccctgctga
    18661 acttcacaca ggaacgcggt ttcttcgccc agggaggccg ttacggtaac acgcagtacc
    18721 acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag tttaacttct
    18781 ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga gatatccttg
    18841 gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga catgtgtaga
    18901 aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc acacgttatt
    18961 tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca cagccgccaa
    19021 tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt ttgatcatct
    19081 cttcacgctt ggcggcattg ccatcgatcg gcaactcctg aaaactcacg cccttgctgc
    19141 tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag atttcaacat
    19201 tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg gcggctgcaa
    19261 aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt gcagctaacg
    19321 gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc aagttttcgc
    19381 cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca gctttcgtca
    19441 gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata accggtttgt
    19501 ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc aacagattga
    19561 tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc acaaccacag
    19621 cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc actttcgagg
    19681 tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag gcgatactca
    19741 gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac aactcccgac
    19801 gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa tacagccagc
    19861 gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa tctggcatcg
    19921 gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga ttcagctgta
    19981 gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg gtttctaaaa
    20041 aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag caggataacg
    20101 ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt ccgcataccc
    20161 tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt aactccgaag
    20221 taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact cgtctggacg
    20281 ttgaaatcaa agatcgcgct ttctttgcta atccagtgct gactggcgcg gtagataaag
    20341 cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc ggcgtacaca
    20401 gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc gcagaaaaaa
    20461 tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct gaatcctcgc
    20521 tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg tccatcattg
    20581 gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa gcctatgacc
    20641 tgctgactct ggcgcagggt gagtttcagg ccgatactgc cgttgctggt ttgcaggcag
    20701 cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt gcagaaggtc
    20761 agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc cgtgctgacc
    20821 gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc gcgcgtaaga
    20881 aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc aaaactgcgg
    20941 ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg aaaaacgaca
    21001 aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc ttcttcaacg
    21061 gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca ccgaaagtgg
    21121 ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa ctggttgcgg
    21181 ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc gacatggtag
    21241 gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat cactgcgtgg
    21301 aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc gctgaccacg
    21361 gtaacgctga acagatgcgc gatccggcaa cgggtcaggc acacacggca cacaccaacc
    21421 tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca ggcggcaaac
    21481 tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg caagagatga
    21541 ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag gcgattaata
    21601 ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc tacgccagcg
    21661 ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag cgtgaccaac
    21721 tcaaatctat tcaggccgat atcgccacaa aagagcgcgc ggtacgccaa aagcaacaac
    21781 aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct gaagccaccc
    21841 gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt gatgagatga
    21901 acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc ctcgccgcac
    21961 aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt ctcagcggtg
    22021 aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac caggcgcgac
    22081 aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt cgccatgcag cgtgctgaac
    22141 tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc caacaggcga
    22201 aactgactca ggctctgaac gagcgtaaaa agacgctggc ggggctggaa tcttccatcc
    22261 agcaaggtca gcagcagcta agtgaactgc gtgccaacga atcccgtctg cgtaacagta
    22321 ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt gaggctcagg
    22381 cagttcgcga caggcagaaa gaagcgacgc gcaaaggcac cacctacaaa ccgaccgaaa
    22441 gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt caggcattct
    22501 ggccagttcg tggccctacg ctgcatcgct atggcgagca gctacagggt gaattacgct
    22561 ggaaaggaat ggttatcggt gcctctgaag gtactgaagt taaagcaatt gccgacggtc
    22621 gggtgattct ggctgactgg ctgcaaggct acggtctggt ggtggtggtt gagcacggta
    22681 aaggcgatat gagtctttac ggctataatc aaagcgcgct ggtgagcgtt ggttcgcagg
    22741 ttcgtgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggc agaccttcac
    22801 tctatttcga aattcgccgc cagggtcagg ccgtcaatcc acagccgtgg ttgggaagat
    22861 aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg gcgctctcct
    22921 cccctgtaat tgctggcaaa cttgccatcg tcattgatga ttttgggtat cgcccgaaca
    22981 acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta cccgattcac
    23041 cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg ttgattcatc
    23101 tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctgcgc ccggagatga
    23161 gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc tatgccgtgg
    23221 ggatcaataa ccacatgggc agcaagatga cctctaacct gtttggtatg cagaaagtga
    23281 tgcaggcgct ggagcgttat aatctttact tcctcgacag cgtaaccatt ggtaataccc
    23341 aggcgatgcg cgccgcgcaa ggcactggcg tgaaggtgat caaacggaag gtgttcctcg
    23401 acgattcaca gaacgaagcg gacatccgtg tgcaattcaa tcgcgcaatt gacctagcgc
    23461 gtcgcaacgg atcaaccatt gccattgggc atcctcaccc ttcaacggta cgcgtgttgc
    23521 aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc agcttgctga
    23581 atgaaccgca ggttgatacg tctacaccgc cgaaaaacac tgtgcccgac acaccgcgta
    23641 atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct gtttatgcta
    23701 atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc gtttacttcc
    23761 agcatcagtg gcaaggctgg ggcaaacagc ctgaagcggc gaagcttaac gctagcgtaa
    23821 attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc attgccataa
    23881 gcggaaagac tacagtaacg cctgataacc cacctttacg ctgcgcacat tggtaatcag
    23941 gcgcttatac ata
//
LOCUS       DAJTVK010000059        23487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000059 DAJTVK010000000
VERSION     DAJTVK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.059"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO372_002347"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="OO372_002347"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6341821.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="OO372_002348"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="OO372_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6341822.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO372_002349"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="OO372_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6341823.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO372_002350"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="OO372_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6341824.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO372_002351"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="OO372_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6341825.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="OO372_002352"
     CDS             5281..5739
                     /locus_tag="OO372_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6341826.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="OO372_002353"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="OO372_002353"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6341827.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="OO372_002354"
     tRNA            6791..6875
                     /locus_tag="OO372_002354"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6923..7054
                     /locus_tag="OO372_002355"
     ncRNA           6923..7054
                     /ncRNA_class="other"
                     /locus_tag="OO372_002355"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OO372_002356"
     CDS             complement(7238..7915)
                     /gene="narI"
                     /locus_tag="OO372_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6341828.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OO372_002357"
     CDS             complement(7915..8625)
                     /gene="narJ"
                     /locus_tag="OO372_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6341829.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OO372_002358"
     CDS             complement(8622..10160)
                     /gene="narH"
                     /locus_tag="OO372_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6341830.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10157..13900)
                     /locus_tag="OO372_002359"
     CDS             complement(10157..13900)
                     /locus_tag="OO372_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6341831.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OO372_002360"
     CDS             complement(14293..15684)
                     /gene="narK"
                     /locus_tag="OO372_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6341832.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16023..17819
                     /gene="narX"
                     /locus_tag="OO372_002361"
     CDS             16023..17819
                     /gene="narX"
                     /locus_tag="OO372_002361"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6341833.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17812..18462
                     /gene="narL"
                     /locus_tag="OO372_002362"
     CDS             17812..18462
                     /gene="narL"
                     /locus_tag="OO372_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6341834.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OO372_002363"
     CDS             complement(18463..19857)
                     /gene="ychO"
                     /locus_tag="OO372_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6341835.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20042..20395
                     /gene="ychN"
                     /locus_tag="OO372_002364"
     CDS             20042..20395
                     /gene="ychN"
                     /locus_tag="OO372_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6341836.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OO372_002365"
     CDS             complement(20439..21134)
                     /gene="chaC"
                     /locus_tag="OO372_002365"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6341837.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OO372_002366"
     CDS             complement(21292..21522)
                     /gene="chaB"
                     /locus_tag="OO372_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6341838.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21792..22892
                     /gene="chaA"
                     /locus_tag="OO372_002367"
     CDS             21792..22892
                     /gene="chaA"
                     /locus_tag="OO372_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6341839.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23295..23402
                     /locus_tag="OO372_002368"
     CDS             23295..23402
                     /locus_tag="OO372_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6341840.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5883 a         6143 c         5665 g         5796 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactactgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 ttagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacggagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacgataa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgcaga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat ggccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggcctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggaa gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcac agcgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct tcccgataag ggagcaggcc agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact tttcaaaagt ccgtaaactc
     6901 tcaaacgaat ccgcaattaa atattctgcc caagcgggga aggatgagaa gcttcgacca
     6961 aggttcgact tgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag
     7021 cgcgcagcgc tgagtaatcc ttcccccacc accatcgctt ccttaaataa tcaaaacctg
     7081 ttactccgag ctaattttgc ccgatggcac cacgtttacc gggcctacaa tatcctcccc
     7141 tgtaggtcgg ataagcgaag cgcatccgac aactcacccc gcaccgtgcc cataaaaaaa
     7201 ccccgccgaa gcagggtcta tgtgaactaa aattcgctta gtgacgagcg cgcaccagct
     7261 ggtacttacg tgtcagatac tccaccggta cgctccagat gtgtaccaga cgcgagaacg
     7321 ggaacagcag gaataacgtc atccccagca ccaggtgcag acggaagata aacgccacac
     7381 catcgaggtg ttgagaagcg ccaccgtgga aggtcaccac cgactgcgcc cagccaacca
     7441 gtttcatcat ctcgctaccg tccatatgct gagcggagaa cggaatggtc aacaggccca
     7501 gcgcgcactg gataacgagc agcgacagga tcaggatatc cgctccggta gtggttgcac
     7561 gcacgcgtgg gctgaacaga cgacgcttca gcaacagcac gccgccaatc agacacagca
     7621 cgccgctggc accaccagca aacatcgcca ttttctgttt cacttcaatc ggcagccagg
     7681 cttcatacat ccagtgcggc gtcagcatac cgaagaagtg accaacaaaa atccccagaa
     7741 taccgatatg gaacaggttc gacgccaggt tcatcccttt gcgatccagc atctggctgg
     7801 acgccgcgcg ccaggtgtac tgcccgtagt cataacgcag ccagctacca atcaggaaga
     7861 ccgccccggc tatgtacggg tagatatcaa agaagaacat attcaggaat tgcattagtg
     7921 ctgtcctccg gtggtgatat tcagatattg cggcgcgacg gcaccggcaa agcgacgctg
     7981 atgagcagtg attgctgaat caccgcagcc tttgtcagca aagaatttaa cctgctcttc
     8041 ttcccagaca gcatccagcg cctgcggcgt atcatcgcgc gcttcgtcgg caattttttc
     8101 cgccactttg tcgctgtcga tagcggtatt ggccaatttc agcagcagat caaacatcac
     8161 ggcataacgg ctttcacgct gttgcagacg cgcgctcagc aatgccagaa tcggcgcgat
     8221 atctttcaaa ccttccacgg cttcgctttg cggcagctgc gacaggtact ccagatacag
     8281 cggcagatgg tccggtaatt cgcggctgtt taactgcaag ccgtgctgct cgtactgcgc
     8341 cagcaggtcc accatcgcct ggccgcggtc acgggattca ccgtgaacat gttcaaatag
     8401 caacagtgac gtggcgcgac cacggtcgaa cagttcgctg tactgggcct gggcatcgag
     8461 cggatccatc gccgttaaat cgcgcaggaa aatgcccagc gcatgggcat cttctttcga
     8521 cagatttttc gacgcagcaa tcgcctcaaa catctcttgt tgatgctgcc ataaggcagc
     8581 atccggatat tcaaggagac gcgatacaat cacgagttcg atcatggatg cggctccgtt
     8641 ttgctggtca catcgatggc atcgatacga cggctgttga acagattgaa tttggtatct
     8701 gaaccgtggc agccatcacc aaaggtaaag ccgcagccat ttttctccgg gaaggcttcc
     8761 cgtgccagtt cacgatgact actcggcacc acaaagcgat cttcgtagtt agcaatcgcc
     8821 agataacggt acatctcctg tgcctgggct tcggtcagac cgacctcttc cagcgcacgg
     8881 gtatcaactt taccgtcaac ggtttcagca cgtttgtaat gacgcatcgc cagcatacgt
     8941 ttcagtgcgc gcagtaccgg tttggtatca ccggcggtca gcagattcgc cagatactgt
     9001 accggaatac gcaagctttc gacgtctggc agaatgccgt tgctacccag ctcacccgcg
     9061 tctgctgcag actgaatcgg agacagaggc ggcacgtacc agaccatcgg cagtgtgcga
     9121 tattctggat gcagcggcag cgccagtttc cattccattg ccattttata aaccggcgac
     9181 tgctgtgcgg cttcaataac gctcagcgga ataccgtctt taatcgcctg ctcgatgact
     9241 ttcggatcgt tcggatcgag gaacacctcc agctgacgct ggtaaagatc tttctcgttc
     9301 tcggtgctgg ctgcacgttc aatcgcgtcg gcatcgtaca gcagcacgcc aagataacgg
     9361 atacgaccga cacaggtttc tgagcacacg gtcggctgac ccgcttcaat acgcggatag
     9421 cagaagatgc acttctcaga cttaccgctc ttccagttga agtagatttt tttgtacggg
     9481 catccggtga tgcacatacg ccagccacgg catttatcct ggtcgatcag gacgatgcca
     9541 tcttcttcac gcttgtaaat cgcaccgctc gggcaggtcg ccacacatgc cgggttcagg
     9601 cagtgttcgc acaggcgcgg caaatacatc ataaaggtgt tttcgaactg gctatacatc
     9661 gccttctgga tgttgtcgaa gttcttgtct ttcgccagtt tgtcaaactc accacccagg
     9721 tcatcttccc agttcggccc tttttcgatt ttcgccatcc gttccccggt aatcagcgaa
     9781 cgcggacggg caatcggctg cgatttgctg ccttccggcg cggtatgcag gttctgatag
     9841 tcaaaatcga acggctcgta ataatcgtcg atccccggca gatgcgggtt agcgaagatt
     9901 ttacccagca acatggcacg gttacccatg cgcggctgca gtttgccgtt gattttacgg
     9961 atccagccgc ctttgtattt ttcctggttt tcccagtcag tcgggaagcc ctgacccggc
    10021 ttggtttcca cgttgttgaa ccacgcgtat tccacgcctt cacggctggt ccagacgttt
    10081 ttacaggtaa ctgaacaggt gtggcagccg atgcacttat cgagattcag caccatgccg
    10141 acttgtgaac gaattttcat tttacgctct cctgtacctg gtcattgcct tcgccatcta
    10201 accagtcaat gttcttcatc ttacgcacta caacaaactc atcgcggtta gaacctacgg
    10261 tgccgtagta gttaaagccg tatgccagat gggcatagcc gccgatcata tgcgtcggtt
    10321 tcggcgtgat acgggtgacc gagttatgga taccaccacg ctgttgggta atttccgaac
    10381 caggcaggtt aacgatacgt tcctgcgcgt ggtacatcat ggtcatccct gccggaacac
    10441 gctggctgac aaccgcacgg gcagtcagag caccgttgct gttgaatact tcaatccagt
    10501 cgttatcggc gatacccaga tctttggcat cggcttcact caaccagacc accggaccac
    10561 cgcgacctaa agtcagcatc agcaggttgt cgctgtaggt ggagtggata ccccacttct
    10621 ggtgcggcgt caggaagttg agcgcttttt ccgggttgcc gttggatttc tggcctatca
    10681 cttctttcac cgaacgggtg tcgatcggcg gacgataaac cagcaggctt tcaccgaaat
    10741 cacgcatcca ctggtgatcc tgatacagtt gctgacgacc agagagcgta cgccatggga
    10801 tcagctcgtg aacgttggtg taaccggcgt tgtaagaaac gtgttcatct tccagaccag
    10861 accaggtcgg gctggagata attttgcgcg gctgtgcctg aatatcgagg aagcggatct
    10921 tctcgtcttc tttattcagc gccagatgcg tatggtcacg accggtaaat tcgctcaggg
    10981 cagcccaggc tttcacggct acctgaccgt tggtttccgg tgccagtgtc aggatcatct
    11041 ctgccgcatc aattgcggtg ttcagcatcg gctggccttt cgccggacct tccgctttgg
    11101 tgtagttgag cttacgcagc agatccatct cgctctgggt gttccaggca atccctttac
    11161 cgccattacc gattttctcc atcaacgggc caatagaggt aaagcgttcg taagtcgccg
    11221 gataatcgcg ctctacgacc ataatgtgtg gcgcggtttt acctgggatc aggtcgcact
    11281 cgcctttttt ccagtctttc acatccagcg gctgcgccag ttcagcggca gagtcatgct
    11341 ggataggcag cgtgacgatg tcggtttctt tacccagatg gccgacgcac acttcggaga
    11401 atttcttcgc gatggctttg tagatttccc agtcgctttt cgcttcccag gccggatcga
    11461 ccgccgcaga cagcgggtga ataaacggat gcatatccga agtattcatg tcgtcttttt
    11521 cgtaccaagt cgccgtcggc aaaatgatgt cggaatagag acaggtgctc gacagacgga
    11581 agtccagcgt aaccaccaga tccagcttgc cttccagacc attgtcctgc cagtccactt
    11641 cttccggctt cacgccgccc tgttgcccca gatctttacc ctggataccg tgctccgtcc
    11701 ccagcaggta cttgagcata aactcatgac ctttaccgga agaaccgagc aggttagaac
    11761 gccagatgaa caggttgcgc gggtggtttt taccgttttc tggttgttct gccgcaaaac
    11821 ggatggaacc ctctttcagg gatttcaccg tatagtccac cggattcatc ccggcttttt
    11881 ccgcttcgcc agcgatagtc agcgggttag tgcctaactg cggtgcagac ggcagccagc
    11941 ccatgcgttc cgcacggacg ttaaagtcga tcaagtgtcc ggtatagcgg gatttgtccg
    12001 ccatcggtga cagcaactct tccgccgtga cggtttcata acgccactgg ctggagtggt
    12061 tatagaaata agaagtgctg ttcatgtgac gcgccggacg ctgccagtca agggcaaacg
    12121 ccagcggctg ccagccggtt tgcggacgca gtttttcctg acctacatag tgcgcccagc
    12181 cgcccccgct ctgaccgaca cagccgcaga aaatcagcat gttgatcaga ccacgatagt
    12241 tcatatcgag gtgataccag tggttcagcc ccgcaccgac gataatcatc gaacgaccgt
    12301 gcgttttatc agcgttatcg gcaaattcac gggcgatgcg aataatctgg ctgcgagaaa
    12361 cgccggtaat ctgctcggcc caggccgggg tataagcttt cacatcgtca tagctggttg
    12421 cacagttaac gtcgttcagg ccacgttcca gaccgtagtt tgccagcgtc agatcataaa
    12481 cggtgctcac cagggcggtg ctgccatcag ccagttgcag gcgtttcacc ggcagtttgt
    12541 gcagcagcac gttttccagt tccactttgt tgaagtgttc cgtgccgtcg ccaccaaagt
    12601 acgggaagcc tacctcagcg atctcatcct ggctacccag caggctcagt tgcagctcgg
    12661 tttcttcgcc agttttgccg tcgcgctgct caagattcca tttgcccttc tcgccccagc
    12721 ggaagccaat agaaccgttc ggcgcaacca tttcgccatt ggtattaaag gcgacagttt
    12781 tccattccgg attgttttcc tggcccagcg catcaaccag atcagcagcg cgcagcatac
    12841 gacctgcagc gtagtaaccg tcgcgttctt ccagcatcac cagcatcggc atgtcggtgt
    12901 agcgacgcac atagtcggtg aaatactggc ttgggttgtc gaggtggaat tcacgcagca
    12961 ttacgtggcc catcgccagc gccattgccg catcggtgcc ctgtttcggt gccagccaca
    13021 gatcgcacag tttggcgatt tcagcgtagt ctggtgtgac ggcaacagtt ttggtgcctt
    13081 tgtaacgcac ttcagtaaag aagtgagcat ccggggtacg cgtctgcggc acgtttgaac
    13141 cccaggcgat gatgtagctg gagttatacc agtcagcaga ttccggtacg tcagtttgct
    13201 cgccccaggt ttgcggagac gcaggaggca ggtcgcagta ccagtcgtag aagcttaagc
    13261 aagtaccgcc aatcagcgag agatagcgtg cacccgatgc gtaagaaacc atcgacattg
    13321 ccggaattgg cgagaaacca gcaacacggt ccgggccgta gtttttgatg gtgtaaacgt
    13381 tagatgcggc gatcagttcg ttcacctcct gccaggaaga acgaacaaat ccaccgcgtc
    13441 cacgcgcctg cttaaagctt ttcgctttat cggcgtcttc aatgatagaa gcccatgcct
    13501 caaccggatc gctatgcagc gccttcgctt cacgccacat tttcatcagg cgtttgcgca
    13561 tcatcgggta tttcaggcgg ttggcactgt aaagatacca ggagtagctg gcaccgcgcg
    13621 ggcagccgcg aggttcatgg tttggcagat ccggacgggt acgcggatag tcagtctgct
    13681 gggtttccca ggtgaccaga ccgtttttga cgtagatttt ccagctgcag gagccggtgc
    13741 agtttacccc gtgggtagag cggacgattt tgtcatgctg ccaacgctgg cgatatccat
    13801 cctcccagtc acggttggta ttgagaagct ggccatgccc atcggcaaag gtttcaccct
    13861 tctgtttgaa gtagcgaaac cggtccagga atttactcat cggttttctc ctgtgggagc
    13921 ctgtcggccc tctgataaat cgacattgct aatgtttaag ggcaaggtaa cgctctgaaa
    13981 cagcgtggga attgataacg atcaagagtg atggggatga aaaataaagt aaattcctaa
    14041 gatacccctt taggataatt aacagccacc acataacaga ctgtaaatta agacgtttta
    14101 tacctgtagt tatttaaact attaagattt ttatgcagat gggtattaag gagtattccc
    14161 catgggtaac atattaatgg ctcttaatga aatccggcga aataagccat ataatatgag
    14221 tatgttgtaa ctaaatgacg cataaaaaaa gcgcgacatc atgccgcgca aaggataatc
    14281 aaatattaag atttattttt tagaatgccg accatatacc gcccaggtaa tcactacgca
    14341 ggcgatatag aaaatcaaaa ataccttcat tgcgccgact ggcgaacccg ttaatgccag
    14401 cgagctacca aacgctttcg ggataaagaa gccaccaata gcgccaatcg cagagatgaa
    14461 acccagcgcc gctgccgtgt cggttgccgc ttcacgcatc gcacgttcgt cagaaccccc
    14521 ttctgctttc acgcgatcca ttgtcagttt acggaagatc actgaaatca tctggaaagt
    14581 ggaaccacta cccagcccag ctgtcaggaa cagcgccagg aagaccgcga agaacgccat
    14641 gaagcttccg ccctgcccgt cagtcggtaa ggtcaggaac agcaggccgc tgaaaatcgc
    14701 catcagaata aagttcacca gcgtgacacg agttccgccc agacggtcag ataatgcacc
    14761 acctgcagaa cgcgccagcg caccaataaa cggcccgaag aaagcgtatt gcagaatctg
    14821 aacatccggg aactgcgttt ttgacagcat cgcaaagccc gcggagaagc cgataaagga
    14881 gccgaaggtt gccagataca gcaggctcat aatccacaga tgaccccgtt tgagtaccgg
    14941 caactgctcc ttgatggagg ctttcgaggt agcaagatcg ttcatgccaa accacgccgc
    15001 aatggtgaag atggcaagga acggcaccca tatccaggac gcattcgcca gatacagctc
    15061 agtcccatcc ggctgtttga cgccctggct accaaatact gcgaaaatcg acagtgatac
    15121 caccagcgga gcaaccaact gcatgacgct gacgcccatg tttcccagac caccattcag
    15181 acccagcgcg ccaccctgct tctgtttcgg aaagaagaag ctgatgtttg ccatactgga
    15241 tgcgaagttc gcgccagcaa agccgcatag cagagagatg atgatgaaga cgctataagg
    15301 cgtggaggta tcctgcacgg caaaacccag ccagacgcaa ggaataatca gaataccggt
    15361 gctgaacgcc gtccagcgac gaccaccgaa gataggaacc ataaaggagt atggaacacg
    15421 taataacgcg ccagaaaccg aaggcagcgc agtcaacata aatagctgat cggtcgtaaa
    15481 attaaaaccg actttcggta agttcaccgc aacagcgctg aacaacatcc atacgcaaaa
    15541 cgccagcagc agacagggaa cggaaatcca caggttacgg ctggcaatac gttgaccgcg
    15601 ttgttgccag aacgcaggat cttccggtcg ccaatctgta atgacagctc cagtagccct
    15661 ttcgggggcg gatgagtgac tcatagacac ctctgatact cgtttcgatg tctgccacct
    15721 tagtgtctgt agctaaaggc aatttgatgt aaatcaaacg ataagataac tttatctatc
    15781 attgatattt atcattaccc atagtgagta cagtgatttc ataaaaatta tgagattttt
    15841 cacggtgctg taaaatccct acccttaccg atgtaaagcg actaaccaca cggcaaataa
    15901 ggagtaactc tttccgggta tgggtatact tcagccaata gccgagaata ctgccattcc
    15961 agaatgtatc gtcacattca ttaaggttat tgctcattta aagcctgaag gaagaggttt
    16021 gcatgcttaa acgttgtctc tctccgctca ccctggttaa tcaggttgcg cttattgtgt
    16081 tgctttctac tgctattgga ctggcaggga tggcggtttc tggctggctg gtgcaaggcg
    16141 ttcagggcag cgcccatgcg atcaacaaag cgggatcgct gcgcatgcaa agttaccgtc
    16201 tgttggcggc agtgccatta agtgagaaag acaagccctt aattaaagag atggaacaaa
    16261 cggcatttag cgccgagtta actcgagcag cagaacgaga cgaacaactg gcgcaattac
    16321 agggtttaca agattactgg cgtaatgaac tgatccctgc actgatgcgt gcacaaaacc
    16381 gcgaaacggt gtcagcggat gtcagccagt ttgttgccgg gcttgatcag ctggtatctg
    16441 gttttgaccg caccacggaa atgcgcattg agacagtggt actggtccat cgggtaatgg
    16501 cggtatttat ggcactttta ctggtgttca ctattatctg gttgcgggcg cgactgctac
    16561 aaccgtggcg gcaactgctg gcaatggcga gtgccgtcag tcatcgcgat tttacccaac
    16621 gcgcgaacat cagcgggcgc aacgaaatgg cgatgcttgg aactgcattg aacaatatgt
    16681 ctgcagaact ggccgaaagt tatgccgtac ttgagcagcg ggttcaggag aaaaccgccg
    16741 ggctggagca taaaaatcag atcctctctt ttttatggca ggctaaccgc cgtttgcatt
    16801 cccgcgcccc gctgtgtgaa cgcctgtcac ctgtactcaa cggcttacag aatttaaccc
    16861 tgctacgtga tatcgaattg cgggtgtatg acactgatga tgaagagaat catcaggagt
    16921 ttacctgcca gccagatatg acttgtgatg ataaaggctg ccagctctgc ccgcgcggcg
    16981 tattacccgt tggtgatcgc ggcacgaccc tgaagtggcg gctggctgac tctcatacgc
    17041 agtacggtat tttgctggcg accctgccgc aggggcgtca tcttagccat gatcaacaac
    17101 aactgctgga taccctggtt gaacaactca ccgccacgct ggcgctggat cgccatcagg
    17161 aacgtcagca acagttgatc gtgatggaag agcgtgccac cattgcgcgc gaactgcatg
    17221 attctattgc ccaatctctc tcttgcatga agatgcaggt gagttgttta cagatgcagg
    17281 gcgatgcgct gccagaaagc agccgcgaac tgttaagtca gatccgtaac gaactgaatg
    17341 catcctgggc gcagttgcgt gaattgctca ccacattccg cttgcagctc accgagcctg
    17401 gattacgtcc ggcgctggag gcgagttgcg aagagtacag cgccaaattt ggcttcccgg
    17461 tgaagctgga ttatcaattg ccgcctcgcc tggtgccttc gcatcaggca atccacttgt
    17521 tgcaaattgc ccgtgaggca ttaagtaacg ccctcaaaca ttcgcaagcg agtgaagtcg
    17581 tggtgacggt ggcgcaaaac gataatcagg tcaaactgac cgtccaggat aacggctgcg
    17641 gcgtgcctga aaatgccatc cgcagcaatc actacggcat gataataatg cgcgaccgtg
    17701 cgcaaagttt acgaggcgat tgccgcgtcc gccgtcgtga atcaggtggc accgaagtgg
    17761 tggtcacctt tattcccgaa aaaactttca cagacgtcca aggagatacc catgagtaat
    17821 caggaaccgg ctactatcct gctgattgac gatcacccga tgctgcgaac tggcgtaaaa
    17881 cagcttatca gtatggcacc agatatcacc gtggttggcg aagcgagtaa tggcgaacag
    17941 ggtattgaac tggcggaatc tcttgatccc gatctgatcc tgttagatct caatatgccc
    18001 ggcatgaacg gtctggaaac gctggataaa ctgcgcgaaa agtccctctc agggcgcatt
    18061 gtggtattca gcgtctctaa ccatgaagaa gatgtggtca ccgcactgaa acgcggcgcg
    18121 gatggttatc tgttaaaaga tatggaaccg gaagatctgc tgaaagcatt gcatcaggca
    18181 gctgctggcg aaatggtatt aagcgaagca ttaacgcctg ttctggccgc cagcttgcgc
    18241 gctaaccgtg ccactactga gcgcgatgtt aaccagttaa ccccacgcga gcgcgatatt
    18301 ctcaagctga ttgcccaggg tttgccgaac aagatgattg cccgccgcct ggatatcacc
    18361 gaaagcacag taaaagtgca cgtcaagcac atgctgaaga aaatgaagct caagtctcgc
    18421 gtggaagcag cggtatgggt gcatcaggag cgcattttct gattacggtt cccagcgcag
    18481 ttcgtcgttt gacaatgcat cgaacggttc gacaagcgtt agcgtgatct cattggagga
    18541 gacacgctgc ccctggttat cttccaccac caccgacaat cgccagtgat tgctcgcccc
    18601 ttccccgttc tgccagtcag gcatgatcag cgtccagccc tccgcgctgt tggcttgtgc
    18661 gcccggcgtc aaacttaata tctgcgtatc gccctgccaa atcagttgcc gaataccgta
    18721 acgactgcgg atttgtaatt tcagcggcac tgtttcgcca ggttttagat cccacggcgg
    18781 tgtcgccaga aacaccgtta acgtttttcg ctgtcggtac tcaagagtcg gtagattatt
    18841 tcgctgcgga ttatcatagc gactaccacg taacgactga ctttcggcaa cctcgcccgc
    18901 agaaagttgt tttttgagcg gcacaccaaa gcggtaatta agattcagtc cgaggttatt
    18961 ttgattttcg ccactttcac cctgtttatg ctgggccgtc acagtgacta atggcacagg
    19021 ggtgtaattt aatcccagac tcaacgcgac gggattgtga taacccgtac cagagttaaa
    19081 caaatcaaca cgctcaccaa aatactgttc taccctgaca ctggtattga ggtgttgata
    19141 gaacggcatg cgcatccggg ctgtcaggtc atacccgcgc gccatccgtt gttcctgcgt
    19201 ggctgtctgt tcatgccatg cagcaaacgg ctgataaaag tttgccgata gtcgcaaata
    19261 ttcgccccac gcttcggcac caaagcccgc tcgctgaaga ttttcgtcca gcaagttgtc
    19321 ataaaaagtg ttataaccca ccagccagtt gccgcgcgcc cagcgttgcc caacgcccac
    19381 attgctcacc aacccatcat cctgctgagt aagaccaagc tggctccagg tgagataacg
    19441 atcattatct tgtaacggca caaaccagct tccacgactg ccggtgaaat gtccttcgtt
    19501 atccactttg acgtcaacac tggcatttcc ccacggtgat agccaggact ctacgtgctg
    19561 attaacctgt tgactaagcg cgtcgcggac ttttcccaat gcgaaagctt ttgcctgctc
    19621 gccagtatcc agcccgttat cattcatact ggtttcgccg aaatctttca caatttcagc
    19681 aaagtgtttt tccccatcat ggttttcggg agccatgcct aaatccggca gaccatcatt
    19741 gttattatca aagggatttg ccgctttttg ctcgaaggta gattgtgcgt tagctgtacc
    19801 gcctgccgcc agcaagagta aataaaacgg gataatgcga ggaacgaaac ggctcaaaac
    19861 gtcggtaact tccggtaata atgaaaaacc atagccttaa agaataacga ttactggcaa
    19921 cttttgtcgt attttctcgc gctttcagga aaatccttaa aaaggtgacc gattggtcag
    19981 acgctgcaga agcagtaaga tagaagatga gtgtctaatt ccctgaatac aggagtaaat
    20041 aatgcaaaaa atcgtgatcg ttgccaatgg cgcaccttac gggagcgaat ccttgtttaa
    20101 cagcttgcgg ctggccattg cgttacgaga gcaggagagc aatctggatc tgcgtctgtt
    20161 cctgatgtct gatgcggtca cagccgggtt gcgcgggcaa aaaccagggg aaggctacaa
    20221 cattcagcaa atgctggaga tccttaccgc tcagaatgta ccggtgaaat tgtgcaaaac
    20281 ctgtaccgac gggcgcggga ttagcacact tcctcttatt gatggggtgg aaatcggtac
    20341 tcttgtggaa ctggcgcaat ggacgctgtc agccgataaa gtgctcacat tttaatgttt
    20401 gagggggcgt ctgacgcccc actatgaggg aagtttactc aggcgaatcc cggccgtaac
    20461 acaccatccg gataattctc cgccagcagt ttttttaccg ataccagcaa atcattcagc
    20521 ccatcgtcct gcattcccag tttgataagc tcctgttcca gtgaaaacag gtattgtgcg
    20581 ttggttccca gcggaccgct cgccgccgca atcaacggcg cgatgacctg agcgcgagtg
    20641 tcagattcat attctggatg tcgcgggtcc ataataaaca caatggcgtt tactgtgcgt
    20701 ccatcatcaa gatcaagctg acaccaggtt ggcagataac agccggtaat catctcgcgc
    20761 ttccacaaca gggttagttc ctgctccagc gtctcttctg gtagtcgata ggcgacgcct
    20821 gtggtgcgtc cgccctcttt cagtgcaagc atccgtcccg gctggtgcgc agtcccgcgc
    20881 ccggcggtca ggcgcaggca aaacgcgcga tgccatccaa ccagtgtacc ggtgcataat
    20941 tcggtaaact ccagtgccgg attccacatc aacgatccat aaccgaagat ccacaccggt
    21001 ccctcattag gtcgacaagc cagcgtcgcc gccagcgaag ccgcccgttg ttctgctgac
    21061 cataagagtg attcttcaat ggcaccaaat gccgttttac aatcggcatt catcaagaaa
    21121 tcacgcgtta tcaccttaca cctccgccac tgcatacttc cctgcgacaa ctttttggtg
    21181 tcttccggac atttttaccg ctcattttga aaccactaac gggacaatat gcaatccacg
    21241 gaccacttgc aaggcaacac cgcacgaata gctttaacta agctaccggt tttacgattt
    21301 tttatgccat ttatcatcat cccctttggc atattcatgc ttcacagcag cccaggccac
    21361 tttatgcgcg gtttcttcgc gactggcgtc atcacgccga tcttctttat ctttatattg
    21421 atcccatgcg ctgttgaacg cttctttata gatatcctgg gcatgagacg gtagaacgtg
    21481 ctttacgctt tccggcagat cgcttttcgt tttatacggc atcgttgact ccttttacaa
    21541 agttgaacat taagtgtggt caacaatatc tactcttgcc aaaacataag aattttcatg
    21601 tcagactgtt cttaagaaac gctaaaacaa acattgtcta aacgtaatac ttatttttat
    21661 gccccaatga gtaaaaaatc gacagcgggg taaaaattcg taaaatgtta ccgatatcca
    21721 gacactttct gttatttttc ctacctattt tttacatttt cgataaattc atctgcaaag
    21781 ggataaccat aatgtcaaat gctcaagagg cggtaaaaac ccgccacaag gagacttcgc
    21841 ttattttccc ggttctggcg ctggtagtgc tgttcctgtg gggaagcagc cagacactac
    21901 cagtggtcat tgccatcaat cttcttgcgc ttattggtat tttaagtagc gcctttagtg
    21961 ttgtccgtca tgcggacgta ttagcccatc gcctgggaga accttacggt tcgcttattc
    22021 ttagcctttc agtggttatt cttgaagtca gtttgatttc agctttaatg gcaaccggcg
    22081 acgccgcgcc aacgctaatg cgtgatacgc tctattcaat cattatgatt gttaccggtg
    22141 ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt tgccacccaa tatatgaatc
    22201 tgtttggtat caagcagtat ttaattgcgc tgttccccct ggcgataatc gtactggtat
    22261 ttccaatggc tctgcctgcg gcgaattttt caaccggtca ggcgttactg gtagcattaa
    22321 tttctgcggc aatgtatggc gtatttttgc tgatccagac caaaacgcat caaagtttgt
    22381 ttgtctacga gcacgaagat gacagtgatg acgacgaccc gcaccacggt aaaccgtctg
    22441 cccatagcag cgtgtggcat gctatctggt tgattatcca tctgattgcc gttattgcag
    22501 tcaccaaaat gaatgccagc ccgctggaga cattgctcga cagcatgaat gcccctgtcg
    22561 cctttactgg cttcctggtg gctctgttga ttctgtcgcc ggaaggttta ggtgcgttaa
    22621 aagcagtgtt gaacaaccag gtccagcgcg caatgaatct gttctttggt tcagtgttag
    22681 caaccatttc gcttaccgta cctgtcgtca cgctaattgc ctttatgacg ggtaacgaat
    22741 tacagtttgc acttggtgcg ccagaaatgg tggtgatggt ggcctcttta gtgctgtgcc
    22801 atatctcctt ctccaccgga cgtactaacg tgctcaatgg cgcagcgcat ctggcactgt
    22861 ttgccgccta tttgatgacg atatttgcct gacagttata tctcctccga tgacggaaat
    22921 cgggggagat aagataaatt cctgcttatt gtatttcttc atactacctg gacagctcgt
    22981 taatcagaat tgcgagcctg taccacaacc tgcacgaaac cactgcaaca gctgttgaaa
    23041 agccggaatg tactctttat tgcaaacatt tcagggatga agtgaagatg aattgagacg
    23101 tagtatccac accaagccgg aattatccgg tgaggcgcaa tgttgcgggg gctttatccc
    23161 tggtggcatt ggttgctgga aagagaaaac ccccgcacgt tgagaggtaa aacctgacaa
    23221 caaaacgggg gctaatcttg aaaccagaca cttcaagaat agccgccttg ttgtcattac
    23281 aacaaggcgg ctatatgacg ctcgcgcagt ttgccatgac tttctggcac gacctggcag
    23341 caccgatcct ggcggggatt attaccgcag cgattgtcag ctggtggcgt aaccggaagt
    23401 aaggtgaaaa cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg
    23461 ggtaacagcc tgcggccatt tttgtct
//
LOCUS       DAJTVK010000060        23237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000060 DAJTVK010000000
VERSION     DAJTVK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.060"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="OO372_002369"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="OO372_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR6341841.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="OO372_002370"
     CDS             509..1138
                     /locus_tag="OO372_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCR6341842.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="OO372_002371"
     CDS             1165..2826
                     /locus_tag="OO372_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR6341843.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="OO372_002372"
     CDS             3065..3124
                     /locus_tag="OO372_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6341844.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="OO372_002373"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="OO372_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR6341845.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OO372_002374"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="OO372_002374"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6341846.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OO372_002375"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="OO372_002375"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6341847.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OO372_002376"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="OO372_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6341848.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="OO372_002377"
     CDS             9507..10127
                     /locus_tag="OO372_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6341849.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="OO372_002378"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="OO372_002378"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6341850.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OO372_002379"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="OO372_002379"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6341851.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OO372_002380"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="OO372_002380"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6341852.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="OO372_002381"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="OO372_002381"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6341853.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OO372_002382"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="OO372_002382"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="OO372_002383"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="OO372_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR6341854.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="OO372_002384"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="OO372_002384"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6341855.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="OO372_002385"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="OO372_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6341856.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OO372_002386"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="OO372_002386"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6341857.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="OO372_002387"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="OO372_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6341858.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="OO372_002388"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="OO372_002388"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6341859.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="OO372_002389"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="OO372_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6341860.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5653 a         6332 c         5906 g         5346 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcaccctgtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgcctgt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttacgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc ctcgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAJTVK010000061        23157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000061 DAJTVK010000000
VERSION     DAJTVK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.061"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO372_002390"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO372_002390"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6341861.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO372_002391"
     CDS             complement(1672..2730)
                     /locus_tag="OO372_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6341862.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO372_002392"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO372_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO372_002393"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO372_002393"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6341863.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO372_002394"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO372_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6341864.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO372_002395"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO372_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6341865.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO372_002396"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO372_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6341866.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO372_002397"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO372_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6341867.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO372_002398"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO372_002398"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6341868.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO372_002399"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO372_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6341869.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO372_002400"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO372_002400"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6341870.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO372_002401"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO372_002401"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6341871.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO372_002402"
     CDS             complement(17699..18322)
                     /locus_tag="OO372_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6341872.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="OO372_002403"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="OO372_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCR6341873.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO372_002404"
     CDS             complement(18971..19213)
                     /locus_tag="OO372_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6341874.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO372_002405"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO372_002405"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6341875.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="OO372_002406"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="OO372_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6341876.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="OO372_002407"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="OO372_002407"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6341877.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..>23157
                     /gene="ypdA"
                     /locus_tag="OO372_002408"
     CDS             22593..>23157
                     /gene="ypdA"
                     /locus_tag="OO372_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCR6341878.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         6485 a         5420 c         5044 g         6208 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattt tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgctg ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caagagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gtttatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatccatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcagtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgc
//
LOCUS       DAJTVK010000062        22703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000062 DAJTVK010000000
VERSION     DAJTVK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.062"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="OO372_002409"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="OO372_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6341879.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="OO372_002410"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="OO372_002410"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6341880.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="OO372_002411"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="OO372_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6341881.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="OO372_002412"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="OO372_002412"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6341882.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="OO372_002413"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="OO372_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6341883.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="OO372_002414"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="OO372_002414"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR6341884.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OO372_002415"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="OO372_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6341885.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8811..8975)
                     /locus_tag="OO372_002416"
     CDS             complement(8811..8975)
                     /locus_tag="OO372_002416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341886.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="OO372_002417"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="OO372_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6341887.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="OO372_002418"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="OO372_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6341888.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="OO372_002419"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="OO372_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6341889.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="OO372_002420"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="OO372_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6341890.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="OO372_002421"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="OO372_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6341891.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="OO372_002422"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="OO372_002422"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6341892.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="OO372_002423"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="OO372_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6341893.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="OO372_002424"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="OO372_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6341894.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="OO372_002425"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="OO372_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6341895.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="OO372_002426"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="OO372_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6341896.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="OO372_002427"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="OO372_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6341897.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="OO372_002428"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="OO372_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6341898.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="OO372_002429"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="OO372_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6341899.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="OO372_002430"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="OO372_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6341900.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="OO372_002431"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="OO372_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6341901.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OO372_002432"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="OO372_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6341902.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..22596
                     /locus_tag="OO372_002433"
                     /pseudo
     CDS             22225..22596
                     /locus_tag="OO372_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         5088 a         5690 c         6450 g         5475 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc tgcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgcgtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtttg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcatcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccata gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat ggcgctagac tggccccctg aatctccaga
    22621 caaccagtat cacttattta agtgatagtc ttaatactag tttttagact agtcattgga
    22681 gagcagatga ttgatgtctt agg
//
LOCUS       DAJTVK010000063        22239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000063 DAJTVK010000000
VERSION     DAJTVK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.063"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            75..1463
                     /locus_tag="OO372_002434"
     CDS             75..1463
                     /locus_tag="OO372_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6341903.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRLQK
                     LVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDE
                     FCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKENDTLHDAYKASDER
                     LYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="OO372_002435"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="OO372_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR6341904.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /locus_tag="OO372_002436"
     CDS             complement(2010..2114)
                     /locus_tag="OO372_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341905.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="OO372_002437"
     CDS             2284..2730
                     /locus_tag="OO372_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR6341906.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OO372_002438"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="OO372_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR6341907.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="OO372_002439"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="OO372_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR6341908.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="OO372_002440"
     CDS             4841..5188
                     /locus_tag="OO372_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR6341909.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OO372_002441"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="OO372_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR6341910.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="OO372_002442"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="OO372_002442"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR6341911.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="OO372_002443"
     CDS             6705..6803
                     /locus_tag="OO372_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR6341912.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="OO372_002444"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="OO372_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR6341913.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="OO372_002445"
     CDS             complement(6939..7187)
                     /locus_tag="OO372_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6341914.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="OO372_002446"
     CDS             complement(7335..7517)
                     /locus_tag="OO372_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR6341915.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OO372_002447"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="OO372_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCR6341916.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OO372_002448"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="OO372_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCR6341917.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OO372_002449"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="OO372_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR6341918.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="OO372_002450"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="OO372_002450"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR6341919.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="OO372_002451"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="OO372_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR6341920.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="OO372_002452"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="OO372_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR6341921.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="OO372_002453"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="OO372_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6341922.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OO372_002454"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="OO372_002454"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6341923.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OO372_002455"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="OO372_002455"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6341924.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OO372_002456"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="OO372_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6341925.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OO372_002457"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="OO372_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6341926.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21392)
                     /locus_tag="OO372_002458"
     CDS             complement(19482..21392)
                     /locus_tag="OO372_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6341927.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21524..21868
                     /locus_tag="OO372_002459"
     CDS             21524..21868
                     /locus_tag="OO372_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6341928.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5445 a         5698 c         5403 g         5693 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc ataaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacact atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cccccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aggaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattggt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattcg
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcaaggaaac gaaccgcacg ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg
    19561 agaaacgtcg cgccgtaagg acgcatcacc agccgattgt cacaaattac cagcacgccg
    19621 cgatcgtcgg cgtcgcgaat cagtcgccct actccctgct tgagagtaat gaccgcatcc
    19681 ggcagctgca cttcatcgaa cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct
    19741 tttaacagta gatcatccgg cgaggtaaac ggcaatttgt cgataattac caatgacaat
    19801 gtatcgccac gcacgtccac cccttcccag aaactactgg ttgccacaag aagcgcgtta
    19861 ccggcgctga caaattgctg caacagttgc cctttgctgg tttccccctg caacaaaacg
    19921 ggaagcgtca tggtagcgcg gaactgctcg gccagatcgc gcatcatggc atgcgaggta
    19981 caaagcataa aacaacgacc gttgttagct tcgatgatcg gtcgcagcat tgccgccagt
    20041 tgccgtgcac aacccggttg attggtttgc ggcaggttgc gcggcacaca gagtaacgcc
    20101 tggcggctgt aatcaaacgg gcttggcaat agcaacgact ccgcctgttc tatcccaagc
    20161 cgcgaggtga aatgatgcag atcgtcgttc accgacagcg ttgctgaggt gaagatccag
    20221 ctaccgggct tttgcgccat taactctttg aatttatccg ccacgctgag cggcgtgaga
    20281 gccagagtaa aatggcgcga agtgcattcg taccagtagc tgtagcccgg ctgattgatc
    20341 tcttttagcc gcttcagccg tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc
    20401 aaggcggaac gccccagtga cagtttcgcc acgtcataac aaagttccag ggtgtcatcg
    20461 agcagtaaaa atgcccgctg aatttgcgga ttagctaaca gctcacgcag gttaccgcga
    20521 taacctggct caccgagttg cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg
    20581 cacttttgta actgctgggt gtcttttaat tcggtgcggt aggcgatggt gatgtctttt
    20641 gccaggtcga gcagttgtcg actggagagt gactgaccaa aatactggct ggcaatgtcc
    20701 ggtagctggt gggcttcgtc gaagatcatg acgtccgctt cagggatcaa ctcgccaaat
    20761 ccactctctt taaccaccat atccgccaga aagagatgat ggtttaccac caccacatcg
    20821 gcgtccatcg cttttttacg tgctttgacc acaaagcaat ctttatacat cgggcagtcg
    20881 ctgccaagac agttgtcgtt ggtgctggtg accagcggcc acgcctgtga atcttccgcc
    20941 acgctgacgc aggtgctgat atcaccatcg actgtttgat tagaccagga gcgcagcagg
    21001 atcacatcgc ttaagatttg taccggcaga tcgccccccg ccagcgcctg ctgttcgaga
    21061 cgttcgaggc agaggtagtt tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc
    21121 aatgcctttg agactgttgg caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag
    21181 ccggtcgaga taatgacttt ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt
    21241 ttgcccgtac cggttcctgc ttccaccacc agcggctggc ctttttctat cgcctgggtg
    21301 acggctaccg ccatctgtcg ctgtggttct cgcggcttaa agcctggtat cgctttcgcc
    21361 agctgaccgt ctggtgcaaa atcgtccgtc acactacccc ctgttgattt gaacagggat
    21421 tatgtcagga tgagggcgct ttcgccagtt gaagtggtga cggcaacctc acattgtggc
    21481 agtctttgca gcacgaaaat ggaagtataa tgaggacaaa atgatgacta tcgttcgtat
    21541 cgatgctgaa gcccgctggt ctgatgtagt aatccacaac aacacgctct actacactgg
    21601 tgtaccggaa aacctcgacg ccgatgcctt tgagcaaacc gccaacacgc tggcacagat
    21661 tgacgccgtg ctggaaaaac agggcagcaa taaatcgagc attctggatg ccaccatttt
    21721 cctggccgat aaaaacgact tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc
    21781 gggtcatgcg ccagtgcgct gcacggtaca agcgggtttg atgaacccga agtataaagt
    21841 tgaaattaag attgtggctg cggtgtaagc tttatcgaag caaaataagt cagacgataa
    21901 tttatcgata atactggtcg gttttacata aatcgaccag agaataagat attactgttg
    21961 atgcttctgc taattcatta tttatattta taatttcaat tttatctata aaactaacta
    22021 tataaataaa cataaccatg ccgttgtgtt cgccctttga ttagcaactc tggttttctt
    22081 tcttagctga acaatctggt tatagcgtgc ggactgaccg ttaattttct ccttcttact
    22141 ggataacaca ccatgagctg ctacctttaa aacacctgaa tgtcaacagg ttaactcgtg
    22201 ccatatcgtt ataatccctt tgatattaga tgcaaatta
//
LOCUS       DAJTVK010000064        22132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000064 DAJTVK010000000
VERSION     DAJTVK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.064"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..525)
                     /locus_tag="OO372_002460"
     CDS             complement(<1..525)
                     /locus_tag="OO372_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6341929.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
     gene            821..1597
                     /gene="exuR"
                     /locus_tag="OO372_002461"
     CDS             821..1597
                     /gene="exuR"
                     /locus_tag="OO372_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR6341930.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1942..2604
                     /locus_tag="OO372_002462"
     CDS             1942..2604
                     /locus_tag="OO372_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6341931.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            2608..2991
                     /gene="mzrA"
                     /locus_tag="OO372_002463"
     CDS             2608..2991
                     /gene="mzrA"
                     /locus_tag="OO372_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6341932.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3138..3506
                     /locus_tag="OO372_002464"
     CDS             3138..3506
                     /locus_tag="OO372_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR6341933.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            3544..3849
                     /locus_tag="OO372_002465"
     CDS             3544..3849
                     /locus_tag="OO372_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6341934.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3852..4256
                     /locus_tag="OO372_002466"
     CDS             3852..4256
                     /locus_tag="OO372_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6341935.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4246..4545
                     /locus_tag="OO372_002467"
     CDS             4246..4545
                     /locus_tag="OO372_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6341936.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4731..5123
                     /locus_tag="OO372_002468"
     CDS             4731..5123
                     /locus_tag="OO372_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6341937.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5193..6206
                     /gene="yqjG"
                     /locus_tag="OO372_002469"
     CDS             5193..6206
                     /gene="yqjG"
                     /locus_tag="OO372_002469"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6341938.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            6471..6836
                     /locus_tag="OO372_002470"
     CDS             6471..6836
                     /locus_tag="OO372_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6341939.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7078..7434
                     /locus_tag="OO372_002471"
     CDS             7078..7434
                     /locus_tag="OO372_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6341940.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OO372_002472"
                     /pseudo
     CDS             complement(7485..8381)
                     /gene="yhaJ"
                     /locus_tag="OO372_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            8486..9187
                     /locus_tag="OO372_002473"
     CDS             8486..9187
                     /locus_tag="OO372_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6341941.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9210..9374
                     /locus_tag="OO372_002474"
     CDS             9210..9374
                     /locus_tag="OO372_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341942.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(9508..10818)
                     /locus_tag="OO372_002475"
     CDS             complement(9508..10818)
                     /locus_tag="OO372_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6341943.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10846..12177)
                     /locus_tag="OO372_002476"
     CDS             complement(10846..12177)
                     /locus_tag="OO372_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6341944.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OO372_002477"
     CDS             complement(12452..13816)
                     /gene="tdcG"
                     /locus_tag="OO372_002477"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6341945.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13888..14277)
                     /locus_tag="OO372_002478"
     CDS             complement(13888..14277)
                     /locus_tag="OO372_002478"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6341946.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OO372_002479"
     CDS             complement(14291..16585)
                     /gene="tdcE"
                     /locus_tag="OO372_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6341947.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OO372_002480"
     CDS             complement(16619..17827)
                     /gene="tdcD"
                     /locus_tag="OO372_002480"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6341948.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OO372_002481"
     CDS             complement(17853..19184)
                     /gene="tdcC"
                     /locus_tag="OO372_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6341949.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OO372_002482"
     CDS             complement(19206..20195)
                     /gene="tdcB"
                     /locus_tag="OO372_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6341950.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OO372_002483"
     CDS             complement(20294..21232)
                     /gene="tdcA"
                     /locus_tag="OO372_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6341951.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            21421..21765
                     /gene="tdcR"
                     /locus_tag="OO372_002484"
     CDS             21421..21765
                     /gene="tdcR"
                     /locus_tag="OO372_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6341952.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         5801 a         5649 c         5169 g         5513 t
ORIGIN      
        1 gcgttgattc cctgcattaa ctaatttttg ggcagcattt acttctaact tagcaatagg
       61 ttgctgaggt ggaacactaa caacaatccc ccaaattaag ttaactgtta actgcataga
      121 gagatctttt ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg
      181 ataatttttt tctttttgct ctggttccat tgcaacaaag cgcacagttt tgctactacc
      241 ggctggtaaa gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat
      301 ctcaacttca ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat
      361 gtactgaact tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat
      421 ggaaacagcc ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa
      481 taaaagcggc gtaagccgtg ataagagatt aattttcgcc accatataaa tttgatctta
      541 caatattgtt tccaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa
      601 aaaagcattt ttagttaata ttaaatttaa aggctgggaa ttaacttact gaaatttagt
      661 agctttcaca atgacgatac ttatttttta ggaatttata aatattcaca atattaattc
      721 aatacattaa ttcaatcacc aaatttatat atgaccaatg gcatacccat gaataaagtg
      781 gtatgataac ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg
      841 ccgtttgtat caacaacttg ccgctgacct gaaagagcgc attgaacagg gcgtctatct
      901 ggtgggtgat aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac
      961 ggtagttcgt gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg
     1021 ttcaggtatt catgtggttt ccaaccagcc gcgccatcag caggcggctg acaataatat
     1081 ggaattcgcc aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa
     1141 tattgccgag ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat
     1201 tcaggaacag gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca
     1261 cattcaggta gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg
     1321 gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac
     1381 cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc
     1441 ccatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa
     1501 cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctattcgccg aaaacccggt
     1561 agtgcatctc gatacggcca ccagtggcag taaatgaaac tcctgtccga caggcgctgc
     1621 gacgcgcctg ttcgcttcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc
     1681 tctcgccacc ctcccctgca ttcagcaaaa tcagactcca cggacatgta attttgataa
     1741 cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgttt ttgaatgctt
     1801 tcttatctca cgatttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga
     1861 acataccaaa acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa
     1921 ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg
     1981 caggattttg aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt
     2041 gtaattttgt tccttgaaaa cggcttgctt ccagcggcct ttttaccggg cgacagttta
     2101 ctggtattgg tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg
     2161 ctgctgaccg ttgccgccag cctcggctgc tgggtcagct atattcaggg gcgatggctg
     2221 ggcaataccc gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc
     2281 gcacaccatc tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgcg
     2341 tttgtcagaa cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt
     2401 cagtttttca actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac
     2461 atgctcggca aaacgccgat atttttaaag tacgaggacc agctgatgtc atgcctgatg
     2521 ctgctcccgg tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa
     2581 aagaaatatg gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta
     2641 gcctggtccg gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt
     2701 cgccagcaag agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac
     2761 ggtttttcga tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc
     2821 aaaaacgaca ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg
     2881 gtcctcgaca gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag
     2941 gctatgcagt ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt
     3001 atccgaaaca tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg
     3061 gtggttaaca ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca
     3121 caatggaagg ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta
     3181 gtgccggtag ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga
     3241 tcagctatgc cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga
     3301 gtgaagtccg ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg
     3361 caaagcagaa agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag
     3421 gcgatgccga taagattgcc aaacgcgaac ggaaactggc agaagcgcag gaagagctga
     3481 aaaagctgga agcgcgcgac tactaactca caatagtcac tacttactca cctggagaaa
     3541 actatgtcga aagaacacac tacggaacat ctgcgtgctg agttgaaatc cctttccgat
     3601 acgctggaag aggtgcttag ctcatctggc gagaagtcga aagaagagtt gagtaagatt
     3661 cgtagcaaag cggagcaggc actgaaacag agccgttatc gcctgggtga aaccggtgat
     3721 gccatcgcca aacaaacccg tgtcgcggcg gcgcgtgctg atgagtatgt gcgtgaaaat
     3781 ccgtggaccg gcgtgggaat tggcgctgca atcggtgtag tgctcggcgt tctgctgtcg
     3841 cgtcgttaat tatggcggac actcatcacg cgcaagggcc cggtaaaagc gttctgggca
     3901 tcgggcagcg aattgtttcc atcatggttg aaatggtgga aacacgtctg cggctggcgg
     3961 tggtggagct ggaagaggaa aaagctaatc tctttcaact tttactgatg ctgggcctga
     4021 cgatgctttt cgctgcattt ggtcttatga gcctgatggt gctaattatt tgggcggttg
     4081 atccgcaata tcgcctgaat gcgatgattg ccaccaccgt ggtgttgctg ctactggcac
     4141 tgattggcgg tatctggacg ttacgtaaat cgcgtaaatc caccttgctg cgccatacac
     4201 gccatgagct ggcaaacgat cggcaactgc tcgaggagga gtcccgtgag cagtaaagtc
     4261 gaacgtgaac gacgtaaggc gcaactgctt agccagatcc agcaacaacg gctggatctt
     4321 tccgccagtc gtcgtgaatg gctggaggca acaggcgctt acgatcgtcg ctggaatatg
     4381 ctgctaagtc tgcgctcctg ggcgctggtt ggcagtagcg tgatggcgat ctggacgatt
     4441 cgccatccta atatgctggt ccgctgggcc agacgcggtt ttggcgtatg gagcgcctgg
     4501 cgtctggtta aaacgaccct caagcagcaa cagcttcgcg gttaacttcc ctctggccgg
     4561 agcaattccg gccttatccc tcaaattttt tgaagatttt tgacagtttt ccttgctaac
     4621 aatcatcatt caccacgttt atgattctct ccatcgacag caacgacgct aataccgcgc
     4681 ccttgcacaa aaaaacaatc agcagcctga gtggcgcagt ggagagtatg atgaaaaaat
     4741 tagaagatgt tggtgtactg gtagcgcgca ttttaatgcc gattctgttt attaccgcag
     4801 gctggggaaa aatcaccgct tactctggta cccaacaata tatggaagca atgggtgtcc
     4861 cgggttttat gctgccgctg gtgattctgc ttgagttcgg tggtggtctg gcaatcctgt
     4921 tcggtttcct gactcgcacc acagccctgt ttactgcggg ctttacgctg ctgacggcat
     4981 ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     5041 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcgacc
     5101 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     5161 gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc
     5221 atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat
     5281 ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg
     5341 cagagaaaga tcgttatcat ctctatattt cactcgcctg cccgtgggcg caccgcacgc
     5401 tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc
     5461 tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc
     5521 tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac
     5581 gagttactgt tcccgtactg tgggacaaaa agaaccacac catcgtcagc aacgaatcaa
     5641 cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcgaaa gcgggtgatt
     5701 actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg
     5761 ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg
     5821 tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc
     5881 tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg
     5941 atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc
     6001 tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg
     6061 atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt
     6121 caattggtcc gtggcaggat ctcgatgaac cgcaggacga gatgttcgct tcggttaaaa
     6181 aaaggcacct tacgaagtgc ctctgataat tagctaagtg catgttgttc atatcggatg
     6241 cggcgtgaac gccttatcaa acctacaaaa catttatatt caatatgttg caatgaccgc
     6301 gcaggccaga taagcgtagc gcatctggcg attttgcgct tgtcatcagt cttagcgcgc
     6361 cttcacagca ctttattaat tccttgaacg aatatttact gccatttcat tcacgtctat
     6421 tcttaatttg ctgctttaaa aacaagtgat tgagcaacat tgaggcaaaa atggactggt
     6481 atctgaaagt aataaaaaat tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact
     6541 ggatgtttat tctggtcaac atcatcttta cgttcgtgct ggggctgctg gataaaatgt
     6601 taggctggca acgcgctggt ggcgaaggca tcctgacgac aatctacggt attctggtgt
     6661 ttttaccgtg gtgggcagtt cagttccgcc gcctgcacga caccgaccgc tcggcgtggt
     6721 gggcactgct attcttaatc ccgtttatcg gctggcttat tatcatcgtc tttaactgcc
     6781 aggcgggtac gccaggcgaa aaccgctttg gacctgatcc aaaactggaa ccttaaaaca
     6841 aatctgctcg ccggatgtct gactcatccg gcttttatca tcatgttgat gaatgaataa
     6901 tttactaatg tttatttaaa atatttcaat gtcgttatat aaattaccta taaaaaataa
     6961 ccatgtattt atcttaaata tcaaaacctt tataacatcc gaaaaagaaa gatacgaata
     7021 gcatttacac aatacccgga atagactttc gtcactttta attaaaggga tgtttttatg
     7081 cagtggtatt tatccgtatt aaaaaattat gttggtttct ctggtcgcgc acgtcgtaaa
     7141 gagtactgga tgtttactct gattaacgcc atcgtcggcg ctattatcaa tgtcattcaa
     7201 ttgattttag gtctggagct tccatatctg tctatgcttt atttgctggc aaccttcctt
     7261 cccgtccttg cgcttgctat ccgtcgctta cacgataccg accgttcagg tgcctgggcc
     7321 ttgctctttt ttgttccgtt tattggctgg ctcgtgcttc tggttttttt ctgcacagaa
     7381 ggtacttctg gcagcaatcg ttacggaaac gatccgaagt ttggttcaaa ttaatcttag
     7441 aattggggcg atatttcgcc cctttttatt aacaataata aatattattt tccgctaaaa
     7501 agtttgggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag
     7561 gccataataa tatcgatctc actggtcgat tccgggctga cgacacgcaa ccgcccttcc
     7621 gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat
     7681 gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagttgt
     7741 acggtcaaca ccgggcgctc acgagcggta tccgctaccg caattccacg atatttcacg
     7801 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg
     7861 tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc
     7921 atatccggcg cgataacaat atccgcccgc ccctgctcca gtcgttccta cgcccccgcc
     7981 agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
     8041 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
     8101 ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag caaaacgcgc
     8161 cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
     8221 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
     8281 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat cgcatccata
     8341 acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc attcaggaaa
     8401 tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc ttaccataaa
     8461 aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc agtgtggaca
     8521 agcagactac ggatggttgc aggcccggta tactttttcc tttggacact acttcgaccc
     8581 gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg ccccaggtgc
     8641 cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc tggatggaga
     8701 agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg aggcgttgct
     8761 gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag acaaaccgtt
     8821 aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc tgattcaaaa
     8881 actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta cgatgggaag
     8941 cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag gcgaaagtgc
     9001 gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga aatttcatgc
     9061 gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt ttattcgtga
     9121 cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga tagatttgcc
     9181 tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa aagcgccagc
     9241 cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt ggctatgaag
     9301 agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta gccgaggatg
     9361 aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc ggcctacaaa
     9421 atccaacaac attaacgaat taaacaaccc gtaggccgga taaggtgcgc cagcatcgca
     9481 tccggcacaa tactcaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     9541 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     9601 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     9661 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     9721 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     9781 agtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     9841 cgttgcggca caaagtgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     9901 atgagaaagc atcagcgcac gcgccagtcg ttcatcatcc gctccgaagt gttctgctac
     9961 aaccaccaca ggcattgttg cggtgatccc ctggttaccc gagccggagt tactcatagc
    10021 cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
    10081 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
    10141 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
    10201 atcgagaata aagcggatcg ccgcaaacgg gacttcattg acgaacttca ggatctcagc
    10261 cagcgtcgtt ctggaaagca ccgtcagcgg agactcttgc tcgccctctg ccacacacgc
    10321 ctgctgggta aacaccacac cattgtgcgt ctcaatacgc acaatgttgg tatgcccgcc
    10381 gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
    10441 ttcattgcaa ggttcctgga tcttaacgga gactttcccc gccgccagca gtgctttggc
    10501 atcggcaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc
    10561 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
    10621 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
    10681 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
    10741 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
    10801 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
    10861 gaacggagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
    10921 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat
    10981 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatagc
    11041 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat
    11101 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
    11161 aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
    11221 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
    11281 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgc cccatcgcca gcgtgaacga
    11341 cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
    11401 tgctttatgc cgcgccactt caatagattt ttcccgcgag cgataagaga tcaccatcgg
    11461 gcttaacgtc tggataaaca aaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
    11521 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
    11581 catcgacacg cccagcgccg cgaccaccag cagcttggtc agcaccatgc cggtcgaaat
    11641 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
    11701 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga
    11761 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
    11821 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg
    11881 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
    11941 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
    12001 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
    12061 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
    12121 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcga tttccataat
    12181 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
    12241 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
    12301 aaaatcttca caatcaagac tttgaaataa cggagatgga taagaatttt ctactaaatt
    12361 aatcgcaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
    12421 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
    12481 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
    12541 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccacgcgg
    12601 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
    12661 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
    12721 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
    12781 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
    12841 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
    12901 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
    12961 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
    13021 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
    13081 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
    13141 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aatcctgcgt
    13201 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
    13261 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
    13321 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
    13381 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
    13441 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
    13501 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
    13561 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
    13621 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccatac agatcgacca
    13681 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
    13741 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
    13801 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
    13861 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
    13921 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
    13981 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
    14041 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
    14101 cgctttgacg ttttcgaggc ttaaacacgc ctgatcttgc acatcagccg ggatctcacc
    14161 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
    14221 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
    14281 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
    14341 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
    14401 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
    14461 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
    14521 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
    14581 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
    14641 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
    14701 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
    14761 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
    14821 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
    14881 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
    14941 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
    15001 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
    15061 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
    15121 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
    15181 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
    15241 acagcagtgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
    15301 ccattgggct gacgcaacaa gcaattgcgt aatcgtcgct gttgaagtca gtacgcatca
    15361 gatcgtcgtt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
    15421 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
    15481 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
    15541 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
    15601 caaattccgg cgtacgcaag aagcgcacca tacggatctt cataatgaag tgatcgatca
    15661 gttcctgtgc ctgctgctca ttgagtacgc ctgctttaaa gtcgcgctca atgtagatat
    15721 cgaggaacga cgccgtgcgc cccagcgaca tcgcgccgcc gttctgtgat ttcactgccg
    15781 ccagataagc gaagtagagc cactgcaccg cttcctgcgc attctgcgcc gggcgagaga
    15841 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
    15901 ccagctcctc acgcagacgg atggtggctt ccagatcttc gcctttttcc agacgggact
    15961 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
    16021 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
    16081 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
    16141 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
    16201 cgtggaatga acttttaatc atattaatgc caccgaacgg atgcagcgcg cgctttaatg
    16261 gcgcatccgt ttgcagacca acaatttttt ccagcggctg gttaatatat cccgcatcat
    16321 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
    16381 cgatacggat gccttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
    16441 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
    16501 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
    16561 cgctggtatc aatatctatc ttcatgaaaa ataatctctc tacaatactt caactaaatt
    16621 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
    16681 tttcttcgtt agtcggaata acggcacaaa tgacacgcgc attttcactg gaaacaattc
    16741 gctctccaaa agagttagag cgattattca tttctgtatc aatcactacg cctaatacag
    16801 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
    16861 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
    16921 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
    16981 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
    17041 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
    17101 caaagtcgac atcgccactg cgggttccca tcatcaagcc ttccagcggc gtcattccca
    17161 ttgaagtatc aacactctga ccgttgcgaa ccgcgcagat tgacgcgcca ttgccaagat
    17221 gcgccacaac caggccggag tcatcttccg ccagattcag cagagaatgt gcgcgctggg
    17281 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    17341 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    17401 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    17461 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    17521 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    17581 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    17641 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    17701 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
    17761 cgagcacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    17821 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
    17881 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    17941 agagacggcg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
    18001 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    18061 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
    18121 ttgtcgcctt tataaccaaa cttcaggacc aggccattca gaccttccag cgttcccaga
    18181 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    18241 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
    18301 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaat
    18361 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    18421 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    18481 ttagaaacca cgaaggaaga gacgattggc gagaaattaa aggagaaaac catgatggaa
    18541 atccccagcc agacggtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    18601 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
    18661 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    18721 aaccagatga cgaaagccat cagcagcagc aggaacagcg ccacaaagcc gcgattcagc
    18781 ggtgcaaagc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    18841 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    18901 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    18961 agacatagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    19021 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    19081 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    19141 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    19201 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    19261 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
    19321 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    19381 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    19441 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    19501 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    19561 ttttcagact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    19621 ataccagcaa ttaaaccgcc accaccaatt ggcacaatca cgttatcgac atcatagaga
    19681 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    19741 ggtgggataa aaatacggcc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    19801 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    19861 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    19921 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    19981 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    20041 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    20101 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
    20161 tcaatagcaa tcggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    20221 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
    20281 taaaaacaaa taactaacca acttctatta attgccttcg tctacaccca ttataagatg
    20341 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    20401 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    20461 gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
    20521 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    20581 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    20641 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    20701 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    20761 tttctgcact taatgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    20821 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    20881 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    20941 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    21001 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    21061 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    21121 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
    21181 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    21241 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
    21301 gaccactcac aaattaactt tcaatacttt ccagggtatc gtcattacaa ttaaatacct
    21361 ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt gtcaaaaaaa
    21421 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
    21481 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
    21541 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
    21601 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
    21661 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
    21721 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
    21781 taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct gatcgccgcg
    21841 ataaggtcag acaaagacaa caagggaaat tttcacagag cttttgatcg tcgtaggcca
    21901 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgaaa
    21961 gaaccctgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
    22021 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
    22081 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tg
//
LOCUS       DAJTVK010000065        21999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000065 DAJTVK010000000
VERSION     DAJTVK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.065"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OO372_002485"
     CDS             complement(<1..988)
                     /gene="cmtA"
                     /locus_tag="OO372_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR6341953.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OO372_002486"
     CDS             complement(1016..1459)
                     /gene="cmtB"
                     /locus_tag="OO372_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR6341954.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OO372_002487"
     CDS             complement(1773..3764)
                     /gene="tkt"
                     /locus_tag="OO372_002487"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6341955.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4042..4800
                     /gene="loiP"
                     /locus_tag="OO372_002488"
                     /gene_synonym="yggG"
     CDS             4042..4800
                     /gene="loiP"
                     /locus_tag="OO372_002488"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6341956.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OO372_002489"
     CDS             complement(4943..5863)
                     /gene="speB"
                     /locus_tag="OO372_002489"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6341957.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OO372_002490"
     CDS             complement(6001..7977)
                     /gene="speA"
                     /locus_tag="OO372_002490"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6341958.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OO372_002491"
     CDS             complement(7986..8117)
                     /gene="yqgB"
                     /locus_tag="OO372_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6341959.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8253..8468
                     /locus_tag="OO372_002492"
     CDS             8253..8468
                     /locus_tag="OO372_002492"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341960.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8772..9926
                     /gene="metK"
                     /locus_tag="OO372_002493"
     CDS             8772..9926
                     /gene="metK"
                     /locus_tag="OO372_002493"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6341961.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10362..11756
                     /gene="galP"
                     /locus_tag="OO372_002494"
     CDS             10362..11756
                     /gene="galP"
                     /locus_tag="OO372_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6341962.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11833..12330
                     /locus_tag="OO372_002495"
     CDS             11833..12330
                     /locus_tag="OO372_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6341963.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12425..13132
                     /gene="endA"
                     /locus_tag="OO372_002496"
     CDS             12425..13132
                     /gene="endA"
                     /locus_tag="OO372_002496"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6341964.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13212..13943
                     /gene="rsmE"
                     /locus_tag="OO372_002497"
     CDS             13212..13943
                     /gene="rsmE"
                     /locus_tag="OO372_002497"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6341965.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13956..14906
                     /gene="gshB"
                     /locus_tag="OO372_002498"
     CDS             13956..14906
                     /gene="gshB"
                     /locus_tag="OO372_002498"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6341966.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15015..15578
                     /locus_tag="OO372_002499"
     CDS             15015..15578
                     /locus_tag="OO372_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6341967.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15578..15994
                     /gene="ruvX"
                     /locus_tag="OO372_002500"
     CDS             15578..15994
                     /gene="ruvX"
                     /locus_tag="OO372_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6341968.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16169..17149)
                     /locus_tag="OO372_002501"
     CDS             complement(16169..17149)
                     /locus_tag="OO372_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6341969.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17167..17871
                     /gene="yggS"
                     /locus_tag="OO372_002502"
     CDS             17167..17871
                     /gene="yggS"
                     /locus_tag="OO372_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6341970.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17889..18455
                     /gene="yggT"
                     /locus_tag="OO372_002503"
     CDS             17889..18455
                     /gene="yggT"
                     /locus_tag="OO372_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6341971.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18452..18742
                     /locus_tag="OO372_002504"
     CDS             18452..18742
                     /locus_tag="OO372_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6341972.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18750..19343
                     /locus_tag="OO372_002505"
     CDS             18750..19343
                     /locus_tag="OO372_002505"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6341973.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19336..20472
                     /gene="hemW"
                     /locus_tag="OO372_002506"
     CDS             19336..20472
                     /gene="hemW"
                     /locus_tag="OO372_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6341974.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            20783..21769
                     /locus_tag="OO372_002507"
                     /pseudo
     CDS             20783..21769
                     /locus_tag="OO372_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            21814..>21999
                     /locus_tag="OO372_002508"
     CDS             21814..>21999
                     /locus_tag="OO372_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6341975.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
BASE COUNT         5443 a         5716 c         5596 g         5244 t
ORIGIN      
        1 tagcaaagga caccagggta gctaccgtaa caccggcaat tgtcgccagg aacgagcctt
       61 tcggcgtcag tgccaggtaa gcaaagatag accccggact tgggccagcc accagaccac
      121 cgtccagtaa gttaaacacc caggtgccag acataccgcc cgcaatcatg gcaataatgg
      181 tcagcggctt catcagcaca tacgggaaat acagttcgtg gatcccaccg aggaagtgaa
      241 taatcatcgc cccgggcgca gaacgtttac tcatcccttt accaaacaag gtaaacgcca
      301 gcagcagccc taagccaggc ccaggattgg acgccaccat aaagaagatg gatttaccgt
      361 taaccgaagc ctgttgcatt cccagcggat aatagacgcc ctgatcgatc gcattattga
      421 ggaaaagcac tttcgccggt tcattgatga ctgacagcag aggcagataa cccgcatgta
      481 ccagcgcctc aatacactct ttgacgagag tattggcaat taacaccgcc gggccgataa
      541 cttcaaaacc cagcagacaa aggagcatcc ccgcgatacc taatgagaag ttattgataa
      601 ccatctcaaa accggcaggt atgcgttttt ccagtgcctt atcaacatat tttatgacca
      661 gcccaccgag cggccccata atcattgagc caaggaacat cgggatctct gcgccgacga
      721 tcacaccaat agtacctatt ccgcccatga ccgcgccgcg tttaccgccg accagatgac
      781 caccggtaga accaatcatc acgggcaata aataggtaat catcgggccg acaattttgg
      841 cgaaatgttc gttaggcagc caaccggtgg gaataaataa cgcagtaata aaaccccagg
      901 caataaaagc accaatattg gggatgacca ttgccgtcaa aaagccccca aaagcctgga
      961 cctttgcacg agcagacttg ttttccataa tattatcctg tagaggagag agtaattatc
     1021 cgcggctgat tatgtccgct aattgttttt ctgatgctgc tgttaagagt tgttcgagta
     1081 tttcttcttc gcacagtaat tcacttaacg cctgaatagc gccaatgtgc gaatcggcat
     1141 cggcagcaga aaggccgatg agtaatttta ttggttcgtc attccccgga aaataaacac
     1201 cttgttcaag taatgtcaaa gacatcccgg ttttaagcgc cccacattcc ggtcgcgcat
     1261 gaggcattgc cacgcctggt gcgagaatat aataagggcc attgttaatg gtggaatctt
     1321 taattgcctg aatgtaattc tcgctgatat agtttttatc cagcaatgat accatcgaga
     1381 aatcgatagc ttcctgccaa tcttttgctg aatgtataac tgagattgat gattctggaa
     1441 aatagtcact aagccgcata aattatcctt attttatttt tagggtaccg tccggcggta
     1501 aaaataaaat actcgtgcgg tttaatgaaa gcaattgagc agggaaaccg gtgttttgaa
     1561 aacagtgaat tattaatttc ttatataaca ttgagttata gatataacaa caaatgtcaa
     1621 tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat atttgtgatc
     1681 tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa taaaaaaggt
     1741 cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc tttcgcaacg
     1801 acgttgtcga cggtgaagcc gaactcttcg aacagcaact ctgccggagc tgactcaccg
     1861 aaggtggtca taccgatgat agcgccattc aggccaacat acttgtacca gtagtcagca
     1921 atacccgctt ctaccgcaac gcgtgcagta accgctttcg gcagtacgga ttcacggtaa
     1981 gccgcatcct gcttgtcgaa tgcatcggta gacggcatgg aaaccacgcg cgccttcacg
     2041 ccttcggcag tcagtttttc gtaggcggca acagccagtt caacttccga accggtcgcg
     2101 atgaagatca gttccggctg accagcacag tctttcagca cataaccacc gcgcgcgatg
     2161 tttgccagtt gctcttcagt acgttcctgc tgcgccagat tctgacggga gaggataagc
     2221 gcggtcggac cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga ttcaacctgg
     2281 tcacacggac gccatgtaga catgttcggg gtcacgcgca gagaagcgac ctgctcaacc
     2341 ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta aaccatcacc
     2401 tgacgctgtt tcatcagcgc agccatacgt acagcgttac gtgcgtattc gacaaacatc
     2461 aggaaggtgg aggtgtacgg caggaagcca ccgtgcaggg agataccgtt agcaatcgcg
     2521 gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc ttcgttgatt
     2581 gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcgga gccgccgagg
     2641 aattccggca acagcgggcc gaacgcttca atagcattct gagacgcttt acggctggcg
     2701 attttcgccg gattagcctg cagtttagcg ataaactctt tcgctttggc gtcgaagtca
     2761 gacggcattt cgcctttcat acggcgggta aattcagccg cttcctgcgg ataagctttc
     2821 gcgtaagccg caaacttctc attccatgca gattctttcg cctggcctgc ttctttcgca
     2881 tcccactgcg catagatttc agacgggatt tcgaacggtg cgtatttcca gcccagctgt
     2941 tcgcgggtca gagcgatttc tgcatcaccc agcggcgcac cgtgggagtc gtgagtaccg
     3001 gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga cggtttgtca
     3061 gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ctgcgtcatg accgtcgatg
     3121 tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc ggtgaaccag
     3181 ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat cagtttaccc
     3241 agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat catgcagccg
     3301 tcgcccatga aggcgtaggt gtagtggtcg acaatgtcgt ggcccggacg gttaaactgc
     3361 gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc ctgacccagc
     3421 ggaccggtgg tggtttccac accagcggta taacctactt ccgggtggcc cggagtttta
     3481 gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc ggtgaggtgc
     3541 agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg gtcacggtca
     3601 gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac ttcggcaatg
     3661 tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac tgcgtccatg
     3721 ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac tccagatcgg
     3781 atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta cgccggaaaa
     3841 tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt ttgccgcttc
     3901 agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg ctgcgctttt
     3961 ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag aataatcctg
     4021 accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat gagcgtggca
     4081 acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc aggagcggaa
     4141 gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga tcaggcatgt
     4201 caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc taaacgtctg
     4261 acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa ttacaaagtg
     4321 tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg cgtctatagc
     4381 gggctgatgg atatgatgac agataacgaa gtcgaagcgg tgatcggtca cgagatgggg
     4441 cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac aaatgccgtg
     4501 cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc acaacttggt
     4561 gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc agaagccgat
     4621 gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct tgccaccagc
     4681 tttgaaaaac tggcgaaact ggaagaaggc cgccaaagct caatgtttga cgaccatccg
     4741 gcatccgccg aacgcgccca gcatattcgc gatcgcatga gcgcggatgg gattaagtaa
     4801 agcctggtgg tgtcgaacgt aggtcagata aggcgttcac gccgcatctg acatcaatgg
     4861 cacatttgtt accctgtgcg cattgccgga tgcgatgctg actgcatctt atccggccta
     4921 cgggtggcat cgcatctggt gcttactcgc cctttttcgc cgcttggata tacagcatct
     4981 ccagcgccag cgtcgccgca gccagagcgg tgatttccga ctgatcgtat gccggagcca
     5041 cttccactac gtccatccca acaatgttga gatctttcag gccgcgtacc agtttaatag
     5101 cgcgatcgga ggtcaggccg ccaatcactg gcgtaccggt gcctggtgca aaagcaggat
     5161 ccaggcagtc gatatcaaaa gtcaggtaaa ccggcatatc acccacaatc tgtttcactt
     5221 gggcgataac gtcatccacg ctgcgatcgt tcacctggca ggcgtccagc acggtaaagc
     5281 cgttgtcttt atcaaactcg gtacgaatac caatctgcac ggaatgattc gggtcgatca
     5341 gaccttcttt cggcgcggta tagaacatag tgccgtggtc aaattcacaa ccgttcgcat
     5401 aggtatcggt gtgggcgtca aagtgtacca gcgccatttt gccgaaatgc ttcgcatgag
     5461 cacgcagcag cggcagcgta acaaagtggt caccaccgaa ggtgagcata cgcttaccgg
     5521 cagccagcag cttctcggcg tgcgcctgca gcttttcgct catctcacgg gcatcgccaa
     5581 aggcatatac cagatcgccg cagtccacga cgttcagacg ctcacgcatg tcgaaattcc
     5641 acgggaagcg gttgtgttcc caggccagat tcgtcgaaac ctgacggatc gctgccggac
     5701 cgtggcgacc acccgcacga ccagaagtgg ccatatcgaa cggcacgcca gtaatcaccc
     5761 agtctgcatc gctgtcatac ggctggaagt tcatcggcag gcgtaaaaaa ccaaaggcat
     5821 tggaaaccag tgagttatcg tattgatgac ctaaggtgct catggatgga ctcctattac
     5881 aaagtcgata taaaaaaacc cttccgcgtc gttaggcccg acgaggaagg gttggatttg
     5941 tcacaataaa ttgtggcgga ttatcaccgc taaattaagc ggattcaagt aacacaggac
     6001 ttactcatct tcaagataag tataaccgta caaacctgcc tcgaactctt caaggaactg
     6061 ttgttgcagt tcagcatcaa gatcggtttt cttcacttga tcgcggaact gggttaacag
     6121 cgttttcgga tcgagctgta catattgcag catgtccgcc acggtatcgc cttcgtcaga
     6181 cagttctact tctacgctac cgtcagggaa cacgaacacg tcaaccgctt cggtatcacc
     6241 gaacaggttg tgcatattgc caaggatctc ctgatatgcg ccgaccataa agaaaccgag
     6301 catcggcgga ttctctggat cgtactccgg cattggcatt gtcgtggcaa taccatcacc
     6361 atcaatatag tggtcgatag caccgtcaga gtcacaggta atatccagca gcacagcgcg
     6421 acgttccggc acttgatcca gcccttccag cggcagaacc gggaacaact ggtcgatccc
     6481 ccatgcgtcc ggcattgact ggaacagcga gaaattgacg tacattttgt ccgccatacg
     6541 ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt tgcggatcca gttgcttctg
     6601 cacttcatgg cacatgctca gatagagttg ttctgcccat gcacgttctt gcaggctaaa
     6661 ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc atctggctgt cgtgtaacca
     6721 ttcacgcaaa gaacggcgag ttcccggttc gtgcatttcc tgccaggttt cccacatgct
     6781 ttgcagcgcg cgcggcgcat cttctgcggg cgcggtcggc accgtatatt cgttacgttc
     6841 cacgccgatg atattcgaca ccagcacggt atggtgcgca gtcaccgcac ggcccgattc
     6901 ggtgattacc gtcggatgcg gcagaccatt ttcttcacac gcatcgccaa tcgcccagat
     6961 gatgttgttg gcgtattcat tgaggccata gtttacggag cagtcagact gcgaacgggt
     7021 gccttcatag tccacgccca gaccgccgcc gacgtcgaag cactgaatat tgacgcccag
     7081 cttgtgcagt tccacataga aacgcgcgga ttcacgaacg cccgtggcga tatcgcgaat
     7141 attcgccatc tgcgaaccga ggtggaagtg cagtagttgc aggctgtcga gacgcccggc
     7201 ttcacgcagg gtttcaacca gttgcagtac ctgagtcgca gccaggccga acttcgattt
     7261 ttccccgccg gaggactgcc atttacccga gccctgcgaa gccagacgtg cacgcacgcc
     7321 cagacgagga acgacattca gacgttctgc ttcatccagc acaatggcga tttctgacat
     7381 cttctcaatg accagataga ccttgtgccc catcttctcg ccaattaatg ccaggcggat
     7441 atattcgcgg tctttataac cgttgcagac gatgacgcta cgggtcatgc cagcatgtgc
     7501 cagtactgcc atcaactcgg ctttggaacc ggcttccaga cccagcggtt cgcccgaatg
     7561 aatcagggac tcaatcacgc ggcggtgctg gttaactttg atcggataaa caaggaagta
     7621 atcgccgtta tagccgtagg attccctcgc acgtttgaac gcggcgttaa tggaacgcaa
     7681 acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc agacgctgac cctgtgcttc
     7741 acgagttttc actaactgcg cgagatcgac gcgagcttcc gggacgtccg ggtccgggca
     7801 cacgctgatg tggcccagct cgttaacgtc atagtagtta ttgccccacc aggcaatatt
     7861 gtaagtacgc agcatcttgc tggcttcctg ggagctcatt gcaacctcct gcatggagcg
     7921 tagtacaccg tgttcgcccg ctgacgaagg caaacccata gacatgtcgt cagacatagc
     7981 gaacctcaaa ttattttatt aagtgtaaaa cagttaacga ctatcgcagc ctggaactgc
     8041 gataacaacc cataagcaca tggattttcc agcagtgaat gctgacgctc caactgcgca
     8101 accggtttct ttttcataac attattaagc acataaccga acgtaagtgt gacagttcgg
     8161 cgaaaccacg agaaaactct tgtttttaca agagcgccct tgttcagtcc tcagtaactg
     8221 taaccagctc ttgaatcctg agaagcgccg agatgggtat aacatcggca ggtatgcaaa
     8281 gcagagatgc agagtgcggg gaacgaatct tcaccagaac ggtgagacag gttaagcagc
     8341 agacaacggt tcattatttc gtatcacctc cacggccgcc tgttaagacg aacccacaag
     8401 ccaaaactct gatttcaacc cggctggaag tggcaacacg aaagaaacgt cgtgtgcttt
     8461 ttatttaagc cgcgcgccgc gttttatacc ccacaatggc agaaaattgc aaaagataaa
     8521 tacgcagaat gccggcattg tcaggaaaaa tttccagcca cgtttttaac agaatgagac
     8581 acgattcaaa aaaaagtgga aatcgggtga agaattgacc taaaatagcc atccagatgt
     8641 taatccatcc ataccgatta acactcagac tgccagtgtt tttaacctgc agagtcgtgg
     8701 taggatccgc taccacagaa aatccacaca acagtttgag ctaaccaaat tctctttagg
     8761 tgatattaaa tatggcaaaa caccttttta cgtccgagtc cgtctctgaa gggcatcctg
     8821 acaaaattgc tgaccaaatc tctgatgccg ttttagacgc gatcctcgaa caggatccga
     8881 aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat ggttttagtt ggcggcgaaa
     8941 tcaccaccag cgcctgggta gacatcgaag agatcacccg taacaccgtt cgcgaaattg
     9001 gctatgtgca ttccgacatg ggctttgacg ctaactcctg tgcggttctg agcgctatcg
     9061 gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc cgatccgctg gaacagggcg
     9121 cgggtgacca gggtctgatg tttggctacg caactaatga aaccgacgtg ctgatgccag
     9181 cacctatcac ctatgcacac cgtctggtac agcgtcaggc tgaagtgcgt aaaaacggca
     9241 ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac tttccagtat gacgacggca
     9301 aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca ctctgaagag atcgaccaga
     9361 aatcgctgca agaagcggta atggaagaga tcatcaagcc gattctgccc gctgaatggc
     9421 tgacttctgc caccaaattc ttcatcaacc cgaccggtcg ttttgttatc ggtggcccga
     9481 tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga tacctacggc ggcatggcac
     9541 gtcacggtgg cggagcattc tctggtaaag atccatcaaa agtggaccgt tccgcagcct
     9601 acgcagcacg ttatgtcgcg aaaaacatcg ttgctgctgg cctggccgat cgttgtgaaa
     9661 ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc cattatggta gaaactttcg
     9721 gtactgagaa agtgccttct gaacaactga ctctgctggt acgtgagttc ttcgacctgc
     9781 gcccatacgg tctgattcag atgctggatc tgctgcaccc gatctacaaa gaaaccgcag
     9841 catacggtca ctttggtcgt gaacatttcc cgtgggaaaa aaccgacaaa gcgcagctgc
     9901 tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg ctttcaaagg ccagcctcgc
     9961 gctggccttt ttcttttacc tcaccgcggt ggataggcat tcacgccgca tccggcaaaa
    10021 agctgcccgc acaataacat cattcttcct caacacgttt tatcgcaaat tatcatcact
    10081 actgaaaccg attacaccaa taacaccagc caaatatttg taatattttc atattattat
    10141 tcagttttca cagttgttac atttcttttc agtaaagtct taattgcaga taacagcgtt
    10201 taatctatga tgatataact caattatttt catgcactta aaccataact aagataaatg
    10261 ttagtgtaag cgattacact gatgtgattt gcttcacatc tttttacgtc gtactcacct
    10321 atcttaattc acaataaaaa ataaccatat tggagggcat catgcctgac gctaaaaaac
    10381 aggggcggtc aaacaaggca atgacgtttt tcgtctgctt ccttgccgct ctggcgggat
    10441 tactctttgg cctggatatc ggtgtaattg ctggcgcact gccgtttatt gcagatgaat
    10501 tccagattac ttcgcacacg caagaatggg tcgtaagctc catgatgttc ggtgcggcag
    10561 tcggtgcggt gggcagcggc tggctctcct ttaaactcgg gcgcaaaaag agcctgatga
    10621 tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc ggctgcgcca aacgttgaag
    10681 tactgattct ttcccgcgtt ctactggggc tggcggtggg tgtggcctct tataccgcac
    10741 cgctgtacct ctctgaaatt gcgccggaaa aaattcgtgg cagtatgatc tcgatgtatc
    10801 agttgatgat cactatcggg atcctcggtg cttatctttc tgataccgcc ttcagctaca
    10861 ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc ggcaattttg ctgctgattg
    10921 gtgtcttctt cctgccagac agcccacgtt ggtttgccgc caaacgccgt tttgttgatg
    10981 ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga agcgaaacgc gaactggatg
    11041 aaatccgtga aagtttgcag gttaaacaga gtggctgggc gctgtttaaa gagaacagca
    11101 acttccgccg cgcggtgttc cttggcgtac tgttgcaggt aatgcagcaa ttcaccggga
    11161 tgaacgtcat catgtattac gcgccgaaaa tcttcgaact ggcgggttat accaacacca
    11221 cagagcaaat gtgggggaca gtgattgtcg gcctgaccaa cgtacttgcc acctttatcg
    11281 caatcggcct tgttgaccgc tggggacgaa aaccaacgct aacgctgggc ttcctggtga
    11341 tggctgctgg catgggcgta ctcggtacaa tgatgcatat cggtatccac tctccgtcgg
    11401 cgcagtattt cgccatcgcc atgctgctga tgtttattgt cggttttgcc atgagtgccg
    11461 gtccgctgat ttgggtactg tgctccgaaa ttcagccgct gaaaggccgc gattttggca
    11521 tcacctgctc cactgccacc aactggattg ccaacatgat cgttggcgca acgttcctga
    11581 ccatgctcaa cacgctgggt aacgccaaca ccttctgggt gtatgcggct ctgaacgtac
    11641 tgtttatcct gctgacattg tggctggtac cggaaaccaa acacgtttcg ctggaacata
    11701 ttgaacgtaa cctgatgaaa ggtcgtaaac tgcgcgaaat cggcgctcac gattaatctc
    11761 ctcaggcttc ctcccgtcgc ggaggaagcc gcctcttgca gtcatctttt cttcgctcta
    11821 tcctctgccg ctatgaaaac atcccgtctc cctatcgcca tccaacaggc cgttatgcgc
    11881 ggcctgcggg aaaaactcgc ccaggccaac ctgaagctag ggcgtaacta cccggagcca
    11941 aaactctctt acacccagcg cggaacctcc gccggaacag cctggctgga aagctatgaa
    12001 atccgcctca atcccgtttt gctgttggaa aacagtgaag cttttattga agaagtggta
    12061 ccgcacgaac tggcacattt gctggtatgg aaacatttcg gccgcgtagc gccacatggc
    12121 aaagagtgga agtggatgat ggaaagcgtg ctgggtgttc ccgcccgtcg tacgcatcag
    12181 ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc gctgcaagtg ccaggagcat
    12241 cagcttaccg tacgccgcca taatcgcgta gttcgtggcg aggccgtcta tcgctgtgtt
    12301 cactgcggtg aacagctggt tgcgaaataa ccatctgaac tatcaggaac tttcctgatc
    12361 tggctgattg cataccaaaa caactttcgc tacgttgctg gctcgtttta acacggagta
    12421 agtgatgtac cgttatttgt ctattgctgc ggtggtactg agcgcagcat tttccggccc
    12481 ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa gccgcggcgg taaaagtcca
    12541 cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt aactggcagg gcaaaaaagg
    12601 cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa aatgaaaacc gagccagccg
    12661 cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt caccagcgcc agtgctggca
    12721 ggacggtgga cgtaaaaact gcgctaaaga tccggtctat cgcaagatgg aaagcgatat
    12781 gtataacctg cagccgtcag tcggtgaggt gaatggcgat cgcggcaact ttatgtacag
    12841 ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc atgaaggtcg atttcaaaga
    12901 aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt gcgcgcacct acttctatat
    12961 gcgcgaccaa tacaacctga cactctctcg ccagcaaacg cagctgttca acgcatggaa
    13021 caagatgtat ccggttaccg actgggagtg cgagcgcgat gaacgcatcg cgaaggtgca
    13081 aggcaatcat aacccgtatg tgcaacgcgc ttgccaggcg cgaaagagct aacctacact
    13141 agcgggattc tttttgttaa cccctacccc acgcgtacaa ccgcgtgggg agacgacgcg
    13201 gatttttaac tatgcgtatc ccccgcattt atcatcctga accactgacc agccattctc
    13261 acatcgcgct ttgcgaagat gccgccaacc atatcgggcg cgtactgcgc atggggccgg
    13321 ggcaggcgtt gcaattgttt gacggtagca accaggtctt tgacgccgaa attaccagcg
    13381 ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa gatcgacgat cgcgaatctc
    13441 cgctgcatat tcacctcggt caggtgatgt cgcgtggtga aaaaatggaa tttacgatcc
    13501 agaaatcgat cgaactcggt gtaagcctca ttacgccact tttttctgag cgctgcggcg
    13561 ttaaactgga tagtgaacgt ctgaacaaga agctccagca gtggcagaag attgcaattg
    13621 ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat ccgtccagcg atggatctgg
    13681 aagcctggtg tgcagagcag gatgaaggac tgaaactgaa tcttcacccg cgcgccagta
    13741 acagcatcaa tacgttgccg ttaccggttg aacgcgtccg cctgctgatt ggcccggaag
    13801 gcggtttatc ggcagatgaa attgccatga ctgcccgcta tcaatttact gatatcctgt
    13861 tgggacctcg cgttttgcgt acagagacaa ctgcgctcac cgccattacc gcgctacaag
    13921 tacgatttgg cgatttgggc taacggagaa gaataatgat caagctcggc atcgtgatgg
    13981 accccatcgc aaacatcaac atcaagaaag attccagttt cgctatgttg ctggaagcac
    14041 agcgtcgtgg ttacgaactt cactatatgg agatgggcga tctgtatctg atcaatggcg
    14101 aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa ctacgaagag tggttttcgt
    14161 tcgtcggtga acaggatctg ccgctggccg atctcgatgt gatcctgatg cgtaaagacc
    14221 cgccgtttga taccgagttt atctacgcga cctatattct ggaacgtgcc gaagagaaag
    14281 ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg taacgagaaa ctgtttaccg
    14341 cctggttctc tgacttaacg ccagaaacgc tggttacgcg caataaagcg cagctaaaag
    14401 cgttctggga gaaacacagc gacatcattc ttaagccgct ggacggtatg ggcggcgcgt
    14461 cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt gattgccgaa accctgactg
    14521 agaatggcac tcgctactgc atggcacaaa attacctgcc agccattaaa gatggcgaca
    14581 aacgcgtgct ggtggtggat ggcgagccgg taccgtactg cctggcgcgt attccgcagg
    14641 ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg tgaacctcgt ccgctgacgg
    14701 aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgct gaaagaaaaa gggctgattt
    14761 ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat taacgtcacc agcccaacct
    14821 gtattcgtga gattgaagca gagtttccgg tgtcgatcac cggaatgtta atggatgcca
    14881 tcgaagcacg tttacagcag cagtaaccca ccttagcgag aaggatctcg ttgagactct
    14941 gagtgacagc gcccttcttt ccacgcatac tgggcgctgt tgcttttttg aaccaggaaa
    15001 cagaacctct gacaatgaat ttacagcatc actttcttat tgccatgcct gctctccagg
    15061 atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca taataccaat ggtgcaatgg
    15121 ggatcatcgt caacaagccg ctggaaaatc tcaaaattga agggattctg gaaaagctga
    15181 agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga taaaccggtt atgctcggcg
    15241 gtccgctggc tgaagatcgc gggtttattt tgcatactcc gccctccaat tttgcttcca
    15301 gcattcgcat ttcagacaac acagtgatga ccacttcccg cgatgtgctg gaaacgctcg
    15361 gcaccgataa acaaccgtct gacgtattgg tggctctagg ttatgcctcc tgggaaaaag
    15421 gtcaactgga acaagaaatt ctcgataacg cgtggctaac ggccccggcg gatctgaata
    15481 ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc ggcaaaactg attggtgtgg
    15541 atattctcac catgcctggt gtggcagggc acgcctgatg agtggaacct tactcgcctt
    15601 cgacttcggc accaaaagca ttggcgtagc ggtcggccaa cgcattaccg gcactgctcg
    15661 ccctttgcct gcaattaaag cacaggacgg tacgccggac tggaacctca tcgaacgttt
    15721 actgaaagag tggcagccgg acgaaatcat cgtcggtttg ccgctgaata tggacggcac
    15781 cgagcaacca ttgactgcca gagcgcgtaa atttgccaac cgtattcatg gtcgtttcgg
    15841 tgttgaagta aagctccatg acgaacgtct tagcaccgtg gaagcccgtt ccggtctgtt
    15901 tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc gactctgcct ctgcggttat
    15961 tattctcgaa agctatttcg agcagggata ttaaggcgat ttaaaagcct ggcggatagt
    16021 cgttaccgcc atcttgccgg atgcggcgtg aacgcctatc cggcctacat gtggcagcga
    16081 ttgtaggtct gataagacgc gcaaacgtct catcagacgt ttattgcctt tgtaggcccg
    16141 ataagcttgc acatcgggca tggcaacgtc acaaacgccc ttcccccact cgctgctgaa
    16201 aactctgctg aaacgttatc atccccacct gctgcccggt ttgaataaca tgcggtaact
    16261 ggtgggtttt cccttcgcga atcaaattcc ccaccgcggg agtgttaatc aataattcaa
    16321 acagcgccac gcgtccttcc tgtttatcca cttccagctt ttgtgacaac acggcccgta
    16381 aactacctgc cagttgatta cgtacgggat ctttttcctg cgccggaaat gaatccacca
    16441 gtcgctcaac tgcctgcgcg gcaccacgcg tatgtaatgt tgccagcacc aaatgcccgg
    16501 tttctgccgc cgtcagtgcc agacggattg tctcgctgtc acgcagctct ccgagcaaaa
    16561 tcacatcagg atcttcccgc aatgcggccc gcaatcccga tgcgaacgtc atacagtgca
    16621 aaccaatttc ccgcttctgg atcaaacatc gctggctggc atagatatat tcaacaggat
    16681 cttccagcgt cagaatatgc gcatcggcat gttgattgag atagccaacc atcgccgcca
    16741 gcgtggtaga tttgctgctc cccgtcgccc ccgtcaccag aatcaggcca ttctcactct
    16801 tgagtaattc cggcaatacc gttggtgcgc caagctgttc gagctgcggg cagtgcgaag
    16861 gtaacagccg taacgccagc gaaatgccat gccgttgcgc aaatgcgctg ccacgcaatc
    16921 gctggttttc cgccagcgac acggcaaaat ccagctggcc attttccagc aatattgccc
    16981 gctgatcgtc atccagccac tcccgcagta gctcttcgac gtccggcgcc tcaaacggcg
    17041 cagcttccat tagcccgcga atgcgccatc gtgcgggcca ggcgctgcac aggtgtagat
    17101 ccgagacgtt atgctttaca ctaagggcca caatttcttc catattcata ctaagatcct
    17161 cggaaaatga acgatattgc gcataacctg gcacaggtcc gggacaaaat ctcagcggct
    17221 gcaacgcgtt gcggccgttc tccagaagaa attacgctgc ttgcagtcag taaaacaaaa
    17281 cctgcgagcg ccatcgcaga agccattgat gccgggcagc gtcaatttgg tgaaaactac
    17341 gttcaggaag gggtagataa aattcgccac tttcaggaac tgggcgtaac aggattagaa
    17401 tggcatttta ttggcccgtt gcagtctaat aaaagccgcc tggtggcaga gcatttcgac
    17461 tggtgtcata ccatcgaccg tttgcgcatt gctacccgcc tcaacgatca gcgcccggca
    17521 gaacttcccc ctcttaacgt actgattcaa attaatatta gtgatgaaaa cagtaagtcc
    17581 gggattcaac tggcagaact ggacgagctg gcagcggcgg tcgctgaact accgcgttta
    17641 cgtctgcgcg ggctgatggc aatccctgcg cctgagtcag aatatgtaag gcagtttgaa
    17701 gttgcacgcc aaatggctgt agcatttgcc ggactgaaaa cgcgctaccc gcatatcgac
    17761 acgctctctc tgggaatgtc ggacgatatg gaagccgcca ttgcggcagg tagcacgatg
    17821 gttcgtatcg gcactgcaat ttttggtgcg cgtgattact ctaaaaaata aggaattaaa
    17881 ggaacgccat gaatacgttg actttcctgc tttcaacggt cattgagctg tataccatgg
    17941 tgctgttatt acgcatctgg atgcagtggg ctcattgtga tttttacaac cccttctcac
    18001 agtttgtagt gaaggtaacg cagccaatta tcgggccact gcgccgcgtt attccggcaa
    18061 tgggccccat tgacagcgcc tcgctgctgg ttgcctatat tctcagtttt atcaaagcca
    18121 tcgtgctgtt taaagtggtg accttcctgc caatcatctg gattgctggt ttactgattc
    18181 tgctgaaaac catcggcctg ctgattttct gggtcctgct ggtgatggcg attatgagct
    18241 gggtaagcca gggtcgtagt ccgattgaat acgtgctgat tcagctggcc gatccgctgc
    18301 tgcgcccgat tcgccgcctg ctaccggcaa tgggtgggat tgatttctcg ccgatgatcc
    18361 tcgttctgct gctgtatgtc atcaatatgg gtgtcgcaga agtattacag gcaaccggaa
    18421 acatgctgct gccggggctg tggatggcgt tatgagtgcc gtaacagtta atgatgacgg
    18481 tctggtttta cggctctata ttcagccgaa agccagccgt gattctattg tcggtttaca
    18541 tggcgacgaa gttaaagtcg ccattaccgc gccgccggtt gacggccagg gcaacagtca
    18601 tctggtgaag tttctcggta agcaattccg ggttgccaaa agccaggtgg tgattgaaaa
    18661 aggcgaactt ggccgccaca aacaaattaa aatcattaat ccgcaacaaa tcccgccaga
    18721 aatcgcggcg ttaattaatt aggtatccta tgcaaaaagt tgtcctcgca accggcaatg
    18781 ccggtaaagt gcgtgagctg gcgtcgctgc ttagcgactt cggtcttgat atcgtggccc
    18841 aaacagacct cggcgtagat tccgctgaag aaaccggcct gacctttatc gaaaacgcga
    18901 ttctgaaagc gcgctatgcg gcaaaagtga ccggtttacc ggcaattgcc gacgactctg
    18961 gtctggcggt agatgcgctg ggtggcgcgc cgggtattta ctccgcgcgt tattccggtg
    19021 aagacgcgac cgatcaaaag aatctgcaaa aactgttgga aacactgaaa gacgtaccgg
    19081 acgaccaacg tcaggcgcgt ttccactgcg tgctggtata tctgcgtcac gcggaagatc
    19141 cgactccgct ggtatgccac ggcagctggc cgggcgtgat tactcgcgaa ccagcgggca
    19201 ctggtggctt tggttatgat ccaatcttct tcgtaccttc cgaagggaaa accgccgccg
    19261 aactgacccg cgaagaaaag agcgccattt cccaccgtgg tcaggcgttg aaactgctgc
    19321 tggacgcttt acgtaatggt taaattaccg ccgctgagtc tctacattca catcccgtgg
    19381 tgcgtgcaga aatgcccgta ctgcgatttc aactctcacg cgttgaaagg agaagtgccg
    19441 cacgacgatt atgttcagca tctgctttgc gatctggaca atgatgtggc ttacgctcag
    19501 ggccgtgaag taaagacaat ctttattggc ggtggtacgc cgagcctgct ttccggcccg
    19561 gcgatgcaaa cgctgctgga cggcgtgcgt gcgcgtttgc cgctggcagc ggatgcagaa
    19621 attactatgg aagcgaaccc tggaacggta gaagccgatc gctttgtcga ttatcagcgt
    19681 gctggtgtga accgcatctc tattggtgtg cagagtttta gcgaagaaaa gctgaaacga
    19741 cttgggcgta ttcatggccc gcaagaagcg aaacgcgcgg caaagctggc gagcgggctg
    19801 gggctgcgta gctttaacct cgatttgatg catggactac cggatcaatc actggaagag
    19861 gcgcttggcg atctacgcca ggccattgaa ctgaatccgc cgcatctttc ctggtatcaa
    19921 ctgaccatcg aacccaatac gctgtttggt tcgcgaccac cggtgctgcc ggacgatgac
    19981 gcgctgtggg atattttcga acaggggcat cagttattaa ccgcagcggg ttatcagcaa
    20041 tatgaaactt ccgcttacgc caaacccggt tatcagtgcc agcacaatct caactactgg
    20101 cgatttggtg actacatcgg tattggctgc ggcgcgcacg gcaaagtcac cttcccggat
    20161 gggcgcattc tgcgtaccac taaaacgcgt catcctcgtg gttttatgca aggaaggtat
    20221 ctggaaagcc agcgtgatgt cgaagccgca gataagccgt ttgagttctt tatgaatcgc
    20281 ttccgtctgc tggaggccgc gccgcgcgtg gagtttattg cgtataccgg gctttgcgaa
    20341 gatgtgattc gcccacagtt agacgaggcg attgcccagg gttatctcac cgaatgtgcg
    20401 gattactggc agataaccga gcacggtaaa ttattcttaa actcacttct tgaattattc
    20461 ctcgctgagt aatcattaat gcgcctgcgg gcgcattaat cacttctttt ccccccgtta
    20521 attaacttcg gcaaaatata tttcttactg aataaacatc gatgatattt attagatata
    20581 tctagtgaat atttactcat catttattga ttataaacat gtacaacctg gcgcacaaat
    20641 ttaatattta atttctgact ttatttcaag ttgtgataaa catagacaag ttgtcagata
    20701 ggttaaccaa tttgtcgcac aaagcactga taagcacaat tagatatcaa ccaatttcca
    20761 tccttcaaca gggagttttt ttatgaaaat gaggtcattt acccgctcat tagtctgcgc
    20821 atccttgctg gctctggtta gcactggcgt aaacgccgca gaaaaagtga cgttaaagtt
    20881 agcacacaac ctggaaagaa gtcatgttgt acatcagagt tttgaagagc tggcgaaaga
    20941 ggtaaaacag ctgtcagaag gtaatatggt gatccgtatt tatcccagca gccagatggg
    21001 taatgcgcga taaactatgg aattgttgca aaatggcgca ctggatatga ccaaaggttc
    21061 cgccagtgat ctggagtctt ttgataatat ttatgctatc tataatttgc cgttcttatt
    21121 taaagatcag gctcacttca acaaagttgt ttttggtgaa gttggtaaag aaattatgga
    21181 ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta cacgcagttt
    21241 ctatgcgaaa aaaccgatta ctaagccaga agatcttaaa gggctgaaaa ttcgtgttca
    21301 gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca ctccaatttc
    21361 atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag aaaataacgt
    21421 gccttcctgg gtgcagactc gacatattga aattgccaaa gttttttctg aagatgaaca
    21481 cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat taaccccaga
    21541 acagaaacaa atcctcgaaa cagcagcgaa gaaatccgaa gcttatcaac aaaaattgtg
    21601 ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg aaattgtaaa
    21661 agtggataaa gcaccattcc gtgccgcagt tcagcctctg tttgacgact tcaaaaaaga
    21721 tccaaaacaa gctgctttgt tagagaagtt cgacaacgct gctcaataat attccgggcc
    21781 agtctctggc cctgattttc caagatgctt acaatgatat ttaaccgcct gaaacttgcg
    21841 gtagaccgcg tcattgccgc gttttctgtc gccgtcatgc ttgctctggt tgtatgcgta
    21901 gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac cgatgagctg
    21961 gcacgatttt tgatgatctg ggtagggtta ttaggtgct
//
LOCUS       DAJTVK010000066        21643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000066 DAJTVK010000000
VERSION     DAJTVK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.066"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OO372_002509"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="OO372_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6341976.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="OO372_002510"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="OO372_002510"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6341977.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OO372_002511"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="OO372_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6341978.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="OO372_002512"
     CDS             complement(3717..4511)
                     /locus_tag="OO372_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6341979.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="OO372_002513"
     CDS             4649..4990
                     /locus_tag="OO372_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6341980.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OO372_002514"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="OO372_002514"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6341981.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="OO372_002515"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="OO372_002515"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6341982.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="OO372_002516"
     CDS             8906..10198
                     /locus_tag="OO372_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6341983.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="OO372_002517"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="OO372_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6341984.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="OO372_002518"
     CDS             complement(10731..11189)
                     /locus_tag="OO372_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6341985.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="OO372_002519"
     CDS             complement(11186..12103)
                     /locus_tag="OO372_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6341986.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="OO372_002520"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="OO372_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6341987.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="OO372_002521"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="OO372_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6341988.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OO372_002522"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="OO372_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6341989.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OO372_002523"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="OO372_002523"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6341990.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OO372_002524"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="OO372_002524"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR6341991.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="OO372_002525"
     CDS             16063..16749
                     /locus_tag="OO372_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6341992.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="OO372_002526"
     CDS             16746..19160
                     /locus_tag="OO372_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6341993.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OO372_002527"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="OO372_002527"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6341994.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OO372_002528"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="OO372_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAJTVK010000067        21553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000067 DAJTVK010000000
VERSION     DAJTVK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.067"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1365
                     /gene="espL1"
                     /locus_tag="OO372_002529"
                     /pseudo
     CDS             1..1365
                     /gene="espL1"
                     /locus_tag="OO372_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1537..1644
                     /locus_tag="OO372_002530"
     CDS             1537..1644
                     /locus_tag="OO372_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6341995.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1697..2455)
                     /locus_tag="OO372_002531"
     CDS             complement(1697..2455)
                     /locus_tag="OO372_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6341996.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2742..3671
                     /gene="pfkB"
                     /locus_tag="OO372_002532"
     CDS             2742..3671
                     /gene="pfkB"
                     /locus_tag="OO372_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6341997.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3772..4062
                     /gene="ghoS"
                     /locus_tag="OO372_002533"
     CDS             3772..4062
                     /gene="ghoS"
                     /locus_tag="OO372_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6341998.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            4168..5028
                     /locus_tag="OO372_002534"
     CDS             4168..5028
                     /locus_tag="OO372_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6341999.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5069..5605)
                     /locus_tag="OO372_002535"
     CDS             complement(5069..5605)
                     /locus_tag="OO372_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6342000.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5752..6420
                     /gene="hxpB"
                     /locus_tag="OO372_002536"
     CDS             5752..6420
                     /gene="hxpB"
                     /locus_tag="OO372_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6342001.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6583..7173
                     /locus_tag="OO372_002537"
     CDS             6583..7173
                     /locus_tag="OO372_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6342002.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            7306..8697
                     /gene="tcyP"
                     /locus_tag="OO372_002538"
     CDS             7306..8697
                     /gene="tcyP"
                     /locus_tag="OO372_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR6342003.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(8701..9517)
                     /locus_tag="OO372_002539"
                     /pseudo
     CDS             complement(8701..9517)
                     /locus_tag="OO372_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OO372_002540"
     CDS             complement(9794..10057)
                     /gene="cedA"
                     /locus_tag="OO372_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6342004.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            10240..12501
                     /gene="katE"
                     /locus_tag="OO372_002541"
     CDS             10240..12501
                     /gene="katE"
                     /locus_tag="OO372_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6342005.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OO372_002542"
     CDS             complement(12759..13517)
                     /gene="chbG"
                     /locus_tag="OO372_002542"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6342006.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OO372_002543"
                     /gene_synonym="chbF"
     CDS             complement(13530..14882)
                     /gene="celF"
                     /locus_tag="OO372_002543"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6342007.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OO372_002544"
     CDS             complement(14987..15829)
                     /gene="chbR"
                     /locus_tag="OO372_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR6342008.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OO372_002545"
     CDS             complement(15837..16187)
                     /gene="chbA"
                     /locus_tag="OO372_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6342009.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OO372_002546"
     CDS             complement(16238..17596)
                     /gene="chbC"
                     /locus_tag="OO372_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR6342010.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OO372_002547"
     CDS             complement(17681..18001)
                     /gene="chbB"
                     /locus_tag="OO372_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6342011.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OO372_002548"
     CDS             complement(18300..18638)
                     /gene="osmE"
                     /locus_tag="OO372_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6342012.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            18840..19667
                     /gene="nadE"
                     /locus_tag="OO372_002549"
     CDS             18840..19667
                     /gene="nadE"
                     /locus_tag="OO372_002549"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6342013.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            19897..20784
                     /gene="cho"
                     /locus_tag="OO372_002550"
     CDS             19897..20784
                     /gene="cho"
                     /locus_tag="OO372_002550"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6342014.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OO372_002551"
     CDS             complement(20744..21319)
                     /gene="ves"
                     /locus_tag="OO372_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6342015.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         5738 a         5314 c         4938 g         5563 t
ORIGIN      
        1 cccttgattc attgagaaat ggaaaaggca aagtcaacct gattaaacac tactcctccg
       61 ttgaatccat acagcagcat gtccccttag tccgggacgc ggagttcaga gcattacttc
      121 gccatcctcc tgcagggagt cgcgttatcg cgagtaagga ttttggcttc gctttagata
      181 ttttcttttg tcgaatgatg gcaaacaatg tcagtcatat gtccgcgatt ttatatatag
      241 acaatcatac tttgtcagta aggctacgaa taaagcagtc agtgtatgga caattaaatt
      301 atgttgtgtc tgtttacgac ccgaacgata ccaacgttgc cgtcagaggc acccacaggg
      361 cagcacgggg ctttctctcg cttgataagt tcatcagttc agctcccgat gctcagacct
      421 gggctgatag gtatgttcgc aactgtgcaa ttgctattct tcctctatta cctgagggag
      481 ttccagttgc tattttagcg ggtatcacga cacgaatgcc atttgcccct atacatccat
      541 cggcaatgtt gttaataatg gccacaggcc agactcaata gcttattaca ctattcaaac
      601 agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag aatagcgttg
      661 gtacacctgc tttatttctg gctatgatga acggacaaac tgacaacgta aaaatattta
      721 tgcaagaaat tcagtcactg gtatacaatc atatcattca tgaagataat ctggttaaat
      781 tactgcaaac taaaagtgct aacgaaacac ctggactcta tatctccatg ttgtatggat
      841 tcgatgaaat aatcgatatc tttctgaatg cattaaccac tccaatagca caagaacttt
      901 taaacaaaaa aaatggtgat gagtatttta gccatgaaaa tacatgatgg tgagccagga
      961 ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct ctctaaaatt
     1021 aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt attaaaagga
     1081 gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa tgaagacgtc
     1141 gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt tagtcaacat
     1201 cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt tcatatagcc
     1261 attcatcata agcattataa aactgtagaa acatattatg ctgctataaa tgtcatcagc
     1321 caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc tatttacgat
     1381 atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac catgtgcaca
     1441 acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctatccctt ttgttacact
     1501 ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg ctttgataaa
     1561 aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt gttgctacgt
     1621 tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac cgcataacac
     1681 ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga cgatcggtat
     1741 gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc accttcagtc
     1801 caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt gtatcttccg
     1861 cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc catgcataag
     1921 ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat ttgtcgtaac
     1981 gcacacctgg gccgaattca aagcggaaac tgtgtaccgg gccattcaaa aactggcgac
     2041 cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct gtcagccagc
     2101 ttgcctgacc aaataaatag tcataatcag ttaaattaaa acggctacgc ccgcccgccg
     2161 catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc ccccacagcg
     2221 accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt gaggagcttt
     2281 tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa ggttttttag
     2341 cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca ttcagtgacg
     2401 caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc ttcatttatc
     2461 aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct taaggatcaa
     2521 agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact tagcaatgta
     2581 ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt atatatattt
     2641 atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt cactttccgc
     2701 tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt
     2761 tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa
     2821 aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg
     2881 ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg
     2941 aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag
     3001 actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag tatcgttttg
     3061 ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg
     3121 aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt gtgaagctgg
     3181 aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc atcgtcgaca
     3241 gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg gttaagccta
     3301 accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac gatgtccgca
     3361 aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc
     3421 cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca ccaccggtga
     3481 aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg
     3541 cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca
     3601 cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc
     3661 tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac ctgtcggcaa
     3721 cgcgtttctc cgactttgct caaaagtcat gtgataacaa aggggtgaac tatggccagt
     3781 ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt
     3841 aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag
     3901 ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca aagtgaagaa
     3961 gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatc
     4021 accatcacga cttgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa
     4081 tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct aaccttatga
     4141 tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag
     4201 cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc
     4261 gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga aagggaactg
     4321 cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc
     4381 accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat
     4441 tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg
     4501 cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa ctcgctctcc
     4561 acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc tgaacaacgg
     4621 attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc
     4681 atcgtcgagc atattcagca gcgtctggcc tcgcatcaac tgcagccttc tctgttgcac
     4741 ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct
     4801 gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca tactgaacag
     4861 ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa
     4921 cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt tggcggtcag
     4981 catttggtta ttgctcagca gtcattggat agattattag cagcatgata tgggttgagg
     5041 ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat
     5101 aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc aggaaaatag
     5161 tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt
     5221 tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac
     5281 tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc
     5341 agaacataac gttcacgcca ccattaaaat taggggtcgg ggagttatac cagaacagat
     5401 tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag tcgagcatga
     5461 ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca
     5521 atgtggagat caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa
     5581 tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta
     5641 gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat
     5701 cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc
     5761 ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct
     5821 ttacgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat ctcccgtcgt
     5881 aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc
     5941 cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc
     6001 atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc tgttgcgtta
     6061 tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa
     6121 aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc ggctgaaaaa
     6181 ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt
     6241 gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc ttctaaagca
     6301 gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta
     6361 ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa
     6421 tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca ttcaactgac
     6481 ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa
     6541 aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac
     6601 cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg
     6661 gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac
     6721 atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa accgatcgcc
     6781 cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca
     6841 accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct acaaggaatg
     6901 gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg tgttcccctg
     6961 ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa gggcaaccaa
     7021 ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc ccattcatta
     7081 cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca gatccagttt
     7141 catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct ttctggaata
     7201 agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta
     7261 tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta
     7321 attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccataaa
     7381 cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt
     7441 gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt
     7501 aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc
     7561 gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt
     7621 tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt cggtgtgctg
     7681 gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca
     7741 cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag cgttccgcag
     7801 ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc caatccgacg
     7861 tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt gaaactgctg
     7921 aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct acaaagctgg
     7981 gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct ggctctaatg
     8041 accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag tttcgttgtc
     8101 gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct gggcattaat
     8161 ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc
     8221 agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc
     8281 gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc
     8341 tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt tggcattaac
     8401 ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag ttccgcaggc
     8461 gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc tgcgatgggc
     8521 ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga catgggccgt
     8581 acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca gtggctgaag
     8641 caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca ccgttaattc
     8701 ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta aatgttccat
     8761 cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa tgtccaactt
     8821 atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa cctcataccc
     8881 gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca caccacttcc
     8941 cattcccgga agatttagtg gattaaaaag aagattgata ttaagcattg atattatcga
     9001 aggaaacttt aggagactct catttttttt gaatcgcagc aaaaatgctc ccggcaggat
     9061 gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac ctatttttta
     9121 tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc atcccaacaa
     9181 aattctcatt aatatctctt atccgaaact cactgccata aagattatta attatcactc
     9241 tatatttaac attgtcctgc agatacgtat caaaactaag tgctggagct cctggctttg
     9301 aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc gactctgcta
     9361 tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc atggtagact
     9421 caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca ccataattac
     9481 tctgtattaa gtttgtagaa aacatctccc gccccatatt gttaacaaaa ttattatctc
     9541 atttaaatct aagtcactta caatataagt ttaagagcga cgccacagga tgaactatca
     9601 aaaatagctc atcatgatta gcaaaactta accattttaa aataaataaa caattaaaga
     9661 aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt acaggtggtt
     9721 tgaatgaatg tagcaacgaa atgcagaatt tcaggtcatg taactcccgg caaaaccggg
     9781 aagtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt gcccaacgtt
     9841 gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc atcagtacaa
     9901 acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc tttatcgcat
     9961 gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg ttttgctggc
    10021 ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga aaaactgtag
    10081 tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca ccgtttccag
    10141 aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt ttgaccaaaa
    10201 cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca taacgaaaag
    10261 aacctacatc agcaccagtc accactacac gattccagcg aagcgaaacc ggggatggac
    10321 tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc gccaggtgca
    10381 caacctaccg cccctgggag cctgaaagcc cctgatacac gtaacgaaaa acttaattct
    10441 ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca gggcgtgcgc
    10501 atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct gctggaagat
    10561 tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga acgtattgtt
    10621 catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt aagcgatatc
    10681 accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt taggttctct
    10741 accgttcagg gtggcgctgg ctctgcagat accgtgcgtg atatccgtgg ttttgccacc
    10801 aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc aatcttcttt
    10861 atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga accgcactgg
    10921 gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc tctgcaacct
    10981 gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg cagttaccgc
    11041 accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg gaaggcaacg
    11101 tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg ggatgaagca
    11161 caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga agccattgaa
    11221 gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga agatgaattc
    11281 aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact ggtgcccgtt
    11341 cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc cgaaaacgaa
    11401 caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa cgatccgctg
    11461 ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg tgggccgaat
    11521 ttccatgaga ttccgattaa ccgtccgacc tgcccttacc acaacttcca gcgtgatggc
    11581 atgcatcgta tggggatcga cactaacccg gcgaattacg aaccgaactc gatcaacgat
    11641 aactggccgc gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc atatcaggaa
    11701 cgcgtggaag gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata ttattcccat
    11761 ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt cgatggtttc
    11821 agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt tgaccagctg
    11881 gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat cgaactgact
    11941 gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa ggatccatcc
    12001 ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc gattttactt
    12061 aatgatgaag tgagatcggc agaccttctg gccattctca aggccctgaa ggccaaaggc
    12121 gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga cggtacggtg
    12181 ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc ggtcattgtc
    12241 ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta cctgatggaa
    12301 gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt taaagcaaca
    12361 atcaaggtcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc tgacggtagt
    12421 tttatggatg aactgctaac gctgatggca gcacaccgcg tgtggtcacg cattcctaag
    12481 attgacaaaa ttcctgcctg atgggaggcg gcgcaattgc gccgcctcaa atgatttaca
    12541 tagtgcgctt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caaaactgtg
    12601 caaattcaat atattgcagg aaacacgtag gcctgataag cgaagcgcat caggcagttt
    12661 tgcgtttgtc agcagtctca agcggcgcag ttacgccgcc tttgtaggaa ttaatcgccg
    12721 gatgcaaggt tcacgccgca tctggcaaac atcctcactt acacatcccg ataactcccc
    12781 aaccgataac cacgctgagc gatagcacct ttcaacgacg ctgatgtcaa cacatccagc
    12841 tccgttaagc gtgggaaaca gtaagcactc tgacggatag tattatcgat aaacgccgga
    12901 tgacacatca cctccagcga acgatcaccc cgatggcttg cgtcatccag cacttgcagg
    12961 aacagtgcct cgctaatctc ttcaccataa aacgcgctac tgaatccctg agaactgcgc
    13021 agattagccg gtaaatcacc agagttagat agcggctgac ggtcgatgcg caacgcaatc
    13081 ccctcttcag ccgcaaacct ggcaacaatc gggaaaatct gcgggaacat atgcacatga
    13141 tgatggctat caagatgcgt aggtttgcgt ccaaatagct caatgaaacg caaatactga
    13201 ctggcaagct cctgagtaat ttcttccagc ggtaaagcat cttcttctgc caactgccag
    13261 atccattttc ccagcacacc atcgcgggtt aaccccggca tagctgtcag tggcttgccc
    13321 atagtaagga caaagtacat ccctatggcc agactcggtt catcacgact caactgcacc
    13381 gcatggtcaa tagcctgccc attcaccagc gccgtcgtcg atgtgacaat cccattgcga
    13441 caggcctcga taatgccgta gttctgacct ttgcttaagc caaaatcatc ggcattaaca
    13501 atcagtaagc gttccataat cagcctcggt taatgtgctt ttttaagctc tgcgatgcag
    13561 tcggcaaaat ttggcagcca tttctcgtgc gccagaatca tctcgcgtgc cagcagctca
    13621 gcatcgcgat cggaatgcac caacggacta aggtttagcg ccagtaacac atcgttaaat
    13681 tctccgctaa gtgcggcgtt gctggcagca atctcgaagc ctttaatggt gtgaatcagc
    13741 cccatcactt tatcatcgaa atgcgtaatg cgcggatgtg gcgtcgcgcc atcgcgcccc
    13801 agcttacagg tcatttctac cgcccagtct gccggaatat tatcaatatg cccatgatgc
    13861 gggatattaa cgtaatgttc agcttgcttg tcgttgtaga tagcgttgat cacttcgcac
    13921 gctgcatcag agtaataagc cccaccgcgc tgttccagtt ctttcggctt aactttcaac
    13981 tccgggtttt tatacagctc aaaaagttgt ttctcgactt tctgtactac ctgcgctcgt
    14041 gcgccgcctt tgtagtattc gcccatttca atagccagca tctctttttg cttgaagtaa
    14101 taaagcaaat aagaacacgg caacagattc agagaacgaa ttaagccttc gctaaatggc
    14161 agatcgaaaa tatttttaac gccagatgct tttaactgcc cggatgccac accatcaagc
    14221 aattcggcaa agcgcgactt gccatttacc agcacatcct taatgaacac catatggttg
    14281 aggccgaaca aatcgataga taaatcatcg ctgtctttca gcatcagaac atcgtgaata
    14341 aacatcttca tgccgatcgg aatattacac acgccgataa agcgtttaaa tccggtatga
    14401 cgataaacgg cttcagtaac cattcccgcc gggttagtga agttaatcac ccatgcattc
    14461 ggacaaagtt cttcgacatc ttttacgatg tcaaaaatca ccggaatggt acgcagacct
    14521 ttaaacagac cgcccgcgcc gttggtttcc tggccaagat aaccatgact taatggaata
    14581 cgttcatcca gttcgcgcgc cggtaattgg cctacgcgca gttgggtagt aacgaaatca
    14641 gcatctttca atgcttcgcg gcgatccagc gttttataaa gcttcatcgg gacgccagcg
    14701 ttatcaatca tccgttggca gagttcaaaa atgatatcca gtttctcttt accatcttcg
    14761 acatccacca gccataattc gctgaccggc aattcgtgat aacgcttaat aaatccttcc
    14821 agtaactccg gggtatagct gctcccgcca ccaatagtga cgacttttaa tttctggctc
    14881 ataatttctc ccttcagtac agaatactga tatctggcat atctgccccc ccggacataa
    14941 ataatccagc aacaggacag atatgtgaat tgtcaggtat aacgacttac tgattaaatt
    15001 cagtcaattt cttacgatag ctcttaggcg taaaggacgt taatttctta aacgttttaa
    15061 taaacaaact cgggctacta taaccggcct caaacgcaat atccgtcacg gaatagttgg
    15121 tcatttccag ttgttttttg gcaaaattaa tacggatctc attaataatc tgcattggtg
    15181 ttttgccata atatcgttga gtcgctcgcg tcaaatattc ctgtgatttg gctgacaacg
    15241 tcaccatatt ctccagcgcc gattcactaa actgctcttt atcatgcatc ttttctaccg
    15301 tacttttcag ccactgcggt acatcatcaa tcacctgttc ttcgcggtaa tgacgtaaac
    15361 ggttaatgac ataaaaagta accatctcaa caaactcttc cagccctgtt tcgcggaaat
    15421 tcaatgaaga aatcactgtt tccacatagg tgagaaaagc gttattggtc cggtatacct
    15481 gcgaagcgac aaagcaataa ggcaacaacg gcaggtaatg ctgctcaaaa aagcgtttac
    15541 tgatcccaac gttcaatata cgcgtggcac caaactcata aaaactttga tggtgcgaac
    15601 ctaacggaat aaaaacaaaa tcgccccgtt ccagtaacac gcgcttaccg ttaatctcct
    15661 ggaaataacg cccggttaat accagagtaa attcataata gtcgtgctga tgcagcccgc
    15721 tgatactctc agttttgtta tagataaaca catggaagtt tttgccatta aacaactgct
    15781 gttctcgggc agtggcaatt tccggcgcgt taatcactgg ctgcatcatc gactccttat
    15841 gccttcagtt tttcatgaag ctcaattaat tcagtaatca gttcacgcgc aagcatggac
    15901 gtcattaaat gatcctgagc gtggaccagc accagactca ctttcatctt accttcgccc
    15961 gcatcgcctt caatcagttt cgtctgtacc agatgcgctt cattcaatgc catccgtgac
    16021 tgatccatca tggcttttgc tgcagcaaaa tcgccctgct tcgcctgttt cagtgccgca
    16081 tacgccaggc tgcgcgcttg tccggagttg atgataagcc ccatcaccac ttcttccagc
    16141 tcttcagctt ccgtttgcgt atcgggaatg ttatcgagat ccatcataca tcgttcctct
    16201 ctattcttac cggcacgatt acccgtaccg gcatcgatta aaatttcagg gcgttagcga
    16261 tatcttcttc gcgctcttct ttatcaatcg cgttttgtgc tttgttagcc accacaacaa
    16321 agggcagata aattaacgtt gcgataccaa ggttgaagag tgcaaccagc aatgcggcga
    16381 cgctaccgtt ggtgttaaag aaggctccca gaccggttgg catggtccac ggtgcaatat
    16441 tggtcaccgg aggaatgatg cccatgtagt acgctgcgag ggtgattgcc gccagaatcg
    16501 gttgtaccag tacaaacggg ataaacatca ccgggttcat gataattggc agaccaaaca
    16561 gaatcggttc gttaatctgg aagatgccgg acggcagcgc cagttttgcc acctgacgat
    16621 aatcagcacg gcgagaggcg ataaagatag ccaggatcag gcctaaagtc gcaccactgc
    16681 ccccaaggaa aataaaggag tccagcatcg gcttggccca gatatggaag gtcttaccgg
    16741 ctgccagcgc cgcttcgacg gaaccatatt gctgataggt cgcgatattt tccagcgccc
    16801 acggcgtcat aatgccgttg tccagtgcgg tcagcgccag cgcgccatga ataccgaaga
    16861 accagagcag tggaacaaag atcacatagg cccagcccac cacgctaccc aacgatgcca
    16921 gtggggttga gatggtatcc ataatgatct gatggaagtt ggtgccccag gtattcaacg
    16981 cccaggcaat aatccccatc acggaaagaa taataaagcc gggaattaat gccgagaagg
    17041 aacgcgatac tgaagcaggt acgctatcgg gcaatttaat gacccaattg cggcggacaa
    17101 taaaggtaaa catttctgcc accaccaggc caataataat cccggagatg atattcgctc
    17161 cacctaacca gtttgcgcca accacatagg cctcaccgac actatatggg gtgacggtca
    17221 taaatgctgc aacggataac aacccagccg ccagcgcatc gactttacgc tcttctgcca
    17281 gcgccatgcc aataaagaac ggtgccatta aagacattat ccctaatgtt ccgttatata
    17341 cgttgccgcc aatacctttc aggccattaa gtgtttcaat ggttgaggcg tcgaggcgaa
    17401 tacctaagga ataaaaaaac gccccctccc caaagcttag aaaaacgttg ttaattaata
    17461 caaacatggc ccccgcaagg gttaacggca ttaagcgaat aaagccattt ttgattgcat
    17521 taacgtgtgg ctgctttcct attttaactg caaaagggag gagtaccttt tcaagcgatg
    17581 caataacatt actcatagaa aaataccctt aaaaaccgca atttaaatat tgcggtattg
    17641 atttatgaaa taactctttg acgggaaaat ttaaaataaa ttaatttgct gcggcttttt
    17701 taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc gactttgcca taaagcagcg
    17761 agtcaattac ttcaaccggt ttgttgggta acaaacgctg gatttcgggc aacatataag
    17821 caatctgcgg ccctaataac acgacatcgg cattctgacc tttttcacca gccagtgttt
    17881 ccggaaatgc ttcaataatg accggaactt catatttttc tgcctgtgcg cgcatttttg
    17941 ataccagtaa agaggtagac atgcccgcag aacaaaacag ataaatgtgt ttcttttcca
    18001 taaaactgcc ctcatcgacg attatctgtc agccagacac tccgcaagcc ttaacctgct
    18061 tccatgctct gggtaacttg cgaaaccaac atgacttttt ttgtggctga aaggagtata
    18121 cggtatagac ctgagcaata gtatttcttt gaccttataa attgtttctc cttgacctgt
    18181 ggttatgacc ctcttcacat ttcgggcaaa taattcgcga agataattaa gaactcaggc
    18241 caaaaaaaac cggcgcaatg gccggtttcc gttgttactc aagctttcag acgaattgat
    18301 tacttcgcag cctgtggatc agtgtcgtat tcagcacagg tctgataacc ggagttgatg
    18361 acatgtccgg tatcatctaa cgcgacaaag taggtttctg ctttaccatc acgttgaccc
    18421 aggatgtagg tctggcaagt accacgagca tggatcatgc tcacttcaga cgaaggttta
    18481 cccgcaattt gtgcaacctg cgcccggctc atgccttttt tgacgtcttt caccacaggc
    18541 tgtacaaact ggtctttagt acgatcataa gccgtacaac ccgccagcat ggttaatacc
    18601 gccgctgcac tcagaattcc tgccatattc ttgttcatat tccgtcctct tgtttatcag
    18661 cgtgttagat aagcctggaa tacattgggc gctttttcaa gcccgcgaac gaaacggctc
    18721 cgctttcaga ggattcctgt atgacgtttt aaccaccatt cagcccgctg tcgcttgtcg
    18781 tttcagtggc aacgggttag ctttaaggaa gttttgtctt ttctgtctgg aggggttcaa
    18841 tgacattgca acaacaaata ataaaggcgc tgggcgcaaa accgcagatt aatgctgaag
    18901 aggaaattcg tcgtagtatc gattttctga aaagctacct gcaaacttat ccgttcatta
    18961 aatcactggt gctcgggatc agcggcggtc aggactccac gcttgccgga aagctgtgcc
    19021 agatggcgat taatgagctg cgcctggaaa ccggcaacga atcactgcaa tttattgccg
    19081 tacgcctgcc ctatggtgtt caggccgacg aacaagattg ccaggatgcc attgccttta
    19141 ttcaaccgga tcgcgtatta accgttaata tcaagggcgc ggtattggcc agcgaacagg
    19201 cattgcggga agcaggcatt gaactgagcg attttgtccg tggcaatgaa aaagcgcgtg
    19261 agcggatgaa agcacaatat agcattgcgg gtatgaccag cggtgtcgtg gtgggcaccg
    19321 atcatgcagc agaagccatt accggattct tcactaaata tggtgacggc ggtacagaca
    19381 ttaatccgct gtatcgtctc aacaaacgtc agggtaaaca gttactggcg gcattaggtt
    19441 gcccggaaca cctttataag aaagcgccaa cggccgatct ggaagatgat cgcccttctc
    19501 tgccagatga agtggcactc ggcgtgacct atgacaatat cgacgactat ctggaaggga
    19561 aaaacgtacc tgaacaggtc gccagaacaa tagagaactg gtatctgaaa accgaacata
    19621 aacgccgtcc gccaattacc gttttcgatg atttctggaa aaagtaataa tttgcacata
    19681 atggtttgtg cgaaaaagag taatttgttc acgccggatg cggcgtgaac gccttattcg
    19741 acctacaaaa ctatgcaaat tcaatatatt gcaggagtcg aataggcctg acaggcgtag
    19801 cacgtcagac ggtgtaacct ttgtcatcga cccgcctctt ttttaatcgc tccccgcctg
    19861 ttacactgga tagataacca gcattcggag tcaacagtgg tacggcgttt aacttctccg
    19921 cggctcgaat ttgaagctgc ggcaatttat gaatatcccg aacatttacg ttcattcctt
    19981 aatgacttac ccacccgacc aggggtgtat ctgtttcatg gcgaaagtga caccatgccg
    20041 ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc tttctcattt acgcaccccg
    20101 gatgaagccg ccatgctacg gcaatcccgg cgaattagct ggatctgtac cgcaggcgaa
    20161 atcggcgctc tgctccttga agcgcgatta atcaaagaac aacagccgct gtttaataaa
    20221 cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa atgaaaagcg cgtcgatgtg
    20281 gtgtatgcca aagaggtgga tttttcacga gcccccaacc tgtttggcct gtttgccaat
    20341 aggcgcgcag ctttgcaagc attgcagacc atcgctgatg aacaaaaact ttgttatggc
    20401 ctgctgggac tggaaccgtt aagtcgcggt cgtgcatgtt ttcgttcagc gctaaaacgt
    20461 tgcgccggag catgctgcgg taaagagagc catgaggaac atgcgctacg cttgcgccag
    20521 tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag gtgcggtggc actgaaagaa
    20581 cagcacccgg aaatgactca atatcatatt attcaaaact ggctgtggct gggggcggtt
    20641 aattcgctgg aagaagcgac aacgttaatt cggacacccg ccgggtttga tcacgacggt
    20701 tataaaattc tttgtaagcc gctgctttcc ggtaactatg aaattactga acttgatccg
    20761 gcgaatgacc agcgagccag ttgatttcac tgaacaacag tttaccttgc ggttgtaaaa
    20821 gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg gtcggtagtg agcaatttat
    20881 cgcccagttg ccacgccccg ttaatcacaa acaccacccc gccgcgtgag ccaaaggtag
    20941 taaacgtacg ttcggcaatt ctgactttcg ccttacagac atccaggcgt gtcatgatgt
    21001 tgaaatccat cgacatctgc ccttccgtca gtttcgcttt taccacctgg tccgctgcaa
    21061 aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg gtctgcgctt tcaaggagca
    21121 tctcgccgcc ttccagcaac gtcactatcc tttccatgcc gggaaacaaa gaaaactccc
    21181 cattcgccgc gatggatgca atgctggcac gccagtaaaa atcacgtttt gccggtggga
    21241 aagtgcaaat ttcgcgcgtt tcaccggcag catttcgcca cagattcacc gacattttac
    21301 gcatatcaaa gtattccata ctcgctcccg gcccgttctt ttcattgtta tcgttcaggt
    21361 aaccgtggat ggttatcggc aactcgggtg atttacgtta gtggtgatca ggcttttttt
    21421 acaactgttt gagaagagaa aagaaaaccg ccgatcctgt ccaccgcatt actgcaaggt
    21481 agtggacaag accggcggtc ttaagttttt tggctgaaag attattcagc agttgcaggc
    21541 attttacctt ttg
//
LOCUS       DAJTVK010000068        21357 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000068 DAJTVK010000000
VERSION     DAJTVK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.068"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(233..778)
                     /gene="hemG"
                     /locus_tag="OO372_002552"
     CDS             complement(233..778)
                     /gene="hemG"
                     /locus_tag="OO372_002552"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6342016.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(790..2241)
                     /gene="trkH"
                     /locus_tag="OO372_002553"
     CDS             complement(790..2241)
                     /gene="trkH"
                     /locus_tag="OO372_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6342017.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2280..2894)
                     /locus_tag="OO372_002554"
     CDS             complement(2280..2894)
                     /locus_tag="OO372_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6342018.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2894..4225)
                     /gene="pepQ"
                     /locus_tag="OO372_002555"
     CDS             complement(2894..4225)
                     /gene="pepQ"
                     /locus_tag="OO372_002555"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6342019.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVELLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4415..6604
                     /gene="fadB"
                     /locus_tag="OO372_002556"
     CDS             4415..6604
                     /gene="fadB"
                     /locus_tag="OO372_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6342020.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6614..7777
                     /gene="fadA"
                     /locus_tag="OO372_002557"
     CDS             6614..7777
                     /gene="fadA"
                     /locus_tag="OO372_002557"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6342021.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7967..8668)
                     /gene="fre"
                     /locus_tag="OO372_002558"
     CDS             complement(7967..8668)
                     /gene="fre"
                     /locus_tag="OO372_002558"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6342022.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8715..10208)
                     /gene="ubiD"
                     /locus_tag="OO372_002559"
     CDS             complement(8715..10208)
                     /gene="ubiD"
                     /locus_tag="OO372_002559"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6342023.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     TGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10375..10863
                     /gene="rfaH"
                     /locus_tag="OO372_002560"
     CDS             10375..10863
                     /gene="rfaH"
                     /locus_tag="OO372_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6342024.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10860..11642)
                     /gene="tatD"
                     /locus_tag="OO372_002561"
     CDS             complement(10860..11642)
                     /gene="tatD"
                     /locus_tag="OO372_002561"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6342025.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11684..12460)
                     /gene="tatC"
                     /locus_tag="OO372_002562"
     CDS             complement(11684..12460)
                     /gene="tatC"
                     /locus_tag="OO372_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6342026.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12463..12978)
                     /gene="tatB"
                     /locus_tag="OO372_002563"
     CDS             complement(12463..12978)
                     /gene="tatB"
                     /locus_tag="OO372_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6342027.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12982..13251)
                     /gene="tatA"
                     /locus_tag="OO372_002564"
     CDS             complement(12982..13251)
                     /gene="tatA"
                     /locus_tag="OO372_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6342028.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13330..14970)
                     /gene="ubiB"
                     /locus_tag="OO372_002565"
     CDS             complement(13330..14970)
                     /gene="ubiB"
                     /locus_tag="OO372_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6342029.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14967..15572)
                     /gene="ubiJ"
                     /locus_tag="OO372_002566"
     CDS             complement(14967..15572)
                     /gene="ubiJ"
                     /locus_tag="OO372_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6342030.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15586..16341)
                     /gene="ubiE"
                     /locus_tag="OO372_002567"
     CDS             complement(15586..16341)
                     /gene="ubiE"
                     /locus_tag="OO372_002567"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6342031.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16436..17863)
                     /gene="rmuC"
                     /locus_tag="OO372_002568"
     CDS             complement(16436..17863)
                     /gene="rmuC"
                     /locus_tag="OO372_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6342032.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18004..18765)
                     /gene="udp"
                     /locus_tag="OO372_002569"
     CDS             complement(18004..18765)
                     /gene="udp"
                     /locus_tag="OO372_002569"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6342033.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19027..19839
                     /locus_tag="OO372_002570"
     CDS             19027..19839
                     /locus_tag="OO372_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6342034.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20233..21294
                     /gene="hcp"
                     /locus_tag="OO372_002571"
                     /pseudo
     CDS             20233..21294
                     /gene="hcp"
                     /locus_tag="OO372_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5239 a         5719 c         5357 g         5042 t
ORIGIN      
        1 tgccgcttcg ctttttctca gcggcgcggg gtgtgcataa tacgctttcc cgctacagag
       61 tcaagcattt atttttgctt tctctgtcgg aattctcagg agaaccccgc tgacccggcg
      121 gcgtgtttgc cgttgttccg tgtcagtggt ggcgcattat agggagttat tccggcctga
      181 caagaggaaa tttaaaataa ttttctgacc gcgcattttt tattctttat gcttatttca
      241 gcgtcggttt gttggttaac tgggcgattt ctcgggcgaa attcgccacc tgctcccaat
      301 cggtatagac aacttctttg cgcgtatccg tttcaccgcc tgacatcttc ataattagct
      361 tgatcataaa acgatcgtac cagcgataac gtgggtaacg cagcgccccg gcaatgaccg
      421 cgcagcgatc gggacgccat tgcgagttca tcagaaactt ccgcgcgtag ctgttggtct
      481 gtggagtacg cttctccggt ttgcgcgcca ccaggtttac ggaataaaag gcgctcggca
      541 tcgaattcag tcgcgtcgca tgttttttga caaattcctg gaacgcagag tggtagtgac
      601 catagcgaat agaagcacca atgaccacac ggtcataatt ttcccattgt ggttcttcaa
      661 tgcggtgcac attggcgaca tccgtctgga tccccagttc tttcagttcc gaagccaggt
      721 aggaggcaat ctcgcgcgtt tgtccgtccc ttgttgagaa aagaattaat gttttcacgt
      781 attactccat cattcacgcc agaaagtcgg ggtaaagagc accagcaatg taaagacctc
      841 gagacgacca aacagcatgt tggcaatcag gatccattta gccaccgggt tcatactggt
      901 aaagttatca gcaaccacgc caagccctgg ccccaggtta ttcaatgtcg caacaactga
      961 agcaaaggca gaaaagtcat ccacgcccgt ggcgataatc gccagcatac tgacaataaa
     1021 caccaatgca taggcggaga aaaatcccca aacggcttcg aggatacgtt ccggcagtgc
     1081 gcgattcccc agcttaatgc tatagacggc gttcggatgc accagtcgtt tcagctcacg
     1141 gttcccctgc ttaaacagca gcaggatgcg gatcactttc aggccaccgc ccgttgaccc
     1201 ggcgcaaccg ccgataaaag ctgaacataa aagcagtacc ggcaaaaaga gcggccagcg
     1261 ggcaatgctg tcagtcgtaa acccggcggt tgtcgccatc gataccacct ggaaaaacgc
     1321 ctggttaatt gtcatcagcg ccgaactgta gacattatga aaccacagta cgagtgtaca
     1381 aataaccacc agcgtaaact gtacgccgat aaacatgcga aattccggat cgcgccaata
     1441 aaccttcaga ctacgcccac ttaacagtga aaagtgcaga ccgtagttac agccggagat
     1501 cagcaggaag atagcaatga tagtgttaat agtcgggctg tcgaaataac cgatactggc
     1561 atcatgtgtc gagaagccgc caatagcgat agtcgcaaag ctatggccga tggcatcaaa
     1621 ggcatccatt ccggcaaacc acaacgccag cgcacaggcg acggtcagca agacataaat
     1681 caaccacagg gttttcgccg tttccgcaat tcgcgggcgc attttgttat ctttcagcgg
     1741 gccgggcatt tctgcgcgat agagctgcat cccacccacg ccgaggatag gcagtatcgc
     1801 aaccgctaac acgatgatcc ccatcccgcc aaaccattgc agcatctggc gataaaagag
     1861 gatggcatga gggagcgaat ccagccccac cagcgtagtg gccccggtgg tggtcaggcc
     1921 agagaaagat tcaaaaaacg catcggtaat cgtgaggttc gggctttccg agaagataaa
     1981 agggagcgca ccgacactac ccagcacggt ccagaacagc accactatca gaaacccctc
     2041 acgggatttc agttcgccct tctctttacg gttcggccac cacagcatag aaccaatggc
     2101 gagggcgaca aaaaaggtct gggtaaaagc gcggcccgct ccatcccggt agatgagtgc
     2161 taccagccca gggataatca tggtccctga aaataagatg accagtagtc caacgattcg
     2221 ggtaatggcg cgaaaatgca tctctgccgc ttccttagtt ctgcaaaacg aagtggggat
     2281 tattcttcaa tcgctaacaa ttgcaatgaa ccacggctaa aatccgccag ctttgcggaa
     2341 aattcagcca ctttcgccgc cggaagcgcc acccgcagca gaacgaatgc ctgataatca
     2401 ctgttgataa ttttgccgtc acactgcccc agcaacgctt caatgccggt taactgatga
     2461 tattcacatt gcaaagtata ttcggttaat ggcgtcttgc gttgggtcgt tagctggcgc
     2521 agcgcctgat tcacgccgcc gccatacgct ttcactaacc caccggtgcc aagcaatatg
     2581 ccgccgtagt agcgcactac cacagaggta atttccccga cgccgctgcc cattagctgg
     2641 gcgagcatcg gtttacctgc cgttcctgcc ggctccccgt cgtcagagaa acccagctgt
     2701 tgagaatcat ccggcgcacc cgccacccac gccacgcaat ggtggcgggc atcggggtgt
     2761 tctgcccgca ccgattcaac aaacgctttc gccgcctcaa cgccatcggt atgcgccaac
     2821 atcgttatga agcggctttt tttgatctct tcaacgaccg tgaccggtgc cgcaggaatt
     2881 aaccagcttt ccatcacgcc agtttcagat cccgggtcat gttttccacg ttgttttcgt
     2941 ggatcaccac gttgtcttcg atacgaatgc cgccgaacgg tttcagtgct tcaattttct
     3001 gccagttgaa gtgcttgctg aactgccctt cacgccacgg tgccagtagc gattcaatga
     3061 agtagatacc cggttcgatg gttaacacca tacccggctg gagaatacgg gtgcagcgca
     3121 ggtacggata ttttgccggt gccgcgaggt gtgtaccgct atcatcctgc ataaaaccgg
     3181 ctacgtcatg cacctgcagg cccagcggat gaccgatacc atgcggcata aacggtccgg
     3241 tgagatcgtt ttcgaccatc gcttcttcac tcatatcggt gatgatttga tgtttacgca
     3301 gcagtttggc gatgcgctga tggaactgga tgtggtaatc cacatagcta acgcctgctt
     3361 tcatggtggc gatcagcgcc agttgttcat catttacgtc tttcaccagc tgtgcgtagt
     3421 cgttgtcgct ttttgccgac caggtacgag tcaggtcagc cgcatagccg ttatattcgg
     3481 ccccggcatc cagcaggaag ctgcgcatct cttccggtgc ctgatgatcc agtttggtgt
     3541 aatgcagcac cgaagcgtgt tcgttaagcg ccacaatgtt gctgtaaggt acgtcggtat
     3601 cacgatgacc ggtcgcggtc agataggcga tgttgatatc gaactcgctc atgccagaac
     3661 ggaacgcttc ttctgccgca cgatgaccgt tgaccgccat tttctgcgct tcacgcatac
     3721 aggccagttc gtactcggtt ttgaaggagc ggtagtaatg caggtagtcg atcacccctt
     3781 tcgggttgat gttgctggcc tcaataccca gttgcagcgc acgttccggc accggaccga
     3841 tataaccgat attgccgcgc gcagcgggca gcaggctacc aatgccatcg gctttcggca
     3901 gtgcgatcac ttccacatct tcagtccaga aggaggtcgg cagcagttcg acgttgtgcc
     3961 agtaatcaac cggcagatag aaccacagtt tcggcttgtt cacgccatcc accagcaacc
     4021 agcagtttgg cacctgagtt accggcaccc acgctttgaa ttgcgggttc actttaaacg
     4081 gatagggatg atcgtcgaga aaaacgttga acagctcgcc ggagtgaata agtaatgcat
     4141 ccagcttgaa gcgcgccagc gcatcgcgag tccgttcttg taaggtagct atatgatttt
     4201 tatagagcga ggccagtgat tccatttttt acccttctgt ttttttgacc ttaagtctcc
     4261 gcatcttagc acatcgttca tccagagcgt gatttctgcc gagcgtgatc agatcggcat
     4321 ttctttaatc ttttatttgc atatttttaa cacaaaatac acacttcgat gcatctggta
     4381 cgaccagatc accttgcgga ttcaggagac tgacatgctt tacaaaggcg acaccctgta
     4441 ccttgactgg ctggaagatg gcattgccga actggtattt gatgccccag gttcagttaa
     4501 taaactcgac actgcgaccg tcgccagcct cggcgaggcc atcggcgtgc tggaacagca
     4561 atcagatcta aaagggctgc tgctgcgttc gaacaaagca gcctttatcg tcggtgctga
     4621 tatcaccgaa tttttgtccc tgttcctcgt tcctgaagaa cagttaagtc agtggctgca
     4681 ctttgccaat agcgtgttta atcgcctgga agatctgccg gtgccgacca ttgctgccgt
     4741 caacggctat gcgctgggtg gtggctgcga atgcgtgctg gcgaccgatt atcgtctggc
     4801 gacgccggat ctgcgcatcg gtctgccgga aaccaaactg ggcatcatgc cgggctttgg
     4861 cggttctgta cgtatgccac gtatgctggg cgctgacagt gcgctggaaa tcattgccgc
     4921 cggtaaagat gtcggcgcgg atcaggcgct gaaaatcggt ctggtggatg gggtagtcaa
     4981 agcagaaaaa ctggttgaag gcgcaaaggc ggttttacgc caggccatta acggcgacct
     5041 cgactggaaa gcgaaacgcc agccgaagct ggaaccgcta aaactgagca agattgaagc
     5101 caccatgagc ttcaccatcg ctaaaggaat ggtcgcgcaa acggcgggga aacattatcc
     5161 ggcccccatc accgcagtaa aaaccattga agctgcggcc cgttttggtc gtgaagaagc
     5221 cttaaatctg gaaaacaaaa gttttgtccc gctggcgcat accaacgaag ctcgcgcact
     5281 ggtcggcatt ttccttaacg atcaatatgt aaaaggcaaa gcgaagaaac tcaccaaaga
     5341 cgttgaaacc ccgaaacagg ctgctgtgct gggcgcaggc atcatgggtg gcggtatcgc
     5401 ttaccagtcc gcgtggaaag gcgtgccggt tgtcatgaaa gatatcaacg acaagtcgtt
     5461 aaccctcggc atgaccgaag ccgcgaaact gctgaacaag cagcttgagc gcggcaagat
     5521 cgatggtctg aaactggctg gcgtgatctc cacgatccac ccaacgctcg actacgccgg
     5581 atttgaccgc gtggatattg tggtagaagc ggttgttgaa aacccgaaag tgaaaaaagc
     5641 cgtactggca gaaaccgaac aaaaagtacg ccaggatacc gtgctggcgt ctaacacttc
     5701 aaccattcct atcagcgaac tggccaacgc gctggaacgc ccggaaaact tctgcgggat
     5761 gcacttcttt aacccggtcc accgaatgcc gttggtagaa attattcgcg gcgagaaaag
     5821 ctccgacgaa accatcgcga aagttgtcgc ctgggcgagc aagatgggca agacgccgat
     5881 tgtggttaac gactgccccg gcttctttgt taaccgcgtg ctgttcccgt atttcgccgg
     5941 tttcagccag ctgctgcgcg acggcgcgga tttccgcaag atcgacaaag tgatggaaaa
     6001 acagtttggc tggccgatgg gcccggcata tctgctggac gttgtgggca ttgataccgc
     6061 gcatcacgct caggctgtca tggcagcagg cttcccgcag cggatgcaaa aagattaccg
     6121 cgatgccatc gacgcgctgt ttgatgccaa ccgctttggt cagaagaacg gcctcggttt
     6181 ctggcgttat aaagaagaca gcaaaggtaa gccgaagaaa gaagaagacg ccgccgttga
     6241 agacctgctg gcagaagtga gccagccgaa gcgcgatttc agcgaagaag agattatcgc
     6301 ccgcatgatg atcccgatgg tcaacgaagt ggtgcgctgt ctggaagagg gcattatcgc
     6361 cactccggcg gaagcggata tggcgctggt ctacggcctg ggcttccctc cgttccacgg
     6421 cggcgcgttc cgctggctgg acaccctcgg tagcgcaaaa tacctcgata tggcacagca
     6481 atatcagcac ctcggcccgc tgtatgaagt gccggaaggt ctgcgtaata aagcgcgtca
     6541 taacgaaccg tactatcctc cggttgagcc agcccgtccg gttggcgacc tgaaaacggc
     6601 ttaaggagtc acaatggaac aggttgtcat tgtcgatgca attcgcaccc cgatgggccg
     6661 ttcgaagggc ggtgcttttc gtaacgtgcg tgcagaagat ctctccgctc atttaatgcg
     6721 tagcctgctg gcgcgtaacc cggcgctgga agcggcggcc ctcgacgata tttactgggg
     6781 ttgtgtgcag cagacgctgg agcagggttt taatatcgcc cgtaacgcgg cgctgctggc
     6841 agaagtgccg cactctgtcc cggcggttac cgtcaatcgc ttgtgtggtt catccatgca
     6901 ggcactgcat gacgcagcac gaatgatcat gactggcgat gcgcaggcag gtctggttgg
     6961 cggcgtggag catatgggcc atgtgccgat gagtcacggc gtcgattttc accccggcct
     7021 gagccgcaat gtcgccaaag cggcgggcat gatgggctta acggcagaaa tgctggcgcg
     7081 tatgcacggt atcagccgtg aaatgcagga tgcctttgcc gcgcggtcac acgcccgcgc
     7141 ctgggccgcc acgcagtcgg gcgcatttaa aaatgaaatc atcccgaccg gtggtcacga
     7201 tgccgacggc gtcctgaagc agtttaatta cgacgaagtg attcgcccgg aaaccaccgt
     7261 ggaagccctc gccacgctgc gtccggcgtt tgatccagta agcggtacgg taacggcggg
     7321 tacatcttct gcactttctg atggtgccgc ggccatgctg gtgatgagtg aaagccgcgc
     7381 ccgtgaatta ggtcttaagc cgcgcgcccg tgtgcgttcg atggcggtcg ttggttgtga
     7441 tccgtcgatt atgggttacg gcccggttcc tgcctcgaag ctggcgctga aaaaagcggg
     7501 gctttctgcc agcgatatcg gcgtgtttga gatgaacgaa gcctttgccg cgcagatcct
     7561 gccgtgcatt aaagatctcg ggctaatgga gcagattgac gagaagatca acctcaatgg
     7621 tggtgcgatc gcgctgggtc atccgctggg ttgttccggt gcgcgtatca gcaccacgtt
     7681 gctgaatctg atggaacaca aagacgttca gtttggtctg gcgacgatgt gtatcggtct
     7741 gggtcagggt attgcgacgg tgtttgagcg ggtttaacca cttgccggat gcggcgtgaa
     7801 cgccttatcc ggtctaccga tcctgcacca atgtaggcct gataagacgc gccagcgtcg
     7861 catcaggcat cgtgcaccga ttgccggatg cgacatagat ctaaatgcca ctgaataaag
     7921 ttttagttgc cgttcttccc gcctgtcagg ggcgggtttt tatatctcag ataaatgcaa
     7981 acgcatcgcc aaacagacga tcttcccgcg cattacgctc actgcaaaac aggtcacgag
     8041 caattttcgc catctcaaaa cgtccggcaa tatagatatc atgctctgcc agcgtaccat
     8101 gatcctgcaa gaccgccgtt aacacggtgc cagtacgccc acgccagccc gcttccggtt
     8161 gttcaaccac cggcaccact tgcagaccag gatgcttcag cgaaagcgcc tcaagctcgc
     8221 agagatcata cagatgttgc tcttcacgcc caccccagta aatggtgata tcgcgattgg
     8281 gattacgcgc caacgccgtc aataaaatcg agcgcgcata ggagaacccg gtgccgcccg
     8341 caatcaaaat catcggacgc tcttcatcat cgcgcagcca cgcttctccg tggggaatat
     8401 cgaccacgat ttgatgatct ttgaggatgc ggtccatgac tgctttcgcg taaaggttga
     8461 tttcagaagc gccaatatgc agctcgataa accctttttc atccggcgtc gaagccattg
     8521 aaaacggacg tttgtcgcgc tcatccatca ctaccatcaa atactgacca gcacgaaaag
     8581 aaaaggccgc gtctggtacg attcggacac ggtatacagt atccgtgata gcttctaccg
     8641 aggtcacttt acagcttaag gttgtcatgc gctttctctg tcggatcaaa taaatagggc
     8701 aaaactaacg cgcatcaggc gcttttaccg ttgttaaaaa tagccagttc atcccagatg
     8761 gcgtcaatat gcgcgacgac atcaggatct tttttgatgg gacgtcccca ttcacgctgg
     8821 gtttcccccg gccatttatt cgtggcatcc agccccattt ttgaacccag cccggagaca
     8881 ggcgaggcaa aatccagata atcaataggc gtattttcta ccagaacagt atcccgcgcc
     8941 gggtccatac gggtggtaat cgcccaaatc acatcgttcc agtcgcgtgc gttgacgtca
     9001 tcatcgcaaa cgatcacaaa tttagtgtac ataaactggc gtaagaacga ccagacgccc
     9061 atcatgacgc gcttcgcgtg tccggcgtac tgttttttga ttgtcactac cgccagacga
     9121 taagaacagc cttccggtgg caggtaaaaa tcgacaattt ccgggaactg tttttgcaga
     9181 atcggcacaa acacttcgtt cagtgcgaca cccagcaccg cgggttcatc cggcggacga
     9241 ccagtatagg tggagtggta aatcgcatct tcacgctggg taatatgcgt cacggtaaat
     9301 accgggaaac tatcgacttc gttatagtaa ccggtgtggt cgccatacgg cccttccggc
     9361 gcagtttcgc cttgttcgat atacccttcc agcacaatct ccgcactggc gggcacttca
     9421 aggtcattgg agatacactt caccacttcg gtcttggtac cgcgcagcaa tccggcaaac
     9481 gcatactctg aaagtgtatc cggaacgggg gtgactgcac cgagaatcgt ggcgggatcg
     9541 gcacccagcg ccacagaaac cgggaaacgt tcgcccggat gcgccgcaca ccactcctga
     9601 taatccagcg cgccgccgcg atgcgacagc cagcgcataa tcagtttgtt tttaccaatc
     9661 agctgctggc gataaatgcc cagattctgc cgctctttat gcgggccgcg cgttacggtc
     9721 agcccccagg taatcagcgg cgcggcatct tccggccagc aggtcataat gggaatgcga
     9781 ttgagatcga cgtcatcgcc agagacgatt ttttgttggc agggcgcacc acgcagccgc
     9841 tttgtcggca tgttcaatac ttgcttaaac tgcggcagtt tatcaaacag gtcgcggaaa
     9901 ccttttggcg gctcgggctc tttcagaaac gccaataatt taccaacttc acgcagcgcc
     9961 gaaacatctt cctgccccat gcccatcgcc acgcgctttg gcgtaccgaa caggttgcac
    10021 agcaccggca ttgagtagcc tttagggttt tcgaacaaca gcgcaggccc accggtacgc
    10081 aaagtgcggt cagcaatttc agtgatttcc agatgcggat ccaccgggag cgtgatacgt
    10141 tttagctcac cctgctgttc aagcagcgtc aagaagtcgc gtaaatcgtt atatttcatg
    10201 gcgtccattg tagcctctta atctacgccc attatacggc gttcatcttt gcgatgctgt
    10261 aaatttgtta aattagcgtg aactctgacg gtataacgca aaccggggaa tataattaac
    10321 ttagcgtaaa gcttttgcta tccttgcgcc ccgattaaac ggataagagt cattatgcaa
    10381 tcttggtatt tactgtactg caagcgcggg caacttcaac gtgcccagga acaccttgag
    10441 agacaggctg tgaattgcct ggcaccgatg atcaccctgg aaaaaatcgt gcgtggaaaa
    10501 cgtactgcag tcagtgagcc attgtttccc aactacctgt ttgtcgaatt tgatccagaa
    10561 gtgattcata ccacgactat caacgcgacc cgcggtgtca gccacttcgt gcgctttggc
    10621 gcgtcgctag cgatagtccc atcggcggtg attcatcagc tatcggtata taaaccgaaa
    10681 gacattgtcg atccggcaac cccttatccg ggagataagg tgattattac cgaaggcgcg
    10741 ttcgaaggct ttcaggccat tttcaccgaa cccgatggtg aggctcgctc catgctattg
    10801 cttaatctta ttaataaaga gattaagcac agtgtgaaga acaccgagtt ccgcaaactc
    10861 taaaacgcaa tcccaaaccg tgttttgaca ttagcatccg tggtggcagc cagccatgcg
    10921 gcatcttctc cacgccagtg cgcaatacgt tgcaaaatat ggggcagatg ggctggctcg
    10981 ttgcgccggg atgatggctt tggcgtgaga tcgcgaggga gcagatacgg cgcatcagtt
    11041 tcgatcagta atttttccgc cggaatcaac ggcaaaagtt cccgcagctc cagtccgcgt
    11101 cgttcatcgt aaacccaacc ggtaatgccg atataaattc catgcgccac gcacgcctgc
    11161 atctcttcgc gtgtgccggt aaagcaatga agaaccgcac caggcagttt atccagccac
    11221 ggctccagca atgtcataaa ccgctcgtgg gcatcgcgac agtgcataaa taccggcatg
    11281 tttaaatctg cggcaatgcg tagctgggca acaaaagcgc gttcctgctc ttccggcgtc
    11341 gaaaagttgc ggttaaagtc gagaccacat tcaccaatcg ccaccacttc tggctgcgcg
    11401 gccagctcaa taatcgcttc ttcagtcgca gcttgccact ggctgctgtc gtgaggatgt
    11461 acgcccgccg ttgaccaaca ggacgaatac tgacgcgcca gcttttgcgc ctgctggctt
    11521 tcacgcaggt tagtgccggt gatgagtagc ccattaactc ccgcgtcaaa agcgcgcgct
    11581 acaacatcat cacggtcttt cgcaaattgc gaactggtca aattaacgcc gatatcaaac
    11641 atcctgtact ccatatgaca accgccctga cgggcggttg aatttattct tcagtttttt
    11701 cgctttctgc ttcagcgtcg ttttcctctt cccgatttcg ccctttacca acgtaaaagc
    11761 gtgagaagaa gacaccgatt tcaaacagac agtacatcgg gatcgccaat agcgtttgcg
    11821 agaagacatc cggcggcgtc agcaacatcc cgacaacgaa tgcaccaacc agcacatacg
    11881 ggcgtttttt gcgtaagtct tctggcgagg taatccccat ccagcacagc agcacaattg
    11941 ccaccggcac ttcaaaggag acaccaaacg ccataaacag cgccataacg aagcttaaat
    12001 agctggcgat gtcggtggat acctgcaccc cttccggcgc ggtattggca aggaagccaa
    12061 atgccagcgg aaagaccaca aagtaggcga atgccatgcc gatataaaac agcagagagc
    12121 tggaaaccag cagcggcacc accaggcgac gttcatgctt atacagcgct ggggcgataa
    12181 atgcccacac ctgatagaga atcaccggcg ctgacagaat cagcgacacc ataaaggtca
    12241 gcttgatcgg cgtaaagaac ggcgaggcca cgtcggtggc gatcatcgtt gaaccttgcg
    12301 gcaactgctt gatcaatggc gcggatacca ggtgatagat gtcattggcg aaatagacca
    12361 gacacaggaa tatcacgatc accgcgataa tgcagttcag cagacgctta cgcagctcaa
    12421 ttagatgcgt gataagcggt tgagtatctt ctacagacat gtttacggtt tatcactcga
    12481 cgaaggggaa ggtgcagcgg ttttcggttc agcgtccgca gcaggtttta ccaccggctc
    12541 tggcgtggtt tctggcttct gttccggcga actggcctgc gtttgtgcag cggcaggcgt
    12601 tacgccctca tgcgcagctt cattatcttt caccaccggg ttatggatgg tgtgcgcttc
    12661 atcgctcgcc ttttcaggat cgtttgcaac gtaggaacgc ttcatcgact ccgcggcctg
    12721 gcgtagttca tccatcgacg ctttcagttc gggcgtcagg ttagtgaggc tcgccttttc
    12781 aacctttttc agactgtcct gaaactcctg gagttttaac tcctgggtca gttcgttctg
    12841 caccgttgtc gccagtgaac gcaacgcgcg aatccagccc gctaccgttt ttaccgccac
    12901 aggcagtcgt tgcggcccca gaacgacgag gccgatgatg aacaccaata gcagttcgct
    12961 aaaaccgata tcaaacacgg attacacctg ctctttatcg tggcgcttcg cgtcttctgt
    13021 tttagcctgt tcctgattcg tatccgcctg cttatcggcg atagttttcg cagtaaaatc
    13081 agcatcctga ctggttttat cctgctttgg ttcatcatcg ctcattgctt ttttaaagcc
    13141 tttgatcgac gcaccaagat cggaaccgat ggagccgagc tttttggtgc caaaaagcag
    13201 tacaacgatg acggcaataa tcaataactg ccaaatactg ataccaccca tacatgttcc
    13261 tctgtggtag atgatgatta aacaaggccg cattatacgt tacacgaccc gccttgagcg
    13321 atgaaaaaat cagcgtgttt tgcgccaacc gacaaaccag gcgatcagac cacctgccat
    13381 taaccagccg ggcatcagcc cccattcagg tcggctgacc aacaagaatg tgccacttaa
    13441 tactaacgta gcgccaattc cgagaaaata acgcgattgt ccctgacgta catgatttga
    13501 ctgaagctcg cgggcaatct tatcaacact gtgctgtaaa tacttgccct ggcgcaaact
    13561 gtcgtaaacc aattcaggca gttctggcat tttttcgacc cagaacggcg ctttttcttt
    13621 aaatgctctc accagggcag gaataccgac ctgatcttta atccacgact ccaggaaagg
    13681 cttcgctgtt ttccataaat cgagctgcgg ataaagctgg cgtcctaccc cttcgacata
    13741 gagcagggtt ttctggagta acaccagttg cggctgcact tccatattga agcgacgcgc
    13801 cgtattaaac agatttaaca gtacatgtcc aaacgaaatt tcggccagcg gtttctcaaa
    13861 gataggttca cagaccgtac gaatggcaaa ttcgaactct tcaacgttgg tatctggtgg
    13921 cacccagcca gaatcgacgt gtagctctgc cactttgcga tagtcgcgat taaagaaggc
    13981 gataaagttt tctgccagat agcgtttatc ttctttgttt agcgagccaa caatcccgca
    14041 atcaatgccg atatatttcg ggttttccgg gtgttcatag cttacgaaaa tgttgccagg
    14101 gtgcatatcg gcatggaaaa agctgtcgcg aaagacctga gtgaagaaca cctgcacgcc
    14161 acgttccgcc agcaatttca tgttggtgcc atttttctcc agcgtcgcaa catcagacac
    14221 cggaatgccg taaatgcgct ccatcaccat catcccttca ctacaatagt cagggtaaac
    14281 ttccgggatg tagagcatcg ggctgtcttc aaaatttcgc cgaagctgaa tggcgttggc
    14341 agattcccgc agcaaattca gttcatcaat caaggtcttt tcgtactcgc gcaccacttc
    14401 ggttgggcgc agacggcgac catccggcag caaacgcggc acccagcgag ccagacggta
    14461 gataagtttc agatccgctt taataaccgg caaaatatcc gggcggatga ctttaatcac
    14521 cacctcttta ccattcgatt tcaatcgcgc ggtatgaacc tgggcgatag aagcagaagc
    14581 cagtggcttg atttcaaaat cgtcaaacca cgcttctacc ggcaagccgc ccattgcagc
    14641 ttcaatctgc tgcttcgcca gcttgccatc aaacggagca actttgtcct gcaataacgc
    14701 cagctgatcg gcaatatgcg gtggaaaaag atcgcggcgg gttgataaca tttgcccgaa
    14761 cttgatccaa accggcccca gttcttgcag ggccagtcgt agtcgctcac ctaaaagttt
    14821 gtctttatgc cgatttggca tccagaataa tgagtatcgc catagccgta gcggcagggt
    14881 gatacgcatt ttggggatca gttcatcaag tccgtagctt aaaaaagtgc gaatgatgaa
    14941 atataggcgc cgtacttcac ctggcgtcat ttagcctcca gtttttccag ccgtttggtc
    15001 agggcatcaa cagcacgctc gacggcagcc gtctcttccg caaaccaggc cacttcaagc
    15061 ggaccgggtg ccatacgcca ctcttcagta atggcttccg ccacataacg ttgctggcgc
    15121 ttaatgccgt gatgcaggaa ctttgcgcct ccgcgcatgg ctttgctgat tccttcagcg
    15181 gcgatatcac cggtataagg ggccagcagt tccgcagggt cgaactctgc cagatctgcc
    15241 agcgcaacga agttttgcac cacctgaata tcgccctgca cttccagctc accactgcga
    15301 atcagtgcgg taagctgctg gcgatcgcga agtttcggca acacactggc gtaggcgata
    15361 acggtgcagt cagcatcgcc tgcccattcg cccagtacat caacctggcg ttcgctgaac
    15421 accagaatca atgacgtcga aaagcctttt acctccacgc gcaatacttt acccagcaga
    15481 cgcgagcggg ctgttttcag cgcgggtgag cgatacagga aggtgttgag cagactttca
    15541 attcctgccg tcactaaagg tttaaaaggc atttccggtc tcctgtcaga acttataacc
    15601 acgatgcagc gccacaaccc ctgccgtcag attgtagtag tcgacacttt cgaatccggc
    15661 atcctgcatc atggctttca gggtatcctg atcgggatgc atacggatgg attctgccag
    15721 ataacggtag ctgtcggcgt cgttcgcgac cagtgagcca atacgcggca gcacatggaa
    15781 ggagtatgca tcataggctt tgctcagcgg ctcgataatt ggcttcgaga actcaagcac
    15841 cagcaggcgg ccgccgggtt tcagcacgcg atacattgaa cgcagtgctt tatctttgtc
    15901 ggtgacgtta cgcagaccaa acgaaatggt gatgcaatca aaggtgttat ccgggaacgg
    15961 cagcgcctca gcgttcgcct gaacatactc aacgttgcca atcacaccga tattacgcag
    16021 cttctcgcgt cccattttga gcatggattc attgatatca gcaaggacca ctttgccagt
    16081 ttctccgacc aggcgggaga atttcgctgt caggtcgccg gtgccaccag ccagatccag
    16141 cacggtctgc ccacggcgta cgccgctgca atcaatcgtg aatcgcttcc acaaacgatg
    16201 aataccaaat gacatcaaat cattcatgac atcgtatttt gatgccacgg aatggaaaac
    16261 gtgggcgacc atatccgctt tttgttcctt cgcgacggtc tgaaaaccaa agtgcgtcgt
    16321 ttcttgtgac ttatccacca tctcaatgcc tgctcatcaa aaaattgttc cagaagtgta
    16381 acagattggg cgtcgatgcc ctagatttct acccggctta actaccccta atgggctagc
    16441 gcgactgctg attatattca tcatcgcgtt gataagcttc atcattcggc tgctccggaa
    16501 ccgaccgaag tcgatactct tcattctggc tcaccgcctg ttcagccaaa tccggattaa
    16561 tctcgcgttt aatttctact cctaaaccgc gaaacgcttc tgcctgcgcc agcacatttc
    16621 cgcgacctga agagagtttt ttcattgcct ggcggtagtt atcctgcgct ttgtcgagac
    16681 tttgaccaat cgcggacatg tcatcgatga acaaacgcat cttgtcgtac agcttgctgg
    16741 cacgatcggc gatttgctgg gcgttgcggc tttgatgctc ataacgccac aggttggcaa
    16801 tagtacgcaa cgccaccagc agcgtagtcg ggctaaccag catgatattg tttttcaacg
    16861 cttcggtgat cagctccggc tggcggtcaa gcgccagtaa aaaagcgggt tcaacgggaa
    16921 taaacatcag cacgtaatcc agagttcgca gccccggcag ctgttgataa tctttgcgtc
    16981 ccagcaaacg gatatggtta cgcaccgacg cgatatgttc ctgtagcgcg ctttcgcggg
    17041 tgtagtcgtc ttcggcgtta aaatagcgtt cataggcgac cagcgtcatt ttggcgtcga
    17101 tcaccacatc ttttccctgc ggcagccgca cgatgacatc cggctgcatc cgcgagcggg
    17161 cgtcattttc gatgctgacc tgggtttcat attcataccc ttcacgcaga ccggaagcct
    17221 ccagcacccg cgtcaatact acctcgcccc agttgccctg ggttttattg tcgcctttca
    17281 gcgcgcgcgt caggttgatc gcttcctggg ccatttgcgc gttgagttgc tggagattgc
    17341 gaatttcgtg ggtcagggta tggcgttctt gtgcttcttt accgaagctg tcctgaacct
    17401 gacggcggaa accgtccagt tgttcacgta gcggcgacaa caggctgttc agactctgac
    17461 ggttttgctc atcaacccgg cgattgctgt gctcaaaaat acggttggcg aggttttcaa
    17521 actgctcact gaggcgctgc tcgctgttaa tcatctggcg aattttatcg tcagcatgtt
    17581 gctgtgcggc ttccatccgc gtggttactt cacgcagatc ggcctccaga gaggtgttaa
    17641 tactttgcag gctgcgcact tcgttattga gtaactcgca ctctgcacgc cagtgctcgc
    17701 tttgggtaat ttgttgtttt gccgcgctta actccgcgac catctcttca cgttcagcta
    17761 attgctcggc tttttgctgc gcatgttgat aactggcaaa cagccagcca attgccacac
    17821 ccaccaacgc aataactgcg taaaccatga ttgagaaatc cacaacgcct cctgacgcaa
    17881 caacttaccc gcacaaaata gaattgtgct gtacaaacgt ccagttggaa aggcatttcc
    17941 tgcgaggcgc tacgcaaaaa tacaaaaggc cgaacgcgtc ggccttcaga caggagaaga
    18001 gaattacagc agacgacgcg ccgcttccac cacgattttc accgcttggc tttcagtttg
    18061 tttcatcgtc tcagcattcg ggatctcttg ctgggtgcgg ttaacgataa cacccgctac
    18121 cataccggca cgcaggccct gacttgcaca catggtcagc agggttgcag attccatttc
    18181 atagttcatt atgcccatcg cctgccactc ttccatagaa cctttaaagt gacgaactac
    18241 gcgaccagag taagtatcgt aacgttcctg acctgggtag aaggtatcag aagaagctgt
    18301 cacgccaacg tgagttgtcg cgccaatgga tttcgcagct tcaaccagcg cagtcgtaca
    18361 ttcgaaatca gcgacagccg ggaattccag cggtgcgaag tgcaggctcg cgccatccag
    18421 acggacagac gccgtggtaa ccaggacatc acccacatta atatgcggct gaatagcgcc
    18481 cgttgtaccg atacgcagga aggtgcgaat gcccagctgt gccagctctt caacagcaat
    18541 agaggtagac gggccgccga taccggtaga gcagacgata acaggtttac catccagctc
    18601 tgcacgccag gtagtgaatt cgcggtgaga tgccagctta accggcttat ccatcagcgc
    18661 ggcgatcttt tccacacgat ccgggtcgcc agggacgatg gcaagcgtag ccccttgtaa
    18721 atcgttttta gtgaggccga gatgaaaaac atcagacttg gacatataca actcctctgt
    18781 gaatcggttt agtcagaaga agcaaaaaga cactttaccg aagggtttaa cattttttcg
    18841 tgatactcat caccatgacg caaatgcgtt gcataaattt acccaccaaa agtgattcaa
    18901 atcacataaa tcactcttca ttctgcagga ctgcataaaa gatgagccga ttcaggcaat
    18961 gtgatttgtt gcatgctgac tatagttaac attgcgcaac atttttataa gggtacggag
    19021 aataccatgg caacaacaca acaatctgga tttgcacctg ctgcatcgcc tctcgcttcg
    19081 accatcgttc agaccctgga cgacgcgatt gtggcgggct tcacctctat cccttcacaa
    19141 ggggataaca tgcctgctta ccatgccaga ccaaagcaaa gcgatggccc actgccagtg
    19201 gtcattgtag tgcaggaaat ttttggcgtg catgaacata tccgcgacat ttgtcgccgt
    19261 ctggcgctgg aggggtatct ggctatcgca cctgaacttt acttccgcga aggcgatccg
    19321 aatgattttg ccgatatccc cacgttgctt agcggtctgg tagcaaaagt gcctgactcg
    19381 caggtgctgg ccgatctcga tcatgccgcc agttgggctt cccgcaacgg cggcgatgtt
    19441 catcgtttaa tgatcaccgg attctgctgg ggtggacgta tcacctggct gtatgccgcg
    19501 cataatccac agctaaaagc tgcagtggcg tggtacggca aactgacggg cgacaagtcg
    19561 ctgaattcac cgaaacaacc tgttgatatc gcaaccgatc ttaacgcgcc gattctcggc
    19621 ttatatggcg gtcaggataa cagcattccg caggagagcg ttgaaaccat gcgccaggcg
    19681 ctgcgggctg ctaatgcgaa agcagagatt atcgtgtacc cggatgccgg gcatgcattc
    19741 aacgctgatt atcgcccgag ctatcatgcc gaatctgcga aagatggctg gcagcgtatg
    19801 ctggaatggt ttaagcagta tggagagaag cggagttagt ttcctcttag tctatatttg
    19861 agatggtgag taaacgagtg cgttttgctg ccatctctgt cccccaaaaa ataatcagta
    19921 tgaattgatt ggagattacg actccgctat ttgaccttaa gaaagtcggc aagtcatagt
    19981 cataaatgag tctcttcaat ggaagacacc ttagagattt accatcaact cccattcaca
    20041 aacaaacatt aattattatc aacacattca cattaattat aaatcatctc agaccttcct
    20101 gataaaaaga tattaaagtg aaacattttg tttttcacat tttagttcat cacttttagg
    20161 cgtaaataaa ggtaaaaaaa acaacaatac tatttctctt tcacaaaaga attaacaaaa
    20221 ggtttttcaa ggatgagtga tattatttac ctgaccataa ccggagaaca acaaggatgt
    20281 atttcctctc gttgtggaac gagcgcatca ataggtaatc gctggcagat tggccatgat
    20341 gatgaaatct tcgcgttctc gctctctaat agtattacga atacaggcaa agggtcataa
    20401 ttgcatggcc ttagtttctg taaattgatt gacaaaagct ccccgttact tattaacgct
    20461 attaacaata acgagcagtt gttcatggag tttgacttct atcggatcaa taggtttggt
    20521 cgatgggaga aatattacaa cattcaactc cgcggcgcgc tcttatcagc aatcaatcac
    20581 ctgtttactg agaacaatct ggatacagaa gccatcactg tcagttatta atatatactc
    20641 tccaggcatc tgattgctaa tactgaattt agttattggg catttcccga taactacaac
    20701 cgtctgttta taccacggcc aaaaactaaa gctggtaatg gtttcaaaac acttaatagt
    20761 aaagcggttg gcagactgct tgctgctgtc ggcatataca atgggaatat cgaaggattc
    20821 agggaaacag cagacaagtt ggggggagat gcacctgctg gttatgatca atttatggat
    20881 aacaaagggt tactcatcgc tggtgcttca atagttgctg gagcagttct ggggagaatg
    20941 agactccctg atttggaatc acttgagcat ttcggagcca gaggagcagt atcaggaaga
    21001 ccatttaatc ccgagcttgc cggtggtcca atagaaaacc tgacgacaga tggcgtaaca
    21061 atcaacaggg aaggtatcgc tattgttgaa aaacatatag ccagatttgg tcatgatcct
    21121 gtaaatgaag tgatgatcaa tagactgaaa gacattgaga aaggaaaaat ccccccagaa
    21181 caagtagatc ttaacttcta tactcatgag tgtcgggaat atcagcgtta ctgtaatctt
    21241 ggttgggaaa caggacaacc tgatggtgac gcaggctatg cactctggaa tcatactgga
    21301 tttgccccta tatttccaga catctgttat cacttaaccc attacaagcc cgctgcc
//
LOCUS       DAJTVK010000069        21193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000069 DAJTVK010000000
VERSION     DAJTVK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.069"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..211)
                     /locus_tag="OO372_002572"
     CDS             complement(<1..211)
                     /locus_tag="OO372_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6342035.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIA"
     gene            complement(208..1769)
                     /gene="casA"
                     /locus_tag="OO372_002573"
                     /pseudo
     CDS             complement(208..1769)
                     /gene="casA"
                     /locus_tag="OO372_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(1867..4566)
                     /locus_tag="OO372_002574"
     CDS             complement(1867..4566)
                     /locus_tag="OO372_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCR6342036.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGI
                     NTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLV
                     GSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGL
                     QEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKE
                     DSSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            complement(4761..4913)
                     /locus_tag="OO372_002575"
     CDS             complement(4761..4913)
                     /locus_tag="OO372_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6342037.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(5178..5912)
                     /gene="cysH"
                     /locus_tag="OO372_002576"
     CDS             complement(5178..5912)
                     /gene="cysH"
                     /locus_tag="OO372_002576"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6342038.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(5987..7699)
                     /gene="cysI"
                     /locus_tag="OO372_002577"
     CDS             complement(5987..7699)
                     /gene="cysI"
                     /locus_tag="OO372_002577"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6342039.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(7699..9498)
                     /gene="cysJ"
                     /locus_tag="OO372_002578"
     CDS             complement(7699..9498)
                     /gene="cysJ"
                     /locus_tag="OO372_002578"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6342040.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            9817..10179
                     /gene="queD"
                     /locus_tag="OO372_002579"
     CDS             9817..10179
                     /gene="queD"
                     /locus_tag="OO372_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6342041.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            10257..11528
                     /locus_tag="OO372_002580"
     CDS             10257..11528
                     /locus_tag="OO372_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6342042.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            11519..11779
                     /locus_tag="OO372_002581"
     CDS             11519..11779
                     /locus_tag="OO372_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6342043.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            11796..12371
                     /locus_tag="OO372_002582"
     CDS             11796..12371
                     /locus_tag="OO372_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR6342044.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(12519..13379)
                     /locus_tag="OO372_002583"
     CDS             complement(12519..13379)
                     /locus_tag="OO372_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6342045.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(13376..14155)
                     /locus_tag="OO372_002584"
     CDS             complement(13376..14155)
                     /locus_tag="OO372_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6342046.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(14133..15542)
                     /locus_tag="OO372_002585"
     CDS             complement(14133..15542)
                     /locus_tag="OO372_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6342047.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(15564..17018)
                     /locus_tag="OO372_002586"
     CDS             complement(15564..17018)
                     /locus_tag="OO372_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6342048.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(17088..17904)
                     /locus_tag="OO372_002587"
                     /pseudo
     CDS             complement(17088..17904)
                     /locus_tag="OO372_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            18222..19499
                     /locus_tag="OO372_002588"
     CDS             18222..19499
                     /locus_tag="OO372_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6342049.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            19526..21011
                     /locus_tag="OO372_002589"
                     /pseudo
     CDS             19526..21011
                     /locus_tag="OO372_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         5098 a         5614 c         5220 g         5261 t
ORIGIN      
        1 cggctatcag cgccagtgcg gtgatacgcc actcaggttc atcctgccac tttatttgtc
       61 tgttcttcag aaacaggcga aatccatccg tcagacaaac atcattaacc gtcgtactac
      121 gccgcagact ggcacgttcg ccgcgtttct cctgcaattc ctcgtgccat ttgcgcagcg
      181 tggctttgtg ctcctctttt acaatactca ttcagcagcc tcctgctttt tctccctggc
      241 ggctttagca ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg cggtcatgat
      301 gcgttccaaa tcaacgggct cataaggatt ggtgaataca cgctcgtcaa aatcctgacg
      361 cgcgaataac caaatttcct tattccattt gctgagtaat tcatccgcat cctgaccttc
      421 ttcaatttgg cgcactaacc tcaggaagcg atgctgagtt ttgttccaga agtcgatatc
      481 cacaaaacta aaatcacccc ttgcaccttt tggatcggag aaccatgctt ctttcaatgc
      541 actccgtaac agacttagaa tccgtgaagc cgtttgcgca gccagccgca gtttcggtat
      601 ctggccttct ttttattgag cagcagcggg aaatgatgtt cgtaccaaca gcgcgctttc
      661 atgttgtcga aatcatagcc aaatccccac aggcccactt ttgcctgctt gagactgctg
      721 gcattaaaga gtttcaccac cagcgcggga agttccgtat tgttttctga cttacccgtt
      781 tcgataaggc ctaaccagtc gcgccagatt aaaccgcccg gttgtggttt gaccgagtaa
      841 aactcgccgc cctctttaag tagtacacgg taaggcgtta agggatgctg ccacatggca
      901 tagttagcgc cgtaattttt ggtcgtcatc aggctcagaa gcgcgtcgct ctgctcaccg
      961 caaatatcgc agttgccgac tgtggtggta ttaaaatcaa tacgaatacg gcgaggcatt
     1021 ccccagtacg cctggagttt attgacctgc tcatcggtta ccaccgcacc ggccagttcg
     1081 ctgatgcgcg tcgggccaag ccaggggaaa accagatcgt caaatttttt gggtagcggt
     1141 aaatcggctt catcctgcgg catgacattg atccacagtt tgcgccacaa gggagtttgt
     1201 tgattaccct gatattcctg taattcaatc agagtggtca tcggcccacc gccgcgtaaa
     1261 ccggtgcggt agcctttgcc acctgacggc gcattcaact gtagggagaa cagagctaac
     1321 gcagagcaat gtgggcacag gtattccgtt acaccacgct tgataaagtg gtctttatta
     1381 aacttcgttg tttgagcgcc gggaatctca ggcagtagcg aagcgaccgg aactttatct
     1441 ccggtgagcg cctcgaaatc ctgtataaat gaaggggaat ctggcccaaa ctggaaagca
     1501 tgttctaatg acagcaatgc ttcccgtagc ttttcagctt ccagtccgtc ttcccagata
     1561 tcatcccaac gacgatgatc ttttggcgcg aagctggttt gtagtagccc cagcaaaaac
     1621 tgccataccg caccctggag atctgcccgc ggcgcagaaa tatcgacaac attttcatcc
     1681 gccagatcga ctggcgccag tttgcctgtc gttccgtctt taaaacgaac gggcaaccac
     1741 ggggttgtca gaagtgaaaa cgagttcata cgcatcctct cgtaaaaaag accatcccag
     1801 gtcggttgtc catctttcca tccttttcgg tgtatcaact attcagagag tcctgtcgca
     1861 gacatctcaa ccaaccttgc caatcaatcc ttccgtagcc gaatacccac aactttcgtc
     1921 atcgttgact aaaataacgt ttgccatctc cggatcttgt cgctgttcaa tgcaccacag
     1981 cctgaacgcc tctccctcca gtaaagaaaa ctcatcccga tgttttttcc accagcttcg
     2041 gcgcactctg acaacgctca tttcccatgc gtgagcaccg gtggcataag gcttcaccac
     2101 accgtcaata caggtagcca gccacaggga aacagactcc tcagccagac gtgtcgacag
     2161 tttttctggg aggtaatcgt tgatattggc ggcatagccg ggcctgaagt tcagcacaaa
     2221 ctttttagcc attgcgcgat cacagtaata tttgcccact tgctcctgct cgctgcgggc
     2281 aaatccttcc ggcattacca cgtcctcacc gtagacggat tcaataagaa gccgggctgc
     2341 gtgtggcatc tgaatagcac cttgctcacg cagcacacgc tgcgtcagcc agattcgccc
     2401 atgatcggga tagacaaatg cactgttacg catggcactg ccaaaccatt cgtcgccagg
     2461 agagtcgtcc cagacgggag ccagaatcag caattcaggg ggggaacgct cgtcctttcc
     2521 gtcacgcttt aactgaccat taatatcgcg gatatgccgc tgtaatcgtc ctgctcgctg
     2581 aatcagcaaa tcaacagggg ccaggtcgga gatcatttcg tccaggtcac aatcgacact
     2641 ctgctctaat acctgagtac aaataaggac ttttccggca cgctgtgagc tatcttcttt
     2701 accaaagcgt gccagtgtct ccgtttcaat tcgctggcga tcgctaaaag caaagcggct
     2761 atgaaaaagt gaaaggctgg aagcaggaat gacgccgcgg gcaagcagct gacgatgaac
     2821 cttaatagcg tcatcgacag aattccggat ccaggcgatg catttcccct gacttaccgc
     2881 cgattcgata cgcgcaatac actcttgttc actatgaagc caacccacgc tgacgctacg
     2941 ctcaacgtct ttgcgcgtcg ctacccggtg tgagttcaca tcggatttcg tgacatgcgt
     3001 cagccagggg taatcatcct tttccaggaa cggagcttct tgcaggccct ctgtaccccg
     3061 cgcaaaggca gcgacgagtt tgtcgcgctg ctgttgagat aacgtggcag aaagcaaaat
     3121 aacgctgttt ccgccacgcg cctgccgctc gatcaaccct tcaagaatgc acgacatgta
     3181 agcgtcacag gcgtggatct catcagccag caggattttg ttactcagcc ccagaagccg
     3241 cagattatta tgtttgaacg gcatcaccgc catcatcgcc tgatccagcg tgccgacgcc
     3301 aatttcagcc agcaacgcct tcttgttact gtcggcaaac caggccgcac atccctgact
     3361 gaacgtttgt tcatccggtt cttctgaccc gactaaatca ccagaccaga gtgattcatt
     3421 aaagcggtcc attaatgtgc gggcactatg tgccagcacc aggctggggc gggattctgg
     3481 cgaatagaaa gcaagccagg ttttgaccag ccgatcgtac atggcattgg ccgttgccat
     3541 tgttggcaga ccaaaaaaca gcccctgagc cttccccgca gctatcaacc tgtgcgccag
     3601 gataagcgcc gcttctgttt tacctgcgcc agtcacatct tccagaataa ataactgcgg
     3661 tccttgctgg ctgatatcca gatccagcac cttttgctgt aacggtgtcg gattctcaat
     3721 aaaaggaaac agcgtattaa ttccgttgaa aggcgcgact tttgctttta gaggaaagac
     3781 ggttaaggcg ttttgagcct gaactaaagt tttctgccag taacctttaa tatccattgg
     3841 gtgtgcgacg cgtggaaaaa atcgcgttga cgaacccgtc cagtctgcga gcacgacggt
     3901 tgcagagata taccaggaaa ggtgttttat aagttcaatg ccctcatcat catcccagaa
     3961 tgcggggatc tctatgagcg gaaacagtgg tttgatttca aggagaaaat ctcgtgctgc
     4021 agctttgtct tcaggcagaa aattatccaa ttcatccata cgatcagggg gccaaccatg
     4081 atgccctgtc gtcacgggca tccacatttc tatcactcgc ctaagtttgc gcggagagag
     4141 cgaagatgaa ggaagtaact cctgacattc acttaaataa tgattccaca accagtagcc
     4201 cagcgttgaa tgagagattt tttcgtaatt ttttctggca ccttcctgaa tcttgagttc
     4261 agggggcagg taaagttgtt gaaaagagcg ggcaaatttt ccaatatcgt gccagcacag
     4321 caaccaggcg aaaaattgag ccgcctgttc cttgtcagaa atccctaatt gacgaaaata
     4381 atcagccagc ccgaagcaat ttcttttaac cattaaatag cccatcgcgg ctacatccag
     4441 cgaatgccag caaagaaggt gatagtcgtc gcccccgtct ttctcgccgc gtcgggtttt
     4501 tccccagaaa tcaaaaaaag tcacaatatt tttatccttc agtaaactta aaggatattt
     4561 acgcatatat taaaaattat ctgtgatata tatcattcaa taaaatattt acattatgaa
     4621 taatcaaata attttcatcc gtaatatata tgaataataa acataggcat aatattcaca
     4681 taaataatat aaattaaaaa agggtgcagg agaccaaacc atcacaattc agtacgcggg
     4741 gttttacccc cgcgtggtgg ctacttcttc gattcgtaag cgagaactgc cttaaactcc
     4801 ataccacgtt ctctgatact caactcacac aacgagtcgc ctaccatcag cgtgaatccc
     4861 agtactgtaa aacacagcac tatgattgcc acaagggcat attttgtcag catgtcttgc
     4921 ctccctgcaa agaagagact aacattcgaa ttgtttgggt tcgaacgctg gcctcaggtt
     4981 gatagaaata tcgcctgggg cttttgtcca tctggaacct cgcgaatgct taacgccaga
     5041 cagcctcaag cacccgacgc cattctatac ctgataattc ttccagcggc gttcacgttt
     5101 aaaatcagga atactgctcg caaaataaaa acgcccccat catttctgac agaggcgttt
     5161 aatttatccg gcaatattta cccttcgtgc aatccacatt ccctttttaa gccaaagaaa
     5221 cgtgtttctt cttccgacat gccaggttcc catttacggg ttgtatgggt atcaccgacc
     5281 gataaatatc cttcatccca taatgggtga tatttcaggc catgtttttg caggtactga
     5341 taaatagttc ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt
     5401 gccagcaccg gtaaattggc gcgactgccg gattgttcac ggcgcaggcc agcaaaccag
     5461 gtttgcgcgt taagttcttt cagagcccgg ttcatcggtt cgactttgtt gatgtcattg
     5521 tacttttcaa tgccttcaac gccctgctcc cacagttttc cgtagcgtgc ttcctgccag
     5581 gctgcgcttt cggtagcacg gtacactttc aggttgagct tgagtttgtc cgttaactcg
     5641 tcaataaagc ggtaggtttc cgggaacaag taacccgtat cggtgaggat caccggaata
     5701 tccgggcgaa tttgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa
     5761 gaaagtacat attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc
     5821 agtttttcca gttcggcgtt agtttccgcc agcgccagaa tacgatctac cttcggcagt
     5881 tcgttcaggg cgtttagatc gagtttggac atagattcct cactgtttgc cttgcctgat
     5941 gcgacgctca cgcgttctta tcaggcctac aagaccgggc tgatggttaa tcccacaaat
     6001 cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag tcgccgaagc
     6061 cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc gacgccagga
     6121 tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca atgcggttgc
     6181 cgccaagatg caggttgtag cgacccggcg ctttacccac caggcccact tccgccagca
     6241 tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc tcatcgctga
     6301 caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg aaacgctctg
     6361 cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag ttttcacgct
     6421 gcggcgtgac ggcattcatt aacccgctct ctttggcgat cttctcgatc ttcgctttct
     6481 cgctttccgg tacaccggtg atgatcagat tctggttcgc cgtaatgcgg aaatcgcctt
     6541 tgtggatctt cgcgatctcc agcaggccgg ttttcagcgg acgccccgga taatcaagga
     6601 tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc ttaacccagc
     6661 caatacgatc gcctcgtccg gtgaactcat atggacggat cggttcaaat ttgatccccg
     6721 cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagc gtgtatttgg
     6781 ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc acgacggctt
     6841 cggccaccgc cagcgtatgc tccagcggca gatagccaaa ctcgctcgcc gtgcgggcgt
     6901 aggttttctt gttgccgtgt tcgatagaaa gcccaccgcc caccagcagg ttgaagccta
     6961 ccagcttgcc gttttcggcg attgccacga agttcatgtc gttggcatgc agatcgatat
     7021 cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag gtctgaccaa
     7081 ggatcggttc ttcatcagta gtggcgactt tttcctggtc gagccagatc tccgcatacg
     7141 cgcgggtacg aggcaacaga tgctcagaaa tcttcttcgc ccactcgtac gcttccgcgt
     7201 gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg tcgttagctg
     7261 tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg acgttctttt
     7321 tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg ccatagatgg
     7381 tgttttcacc ggcaaatttg tcgatcgcct gccactgttt agtggtaatc accccacccg
     7441 gcagacgaca gcgaagcagc atcgcgtggc gcggctccag cttctgttca gcacgttcgg
     7501 cacggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc aggaagttgt
     7561 cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg ccgcgcaggt
     7621 agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc actaaaggcc
     7681 ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta cgcgcagctc
     7741 acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa tcacttccag
     7801 aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat aaatgtgggc
     7861 accgtcattg atccagcgcc acagctccgc gccctgttcg cgcagtttgt cttgtacata
     7921 aactttttct ttttggtcgc gcgaccaggc aagatcgata cgcgtcagca cgccctcttt
     7981 gacgtagcgc tgccactcca cctggtagag gaaatcttcc gtaaagtgcg ggttgccaaa
     8041 gaacagccag tttttacccg gcgcttcgtc ggcggcgcgt tgctgcataa aggcacggaa
     8101 cggcgcgatg ccggtaccgg ggccaatcat aatcactggc gtttgcgggt tagcgggcag
     8161 gcggaaattg tcgttatgtt cgataaatac gcggacttcg ccctcttctt ccacgcgatc
     8221 cgcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt aacgcaccac
     8281 accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcga tggaatacag
     8341 gcgcggggtc agcgggcgca gcaggttaat cagcgcttcg gcatccagtt gcgccggaga
     8401 gaaacgcacc atgtcgacaa tcggcgtggt cgcggcgtaa tgctgtaact tcgctttatc
     8461 gcccaccagc ggcagcagcg tttcgctgcg ggtaagcgtg gcatagttct caacaatgtt
     8521 ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag gcaacgtttt
     8581 gccctcgacg gtgacaggtt catcgccttt cagccacagc agttcgacaa gttctttcac
     8641 cagtgccggg tcgttctgat accagacacc cagcgcgtca cccggctggt aacgcaggcc
     8701 tgaatcgcct aagtcaattt cgatatgacg aacgtctttt tcagagttac gcccggtaat
     8761 tttctggtta accgaaaggc tcgccaccag cggcgcgtct ttgctgtacg ggctggtgtg
     8821 gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag gcgcacgcga
     8881 tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt attcaacatc
     8941 ggcatcgaca cggtcgagca ggcgttcacc acccagttcc gccagcttgc tgtcgaaatc
     9001 tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca cggcaaacgc
     9061 ggtgttttcc agctttggcg cttttttgga gaacaggaac ttatgcagcg cgacggcttc
     9121 ttccggcggt tccccttccc cttgcgttga cgtcactacg atgagcagtt tttcgctggc
     9181 gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt ttgctgctaa
     9241 taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg aggcggagat
     9301 aatagttata cccggcattt ctgcggctgg cgctggcgtc gctgcaagcg cagcaggctg
     9361 ctgattgagt acgccccaga aatagccaga aacccaggca agctgggtag gcgttaaatc
     9421 ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca acgcggaagg
     9481 tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt ctgcgccctg
     9541 tcagcgccca taaaacagaa gagaagataa ggttaacggg gtgaacggtg tggattaaag
     9601 acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata gtctttaaca
     9661 acaaaataga ttaaccaacc caatgaaaaa caaatgaatt tagccaatca ttaagataaa
     9721 tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc tttccggtac
     9781 tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt atttaaagat
     9841 ttcaccttcg aagccgctca ccgcttacca cacgtcccgg aagggcataa atgtggtcgc
     9901 ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga tccgcatacg
     9961 ggctggatta tcgatttcgc tgaactgaaa gcggcgttta agccaactta tgagcgcctc
    10021 gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga agttttagca
    10081 aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt gatggtaaaa
    10141 gaaacctgca ccgcagggtg tatctatcgc ggcgaatgat aagagtgtgt cggcggtcaa
    10201 tttcccttaa gtaacgctat gttagggtgt tgtgttctgg atatctgggg catgacatgg
    10261 aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct tgcgcgttac
    10321 gctgcgcgcg agcgggttta tccgttttgt tactggaacg cgctgaaatc cccggcagca
    10381 aaaatctttc cggcgggcgg ttatataccc atgcactcgc ggaactcctc ccgcaatttc
    10441 atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttccctg ttaacgcccg
    10501 atggcgtaac gacgttttcc agcttacagc ccggcggtga atcctggagt gtattacgtg
    10561 cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa tgcatccccg
    10621 gagcgacggt ggatgcactg tatgaagaaa acggcagagt ctgtggcgtt atttgtggtg
    10681 acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc gtcctggctg
    10741 aacgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcgttgggg atcaaagaag
    10801 tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat aacgaaggcg
    10861 cagcgttgct gttcagcggc ggaatctgtg atgacttacc cggcggcgca tttctttata
    10921 ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt acgcaaagtc
    10981 gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg cgcccgctta
    11041 tcaaaaacac ggaatcactg gagtatggtg cgcatctgga gccagaaggt ggcttgcaca
    11101 gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg ttgcgcagtt
    11161 gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc gcacaggcgg
    11221 cggcacaaac gctaataagc gcctgccagc accgcgagcc gcaaaatctg tttgcgcttt
    11281 atcatcacaa cgtagagcgc agcctgctat gggatgttct acaacgttat cagcatgttc
    11341 cggcgctttt gcaacgccct ggctggtatc gggcgtggcc tgcgttaatg caggatattt
    11401 cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag ttattctggc
    11461 gtcatttacg tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg agtctgcgat
    11521 gtctgtagcc cataatctct ggcgcgttgc tgatgcgccg cacattgttc cggctgactc
    11581 cgttgagcgt cagatggtac aacggttgat taacgcctgt ccggcaggtc ttttttcgct
    11641 cacaccggaa ggtgacttac gcgttgatta tcacggctgc ctggagtgtg gcacctgccg
    11701 tttgctgtgc gacgaatcca cactacaaca gtggcgctat ccgccttccg gattcggcat
    11761 cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac ctactccgcc
    11821 agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggct atcgattctg
    11881 aatgccagtt tatttccgtg ttgtacggca atatctgcac catcagtaat atcgttaaaa
    11941 agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa ggcgcgtcaa
    12001 ataaagaagt cgttattcag tttttgaaac tggtgaccga ggcggacggc attatcagta
    12061 ccaaagcctc aatgttgaaa gcggcaagag cggaaggttt tttctgtatt catcgcctgt
    12121 ttattgttga ttcaatttcg tttcacaaca ttgataagca agttgcgcaa tcgaatccgg
    12181 attgtattga gatcctgcca ggcggtatgc ccaaagtgct gggctgggtg acagagaaaa
    12241 tccgccaacc gctgattgcc ggtgggctgg tgtgcgatga agaagatgcg cgtaatgcga
    12301 ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg ggtctggacg ttagcgaaaa
    12361 aattactttg acgggataat cgtaaccaat tgaatttggt ttgatttgta ggccgcacgc
    12421 cacatccgac attcagcgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaagtcc
    12481 ccaggccaga accgtaggca ggatcatccg gcaacacgtt attggcaatc tgcgtgaatg
    12541 tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgccaac atctgcctgt
    12601 gaaaataccg cagcgctggc gtcatgatta atcgccacca caaacttgct attgcgtacg
    12661 cccgccatta acgctgctgc gccagaagcg ccaacaacaa tacacacctc aggtgccagc
    12721 agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat cactcgcgcc
    12781 cgactgtagc ccacttcggc tcccagcttt tctgccagca tagcgatttc ctggttatct
    12841 gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg gtcgcgagta
    12901 acatttttca gatcctctgt gctaaccagc cagtccggaa gcgcacccgg gacaatgttc
    12961 agttgttgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg acgcgccagg
    13021 gaaaggcata gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt tccccagtgg
    13081 gatttgcgca cgcttacggt tggaatatct aacgaagtca cctggcagat gcttgcacca
    13141 tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg cggaaagaga
    13201 acaacgtccg caggcgttcg ctgccactgt tccaccaacg catccagcac ctgttccgcc
    13261 actacaggct gaggttcaat ctgccagtgc gccagggtac aaccgctgaa atcctgcgcc
    13321 gccagccagc ttgctattgc ggcattctcc tgatttattg tcacaattgc gatattcatg
    13381 gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg agctttttct gctaccgttt
    13441 gcccatcaat cagcgtcgct ccgcgtcgtt gctctgctct tgccagttgc agacactgca
    13501 tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc gcagccattc
    13561 gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg gcaatcaccg
    13621 caggcaagcg aacccggcaa caacgcaacc catgttcagt acgctgttcg agggtgataa
    13681 acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc agcatttccg
    13741 ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg gtaattatca
    13801 gatccagcgg attctgatgc tgccattcgg caatatggcg agcgacaaat tccggcgcaa
    13861 agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga gccatgagat
    13921 agcgcagcca gtgcaatgcc cgttcatccc ccatgctcaa cgcggttaaa gacatcggcg
    13981 taccgttttt tctctgcgcc aacagcagcg cggcagcggc ctgttcatca gcaccgagta
    14041 aacttcgcag tagcgaaata tccggtccgc ttttaccctg agccgccgcc tgccactctt
    14101 tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg ttcattcgca
    14161 cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc ccatagccag
    14221 gtcacaacaa aatcaaccaa caacactgtc gccaggagca ataaggtgac ttgcattccc
    14281 cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag tcgactcatg
    14341 gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc cgcaggcaaa
    14401 atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa aagcagcagc
    14461 gttaatgaac tgccggaagg caaacaggcc atcactacca gcgttgccgc cagcagcaaa
    14521 aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa tcccagcagc
    14581 gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc ttccagacca
    14641 atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat tacgaggcag
    14701 acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa caaggttttg
    14761 atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg accaaaatag
    14821 cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca gcgtggtgat
    14881 tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcagagctgc cgatgccagt
    14941 agccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat acttgccagc
    15001 acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg atggcggcgc
    15061 ggggaaaatt cagccagcaa ggtgtgacct actgaataat cgcctcccag accaatgcca
    15121 atcaaaatgc gcagtccaat aagatgctct ggcgtggtgg caaaaaattg taaaaacgaa
    15181 gcaagcgtaa tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat atggtcggag
    15241 atccacccaa gaaccaggct gccaaggaac aaaccaagga gcgccgagcc gccgatcatt
    15301 cccgccataa acggcgtcag ttgcatggcg ggcgtaagct gaataatggc gtaaccaatg
    15361 acgccgagaa cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc aatgcggcag
    15421 tgaaaacggt taagcggtaa atcatccatt cgcaccggtg aagtgttcat ctttacccct
    15481 tcattgtccg gtaataagaa cggcttctcg cctgagaagc cgaaaaggca acgccccgtc
    15541 atcgcggaac gctgcctgac acattatttt tcaatcggat agatagtacc cgtattcata
    15601 atgccattag gatcgaactg ctttttcagc ccttccagca acgcccacgc gctgccgtgt
    15661 tccagtttgc tccagtgaac gcgatgttta ccgataccgt ggtggtgcac catcgaaccg
    15721 ccgaggcgaa tggtttcttc acagatgatc ttgttgagcg gattgtggta cttgtcgatt
    15781 tcctcttccg gcttacagtc aacgacgttg taatcgtaga cgaagtacat gttggtgccg
    15841 ttctgatagc tatgagagga atgaccgccc agcatggtga tgtcgtcggc gtgcgggaac
    15901 tcagtacgaa tacggttaat aacgctttcg tagatttcgt ggatgcagct ccagcagccg
    15961 gacacttcag tggtaaagcc catgttgccg gttttgagga tctgcacacg ttcggcagcc
    16021 actttatccg gtccccagtt caggttgtta aaccaggttt cgatcagctt gctgtccacg
    16081 cgctggcatt gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt cgccttcgca
    16141 atgcgagggt taccttcagc cataaagatc agcacgcatt ttccgtctgc aaaatgggtg
    16201 aagtgttggg tgccatcttc agcgtcatac aaacgagcga tcgacggacg atacccttcc
    16261 accatgactt cacgcaggat gttgaagtcg gttttcatgt cttccaggat atagccgtag
    16321 aagaggttgt tttccggggt gaatttaaag attttcactg ttacttcagt gatatagcaa
    16381 agtgcacctt cgttaccgat gatgatgtgg cgaatgtccg ggccagccgc gcggcgtggc
    16441 acgtttttaa tgcgtgtgac ggtgccatca gccaatactg cttccagacc aacgaccata
    16501 tcttcgattg cgccgtagag tgtggagaac tgcccgatac tgcgggttgc taccaagccg
    16561 cccatctgcg ccagcggcct tgactgcgga gaatgccccg tggtgtaacc tttttcacgc
    16621 aacgcatttt ccagcacctc cagcggaacg ccacattgcg ccgtcgcctg catattctca
    16681 atatcaatat taatgatttg attcatggcg gagccgtcaa gcaccaccga gttttccaca
    16741 acggtttcca gcccaccttc agtggcggaa gcgccggtac gcggcacacc gttaattttg
    16801 tgcgcattca taaaattcag cacacgggat acttgctctg tggaaccgag ttttacgacc
    16861 gctgccggaa tcggcaaagt ataaatgcca tgaatatccg gaaatttacg aaaacggtca
    16921 atactgttct tctttaatac tgtttcatcg gtaattacgc gatctgcacc aacaatttcc
    16981 tttagttggt cgacaatcgc tgcgcgagat aaagacatag taattccttc ctgataataa
    17041 aaatggaata atcattaata agagaaaaca gaaaccaccg ttatggatta gcgcactaaa
    17101 taaccaccat tgaccactaa taaatgccca ttgacataat ttgaagccgg acttgcgagg
    17161 aatacagctg cgcccattaa atcctgagta tcgccccaac ggtttgccgg aatatgatca
    17221 agaacgcgct gattggtttc tggattactg cgtgtcgcca gcgtaatatc tgttgcataa
    17281 tagccaggag caataccatt tacctgaata ttatattgac ctagttcatc acaataagct
    17341 ttggtgaatc cggcaagagc atgtttagtg gcagaatatg caggtgacca ttgtccacct
    17401 aagtaagaga acaatgaaca gatattaata attttgccgc ttttctgcgg gatcataatt
    17461 tttgcagctt catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat catcggatcc
    17521 cagtcggcac gaccgaagtc cagcacctta ttcagcttac aaataccggc attgtttacc
    17581 agaatatcaa ctgtaccgaa acgctcacag caagcagcga taatcttctg cggcgcgcct
    17641 tctgcggtga tatccacctg catgaagtcc acctcaacac cctgtttttc aatcatttcc
    17701 tttgtttcgc cgttatcttt caatcatttc ctttgtttcg ccgttatctt tgacgaaact
    17761 aggaataaag atatttgcgc cagctttggc caacgccatg gcaaatgcct ggcctaaacc
    17821 gctattccca ccggtaacaa ttgcggtttt acctttcagt gagaaaaaat ccattgagaa
    17881 cgcattgaga gattcgattg acatagttag ctccaatttc cgtaaggcaa aaaaaagaaa
    17941 ggtaacttcc cccgcgagag ggaagttacc tttctcatta tctctggtaa ctggagataa
    18001 tttttagcat aacggcataa ctgctaatgt gatcgtaatc acagtgtgat attcgtattg
    18061 tcaaatagtt ttaaaaagca gaagcgtgat tactctcaca caacacctct gctttccatg
    18121 ttatttctta ttgcaattac tagagacctg agaaaagtaa tccacctgaa agggcggctt
    18181 acttttctct tttttttgtc tattacaaat gggaatacga tatgcaacac aactcatatc
    18241 gccgttggat aaccctcgcg ataattagtt ttagcggcgg cgttagtttt gacctggctt
    18301 atttacgtta tatttttcaa attcccatgg cgaaatttat gggattcagc aataccgaga
    18361 taggtttaat aatgagtacc tttggtattg cggctattat tctttatgcc cccagcggcg
    18421 ttattgccga taaattttca caccgcaaaa tgattacttc cgcgatgatc attaccggat
    18481 tactgggtct gttaatggca acgtatccac cgctgtgggt aatgctctgt attcaggtcg
    18541 cctttgcgat aacgacgatt ttaatgctgt ggtcggtgtc gattaaagcc gcctcgttgc
    18601 tgggcgatca tagtgagcaa gggaaaatta tgggctggat ggaagggctg cgcggcgtcg
    18661 gtgtaatgtc gctggcggtg tttaccatgt gggtcttttc tcgctttgca ccggatgaca
    18721 gcgccagcct gaaaacagtc attatcatct acagtgtggt ttacatcttg ttggggattc
    18781 tgtgctggtt ttttgttagc gataacaaca acctgcgcag tgccaataac gaagaaaaac
    18841 agtcattcca gcttagcgac atccttgccg ttttgcgtat cagcaccacc tggtattgca
    18901 gcatggtgat ttttggtgtc ttcacaatct acgccattct gagttactcc accaactatc
    18961 tgaccgaaat gtacggcatg tcgctggtgg cagcgagcta catggggatt gtgatcaaca
    19021 aaatcttccg cgcgctgtgc ggcccacttg gaggcattat caccacctac agtaaagtga
    19081 aatcccctac ccgcgtgatt caaatccttt ccatcatcgg tttgctggcg ttgacagccc
    19141 tgctcgtcac gaactctaac ccccaatcgg tcgcgatggg gattggcctg attttactgc
    19201 tgggattcac ctgttacgcc tcacgcgggc tgtactgggc ctgccccggc gaagcgagaa
    19261 caccgtctta cattatgggc accacggtag gtatttgttc ggtgattgga ttcctgccgg
    19321 atgtcttcgt ttacccgatt atcggccact ggcaagacac cctgcccgct gcagaagcct
    19381 accgcaatat gtggctgatg ggcatggcgg cgctcggcat ggtgattgtc tttacctttt
    19441 tgctgttcca aaaaattcgt actgctgata gcgcccccgc aatagctagc agcaagtaag
    19501 ccaggaaaat tacaggagaa aaatcatgtc gaaaaaatac atcataggga ttgatggcgg
    19561 aagtcagagc acaaaagtgg tgatgtacga tctggaaggt aatgtggttt gcgaaggtaa
    19621 aggcttatta cagccgatgc acacgcctga tgccgatact gcagaacatc ctgacgacga
    19681 tttatgggca tcattatgtt ttgcaggtca cgatttgatg agccagtttg ccgggaataa
    19741 agaagatatt gttggcatcg gcctgggctc catccgttgc tgccgtgcgt tattgaaagc
    19801 cgacggcacg ccagctgcgc cgttgattag ctggcaggat gcacgcgtta cacgccctta
    19861 cgaacatacg aatcctgacg tggcgtatgt aacctctttt tcgggttatc tgacgcatcg
    19921 cttaaccggc gaatttaaag ataatatcgc caactatttt ggtcagtggc cggtggatta
    19981 taagacctgg gcatggagcg aagatgccgc agtcatggag aagtttaata tcccccgtca
    20041 tatgctgttt gatgtgcaaa tgccaggcac tgttcttggg catatcacac ctcaagccgc
    20101 gctggcgacg catttcccgg caggactgcc ggttgtttgt accaccagtg ataaaccggt
    20161 agaagctctg ggagccggat tactggatga tgaaactgcg gtaatttctc ttggcactta
    20221 catcgcattg atgatgaacg gcaaagcctt gccgaaagat ccggtggcgt actggccgat
    20281 tatgtcttct attccgcaaa cattgctgta tgaaggttac ggtattcgca aaggcatgtg
    20341 gacggtgagc tggctgcgcg acatgttagg cgagtcgtta attcaggatg ccagggcgca
    20401 ggatctttca ccggaagatt tactcaacaa gaaagcctct agtgtgccac ctggctgtaa
    20461 tgggctgatg acggtgctgg actggctgac caatccgtgg gaaccgtaca aacgcgggat
    20521 tatgatcggc tttgattcca gcatggatta cgcatggata tatcgttcga tattggaaag
    20581 cgtggcgctg acgctgaaga acaattacga caatatgtgt aatgaaatga atcactttgc
    20641 gaaacatgta atcattactg gcggcggttc gaacaggatc tgtttatgca gatttttgcc
    20701 gacgtgttca accttccggc acgccgtaac gccattaacg gttgtgcaag cctgggggca
    20761 gcgattaata caggggtagg tctggggcta tacccggatt acgcaacggc tgtcgataaa
    20821 atggttcgcg tgaaagatat ctttataccg attgagagca atgccaaacg ctacgacgcg
    20881 atgaataaag gcattttcaa agacctaacc aaacatactg atgtgatcct gaaaaaatcg
    20941 tatgaagtga tgcatgggga attggggaat tggggaatgc ggattcgatc cagagctggt
    21001 cgaatgcgtg aataaggtag gccgggcgga aaactgcccg gcctgaacat acctgaatgg
    21061 ttatccccgc tggcgcgggg aacataagtt cgacaactta aaaaatgata acaagtccgg
    21121 tttatccccg ctgatgcagg gaacataata aaaactttaa ctagttcggt ttatccctgt
    21181 ttttacaagg aat
//
LOCUS       DAJTVK010000070        21005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000070 DAJTVK010000000
VERSION     DAJTVK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.070"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..172)
                     /locus_tag="OO372_002590"
     CDS             complement(<1..172)
                     /locus_tag="OO372_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342050.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="OO372_002591"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="OO372_002591"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6342051.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="OO372_002592"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="OO372_002592"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6342052.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="OO372_002593"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="OO372_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6342053.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="OO372_002594"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="OO372_002594"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6342054.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="OO372_002595"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="OO372_002595"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6342055.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="OO372_002596"
     CDS             4290..5153
                     /locus_tag="OO372_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6342056.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="OO372_002597"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="OO372_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6342057.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="OO372_002598"
     CDS             6491..7108
                     /locus_tag="OO372_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6342058.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="OO372_002599"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="OO372_002599"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6342059.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="OO372_002600"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="OO372_002600"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6342060.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="OO372_002601"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="OO372_002601"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6342061.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="OO372_002602"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="OO372_002602"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6342062.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="OO372_002603"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="OO372_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6342063.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="OO372_002604"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="OO372_002604"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6342064.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="OO372_002605"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="OO372_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6342065.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="OO372_002606"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="OO372_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6342066.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="OO372_002607"
     CDS             18074..19783
                     /locus_tag="OO372_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6342067.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="OO372_002608"
     CDS             complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="OO372_002608"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6342068.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5713 c         5387 g         4822 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 actctacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgacg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ttgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattctgcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaat ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagct
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg tatgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atctcgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccaga
//
LOCUS       DAJTVK010000071        20711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000071 DAJTVK010000000
VERSION     DAJTVK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.071"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO372_002609"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO372_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6342069.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO372_002610"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO372_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6342070.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO372_002611"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO372_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6342071.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO372_002612"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO372_002612"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6342072.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OO372_002613"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OO372_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6342073.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OO372_002614"
     CDS             complement(8214..9107)
                     /locus_tag="OO372_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6342074.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO372_002615"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO372_002615"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6342075.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO372_002616"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO372_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6342076.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OO372_002617"
     CDS             complement(10238..11470)
                     /locus_tag="OO372_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6342077.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO372_002618"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO372_002618"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6342078.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO372_002619"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO372_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6342079.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="OO372_002620"
     CDS             complement(14275..16539)
                     /locus_tag="OO372_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCR6342080.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO372_002621"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="OO372_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6342081.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO372_002622"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="OO372_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6342082.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO372_002623"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="OO372_002623"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6342083.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO372_002624"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="OO372_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6342084.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4970 a         5732 c         5045 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAJTVK010000072        20547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000072 DAJTVK010000000
VERSION     DAJTVK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.072"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            753..1181
                     /gene="uspC"
                     /locus_tag="OO372_002625"
     CDS             753..1181
                     /gene="uspC"
                     /locus_tag="OO372_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR6342085.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="OO372_002626"
     CDS             complement(1188..2612)
                     /gene="otsA"
                     /locus_tag="OO372_002626"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6342086.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="OO372_002627"
     CDS             complement(2587..3387)
                     /gene="otsB"
                     /locus_tag="OO372_002627"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6342087.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(3554..4540)
                     /gene="araH"
                     /locus_tag="OO372_002628"
     CDS             complement(3554..4540)
                     /gene="araH"
                     /locus_tag="OO372_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6342088.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            complement(4555..6069)
                     /gene="araG"
                     /locus_tag="OO372_002629"
     CDS             complement(4555..6069)
                     /gene="araG"
                     /locus_tag="OO372_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6342089.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(6139..7128)
                     /gene="araF"
                     /locus_tag="OO372_002630"
     CDS             complement(6139..7128)
                     /gene="araF"
                     /locus_tag="OO372_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCR6342090.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            7925..8428
                     /locus_tag="OO372_002631"
     CDS             7925..8428
                     /locus_tag="OO372_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6342091.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(8507..8758)
                     /locus_tag="OO372_002632"
     CDS             complement(8507..8758)
                     /locus_tag="OO372_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6342092.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="OO372_002633"
     CDS             complement(8873..8959)
                     /gene="azuC"
                     /locus_tag="OO372_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6342093.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            9222..9545
                     /locus_tag="OO372_002634"
     CDS             9222..9545
                     /locus_tag="OO372_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6342094.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            9716..10213
                     /gene="ftnA"
                     /locus_tag="OO372_002635"
     CDS             9716..10213
                     /gene="ftnA"
                     /locus_tag="OO372_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6342095.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(10251..10490)
                     /locus_tag="OO372_002636"
     CDS             complement(10251..10490)
                     /locus_tag="OO372_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6342096.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            10681..11892
                     /gene="tyrP"
                     /locus_tag="OO372_002637"
     CDS             10681..11892
                     /gene="tyrP"
                     /locus_tag="OO372_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6342097.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="OO372_002638"
     CDS             complement(11954..12619)
                     /gene="yecA"
                     /locus_tag="OO372_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6342098.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(12815..12901)
                     /locus_tag="OO372_002639"
     tRNA            complement(12815..12901)
                     /locus_tag="OO372_002639"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12865..12867),aa:Leu,seq:taa)
     gene            complement(12914..12987)
                     /locus_tag="OO372_002640"
     tRNA            complement(12914..12987)
                     /locus_tag="OO372_002640"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12953..12955),aa:Cys,seq:gca)
     gene            complement(13042..13117)
                     /locus_tag="OO372_002641"
     tRNA            complement(13042..13117)
                     /locus_tag="OO372_002641"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13082..13084),aa:Gly,seq:gcc)
     gene            complement(13269..13817)
                     /gene="pgsA"
                     /locus_tag="OO372_002642"
     CDS             complement(13269..13817)
                     /gene="pgsA"
                     /locus_tag="OO372_002642"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6342099.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(13874..15706)
                     /gene="uvrC"
                     /locus_tag="OO372_002643"
     CDS             complement(13874..15706)
                     /gene="uvrC"
                     /locus_tag="OO372_002643"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6342100.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(15703..16359)
                     /gene="uvrY"
                     /locus_tag="OO372_002644"
     CDS             complement(15703..16359)
                     /gene="uvrY"
                     /locus_tag="OO372_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6342101.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            16655..16831
                     /locus_tag="OO372_002645"
     CDS             16655..16831
                     /locus_tag="OO372_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342102.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            16818..17042
                     /locus_tag="OO372_002646"
     CDS             16818..17042
                     /locus_tag="OO372_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6342103.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(17109..17831)
                     /gene="sdiA"
                     /locus_tag="OO372_002647"
     CDS             complement(17109..17831)
                     /gene="sdiA"
                     /locus_tag="OO372_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR6342104.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(18061..18813)
                     /gene="yecC"
                     /locus_tag="OO372_002648"
     CDS             complement(18061..18813)
                     /gene="yecC"
                     /locus_tag="OO372_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6342105.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(18810..19478)
                     /gene="tcyL"
                     /locus_tag="OO372_002649"
     CDS             complement(18810..19478)
                     /gene="tcyL"
                     /locus_tag="OO372_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6342106.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(19493..20479)
                     /gene="dcyD"
                     /locus_tag="OO372_002650"
     CDS             complement(19493..20479)
                     /gene="dcyD"
                     /locus_tag="OO372_002650"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6342107.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         5254 a         5227 c         4729 g         5337 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta atgttaatga aatgttttac
      541 atacattaac cacttgttta atgcggagaa tcagtacact attttcttat aattacattt
      601 gaaattatat gatcaccggg agtgattgat taattgtctt tactaataat cagactaata
      661 tttacctgtt tgaccgagtt gggattgcgt cgtttctcca ttaggagtaa agctttaatg
      721 tcacctgaag ttcacagaat aaggaacagg ttatgagcta tagcaatatt cttgtcgctg
      781 ttgcggttac accggaaagt cagcaactgc tggcaaaagc ggtatctatc gccaggccag
      841 taaagggaca catcagttta attactctcg cttccgaccc ggaaatgtac aatcaattag
      901 ccgcgccgat gcttgaagat ttacgcagcg tgatgcaaga agaaacgcaa agctttcttg
      961 ataagttcat tcaggatgcg gggtatcccg ttgacaagac gtttattgcc tacggtgagt
     1021 taagcgaaca tattctggaa gtatgtcaca aacatcattt cgatttggtg atttgcggta
     1081 atcacaatca cagtttcttt tcgcgagcat cctgctcggc gaaaagagtg attgcctcaa
     1141 gtgaggtcga tgtgctgtta gttccactta cgggagatta accgctccta cgcaagcttt
     1201 ggaaaggtag caactttatc gcgctgctgg ctttccgcgc ttcgcggaac tatctgcttt
     1261 aggtcgctaa tgaagcactc ctgccagtgg ttaatatcgt ttttcacgat aacgtccagc
     1321 atttctgcat gacgggaaat acgttccgcc agcgacatag tcaatgcacg atccagcgca
     1381 gctgcaactt cgtcacgatc gtaggggtta acaattaacg ccgacgttaa ctcgtttgcc
     1441 gctcccgcaa attgcgaaag aacaagaacg cccgggttgg ctgggtcctg agcagcaaca
     1501 tactcttttg ctaccaggtt catcccgtca cgcagtggcg tcactaagcc cacgtcagag
     1561 tagcggaata ttttcatcag taatttacgg tcaaaatgct gattcaaata ataaagcggc
     1621 gtccagccta attgcccgta tttaccatta attcgtccag cttcattttc gagctgatga
     1681 cgaatatcct gataggcttg cacatcacca cgcgacgttg gtgcaatctg ggtataacga
     1741 attttaccat gatgctgcgg atatttttcc agcaacgctt cataggcgag aaaacgctct
     1801 ggcaaacctt tggaataatc cagccgttcg acagaaaaga tattttgtac gtttttcagt
     1861 tccgctttaa gttgcgccag ttttggcggc agtggcccgg cagcctgttt ggctatttct
     1921 ttcggttcaa tgccgatcgg gtagacttct gttcgaaatg ctttgcccca ggctgtatgg
     1981 ctttttgcgc tacgtgtcgt gacgcgggtc aggttagaaa gacaatccag gaacgccaga
     2041 cgatcgtttt ctgtctggaa acccagcaaa tcataatcac aaagctgttc aagcaaggtg
     2101 tcatatgtcg gcagcgcgtt gaagatttcc ggtgtcggga aaggaatatg cagaaagaaa
     2161 ccaatgcgat tattcactcc ccgtttgcgt aattcatgcg caaatggcaa caggtgataa
     2221 tcgtggatcc agataatgtc atcgtcttgc aacagcggca gtaatttatc tgccagcaac
     2281 gcatttacgc gtagatagcc gtcccaggca ggacgctgaa attgcaccag atcgagccga
     2341 taatgaaaag cgggccagag aacggcattg gagaattggt tgtagtattc gtcaaggtcc
     2401 tgttcgctga ggttaaaaga ggcccacgta atgttacctt ttttcacctt ttttagcggc
     2461 tgatcctcat tccctgtttc accactccag ccaaaccaca gtccgcctgc ggctttcagt
     2521 gcccccagta tgccaacggc aaggccaccg gcactggcgg cgtgctcgtc tggtggtgca
     2581 atccggttag atactacgac taaacgactc atagtcatca ctcctgttat tttctctttt
     2641 ttgttgtaat gcggtggtta tcatttcaag ccagctccag acatccggca cacccgccag
     2701 tcgccatgat gcctgagttg cacctgtgcc aatttttact gacattccgc ccagtcggtt
     2761 aacgactgcg aagccagatt catcggttaa atcatcgccc agaaatacgg gcgttcgccc
     2821 gataaaggga gcttcctgca taaaagctgc aattgcctca cctttactgg tacctctcgg
     2881 tttgatctcg acaacacact ttccctgctg taacgccatt tgtggccaga tctgagtaat
     2941 acgttgcgct aatgtcatta atgcgtcttc atgctgcgga gcctgacgat aatgcagcgc
     3001 aaaagccatc cctttcgcct ccagctccgc gccgggatac tgagcgatga ctgtatgcag
     3061 ttgcacgcta atatcacgcg caatcgcatc cggcagatga acgatatgtg ttttaccatt
     3121 gatgtcacgg cgctccgccc catgcacgcc cgctaacggg aagcgataag gttttgccag
     3181 tgcgtcaagc tccaccattg agcgccctga tatcaatgcc aatgcaccat cacttgcggt
     3241 tgccagtagc tgtagtcctt gcagaatatt gtcaggcacg acgacctgat cgggatgcgg
     3301 tttgatttcc gccagcgttc catcaagatc aaaaaaccag gcatatttcg cggatagttc
     3361 aggggtttcg gttaacggtt ctgtcacccg gctctcctcc ttctcttttc ttattgtttt
     3421 gcgcagaacg cgctttacag acatgtaagt atagacagtg tgacgggggt cgccatttgc
     3481 aatgaagaac agccaaccgt tgtggcggct ggctgtggtg ggaaaaaacg ttaaattgtt
     3541 gtggaaaaaa gcatcagaca atgcgtttcg ctttttgctt gtaacggtcg aagatcaccg
     3601 ctgccagcag gattaagccg cgaaccacgt actgcgcgaa aggagaaata ttaagcaggt
     3661 tcatggcgtt ttccacggtg cctaaaatta agatacccgc caccacatat gagatttttc
     3721 cgatgccacc tttcagagaa acgccaccta aaacgcaggc ggagataaca ataagctcat
     3781 aaccaatcga cgtcattggc tgcccactag tcatacgtga agccagaata attccggcta
     3841 tcgcagatac caggccagag agaacaaaga taataatttt ggtgcgaaca accggtacac
     3901 ccgccagacg cgcagcctct tcgttcccgc caatcgccag ggtgttacga ccaaaggtgg
     3961 ttttattcag caacaaacca aagataatca gacacgcgac ggtgagccag attggcgcag
     4021 gcagaccgaa ccagttagcg taaccaaggg caaagaagct ttcatcttcg ataccgaccg
     4081 ctttaccgtc tgaaatgatg tacgccagac ctcgaacaat ctgcatcgtt gccagtgttg
     4141 tgatcagagc atttattttc agtttggcga taacaaagcc attgaccagg ccacagagaa
     4201 tgccaagcag caaccccgct gccacgccaa tccacaggct ttcagtcagg ttgataacca
     4261 ccgccgtggt gacacccgca caggcaatta cggaggcgac agaaaggtca aagtcaccgg
     4321 aagcaaggca gaacaacatg ccacacgcca ccatccccga catggaaatt gccaggccca
     4381 accctttcat attaatgaag gtggcaaaat ttgggacaaa aatggcacag gcgataaaga
     4441 gcaccgcaaa caccaccagc atgccgtact gatcccagat acgcccgaag ctgaatgacg
     4501 acttaggtgc gccagaaccc gatgtagaaa cagaagacat catactctcc ttactcaggc
     4561 aactgcctgg ctgactttag gcatcgcaag gctcagtgcc tgacgctcat ctgcctgctc
     4621 gtgtaacaat tcaccggcga tttcaccttc ccgcatcacc acaatccggt cggcaacgcc
     4681 gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc acaccctgcg ccgccagcgc
     4741 ataaattacg ttatatattt cgtgcttagc accaacatca atgctgcgcg taggttcatc
     4801 cagcaaaatg accttcatct cttccgataa ccagcggccc agaatggctt tttgctgatt
     4861 tccgcctgag agattcatga tcagttgctc agcgcccggc gttttgatgt tgagcgaacg
     4921 aatgtgctga tcggcattgt tttcttccca accgttgttg attacacaac cgccgagcac
     4981 atgtttgcgt ctggcactga tgttgatatt gtcgcgaacg gagtgcacgg gaataatgcc
     5041 ttccgctttg cgatcttccg ggcagagcat catgcctgcg gcaatggcgt ggctcggttt
     5101 acgaatatcg atcggctgtt ggtcgatata aacctgaccg gcggtgattt gcgtcccgcc
     5161 aaacaagcct ttcattaatt cgctacgccc cgcccctacc agaccaaaca gcccaacaat
     5221 ttcaccactg cgaaccgcca gacttattgg cgtacgcacg cctggtgctt tcacagcatc
     5281 aagacgcagg cgctcctcgc cataactacg aggttgccag ccgtagatat cgccaatgtc
     5341 gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc tgctgcatat cggtaaaggt
     5401 tttgacataa cgtccatctt taaagacagt gatggcatcg ctgagggcaa atatttcttc
     5461 catacggtga gaaacgtata agatcacccg cccctctttt cgcagttcac gaataacgcg
     5521 gaaaagattg tcgatttcgc gggcagaaag ggagctggtt ggctcatcaa aggcgataat
     5581 tttagcgtta cgcgccagcg ccttggcgat ttcaaccatc tgccactgac caatggagag
     5641 atatttcagc ggcgtgtccg ggtcaatatc cataccaaga tgtttaagtt gtaaacccgc
     5701 ctcataattc agcaatgagc gattcacaat gccgccttta tgcggcagct ggccgagata
     5761 gatgttttcc gcgacggtca tttccggcac gagatgcagt tcctggtaaa taatcgccac
     5821 acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt tcctgcccat taatcactac
     5881 agaacccgtg gttggcgcat agttgccgct gaggattttt aagagagttg attttcctgc
     5941 gccattttca cccatcaacg catgaacctg accggcatag cagtcaaaac taatatccgt
     6001 cagcgcctta acgccgggaa acgttttacc gatgccgcga aatgagagat acggggtaga
     6061 ctgttgcata acgtctccgt gaatcactgg tcgttcacac cccctctgca tcatgcagag
     6121 gggaattttt ccggcaaatt acttaccgcc taaacctttt ttctccagtt cttctttaaa
     6181 gttgtcacgc gtgatcagca ccacgtcggt aacttcggta aattttggcg gttcaacgtc
     6241 ttttgctacc cagttgtaaa gcatttcgct ggatttatag ccatgtacgt ccgggcttgg
     6301 cagcagggaa ccgtagaagc cggttgcctg tgctttagac agttcgctca acgcatccac
     6361 accgttaatg ccaatgccga tgatatcggc agctttaaag ccctgacctt ccgtcgcgcg
     6421 tacgccgccc agcacggtgc tgtcgttcat accgacgatc agccaatgtt taacttccgg
     6481 atgttgaacc agcattgagt tggcagcgtc aaatgccccc gggatgtcgt tagatttggt
     6541 aggtacctga taaatttgtt tttccgggaa tccggccgct ttcagcgcat ccatagaacc
     6601 cgttgtacgg cgacgtgccg tatccagttc gttggcggta atcgccatca ccgcgctttc
     6661 tttgacatcc cagccacgtt tctgcatctc tttatacagt tcctgaccct gacgttcgcc
     6721 aattttagtc gccgccatca tcaccagcgg aacggtatcc attggcttac ctttggcgtt
     6781 aacaaactgg tcatccacgg caatgacttt catatcgtag ccacgcgctt tcgcgacgat
     6841 ggcagagccg agtttggggt ccggagtaca aataacgaaa ccttttgcgc cactggcagc
     6901 caggctgtcg atcgcgttca atgttttttc gccatccggc acggcaatct taataacctc
     6961 aaaccctaaa tccttcccgg ctttatcggc aaacttccat tcggtctgga accacggctc
     7021 ttccggttgc ttcaccagaa aaccgagctt caggttctcc gccatagcgg attgtgacat
     7081 aacggctgcc agaccaatgg ctgccagggc tttagtaaat ttgtgcatgg ttctctccag
     7141 ctttagtgtc gttttgtgta gggcaaaaac gaatgacttt cgttaaatta atcggaaaac
     7201 aaagcattac cttttatcta aaagataagt gaccgtgttg acatagtttt agcgagaaat
     7261 taattctcca taggagagca atatcacatc gcagaattac agtgagaacg tgcataaatt
     7321 tagcgggaaa agacataagg gaaagccaat ttgtcagaca aattgtcgaa tgcacagcag
     7381 attaatccat aagattagcc tggaaatcct tgttgtcttt ggtacccatg cgggatgtct
     7441 tctttttaac cagtcaatag gccgcattac ctggcgttga gtttttgaaa tggtgtaata
     7501 accgcaactc aaagatgtgg aaaatgcacg tcattcattt cgccattaat tatcactgtg
     7561 ctcattaatt aacagaacac gtataatgag agccatctcg caaaaatgaa aaaacgtttt
     7621 ataaaatcat cacttcatca tgaattcaaa ttcattgatt aatatcaaca agatacaaaa
     7681 agcactatca ttaaaattca ttgcagttac attgatttca tcaatgaaat gtaaaaatat
     7741 ataaacttga tgatttaagc attttcttat acccgttcag acgttattct tatttcagat
     7801 catcgtcaga attgactcca cgatcacatt tcggaccggc agaaaggaat tattctgcaa
     7861 acagtaatta tggtgttttg atttatcttg cacctctcca cttctggata taaggatatt
     7921 aggtatggca accgctggaa tgcttctcaa actcaactct caaatgaacc gcgagtttta
     7981 cgcatccaat ctctaccttc acctgagtaa ctggtgttct gaacagagtc tgaacggcac
     8041 cgccactttc cttcgcgccc aggcacagag taatgtgacc caaatgatgc gcatgtttaa
     8101 ctttatgaag agtgtcggcg ctacccccat cgttaaagcc attgatgttc ccggtgaaaa
     8161 actgaactct ctggaagaac tgttccaaaa aacgatggaa gaatacgagc aacgttctag
     8221 tacgttggca cagttagccg atgaagcgaa agaactgaat gatgattcaa ccgtcaattt
     8281 cctgcgcgat ctggaaaaag aacagcagca tgatggtctg ttgctgcaaa ccattcttga
     8341 tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg cagaccgacc aacatgttct
     8401 gaatgtcgtg tcacaccagc tgcattgatc atcatcggcg ctaatgcatt gcgccgatga
     8461 aggttttgag aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac
     8521 cctgtggggt aaacttgagt tcaataagcg cgatggcttt ttggattgcc cgcatggtga
     8581 ccgggtctgc ggcggcggga tggttagtaa agtcgatatt cttcagctga ctggacattt
     8641 tttcacgaac ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca
     8701 actggcaagc aaccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg
     8761 taaactcctg atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag
     8821 tcacatccac cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga
     8881 acatattgcc tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca
     8941 ggattttgcg cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt
     9001 tgcgcttata atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa
     9061 ataccccttt aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct
     9121 acagatggca agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta
     9181 acgcgtaaaa tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga
     9241 ctctttcatt aataacgctg gcggggtgta cggttactcg ccaagcccat gtgagcgaag
     9301 ttgacgcggc aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc
     9361 ataccgatcg ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata
     9421 cccacgcggt cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat
     9481 gtttgaatac ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc
     9541 tctaaatatt ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt
     9601 ttattcccat tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa
     9661 ataacaaatt atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct
     9721 gaaaccagaa atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact
     9781 gctttatcag caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt
     9841 cctgcgccgt cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac
     9901 tgataccggc aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc
     9961 acttgatgaa ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa
    10021 cgaactggct catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg
    10081 gtatgtttct gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag
    10141 cctggcaggc aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga
    10201 cacacaaaac taatgctctc cggcggcagg tttactgccg ccgtttcaaa tcagtgacgg
    10261 caaatcttac tctgatcggt cgaaaaaccg tctttcgaag gtataaattt gccttttgct
    10321 gccagaaacg ctaccagctc tcccgctgtc atcccttctg ccgagcaggt gtgaaaacgt
    10381 gcctgttcac caaaacgcgc tttaatcgca gcttccagac tggcatgcgt atattgctcg
    10441 cctgattcaa tcatcatatt taacacttca tgaccgtgaa tagagtccat cgttcctcct
    10501 caaaaaaagc ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcaggactga
    10561 atgcttttta ttgtacattt atatttacac tatatgtaac gtcggtttga cgaagcagcc
    10621 gttatgcctt aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc
    10681 gtgaaaaaca gaaccctggg aagtgttttt atcgtggcgg gaaccacaat tggcgcaggc
    10741 atgctggcaa tgccgctggc tgcggccggt gttggtttta gcgttacgtt aatcttgttg
    10801 attgggcttt gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat
    10861 gttccggcag ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcaa
    10921 tggctgacgg gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc
    10981 ggtgtcggtg aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca
    11041 accgctggcg tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt tggaacatcg
    11101 ctggtcgatt tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg
    11161 ctggtattgc tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag
    11221 gggctggctc tgtcagcaat cccggtgatt tttacctcat ttggttttca cggtagcgtg
    11281 ccgagtattg tcagctatat ggatggcaac gttcgtaagc tacgctgggt gtttataacc
    11341 ggtagtgcga tccccctggt ggcatatatt ttctggcagg tggcaacgct tggcagcatt
    11401 gattcaacaa cctttatggg attgctggct aatcatgctg gattaaacgg gctgttacag
    11461 gcgttacgcg aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat
    11521 ttagccctcg ccacgtcatt tctcggcgtt gcgttaggct tatttgatta tctggctgat
    11581 ttatttcagc gttcaaatac cgttggtgga cggttgcaaa ctggtgcaat tacctttctg
    11641 ccgccgttgg cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc
    11701 ggtgtggcgc tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcaga
    11761 aagcacaatc ctcaggcggg ttaccgggtc aaaggtggtc gtccggcgct ggtggtggtg
    11821 tttctctgtg gtattgctgt gattggcgtg caatttttga ttgcggcagg gttgttacca
    11881 gaagtggggt gatcagatag cctcaaattc cttattgggt gccagaatta acgctgacac
    11941 ccaatttggc ctcttaatgc aggcagcact gcttaaattt cttaccacta ccgcacgggc
    12001 aaggatcgtt acgccccggt ttctcttctg ctttgatcgg ttgctgaaca gctttttcct
    12061 gcggatgcgc catccagtac gcatgtagat caagcgccgc cagtcgaatg gcatctacgc
    12121 tctcttcaaa cgcttctggc gacatctttt ctacccgctc gaagttttcc tcagtaccgt
    12181 gcagcgcaat cgcctccagc gctggtttta acgaatcggg caacgttgac cagtcagaaa
    12241 gtgccacgcc ccgcatatag ccaaagcacc actcctcaac aatcgtcagc tcgctgccat
    12301 caacttctcg caagccgaat aacggctcaa actgctccgg gaattcgttc agacgctctg
    12361 cggtatcggc catatgttga aaagccaggt tcataaagcg cgtcatctct ttctctgacg
    12421 cccagcgcgg cacatagtca gccccacccc acacggcaac cagccactgt tccggttcaa
    12481 tctcttgcgg agaactcaac accgccgtca ataaaccgtc cagctccgcc acatcaagga
    12541 tggcgtggtt agtgttgtat ttggtcagaa tatcgtccag ccattccaac tcactttcgt
    12601 ttaacggtcc cgttttcata cgcttttcct tgtggatctc aactcgccag cacctatctt
    12661 acatgccggt ccgtatcaga gatacttttt gagtggcttt gccggtgatt aaaaattaag
    12721 gagggtgtaa cgacaagttg caggcacaaa aaaaccaccc gaaggtggtt tcacgacact
    12781 gcttattgct ttgattttat tcttatcttt cccatggtac ccggagcggg acttgaaccc
    12841 gcacagcgcg aacgccgagg gattttaaat cccttgtgtc taccgattcc accatccggg
    12901 ctcgggaaga aagtggaggc gcgttccgga gtcgaaccgg actagacgga tttgcaatcc
    12961 gctacataac cgctttgtta acgcgccaaa ttcttcaggc ctttcagcca gacatccgct
    13021 tgacgccgat gtcttttaaa ctggagcggg aaacgagact cgaactcgcg accccgacct
    13081 tggcaaggtc gtgctctacc aactgagcta ttcccgcatt catcaagcaa tcagttaatc
    13141 acttgatttt attatcgtct ggcaatcagt gccgccgttc gatgcgttgc attctactta
    13201 cctggcgcga tgagtcaacg atatttttca ccacttttga tcgtttgctg aaaattacgc
    13261 cgaaacgatc actgatcaag caaatctgca cgcgcagcgc tcaaatattg caacattgac
    13321 cacagagtca gtaccgcagc cacaaagaaa agtgcaatac cggcgtactc aacccaaatg
    13381 ttcggacgcc acagcagcca tgccaacgcc accatctggg cagtggtttt cactttccca
    13441 atccaggaga cagccacgct actgcgttta cccaactccg ccatccattc gcgtagcgca
    13501 gaaataataa tttcacgggc gatcatcgtt gctgccggta aggtcaccca ccagctgtgg
    13561 taatgctcgg ttaccagcac catggcgata gccacgagaa ctttatctgc tacagggtca
    13621 aggaaagcac caaaccgggt actctggttc cagcggcgtg ccagaaaacc atcgaaccag
    13681 tcagtcaccg ccgcgacgca gaaaatgagc gcggcggcaa acggcgacca ggtgacaggc
    13741 agataaaaga ccaatacaaa gaatgggata aggatgacac ggaacagtgt aagcaacgta
    13801 gggatattaa attgcataat gacgggtaac tatctgttgt cagtaagatt acccctatgt
    13861 tgctacagag acatcaatgt ttcaacgacc agaagatctt ttctgccaga ccttgcgaaa
    13921 tacccggcac ttttgcaatt tcctcgacgc tggcgttacg taaaccttgc aaaccgccca
    13981 tatatttcaa caacatttgc cgacgttttg gcccgacgcc ttcaatggtt tccagggaac
    14041 tggtattttt gaccttcgcc cgttttttac ggtgcccgcc aatcgcgtga tcatgtgatt
    14101 catcgcgaat atgctggata acatgcagcg cgggagaatc tggcggcaaa ctgaatccct
    14161 caccttccgg ctcaaagaac agcgtttcca gcccagcctt acgatctgct cctttggcaa
    14221 cgccaagtag cagcggatga tttttatccc atgagacatc cagttcggcg aagacatttt
    14281 tcgcctgcgc aagctggcct ttgccgccgt cgataaggat cacatccggg atcttactgt
    14341 cgtcaatggc tttaccataa cgccgacgca gcacctgatt catcgccgca taatcatcgc
    14401 ccggcgtgat gcctgtaatg ttatagcgcc gatactccgc acgcagcggg ccgttagcat
    14461 caaacaccac acaggaagcg acggtttgtt cgcccatggt atggctgatg tcaaagcact
    14521 ccatccgctt cacttccggc aatttcaaca cgctggcaag cgcggtcagt cgctggtgaa
    14581 cggtagattg ctgcgaaagt ttgctggtta aggccgtcgc cgcattggtg cgcgcgagtt
    14641 tcagataacg cgccctatcg ccgcgaggtt tggtttgaac attaatcttg cgtcccgcca
    14701 gttctgaaag ggaatcggcg agcagcgttt tatcgctaag attaaaatcg agcaggatct
    14761 cacccggtaa ggtgcgcatc tggctgcctt gtaaatagaa ctggcctacg aaggtttcta
    14821 ccacctcgct cagttccgta ccgccaggca ctttcgggaa atagctgcgg ctgccgagca
    14881 ctttgccctg acgaatgaac aatacgtgga cacaagccat gcccgcatcg aacgccacac
    14941 caataacgtc gaggtcgtcg ccggtattgg aaacgaattg tttttcggtg acgcgtcgca
    15001 ccgcctgaat ttggtcgcga atacgtgcag cttcttcaaa ctccagattc tggctggcag
    15061 tttccatacg actaatgagt tgcgtaagca cctgatcatc tttgccagac aaaaacaggc
    15121 gcacatactc aacctgctga gcgtattctt cttcactcac cagtccttca acgcacggtc
    15181 ccagacagcg ccctatctga tattgcagac acggacgcga gcgattgcga taaacactat
    15241 tttcgcactg gcgaatgggg aaaatctttt gcagtagcgc cagtgtttca cgtacggcat
    15301 agccattcgg gaacgggccg aaatattcac ctttggcatg cttcgcacca cgatgcatcg
    15361 ccagacgcgg gtgggtatcg ccactcagga agataaaagg ataagattta tcatcgcgta
    15421 gcaaaacgtt gtaacgcggc tgatagagtt tgatgtagtt gtgttccagc aacagcgctt
    15481 cggtttctgt atgcgtaacc gttacatcaa tttgctggat ctgggcgacc agcgcttcgg
    15541 ttttgcgcga agcgaggttg ctgcggaaat agctggaaag ccgttttttc aggtctttcg
    15601 ctttgccgac atagataacc gtaccaccag catcgtacat gcgataaacg cctggctggc
    15661 aggttacggt ttttaaaaac gcttttgcgt caaactgatc actcactgac ttgataatgt
    15721 ctccgcatta cacagaccat ggcgaattgc caggtgagtc agctcaacat cgccatgaat
    15781 gtttagttta ctgaacatac gatagcggta gctgttcacc gttttcggac tgagattgag
    15841 ctgttctgag atctcattga ccttctggcc cttggtgatc atcagcataa tctgcaattc
    15901 acgttcagac aaactggcaa atgggctttc tgttttttct ggttcgatct ggcttaacgc
    15961 catttgttga gcgatatcag aagcaatgta acgctgccct gaatagacag aacgaatcgc
    16021 actcacgact tcctgcggag ccgcgccttt gctgaggtag cccgcagcac cggcctgcat
    16081 gactttcgct ggtaaagggt tttctgtatg gacggtaagc atgatgattt tgacatcagc
    16141 tgtggaacgc gcgattttac gcgtcgcctc aagaccgcca atgcccggca tactcatgtc
    16201 cattagcacc acgtcaacgg catttgtccg gcaccactta acggcgtctt caccgcacga
    16261 tgcctcaccg acgactttta taccctttat atcttccaga atgcgtcgta tccctgcgcg
    16321 caccagttcg tggtcatcaa caagtagaac gttgatcaaa ggaatatctc cagaaatagg
    16381 gataacgcta ctgatagtta gtcattcgta tattagcggt ttttattgca actttgaaac
    16441 gttaaaaatg gtgggtattc gatttttctc tcgtttttgt aaattcgatt gtccacagtt
    16501 atggtcacgc ccgtcaccaa cggcttccgg gatcgtttca cagtaagccc ggcgatgaaa
    16561 tgtgacaaaa attgacttta ttcagcaaaa atgaaaatca gcccgctgaa tatgtaacat
    16621 taattaaccc gaacaatacg ggcaaaaaac attcatgata aaaatattta ttggtcatta
    16681 tattaacgca tttaatagca ttgttgacgt cacgctcaaa aaacaaccac tgctattttt
    16741 agcaaagctt atggtatact ccgccgcctt aacatttttc accgcaaatt ttcattgcaa
    16801 catgacgagg aaaataaatg agtacgcctg atttttctac tgccgagaat aatcaagaac
    16861 tggcaaatga agtctcctgc ctgaaagcga tgctgacgct gatgctacag gcgatgggac
    16921 aagctgacgc gggccgcgtg atgttaaaga tggaaaaaca gcttgcgctg atcgaagacg
    16981 aaacccaggc tgcagtattt tccaaaacgg ttaagcaaat taaacaagcc taccgtcagt
    17041 aatataaaac cggctgagag cgtgcctttc agccgatttt tgcatctggc acgcagaaag
    17101 aaaagcgatc aaattaagcc agtagcggcc gcgtaacagg ccacctgggt cttatttggt
    17161 gcattgattt ttttctgcat gtttttctga tggaaattga ccgtattctc agagattgac
    17221 aaaatcatcg ctatctctgc tgatgtcttc ccttccgccg tccatttcag aatttctttt
    17281 tcgcgcttgc tgaaattcat ctctggcgtc atcactattt catcatttaa acgcatcaga
    17341 gccatcagac tttcgcgcac cagtaactgc atttttaatt gcagttcatc actaagaatg
    17401 ggtatttcgc gcgtgctgca acgggaaaag gacaaaaagc ccagcgcccg atttggcagc
    17461 attaaatact gagtgacacc gcggcgtaaa ccatgtgcgt gcgcggcttc ccataatggc
    17521 tgtgcttcgc tgaataagtc atcattccac attaaatggc cctgactaaa gttttcaggg
    17581 ttcagcacag gatcaattgc gagaaaattt tttgcctgat aataactaac ccacgactca
    17641 gggtaattgg tgtagaaagc cactttaggt cgcgtgaatg gtaccgggtg gcgaacacat
    17701 aacgaatagt aatcgtactc cagctgctga gcctgaagct ctatttcatg gtagacctct
    17761 tctgcggcct ccatcctctg aaaacgcaac agcatcgtgc gacgccagct gaaaaaatcc
    17821 gtatcctgca tagtaaaccg caacgcccct gagagtgagt atcatttata atgataattc
    17881 aaactatcac ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt
    17941 tgcaagccat cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt
    18001 atcacgcttg aaatggcgtc ccgccccgac aggtgaatcg tcggggctga ttttttctta
    18061 ttattgcagt agaaacttct cgaggaactg gcgggtgcga ggctgctggg gatcagcaaa
    18121 taacgctttt gctgccccct gctcgactat ccgcccctgg tccataaaaa tcgcccggtc
    18181 cgcaacatcc cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc gcttttcctg
    18241 cgccagctgg cggattgtgt tcaggacttc gcccaccagc tctggatcca gcgctgacgt
    18301 tggctcgtca aacaaaatca cctcaggacg cattgccagc gcacgcgcaa tcgctacacg
    18361 ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg ccagcccaac
    18421 tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat
    18481 caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat taaagttctg
    18541 gaagacaaac ccgacgtgct gacgcaactg gcggatcagc gatttttgct gacttaatga
    18601 acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg gttgttccag
    18661 cagatttatg ctgcgtagca acgtggtttt gccggaacca ctcggaccga taattgccac
    18721 cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac cgtgaaattt
    18781 tttcaccagg ttcttaactt caatggcact cattttggtt ctctctcctg gcgattaagt
    18841 tgattctcaa aatggttctg caacgtcgat aatactgtcg ccatgatcca gtagatgagc
    18901 gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga
    18961 cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcctttac caggctgata
    19021 aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc
    19081 atagtctgcc atggcgtcat accaatactg gcagctgctt cccactgacc tttatcaata
    19141 gaagaaatgg cggcgcgcag tgtttcggcg gcataggcgg cagtgttcag cgacagacca
    19201 atcattgctg acggaatggg atctaattcg ataccaaact gcggcaggcc gtaatagatc
    19261 ataaacagtt gtgcgatgag cggtgtacca cgaaaaatag agatataaaa acgcgccagc
    19321 cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc gagcagtaag
    19381 ccaaaaaaca tgccgccgat actgagttgc agcgtatacc cagccccttt cagcaggaac
    19441 ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg agctaaacgt
    19501 ggggatgata ggcgaacagc gcaggcgcac cgccggtatg aataaacaga atcggccctt
    19561 catctttgaa gcgtttctga ctgatacagc caatcagccc cgccatcgct tttccggtat
    19621 acacaggatc aagcagaatg ccttccagcc gcgccagcaa tttcactgct tccatgcctt
    19681 cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtata atttccgctg
    19741 atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact ttcggcaact
    19801 gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc atcaggtgtt
    19861 ccagcccaac agccagtccg gcgtgagttc cggcactgcc cgatgcgact accaccgacg
    19921 aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc acataaccta
    19981 gcgcgccaag agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt
    20041 cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgct tcgcacattt
    20101 caatctgggt attgaacaga tccagcaaca agcgattgcc gttggttaaa tagttttctg
    20161 cggttgtgcc aataggattt tccagcagcg ccacacagtg cagaccgagt ttcgccgcaa
    20221 ctgcggcagt ctgccgcaca tggttagact ggatcacccc ggcagtaatc agcgtatcgg
    20281 caccttcgcg cagagcatct gccgcgagaa attctagctt acgtaattta ttgccgccca
    20341 ttgccatggg ggtgacgtca tcccgtttga tgaaaatttc ccgtcctaga taatcagaaa
    20401 agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt ggaaaacggg
    20461 ttaaattatg cagtggcata acagcctccg atgtatgttg ttgtgatttt cttattatgc
    20521 acgctgaaaa cgcgtaaata aaaaagg
//
LOCUS       DAJTVK010000073        20230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000073 DAJTVK010000000
VERSION     DAJTVK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.073"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            689..1459
                     /locus_tag="OO372_002651"
     CDS             689..1459
                     /locus_tag="OO372_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6342108.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="OO372_002652"
     CDS             1450..1923
                     /locus_tag="OO372_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6342109.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="OO372_002653"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="OO372_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6342110.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="OO372_002654"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="OO372_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6342111.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /locus_tag="OO372_002655"
     CDS             3359..3853
                     /locus_tag="OO372_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6342112.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="OO372_002656"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="OO372_002656"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6342113.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="OO372_002657"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="OO372_002657"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR6342114.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="OO372_002658"
     CDS             7698..8612
                     /locus_tag="OO372_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6342115.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10178
                     /locus_tag="OO372_002659"
                     /pseudo
     CDS             8827..10178
                     /locus_tag="OO372_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="OO372_002660"
                     /pseudo
     CDS             complement(10227..11882)
                     /gene="tsr"
                     /locus_tag="OO372_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="OO372_002661"
     CDS             complement(12001..12447)
                     /gene="hpaR"
                     /locus_tag="OO372_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6342116.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12719..14008
                     /locus_tag="OO372_002662"
     CDS             12719..14008
                     /locus_tag="OO372_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6342117.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14005..15471
                     /gene="hpaE"
                     /locus_tag="OO372_002663"
     CDS             14005..15471
                     /gene="hpaE"
                     /locus_tag="OO372_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6342118.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15473..16324
                     /gene="hpaD"
                     /locus_tag="OO372_002664"
     CDS             15473..16324
                     /gene="hpaD"
                     /locus_tag="OO372_002664"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6342119.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16334..16714
                     /locus_tag="OO372_002665"
     CDS             16334..16714
                     /locus_tag="OO372_002665"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6342120.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16825..17628
                     /gene="hpaH"
                     /locus_tag="OO372_002666"
     CDS             16825..17628
                     /gene="hpaH"
                     /locus_tag="OO372_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6342121.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17639..18427
                     /gene="hpaI"
                     /locus_tag="OO372_002667"
     CDS             17639..18427
                     /gene="hpaI"
                     /locus_tag="OO372_002667"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6342122.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18602..19978
                     /gene="hpaX"
                     /locus_tag="OO372_002668"
     CDS             18602..19978
                     /gene="hpaX"
                     /locus_tag="OO372_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6342123.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19988..>20230
                     /locus_tag="OO372_002669"
     CDS             19988..>20230
                     /locus_tag="OO372_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6342124.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4870 a         5429 c         5168 g         4763 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcaacatc ctgctggggc ttgatccacg
     9961 tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt gccagcttca
    10021 tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc cactcattcc
    10081 gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg tacatcttcc
    10141 tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc cgataacatt
    10201 ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc gctatctgcc
    10261 acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga tgtttcacgc
    10321 tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc ctgctcttcc
    10381 agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg agttacccgg
    10441 tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca
    10501 gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt
    10561 gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca
    10621 atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt acgcacttct
    10681 cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc aaccgccgcg
    10741 ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat aatatcggcg
    10801 attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg
    10861 ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg gcgcgcattt
    10921 tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt ctcttccagc
    10981 gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc gatttcgctg
    11041 gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct
    11101 ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca
    11161 atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt
    11221 ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg
    11281 atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg
    11341 gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg
    11401 tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt
    11461 tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct
    11521 gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc
    11581 gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca
    11641 atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc
    11701 aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca
    11761 gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt
    11821 aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac
    11881 atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat
    11941 gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag
    12001 ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa taaattcctc
    12061 taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata
    12121 ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggttagcga
    12181 gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc
    12241 catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc
    12301 gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt
    12361 caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag
    12421 caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat
    12481 atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt cctgatttta
    12541 tcaaaattga tgatatttta ttaacttact gatatttaat attttattat ctttaaatgt
    12601 aaatagtttg ttaattagat cacatttaca tcacttactt ttgccaaact acgcaatcgg
    12661 gaataaaagc aatcattaac atattaatga ttaagatgat cgaatccgag gagtggtaat
    12721 gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg catggcagga
    12781 agcgttccag caatccccct acaaagcccc gcctaaaact gcggtctggt ttattaaacc
    12841 gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg aaaaggtact
    12901 gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg acgaaagtac gtgaagaaga
    12961 tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc cggaagagag
    13021 cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac
    13081 cgtggctctc agcaatgtcg ataatctgac catctatacc gagatcaacg ggcgtcctgc
    13141 cgatcactgg aataccgccg atttacaacg taacgccgcg cagttgctga gcgccctgag
    13201 cgaatttgcc acgctgaatc caggcgacgc cattctgctc ggcacgccac aggcgcgcgt
    13261 ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa ggtttcccgc cgctggaaaa
    13321 tccggtagtg gacgaacgtg aagtgaccac gcacaagagc ttcccaacgc agccacaccc
    13381 gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg aactggaatt
    13441 taagccaccg gaagaaccgc tggtgttcct gaaagcgcca aataccctca ctggcgataa
    13501 ccagacctcc gtgcgtccaa acaatattga atacatgcac tatgaagcgg agctggtggt
    13561 agttattggc aaacaggcgc gtaacgtcag cgaagccgat gccatggatt atgtcgcggg
    13621 ctacaccgtg tgtaacgact acgccattcg tgactatctg gaaaactact accgccctaa
    13681 cctgcgggta aaaagccgcg acggactgac gccgatgctt tcaaccatcg tgccgaaaga
    13741 ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg agttacgcca
    13801 gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct acttaagtga
    13861 atttatgacc ctgaatccgg gcgacatgat cgccaccggc acacccaaag gcttatctga
    13921 cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg
    13981 aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat
    14041 gttgcaggta acgactactt ccagaccacc aatccggcaa cgggtgaagt gctggcggat
    14101 gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa agaggcgttc
    14161 ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat
    14221 ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa ccgcggacac cggcctgccg
    14281 atccatcaga ccaaaaatgt gttgatccca cgcgcttccc acaactttga atttttcgcg
    14341 gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat gctcaactac
    14401 acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc cgtggaacgt accgtttatg
    14461 accgccacat ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt actgaaaatg
    14521 tcggaactct ccccgctgac cgctgacagc ctgggtgagc tggcgctgga agccgatatt
    14581 ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga tgcgctggtt
    14641 cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg gcgcaacatc
    14701 atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc gccggtgctg
    14761 atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac catcttctcg
    14821 atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcaaag catctacccg
    14881 gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat
    14941 ccgaataccc aggttggcgc gcttattagc cagcagcact gggaaaaagt ctccggctat
    15001 atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg gcggcccgga taaaccgtcc
    15061 gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caaccgtgct ggcagacgtt
    15121 gataaccgta tgcgagtcgc ccaggaagag attttcgggc cggtcgcctg cctgctgccg
    15181 tttaaagacg aagccgaagg cttacgtctg gcaaacgacg tggagtacgg cctcgcgtcg
    15241 tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat tgaagctggc
    15301 atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg cggcgtaaaa
    15361 gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag
    15421 aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg atatgggtaa
    15481 gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatcactctg aactgccagg
    15541 gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca gcaagcgttg
    15601 ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg tcaacagtgc
    15661 ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg agctgccgca
    15721 ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc agcttattgc
    15781 cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca gcctgaaact
    15841 ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact tcaaagtggt
    15901 ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc tgggcgaagc
    15961 gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt
    16021 atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga
    16081 gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc agttcaaaga
    16141 gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga
    16201 cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat
    16261 caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgcttcccgc
    16321 gtaaggacgt tttatgccgc actttatcgt taaatgcagt gacaacatcc gcgaagaagc
    16381 cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg gtatttttcc
    16441 gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg ccgacgggca
    16501 gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca gcctggaaag
    16561 ccgccagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg ccgccctgat
    16621 ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga cgctgaattt
    16681 taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg tggcgctgcg
    16741 cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc gcagccgcat
    16801 ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct gatcgcccag
    16861 cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct ggattacccg
    16921 gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat gggttcgcct gaaaatcgcc
    16981 gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct cgaaagcgat gcaggcaagc
    17041 tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg acatgttctt ccacgatggc
    17101 agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct ggctttcgtg
    17161 ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa cgccacggac
    17221 tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga tccggaaacc
    17281 cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc cggggtgatc
    17341 ctcggcggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc cgccctgatg
    17401 tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa tcatccggca
    17461 aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact ggaagccggg
    17521 caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg cgacaccttc
    17581 cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg agagaacgat
    17641 ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat tatggctggg
    17701 gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact ggttgttgat
    17761 cgacggtgag cacacgccga ataacgtgca aaccgtgctc acccagctac aggcgattgc
    17821 gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc aaatcaaaca
    17881 actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg ctgacgaagc
    17941 ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt attcgcggtg tgggcagtgc
    18001 gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag ccaacgatca
    18061 aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac cgcagattct
    18121 ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg ccgatatggg
    18181 ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg cgatcgtgca
    18241 gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc aatgagcaac tggcaaaacg
    18301 ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc tgctcgcccg
    18361 cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga agcccggcgt
    18421 gtattaatgc cggatgcgct gcgcttatcc ggcctacact cgcaccaaac cgtaggccgg
    18481 ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc ggataaggcg
    18541 ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta gaggaagaaa
    18601 aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc agcataaagc
    18661 gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga gcgtctttct
    18721 gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg ccggactcac
    18781 gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca ccctgttcta
    18841 tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg tcggtgcgcg
    18901 gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca ccatgtttgc
    18961 cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg aagccggctt
    19021 tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc gcgcccgtgc
    19081 caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga tcgtttccgg
    19141 ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc tgtttttgct
    19201 ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg atgactcacc
    19261 ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga tgatggataa
    19321 cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca tgcaacaacg
    19381 cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg cgtatttctg
    19441 cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc aaagttttaa
    19501 tcagggcagc agtaatatca ccatcggcat gctggccgcc gtaccgcaga tttgtaccat
    19561 tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa ggcatcacac
    19621 cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa ctgatcacaa
    19681 catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg caatggcgat
    19741 tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg gtattgcggt
    19801 gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg gctggttgaa
    19861 agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc tggtgattgg
    19921 tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga ccctgtaagg
    19981 aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt acgatgaaag
    20041 cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat tttttggccg
    20101 ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca atagcggaca
    20161 gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt ttgtcctgac
    20221 gccgccgtca
//
LOCUS       DAJTVK010000074        20147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000074 DAJTVK010000000
VERSION     DAJTVK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.074"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1277)
                     /locus_tag="OO372_002670"
     CDS             complement(<1..1277)
                     /locus_tag="OO372_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342125.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
     gene            complement(1470..1539)
                     /locus_tag="OO372_002671"
     tRNA            complement(1470..1539)
                     /locus_tag="OO372_002671"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Gly,seq:ccc)
     gene            complement(1618..2373)
                     /locus_tag="OO372_002672"
     CDS             complement(1618..2373)
                     /locus_tag="OO372_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6342126.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2788..5085
                     /gene="xdhA"
                     /locus_tag="OO372_002673"
     CDS             2788..5085
                     /gene="xdhA"
                     /locus_tag="OO372_002673"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR6342127.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5096..5974
                     /gene="xdhB"
                     /locus_tag="OO372_002674"
     CDS             5096..5974
                     /gene="xdhB"
                     /locus_tag="OO372_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6342128.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5971..6450
                     /gene="xdhC"
                     /locus_tag="OO372_002675"
     CDS             5971..6450
                     /gene="xdhC"
                     /locus_tag="OO372_002675"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR6342129.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6490..8268)
                     /locus_tag="OO372_002676"
     CDS             complement(6490..8268)
                     /locus_tag="OO372_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR6342130.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            8747..9934
                     /gene="ygeW"
                     /locus_tag="OO372_002677"
     CDS             8747..9934
                     /gene="ygeW"
                     /locus_tag="OO372_002677"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR6342131.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9992..11188
                     /gene="dpaL"
                     /locus_tag="OO372_002678"
     CDS             9992..11188
                     /gene="dpaL"
                     /locus_tag="OO372_002678"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR6342132.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            11246..12457
                     /locus_tag="OO372_002679"
     CDS             11246..12457
                     /locus_tag="OO372_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR6342133.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            12510..13894
                     /gene="hyuA"
                     /locus_tag="OO372_002680"
                     /pseudo
     CDS             12510..13894
                     /gene="hyuA"
                     /locus_tag="OO372_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13942..14874
                     /gene="arcC"
                     /locus_tag="OO372_002681"
     CDS             13942..14874
                     /gene="arcC"
                     /locus_tag="OO372_002681"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6342134.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14915..16540)
                     /locus_tag="OO372_002682"
     CDS             complement(14915..16540)
                     /locus_tag="OO372_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR6342135.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(16588..17358)
                     /gene="yqeC"
                     /locus_tag="OO372_002683"
     CDS             complement(16588..17358)
                     /gene="yqeC"
                     /locus_tag="OO372_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR6342136.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            17461..18039
                     /gene="mocA"
                     /locus_tag="OO372_002684"
     CDS             17461..18039
                     /gene="mocA"
                     /locus_tag="OO372_002684"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR6342137.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            18361..>20147
                     /gene="ygfK"
                     /locus_tag="OO372_002685"
     CDS             18361..>20147
                     /gene="ygfK"
                     /locus_tag="OO372_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCR6342138.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         5225 a         5180 c         4764 g         4978 t
ORIGIN      
        1 cttttcgcat gtgaattttg atcggtatcg gttttaatat cattgaacca aaaatttgcc
       61 gactgataat tatccttctc gtatcttttt ataccatacg gacctgctag ttttttaact
      121 atttttagaa cctgctcttt tcttctggtg tttatctttg caagatttgc aggataaata
      181 acattaagta aagccgcatc ggcttctctg taatgtttat ctttttccag ataacctggg
      241 gattctcccc ctaaatcaag ctgtaacgtt attctttcat aacctttatc tatgaggtgg
      301 ttcaacctcg ttgttgaaag aggttcatcc agttcattgg ctttagcttc gcgtagtaaa
      361 tcagagacaa aaacagagtc tttcttcgag agtaagttcg acaaacgttc cagtccagag
      421 gtcaccagtg caacggaaga tgtgtttaat cgggcatctt cttcccatgc tccggaatct
      481 tccataacat agaaattggc tttatccaga taagcaatca gcagcgcaac cgattttaat
      541 cggtcacctt tttgccaatc ttcagcgtta attgtaccag tgtcgattgc ttgtataagg
      601 agatcaagat aaagacctaa cgcatcattt tgtttatggt tccacagctg cggcttgcct
      661 tcttcctgca catctgccat cacgggggag ttgctgtcaa atcggatatg cacagcattc
      721 atttgcccgg gaactccatc cagtcgtttg gggttgctaa tgacgtcctg catccgctta
      781 atctggtcga gcgtagacat ataatcccac agggtgagca gaaccttttt tgcggctaca
      841 ctatttccct gatctgatac taatgccata tagccccata aactatccct cagccagaca
      901 gcatcatagt gggtttctgc cgctgttttg tcatcttcga aagtagaagc aagaataaat
      961 gttccccagg gtttctccgt caacgttaac tttcgtgtgt tttctgttaa tgttaacaac
     1021 ccttgaattt cgtctttggt atagtggctc tttatttcat tttttaattt ggctgagtga
     1081 atcagaagac gataattagg gtacttatct ggatttaacg ataaataact tttatctgtt
     1141 gctgcccccg tggaattaag aatgttaaat acagattcac tcgtcacttc tgcccctgtc
     1201 gcctggcatg tgccgcacga aaatatacca gcgagcagaa gagttaatgc agatttccct
     1261 ttcataaaat attccatcaa tcgtttgtta aaagcgtaaa taaccactta tttaacaact
     1321 tgaaaataag gtgttttacc tgggttgtta caaaaggatt gcattgcgta aacgcttttt
     1381 atttacaaca aaatggggaa atattacggc gattgcgaaa agtctgaagc gattgaatta
     1441 agataatata gaaaatcaga tacagcaaaa gcggcgaagg gaatcgaacc ctcgtataga
     1501 gcttgggaag ctctcgttct accattgaac tacgcccgct tcgagatgcg taaggcatta
     1561 taaaccttac gctctcctta gcaagtgccg cgctgctgac tgctgattaa ttcgccatca
     1621 gcattttggc ttgctgccct gcggcggcaa gtaacgtagc ggatcaattg ccgttgcacg
     1681 gtaacgaatc tggaaatgca ggcgaacaga tgccgcatcc gtgctcccca tagtggcgat
     1741 tttttgccca gccttcacgc tttgcccatt atttaccagc atcgtgtcat tatgggcgta
     1801 agccgtaatg taatcttcac tgtgtttaat catgatgaga ttaccgtagc cacgcagctg
     1861 gttgcccaca tacaccacct ttcctgcacc cgcggcgtaa ataggtgtac cccgtggagc
     1921 tgagatatca atccctttat tgccgccatc tgctgtcgaa tacggcatga taactttccc
     1981 tgtcgttggc cataaccaac aacgttgccc tactggcggc caggatgatt tcggtaccgc
     2041 tgatgacggt gtaaccgatg cggttttggt cgttgatttg gcggttgatt tacgtgtact
     2101 actgctactt ttcgccccac ccagttttag tttctgacca acttcaatgg tgtaaggggg
     2161 ggaaatgccg ttcagtcgcg ccagttcttt tacgctggtt cccgtggtgc gcgaaatacg
     2221 atatagcgta tccccccgtt tcacggtgta aacggagccg gaatacgttc ctgtatcgga
     2281 tgatttgcta cccgaacagc ccgccaaaag cagtccaacc gataacaaca tcacgatacc
     2341 cagagatttt ttattcaggc gtcccgcact caaaatcttt cctcgctaaa aaaacaagac
     2401 gccttatcat agcagccagc ctcttgatac ccaatctctt gcttctggaa aaacgtggta
     2461 atagagaaag aggttaattt catttggcaa agggaagtat catcagcaaa atattcgctg
     2521 tagaggtatc gaacttcctg actttttacc ctatttccca tcctgatgcg taataatccc
     2581 aatttatcac attgatttta tcgccactca tattgatacg tatcactgcc cctattcaga
     2641 gtcctgtttc gtatcatatc tgtgtttttt taacgtcatc acacttccta cctcccctaa
     2701 cgcttatcgt cgtttctggc gtaaatcttg cctgcttaga ctaaatcttt gccataagaa
     2761 ccaaatgttc aaatcgaggg gatttctatg gaagcgcggg aagcaaccgc tacgggtgaa
     2821 tcatgcatgc gcgtcgatgc cattgctaag gtcaccgggc gggcacgata tactgacgat
     2881 tatgttatgg cgggcatgtg ttatgcgaaa tatgtacgta gccctatcgc acatggttat
     2941 gccgtaagta ttaatgatga acaagccaga agtttgccgg gcgtactggc gatttttacc
     3001 tgggaagatg tgcctgatat tccattcgct acagctgggc atgcctggac acttgacgaa
     3061 aacaagcgcg ataccgccga tcgcgcactg ctaactcgcc atgttcgtca tcatggtgac
     3121 gccgttgcca tcgtcgtggc ccgcgatgaa ctcacggcag aaaaagcggc gcaattggtc
     3181 agcattgagt ggcaagaatt acccgttatc accacgccag aagcggcgct ggcagaagac
     3241 gctgcaccaa tccataacgg tggcaattta ctgaaacaaa gcacgatgtc gacgggtaat
     3301 gtccaacaaa caatcgatgc cgccgactac caggtacagg ggcactatca gacccccgtt
     3361 attcaacatt gtcacatgga aagcgtaaca tcgctggcgt ggatggagga tgactcgcga
     3421 attaccatcg tttccagcac ccagatcccg cacattgttc gccgcgtggt tggtcaggcg
     3481 ctggatattc cctggtcatg cgtacgagtc atcaaaccgt ttgtcggtgg cggttttggt
     3541 aataaacagg atgtactgga agagccaatg gcagcattcc tgaccagcaa gcttggcggc
     3601 attccggtga aagtttccct tagccgtgaa gagtgtttcc tcgcaacccg tacccgccac
     3661 gcttttacca ttgacgggca aatgggcgtg aaccgcgacg gaacattgaa aggttatagt
     3721 ctggatgttc tgtctaacac cggcgcttat gcatctcacg ggcactccat cgcttctgcg
     3781 gggaggaata aagtcgctta cctttatcct cgttgtgcct acgcttacag ttcaaagacc
     3841 tgctatacca acctcccctc ggctggtgcg atgcgtggtt atggcgcgcc acaagtcgta
     3901 tttgccgttg agtctatgct tgatgacgcc gcgacagcgt taggtattga tcctgttgaa
     3961 attcgtttac gcaacgccgc ccgcgaagga gatgctaatc cgctcacggg caaacgtatt
     4021 tacagcgcag ggttgccgga gtgtcttgaa aaaggccgga aaatctttga atgggaaaaa
     4081 cgccgtgcag aatgccagaa ccagcaaggc aatttgcgcc gcggcgttgg cgtcgcctgt
     4141 tttagctata cctctaacac ctggcctgtc ggcgtagaaa tagcaggcgc gcgccttctg
     4201 atgaatcagg atggaaccat caacgtgcaa agcggcgcga cggaaatcgg tcagggtgcc
     4261 gacaccgtct tctcgcaaat ggtggcagaa accgtggggg ttccggtcag cgacgttcgc
     4321 gttatttcaa ctcaagatac cgacgttacg ccgttcgctc ccggcgcatt tgcctcacgc
     4381 cagagctatg ttgccgcgcc tgcgctgcgc agtgcggcac tattattaaa agagaaaatc
     4441 atcgctcacg ccgcagtcat gctacatcag tcagcgatga atctgaccct gataaaaggc
     4501 catatcgtgc tggttgaacg accggaagag ccgttaatgt cgttaaaaga tttggcgatg
     4561 gacgctttct accaccctga acgcggcggg cagctctctg ctgaaagctc catcaaaacc
     4621 accactaacc catcagcgtt tggctgtacc tttgttgatc tgacggtcga tattgcgctg
     4681 tgcaaagtca ccatcaaccg catcctcaac gttcatgatt cagggcatat tcttaatcca
     4741 ctgctggcag aaggtcaggt acacggcgga atgggaatgg gcattggctg ggcgctattt
     4801 gaagagatga tcatcgatgc taaaagcggc gtggtccgta accccaatct gctggattac
     4861 aaaatgccga ccatgccgga tctgccacaa ctggaaagcg cgttcgtcga aatcaatgag
     4921 ccgcaatccg catacggaca taagtcactg ggtgagccac caataattcc tgttgccgct
     4981 gctattcgta acgcggtgaa gatggctacc ggtgttgcaa tcaatacact gccgctgacg
     5041 ccaaaacggt tatatgaaga gttccatctg gcaggattga tttgaggata acatcatgtt
     5101 tgattttgct tcttaccatc gcgcagcaac ccttgccgat gccatcaacc tgctggctga
     5161 caacccgcag gccaaactgc tcgccgatgg cactgacgta ctgattcagc tccaccatca
     5221 caatgaccgt tatcgccata ttgttgatat tcataatctg gcggagctgc ggggaattac
     5281 gctggcggaa gatggctcgc tacgtatcgg ctctgcaacg acatttaccc agctaataga
     5341 agctcctata actcaacgtc atctcccggc gttatgtgct gcggccacgt ccattgctgg
     5401 accgcagatc cgtaacgtcg ctacctacgg tggaaatatt tgcaacggtg ccaccagcgc
     5461 agattctgcc acgccaacgc taatttatga cgcgaaactg gagatccact ccccgcgcgg
     5521 tgttcgtttc gtcccgatta atggctttca caccgggccg ggcaaagtgt ctcttgagca
     5581 tgacgaaatc ctcgtcgcct ttcattttcc gccacagccg aaagaacacg cgggcagcgc
     5641 gcattttaaa tatgccatgc gcgacgcaat ggatatttca acgattggca gcgccgcaca
     5701 ttgccgactg gataacggca atttcagcga attacgcctg gcatttggtg ttgccgcgcc
     5761 aacgccgatt cgctgccaac atgccgaaca gactgcacaa aatgcgccat taaacctgca
     5821 aacgctggaa gctatcagcg aatctgtcct gcaagatgtc gccccgcgtt cttcatggcg
     5881 ggccagtaaa gagtttcgtc tgcatctcat ccagacgatg accaaaaaag tgattagcga
     5941 agccgtcgcc gcggcggggg gaaaattgca atgaatcaca gcgaaacaat taccatcgaa
     6001 tgcaccatta acgggatgcc ttttcagctt cacgccgcac caggcacgcc gctctcggaa
     6061 ttactccgcg aacaaggact gctaagtgtc aaacaagggt gctgcgtggg tgaatgtggt
     6121 gcctgtacgg tgttggtcga cggcacagca atagacagtt gcttatacct tgccgcctgg
     6181 gctgaaggaa aggagatccg cacgctggaa ggtgaagcga aaggcggaaa actttctcat
     6241 gttcagcagg cttatgcgaa atccggcgca gtgcagtgcg ggttttgtac gcctggcctg
     6301 attatggcta ccacggcaat gctggcgaaa ccacgcgaga agccattaac cattacggaa
     6361 attcgtcgcg gactggcggg aaatctttgt cgctgcacgg ggtatcagat gattgtaaat
     6421 acagttctgg attgcgagaa aacgaagtaa aaggatatcc ggcctgaatt caggccggat
     6481 tcactgaggt tatgtgttta acaactcata tttcttaatc ttgcgataga gcgtagcaat
     6541 gccgatgccc agttcatcag caacttgctt cttgctgtta tgacgtgaaa gcgcctcgcg
     6601 gatcatttgc ttttccatct cctccagcgc cgtgccgccc gcatcatcga gtgacaggtg
     6661 cgcctcactg acctctgtta catcactttg ctccgttgtg ccattattca gcagatttgg
     6721 cggcaatagc gtgctgtcga taacttcacc tgaaggaacc acgttaacca gatattccat
     6781 caaattgctt aactcgcgca ggtttccggg ccaacgatgc ttacgcaata tttcgacgac
     6841 atcgggagca atgccaggat aaaccgatcc cagacgacgg gtatgcagat gtaaaaagta
     6901 atgcaccaat agttcaatat cttcctgacg ttcacgcagc ggtggcagag ttatcgggat
     6961 aacattaagt cggtagaaga gatcttcgcg gaatttacct tcggcaatga actgggccaa
     7021 attctgatta gttgcagaaa tgatgcgaat gtcgacttgt attgggctac tggcaccaat
     7081 cggcagaatt tcacgcgcct caatagcgcg cagtaattta gcctgcaaca ttaatggcat
     7141 atcacctatt tcatcgagaa acagcgtgcc cgtattcgcc gcctgaatca accctgtttt
     7201 accgttggca gaagcaccag taaatgcgcc tttaacataa ccgaacagtt cgctttccag
     7261 aagctgctcc ggaatcgcgg cacagttgat agcaataaag ggtttattcc gtcttccgct
     7321 caacttatgg attgctcggg cgacgacttc tttacccgtg ccgctttcac caaccaccat
     7381 aacgctggat gggctgggtg caatacggct aatgagtcgt tttaattgcc gcataacacg
     7441 gcactcgcca accaattgtt caatatgtgg ttcatcaggt gcatttgcta cagaaaaact
     7501 ggtatgcgat tggtgaaatg ccattaaaaa taattgtcgg ccctgaatgt tatgcaattg
     7561 accaatgatt aattcacttt tatcgtccca cgaaacaata tgctgcatat gtccatgagt
     7621 aaaattactc tcaaatgtta atggtctgaa acggataggt ttcccaataa tattattttg
     7681 tacaacacca agtgttttta aggcagtctg attaacaaac tgaacccgac tttcatcatc
     7741 aacaactaat acgccctgat ccatattatc gatcatggtc gcgaatattt tactgatgtt
     7801 atctcctggc ccctgatcct ccagaagttt cgaaacaaaa atggtggata tatggcgaac
     7861 ataatcagaa aattcgcgta aattatcact gatatgctct tgttgctcgt gggtaacggc
     7921 aatcaaactt atcaccccaa cacaacgatc ctgtaaaatg acaggcgtac ccagaaatgc
     7981 tttttcgcgg caattttctt tactatcgca accttcgcaa aggggatcga agcgagactg
     8041 tgtcacaact ttttcagttt tcgtttccag gacgtggcgg agcaggcgtg agttgccgct
     8101 caactggcga ccaagaaact tcccatacgc gcccgttccg gcgacgcgac acaagttttc
     8161 atcaacgatc tcaacctcaa actgcaaaac gctggcaagc attctggcaa aacgctgaat
     8221 tgtcggttga atttgcatca atactgactg cgtagtagca agctccatag ctttaccttc
     8281 cagacttact taaaagtcga tcattgaaga cgttgatggt tcacagatca tgatgatatt
     8341 aactcaggcg aaattggctt tgataaaaac ataagatttt tatcattttc taatgcaatt
     8401 atggaagaga tatcacattt ctatatcaat atgagaatta cggcggtgag tttatcaaac
     8461 tgaagagaga tagcctgccc ctttatctta tttctgatac ttagcagaaa ataaataacg
     8521 cgataaaaaa agccaaacgt tttcgtattt tacaaacaac cagaagctgg catcaatttg
     8581 tgaacaaccc cacacattat ccgtcaaatt agtcttttgc agccgcgcgg ataattctgg
     8641 cacacttatt gttagtccca ggtatagcta tgaaaacacc aatcactttg gcaagtcaca
     8701 gtgaaataaa ccactttgcc tgtcattcca ctaccgggac tgtatgatga aaactgttaa
     8761 tgagctgatt aaggatatca attcgctgac ctctcacctt cacgagaaag attttttgtt
     8821 aacgtgggaa cagacgccag atgaactgaa acaagtactg gacgttgccg cagcattaaa
     8881 agcactgcgt gctgaaaaca tctcaaccaa agtctttaat agtggattag gtatttccgt
     8941 attccgcgac aactccaccc gtacccgctt ctcttatgct tccgcgctta acctgctcgg
     9001 ccttgcacaa caagatctcg acgaaggcaa atcacaaatc gctcacggcg aaaccgtgcg
     9061 tgaaaccgcc aatatgatct ccttctgcgc cgacgctatt ggtattcgcg acgatatgta
     9121 tctgggcgca ggcaacgcct atatgcgtga agttggcgct gcacttgatg acggttacaa
     9181 gcagggtgta ctgccacagc gtccggcttt agtgaacctg caatgcgata ttgaccaccc
     9241 gactcagtca atggctgacc tcgcgtggtt acgtgaacac tttggttcac tggaaaacct
     9301 gaaaggtaaa aaaatcgcca tgacctgggc ctactctcca agctatggca aaccgctctc
     9361 tgtaccacaa ggcatcatcg gtctgatgac tcgcttcggt atggatgtca ccttggccca
     9421 tccggaaggc tacgacctga tcccggatgt ggttgaagtg gcgaaaaaca atgctaaagc
     9481 ctccggtggt agcttccgtc aggtcaccag catggaagaa gccttcaaag acgcagacat
     9541 cgtttatccg aagtcatggg caccttacaa agtgatggaa gagcgtactg aattgctgcg
     9601 tgcgaacgac cacgaaggct taaaagcact ggaaaaacag tgtctggcac agaacgcgca
     9661 acacaaagac tggcattgta ctgaagagat gatggaactg acccgtgatg gcgaagccct
     9721 gtacatgcac tgcctgccag ctgatatcag cggcgtatcc tgcaaagaag gtgaagtgac
     9781 tgaaggcgta ttcgaaaaat accgtatcgc tacctacaaa gaagccagct ggaagcctta
     9841 tatcatcgcc gcgatgatcc tgtcccgtaa atacgccaaa ccaggtgcac tgctcgagca
     9901 actgctgaaa gaagcgcaag aacgcgtgaa ataacccctc gggccgatct gctggtcggc
     9961 cctctatttc cagaggccaa aaggatagga tatgtccgtt ttctcattga agattgatat
    10021 cgccgataac aaatttttca acggcgaaac atcaccgctc ttttcgcaaa gccaggccaa
    10081 actggcgcgc cagttccacc agaaaatagc tggttatcgc ccaacaccgc tttgtgcgct
    10141 ggacgatctc gcaaaccttt ttggtgtgaa gaaaattctc gtcaaagacg aatcaaaacg
    10201 attcggtctg aacgccttca aaatgcttgg cggtgcgtac gccatcgctc aattattgtg
    10261 tgaaaaatat catcttgata tcgaaacgct gtcatttgag cacctgaaaa atgccatcgg
    10321 cgaaaaaatg actttcgcga ccaccaccga cggcaaccac gggcgcggtg tggcgtgggc
    10381 agcacagcaa ctcggacaga atgcggtgat ttacatgccg aaaggttctg ctcaggaacg
    10441 cgttgacgcc attctgaacc tcggtgccga gtgcatcgtc acggatatga actatgacga
    10501 taccgttcgc ctgaccatgc aacacgcgca gcagcacggc tgggaagtgg tacaggacac
    10561 ggcatgggaa ggttacacca aaatcccaac ctggatcatg caaggctacg caaccctggc
    10621 agatgaagcc gtcgagcaaa tgcgtgaaat gggcgtaacc ccgacgcacg ttctgctgca
    10681 agccggtgtc ggagcaatgg ccggtggtgt gctgggttat ctggtcgacg tctacagccc
    10741 gcaaaatctg cacagcatta ttgttgaacc tgacaaagct gactgtattt atcgctccgg
    10801 cgtcaaaggc gacatcgtca acgttggcgg tgatatggcc accatcatgg cgggcctggc
    10861 ctgtggcgaa cctaacccgc tgggctggga aatcctacgt aactgcgcca cccaattcat
    10921 ctcctgccag gacagcgttg ccgcattagg tatgcgcgtg ctgggtaatc cgtacggcaa
    10981 cgacccgcgc atcatctccg gtgaatccgg cgctgtcggt ttgggcgttc tcgcagcggt
    11041 tcattatcac ccgcaacatc aaagcctgat ggaaaaactg gcgctggaca aagatgccgt
    11101 agtgctggtt atcagcactg aaggcgacac cgacgtgaag cactaccgcg aagttgtctg
    11161 ggaaggcaaa cacgctgtag caccttaatc accctattgg aactactccc tgcaaaacgg
    11221 ggagtaaaaa atctggagaa aaataatggc taagaatatt ccattcaaac tgattcttga
    11281 aaaagcaaaa gattaccagg cggacatgac tcgcttcctg cgcgacatgg ttgctattcc
    11341 cagtgaaagc tgcgacgaaa aacgcgtagt acatcgtatt aaagaagaga tggaaaaagt
    11401 cggcttcgat aaagttgaaa tcgacccgat gggcaacgtt ctcggttata tcggccacgg
    11461 cccgcgtctg gtggcaatgg acgctcatat cgataccgtc ggcattggca acatcaaaaa
    11521 ctgggacttc gatccgtacg aaggcatgga aactgatgag ctaatcggtg gtcgcggtac
    11581 ttccgaccag gaaggcggca tggcatctat ggtttatgcc ggtaaaatca ttaaagacct
    11641 cggtctggaa gatgaatata ccctgctggt taccggtact gtgcaggaag aagactgcga
    11701 cggtctgtgc tggcagtaca ttattgaaca atccggcatt cgcccggaat ttgtggtcag
    11761 taccgaacca accgactgcc agatataccg tggtcagcgc ggtcgtatgg aaattcgtat
    11821 tgatgttcag ggtgttagct gccacggttc tgcgccagaa cgcggtgaca acgccatttt
    11881 caaaatgggt ccgattcttg gcgaattaca agaactctcc caacgtctgg gttatgacga
    11941 attcctcggc aaaggcaccc tcaccgtttc tgaaatcttc ttcacatccc caagccgttg
    12001 cgctgtagca gatagctgcg ccgtctctat tgaccgccgt ctgacctggg gcgaaacctg
    12061 ggaaggcgcg ctggacgaaa tccgcgccct gcctgcagta cagaaagcta acgcggttgt
    12121 ttctatgtac aactacgacc gtccgtcctg gactggcctg gtttacccaa ccgaatgcta
    12181 cttcccgacc tggaaagtgg aagaagatca cttcaccgtt aaagcactgg tgaatgccta
    12241 cgaagggctg tttggcaaag cgccggttgt tgataagtgg accttctcaa ctaacggcgt
    12301 atctatcatg ggccgtcacg gcattccggt gatcggcttt ggcccgggta aagaacctga
    12361 agcgcatgca cctaacgaaa aaacctggaa atctcacctg gtgacctgtg ccgcgatgta
    12421 cgctgcaatc ccgttaagct ggctggcaac agagtaatta ctcttcactt attcccctcc
    12481 ggttcgccgg agggtttttg gagtttgcta tgcgcgtatt gatcaaaaac ggcactgtcg
    12541 ttaacgcaga tggacaagcc aaacaggatt tgctgattga aagcgggatt gttcgccagt
    12601 tgggcaacaa tatttcgccg cagctcccgt atgaagaaat tgatgccact ggctgttacg
    12661 ttttccctgg cggcgtggat gtccatacgc atttcaatat tgatgtcggc atcgcgcgca
    12721 gttgtgatga ttttttaccg gtacccgcgc agctgcgtgt ggcggtacaa caaccattat
    12781 tgaccatatg ggatttggcc caaacggctg tcggttacgc catcaactgg aggtttatcg
    12841 tggttatgcc gcccataaag cggtcatcga ttacagcttt cacggtgtga tccagcacat
    12901 taatcacgca atcctcgacg aaatcccgat gatggtcgag gaaggactga gcagttttaa
    12961 actctattta acctatcaat acaaactcaa cgatgacgag gttttgcagg cattacgccg
    13021 cttgcatgaa tccggcgcgc tgaccaccgt gcacccggaa aatgatgcgg ctatcgccag
    13081 caagcgggcg gaatttatcg ccgcagggtt aaccgcgccg cgctatcatg ccttgagtcg
    13141 ccctctggaa tgcgaagcgg aagccatcgc ccgcatgatt aacctggcac aaattgccgg
    13201 taacgccccg ctctatatcg tgcacctgtc taacggctta ggtctggatt atctgcgtct
    13261 tgcccgtgcg aatcaccagc cagtctgggt tgaaacctgc ccacaatatc tcctgttgga
    13321 cgaacgcagt tacgatacag aagatggcat gaagttcatt cttagcccac cgctgcgtaa
    13381 cgtacgcgag caggacaaac tgtggtgtgg catcagcgat ggtgcgattg acgtggtggc
    13441 gaccgatcac tgcaccttct cgatggctca acgcctgcaa atttctaaag gcgatttcag
    13501 tcgctgccca aatggcttac ccggtgtgga aaaccgcatg cagttactgt tttccagtgg
    13561 cgtgatgacg ggacgtataa caccggaacg ctttgttgaa ttaaccagcg caatgcccgc
    13621 caggctgttt ggcctgtggc cgcaaaaagg attattagcg cccggttccg atggcgacgt
    13681 ggtgattatc gacccacgtc agagccaaca aattcagcat cgccatctcc acgacaacgc
    13741 cgactactcg ccatgggagg gttttacctg tcagggcgcg attgtcagaa ccttatcccg
    13801 tggtgaaacg attttctgtg acggcacctt tacaggcaaa gccgggcgag gtcgtttcct
    13861 gcgacgcaaa ccgtttgtcc ctcccgtgct ctaaccactg tcagggatct atacacaatc
    13921 aagaatgatg agtgaggaaa aatgagtaag aaaattgttc tcgccctggg cgggaatgcg
    13981 ctgggcgatg acctggccgg acaaatgaaa gcggtaaaaa ttacttctca ggcaattgtt
    14041 gatttaattg cccaaggaca tgaagttatc gtcactcatg gtaacgggcc gcaggtgggc
    14101 atgattaacc aagcctttga agctgccgca aaaactgaag cacactcgcc aatgctgccg
    14161 atgtctgtct gtgttgcctt aagccagggt tatattggtt acgatctaca aaacgcgtta
    14221 agagaagaac tgctttctcg gggcattaat aaacctgtag caacgctggt tactcaggtc
    14281 gaagtcgacg ctaacgatcc ggcattcctc aacccgacca agccgatcgg ttcgttcttt
    14341 accgagcaag aagcagagca actgacaaaa cagggttaca ctctgaaaga agatgctggt
    14401 cgcggctatc gccgtgttgt tgcctcgcca aaaccggttg atatcattga aaaagaaacg
    14461 gttaaagctc tggtagatgc aggccaggtg gtgatcgccg ttggcggtgg tggtatccct
    14521 gttattcgtg aaggtaacca tctgcgtggt gccagcgcgg ttatcgataa agactgggcc
    14581 agcgcccgtt tagcagaaat gattgatgcc gatatgctga tcattctgac tgcggtagaa
    14641 aaagtggcca ttaacttcgg taaagagaat gaacagtggc tcgatcgcct gtcattaagt
    14701 gatgctgaac gctttatcga agaaggtcat tttgcgaaag gttctatgct gcctaaagtg
    14761 gaagctgccg cctcgtttgc tcgctctcgc gcaggtcgtg aagcactgat taccgtattg
    14821 agcaaagcga aagaagggat tgaaggcaaa accggaacgg tgatttgtca gtaattttaa
    14881 ggccgggtgg gtaataccat ccggccattt tcatttaagc cacttccagc acttcttttg
    14941 tcgctttcac gccctgatgc atcaacatca ttaacgcctc aagtacgccg ccgccaatcg
    15001 cccgggcttt atcagaaacg ctggtgaaat cagcggtttc accacgagga tcgatatcac
    15061 caattttaaa accaccaacc actgccaggc cgtcgttcaa caagccacgc accatccccg
    15121 tcaacggtgc tttaatttca tgctcaccaa tccaggcaat cacatcgccc tctttcacca
    15181 gatcgcctaa tttcacgttg gatcgcataa tgcctgcagc aggagcacgg atcacccgtc
    15241 gggtggtatg ccccataata ttgccaggaa catcggtatt ctcctgcgca caaccagagt
    15301 aaatcacctg accgagccag tgcccgcgat ttgtttcaat taccgcatga caatccttcc
    15361 ctgcagtaaa gcccggccca agcgcgattg ttactggtgc catatctgcc cgcgttccca
    15421 aattctgttt cgccagaata gcgtccacca cgcaaagcgg tttcagttca tcaagcaatg
    15481 aacaggcggg atctaccatc acagggatga atccgcgttc ggtaagtttc atcgcttccg
    15541 cagagctggt tgccaggcga gcagtgacgc cttcgaccgt catttcgcca tcgaacacgg
    15601 cctgggcaaa cgccacggta caacgaatca ctgtcggttt ttccacttcc agcatgatca
    15661 ctttaaaacc cgcatgatac agacgtagcg ccacaccact ggcgatatct cccgcaccgc
    15721 gaatcaccac cagctggtgt ccggaaggat gagaaggttt catcatcagc ccacccggcg
    15781 cgttattttt cacccataat atttctgcca gcacgctgat ggcgatctcc tgcggcgttt
    15841 ccgcacctat gttgtaacca acgggcgcat gtaaacgggc aatatgttct tcagccacgc
    15901 ctttctcacg caattgacgc aggaaaagct gaaccttgcg gcgacttgcc agcaacccca
    15961 accatgcaat gggctgttca atgagtttgt cgagggcttc acgatcctga ttattcgtgg
    16021 caatcaggac aaaattatca gggcgaatat ccagttcttc cactgccgca ccaaacgact
    16081 cagcatggag aagcgtggtt gatggtggga atagttcagg attgaggctt tcgcgataaa
    16141 tatcggcaac ggcaatatca aatcctaata gcgccgcact ctgggctatc gcccggttga
    16201 catgccccgc gccaatcaac accagacgtg gacgcatacc atggacgctg ataaatactg
    16261 acattgcacc tccacaatct gacccgacag catccgcacc gttacgagcc atacgtccat
    16321 ggaataatcg cggcttacgt tcctgcaatg cctgaagtga ctcttcaatc accttccgct
    16381 caaccattcc gccaccaatt gtaccgacga tagaaccatc ggcgcgcact aacatttgtg
    16441 cagaatggcg gggagtcgag cctcggctat caacaatttg cgccatcgca aacggacaat
    16501 tttgctcttc gagttttgca gcctctgtga aaatattcat accaacctca atggatatcc
    16561 tttcagtaac ccggaatacc cgggccgcta attcacaaat ctgcgcgcga ttgcaggatg
    16621 ttcttgtata tcgcccagcc agattgcctc tacgtcgtgt tgttgcagcg gttgaaggag
    16681 ctcgctttgt gcaatcgcat tctcacattg agaaaaacgg ttaatgaacc agactcgccg
    16741 acaaccttgc ggtacgtttt taaacgcccc ctgagggtgg cgaaccagcg caacgagatc
    16801 gctcagttgc aaggttgcat caggtgttaa cccggtaata tcagcaaact gcgaccagcg
    16861 atggacattt tctgtgctca ctttcgcgcc caaagtatgt cctcccatca cagcaatcac
    16921 gcagcaactg cttttgggta tgcaaggttc gtgctcatca ggcgctttta acggcattcc
    16981 acgcgagcca tctgcttcaa tgagaattac gtcacactct ggtcgttgca ccagtgcatc
    17041 aatcgcttct ggcgtaaatc cctgcacttt tccctggttc gctttccagc tgtgaaaaca
    17101 aaatgaaatg ggggatgtaa gagacgcatg aggaagcatg gcgggatcac gacagaaaac
    17161 cacgggccag tgagacgttg gcataaacat atgtgtcgtc gtcgtgatta acacacgcct
    17221 gccgctagcc tggagcagtt ctgccagcca aaaaagcaag ctggttttcc ccccagcacc
    17281 aacaacagaa atcacagtgg gacgtttctg cgcacctaaa tcaatgacta atgccgatgg
    17341 gtcaactata cttttcacga gtctttatga cctctctggg ataaattatc cccaacttaa
    17401 tccatcagga agtaacgcaa ttatcaggcg ttattagccc ctataaataa tggaaccact
    17461 atgtcagcca tcgactgtat aattaccgct gccggattat catcaagaat ggggcaatgg
    17521 aaaatgatgt tcccctggga acagggaaca attcttgata caagtatcaa aaatgcgttg
    17581 cagttttgta gccgaattat tttagtcacc ggctatcgtg gtaatgaact gcacgaacgt
    17641 tatgcgaacc agagcaatat cactattatt cacaacccag attatgcgca gggtttacta
    17701 acatccgtca aggccgcagt acccgcagtg caaacagaac attgttttct cacccacggt
    17761 gacatgccaa ccctcaccat cgatattttt agaaaaatct ggtcgttacg aaatgatggc
    17821 gcaatactgc cactccataa tggcatcccc ggccatccga ttttagtgtc aaaaccatgc
    17881 ctgatgcagg caatccagcg acccaatgtc accaatatgc gtcaggcact ccttatggga
    17941 gatcattatt ccgtcgaaat agaaaatgca gaaataattt tagatattga tactccggac
    18001 gactttatta cagcgcaaaa aaggtatact aaaatttaga aaatgtagtt aaacgattgc
    18061 gttcaaatat ttaatctctc cagcactctt tatcataatg ataaggaatc actgattttg
    18121 agaaaagaga aaaagtgata aatgggctga caataataat tttcaggagt gattttaatt
    18181 ctcatttggc acctgtgagt tcattcacaa aaagccttta agtttcgagc cacctcacat
    18241 ttttttatat ttcccgccaa acctggcaag agtggtgcga ttgttgctct atccccctaa
    18301 accaccggat ttctcaacac cggtcactca atgatatctg tataagctaa ggagagggtt
    18361 atgggggata ttatgcgtcc cattccgttt gaggaacttt tgacgcgcat atttgatgaa
    18421 taccaacaac aacgctcaat ctttggtatt cccgagcaac agttttactc acctgtaaaa
    18481 ggtaaaactg ttagcgtctt cggtgaaacc tgtgccactc ccgtcggccc tgccgctggc
    18541 ccgcacacgc agctcgcgca aaatattgtc acttcctggc tgactggcgg acgcttcatc
    18601 gaactaaaaa ccgtccaaat tcttgaccgc ctggagctgg aaaagccctg tattgatgcc
    18661 gaagacgaat gctttaacac cgaatggtct accgaattta ccttgcttaa agcctgggat
    18721 gaatacctca aagcctggtt tgccctgcac cttctcgaag cgatgttaca gccttctgat
    18781 tccggtaaat cgttcatctt taatatgagc atcggttaca acctcgaagg tattaagcaa
    18841 ccgccgatgc aacagttcat cgacaatatg atggacgcat ctgaccatcc gaaattcgct
    18901 caatatcgcg atacgctgaa taaattactc caggatgacg catttttagc ccgccacgga
    18961 ttgcaggaaa aacgcgaaaa cttgcaagcc ttacccgctc gcatccccac cagtatggtg
    19021 cagggcgtcc ccctctccac catgcacggc tgtcctccgc atgaaatcga agccatttgc
    19081 cgctacatgc tggaagaaaa agggctcaac acctttgtca aacttaaccc gaccttactg
    19141 gggtacgcgc gtgttcgtga gatcctcgat gtctgcggtt tcggttacat aggcttaaaa
    19201 gaagagtcat ttgatcacga cctcaagctg acgcaagcac tggaaatgct ggagcgcctg
    19261 atggtactgg caaaagaaaa atcactcggc tttggcgtaa aactgactaa cactctcggc
    19321 accatcaaca ataaaggcgc actgcctggt gaagagatgt atatgtcagg ccgtgcgctg
    19381 ttcccgctct ccatcaatgt tgcagcagtt ctctctcgcg cctttgacgg caaactgccc
    19441 atttcttatt ccggtggtgc cagtcagctg actatccgcg atatttttga tacaggtatt
    19501 cgccctatta ctatggcaac cgacctgctg aaacctggcg gctatctgcg cttaagtgcc
    19561 tgcatgcgcg agctggaagg ctccgacgcc tggggacttg accatgttga cgtcgaacga
    19621 ctgaacagac tggcagcaga cgcgttaacc atggaataca cccagaaaca ctggaagcca
    19681 gaagagcgta ttgaagtggc agaagacctg ccgctgaccg attgctacgt tgccccctgt
    19741 gttactgcct gcgctatcaa gcaagatatt ccggaataca tccgtctgct tggcgaacac
    19801 cgctatgccg acgcgctgga actcatctac caacgcaacg ccctacctgc cattaccggt
    19861 catatttgcg atcaccagtg ccaatacaac tgtacccgcc tggattacga cagtgcgctg
    19921 aatatccgcg aactgaaaaa agtcgcgctg gaaaaaggtt gggatgaata taagcaacgc
    19981 tggcacaaac ccgccggttc tggttcacgc catccggttg ccgtgattgg tgcaggtccg
    20041 gcgggtctgg cagcaggtta cttccttgcc agagcgggcc atccggttac gctgtttgaa
    20101 cgcgaagcca atgctggcgg cgtggtgaaa aatatcattc ctcagtt
//
LOCUS       DAJTVK010000075        20010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000075 DAJTVK010000000
VERSION     DAJTVK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.075"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..501
                     /gene="radC"
                     /locus_tag="OO372_002686"
     CDS             <1..501
                     /gene="radC"
                     /locus_tag="OO372_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6342139.1"
                     /translation="GLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESP
                     LLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIRE
                     AIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSF
                     AERGWI"
     gene            718..954
                     /gene="rpmB"
                     /locus_tag="OO372_002687"
     CDS             718..954
                     /gene="rpmB"
                     /locus_tag="OO372_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6342140.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            975..1142
                     /gene="rpmG"
                     /locus_tag="OO372_002688"
     CDS             975..1142
                     /gene="rpmG"
                     /locus_tag="OO372_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6342141.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1240..2049
                     /gene="mutM"
                     /locus_tag="OO372_002689"
     CDS             1240..2049
                     /gene="mutM"
                     /locus_tag="OO372_002689"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6342142.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2088..2567)
                     /gene="coaD"
                     /locus_tag="OO372_002690"
     CDS             complement(2088..2567)
                     /gene="coaD"
                     /locus_tag="OO372_002690"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6342143.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2575..3852)
                     /gene="waaA"
                     /locus_tag="OO372_002691"
     CDS             complement(2575..3852)
                     /gene="waaA"
                     /locus_tag="OO372_002691"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6342144.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4265..5323
                     /gene="rfaQ"
                     /locus_tag="OO372_002692"
     CDS             4265..5323
                     /gene="rfaQ"
                     /locus_tag="OO372_002692"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6342145.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5320..6444
                     /locus_tag="OO372_002693"
     CDS             5320..6444
                     /locus_tag="OO372_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6342146.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6437..7234
                     /gene="rfaP"
                     /locus_tag="OO372_002694"
     CDS             6437..7234
                     /gene="rfaP"
                     /locus_tag="OO372_002694"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6342147.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7250..8266
                     /gene="waaO"
                     /locus_tag="OO372_002695"
                     /gene_synonym="rfaI"
     CDS             7250..8266
                     /gene="waaO"
                     /locus_tag="OO372_002695"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6342148.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNFKQNEPINGIMNYIYYFYL
                     KIIK"
     gene            8283..9278
                     /locus_tag="OO372_002696"
     CDS             8283..9278
                     /locus_tag="OO372_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR6342149.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            9288..9980
                     /gene="rfaY"
                     /locus_tag="OO372_002697"
     CDS             9288..9980
                     /gene="rfaY"
                     /locus_tag="OO372_002697"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6342150.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            10006..11031
                     /locus_tag="OO372_002698"
     CDS             10006..11031
                     /locus_tag="OO372_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR6342151.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            11116..12099
                     /locus_tag="OO372_002699"
     CDS             11116..12099
                     /locus_tag="OO372_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6342152.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            12145..13398
                     /locus_tag="OO372_002700"
     CDS             12145..13398
                     /locus_tag="OO372_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6342153.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNNLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(13468..14439)
                     /gene="rfaC"
                     /locus_tag="OO372_002701"
     CDS             complement(13468..14439)
                     /gene="rfaC"
                     /locus_tag="OO372_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6342154.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(14443..15489)
                     /gene="rfaF"
                     /locus_tag="OO372_002702"
     CDS             complement(14443..15489)
                     /gene="rfaF"
                     /locus_tag="OO372_002702"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6342155.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(15499..16431)
                     /gene="rfaD"
                     /locus_tag="OO372_002703"
     CDS             complement(15499..16431)
                     /gene="rfaD"
                     /locus_tag="OO372_002703"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6342156.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            16645..17841
                     /gene="kbl"
                     /locus_tag="OO372_002704"
     CDS             16645..17841
                     /gene="kbl"
                     /locus_tag="OO372_002704"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6342157.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            17851..18876
                     /gene="tdh"
                     /locus_tag="OO372_002705"
     CDS             17851..18876
                     /gene="tdh"
                     /locus_tag="OO372_002705"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6342158.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTVICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            19115..>20010
                     /gene="waaH"
                     /locus_tag="OO372_002706"
     CDS             19115..>20010
                     /gene="waaH"
                     /locus_tag="OO372_002706"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCR6342159.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         5398 a         4362 c         4727 g         5523 t
ORIGIN      
        1 ggcttgttaa cctctgaata tgaacaattt agtggcgttc atggaattgg cgtagcgaaa
       61 tttgcccagt taaaggggat tgctgaactg gcgcggcgtt actacaacgt gcggatgcgt
      121 gaagaaagcc ctttactcag cccggagatg acgcgggaat ttttacaaag ccagctcacg
      181 ggtgaggagc gggagatctt tatggtgatc tttctcgact cacaacaccg ggttataacg
      241 catagccgtc ttttttccgg cacgctaaac catgttgaag tccatcctcg ggaaattatc
      301 cgggaagcga taaaaataaa cgcctcggcg ctgatccttg cacataatca cccttcgggt
      361 tgtgctgaac ccagtaaagc ggataaactc attactgaac ggataataaa gagttgtcag
      421 ttcatggatt tacgcgtgct cgaccatatc gtgattgggc gtggagagta tgtttctttt
      481 gccgaacgcg gctggattta acccgctatg cgcgatcctt cgggatcttt gtctgttcgg
      541 gacttgagca catcgctgag tcagcgtata ctacgccacc tttgagaatc tcgggtttgg
      601 catttgggcc tggcaatcga gagttcacat agaactgcga tgaccgggct gtaaagcctg
      661 acgaggcgcc aataccccat acgaagctcg agctaatttg atttttggag aatagacatg
      721 tcccgagtct gccaagttac tggcaagcgt ccggtgaccg gtaacaaccg ttcccacgca
      781 ctgaacgcga ctaaacgccg tttcctgccg aacctgcact ctcaccgttt ctgggttgag
      841 agcgaaaagc gttttgtcac cctgcgcgta tctgctaaag gtatgcgtgt aatcgataaa
      901 aaaggcatcg atacagttct ggctgaactg cgtgcccgtg gcgaaaagta ctaagtactt
      961 agaggaaata aatcatggct aaaggtattc gtgagaaaat caagctggtt tcttctgctg
     1021 gtactggtca cttctatacc actacgaaga acaaacgtac taagccggaa aaactggaac
     1081 tgaaaaaatt cgatccagtt gttcgccagc acgtgatcta caaagaagcg aaaatcaaat
     1141 aattctcgct ttgatgtaac aaaaaacctc gctccggcgg ggttttttgt tatctgcttg
     1201 cccccatatt gattgcatct gttcattcct ggagatgcta tgcctgaatt acccgaagtt
     1261 gaaaccagcc gtcgcggcat agaaccgcat ctcgttggtg caaccattct tcatgcggtg
     1321 gtgcgcaacg gacgcttgcg ctggccggtt tcagaagaga tctaccgttt aagcgaccaa
     1381 ccagtgctta gcgtgcagcg gcgggctaaa tatctgctgc tggagctgcc tgagggctgg
     1441 attatcattc atttggggat gtctggcagc ctgcgcatcc ttccagaaga acttccccct
     1501 gaaaagcatg accatgtgga tttggtgatg agcaacggca aagtgctgcg ctacaccgat
     1561 ccgcgccgct ttggtgcctg gctgtggacc aaagagctgg aagggcacaa cgtgctggct
     1621 catcttggac cggagccgct tagcgacgat ttcaatggtg agtatctgca tcagaagtgc
     1681 gcgaagaaaa aaacggcgat taaaccgtgg ctgatggata acaagctggt ggtaggggta
     1741 gggaatatct atgccagcga atcactgttt gcggcgggga tccatccgga tcggctggcg
     1801 tcatcactgt cgctggcaga gtgtgaattg ttagctcggg tgattaaagc ggtgttgctg
     1861 cgttcgattg agcagggtgg tacaacgctg aaagattttc tgcaaagtga tggtaaaccg
     1921 ggctatttcg cgcaggaatt gcaggtttac ggacgaaaag gtgagccgtg tcgggtgtgc
     1981 ggtacgccga ttgtggcgac taaacatgcg cagcgggcaa cgttttattg tcggcagtgc
     2041 cagaagtaat tcatacgcgc cggatggcat accatccggc ataaacgcta cgctaacttc
     2101 gccaccagcg cctgatggac attctccggc aggaaatggg tgacatcgcc ctgatggcgc
     2161 gccacctctt tcaccaacga tgaagagata aacgaccact ctttcgacgg catcagaaac
     2221 acactttcca gttccggcat taagtggcga ttcatatgcg ccagctgcat ttcatattca
     2281 aaatccgcca ccgcacgcag gccacgaatc agcaccgtag cgtgttgatt acgggcgaag
     2341 ttcgccatca aatcactaaa cccaaccact tccacgttcc ccagatgcgc ggttgcctgc
     2401 tgtgtcagcg ccacacgctc ttccagggta aacatcggtt ttttactggg gctggcggca
     2461 atcgccagaa taacgtgatc gaacatttgc gtggcgcgcg tcacgatatc gatatgacca
     2521 ttggtaatgg gatcgaaagt acccggataa atcgcccgtt tttgcataac aacctcaatg
     2581 cgttttcggt ggcaggtaag gttccagcag ttgaagcaga cgctgtagcg cgccctggtt
     2641 ttgatacagt acttcaacgg catgacggcc atagaaacta cggtaatcgg cgtcggtgag
     2701 taaagaggaa acctcttttg caagcgtagt ggcatcggta acggtaatca gcccgcttgc
     2761 ctgctccagc cgcgcgcaaa tgtctttgaa attaaaagta tgcggcccca tcaataccgg
     2821 aatagcgtgt gcggcagctt ccagaggatt atgccctcca cgttcaacca gtgaaccgcc
     2881 aacaaaggcg agatcggcaa tgccgtacag taacatcaac tcgcccatcg tatcgccaac
     2941 cacaacctgc gtgctggtgg aggggacttc ccctgaagag cgtgtgatat agcttagtcc
     3001 agcctggcgg acaaggttaa tcgcatccgg gaagcgttct ggatgacggg gtaccaggat
     3061 gagcaataaa ttcgggaatt gctgtaataa tgcctgatgt gcggcgatca ccacactctc
     3121 ttcaccttcg tgagtgctgg tggcaatcca taccgggcgg tgtggtgccc actggcggcg
     3181 cagcgtcacg gctttagcag ccaactgcgg cgttacagaa atatcgaatt tcaggctacc
     3241 ggtaacggtt acctggttat tttttgcgcc cagcgccaca aaacgtgcac catcttcttc
     3301 attttgcgca gcaatcagcg taatacgacg cagtaagcgg cggacgaatt tacccagttt
     3361 ggcataacct gcggccgagc gggcagagag tcgcgcgtta gcgatcacca gcggaatttt
     3421 acgtttatgt agcgccgcaa tcaggttagg ccatagttcg gtttccataa tcaacaccag
     3481 tttggggtcg actttattca ggaaacggtt gagcgcatcg ggcaggtcat acggcagata
     3541 aacgtgctga acatccttcc cgaaagccga ttgtacgcgc tccgaaccgg ttggcgtcat
     3601 ggttgtaacg gtaatcggta aatcaggata acgatggcgc agcgcgcgca ccaacgggat
     3661 cgccgccaga gtttcaccga cggagacgga gtgcagcata atgccgcctg gttttagcgg
     3721 atggcggtaa aaaccgtaac gttcacccca gcgttttcga taggccggag ccttacgtcc
     3781 gcgcacccag agccgtatcc agatcagcgg ctgaataagg tagagaaggg cggtgtaaag
     3841 caattcgagc atagtaaata gctgacttat ggatgtgctg gggattctat gtatttagct
     3901 gtggctttac cattactttt cccgtttttg acttaaatag caatttaccc gccaattctt
     3961 gctgaacatc tatacttttt ttgtattttt atgcgattca ttgatttctg ctgtacgtgc
     4021 aggaaggaat cgcagagcaa cagtgaaaga atatggttgc taatgatttt atttgattga
     4081 tttctttatt gaaaatcaac aatgtgctta aaaagcattc gggtcggatt gccgcgaaag
     4141 aagtgataca ctagcgcgtc aatatcagtg cggtatcagt attcaggtag ctgttgagcc
     4201 tggggcggta gcgtgctttt tctgcttcac cttaatcgga taatctcaac aaaaagagtt
     4261 acttgtggat aagccatttc gaagaatttt gctcattaag atgcgttttc atggggatat
     4321 gttattaact actcccgtca ttagttcgct gaaaaaaaat taccctgacg caaaaatcga
     4381 tgtgctgctt tatcaggaca ccaccccgat cctgtctgaa aatccagaga ttaacgcgct
     4441 ctacggcata aaaaataaaa aagcaaaagc ctcagaaaaa attgccaact tttttcatct
     4501 catcaaggta ttacgtgcca ataagtatga ccttatcgtc aatcttaccg atcaatggat
     4561 ggttgctata ctggttcgct tattaaatgc ccgtgtgaaa atttcccagg attatcatca
     4621 tcggcagtct gctttttggc gtaaaagttt cacccattta gtgccgttgc agggtggaaa
     4681 tgtggtggaa agtaacttat ccgtgctgac accattggga cttgattcgt tggtgaagca
     4741 gacaaccatg agttacccgc ctgcaagctg gaaacgtatg cgtcgcgaac ttgatcacgc
     4801 tggtgttgga caaaattatg tggttatcca acctacggcg cggcaaatct tcaaatgctg
     4861 ggacaacgcc aagttttccg ctgtgattga tgccttacat gctcgtggtt atgaagtcgt
     4921 tctgacgtcc ggcccagata aagacgatct ggcctgcgtc aatgaaattg cgcagggatg
     4981 ccagacgcca ccagtaacgg cgctggctgg aaaggtgacc ttcccggaac ttggtgcgtt
     5041 aatcgatcat gcgcagctgt ttattggcgt tgattccgca ccggcgcata ttgccgctgc
     5101 agttaatacg ccgctgatat cgctgtttgg tgcgacagac catattttct ggcgtccttg
     5161 gtcaaataac atgattcaat tctgggcggg agattaccgg gaaatgccaa cgcgcgatca
     5221 gcgtgaccga aatgagatgt atctttcggt tattccggcg gcagatgtca ttgctgctgt
     5281 cgataaatta ctgccctcct ccacgacagg tacgtcgtta tgatcgttgc tttttgttta
     5341 tataaatatt ttccctttgg cggtttgcag cgcgatttta tgcgtattgc tcagacagtc
     5401 gccgcccgag gtcatcatgt tcgggtttat acccagtcgt gggaaggcga atgccctgat
     5461 gtatttgaac tgatcaaagt gccggttaaa tcgcatacca atcacgggcg caatgcggag
     5521 tattttgcct gggtgcaaaa acatttacgc gaacatcccg tcgataaagt cgttggattc
     5581 aacaaaatgc cggggctgga cgtttattat gccgctgatg tttgttatgc cgagaaagtc
     5641 gcgcaggaaa aaggcttttt ctatcacctg acgtcacgtt atcgccatta tgccgccttt
     5701 gagcgggcaa ccttcgaaca gggcaagccg acacagctgc tgatgctgac agataagcaa
     5761 atcgccgatt tccagaaaca ttatcagact gaagcggagc gttttcatat tctgccaccg
     5821 gggatttatc ctgatcgtaa atatagccag cagccagcta atagccgtga aatcttccgt
     5881 aagaagaatg gaataaccga acaacaatat ttattgttgc aggtcggttc agacttcacg
     5941 cgtaaaggtg tcgatcgttc cattgaagca cttgcttcgt taccggattc gctgcgccac
     6001 aacacattgc tatatgttgt tggacaggat aagccgcgaa aatttgaggc gctggcagaa
     6061 aaacgcggcg tgcgcagtaa tgttcacttc ttctcggggc gcaacgatgt ctcggaatta
     6121 atggcggcgg cggatttatt actgcatcct gcctaccagg aagcagcggg aattgtgcta
     6181 ctggaagcga ttactgcagg attaccggta ctaacaacag ccgtttgtgg ctatgcgcat
     6241 tatattgtcg acgccaattg cggcgaggct attgctgagc cattccgcca ggaaacattg
     6301 aatgagattt tacgcaaagc gttaacgcaa tcttcattgc gccaggcctg ggcggaaaat
     6361 gcgcgacatt atgctgatac acaagattta tacagtctgc cagagaaagc ggcggatatc
     6421 ataacgggtg gtctggatgg ttgaacttaa agagccgttt gccacgttat ggcgcggtaa
     6481 agatcctttt gaggaagtta aaaccttgca gggtgaggtt tttcgtgaac tggaaactcg
     6541 ccgcactctg cgatttgaaa tggcgggcaa aagctatttt ctcaaatggc atcgcggcac
     6601 gaccctgaaa gagataatca aaaatttact ctcattgcgg atgccagtat taggcgcaga
     6661 ccgcgaatgg aatgcgattc atcgactgcg ggatgtcggc gttgatacta tgtatggggt
     6721 ggcatttggc gaaaaaggta ttaatccgct gagcagaacc tcgtttatta ttaccgaaga
     6781 tctgacccca accatcagtc tggaagatta ctgtgctgac tgggcgacca acccaccaga
     6841 tgttcgcgta aagcgtatgc ttattaagcg tgtcgcgacg atggtgcgcg atatgcatgc
     6901 tgtgggcatt aaccaccgtg actgttatat ctgtcatttc ctgctgcact tgcctttttc
     6961 cggtaaggaa gaggagttaa aaatttcggt aattgacctg caccgggcgc agcttcgcac
     7021 gcgcgttcca cgtcgttggc gcgataaaga tcttattggg ctttattttt cttcgatgaa
     7081 tatcggcctg actcagcggg atatctggcg gtttatgaaa gtgtattttg tcgccccgct
     7141 taaagacatt ctcaagcagg aacaaggact gctgtcgcaa gcagaagcaa aagccacaaa
     7201 aatcagggaa agaacgattc gaaaatcgtt gtaatttccg gagagaaata tgagtgccca
     7261 ctattttaat ccacaagaga tgatcaataa gacaatcatc ttcgatgaaa ggccagcggc
     7321 gtcagtggca tcatcattcc atgttgctta tggcattgat aaaaactttc tttttggttg
     7381 tggtgtttca atcacgtcag ttttgttaca taacaacgac gtgagttttg ttttccacgt
     7441 ttttattgat gatatccctg aagccgatat ccagcgttta gcccaattgg cgaaaagcta
     7501 tcgtacctgt atccagatcc atctggtaaa ttgtgaacgg cttaaggcat taccgacgac
     7561 caaaaattgg tctattgcca tgtatttccg ttttgtaatt gcagattact ttattgatca
     7621 acaagataag atcctttacc tggatgctga tatcgcctgt cagggaaact taaagccgct
     7681 gataacaatg gatcttgcca ataacgttgc tgctgttgtt actgaacgcg atgctaactg
     7741 gtggtcgtta cggggtcaaa gtctgcagtg taatgaactt gaaaagggtt actttaattc
     7801 aggtgtcctg ttaattaata cactagcgtg ggcgcaggag tccgtttctg ctaaagcgat
     7861 gtcgatgctt gctgataaag ccgtcgtttc ccgtttaacc tatatggatc aagatatact
     7921 taatcttatc ctgtcaggga aagttaaatt cattgatgct aaatacaata ctcaatttag
     7981 tttaaattat gaattaaaaa aatcatttgt ttgtccaatt aatgatgaaa ccgtattaat
     8041 tcattatgtc ggcccgacaa aaccctggca ttactgggcc ggttatccaa gtgcgcaacc
     8101 ttttatcaac gccaaagaag catcgccctg gaaaaatgaa ccgtttatgc ggccaattaa
     8161 ttcaaactat gctcgttatt gcgccaagca taattttaaa caaaatgaac caattaacgg
     8221 gataatgaat tatatttatt atttttattt aaagataata aaatgatatt gagaaaagtt
     8281 aaatgaatga atttataaaa gaacggtttt cgtatttagc agataataaa aaagaaaacg
     8341 ccccagaact aaatgtttcc tacggtatcg ataagaattt tttgtatggt gctggcgttt
     8401 caatttcttc cgttttgatt aataattcag atattaattt tgtctttcat gttttcactg
     8461 attatgtgga tgatgattat ttaaagtcat ttaatgaaac agcaaaacaa tttaatacct
     8521 caattattgt atatttaatt gacccaaaat actttgctga tctgccgacg tcacagtttt
     8581 ggtcgtacgc gacatacttc agggtattgt cctttgaata tctgagtgaa agtatttcca
     8641 cactgctgta tctggatgcc gatgtcgttt gtaaaggaag tctgaagcct ctcacagaaa
     8701 ttatatttaa agatgagttt gctgcggtca ttcctgacaa tgttagtact caggcggcat
     8761 gtgcaaaacg cctcaacatt cccgagatga atggacgtta tttcaatgca ggcgttatct
     8821 atgtcaatat taaaaaatgg catgaagcaa atttgacacc gtatttactc acgcttttac
     8881 gaggggaaac taaatatggc tctcttaaat atttagatca ggatgcgttg aatatcgcat
     8941 ttaatatgaa taatatctac ctcgcgaagg attttgatac tatttatacc ctgaaaaacg
     9001 aacttcatga tcgtagtcat cgaaagtatc agcaaaccat taccgataaa acagtattga
     9061 ttcactatac agggataact aaaccatggc atagctgggc tggttatccg tctgcatcat
     9121 actttaatat cgcgcgtgaa caatctccct ggaagaaata tcctcttaaa gaggcgcgga
     9181 ctgttgcaga aatgcagaaa caatataagc atctgtttgc ccatggtgag tatattaaag
     9241 gcataacttc attaattaag tacaagctta agaaataaat tactcttatg attacaagta
     9301 tacgctatcg cggcttctca ttttattaca aagataatga taataaatat aaagaaatct
     9361 ttgatgaaat actggcctat aattttaaaa cagtaaaagt attacgtaat attaatgata
     9421 ccaaagtatc attgattgat acgaaatatt gtcgctatgt ttttaaagtc tttgccccga
     9481 aaacgaaaag gaatgaacgt tttttaaaat cttttgtaaa aggtgactac tatcagaatt
     9541 tgatcgttga aaccgatcgt gtcagaagtg caggtcttac ttttcctaat gatttttatt
     9601 ttctggctga gcgtaaaatt ttcaactatg ccagtgtttt tatcatgctt atcgagtatg
     9661 tcgagggcgt agagctcaat gatatgccga tcattccaga agatgttaaa gcagaaatta
     9721 aggcttcaat ggaaaaattg catgccttaa atatgctatc gggcgaccca caccggggta
     9781 actttatcgt tagtaaagat ggtgtcagga taattgacct atccggtaaa agctgtacgg
     9841 cagagcgtaa agctcgtgat cgtctggcca tggaacgtca cctgggtatt gccaacgaaa
     9901 taaaagatta tggctactat tcagtaatct acagaaccaa actacgcaaa tttattaaaa
     9961 aattaaaagg caaagcgtaa accacacagt caaaacggaa ccaacatgga tttattagct
    10021 gagagtatta ctgaagtcgc tgtctctggg gaaattgcta acaccgatcg tgtgttaaat
    10081 atcgcttacg gtattgaccg taacttttta tttggtgcgg cagtatctat gcaatcagtt
    10141 gttatgcata atccggacct tgcggttaag tttcatctct ttaccgacta cattgatgaa
    10201 gattatctac aacgtgttaa tgcttttacc agcaaaaatg ctaacgttga agtaagaatt
    10261 tataaagtct ccagtgcctt tattgatatc ttccccagcc tgaaacagtg gtcttatgca
    10321 acattcttcc gtttagttgc gttccagtat ctgagtgaaa ctattgaaaa tctgttatat
    10381 atcgatgctg atgtcatctg taaaggctca ttagctggat tgcttgatat taattttgat
    10441 ggggataagt ttgcagctgt tattaaagat gtgcctttta tgcaggaaaa accagcgaag
    10501 cgtctggcta tagagggact tccagggaat tatttcaacg ccggtgtagt atatctgcag
    10561 cttgaagcat gggcgaaaaa tgattttatg aataaagcca ttgctatgct ggcaagtgac
    10621 ccgcagcaca cgaagtataa atgccttgat caggatattt taaatattct gttctttggt
    10681 cattgtattt ttattagcgg cgattatgat tgcttttatg gcatcgatta tgagttaaaa
    10741 aataaaagcg atgaagatta taaaaagacc attaccgatg atactaagct gattcattat
    10801 gttggcgtaa cgaagccctg gaacgactgg acgaattatc cctgccagaa gtattttaat
    10861 gaggcttatc aggcttcttg ctggaatgat gtggcgttta ttccagccac gaatgaaaag
    10921 cagtatcaag tgaaatatca acatgcaaag aaaaatggtg atacgtttaa cgcttttatt
    10981 tactttatta aatttaaatt aaataagtat aaaagaaaac tatttggata ataataaaga
    11041 tcatggatgt ctgagttaca ataataatta aggatgagta ctatcatatt ttattagttc
    11101 aggcaagaga acacgatgag caatgattac cctttagtat ccataataat accgacgtat
    11161 aattcatctg attacattac tgaaactcta acgaaattag aaaaacaaac ttacccaaat
    11221 tttgaaattg ttattgttaa tgatggttct aaagataaca catcaaacgt tttgagagag
    11281 tatgggttaa cccactctcg attaattatt atcaataaag aaaatggcgg tgtttcgtct
    11341 gccaggaata caggtatccg caaggcgcaa ggacagttta tatgttttat ggatgatgat
    11401 gatgagatag atcctaacta tctgctgaag atgtatacca gacaacatga gacgggaggg
    11461 gatgcgattt attgtgggct ttatggccat tatataaaag atggtgttac ttactcacct
    11521 ataaatacag agtttaatga aggatcttta cttttcgact ttttttataa aaaggttaga
    11581 ttccatatag ggtgcttgtt tataagaaaa caacttctgg aagagaataa tctttttttt
    11641 gatgaagatt tacgactagg agaagatctg gattttatct atcgactgct aattacatgc
    11701 gatatgtatg cggttccata ttatatgtat aagcataact atagagaaaa ttccttaatg
    11761 aactcatgta gaaccatcac tcattatcga catgagtcat ttgcgcacga aagaatctac
    11821 tcttctgtga tgcagttata caaaggtaac aggaaagaag aaattcatac attattgagt
    11881 caaaatagag cttatcataa aactcgttat ttatggaatg ttctacttaa tggtgatttt
    11941 gaactattaa atcaattagt tgaaagcaat gaaaaagaat tgaatgattg taatctttct
    12001 ggcaagagag ataaaagacg agcaaaaata ttagcatcaa aaaattatat tctctggagg
    12061 atggtaagac tggtaaatag gaaaaagaat aaacgttaga tcttgcatta aataatccgt
    12121 cctcgtagca taggttgaaa ctatatgtca ttttgttgga atgaaattaa ttctggtata
    12181 aagtctttaa ttctcatatt atgtattttt tctttgatga ctttgtcttt atgggatgat
    12241 gttgcaacaa agtttcttca tgcagctgga attatatctg cattgtattt tctaataaca
    12301 ccaaaaaaaa caataactaa caatcctact ttgttaattt tcatctcatt atgtcttttg
    12361 ggaatcataa atatcatctg gtattcacat tataaagttt caggctctgt ttataccaat
    12421 gcatatcgtg gcccaatgga aactgggaaa attgccttgt gtagtgcttt tattttcttg
    12481 gttctttttg ctaaaaatga gttgagaaca aaaataaaat ttgggaaact aattctgttc
    12541 gcatccctgg caacgcagtt actttttttt gcgcatgcca tgtggcaaca tttctattta
    12601 aacgttgacc gtgttgcatt atcagcttcc cacgctacaa cagcaggcta catcatcctt
    12661 ttcccttctt tactggcatc aattctcatt ttaaaatccg actttagaca taaaacaaca
    12721 ttatatacaa ttaacttcat gcttagctta tgtgctgtca tagtaactga aacgcgtgca
    12781 gccatattag tgtttccatt ctttgcgtta ctattaatcg taatggatag ttatattaat
    12841 aagcgaatta attataagtt atattgtttt attgcgattg cattattagc aggtgtattt
    12901 tcttttaaag atacattgct tatgagaatg aataacttaa ataacgattt agttaattat
    12961 tcgcatgata acaccagaac ttcagtcggt gcccgtctgg caatgtatga agttggctta
    13021 aaaacatatt ctccaatagg acaatcactg gaaaaacgag cggaaaaaat acatgaacta
    13081 gaagaaaaag agcctagatt gagtggcgct ttaccctata tagattctca tttgcataac
    13141 gatctcatag atacgttatc aacgcgtggt attcctggag ttgtattaac aattttagcc
    13201 ttttcagcaa tactcatata tgccttaaga actgctaaag aaccttatat tttaatcttg
    13261 cttttttcac tactggtagt aggcctaagt gatgtaatac tcttttctaa accggttccg
    13321 actgctgtgt ttgtcaccat aatattgctt tgtgcttatt ttaaagcaca atcagaccaa
    13381 tgtttattag ataaataatg aatatgtgaa ataaaatcag caaggaggct gattttattt
    13441 caaaattatc tattaagggg ttgtaattta aatcatagca acgttttctt caatgaatcg
    13501 cttaacagca tttgctgtca attgagacaa ctcattcccc ggagccctac aaaccatctg
    13561 attcttccca tatccaccaa ttaatcccgg atcggttggt ccataaaccg tgatattggg
    13621 tctatccagt gccgccgtta aatggcttaa ccccgtatcc accgacacta caaatttagc
    13681 cccggccagc acgcgggcaa cgccttccag actcatcttc ggcaatactt caacataagc
    13741 aaatccttcc gccagtcgtt tcgcccgctc ttcctcatgc ggcgcgcccc acggaagttt
    13801 aatccgtatt cctgaatcag ccagtaaacc aatcaattct cgccagtgtt cttccggcca
    13861 gtgtttatca tcacgggtcg tcgcatgaag aaatacggca tattcgccag catctgtagg
    13921 caggttcgtc aggaaatgct gtgcaatagc ataatcgccc tgggtttgcg gtttgctata
    13981 gcccagactt ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat
    14041 atgatgctta cgattgtaaa acagactggc taaaggttcg cgagcggttt gccagtccat
    14101 gccatgcttt acgccatgcg ccagacgcgt caccagcgcc gcgcttttta ccagcccctg
    14161 agcgtcgata actgcgtcat agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc
    14221 cgcttttatg ggggccgaga accaggcttt acgccagcga cgtattgcca caggaataac
    14281 tcgctcaacg gcggcgtgcc aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc
    14341 aaacttaatc cctgggattg cctgctgggc atcagtgagt gcgggcaacg tatggagaac
    14401 atcgcccatc gacgatgttt taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa
    14461 caatagcgcg ttgagttctt ccagtacgcg ctggggagta atgtcgatta agctctggtg
    14521 ataaccctcc gcagcgtcac ctttacgcac tttgtgatag ccggtaatca ggcggatcac
    14581 gcgcgcttta tgggatagcg gcggtgtgaa gtccgggcta ctcggaccat acagggcaac
    14641 cagcggacga ttgagcgccg ccgcaacgtg catcaggcca gaatcgttag tgacaatggc
    14701 tttacaggct gcaatcagga taaccgcttg atcaagctgt gtttcccccg ccaggttccg
    14761 acaccatgcc tgctgctcgg tattcaaagc ggcaagaatc tcattgcccg cttcatgatc
    14821 tttcgccgag ccaaacagaa ccacctgata accttcatca atcagctgct ttgccagttc
    14881 cgcatagtgg tagtgtggcc agcgttttgc cggaccaaac tccgcacccg ggcaaaaacc
    14941 aatcatcgga cgttctgatg aaagcgaaaa ttgattacag gtatatgatt tttcaccttc
    15001 gctcacctgc aattgcggcc ataacaacgg ttttggtaga tcttgtgcgg tacgcatcac
    15061 gcctttgtca taggccagcg cgacatagcg ttccaccatt agcggccagg cttctttatc
    15121 gagcacgcgt aaatcgttga gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg
    15181 aggaataccc gcgaagaaag gcactaatgc agatttgaag gagttgggta agacgtaggc
    15241 gcggtcgtag cgcttttcac gcaggctatg acccagtttg cggcgttcgc cgatttccag
    15301 cgctccgtga ccgagaggca taggaatagc ttcgttaact tccggcatcc gcgataataa
    15361 tggacggcac catgccggtg ccatcacatc gattatcgcc tggggatagc gcgcctggag
    15421 cgtgcgatag agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag
    15481 tattttcatg cagagctctt atgcgtcgcg attcagccag gccatgtatt ccgttacacc
    15541 ttcagcaacg gttttgaacg gtttgtcgta acccgccgcg cgcagatttg tcagatctgc
    15601 ctgagtgaac gcctggtagc ggcctttcag cttatccggg aacggaatgt attcgatctg
    15661 gcctttcttg tgataagcca gcgtagcatc agctacagcc tggaaggatt ccgcacgacc
    15721 agtaccgagg ttgaagatgc cggaaacgcc attttccagg aaccacagat tcacatcagc
    15781 cacgtcgccc acatagacga agtcgcgttt gaagttctcg ctaccttcaa acagcttcgg
    15841 tgattcaccg ttgttaagct gggtgttgag atggaaagcg acgctcgcca tgctgccttt
    15901 atggccttca cgcggtccat aaacgttgaa atagcggaag ccaacaatct gcgagttagc
    15961 ttcaggcagg atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacgtt
    16021 caacggtttt tcgtactcgc gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc
    16081 tgcggaagag gcatacagga acgggatttc gcgctccaga cagtagtgca gcagctcttt
    16141 ggagtattga tagttgttat ccatcatata cttgccatcc cactcggtgg tggaagagca
    16201 cgcgccttcg tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat
    16261 caggaagtct tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt
    16321 ggtgccgtct ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc
    16381 cttaacgatg ttgctgccga taaagcccgc gccgccggta acgatgatca taactgtaac
    16441 cttcgaatta tggagtcaga gacaatctca gacacgaata cttctatcat atcactacac
    16501 ggcgttggct tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt
    16561 tgcagaagtg ttaacgcgtc atctcgtcgc gacctataag tttgggtaat atgtgctgga
    16621 atttgccctg tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat
    16681 ctggaaaccg cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag
    16741 caagcagata tcactgtggc tgatggaagc cacgtcatta acttttgtgc caataactat
    16801 ctcgggctgg cgaattatcc ggatctgatt gcggcggcaa aggcgggaat ggattctcac
    16861 ggtttcggca tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt
    16921 gaacaaaaac tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt
    16981 gatgctaacg gtggcctgtt tgaaacgctg ctgggcgcgg aagacgccat tatctccgac
    17041 gcactgaacc acgcgtctat tattgatggt gtgcgtctgt gtaaagctaa acgctatcgc
    17101 tatgccaaca acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt
    17161 gcgcgtcatg tgctgattgc caccgatggt gtgttctcga tggacggcgt gattgccaac
    17221 ctgaagggcg tttgcgatct cgcagataaa tatgatgccc tggtgatggt agacgactcc
    17281 cacgcggtcg gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg
    17341 ggccgggtcg atattatcac cggcacgctc ggtaaagcgc tgggcggggc ttctggtggt
    17401 tataccgcgg cgcgcaaaga agtggttgag tggctgcgcc agcgttctcg tccgtacctg
    17461 ttttccaact cgctggcacc ggccattgtt gccgcatcca tcaaagtact ggagatggtc
    17521 gaagcgggta gcgagctgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa
    17581 atgtcggcgg cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt
    17641 ggtgatgcgg tagtggcgca gaaatttgcc cgtgagctgc aaaaagaggg catttacgtc
    17701 accggtttct tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct
    17761 gcggcgcata cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa
    17821 caactgggcg ttattgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa
    17881 gagggcatct ggatgaccga cgttcctgta ccggaactcg ggcataacga tctgctgatt
    17941 aaaatccgta aaacagtcat ctgcgggact gacgttcaca tctataactg ggatgagtgg
    18001 tcgcaaaaaa ccatcccggt gccgatggtc gtgggccatg aatatgtcgg tgaagtggta
    18061 ggtattggtc aggaagtgaa gggcttcaag attggcgatc gcgtttctgg cgaaggccat
    18121 atcacctgcg gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg
    18181 atcggcgtcg gcgttaaccg cccgggctgc ttcgccgaat atctggtgat tccggcgttc
    18241 aacgccttca aaattcccga caatatctcc gacgacctgg cttccatttt tgatcccttc
    18301 ggtaacgccg tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct
    18361 ggtgcaggcc cgattggtat tatggcagcg gcggtggcga aacacgttgg tgcacgcaat
    18421 gtggtgatca ctgatgttaa cgaatatcgt ctcgaactgg cgcgcaaaat gggtatcacc
    18481 cgtgcggtca acgtcgctaa agaaaacctt aatgatgtga tggcagaact gggcatggcc
    18541 gaaggctttg atatcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctc
    18601 gacaccatga accacggcgg tcgcattgcg atgctgggta ttccaccgtc cgatatgtct
    18661 atcgactgga ccaaagtaat ctttaaaggc ttgttcatta aaggtattta cggtcgtgag
    18721 atgtttgaaa cctggtacaa gatggcggcg ctgattcagt caggtctgga tctctcgccg
    18781 atcattaccc atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg
    18841 ggccagtccg ggaaagtaat tctgagctgg gattaacacg aacaagggct ggtattccag
    18901 cccttttgcc tgaggataat ccgttaaata tgtaaaatcc tgtcagtgta ataaagatat
    18961 cgtaattgcg ctgatctctt atatagctgc tctcattatc tctctaccct gaagtgactc
    19021 tctcacctgt aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa
    19081 acgtcaggat aacttcagag gtcgtcggta atttatgatg aacagcacca ataaacttag
    19141 tgttattatt ccgttatata atgcgggcga tgatttccgc acttgtatgg aatctttaat
    19201 tacgcaaacc tggactgctc tggaaatcat tattattaac gatggttcaa cggataattc
    19261 tgttgaaata gcaaagcatt acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc
    19321 gaatgctggc gcatcggtgg cgcgtaatcg tgggattgaa gtggcaacgg gcaaatatgt
    19381 cgcttttgtc gatgctgacg atgaagtcta tcccaccatg tacgaaacgc tgatgaccat
    19441 ggcgttagag gacgacctcg acgtggcgca gtgcaacgct gactggtgtt ttcgtgaaac
    19501 gggagaaacc tggaaatcca tccccagcga tcgccttcgc tcaaccggcg tattaaccgg
    19561 cccggactgg ctgcggatgg ggctttcttc gcgccgttgg actcacgttg tctggatggg
    19621 ggtttatcgc cgcgatgtta ttgttaaaaa taacattaaa tttattgccg gattacatca
    19681 tcaggatatt gtctggacaa cagaatttat gtttaatgcg ctgcgtgcgc gatataccga
    19741 gcaatcgtta tataaatatt atctgcataa tacgtcagtg agtcggttgc atagacaagg
    19801 gaacaaaaac cttaattatc aacgtcacta tattaagatt actcgtctgc tagagaaatt
    19861 aaatcgaaat tatgccgaca aaattacgat ttatccggaa tttcatcagc aaataactta
    19921 cgaagcattg cgtgtttgcc atgcggtgcg caaagagccg gatattatta cccgccaacg
    19981 gatgattgcc gagatattta cttccggtat
//
LOCUS       DAJTVK010000076        19740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000076 DAJTVK010000000
VERSION     DAJTVK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.076"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="OO372_002707"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="OO372_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6342160.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="OO372_002708"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="OO372_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6342161.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="OO372_002709"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="OO372_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6342162.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="OO372_002710"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="OO372_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCR6342163.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="OO372_002711"
     CDS             complement(2270..2761)
                     /locus_tag="OO372_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6342164.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="OO372_002712"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="OO372_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6342165.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="OO372_002713"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="OO372_002713"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6342166.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="OO372_002714"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="OO372_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR6342167.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="OO372_002715"
     tRNA            5405..5492
                     /locus_tag="OO372_002715"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="OO372_002716"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="OO372_002716"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="OO372_002717"
     CDS             complement(5764..6450)
                     /locus_tag="OO372_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6342168.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="OO372_002718"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="OO372_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6342169.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="OO372_002719"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="OO372_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6342170.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="OO372_002720"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="OO372_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6342171.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /locus_tag="OO372_002721"
     CDS             complement(10902..11732)
                     /locus_tag="OO372_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6342172.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12398..13906)
                     /gene="putP"
                     /locus_tag="OO372_002722"
     CDS             complement(12398..13906)
                     /gene="putP"
                     /locus_tag="OO372_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR6342173.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14328..18290
                     /gene="putA"
                     /locus_tag="OO372_002723"
     CDS             14328..18290
                     /gene="putA"
                     /locus_tag="OO372_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6342174.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="OO372_002724"
     CDS             complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="OO372_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6342175.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            19256..>19740
                     /locus_tag="OO372_002725"
     CDS             19256..>19740
                     /locus_tag="OO372_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6342176.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         5012 a         5256 c         4806 g         4666 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaga gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcgagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt caacccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgtaact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca
    12241 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgact ggccgccccc
    12301 tgcgcgctcc ctgcaccatt caatttatat ggcgaggcac attaagggag cgcaacccgc
    12361 tgctgaagcc aaacaaaacc gccgcaggct aagtccctta gttttcctgc aaccgtgacg
    12421 gcggagccga atgatagtgc gcatcggcct cggcaaagcg tttttgcatc gccgctgacg
    12481 gcgctttacc cagcaaacta aacactacaa tcccgatact gccgaagatg aagcccggaa
    12541 taatttcgta cagtcccagc cagccgaact gcttccagac gataactgtc agcgcaccga
    12601 tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga ccacatcacc gagaacagca
    12661 ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta gctcactaag cccagcacgc
    12721 ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc caccaccagc accatcacac
    12781 gtcctaccca caccagctct ttctggctgg cctgtttacg cagaaacgct ttgtataaat
    12841 cttcggtaat cgcactggag cacaccagca gctggcaact taaggttgac attaccgccg
    12901 ccagaatcgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
    12961 cgataaacac gcgttcagcg ttctggttta ccgcacctgc taccgccgga tgctcgttaa
    13021 agtaagcgat accaaagaag ccaacagcca ccgcccctgc caggcaaagg atcatccagg
    13081 tcatactgat acgacgcgca tggacaatgc tgtggtgaga atctgccgcc ataaaacgcg
    13141 ccaggatgtg cggctgcccg aagtaaccca gcccccaacc catcaatgag ataatggcaa
    13201 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
    13261 gcgagtcacc aaagccaccg acactgataa tgacgataac cggcgtcagg atcagggcaa
    13321 aaatcatcag gctggcctgt acagtgtcag tccagctcac cgcgaggaaa ccgccaataa
    13381 aggtgtaaag gatcgtcgac gcagctcccg ccaacagagc cgtttcgtaa ctcatgccaa
    13441 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
    13501 acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaaacgcc
    13561 cggtgaaata atccggcaat gttaaggcgt tattattgta ttcggtatgc acacgcaacc
    13621 gcccggccac cagcttccag ttgatccacg cgcctaatgt caggccaatg gcgatccagc
    13681 tttcggaaat cccggaaaga aaaaccgcgc caggcaaccc cattaacagc cagccgctca
    13741 tatccgacgc acccgccgat aatgccgtca cgaatggccc aagactacga ccgcccagaa
    13801 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
    13861 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
    13921 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
    13981 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
    14041 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
    14101 ctctctcaca ttttttgcgg ttgcaccttt caaaaaggtt aactgccgca gagaaaaagt
    14161 ctgagttatt ttttaatcac tgtcatatcg atttctttta ttaacatttc attcattttt
    14221 aagcttgcta cgcaagtcac atttaacatg gttgcacaaa gttgcaacat catggatatt
    14281 tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca
    14341 ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta
    14401 tcgatcgcac accacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg
    14461 aaaacagcga tactctgccg gagctacctg cgctgctttc tggcgcggcc aatgagagcg
    14521 atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa
    14581 tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc ctatcgccgc ccggaaaccg
    14641 aagcggtttc tatgctgctg gaacaagccc gcctgccgca gccagttgct gaacaggcgc
    14701 acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggtcgtg
    14761 caggaatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc
    14821 tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgc gacgcgttaa
    14881 ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt
    14941 ttgttaatgc cgctacctgg gggctgctgt ttactggcaa actggtttcc acccataacg
    15001 aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa ccgctgatcc
    15061 gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca gttcgtcacg ggtgaaacca
    15121 tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg
    15181 atatgctggg cgaagctgca ctgactgccg cagacgcaca ggcgtatatg gtttcctatc
    15241 agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg
    15301 gcatttcaat caaactgtcg gcgctgcatc cgcgctacag ccgcgcccag tatgaccggg
    15361 taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtatgata
    15421 ttggtattaa catcgatgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg
    15481 aaaatctctg tttcgagccg gaactggcag gctggaacgg catcggtttt gttattcagg
    15541 cttatcaaaa acgctgcccg ttggtgatcg attacctggt tgatctcgcc acccgcagcc
    15601 gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgtg
    15661 cgcagatgga cggccttgaa ggctatccgg tttatacccg caaggtgtat accgacgttt
    15721 cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg
    15781 cgacgcacaa cgcccatacg ctggcagcga tttatcaact ggcaggtcag aactactatc
    15841 cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccactgtac gagcaggtca
    15901 ccgggaaagt tgccgacggt aaacttaacc gaccgtgtcg tatttatgcc ccggttggca
    15961 cacatgaaac gctgctggcg tatctggtgc gtcgcctgct ggaaaacggt gctaacacct
    16021 cgtttgttaa ccgtattgcc gacacctctt tgccactgga tgaactggtc gccgatccgg
    16081 tcactgctgt agaaaaactg gcgcaacagg aagggcaaac tggattaccg catccgaaaa
    16141 ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg
    16201 ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct caatagtgca ctgcaaaaat
    16261 ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta
    16321 accctgcgga accgaaagat attgtgggct ttgtgcgtga agccacgcca cgtgaagtag
    16381 aacaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccacg cctccggctg
    16441 aacgcgcggc gattttgcat cgcgctgccg tgctaatgga aagccagatg cagcaactga
    16501 ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgcg
    16561 aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa
    16621 cccaccgtcc attagggcct gtggtgtgta tcagtccgtg gaacttcccg ctggctattt
    16681 tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtgctggca aaaccggcag
    16741 aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt gctggaagcg ggtgtacccc
    16801 ctggcgtggt gcaattgctg ccaggtcagg gggaaaccgt gggcgcgcaa ctgacgggtg
    16861 atgatcgcgt gcgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc
    16921 gcaatatcgc cagccgcctg gacgctcagg gtcgccctat tccgctcatc gctgaaaccg
    16981 gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtcgtggatg
    17041 tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcactgcgc gtgctgtgcc
    17101 tgcaagatga gattgccgac cacacgttga aaatgctgcg cggtgcaatg gccgaatgcc
    17161 ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga
    17221 aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg
    17281 cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga
    17341 cgctgatcga actggatgac tttgccgaat tgcacaaaga ggtctttggt ccggtgctgc
    17401 atgtggtgcg ttacaaccgt aaccagctac cagagctgat cgagcagatt aacgcttccg
    17461 gctatggtct gacgcttggt gtccatacgc gtattgatga aaccatcgcc caggtcactg
    17521 gctcggccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttggtg
    17581 tgcagccgtt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct
    17641 atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg ctcgcgcgtc
    17701 aggatgcaga gtatccggtc gatgcacagt tgaaagccgc attgactcag ccgctaaatg
    17761 cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg
    17821 agctggcgca ggcaggaaca caacgattgc tgccggggcc gacgggtgaa cgcaacacct
    17881 ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag gatgcgctga
    17941 ctcagctcgc cgccgtgctg gcggtgggca gccaggtact gtggccggat gacgcgctgc
    18001 atcgtcagct agtaaaggca ttgccatcgg cagtcagcga acgtattcaa ctggcgaaag
    18061 cggaaaatat aaccgctcaa ccctttgatg cggtgatctt ccacggtgat tcggatcagc
    18121 ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg gtgcagggtt
    18181 ttgcccgtgg cgaaagcaat atccttctgg aacggctgta tatcgaacgt tcgctgagtg
    18241 tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa acaggtagcc
    18301 ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc aataataatc
    18361 cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat cgcgcaacgt cgcgcctgtc
    18421 accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcatgaaa
    18481 atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc
    18541 gcagatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat taacagcggc
    18601 gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc gcgggagact
    18661 tccagcttca gacggatgta ctcttttatc gccgccagcg gagcgaaatc ttcacgaaac
    18721 gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc
    18781 tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcgatc
    18841 tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga
    18901 atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct
    18961 tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc
    19021 ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc
    19081 gtttagtcca ctttttatca gatattttag ataacgtaat attaacatat taataacatt
    19141 aactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat
    19201 gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa
    19261 aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc acgcgccgca
    19321 gtacatgccg acctttgaac tgaataaagc cattgtgcag aaagcggagc attaccattt
    19381 cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga
    19441 tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct cgcgcattca
    19501 gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc
    19561 aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa
    19621 gcccgtgtat gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga
    19681 ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga aaagcgattt
//
LOCUS       DAJTVK010000077        19407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000077 DAJTVK010000000
VERSION     DAJTVK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.077"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..244
                     /locus_tag="OO372_002726"
     CDS             <1..244
                     /locus_tag="OO372_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR6342177.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(295..1062)
                     /locus_tag="OO372_002727"
     CDS             complement(295..1062)
                     /locus_tag="OO372_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6342178.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1258..2166
                     /locus_tag="OO372_002728"
     CDS             1258..2166
                     /locus_tag="OO372_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6342179.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2163..3425
                     /locus_tag="OO372_002729"
     CDS             2163..3425
                     /locus_tag="OO372_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR6342180.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3422..4060
                     /locus_tag="OO372_002730"
     CDS             3422..4060
                     /locus_tag="OO372_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6342181.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4065..4841
                     /locus_tag="OO372_002731"
     CDS             4065..4841
                     /locus_tag="OO372_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6342182.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4930..6294
                     /locus_tag="OO372_002732"
     CDS             4930..6294
                     /locus_tag="OO372_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR6342183.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADTGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6388..7380)
                     /gene="rpoS"
                     /locus_tag="OO372_002733"
     CDS             complement(6388..7380)
                     /gene="rpoS"
                     /locus_tag="OO372_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6342184.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7443..8582)
                     /gene="nlpD"
                     /locus_tag="OO372_002734"
     CDS             complement(7443..8582)
                     /gene="nlpD"
                     /locus_tag="OO372_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6342185.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8722..9348)
                     /gene="pcm"
                     /locus_tag="OO372_002735"
     CDS             complement(8722..9348)
                     /gene="pcm"
                     /locus_tag="OO372_002735"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6342186.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9342..10103)
                     /gene="surE"
                     /locus_tag="OO372_002736"
     CDS             complement(9342..10103)
                     /gene="surE"
                     /locus_tag="OO372_002736"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6342187.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10084..11133)
                     /gene="truD"
                     /locus_tag="OO372_002737"
     CDS             complement(10084..11133)
                     /gene="truD"
                     /locus_tag="OO372_002737"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6342188.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11130..11609)
                     /gene="ispF"
                     /locus_tag="OO372_002738"
     CDS             complement(11130..11609)
                     /gene="ispF"
                     /locus_tag="OO372_002738"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6342189.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11609..12319)
                     /gene="ispD"
                     /locus_tag="OO372_002739"
     CDS             complement(11609..12319)
                     /gene="ispD"
                     /locus_tag="OO372_002739"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6342190.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12338..12649)
                     /gene="ftsB"
                     /locus_tag="OO372_002740"
     CDS             complement(12338..12649)
                     /gene="ftsB"
                     /locus_tag="OO372_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6342191.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12843..13166)
                     /locus_tag="OO372_002741"
     CDS             complement(12843..13166)
                     /locus_tag="OO372_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6342192.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13216..13821)
                     /gene="cysC"
                     /locus_tag="OO372_002742"
     CDS             complement(13216..13821)
                     /gene="cysC"
                     /locus_tag="OO372_002742"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6342193.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13821..15248)
                     /gene="cysN"
                     /locus_tag="OO372_002743"
     CDS             complement(13821..15248)
                     /gene="cysN"
                     /locus_tag="OO372_002743"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6342194.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15250..16158)
                     /gene="cysD"
                     /locus_tag="OO372_002744"
     CDS             complement(15250..16158)
                     /gene="cysD"
                     /locus_tag="OO372_002744"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6342195.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16410..17447
                     /gene="iap"
                     /locus_tag="OO372_002745"
     CDS             16410..17447
                     /gene="iap"
                     /locus_tag="OO372_002745"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6342196.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17715..18008)
                     /gene="cas2"
                     /locus_tag="OO372_002746"
     CDS             complement(17715..18008)
                     /gene="cas2"
                     /locus_tag="OO372_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR6342197.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18005..18928)
                     /gene="cas1e"
                     /locus_tag="OO372_002747"
                     /pseudo
     CDS             complement(18005..18928)
                     /gene="cas1e"
                     /locus_tag="OO372_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18925..>19407)
                     /gene="cas6e"
                     /locus_tag="OO372_002748"
     CDS             complement(18925..>19407)
                     /gene="cas6e"
                     /locus_tag="OO372_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6342198.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4592 a         5232 c         4848 g         4735 t
ORIGIN      
        1 cgcacatgca gattatccgg gtgatgaata tcagtttggt aaagaaatag cagaaagcga
       61 attactctgg cctgttgatc gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa
      121 cggcgctgat tattttatat ttggacatat gatgtttgat aacattcaga cgttcgctaa
      181 ccagatttat attgataccg gcacgccgaa aagcgggcgg ctgtcatttt ataaaataaa
      241 gtagtctcat gcttcttctg tgaagcatga gtaaccaaat gttattgcag gccattattc
      301 atttttcgct accagcaaag agagatcctg cttcgccagc gcgcgactgg cactctccgg
      361 caaaccgtcg tctgtgataa tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc
      421 cacctgacca tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat
      481 cgcccgtttc accgtgactt tatcttccgc tggcgtagaa atcccccgca cactccatga
      541 cgatgcagaa ataaaagcct gatcaatcat caggctgcgc agcatggtcg cagcggcttc
      601 cccgacacag gaacggtttt cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt
      661 actgttgtcg agcaagtagt tcgcaataac gaaatcgttt gtgaccacag tcagtgactc
      721 catgtgaatc agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca gatagataca
      781 acttcccggc tgaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc
      841 cagcgcagtt tttacctgat gagaaggttc atgcgccacg cgtcccggag actggacgcc
      901 tccggacacc agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt
      961 catatgtgac acattcattc tgtccgttag ttcagcaata ctgacaatgc ctttttcagc
     1021 taccatctca aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg
     1081 tcctgctgac attctacgct atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt
     1141 tcacataaaa taacacacaa tgttaattta tgtgaatcag ctcaccatat cgttatcttc
     1201 cagcgcttat attcacaata tcaaacaaaa tatcacttaa attaacaatg agagcagatg
     1261 aaaacgggat ctgagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca
     1321 gcactgtcat gtgtccgcgc aggtctttct acctggggcg cagacctgaa cagcaatgcc
     1381 tgcgctacgt tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc
     1441 gaaaaactgg acgctctgct ggtgctggtg gccaatgcgg cccaggttaa acaggtgctg
     1501 tttggtgaaa caggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc
     1561 actatcgcta gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cgatctggaa
     1621 atgctggatg cgccagtttc tggtggtgca gtaaaagccg ctaacggtga aatgactgtc
     1681 atggcctccg gtagcgatat tgcctttgaa cgactggcac ccgtgctgga agccgttgcc
     1741 ggaaaagttt atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa aattattcac
     1801 cagttgttag cgggcgtaca tattgctgcc ggagccgaag cgatggcact tgcagcccgt
     1861 gcggggatcc cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcctgg
     1921 atgttcgaaa accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc
     1981 gatatttttg ttaaggatct tggtctggtt gccgatacag ccaaagccct gcacttcccg
     2041 ctgccattgg cctcaacagc attgaatatg ttcaccagcg ccagtaacgc gggttacggg
     2101 aaagaagacg atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa
     2161 tcatgatcaa gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt
     2221 ttctggtgga aaacggtcta ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc
     2281 cggaagcaat cgacgcactg gtgatcagcc tgaaaacgcg ctcctgtccg gtggttgaag
     2341 ccacacagca atcgctggcg gctctgagct ggttgcaaca gcaaggttgc aaacagatct
     2401 atttcaaata ctgctctact ttcgacagta cggcgaaagg taatattggc ccggttaccg
     2461 atgccttaat ggatgctctc gacacgccgt ttacggtctt ctctccggcc ctgtcggtca
     2521 acggacgtac ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg
     2581 ggatgcgcca tcacccggta aatcccatga ccgacagcta tcttccccgt ctggttgaag
     2641 cgcaatccac agggcgctgc ggcgtcgttt cggcacatgt tttcgaacaa ggcgtggatg
     2701 ccgttcgtca agagctggct cgcttacagc aagagggcta ccgctacgcg gtgcttgatg
     2761 cgctgaccga acaccatctg gaaattcagg gagaagcctt gcgcgatgcc ccactggtaa
     2821 cgggcggttc tggtctggcg attggcctgg cccggcagtg ggcgcaagaa aacggtaacc
     2881 aggctcgcga agcagggcgt ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct
     2941 ctcaaatgac caaccgccag gtggcacatt accgtcaaat tgcaccagcc cgtgaagttg
     3001 atgtggcacg ctgcctctca accgaaactc tggccgctta tgcacacgaa ctggcagagt
     3061 gggttctggg ccaggaaagt gtacttgctc cactggtttt tgccaccgcc agcactgacg
     3121 cattggcagc aattcaacag caatacggtg cacaaaaagc cagtcaggca gtagaaactc
     3181 tgttttctca actagcggcg cggttagcag cggaaggcgt gacacgcttt attgtcgcag
     3241 gcggtgagac ctccggcgta gtcacacaga gcctgggcat aaaagggttt catattggcc
     3301 caaccatttc ccccggcgtg ccgtgggtaa acgcactgga taagcctgtc tcactcgccc
     3361 ttaaatctgg caacttcggt gatgaagcct ttttttcacg agcccaaaga gagtttttat
     3421 catgagcgat ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc ggattgccag
     3481 ttcattcttt cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc
     3541 tgacgggaat ttactggcga caccgacagg ttcatgcctg ggcaatctcg atccgcagcg
     3601 gctttccaaa gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct cgaaagaggt
     3661 gctctttcat ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca
     3721 cagcacatgg tcgacggcgc tttcctgcct gcaagggctg gacagcagca acgttattcg
     3781 tccgttcaca ccatacgtgg tgatgcggat gggaaatgtc ccgctggtgc cttattaccg
     3841 accgggcgat aaacgcatcg cacaggatct ggcggaactg gcagcagaca atcaggcttt
     3901 tttactggca aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa
     3961 tatggaagag ctggaggaaa cggcaaagct gatttttatt ctcggtgacc gcccgatccg
     4021 ttatctgacc gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag
     4081 ctaatttatc catgatgttc accgaagtgc cttttattga acgcttcgcc gcagcgcgaa
     4141 aagccgggtt cgatgctgtg gaatttctgt ttccctatga ctactccacc ctgcaaatcc
     4201 aaaagcaact ggagcaaaac catctgacac tggcgctgtt caataccgct cccggagata
     4261 ttaatgcggg ggaatgggga ttatccgccc ttcccggacg tgagcacgaa gcacacgcag
     4321 acatcgacct ggcactggaa tatgcattgg cgctcaattg tgaacaagtc catgtgatgg
     4381 caggcgtcgt gcccgctggc gaagacgctg aacggtaccg ggcagtattt atcgataatc
     4441 tccgctacgc tgccgaccgc tttgcgctac acggcaagcg aatattagtt gaagcactca
     4501 gccccgacgt taagcctcat tatctcttct ccagccagta tcaggcactg gctattgttg
     4561 aagaggttgc gcgagataac gtgtttattc aactagatac ttttcatgcg caaaaagtag
     4621 atggcaacct gacgcattta atccgcgact acgctgggaa atatgcgcac gtacaaattg
     4681 ccggactacc tgatcgacat gaaccggacg atggagaaat caactatccg tggctgttcc
     4741 gcctgttcga tgaggtggga tatcagggct ggatcggtta tgaatataaa cctcgtggcc
     4801 tcaccgaaga agggcttggc tggtttgacg cctggcgcta attcgttaaa tccctaatta
     4861 caacgtaccc atacatcccc ccatctacgg atggggtagg ttaactataa ctttcagaca
     4921 gggtttccca tgtccacaat tacattgtta tgcattgcgt tagctggcgt aatcatgctg
     4981 ttgctgctgg tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg
     5041 ttagtcgcac ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg
     5101 atgggcggtg tgcttggttc cgtcactatt attattggtc tgggcgctat gctggggcgt
     5161 atgatcgaac actctggtgg tgcagagtca ctggctaatt atttcagtcg caagttaggt
     5221 gacaaacgaa ctatcgctgc gctgactctg gcagcgttct tcctcggtat tcccgtcttc
     5281 tttgatgtcg gctttattat tcttgctcca atcatttacg gttttgccaa ggttgccaaa
     5341 atatcgccac tcaaatttgg cctgcctgtc gctgggatca tgctaactgt acatgtggcg
     5401 gtaccgccgc atccaggccc tgtcgccgca gcggggttac tccacgcaga caccggctgg
     5461 ctaaccatca tcggtattgc gatttctatt cccgtaggga ttgttggcta ctttgcagcg
     5521 aaaataatca ataagcacca atatgcgatg tcggtggaag tactggaaca gatgcaactg
     5581 gctccggcca gtgaggaagg cgcgacaaaa ttaagcgata aaataaatcc accgggcgtc
     5641 gcgctggtca cctcgctaat tgttattcct atcgcgatta tcatggcggg tacggtttcc
     5701 gcaacactga tgccgccttc gcatcccctg cttggtacgc tacagctgat cggctcacca
     5761 atggtagctc taatgattgc gctggtgctg gcattctggt tattggcttt acgtcgcggc
     5821 tggagcttac aacacaccag cgacattatg ggctcagccc ttcccactgc ggcggtagtg
     5881 attttggtta ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc gggcgtcggc
     5941 aaagcccttg ccaacatgct acaaatgatt gacctgcctc tgttaccagc cgcatttatt
     6001 atttcactgg cgctgcgtgc atcgcagggg tcagcaaccg tagcaatcct gacaaccggc
     6061 gggttactct cagaagcggt gatgggattg aatccgattc agtgcgtatt ggtgacgctg
     6121 gcagcctgct ttggtgggct tggggcgtca catattaatg actcagggtt ctggattgtg
     6181 accaaatatc tggggttgtc ggtagcagac ggtctgaaaa cctggacagt gttaacgacc
     6241 attctcggtt ttaccggatt cttaattacc tggtgcgtat gggcggtaat ttgaccttaa
     6301 taaaaaggtc cgatgagcat cggacctttt attgtgcaca gaaaaggcca gcctcgcttg
     6361 agactggcct ttctgacaga tgcttactta ctcgcggaac agcgcttcga tattcagccc
     6421 ctgcgtttgc aggatttcac gcaaacggcg caggccttca acctgaatct ggcgaacacg
     6481 ttcacgggtg aggccaattt cacgacctac atcttccagt gttgccgctt cgtaccccag
     6541 caaaccgaat cgacgtgcca gtacttcacg ctgtttggcg ttcagctcga acagccattt
     6601 gacgatgctc tgcttcatat cgtcatcttg cgtggtatct tccggaccgt tctctttttc
     6661 atcggccagg atgtccagca acgctttttc ggaatcacca cccagcgggg tgtctaccga
     6721 ggtaatgcgc tcgttaagac gaagcatacg gctgacgtca tcaactggct tatccagttg
     6781 ctctgcgatc tcttccgcac ttggttcatg gtccagctta tgggacaact cacgtgcggt
     6841 tcgcaggtaa acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg
     6901 gttcataatc gcccgttcaa tcgtctggcg aatccaccaa gttgcgtatg ttgagaagcg
     6961 gaaaccacgt tccgggtcaa acttctctac cgcgcggatc agccccaggt tgccctcttc
     7021 gataaggtcc agcaacgcca gaccacgatt gccataacgg cgggcaattt ttaccaccag
     7081 acgcaagtta ctctcgatca tccggcggcg agaggcgaca tctccacgca gtgcgcgacg
     7141 cgcaaaataa acttcttctt cggccgttaa cagtggtgaa taaccaatct caccaaggta
     7201 aagctgagtc gcgtccaaca cacgctgtgt ggctccctgc gataacagtt cctcttcggc
     7261 caaatcgtta tcactgggtt cctcttctac taaggccttt tcgtcaaaaa cctcaactcc
     7321 gttctcatca aattccgcat cttcatttaa atcatgaact ttcagcgtat tctgactcat
     7381 aaggtggctc ctacccgtga tcccttgacg gaacattcaa gcaaaagcct ggttccgccg
     7441 atttatcgct gcggcaaata acgcagcggg tttacggatt tccccttgta acgaatttca
     7501 aaatgcaagc gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc
     7561 ttcacttctt gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa
     7621 tcatcattat gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accggcataa
     7681 acaacgcggc catctgcggt cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc
     7741 cccttgttgc ccccctcaga agcgccaaag gtttcgatca ctttgccctc agtcggccag
     7801 cgccaggtgg agataggcgt actggttgat gtactgctga cagtcggctc ggttgtgctt
     7861 gctgttggta ccgttacagg cgctgtgact gtggtcgcag ttggcttgtt gttcggcaac
     7921 attttgttag cactctgttc acccgaagac tcagaatacg taattgtcgg ttgcgacgca
     7981 acagcaacgg tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc
     8041 tgggtaatgg catttccgcc agtgattggc gtaccggaag cattacccac ctgcaaggtc
     8101 tgaccaacgt tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa
     8161 tcgttgccag taatccaggc gatatagaaa agtgtgtcgc cttttttcac ggtataggta
     8221 ctgccgctat aactgccttt cggaatgttc ccatactgac ggttatagac gatgcgtccg
     8281 ttttccatct gtaccggctg ctgagctact ggctgcactg gctggatttg cggttgttga
     8341 gtagcctgaa tttgtggctg ctgtaccggc tgaatttgcg gttgctgggc tgtagacgtc
     8401 gtccccattt tcggcggcgg cgtaatcaac ataccagaat tagtatttgc aggcgcattg
     8461 ccattaacgg agctgaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat
     8521 agcgaaacca gtgacaaagc agcaatgcgg cgaacggtga attttgggct tcccgcgctc
     8581 atttatcccc caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcaccc
     8641 tactgaacac tggaaaagat gttcacgata cgctgacctg cggcaaaata accaggaaaa
     8701 atccaggtat ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac
     8761 ggcctccacg gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg
     8821 gtgctcctcc cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagcgc
     8881 agttggaatt tccggcggtg ccgccgtaac aatgatagcg tcaaacggcg cacgtgcctg
     8941 ccaaccttgc catccatcgc catgacgggt tgaaacatta tgtaaatcaa gatttttcag
     9001 gcggcgacgt gcctgccact gcaagccttt aatccgttca accgagcaaa catgctggac
     9061 aagatgcgct aggattgccg tttgatatcc cgaaccagtg ccaatttcca gcacccgcga
     9121 ctgcggcgtc agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaattgt
     9181 ctgcccctga cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc
     9241 aacgaatttt tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc
     9301 ttgcgcacgt aattgctcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact
     9361 cccacgctgt ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca
     9421 tgcagcggcg tgatggagac atagccctca tctaccgcag caaaatcggt ccccggaccg
     9481 gcatcacatt taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc
     9541 tgcgggatca cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg
     9601 atttgatcca agggtaaatc cggaacgtta atattaagaa tacgtccggt gcgcagcggc
     9661 tctttacaca gtgcgcgcaa aattgaacag gttaccgccg cggcagtgtc gtaatgttta
     9721 tgcccgtcaa gcgagacggc cagcgccgga aaacctaaat gacggccttc catcgcggcg
     9781 gctaccgtac cggaataaat aacgtcatcc cccagattcg gcccggcgtt aatgccggac
     9841 acaacaatgt ccgggcgcgg acgcatcaga gcattcacgc caagatagac gcaatcggtc
     9901 ggggttccca tttgcacagc aatatcacca ttttcaaagg taaacgtgcg cagggaggat
     9961 tccagtgtca gagaatttga agcgccgctg cggttacgat cgggggcgac cacctgaacg
    10021 tcagcaaact cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca
    10081 tcattactca gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct
    10141 ggttgcaaaa ctacccgccg gaagccagaa acggatctct acggtgacgt catcccacca
    10201 attccagctt aattgttgcg gatacagcaa catcgctctg cgcgcggctt caactttttc
    10261 gcgcaccagt aaagcttgta attcagtttc tgcggcgaca gctgcctttt cgaatgccag
    10321 cgcttcacgc tgagttcccc attcgccact acccggcaat gcggcggtta tcatcagctc
    10381 tttatcgttg acgcgacgct gtaattccgc cagttcttcg gtggttgcga caaaccagct
    10441 accacgtccg gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt
    10501 gaggcgctca gcaacaatct gattaaacaa cgcactgcgg gctgccgaca accaaaaact
    10561 ccgtttattg cgatcgcgca ccggagtatt ggtttgcgcc cagcgctgcg ccccctgcaa
    10621 gttgctaccg ccaatcccaa aacgttgggc accgaagtag ttcggtacac cttttacgca
    10681 aatatcgatc agacgttgtt caacgtcatc gcgattgctc acttcgcgca gaaccagggt
    10741 aaaggcgtta cctttcagcg cgcctaaacg cagcttgcgc ttgtgccgcg catactccag
    10801 cacctggcag ccttccagtt gaaaggcgct cagatcgggc atttccttgc ctggcacgcg
    10861 agcgcataac cactgttccg taacagcatg tttgtctttt tgcccagcga agctgacttc
    10921 acgggcatga attttcagga atttcgccag tgcatccgcc acaaaacggg tattgcagcc
    10981 gtttttgagg attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc
    11041 ttccaccacc acaaagtctt ccggattggc tttcagcagc ccggtgcctt gcggtttacc
    11101 gtggaggtaa gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc
    11161 gcttcacagg caatcccttc cccacgtccg gtaaatccaa gtttttccgt agtagtggct
    11221 ttcacgttaa catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt
    11281 ggaatgtgcg gcaacatctt cggtgcctga gcgataatag tgacatcgac gttgcccagg
    11341 gtataaccct tcgcctgaat acgacgccag gcttcgcgta gcagctcgcg gctgtcggca
    11401 cctttaaatg ccggatcggt atccgggaac agcttgccga tatcccccag cgccgccgcg
    11461 ccaagcaatg catcggtcaa cgcatggagc gccacgtcgc catcagaatg cgccagcaat
    11521 cctttttcgt aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggca
    11581 tgtacgtcaa aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga
    11641 ggtaaaactc ggccagtgcc aaatcttccg ggcgcgtgac tttaatgtta tccgcacggc
    11701 cttcgaccaa ctgaggatgg aatccgcaat attccagcgc cgaggcttcg tcggtaatag
    11761 tcgcgccttc atttagagcg cgcgtcagac agtcatgtaa cagctcacga gggaaaaatt
    11821 gcggcgtcag cgcgtgccat aagccgttgc gatcaacggt atgagcaatg gcatttttgc
    11881 ccggttcggc acgtttcatc gtatcgcgca ctggtgcggc taggatccct cccgtgcggc
    11941 tggtttcgct caacgccaac aatcgcgcga ggtcatcctg atgcagacaa ggacgagcgg
    12001 cgtcatgcac caatacccac tgcgcgtcgc cagcggcttt caaacctgcc agcacggaat
    12061 cggcacgctc atcaccgcca tctacaacgg tgatttgcgg atgattcgcc agaggaagtt
    12121 gtgcaaaacg gctatcgcca ggacttatgg caatgacgac acgtttcacc cggggatgcg
    12181 ccagcagcgc atgcaccgag tgttcaagaa tggtttgatt accgattgag agatattgct
    12241 taggacattc cgtttgcatt cgacggccaa atccggccgc cggaaccacg gcgcaaacat
    12301 ccaaatgagt ggttgccatg ttaattcccg ggctgattta tcgattgttt tgccccgcag
    12361 actgtgcgcg cttcgacgcg tcaggcacca gacgataaaa agtttcgccc ggcctggtca
    12421 tgctgagttc attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa
    12481 tttcggcaaa aagttgatcg tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg
    12541 ccacatcatc attgacgcgg gtatagtcat gtataccgtt cttaccgaac cacagcgaat
    12601 actgtagcca gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg
    12661 aaaaacggca tcatcatccc atgcatccga agacgactct acatcctctg ttggggatac
    12721 cgcgacaacg cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca
    12781 gaacataatc tcattattag ttacggtttg aattatgaac agaggagaca agaaagtaca
    12841 aattagccca gtagccacat aaacagtgcg ccaaacataa tgcctactgt caccagggtg
    12901 aaaacaatac tgtagcgtag ctttccgtcc atcaatgaat gcagcgcaat ccccaccact
    12961 accgcgacgg gcatcagcgc cagaaagaaa ggccaggtgt agataaagaa gaacagcgtg
    13021 ttagagccat aaatcaacat cggcatcgcc agcgcaaata accaggagat aaaaccgacc
    13081 acggcaccag gcagtgacca tgtggtttct tcatcctcag taaggctgtc gttatttgtt
    13141 agtgtaatgt tatggctatt acgcatattt gatcctgtta ctttgacgaa ccgggcatgg
    13201 aaacccggtg gtgtctcagg atctgataat atcgttctgt ctcaacagat ctaataattg
    13261 ctgtaccaaa tttgttacta attgttcacc attgagatga atttctgccg attcaggcgc
    13321 ttcgtaaacg gaatctattc ccgtaaagtt gcgcagttca ccggcacgcg ctttcttata
    13381 taagcctttt ggatcgcggg cttcgcaaat cgccagcggc gtatcgacaa acacttcgat
    13441 aaagcgccct tctcctacgc gttcacgaac catctggcgt tcggcgcggt gtggcgagat
    13501 aaatgcggtc agcaccacca gtccggcttc aaccatcaaa ttcgccactt caccgacgcg
    13561 acggatattc tctttacgat cggcatcgct aaaaccgaga tcgctgcata atccgtggcg
    13621 aacattatcg ccatccagca gatacgtact gacgccgagt ttatgtaacg cctcctccag
    13681 cgccccggcg accgttgatt taccggaccc ggagaggccg gtaaaccaca gcactacacc
    13741 acgatgaccg tggtgtagct cgcgttgttg cacagtgacc ggatggctat gccagacgac
    13801 gttttcgtca tgcagcgcca ttatttatcc cccagcaaat cgcgcgcgcc ccagtgcgga
    13861 aagtggcgac gaaccagagc attcaattcc agttcgaatg cactgaattc agatggcgca
    13921 gcagttgcct ggctaactgg ctcgtgcacc ataccggcac ccacggtcac attgctcagg
    13981 cgatcgataa aaatcagccc acccgtcacc ggattttgtt gataacgatc taacaccagc
    14041 ggctcgtcaa aagtgagatc cacgaggccg atcccattca gtggcaggtt ttcaacttca
    14101 cgctgggtaa ggttattaat atcaacctga tagcgaatgc catcaacacg cgcgcgcgtc
    14161 ttcttaccgg caattttgat gtcgtaactc tgccctggag aaagcggctg ttccgccatc
    14221 cataccacat ccaccgacgc gctctgcacc gccggtaacg cttcgtctgc cgccagcagc
    14281 agatcgccac ggctgatgtc gatctcatcc gtcagcacca gggtgatcgc ttctccggca
    14341 aaggcttctt cgcgatcacc atcaaaagtc acgatccgcg cgacgtttga ttccacacca
    14401 gagggcagca cttttacacg ttgcccgact tccacgcgac cggatgccag cgttccggcg
    14461 tagccacgaa aatcgagatt cgggcgatta acgtactgca ccgggaagcg cattggctga
    14521 gcatccacca ctcgctggat ctccacggtt tccagcactt cgagcagtgt cggaccgctg
    14581 taccacggca tactttcact ttgcgatgcc acgttgtcgc cttccagtgc agagagcggc
    14641 acaaagcgga tatccagatt acccggcagc tgcccggcaa aggtcagata atcttcacga
    14701 atacgggtga acgtctcttc actgtaatcc accagatcca ttttgttgat cgccacgacc
    14761 agatgtttga tccccaacag tgtggagata aaactgtgac gacgggtttg atcgagcacg
    14821 cctttacggg catcgatcag taagatcgcc agttcacatg tcgatgcgcc agtcgccata
    14881 ttgcgagtgt actgctcgtg ccctggggtg tcggcgataa taaatttacg cttctcggta
    14941 gagaaatagc ggtaggccac atcaatagtg atgccctgtt cgcgctcagc ttgcaggccg
    15001 tccaccagca gagccagatc cagcttttcg ccctgggtac cgtgacgctt actgtcgtta
    15061 tgcagcgatg agagctgatc ttcatagatt tggcgagtat cgtgcagcag gcgaccaatc
    15121 agagtgcttt tgccgtcatc gacgctgcca caggtcagaa aacgcagcag gcttttatgt
    15181 tgttgcgcaa tcatccaggc ttcgacgccg ccttcattgg cgatttgttg tgcaagtgcg
    15241 gtgttcatct taaaaatacc cctgacgttt tttcagctcc atagatcccg cctggtcgcg
    15301 gtcaatcacg cggccctgac gttcactggt ggtggaaacc agcatctctt cgatgatttc
    15361 cggcagtgtt tgtgcatttg actccaccgc accggtcagc ggccagcagc ccagcgtacg
    15421 gaaacgcacc atccgttttt taatcacttc gcccggttgc aggtcgatac ggttgtcatc
    15481 aatcatcatc aacataccgt cgcgttccag aaccggacgt tccgcagcga gatacagcgg
    15541 cacaatgtcg atattttcca gccagatgta ttgccagata tcctgctcgg tccagttaga
    15601 gagtgggaag acgcggatgc tttcgccttt gttaatttgc ccgttgtagt tgtgccacag
    15661 ctccgggcgc tgatttttcg gatcccagcg atggaagcgg tcacggaaag agtagattcg
    15721 ctctttagcg cgggatttct cttcgtcacg gcgcgcacca ccgaaggcgg catcaaaacc
    15781 gtatttgttc agcgcctgtt tcaggccttc agttttcata atatcggtat gtttcgcgct
    15841 gccgtgcacg aatggattaa tccccatcgc cacgccttcc gggtttttat gcaccagcag
    15901 ctcgcagccg taggctttcg ccgtacgatc gcggaactca tacatctcgc ggaatttcca
    15961 gccggtatcg acatgcagca acgggaaagg cagcgtacct ggataaaacg ccttgcgcgc
    16021 caaatgcagc atgacgctgg aatctttacc gatagagtag agcatcaccg gatttgagaa
    16081 ttctgccgcc acctcgcgaa taatgtggat gctttccgcc tccagttgcc gcaggtgagt
    16141 aagtcgtatt tgatccataa ccgttccttt gcaataccgc tattttcttg ccatcagatg
    16201 tttcgactat agggagcgta agagaacgaa tgaaattacc aattagaatg agtagttcct
    16261 taacggaata acgatttggc aaagctaata tcaaaaagtg cttaaggcac cggatttctg
    16321 gcgtttagga agatttgaaa ttgttttagc gcagcggaag tttcatacta tggcggtaaa
    16381 aaaatttgca tggtatttaa ggactcacta tgttttccgc attgcgccac cgtaccgctg
    16441 ccctggcgct cggcgtatgc tttattctcc ccgtacacgc ctcgtcacct aaacctggcg
    16501 attttgctaa tactcaggca cgacatattg ctactttctt tccgggacgc atgaccggaa
    16561 ctcctgcaga aatgttatct gccgattata ttcgccaaca gtttcagcaa atgggttatc
    16621 gcagtgatat tcggacattt aatagtcggt atatttatac tgcccgcgat aatcgtaaga
    16681 gctggcataa cgtgacggga agtacggtga ttgccgctca tgaaggcaaa gcgccgcagc
    16741 agatcatcat tatggcgcat ctggatactt acgccccgct gagcgatgct gacgccgatg
    16801 ccaatctcgg cgggctgacg ttacaaggaa tggatgataa cgccgcaggt ttaggtgtca
    16861 tgctggaact ggcagaacgc ctgaaaaata cgcctaccga gtatggtatt cgatttgtgg
    16921 cgaccagcgg cgaagaggaa ggaaaattag gcgctgagaa tttactcaag cggatgagtg
    16981 acaccgaaaa gaaaaatacg ctgctggtga ttaatctcga taacttaatt gttggcgata
    17041 aattgtattt caacagcggt gtaaaaaccc ctgaagcagt aaggaaatta acgcgcgaca
    17101 gggcgctggc aattgcgcgc agtcacggaa tagccgcaac gaccaatccg ggtttgaata
    17161 aaaattatcc gaaaggcact gggtgttgta atgacgcaga aatattcgac aaagcgggca
    17221 ttgctgtact ttcggtggaa gcgactaact ggaatcttgg gaataaggat ggttatcagc
    17281 aacgcgcaaa aacagccgca ttccctgcgg gaaatagctg gcatgacgta agactggata
    17341 atcaacagca tattgataag gcacttcctg gaagaataga acgtcgctgc cgtgacgtta
    17401 tgcggataat gctacctctg gtgaaggagt tggcgaaggc gtcttgatgg gtttgaaaat
    17461 gggagctggg tgttctaccg caggggcggg gaattctaag tgatatccat catcgcatcc
    17521 agtgcgcccg gtttatcccc gctggcgcgg ggaactctta ctgcttggta tgcggaatca
    17581 caccctgaac ggtttatccc cgctggcgcg gggaacacac taagcataca taactgtttt
    17641 taaacaaatt tattccgcat caacaatcta ccaactaaat taaaacattt ccttattttt
    17701 aaagaacgga taacttattg attatcaaca ggaagaaaag aaaccaaacg taacccatcc
    17761 aaatccaccg gaatacgtct gttttctccc caggtctgaa attcaaaacc cgactcgata
    17821 ttggtcgccc aggccatcac cacatttccg caaccagcca gttgggtaat ttgctgccag
    17881 atcatctccc gaatacgttt tgatgtatca ccaacataca caccggcacg cacttccagt
    17941 agccagattg cgagccgtcc acgtaagcgc ggagggacat tttctgtaac aaccacgacc
    18001 atgctcatcc gccgcgcccc cggtgaccac tatcacccag cgtttcaggt tcagggatgg
    18061 caggcggtaa catatccggc gcgggttgtg gtggttcaat ttcacctgca gcaaggactt
    18121 cctcaattaa cggtattaat ttgcccgtta acttagtgct acggaaaata tcgcgacagg
    18181 ctaatctgac ttctttatca ggttctgcgg gttgcctcgc tgctatttca aatgcctttg
    18241 gcacaaccga atcaaattta atgatatcgg ctatgtcata aacaaatgaa agcggtttgc
    18301 cactatggat aaacccaata gcgggcgcat atcccgcggc taatactgcc gcttcagata
    18361 taccgtacag acatgatgta gcagcactga tgcagcgatt cacaacatcg cctttttccc
    18421 agtctttagg atcgtatttg cgaccattcc atttcacacc atattgtttc gccagtaatg
    18481 catacgtctg gcgaacgcga gatccctcaa ttccacgtaa ctgctccact gaacggcgag
    18541 ctggcggtgg ttcacgaaaa cgtaattcat acattttgcg caccaccttc aaccgtagat
    18601 cttccgttaa agccagcttt gcctagtaga gtaatttatc tgcccgcgcc cctccgggtt
    18661 gtccggagga gtaaacgcga acgcccgctt caccgaccca gaccagcagt gttcccaccg
    18721 tggcggccag atggactgcc gcgtgggaaa ctcttgttcc cggttcgagc ataatgcagg
    18781 cgaccgatcc cactggaata tgcgtgcgga tcccggtttt gtcgatcagc acgaaagcgc
    18841 cgtccagtac gtcgatttga ccgtactgga ggaagatcat ggaagtgcga tcttttaatg
    18901 ggatcgggct cagcggtaca aacgtcacgc ctctgctccg ggtttgatca gcatcagacc
    18961 acaaccgaac gcccggcttt tgccataccc ctgactgaga cgttgcagaa ataacccagg
    19021 agcggtgacc gtaagcatcc ccgtataatc cacgctactg aactggatca gttgccggga
    19081 gttttcccgc cgcagttgct gttgtcggta ggcatcaacc gaagtatcca gcagtgtaaa
    19141 accgcttctc tccccctgcg ctgccagcca gtccagcgca gcctgttgtt gatgcaacca
    19201 gacatcactt ccttccacct gccccctcac ctgccgtttc gcctccatca gcaagtcgtg
    19261 gcgtttgccc gctttacaaa ttgttggatt cgcccgcagg ttgaaacaaa gggattgtcc
    19321 ggtacgcaac tctggcgcaa atggtcggca ttcgatggtg aaaatatcac tttccgccgg
    19381 gcgttcctgc gacaggacaa aaaagcg
//
LOCUS       DAJTVK010000078        18844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000078 DAJTVK010000000
VERSION     DAJTVK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.078"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="OO372_002749"
     CDS             complement(254..2125)
                     /gene="ppiD"
                     /locus_tag="OO372_002749"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6342199.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2317..2589)
                     /gene="hupB"
                     /locus_tag="OO372_002750"
     CDS             complement(2317..2589)
                     /gene="hupB"
                     /locus_tag="OO372_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6342200.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2798..5152)
                     /gene="lon"
                     /locus_tag="OO372_002751"
     CDS             complement(2798..5152)
                     /gene="lon"
                     /locus_tag="OO372_002751"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6342201.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="OO372_002752"
     CDS             complement(5340..6614)
                     /gene="clpX"
                     /locus_tag="OO372_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6342202.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="OO372_002753"
     CDS             complement(6740..7363)
                     /gene="clpP"
                     /locus_tag="OO372_002753"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6342203.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7609..8907)
                     /gene="tig"
                     /locus_tag="OO372_002754"
     CDS             complement(7609..8907)
                     /gene="tig"
                     /locus_tag="OO372_002754"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6342204.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="OO372_002755"
     CDS             complement(9251..9568)
                     /gene="bolA"
                     /locus_tag="OO372_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6342205.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9873..10451
                     /locus_tag="OO372_002756"
     CDS             9873..10451
                     /locus_tag="OO372_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6342206.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10495..11970
                     /gene="ampG"
                     /locus_tag="OO372_002757"
     CDS             10495..11970
                     /gene="ampG"
                     /locus_tag="OO372_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6342207.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12431..13378
                     /gene="cyoA"
                     /locus_tag="OO372_002758"
     CDS             12431..13378
                     /gene="cyoA"
                     /locus_tag="OO372_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR6342208.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13400..15391
                     /gene="cyoB"
                     /locus_tag="OO372_002759"
     CDS             13400..15391
                     /gene="cyoB"
                     /locus_tag="OO372_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR6342209.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15381..15995
                     /locus_tag="OO372_002760"
     CDS             15381..15995
                     /locus_tag="OO372_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6342210.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15995..16324
                     /locus_tag="OO372_002761"
     CDS             15995..16324
                     /locus_tag="OO372_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6342211.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16336..17226
                     /gene="cyoE"
                     /locus_tag="OO372_002762"
     CDS             16336..17226
                     /gene="cyoE"
                     /locus_tag="OO372_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6342212.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17411..17722
                     /locus_tag="OO372_002763"
     CDS             17411..17722
                     /locus_tag="OO372_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6342213.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17706..18248
                     /locus_tag="OO372_002764"
     CDS             17706..18248
                     /locus_tag="OO372_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6342214.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18304..>18844)
                     /locus_tag="OO372_002765"
     CDS             complement(18304..>18844)
                     /locus_tag="OO372_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6342215.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4955 c         4580 g         5093 t
ORIGIN      
        1 ccgccgtcgg tttcgccaca gaagctgccg ccagcgcgct atgagacatt ccggcacagg
       61 ccagggacag ggtaatgagc agtgctttaa ttccgtgttt catactgttt tctccttgtt
      121 tgttaacagt ggaatcacct taacgggagt gaaaacagta aacaaatggc aatgttcaga
      181 aatggaaaag gccgcgaaag cgaccttttc ttgttacagc tgcaacgcat ttttttgcga
      241 ggcggcttca ggattattgc tgttccagcg catcgccaat tttgattttc gcctctttac
      301 gcaggttact catcagagct tcaaagacga tttgtgcgtt gttctgggtg ataccctgca
      361 ccatcgcttt tttctgatct tccggcattg aaccttgttt cacttcatcc agcgccagca
      421 gaaccacatt accttgcata tcggtcgcca taccgtagct cggtttgtct ttcgctggca
      481 gtggcagtgc aaacgccgcc tggctaatcg ggtcacgacc ggaacggctt aaggttttcg
      541 gctcgccaaa tttcagaccg gcagcctgca tagcttccgc ccctttgccg gctttcaaat
      601 caaccagcag tttctcagca tccactttcg cctgttgttc agctttgttg tgctgaacca
      661 gtgccttaac ttgttcctga acatctgcca acggtttcac cgcttccggt ttgtgctcgc
      721 tgatgcgcag cacgaatgcg cggtcgccgt ctacggtgat gatgtcagag ttgatgcccg
      781 gcgcgccgtt ttcacctacc agaccgccgt taaagatagc gtcggcaacc ggcttgaagt
      841 tcaactcttc cggcaggtta tctttgctga accaacccgt ctgagtggct ttaacgccgg
      901 cagcttgctc tgcaccggcc agagactcgg tgtcgttgct tgccgcatcg ctcactttct
      961 gctgcagcgc atagtacgca tcgagggctt tttcgtgttt cactttcgcc gcaatgtcgt
     1021 cacgtacttc gtctaacgat ttcactttcg ctggctgaat gtcgtccaga cgtacaatca
     1081 ggaaaccgac cgaagatttg atgacaccag acagttggcc tttttctttc agaccagcat
     1141 ttttcagttc gtcggggata gtggcatctt ctaaccaacc catatcgccg ccgttacggg
     1201 cagagataat atcggcagat ttttctttgg ctaatgcagc aaaatcaccg cctttattca
     1261 gctcatcaag taccgctttc gcttcatctt cagttttggt ctggatgatg ctgtagcggg
     1321 tacgctgcgg ctgggtgaat tgatcctgat gctgatcgta gtagctctgg atatccgcat
     1381 cgctaaccgg ttgctgcatc gttgcggcat ccagcttgat gtaactcacg cggaattgtt
     1441 ccggcgtcat gaaattgttt ttgttttgtt cgtagtagct ggcaatctcc tgttcggtca
     1501 caggctgctt cgccgccagc gcgttaacat cgatagtcgc ctcacgcacc acgcgttgtt
     1561 gcgcgaccag tgccgccagc tcgtcggttt cacctttcag cataaaatcg gtaccggcaa
     1621 caccgttaat cagctgttgg gtagtgagct ggttacgcag cgcctgggcg tactgatcgg
     1681 cggtcatccc catctggttg aggataccgt tatagcggct gttatcaaat ttgccgtcaa
     1741 cctggaaggc tggggtcgcg aaaatcgcct gtttaacctg ctcatcactg atacccagtt
     1801 tcagctcacg agcgtactga tccaacagcg cctcgtcgat cagacgattc agcacctgtt
     1861 gacgcagggt tttcatatag ccttcgttcg ctgccagctc ggagtattga tcgcccagct
     1921 gttgctgcat gcgattacgc tcgctgttaa aggcgttctc gaactgccca cggctgattt
     1981 cctggtcatt cacttttgcg gcgtagttat tgcctccgcc aatcaggtaa ccactcacgc
     2041 cggtcaatat gaacgacacg ataatgatac cgaaaataat cttgagcacg agactgtttg
     2101 cagccgtgcg taagctgtcc atcatggtgt aacaacactc cgctgtagat gactgtgtac
     2161 ctcacgcaac gtatcgatgc gcgcaaaggc ctattgtgac aagaaacggg ggcaattgtc
     2221 agcccacaag acacagaaag tcaccgaata caaataaaaa aggcacatca gtagatgcgc
     2281 ccttgaactt cgtcacatcc ccactgggga caacgcttag tttaccgcgt ctttcagtgc
     2341 tttacctgca cggaagctcg gtactttagc ggcagcgatg gtgatctctt taccggtctg
     2401 cgggttgcgg ccagtacggg cagcacgctc tttaacggca aaagtaccaa aacctaccag
     2461 tgctacatca tccccttctt tcagagattc agttacggaa gcaataatag catctaacgc
     2521 acggccagcc gcagctttag agatatcagc ccctgcagca atcttgtcga tcaattgaga
     2581 tttattcact cttctcttcc tctttataat ttatatcgca cttgaatcct tcaaggtacg
     2641 aacgcgcagc agttatatca ggccagccat ccccttacaa caccccttaa taagggcaag
     2701 cccgatccgc catctaactt agcgagacaa aaaaaggctg gcaagcccga attagcctgc
     2761 cagccctgtt tttattagtg cattttgcgc gaggtcacta ttttgcagtc acaacctgca
     2821 tgccagacgg ttcattttgc agcgccagag tcagaacttc ctcaatgcgc ttcacaggat
     2881 gaatgtccag atcggcaatt acgttgtcag gaatctcttc cagatcgcgt ttattttcga
     2941 acggaattag cactgtttta atcccgccgc gatgcgctgc caggagtttt tctttcaaac
     3001 caccgatcgg cagtacctga ccacgcagag tgatctcacc ggtcattgcc acatcggcac
     3061 gaaccgggtt accggtcagg caagaaacca gcgcggtgca catagcaata ccggcactcg
     3121 gaccatcttt cggcgtcgca ccttccggta cgtggacgtg gatgtcacgt ttttcgtaaa
     3181 aatcagggtt gatccccagt ttttccgcac gcgcacgaac caccgttaac gccgcctgaa
     3241 tagactcctg catcacttcg ccgagcgaac cggtataggt cagtttgcct ttacccggaa
     3301 cacatgcggt ttcaatggtc agcaagtcac cgcccacttc cgtccacgcc agaccggtta
     3361 cctgaccgac acggttttcg ttatcagcgc gaccatagtc gaaacgctga acaccgaggt
     3421 agtcatgcag gttatcgccg ttaatttcga tatgttttaa tgacttatcg agcagtaact
     3481 gcttaaccgc tttgcggcac agtttggaga tttcacgctc cagaccacgc acgcccgcct
     3541 cacgggtgta gtaacgaata atgccgataa tggcgctatc gtcgacggtc agctcacctt
     3601 ttttcagtgc attacgttca atctgcttcg gcagcaggtg acgtttggcg atgttcagtt
     3661 tttcatcttc ggtataaccg gagaggcgaa tcacttccat acgatccagc agcggtgccg
     3721 gaatgttcat ggagttcgac gtcgcgacaa acatcacgtc gctgagatcg taatccactt
     3781 ccaggtagtg gtcgctgaac gctacgttct gctctggatc cagcacttca agcagtgcag
     3841 aggccggatc gcctcgcatg tcagaagaca ttttgtcgat ctcatcgagc aggaacagcg
     3901 ggtttttcac gcccactttc gccattttct ggatcagttt acccggcata gaaccgatgt
     3961 aagtacggcg gtgaccacgg atttccgctt catcacgcac gccgcccagc gccatacgga
     4021 catatttacg cccggtggct ttggcaatgg actgaccaag agaggtttta cctacccccg
     4081 gcggccctac caggcagagg atcggtccct tgattttgtt gacacggctt tgaaccgcaa
     4141 ggtactcaag gatgcgatct ttcacgcgct ccagaccata atggtcggta tcaaggattt
     4201 cctgcgcctg acgcaggtct tttttgacct tgctgcgcgc attccacggt acctgtacca
     4261 tccagtcgat ataaccacgc actacggtcg cttctgccga catcggagac atcattttca
     4321 gcttctgcaa ctctgcttcc gctttctctt ttgcctcttt cggcatcttc gccgcgtcga
     4381 ttttgcgctt cagggcttcg ttttcgtccg gcgcgtcgtc catttcaccg agttctttct
     4441 gaatagcttt catttgctcg ttcagatagt actcacgctg ggatttctcc atctgctttt
     4501 taacgcggtt gcgaatgcgt ttctcaacct gcagcagatc gatttccgat tccatcattg
     4561 ccatcagata ttccagacgt tcgttaacgt cggacatctc cagaacggac tgtttgtcag
     4621 ccagtttcag cggcatatgt gcagcaatgg tatccgccag acgcgccgga tcgtcgatgc
     4681 tattcagcga cgtcagcact tctggtggga tttttttgtt cagcttgatg tagccttcga
     4741 actggctgat tgcagtacgc accagcactt cctgttcccg ctcatcaatg gtcggcgact
     4801 ccagatactc cgccttcgca gaaaagtgtt cgccattgtc agagagcgca gaaatacgcg
     4861 cgcgctgtaa cccctcgacc agcactttga cggtgccgtc aggcagtttc agcatctgca
     4921 atatagaggc cacggtcccg acggtgaaaa gatcgtttac acccggctca tccgttgaag
     4981 cttctttctg cgcgaccagc ataatttttt tatcatggtc catcgccgct tccagacaac
     5041 ggatagattt ttcccgcccg acaaataagg ggatgaccat gtgcggataa accaccacat
     5101 cgcgcagcgg caatacgggg atttcaatgc gttcagaacg ctcaggattc atagagctct
     5161 ctcttagttt aatttccgcc aggtaatcag atgacacgac tgtgcttcac gccatctatt
     5221 aacatgtacg tcagtatatg gggatgtttc ccccacattc aacgccgaga atagaggaaa
     5281 aattaaaggg gagataaaat cccccctttt tggttaacta attgtatggg aatggttaat
     5341 tattcaccag atgcctgttg cgcttccggc ttaccataaa tcagcaacgg tttgctttga
     5401 ccatcaatta ccgactcgtc gataaccact ttttcgacgt cttccatgga cggcagatcg
     5461 tacatggtat cgagcagtgc ggcttctacg atggaacgca ggccacgggc accggtttta
     5521 cgcgccatcg ctttcttagc gatagcatcc agcgcctcgt cacggaattc cagatccacg
     5581 ccttccagat taaacagcgc ctgatactgc ttggtcaggg cgtttttcgg ctctttgagg
     5641 atctgaatca gagcttcttc gctcagttca ttcaacgttg ctacaaccgg cagacgacca
     5701 atgaactcag ggataagacc aaacttgatc agatcttccg gttcaacctg cgccagcagc
     5761 tcgccttcgc ttgctttgtc ggacttcgct tttaccgtcg cgccaaaacc aatgccggag
     5821 ccggtttcta cacggtggga aatcacttta tccagaccgg caaacgcacc gccacaaata
     5881 aacaggatct tagaggtatc aacctgcaag aattcctgct gcggatgttt acgcccacct
     5941 tgcggtggaa cagcagctac cgtaccttcg atcagtttca acagtgcctg ctgtacgcct
     6001 tcaccggaaa cgtctcgggt aatggacggg ttgtctgact tacgagaaat cttgtcgatt
     6061 tcatcgatgt agacaatccc gcgctgcgct ttctgtacgt cgtaatcgca cttttgcaac
     6121 agcttctgaa tgatgttttc aacgtcttcg cccacataac cggcttcggt cagcgtggtt
     6181 gcgtcggcca tggtgaacgg gacgtccagc aggcgcgcca gcgtttcagc cagcagcgtt
     6241 ttaccggaac cggtcggacc gatcagcaga atgttacttt tgcccaactc gacaccattg
     6301 ctggtatcgc cgttgcgcag acgtttgtaa tggttgtata ccgcgaccgc cagcactttt
     6361 ttcgcctgtt cctggccgat aacgtaatcg tccaggtggt tgcgaatttc atgcggcgtc
     6421 ggtagcgcac tgcgttcacg atgcggtgca acttctttaa tctcttcgcg aatgatgtcg
     6481 ttacataaat caacacattc gtcgcagata tacacggatg gaccggcaat cagcttgcgc
     6541 acttcatgct ggcttttgcc gcaaaaagag caatacagca atttgcctga gccatctttg
     6601 cgtttatctg tcatgggtca aaacctcttc tttgttcttt gtgccgcaca cgacgacgca
     6661 aatgccattc tcaggcgcaa gccgcttaca gcgttgtgcc gccctggata agtatagcgg
     6721 cacagttgcg cctctggcat caattacgat gggtcagaat cgaatcgacc agaccgtatt
     6781 ccaccgcttc aggggcggaa aggaagcgat cgcgctcggt atcacgttca atctgttcta
     6841 atgattgacc cgtatgaagc gccataagtt cattcatgcg ccctttaact ttcagaattt
     6901 cacgggcatg aatttcgata tcggtcgcct ggccctggta gccgcccaac ggttggtgaa
     6961 tcatcacgcg cgaattcggc aggcaaaaac gtttaccttt tgcccctgcg gtcagcaaga
     7021 aagcgcccat cgaggccgcc tggcccatac agatggtgct gacatcaggc ttgataaact
     7081 gcatggtgtc atagatagac atcccggcag tgatcacccc gcctggggag ttaatgtaca
     7141 gatagatatc tttttctggg ttttccgctt ctaggaacag catctgcgcc acaatcaggt
     7201 tcgccatgtg gtcttcaacc tggccagtca gaaaaatgac gcgttcctta agtagacgag
     7261 aatagatatc aaaagagcgc tcaccgcgtg aggtctgttc aatgaccatc ggcaccagcg
     7321 ccatatgggg tgcaaagtta tctcgttcgc cgctgtatga catttccgtc tcctggataa
     7381 aattgaaaaa acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg
     7441 tgttatgggt acggcatcac catatttcaa gcataacaat cttttgttat tacgctaaca
     7501 ccgaaagagg cttttcgcac ggttccatgc aaaattcatg acaaaaaaaa gcccaccacc
     7561 tggcggtgac gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg
     7621 gttcatcagc tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc
     7681 ttcaacagcc tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt
     7741 gctgtagaac tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag
     7801 gcctttcacg cgctcttcgt cagctttcag ttcgttggtg cggataactt cgcccagcag
     7861 caggccaaca actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag
     7921 agcttgtttt tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc
     7981 gctgtcgatc agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc
     8041 ctgagactta acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac
     8101 ttcagcgcgc agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc
     8161 agtcagttcc ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc
     8221 tttacctttc agattttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc
     8281 ttcgccagct ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc
     8341 catcgccagt acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc
     8401 ggtgaagtcg atggttacgc ggtcttctgc ttcaacggcg ccgtcttttt ctttccaggt
     8461 cgcctgctgt ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac
     8521 gatcggtttt tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc
     8581 aaactctaca gagtaggtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg
     8641 tgcgccagcc ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag
     8701 gtcacccaga acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg
     8761 cactttgcct ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct
     8821 tttaacagcg gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc
     8881 ttgagtggtt tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca
     8941 actcttaccc cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa
     9001 tcacattccc tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc
     9061 ggcatcactt ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt
     9121 cccgggcaat tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg
     9181 gcccgcaggc cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc
     9241 aaatgcgttt ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt
     9301 cctgcaaccc ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag
     9361 tagagagttc ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac
     9421 ccgtaaaacg atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat
     9481 tgtgacgata gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt
     9541 tttcttctat ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca
     9601 tccatccctt taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt
     9661 taggtttcgt cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac
     9721 acaggcctcg catcagcgtt tcttttacca ggggtgatta tttcgccaac atgatgaatt
     9781 tacatgcctt acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc
     9841 aaacacgctt aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag
     9901 ttgctctgtt tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga
     9961 cgattacact gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg
    10021 ccgatcagcg taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc
    10081 tgaccgcctc ccgagatctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg
    10141 cgcgtggtta catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac
    10201 tgtatgctga cgtgtcccag ggcaacgtgc gctacaacat cgcgaccaaa gcggatatcg
    10261 ccatcatcgc taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca
    10321 acgttgaagg tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc
    10381 tgaccgatac catcgctgat atgtctcagg acaccagcat ccacgaattc atcaagcaga
    10441 acgcgcgtta atttctgccc tctggcccgg tgcaaaccgg gcctgtagac gcccatgtcc
    10501 agtcaatatt tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt
    10561 gcttccgggc taccgctcgc cctgacatcc ggtaccttac aggcctggat gacggtcgag
    10621 aatattgatc tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa
    10681 ttcctctggt caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt
    10741 tggctgctcg ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa
    10801 cctggcaccc aactccgctg gatggcggca ctggcggtgg tgatcgcttt ttgctcggcc
    10861 tcccaggata ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt
    10921 gcgggcgcgg caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggctta
    10981 gccctgtggc tggcagataa atggctgggc tggcagggca tgtactggtt gatggcggcg
    11041 ctgttgatcc cttgtattat cgcgacgttg cttgcaccag aaccgaccga caccattcct
    11101 gtgccaaaaa cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat
    11161 aatgcctggc ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg
    11221 agcctgacaa ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg
    11281 gttaacaaaa cgcttggctt actggcgacc attgtgggcg cattgtacgg tgggattttg
    11341 atgcagcgcc tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct
    11401 aacgctggtt actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc
    11461 gtctttttcg aaaacctctg tggcgggatg ggcacatcag cctttgtcgc actgttaatg
    11521 acgctgtgta ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct
    11581 gtaggacgag tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg
    11641 acattctatc tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc
    11701 cgccagacgc ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg
    11761 acaggttatg cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg
    11821 ttactgctgt tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg
    11881 ctggaagtcg gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat
    11941 ctggctctac gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca
    12001 ctcgccgtaa ttattacggc taaataaaca tcagtatcgc ttaataatat ttgttctttt
    12061 gtgcggctta gcgtttggaa attattgacg caatttatga attctatttt ttcttacacg
    12121 attcagctaa tgagtcttta ttttcccccc acccaattgt caccataatg ataattattt
    12181 gttaaacaat tgttttattc aagattggtt ataccaattg cccgtccaaa ctccttctgc
    12241 actccccttt cgttataacg cccttttgca acagcttctt aaaatcaact tgatatgttt
    12301 tgcaacatat gtaacctgac agccaaatcc aagtaacagg aatttaatca tgtttacagt
    12361 aatgtaacct ccccgtaaaa tgcccacaca ctttaaacgc caccagatcc cgtggaattg
    12421 aggtcgttaa atgagactca ggaaatacaa taaaagtttg ggatggttgt cattatttgc
    12481 aggcactgta ttgctcagtg gctgtaattc tgcgctgtta gatcccaaag gacagattgg
    12541 tctggagcaa cgttcactga tactgacggc atttggcctg atgttgattg tcgttattcc
    12601 cgcaatcttg atggctgttg gtttcgcctg gaagtatcgt gcgagcaata aagatgctaa
    12661 gtacagcccg aactggtcac actccaataa agtggaagct gtggtctgga cggtacctat
    12721 cttaatcatc atcttccttg cggtactgac ctggaaaacc actcacgctc ttgagcctag
    12781 caagccgctg gcacacgacg agaagcccat taccatcgaa gtggtttcca tggactggaa
    12841 atggttcttc atctacccgg aacagggcat tgcaaccgtg aatgaaatcg ctttcccggc
    12901 gaacactccg gtgtacttca aagtgacctc caactccgtg atgaactcct tcttcattcc
    12961 gcgtctgggt agccagattt atgccatggc cggtatgcag actcgcctgc atctgatcgc
    13021 caacgaaccc ggtacttatg acggtatctc cgccagctac agcgggccgg gcttctcagg
    13081 catgaagttc aaagctattg caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa
    13141 agcgaaacag tcgccgaaca ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc
    13201 tagcgaatac aaccaggtgg aatatttctc caacgtgaaa ccagacttgt ttgccgatgt
    13261 gattaacaag tttatggctc acggtaagag catggacatg acccagccag aaggtgagca
    13321 cagtgcacac gaaggtatgg aaggcatgga catgagccac gcggaatccg cccattaaag
    13381 gggttgagga agaataaaga tgttcggaaa attatcactt gatgcagtcc cgttccatga
    13441 acctatcgtc atggttacga tcgctggcat tattttggga ggtctggcgc tcgttggcct
    13501 gatcacttac ttcggtaagt ggacctacct gtggaaagag tggctgacct ccgtcgacca
    13561 taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg atgttgctgc gtggttttgc
    13621 tgacgccatt atgatgcgta gccagcaggc tcttgcctcg gcgggcgaag cgggcttcct
    13681 gccacctcac cactacgatc agatcttcac cgcgcacggc gtgattatga tcttcttcgt
    13741 ggcgatgcct ttcgttatcg gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg
    13801 tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg tttactgttg ttggtgtgat
    13861 tctggttaac gtttctctcg gcgtgggcga atttgcgcag accggctggc tggcctatcc
    13921 accgctatcg ggaatagagt acagtccggg agtcggtgtc gattactgga tatggagtct
    13981 ccagctatcc ggtataggta cgacgcttac cggtatcaac ttcttcgtta ccattctgaa
    14041 gatgcgcgca ccgggcatga ccatgttcaa gatgccagta tttacctggg catcactgtg
    14101 cgcgaacgta ctgattattg cttccttccc aattctgacg gttaccgtcg cgttgttgac
    14161 cctggatcgc tatctgggca cccatttctt taccaacgat atgggtggca acatgatgat
    14221 gtacatcaac ctgatttggg cctggggcca cccggaagtt tacatcctga tcctgcctgt
    14281 tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac
    14341 ctcgctggta tgggcaaccg tctgtatcac cgtgctgtcg ttcatcgttt ggctgcacca
    14401 cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc tttggtatca ccacaatgat
    14461 tatcgccatc ccgaccgggg tgaagatctt caactggctg ttcaccatgt atcagggccg
    14521 catcgtgttc cattctgcga tgctgtggac catcggtttt atcgtcactt tctcggtggg
    14581 cgggatgact ggcgtgctgc tggcagtacc gggcgcggac ttcgttctgc ataacagcct
    14641 gttcctgatt gcgcacttcc ataacgttat catcggcggc gtggtcttcg gctgcttcgc
    14701 agggatgacc tactggtggc ctaaagcgtt cggtttcaaa ctgaatgaaa cctggggtaa
    14761 acgcgcgttc tggttctgga tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct
    14821 gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt gacccgcagt tccacaccat
    14881 gctgatgatt gcagctagcg gtgcagtact gattgcgctg ggtattctct gcctcgttat
    14941 tcagatgtac gtttctattc gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg
    15001 gggtggccgt acgctggagt gggcaacctc ttccccgcct ccgttctata actttgccgt
    15061 tgtgccgcac gttcacgagc gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta
    15121 taaaaagcct gaccactatg aagaaattca tatgccgaaa aacagcggtg caggtatcgt
    15181 cattgcagct ttctccacca tcttcggttt cgccatgatc tggcatatct ggtggctggc
    15241 gattgttggc ttcgcaggca tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt
    15301 ggattactac gtgccggtgg cagaaatcga aaaactggaa aaccagcatt tcgatgagat
    15361 tactaaggca gggctgaaaa atggcaactg atactttgac gcacgcgact gcccacgcgc
    15421 acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt cggattttgg atctacctga
    15481 tgagcgactg cattctgttc tcaatcttgt ttgctaccta tgccgttctg gtgaacggca
    15541 ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc gttcgttctg gttgaaactt
    15601 tcttgctgtt gttcagctcc atcacctacg gcatggcggc tatcgccatg tacaaaaaca
    15661 acaaaagcca ggttatctcc tggctggcgt tgacctggtt gtttggtgcc ggatttatcg
    15721 ggatggaaat ctacgaattc catcacctga ttgttaacgg catgggtccg gatcgcagcg
    15781 gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg tctgcacgtc acttccggtc
    15841 ttatctggat ggcggtgctg atggtacaaa tcgctcgtcg cggcctgacc agcactaacc
    15901 gtacccgcat catgtgcctg agcctgttct ggcacttcct ggatgtggtt tggatctgtg
    15961 tgttcactgt tgtttatctg atgggggcga tgtaatgagt cattctaccg atcacagcgg
    16021 cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt atcctgtcga tcattctgac
    16081 ggtgattccg ttctggatgg tgatgacagg agctgcctct ccggccgtaa ttctgggaac
    16141 aatcctggca atggcagtgg tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa
    16201 taccaaatca gatgaaggct ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc
    16261 tatcctggtt gtaggctcca tctggattat gtggaacctc aactacaaca tgatgatgca
    16321 ctaagagcgg cggttatgat gtttaagcaa tacctgcaag taacgaaacc aggcatcatc
    16381 tttggcaacc tgatctcggt gattggggga ttcctgctgg cctcaaaggg cagcattgat
    16441 tatcccctgt ttatctacac gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg
    16501 tttaacaact acatcgacag ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg
    16561 ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt acgccacctt gctaggcttt
    16621 gctggcttta tgctgctgtg gtttggcgcg aatccgctgg cctgctggct gggggtgatg
    16681 ggctttgtgg tttatgtcgg cgtttatagc ctgtacatga aacgccactc ggtctacggc
    16741 acattgattg gttcgctctc cggcgctgcg ccgccggtga ttggctactg tgcagtaacc
    16801 ggcgagttcg atagcggcgc agcgatcctg ctggctatct tcagcctgtg gcagatgcct
    16861 cactcctatg ccatcgccat tttccgcttt aaggattatc aggcggcaaa cattccggta
    16921 ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc
    16981 tttgccgttg ccacgctgat gctctctctt ggcggttacg ctgggtataa atatctggtg
    17041 gtcgccgcgg cggtgagcgt ctggtggtta ggtatggccc tgcgcggtta taaagttgct
    17101 gatgacagga tctgggcgcg caagctgttc ggcttctcta tcatcgccat cacagccctc
    17161 tcggtgatga tgtccgttga ttttatggtg ccggactcgc atacgctgct ggctgctgtt
    17221 tggtaacaca acctctctat taaaaaggtg ctacggcacc ttttttattg tctgagatca
    17281 gagatacggc taataaccgt atgtcttgac agagtgagca gatgaacata aattaatcat
    17341 acggttgata gccgtattat gtatgagatc gactccctac gatttcattc agttaatcaa
    17401 agaggtcata atgcctggaa aaacagccac tctacccgat gtcgataaaa cgctgaaaaa
    17461 tgcgcgggtc gaactcaaaa ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca
    17521 agccgttggg gtagatttaa gcgcttttat tttgagcgct gctatggaac gcgccgaaag
    17581 tgtgcttgat aaccaacgtc gccgtgagct ttcgaatcaa agctgggaac tgatgaacca
    17641 actcatcgct gaacctgctc aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa
    17701 cagcgatggt cgacaagcat gaagagatta ctctgcccat agtcctctcc tgtaattatc
    17761 agtctgatat tacttatcct gggcaaaaac agtttgattg cggtaaccct gttatcgata
    17821 aatttgtacg cgcatcgcta aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac
    17881 ttattgacag acaaagtggt gaactgatcg gcatctgtac ttttacggca tattcgctgg
    17941 aaaaacaacg cgtttctggc gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca
    18001 gattagtcat gttgggggta gcacggaagt atcaaaagcg gggctttggt caggacctac
    18061 tatgtgattt ttttgaacat gtaaaaataa ttcaccaggc attaccaatt aaaggggttt
    18121 atcttgatgc tgaccctgcc gccattaatt tttatgctcg tctcggcttt gttcagcttt
    18181 cagcgacacc aaatgctttt ggtgctgtac ctatgttttt ggcgattcag catattctcg
    18241 cggcttagat tattatcgcc acccgccagg gcaagttttg ccttagccgg gggctgagca
    18301 gcgttaccac ttttgttcaa ttgcccccgc tttcagcaac tccacatcct tgccgttaat
    18361 ggttgccgtt gcggcaacga ttgtggtcct tacagttgca caagattttc gctcaaattc
    18421 cggtttataa agaataagag acgaattact ggtaaatgaa ccaaataggt aatcagtaaa
    18481 gctaatattc agccggttta aaccgaaagg cgcgaagcca accgtttggt taatctcctt
    18541 accggatggg cgacgaatgc tgaatgtcac gcgaagattg gccacttcgc ctaatttatt
    18601 ggtggtggcg aagaggctat ccatgcagga aaaaccttta tctaaaaaat tatctcgcat
    18661 cccagaacgg gtaaccgtaa tcacgggtaa cgtatcgtca accgtttttc tgtcaatcat
    18721 gtcttttcga caagtagcca gaaagtcttc cgggccacca taaatgtgga cacctttatc
    18781 aagatattcc ttcgctttct tacaatctac ctcaacatac tgaccattgg cgtacataat
    18841 gccc
//
LOCUS       DAJTVK010000079        18524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000079 DAJTVK010000000
VERSION     DAJTVK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.079"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1710
                     /locus_tag="OO372_002766"
     CDS             <1..1710
                     /locus_tag="OO372_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6342216.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="OO372_002767"
     CDS             1711..3360
                     /locus_tag="OO372_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6342217.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="OO372_002768"
     CDS             3365..4141
                     /locus_tag="OO372_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6342218.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OO372_002769"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="OO372_002769"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCR6342219.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="OO372_002770"
     CDS             complement(5430..6752)
                     /locus_tag="OO372_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCR6342220.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OO372_002771"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="OO372_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCR6342221.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OO372_002772"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="OO372_002772"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCR6342222.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OO372_002773"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="OO372_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCR6342223.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OO372_002774"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="OO372_002774"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCR6342224.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="OO372_002775"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="OO372_002775"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6342225.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OO372_002776"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="OO372_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6342226.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OO372_002777"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="OO372_002777"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6342227.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4534 c         4867 g         4597 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cttggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gctt
//
LOCUS       DAJTVK010000080        18513 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000080 DAJTVK010000000
VERSION     DAJTVK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.080"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="OO372_002778"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="OO372_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6342228.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="OO372_002779"
     CDS             735..1040
                     /locus_tag="OO372_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342229.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="OO372_002780"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="OO372_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR6342230.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="OO372_002781"
     CDS             1788..2534
                     /locus_tag="OO372_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6342231.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="OO372_002782"
     CDS             2534..4630
                     /locus_tag="OO372_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6342232.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="OO372_002783"
     CDS             4676..5815
                     /locus_tag="OO372_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6342233.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="OO372_002784"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="OO372_002784"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6342234.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="OO372_002785"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="OO372_002785"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR6342235.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="OO372_002786"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="OO372_002786"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6342236.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="OO372_002787"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="OO372_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6342237.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="OO372_002788"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="OO372_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6342238.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="OO372_002789"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="OO372_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6342239.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="OO372_002790"
     CDS             complement(12873..13730)
                     /locus_tag="OO372_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6342240.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="OO372_002791"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="OO372_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6342241.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /locus_tag="OO372_002792"
     CDS             complement(14122..14709)
                     /locus_tag="OO372_002792"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6342242.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="OO372_002793"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="OO372_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="OO372_002794"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="OO372_002794"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6342243.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="OO372_002795"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="OO372_002795"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6342244.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4543 a         4912 c         4783 g         4275 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataata
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc tgttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctgccg aggtactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaattc cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gctgccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggtg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAJTVK010000081        18214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000081 DAJTVK010000000
VERSION     DAJTVK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.081"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            15..1091
                     /locus_tag="OO372_002796"
     CDS             15..1091
                     /locus_tag="OO372_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6342245.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="OO372_002797"
     CDS             complement(1484..1825)
                     /locus_tag="OO372_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6342246.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="OO372_002798"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="OO372_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCR6342247.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="OO372_002799"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="OO372_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6342248.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="OO372_002800"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="OO372_002800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6342249.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="OO372_002801"
     CDS             3559..4215
                     /locus_tag="OO372_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR6342250.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="OO372_002802"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="OO372_002802"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6342251.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="OO372_002803"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="OO372_002803"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6342252.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="OO372_002804"
     CDS             complement(7167..7826)
                     /locus_tag="OO372_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6342253.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="OO372_002805"
     CDS             complement(8153..8509)
                     /locus_tag="OO372_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6342254.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="OO372_002806"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="OO372_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCR6342255.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="OO372_002807"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="OO372_002807"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6342256.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="OO372_002808"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="OO372_002808"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6342257.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="OO372_002809"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="OO372_002809"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6342258.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="OO372_002810"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="OO372_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6342259.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="OO372_002811"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="OO372_002811"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6342260.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="OO372_002812"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="OO372_002812"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6342261.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="OO372_002813"
     CDS             complement(17270..18112)
                     /locus_tag="OO372_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCR6342262.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4672 a         4575 c         4391 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc accgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAJTVK010000082        18189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000082 DAJTVK010000000
VERSION     DAJTVK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.082"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO372_002814"
                     /gene_synonym="dgcC"
                     /pseudo
     CDS             complement(26..871)
                     /gene="adrA"
                     /locus_tag="OO372_002814"
                     /gene_synonym="dgcC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
     gene            complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO372_002815"
     CDS             complement(973..1293)
                     /gene="psiF"
                     /locus_tag="OO372_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR6342263.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO372_002816"
     CDS             complement(1412..2827)
                     /gene="phoA"
                     /locus_tag="OO372_002816"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6342264.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO372_002817"
     CDS             complement(2928..3188)
                     /gene="iraP"
                     /locus_tag="OO372_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6342265.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(3370..3573)
                     /locus_tag="OO372_002818"
     CDS             complement(3370..3573)
                     /locus_tag="OO372_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342266.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            3651..4745
                     /gene="ddlA"
                     /locus_tag="OO372_002819"
     CDS             3651..4745
                     /gene="ddlA"
                     /locus_tag="OO372_002819"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6342267.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(4769..4981)
                     /locus_tag="OO372_002820"
     CDS             complement(4769..4981)
                     /locus_tag="OO372_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6342268.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            5241..5549
                     /locus_tag="OO372_002821"
     CDS             5241..5549
                     /locus_tag="OO372_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6342269.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO372_002822"
     CDS             complement(5608..6702)
                     /gene="yaiW"
                     /locus_tag="OO372_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6342270.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO372_002823"
     CDS             complement(6715..7935)
                     /gene="sbmA"
                     /locus_tag="OO372_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6342271.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            8287..9444
                     /gene="ampH"
                     /locus_tag="OO372_002824"
     CDS             8287..9444
                     /gene="ampH"
                     /locus_tag="OO372_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6342272.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO372_002825"
     CDS             complement(9445..10068)
                     /gene="iprA"
                     /locus_tag="OO372_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR6342273.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(10156..13044)
                     /locus_tag="OO372_002826"
     CDS             complement(10156..13044)
                     /locus_tag="OO372_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6342274.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
     gene            13570..14544
                     /gene="hemB"
                     /locus_tag="OO372_002827"
     CDS             13570..14544
                     /gene="hemB"
                     /locus_tag="OO372_002827"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6342275.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO372_002828"
     CDS             complement(14650..15501)
                     /gene="tauD"
                     /locus_tag="OO372_002828"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6342276.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO372_002829"
     CDS             complement(15498..16325)
                     /gene="tauC"
                     /locus_tag="OO372_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6342277.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO372_002830"
     CDS             complement(16322..17089)
                     /gene="tauB"
                     /locus_tag="OO372_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6342278.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO372_002831"
     CDS             complement(17102..18064)
                     /gene="tauA"
                     /locus_tag="OO372_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6342279.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         4536 a         4728 c         4395 g         4530 t
ORIGIN      
        1 tatcaataag ttggagtcat tacccgctac cgcgcagggt aatttgtaac tgtcgggtaa
       61 gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg ctcttgaaat
      121 ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag ttatcaaatt
      181 cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg ccgtcgcgag
      241 tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc tggtagctga
      301 cccagccaaa cagcagagga taaacgacaa taatgggaag ggagagccac cattccagcg
      361 gcgcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg caggaaacca
      421 ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc aaattcagac
      481 acataatcat caacatagcg gtggaaggca gcacgtttac gcccattacg cctacccaca
      541 ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc ggatcgacgg
      601 ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg accaacacca
      661 gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt aagaacatgc
      721 cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc ccggaccgct
      781 gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct tttttgaaaa
      841 agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa attatagaga
      901 cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct cacccgttag
      961 cactatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat tacgatcatc
     1021 acctttcaga gattgttgcg ttgcttgatt attacattcg cgcatctttt gctgctgtgg
     1081 cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac tcatgtaggt
     1141 cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg aggtcatacg
     1201 ctgttgttgt ggggttaagg ttctctcggc agcgccgacg gtggttaaaa aaaccagacc
     1261 aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca tgttttaacc
     1321 atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa aaaaaccacc
     1381 cggcagcgaa aattcactgc cgggcgcggt tttatttcag ccccagagcg gctttcatgg
     1441 tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc ggaccatacg
     1501 ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcggaa ttcccgtaac
     1561 tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc ggcgctttgg
     1621 tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg accagcgtgt
     1681 taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga tcgaccgtct
     1741 cgccaatttg cccacaagga ttcgcagcgt gatcctgttt atcgattgac gcaccttcaa
     1801 cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggttttg tcggtcatct
     1861 gtgccagggt tggtacactg tcattacgtt gcggattagg cgtacaggtg actgcgggct
     1921 tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc atattgccgt
     1981 cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa ttcagtgagg
     2041 cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc gtttttccct
     2101 gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca agcgtaacgt
     2161 cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt tccagagcgt
     2221 tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa gtcacatgtg
     2281 ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta ccggtcgcca
     2341 gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga atatcgacgc
     2401 ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat gcagccgagt
     2461 cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg tattgcccgg
     2521 taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca taattacgtg
     2581 cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata ttttttgcag
     2641 gtttatcgct aagagaatca cgcagagcgg cggtctgatc actcgttaag cggcgagcac
     2701 cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc atttctggtg
     2761 tccgggcttt tgtcacaggg gtaaacagta acggtaagag tgccagtgca atagtgcttt
     2821 gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa gcgactataa
     2881 gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta ctgacgagga
     2941 tgcttcaata acttctttac gtaatcgcgc agcagctccg tatcgtcgtc aggaatgctg
     3001 gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg ctgttggtca
     3061 ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc tacctgcgca
     3121 cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc agcaatgaga
     3181 tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat gtttcacaac
     3241 ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc aaatattttt
     3301 tgtttgatta ccagcgaaac gtttctctta tttaaataat tacccacaca aaatataggc
     3361 ttttaattat caacctttat tataaataaa agttatgaaa tatattctct tcgttaactt
     3421 ataattaaga gaaaaaaact caccgcattg ccatttaagc caggaataaa ttagcttccc
     3481 gttcatcatt tttgtgatct cgcgcacatc tttgagataa cgcactttct tcaccagtac
     3541 gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt
     3601 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
     3661 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
     3721 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
     3781 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
     3841 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
     3901 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
     3961 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
     4021 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
     4081 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
     4141 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
     4201 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
     4261 acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga
     4321 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct
     4381 gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag
     4441 atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg
     4501 cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     4561 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     4621 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     4681 cacgtttgat tgagctggcg ctgaagcgtc acgctgcgga taacgcattg aaagccacaa
     4741 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     4801 gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     4861 aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata
     4921 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     4981 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     5041 tagacaacat tttcgtaatt gttaataaat ttaacttaag gttgccattt tgtaactcac
     5101 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     5161 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     5221 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     5281 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     5341 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     5401 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     5461 ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat
     5521 ggctgcactg gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc
     5581 cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     5641 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     5701 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     5761 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     5821 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     5881 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     5941 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     6001 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     6061 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     6121 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     6181 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     6241 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     6301 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     6361 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     6421 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt
     6481 ttccagcgtc ggtgccagcc cctgactttc aaaggtgatg gcgagatctt tcgcccaggc
     6541 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     6601 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     6661 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     6721 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     6781 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     6841 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     6901 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
     6961 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     7021 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     7081 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     7141 cagtttgatc cctaccactg ccagccatcc ggtccccatc agcgaccaga cgatagcggc
     7201 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     7261 cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     7321 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc
     7381 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     7441 gcggaacaca tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     7501 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     7561 cgaacttagc gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac
     7621 cccgacttcc accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca
     7681 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     7741 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     7801 gatctgcccg gaagcgccgg tgatacgcgc cacccagtct cccccaccgg cttgccagaa
     7861 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgctcccg gctttgggaa
     7921 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     7981 taaccgcatg accactacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     8041 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     8101 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     8161 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     8221 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     8281 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     8341 gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat
     8401 gccgatcata ttttttacgg cagcggtgca acggggatgg cgctggttgt tatcgacggt
     8461 aaccagcgcg tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
     8521 ctggattcgg tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg
     8581 aaattgctcg accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca
     8641 ggcgcacgcg tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat
     8701 accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     8761 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     8821 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg
     8881 tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa
     8941 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt
     9001 gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     9061 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     9121 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     9181 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
     9241 gagggtcgtc cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg
     9301 gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg
     9361 cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag cgggaataaa
     9421 ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     9481 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     9541 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg
     9601 caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt
     9661 atgcccaatg agctgcacgt cgcgtaattc cagaattcta ttttgccaag ctaaccagta
     9721 aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag
     9781 atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa
     9841 accatcagcc agccccataa tataaggggc ctgggtaata ccgataagta cgttttcttc
     9901 tctacgcaga gagataactc cctctaaaat aacgaaagta tcttcgttat tgacatcact
     9961 ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca
    10021 tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt
    10081 tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac cagtatttct
    10141 tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc
    10201 tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct
    10261 gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc
    10321 accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg
    10381 agtcagagcc tgatcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata
    10441 acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc
    10501 accagactgg aacagaccag aaacgctgcc gtaaggagtc acgtaaccag catcacccag
    10561 tttgaagtcg taaccggctt tcaagccgaa gccccaagcg tcggagttgg tgctaccgtc
    10621 aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag agtagctcaa
    10681 gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg
    10741 gctgtcttga tctacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc
    10801 cgcaccgacg atccacttag cgttgttacc gtcaactttg gtatcaacac cgaccatgat
    10861 gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa
    10921 gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt
    10981 accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc
    11041 catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc
    11101 agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttggt
    11161 tttggtatca tcgatataga tgagctgctt acctgcgtaa tcagcaatcg caccagcgcc
    11221 agtggagttg tcgatacgaa ctttgtagtt accggttgca gctacaacgt tgtctgcaga
    11281 gctgttgtca acttcgtcgc cattgttaat gtcgttgtta ccattaacgg tcaagtgacc
    11341 atcagagttc atcgcaataa caccataacc gtagttagct ttgctggtgt cgttggtacg
    11401 atcgttgacg aggtctgcat tcaggatgta atcattacta tgaatgtcaa acacacctgc
    11461 gtaaacatta ccgttgttat ctttaacgtt gccactggtc agttccagag tatcagttgc
    11521 aaatacgctg gagatgtcat tcatgttggt gtttacatcc agcacaccac cgttagtaac
    11581 ggtaatggta ttggcataca gcggtgcata tgcccaagct gaagcagtat cttcaccgaa
    11641 gttaacggtt gagaaggtat caatggtcag gtgatcggtg ttaacttcac cgttaacttc
    11701 cattgaagaa ccaccgttca gagtgatggt gtcagaagtc agagaagaat ctgcactcac
    11761 gttaacctga gaaccgctgt tgatcgccag ggtatcgatc agggaagttt tcgtggtttc
    11821 ccaatcagaa ccgttgttca gaacaacatc gaatacgccg ctctggaatg cgttagcgtt
    11881 atcaacacga ttccagccat cttcagagta agaagcgtac gggttcaggc tgtttggtgc
    11941 aacgtcgtag aaggcttcgt cagcaacaac aacgtcataa tcaatattcg ctgaaccaac
    12001 ccatttggtg ttgttcagag tcaggttcag gtgatcgacg ttctgatcgt cagttgccca
    12061 accgccatga gtgtaagcag tggtagagtt gtcaatcagg tgacccgtcg cgtcaaagtt
    12121 agcgttccag gtactgtcaa atacaacgtc accaagcaac tgagagttgt tgaacgtaac
    12181 gttgttgacc atacgataat cagaagtgtc atcatcgatg aaggctaacg caacatcatc
    12241 agcaggagat gcgccattgc cgtagtcact tggttcaaca gaattgccga aatgaccacg
    12301 atctgattgt tcgtcttcag tccatgaacc agaagttaca gtagagtcat taacaacgat
    12361 gttgttgtcc cactgatgac cttcggtatt gtaggtcgca ttaccgaggt cgtgttgaac
    12421 caaagtaata cctgcaacat gggagttgtt gctgatgttg atgttacttt caacatccag
    12481 agtcacagcg gtaccaagac cagcggttac caaacgatca taatctgtct tagatgtttt
    12541 accgtctgca tccagataag tatcggtgaa gtagaaggcg tcaaaatcat cgtcaatagt
    12601 agaattagcg atgttcagag tgaagatgtc gccatcaacc cagtcgtggc cagtataatc
    12661 gctaccgttg gtccagacgt agtcgccata accaacctgg taagatgtaa ccataccatg
    12721 aacaattgag ttggagatgt tcagtgtatt accggtatct gtgccataat cgtggttcag
    12781 ataataagta gaaattacac catttacagt gtcgccgctg aaagtggtat caaagtaacc
    12841 atcagctgcg tggtcatata cataaccacc gtaagtcaga taatcgtgat caattttagt
    12901 gctgtcaccc caattgtaga tggtggctgc gtcatcgtga tacccgaagg tgttataagt
    12961 agctgcatta gcctgagagg tgatagccag agtgcaagcc aatgctaatt gtgatactac
    13021 aagtttcttt ttccaggagt gcatttgtca tccctcctca gggacgtaat aagttgatcc
    13081 atcaattatc aatgcataga aaacatatta tgtttcgcgc taacgattat gctgcgttac
    13141 agacgaatgg ttcaattatt ctttccccta agtccgaatg tggacatata taatttatgg
    13201 agaaaaacac gcatcaaatt gataaaagga cgatatttca acatgttgca gataatgtta
    13261 tttttgatac atacgcgtta taaatatcaa aaacacaaac cagataaata tcttatcaat
    13321 atacaacaaa gtttgaatta ttcaatttat agcagcacaa ccaaacgcca aaaaaaataa
    13381 taaccaagag ctataaataa agattaaaaa tattttcagt gaatatgaaa ttattgctat
    13441 ttctattaca aagatgtcct ggtctctctt tcattctctt cttgtcaaca tcacgacaac
    13501 tttcgtaaaa cactcctgcc ccgcttcagg tatactgtgc accttaattc cacaaacatc
    13561 aggcagacca tgacagactt aatccaacgc cctcgtcgcc tacgcaaatc tcctgcgctg
    13621 cgcgctatgt ttgaagagac aacacttagc cttaacgacc tggtgttgcc gatctttgtt
    13681 gaagaagaaa ttgacgacta caaagccgtt gaagccatgc caggcgtgat gcgcattcca
    13741 gagaaacatc tggcacgcga aattgaacgc atcgccaacg ccggtatccg ttccgtaatg
    13801 acttttggca tctctcacca taccgatgaa accggcagcg acgcctggcg ggaagatgga
    13861 ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc cagaaatgat cgtcatgtca
    13921 gacacttgtt tctgtgaata cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc
    13981 gtcgacaacg acgcgactct ggaaaattta ggcaagcaag ccgtggttgc agctgctgca
    14041 ggcgcagact tcatcgcccc ttccgccgcg atggacggcc aggtacaggc gattcgtcag
    14101 gcgctggacg ctgcaggctt taaagatacg gcgattatgt cgtattcgac caagttcgcc
    14161 tcttcctttt atggcccgtt ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa
    14221 agctatcaga tgaacccaat gaaccgtcgc gaggcgattc gtgaatcact gctggatgaa
    14281 gcccagggcg cagactgcct gatggttaaa cctgctggag cgtacctcga catcgtgcgt
    14341 gagctgcgtg aacgtactga attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg
    14401 atgattaagt tcgccgcgct ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc
    14461 ttaggttcaa ttaagcgtgc gggtgcggat ctgattttca gctactttgc gctggatttg
    14521 gctgagaaga agattctgcg ttaatttttt tcctctcaga aagggggagt taacctgttg
    14581 aaagtacggt gaacgccccc tcaccctaac cctctcccca agggggcgag ggaaccgtcc
    14641 gcactcgtat caccccgccc gataaaacgg tttatcccca aggatcgtcg cccgatgcat
    14701 aatccgccgc tgtggcaggt aatcggcatt ggcatagtgc tgagtcacgc ggttatccca
    14761 aatcgcaata tcattcggtt gccagcgcca acgcacctga aactccggtt tagtgatatg
    14821 ggcgaataaa aagcctaaca aggcttcgct ctctttctcg ctgacatcga caattcgcgt
    14881 agtaaagcct tcattcacaa acagcgcctg tttaccgctc accggatgcg ttcgcaccac
    14941 cggatgtagc aacggaggat ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc
    15001 ggttttgcgg tatttgtatt ccgggaacga tttacggaaa tcatgctccg cacgcagccc
    15061 gctcagcagc tggcggaagg gaacagagag cgcctcatag gccgcaatac cgctggtcca
    15121 gagcgtatca ccgccggtcg aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg
    15181 cgtttcaata aatgtcacat cggtatgcca gttgtcgtta tccggcggat tatcgttatg
    15241 ggtatccagc acgatgatct cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg
    15301 cagttcgcca aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg
    15361 cagaaatacc acctgatagc gcagcaccgc atggtaaagc tgttcaaact gattatcgct
    15421 taacgggcgc gtcaggtcgg cacccgaaat ttgtgcgccg atatacggcc ccagcggggt
    15481 aatactcaga cgttcactca ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac
    15541 gcgcgcagac ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt
    15601 accacgtcag tcgcgagaaa ttcacccgct gactgaacca taaatcctaa accgcgtgtc
    15661 gcggcaatca gctccgccgc taccagcgta gaccagccca cacccagacc aatgcgtaat
    15721 ccggtgagga tttccggcag cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg
    15781 ctggcaccca gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc
    15841 agcaccgaca tcgccaccgg tgcaaaaatc gctaaataga tcaataagat cttcgaggtt
    15901 tcaccaatac caaaccagat caccatcagc ggcaaataag ccagcggcgg caccggacga
    15961 taaagttcga ttaccggatc cagaatgccg cgcaccgtcg ggcttagccc catggcgatc
    16021 ccgaccggaa taccgatcac caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg
    16081 gctgccagat gctgccacag cgtggcgtcc ataaatccct gcggtccggc aatggtgagc
    16141 agttttgcca gtacttgttg cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc
    16201 gccaccgtcc accatacagt gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga
    16261 cgcgagagcg gccagcgcca tttcagccgc cgcgaatgca gtttttcatt aatgagcaca
    16321 ctcatgagaa cgcctcccgt tgctcaaata cgcggcttaa aacatattcg cgcatggcga
    16381 tgaattgtgg atcggacttg atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa
    16441 agttgagcgg cagccgctcc agcacacggc cagggccgga tgaaagcaga accagttcag
    16501 tcgccataaa caccgcttct tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct
    16561 cctgccagag tttcagcagc agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac
    16621 caaacggttc gtcgagtaat aacagctggg gattcgccgc cagcgcgcgg gcaatcccca
    16681 cccgctgacg ttgaccaccg gaaagctgcc agatatagcg tttttctgcg ccttccagcc
    16741 ccactttttt cagcatctgg tgcgcgattt ccagtcgctg cattttctct atacctgcca
    16801 attgcaggcc gaacgccacg ttgtcctgta cattgcgcca cggtagtagc ccttcattct
    16861 gaaaaaccac cccgcgctct gctcccggtc cctcaatacg cttacccgcc agttgaatgc
    16921 tgccatgctg ataaggcaca aaaccggcaa tcagattcag cagggtggtt ttaccgcagc
    16981 cggacggccc cagcaccacc agtagctcgc cgctttccag cgtcaggttg atatcttcca
    17041 gtgccggttt gccgccataa tcggcataga gatgagagat ttgcagcatc tgcgcctcct
    17101 tttattgcac gaagcgcgag gtaacgtact ggctgtaatc attcgctaca gccgggacct
    17161 tgccctgctc tttcaaaaac tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca
    17221 gttctgccgt ttgttgctgc ggcgtcagat aggtattccc cttcaccagc cccggaacgt
    17281 caccttccgg cacgccgctt aaacgcgcca gtttgctgat gttttccggc tgtttcagcc
    17341 acgcgtctgg gttagcaatg tacggttgct gagcatcgat ggcgctttta gcgaacgctt
    17401 tcacgacctc aggatgtttc tcggcaaaat ctttgcgcac cacccagacg tccagcgttg
    17461 gcgcacccca ctgcccgacc tgttcagaat cggtcaacac cttgccgtct ttttccaggg
    17521 cgttaaccgc cggtgcccag acataagcac catcaatatc accccgctgc catgcggcga
    17581 taatcgcggg cggctgcagg ttcacaatct ccacttgccc tggtttaatc ccccagtgtt
    17641 tcagcgccgc cagcaggctg tagtgggtgg tggagataaa cggtacggca atgcgtttgc
    17701 caatcaaatc ttcgggtttg ctgatagttt tctttaccac cagcgcttcg gagttaccca
    17761 gttttgacgc cagcaagaag acttcaatcg gcacctgttg gctggctgca accgctaatg
    17821 ggctggaacc gaggttgccg atttgcacgt cgccagaagc cagcgcccgc acgatgctgg
    17881 ctccgctgtc aaacttacgc cagtccacgc tcgccccgct ctctttagca aaggtgttgt
    17941 cggcctgagc cactttcgcc ggttcggctg aggtttgata cgccacggtg acgttcaccg
    18001 cctgtgcctg aaaagcgatg aatgccagtg cggcaagaag tgtgttacgc gatgaaattg
    18061 ccatgattgt ctgctcccct gtcttgttat gggagcagta ttcaggaata aaaacattca
    18121 ttaaaagaat tagtcgttat cgcacagatg attttattct tagcaaaaaa acggcaaagg
    18181 aaagattaa
//
LOCUS       DAJTVK010000083        17957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000083 DAJTVK010000000
VERSION     DAJTVK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.083"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="OO372_002832"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="OO372_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6342280.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="OO372_002833"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="OO372_002833"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6342281.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="OO372_002834"
     CDS             4388..4639
                     /locus_tag="OO372_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6342282.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="OO372_002835"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="OO372_002835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6342283.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="OO372_002836"
     CDS             5944..6702
                     /locus_tag="OO372_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6342284.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="OO372_002837"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="OO372_002837"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6342285.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="OO372_002838"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="OO372_002838"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6342286.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="OO372_002839"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="OO372_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="OO372_002840"
     CDS             complement(9556..10176)
                     /locus_tag="OO372_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6342287.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="OO372_002841"
     CDS             complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="OO372_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6342288.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="OO372_002842"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="OO372_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6342289.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="OO372_002843"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="OO372_002843"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6342290.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="OO372_002844"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="OO372_002844"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6342291.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="OO372_002845"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="OO372_002845"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6342292.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="OO372_002846"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="OO372_002846"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6342293.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="OO372_002847"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="OO372_002847"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6342294.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4315 a         4749 c         4488 g         4405 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagtgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttgggcg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccaccag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcaatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gctgcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc cccctgccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgct
//
LOCUS       DAJTVK010000084        17854 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000084 DAJTVK010000000
VERSION     DAJTVK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17854
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.084"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO372_002848"
     CDS             complement(<1..304)
                     /locus_tag="OO372_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6342295.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(514..1032)
                     /locus_tag="OO372_002849"
     CDS             complement(514..1032)
                     /locus_tag="OO372_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6342296.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1729..2229
                     /gene="tssB"
                     /locus_tag="OO372_002850"
     CDS             1729..2229
                     /gene="tssB"
                     /locus_tag="OO372_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCR6342297.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            2264..2488
                     /locus_tag="OO372_002851"
     CDS             2264..2488
                     /locus_tag="OO372_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342298.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            2539..4014
                     /gene="tssC"
                     /locus_tag="OO372_002852"
                     /pseudo
     CDS             2539..4014
                     /gene="tssC"
                     /locus_tag="OO372_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            4021..4434
                     /gene="tssE"
                     /locus_tag="OO372_002853"
     CDS             4021..4434
                     /gene="tssE"
                     /locus_tag="OO372_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6342299.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            4438..6288
                     /gene="tssF"
                     /locus_tag="OO372_002854"
     CDS             4438..6288
                     /gene="tssF"
                     /locus_tag="OO372_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6342300.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMQPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            6252..7334
                     /gene="tssG"
                     /locus_tag="OO372_002855"
     CDS             6252..7334
                     /gene="tssG"
                     /locus_tag="OO372_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCR6342301.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            7359..8639
                     /gene="tagH"
                     /locus_tag="OO372_002856"
     CDS             7359..8639
                     /gene="tagH"
                     /locus_tag="OO372_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCR6342302.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            8636..9160
                     /gene="tssJ"
                     /locus_tag="OO372_002857"
     CDS             8636..9160
                     /gene="tssJ"
                     /locus_tag="OO372_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCR6342303.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            9163..10492
                     /gene="tssK"
                     /locus_tag="OO372_002858"
                     /pseudo
     CDS             9163..10492
                     /gene="tssK"
                     /locus_tag="OO372_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            10497..11258
                     /gene="icmH"
                     /locus_tag="OO372_002859"
     CDS             10497..11258
                     /gene="icmH"
                     /locus_tag="OO372_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCR6342304.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            11267..14050
                     /gene="tssH"
                     /locus_tag="OO372_002860"
                     /pseudo
     CDS             11267..14050
                     /gene="tssH"
                     /locus_tag="OO372_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            14047..14790
                     /gene="tagO"
                     /locus_tag="OO372_002861"
     CDS             14047..14790
                     /gene="tagO"
                     /locus_tag="OO372_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCR6342305.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            14795..16207
                     /gene="tssA"
                     /locus_tag="OO372_002862"
     CDS             14795..16207
                     /gene="tssA"
                     /locus_tag="OO372_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCR6342306.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            16226..>17854
                     /gene="tssM"
                     /locus_tag="OO372_002863"
     CDS             16226..>17854
                     /gene="tssM"
                     /locus_tag="OO372_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCR6342307.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPYV"
BASE COUNT         4154 a         4896 c         4879 g         3925 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 tcgtcaggta ggccattttg tccagtacct gcggaaattc aagggagaga aactgctggc
      181 tgaccagaga gagctcgagg gaaaagagcg aagagagtga ctggttcaga tgaaaggaga
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag cgtgaaacgt aatccggttg
      301 acatagcaac ctccggggtt aaggaaaaac agacagttcg ctgttaaagc ggctgtgaca
      361 gtacagtgtt acgcttgcgt tcaggcacgc taccgccgaa gtttccggca tccgccatct
      421 cccgtgaacg taacccctta ctgacaacct ttctttctac ggcgtaatac aaaaacagca
      481 tccggcctga tagccggatg ctgttggctt aacttatgct tccagtggtg cgcgccagtc
      541 gtcggcaccg gaggtgccag atttgacgtg ttcccattca attttgcggt aggccagtga
      601 aacctcgagc aactgggtga actcgcgttt cgccgggtcc tggcaatgcg gcatatgcag
      661 tttcatatcg acaatcgtcg aatcagtcag gcgggtagtg aaataatgct cctgtttgcc
      721 ctccacagag gtacgccacc agtgaagttc agtggttggc agcatttcgc cggaggccag
      781 tgcgttgtac atcagcggaa ccgctttgtt cagcgccacg gtaaaaatga aaggcttgtg
      841 agcacgctga cccgcaggct gaccggactg aggagtggtc gggacagtaa cgttatgcag
      901 gaattcctgc accagcatct cgtcttcgtg cccctgcaca tagatgttgc cgacagaatc
      961 agcggtaaat gcacctgcag taatatttcc ctgggtctga ccagtgatgg agatataaca
     1021 cggtgttggc atttgtaaac tccttgttaa cgtggttaat tactacttac aatgacctgc
     1081 cgttaatccc agacaggcag atgcagctca cttcacggaa ccgcatcatt tttcagtatt
     1141 ttttacatac aaaccgagct agaatttacg gttgtcgtaa aaactatttt taaaatcatt
     1201 aaatctctta ttcagcaata acttaataat atatatctgg ataaccatac ccaactgttc
     1261 aaagtccgta ttgcctccat aaaatggcca aagctgaatc atgaataaat attgtattta
     1321 tcctttctga attatcattt tgataatttt tgagatattc cttaaaaata cccacttaat
     1381 caatcacaaa acaaatatat aaacaagcaa tcattagatt agcttatttt taatgaaatt
     1441 attatatact tcaataagtt acacaaaaac tctatattta aattattgcc ataatacacc
     1501 tgaagcgaat tgcacaggtt cacacaatct atttaacgct tgcagaatac ccctctttac
     1561 gatcgataaa aagtcctttt cttatttatt ttgaagcgta aaatgcgggg tgatgaagga
     1621 gtggtgattt tattttcacc gccattaggg cggcattgcc gatatttttt tgcggtacaa
     1681 atatgccgtg ttcatggcct gtacgagata acatgacgga tcacttttat gagcaaaaaa
     1741 tttgaaggca gcgtcgcgcc ccgcgagcgt attaatatat cgtatgtccc aaaaacagat
     1801 ggccagacgg ctgaagtgga acttccgctt aacatgctcg ttgtcggcga taccggaaat
     1861 acgcaggaaa catccccact ggatgaacgt caggcagttt ccgttaacaa gcacaatttc
     1921 ggtgcagtga tggctgaagc agccattggc ctcaatttta ctgtccccgc cacgctgaag
     1981 ggaagcacaa cagacgatga actgaatgtg gcgctgaata tcaaatcact ggatgatttt
     2041 tcaccagata gcgtggcccg gcaggttccg gaagtgaata aattgctgga actgcgtgaa
     2101 gcactgactg cactgaaggg gccgatgggt aaccttccgg cattccgtac ccagctccag
     2161 gcattgctgg aaaatgaaga gtcccgcgag cagttgctca aagaaatcgg tctggtgagc
     2221 aataaataac caccactatt tcattaaaca gggttatcca ggcatgacgg cacacaaaaa
     2281 tatctccggg acataccacc tctctgttgc agacattctg caggtggttt accaggtatg
     2341 tttcagcccg tccgttgaaa tcaaccagga tggtgtggct gcgctgatta caacactgga
     2401 tcgccgtatc agtgatctat tggatgagat tatccatttt tgtgagtttc agcagagcgc
     2461 gtcgcactgg cagcgcgtac ttcactgatg gcgaaaagcc ggtttcagaa acacagctaa
     2521 aacagaagga ttttcagtat gtctttacag gaagaggaac tcgtttccag tcatgccggg
     2581 cagcctgagc aggcatcgtc gctgcttgat caaatcatgg cacagacccg tattcagccc
     2641 ggttcggaag gttatgacgt tgccctccag ggtgtgacag cctttattgc cagcatcctg
     2701 caatccactg catctgccga accggtcaat aaactggccg tggacagcat gattgcagat
     2761 attgatgaac gcatcagccg ccagatggat gtcatcatcc atgcgcctgc ctttcagcag
     2821 gtcgagtcgt tctggcgctc actgaaaacc atggtggatc gcgttgattt ccgtgaaaac
     2881 atcaaggtca acgtgcttca tgtcaccaag caggaacttc tggaagactt tgagtttgcg
     2941 ccagaaatta ttcagtccgg attttataag cacgtttatt cgtccggttt cggacagttt
     3001 ggcggtaaac ccatcgctgc cgtgctgggc gcttatgagt tcaaaaatac cgcgcctgac
     3061 atgaaactgc tgcaatacgt cagtgccgtg ggcgcaatgg cgcacgcacc gttcctgtcc
     3121 tccgtttccc cggagtttat ggggctgaac tcgtggaccg aactgcctaa tatcaaagat
     3181 ctttatgcca tctttgaagg tccggcctac accaaatggc gcgccctgcg tgactcggaa
     3241 gattcgcgct atttagggct tacggcaccc cgcttcctgc ttcgccagcc ctattcacca
     3301 acagataacc ctgttaagaa ctttaactat tacgaagatg tcagccagaa ccacgaagat
     3361 tatctgtggg gtaatacggc ctggatgctg gcatgcaata tcgcagacag ttttgccaaa
     3421 taccgctgat gtccaaacat tatcggtccg caaagcggcg gcgcagtgaa agatctgccg
     3481 atgcatctgt tcgaaacgat ggggcagatt caggccaaaa tcccaaccga ggtgctggtc
     3541 accgatcgcc gcgaatttga actggctgaa gagggtttta tcacccttac catgcgtaaa
     3601 gactctgata atgcagcctt tttctctgca aactcggtac aaaaaccgaa gcactttccg
     3661 ggaaaagatg cagaaaccaa ctataagctg ggtacgcagc ttccttatct cttcatcatc
     3721 aatcggttag cgcactacat caaggtgttg cagcgtgaac aactggggtc atggaaagaa
     3781 aggagcgact tagaaagaga gcttaatacc tggatccgtc agtatgttgc cgaccaggaa
     3841 aacccgcctg ctgacgtgcg cagccgcaaa cctctgcgcg ctgccaaagt tgaggttatg
     3901 gatgtagaag gcgaacccgg ctggtaccag gttgcgctaa gcgtgagacc tcattttaaa
     3961 tttatggggg caaattttga gctttccctg gttggccggt tagacaggga gtaacacacc
     4021 atgataccgg atcgggaaag aaccacaggt tctctttttg agcgtatgga agcctcttcg
     4081 gccagaaacc ggcagggagg cagtattcat tcgctcaggc aatccattcg ccagaaccta
     4141 cgaaacattc ttaatacccg gtccggtagt tgccggggtg cgcctgagct gggcattgat
     4201 gaaccggaag gggctgaaaa cttccgcgaa tccatgagtc gggccatcga gcaatgtata
     4261 gaacgctatg aaccccggat ctcacacgct gaagtgcagg cagtggtttc atccgcctcc
     4321 agcccgctgg atatgacgtt tcatattaca gcctgggtga catttaacga aacgcatgag
     4381 gtgcttgaat ttgatatggc accgaatggc agccagcatt accgggtgga ctgacaaatg
     4441 gaatttgaag aacgctattt ccgggaagaa ctcgattacc tgcgccagct tagcaagctg
     4501 ctggcaacgg aaaaacccca tctggcccgc ttcctggccg aaaaagatgc agatccggat
     4561 attgaacgcc tgctggaagg ggtggctttt cttaccggca atctccgcca gaaaattgag
     4621 gatgaattcc cagaactgac gcacgggctt attaagatgc tatggcctaa ttacctgcgt
     4681 ccggttccgg caatgaccct tattgaatat acgccggata tggataagtc ttctgtaccg
     4741 gtgttaatcc cccgtaatga gcagtttaca accaacgccg gggaaatcag agttgatgaa
     4801 gtgctgccct ctgatgctaa aaaggaggag ccgcctccct gtaccttcac cctctgccgg
     4861 gatatctggc tgctgcccgt tcgcctggag cagattgaaa accgcagtac gacccgtaat
     4921 ggtgttatca acatcacctt ttcggtcgca ccgggaacgg acttccgcac gctggatctg
     4981 aacaaacttc gcttctggct cggcaatgac gacaactata cccgtgacca gctttattta
     5041 tggttctgcg aatacttgca gggtgccgac ctgactgtgg gtgaacagca tattcgcctg
     5101 cctgagttta tgctaaaagc tgtcggtttt gagccgcagg atgccatgca gccctggccg
     5161 aaaaacgtcc acagcggcta ccggatcctt caggagtatt tctgttaccc cgatgcgttt
     5221 ctcttttttg atctttgtgg ttgtccggct ttgcctgacg gattgcaggc ggaattcttt
     5281 accctgcaac tgcgtttttc gcgccctttg cccgtggaca tccggctgcg ccgcgattcc
     5341 ctgcgcctgt attgcgcacc tgccattaat ttatttatcc accatgcaga agccatcacg
     5401 ctggacaacc ggcgggcaga ctatccgctg gttcccagcc gccattaccc acaacattac
     5461 gatgtatttt ccgttaacag tgtggtgagc caggtccagg atatgttcag gaaaaaagat
     5521 ctggggcgtc ctgtttcgac gcaggccgcg cgccagtggc cagcctttga aagtttcagc
     5581 catcagatag aatacagccg gaagcgggaa gtggtgtact ggcatcaccg gaccaaaaca
     5641 tccctgttcc atcgcggctt tgatcatacc cttgccttta tacatgctga tggcagttat
     5701 ccgtcagacg aatctctgct cagtaatgaa gtggtttcgg tatcgctgac ctgtaccaac
     5761 cgtgagcttc cgtcacaaat tcgttccggc gatatcaccg gcacaaccgg aaaaaatgcg
     5821 gctgttgctt catttcgcaa cattacccgc ccgacgcaac cactctggcc ggtcattgat
     5881 ggcagcctgc actggcccct actctccgcc atgaacctga attatctgtc attactggat
     5941 acggacgcgc tgaagcaggt catcgccaac tttgatcgcc acgcaatcca tcatccgcag
     6001 acggcacggc tgtcacaaca aaagctggat gccattgagc gtctggagac ccgccccgtt
     6061 gatcgcctgt ttacgggtat tcccgtccgg gggctggctt ccacgctgta tctgcacccg
     6121 gagccgtttg tctgtgaagg ggaaatgtat ctgctcggta cggtgctttc gcattttctg
     6181 tcgctgtacg ccagcgttaa ctcattccac atgctgaccg ttgtgaacac agaaagccag
     6241 gagacatgga aatggacgga aagaatcggg cagcatcctc ttatctgagt ccgggaaatc
     6301 ccccggcaga taaagagcag aatgatccgc tggcacaggt tttccataat gcctgctctt
     6361 acaatttttt tgcgatggcg gagctgttgc accgcctggc aaagggtgaa aaggggacgc
     6421 cagaattatc cctgcgcgac gatccggcac aggaaaccct gcgtttcagc gccgatgcca
     6481 gccttgcctt cccgtgcagt gatatcagtg cgctgaaacg ggatacgtca ggggcattca
     6541 ggatgaccac cacctttatg gggttacagg ggagccagtc gcctctcccc ggttattacc
     6601 tcgctcacct ggcctggaaa gcggtacatg aacaaagtcc ggtaggtgat tttctggata
     6661 tgttcagcca ccgcctgacc cagtttgtct ggcatatctg gcgtaagtac cgctatcaca
     6721 tcagttttcg taacggcggt gtggatgcgt tctcgcagcg catgtactcc cttgtcgggc
     6781 tgggccaccg ccagcttcgc gataagctgg cgattaatca cagcaaaatg ctggcgtatt
     6841 ccgggatcct ggcaaatccg ggacgatccc cggaaattat ctgtggcctg gtgtcccact
     6901 gctttgattt gtcagaagtg acactgcaaa actggcaacg tcgcaaggtg gatattgaac
     6961 cggatcagca aaatagtctg gggagctatt ctcttaaaaa tggcgaaaaa ctggcgggaa
     7021 gatcggtact gggcaacttt gttctgggta cacgagtgcc cgatctcagc ggaaaattcc
     7081 agctcagtat taccagcctg acccgaaaac agttcctctc ttttctgccg tccggcgaaa
     7141 acttcctgcc actgacgatg ttcgtgtcct tcatcctgcg cgatcagctt gcctgggact
     7201 tacatctggg gctggcgccg gagcaggtgg gcgcaatgcg cctgggcgat aacaaaagtg
     7261 cgctgctggg ctggaccagc ttcctcggca caccggaaga acgaccctca gtcaccatca
     7321 gggtacggtc gtaaattatg ataatcacgg agcagaatat gccggaagag aaactacaga
     7381 cgctttcatt gcaggtcatt aacggcagtg agctggaaag cgggcgggcg gcgcgctgtc
     7441 tgttcacaca gcagggaaat gtcggccatg gccccgaatg ccactggtcg gtacaggatc
     7501 gtcagcagag cattccggcc caggctttta ccgttatcct gcacgatggc acattttgtc
     7561 tgcgcccgca gacggcacaa ctgtggctga atcaggcaaa agtcacagca acatcagacc
     7621 taatacagtt gcgccagggc gatgagatcc agatcggacg gctgatggtg agggttcatc
     7681 tgaaccgggg agatattccc cattacgatg aggaaatggc cactcccgaa accatcgtta
     7741 ccaatcgcga tatgctcacg gataccctgc tatcaacgga gggtgcgcca cactatccgg
     7801 gaatgactca ccggcaccag cttgcagaca ccgtggtaaa cggtttttct gccgatccac
     7861 tccaggctct tcagtccgaa agcctgatta ccacgggcga tccgctttca ggcattgcgg
     7921 ctgtccggcc atcggcaccg ctgtccgatc cggcaagtaa tggggggatc aatactccgt
     7981 ttatggatct gccgcccatt tatgccagcc ccggcgatcg taatgacgac gtctctgcgg
     8041 cagaaatggc acaacgccac gttgcggtca cccccttact gcgcggtctt ggcggctcgc
     8101 ttaccatgag caattccgac gatgcggatg attttctgga ggaggccgga cgaacgttac
     8161 aggccgcaat aaaaggtctg ctcgatttgc agcagcagcg taacagcctc tcagacaaac
     8221 atttgcgccc gctggaagat aacccgctgc gcctgaacat ggattacgcc accgcgctcg
     8281 acgtaatgtt tgccgaaggt aaaagcccgg tacatctggc ggctcccgcc gccgtcagtg
     8341 aaagcctgcg caatgtccgc caccacgaag aagctaaccg ggcagcgatt gtggagtcgc
     8401 ttcgtgtcct gctggatgct ttctcaccac aaaatctgct gcgccgcttt gtgcagtacc
     8461 gccgcagcca tgaactgcgc cagccgctgg atgatgccgg agcatggcaa atgtacagcc
     8521 attattacga agaactggcc tccgatcgcc agcaggggtt tgagatgctg tttaacgagg
     8581 tctacgccca ggtctatgac cgggtgcttc gtgaaaaaca gcgggagccg gaagcatgaa
     8641 caggaaggcg tttctggcct gcgtgctgat gtgcgtcata ttaaccggct gcgaaacggc
     8701 gaaaaaaatc agtgaggtga tcaaaaaccc ggatatccag gtcgggagcc tgaaggagca
     8761 accttcggaa ataaccgtca ccctgctgac cgaaccggac accaatacca atgcggaagg
     8821 tgaatcagcc gccgtggatg tgcaactggt gtacctgact gacgactcaa aattgctggc
     8881 agccgactat gaccagatag ccagcacgcc gctaccggat gtgctgggta aaaactatat
     8941 cgaccaccag gatttcaacc tgttgccgga caccatcaaa accctgccgc cggtaaagct
     9001 ggatgagaaa acgcagttta ttggtgtcgt ggcctatttc tccgatgacc aggccactga
     9061 atggaaacaa atcgagacgg tggaaggaat cggccaccat taccgcctgc tggtgcatgt
     9121 acgtcaaagc agcatcgaaa tgaaaaaaga ggatgaataa ttatggcaac cacccgcaac
     9181 aaggtgatgt ggcaggaagg gatgctgatg cgtccacacc atttccagca gcagcagcgt
     9241 tacaacgact acctggataa ccagcgtttc cgggccatga atgatttatc ctggggattt
     9301 accgaactca ccctcaacaa tgaactgctg gcgcagggta agatcatgat tgacagcgcg
     9361 tcaggcacac tgcccgacgg caccgtcttt tctatccccg accaggacgc actgcccgat
     9421 ccgctgcacc cgcaaaattt tccggacgaa agaagccgca atatctacct cgctctgccg
     9481 gtcgccagtg atgtgagaaa tgaaatcagc gacggacggc gaatcgggcg ttaccggctg
     9541 aattatgccg atgtccggga tttgcattca gaagaaggtg acgcgcgaac attgacgctg
     9601 ggtcaactga cgccgcgcat tatgagcggt gcagaagata tgagcgccta tattacgctc
     9661 ccgctttgcc gtatcagcga tcgccgccga tggctccctg acgttggatg acgattttat
     9721 cccctcctgc cagaatattc aggtcagtaa gaaactgcgt gtttatctca aagaggtaca
     9781 gggggccatt ggcggacggg caagcgatct ggcaaaccgt attggctctc cggcgcagag
     9841 cggcatcgcg gatgtggcgg aatttatgat gttgcagtta cttaaccgta accagacccg
     9901 gtttacccat cacgcccgtc gatcccagct ccacccggaa gatttctacc ttgatcttgc
     9961 cgggttgctg ggtgaactga tgacctttac cgagccgtcg cgcctgccct gcccgcttga
    10021 tgtgtatgat catcatgacc tgaccaaaat atttaaaacg ctgttaccgg aagtcaaacg
    10081 ggcgctgcat accgtactgt cgccaagagc cgtcaatctg ccgctgcatc tgcgtgacgg
    10141 tatctggcag gccgatatcc atgacacaga actgctgcaa tctgccacct ttgtgctggc
    10201 cgtggcggca aacatgccgg tcgatcagat ccagcgccag tttatccagc agtcgaaaat
    10261 ttcctcgccg gaaaaaatcc gcaatatggt cagtgtgcag atccccggta ttccattgcg
    10321 tgcccttatg gtggctcccc gccagcttcc ttaccattcc gggttcagct atttcgaact
    10381 cgacaagagt ggccaggcct ggacagaaat ggctgccgcc ggggccgttg cactgcatgt
    10441 ttccggcagt ttcccggatc tgaacatgca actgtgggcg ataagagggt aagacgatgg
    10501 atgaggggtc actgtctctg ccaccattta ccggacacga cgaaaaaagc cagcgcaact
    10561 accatctcgc gcttcgtggc aacagcctga atccgatgat cgacgcggcc acgccgctac
    10621 tcggtatggt aatgcgcctt tcaaccatga acagccagac gatgccggag catctttttg
    10681 ctcaggtggt gacagatgtt caggccgtgg agcaactgct tcaggagcag ggctacgaac
    10741 cgggcgtgat tatctcgttt cgctacatcc tctgcacgtt tattgatgaa gcggcgctcg
    10801 gtaacggctg gtcaaacaag aacgagtgga tcaagcagtc attgctggtc catttccaca
    10861 acgaagcctg gggtggcgag aaagttttca tcctgctgga gcgcctgatc cgtgaaccca
    10921 aacgttacca ggatctgctg gaatttctct ggatctgttt ttctctggga tttcgcgggc
    10981 gctacaaagt ggcagcacag gatcaggggg aatttgagca gatctaccgc cgtctgtacc
    11041 acgttctgca taaactgcgc ggcgatgccc ctttcccgct gttacaccag gacaaaaaaa
    11101 cccagggcgg gcgttatcag ctcatcagtc gcctgactgt taaacatatt ttttgtggtg
    11161 gcgttgtcgt gctggcgttg ttttacctgt tctatctgtt gcggctggac agccagacgc
    11221 aggacatcct gcaccagcta aacaaattac ttcgataagg acgtttatga tccagattga
    11281 tcttcccacg ctggtaaaac ggctgaacct gttctcccgc caggcgctgg agatggccgc
    11341 ctctgaatgt atgagtcagc aggcagcgga aattaccgtc agccatgtgc tcattcagat
    11401 gctcgccatg ccacgcagtg acctgcgggt tattacccgc cagggcgata ttggcatgga
    11461 agagttgcgc caggcgctga cggtagagaa ctacacaacc gcccgttctg cggacagcta
    11521 cccggcgttt tccccgatgc tggttgagtg gcttaaagag ggctagctgc tggcgtcggc
    11581 tgagatgcag cacagcgaac tgcgcggcgg cgtgttgctg ctggccctgc tgcattcgcc
    11641 gctgcgttat ataccgcctg ctgccgcccg gctgttgacc ggcattaacc gtgaccgtct
    11701 gcaacaggac tttgtgcagt ggacacagga gtcggcggaa tcagtcgtgc cggatgcaga
    11761 cggtaaaggc gcaggcacaa tgacggacgc ctctgacacc ctgcttgccc gctatgccaa
    11821 aaacatgacc gcagacgccc gtaacggcag gcttgacccg gtactgtgcc gcgaccacga
    11881 aatcgacctg atgatcgaca ttctctgccg ccgccgtaaa aacaacccgg tggtggtggg
    11941 cgaagcgggc gtgggcaaaa gcgcactgat tgaagggctg gcgctgcgca tcgtggcagg
    12001 ccaggtgccg gacaagctga aaaacaccga tatcatgacc cttgacttgg gcgcattgca
    12061 ggccggggcg tcggtgaagg gtgaattcga aaaacgtttc aaagggctga tggcggaggt
    12121 catttcctcc ccggtgccgg tcattctgtt tatcgacgaa gcacataccc tgattggcgc
    12181 gggcaaccag cagggcgggc tggatatctc caacctgctc aaaccggcgc tggcgcgcgg
    12241 cgagctgaaa accatcgccg ccaccacctg gagcgagtac aaaaaatact tcgaaaaaga
    12301 tgccgccctg tcgcgccgct tccagttggt gaaggtcagc gaacccaacg ctgccgaagc
    12361 caccattatt ctgcgcggtc tgtcggcggt ctatgaacag tctcacggcg tgctgattga
    12421 tgatgacgcc ttgcaggccg ctgcgacatt aagcgagcgt tatctctccg ggcgtcagtt
    12481 accggacaaa gcgattgatg tgctggatac cgcctgcgcc cgtgtggcca tcaacctgtc
    12541 gtcgccgccg aagcaaatct cggcgctgac cactctgagc caccagcagg aggcggaaat
    12601 tcgccagctt gagcgcgagc ttcgcatcgg actgcgtacc gacacatcac ggatgaccga
    12661 ggtgctggtg cagtatgatg aaacgctgac ggcgctggat gaactggaag cggcctggca
    12721 ccagcagcag acgctggtcc gggagattat cgcgctgcgc cagcagttac tgggcgtggc
    12781 agaggacgat gcggcgccgt tgccggacgc agataccgtg gaggatacgc agccagagtc
    12841 agagtcagag tcagagtcag agtcagagca ggataatacc ggtgccgaac cggctgatga
    12901 ggccgacaga gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc
    12961 ccagctcact gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt
    13021 cgataaaaaa cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct
    13081 gtcgcagaat gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa
    13141 aggccaggac ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct
    13201 gcgtcgtccg ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa
    13261 aaccgaaacc gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac
    13321 catcaatatg tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc
    13381 gggctacgtt ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta
    13441 ctcggtagta ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta
    13501 ccaggcgttc gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa
    13561 tatcgtcttc ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga
    13621 cccggaaacc atgcaggaag tacagtatcc ggtgctggcc gacttcttca aacctgccct
    13681 gctggcgcgt atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat
    13741 tatcgccgga aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgtagaagt
    13801 ggtcattgaa ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg
    13861 cgcgaggatg ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct cgctgctgct
    13921 gttgcagaaa atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg
    13981 cgcatttacg gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac
    14041 ggttttatga gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg
    14101 acgggcttac ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc
    14161 gccgtcgcca ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt
    14221 cggcgggagt ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg
    14281 tccccgtcag ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga ggcctggacc
    14341 cgcgccacag agcaggaaaa acgccgcgag ggcaacacca cagaactgct ggtgacgcag
    14401 gtgccggggg agcgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca
    14461 cgcccggtgc tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg
    14521 caccctctgg atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc
    14581 agccactggt ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg
    14641 attgatgaaa ttaagcaact gttcggcgca aaaacgctga cggtggatac cggcgcagac
    14701 aatgccgccg gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg
    14761 cgcgatgcct gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa
    14821 tgtctggata tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa
    14881 agatgacaac ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc
    14941 ccacagtcaa ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga
    15001 gagtaaggat tttcgtctgc tggcgcatct gctaagaacc ctgcaacacg ccggtgatcc
    15061 tcatctcgca ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc
    15121 gcagaatatg gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg
    15181 tattgaagtt ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact
    15241 ggcaaaactg gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga
    15301 tgatcttttt gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac
    15361 tccggcggcc tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca
    15421 acccagtgct ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga
    15481 cacgctgtta aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta
    15541 ccgtctgcgc cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga
    15601 tagccgtact ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct
    15661 cggtagtgcc gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta
    15721 ctggctggat ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt
    15781 ggcagacgcc atccacgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg
    15841 gctgctgttt aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc
    15901 ttcgcccgac gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag
    15961 agcctgtttt gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga
    16021 aggcgatccc cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc
    16081 ggggctggta cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat
    16141 gatggtggca gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga
    16201 acaataaaag agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg
    16261 actcaaatca gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct
    16321 gatactggcg tggatttttc tgctggtgtg gatctggtgg aaaggcccga tgtggacgct
    16381 gtatgaagaa cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg
    16441 gattattgcc ctggtgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa
    16501 aatgcaaaag caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca
    16561 gcagcgttat cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt
    16621 cctgtggcag ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact
    16681 gctgcgcgag gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga
    16741 acaacgcctg tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg
    16801 cacactctgc gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct
    16861 cggctggctg aaagaaaagc gcgcgcgcca gccgctgaac gggattattc tgacactcga
    16921 tttacccgat ctgctgaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg
    16981 cagccgctta caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact
    17041 gacccggctt gatttattac agggttttgc cgccctgttc cagtcactca acagacagga
    17101 tcgcgatgcg attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg
    17161 aacagagttg aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga
    17221 tctgatggtc gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca
    17281 gggaagccgt gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa
    17341 cgtgatgctg cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat
    17401 attcacccag tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc
    17461 cctggtggac accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga
    17521 gcctaatctg gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt
    17581 tttctctgcc acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta
    17641 caacggtaac tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt
    17701 gccgcctccg cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt
    17761 acgcgatgcc acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg
    17821 actgtaccag ggacgacgta tcgggcctta tgtg
//
LOCUS       DAJTVK010000085        17449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000085 DAJTVK010000000
VERSION     DAJTVK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17449
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.085"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..502)
                     /locus_tag="OO372_002864"
     CDS             complement(<1..502)
                     /locus_tag="OO372_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6342308.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="OO372_002865"
     tRNA            complement(688..772)
                     /locus_tag="OO372_002865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="OO372_002866"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="OO372_002866"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6342309.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO372_002867"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="OO372_002867"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6342310.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="OO372_002868"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="OO372_002868"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCR6342311.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="OO372_002869"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="OO372_002869"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCR6342312.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="OO372_002870"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="OO372_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCR6342313.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="OO372_002871"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="OO372_002871"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCR6342314.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="OO372_002872"
     CDS             7117..8619
                     /locus_tag="OO372_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6342315.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO372_002873"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="OO372_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6342316.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO372_002874"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="OO372_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6342317.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="OO372_002875"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="OO372_002875"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6342318.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="OO372_002876"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="OO372_002876"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6342319.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="OO372_002877"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="OO372_002877"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6342320.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="OO372_002878"
     CDS             complement(16186..17382)
                     /locus_tag="OO372_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6342321.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4485 a         4335 c         4453 g         4176 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcca
//
LOCUS       DAJTVK010000086        17295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000086 DAJTVK010000000
VERSION     DAJTVK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.086"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            93..1133
                     /locus_tag="OO372_002879"
     CDS             93..1133
                     /locus_tag="OO372_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6342322.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OO372_002880"
     CDS             complement(1165..2358)
                     /gene="lplT"
                     /locus_tag="OO372_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6342323.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OO372_002881"
     CDS             complement(2351..4510)
                     /gene="aas"
                     /locus_tag="OO372_002881"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6342324.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5096..6127
                     /gene="galR"
                     /locus_tag="OO372_002882"
     CDS             5096..6127
                     /gene="galR"
                     /locus_tag="OO372_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6342325.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OO372_002883"
     CDS             complement(6134..7396)
                     /gene="lysA"
                     /locus_tag="OO372_002883"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6342326.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7518..8453
                     /gene="lysR"
                     /locus_tag="OO372_002884"
     CDS             7518..8453
                     /gene="lysR"
                     /locus_tag="OO372_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6342327.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8440..9087)
                     /locus_tag="OO372_002885"
                     /pseudo
     CDS             complement(8440..9087)
                     /locus_tag="OO372_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OO372_002886"
     CDS             complement(9218..10636)
                     /gene="araE"
                     /locus_tag="OO372_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6342328.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OO372_002887"
     CDS             complement(10951..11712)
                     /gene="kduD"
                     /locus_tag="OO372_002887"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6342329.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OO372_002888"
     CDS             complement(11777..12613)
                     /gene="kduI"
                     /locus_tag="OO372_002888"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6342330.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12900..14081)
                     /locus_tag="OO372_002889"
     CDS             complement(12900..14081)
                     /locus_tag="OO372_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6342331.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14336..15565
                     /locus_tag="OO372_002890"
     CDS             14336..15565
                     /locus_tag="OO372_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6342332.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16025..16657
                     /locus_tag="OO372_002891"
     CDS             16025..16657
                     /locus_tag="OO372_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6342333.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            16991..>17295
                     /locus_tag="OO372_002892"
     CDS             16991..>17295
                     /locus_tag="OO372_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6342334.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4581 a         4511 c         3976 g         4227 t
ORIGIN      
        1 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
       61 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
      121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
      181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
      241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
      301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
      361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
      421 tccgcgaagc gctgcatgac agcctcaagc gcctacacac tgattacctc gatctttatc
      481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
      541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
      601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
      661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
      721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtaag ccagtatgaa ggggtcgaac
      781 tgctggccta ttcgtgccta ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
      841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
      901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggccta gaccctgctc
      961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
     1021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
     1081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
     1141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
     1201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
     1261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
     1321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
     1381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
     1441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
     1501 catacagcgt gacatggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
     1561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
     1621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
     1681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
     1741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
     1801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
     1861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
     1921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
     1981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
     2041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
     2101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
     2161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
     2221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
     2281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
     2341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
     2401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
     2461 cagcaagctc cggtacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
     2521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
     2581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
     2641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
     2701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
     2761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
     2821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
     2881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
     2941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
     3001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
     3061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
     3121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt
     3181 aatgcagcgg gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc
     3241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
     3301 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
     3361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
     3421 gctgctgttt tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt
     3481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
     3541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
     3601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
     3661 ttgcgggaat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
     3721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
     3781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
     3841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
     3901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
     3961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa
     4021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
     4081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
     4141 caaaacccgc gccatcgtag attttcatca gcgagcctgt ggtggtgatg cgtccttcag
     4201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atcgccatag
     4261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
     4321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
     4381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
     4441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
     4501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
     4561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
     4621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
     4681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
     4741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
     4801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
     4861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
     4921 agtcaatcgc aacagcattt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
     4981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccgcccc ttgaaccaac
     5041 gggcgttttc cgtaacactg aaacaatgta agcgtttacc cactaaggta ttttcatggc
     5101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
     5161 taataattca cccaaagcca gcgaagcgtc ccggctggct gtgcatagtg caatggagtc
     5221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
     5281 cggtctggtc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
     5341 acaggtggct tatcacaccg gtaatttttt attgattggc aatggttacc acaacgaaca
     5401 aaaagagcgt caggccattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
     5461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
     5521 gctgatcaac cgtatcctgc ctggcttcga aaaccgttgt attgctctgg acgatcgtta
     5581 cggtgcctgg ctggctacgc gtcatttaat tcaacaaggt catacccgca ttggttatct
     5641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
     5701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggtgaac cagacgaaag
     5761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
     5821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
     5881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
     5941 tccgcgcctg accaccgtgc gttacccaat cgtgacaatg gcgacccagg ctgccgaact
     6001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
     6061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
     6121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
     6181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctgttgt
     6241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
     6301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
     6361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
     6421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
     6481 tcagatcgtt gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
     6541 tccgcacctg agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt
     6601 ccagtttcac agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac
     6661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac
     6721 cgcccgcaga aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac
     6781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
     6841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
     6901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
     6961 ccgggttaac gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca
     7021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
     7081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
     7141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
     7201 gcattaatcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
     7261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
     7321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
     7381 acagtgaatg tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat
     7441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat agattaccgg
     7501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
     7561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
     7621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
     7681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
     7741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
     7801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
     7861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
     7921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
     7981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
     8041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
     8101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
     8161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
     8221 gggcgggggt aggtgtttcg gtggttaacc cactcaccgc actggattat gcggcaagcg
     8281 gtttagtggt acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
     8341 tgcaccgccc gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac
     8401 cgaaactggt cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac
     8461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
     8521 aaccaataaa ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag
     8581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
     8641 ttcttcaaaa ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt
     8701 gatggaaaat tattccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
     8761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
     8821 cactttatgc atcgtattgg tacataacac aataccttct gcgcccgccc gctgtaagcc
     8881 aagcgccgcc tcagccagaa tgtccccggt tttatcccat tccccgcgac gctggcactc
     8941 ttctatttca tgaaaatcga cgctatgtag cagcacttgc gcagagtgaa gcccaccaag
     9001 ccgctgttta atgccttcat ttatcagacg atagtaagga atggtggatt cccagctcat
     9061 tcctcccagc aaaccaattg ttttcattcc tctctctcct gatgtgtgtt accgcaacaa
     9121 ggctgaataa ggaacgagac aaacgcctca acggccaggt gcccaatctc tattaacgaa
     9181 aaaagggccg gatgtacagc acatccggcc cgtgaaatca gacgccgata tttctcaact
     9241 tctcgcctgc catcagtttg cgttcgatat gttccagcgt gacatttttg gtttccggaa
     9301 tgagccagaa agtgatgccc acaaacgcaa tgttcagcgc agtgtagagc cagaacgtac
     9361 cggcagcacc aatgctgtcg agcagcgtca ggaaggtcgc gccgataatc atattcgaca
     9421 cccagttcgt ggtggtcgaa caggtaatac cgaaatcgcg gcatttcagc ggctgaattt
     9481 cagagcacag gatccacacc actggcgcgg cgctcatcgc ataaccggca atacacatca
     9541 tcgtcatgcc aacagagagc caggacaagc cactggaagc cgtaccgtta tcaaactgca
     9601 tcaggcaata gcccagcacc agagtgccta acgccatcac gctgaaacca attttcagag
     9661 ccggtttacg ccctgcttta tctaccgtaa acaccgcaat aaaggtggcg aacataaagg
     9721 tcagccctac gaccagagtc gcaatcatct gttgttctgt ggtcgtaaag cccgccattt
     9781 tgaagatacg cggcgcgtag tacatgatga tgttcatacc ggtaaactgc tgcatcgcct
     9841 gcaacaacat accgagaaac acagcacgac ggacgttacg gttgatctta aacagtgccc
     9901 aaccgccctg ttttaacttc aggctttcac gaatttcgtt gagttcttct cgcgcttttt
     9961 ccgacgtatc gcgcagcata cgcaacactt cttccgcctc aatatgacgc cccttttccg
    10021 ccagccagcg cgggctattt ggcaggaaga ccaccagaat aatcagcagg actgctggta
    10081 aagcaagaac ccccaacatt gcgcgccagt taccgctata actgaacgct gtatcggata
    10141 aaaacgccag cacgatgccg agtgtgacca tcaactggta catactgatc atcttaccgc
    10201 gaacgttttc acttgccatt tcagaaagat acagaggagc ggtgtaagac gcgatcccga
    10261 cagcaatgcc cagcaccacc cgagcggcga ttaacatctc tacgctggtc gcaaaagcgg
    10321 accctataga accgagtaca aacaggatgg cccccgccat caggctgtat ttacgcccca
    10381 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10441 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10501 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10561 aaacaaacat attcatacgc cgcgtatccc gcaaagaacg tggcgttaaa gcagattccg
    10621 tattgatagt aaccattttt tcctgccagc agagagtaag acatagtgaa aaaatacgtg
    10681 aacaactcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10741 tgtcgttatg tgatggatat tccaattttc aaattaagtt gaattattga gattattatt
    10801 aaccacctaa ttttacagca gataaaattc ataaagttca ttaattgata attgatatgg
    10861 attatttcat aaccatgata tgaattatga tgacctacag gtataaaaaa ccctgccatg
    10921 aggcagggtc ataaaagtaa gaggaatgaa ttaacgagcc agccaaccgc catctacggc
    10981 aatggtataa ccattcacat aatctgaagc gctggaggca aggaacacta tcggccccat
    11041 caggtcactc ggcagtcccc aacgaccagc tggaatgcgg tcgagaattt ccgcgctacg
    11101 ttgttcatct gcccgtagtt gttgagtatt gttggtcgcc atgtaacccg gagctatcgc
    11161 attaacatta atgttgtgtt tagcccattc gttcgccatc aatcgcgtca cgcccatcac
    11221 gccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11281 cgtgatattg ataatcttgc cgccattgcg ttgcgcgata aagtgtttcg ccgctgcctg
    11341 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11401 gaactcgaga gcatcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11461 accaaactca gctaccgcgc gatccagcag tgctggaatg ccatcaatct ttcgcagatc
    11521 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgcttgatgg tttcagtcgg
    11581 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11641 ctggcccagt ccagtatcac aaccagtgac gaccgcaact ttaccttcga gagaaaatgc
    11701 acttaaaatc ataacaatac cttattcgtt tatgccgaca actagcgcaa atctttaacg
    11761 gccactatgc cgacaactag cgcaaatctt taacggccac atggtccata tcatcaaaga
    11821 cctggttttc accaaccatg ccccagataa aggtataagc tttggttccg acgccggaat
    11881 ggatcgacca gctgggggag atcaccgcct gctcgttatg catcacaatg tgacgcgttt
    11941 cttgcggctg ccccatcatg tggaaaacgc aggcgtcatc atccatattg aaatagaaat
    12001 aaacttccat ccggcgctcg tgagtgtgac acggcatggt gttccacaag ttacctggag
    12061 ccagctccgt cagccccata ctcaattggc aggtttccag cacatccggg acaaaatatt
    12121 tgttaattgt gcgacggtta ctggtgaggt tatcgcctaa cgtgactgga gatacttcgt
    12181 ccggtgtgac ttttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
    12241 ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttaccaacat
    12301 acagggcgtc gcggtgaccg atttcatagc attggccatc gacagtaatc gtaccggcac
    12361 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
    12421 taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa
    12481 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc ttttcaacca
    12541 aaaattcatt gcgcagccct tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
    12601 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
    12661 aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt
    12721 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
    12781 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc
    12841 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
    12901 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
    12961 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
    13021 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
    13081 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
    13141 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
    13201 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
    13261 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
    13321 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
    13381 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
    13441 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
    13501 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
    13561 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
    13621 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
    13681 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
    13741 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
    13801 gctttaagtc cggaaccgca aacgtcatta atcgtgattg cagaaacgct attaggaaga
    13861 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
    13921 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
    13981 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccacg aaagcagccg
    14041 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
    14101 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
    14161 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
    14221 gcaaccacta acccaccaat agatgggtag ataaggcgtt aatctcccat acttaacctg
    14281 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
    14341 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
    14401 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
    14461 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
    14521 cctctcatca aaaacatcag catgtgaagg gataacgggc gcggtaacac actactatgg
    14581 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
    14641 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
    14701 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaattctga atctcatttt
    14761 tctgatggga cgtcatgcca ctattcgggt aatgggattt ttggtattcc cattgattgc
    14821 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacgac
    14881 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
    14941 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
    15001 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
    15061 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
    15121 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
    15181 agcatggttg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
    15241 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
    15301 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
    15361 gattaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt atgcgatcag
    15421 cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt atctgatccc
    15481 ggcgcttaaa ccctggcgct ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
    15541 cgtatcggta atgttcttta gctaatatcc gttgtggatg gttcaaaatt gctacaattt
    15601 tgaaccattc catttttcat aatattcatt tgttattatt ccgccctatc acttctctcg
    15661 tcatatccta gaattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
    15721 actatttaag atgcataact tagattcgca agatatcttc tggtcattca aaaacaattt
    15781 gcgatataga tattctcata ccatgagata gttatctgga ccacgttcga ccatgcaaga
    15841 ttaaatcagt gaaacataat catatttaat aacgagaaat gcatttttaa atgtcttaat
    15901 cagaattata gcaaatacag attaatcgca atttttccca gcaaaattac aaaatcaaga
    15961 tgtattgaca tcatcaatca ccaatgaaat tatgcaatta tatacggata gggaggttct
    16021 taacatgggg ctttgtagtc gttataaaag tcttacatgc aatagttgct caatgcattg
    16081 ccaaataatg ccagaagagt caccgcgttt gcagtattgc gcaaactcgt gtttttgtat
    16141 gtggcccgaa gaaagttcat attttaatcg tggtgtggtg gaaggtatct taacaaaaaa
    16201 ccacaatgcg agattaagcg gatatatttt tgtggatttt tcagtaagtt ttttacgtct
    16261 attccttgaa aaagactgga ttgactatct tgcaagtact gacatgggga tagttttagt
    16321 cagcgaccgc aatatgcaat cgttagctaa ctactggcga aaacacaaca ccgcaatatc
    16381 tgctgtcata tataatgatg atggtcttga tgttgccaat gaaaagatca gacaactatt
    16441 tattggtcgt tatttatcat ttaccagagg taacacatta actcagatgg aattcaccat
    16501 aatggggtat atggtttctg gttataatcc atatcagatt gctgaagtgc tggatatgga
    16561 tatccgtagc atctacgcgt acaagcaacg aatcgaaaag agaatgggtg gtaaaataaa
    16621 cgaattattt attcgttcac attcggtcca acattgatac tcaacgacca gccagaatca
    16681 tactctgttc atacgggcat ctattgaaat gcccgtattt tataaataag cagtaacccc
    16741 aagagtaaac aacatagtat ttaaaaccat cacacgaaaa attcagaagc attacgaaat
    16801 atgaatttac aaacaatagt ggcataaatg ttaaccatgt taatttacgt aaagttttac
    16861 gttgcaacat taaagcctca tttcaatcat catgataaat ataaaattaa tatatattta
    16921 tgccgtaaat accaatatac ttagcaaact atgtgatctc catttcgatt gatttagtgt
    16981 ttattgacgt atgtactgga ttattaacga taatatcgag ttctggcctg agcaccgaaa
    17041 attaatatcg gtacataacg ccgatcttaa cgtcgttctg acaacgccag ccagtcgatg
    17101 tttatcactt ctacttgaag cttttcctga tgtggttgca caacaagatt ttttcaccag
    17161 agtctgggaa gaagaaggta tgcgtgtgcc tactaacacg ttatatcaga acatatccat
    17221 tatcagacgc ggatttcgcg ctgttggtga tactacccac tcgctaattg caaccgtgcc
    17281 gagagaggat tcaag
//
LOCUS       DAJTVK010000087        17143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000087 DAJTVK010000000
VERSION     DAJTVK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17143
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.087"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO372_002893"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO372_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6342335.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO372_002894"
     CDS             2219..2767
                     /locus_tag="OO372_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6342336.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO372_002895"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO372_002895"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6342337.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO372_002896"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="OO372_002896"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6342338.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO372_002897"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="OO372_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6342339.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO372_002898"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO372_002898"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6342340.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO372_002899"
     CDS             complement(8300..9502)
                     /gene="pncB"
                     /locus_tag="OO372_002899"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6342341.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9768..12380
                     /gene="pepN"
                     /locus_tag="OO372_002900"
     CDS             9768..12380
                     /gene="pepN"
                     /locus_tag="OO372_002900"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6342342.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO372_002901"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO372_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6342343.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO372_002902"
     CDS             complement(13351..14142)
                     /gene="ssuC"
                     /locus_tag="OO372_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCR6342344.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO372_002903"
                     /pseudo
     CDS             complement(14154..15301)
                     /gene="ssuD"
                     /locus_tag="OO372_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO372_002904"
     CDS             complement(15298..16257)
                     /gene="ssuA"
                     /locus_tag="OO372_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6342345.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16250..16825)
                     /gene="ssuE"
                     /locus_tag="OO372_002905"
     CDS             complement(16250..16825)
                     /gene="ssuE"
                     /locus_tag="OO372_002905"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR6342346.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4298 a         4421 c         4350 g         4074 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aaagggagcc
     8281 gattagctcc ctgatgatat taactggctt ttttaatatg cggaaggtcg aacgctttgc
     8341 gcagcgcccg aacaaacgct ttatcatggc agatagtttt gccagggctg tcagaaagtt
     8401 tcgccaccgg tttaccgtta cactctacca acttaatcac aatattcagg ggttttacct
     8461 gggggatatc gcaggtcagg cgagtcccaa taccaaaact taattgcacg cgggaagaga
     8521 agtggcggta tagctcaacc gctttgcgta aatccagatt gtcagagaaa accagcgttt
     8581 tactctgtgg atcaattccc agcttttcat aatgtgcaat ggctttttca ccccattcaa
     8641 ccgggtcgcc agagtcatga cgcaggccct gataccgact agcgaactcg acaccgaaat
     8701 cacgcaggaa agcatccata gtgatgcagt cggttaatgc aatgccaagt tggtcgggat
     8761 actcttccag ccaggcagca agtgcagctc gctggctgtt ggctagatcc gggctgattt
     8821 gctgatgtgc ctggaaccat tcgtgtgcct gtgttcccat cggcgtgagg gaaagccgac
     8881 gcgccagatc gtagttgctg gtgcccacga accaggattc ctgttgcaga cgcttaacga
     8941 tggtttcttg tacttcgcga gaaaaacggc gacgggtgcc aaaatccatc agatggaagc
     9001 gcgacatatc aagaccggcg gttaacgccg agaagtcgac taatttgctt tccagcgtgt
     9061 cgagggcttg cgcaacgtcg gcctgcggtg agcgatagcg atgtaccatt tcactgataa
     9121 ccgccagcaa aggaacttcc cagaggatga cttcacgcca cggaccgctt aagcgaatat
     9181 ccagcttgcc attatcgttg gacacggtga cttgttccgg gttaaagcgg aactcgcgta
     9241 accagttaag atagtcggcc ttaaagaaag gcagggcaga gagccactga tattcatcat
     9301 cctgcaggcg caggtgctgc atcgcctgaa tctgttcacg aatagcatcg gcataaatac
     9361 ccagcagatc gtcacctcgg caacgaaact ccgccgcgac atgcacatcg taatagtgat
     9421 gaaacacggc ttgctgcata tgcaacttat aagcatctgt atccagcaac gagtgcagaa
     9481 caggagaagc gaattgtgtc ataggtgcgc agtagcgtcc tcgtacagga gcgtttagta
     9541 caataaacat cttcaggaac tgctggagta taccttgttt cgcaatttat tgaaccccga
     9601 tcacaccata tgccaccttt ctggtcgatg gcattacgcg gtgtatgtta taaaaatgta
     9661 gcaataaagg cgtttgtacc tgaaaagatg aagattctgc atagcgcgat ttacgcaaca
     9721 ggaatagact gaacaccaga ctctataaaa gatgctaaag gttatttatg actcaacagc
     9781 cacaagccaa ataccgtcac gattatcgtg cgccagatta ccagattact gatattgact
     9841 tgacctttga cctcgacgcg caaaagacgg tcgttaccgc ggtcagccag gctgtccgtc
     9901 atggtgcatc agatgctcca cttcgtctca acggcgaaga cctcaaactg gtttctgttc
     9961 atattaatga tgagccgtgg accgcctgga aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcattttacg cttaagatcg ttaatgaaat aagcccggcg gcgaataccg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaaccaaa tatcccttcc tgctttccaa cggtaaccgc gttgcgcaag
    10261 gtgaactgga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgacaccttt accacgcgtt
    10381 ctggtcgcga agtggcgctg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactcaatga aatgggatga agaacgcttc ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccgattgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggca aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagtga tcagcaactg accttcctga tgcgtcatgc gcgtaatgat ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atcgacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcga aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggaggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctg tacgagaaga
    12361 tcaccaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 caatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctactgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctggccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tgtcttcctg aatctcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc tgccagcagg cgcagcaggg
    13201 tacttttgcc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcataccgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtcc gtttgcaaaa actcccgcgc
    13501 attcatcgcc agataaccaa tgcctgaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcaccagg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatttccac
    13681 cagaccgcga tcgatattac ggatgccatg ccaggtgttg atataaatgg ggaatagcgt
    13741 gccgagcgcc accagaaaga tttttgcgga ttcatcaatg ccaaaccaca aaatcaccag
    13801 cgggatcagc gccagatgcg gtacgttgcg caacatctga attgaggtat ccagcagccg
    13861 ttctccccag cgtgacagcc cgctaatcag tcccagaatc aagcccagcg atccgccaat
    13921 tgaaaagcca atcagcgccc gccaggaact gatcgccaga tgctgccaca attcgccgct
    13981 ggcggagagc gtccagaacg ccgtcaccac tccttccggt gaaggcaaaa tacgcgtcga
    14041 cagccagcca accgaggagg ccagttgcca caccgccacg atgcccaccg gtaaaaacca
    14101 gggggcaacg cgcaataacc acttcttcac tggcgttgcc atcatcgtgt tccttagctt
    14161 tgcgcgactt tacgggggat aaaatcattc gccaccgctt cgccttgcgg attcagcggc
    14221 tgcggctggg gaatttccgg aatggcgaca tccagatgtg ggaacagcaa ctcgccaacc
    14281 cgatacgctt cttccagatg cgggtagccc gaaagcacaa agctgtcgat gccaagcgcg
    14341 gcgtattcgt tgattcgcgc ggcgaccgta ggaccatcgc ccaccagcgc cgttccggca
    14401 ccgccgcgca ctaagccaac gcccgcccat aaattggggc tgatctccag attgtcgcgc
    14461 ttgccgttat gtagcgccgc cattcgctgt tgtcctacgg aatccgtccg ggcgaatgcg
    14521 gcctgtgctt tggcgatagt ttcatcatca agatgcgaga ttaaccgctc ggcggcctgc
    14581 cacgcttcgt cgttagtttc acgaacaatc acatgcagac gaataccgaa acgaattttg
    14641 cgtccatgcg cggcagcttt cgcccgcact tgttcgatct tctcttttac cagctctggc
    14701 ggttcgcccc aggtgaggta gagatcaact tgttctgccg ccagctcctg ggcgacatct
    14761 gacgatccgc caaagtaaag cggaggatac ggctgttgaa tcggcgggaa gagcagtttt
    14821 gctccccgca catgaatatg tttaccgttg aaatcgacgc tttcccccag caataagcgc
    14881 cccgccagac ctgggtaaat tccgccgagg cttcgtagcg ctcgctatga tcaaggaaca
    14941 cgccgtcgcc tgccaactct tgtggatcgc tgcctgtgac cagattaaac aacgcacgac
    15001 catttgagag acggtcaagc gtggcggcct ggcgagcggc aacggtaggt gaggttacgc
    15061 tgggacgcag ggcgacaaga aacttcagcc gctgcgtcac cgggatcatc gacgccgcca
    15121 ccagccacgc atcttcacac gatcgccccg ttgggatcag cacaccggta tagcccagac
    15181 gatccgccgc ttgcgcaatt tgttgcagat agccgtgatc aaccgggcgt gaaccttctt
    15241 ccgttccaag ataatgcccg tcaccatggg tcggtaaaaa ccagaacata ttcagactca
    15301 taattgtttt ccttccaggt gagtgggctg ccagatgcgc tggcgaatat caactttttt
    15361 cgacaccaga cggttttcat aaaacagatc tgccgtttgc tgctgtaagg cggcaacttc
    15421 agcgctcacc ggtttgatgg tggtgggagg acgatgatcc agatacgagg caatcaccgg
    15481 tgccggtaag cccatcgttt ttgccagtaa agcgatgctt tgctcgcgct ggctgcgggt
    15541 taacgcatcg gcctcactaa aggttgccag tacgccctga ataaaagcgc cgtttttttc
    15601 tgcatagggg cgagctgcca gataaaacga tccagtttga ttgagatcgg tgccgtcttt
    15661 cagcacccgc acgccgccct gtaataacgc agcggagtag tagggatccc agatagccca
    15721 ggcgtcaacg ttaccttgct ggaacgcggc gcgggcatca gctggcgtca gataagtggg
    15781 ctggatgtca gtaaacttaa gtccggcctg acgcagtgca cgcagtaaaa ggttgtgtga
    15841 actggaacct ttctgaaagg caactttgtg acctttaaga tcggctacgg ttttgatcgg
    15901 gctgttttct gccaccagaa tcacttcggc tttgggcttc ggtggctcga cgcccacata
    15961 tatcaaatca gccccggcag cctgggcaaa gattggcgga atatccccgg tactgccgag
    16021 atcaatactg ccaacgttta acgcttccaa catttgcgga cccgcgggga actccaccca
    16081 ggagattttt gattgcggat agcgtttttc cagtaactgg tggctttttg ccaataccat
    16141 accaatactg cctttctgat agcctatacg taacgcttca ggcgaggatt ctgcagcaac
    16201 cgcaaaagta gagacgctaa gcaatcccgc cagcgccagt ttaatgatgt tacgcatgga
    16261 cattacctcg cagagacaga aggtcaggaa cctgaacatc gcggcggtgc aatgcctgcc
    16321 agaaagtttc tagcgcggta tcaagacggg tttgcagatt tggcgtgaac tggggtttgt
    16381 gatggtaatc aataacttgt gagtcatcgg caaacacgcc gtgcaggagc tcctgggctt
    16441 tcatcgcact taaaactggc ttaagggcat aatcgaccgc cagcagatga gccaccgtac
    16501 cgcccgtcgc cagcggtagc accactttgc cttgcaaagc gcgctctggc agcaggtcga
    16561 gcagggtttt cagcgcgccg gaataggcgg ctttatacac aggtgtagcg actatcagtc
    16621 catcagcctg ttgcagctgt tcggtgaagg tcttgagtgc cggactatcg aaacgagcgt
    16681 aaagcagatc ttccggggcg aagttttgca gattccagtg ataaacctct acatccaggc
    16741 catttagttt ttcccgcgca tattccagca aggagctgga gcgagaagga aagcgaggac
    16801 tacccgccag ggtgatgaca cgcatactct ctccttataa ccaattgttc tttttttgtt
    16861 aacattgata acaattcggt cagtctgtcg gagagacaag aaaatgccaa atataaattt
    16921 tgtgtatctt tttctgaaaa taaactaaag aaagggcttt atatatcacg catatttatt
    16981 tgttggatag tcattagatg ttgaatcaat gaattactcg ttcagttaat atttaaatat
    17041 tcatgaaatc tataaattaa agatttgtca cttattggat ttagtatgct tgatttaaat
    17101 ggcggtcaat tattgttatt attaagtcta atgtcattta aag
//
LOCUS       DAJTVK010000088        16392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000088 DAJTVK010000000
VERSION     DAJTVK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.088"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="OO372_002906"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="OO372_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR6342347.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="OO372_002907"
     CDS             complement(2362..2802)
                     /locus_tag="OO372_002907"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6342348.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OO372_002908"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="OO372_002908"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6342349.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="OO372_002909"
     CDS             3520..4218
                     /locus_tag="OO372_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6342350.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="OO372_002910"
     CDS             4447..5655
                     /locus_tag="OO372_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6342351.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="OO372_002911"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="OO372_002911"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6342352.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="OO372_002912"
     tRNA            7762..7838
                     /locus_tag="OO372_002912"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
     gene            7873..8006
                     /locus_tag="OO372_002913"
     ncRNA           7873..8006
                     /ncRNA_class="other"
                     /locus_tag="OO372_002913"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8751..10358
                     /gene="dppA"
                     /locus_tag="OO372_002914"
     CDS             8751..10358
                     /gene="dppA"
                     /locus_tag="OO372_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6342353.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10666..11685
                     /gene="dppB"
                     /locus_tag="OO372_002915"
     CDS             10666..11685
                     /gene="dppB"
                     /locus_tag="OO372_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6342354.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11695..12597
                     /gene="dppC"
                     /locus_tag="OO372_002916"
     CDS             11695..12597
                     /gene="dppC"
                     /locus_tag="OO372_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6342355.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12608..13591
                     /gene="dppD"
                     /locus_tag="OO372_002917"
     CDS             12608..13591
                     /gene="dppD"
                     /locus_tag="OO372_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6342356.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13588..14592
                     /gene="dppF"
                     /locus_tag="OO372_002918"
     CDS             13588..14592
                     /gene="dppF"
                     /locus_tag="OO372_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6342357.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14622..15893)
                     /locus_tag="OO372_002919"
     CDS             complement(14622..15893)
                     /locus_tag="OO372_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6342358.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3898 a         4088 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc ggggaggttc
     7921 gactcgagcg aagcgagaga acgttgcgcc agcaacggcc cgcagggcga gccacgaagt
     7981 ggcgagtaat cctctatcac cgaccaaatt cgaaaagcct gctcaacgag caggcttttt
     8041 tgcatttgta gctcatgagg atgagaacct ccgggggcag ggaggttcga ctcgacgtag
     8101 gcctgataag acgcgccagc gtcgcatcag gcaacggcac cgcatttatt gtcggatgcg
     8161 gcgcgaccgc cttattcgac caacacacca cacattttcc tgccaacttt gcgccccata
     8221 tctcactatc cattaacgga tttgtgacag aaaccattgt ctttttttat atttgcttaa
     8281 atcttttttt gcgttactta tggctggcgc tggcattttc cgctgtgcgt tttagcgttc
     8341 atggtattaa tcgctcagat atccaccttt tcctcagatt tttttcatta aatgcataaa
     8401 acttaatcac caaatgttgc taaatattaa gctgattgtt ctggttatgt tggagtagca
     8461 caaatttctc tgctgcaaat agctgtttga agttttttta atctttgttt gtggattctc
     8521 accgttggtg taaatcccgg ttggctgtat tgacgttttc acattctgtt gacagattgt
     8581 aggtcacgag gggcatttta tggaggatcc gcactgttac actgatgtta attagtacgg
     8641 catccccacc tcataacgtt gacccgaccg ggcaaaaaac aaaaaaggtc aggcagcgac
     8701 aacccactgc aaagggttaa aacaacaaac atcacaattg gagcagaata atgcgtattt
     8761 ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct ggtggctatg accgtcgcag
     8821 caagtgttca ggctaaaact ctggtttatt gctcagaagg atctccggaa gggtttaacc
     8881 cgcagctgtt tacctccggc accacctatg acgcctcttc cgtaccgctt tataaccgcc
     8941 tggttgaatt taaaatcggc accactgaag tgatcccggg cctcgctgaa aagtgggaag
     9001 tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa aggtgtgaag tggcacgaca
     9061 ataaagaatt caaaccgacg cgtgaactga acgccgatga cgtggtgttc tcgttcgacc
     9121 gtcagaaaaa cgcgcaaaac ccgtaccata aagtctctgg cggcagctac gaatacttcg
     9181 aaggcatggg cttgccggag ctgatcagtg aagtgaaaaa ggtggacgac aacacagttc
     9241 agttcgtact gactcgcccg gaagcgccgt tcctcgctga tctggcaatg gacttcgcct
     9301 ctattctgtc aaaagaatat gctgatgcga tgatgaaagc cggtacaccg gaaaaactgg
     9361 acctcaaccc aatcggaacc ggtccgttcc agttacagca gtaccaaaaa gattcccgta
     9421 tccgctacaa agcgtttgat ggctactggg gcaccaaacc gcagatcgat acgctggttt
     9481 tctctattac ccctgatgct tccgtgcgtt acgcgaaatt gcagaagaat gaatgccagg
     9541 tgatgccgta cccgaacccg gcagacatcg ctcgcatgaa gcaggataaa tccatcaacc
     9601 tgatggaaat gccggggctg aacgtcggct acctctccta taacgtgcag aaaaaaccgc
     9661 tggatgacgt gaaagttcgc caggcactga cctacgcggt gaacaaagac gcgatcatca
     9721 aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct gatcccgcca accatgtggg
     9781 gctataacga cgacgttcag gattacacct acgatcctga aaaagcgaaa gccttgctga
     9841 aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg ggcgatgccg gtacaacgtc
     9901 cgtataaccc gaacgctcgc cgcatggcgg agatgattca ggcagactgg gcgaaagtgg
     9961 gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta cctcaagcgt gcgaaagatg
    10021 gcgaacatca gacggtaatg atgggctgga ctggcgataa cggggacccg gataacttct
    10081 tcgccaccct gttcagctgc gccgcctctg aacaaggttc taactactca aaatggtgct
    10141 acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac cgacgaccat aacaaacgcg
    10201 ttgaactgta caagcaagcg caggtggtga tgcacgatca gactccggca ctgatcatcg
    10261 ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa aggctatgtg gttgatccat
    10321 taggcaaaca tcacttcgaa aacgtctcta tcgaataatt aaaagccata caagactgat
    10381 ggcaaaggca aaactgcctg atgtgctccg cttatcaggc ctacgaaaat tctgcaatgt
    10441 attgaatttg cacgattttg taggccggat aaggcgttaa cgccgcatcc ggcataaaca
    10501 aagcgcactt tgtcaacaat ctgtataccc ggtggcgatg tgcctctgcg tgcagcgcca
    10561 tccggcagca atacttcatt ccctgtccga taagggcggg aattgatttg tgagcaatac
    10621 agacacgcag ttccaggctg cgggtcacta tagagaatcc gggttatgtt gcagtttatt
    10681 ctccgacgtt tgggactcgt catccccacg tttatcggta ttacccttct cacatttgcc
    10741 tttgtccaca tgatcccggg cgatccggtg atgatcatgg caggcgaacg cgggatctcc
    10801 cctgagcgtc acgcgcagct gctggctgaa ctcggcttag ataaaccgat gtggcagcag
    10861 tatctccatt acatttgggg cgtaatgcac ggcgatctag gcatttcaat gaaaagccga
    10921 attccagtat gggaagagtt cgtgccgcgc tttcaggcca cgctggaact tggcgtctgc
    10981 gcgatgattt ttgctacggc agtcggtatt ccggttggtg tgctggctgc ggttaaacgc
    11041 ggttccattt tcgatcacac tgcagttggc ctggcgctga ccggctactc gatgccgatc
    11101 ttctggtggg gcatgatgct gatcatgctg gtttcggtgc actggaacct gacgcccgtc
    11161 tccggtcgcg tgagcgatat ggtgttcctc gatgactcca atccgttaac cggttttatg
    11221 ctgatcgaca ccgccatctg gggtgaagat ggcaacttta tcgacgccgt tgcccatatg
    11281 atcttaccgg ctattgtgct gggtactatc ccgctggcgg ttatcgttcg tatgacgcgc
    11341 tcttcgatgc tggaagtgct gggtgaagat tacatccgca ccgcgcgcgc caaagggttg
    11401 acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg cgatgctgcc ggtagtgacc
    11461 gttatcggcc tgcaggtggg aacattgctg gcgggggcga ttctgaccga aaccatcttc
    11521 tcgtggcccg gtctgggacg ctggttgatt gacgcactgc aacgccgcga ctatccggta
    11581 gtgcagggcg gcgtattgct ggtggcgacg atgattatcc tcgtcaacct gctggtcgat
    11641 ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga agtaagggga catcatgtca
    11701 caggttactg aaaataaagt gattagcgca ccggtgccga tgaccccgtt acaggagttc
    11761 tggcactatt ttaaacgcaa caaaggcgcg gttgtcgggc tggtttacat cgtcatcgtg
    11821 ctgtttatca cgatctttgc caactggatt gcgccttata acccggcgga tcagttccgc
    11881 gatgcgctgc tcgccccgcc agcctggcag gaaggcggca gcatggcgca cttgctgggc
    11941 accgatgacg taggccgtga tgtgctgtcg cggctgatgt acggcgcgcg cttgtcgctg
    12001 ctggttggct gtctggtggt ggtgttatcg ctgattatgg gcgttattct cggcctgatc
    12061 gccggttact tcggcggcct ggtcgataac atcatcatgc gcgtggtcga tatcatgctg
    12121 gcgctgccaa gtctgctgtt ggcactggtg ctggtggcga ttttcggccc gtcgattggt
    12181 aacgccgcgc tggcactgac cttcgttgcc ttgccgcact atgtgcgctt aacccgcgcc
    12241 gccgtgctgg tggaggtgaa tcgcgattac gttaccgcgt ctcgcgtggc aggtgccggg
    12301 gcgatgcgtc agatgtttat taacatcttc ccgaactgcc tggcgccgct gattgttcag
    12361 gcgtcgctcg gtttctctaa cgccattctc gatatggccg cccttggctt cctcggcatg
    12421 ggagcgcagc cgccaacacc agagtggggc accatgctct ccgacgtgtt gcagttcgcg
    12481 caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga tcctgctgac ggtgctggca
    12541 tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc ccaaactgaa gcagtaagag
    12601 gttcgagatg gcgttattaa atgtagataa attatcggtg catttcggcg acgaaagcgc
    12661 accgttccgc gccgtagacc gcatcagcta cagcgtaaaa cagggcgaag tggtcgggat
    12721 tgtgggtgag tctggctccg gtaagtcggt cagttcactg gcgattatgg ggctgattga
    12781 ttatccgggc cgcgtaatgg cggaaaagct ggtgtttaac ggtcaggatt tgcagcgtat
    12841 atcagaaaaa gagcgccgca acctggtggg tgccgaagtg gcgatgatct tccaggaccc
    12901 gatgaccagc cttaacccgt gctacaccgt gggtttccag atcatggaag cgattaaggt
    12961 gcatcagggc ggtaataaga gcactcgccg tcagcgggcg attgacctgc tgaatcaggt
    13021 cggtattccc gatccggcat cgcgtctgga tgtttacccg catcagcttt ccggcggcat
    13081 gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg ccaaaactgc tgattgccga
    13141 tgagccgacc actgcgctgg acgtgaccat tcaggcgcaa atcatcgaac tgttgctgga
    13201 gctacagcag aaagagaaca tggcgctggt gttaattacc catgatctgg cgctggtggc
    13261 ggaagctgcg cataaaatca ttgtgatgta tgcaggccag gtggtggaaa cgggcgatgc
    13321 gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag gcattgctgc gtgcgctgcc
    13381 agaatttgct caggacaaag aacgtctggc gtcgttgcca ggtgtcgttc ccggcaagta
    13441 cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc tatgccactg acagatgtcg
    13501 cgctgaagaa ccggcgctga atatgctcgc tgacgggcgc cagtccaaat gccattaccc
    13561 acttgatgat gccgggaggc cgacactatg agtacgcaag aggccaccct gcaacaaccg
    13621 ctgttgcagg ctatcgacct gaaaaaacat tatccggtga agaaaggcat gttcgcgccg
    13681 gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc ttgaacgtgg caaaacgctg
    13741 gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg gtcggttgct gacgatgatt
    13801 gaaacgccca ccggtggcga gctgtattac caggggcagg atctgcttaa gcacgatccg
    13861 caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct tccagaaccc ttacggttcg
    13921 ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc cgctgctgat caacaccagc
    13981 ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga tggcgaaagt cggcctgaaa
    14041 accgagcact atgaccgcta tccgcatatg ttctccggcg gtcagcgtca gcgtatcgcc
    14101 atcgcccgtg gtctgatgct cgacccggat gtggtgattg ccgatgaacc ggtttccgcg
    14161 ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga tggatttgca gcaggagttg
    14221 gggctgtctt atgtctttat ctcccacgac ctgtcggtgg tggagcacat tgctgatgaa
    14281 gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa cgaaagacca aatcttcaat
    14341 aacccgcgcc atccgtacac tcaggcgcta ctttccgcga cgccgcgcct gaacccggac
    14401 gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa gcccactgaa tccaccgccg
    14461 ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc cctgcaccca gttgcagccg
    14521 cagctaaaag actacggcgg tcaactggta gcttgttttg ctgttgatca ggatgaaaat
    14581 ccgcagcgtt aactcacaaa accggggcca tgcgctccgg tttactcaat cagcttcaac
    14641 agtggcgaca agcatagcaa aatgccgtac agcagaatca gccaggcttt aaatccgcga
    14701 agtttttcca gttgtgcgac tttatagatc aggaaaaacg gaatgaggca cgacacaata
    14761 ccatataacg ggcttcccaa ctgaaagaac accagcaccg atacacgaaa cgaaacccaa
    14821 atcgtcaacg taatgacgat aaaagcgcag atcgccagag tcagcacgcg tgagttaatt
    14881 ttcttggtat caataattcg gcttaacaga ttgagaataa tgcctttaat ggcctcgtgg
    14941 aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa agatattaag caccgtagag
    15001 gtgatatgaa tgatatgccc agggatcacc tgcgcggcca gcgccagtgc tgagatattt
    15061 tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga aggtaaacga aaaggcaaaa
    15121 aacaggatca ccgcgatgag cgtgatataa ctaattcggt gggtacgcag cgcgagccgg
    15181 gttgccagta ctttatccgc ttcccgttta cgataggcaa tattcattgg gttaagtacc
    15241 tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa gcaagacatc gcggaaaaag
    15301 acggaggctt gcgggaaggc ggttatattg gcgaaattcc agtgcgggat catcgcaaaa
    15361 ccgaacacga caataatccc tactttgacc accaccattg gcccggaaat cttaaacagt
    15421 aatcgttcac caccagacgc aatcgctacc agtacggcga aaatagcgac tttataaagt
    15481 agagattgtg aaagatcggc atcggttaaa ccgaaggttt tcaggtacga ggcgctgtcg
    15541 aaaaccacgg agagagagta gataaaaatc ccgtggataa tcatcaaaaa gtagataacc
    15601 ccgaggaaaa ttccccagtt cttccccagg taatgactga taatatcggt gtagtcatta
    15661 caggaatcgc tttcagaaag ggtttttaaa taaatgtcct gcactaccca ggtggcagga
    15721 taagcaatga tcgctgcggt aataaatacc caaattccct tcaagccaat ttgtactggc
    15781 atcagcacgg ttccggcacc aatcgccatg cctatgcata ataaaaccca gccgaagtcg
    15841 tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg tgttgtgctg cataaagtcc
    15901 tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc agtttctctt gcgcaacagg
    15961 tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac agattcactg gggaggtcgc
    16021 gaggaggcgc aattctacaa aattgcgcat ttcactacca gtctggagtc agcccggata
    16081 tgaactgcaa ctattgcaaa taaagaaatt aagctggaag gcatcgcgca atgaggaaga
    16141 ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc ggcaagccga aagggttccg
    16201 gtgaggcgca atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa
    16261 cccccgcacg ttgcaggtat gtacctgaca acataacggg ggctaatctt gaacctagac
    16321 cactcaagaa tagccgcgaa acgttgtcat tacaacacag gcggctatat gacgctcgca
    16381 gagctgggca tg
//
LOCUS       DAJTVK010000089        16296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000089 DAJTVK010000000
VERSION     DAJTVK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.089"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..578
                     /locus_tag="OO372_002920"
     CDS             <1..578
                     /locus_tag="OO372_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6342359.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="OO372_002921"
     CDS             581..1744
                     /locus_tag="OO372_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6342360.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="OO372_002922"
     CDS             1828..3453
                     /locus_tag="OO372_002922"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6342361.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OO372_002923"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="OO372_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6342362.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OO372_002924"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="OO372_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6342363.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="OO372_002925"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="OO372_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6342364.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OO372_002926"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="OO372_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6342365.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OO372_002927"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="OO372_002927"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6342366.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="OO372_002928"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="OO372_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="OO372_002929"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="OO372_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6342367.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9726
                     /gene="ulaC"
                     /locus_tag="OO372_002930"
     CDS             9262..9726
                     /gene="ulaC"
                     /locus_tag="OO372_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6342368.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9740..10390
                     /gene="ulaD"
                     /locus_tag="OO372_002931"
     CDS             9740..10390
                     /gene="ulaD"
                     /locus_tag="OO372_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6342369.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10400..11254
                     /gene="ulaE"
                     /locus_tag="OO372_002932"
     CDS             10400..11254
                     /gene="ulaE"
                     /locus_tag="OO372_002932"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6342370.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11254..11940
                     /gene="ulaF"
                     /locus_tag="OO372_002933"
     CDS             11254..11940
                     /gene="ulaF"
                     /locus_tag="OO372_002933"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6342371.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12037..12588
                     /locus_tag="OO372_002934"
     CDS             12037..12588
                     /locus_tag="OO372_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR6342372.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12663..12978)
                     /locus_tag="OO372_002935"
                     /pseudo
     CDS             complement(12663..12978)
                     /locus_tag="OO372_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13305..13700
                     /gene="rpsF"
                     /locus_tag="OO372_002936"
     CDS             13305..13700
                     /gene="rpsF"
                     /locus_tag="OO372_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6342373.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13707..14021
                     /gene="priB"
                     /locus_tag="OO372_002937"
     CDS             13707..14021
                     /gene="priB"
                     /locus_tag="OO372_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6342374.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14026..14253
                     /gene="rpsR"
                     /locus_tag="OO372_002938"
     CDS             14026..14253
                     /gene="rpsR"
                     /locus_tag="OO372_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6342375.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14295..14744
                     /gene="rplI"
                     /locus_tag="OO372_002939"
     CDS             14295..14744
                     /gene="rplI"
                     /locus_tag="OO372_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6342376.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14856..15827)
                     /locus_tag="OO372_002940"
     CDS             complement(14856..15827)
                     /locus_tag="OO372_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCR6342377.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15880..>16296)
                     /locus_tag="OO372_002941"
     CDS             complement(15880..>16296)
                     /locus_tag="OO372_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6342378.1"
                     /translation="SWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDM
                     TPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPIN
                     KIIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3826 a         3981 c         4334 g         4155 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tcctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgagcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9421 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9481 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9541 gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc caacacgcac
     9601 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
     9661 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
     9721 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
     9781 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
     9841 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
     9901 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
     9961 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10021 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10081 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10141 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10201 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10261 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10321 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10381 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10441 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10501 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10561 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10621 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    10681 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    10741 ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc gtcgtcgttt
    10801 ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga cgctggcgat
    10861 ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt acgcgcacta
    10921 tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg cgtgggacaa
    10981 cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtgc atgtgaaaga
    11041 caccaaaccg ggcgtcttca aaaacgtgcc atttggcgaa ggtgtggtgg atttcgaacg
    11101 ttgcttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg agatgtggag
    11161 cgaaacggcg gaagacccgg cggcagaagt ggtgaaagcg cgtgattggg tgaaagcgcg
    11221 catggccaaa gcaggcacgg tggaggcggc ataatgcaaa agctaaaaca gcaggtgttt
    11281 gaagccaaca tggatctgcc gcgctatggg ctggtgacct ttacctgggg caacgtcagc
    11341 gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgttgc ctacgaaacc
    11401 atgaaagcag acgatatggt ggtggtcgat atgagcggca aggtggtgga aggggagtat
    11461 cgcccgtctt ccgacaccgc gacgtatctc gaactctacc gtcgttaccc gtcgcttggc
    11521 ggcattgtcc atacccactc cactcatgct actgcatggg cgcaggcggg gctggcgatc
    11581 ccggcgttag gcactacgca cgccgactac ttctttggcg acatcccgtg tacgcgcgga
    11641 ttaagcgaag aagaggtgca gggcgagtat gaactgaaca ccggcaaagt gattatcgaa
    11701 acgctgggtg acgccgagcc gctgcatacg ccggggattg tggtgtatca gcacggtccg
    11761 ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtggtgat ggaagaagtg
    11821 gcgaaaatgg cgtggattgc gcgcagtatt aacccacaac tgaatcacat cgacagcttc
    11881 ctcatgaata aacacttcat gcgtaagcac ggtcctaacg cctattacgg gcagaagtaa
    11941 gcagtgcgcc ccggaattat tacctcctgc gggatgtaat tggtctgatt ccggggccgc
    12001 ttcagttaaa aaatcgtata aacaacaggt gtttttatgg ctatcaatgg attgtttgtc
    12061 agaacggatc cgcaacgtcg ccgctatggc gttgcgttac ttgtgggtgt actttcagga
    12121 attatttcag catttgttaa atggggagcg gaagtcccct tgccaccgcg cacattttcc
    12181 ggtggtcgcg atgaatttaa cccgccgtat atctttctac gggattattt aggtatcgac
    12241 ccaacccaaa ctgtgtatac attctcggaa catattatca acccggtgat ggtgacgcat
    12301 atcatcttct cactggtttt tgcgataggg tattgtattg tcgcggaaat tttcccgaaa
    12361 gtgaaattat ggcaggggat attagcaggg cttattgtga cagtggcagt ccacggtata
    12421 ttttgcccgg cgctgaattt aacgccgccg ctcactcaat tgccttttga cgaatatgcc
    12481 tctgaaatcc tggggcatat tttctggttc tggataatag aaattatgcg ccgcgactta
    12541 cgtaatcgca ttactcatga gccagatccg gaagtcgcaa tccggtgaat aaccgcataa
    12601 aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat gtttcgttgg ttatacctgg
    12661 tgctagcgat aaatatccgc gctggcgtgc atattgccgt tactccccgg ttcccgcatc
    12721 agaattacgt ggtagtacgt tactccccgg ttcccgcatc agaattacgt ggtagtacgt
    12781 tgcgccctgc gcatctgtct cttcatttaa tgcctgacgc gcttcgcttt cagtggcgaa
    12841 attatgattg atataaatca cgcctaagct ttgcacatca tccatgtttc tggcctggtg
    12901 gttattaatt tcaatggctg cccatgtatt tgcacttagc aaaagcacag ccagaagggc
    12961 taaaactcga ctgaacatag atacctcctc gacggctgac tttgtgtgct ctcctctgtg
    13021 atgatcttct gatttaattt taatcaatga taaagaagtt gatggtgacc atttctgatg
    13081 cagttgttca aaaaaacacc atgatgaagt gtgatgaact ccaaatcagc ggtttagagg
    13141 ttaattgcga aaggggagat ttatttcggc tctgcccttg agtttagcgg ggcatacaag
    13201 tactataacg cgtcattttt cagccgacct ttaacacgtt ccttgcctcc ccgggattcg
    13261 gctgacccag acaggaggct gaataatccg taaggagcaa ttcgatgcgt cattacgaaa
    13321 tcgtttttat ggtccatcct gaccagagcg aacaggttcc gggcatgatc gagcgctaca
    13381 ctgctgccat cactggtgca gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc
    13441 agctggctta cccgatcaac aaactgcaca aagcacacta cgttctgatg aacgttgaag
    13501 ctccgcagga agtgatcgat gagctggaaa ctaccttccg cttcaacgat gccgttatcc
    13561 gcagcatggt tatgcgtacc aagcacgctg ttaccgaagc atctccgatg gttaaagcga
    13621 aagacgagcg ccgtgagcgt cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag
    13681 ctggggattc tgaagagtaa tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt
    13741 gcaggactcc ccttcgaaag gtcagtccat caggaattcc tcactgccag ttcgtgcttg
    13801 agcatcgttc tgtgcaggag gaagccggtt ttcaccggca ggcgtggtgc caaatgcccg
    13861 ttattgttag cggacacgaa aaccaggcca ttactcacag tataacggtc ggcagtcgca
    13921 taaccgttca ggggttcatt tcatgccaca aggcaaagaa cggactgagc aaaatggttt
    13981 tgcatgccga gcagattgaa ttgatagatt ctggagacta gccatatggc acgttatttc
    14041 cgtcgtcgca agttctgccg tttcaccgcg gaaggcgttc aagagatcga ctataaagat
    14101 atcgctacgc tgaaaaacta catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc
    14161 ggtacccgtg caaaatacca gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg
    14221 tccctgctgc cgtacactga tcgccatcag taatcggtca cggtccatta atacgacttt
    14281 gagaggataa ggtaatgcaa gttattctgc ttgataaagt agcaaacctg ggtagcctgg
    14341 gtgatcaggt aaacgttaaa gcgggctatg ctcgtaactt cctggtaccg cagggtaaag
    14401 ctgttccagc taccaagaaa aacattgaat tcttcgaagc acgtcgcgct gaactggaag
    14461 ctaaactggc tgaagttctg gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg
    14521 aaactgttac catcgcgtct aaagctggcg acgaaggtaa actgttcggt tccatcggta
    14581 ctcgcgacat cgctgacgct gtaactgcag ctggcgttga agtggctaag agcgaagttc
    14641 gtctgccgaa cggcgttctg cgtaccactg gcgagcacga agtgagcttc caggttcaca
    14701 gcgaagtatt cgcgaaagtg atcgtaaacg tagtagctga ataattcgtt attcaacgag
    14761 acgtaaaaac gccgaccagt ggtcggcgtt ttgcttttgt agcgagcact tgcagatatc
    14821 gataagggcc acaataagca gccctacgaa gataattact tagctgcctg aatatcagcc
    14881 agcattgggg caagggctgg gttgtcctta gcgaagtttg cgtacattgg ctgtactttc
    14941 tcgataaacg gagctttatc tacctggtag actttcacgc ccattttatc cagttttgca
    15001 aattcttgtt tctcttcctc gctccacaag tctttcatgt agctcattga ttctttagct
    15061 gcttttaata taactgcctg attttccggg ctaagtttat caaacgcagc attgctcatc
    15121 agaaccacat cgggaaccat agtgtgttcg tcaaggctga aggattttgc cacttcaccg
    15181 tgacgcatgg tggtcagcgc catcacgctg ttttctgcca tatcgaccac accctgctgt
    15241 aaggctgtat acaactcgcc gtaatccagt ggcgtcgggt taccgcccat gactttgatc
    15301 atttcaactg cgccagggct tggctggaca cggactttca tgcctttcag atcgtctggt
    15361 ttcagtactg gtttattgcc atagaaactg cgggcgcctc catcataaaa agtcagcccg
    15421 aaataacctt tgcttttcgt tgagtcgagg atttttctcc cgatatcgcc ctgcatgacc
    15481 tgataataat gatcacgatc gcggaacaaa taaggcaggc taaataattt ataggaattt
    15541 tcaaatgctt ccatttctgc cgcattggtt tttaccagtg gaatagcgcc agaacgaatc
    15601 agctccattg tttctcgttg agtacccaat gttccatttg gataaatacg aatagtaata
    15661 tcaccgttag agtactcttt gttctttttc gcaaagaact ccatcgcttt atgaacagga
    15721 tgagacttat cctgattatg actcagtttt aatgtcactg cccaggactg gcttgcggat
    15781 aacataagaa cagcaccgat aatgccagcg gatactttta ttttcataga tgttcctttt
    15841 ctaataaata tggcggcagg tgccgccagt gaatgcgcat cagtagccca gtgcttttgg
    15901 caatgccata ctgatttgcg gaatataggt caccattgcc agcaccaata ccatcacggc
    15961 atagaatggc agcataggtt tgataatttt attaatcggt aatttagata cactacaacc
    16021 gacaaataac acactgccta caggcggagt acaaataccg atacacatgt tcagtaccat
    16081 gacaatacca aagtgaatcg ggtccatgcc cagttcggtg acaacaggta aaaatatcgg
    16141 cgtaaaaatc agtaccgccg gtgtcatatc cataaaagtg ccgataatca acaggataat
    16201 attgataata agcaggatta ttattgggtt gtcagaaata gtgttaagca aatcgagaat
    16261 caggaaggga acgtcagcgt ttgacattgc ccatga
//
LOCUS       DAJTVK010000090        16181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000090 DAJTVK010000000
VERSION     DAJTVK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.090"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO372_002942"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="OO372_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6342379.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="OO372_002943"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="OO372_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6342380.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIEGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="OO372_002944"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="OO372_002944"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6342381.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="OO372_002945"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="OO372_002945"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6342382.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="OO372_002946"
     CDS             complement(5254..6294)
                     /locus_tag="OO372_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6342383.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="OO372_002947"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="OO372_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6342384.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="OO372_002948"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="OO372_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6342385.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="OO372_002949"
     CDS             7229..7669
                     /locus_tag="OO372_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6342386.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="OO372_002950"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="OO372_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6342387.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="OO372_002951"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="OO372_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6342388.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..10832
                     /gene="rsxC"
                     /locus_tag="OO372_002952"
     CDS             8898..10832
                     /gene="rsxC"
                     /locus_tag="OO372_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6342389.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10833..11891
                     /gene="rsxD"
                     /locus_tag="OO372_002953"
     CDS             10833..11891
                     /gene="rsxD"
                     /locus_tag="OO372_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6342390.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11895..12515
                     /gene="rsxG"
                     /locus_tag="OO372_002954"
     CDS             11895..12515
                     /gene="rsxG"
                     /locus_tag="OO372_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6342391.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12519..13214
                     /gene="rsxE"
                     /locus_tag="OO372_002955"
     CDS             12519..13214
                     /gene="rsxE"
                     /locus_tag="OO372_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6342392.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13214..13849
                     /gene="nth"
                     /locus_tag="OO372_002956"
     CDS             13214..13849
                     /gene="nth"
                     /locus_tag="OO372_002956"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6342393.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14460..15962
                     /gene="dtpA"
                     /locus_tag="OO372_002957"
     CDS             14460..15962
                     /gene="dtpA"
                     /locus_tag="OO372_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6342394.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16067..>16181
                     /locus_tag="OO372_002958"
     CDS             16067..>16181
                     /locus_tag="OO372_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6342395.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
BASE COUNT         3761 a         4116 c         4125 g         4179 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat tgaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccgcta
    10681 ttgcccgtgc caaagcgcgc aagctggaac agcaacagac taacgcagaa ccagaagaac
    10741 aggttgatcc gcgcaaagcg gcagttgccg cggctattgc ccgcgctcag gccaaaaaag
    10801 ccgcccagca gaaggttgta aacgaggact aaatggtatt cagaatagct agctcccctt
    10861 atacccataa ccagcgccag acatcgcgca ttatgctgtt ggtgttgctc gcagccgtgc
    10921 caggaatcgc agcgcaactg tggttttttg gttggggtac tctcgttcag atcctgttgg
    10981 catcggttag tactctgtta gccgaagctc tcgtactcaa actacgcaag cagtcggtag
    11041 ccgcaacgtt gaaagataac tcagcattgc tgacaggctt attgctggcg gtaagtattc
    11101 cccccctcgc gccatggtgg atggtcgtgc tgggtacggt gtttgcggtg atcatcgcta
    11161 aacagttgta tggcggtctg gggcaaaacc cgtttaatcc ggcaatgatt ggttatgtgg
    11221 tcttactgat ctccttcccg gtgcagatga ccagctggtt accgccacat gaaattgcgg
    11281 tcaacatccc tggttttatc gacgccatac aggttatttt cagcgggcat accgccagtg
    11341 gtggtgatat gaacacacta cgcttaggta ttgatggcat tagtcaggcg acaccgctgg
    11401 atacatttaa aacctctgtc cgtgccggtc attcggttga acagattatg caatatccga
    11461 tctacagcgg tattctggcg ggcgctggtt ggcaatgggt aaatctcgcc tggctggctg
    11521 gcggcctgtg gttgctatgg cagaaagcga ttcgctggca tattcccctc agcttcttag
    11581 taacgctggc gttatgcgca acgttgggct ggttgttctc accagaaaca ctggcagcac
    11641 cgcaaattca tctgctgtct ggtgcgacca tgctcggcgc attctttatt ttgactgacc
    11701 cggttaccgc ttctacgacc aatcgtggtc gtcttatttt cggcgcgctt gcgggcttat
    11761 tagtctggtt gatccgcagt ttcggcggct atcctgacgg cgtggctttt gccgtcctgc
    11821 tggcgaacat cacggttcct ctgatcgatt actacacgcg tccgcgcgtc tacggccatc
    11881 gcaaagggta aaccatgctg aaaactatcc gaaaacacgg cattacgtta gcgctatttg
    11941 cagcgggttc aacagggtta actgcggcca tcaaccagat gaccaaaacg acgattgctg
    12001 aacaggccag tctgcaacaa aaggcattat ttgatcaggt gctgccagcc gaacgctata
    12061 acaatacgct ggcacagagt tgctatctgg taactgcgcc agagttaggt aaaggtgagc
    12121 atcgggttta catcgccaaa caggatgaca aaccggtagc cgccgttctg gaagcaaccg
    12181 cgccagatgg ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg
    12241 tacttggcac gcgcgtgaca gagcaccacg aaacgccagg gcttggcgat aaaatcgaac
    12301 tgcgcctttc tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg
    12361 cgcactgggc ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta
    12421 ctccccgcgc ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg
    12481 cacaactttc tcaacttcct gcctgtggag aataaagcgt gagcgaaatt aaagacgtta
    12541 ttgttcaggg gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtcctc
    12601 tgttggcggt cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg
    12661 tactgacgct gaccaacctg accatttcga cgctgcgtca ctggacgcca gccgagatcc
    12721 gcattcccat ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca
    12781 acgcctacgc ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta
    12841 actgtatcgt tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg
    12901 cactggacgg cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac
    12961 tacgcgaaat tatcggcaat ggcacattgt ttgacggtgc agatgcgctg ttaggtagct
    13021 gggcaaaagt attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc
    13081 tgccaccagg tgcatttatt ggcctgggac tgatgctggc aggaaaatac ctgattgatg
    13141 aaagaatgaa aaagcgccgt actgaagcag ctgccgaacg tgcattgcca aacggtgaaa
    13201 cagggaatgt ctgatgaaca aagcaaaacg cctggagatc ctcactcgcc tgcgtgagaa
    13261 caatcctcat cccaccaccg agcttaattt cagttcgcct tttgaattgc tgattgccgt
    13321 actgctttcc gctcaggcga ccgatgtcag tgttaataag gcgacggcga aactttaccc
    13381 ggtggcgaat acgcctgcag cgatgcttga actgggcgtt gaaggggtta aaacctatat
    13441 caaaacgatt gggctttata acagcaaagc agaaaatatc atcaaaacct gccgtatctt
    13501 actggagcag cataatggcg aggttccgga agatcgtgct gcgcttgaag ccctgcccgg
    13561 cgtaggtcgt aaaacagcca acgtcgtatt aaacactgca ttcggctggc cgactattgc
    13621 tgtcgacacg cacattttcc gcgtttgtaa tcgtactcaa tttgcgccgg ggaaaaacgt
    13681 cgaacaggta gaagaaaagc tactgaaagt ggttccggca gagtttaaag tcgactgcca
    13741 ccattggttg atcctgcacg ggcgttatac ctgcattgcc cgcaagcccc gctgtggctc
    13801 ttgtattatt gaagatcttt gtgaatacaa agagaaagtt gacatctgaa gaaaaggggt
    13861 aacaccgatt accccattga taacctttct ttatcctctt ttaaaacatc tttttaacgt
    13921 gaatgatgcc attgcttagc gttatcatca ggtaatccgt ttgcggataa ccaaaaatgc
    13981 aggttaattg tttttttaat agcgaaattt tctattcatt cgtgatcaag atcacgatgc
    14041 tgtcgcaatg taaaaacatt gcaagaatca ggttaaaata atttaaattt acacacgcaa
    14101 caaatattga cctacaaaac attacactgg ctatttttca gaaactggac tatctcacta
    14161 gtaaaaacgc taaatagcag aacatatcgc cgttaagcca tcacccccct ctcagtgcag
    14221 tgaaaaaatc tgccattacg ttttttgaaa aatttaacgc tggataacat ttcccggaat
    14281 ggttgaattc cccgcctcag ttatatgtaa cagattatta caaaggactt gtctgaaagt
    14341 gcaagatagt gaacattacc tgccgtttcc cctcccacta taacaattgc gcgtatgttt
    14401 tttagacata acgcgagaaa gcacccccgt taatatggga tgtaaaaaaa gaggtaaaag
    14461 tgtccactgc aaaccaaaaa ccaactgaaa gcgtcagttt gaacgctttc aaacaaccga
    14521 aggcgttcta tctcatcttc tcgattgagt tatgggaacg ttttggttat tacggcctac
    14581 aaggaattat ggctgtttac ctggttaaac aactgggtat gtctgaagcg gattcaatca
    14641 cccttttctc ttcctttagt gccctggttt atggtctggt cgctatcggc ggctggttag
    14701 gtgacaaggt actgggtact aaacgcgtaa ttatgctcgg tgctattgtg ctggcgattg
    14761 gttatgctct ggttgcctgg tctggtcacg acgccggtat cgtttatatg ggtatggcgg
    14821 ctattgcggt cggtaacggc ctgtttaaag ctaacccatc ttctctgctt tctacatgct
    14881 atgagaaaaa cgacccgcgt ctggacggtg cattcaccat gtactacatg tccgtcaaca
    14941 tcggctcttt cttctctatg attgctacgc cgtggctggc cgcgaaatac ggctggagtg
    15001 ttgcgtttgc gttgagcgtt gtaggcctgc tgatcactat cgttaacttc gccttctgcc
    15061 aacgctgggt taaacagtac ggttcaaaac cagacttcga gcctatcaac taccgtaacc
    15121 tgctgctgac cattattggt gttgtggcac tgatcgctat cgccacctgg ctgctgcaca
    15181 atcaggaagt tgcgcgtatg gcgctgggcg ttgttgcctt cggtatcgtg gttatcttcg
    15241 gtaaagaagc cttcgcgatg aaaggtgctg cgcgtcgtaa aatgatcgtt gccttcatcc
    15301 tgatgctcga agccattatc ttcttcgtgc tgtacagcca gatgccaacg tcactgaact
    15361 tctttgcgat tcgtaacgtt gagcactcca ttctgggtct ggccgtagaa cctgagcagt
    15421 atcaggcact gaacccgttc tggatcatca tcggtagtcc gattctggcc gctatctata
    15481 acaagatggg cgataccctg ccgatgccaa ccaagtttgc aatcggcatg gtgatgtgtt
    15541 ctggtgcgtt cctgattctg ccgctgggtg cgaaattcgc gtctgacgct ggtatcgtgt
    15601 ctgtaagctg gctggtcgca agctatggcc tgcagagcat cggggaactg atgatctctg
    15661 gtctgggtct ggcaatggtt gcacaactcg ttccgcagcg tctgatgggc ttcattatgg
    15721 gtagctggtt cctgaccact gccggtgcaa acctgattgg tggttatgtt gcgggtatga
    15781 tggctgtgcc ggataacgtt accgacccgc tgatgtcact ggaagtttat ggtcgcgtat
    15841 tcttgcagat tggtgtcgct actgccatta ttgcagtact gatgctgctg accgcgccta
    15901 aactgcaccg tatgacgcag gatgacgctg cagacaaagc ggcgaaagct gctgtagcgt
    15961 aaaattcagg gaaactcttt tacaagccgc taacttttcg ttagcggctt ttttttgttc
    16021 agcaacgcac taccatactt taaaccacag acaaaaggag ttaccgatga aattgttcta
    16081 caaaccgggt gcctgctctc tcgcttccca tatcaccctg cgtgagagcg gaaaggattt
    16141 taccctcgtc agtgtggatt taatgaaaaa acgtctcgaa a
//
LOCUS       DAJTVK010000091        16072 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000091 DAJTVK010000000
VERSION     DAJTVK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.091"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..563)
                     /locus_tag="OO372_002959"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="OO372_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(797..2217)
                     /gene="espX1"
                     /locus_tag="OO372_002960"
                     /pseudo
     CDS             complement(797..2217)
                     /gene="espX1"
                     /locus_tag="OO372_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
     gene            complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="OO372_002961"
     CDS             complement(2702..2965)
                     /gene="rpsT"
                     /locus_tag="OO372_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6342396.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            3068..3286
                     /locus_tag="OO372_002962"
     CDS             3068..3286
                     /locus_tag="OO372_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6342397.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            3294..4235
                     /gene="ribF"
                     /locus_tag="OO372_002963"
     CDS             3294..4235
                     /gene="ribF"
                     /locus_tag="OO372_002963"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6342398.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            4278..7094
                     /gene="ileS"
                     /locus_tag="OO372_002964"
     CDS             4278..7094
                     /gene="ileS"
                     /locus_tag="OO372_002964"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6342399.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            7094..7588
                     /gene="lspA"
                     /locus_tag="OO372_002965"
     CDS             7094..7588
                     /gene="lspA"
                     /locus_tag="OO372_002965"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6342400.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            7713..8162
                     /gene="fkpB"
                     /locus_tag="OO372_002966"
     CDS             7713..8162
                     /gene="fkpB"
                     /locus_tag="OO372_002966"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6342401.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            8164..9114
                     /gene="ispH"
                     /locus_tag="OO372_002967"
                     /gene_synonym="lytB"
     CDS             8164..9114
                     /gene="ispH"
                     /locus_tag="OO372_002967"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6342402.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            9180..10094
                     /gene="rihC"
                     /locus_tag="OO372_002968"
     CDS             9180..10094
                     /gene="rihC"
                     /locus_tag="OO372_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6342403.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            10261..11082
                     /gene="dapB"
                     /locus_tag="OO372_002969"
     CDS             10261..11082
                     /gene="dapB"
                     /locus_tag="OO372_002969"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6342404.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
     gene            11538..12686
                     /gene="carA"
                     /locus_tag="OO372_002970"
     CDS             11538..12686
                     /gene="carA"
                     /locus_tag="OO372_002970"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6342405.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            12704..15925
                     /gene="carB"
                     /locus_tag="OO372_002971"
     CDS             12704..15925
                     /gene="carB"
                     /locus_tag="OO372_002971"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6342406.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3921 a         3782 c         4254 g         4115 t
ORIGIN      
        1 tccccccagt agaaaggaat ggagataaac agattaaaac gctcctcttc tttattattt
       61 tcatcataag attggctggc agagaaagta taactaatgt gttgccagtt attggagtaa
      121 ctgaattggt aatctttagc atttccactt cgcccccagt aattccgcca aagcgcactt
      181 aatgatacat ttcccagatt attggataaa ggttgcataa tattggcaga aaggctattt
      241 tttctgccaa tatcataaaa atcatcataa tcactctgat gatagtgttt atcattttcg
      301 taaagatggt cgctgaatgt cctgtaatcc tgcgaagcat aacgccaggc ggccacgcta
      361 aatcgagtgg ctgtttgtac tagatattta ttataggcga cctgatagct cgtaccttcg
      421 tgtctggtat cattatttaa cttactactc gatcgggcag cgtaaaaaga gatggcaccc
      481 agcggtgtat tccaaccatt tcccagagtt atagcattat agttatcgga taaaatcgta
      541 cccgattatg tgatcaggtt gttattattt agattaatgt ttaaattttt taatttcttg
      601 atgtgaagtt gatgaaagtt tgaaaattta atattcttaa ttgttccctt ctttataaga
      661 agagcaaggt gccatcatta gagtgacaga gtaggatgtt tcagagcatg cttcagattg
      721 ttttttattt ctctgcagtg tattaataat catcacagat aatttcattc taaaattacc
      781 tctttctttg agtgttttaa tcctgatgaa ctatattttt tataaggagc atgcactcgt
      841 tgatgctctg aaggtatttt ctcatttttg atgatgtttt gtcgtaatcg tgcataaact
      901 tattaagttc tttatacaca tcattattta gctttacatt atcgcgaact aaagtttgta
      961 ccaatggtat caaaatttcg aaacgggacg gtttgtcatc aatacttgcc agggttttga
     1021 atactcctgt gtatctgtaa cctaagtgct tattgacaag ttgagtggtc catcggccag
     1081 gcataatatc ttcacagcgc aaatttttta tcatggtatt agtgaacgat gttccaaata
     1141 tgttgctttt tttaatgaag aggttgttta tttctccatt tgaaaagatg gtgtttgata
     1201 agtcgctttc aaaacatgat atattggtta atacacaaca aaaataatac ccatatgaaa
     1261 ggttgctctt ttctattgtt atatttgata gcaacatttt atgtatatta atattaagag
     1321 cagtcaaata ctggatcttt tgatcgttaa gttcatacat ctccagtgat ttattatagg
     1381 gtaataaaag tataaattcc tttatattat ctccgcagta agaaaagacg acattaaatt
     1441 tattagtggc catgttttca gttgagcagt aagctgattt ttctattaac cgactgggca
     1501 cgcttttaaa taaagataat tttccacata gttttgaata tgccgaattg ctagttttaa
     1561 agttctgcca cattattccc tggttatcat ctatcgatga ggaggggatt ttattgcatt
     1621 gctcattgta tagctcaggg aaaatatttt ttttaataag ctttttgatt tcctggttaa
     1681 gatgactttt tttcttgtct attgcactgt attttgcgtt ttgtgacgtt tcctgatgat
     1741 cataaaatga ctggagattt ctgcaggcct cataaaatat ttcaggagta tatagctgtg
     1801 ggtatatttc ttggttgaag tttgccttta aaaaattaac gaatttttca tggcattctt
     1861 ggtatatctt aatctcttca gaaagacatt cgcttgttct gtcatcaaaa acatccaatc
     1921 taagtaatgt gatacctttg tgtttaaaaa ataaaattga actgtatgtt ctatgagttt
     1981 acatctataa aaatatgcat ctctcagatg agtatattga aaaaaatgat gaaggactgg
     2041 agatggttta ggcaagttat ggcttctaaa aaagtcatca aacaaaatta agaactctgt
     2101 tttatactcg caaaatactt ttactaactc attttgtctt gttttattta ttattacatc
     2161 atctactata tttgatttat aaagacaatg tggcgaagtt gagataccat ttgtcattat
     2221 cagtccttga agttgatgaa tttttattaa tttaatttat gagtgaatgt gactactatc
     2281 aaaaatagct cattaatttt atgaagtaga atataaatag gtatttaacc tgtgttgatt
     2341 gttgttaatt atttacaaga tctaataagg agatatttat gaatggttaa agtgtatagg
     2401 aatttaattc aaaaatagag tggtcttaag tttaatacct caatgtgtat cacaatatcc
     2461 acattctttg taggggagtc tggagattga gtagatattc ttgttcagaa tgtatcagcc
     2521 gatggttcta cgattcttaa gccacgaaga gttcagaaag tgtaacggca tgtctgcaaa
     2581 agccagcatc ttttgactat ctggcaaccg gtagtgtgtt ctctcacgca tcataaaagc
     2641 agcaggcata aaaaaaaccc gcttgcgcgg gctttttcac aaagcttcag caaattggcg
     2701 attaagccag tttgttgatc tgtgcagtca ggttagcctt atgacgtgca gctttgtttt
     2761 tgtggatcag acctttagca gcctgacggt ccacgatcgg ttgcatttcg ttaaatgctt
     2821 tctgtgcagc agctttgtcg ccagcttcga tagctgcgta tactttcttg atgaaagtac
     2881 gcatcataga gcgacggctt gcgttgtgct tacgagcctt ttcagactga atggcgcgct
     2941 tcttagctga tttgatatta gccaaggtcc aactcccaaa tgtgttctat atggacaatt
     3001 caaaggccga ggaatatgcc cttttagcct tcttttgtca atggatttgt gcaaataagc
     3061 gccgttaatg tgccggcact cgttacgtag tgatggcgca ggattctacc agcttgcggg
     3121 gtgtgaatac agcttttccg cgataaaaat tgcagcaggc ggtcactttc ttcccgtgat
     3181 ttgcgccatg gcaatgaaaa gccacttctt tctgatttcg gtactcaatc gccggttaac
     3241 cttgaccgct gtacaaggta tactcggacg attttcactg ttttgagcca gacatgaagc
     3301 tgatacgcgg catacataat ctcagccagg ccccgcaaga agggtgtgtg ctgactattg
     3361 gtaatttcga cggcgtgcat cgcggtcatc gcgcgctgtt acagggcttg caggaagaag
     3421 ggcgcaagcg caacttaccg gtgatggtga tgctttttga acctcaacca ctggaactgt
     3481 ttgctaccga taaagccccg gcaagactga cccggctgcg ggaaaaactg cgttaccttg
     3541 cagagtgtgg cgttgattac gtgctgtgcg tgcgtttcga caggcgtttc gcggcgttaa
     3601 ccgcgcaaaa tttcatcagc gatcttctgg tgaagcattt gcgcgtaaaa tttcttgccg
     3661 taggggatga tttccgcttt ggcgctggtc gtgaaggcga tttcttgtta ttacagaaag
     3721 ctagcatgga atacggcttc gatatcacta gtacgcaaac tttttgcgaa ggtggcgtgc
     3781 gtattagcag caccgctgtg cgtcaggcgc tggcggatga caatctggct ctggcagaaa
     3841 gtttactggg gcacccgttt gccatctccg ggcgtgtagt ccacggtgat gaattagggc
     3901 gcactatagg tttcccgacg gcgaatgtac cgctacgccg tcaggtttcc ccggtgaaag
     3961 gggtttatgc ggtagaagtg ctgggcctcg gtgaaaagcc gttacccggc gtggcaaaca
     4021 tcggaacacg cccaacggtt gccggtattc gccagcagct ggaagtgcat ttgttagatg
     4081 ttgcaatgga cctttacggt cgccatatac aagtagtgct gcgtaaaaaa atacgcaatg
     4141 agcagcgatt tgcgtcgctg gacgaactga aagcgcagat tgcgcgtgat gaattaaccg
     4201 cccgcgaatt ttttgggcta acaaaaccgg cttaagcctg ttatgtaatc aaaccgaaac
     4261 acggaaccga gaatctgatg agtgactata aatcaaccct gaatttgccg gaaacagggt
     4321 tcccgatgcg tggcgatctc gccaagcgcg aaccgggaat gctggcgcgt tggactgatg
     4381 atgatctgta cggcatcatc cgtgcggcta aaaaaggcaa aaaaaccttc attctgcatg
     4441 atggccctcc ttatgcgaat ggcagcattc atattggtca ctcggttaac aagattctga
     4501 aagacattat cgtgaagtcc aaagggcttt ccggttatga ctcgccgtat gtgcctggct
     4561 gggactgcca cggtctgccg atcgagctga aagtagagca agaatacggt aagccgggtg
     4621 agaaattcac cgccgccgag ttccgcgcca agtgccgcga atacgcggcg acccaggttg
     4681 acggtcaacg caaagacttt atccgtctgg gcgtgctggg cgactggtcg cacccgtacc
     4741 tgaccatgga cttcaaaact gaagccaaca tcatccgcgc gctgggcaaa atcatcggca
     4801 acggtcacct gcacaaaggc gcgaagccag ttcactggtg cgttgactgc cgttctgcgc
     4861 tggcggaagc ggaagttgag tattacgaca aaacttctcc gtccatcgac gttgcttttc
     4921 aggcggtcga tcaggatgca ctgaaagcaa aatttgccgt aagcaacgtt aacggcccaa
     4981 tctcgctggt gatctggacc actacgccgt ggactctgcc tgcgaaccgc gcaatctcta
     5041 ttgcacctga tttcgactat gcgctggtgc agatcgacgg tcaggccgtg attctggcga
     5101 aagatctggt tgaaagcgta atgcagcgta tcggcgtgac cgattacacc attctcggca
     5161 cggtaaaagg tgcggagctt gagttgctgc gctttaccca tccgtttatg ggcttcgacg
     5221 ttccggcaat cctcggcgat cacgttaccc tagatgccgg taccggtgcc gttcacaccg
     5281 cgcctggcca cggcccggac gactatgtga tcggtcagaa atacggcctg gaaaccgcta
     5341 acccggttgg cccggacggc acttatctgc cgggcactta tccgacgctg gatggcgtga
     5401 acgtcttcaa agcgaacgac atcgtcgttg cgctgctgca ggaaaaaggc gcgctgctgc
     5461 acgttgagaa aatgcagcac agctatccgt gctgctggcg tcacaaaacg ccgatcatct
     5521 tccgcgcgac gccgcagtgg ttcgtcagca tggatcagaa aggtctgcgt gcgcagtcac
     5581 tgaaagagat caaaggcgtg cagtggatcc cggactgggg ccaggcgcgt atcgagtcga
     5641 tggttgctaa ccgtcctgac tggtgtatct cccgtcagcg cacctggggc gtaccgatgt
     5701 cactgttcgt gcacaaagac acggaagagc tgcatccgcg tactctcgaa ctaatggaag
     5761 aagtggcaaa acgcgttgaa gttgacggca tccaggcgtg gtgggatctt gatgcgaaag
     5821 agatcctcgg cgatgaagct gatcagtacg tgaaagtgcc ggacacattg gatgtatggt
     5881 ttgactccgg atctacccac tcttctgttg ttgacgtgcg tccggaattt gccggtcacg
     5941 cagcggacat gtatctggaa ggttctgacc agcaccgtgg ttggttcatg tcttccctaa
     6001 tgatctccac cgcgatgaaa ggcaaagcgc cgtatcgtca ggtactgacc cacggcttta
     6061 ccgtggatgg tcagggtcgc aagatgtcta aatccatcgg caataccgtt tcgccgcagg
     6121 atgtgatgaa caaactgggc gcggatattc tgcgtctgtg ggtggcatca accgactaca
     6181 ccggtgaaat ggccgtttct gacgagatcc tgaaacgtgc tgccgatagc tatcgtcgta
     6241 tccgtaacac cgcgcgcttc ctgctggcaa acctgaacgg ttttgatcca gcaaaagata
     6301 tggtgaaacc ggaagagatg gtggtactgg atcgctgggc cgtaggttgt gcgaaagcgg
     6361 cacaggaaga catcctcaag gcgtacgaag catacgattt ccacgaagtg gtacagcgtc
     6421 tgatgcgctt ctgctccgtt gagatgggtt ccttctacct cgacatcatc aaagaccgtc
     6481 agtacaccgc caaagcggac agtgtggcgc gtcgtagctg ccagactgcg ctgtatcaca
     6541 tcgcagaagc gctggtgcgc tggatggcac caatcctctc cttcaccgct gatgaagtgt
     6601 ggggctacct gccgggcgaa cgtgaaaaat acgtcttcac cggtgagtgg tacgaaggcc
     6661 tgtttggcct ggcagacagt gaagcgatga acgatgcgtt ctgggacgag ctgttgaaag
     6721 tgcgtggcga agtgaacaaa gtcattgagc aagcgcgtgc cgacaagaaa gtgggtggct
     6781 cgctggaagc ggcagtaacc ttgtatgcag aaccggaact gtcggcgaaa ctgaccgcgc
     6841 tgggcgatga attacgattt gtcctgttga cctccggcgc taccgttgca gactataacg
     6901 acgcacctgc tgatgctcag cagagcgaag tactcaaagg gctgaaagtc gcgttgagta
     6961 aagccgaagg tgagaagtgc ccacgctgct ggcactacac ccaggatgtc ggcaaggtgg
     7021 cggaacacgc agaaatctgc ggccgctgtg tcagcaacgt cgccggtgac ggtgaaaaac
     7081 gtaagtttgc ctgatgagtc aatcgatctg ttcaacaggg ctacgctggc tgtggctggt
     7141 ggtagtcgtg ctgattatcg atctgggcag caaatacctg atcctccaga actttgctct
     7201 gggggatacg gtcccgctgt tcccgtcgct taatctgcat tatgcgcgta actatggcgc
     7261 ggcgtttagt ttccttgccg atagcggcgg ctggcagcgt tggttctttg ccggtattgc
     7321 gattggtatt agcgtgatcc tggcagtgat gatgtatcgc tcgaaggcca cgcagaagct
     7381 aaacaatatc gcttacgcgc tgattattgg cggcgcgctg ggcaacctgt tcgaccgcct
     7441 gtggcacggc ttcgttgtcg atatgatcga cttctacgtc ggcgactggc acttcgccac
     7501 cttcaacctt gccgatactg ccatctgtgt cggtgcggca ctgattgtgc tggaaggttt
     7561 tttgccttct agagcgaaaa aacaataata aaccctgccg gatgcgatgc tgacgcatct
     7621 tatccggcct acagattgct gcgaaatcgt aggccggata aggcgtttac gccgcatccg
     7681 gcaaaaatcc tcaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     7741 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca
     7801 acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc
     7861 aacacctgtt ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg
     7921 cgtttggcgt gccgtcaccg gacctgattc agtacttttc ccgccgtgaa tttatggatg
     7981 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     8041 ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     8101 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ttggaggcgt
     8161 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     8221 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     8281 gtacataacc gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag
     8341 attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     8401 gtacgtaacg aagcaaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg
     8461 accaaagtgc atatggaagt cgcccgcgcc agccgtcgtg gcgaagagtc gattctcatc
     8521 ggccacgccg ggcatccgga agtggaaggt acaatgggcc agtacagtaa cccggaaggg
     8581 ggaatgtatc tggtcgaatc gccggacgat gtgtggaaac tgacggtcaa aaacgaagag
     8641 aagctctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac
     8701 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     8761 acgactaacc gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg
     8821 gtcggttcga aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc
     8881 aaacgcgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     8941 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca aaatgtggtg
     9001 gcacgtttgc agcagctggg cggtggtgaa gccattccgc tggaaggccg tgaagaaaac
     9061 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     9121 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca
     9181 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     9241 ccgcgatttt tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     9301 cggttgagaa aaccacccgc aacgccctgc aactgctgca tttctggaat gcggagattc
     9361 cgctcgccca gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc
     9421 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccaatcggga
     9481 taccagcgtt tctggcgatt cgtgatgccc tgatgcgtgc accagagcct gttaccctgg
     9541 tggccattgg cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgtaaac
     9601 cgtatattcg tcgtctggtg atcatgggcg gttcttctgg tcgcggcaac tgtacaccaa
     9661 atgccgagtt taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa
     9721 tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     9781 tcgctacttt gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact
     9841 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     9901 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     9961 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
    10021 taaaggtggc attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg
    10081 ctctggcgtc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
    10141 tcactgatat ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
    10201 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
    10261 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
    10321 ttgattcagg cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
    10381 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
    10441 accgtgcaaa gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc
    10501 cgtccggaag gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg
    10561 atcggcacta cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat
    10621 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
    10681 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
    10741 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
    10801 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa
    10861 cgtgtgcctg gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggtgaacat
    10921 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgt
    10981 atgacatttg ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaaatggt
    11041 ctttttgata tgcgagatgt acttaatctc aataatttgt aaccacaaaa tatttgttat
    11101 ggtgcaaaaa taacacattt aatttattga ttataaaggg ctttaatttt tggccctttt
    11161 atttttagtg ttatgttttt aaatattcta taagtgccaa aaattacatg ttttgtcttc
    11221 tatttttgtt gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt
    11281 atttcatgca atcttcttgc tgcgcaagcg ttttccagaa caggttatat gatctttttg
    11341 tcgcttaatg cctgtaaaat atgcatgagc cacaaaataa tataaaaaat accgccatta
    11401 agttgacttt tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa
    11461 gcagatttgc attgatttac gtcatcattg tgaattaata tgcaaataaa gtgagtgaat
    11521 attctctgga gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt
    11581 ttcacggtcg ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt
    11641 caatgaccgg ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc
    11701 ttacttatcc ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg
    11761 tacatgcaca aggtctggtg attcgcgatc tgccgctgat tgccagcaac ttccgtaata
    11821 ccgaagacct ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata
    11881 cccgtaagct gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag
    11941 gcgataaccc ggatgcggcg ttggcgttag aaaaagcccg cgcgttccca ggtctgaatg
    12001 gcatggatct ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct
    12061 ggacgttgac cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg
    12121 tggcttatga ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc
    12181 gcctgaccat cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg
    12241 gcatcttcct ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca
    12301 tccagaaatt cctcgaaacc gatattccgg tattcggcat ctgcctcggt catcagctgc
    12361 tggcgctggc gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc
    12421 atccggttaa agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg
    12481 cggtggatga agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg
    12541 gtacgttaca gggcattcat cgcactgata aaccggcgtt cagcttccag ggtcacccgg
    12601 aagccagccc tgggccacac gacgcagcgc cgttgttcga ccactttatc gagttaattg
    12661 agcagtaccg taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga
    12721 tataaaaagt atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt
    12781 tgactactct ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct
    12841 ggtgaactcc aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat
    12901 cgagccgatt cactgggaag tagtacgcaa gattattgaa aaagagcgcc cggacgcggt
    12961 gctgccaacg atgggcggtc agacggcgct gaactgcgcg ctggagctgg agcgtcaggg
    13021 cgtgttggaa gagttcggcg tgactatgat tggtgcgacc gccgatgcga ttgataaagc
    13081 agaagaccgc cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc
    13141 cggtatcgca cacacgatgg aagaagcgct ggcggttgcc gctgacgtgg gcttcccgtg
    13201 cattattcgc ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgcga
    13261 agagtttgaa gaaatttgcg cccgcggtct ggatctctcc ccaaccaaag agctgctgat
    13321 tgatgagtcg ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga
    13381 caactgcatc atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc ataccggtga
    13441 ctccatcact gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa
    13501 cgcctcgatg gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt
    13561 tgcggtgaac ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg
    13621 ttcttcggcg ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact
    13681 ggcggtgggt tacaccctcg acgaactgat gaacgacatc actggcggac gtactccggc
    13741 ctccttcgag ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa
    13801 attcgctggt gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc
    13861 gattggtcgc acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtcggtgc
    13921 gactggattc gacccgaaag tgagcctcga tgacccggaa gcgttaacca aaatccgtcg
    13981 cgaactgaaa gatgctggcg cagagcgtat ctggtacatc gccgatgcct tccgtgcggg
    14041 cctgtctgtg gacggcgtgt tcaacctgac caatattgac cgctggttcc tggtacagat
    14101 tgaagaactg gtgcgtctgg aagagaaagt agcggaagtg ggcatcactg gcctgaacgc
    14161 tgaattcctg cgccagctga aacgcaaagg ctttgccgat gcgcgtctgg caaaactggc
    14221 gggcgtgcgc gaagcggaaa tccgcaagct gcgcgaccag tatgacctgc acccggttta
    14281 taagcgcgtg gatacctgtg cggcagagtt cgccaccgac accgcttaca tgtactccac
    14341 ttatgaagaa gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggttctcgg
    14401 cggcggcccg aaccgtatcg gtcagggtat cgaattcgac tactgctgtg tacacgcctc
    14461 gctggcgctg cgcgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt
    14521 ctccaccgac tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt
    14581 gctggaaatc gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac
    14641 cccgctgaaa ctggcgcgcg cgctggaagc tgctggcgta ccggttatcg gcaccagccc
    14701 ggatgctatc gaccgtgcag aagaccgtga acgcttccag catgcggttg agcgtctgaa
    14761 actgaaacaa ccggcgaacg ccaccgttac cgctattgaa atggcggtag agaaggcgaa
    14821 agagattggc tacccactgg tggtacgtcc gtcttacgtt ctcggcggtc gggcgatgga
    14881 aatcgtctat gacgaagctg acctgcgtcg ctacttccag acggcggtca gcgtgtctaa
    14941 cgatgcgcca gtgttgctgg atcacttcct tgatgacgcg gtagaagttg acgtggatgc
    15001 catctgcgac ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg
    15061 cgtgcactcc ggtgactccg catgttctct gccagcctac accttaagtc aggaaattca
    15121 ggatgtgatg cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat
    15181 gaacgtgcag tttgcggtga aaaacaacga agtctacctg attgaagtta acccgcgtgc
    15241 ggcgcgtacc gttccgttcg tctccaaagc caccggcgta ccgctggcaa aagtggcggc
    15301 gcgtgtgatg gctggcaaat cgctggctga gcagggcgta accaaagaag ttatcccgcc
    15361 gtactactcg gtgaaagaag tggtgctgcc gttcaataaa ttcccgggtg ttgacccgct
    15421 gttagggcca gaaatgcgct ccaccgggga agtgatgggc gtgggccgca ccttcgctga
    15481 agcgtttgcc aaagcgcagc tgggcagcaa ctccaccatg aagaaacacg gtcgtgcgtt
    15541 gctttccgtg cgcgaaggcg ataaagaacg cgtggtggac ctggcggcaa aactgctgaa
    15601 acagggcttc gagctggatg cgacccacgg cacggcgatt gtgctgggcg aagcgggcat
    15661 taatccgcgt ctggtgaaca aggtgcatga aggccgtccg cacattcagg accgcatcaa
    15721 gaatggcgaa tatacctaca tcatcaacac cacttcaggc cgtcgcgcga ttgaagactc
    15781 ccgcgtgatc cgtcgcagtg cgctgcaata taaagtgcat tatgacacca ccctgaacgg
    15841 tggtttcgct accgcgatgg cgctgaatgc cgacgcgacc gaaaaagtaa tttcggtgca
    15901 ggaaatgcac gcgcagatca aataatagcg tgtcatggcg gatatttttc atccgctaat
    15961 ttgatcgaat aagtaatacg gttctctgat gaggaccgtt ttttttgccc attaattaaa
    16021 tcttttgggg aatcgatatt tttgatgaca taagcaggat ttagctcaca ct
//
LOCUS       DAJTVK010000092        15961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000092 DAJTVK010000000
VERSION     DAJTVK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.092"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO372_002972"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="OO372_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6342407.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO372_002973"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="OO372_002973"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6342408.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO372_002974"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="OO372_002974"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR6342409.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO372_002975"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="OO372_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO372_002976"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="OO372_002976"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6342410.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO372_002977"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="OO372_002977"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR6342411.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="OO372_002978"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="OO372_002978"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6342412.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="OO372_002979"
     CDS             8229..8939
                     /locus_tag="OO372_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6342413.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTPLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="OO372_002980"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="OO372_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6342414.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="OO372_002981"
     CDS             9636..10343
                     /locus_tag="OO372_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6342415.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="OO372_002982"
     CDS             10343..10891
                     /locus_tag="OO372_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6342416.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="OO372_002983"
     CDS             10901..12067
                     /locus_tag="OO372_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6342417.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="OO372_002984"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="OO372_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="OO372_002985"
     CDS             13576..14229
                     /locus_tag="OO372_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6342418.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="OO372_002986"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="OO372_002986"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR6342419.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="OO372_002987"
     CDS             complement(15612..>15961)
                     /locus_tag="OO372_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6342420.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3699 a         4346 c         4162 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgcccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAJTVK010000093        15926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000093 DAJTVK010000000
VERSION     DAJTVK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.093"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..168
                     /locus_tag="OO372_002988"
     CDS             <1..168
                     /locus_tag="OO372_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6342421.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="OO372_002989"
     CDS             complement(340..669)
                     /locus_tag="OO372_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6342422.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="OO372_002990"
     CDS             complement(674..859)
                     /locus_tag="OO372_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6342423.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="OO372_002991"
     CDS             complement(856..3495)
                     /locus_tag="OO372_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6342424.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="OO372_002992"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="OO372_002992"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6342425.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="OO372_002993"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="OO372_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR6342426.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="OO372_002994"
     CDS             complement(5222..5488)
                     /locus_tag="OO372_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6342427.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="OO372_002995"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="OO372_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6342428.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9652..10785
                     /gene="ompN"
                     /locus_tag="OO372_002996"
     CDS             9652..10785
                     /gene="ompN"
                     /locus_tag="OO372_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR6342429.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10926..11360
                     /gene="uspF"
                     /locus_tag="OO372_002997"
     CDS             10926..11360
                     /gene="uspF"
                     /locus_tag="OO372_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6342430.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11946..12860
                     /gene="sitA"
                     /locus_tag="OO372_002998"
     CDS             11946..12860
                     /gene="sitA"
                     /locus_tag="OO372_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6342431.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12860..13687
                     /gene="sitB"
                     /locus_tag="OO372_002999"
     CDS             12860..13687
                     /gene="sitB"
                     /locus_tag="OO372_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6342432.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13684..14541
                     /gene="sitC"
                     /locus_tag="OO372_003000"
     CDS             13684..14541
                     /gene="sitC"
                     /locus_tag="OO372_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6342433.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14538..15395
                     /gene="sitD"
                     /locus_tag="OO372_003001"
     CDS             14538..15395
                     /gene="sitD"
                     /locus_tag="OO372_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6342434.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15596..>15926)
                     /locus_tag="OO372_003002"
     CDS             complement(15596..>15926)
                     /locus_tag="OO372_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6342435.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3984 a         4022 c         3830 g         4090 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcata
     9421 taacccgcgc agaataacgt atagaaaatt aaaagtataa tttttatttt gtatgtatta
     9481 tcttcgatac attcaccaga cttattatca tttcagaaga attatttgat taaggtttta
     9541 cttaaggcgt aacaaatgat atttatcggc taactgaact tctcctttag gatgttttca
     9601 ctccccggaa tggggataaa aggcaaataa aataactaaa ggatttattc aatgaaaagc
     9661 aaagtactgg cacttttaat tcctgccctg ctcgccgcag gtgctgcaca tgcagccgaa
     9721 gtttataata aagacggcaa caaattagat ctgtatggca aagttgatgg cctgcattat
     9781 ttttctgata attcagcgaa agatggcgat cagagctatg cgcgtctggg ttttaaaggc
     9841 gaaacccaaa ttaacgatca actcactggc tacggtcaat gggaatacaa tattcaggca
     9901 aacaacactg aatcttcaaa aaaccagtca tggacccgtc tggcatttgc cgggctgaaa
     9961 tttgcagatt acggttcttt cgattacgga cgtaattatg gcgtaatgta cgacatcgaa
    10021 ggctggaccg atatgctgcc tgaatttggc ggtgactctt ataccaatgc agacaacttt
    10081 atgactggtc gtgccaacgg cgtcgcgact tatcgtaata ctgatttctt cggtctggta
    10141 aatggtctga acttcgcggt gcagtatcaa ggtaacaacg aaggtgccag taatggtcag
    10201 gaaggcacca acaacggacg tgatgttcgc catgaaaatg gtgacggctg gggtctttcc
    10261 acaacatatg atttaggcat gggctttagc gctggtgcgg catacacctc atctgaccgc
    10321 accaatgacc aggttaacca tactgcggcg ggtggtgata aagcagacgc gtggactgct
    10381 gggctaaaat acgatgctaa caatatttac ctggcaacca tgtattcaga aacgcgtaat
    10441 atgaccccgt ttggcgacag cgattatgct gttgcaaaca aaacccagaa ctttgaagtc
    10501 actgcacagt accagtttga ttttggtctg cgtccggcag tctctttcct gatgtctaaa
    10561 ggccgtgacc tgcacgctgc cgatggtgca gacaacccgg caggtgttga tgataaagat
    10621 cttgttaaat acgccgatgt tggcgcgact tactatttca ataaaaacat gtccacttat
    10681 gttgactata aaatcaacct gttggatgaa gatgacagtt tctacgctgc caacggcatc
    10741 tctaccgatg atattgtcgc tttaggtctg gtttatcagt tctaaatcct cctgcccgct
    10801 gttatggcgg gctttttctg cttattcttc ctcctttgat ctaaattaaa aatgtgaact
    10861 ccgtcattac acaaaaagtg tcatctggcg ttacacttta tgcggatact aaaacaggag
    10921 gttttatgaa cagaacgatt cttgtcccta tcgatatttc cgattcagaa ttaactcaac
    10981 gcgtgattag ccacgttgag gcagaggcaa agattgacga tgcagaggtt catttcctga
    11041 cggtaatacc ttcactgcct tactatgcct ctctgggttt agcgtattcc gcagaattac
    11101 cggcaatgga tgacctgaaa gcggaagcca aatcgcaact ggaagagatc attaaaaaat
    11161 ttaaactgcc aaccgacaga gtgcatgtcc atgttgagga aggctcgccc aaagaccgca
    11221 ttctggaatt ggcgaagaag atccccgctc atatgatcat cattgcttcc catcgaccgg
    11281 atatcaccac ttatctgctc ggttccaacg ccgcagctgt agtgcgtcac gcagagtgct
    11341 ccgtgctggt tgtgcgctga cactaacgcc cgcacattgc tgcgggcttt ttgattcatt
    11401 tcgcaaatgt gctgacattt tcccctctaa tccgtaccat acacgccaca gttttatatc
    11461 agatttctta tgctgggcgt tccggcatga cgcatactct tctgatgcca tataacgaat
    11521 tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga
    11581 acaagtaagg gcaaaaatca caactatctg acccataagt attttattta tttttcaata
    11641 tgttaaacat tatcagcgac aacaataagc tacgataacc cactcttttt ttgccccttt
    11701 tttgcccctt ctacagcgtt ttgccccatt tttgccatca acaattttcc tcataaagtc
    11761 atttcaaagc ctaatagctg ctctactttt ttcctcaata tcatgtcgcc agcaggaaac
    11821 aacctaatcc ctgttttagg cctcaaagct gttaatgata atcattttca ttttgtatgg
    11881 gttatgctat aaaacatagc cgttgctatg tttgttctaa ttttgctcac tataggtact
    11941 aaattatgca ctcaataaaa aaagtaacca tgctcttggg gtgcctcgcc ctcacctgct
    12001 cgatcgcatt tcaggcaagt gcaactgaaa aattcaaggt cattacaaca tttaccatca
    12061 tcgcggatat ggccaaaaac gtggctggag atgctgcaga agtctcatcc ataaccaagc
    12121 ctggtgcaga aattcatgag tatcagccta cccctggcga tattaaacgt gcgcaggggg
    12181 cacaactgat tctcgccaat ggtatgaatc tggaattgtg gttccaacgc ttttaccagc
    12241 atctcaatgg ggttccagaa gtaattgtct cttcgggggt gacgccagta gggatcaccg
    12301 aaggacccta tgagggcaaa cctaatcccc atgcatggat gtcgccagat aatgctctga
    12361 tttacgtcga taatattcgt gatgcgttga taaaatacga tccggcaaat gcacaaacct
    12421 accaacgtaa tgccgatact tataaagcca agattaccca aacccttgcc cccctgcgta
    12481 agcagattac ggaactccct gagaatcagc gatggatggt caccagtgaa ggggcttttt
    12541 cttatctcgc acgcgatttg gggctaaaag agctttatct gtggccgatt aatgccgatc
    12601 aacaaggaac accgcagcag gtacgtaagg ttgttgatat agttaagaaa aatcatatcc
    12661 cggcagtctt tagcgagagt acgatttccg ataaaccagc gcgtcaggtt gcgcgtgaaa
    12721 ccggcgcaca ctacggcggt gtgctctatg tcgattccct gagcacagaa aacggcccgg
    12781 tacctacgta tatcgacctt cttaaggtta ctaccagtac gctggtacag ggaattaaag
    12841 ccgggaaaag ggagaaataa tgatgcaatc tgcaggcatt gtcgttaatg atgttactgt
    12901 cacctggcgt aacgggcaca cagcactgcg cgatgcatcc ttcacagtac caagtggatc
    12961 tattgccgct ctggttgggg taaacggttc cggaaaatcg acgttgttta aagcgattat
    13021 gggatttgtg cgtctgacca gcggaaagat atctgttctg ggtattccca cgcgacaggc
    13081 gctacagaaa aacctggttg cctacgtccc tcagtcagaa gaggttgact ggtcatttcc
    13141 tgtactggtg gaagatgtgg tgatgatggg ccgctatggg catatgggta tgttgcgtat
    13201 agccaaaaag agggatcgtc agattgtcac cgatgcgctg gaacgtgtcg atatggtgga
    13261 ttttcgccat cgtcaaatcg gcgagctttc cggtggacaa aagaaacgcg tctttctggc
    13321 gagagcgatt gcacagcagg gtgatgtgat cctgctggat gaacctttta ccggcgttga
    13381 tgtgaaaacc gaagcgaaaa tcatcagcct gttgagggag ttacgcgcgg aaggcaaaac
    13441 gatgcttgtc tcaacgcaca atctcggctc agtcacgaca ttttgcgatt atacggtcat
    13501 ggtcaaaggc accgtcctgg caagcgggcc gacagacacc acttttacag ccgagaacct
    13561 ggagctggct tttagcggcg tactgcgcca tgtcacactt aacgactcgg aagaaagcat
    13621 tattactgac gatgaacgcc cttttgtggc acatcgaccg tcagcggtgc agagggaagc
    13681 aagatgaacg tgctactgga acccttcagc tatgaatata tgctcaatgc gatgtgggtc
    13741 tcggcaatgg tcggcggtct ctgcgctttc ctgtcatgct atctgatgct aaaaggctgg
    13801 tcgctgattg gcgatgcgct gtcgcactct attgtcccgg gtgttgcggg ggcatatatg
    13861 ctggggcttc cgttttctct gggagccttc ttttccggcg gactggcagc gggcagcatg
    13921 ctgtttctta accaacgcac ccgcttaaaa gaagatgcca tcatcggcct gatcttctcg
    13981 tccttttttg gcctggggct gtttatggtg tcattgaacc cgacatccgt gaacattcag
    14041 accatcgtcc tgggcaatat tctggcaatc gatccggcgg atatcctgca actcactatc
    14101 attggcattc tctcgatcat cgtgcttttt ttcaaatgga aagatcttat ggtgaccttt
    14161 ttcgacgaga atcacgcccg tgctattgga ttacatccgg gcagattaaa aatcctcttc
    14221 ttcaccctgc tgtcggtttc gaccgttgcg gcgctgcaaa cggttggtgc ctttctggtg
    14281 atctgtctgg tggtgactcc cggtgcaaca gcatggttgt taaccgatcg ttttccccgc
    14341 ctgctcataa ttgctgtaac tattggcagt gtaaccagtt tcctcggcgc gtgggtcagt
    14401 tactttctgg acggcgcgac aggtggaatc atcgtggtgg cgcagacact gctgtttctt
    14461 ttagcatttg tatttgcgcc cacccatgga cttttggcaa atcgtcggcg tgcgcataaa
    14521 gcgctggagg atcgctcatg atggcattat tactggagcc actgcagttc acctttatga
    14581 gtcacgcact gctgatttca ctggtagttt ccataccctg tgcgctgctg tcggtctttc
    14641 tggtcctgaa aggttgggca ctgatgggtg acgcgatgag tcatgcggtt tttcctggca
    14701 tcgtgcttac ctggattctg gggttacctc tggctacagg ggcattcgtc gccggagtat
    14761 tctgcgcggt ggcgacggga tacctgaaag acaacagccg aatcaagcaa gatacagtta
    14821 tggggattgt tttttctggc atgtttgccg caggtctgat cttgtatatt gcaatcaaac
    14881 cagacgtaca tcttgaccat attcttttcg gcgacatgct cgggataacc atcggcgata
    14941 taatccagac ggtgattatt gccgggctgg ttacacttgt tattagcgca aaatggcggg
    15001 attttttgct gttcagcttt gattatcaac aggcgcaggt aagcggtttg catacgagat
    15061 ggctgcatta tgggctgctg tgcatggttt ccctgaccat tgtggcgacg ctgaaagcag
    15121 taggtatcat tctctctatt tctctgctca ttgcgcctgg cgccattgca gtactcctga
    15181 cacaacgctt tcatattgcg ctcctactgg cgactggcat atcggtaata gtgtcaatga
    15241 ctggtgtctg gctgtccttt tttatcgaca gcgcaccagc tccgacgatt gtcgtcttat
    15301 tcgcggtcgt gttcatcatg acgtttaccg ttaccagcat caacgcacgc acaaagggaa
    15361 acgcccatac acaggatctg ttatcaccca attagcacaa catccgagga cagcatcgtc
    15421 cgccagccta cagaagtccg gaaagccatg gaaggtcgtt gggaggaggt acgaattcta
    15481 tcatgcaaaa aatacgtaaa atcgataacg cctagaaatc attcaatact cgcactatcg
    15541 aaagtttacc agccaaccgc ggcacgttcc tgcatacgac gtgccgcagc ttctgttatt
    15601 ccacgataaa attcctaacc gaattcaaat ccttcagact attcagctct tccttcagct
    15661 cccgctgacg gcgataaatc tcgtcattgc gctcaacctg tgcctgagcc attgctgccg
    15721 ccagttcttc cagttccggc atcgactgtt tcacctgctg attatcggca tcaccccacg
    15781 tcatcacgtc ccgtacaatg tcggatttcg cagccattac caccggataa aggcggccca
    15841 gtgagttggg gccagcattc cagatacgac cgttccattc aaacgtgaac ggcttcgcct
    15901 cctgttctgt gcgccatgct tcaatt
//
LOCUS       DAJTVK010000094        15883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000094 DAJTVK010000000
VERSION     DAJTVK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.094"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1455
                     /locus_tag="OO372_003003"
                     /pseudo
     CDS             1..1455
                     /locus_tag="OO372_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1543..2514)
                     /gene="lpxM"
                     /locus_tag="OO372_003004"
                     /gene_synonym="msbB"
     CDS             complement(1543..2514)
                     /gene="lpxM"
                     /locus_tag="OO372_003004"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6342436.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2634..3956)
                     /gene="mepM"
                     /locus_tag="OO372_003005"
     CDS             complement(2634..3956)
                     /gene="mepM"
                     /locus_tag="OO372_003005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6342437.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3972..4904)
                     /gene="znuA"
                     /locus_tag="OO372_003006"
     CDS             complement(3972..4904)
                     /gene="znuA"
                     /locus_tag="OO372_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6342438.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4983..5738
                     /gene="znuC"
                     /locus_tag="OO372_003007"
     CDS             4983..5738
                     /gene="znuC"
                     /locus_tag="OO372_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6342439.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5735..6520
                     /gene="znuB"
                     /locus_tag="OO372_003008"
     CDS             5735..6520
                     /gene="znuB"
                     /locus_tag="OO372_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6342440.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6667..7677)
                     /gene="ruvB"
                     /locus_tag="OO372_003009"
     CDS             complement(6667..7677)
                     /gene="ruvB"
                     /locus_tag="OO372_003009"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6342441.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7686..8297)
                     /gene="ruvA"
                     /locus_tag="OO372_003010"
     CDS             complement(7686..8297)
                     /gene="ruvA"
                     /locus_tag="OO372_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6342442.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8436..8501)
                     /gene="yobI"
                     /locus_tag="OO372_003011"
     CDS             complement(8436..8501)
                     /gene="yobI"
                     /locus_tag="OO372_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6342443.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8574..9176
                     /locus_tag="OO372_003012"
     CDS             8574..9176
                     /locus_tag="OO372_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6342444.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9178..9699)
                     /gene="ruvC"
                     /locus_tag="OO372_003013"
     CDS             complement(9178..9699)
                     /gene="ruvC"
                     /locus_tag="OO372_003013"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6342445.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9734..10474)
                     /locus_tag="OO372_003014"
     CDS             complement(9734..10474)
                     /locus_tag="OO372_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6342446.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10503..10955)
                     /gene="nudB"
                     /locus_tag="OO372_003015"
     CDS             complement(10503..10955)
                     /gene="nudB"
                     /locus_tag="OO372_003015"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6342447.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11073..12845)
                     /gene="aspS"
                     /locus_tag="OO372_003016"
     CDS             complement(11073..12845)
                     /gene="aspS"
                     /locus_tag="OO372_003016"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6342448.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13155..13721
                     /locus_tag="OO372_003017"
     CDS             13155..13721
                     /locus_tag="OO372_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6342449.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            complement(13803..13979)
                     /locus_tag="OO372_003018"
     CDS             complement(13803..13979)
                     /locus_tag="OO372_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342450.1"
                     /translation="MGQKYLLWGILGHDWDTLLHMNFADFHMAWQSNALIILKKTHAL
                     GRSLVIFKNPVSRS"
     gene            14076..14324
                     /locus_tag="OO372_003019"
     CDS             14076..14324
                     /locus_tag="OO372_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6342451.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            14925..>15883
                     /locus_tag="OO372_003020"
     CDS             14925..>15883
                     /locus_tag="OO372_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6342452.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPEIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
BASE COUNT         4039 a         4029 c         3642 g         4173 t
ORIGIN      
        1 gaatttttta taactattac taataatatg acatcaattc ttcgcagtga gtttgatgaa
       61 atatggaaag cgatgctgac cttgcaattc catgacatcc ttcccggctc atgtatttca
      121 agagtttatc atgaaactga aaaggaatat cttaaactag aagcaaaaac cgagaaaatt
      181 atttctgatg cgcagtctac tttattaagt aaaattgaca cttcttctta taaagatccg
      241 cacattttgt ttaataccac ctgttttgct cgtaacgaat ggatcaatat aaacaacaac
      301 tggctcaaag cacgagtaaa ctcttatggt tatgctgtga ttgttccaaa gaataagatc
      361 gtaaatggtc tgaaggcaga atctcaaagt attgaaaata attacattaa acttcttttt
      421 tcagaaagcg gtgatttaat ttcgctatat gataaaagat atggtaaaga atacattact
      481 gaaaatatgc acagtgagat ccgtgcatac cacgaagatg caggtttttt cgcggcctgg
      541 gattttgctt caaattatcg tgatggggaa tcatatgttt tactcgctga aaaaatgaca
      601 actgtgatca gcgggcctaa aactacgatg acgctgattt atcactacaa ttcctcttat
      661 cttcgtttag cctttaccct gacacaggat agtccacgcg ttgatgtcca aacgtttatt
      721 gattggcacg aacctaatgt ttctctcaaa gtaaaattcc cggtgtcagt acaaacatct
      781 cttgcacaat gtcaaattca gttcggtgta attgacagac caacgcatag cgatgattcg
      841 ttcgcttttg ccaaagatga aataccagca catcactggg tcgacctttc tgatcgagaa
      901 actggcatag ctttaatagc aaaaaacaaa tatggctatc gtgtcaaaga ccagacgctt
      961 gaattaactt tgctgcgttc gcagcataaa cctggggaag ttgttaagac agacaattat
     1021 gataatttcc tgattaataa ttttgcagat attggtaaac aaaaattcat attctcctta
     1081 tttcctcatc atggagatta ccgtgtgggt ggtgttatta atcaagccta tataatgaat
     1141 catcccttac aggtcgtccc tgtcaaaccg cacccgggtg aattaccccc ttcactatca
     1201 ttttttgcta tcgatactaa cagcgttatc atcgaaacaa taaaattagc tgaagacggc
     1261 gacggtataa tcgtaagatt atatgagagt catggattag aaatttcagc catgctttcg
     1321 tgagtatgta attatcacta cgttcaatat gtcgatcttc aagaaaataa gttagatgac
     1381 agtttttatt gcaatcaata ttgcaatctt gagttcaaac cttttgaaat tatcacgcta
     1441 cgattaactc aatagcgtac taacctaaaa actcaaaata gtgggaacac tcattgagat
     1501 tttttatcga gtacagcctc tcaataattt gagaggcttt ttttatttga taggataaag
     1561 atctttacgc ttatacggtt ggatttcgcc cggtttgcga gttttcagca attttaatat
     1621 ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg acttcttcat tcatccgccg
     1681 cgcaatcgta tgatcatccg cctctaacag atcatccatc ggtgggcgca cctgaatcgt
     1741 cagacggtgc gtcttgccat cataaatcgg aaacagcgga acaacgcgcg cacggcacac
     1801 tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag gtggcaaaga aatccacaaa
     1861 ttcgctgtgc tctgggccat gatcctgatc gggtaaataa tatccccagt acccctgacg
     1921 taccgactgg atgaatggtt taataccgtc atttctcgca tgcagacgac cgccaaagcg
     1981 acgacgcacc gtgttccaga cataatcaaa aaccgggttg ccctgattat ggaacatcgc
     2041 tgccattttc tgcccttgcg aggccatcag catggcagga atatcgacgg cccaaccgtg
     2101 cggcaccaga aagataactt tctcgttatt acgcctcatc tcttcgatga tctccagccc
     2161 ttgccagtca acgcgcggct gaattttctc cggtccgcgt attgccaact cagccatcat
     2221 tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca atcgcttcgc gttcagcttc
     2281 actacgttct ggaaagcaga gcgacagatt gattaacgca cgacggcgtg agctttttcc
     2341 cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga tcacggaact ttggcggcgt
     2401 taaagcgata cccgccatcg ctgctacacc cagccatgct ccccagtagc gcgggtggcg
     2461 aaaggattta tcaaactcag gaatgtattc gctattattt tttttcgttt ccatgctttt
     2521 ccagtttcgg ataaggcaaa aatcaatctg gtgatagtat agcggcgcaa cttgccccgc
     2581 accaaataaa aaaagccgat actgactgcg taccggctgc gaatggatgt taattaatca
     2641 aaccgtagct gcggcacaat ctctttggcc tgcgccagga attcgcgacg atcggagccg
     2701 gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg ctggtttatc
     2761 catacttcat agtgcagatg cggcccggtt gaacgtccgg tattaccgga aagcgcgata
     2821 cggtcgccac gtttcacctt ctgtcccggt ttcaccagaa tcttgcgcaa gtgcatataa
     2881 cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc gccactgcgt
     2941 ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccctg cggcatggcg
     3001 aaatcaacac ctctgtgtgc tgcaacgcga ccggtcaccg gattagtacg acgcgggtta
     3061 aagttagacg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga
     3121 ccagtaccgt tacgatcgta gaatttgcca tcttcagcgc ggatagcgta ataatcttta
     3181 ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc atcaagcatt
     3241 tctcgagaca ttaacaccgc aaattcatcg ccttttttca gtttgcggaa atccatttgc
     3301 cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg
     3361 ctggcaacaa agctcccccc gacggtacct ttcagcagat tgttaaccca ctctccttgc
     3421 tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt tcgggtttca
     3481 cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag
     3541 agttgttgac cgattttcag gttacgcaat tctttgtcgg ccgcagccag ttgggtgata
     3601 tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc agtggaaaca
     3661 acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc ctggggaatg
     3721 gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga
     3781 atttcgttct gttccagctc aatggttttg acaattggcg tggcatcgcg gtgataaaca
     3841 tagggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa cataacgcgg
     3901 tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg ttgcacgtaa
     3961 tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa ttgactcagg
     4021 aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc caacgttccc
     4081 atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca
     4141 gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg taaacgctgc
     4201 gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag tccgaactgt
     4261 ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg
     4321 agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc cttcaggttg
     4381 gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg gattgcaacc
     4441 gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc gccgtgatgg
     4501 tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat catcgccgtg aatacttttc
     4561 atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta cctgcttcgc tcctggtaat
     4621 ttgcttaccg gtttttgcat aaacgcttcc atctccgggc caacccaaac gactaagtcc
     4681 gcgttctgta agcgttttac atccgatggg cgcagtgaat aatcatgttc tgaagcgccg
     4741 tcaggaagta aaacctctgt ttctgttacc ccatcagcaa tggcagaagc gatgaaccca
     4801 acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc tccccagaga
     4861 gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg accaatcatc
     4921 gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag actaaaatta
     4981 acatgacaag tctggtttcc ttggaaaatg tctcggtttc ttttggccaa cgccgcgtcc
     5041 tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt gggccaaatg
     5101 gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct ggtaacaccc gatgaagggg
     5161 ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc gcagaagctg tatctcgaca
     5221 ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggtaca cataaagaag
     5281 atattttgcc tgcactgaaa cgtgtccagg ccgggcacct gattaacgca ccgatgcaaa
     5341 agctctctgg tggcgaaacg cagcgtgtac tgttagcgcg agcattgtta aatcgcccgc
     5401 aattattagt gctggatgaa cccactcaag gcgtggatgt aaatggccag gtggcgttat
     5461 atgaccttat tgaccaactg cgtcgtgaac tggattgtgg cgttttaatg gtttctcacg
     5521 atctgcatct ggtgatggca aaaaccgatg aagtgctgtg cctgaatcac cacatttgtt
     5581 gttccggcac accggaagtt gtttccctgc atccggagtt tatttcaatg tttggtcctc
     5641 gtggtgctga acaactgggt atctatcgcc atcatcataa ccatcgtcac gatttacagg
     5701 gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat ttcccggttg
     5761 gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg
     5821 tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggtg tcgcgtttgg
     5881 tttgttgctg gacgtgaatc cattctatgc ggtgattgcc gttacgctgc tgctggcggg
     5941 cggtctggta tggctggaga agcgtccaca gctggcgatc gacacgttat tagggattat
     6001 ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta atattcgtgt
     6061 tgatttgatg gcttacctgt tcggtgattt gctggcagtg acgccagaag atctcatctc
     6121 tattgcgatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc gcaatttgct
     6181 gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa
     6241 attgttgttg atgctggtga cggcattgac gattggtgta gcgatgaaat ttgtcggtgc
     6301 gttgattatt acttcgttgc tgattattcc tgcagctact gcgcgtcgct ttgcccgtac
     6361 gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtaa ctggcggttt
     6421 aaccttttcc gcgttttacg atacgccggc gggtccgtcg gtagtgctat gtgctgcact
     6481 gttatttatt atcagtatga tgaaaaagca ggccagctaa tctgtcgctg aacacatttg
     6541 tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggt aggccggata
     6601 agacgcgaat agcgtcgcat caggcacact gccaatgtcg gatgcggtgc gagcgaccaa
     6661 tccgacttac ggcatttctg gcggcgttat gccaaagtga ttccacgccc gcgtcgtcgc
     6721 catacgccca cgcggtgtac gctgcaaaaa gccttgctga atcagataag gctccagcac
     6781 atcctcaatg gtttcacgtt cttcgccaat ggctgccgcc aggttatcca gacctaccgg
     6841 cccaccaaag aacttatcga ttaccgccag caacaatttg cggtccatat aatcgaaacc
     6901 ttcagcatcg acattcaaca tatccagcgc ctgagcagcg atatctgccg agatggtgcc
     6961 atcgtgcttc acttcggcga aatcacgcac tcgacgcagc agacggttgg caatacgtgg
     7021 cgtaccgcga gcgcgacgag caacttccag cgcgccgtca tcactcatct caagccccat
     7081 aaagcgtgcg ctgcgactga cgatatattg cagatccggc acctgataaa actccagacg
     7141 ttgcacaata ccaaaacggt cgcgcaacgg tgatgtcagc gaacctgcgc gcgtggttgc
     7201 accaatcagg gtaaacggcg gcaaatcaat tttaatggag cgtgccgccg gaccttcacc
     7261 aatcatgata tccagttggt agtcttccat tgccgggtac agcacttctt caacaactgg
     7321 cgatagacgg tggatctcat caataaacag cacgtcatgc ggttcaaggt tagtgagcat
     7381 cgctgccaga tcgcccgcct tttccagcac cggaccagaa gtcgtgcgta aattaacgcc
     7441 catttcattg gcgacaatgt tggcaagcgt agttttaccc aaccccggag gaccaaaaat
     7501 caacagatga tcgagggcat cgccgcgcag tttcgctgct ttgatgaaaa tctccatctg
     7561 tgaacgaacc tgcggctgac caacatactc ttccagtaat ttagggcgaa tggcgcgatc
     7621 tgccccatct tccggcaaag tggtaccggc agaaatcaga cggtctgctt caatcatcct
     7681 ttacctcata acgcggcgcg tagggcttcg cgaattaatg tttcactgct ggcgtcagtg
     7741 cgagcgattt tgctcaccat gcggcttgct tcttgtggtt tatagcccag cgccaccagc
     7801 gcggcaaccg cttcctgttc agcatcgtcg gttgccgggc tggcaggcga cgtgagcacc
     7861 agatcggcgg ctggcgtaaa gagatcgccg tgcaaacctt taaatcggtc tttcatttcg
     7921 acaattaaac gctcggcggt ttttttacca atgcccggca gtttcaccag tgctcccact
     7981 tcttcacgtt caacggcatt aacgaactgc tgcgctgaca ttccggagag gatcgccagc
     8041 gccaacttcg ggccgacgcc gttggttttg attaactctt taaacaacgt gcgctcttgt
     8101 ttattgttaa aaccgtacag cagttgcgcg tcttcacgca ccacaaagtg ggtgaaaacg
     8161 atcgcttcct gacccgcttc agggagttca taaaaacagg tcatcggcat atgcacttca
     8221 tagcctacgc cgcccacttc aattaacacc agcgggggtt gtttttcaat gatgatgcct
     8281 ctgagtctgc ctatcacatg acgctcctgc gtaatgaatc aaagataatg ctgtatgata
     8341 aaaaaatgct ggatagatat ccagcgaagg atgaagaaaa cttgcgaggt gtctcgatga
     8401 actgaaaaat ggcacagtat aatttattct acaggttata ttggaagcaa atattttaat
     8461 attacatatt cagtgaataa atgcgtaata aaaatataca ttgcgcctcc tgaaaaaaaa
     8521 taaatttttt atgctattac gtatattcgt atctatttca atggaatgac aacgtgaata
     8581 ttaattaccc tgctgaatat gaaattggag atatcgtctt tacatgtata ggtgctgcct
     8641 tatttggtca aatatcagct gcatcaaatt gctggagtaa tcacgtcggg atcattatcg
     8701 gtcataacgg tgaagacttc ctggttgcag aaagccgggt tcccctttca accatcacca
     8761 cgctatctcg ttttattaaa cgttcgtcta atcaacgcta tgctataaag cgattagacg
     8821 ctgggctgac agaacaacaa aaacaacgaa ttgttgaaca ggttccctcc cggctacgca
     8881 aactttacca caccggtttt aaatacgaat cttcgcgcca gttctgttca aaatttgttt
     8941 ttgatattta taaagaagcg ctatgcattc cggtgggtga aatagaaacg tttggagaac
     9001 tgttaaatag caatccgaat gcaaaactca ctttctggaa attctggttc ttaggttcta
     9061 ttccgtggga gcgtaaaacc gtcacgccag ccagtttgtg gcatcatccg ggtctggtgt
     9121 tgattcacgc ggagggagtt gaaacgcctc agcctgaact gaccgaggcg gtataactta
     9181 acgcagtcgc cctctcgcca ggttcagccg cgattcgctc atctgcattg cattctggct
     9241 gacgtgacaa tgggtaatcg cgatagctaa cgcatcggcg gcatccgcct gtggattagc
     9301 gggcagtttc agcaaggtgc ggaccatatg ctgcacctgg cttttttcgg cactaccaat
     9361 acctaccacc gtttgcttta cctgacgtgc cgcgtattca aataccggca attcctgatt
     9421 caccgccgcc acaatcgcca cgccgcgcgc ctgccccagt ttcagagccg agtcggcatt
     9481 tttggccata aagacttgtt caatggcgaa ataatctggc tggaactggg tgatgatttc
     9541 cgtcacgccc gcatagatga gcttcagacg agacggtaaa tcatccactt tggtgcgtat
     9601 gcatccgcta cccaggtagg acagttgcct gcctacctgg cggatgacac catagccgat
     9661 cacgcgcgaa cccgggtcaa tgccgagaat aatagccatc acgcgtctcc gttttgctgt
     9721 ttagcaggcc ccatcagaga gttgctgcaa cctcatcaga gatttcaccg ttatggtaga
     9781 cttcctgcac gtcgtcgcaa tcttccagca tatcgatcag acgcatcagt ttcggtgcgg
     9841 tttctgcatc catatcagct ttggtggacg ggatcatgga aacttccgcg ctgtctgctt
     9901 tcagacctgc cgcttccaga gcgtcgcgca ctttacccat ttcttcccat gcagtgtaga
     9961 cgtcaatcgc gccgtcatca taggtcacaa cgtcttcagc acctgcttcc agtgctgctt
    10021 ccataatggt gtcttcatcg cctttctcga aggagatcac gccttttttg ctgaacagat
    10081 aggcaacgga accatcagta ccgaggttac cgccacattt gctaaatgca tgacgcactt
    10141 cagcaacggt acggttgcgg ttgtcagaca gacattcaat catgattgcc gtgccgccag
    10201 gaccgtaacc ttcgtagatg atggtttcca tgtttgcatc atcatcaccg cccacaccac
    10261 gtgcaattgc gcggttcagt gtgtcacggg tcatgttgtt agacagtgct ttatccaccg
    10321 ccgcacgcag acgcgggtta gcgtccggat cgccaccgcc cagcttagcc gcggttacca
    10381 gctcacgaat gattttagtg aagattttac cgcgcttagc atcctgcgca gctttacgat
    10441 gtctggtgtt ggcccattta ctatgacctg ccataaaaat atctccagat agccctgcct
    10501 gttcaggcag cgttaattac aaactgttca atcgcctgcc ggttgctcca ggacttagtg
    10561 agcgccgcgg cagcaggcgc atcaagccac ttgtaagcca gatgttcagt gaagactacc
    10621 tggcgttcgt gcgggagcgc aagacagaac catgattccg tattacgcgt cacgcccggc
    10681 gcatagcgat gacgtaaatg tgaaaaaatt tcaaactcta ccgtgcgctg acagtcaatt
    10741 aaggtcagtt gttcagcgac aacatcaatg gtgacctctt cctttacttc gcgcatggca
    10801 gcttgcggcg cggtttcacc ctcttccacg ctgccggtta ccgactgcca gaaatcggga
    10861 tcgtcacgcc gctgcaacat cagcacccgt ttcgtatctt gtgcatagat gaccactaag
    10921 atcgaaacgg gacgcttata cactttatcc ttcacgctgc ctcttcgttg actgccttcg
    10981 ctcatcccat tcacatagtt atctatgctc atgggagttc actcagttgc cgccttgatg
    11041 caacgcgaat gatttcgtgt atttgagtca tatcagttat tctcagcctt cttcacaacc
    11101 tgaatgctca gctcagccag tacagtcggg ttagcaaagc tcggtgcttc agtcatcaga
    11161 cacgctgccg ccgtggtttt cgggaaggcg ataacgtcac ggatattgtc ggtgccagtc
    11221 agcagcatgg tcagacggtc aagaccgaat gccagacctg cgtgcggcgg agtaccgtat
    11281 ttcagggcgt cgagcaggaa gccgaatttc tcgcgctgtt cctcttcgtt gatacccaga
    11341 ataccaaaca ccgtctgctg catatcacca ttatggatac gtactgaacc accgcccact
    11401 tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg cattttccgg tgcagctttc
    11461 agttctgcag cagtcatgtc tttcggtgag gtgaacggat ggtgcattgc cgtcaggccg
    11521 ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc acagcggtgc ccatttgctt
    11581 tcgtcggtca gaccaaggtc tttacccact ttcaggcgca gtgcgcccat cgcgtcggca
    11641 acaattttct tgttgtcggc accgaagaaa atcatatcgc catcttgcgc ggcagtacga
    11701 tccaggatgg cttcgatgat ttctgcatta aggaacttcg ctaccgggct gttgatacct
    11761 tccagacctt tcgcgcgttc gttaactttg atgtaagcca gacctttcgc gccgtagatt
    11821 ttaacgaagt taccgtattc gtcgatctgc ttacgggtca gcgatgcgcc acccggaaca
    11881 cgtagagcgg caacacggcc tttcggatcg ttcgccggcc ccgcaaatac tgcaaactca
    11941 acagatttca gcagatcggc aacgtcggtc agttccatcg ggttacgcag atccggttta
    12001 tcagaaccat aacggcgttc tgcttccgca aaggtcatta ccgggaaatc gcccagatcc
    12061 acgcccttca cttccagcca caaatgacgc accagcgctt ccatcacttc acgcacttgc
    12121 ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa attcaggctg acgatcagcg
    12181 cgcaggtctt cgtcacggaa gcatttaacg atctgatagt aacggtcaaa accggacatc
    12241 atcagcagct gtttgaacaa ctgtggggat tgcggcagtg cgtagaattt acctttgtgc
    12301 acacgagaag gcaccaagta gtcacgcgcg ccttccggcg tggctttggt cagcatcgga
    12361 gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc gcaccaggct ggtgatttta
    12421 gcgcgggttt tcaggcgctg cgccatttcc gggcgacgca ggtcgaggta gcggtatttc
    12481 agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg gcagaacatc tgcgcggttg
    12541 atgatagtca gcgaggacgc cagcacttca atttcgccgg tcgccatatc gcggttaata
    12601 tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa tgcagaactc attacgcagt
    12661 tcagaggcca gctttaacgc gtccgcacga tccggatcga aaaatacctg cacgatacct
    12721 tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat cacgacgacg gttgacccaa
    12781 ccacacagag tcacctgctg ccccacgtgg gacaaacgga gctgtccaca atattctgta
    12841 cgcatgagat atcccttaac ttagctgccg gcggatgccc cctgctgcgc aggtgaccaa
    12901 gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag gcggctatta tactggaaat
    12961 tctgccgcac cgtaagagcc tggcccgcgc tgtaacgcct cgttaccact ttatatcggg
    13021 cctgaaatca gactctacgc cagtttgcta taaaggtgtt gcccgaactc ataaaaatta
    13081 acaaaatttg tcgttccgcc atcggctaat cgcattaagg tgtgaggcac gattttgttt
    13141 tttcaggagt cagcatgctt gaacttaatg ctaaaaccac tgcgttggtg gtgatcgatt
    13201 tacaagaagg catcttacct tttgccggtg gtccacatac tgccgatgag gtagttaatc
    13261 gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca gcccgtgttt ctggtgcgag
    13321 ttggctggtc tgccgattac gccgaagcat taaaacagcc ggttgatgcc ccctcccccg
    13381 ctaaagtgtt gcccgaaaac tggtggcaac atccagctgc attaggtgca actgacagtg
    13441 atatcgaaat catcaaacgt cagtggggtg cgttttacgg tacggatctg gagttgcaac
    13501 tgcgccgccg aggtatcgat acaatagtgt tatgtgggat ctcgaccaat atcggtgttg
    13561 aatccaccgc ccgcaatgcc tgggaacttg gctttaatct ggtgattgcc gaagacgcct
    13621 gtagtgccgc cagcgccgag caacacaata acagcattaa tcatatctac ccgcgcatcg
    13681 cccgtgtgcg tagcgtggaa gagatccttc acgcgttatg atttacatcg gtctaccgca
    13741 atggtcgcat cctaaatggg tgcggttggg gatcaccagc cttgaagagt atgcccgcca
    13801 ctttaactgc gtgatacggg atttttaaaa atcactaaag aacgcccaag agcatgtgtt
    13861 ttctttagaa taatcaaagc gttggattgc catgccatat gaaaatcggc aaagttcata
    13921 tgtaacaaag tgtcccaatc atgccccaaa atgccccaca acaaatattt ttgccccatg
    13981 catgccccaa aaaatcactt tccctcactc ttgacactgt ttatccatac agttaaaaat
    14041 aatactgtat aaaaacacag tgtagaggga tttttatgcg tattgaaatc tgcatagcca
    14101 aagagaaaat gactaaaatg ccaaccggtg ctgtggatgc gttaaaggaa gaattaaccc
    14161 gacgcatcag taaacgttat gacgatgtag aggtgatcgt aaaagccacc agcaacgatg
    14221 gcctttctgt tacgcgcacc gccgataaag attcagctaa aacttttgtt caggaaactc
    14281 tgaaagatac ctgggagtct gctgacgagt ggtttgttca ctaattagca cgtaaaatca
    14341 gtaacggctg gaaatcattc aatactcgca ctatcggaag ttcaccagcc agccgcagca
    14401 cgttcttgca tacgacgtgt ctgcggtctc aactccgccc gcccccgcca atcatgattg
    14461 ggcagtacca gtacagcact agcaaagaca ggaaattagc agtctcagca aaacaccgac
    14521 cagacggtga gaagacaaaa agatacgcaa agatgccgcg gttccagaag aataaaaaac
    14581 cgctaatgga cagcctcctt aacacagttt tttatataca tgattcactg ataccagtgg
    14641 cattaatagc tattatatag cttcagctcc ttttttcata ccttaaggaa aaattgcccc
    14701 ctcccccaaa aagtatcgtt tttttacatt aaaccgatcc agtttaggat agtgtaaagc
    14761 agaagttctt caatatataa catatggagt taatatgctt acacaaacta tatattcgcc
    14821 tcctccagaa gttcctgtct actgctgaag ggaaccaata acattgcaaa gcagataaaa
    14881 aagaatccaa cacctctcaa cataaagaat acgccctgat atttatgctc ccgacaaata
    14941 acaatcacag attaatttca aattcgttct ccacttattc aatcgacact agccgtgcat
    15001 atgaaaatta tctaacccat tggactgaat ggaaaaataa ccgcatacaa gaagaacaac
    15061 gagacatcgc ttttcagcga ctagtatcat gtctacaaaa ccaagagacg aacctggact
    15121 tgtctgaatt aggcctgaca acattacctg aaatcccccc ggaaattaaa tcaattaata
    15181 taagtaaaaa taatttaagc ttaatctccc cattgcctgc gtcccttaca cagcttaatg
    15241 tcagctataa cagacttatt gaactgcctg ctttgcctca aggacttaaa ttattgaatg
    15301 cgtcccacaa tcaactaatc acactaccca cactccccat atctttgaag gagcttcatg
    15361 tctcaaataa tcaattatgt tctcttcctg ttttaccaga actactggaa acattagatg
    15421 tatcatgtaa tgggctggca gttttaccac ctttaccatt ttctttacaa gagattagcg
    15481 caatagggaa tcttcttagt gaactccccc ctctacctca caacattcac tccatatggg
    15541 caatcgacaa tatgttaacc gatattccat acctgccgga aaatttaagg aacggttatt
    15601 ttgacataaa tcagataagt catatcccgg aaagcattct taatctgagg aatgaatgtt
    15661 caatagatat tagtgataac ccattgtcat cccatgctct gcaatccctg caaagattaa
    15721 cctcttcgcc ggactaccac ggcccgcaga tttacttctc catgagtgac ggacaacaga
    15781 atacactcca tcgccccctg gctgatgccg tgacagcatg gttcccggaa aacaaacaat
    15841 ctgatgtatc acagatatgg catgcttttg aacatgaaga gca
//
LOCUS       DAJTVK010000095        15584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000095 DAJTVK010000000
VERSION     DAJTVK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.095"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2067
                     /locus_tag="OO372_003021"
     CDS             <1..2067
                     /locus_tag="OO372_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6342453.1"
                     /translation="SGYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNS
                     GLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSV
                     RFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITD
                     LQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHS
                     DFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGD
                     VFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYD
                     IADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLR
                     RISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFAS
                     NNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQA
                     GASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMT
                     PYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQP
                     LTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDES
                     RNYICQ"
     gene            2083..3117
                     /locus_tag="OO372_003022"
     CDS             2083..3117
                     /locus_tag="OO372_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCR6342454.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="OO372_003023"
     CDS             complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="OO372_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6342455.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="OO372_003024"
     CDS             complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="OO372_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR6342456.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4701..4973
                     /gene="rcnR"
                     /locus_tag="OO372_003025"
                     /pseudo
     CDS             4701..4973
                     /gene="rcnR"
                     /locus_tag="OO372_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5065..5161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5196..5984
                     /gene="thiM"
                     /locus_tag="OO372_003026"
     CDS             5196..5984
                     /gene="thiM"
                     /locus_tag="OO372_003026"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6342457.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5981..6781
                     /gene="thiD"
                     /locus_tag="OO372_003027"
     CDS             5981..6781
                     /gene="thiD"
                     /locus_tag="OO372_003027"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6342458.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6846..7664
                     /locus_tag="OO372_003028"
     CDS             6846..7664
                     /locus_tag="OO372_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6342459.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7716..8462
                     /locus_tag="OO372_003029"
     CDS             7716..8462
                     /locus_tag="OO372_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6342460.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8436..9401)
                     /locus_tag="OO372_003030"
     CDS             complement(8436..9401)
                     /locus_tag="OO372_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6342461.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9398..10402)
                     /locus_tag="OO372_003031"
     CDS             complement(9398..10402)
                     /locus_tag="OO372_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR6342462.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10399..11676)
                     /locus_tag="OO372_003032"
     CDS             complement(10399..11676)
                     /locus_tag="OO372_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6342463.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11933..12985
                     /gene="fbaB"
                     /locus_tag="OO372_003033"
     CDS             11933..12985
                     /gene="fbaB"
                     /locus_tag="OO372_003033"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6342464.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13304..14158
                     /gene="gatY"
                     /locus_tag="OO372_003034"
     CDS             13304..14158
                     /gene="gatY"
                     /locus_tag="OO372_003034"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6342465.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14187..15444
                     /gene="gatZ"
                     /locus_tag="OO372_003035"
                     /pseudo
     CDS             14187..15444
                     /gene="gatZ"
                     /locus_tag="OO372_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15454..>15584
                     /locus_tag="OO372_003036"
     CDS             15454..>15584
                     /locus_tag="OO372_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6342466.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4089 a         3728 c         3988 g         3779 t
ORIGIN      
        1 agtggctatg ttccaccgga aaactgggag cggggtatta atgcgtttta tacctcttat
       61 tatgtgagtc agtattacag tgactataaa gcgtcgggta atagtaagag tacatatgta
      121 cgttttaaca gcgggttaaa tttactgggg tggcaactgc attctgatgc cagtttcagt
      181 aaaacaaata acaatccagg ggtgtggaaa agcaataccc tgtatctgga acgtggattt
      241 gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca catcaagcga tatttttgat
      301 tctgttcgct tcagaggtgt gcggttgttt cgtgatatgc agatgttgcc taactcgaaa
      361 caaaatttta cgccacgggt gcaggggatt gctcagagta acgcgctggt aactattgaa
      421 cagaatggtt ttgtggttta tcagaaagag gttcctcctg gcccgtttgc gattacagat
      481 ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg tgaaagaggc ggacggttcg
      541 gtaaccacct atctggtgcc ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg
      601 aaatatgatt ttgcggcggg tcgtagccat attgaagggg cgagcaaaca tagtgatttt
      661 gtccaggcgg gttatcagta tggttttaat aacttattga cgttgtatgg tggctcgatg
      721 gttgcgaata attattacgc gttcactttg gggactggct ggaatacacg cattggtgcc
      781 atttccgtcg atgccacgaa gtcgcatagt aaacaagaca acggcgatgt gtttgacggg
      841 caaagttatc aaattgccta caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg
      901 gcggcctggc gttattcgtc gcgtgattac cggacattta acgatcacgt ttgggcaaac
      961 aataaagata attatcgccg tgatgaaaac gatgtctatg acattgccga ttattaccag
     1021 aatgattttg gtcgcaaaaa tagcttttcc gccaatatga gccagtcatt gccagaaggt
     1081 tgggggtctg tgtcattaag tacgttatgg cgagattact gggggcgtag cggcagtagt
     1141 aaggattatc agttgagtta ttccaacaac ctgcgacgga taagctatac cctcgcggca
     1201 agccaggctt atgacgagaa tcatcatgaa gagaaacgtt ttaatatttt tatatcgatt
     1261 ccctttgatt ggggtgatga cgtttcgacg cctcgtcggc aaatatatat gtctaactca
     1321 acgacgtttg atgatcaggg gtttgcctca aataatacgg gattatcagg aacagtaggg
     1381 agtcgggatc agttcaatta tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg
     1441 acagctggag cgaatttaac ctggaacgcg ccggtggcaa cagtgaatgg cagttatagt
     1501 cagtcgagta cttatcgaca ggctggagcc agtgtttcag ggggcattgt cgcctggtcg
     1561 ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga
     1621 attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg
     1681 gtatacgacg gaatgacacc ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc
     1741 gatagcgaag cagaattacg tggcaaccgg aaaattgccg caccttatcg cggcgcggtt
     1801 gtactggtta attttgatac cgatcagcgc aagccctggt ttataaaagc gttaagagcg
     1861 gatgggcaac cattaacgtt tggttatgaa gtcaatgata tccacggtca taatattggc
     1921 gttgtcggcc agggaagcca gttatttatt cgcaccaatg aaataccgcc atcggttaat
     1981 gtagcaattg ataagcaaca aggactttca tgcacaatca ccttcggtaa agagattgat
     2041 gaaagtagaa attatatttg ccagtaatag tcaggtggtt ctatggaaat tcgcataatg
     2101 ctatttatat taatgatgat ggttatgcct gtgagctatg cggcatgtta tagtgagtta
     2161 tctgttcagc acaatttggt tgttcagggg gattttgcac ttactcaaac acaaatagcg
     2221 acatatgagc ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg
     2281 agttcgtcgg atattattgt tgggctttat aacgatacca taaaattaaa tttacatttt
     2341 gaatggacca ataaaaacaa catcacgttg tcaaataatc agaccagttt caccagtggt
     2401 tattcagtta cggtgacacc tgcggccagt aatgcaaaag tgaatgtttc tgcggggggc
     2461 ggcggttcag tgatgattaa tggtgttgcg acattatcca gtgcttcatc atcgacacgc
     2521 gggagtgccg cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt
     2581 gtaaatagct acagaaatgc attggcacaa aatgcaggtg tctattcctt taatctgaca
     2641 ttgtcataca acccgataac cacaacctgc aaaccggacg atttattaat tactttagac
     2701 agtattcccg tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaacaa
     2761 ggggatatta ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa
     2821 atgcaggtgt atttatcaag ttctgatttg ttagccggca gtaacacgat cctgaaaggt
     2881 gcagaaaata atggagtcgg atttatgctt gaaagcaatg gttctccagt cacattttta
     2941 aatatcactg ataacagcaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa
     3001 cttacagata caacggtttc aattccgata actgccagtt actacgtcta cgatacaaac
     3061 aaagttaaat ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa
     3121 caagcaagac gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc
     3181 atgatgaggg ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat
     3241 tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc
     3301 ggcatcaggc gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg
     3361 gtacatctct gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact
     3421 taactcatac ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca
     3481 ggcggcactg gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa
     3541 ctctccactt tggggatctg taatatcagt cgcatatctt tcctgccata caatataact
     3601 gacaagcgat attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc
     3661 cctaaaagca tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct
     3721 gggctgaaga taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca
     3781 tgtacgccca tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt
     3841 ttagcgagag tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg
     3901 ccaacgccta cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt
     3961 gcgcccagtg tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg
     4021 caggggataa ggccaccggt taagccaaat agcaaaattt gccagttggt gacctctcta
     4081 ccatcaaagc gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg
     4141 ccatgttcgt gatggtgatg atgtccatgg tcgtggtgat cttcgtgatc gtgatgatga
     4201 tgctcataat catactcgtg catattctcc agccagttgc gttcgccgcg ccaggtacgc
     4261 cagaacatcc agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt
     4321 tctgctgatt gagcagtaaa gcgcttgctg atcaccatcc cgccaaaggc aattaaccag
     4381 accactgcgg tatgcgaaat agttgctgcc agtccgagca tcaccgcttg tttaatggtg
     4441 cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca
     4501 tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga
     4561 agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat ctgattctac
     4621 tccccccagt acctgatact accccccagt agattaatag tgctatgata ttttcatatt
     4681 cttgttaacc aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc
     4741 gtgccagtaa gattcagggc taggtcgtgg cgctcaagaa aatgctcgac gagccgcatg
     4801 aatgcgctgc agttttacaa cagattactg ctatccgtgg cgcggtaaac ggtctgatgc
     4861 gggaagtgat taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac
     4921 gtgaagaaga tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta
     4981 acgcgattgt aaaactgccg tttttcctcg tttacaacgc gtgcgctgga cattaccatc
     5041 ctcctctgcg atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg
     5101 agaaataccc gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag
     5161 gtcattgctt cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgggttcag
     5221 cgcaatctgc gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga
     5281 ccaatgatgt ggtgcaaacc tttaccgcta ataccttgct ggcgctcggt gcatcgccag
     5341 cgatggttat cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga
     5401 ttaacgttgg cacactgacg cagccacgcg ctcaggcgat gcgtgctgcc gttgagcaag
     5461 caaaaagctc tcaaacaccc tggacgcttg atccagtagc ggttggtgcg ctcgattatc
     5521 gccgccattt ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt
     5581 cggaaatcat ggcattagct ggcattgcta atggtggacg gggagtggat accactgacg
     5641 ccgcagctaa cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg
     5701 tggtcactgg cgaggtggat tatgttaccg atggccatcg cgccgttggc attcacggtg
     5761 gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg
     5821 cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga
     5881 aacaagccgg agagcgcgca gtcgccagaa gcgaggggct aggcagtttt gttccacatt
     5941 tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct
     6001 gacgattgcc ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt
     6061 ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg
     6121 tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt
     6181 gttcagcgat gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt
     6241 tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt
     6301 tatgctggca aaaagcggcg acccactgct ttcaccttcg gcagttgcca cgctacgcag
     6361 tcgattattg ccacaggttt cattaataac gccaaacttg cccgaagcag ccgccttgct
     6421 cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc
     6481 gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga
     6541 ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa
     6601 cacccacggt actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac
     6661 aaactgggct gacaccgtac aggaggcaaa aagctggctt tcatcggcgt tagcccaggc
     6721 cgacacgctg gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg
     6781 agcaccgtac tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga
     6841 aaccaatgca gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg
     6901 ggctaatttc catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag
     6961 cagttcagat tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta
     7021 ttcgtattcc tgccagttac accattcacg gaatagatgt ttcgcgctgg caggagcgga
     7081 tcgactggca gcgtgtggca aaaatgcgcg ataacggtat tcgcttacaa tttgccttta
     7141 ttaaggcgac ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaactaa
     7201 gccgcgaaaa tggcttgctg cgcggggcat atcattattt ctccccgtcg gtagcagcac
     7261 cggttcaggt gcgattattt ctgcaaacgg tagatttctc acaaggcgat ttccctgcgg
     7321 tactggacgt agaagagcga ggaaaattat cggcaaaaga attacgcaag agggtaagtc
     7381 agtggctaaa aatggtcgaa aaaagtacgg ggaaaaagcc gattatttac tcaggagccg
     7441 ttttttatca cacgaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact
     7501 actatcaacg tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc
     7561 gtggacaggt agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacggtgg
     7621 aggagttgca gggattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata
     7681 tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga
     7741 ttgcccaact gaacgaacgt attttagcgg cggataacac gccgctttat attaagtttg
     7801 ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg
     7861 gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc
     7921 aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca
     7981 acatcttcga atactcgttg aaagaagcgc gaggtttttc tcagcaggtg gtgttgcgcg
     8041 ggaaaaagcc cgatacgcta tgggttaaca agcgtgtcgt gaaatgtccc gaagaagtcg
     8101 cgcagcagct ggcggtcgaa gcaggaagtg atgtcttttt gcttaagcgt attcgctatg
     8161 tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg
     8221 ttgatgccat cgggatttca ctttatgact atttccgcag ccagcacatt tacccacaac
     8281 gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt
     8341 tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc
     8401 ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt
     8461 agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact
     8521 gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg
     8581 ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga
     8641 atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc
     8701 aacggcgcgg caaatttctc ctgccattgc tcgccaagtg ttgttatctc tgcggataaa
     8761 gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gcgacactaa aggtcgacag
     8821 gccatgatcc gcgccagtaa tgcatccggg atatcgccaa tacgtgggcc aaaatcgata
     8881 aacggcgtca cgtcttgcag ctcttccagc cattccacta acaattcgcc gcagggcgag
     8941 gccagttgat aaccggaaaa atagagcagg ctgccaggcg caacggttaa tcgcgccagc
     9001 cactggcgat tccactgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc
     9061 ggctcaacca gcgccagaca ccagccgtta tcaccttcgg cgttatcgat caggctgatt
     9121 aagccctctt ttgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc
     9181 aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag
     9241 ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca
     9301 taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg
     9361 gttaattcag gcagcagcgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac
     9421 gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagtt
     9481 ccgcctttaa cgcaggatcg atagcgttaa cgccatgcaa cgcgccgcaa attgccgtcg
     9541 ccatagcacc gatggtgtct gtgtcgccgc caaggttagc gcacaggacg gcgcagcgat
     9601 tcgggtcggt ttgtgctagt tcaaccagcg caatggcgca cggaacggac tcaatagtgc
     9661 tggtacctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat
     9721 tgcgcacaat tttcagcgca atctccagcc gcgccgccag tgaggcgctg aacgtagtga
     9781 tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaggga atcaacaatc gctgaccagc
     9841 tttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca
     9901 gatcggattt atgggtcggg ctggacgcca gcgctacatc atcaataaag gaatcaacat
     9961 cacgcgccgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggtcacgc
    10021 cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg
    10081 aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg cagcgcccag tcgagaatat
    10141 tacgcccaat cagatccgga tcgatcttgc cttcacgttc cagtaacgca tccgccagac
    10201 acagcgccat cgaggtatcg tcggtgaatt cggcgcggtt aaaataacag gccgcgttat
    10261 tctcctttgg tccaggaaga aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc
    10321 gtggccaaag ctcggagggc atccccatcg catcccctaa cgcctgccca taaagagcac
    10381 cgagaatacg ttctgttttc atttaacttc cccttgtgtc aacgcaatat cgcgatcatc
    10441 gaccttgata gccgtaattt cgttgtcgga ttcgcggaaa aaaatcatga acagcacggc
    10501 gataatggca atcatcaccg cgccgaaagt ccacatcccg gaccagttga aagtcagtcc
    10561 gtttaccggt tcctgataag cgaacatctt ttccatcatc acaccgccaa gacgatagcc
    10621 gagcaaactg ccgaagccct ggcagcagag cgtgatcagc ccctgcgcag cggtacgcat
    10681 atgcacgggg gcttttttat cgacatagat gtaagcggta acgtagtaaa aatcgtaact
    10741 tacgccgtga agcaaaatac cgaggaacag taacgcgtag gtgaaatatt catccgtact
    10801 accgtaaata aagaagccat agcggatcgc agcggtgacc agaccaagca ataatacctt
    10861 tttgatacca aagcgtttag tgaaaaacgg caatgccagc ataaagaaga tttcagagaa
    10921 ctggccgagc gtcatccagc cggtggcgtt tttcatgcca acttcggtca gataaccgtt
    10981 ggcaaagatg taatagaacg ccagtggcat cgcaaacagg aatgaacaga agaaaaagac
    11041 gaggaagttt ttatcgcgca gcaggatcag cgcatccagg ccgagcatga ctttaatatc
    11101 cattttgccg gtgctttttg gtggcgtgtc gggcaggaaa aacgcaaaca caccgagcag
    11161 agcagaactt ccggcggtaa tcagcagcgg gatgttagtc ggtgagatat cggcataccc
    11221 cagtatttgc ggcaagaaac cacatgccag accggaggca atccagccga tagtgcccat
    11281 cacacgaatg cgcgggaaat cacgctccac atccggcacg ttggcaaaag cgatgctgtt
    11341 agtcagcgca atggtcggca tataggttag agagtaagcc agcagtaacg gaaagaaccc
    11401 ggcaaaagtg gtctgttgcg cagcgaaata catcagcagc gcgcctgcga acatcaatac
    11461 cgccagcact ttttgcgccg agaaaaagcg gtcagtgatg gagccaacca gaattggcga
    11521 caggatcgcc gcaatggcgg tacaggcata cgaccagcca atttctccgg cactaaaacc
    11581 gcttttactt aaccacaacc acaatggcac aaaccacgcg ccccagataa accattcaac
    11641 aaacatcatg aacgacagct ttgctgttgt tttcatcttt taattccttc atgacaggta
    11701 atgtaaggta cgcgatgaca ataccactta acaatacctt ttaaatacct ttgcggtgaa
    11761 aacttgaccg cgctaacatt ttttctgatg aatcgagcca acagaaaacg ctgaaaaaac
    11821 atccaaaaga tggaaaaact cgcccgtaac gaccaggatt atgattgccc gcaaaaggcg
    11881 ggccaggaca atccatagcc gatatccaat cggaatttac gggagcatag taatgacaga
    11941 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgacaat
    12001 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtaa tgattgacaa
    12061 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    12121 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    12181 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    12241 agcgggctgt aactgtgtgg cgtcaactta cggcgtgttg gcgtcggtat cgcggcgcta
    12301 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    12361 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    12421 ggttggtacg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    12481 tgcggccttt gaacgcgcgc acgagctggg catggtgacc gtgctgtggg cctatttgcg
    12541 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgaccggtca
    12601 ggcaaaccat ctggcggcaa ccatcggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    12661 taacggcggc tataaagcaa ttaattacgg ttacaccgac gatcgtgttt atagcaaatt
    12721 gaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    12781 ggctgggttg ataaactccg gcggtgctgc gggcggtgaa actgacctca gtgatgcagt
    12841 gcgtactgcg gttatcaaca aacgcgctgg cggaatgggg ctgattcttg gacgtaaagc
    12901 gttcaagaaa tcgatggctg acggcgtgaa actgattaac gccgtgcagg acgtttatct
    12961 cgatagcaaa attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg
    13021 gataaggcgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    13081 catgcctaca tagcactgcc acgtatgtcg gaaaaggcgt ttacaccgca tccggcataa
    13141 aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc ctttcactcc
    13201 tcgaacagtt ttcatattgt cgttttgtga tcgttatctc gacatttaaa aacaaatcat
    13261 ttcattatat tttgaaatcg aaaacaagca acaggatatg aaaatgtacg tggtatcgac
    13321 aaagcagatg ctgaacaacg cacagcgcgg cggttatgcg gttccggcat tcaatattca
    13381 caatctcgaa acgatgcaag tggtggtgga aaccgctgcc aacctgcatg cgccggtcat
    13441 catcgccgga acgcctggca cattcactta tgctggtaca gaaaatctgt tggcgctggt
    13501 cagcgcgatg gcgaagcaat atcaccatcc actggcaatt catctcgacc atcacacgaa
    13561 atttgacgat atcgctcaga aggttcgttc tggcgtgcgc tcagtcatga ttgacgcctc
    13621 gcatttgcct tttgcgcaaa atatttcacg ggtcaaagag gtggtggatt tttgccatcg
    13681 ctttgatgtc agcgtcgaag cggagctggg gcaacttggc ggccaggaag atgatgtgca
    13741 agtcaatgaa gccgatgttt tttataccaa ccccgctcag gcgcgtgaat ttgccgaggc
    13801 aaccggaatt gattccctgg cggtcgccat cggcacggct catgggatgt atgccagcgc
    13861 accggcgctt gatttttctc gactggagaa cattcgccag tgggtgaact taccgctggt
    13921 gctgcatggc gcgtcagggt tatcgactaa ggatattcag caaaccatca aactggggat
    13981 atgcaaaatc aacgttgcaa cggagctgaa aaatgccttc tcgcaggcgt taaaaaatta
    14041 cctgaccgag caccctgaag cgaccgatcc ccgggattat ttgcagtcgg ctaaatccgc
    14101 aatgcgcgat gtggtgagca aagtgattgc cgattgtggc tgcgagggca gggcataacg
    14161 cacctgccat ttaacaagga aaaaacatga aaacgttaat tgcccggcat aaagctggtg
    14221 aacatatcgg catatgttca gtctgttctg cccatccgtt ggttatcgaa gcggcgctgg
    14281 catttgatcg caacagcacg cgcaaagtgc tgattgaagc aacgtcaaac caggtcaatc
    14341 aatttggcgg ttataccgga atgacaccgg cagactttcg cgaatttgtt tttacgattg
    14401 ccgataaagt cggatttgca cgcgaacgca ttattctcgg cggcgatcat ctggggccaa
    14461 actgctggca gcaagaaaat gcggatgcgg cgatggaaaa atccgtcgag ctggtaaaag
    14521 catatgttcg tgctggcttc agtaaaattc atcttgatgc gtcaatgtcc tgcgcggggg
    14581 atcccatacc gttagcacca gaaacggttg cggaacgagc tgctttgctt tgctgcggaa
    14641 agtgtggcga cagattgcca gcgtgagcaa ctgagctatg tcattggcac tgaagttccg
    14701 gttccgggcg gtgaggccag cgccattcag tcagtacaca tcacccatgt tgaagatgcc
    14761 gccaatactt tacgtacgca tcaaaaggcc tttattgccc gtgggctgac agaggcgtta
    14821 acacgcgtga ttgccatcgt ggtgcagccg ggcgtggaat ttgatcacag caatattatc
    14881 cattatcagc cgcaggaagc gcagccgctg gcgcaatgga tagaaaacac ccgaatggtt
    14941 tatgaagcac attctaccga ttaccagacc cggacggctt attgggaatt agtccgcgat
    15001 cactttgcaa tattgaaagt cggtcccgca ttaacctttg ctttacgcga ggcgatattt
    15061 gcactggcac aaattgagca ggaacttatc gcccctgaaa atcgcagcgg ttgcctggcg
    15121 gtaattgaag aagtgatgct cgacgaaccg caatactgga aaaaatatta tcgtacgggt
    15181 tttaacgatt cattactgga tattcgttac agcctgtcgg atcgtattcg ttattactgg
    15241 ccgcatagtc ggattaaaaa tagcgtcgaa acgatgatgg tgaatcttga aggcgtggac
    15301 atcccactgg gcatgattag tcagtatctt cccaaacaat ttgaacgcat tcagtccggg
    15361 gaattatcag caataccgca tcagctgatt atggataaaa tttatgatgt tttgcgcgcc
    15421 tatcgctacg gctgtgcgga ataaggacgg tatatgacta acctgtttgt tcgtagcgga
    15481 atttcttttg tcgatcgtag cgaagtttta acccatatcg gtaatgagat gctcgccaaa
    15541 ggtgtggttc atgatacctg gccacaggca ttaattgcca gaga
//
LOCUS       DAJTVK010000096        15406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000096 DAJTVK010000000
VERSION     DAJTVK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.096"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            10..453
                     /locus_tag="OO372_003037"
     CDS             10..453
                     /locus_tag="OO372_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6342467.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="OO372_003038"
                     /pseudo
     CDS             669..758
                     /locus_tag="OO372_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="OO372_003039"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="OO372_003039"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6342468.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="OO372_003040"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="OO372_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6342469.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="OO372_003041"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="OO372_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6342470.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="OO372_003042"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="OO372_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6342471.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="OO372_003043"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="OO372_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6342472.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="OO372_003044"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="OO372_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6342473.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="OO372_003045"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="OO372_003045"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6342474.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="OO372_003046"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="OO372_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6342475.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="OO372_003047"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="OO372_003047"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6342476.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="OO372_003048"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="OO372_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6342477.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="OO372_003049"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="OO372_003049"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6342478.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="OO372_003050"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="OO372_003050"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6342479.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="OO372_003051"
     CDS             13055..13435
                     /locus_tag="OO372_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6342480.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="OO372_003052"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="OO372_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6342481.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="OO372_003053"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="OO372_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6342482.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="OO372_003054"
     CDS             complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="OO372_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6342483.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3917 a         4043 c         3850 g         3596 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt taaaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaatg taacggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaaa
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAJTVK010000097        15235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000097 DAJTVK010000000
VERSION     DAJTVK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.097"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..749
                     /locus_tag="OO372_003055"
     CDS             <1..749
                     /locus_tag="OO372_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR6342484.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="OO372_003056"
     CDS             complement(790..1722)
                     /locus_tag="OO372_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6342485.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="OO372_003057"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="OO372_003057"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6342486.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="OO372_003058"
     CDS             complement(5254..5886)
                     /locus_tag="OO372_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6342487.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="OO372_003059"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="OO372_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6342488.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="OO372_003060"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="OO372_003060"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6342489.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="OO372_003061"
     CDS             complement(8729..8839)
                     /locus_tag="OO372_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6342490.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OO372_003062"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="OO372_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6342491.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OO372_003063"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="OO372_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6342492.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OO372_003064"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="OO372_003064"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6342493.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="OO372_003065"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="OO372_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6342494.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3914 g         3553 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttactt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgct
//
LOCUS       DAJTVK010000098        15084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000098 DAJTVK010000000
VERSION     DAJTVK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.098"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /locus_tag="OO372_003066"
     CDS             <1..781
                     /locus_tag="OO372_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342495.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="OO372_003067"
     CDS             complement(984..1337)
                     /locus_tag="OO372_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6342496.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO372_003068"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="OO372_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6342497.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="OO372_003069"
     CDS             complement(3165..3458)
                     /locus_tag="OO372_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6342498.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="OO372_003070"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="OO372_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6342499.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO372_003071"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="OO372_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6342500.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="OO372_003072"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="OO372_003072"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6342501.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="OO372_003073"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="OO372_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6342502.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="OO372_003074"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="OO372_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6342503.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="OO372_003075"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="OO372_003075"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6342504.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="OO372_003076"
     CDS             10838..11413
                     /locus_tag="OO372_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6342505.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="OO372_003077"
     tRNA            11520..11595
                     /locus_tag="OO372_003077"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="OO372_003078"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="OO372_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="OO372_003079"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="OO372_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCR6342506.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAJTVK010000099        14965 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000099 DAJTVK010000000
VERSION     DAJTVK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.099"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="OO372_003080"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="OO372_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6342507.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="OO372_003081"
     CDS             complement(1608..1931)
                     /locus_tag="OO372_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6342508.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO372_003082"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="OO372_003082"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6342509.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO372_003083"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="OO372_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6342510.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO372_003084"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="OO372_003084"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6342511.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO372_003085"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="OO372_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6342512.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="OO372_003086"
     CDS             7531..8568
                     /locus_tag="OO372_003086"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6342513.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO372_003087"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="OO372_003087"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6342514.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="OO372_003088"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="OO372_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6342515.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO372_003089"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="OO372_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6342516.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="OO372_003090"
     CDS             10739..11620
                     /locus_tag="OO372_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6342517.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO372_003091"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="OO372_003091"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6342518.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="OO372_003092"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="OO372_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6342519.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="OO372_003093"
     CDS             complement(14017..>14965)
                     /locus_tag="OO372_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCR6342520.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3784 c         3922 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAJTVK010000100        14756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000100 DAJTVK010000000
VERSION     DAJTVK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.100"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="OO372_003094"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="OO372_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="OO372_003095"
     CDS             1909..3261
                     /locus_tag="OO372_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCR6342521.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="OO372_003096"
     CDS             3285..3767
                     /locus_tag="OO372_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCR6342522.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            3811..4766
                     /locus_tag="OO372_003097"
                     /pseudo
     CDS             3811..4766
                     /locus_tag="OO372_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4776..5255
                     /locus_tag="OO372_003098"
     CDS             4776..5255
                     /locus_tag="OO372_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342523.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKCNNEYISRKEDSAVK"
     gene            complement(5392..6177)
                     /locus_tag="OO372_003099"
     CDS             complement(5392..6177)
                     /locus_tag="OO372_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342524.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(6507..6583)
                     /locus_tag="OO372_003100"
     tRNA            complement(6507..6583)
                     /locus_tag="OO372_003100"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6547..6549),aa:Asp,seq:gtc)
     gene            complement(6716..7447)
                     /gene="dnaQ"
                     /locus_tag="OO372_003101"
     CDS             complement(6716..7447)
                     /gene="dnaQ"
                     /locus_tag="OO372_003101"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6342525.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            7512..7979
                     /gene="rnhA"
                     /locus_tag="OO372_003102"
     CDS             7512..7979
                     /gene="rnhA"
                     /locus_tag="OO372_003102"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6342526.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(7976..8698)
                     /locus_tag="OO372_003103"
     CDS             complement(7976..8698)
                     /locus_tag="OO372_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6342527.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            8732..9487
                     /gene="gloB"
                     /locus_tag="OO372_003104"
     CDS             8732..9487
                     /gene="gloB"
                     /locus_tag="OO372_003104"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6342528.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            9559..10917
                     /gene="mltD"
                     /locus_tag="OO372_003105"
     CDS             9559..10917
                     /gene="mltD"
                     /locus_tag="OO372_003105"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6342529.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(10966..11562)
                     /locus_tag="OO372_003106"
                     /pseudo
     CDS             complement(10966..11562)
                     /locus_tag="OO372_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(11593..12393)
                     /locus_tag="OO372_003107"
     CDS             complement(11593..12393)
                     /locus_tag="OO372_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6342530.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            12634..13548
                     /locus_tag="OO372_003108"
     CDS             12634..13548
                     /locus_tag="OO372_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6342531.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(13545..14348)
                     /gene="dkgB"
                     /locus_tag="OO372_003109"
     CDS             complement(13545..14348)
                     /gene="dkgB"
                     /locus_tag="OO372_003109"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6342532.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(14511..14587)
                     /locus_tag="OO372_003110"
     tRNA            complement(14511..14587)
                     /locus_tag="OO372_003110"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14551..14553),aa:Asp,seq:gtc)
BASE COUNT         4073 a         3480 c         3499 g         3704 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat
     3961 cagaacactc atcatgttat tctcttgaca cttgatgttg caaaaaaaaa aaaaaaaaaa
     4021 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa tactcagccc aataacatca tttgattaca
     4081 ttcattttat cactacacat ccttcaggta taaaagatac actcgcctgg ttagttaacg
     4141 cgagtaaatt aatgactgag tttgatgaca atgggaaaat tatatttaac attaatgcct
     4201 taaagtatac aaaagcaagt tactttgaaa tacttggaga gaaatatata aaaatcacaa
     4261 catccagccc ctggctttta gaaaagctcg gtaaatatat tttctcttca cgagcgcctc
     4321 aagttctgga actcgctatt ggctggcgag gagctttatc tgaatcaata aaaggagtta
     4381 agttctgtat ctggttttca gtagcctgga gaaccataga atttattatg agttcagagc
     4441 gagatctggt taattttctt ggcgattttt caatggatgt ggccaaagca gttatagccg
     4501 gtggtgttgc aaccgctatt ggaagcctgg cttcttttgc ctgtgttagc tttggctttc
     4561 cagtaattct tgtcggagga gcaattttac tgacagggat agtgtgtaca gttgttttaa
     4621 atgaaatcga tgctcaatgc catttatcag aaaaattaaa atatgcaatt agagatggac
     4681 taaaacggca acaggaactt gataaatgga aaagggaaaa catgactcca tttatgtatg
     4741 ttcttaacac tccacccgtg atatgaggat taaccatgcg tttacatgtt aaattaaaag
     4801 agtttctttc gatgtttttt atggccatat tatttttccc tgcattcaat gcttctttgt
     4861 tttttaccgg agttaaacct ctatattcaa taataaaatg ctccacggaa atattttatg
     4921 attggagaat gttgattctg tgctttggtt tcatgtcgtt ttccttttta aatattcatg
     4981 taattttact tacgataata aaattattct taatcaaaaa aacaaaggta gtcaatttcg
     5041 ccacagacat aacaatacag ctaactctta tatttttact aatagctatc gtaatagcac
     5101 ctttaatagc tccttttgtc acaggatatg tcaatgctaa ttatcacccc tgcggtaata
     5161 atacgggaat tttcccagga gcaatctata ttaaaaatgg catgaaatgt aataatgaat
     5221 atatttctcg caaggaagat tcagcagtta aataattaaa cattctaaaa caaacagttt
     5281 ttaactccgg attccatgtc ctttcttgca ctgtctttgt caagaaaaat ttcttatgca
     5341 aagaattttg tattatttcc gccagaaatt cctggcggca gatataattt attattgata
     5401 gcggttaacc aaatctttta ccatcgcttt ataggtcttt tgcttaagtt gcatgatttg
     5461 ctgctctgtc atatcagtcg tactggcttc attcttgtcg ggcaatggtg gaagcacttt
     5521 atattcataa ttcaccggcg accaggcagc ccactcacct gtagctacat ccaccagcgt
     5581 gaatcgtacc agatagcgca gtgacatcgt gtcagtcaat ttttgatttt tataatctga
     5641 ccaaactatt gatttcaatc cagagtcata ttttcctgtc tgcaacgtat cctggaaaac
     5701 aataatcgct ttctgatgcc cttttgcagc cacaaaacgc agtgcctgca tccagttcat
     5761 attctcagca ctggcaacat cctcgttctc atttttatct ttgtttttgt tacaagtcag
     5821 aggcttttgc ctgtccggga taccagagaa tgtggaaaca gtataatatt ttcgcatctc
     5881 ctcctgcata atggtttccg gtgcgcggtt gccggattga acaagtatta caggagaatt
     5941 aaggggaacg cggaactcat ctccatcaag cgcagcttga atatcctctt cggatacggc
     6001 cagagtggtt tcattaccga ataaatcttt gtctgtcagt tgctggggag tgtttttatt
     6061 aatattactt tgtgtgactt gagctttttt cttcttttca tcaatagaag attcagagac
     6121 acatcctgca agcagcgaga ataatacaca tatacaacaa acccttctta aattcataat
     6181 cggacactcc ctcgcctgtc ataatcatgg taatgtttag ctaacgattt ttaattatat
     6241 actccacttg aatcagaaca aacaaaaata acagtatata taaattcatt aaatcaatag
     6301 aaaaatgaaa atcataacat tcaaatatat ttaaattgaa tgatttgata gtatgattaa
     6361 atattaatga tatggtctac catatgtgat taacacattt acagcataac aaaaccatag
     6421 accgaagaaa tagcgggatt taaatgcaaa aaaaccgcaa aattaaaaat cttgcggttc
     6481 tctgaactca ttttcatgag tgaatttggc ggaacggacg ggactcgaac ccgcgccccc
     6541 ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc tccgcgttgt gttccgttgg
     6601 gaacgaggcg aatattacgg attgcctcac ccttcgtcaa cggtttttct catcttttga
     6661 atcgtttgct gcaaaaatcg cccaagccgc tatttttagc gccttttaca ggtatttatg
     6721 cccgccagag gcagcttccg cccttcttct ccaccagatc aagacgggct tcatgagctg
     6781 caagctcttc atctgtcgca aaaacaacgc gtaacttact tgcctgacgt acaatgcgct
     6841 gaattgttgc ttcaccttgt tgctgctgtg tctctccttc catcgcaaaa gccatcgacg
     6901 tttgaccacc ggtcatcgcc agataaactt ccgcaaggat ctgtgcatcg agtaatgccc
     6961 cgtgtagcgt tcgtttactg ttatctattt cgtagcgggc acataacgca tcgaggctgt
     7021 tgcgcttacc aggaaacatt ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg
     7081 tattggtctt cggaatatcg cgcttaagca acgaaaactc gtagtccata aagccgatat
     7141 cgaacgctgc gttatggatc accaactccg cgccgcggat atagtccatg aactcatcgg
     7201 ctacttcggc aaacgtgggc ttatcgagca aaaattcatc ggcaatacca tgtacgccaa
     7261 aggcttccgg atccaccagc cgatcgggtt tgagataaac atggaagtta ttgcccgtca
     7321 ggcgacggtt caccacttca acggcaccaa tctcaatgat cttgtggcct tcatagtgcg
     7381 caccaatctg gttcataccg gtggtttcgg tatcgagaac gatctggcgt gtaattgcag
     7441 tgctcatagc ggtcatttat gtcagactta tcgttttaca gttcgattca attacaggaa
     7501 gtctaccaga gatgcttaaa caggtagaaa ttttcaccga tggttcgtgt ctgggcaatc
     7561 caggacctgg gggttacggc gctattttac gctatcgcgg acgcgagaaa acctttagcg
     7621 caggctacac ccgcaccacc aacaaccgta tggagttgat ggccgctatt gtcgcgctgg
     7681 aggcgttaaa agaacattgc gaagtcattt tgagtaccga cagccagtat gtccgccagg
     7741 gtatcaccca gtggatccat aactggaaaa aacgtggctg gaaaaccgca gacaaaaaac
     7801 cagtaaaaaa tgtcgatctc tggcaacgtc ttgatgctgc cctagggcaa catcaaatca
     7861 aatgggaatg ggttaaaggc catgccggac acccggaaaa cgaacgctgt gatgaactgg
     7921 ctcgtgctgc ggcaatgaat cccacactgg aagatacggg ctaccaagtc gaagtttaag
     7981 cttgtggttt acgacattgc cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt
     8041 tactctgttt catcgggttt agcgttaaag gaatagtccg tttccgggca acaataagtt
     8101 gtaagcaacc aagcgcagga atatgcgcat tcaatagttt tcctccgtgt ttgttccacg
     8161 ggagaacgtg gaaacggctg gcgtgcagca cttcaaaatt caacaaagag agccagtcca
     8221 gttgccgcat cagagtaaac atccggctgt tatagggcga ggtttttcgc aataccggca
     8281 caagtttgcg taatcccatt aaactaatgg gattgaagcc actaatcacc agccagccat
     8341 catcaatcaa tacccgatcg gtttcacgca ataaacgatg cggatcggtg caccacggca
     8401 atgtatgtgc cagtagacaa acatcaacgg atttatcggc aaaaggaaga tgaagtggat
     8461 ccgcctgcac ctggacgggc attccttgcg cagaaacatt cacttgatga gaaaccgcgc
     8521 acgcttcgca attgatttct gcgcttaaat tgccaatctt aagcagatga aaaccataca
     8581 ttttagtgaa ccacgggttg agctggcgct ccagcgcctt gcgataaagc tctccccagg
     8641 gcaaatcgcc ccagcgatca ggagccacga cagtttgagg gactcttgcc ggtttcatca
     8701 caaccttccg tttcacacta agaggtaatc tatgaatctt aacagtattc ccgcctttga
     8761 tgacaattac atctgggttt tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg
     8821 agacgcagag ccagtattaa acgccattgc cgccaataac tggcaaccgg aggccatatt
     8881 tctcacccac catcatcacg atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc
     8941 gcaaattgtg gtgtatggtc cacaagagac acaagataag ggaacaacac aggtagtcaa
     9001 agatggcgaa actgccttcg ttttggggca tgaatttagt gtaattgcca caccgggtca
     9061 cactttagga catatctgtt acttcagtaa accttatcta ttttgcggcg acacgctgtt
     9121 ttctggtggg tgtggtaggt tgtttgaagg gacagcatca caaatgtatc aatcacttaa
     9181 taagttaagt gcgttacctg acgatacatt ggtatgttgt gctcatgaat atacgttatc
     9241 aaatatgaag tttgctttga gtattcttcc gcacgatttg tccataaatg attattatcg
     9301 taaagttaag gagttacggg caaaaaatca aataacacta cccgtaattc tgaaaaatga
     9361 gcggcaaatt aatgtttttt taagaacgga agatattgat ttaattaatg taattaatga
     9421 agaaacatta ttgcaacaac ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag
     9481 gttctgataa aactttcttg tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa
     9541 gcaactattg acacacacat gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg
     9601 ggttgccaga gtaccggcaa cgttcaacag cacgcacaga gcctttctgc agctggtcaa
     9661 ggggaagcag caaagtttac aagtcaggca cgatggatgg acgatgggac gtctatcgcg
     9721 ccagatggtg acttgtgggc tttcattggc gacgagctaa agatgggaat tccggaaaat
     9781 gaccggattc gcgaacagaa acagaaatat ttacgcaata agagctatct ccacgatgta
     9841 actttacggg cagagccgta tatgtactgg atagcagggc aagttaaaaa acgtaacatg
     9901 cctatggaac tggtactact acccatagtg gagagcgctt ttgatcctca cgcaacgtct
     9961 ggcgccaatg ccgcaggcat ctggcagatc attccgagca cggggcgcaa ttatggtttg
    10021 aaacagaccc gcaattatga cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg
    10081 aatatgatgc agcgtctgaa caaaatgttt gacggcgact ggcttctgac cgtagcggct
    10141 tataacagcg gcgaaggtcg ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa
    10201 tccacggact tctggtcgtt accgttgccg caggaaacga agcagtacgt gcctaaaatg
    10261 ctggcattga gtgatattct caaaaacagc aagcgttatg gcgtacgtct gccaacgacc
    10321 gatgaaagcc gtgctctggc gcgtgtgcac ctgagtagcc cggttgaaat ggcgaaggtt
    10381 gcagatatgg cggggatttc cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc
    10441 tccacgctgg gcgcaagtgg gccgcagtac gtgatggtgc caaagaagca tgcagatcaa
    10501 ctgcgtgaat ctctcgcttc aggcgaaatt gctgctgtac agtcgacgct ggttgccgac
    10561 aatacgccgc ttaacagccg tgtttacacc gtacgctctg gcgacacgct ttcaagcatc
    10621 gcttcacgtc tcggcgtaag caccaaagat ttgcagcagt ggaacaaact gcgcggatct
    10681 aagctgaagc caggccaaag tttgacgatt ggtgcaggca gtagcgcaca gcggttggca
    10741 aacaacagcg atagcattac gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct
    10801 aaacgccacg gcgtgaacat caaagatgtg atgcgctgga acagcgacac tgcgaatctg
    10861 caaccaggcg ataagctgac gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa
    10921 accagataat aaaaaaggca ccgattcccc cggtgccttt tttatttatg ccgctttatg
    10981 tgcttctacc atgatgatat cactggtgaa agagccatca gtctgcaagg aaaaatacgt
    11041 tctcacctct gtcgatgcgc tctgttggta aatgcgaata gcgtctacta acgcttctgg
    11101 cgtacgcatt ctcacgaccc atgaagaaaa ttccagcggt aacttatctg taattaaatt
    11161 atcaactatc agattggctt cattgattaa cgtcaaccac gcaccgctgg cgtagtttcg
    11221 tacgtgagag gtatcgcgta atgcttctac cgcctgtaac cagatgtcgc gcactgggtg
    11281 acccggagac attacatcca tcacaatcag cctaccgcca ggtttcaata tcctattcac
    11341 ttctcgcagt gctgcaccaa catcatgcca gtgatgggca gaataacggc tgataacaat
    11401 atcaaatgcg ttatcggcaa atggcagact tgcggcatat ccctggcggg tggtgatatt
    11461 tttcagttgc cgggcttcgg cggcttgtgc cacgacatcc agcatgtggg cagataagtc
    11521 atacgccacc accgcgctca cgttttgcgc agcgccaaac gttggtctgc cctgcggcag
    11581 acctgacata ccttatttat caggcttgcc gggactgaat tcaacgagta gcggattgtg
    11641 atcggaagcg cgcgtaacca gtacagaagc ttcactgacg ttcagaccac ggtagaaaac
    11701 aaaatcgagc gggcgaccaa acgcccgacg gcgctgatca tcggtaaaac gcacctggcg
    11761 cagcgacatt tcccgcgcaa agcgatataa cgcgttcatc cttctacggc tccaggcatt
    11821 gaaatctccc gccataatga ccgggccgct gtggtgagct atctgatcac caataggaag
    11881 taactgctta ctatagacat ccacgcccag actgaagttg acggcgtgta tattaaccac
    11941 catcaacagg cgggtgtcag gtaatggata gaccgtcacc agtgccgact tcgccagacg
    12001 caaaatgggt tctcgttcac gtaacgggca gcaatacact ggatgtgccg ccgaaagggt
    12061 cattacgcca gaaggatgtt gtggcagcac gaaagcgggt acctgatcgg cggcaagata
    12121 gttagcggtc gcaaactgta ccaactctgg cgttgtttgc gcttcctgca ataacaccag
    12181 atgtgcgtct ttgccgtaat tctttaatac cgacaaccat tcagcgcgtt gctgtttgta
    12241 tatgttccac accaggatcc gaatacgctc ttcggtactt aacggttctc cgggtggtaa
    12301 tgcctggccg atgctcgcaa aagaccccgg cggtaagatc ctctccgcag gttgtccggc
    12361 aacatagcgc atggcatagg tgttttttcg cacttttgac tttgaaacct ctattacgtc
    12421 aacccttccg cactcgggag ggttctactg ttatagggat tttagctcac tctttcaacg
    12481 agcaattgta tattgttatg taagcaagtg cttacaccgg tcagaaattt gctcattatt
    12541 gagaacatta aatcggatga catatcacgc atcctgatgg tttaatgaca aataaattca
    12601 aaatatctca tctattttgc aacaggagca aaaatgaaag ccacgtcgga agaactcgcc
    12661 atttttgttt cggtcgtcga aagcggcagc tttagccggg cagcggaaca attagggcaa
    12721 gcaaactcag cggtaagccg ggcggtgaaa aagctggaaa tgaaacttgg cgttagcctg
    12781 cttaatcgga ccacgcggca acttagcctg acggaagaag gcgagcgtta ttttcgtcgc
    12841 gtacagtcaa ttttgcagga gatggcagcg gcagaatcag aaattatgga gacgcgtaat
    12901 acaccgcgtg gactgttacg gatcgatgcc gcaactccag tggtgctgca ctttctgatg
    12961 ccgttaatta agcctttccg tgaacgctat ccggaagtca ctttatcgct agtctcctcc
    13021 gaaacgatta ttaatttgat cgaaagaaaa gtggatgtcg cgatacgcgc tggtacgtta
    13081 acggactcca gcttacgtgc caggccgtta tttaacagtt atcgaaaaat tatcgcctcc
    13141 cccgattata tttcccgcta cgggaagcca gaaacgatcg acgatttaaa gcaacatgtt
    13201 tgcctgggat tcactgaacc cgcttccctc aatacctggc cgatagcctg tagtgatgga
    13261 caattacatg aggtgaagta cggtttgtca tccaatagtg gggaaacact gaaacagctt
    13321 tgcctgagtg gcaacgggat tgcgtgtttg tccgactaca tgatcgacaa agaaatcgct
    13381 cgcggagaat tggtggagtt aatggcagat aaagtgttgc cagtggaaat gccattcagt
    13441 gccgtctatt acagcgaccg tgcggtaagt acgcgcatcc gggcttttat cgatttcctt
    13501 agcgagcatg taaaaacagc tcccggagga gctgtcagag aggcttaatc ccattcagga
    13561 gccagacctt ccgggctaac caggcggtcg ttgcaatcca gtgcggcgat cgctttttta
    13621 tcttcggcat caagctgtaa attttgtgcc ttaagattac tttccaggtt tttacgttta
    13681 gtagaagaag gaattactga gtaaccttcc cccatagccc acgccagaat cacttgtgcc
    13741 ggagtcgcat tgtgtttagc tgcaatacga gcaataacct catctttcag tgccttacca
    13801 taagccagcg tcatatagga agtgatatga ataccgtgct gtttagccca ggcaaccact
    13861 ttacggtttt gcagataagg agagagttca atctggttgg tagcgatgtt ttcagcgcca
    13921 acagcagcaa tcgctttttc catcaacggg atcgtgaagt tggaaatacc gatctcacgt
    13981 gtcagacctt gttttttggc ctccagcagc gcctgcataa actcttcaac agagacttca
    14041 tcgtttggtg acggccagtg gattagcgtc agatcaacat aatcggtacg caatttttgc
    14101 aggctctctt ttagactcgg gatcaatttg tctttgctga gattttcaat ccagatttta
    14161 gtggtgatgt agagttcatg acgtggcacg ccactttctg caatcgcctg acctactgcg
    14221 gcttcgttat catagatttg tgcagtatca attgcgcgat aaccaagttc aagcgccgtt
    14281 ttcacagatg aaataacaac gtcgtctttg agacggaaag tacctaaacc aaatgcaggg
    14341 atagccataa tatgcctctt ttaatgctat tttcgttaac tgaggtgatt atgggcttca
    14401 gactgttgcg gaaaaagagc ataaaatgca gaggattttt gcgattctgg caataataga
    14461 tatacagacg aaaaaaaacc tcgagcgtta actcgaggct tcttaataag tggcggaacg
    14521 gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta
    14581 ccgctccacc gaattctttt acaaccaccg gttttatgac cggcttactg cttaatttga
    14641 tgcctggcag tttatgaatc acttcgggct gttgcttcgc aacgttcaaa agctttcgct
    14701 tttgtcctac tctcttatcg agtagtgaac tgcctcaaag gtcagtgcca ttcttc
//
LOCUS       DAJTVK010000101        14404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000101 DAJTVK010000000
VERSION     DAJTVK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.101"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO372_003111"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO372_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6342533.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO372_003112"
     CDS             1828..3147
                     /locus_tag="OO372_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6342534.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="OO372_003113"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="OO372_003113"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6342535.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="OO372_003114"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="OO372_003114"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR6342536.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="OO372_003115"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="OO372_003115"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR6342537.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="OO372_003116"
     CDS             8179..9729
                     /locus_tag="OO372_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6342538.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="OO372_003117"
     CDS             9731..10753
                     /locus_tag="OO372_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6342539.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /locus_tag="OO372_003118"
     CDS             10750..11646
                     /locus_tag="OO372_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6342540.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="OO372_003119"
     CDS             11643..12629
                     /locus_tag="OO372_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6342541.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13548
                     /locus_tag="OO372_003120"
     CDS             12658..13548
                     /locus_tag="OO372_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6342542.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO372_003121"
     CDS             complement(13604..14035)
                     /gene="osmC"
                     /locus_tag="OO372_003121"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6342543.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3577 a         3557 c         3630 g         3640 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ctaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcacacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgacg ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagcgggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccagagcac tctcttcgca gcctgacgtg attgtgcttg atgagccaac ctcggcgctg
    13141 gatatctccg tgcaggcgca gatcctcaat ttactggtaa cgctacagga aaatcacggg
    13201 ctgacttatg tgctgatttc acacaatgtc tcggtgatac gtcatatgag cgatcgggtg
    13261 gcggtgatgt atctcgggca gattgtagaa ctgggcgacg cgcagcaggt gctgacggca
    13321 cctgcacatc catacacccg attattgctg gattccctcc ccgccattga taaaccgctg
    13381 gaggaagaat gggcattatg taaaacggat ctgccgggaa accgcacgtt gccacaaggc
    13441 tgttttttct acgaacgttg ccctctggca acccacggat gtgaagtccg gcaatcatta
    13501 gcaataaggg aggacggacg tgagctccgg tgctggcggg cgctgtagag atgctaacaa
    13561 cgcatcaggc attcacgcgc ctgacgcgtc atccggcaat gctttacgat ttcaactggt
    13621 aatccagcgt aatttccgct ttcagtacct gagaaaccgg gcatcctgct tttgctttct
    13681 ggatgatgcc gtcaaaggta gaggcatcaa tacccggcac cgcaacttca ctcttcagtg
    13741 cgattttcgt aatcgcaaaa tcggcatcca ctttatccag cgacacatcg gcggtggtat
    13801 caatcgatgt tggcgtgaat cccgcttccc ccagcattaa tgaaagcgcc attgagaaac
    13861 atgcggcatg cgctgcgcca atcagttctt cagggttggt tcctttttcg ccttcaaaac
    13921 gcgtgttaaa tccatacggc tgttggttca gcacgccact ctgggtggat actgttccct
    13981 tcccgcgttt gatatcgcct tcccagtgtg cctgaccttt cttatggatt gtcattgttg
    14041 ctctcctgtg ggcttaaaaa ggaaattata gactacgtga gaaacagcac gaggataagc
    14101 aggatattcc gaataaaata cggcgaaaat attacttctg cacttaaatt aaattcccac
    14161 aatagataac cgggaatata tatctcaaga gatattgcat caatatcaat caactcacca
    14221 caataataat acatatcaat atcatggagt taaaatataa aaatcatcaa ccaggactaa
    14281 tcttaacaac gaagcggcaa atatttgcca tgctgaatgt gctttataaa tctgcgatcc
    14341 gtagcagaca ccataaatac acagacacgg agaatcacta tgtttactta ttatcaggca
    14401 gaaa
//
LOCUS       DAJTVK010000102        14369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000102 DAJTVK010000000
VERSION     DAJTVK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14369
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.102"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..696)
                     /locus_tag="OO372_003122"
     CDS             complement(<1..696)
                     /locus_tag="OO372_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6342544.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="OO372_003123"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="OO372_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6342545.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="OO372_003124"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="OO372_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6342546.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="OO372_003125"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="OO372_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6342547.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="OO372_003126"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="OO372_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6342548.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="OO372_003127"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="OO372_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6342549.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="OO372_003128"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="OO372_003128"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6342550.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="OO372_003129"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="OO372_003129"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6342551.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="OO372_003130"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="OO372_003130"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6342552.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="OO372_003131"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="OO372_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6342553.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="OO372_003132"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="OO372_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6342554.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="OO372_003133"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="OO372_003133"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6342555.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="OO372_003134"
     CDS             10119..10499
                     /locus_tag="OO372_003134"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6342556.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="OO372_003135"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="OO372_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6342557.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OO372_003136"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="OO372_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6342558.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="OO372_003137"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="OO372_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="OO372_003138"
     CDS             complement(12845..14032)
                     /locus_tag="OO372_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6342559.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14369)
                     /locus_tag="OO372_003139"
     CDS             complement(14032..>14369)
                     /locus_tag="OO372_003139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342560.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3621 a         3606 c         3708 g         3434 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggtttac
//
LOCUS       DAJTVK010000103        14306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000103 DAJTVK010000000
VERSION     DAJTVK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.103"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="OO372_003140"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="OO372_003140"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6342561.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="OO372_003141"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="OO372_003141"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6342562.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO372_003142"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="OO372_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR6342563.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="OO372_003143"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="OO372_003143"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6342564.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     HVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="OO372_003144"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="OO372_003144"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6342565.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO372_003145"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="OO372_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6342566.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO372_003146"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="OO372_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6342567.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO372_003147"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="OO372_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6342568.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="OO372_003148"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="OO372_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6342569.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="OO372_003149"
     CDS             complement(13987..>14306)
                     /locus_tag="OO372_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6342570.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3443 a         3568 c         3995 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcacgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAJTVK010000104        14151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000104 DAJTVK010000000
VERSION     DAJTVK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.104"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..228)
                     /locus_tag="OO372_003150"
     CDS             complement(<1..228)
                     /locus_tag="OO372_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HCR6342571.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="OO372_003151"
     CDS             385..561
                     /locus_tag="OO372_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCR6342572.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
     gene            1187..1486
                     /locus_tag="OO372_003152"
     CDS             1187..1486
                     /locus_tag="OO372_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342573.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO372_003153"
     CDS             complement(1533..2420)
                     /gene="rarD"
                     /locus_tag="OO372_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6342574.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(2472..2939)
                     /locus_tag="OO372_003154"
     CDS             complement(2472..2939)
                     /locus_tag="OO372_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6342575.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3104..3973
                     /gene="pldA"
                     /locus_tag="OO372_003155"
     CDS             3104..3973
                     /gene="pldA"
                     /locus_tag="OO372_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6342576.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            4106..5935
                     /gene="recQ"
                     /locus_tag="OO372_003156"
     CDS             4106..5935
                     /gene="recQ"
                     /locus_tag="OO372_003156"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6342577.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            5999..6616
                     /gene="rhtC"
                     /locus_tag="OO372_003157"
     CDS             5999..6616
                     /gene="rhtC"
                     /locus_tag="OO372_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6342578.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO372_003158"
     CDS             complement(6678..7298)
                     /gene="rhtB"
                     /locus_tag="OO372_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6342579.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            7409..8431
                     /gene="pldB"
                     /locus_tag="OO372_003159"
     CDS             7409..8431
                     /gene="pldB"
                     /locus_tag="OO372_003159"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6342580.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8439..9239
                     /gene="yigL"
                     /locus_tag="OO372_003160"
     CDS             8439..9239
                     /gene="yigL"
                     /locus_tag="OO372_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6342581.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            9315..10206
                     /gene="bioP"
                     /locus_tag="OO372_003161"
                     /pseudo
     CDS             9315..10206
                     /gene="bioP"
                     /locus_tag="OO372_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
     gene            complement(10094..11047)
                     /gene="metR"
                     /locus_tag="OO372_003162"
     CDS             complement(10094..11047)
                     /gene="metR"
                     /locus_tag="OO372_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6342582.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            11283..13544
                     /gene="metE"
                     /locus_tag="OO372_003163"
     CDS             11283..13544
                     /gene="metE"
                     /locus_tag="OO372_003163"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6342583.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(13829..14074)
                     /locus_tag="OO372_003164"
     CDS             complement(13829..14074)
                     /locus_tag="OO372_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6342584.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3444 a         3421 c         3689 g         3597 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 attatgtggt ttgcatatat atatgcaata tagcagtctt tgcattgata actatacagt
     1081 agtaatttag agtgtgtaag tttttattga tggggtatat tttaattaaa tataattgaa
     1141 atgtattttt attctataaa caatatcttg ggtgaatgga ttctatatgg gaaatggtaa
     1201 cttttggcag ttgttcttgg gtaaagaacc ttacagcaaa aaatctgatt ttgttaaggc
     1261 atggttattg tggtgggcta ttaccatcct tgttctattt attttaagcc tgactacgat
     1321 attaaattta agaagtatag gtatagggtt aagttttggg ttttgtcaaa tccttggtag
     1381 atatacgatg tctctaggat ggagtaagta ctggggagtt ttgggggtat ttcctgtaac
     1441 aaatattgtt ctttttctta tcttaatttt tttcaaaaaa agataaataa gtcagtgggt
     1501 aggctcttta aacggcctac ccatgttaat tcttaacttt ttctacgctg cgtataaatc
     1561 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagtcac catcttatcg
     1621 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cgggccaatg
     1681 tactggaaaa agcctaacgt tgagagacgc aagcgcgtgg cagcggcggt aaaacacaac
     1741 agcggtacgg tagtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
     1801 tgccccatat ggctggttga gctgtcggca atagcaaaca ggtaaattgc cgccacgggc
     1861 agcagccaca tggtttcgat taacatgccg gtttgcgctt caacggcaat cttcttgcgt
     1921 accagaccgt agaaggcaaa actaaatgcc agtcccagcg cgataatagg tagcgaacca
     1981 aaagtccaca gctggactaa cacgccacat atcgccagaa tcaccgccag ccattgcatc
     2041 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
     2101 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
     2161 cagttgccac caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     2221 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     2281 aacgaccaga tcacgcgatg cgtcaggatt tcatcggcgg gcacgtagta aatcaacttg
     2341 aagtacgctg gcgctatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     2401 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     2461 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     2521 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgcaa caggctactg
     2581 gtagcagtaa aacgctcgcc cctgcctggg cgcagataat caacgcgaag atcaatggtc
     2641 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     2701 gtacttccca cgcacaccag accggcggcg acatccagcg ccgacgcaat gaccccgccg
     2761 tgcaaaatgc tttgcgccca gttgcccacc atcattggct gatttttaaa ggccagctgt
     2821 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     2881 taaacaaaca tctcacccac taatttcagg gcttgttcag cggtcagtac ggcagacata
     2941 tcatccttac acttcattgg ttaatgaaat gttgatttta tgcttctttg ttgttggttt
     3001 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     3061 aaatattcac cttatcaata attcgttacg gagaacacga ccgatgcgga ctctgcaggg
     3121 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     3181 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     3241 tgacaatccg ttcacgctct atccttatga caccaactac ctcatttaca cccaaaccag
     3301 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaatgcgc gtaaggatga
     3361 agtaaagttt cagttgagcc tggcatttcc gctgtggcgt gggattttag gcccgaactc
     3421 ggtgttgggt gcgtcttata cgcaaaaatc ctggtggcaa ctgtccaata gcgaagagtc
     3481 ttcaccgttt cgtgaaacca actacgaacc gcaattgttc ctcggttttg ccaccgatta
     3541 ctgttttgca ggttggacgc tgcgcgatgt ggagatgggg tataaccacg actctaacgg
     3601 gcgttccgac ccgacctccc gcagctggaa ccgcctttat actcgcctga tggcagaaaa
     3661 cggtaactgg ctggtagaag tgaagccgtg gtatgtggtg ggtaatactg acgataaccc
     3721 ggatatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     3781 ggtgttgagt gcgaaaggac agtacaactg gaataccggt tacggcggcg cggagttagg
     3841 cttaagttac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggctatgg
     3901 cgaatcgctc atcgactata acttcaacca gacccgtgtc ggtgtaggag ttatgctaaa
     3961 cgacttgttt tgatgaacgg ttgagtggtt ggcaaatctg gaacccagca tccaggatta
     4021 ccctctcaga gactaaaagc attgcagttt ctcgcgcagg cgctgaaaat agcgcctgtt
     4081 tttatttcag gcaatcgggg ggaatgtggc gcaggcggaa gtgttgaatc tggagtccgg
     4141 agctaaacag gttttacaag aaacctttgg ctaccaacag tttcgccccg gccaggaaga
     4201 aattatcgac actgtgcttt ccggtcgcga ttgcctggtc gtcatgccca ccggtggcgg
     4261 aaaatccctt tgctatcaaa ttcctgcctt attgctaaac ggccttaccg tggttgtttc
     4321 accgctgatt tcgttgatga aagatcaggt ggatcaactg caagccaacg gcgtggcggc
     4381 ggcgtgcctt aactcgacgc aaacccgcga gcagcaactt gaagtgatga caggctgccg
     4441 caccgggcaa attcgtctgc tttatatcgc cccggaacgc ctgatgctgg ataactttct
     4501 tgagcatctg gcgcactgga atccggtgtt attagccgtt gatgaagcgc actgtatctc
     4561 ccaatggggc cacgatttcc gcccggaata tgccgcgctt ggtcagttac gccagcgttt
     4621 cccgaccctg ccgtttatgg cgctgactgc cacagccgac gacaccacgc gccaggatat
     4681 cgtgcgcttg ctggggctga acgatccgct gattcaaatc agcagttttg accgtccgaa
     4741 tattcgctac atgctgatgg agaaattcaa accgctcgat cagttgatgc gctacgtgca
     4801 ggaacagcgc ggtaagtccg gcattatcta ctgcaacagc cgggcgaaag tagaagacac
     4861 cgctgcgcgc ctgcaaagca agggtattag cgcggcggcc tatcatgccg ggctggaaaa
     4921 taatgtccgc gccgacgtgc aggagaaatt ccagcgcgat gacctgcaaa ttgtggtggc
     4981 gacggtggcg ttcggcatgg gcatcaataa accaaacgtt cgcttcgtgg tccactttga
     5041 tattccgcgc aatatcgaat cctattatca ggaaaccggt cgcgccgggc gtgatggcct
     5101 gcctgcggaa gcgatgctgt tttacgatcc ggctgatatg gcgtggctgc gccgttgtct
     5161 ggaagagaag ccgcaggggc agttgcagga tattgagcgc cacaaactca atgcgatggg
     5221 cgcgtttgcc gaagcgcaaa cttgccgtcg tctggtattg ctgaactatt ttggcgaagg
     5281 gcgtcaggag ccgtgcggga actgcgatat ctgcctcgat ccgccgaaac agtacgacgg
     5341 ttcaaccgat gctcagattg ccctttccac cattggtcgt gtgaatcagc ggtttgggat
     5401 gggttatgtg gtggaagtga ttcgtggtgc taataaccag cgtatccgcg actatggtca
     5461 tgacaaactg aaagtctatg gcatgggccg tgataaaagc catgaacatt gggtgagcgt
     5521 gatccgccag ctgattcacc tcggcctggt gacgcaaaat attgcccagc attctgccct
     5581 acaactgaca gaggccgcgc gcccggtgct gcgcggcgaa tcctctttgc aacttgccgt
     5641 gccgcgtatc gtggcgctca aaccgaaagc gatgcagaaa tcgttcggcg gcaactatga
     5701 tcgtaaactg ttcgccaaat tacgcaaact gcgtaaatcg attgccgatg aaagtaatgt
     5761 cccgccgtac gtggtgttta acgacgcaac cttgattgag atggctgaac agatgccgat
     5821 caccgccagc gaaatgctca gcgttaacgg cgttgggatg cgcaagctgg aacgctttgg
     5881 taaaccgttt atggcgctga ttcgtgcgca tgtcgatggc gacgacgaag agtagtcagc
     5941 agcataaaaa agtgccagga tgaagactcc gtaaacgttt cccccgcgag tcaaatgtat
     6001 gttgatgtta tttctcaccg tcgccatggt gcacattgtg gcgcttatga gccccggtcc
     6061 cgatttcttt tttgtctctc agaccgctgt cagtcgttcc cgtaaagaag cgatgatggg
     6121 cgtgctgggc attacctgcg gtgtaatggt ttgggctggg attgcgttgc ttggcctgca
     6181 tttgattatc gaaaaaatgg cctggctgca tacgcttatg gttggcggtg gcctgtatct
     6241 ctgctggatg ggttaccaga tgctgcgcgg cgcgctgaaa aaagaggcgg tttctgcacc
     6301 tgcgccacag gtcgagctgg cgaaaagtgg acgcagtttc ctgaaaggtt tactgaccaa
     6361 tctcgctaat ccgaaagcga ttatctactt tggctcggtg ttctcattgt ttgtcggtga
     6421 taacgttggc actaccgcgc gctggggcat ttttgcgctg atcattgtcg aaacgctggc
     6481 gtggtttatc gtcgttgcca gcctgtttgc cctgccgcaa atgcgccgtg gttatcaacg
     6541 tctggcgaag tggattgatg gttttgccgg ggcgttattt gccggatttg gcattcattt
     6601 gattatttcg cggtgatgcc agacgcgtct tcagagtaag tcggataagg cgtttacgcc
     6661 gcatccgaca ttatttttca cgcatgcctc gccgatgcta acagcgctcc caccagcata
     6721 aacaacgagc cgaaaatctt attcagcgcc ttcatctgct ttggtccttt aatccataga
     6781 gcaatccgtt gagcaagggt ggcgtaaccg atcatcacaa taatatcgac cacaatagtg
     6841 gtgacgccga gcacgatata ctgcatcagt tgcggctgtt gcggcatgat gaattgcgga
     6901 aatagcgccg ccagaaacac aatacttttg ggattggtga gattcacaaa aactgcgcgc
     6961 tggaacaaat gtcgacgcga ttgagtagag gccagcgatt taaggtcaat tgcaccagcg
     7021 gcgcgccact gctggattcc cagccaaatc aagtaagccg cgcctgccca cttcaacact
     7081 tcaaacgcaa tcactgagcg ggaaaatagc gtccccaacc ccacgccaac cagcacaata
     7141 tgaatcgcca gtccggtctg aagcccagca atagacgcca ccgcgccgcg ataaccgtgg
     7201 ttgagcgagg tggtcatagt gttgattgca ccagagcctg gcgacaggct taaaatgatc
     7261 gatgtcagca ggtaggcaaa ccaccattct aaggtcatga tgaactcccg gtgtgtctat
     7321 ttttgtgcca caatacgcta ctgtcgcagc gttgtgtcag gcacgctaaa aaaaacgatt
     7381 ttacgtggtt taagaggcag attacccgat gtttcagcag caaaaagact gggaaacaag
     7441 agaaaacgcg tttgctgctt ttaccatggg accgctgact gatttctggc gtcagcgtga
     7501 tgaagcagag tttactggtg tggatgacat tccggtgcgc tttgtccgtt ttcgcgcaca
     7561 gcaccatgac cgggtggtag tcatctgccc ggggcgtatt gagagctacg taaaatatgc
     7621 ggaactggcc tatgacctgt tccatttggg gtttgatgtc ttaatcatcg accatcgcgg
     7681 gcagggacgt tccggtcgcc tgttagccga tccgcatctc gggcatgtta atcgctttaa
     7741 tgattatgtt gatgatctgg cggcattctg gcagcaggag gttcagcccg gtccgtggcg
     7801 taaacgctat atactggcac attcgatggg cggtgcgatc tccacattat ttctgcaacg
     7861 ccatccaggt gtatgtgacg ccattgcgct aactgcgcca atgtttggga tcgtgattcg
     7921 tatgccgtca tttatggcac ggcagatcct caactgggcc gaagcgcatc cacgtttccg
     7981 tgatggctat gcaataggca ccgggcgctg gcgcgcgttg ccgtttgcta tcaacgtact
     8041 gacccacagc agacagcgat atcgacgtaa cttacgcttc tatgctgatg acccaacgat
     8101 tcgcgtcggt gggccgacct accattgggt acgcgaaagt attctggctg gcgaacaggt
     8161 gttagccggt gcgggtgatg acgccacgcc aacgcttctc ttgcaggctg aagaggaacg
     8221 cgtggtggat aaccgcatgc atgaccgttt ttgtgaactc cgcaccgccg cgggccatcc
     8281 tgtcgaagga ggacgaccgt tggtaattaa aggtgcttac catgagatcc tttttgaaaa
     8341 ggacgcaatg cgctcagtcg cgctccacgc cattgttgat tttttcaaca ggcataactc
     8401 acccagcgga aaccgctcta cagaggttta aatttcttat gtaccaggtt gttgcgtctg
     8461 atttagatgg cacgttactt tctcccgacc atacgttatc cccttacgcc aaagaaaccc
     8521 tgaagctgct caccgcgcgc ggcatcaact ttgtgtttgc gaccggtcgt caccacgttg
     8581 atgtggggca aattcgcgat aatctggaga tcaagtcgta catgatcacc tccaacggcg
     8641 cgcgcgttca cgatctggat ggcaatctga tttttgctca taacctggat cgcgacattg
     8701 ccagcgatct gtttggcgta gtcaacgaca atctggacat cattactaac gtttatcgcg
     8761 acgacgaatg gtttatgaat cgccatcgcc cggaagagat gcgctttttt aaagaagcgg
     8821 tgttcaaata tgcgctgtat gagcctggat tactggagcc ggaaggcgtc agcaaagtgt
     8881 tcttcacctg cgattcccat gaacaactgc tgccgctgga gcaggcgatt aacgctcgtt
     8941 ggggcgatcg cgtcaacgtt agtttctcta ccttaacctg tctggaagtg atggcgggcg
     9001 gcgtttcaaa aggccatgcg ctggaagcgg tggcgaagaa actgggctac agcctgaagg
     9061 attgtattgc gtttggtgac gggatgaacg atgccgaaat gctgtcgatg gcggggaaag
     9121 gctgcattat gggcagtgcg caccagcgtc tgaaagacct tcatcccgag ctggaagtga
     9181 ttggtactaa tgccgacgac gcggtgccgc attatctgcg taaactctat ttatcgtaat
     9241 cgttctttat ttggtcagtt gtcaacctga tacttcgcta caatggatac tcgttaatca
     9301 aagagttttc cattgtggcg ctacttatca tcaccacgat tctgtgggcc ttctccttta
     9361 gcttttatgg cgagtacctt gcggggcacg tcgatagcta ttttgcggtg ctggtgcgcg
     9421 ttggcctggc ggcactcgtt tttctgccgt ttctgcgtac ccgtggcaat agcctgaaaa
     9481 cggtcggcct gtatatgctg gtgggcgcga tgcagctcgg cgtgatgtat atgctgagtt
     9541 tccgcgctta tctctacctg acggtctccg aattgttgct gttcaccgta ctgacgccgc
     9601 tcgctgattt atgacatcat gagtaagcgc cgtctgcgct ggggctatgc ttttagcgcc
     9661 ttgctggcgg tgattggtgc cgggattatt cgctatgatc aggttaccga ccatttctgg
     9721 actggcttgt tgctggtgca actctccaat atcacttttg ccatcggcat ggtgggttac
     9781 aaacgcctga tggaaactcg cccgatgccg cagcataacg cctttgcgtg gttctatctt
     9841 ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca gaaaatgccg
     9901 caaaccacgt tgcaatgggg cattctggtg tttcttggcg tggtggcttc cgggattggc
     9961 tactttatgt ggaactacgg cgcgacgcag gtggacgccg gaacgctggg cattatgaat
    10021 aatatgcacg ttccggcagg gctgctggta aacctggcta tctggcacca acagccgcac
    10081 tggccaacgt tcattacagg cgcgctggtg atcctggcct cactgtgggt gcatcgtaag
    10141 tgggtcgctc cgcgctcttc acaaacggca gatgatcgca ggcgtgattg cgcgctgagc
    10201 gaataaatgc ttctgtaatc ggctgacgct gctcgccatc gcgcacggcg gcgtacagtc
    10261 ggctccacaa gccttcgccc agggttttgg tcaccaccag accttggcgc tcaaaactct
    10321 ctactaccca atgcggtagc gcggcaatac ccatccgcgc ggcaaccatc tgaatcaaca
    10381 ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc tgccggctga agaaaatgcc
    10441 gccagacatc cagtcgacta cgctgcaccg gataaatcaa cagcgtctcg ctggcgagat
    10501 cttccggtgt aattcgcgtt ttcgccgcca gtggatggtc aggtgctaac accagacgca
    10561 cttcatagtc gaacatcggc gaataatgca ggccactgcg cggcaggata tcggaggtca
    10621 tcaccagatc cagctcgccc tgttgcaacg caggctgcgg gtcaaacgtc acgcccgatt
    10681 taaaatccat ctctacctgc ggccagttct tatggaaatt ttctaacgcg ggcgtcagcc
    10741 actgaataca gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc ggttcattgc
    10801 aggcttgcag tgcctggctg atttgcggca gcacctggtt tgccagttgc aacaggattt
    10861 ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac aaatagccgg aagccaaggc
    10921 gttgttccag atcgctaaac tggtgagaca gggcggattg cgtctgatgc aacgtcgccg
    10981 cagcggctgc gagagagccg cagttccgca acgcttgtag cgttttcagg tgttttactt
    11041 cgatcatgaa agtccttcac ttcggcatga ataatttgcg cttgaggaat atacagtaac
    11101 cgccaattat ggatgtgtaa acatctggac ggctaaaatc cttcgtcttt taaatttatg
    11161 gtgcgttggc tgcgtttctc caccccggtc acttacttca gtaagctccc ggggatgaat
    11221 aaacttgccg ccttccctaa attcaaaatc cataggattt acatataatt agaggaagaa
    11281 aaatgacaat attgaatcac accctcggtt tccctcgcgt tggcctgcgt cgcgagctga
    11341 aaaaagcgca agaaagttat tgggcgggga actccacgcg tgaagaactg ctgacagtag
    11401 ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca agcgggtatc gacctgctgc
    11461 cggtgggcga ttttgcctgg tacgatcatg tactgaccac cagtttgctg ctgggtaatg
    11521 ttccgccacg tcatcagaac aaagacggtt cggtagatat cgacaccctg ttccgtattg
    11581 gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc aaatggttta
    11641 acaccaacta tcactacatg gtgccggagt tcgttaaagg ccaacagttc aaactgacct
    11701 ggacgcagct gctggaggaa gtggacgagg cgctggcgct gggccacaac gttaagcctg
    11761 tgctgctggg gccggttact tacctgtggc tggggaaagt gaaaggtgaa cagtttgacc
    11821 gccttagcct gctgaacgac attctgccgg tttatcagca agtgctggca gaactggcga
    11881 aacgcggcat cgagtgggta cagattgatg aacccgcgct ggtactggaa ctaccacagg
    11941 cgtggctgga cgcatacaaa cccgcttacg acgcgctcca gggacaggtg aaactgctgc
    12001 tgaccaccta ttttgaaggc gtaacgccaa atctcgacac gattactgcg ctgcctgttc
    12061 agggtctgca tgttgatctc gtacatggta aagatgacgt tgctgaactg cacaagcgcc
    12121 tgccttctga ctggttgctg tctgcgggtc tgatcaatgg tcgtaacgtc tggcgcgccg
    12181 atcttaccga gaaatatgcg caaattaagg acattgtcgg caaacgtgat ttgtgggtgg
    12241 catcttcctg ctcgttgctg cacagcccca tcgacctgag cgtggaaacg cgtcttgatg
    12301 cagaagtgaa aagctggttt gccttcgccc tacaaaaatg ccatgaacta gcactgctgc
    12361 gcgatgcgct gaacagtggt gacacggcag ctctggcaga gtggagcgcc ccaattcagg
    12421 cgcgtcgtca ctctactcgt gtacataatc cggcggtaga aaagcgtctg gcggcgatca
    12481 ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc cagcgtgcac
    12541 gttttaaact gccagcgtgg ccgaccacca cgattggttc cttcccgcaa accacggaaa
    12601 ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga cgccaataac taccgcacgg
    12661 gcattgcgga acatatcagg caggccattg ttgaacagga acgtttggga ctggatgtgc
    12721 tggtacatgg cgaggccgag cgtaatgaca tggtggaata ctttggcgag cacctcgacg
    12781 ggtttgtctt tacgcaaaac ggttgggtac agagctacgg ttcccgctgc gtgaagccac
    12841 cgattgttat tggtgacgtt agtcgcccgg caccgattac cgtggagtgg gcgaagtatg
    12901 cgcaatctct gactgataaa ccggtgaaag ggatgctgac tggcccggtg actattctct
    12961 gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacag attgcgctgg
    13021 cgctgcgtga tgaagtggcc gatctggaag ccgctggaat tggcatcatc cagattgacg
    13081 aaccggctct gcgcgaaggt ttaccgctgc gtcgtagcga ctgggatgcg tatctccagt
    13141 ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa agatgacaca caaatccaca
    13201 ctcacatgtg ttattgcgag ttcaacgaca tcatggattc gattgcggca ctggacgcag
    13261 acgtcatcac catcgaaacc tcgcgttctg acatggagtt gctggagtcg ttcgaagagt
    13321 ttgattatcc gaatgaaatc ggtccaggcg tctatgacat tcactcgcca aacgtaccga
    13381 gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa acgcattccg gcagagcgcc
    13441 tgtgggttaa cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc cgcgcggcac
    13501 tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg atgatgaata ttaacgagtc
    13561 actgtaaatt gcagtacgat gaaagaggca aaactgcctg atactaggcc tacataattt
    13621 atgcaattta ttgaattttt gggttttgtt ggccggacaa ggcgttcacg ccgcatccgg
    13681 catgaacaac gcgcactttg ccaataatct gatggcttcc atcaccggaa gccattttgc
    13741 tatctgcgcc cgttcagata cagaaataac cgaatcattt ttacgcaaaa tataatcctg
    13801 agcgatttgt tcagtaatca acgctacgtt atttattctc acttttcacg aaattgtatc
    13861 ccttttcagt aacgtggtag tgaggcatag acttatgtcc gctatcctca ataataacca
    13921 aaccctcgtt agctaatttg ttaacaatgt taacaacatt accgaatcct ggaatttgcc
    13981 ttatggatag tgtgcgatcg agattcatcc agttccagtt tatatctttc tcagccataa
    14041 ttttcagtac gttaatttca gtttcagtta tcatagtcaa gtgcatcctg gtggtataag
    14101 tcattaagtt ctcccttaag tttataatct tctagagtag cagtatgcgt a
//
LOCUS       DAJTVK010000105        14121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000105 DAJTVK010000000
VERSION     DAJTVK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.105"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(112..700)
                     /locus_tag="OO372_003165"
                     /pseudo
     CDS             complement(112..700)
                     /locus_tag="OO372_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(857..3037)
                     /locus_tag="OO372_003166"
     CDS             complement(857..3037)
                     /locus_tag="OO372_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6342585.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(3136..3342)
                     /locus_tag="OO372_003167"
     CDS             complement(3136..3342)
                     /locus_tag="OO372_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342586.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            complement(3367..4077)
                     /locus_tag="OO372_003168"
     CDS             complement(3367..4077)
                     /locus_tag="OO372_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6342587.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            complement(4553..4858)
                     /locus_tag="OO372_003169"
     CDS             complement(4553..4858)
                     /locus_tag="OO372_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342588.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            complement(5005..5220)
                     /locus_tag="OO372_003170"
     CDS             complement(5005..5220)
                     /locus_tag="OO372_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342589.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            complement(5441..5731)
                     /locus_tag="OO372_003171"
     CDS             complement(5441..5731)
                     /locus_tag="OO372_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342590.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            complement(6055..6351)
                     /locus_tag="OO372_003172"
     CDS             complement(6055..6351)
                     /locus_tag="OO372_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342591.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            complement(6351..7304)
                     /locus_tag="OO372_003173"
     CDS             complement(6351..7304)
                     /locus_tag="OO372_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6342592.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            complement(7687..8349)
                     /locus_tag="OO372_003174"
     CDS             complement(7687..8349)
                     /locus_tag="OO372_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6342593.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
     gene            8865..9434
                     /locus_tag="OO372_003175"
     CDS             8865..9434
                     /locus_tag="OO372_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6342594.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            10162..10335
                     /locus_tag="OO372_003176"
     CDS             10162..10335
                     /locus_tag="OO372_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6342595.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(10593..11921)
                     /gene="rutG"
                     /locus_tag="OO372_003177"
     CDS             complement(10593..11921)
                     /gene="rutG"
                     /locus_tag="OO372_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6342596.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(11942..12436)
                     /gene="rutF"
                     /locus_tag="OO372_003178"
     CDS             complement(11942..12436)
                     /gene="rutF"
                     /locus_tag="OO372_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6342597.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(12447..13037)
                     /locus_tag="OO372_003179"
     CDS             complement(12447..13037)
                     /locus_tag="OO372_003179"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6342598.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(13047..13847)
                     /gene="rutD"
                     /locus_tag="OO372_003180"
     CDS             complement(13047..13847)
                     /gene="rutD"
                     /locus_tag="OO372_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6342599.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(13855..>14121)
                     /gene="rutC"
                     /locus_tag="OO372_003181"
     CDS             complement(13855..>14121)
                     /gene="rutC"
                     /locus_tag="OO372_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6342600.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         3869 a         3274 c         2917 g         4061 t
ORIGIN      
        1 tttgcacact gagtactttt taagctttga agctactcga ccagcgttac tattagcatt
       61 ggtgacgttg gttttttcag cttctgttaa gtctgaatag tccagcttcc aagccttatt
      121 acatttaatc tgagcatcga gaatatcatt tctgatagct ggagcgtaac cggatacatc
      181 gctaatattc attaactctt tgcacgatag cataggcgcg ttattgcgtt cagtggtcac
      241 gttacctgca ttcttcttac tggtcacacc accgtcattt gtggtagccg ttttgccagt
      301 ggctgctgaa atatcagttt gacctacgat tgctttaaag ttaatttcct gaaaagttag
      361 ctggatacga agaccgttag agatagtaac atctttagag gctttaaagt tggtaataat
      421 ggaatcgtca attttaattc catctttaca gataacagaa ataatctgct tctggtcacg
      481 ccagttttcc agagtgtcaa tgaagttttc tactaattga ccctgacgag ttaataataa
      541 gcttccttca taattgcatg ttctgtgtag tcacctgcat agggctatca aattccatgt
      601 tttcagttgc tgataaggtt aagaaggcat ctacattgtc tcttaagtgg aaatagatgc
      661 catctttgcc actgtaacga agctgattag tgtaaatcat taaattctcc tttgatttca
      721 aaggcttagt gtattttaat catttttaac caagcctttg atatatatgt attttctctg
      781 ttaagcgtga gctacgtgaa aaataatttt tggtgtgggt attaactttg ctttattcac
      841 gtagaacacc gttaaatcat tcttcctctg gatagcttga acttaagagc atatcctgcg
      901 tgaactggtc attagctgat tgattgaaag taaagaaaga tggttcatct ggtcgaacga
      961 caacttgacc accaagtgaa actttcactg gctcctgaat acgcttactc aaagcactaa
     1021 tttcatccct cagaccctga atatctgcac tcgttaatgc tgaatcttgc ttaatgttgt
     1081 ttaactctgg atgattgaat cttgcaatat tcattgtggc attgttaaac aattcaccca
     1141 ttgaactaaa aattctactc catccttcgt taatcatatt cggtttatta gggtcaccag
     1201 aatacacggg attaaagtca ctacccacac ctaccccaga cggatgcata ttagcgtttt
     1261 tgactacttc ctgattcata aaatccagtg ctttcttcaa tgcatataaa ccaagagtga
     1321 cgcctctgat ttgtggaatc attgaagcta atgctaacgc tgtttcataa ccgtcatcag
     1381 cataaccaat tgcaagagca ctacctacac caccagctac acctttcagt tttccaccac
     1441 ctttaccagt agctacctga ccagccacct gattagctct tgtacctaac gttgtgattg
     1501 ctcttgtcag gttcataacc cctgtagtag cactgaatag cgtctttgca gataacgcta
     1561 tagcaagacc tttgacgaag ttgataaaac catcaccgac ttcatcaagt agttttcgtt
     1621 tagtgtcatc aaggctgtaa tagaacttct tagtctgtaa aatccagcct tcaatggtgt
     1681 tattgaatac gcgaatatca tctacaccag ctttaagcat gtcgataagt ccaccgatag
     1741 catcaccagc gatagaagaa ctcatcccca tttcactaag caggttactc aggctggata
     1801 agaagccgtt tagctcatcc tgtgaacctg agaagatttt caccatcgta tcatcccaat
     1861 tgtttgctaa cttacctaaa gctgtctgag tgctcttagt gacttcagcc cagccacccg
     1921 ctttatcaat catcaagccc atctgtttag cgactttagg gagagtatca gcggaaagta
     1981 ccttcccgtt tttcatggct gcatcaagct ctgtagcagt catacccaat gcttcagcga
     2041 atagcttaaa tgcaccagcc atacggtcac ctaactgccc acgtaattcc tcagcctgaa
     2101 tcttgccctt gctcgccatc tgctcaaatg ctctcaaaat gccgtttagc tcatcgttag
     2161 aaagccccat taagcgactc atagccgttg cattaccaaa tagctcctgt gtctgttcct
     2221 gtgacataga accctgagca cctgctgcaa acttagcgta gcttgatagc ccttcagaga
     2281 tattaagacc tgtcttgtct gctaaatttc gaataaatac ctcagcttca gatgctttat
     2341 caccaagtac aaacttagcg gctctctgtg cggaatctcg tttaagacct gcattcataa
     2401 tggccttgta agcgttaaga gcacccatca cagagtaaaa tgcaccagat aatttaatcg
     2461 cattgcttgc aagattatta aatgcgtcgc ttgtattgtt aatgctggcg gtctttgtct
     2521 gtgacctgag tttattcaac gtttcacgaa gagattgatt taattttgca agttcagcgt
     2581 taaacttttc acttccaata ttaccagcct taccgcttaa gctgtaatac tgtgctttaa
     2641 acattttagc catcagcgga ttaatatcac caagcttaga catattcacg ctatgcgctg
     2701 ctgtgattgc tctgtttccg ctaattcgtg gttgccttga ttgttttgga ggattaacag
     2761 tgtcattaaa tctggtggca aactgaaatg cttttgtgct tcttgctctc tgctcaatct
     2821 tgtcaaaggt cttattaaac ttcctttcaa gctgccctaa agaacttgtc agattgctgt
     2881 caagttgctt ggtatcgacc ttaaacgctc tattcaaatt gcgttcaatt ctttgtgctg
     2941 actgcaaagc tgccttttca actctttgca agcccttaat aacgttttca ctgaaactaa
     3001 gttcaacgat aaaactatca actgtgaatt tgctcattca tgtttcacct ctttaagtgt
     3061 tcatatacga aaaagggaaa gactaccgat atcactcgat aatctctccc gtagattttc
     3121 acccaaccaa tcttgttacc taaccagata ataattacca tcttcaccaa caatctgaca
     3181 atcacttggt aatttatcaa gacaccactt agctattttc ctgattctat tcttttgctt
     3241 agaggaggtg aatgatttga ttcgttttgg tttgttgggt tgttctttca atcgttcact
     3301 aattgcttta ataagctcat agcgttctgt taaatcaatc attcaccacc accttttaat
     3361 tattaattaa atagcctttg catatttgct agaaatcttc gtaagctgct caatatcagc
     3421 ctgcatagca atcttcttag catcttcata ttctttcaca agctctttca tagcctgaag
     3481 ctctcttgag agatacaact taacattatc aagttgcttg ttacgtgatg ccaagatgtt
     3541 ctgtctagca atttcagcga gttcttcatc gctcttatta gtcatgtaaa taaactgacc
     3601 agcacctctt gatgccttaa ccatcttcac aattcgatgt ccagcaggaa gactactaat
     3661 cacggctgcg acgttttctt gaaaagtacc aaaaccatca ttatcttcgt attggtaata
     3721 gacaaatggt ttctgtgaca attcatcctg aatctcaaat tctgacggct caatagctga
     3781 ttgcatcaag gtaactttgc tgtaagcgtc atctaatcta aggcgctgtt ctttcagcag
     3841 ttggtaatat ttcttttcaa gctcaagtaa tgcttcactc ttgcaaatat caggcatctc
     3901 ttgaggctca ggagacacaa agatggttgt ttttggtgac atctttagcg attgctcaag
     3961 ctcaatacgc ttgtcatgct ctttacgctg aatagtgata gctggatgtt cagtctgtgg
     4021 cgaccagtca ctgaagctat caatcacttt gccattaatg taaatatcat ttgtcattgc
     4081 ttttcctctt ctaagcggaa gtgtttcagt ttttaaaaag cacacctgac agcctatcta
     4141 aacttagaag agacttaaaa ggcggtttgc caagtatgct ttttaaaagc tgaatagccc
     4201 tagataattt cttataaagg gccattcata aggtgaagaa attaaaacat ctaaaggatt
     4261 caaaaaggat tcaaacgaat gaacaagagt acttttcgag atgacctctc agactaacct
     4321 gaaaggtcat gtagcgaaaa gcattgcacc tttcagaata actatcattg atgtagaatg
     4381 attaatagtt taattcagga agagttgctc ttccctatta ccatcatctc atgcttaagg
     4441 ttatttgtca acagcttttt aacagattgg taacatcaat tcttatttac cgtattacct
     4501 acccagcaac caacaaataa ccctaaagtc atccagagaa taacgagtgt cattacatca
     4561 cctcgctgta agggtttaat tcattctgat aggtcaagaa tacattcagc ttcaataatt
     4621 gctcagggct taaatcaagt cgattgcact cttcaatgat aaagcgctta ttgtatgggt
     4681 ctgttacttc cagcttgtga acatagtcac caagtttaat ttcgttgatt atatgcttgt
     4741 caatatacgt tgacgcttca aaacaatgca tgaaatagat attaaagccc gacctgttca
     4801 ggtcaatcaa ccaattcatg gtttcttggt caaccataat ttgcattggg taattcattt
     4861 ttaaaatcct tcctgtatcg cattctaaga cgttttaacc ccttttttag accacttaag
     4921 gcgattccct aagttaccct gattaggggc ttaaaaacgt ttctggtgcg tctggtaaag
     4981 cctgagagat atgttttgct gtttttagct caagtctgag actaatagcc ccatctggtg
     5041 accgtagtct tttaaccagc tttgcttaac cggagtcttc agagaatagt taggggctga
     5101 caagtgaaga tagatatcat ctttgccgtg gatacttccc catccttcgt ataactcaag
     5161 caagttttca aaataacgaa atgctttatt agtctgaagt tgtactgtga tagttttcat
     5221 caagcacccc attcagaaat aagcttaatt ccctgctcaa gctgttcagg gtcgttatag
     5281 tcgagtatta ccagtggctt taattcgtgt ttaagctggt ggatttgaga gggttttaat
     5341 cgtttgtttg ctttgagggt cacatcttca aattcagcat ctgctaaatt ctgtctgatt
     5401 aggtggcggc caagtgtgga atattggaac ctataacaca ttaaacctcc aatctatccg
     5461 ccagttgatg gaccttagcg cttaacacct gaatcattga gcaacctgct actgcggact
     5521 gtgctttgag tagttccagt tggcccgttg tgattctgat ggccttagaa ccctctgcga
     5581 cgcccttaac gttatcgaaa gggctaaggt ttaccgactc ttttaaaatc tccccaacaa
     5641 gcgctgtgtg cttcatatcg ggatatttgg ctttgattgc tgccagtcgt tcaaatgcct
     5701 ctggcaaaag gcgcgtaact gtgtgagtca ttgtaaaaat ctccttaaat ctcttactca
     5761 tactacaatc ttaacatcta tttaacatat tgtcagtacc tcatttacat gatactatgg
     5821 ttgtaggggc tgatacaggt gctgtttcct ttcacaaaag gaaaattaat gcatgatttt
     5881 ttcacagcaa ttattgaatc tcatcgtttt agctcgttgg gctttgcggt atttattgtg
     5941 ctattggtgt tttgcgttat gaatcgagtc attacaaaaa agacaaagct tacaattcga
     6001 cttaaacttc ttcgtgttga gatagagatt tcagtaaata aatagctggt ggccttaccg
     6061 gcactttccc ttgataatat tggtggccct cagattaccg gggtcaccgt cttttaatcg
     6121 tagctggtgg actgaatccc ataaatcgta atgatacagc accgcaaact tcaaagcgtc
     6181 aatctttaaa tctgtcacat catcgacgaa ataataatcc ctcccttctt tcttcggtcg
     6241 aatccggtaa acttccccgt gagtatcttt catcaagtaa ccttcacgag aaatgctgta
     6301 acgtctgagt aaatagtcac ggtattttct ctcgccatta taagttttca ttaaaacatt
     6361 cctccaccat aaacacgtct gactttaact ttttctttct ttggctcggt gtgagttttc
     6421 ttttttggtg taaaagtaaa atcatcgtca ctatcatcaa agtcatcgag gtcactaacc
     6481 ttgttcttta gcttcgcatt tctgaatctt tctttaattt ctgctttacg ctgttcacgt
     6541 ttcaattcct tttcagggtc ttcttcatca tcaaggtcaa catccggtaa ttcatcatca
     6601 gtggcggggc actgtgtgtt tttatggtca ctactagcaa tcacatcaga gattttatct
     6661 tcctttttaa cgacctcttt agcagcttca ctatcaaggt catctaagtc agatgtttta
     6721 acagtctctt taatagcctc ttcaatacca agagaatcac attcatgtga atcacagagc
     6781 gaagctccct tcttaaaata ttcttcttca aagtcatctt ctttattgtt attaataagt
     6841 tctttcttgt tattactgtt attatacgtg tcaaagattt tgactgactg agtaaaaaat
     6901 tttgactcag tgggtaaaga attttgactc agtgggtcat ctttcttgac tgactgagta
     6961 actttagact ccagctttgc ctttctggct tccttagctg taagctctgt taaaacctcc
     7021 ggtacttcat caagtgaata aactttaata acacactgat tgcttgcaac gttagttttc
     7081 ttgattaaac catgtgcctc aagttgtttc atacatgcaa tcacggatgt tcttgtgatt
     7141 ccagcatact gaccaagatg agcatatgaa ggggtataat attgcccctt atcgtgataa
     7201 aacttttgtt cattcaacat gacagtgtaa ataatacggt cggcagcttt aacgccatta
     7261 gtcatctgat aaatacggtc atcaaacatg tggaattttt ccattatatt ttccttttta
     7321 aaaataaaac aaagaaaggg ctgaccacgt atagtgacca accccagagg gagtgaatta
     7381 attagaagat ggactgataa aggaactcag aagcctgagc gattacgcga gtattaacag
     7441 caaaaggaaa agccttgatt gaataagagg ccgtgaaaat ctgtcaatac cctgacgcct
     7501 cccaacgttg taacctctta atgttctgag ttcttttatc accccatata aaaactataa
     7561 cagatgtgat tataaattgc aacaattatt tcacagaata attactgatt ggtcatttat
     7621 tgttcacagg gatacttctt aatatctgaa tcacctatct tgtaaatgac catatttatg
     7681 agaattttag ctcaaccttt gagcaatcct gtatgacgtg ctcagaggca ttctgagagc
     7741 atctgcaatg cacttaatgg gttttccttg cctgtatagt tttatgaagc tgtcgagcgt
     7801 ctcaggtggg taaatggtgg gtcttcctct caagcctcct ctgttaatga tggttaacgg
     7861 taatagcgtg tcaccacagc aaaccgtctg tgtaatttcg tgcttacgtg gttctttttt
     7921 aaaaacaacg actgcactag gaaacggtga acgaatagta agcccgttat ggcctgctgg
     7981 tggaaggtat ttaactgcac gatgaagaat acggatttca tgacagtggt tattgctgta
     8041 aatgtgccta agccagtagc tggtgttggc tctatgcggt atcaggaaag ctactaaatc
     8101 cgcttcagac gcttttaaga ggaacgacgg aatatcgctg tacggcaggt tacagaagac
     8161 cttttcacta acccatgatt gacggcttat atcattggtg aatcgtggta acagcgtgtc
     8221 atgctcagtt gcagcaccat ctagcgtaaa accaaattca cggtgtaagt tcttaaataa
     8281 caacaatgga gttctccagt aatcagaacc cccatgatgt ttcccgatta aaatatgctc
     8341 aattgacata agaaataaaa aattttattg caatcactct tgacaaggtt tactctcttt
     8401 taagataaga gatacttaac aggctttcta aacggatatc ctttctggaa tatctatctc
     8461 ttatcttaaa agtctttaca gatactttaa agatatctgt attaaatctg tacagtaact
     8521 tttcagaatc tgaaaatttt tataaaatcg tagtagcacc tcttcaaatt ccccttctaa
     8581 aaatcttcga aaatatctga cagatgtctg tagctgctaa gtacccgtca caccagctat
     8641 gtgaccccct ttgcaaaaaa gccaaatatc attgactttc tcggtgaatt gctataaagg
     8701 catctatcta ggtctttaac tgtataaata cacatgacaa caaacgcccg ttaagtgtct
     8761 atataaaaag gtagctgtag ctatagacaa caattgtgat aattgattta atggctatag
     8821 acaagcaaga cagacctttg ttgactatat agagagataa gaacatgcgt acattccttt
     8881 atgctcgtgt atcagttgat gacctcacca cacagaacca agtagaagct gttaaacgtg
     8941 ctggttatga agtcagacct tcacgagtaa ttgaggaagt tattagtggg gctacaccag
     9001 ctatggacag accacaattt gtaaagctgg tggataagct ggaagaaggt gattgtcttg
     9061 tagtgaccaa gattgaccgc ttaggaaggg ataacattga cgtacagaag accgtcacaa
     9121 tgttacttgg tatgggtgtt aagctggtct gtctagattt gcctgtccaa gacctttcaa
     9181 gtgctgaagg taaacttatt cttcaactat tcagtacctt tgcagagttt gaacttaacc
     9241 gcatcagaga gcgaaccaga gagggcttag aacgagctaa agcacaaggt aaggtgttgg
     9301 gtcgtcctgt agctaaaaac actacagagg ccgttttaga gcataaggct aagggattgt
     9361 cacaaagtca ggttgcaaag cttctgaatc tctctgtgag aacggttagc cgtcactgga
     9421 caaagacact gtaaaaaaaa cttagtagca tcttcatagc ctccttcatg gggacttttt
     9481 tgtgtctgta acatatggtg accaaacatc tatatatcgt tatatagccc tgtatatagc
     9541 actatatata gtgtctggag agggacgact aacaccctgc atagttccct gaaaaggggc
     9601 ttcacagatt ctctatatat ttcaataagt taggataaaa agggactata ccgatactgc
     9661 atagaatctg tgtaggattg catagtcata ggggttggaa caagcccaac tacacctgat
     9721 aaagcctgtt attaacaggt agttgaatgt acgtgtttag ctatgtaaaa gcaccagaac
     9781 tttagccatt tctaaccact cctcgtgtta tctctattcc gtagcgattc gctaccactt
     9841 atttaaagat aagacgtcct tttcagagtg caaatttcac aaccacttat ttgatttata
     9901 acaactttca caagcacgta attttgtcgc aaaatgacac atttttatct catcgcgttt
     9961 ttttaatcat aagagcggct tatggataat tattggagat gatatctatt ctcgctaaga
    10021 agctgttgca ggatattacc aaacgcgggt ctacccgcgt cagttcacta agcttagtcc
    10081 cacgtagcga aaatatggca gccgccatac gccgcgttaa ttctatgcaa tatgatgtct
    10141 atacccagac ggaggtcagt aatggcaaac catcgaggcg gttccggcaa ttttgcagaa
    10201 gaccgcgaaa gagcatcaga agcaggtaaa aaaggtggac agcacagcgg gggtaatttc
    10261 aagaatgacc cgcagcgcgc atctgaagca ggtaaaaaag gtggcaagag cagtcacggc
    10321 aaaagcgaca actagccggg ctaatcaatg acgaatgcat ttttgtctgt agctcgtcaa
    10381 aaagccatca ccgccggtta cccggtggtt gatactgatg acaaatgtaa gcttgcctga
    10441 tgcgcgatgc ttatcaggcc taccagaaga ttgcaatata ttgaatttgc actgttttgt
    10501 aggccggata aggcgtttac gccgcatccg gcatgaacaa tgcgtacgtt gtcaacaatc
    10561 tgcaccgccg ggtaaccccg gcggttttct gtttatggct cctgatgaac aacttctggc
    10621 ggtggaacgt caaccaacct tctacttaac aacgcattga gtaaaatcgc gccaaaggtg
    10681 gctgtaccga tccctcccaa cgtaaaaccg cccagcgtga gagcaaaatc acccgcgccc
    10741 agcactaagg ttactgcgac cataatcaaa ttaccgttct ggcttaaatc gacacggttt
    10801 tgtacccaga tccttgcgcc tgcgacggca atcagcccga acacaacaat tgatgcacca
    10861 ccaatcaccg cggccggaat ggtatggatc agcgcgccaa atttcggcga aaagcccaac
    10921 agcatggcga tgacggcagc agcaacaaac accagcgtcg agtagacttt tgtcacggcc
    10981 atcacaccga tattttcagc ataggtggtg acgccgctac cgccgacaga gccggaaagc
    11041 atcgttgcca gaccatcacc gacgaatgct cgccccatat acgggtccat attgcgtccg
    11101 gtcatcccgg cgaccgcctt gagatgacct aagttttccg ccaccagaat caccgccacg
    11161 ggcgcaatca gcatcattgc ctgactatta aaagcaggag tggaaaaatg tggcaaaccg
    11221 aaccaggcag catggctgac gagagtaaaa tcgacggctt ttcccagccc taaaacgttg
    11281 gtcatcacgc catacagcag acaggcgaca attaaaccta cgagaatcaa taaccgctgg
    11341 atcatgccac gggtaaacac cgccaccagc ccaatacaca gcaccgtcat taccgccatc
    11401 cagctatcaa aggctgaagc cgatacactt ttcactgcga taggcgctaa gttcaggcca
    11461 atcgccatca ccaccgcacc cgtcaccacc ggcggcatta gtcgttcaat ccagcgtgta
    11521 ccaattttca tcaccaccag gccaatgacg gtataaacca gcccacaggc gataatcccg
    11581 cccagtgcga tgctgatatt cgggttaatg ccctgaccgt taaagcccgt cgcggcaatc
    11641 accacgccga caaaagccgc gctggagccg agataactgg ggacgcgccc gccggtaata
    11701 aagaaaaaca gtaacgtgcc gatccccgac attaaaatgg aaagattggg atccagcccc
    11761 atcagaatcg gcattaacac cgtcgcgcca aacatcgcca ccgcgtgttg aacgcccatt
    11821 actgccgtct gagcaaacgg caatcgttca tccggcgcga ccacgccgct ctctgtagag
    11881 gtcgatttta actgccagtg aggaaaaccg aacattgcca tcagctgtct ccttaaggag
    11941 gttaacaagc agggcgcatc agcgcgtgat aactgcgatc gaaccacacc agcccgtagg
    12001 gtgtggtgtg acgatgaatc gcttcgatgg cgcaaaacag aatgtcgtgg gtgccgacgc
    12061 tcaccacctg gctgatgcgg cagtcaaacg aaaccagagc ctcttccagt tgcgggcatc
    12121 cggtcacacc cgtctgccag cgggcggcgg caaagcggtg ttccatgggc gttttgccgc
    12181 caaaaagatt tgaaagcggc tcctgcccgg cgctcagtgt atttacacac agcgttcgat
    12241 tttcattgaa taccggccag acggacgccc cacgattcag gcacaccagt aatgtgggcg
    12301 gcgtgtcggt cacgctgcag acggcgctgg cggtgaaccc ggcgcgcccg gatggaccgt
    12361 ccgtggtgat gatattgacc gccgcgccca tacaggacat cgcatcgcga aaggtttgtt
    12421 gatcaactat gttcatagtt tgctccttac aacagcccgc aggcttcttc aaaggacaga
    12481 cgtggcaggc gcgcaaaaag tttgctgcta tcgccatagc cgatattaat cagcagattg
    12541 ctcttcagcg tgctgcccgc aaaaaaggcg tcgtcaacgt gttgacggtc aaaacccgac
    12601 atcgggccgg tatccagtcc cagcgcacgg caagcgacga tcagataggc cgcctgcatg
    12661 gagctgttgc gaaacgctgt ttcttcggca agttgtgggc tggaggtaaa ccaactgcgg
    12721 gcatcaccgt ggggaaacag tagtggtaac cgttcataaa attcactgtc ccaggcaacg
    12781 atagcggtga cgggcgcggt cagggttttt tgtagattgc cgctggaaag cgccgggtgc
    12841 agacgttctt ttccttctac agtgcggata aacacgatcc gtgccggaga acagttagct
    12901 gatgtcggcc cccattttat cggggcataa agctcccgta acgtctcatc gctgacgggt
    12961 gtctcccgcc agccgttgtg agtgcgggca tcggtgaaca gggtgctaag cgcacctggg
    13021 ctaacggctt cgttcatagc aattccttac agggcggctt cacggtgatg taacaggctg
    13081 gcaagcccgt tgagtaacag agcattaaac gtttcggggt cggtcacgtt gcaggcgtgt
    13141 ccgccatagc gcatcaccat tttctggcta tcgggcaggg cggcatgaag ctcactggaa
    13201 catgctgatg gcaccagcag atcatcactg gcgcagatga tttgcaccgg gcagcggatg
    13261 cgatccgcat ggtgactaaa gtcagcgcgt ttgagggcgt taagtcgacg cagtaaatta
    13321 tttttgccct gaaaatgcgc cagtgccagc gcgtcttctg cctcaaggcg aggtgcgcgg
    13381 gccgccatcc agtcggcggg atagaggaac aacggctgcg cttccaccca tgcctgcgcg
    13441 ccgccgctat acagtaatcg ttcgcgaacc tgaaaacagc ggcgcgtatg ggcgtttatt
    13501 cgtagccagc cgttaacgct gatcagcaca gttaccgacg cgggataatc cagcgccagc
    13561 tgcattccca ccagcgcacc gagcgcatgg ccgaccaccg cgtaatgctc aatccctgcg
    13621 gctaccagcg cctgatgcag ttccgctgcc atctgggcga tactgtaatc ttctgccagc
    13681 gtgtcgggat tattgccggt gccgcgctgg tcgtaacaga ctacctgata ctcctgctcc
    13741 agcaccgcca gttgcggtaa ccagtaactg ccgctaccgc caagacccga aatcaacacc
    13801 actacgggcg catcagcata agggggaggt gagagtgaaa gtttcatcgc ggcctcactt
    13861 ggcgatatgc gcaattgtgg cgatttccac cagcgcgtca ggttttacca gtccgcactg
    13921 aatgcagaat cgcgccggtt tatcacccgg aaaaaactcg gcgtagattt cgttaatcgc
    13981 ggcgtaattt ttccagtcgg taataaagat gctgttgaag gtcacatccg ccatcgtgcc
    14041 acccgccgtc tcgatcacct tgcggatagt ttccagaacg tggcgggttt gcgcctttgg
    14101 gtcatcggca aacagcacgt t
//
LOCUS       DAJTVK010000106        14108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000106 DAJTVK010000000
VERSION     DAJTVK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.106"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(14..1372)
                     /gene="hprS"
                     /locus_tag="OO372_003182"
     CDS             complement(14..1372)
                     /gene="hprS"
                     /locus_tag="OO372_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6342601.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1372..2043)
                     /gene="hprR"
                     /locus_tag="OO372_003183"
     CDS             complement(1372..2043)
                     /gene="hprR"
                     /locus_tag="OO372_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6342602.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2176..2589
                     /gene="hiuH"
                     /locus_tag="OO372_003184"
     CDS             2176..2589
                     /gene="hiuH"
                     /locus_tag="OO372_003184"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6342603.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2698..3702
                     /gene="msrP"
                     /locus_tag="OO372_003185"
     CDS             2698..3702
                     /gene="msrP"
                     /locus_tag="OO372_003185"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR6342604.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3703..4338
                     /gene="msrQ"
                     /locus_tag="OO372_003186"
     CDS             3703..4338
                     /gene="msrQ"
                     /locus_tag="OO372_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6342605.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4595..5245
                     /gene="zinT"
                     /locus_tag="OO372_003187"
     CDS             4595..5245
                     /gene="zinT"
                     /locus_tag="OO372_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6342606.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5588..6118
                     /locus_tag="OO372_003188"
                     /pseudo
     CDS             5588..6118
                     /locus_tag="OO372_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6688..6777)
                     /locus_tag="OO372_003189"
     tRNA            complement(6688..6777)
                     /locus_tag="OO372_003189"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6741..6743),aa:Ser,seq:cga)
     gene            6871..7668
                     /gene="mtfA"
                     /locus_tag="OO372_003190"
     CDS             6871..7668
                     /gene="mtfA"
                     /locus_tag="OO372_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6342607.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7769..7844
                     /locus_tag="OO372_003191"
     tRNA            7769..7844
                     /locus_tag="OO372_003191"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7802..7804,aa:Asn,seq:gtt)
     gene            8158..14108
                     /gene="yeeJ"
                     /locus_tag="OO372_003192"
                     /pseudo
     CDS             8158..14108
                     /gene="yeeJ"
                     /locus_tag="OO372_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3990 a         3354 c         3171 g         3593 t
ORIGIN      
        1 ctgaagccct atgttaattt ctttgcggta acataatccg gaacacatta tgcttattga
       61 gatagtgata cgtagcactt cccccatgta attcggcaat cgctttgact aaagaaagac
      121 caagtccctg acctacggaa tgacgcgaat tatctccccg ccaaaatcta cggaagagtt
      181 tttcaggctc atgaattttc gttccaggac tggcgacatc aatattaaga tagccgttgg
      241 tatcaagaaa actggttata tgaatacgcg atttttctgg tgaatatcta atggcattaa
      301 caataagatt cgataacatt cgttgcagta aaattttatc cgcaaagatt tgctgattgc
      361 actcgacctt aaagcaaatc tctttctcgt ctgaaaggta ttcaagataa tccaacaaat
      421 tttcgacttc cttattgaga gaaagcgagt ccagtttcac caaaacattg tttttatctg
      481 cccgggcaag aaacagtatg ttctctgtta accgcgaaat attttccagc tcttcaatgt
      541 taccggcaat tgttttttga tattcagcga tacttctggt ttgactgagc gtaacctgat
      601 tctgacccag taatgcatta atgggcgttc taagttcatg agcgagatcg tcagcaaatt
      661 gacttagacg ctcaaaatct ttgactaaag catgatgcat tttattcaac gcctgcccaa
      721 gaggttttag ttctctcggt aatgcgctaa cctcaacagg ctcccggcta tcgttataat
      781 tcagcgcttc cgttacacca ctcaactttt tgatctctcg taatcccgtt ctgattaaca
      841 gcggacttaa tactgagcaa agtacaatgg cgacaatgca aattataatg ctattaattt
      901 tatactgttc aagcatgtta tgtctggctg aagccaattt agccacagta accgttaatg
      961 atggcgaaac ttcatcaata ttaattcgta aagcatcaat ctcagtatca ttaataatgc
     1021 ttctgtaaat gccagctgcg ctgattgtct cactagcagg tatgttattt aacatgtcat
     1081 cactgacatt tgtccggtta acaattttat tgatgccatc accatgaatg atcaagatat
     1141 cctgactaac atccatcatc cggttaaagt atacaggtaa cgtatctgga tttaccccat
     1201 caattaacaa ctgcttgatc tgtgctgtcc ggttaatgag tgttgtatca tcgcgccatt
     1261 tcaactcact tgccaggcca ttatagagag tccagacaat tccagcgcca gcaacagaca
     1321 gcagcaatat aaaaagcaag gttaaacgga cggttataga taatcttttc attatttttt
     1381 taccgctacg aatgaatagc ccatcccccg gattgtggcg attagctttt caggaaaagg
     1441 atcatcaact tttgcgcgga gcctgcgaat ggcgacatcc accgtattgg tatcactatc
     1501 aaagttgatt ccccaaattt cactcgcaat aaccgttctg ggtataattt cgccagctct
     1561 ggaggccagt agccaaagta actgaaactc cttgcgcgtc agtgtaatac tgatattgtt
     1621 cctgctcaca ctttgactta cagagtccat tcttaagcca ctgatttcta atgttgaatt
     1681 caaagcgtga tgttgcctta attgtgcccg aacccttgcc agcaactcag aaaatgaaaa
     1741 aggttttacc agataatcat ttgctccact gtccagccct ctgactctgt catcgacaga
     1801 atcccttgca gtaaggcaaa taacaggggt ttgctttgct gttcttaacg tttgtaagat
     1861 ctgccagcca tccatacccg gaagcataat atccagaatg atcaatgcat aatcatcctt
     1921 cagcgcaaga taaagcccat ctctgccatc agaaacggca tcaatgacat aacccgcttc
     1981 ggaaagcccc tgcgttaccc actcctgggt cctttgatta tcttcaataa gtaaaatctt
     2041 catgaaaata atatgccata aatcatatgt tatagaaaca gcctagttca ttacaaaatt
     2101 gtaatgctgc tgtaaggtta ccctggccgc tttttcgcta tcctcaaaac tcattcacat
     2161 gacaaggata taaacatgtt aaagcgttat ttagtactct ccgtagtaac ggcagcattt
     2221 tcattacctt ctttggttta tgccgcacaa caaaacattc ttagcgtgca cattttgaac
     2281 cagcaaaccg gaaaacccgc tgccgacgtg acagtcactc ttgaaaagaa agcggacaac
     2341 ggctggttac aacttaatac cgccaaaaca gataaggatg gacgaattaa ggcactgtgg
     2401 cccgagcaaa ctgcaactac gggcgattac cgtgtcgtat ttaaaaccgg ggactatttc
     2461 aagaaacaaa atcttgaaag tttcttccct gagatccccg ttgaatttca tattaataaa
     2521 gtgaacgagc attatcatgt gcctttatta cttagccaat atgggtattc aacctatcgt
     2581 ggcagttaat gcataaatat gtaaaagcag atctctgcta ctgttaggga ggacactccc
     2641 tgacagatta acagtaaacg gctcttgctg gctaacgaca aaaaagtgtg atggcttatg
     2701 aaaaagaatc aatttttaaa agaatcagat gttacggccg agtcggtatt ctttatgaag
     2761 cgtcgacagg tgttaaaagc actgggcatc agcgcagctg cactttcttt gcctcacgct
     2821 gcgcatgccg atctgcttag ctggtttaaa gggaacgatc gcccgcccgc ccccgccgga
     2881 aaaccgctgg agttcagcaa gcctaccgcc tggcaaaata atctgccact gacgccagta
     2941 gataaagtct ccggttataa caacttctat gaattcgggc tggataaagc cgatcccgcc
     3001 gctaatgctg gtagcctgaa aaccgatcca tggacactga aaatcagcgg cgaagtggca
     3061 aaaccattga ccctcgatca cgatgattta acccgtcgct tcccgctgga agagcgtatt
     3121 tatcgtatgc gctgcgtgga ggcatggtcg atggtggtgc cgtggattgg ttttccgctg
     3181 cacaaattgc tggcgcttgc cgaacccacc agcaatgcga agtatgtcgc tttcgaaaca
     3241 atttatgcac cggaacaaat gccaggccag caggaccgct ttatcggcgg cgggctgaaa
     3301 tatccttatg tcgaaggatt gcgtctcgac gaagcaatgc atccgctcac actgatgacc
     3361 gtgggtgttt atggcaaggc gttaccgcca caaaatggcg cgccggtacg actgattgtg
     3421 ccgtggaaat atggctttaa agggattaaa tcgatagtca gtattaagct gacccgcgag
     3481 cgtccgccaa ccacctggaa tctggcagcg cctgacgaat acggttttta cgccaacgtt
     3541 aatccgcatg ttgatcaccc gcgctggtca caggctaccg aacgatttat tggttcaggc
     3601 ggcatcctcg atgttcagcg ccagccaacg ctactgttta atggttacgc cgaccaggtg
     3661 gcatcgctgt atcgtggcct ggatttgcgg gagaatttct gaatgcgtct gacagcaaaa
     3721 caggtgacat ggctgaaagt tagcctgcat cttgccggat tgttgccgtt tctctggctg
     3781 gtctgggcga tcaatcacgg tggactgggt gccgatccgg tgaaagatat tcagcatttt
     3841 accggtcgca ctgcactgaa atttttgctg gcgaccttgt taatcacacc tctggcacgc
     3901 tacgcaaaac agccgttatt gatacgcact cgccgcctgt taggcttatg gtgctttgcc
     3961 tgggcgacac tgcatttaac cagttacgca ttgctggagc tgggcgtgaa taatctggcg
     4021 ttattaggca aggagttaat tacccggcct tatttaacgt taggaattat cagctgggtt
     4081 attttgcttg ctttagcgtt tacttcaacc caggcgatgc agcgaaaact cggcaagcac
     4141 tggcaacagt tgcataattt cgtctatctt gtcgcgatcc tggccccaat acattatctg
     4201 tggtcagtga agattatttc accgcagccg ctcatctacg ctgggctggc tgtactgctt
     4261 ttagccttac ggtataagaa gttgcgttcc ctttttaagc ggttacgcaa gcaggttcac
     4321 aacaaattat ctgtataact tcatattgcc gacaaagtac gcgttatccc tgccggatgt
     4381 ggcgcgagcg ccctgtccgg cctgcgcaga tcacatcagg ttatgtttcg tttgtcatca
     4441 acctcaaatt atcaacataa ttcattttat ttcaataaga tagataagta cacctgagaa
     4501 aactatgctc tcatctccta agagttgttg cattttgcca tatgttacaa tataacatta
     4561 cacatcatat acaatgactc tggaggaaac tgttttggca attcgtcttc acaaactggc
     4621 tgttgcttta ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc atcactcaca
     4681 cggcaaaccc ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg atgatgccaa
     4741 tgtacaaaac cgaacgctca gtgactggga tggagtctgg caatccgttt atcctttact
     4801 gcaaagtggc aaacttgacc ccgtctttca gaagaaagcg gatgcagata aaattaaaac
     4861 atttgctgaa attaaagatt attatcacaa aggttatgca acagatatcg agatgattgg
     4921 cattgaggac ggcattgttg aattccatag aaataatgaa acaacatcct gtaaatatga
     4981 ttacgatgga tacaaaatac tcacctataa atcaggcaag aaaggcgttc gttacttatt
     5041 cgaatgtaaa gatcctgaaa gcaaagcccc taaatatata caatttagcg atcatataat
     5101 tgcaccacga aaatcatctc attttcacat ttttatgggt aatgattcac agcaattttt
     5161 attaaatgaa atggaaaact ggccaacgta ttatccatat cagttgagta gcgaagaagt
     5221 ggtcgaggaa atgatgtctc attgagtatt ctcatgataa cgcctcgatg ccgctttagt
     5281 aagtcatcat aactgccact ggtccatcca caaacgccac tgaacgcaag ctagctacag
     5341 acacgctcat cactatgacg tgtctgtata ttaataagct aacccgcatt gagttaacca
     5401 ataacggatt ccatacacaa tacggccaac agagaaagat acctgtgctc acgccattgc
     5461 ttatattggc tggttacaat gtgcactatc aattttttaa ataacaaatt cttaaaacca
     5521 catctgtata aggaattttt aaggttcgtg ggatagcttg actgtaaaaa ttacaggagc
     5581 tacaaaaatg aaccgattct caaaaactca aatttattta cattggataa cgctgctttt
     5641 cgttgcaata acctatgccg cgatggaact ccgtggctgg tttcctaaag gtagtagtac
     5701 ttatctgctg atgcgagaaa cacattacaa tgcgggtata ttcgtttggg tgttaatgtt
     5761 ttcacgcctg attataaaac accgttatag tgatccttct attgtaccac cgccacctgc
     5821 ctggcaaatg aaagcggctt cgctaatgca catcatgctt tatataacct tccttgcact
     5881 tcctctgctg gggattgctt tgatggctta cagtggaaaa tcgtggagtt tccttggttt
     5941 caatgtgtct ccctttgtta ccccaaacag cgaaattaaa gcactgataa aaaatattca
     6001 cgaaacctag gcaaatatag gttacttttt aatcgcagct cacgctggcg cagcactctt
     6061 tcatcactac attcagaaag ataatactct gttacgaatg atgcatcgcc gcaaataagt
     6121 cgatcccctg acaaggaaat gtgaaccatg tacatcaacg cgtcataaat gtttacgcaa
     6181 gtgcacaatg catcctttct actatcaaca aggaggagca gaaactgtgt aaatcgtaca
     6241 atggtaagac ttatcaaaaa tacaggttaa ttttggtatt taaacctttt ttacttgtta
     6301 gtgtgataca aaggctttca aaaaagctgg gaaattcaac aactcttgga aattatggac
     6361 tttgtcccgc ggctttaatc tgacggccgc gttccttttt tgactcaatt attgacccct
     6421 tctctacgca acttcagttc ccaccaccaa ctttgcggca gtttcatagg atgaatgtct
     6481 aaaagaataa tggtgagcga taagaaaacg actgaataac tgcacatttt cgctcgaaag
     6541 cttcctgtca gatccatagc gaatcaagtg ctgaatgtca cagtatcgaa cagaaaacag
     6601 tgacgatcta acccttcaag aatattctac aattgttctg tttaggaaaa gcaaggcggg
     6661 aagtcaggag ataagtcatt gataaagtgg cggagagagg gggatttgaa cccccggtag
     6721 agttgcccct actccggttt tcgagaccgg tccgttcagc cgctccggca tctctccgtt
     6781 cagatggttg ccatgatgcc aggaaatttg gcattttaac agtccctgtt cgtgcaattt
     6841 tgttcaagtg acgagtttgc gagcaaaacg atgattaagt ggccctggaa agtacaagaa
     6901 tcagcacatc aaactgccct tccctggcag gaagcactat cgatccccct tttaacgggt
     6961 ctgacggaac aggaacaaag caaattagtc actcttgccg aacgtttttt acagcaaaag
     7021 cggcttgttc ctttacaggg ctttgaactg gattcattaa gaagctgccg gatagcactt
     7081 ctattttgcc tacccgttct ggagttagga ctggaatggc tggatggttt tcatgaagtc
     7141 ttaatttatc ctgcgccatt tgtggtcgat gatgaatggg aagacgatat cggtctggtg
     7201 cataaccaac gtattgttca gtcaggtcag agctggcagc aagggcctat cgttttgaac
     7261 tggttggata tacaagattc ttttgatgca tctggtttta acctgattat tcatgaagtc
     7321 gctcataagc tggacacccg taacggcgat cgcgccagcg gagttccctt tattccgttg
     7381 cgtgaggttg ctggctggga acacgatctt catgctgcaa tgaacaacat tcaggaagaa
     7441 atcgaattgg ttggcgagaa tgcggcgagc attgatgctt atgcagccag cgatcctgct
     7501 gaatgttttg ccgtactttc tgaatatttc tttagcgccc cagaactttt tgctcctcgt
     7561 ttcccttcat tgtggcaacg tttctgccaa ttttatcaac aagatccttt gcagagactg
     7621 catcacgcta atgatacaga ctcgttttcg gcgacgaatg ttcattaatt aacaactttg
     7681 cagattaatt aaccaattga aatgacttat gaaatttagt gttgacagac aaggtaccgc
     7741 taagtaatat gtgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     7801 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattct aaaaattcgc
     7861 ttttttagcg caatgtcact gaccttagtt gaacattgtt ttttaacgga tagcgggttt
     7921 ttaacacctt aagcgccctc gacctttatg gttgagggcg ttttgctatg aacgccatca
     7981 ccattttccc ctcgattata aaacttgagt tattcagtag tctcccctct tgcaactcac
     8041 acccaaaact gcctaacgaa aagttattaa ttttcaatcg tattgctatc agtatttaca
     8101 ttttttcgct gtgctagaaa gggcgcattt atgttagctc gttcagggaa ggtaagcatg
     8161 gctacgaaga agagaagtgg agaagaaata aatgaccgac aaatattatg cgggatggga
     8221 attaaactac gccgcttaac tgcgggtatc tgtctgataa ctcaacttgc gttccctatg
     8281 gctgcggcag cacaaggtgt ggtaaacgcc gcaacccaac aaccagttcc tgcacaaatt
     8341 gccattgcaa atgccaatac ggtgccctac acccttggag cgctggaatc ggcccaaagc
     8401 gttgccgaac gtttcggtat ttcggtggct gagttacgca aactcaacca gtttcgtacg
     8461 tttgctcgag gttttgataa tgtccgccag ggtgatgaac tggatgtccc ggcacaagtt
     8521 agtgaaaaaa aattaacccc gccgccgggt aataacagtg acaacctcga gcaacagata
     8581 gccagtactt cacagcaaat cgggtctctg ctcgccgaag atatgaacag cgagcaagcg
     8641 gcaaatatgg cgcgtggatg ggcctcttct caggcttcag gcgcaatgac agactggtta
     8701 agccgcttcg gtaccgcaag aatcacgctg ggcgtggatg aagattttag cctgaagaac
     8761 tcccagttcg attttctcca tccgtggtat gaaacgcctg ataatctctt tttcagtcag
     8821 catactctcc atcgtactga cgagcgtacg cagattaaca acggcttagg ttggcgtcat
     8881 ttcactccca catggatgtc gggcatcaac ttctttttcg accacgatct tagccgttac
     8941 cactcccgcg tcggcattgg cgcggagtac tggcgcgact atctaaaatt aagcagtaac
     9001 ggctatttgc gactgaccaa ctggcgcagc gcacctgaac tggacaacga ttataaagca
     9061 cgcccggcca atggctggga tgtacgcgca gaaagctggc tacccgcctg gccgcacctt
     9121 ggcggtaaac tggtctatga acagtattat ggcgattaag tggccctgtt cgataaagac
     9181 gatcggcaaa gtaatcctca tgccataacc gctggactta actatacccc cttcccgctg
     9241 atgaccttca gcgcggagca acgccagggt aaacagggcg aaaatgacgc ccgttttgcc
     9301 gtcgatttta cctggcaacc tggcagcgca atgcagaaac agcttgaccc gaatgaagtc
     9361 gctgcacggc gtagccttgc aggcagccgt tatgatctgg tggatcgcaa caacaatatc
     9421 gttctggaat atcgcaaaaa agaactggtt cgcctaaccc tgacagaccc cgtgacaggg
     9481 aagtcaggag aagtgaaatc actggtttcg tcgctacaaa ccaaatatgc cctgaaaggc
     9541 tataacgtcg aagccaccgc actggaagct gccggtggca aagtggtcac aacgggtaaa
     9601 gatattctag ttaccctgcc ggcttaccgg ttcaccagta cgccagaaac cgataacacc
     9661 tggccgattg aagtcaccgc cgaagatgtc aaaggcaatt tgtcgaatcg tgaacagagc
     9721 atggtggtcg ttcaggcacc tacgctaagc cagaaagatt cctcggtatc gttaagtacc
     9781 caaacattga acgcggattc ccattcaacc gccacactga cttttattgc gcatgatgca
     9841 gcaggtaatc ctgttgtcgg gctggtgctc tcgacgcgtc acgaaggtgt tcaggacatc
     9901 accctttctg actggaaaga taatggtgac ggaagctata cccagatcct gaccacagga
     9961 gcgatgtctg gcacgctgac gctgatgcca cagctgaatg gtgtggatgc ggctaaagcc
    10021 cccgccgtgg tgaatatcat ttctgtttcg tcatcccgaa ctcactcgtc aattaagatt
    10081 gataaggacc gttatctctc cggcaatcct atcgaggtga cggtagaact gagagatgaa
    10141 aatgacaaac ctgttaagga acaaaaacag caactgaata acgcagtcag catcgacaac
    10201 gtgaaaccag gagtcactac agactggaaa gaaaccgcag atggcgtcta taaggcgacc
    10261 tataccgcct ataccaaagg cagtggactt actgcgaagc tattggaatg aagatttgca
    10321 taccgctgga tttatcatcg acgccaaccc gcagtcagcg aaaattgcga cattatctgc
    10381 cagcaataat ggtgtactcg ccaatgagaa tgcagcaaac accgtctcgg tcaatgtcgc
    10441 tgatgaagga agcaacccaa tcaatgatca taccgtcacg tttgcggtat taagcggatc
    10501 ggcaacttcc ttcaacaatc aaaacaccgc aaaaacggat gttaatggtc tggcgacttt
    10561 tgatctgaaa agtagtaagc aggaagacaa cacggttgaa gtcacccttg aaaatggcgt
    10621 gaaacaaacg ttaatcgtca gttttgtcgg cgactcgagt accgcgcagg ttgatctgca
    10681 gaagtcgaaa aatgaagtgg ttgctgacgg caatgacagc gccacaatga ccgcgaccgt
    10741 ccgggatgca aaaggcaacc tgctcaatga cgtcatggtc actttcaatg ttaattcagc
    10801 agaggcgaaa ctgagccaaa ccgaagtgaa tagccacgac gggatcgcca cagctacgct
    10861 gaccagtttg aaaaatggtg attatagggt tacggcctct gtgagctctg gttcccaggc
    10921 taatcaacag gtgaatttta tcggtgatca aagtactgct gccctgaccc tcagtgtgcc
    10981 ttcaggtgat atcaccgtca ccaacacagc tccgcaacat atgactgcaa ccttgcagga
    11041 taaaaatggc aacccactaa aagataaaga aatcaccttc tctgtgccaa acgacgtcgc
    11101 aagtaagttc tcgattagca acggaggaaa aggcatgacg gatagtaacg gggttgcaat
    11161 cgcctccctg accggcacgt tagcgggcac gcatatgatc atggctcgtc tggctaacag
    11221 caatgtcagc gatgcacagc caatgacgtt tgtggcggat aaagacagag cggttgtcgt
    11281 tttgcaaaca tcgaaagcgg aaatcattgg gaatggcgtg gatgagacaa ctctgacagc
    11341 aacagtgaaa gatccgtcga atcatccggt ggcggggata acggtaaact tcaccatgcc
    11401 acaggacgtt gcggcaaact ttacccttga aaataacggt attgccatca ctcaggccaa
    11461 tggggaagcg catgtcacgc tgaaaggtaa aaaagcgggc acgcatacgg ttaccgcaac
    11521 gctgggtaat aacaatacca gtgattcgca gccggtaaca tttgtggcgg acaaagcctc
    11581 ggctcaggtt gtcctgcaga tatcaaaaga tgagatcaca ggtaatggcg tcgatagcgc
    11641 aacgctaact gcaacggtta aagatcagtt cgacaatgag gtgaataatc ttccggtaac
    11701 attcagctca gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc
    11761 tggcatcgcg caggccactc tcgcaggcgt tgcctttggt gagcagacgg tcactgcatc
    11821 actggctaat aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc
    11881 ggcaaaaatt atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa
    11941 cagctccggc agcgccatca ccgccacagt cgttgataat aatggctttc cggtgaaagg
    12001 tgtgactgtg aacttcacca gcagaacaaa ctctgccgaa atgacgaatg gcggccaagc
    12061 cgtaacgaac gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga
    12121 atcaggagcg agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag
    12181 cacatcaatt aatgtcaacg ctgatgcgtc tacggcacat ctcacctggc tacaggcact
    12241 ttttgataca gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta
    12301 cggcaacggt gtcccccagc atcaggtgac cctcagcgtt tcaccaagtg aaggcgtgac
    12361 ccccagtaat aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac
    12421 cgctacaaaa gccggggttt atcaagtgac ggcaaccctc gaaaatggcg attcgatgca
    12481 acaaacagtg acctatgtgc cgaacgtcac gaatgccgaa atcacgctgg caacctcgaa
    12541 ggatccggtg attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac
    12601 agagggcaat gcgatagcca acactgaagt aacatttact ctgccggaag atgttaaggc
    12661 gaacttcacg ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt
    12721 cacgctgaaa ggtacaaaag caggcgctca tactgttaca gcatcgataa ctggcggtaa
    12781 gagtgagcag ttggtggtga actttactgc ggatacgctc actgcgcagg ttaatcttaa
    12841 cgttaccgag gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt
    12901 gactgatgga aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga
    12961 tgtgagcgca agctttactc tcggacaagg cggttccgcc attactgaca tcaacggcaa
    13021 ggctgaagtt acactgagcg gtacaaaatc cggcacctac cccgtgacag ttagcgtgaa
    13081 caattatggt gtcagtgata cgaaacaggt gactttgatt gccgatgctg gtaccgcaac
    13141 actagcctcc ttaacctctg tatactcatt cgtcgtcagc acgaccgaag gcgcgaccat
    13201 gactgcaagc gtcactgacg ctaacggcaa cccggtagaa ggcataaaag ttaatttccg
    13261 cggaacctcc gtcacgctaa gcagcaccag cgttgaaacg gatgatcggg gtttcactga
    13321 aattcttgtg acaagcaccg aagtcggact gaaaacagtt tcagcctctc tggcagataa
    13381 acctactgaa gtcatatcgc gattactgaa tgcaaaagca gatattaatt ctgcaacgat
    13441 taccagtctg gagatacctg aaggtcaggt catggtcgca caagacgtag cagttaaagc
    13501 tcacgtcaac gaccagtttg gcaacccgat tcttaatgaa tctgtaacat tcagtgcaga
    13561 gccaccagag cacatgacca tcagccaaaa tattgtctct actgatacgc atggtatagc
    13621 cgaggtctcc atgacgcccg aaagaaacgg ttcgtatatg gtgaaagcat ccctggcgaa
    13681 tggagcctca cttgagaaac aactggaggc tattgatgaa aaactgacac tctcggcgtc
    13741 cagcccgctt atcggtgtca attccccaac aggtgcaact ctgacggcaa cgctaacttc
    13801 tgcaaatggc actccagtgg agggtcaggt catcaacttt agcgtaacgc cagaaggtgc
    13861 gacgttaagt ggcggaaaag tgagaactaa ctcttctggt caggctccag tcgttctgac
    13921 cagcaataaa gtcggtacat atacggtgac tgcatcgttc cataacggcg taacaataca
    13981 gacacagaca accgtgaaag tcactggcaa ctcaagcacc gcccatgtta ctagctttat
    14041 cgctgatcca tcgactatcg ccgccaccaa cagtgattta agtaccttaa aggcaacggt
    14101 tgaggatg
//
LOCUS       DAJTVK010000107        14092 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000107 DAJTVK010000000
VERSION     DAJTVK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14092
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.107"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..2217
                     /gene="ldcC"
                     /locus_tag="OO372_003193"
     CDS             76..2217
                     /gene="ldcC"
                     /locus_tag="OO372_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6342608.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2274..2663
                     /locus_tag="OO372_003194"
     CDS             2274..2663
                     /locus_tag="OO372_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6342609.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2728..4026
                     /gene="tilS"
                     /locus_tag="OO372_003195"
     CDS             2728..4026
                     /gene="tilS"
                     /locus_tag="OO372_003195"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6342610.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4075..4335)
                     /gene="rof"
                     /locus_tag="OO372_003196"
     CDS             complement(4075..4335)
                     /gene="rof"
                     /locus_tag="OO372_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6342611.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4322..4522)
                     /locus_tag="OO372_003197"
     CDS             complement(4322..4522)
                     /locus_tag="OO372_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6342612.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4688..5233
                     /locus_tag="OO372_003198"
     CDS             4688..5233
                     /locus_tag="OO372_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6342613.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5230..5591
                     /gene="arfB"
                     /locus_tag="OO372_003199"
                     /pseudo
     CDS             5230..5591
                     /gene="arfB"
                     /locus_tag="OO372_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5665..6375
                     /gene="nlpE"
                     /locus_tag="OO372_003200"
                     /gene_synonym="cutF"
     CDS             5665..6375
                     /gene="nlpE"
                     /locus_tag="OO372_003200"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6342614.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6530..7354)
                     /locus_tag="OO372_003201"
     CDS             complement(6530..7354)
                     /locus_tag="OO372_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6342615.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7407..9125)
                     /gene="proS"
                     /locus_tag="OO372_003202"
     CDS             complement(7407..9125)
                     /gene="proS"
                     /locus_tag="OO372_003202"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6342616.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9236..9943)
                     /gene="tsaA"
                     /locus_tag="OO372_003203"
     CDS             complement(9236..9943)
                     /gene="tsaA"
                     /locus_tag="OO372_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6342617.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9940..10344)
                     /gene="rcsF"
                     /locus_tag="OO372_003204"
     CDS             complement(9940..10344)
                     /gene="rcsF"
                     /locus_tag="OO372_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6342618.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10462..11277)
                     /gene="metQ"
                     /locus_tag="OO372_003205"
     CDS             complement(10462..11277)
                     /gene="metQ"
                     /locus_tag="OO372_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6342619.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11317..11970)
                     /gene="metI"
                     /locus_tag="OO372_003206"
     CDS             complement(11317..11970)
                     /gene="metI"
                     /locus_tag="OO372_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6342620.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11963..12994)
                     /gene="metN"
                     /locus_tag="OO372_003207"
     CDS             complement(11963..12994)
                     /gene="metN"
                     /locus_tag="OO372_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6342621.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13182..13757
                     /gene="gmhB"
                     /locus_tag="OO372_003208"
     CDS             13182..13757
                     /gene="gmhB"
                     /locus_tag="OO372_003208"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6342622.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3360 t
ORIGIN      
        1 tcacttcggt ggcccttttt tatcgccacg gtttgagcag gctatgatta aggaaggatt
       61 ttccaggagg aacacatgaa catcattgcc attatgggac cgcatggcgt cttttataaa
      121 gatgagccca tcaaagaact ggagtcggcg ctggtggcgc aaggctttca gattatctgg
      181 ccacaaaaca gcgttgattt gctgaaattt atcgaacata accctcgaat ttgtggcgtg
      241 atttttgact gggatgagta cagtctcgat ttatgcagcg atatcaatca acttaatgaa
      301 tatctcccgc tttatgcctt catcaacacc cactcgacga tggatgtcag cgtgcaggat
      361 atgcggatgg cgctctggtt ttttgaatat gcgctggggc aggcggaaga tatcgccatt
      421 cgtatgcgtc agtacaccaa cgaatatctt gataacatta cgccgccgtt cacgaaagcc
      481 ttgtttacct acgtcaaaga gcggaagtac accttttgta cgccggggca tatgggcggc
      541 accgcatatc aaaaaagccc ggttggctgc ctgttttatg attttttcgg cgggaatacc
      601 ctcaaggctg atgtctctat ttcggtcacc gagcttggtt cgttgctcga ccacaccggg
      661 ccacacctgg aagcggaaga gtacatcgcg cggacttttg gcgcggaaca gagttatatc
      721 gttaccaacg gaacatcgac gtcgaacaaa attgtgggta tgtacgccgc gccatccggc
      781 agtacgctgt tgatcgaccg caattgtcat aaatcgctgg cgcatctgtt gatgatgaac
      841 gatgtagtgc cagtctggct gaaaccgacg cgtaatgcgt tggggattct tggtgggatc
      901 ccgcgccgtg aatttactcg cgacagcatc gaagagaaag tcgctgccac cacgcaggca
      961 caatggccgg ttcatgcggt gattaccaac tccacctatg atggcttgct ctacaacacc
     1021 gactggatca aacagacgct ggatgtcccg tcgatccact tcgattccgc ctgggtgccg
     1081 tacacccatt ttcatccgat ctaccagggg aaaagtggta tgagcggcga gcgtgttgcg
     1141 ggaaaagtga tcttcgaaac gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct
     1201 tcgctgatcc acattaaagg cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg
     1261 cataccacca cctcgcccag ttatcccatt gttgcttcgg ttgagatggc ggcggcgatg
     1321 ctgcgtggta atccgggcaa acggctgatt aaccgttcgg tggagcgggc gctgcatttt
     1381 cgcaaagagg tccagcggtt gcgggaagag tctgacggtt ggtttttcga tatctggcaa
     1441 ccgccgcagg tggatgaagc cgaatgctgg cccgttgcgc caggcgaaca gtggcacggc
     1501 tttaacgatg cggatgccga tcatatgttt ctcgatccgg ttaaagtcac tattttgaca
     1561 ccggggatgg acgaacaggg caatatgagc gaggagggga tcccggcggc gctggtggca
     1621 aaattcctcg acgaacgtgg gatcgtagta gagaaaaccg gcccttataa cctgctgttt
     1681 ctctttagta ttggtatcga taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa
     1741 tttaaacgct cttacgatct caacctgcgg atcaaaaata tgctacccga tctctatgct
     1801 gaagatcccg atttctaccg caatatgcgt attcaggatc tggcgcaagg gatccataag
     1861 ctgattcgta aacacgatct tcccggtttg atgttgcggg cattcgatac tttgccggaa
     1921 atgatcatga cgccacatca ggcatggcag agacagatta aaggtgaagt agaaaccatt
     1981 gcgctggaac aactggtcgg tagagtatcg gcaaatatga tcctgcctta tccaccgggc
     2041 gtaccgctgt tgatgccagg agaaatgctg accaaagaga gccgcacggt actcgatttt
     2101 ctactgatgc tttgttccgt agggcaacat taccccggtt ttgaaacgga tattcacggc
     2161 gcgaaacagg acgaagacgg cgtttaccgc gtacgagtcc taaaaatggc gggataactt
     2221 gccagagcgg cttccgggcg agtaacgtgc tgttaacaaa taaaggagac gttatgctgg
     2281 gtttaaaaca ggttcaccat attgcgatta ttgcgacgga ttatgcggtg agcaaagctt
     2341 tctactgcga tattcttggt ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact
     2401 cgtggaaagg ggatttggcg cttaatgggc aatatgtgat tgagcttttc tcttttccgt
     2461 tcccgccgga gcgccccagc cgaccggaag cttgcggcct gcgtcatctg gcttttagcg
     2521 ttgatgatat cgatgcggca gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca
     2581 tccgtgtcga tccatacacg caaaaacgct tcacgttctt taacgatccg gacgggctgc
     2641 cgttggaact gtatgagcag taaggcttgt catcgccgca tttgcccggt aacgtgccgg
     2701 gcattgctac tgtaaaatcg caccatcatg acactcacgc tcaatagaca acttctcacc
     2761 tcacgccaga ttctggtggc ctttagcggc gggcttgact ccaccgttct gctgcatcag
     2821 ttggtgcagt ggcggacgga aaatccgggc gtcactctac gcgctatcca tgtgcatcat
     2881 ggtttaagtg ctaatgccga tgcctgggtt aggcattgcg agaacatctg ccaacagtgg
     2941 caggtgccgc tggtggtcga gcgcgtgcaa cttgcgcaag aaggactggg cattgaggcc
     3001 caggcgcggc aggcgcgtta tcaggcattt gctcgtaccc tgttacccgg tgaagtgctg
     3061 gtcaccgcgc aacatctcga cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt
     3121 agcggccctg ccgggctttc ggctatgggg gaagtttcgg aatttgccgg aacacggctt
     3181 atccgcccgt tgcttgcccg cacgcggggg gaactggagc agtgggcatt ggcgcatggt
     3241 ttacgctgga ttgaagacga aagtaatcag gacgactcat acgatcgtaa ctttctgcgc
     3301 ctgcgtgtag tgccgttatt gcagcagcgt tggccgcatt ttgccgaagc aacggcccgc
     3361 agcgccgcac tttgtgctga acaagagagt ctgttggatg aactgctggc ggatgattta
     3421 gcacactgcc aaacgtcgca ggggacgctg cagattgcgc caatgctggc gatgagtgat
     3481 gcccgtcgcg cggcgattat ccgccgctgg ctggcagggc agaatgcacc gatgccttcc
     3541 cgcgacgcgc tggtgcggat ctggcaggaa gtggcgctgg cgcgggaaga tgcctccccc
     3601 tgtttacgtt ttggcgcgtt tgaaatacgg cgctatcagt cgcaactgtg gtggattaaa
     3661 tccgtcactg ggcaaagcga aaccattgtg ccgtggcaga cgtggctaca accgctggaa
     3721 ttaccggcgg ggctgggaag tgtacagctt actgcgggag gcgatattcg ccctccgcgt
     3781 gcagacgaag cggtcagcgt gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgt
     3841 aacggcggac gtaagctaaa gaaaatctgg caagagctgg gcgtgccgcc gtggctacgt
     3901 gacaccacgc cactgctgtt ttatggcgaa acgctgattg cggcggcagg ggtatttgtg
     3961 acgcaagaag gtgtggctga aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt
     4021 agttaagtga aagccggata agacgcatca aacgtcgcat ccggcgaaag tcaatcagga
     4081 ttcgcttacc accaccgtac cgatttccgg gtggctaaag ctggtaattt tatccagacg
     4141 cagctcgcgg gtttcgccag cggcctcgac gaccagatac tccacatttt tgcgggagac
     4201 taaatcactg gcttttgcct gcaatttttc gccatctttc agctcaagtg tcagcattaa
     4261 atgatgctgg caggcgagct caagattatc gtaatcatca caattgattg gttgatacgc
     4321 atcattcatt gacataatcg ctcaccagta agtttgccgc agcgtatgct gctttttccc
     4381 tgacagcctc agaaagggcg tcgtcggcag ccatttcatt cagcactttc aaaacgcagc
     4441 ccagcgcgtc cggaacgtat cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta
     4501 ttaactcaca atatttttcc acatgccctc ctgtcagcac tctgacttaa ccgtggatgc
     4561 aagtctaagc ctacgaagat aaactctgtt tcgcaaggtg actataccac actcatttct
     4621 gcaatatcag cgccgcaact gcacgtattc cgttacaatg gcctcctgat tcgaaaggag
     4681 ttttcttatg gcgcttaaag cgacaattta taaagcgacg gttaatgtgg ccgatctcga
     4741 ccgcaaccag tttctcgatg cctctctgac gctggcgcgc cacccttcag aaacccagga
     4801 gcgtatgatg ctgcgcctgc tggcgtggct gaaatatgct gatgaacgtc tgcaatttac
     4861 ccgtggtttg tgtgccgatt atgagccgga agcgtggctg cgtaacgatc acctgggcat
     4921 tgatttgtgg attgagctgg ggctgccgga tgagcggcgg attaagaaag cctgcaccca
     4981 ggccgcaaaa gtggcgctgt ttacctataa tagccgggcg gcgcaaatct ggtggcagca
     5041 aaatcagagc aaatgtgtgc agtttgccaa tctttccgtc tggtatctgg acgatgaaca
     5101 actggcgaaa gtaagcgcct ttgccgatcg taccatgacg ctgcaggcaa cgattcagga
     5161 tggcgtgatc tggttatcgg atgataagaa taatctggaa gtgaacttaa ccgcctggca
     5221 acaaccttca tgattgtgat ttcccgacat gttgctattc ccgatggtga gcttgagatc
     5281 accgccattc gtgcgcaggg cgcgggcggg cagcatgtta ataagacctc aacggctatt
     5341 catctgcgtt ttgacattcg ggcgtccagc ctgccagagt attacaaaga gcgtctgctc
     5401 gccgccagcc atcatttgat cagcatgatg gcgtaattgt cattaaggca caggaatacc
     5461 gcagtcagga actgaaccgc gaagcggcgc tggcccggct ggtggcagtg attaaagatt
     5521 taacaacaga acaaaaagcc cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca
     5581 ggctggcata gaaagcacaa aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg
     5641 gtcgggaata aaaagaagga atggatggtg aaaaaagcga tagtgacagc gatggctgta
     5701 atcagcctct ttactctgat gggatgtaat aatcgggccg aagtcgatac gctttctccg
     5761 gcgcaggctg ccgaactaaa accgatgccg caaagttggc gcggcgtgct gccgtgtgcc
     5821 gattgcgaag ggatcgaaac ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat
     5881 gagcgttatc tcggtgctcg tgaagaacct tcctccttcg cttcctacgg tacatgggcg
     5941 cgaaccgctg acaagctggt attaaccgac agcaaaggtg aaaagtcata ttatcgggcg
     6001 aaaggcgatg cgctggagat gctcgatcgt gaaggcaatc caattgaatc gcagttcaac
     6061 tatacgctgg aaccggcaca atccagttta cctatgacgc cgatgaccct gcgaggcatg
     6121 tatttttata tggctgatgc ggcgaccttc actgattgcg cgaccggaaa acgtttcatg
     6181 gtagcgaata acgcagagct ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa
     6241 ccggtgttac tgtcagtaga aggtcacttt acgcttgagg ctaatccgga tactggtgcg
     6301 ccaaccaaag tattagcgcc cgatacggcg ggtaaatttt acccgaataa ggattgcagt
     6361 agtttggggc agtaaacctg cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc
     6421 gcttatcagg cctacagcat ataatgcaac attttgaatt agcacgattt tgtaggccgg
     6481 ataaggcgtt cacgacgcat ccggcatttt gtgcacgagg ctggctttat cactgagtca
     6541 gcccaacaaa gcgacccgcc ttgatataaa tccctggctt caggtgccca acatactgta
     6601 actgcgtttc tggcttaaac gcagagacat cgccagtacg catatgcatc agatcggcag
     6661 ggctaatcca gcctgattgc gccagcgtaa gcggatgccc ggctttggca aatgcatcag
     6721 tgacaaattc cgaacaaaac cacgactttt tgtctccttc gcccacactg cttaactgcg
     6781 ctttcgccag gccgctgacg cactgttggc gaaaatcctc ggagaacgga ttcagtgagc
     6841 acatctggcg agtcaccata aagggaataa attcgacaat gccgcgatag ttataactgc
     6901 tatctttgat tttattggca aacgcggtga tatctgtggc ttgttgcggg gtaagatccg
     6961 ggactcgtaa gacgaaaagc ttatcactat gcttcatcgc ttttttaagg gaaacgatct
     7021 ggacgccagc gcctgtcgct tctgcaacgt tattatcacc cagatagatt gcaacgtgac
     7081 tcacagagga agtgctgaag acgcggattc caaatgaggt taccccaagg cttgaggaga
     7141 acagcaaatc gccgggtttg agatcaggta ccgttatttc ttttattgat tgttcggtga
     7201 aagagctttg atgctggaat ttgacagccc atgtttttgc ctcggcatct actgctgtgg
     7261 ctgatgggtc tggctggctg atatcaacgg tacaagccga aagtaaaaga aagctgggga
     7321 gaagcaggcg gcagtacgcc tttggtttat ccattttata caatccatgt aaaaaagggc
     7381 cctgaaattc aggacccttt ctggcatcag cctttaatct gtttcaccag atattcgacg
     7441 atgtcaccag tcttaattaa ctgtttctcg ccgttgcgac gatatttata ttcgatatcg
     7501 tcgttgtcga ggttacggtc gcccagcaca atagtgtgcg gaataccgat cagttccata
     7561 tcagcaaaca tcacgcccgg acgctctttg cggtcatcca gcagcacttc gataccttgt
     7621 gcacgcagtt cgctgtacag tttctcagca agctcttgta cgcggaagga tttgtgcatg
     7681 ttcatcggca gaatcgccac ctggaacggc gcgatagcgt caggccatac gatgccgcgt
     7741 tcgtcgtagt tctgctcaat cgccgcagct accacacgcg ttaccccgat accgtagcaa
     7801 cccatcgtca ggatttggtt acggccatct tcaccctgta cggaggcttt cagtgcttcg
     7861 gagtacttgg tacccagctg gaagatgtga ccaacttcga taccacgttt gatcagcagc
     7921 gtaccctggc catccgggct tggatcgcca gccaccacgt tacggatatc tgcaacttcc
     7981 ggggtagcga catcgcgatc ccagttgata ccgaagtagt gtttaccatc gatgttagca
     8041 ccagcagcga aatcactcat cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg
     8101 tttaccggac ccagtgaacc cggaccggct ttaaccacag cacgaatttc ttcttcggtc
     8161 gcgaaagtca gcgggcttgc aacctgcggc agtttttctg ctttaacttc gttcagctcg
     8221 tgatcaccgc gcaccagcag cgcaaccagc gggaagctgc tgccttcaac cgctttaacc
     8281 agcagagtct taaccgtttt ctcaatcggc agattgaact gttcaaccag ttccgcgatg
     8341 gttttcgcgt tcggcgtatc aaccagcgtc atttcctggg tagcagcagc gcgcggttct
     8401 ttcggcgcga tagcttctgc cagttcaatg ttcgctgcat agtcagaggt gtcggagaag
     8461 accacatcgt cttcaccgct ctgcgccagc acctggaatt cgtgagaggc gctgccgccg
     8521 atagaaccgg tgtcggcttg cacggcgcgg aaatccagcc ccatgcggct gaagattttg
     8581 ctgtaggccg catacattgc atcgtaggtt tcctgcaggg attcctgaga agtatggaaa
     8641 gagtaagcat ctttcatcag gaattcgcgg gaacgcatga cgccgaaacg cggacgcact
     8701 tcgtcgcgga acttggtctg gatctgatag aagttcagcg gcagctgttt gtaagagcta
     8761 agctcgttac gaatcaggtc agtgataact tcttcatgag ttgggccgag tacgaacgga
     8821 cgctcgccac ggtcaacaaa acgcagcagt tccggaccgt actgttccca acgaccactc
     8881 tcttgccaca aatcggctgg ctgaaccacc ggcatcgaca cctcgattgc accggcgttg
     8941 ttcatctctt cacgcacgat gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc
     9001 caggtatata acccggaggc cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg
     9061 ctgatcacct cggcgtcggc aggtgtctcc ttgagagtgg agagcaggta ttggctagta
     9121 cgcatgttgt tacggttcca gttggaaggt agaacaggct caaggcgagc ctgggacaaa
     9181 aaaagtgatt tagtttacca gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac
     9241 gcggttccag cgcaaagact tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat
     9301 catgtagcca gacggcatag gttttgcccg tttcctcacc tttacgatag gccgggcgcg
     9361 ggtcctgcgc cagtacttcg cggataaaca gcgttaactg cggataacgc ttctccagcg
     9421 tcaaaagctg cttttcgacc tctgcggtaa aactcaccgc catctctgca gctggcgcac
     9481 tttgcgcata gctggcactg gcatcgggaa gcgattcggc aaaggggaga tacggtttga
     9541 tatccactac cggcgtacca tcgaccagat ccagactgcc gagcttcaga atcacgctgt
     9601 ctttatggca aacaacctct ttcagctcta ccagcgacat accaattggg ttagggcgga
     9661 aagtagagcg tgttgcgaaa acccccattc tggcgttacc gccgaggcgc ggtggacgca
     9721 cagtcggacg ccagccgcct tccatcgttt gatgaaagac gaaaaggatc cataaatggc
     9781 tgaacgcttc caggccgcga acggcgtcgg cctggttgta gggagcaatg agatgcagtt
     9841 cgccgttggc gctttttacc agaccaggct ggcgcggaac ggcgaatttt tctttatagg
     9901 gcgagcgaat aacgcctatt tgctcgaact ggaaactgct catttcgccg taatgttaag
     9961 cgcagagccg atacatacag cctgacgata gcagcctagc gtaccgctgg tgacttcgca
    10021 gctatgcagt aataccgcat tggctttcat tttagaggcg ttgatttgca tccgcttacg
    10081 cgcggttgga atgctcggcg gagagtcctg attagaggcc tggcaagagt cgccactgac
    10141 ttcaccgaga tcgcggaacg gtttgccgac taattcttct gcattggtat aaattcggac
    10201 cggcgtggcg cgcggcgctt tcggttttgc aggctccgct ttcggctggg gtgcagtgct
    10261 ttgaacgggt tcgacagggg atctgcttaa catggaacag ccgcttagca tgagtgctac
    10321 taaacagatc ggtaaagcac gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt
    10381 gaatcaggag cttgtaaaaa tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa
    10441 attgtggaaa cagcctaaaa attaccagcc tttaacagcg ccgccgttaa acactttgtt
    10501 tgctgcttcg taaacttcat cagactgata agcctggacg aatttcttca cgttctcggc
    10561 gtctttgtta tcttcacgcg ttacgatcag gttcacgtac ggggagtctt tatcttcgac
    10621 aaagataccg tctttcgctg gagtcaggcc aatctggctg gcataggtgg tattgataac
    10681 tgccagagcg atttgcgcgt cgtccagaga tcgaggcagc tgcggtgctt ccagctcaac
    10741 aattttcaga ttttttgggt tctcaacaac atcaagaacg gtcggcagca ggccaacgcc
    10801 atctttcagt ttgatcaagc ccactttttg cagcagcagc agtgaacgac caagattagt
    10861 tggatcgttt ggcacggcaa cctgcgaacc atcctgcagt tcatccagtg atttgatttt
    10921 cttggagtaa ccagcaatcg gataaacaaa agtgttgcct actgcgacca gtttgtagcc
    10981 acgatctttc agttgctgat caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc
    11041 gcctttgctc aatgcttcgt ttggcagaac atagtcgttg aaggttacca gctcaacgtc
    11101 caggccatat ttgtctttcg caactttctg cgcgacttct gctacctgct gttcggcacc
    11161 aacaatcacg ccgactttaa tgtggtttgg atctttttca tcctgaccgc agcctaccag
    11221 tgccagtgat ccaatcaggg ctcccactgc cgcaaaggtt ttgaatttga acgccatacc
    11281 ttattccttc ttcaattatt tatgttgtgt tgaacgttac ttgcgagtga cagcccggac
    11341 gatgcggtcg cctgcgaact gaattaaata aaccagaatg accagcaata ccagtaccgt
    11401 attcatcacc gtcgcgttgt agccgatgta gccatactga tagccaatct gacctaaacc
    11461 accggcaccg actgcaccac ccatcgcgga ataaccgacc agggtaatca gggtgatagt
    11521 tgccgcattc accagacccg gcaacgcttc cggtaacagc actttacgga cgatctgcat
    11581 tggcgtggca cccattgcgc gggaagcttc aattaacccg gttgggatct ccagcagagc
    11641 gttctcgacc atacgggcaa taaacggtgc tgcaccaacg gttaacggaa caattgctgc
    11701 ctgcaaacca atcgatgtac cgacaataac gcgggtaaac ggaatcatcc ataccagcaa
    11761 gataatgaac gggatggaac ggaaaatgtt cacaatcgca gaaacggtac gatacagctt
    11821 cgcgttagca ataatttgcc ccggacgcgt gacataaagc agaacgccaa ccggcagacc
    11881 aatcacaaag ccaaaaaatc cggatacgaa ggtcattgcc agcgtttccc atacgccacg
    11941 aaccagcagc cacatcatcg gctcagacat aacccagtac ctctactttt acatggtgtt
    12001 cctgcagcca ggcaatggcg gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca
    12061 tgatgccgaa tttaacgcca ccggcgtagt ccatctgcgc gctaataatg ttgttgttga
    12121 cgttgaaacg acgcgcggtt tcagaaagca gtggggcatc gaccgattga ccggtaaact
    12181 ccagacgcag catcggcacg cagtcagtaa atggctccgc ttgcagacgt tcctggtaat
    12241 cttccgggat atccagatgc agggtcgact gaataaactt ctgcgccagc ggcgttttcg
    12301 gatgcgagaa cacttcactt accgtgtcct gctcgatcag ttcgccattg ctgatgaccg
    12361 ccacgcaatc acaaatacgc ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca
    12421 aacccagacg gcggttaatg tctttcagca gttcgagaat agaacgtgtc gttgccgggt
    12481 ccagcgcgct ggtggcttca tcacacagca atactttggg attgctggct aatgcacggg
    12541 caatcgccac acgttgtttc tgcccaccgg aaagattcga cgggtagcta tcatgcttat
    12601 cgccaagacc aaccaatgac agcaattccg tcacgcgacg tttgatctcg tctttcggtg
    12661 tgttgtccag ctccagcggc agagccacgt tgccaaaaac agtacgcgaa gagagcaggt
    12721 taaaatgctg gaaaatcata ccaatctggc ggcgagcttt ggtcaactcg gattctgaca
    12781 gcgtggtcaa ttcttggcca tcgaccagca cgctaccctc ggttgggcgc tccagcaggt
    12841 ttacacaacg tataagcgta ctcttacccg cgcctgaggc accgataacg ccataaattt
    12901 gtccagctgg cacatgcagg ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt
    12961 ggaacacttt ggtgatattc gaaagtttta tcattgatta tttattatcg tcattaagtt
    13021 agtcgtggca tctcgaatgc ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc
    13081 atccagacgt ctaaatcaat caggtttatg cgaagagcac tttcttgcag gtcgaaacat
    13141 gcgatactag cgtcccatgc cttattaagg agctataaaa ggtggcgaag agcgtacccg
    13201 caatttttct tgaccgtgat ggcaccatta atgtcgatca cggctatgtc catgagatcg
    13261 acaactttga atttatcgac ggtgttattg acgccatgcg cgagctaaaa aaaatgggct
    13321 ttgcgctggt ggtagtaacc aaccagtctg gcattgctcg cggtaaattt accgaagcac
    13381 agtttgaaac gctgaccgag tggatggact ggtcgctggc ggaccgagat gtcgatctgg
    13441 atggtatcta ttattgcccg catcatccgc agggtagtgt tgaagagttt cgccaggtct
    13501 gcgattgccg caaaccacat ccggggatgc ttttgtcagc acgcgattat ttgcatattg
    13561 atatggccgc ttcttatatg gtgggcgata aattagaaga tatgcaggca gcggttgcgg
    13621 cgaacgtggg aacaaaagtg ctggtgcgta cgggtaaacc tattacacct gaagcagaaa
    13681 acgcggcaga ttgggtgtta aatagcctgg cagacctgcc gcaagcgata aaaaagcagc
    13741 aaaaaccggc gcaatgatta aaagatgagc ggttgaaata aaaatgcatt tttccgcttg
    13801 tcttcctgag ccgactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc
    13861 acacaaacag ccggttcggt tgaagagaaa aaatcctgaa attcagggtt gactctgaaa
    13921 gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagctgcgt cgcaactgct
    13981 ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc
    14041 aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac ga
//
LOCUS       DAJTVK010000108        13903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000108 DAJTVK010000000
VERSION     DAJTVK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.108"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="OO372_003209"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="OO372_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6342623.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="OO372_003210"
     CDS             complement(1550..1723)
                     /locus_tag="OO372_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6342624.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="OO372_003211"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="OO372_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6342625.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="OO372_003212"
     CDS             3361..3690
                     /locus_tag="OO372_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6342626.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OO372_003213"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="OO372_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6342627.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OO372_003214"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="OO372_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6342628.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OO372_003215"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="OO372_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6342629.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OO372_003216"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="OO372_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6342630.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OO372_003217"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="OO372_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6342631.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OO372_003218"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="OO372_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6342632.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="OO372_003219"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="OO372_003219"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6342633.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3658 a         3437 c         3230 g         3578 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcggtcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTVK010000109        13794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000109 DAJTVK010000000
VERSION     DAJTVK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.109"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..147)
                     /locus_tag="OO372_003220"
     CDS             complement(<1..147)
                     /locus_tag="OO372_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6342634.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="OO372_003221"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="OO372_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="OO372_003222"
                     /pseudo
     CDS             476..1021
                     /locus_tag="OO372_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OO372_003223"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="OO372_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6342635.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="OO372_003224"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="OO372_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6342636.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="OO372_003225"
     CDS             3028..3201
                     /locus_tag="OO372_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342637.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OO372_003226"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="OO372_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR6342638.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="OO372_003227"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="OO372_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6342639.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="OO372_003228"
     CDS             complement(5499..6422)
                     /locus_tag="OO372_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6342640.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="OO372_003229"
     CDS             6639..7982
                     /locus_tag="OO372_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6342641.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="OO372_003230"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="OO372_003230"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6342642.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="OO372_003231"
     CDS             10002..10226
                     /locus_tag="OO372_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6342643.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="OO372_003232"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="OO372_003232"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6342644.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /locus_tag="OO372_003233"
     CDS             complement(10820..11800)
                     /locus_tag="OO372_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCR6342645.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="OO372_003234"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="OO372_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6342646.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="OO372_003235"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="OO372_003235"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6342647.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3482 g         3575 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gctcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAJTVK010000110        13778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000110 DAJTVK010000000
VERSION     DAJTVK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.110"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OO372_003236"
     CDS             complement(<1..730)
                     /gene="dsbG"
                     /locus_tag="OO372_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6342648.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNI"
     gene            1102..1665
                     /gene="ahpC"
                     /locus_tag="OO372_003237"
     CDS             1102..1665
                     /gene="ahpC"
                     /locus_tag="OO372_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6342649.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1836..3401
                     /gene="ahpF"
                     /locus_tag="OO372_003238"
     CDS             1836..3401
                     /gene="ahpF"
                     /locus_tag="OO372_003238"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6342650.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OO372_003239"
     CDS             complement(3522..3950)
                     /gene="uspG"
                     /locus_tag="OO372_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6342651.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4171..5409
                     /locus_tag="OO372_003240"
     CDS             4171..5409
                     /locus_tag="OO372_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR6342652.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OO372_003241"
     CDS             complement(5640..6050)
                     /gene="rnk"
                     /locus_tag="OO372_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6342653.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OO372_003242"
     CDS             complement(6280..7086)
                     /gene="rna"
                     /locus_tag="OO372_003242"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6342654.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OO372_003243"
     CDS             complement(7200..8663)
                     /gene="citT"
                     /locus_tag="OO372_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR6342655.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OO372_003244"
     CDS             complement(8714..9592)
                     /gene="citG"
                     /locus_tag="OO372_003244"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6342656.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OO372_003245"
     CDS             complement(9567..10118)
                     /gene="citX"
                     /locus_tag="OO372_003245"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCR6342657.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OO372_003246"
     CDS             complement(10122..11654)
                     /gene="citF"
                     /locus_tag="OO372_003246"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6342658.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OO372_003247"
     CDS             complement(11665..12573)
                     /gene="citE"
                     /locus_tag="OO372_003247"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6342659.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OO372_003248"
     CDS             complement(12570..12866)
                     /gene="citD"
                     /locus_tag="OO372_003248"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6342660.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12881..>13778)
                     /gene="citC"
                     /locus_tag="OO372_003249"
     CDS             complement(12881..>13778)
                     /gene="citC"
                     /locus_tag="OO372_003249"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6342661.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3379 a         3490 c         3670 g         3239 t
ORIGIN      
        1 tgatattaag cgttttctga tcgggcaacc ccacggcctg ttgcagcgta ttttccttac
       61 tcatgtaata gatagccggc gtgacatttg cccccagatc gtccatcagt ttctcattgt
      121 cacttaacac tttcatttgc tctgtactta cgtttgcagg cacgtttagc ttaagcttgc
      181 caccagaggc ttcatattgt tgccaggttt ttgcgggatc tttggaggca agaattgccg
      241 ctgctgtcgc cgggctttct ggcttgataa ccccaaccaa caatgttctt aattgcactt
      301 tgccagaatc tacccacggg cgcgcctgct gccagaactg tttacaatat gggcagaacg
      361 gatcggcgaa gacgtagaca atgaccggcg catctttttt accgtcgagg atccagtggg
      421 attgttccat ccgttgccac atttcgcgtc cggctggtgc gtaaatttct ttttcgataa
      481 gtgtgttact caggttttca cctttctcgt tgtacatgta accagagata gcgtgcttac
      541 catcgggagt caggtagatg gtgacgccca tatcctgata ctttccgaga taacctttca
      601 ttcctccggg ggcatcgaac gttttgatga ttgtaatgcc ctgtttttca atcgctttta
      661 ctggggaagg aagttcctct gcgaaggcga ttgcaggaag cagagccagt aaaagtatct
      721 tttttaacat taatttgtcc ttttcagtca gtgcaaaagt cgagtaaaag gcataaccta
      781 tcactgtcat aggtaagagc ttagatcagg tgattgccct ttgtttatga gggtgttgta
      841 atccatgtcg ttgttgcatt tgtaagggca acacctcagc ctgcaggcag gcgctgaaga
      901 taccaaaggg tagttcagat tacacggtca cctggaaagg gggccatttt actttttatc
      961 gccgctggcg gtgcaaagtt cacaaagttg tcttacgaag gttgtaaggt aaaacttatc
     1021 gatttgataa tggaaacgca ttagccgaat cggcaaaaat tggttacctt acatctcatc
     1081 gaaaacacgg aggaagtata gatgtccttg attaacacca aaattaaacc ttttaaaaac
     1141 caggcattca aaaacggcga attcatcgaa atcaccgaaa aagataccga aggccgctgg
     1201 agcgtcttct tcttctaccc ggctgacttt actttcgtat gcccgaccga actgggtgac
     1261 gttgctgacc actacgaaga actgcagaaa ctgggcgtag acgtatacgc agtatctacc
     1321 gatactcact tcacccacaa agcatggcac agcagctctg aaaccatcgc taaaatcaaa
     1381 tatgcgatga tcggcgaccc gactggcgcc ctgacccgta acttcgacaa catgcgtgaa
     1441 gatgaaggtc tggctgaccg tgcgaccttc gttgttgacc cgcagggtat catccaggca
     1501 atcgaagtta ccgctgaagg cattggccgt gacgcgtctg atctgctgcg taaaatcaaa
     1561 gcagctcagt acgtagcttc tcacccaggt gaagtttgcc cggctaaatg gaaagaaggt
     1621 gaagcaactc tggctccgtc tctggacctg gttggtaaaa tctaaatttc cttcgtcttt
     1681 cgcgctacca gctccggcgc gaaatacttc ggaaattcac ctaattcttc gggtgctgcg
     1741 gcacccgatt ttcttccccg caccatgatg caagctgcat ccaggtagcc gtagaggccg
     1801 cttgcatgat gatgtttaaa gcccaggaga taaacatgct cgacacaaat atgaaaactc
     1861 aactcaaggc ttaccttgag aaattgacca agcctgttga gttaattgcc acgctggatg
     1921 acagcgctaa atcggcagaa atcaaggaac tgttggctga aatcgcagaa ctgtcagaca
     1981 aagtcacctt taaagaagat aacagcttgc cggtgcgtaa gccgtctttc ctgatcacca
     2041 acccaggttc caaccagggg ccacgttttg caggctcccc gctgggccac gagttcacct
     2101 cgctggtact ggcgttgctg tggaccggtg gtcatccgtc gaaagaagcg cagtctctgc
     2161 tggagcagat tcgccatatt gacggtgatt ttgaattcga aacctattac tcgctctctt
     2221 gccataactg cccggacgtg gtgcaggcgc tgaacctgat gagcgtactg aacccgcgca
     2281 tcaagcacac tgcaattgac ggcggcacct tccagaacga aatcaccgat cgcaacgtga
     2341 tgggcgttcc ggcagtgttc gtaaacggga aagagtttgg tcagggccgc atgacgttga
     2401 ctgaaatcgt tgccaaaatt gatacgggcg cggaaaaacg tgcggcagaa gagctgaaca
     2461 agcgtgatgc ttatgacgta ttaatcgtcg gttccggccc ggcgggtgca gcggcagcaa
     2521 tttactccgc acgtaaaggc atccgtaccg gtctgatggg cgaacgtttt ggtggtcaga
     2581 tcctcgatac cgttgatatc gaaaactaca tttctgtacc gaagactgaa gggcagaagc
     2641 tggcaggcgc actgaaagtt cacgttgatg aatacgacgt tgatgtgatc gacagccaga
     2701 gcgccagcaa actgatccca gcagcagttg aaggtggtct gcatcagatt gaaacagctt
     2761 ctggcgcggt actgaaagca cgcagcatta tcgtggcgac cggtgcaaaa tggcgcaaca
     2821 tgaacgttcc gggcgaagat cagtatcgca ccaaaggcgt gacctactgc ccgcactgcg
     2881 acggcccgct gtttaaaggt aaacgcgtag cggttatcgg cggcggtaac tccggcgtgg
     2941 aagcggcaat tgacctggcg ggtatcgttg agcacgtaac gctgctggaa tttgcgccag
     3001 aaatgaaagc cgaccaggtt ctgcaggaca aactgcgcag cctgaaaaac gtcgacatta
     3061 ttctgaatgc gcaaaccacg gaagtgaaag gcgacggcag caaagtcgtt ggtctggaat
     3121 atcgagatcg tgtcagcggc gatattcaca acatcgaact ggccggtatt ttcgtgcaga
     3181 ttggtctgct gccgaacacc aactggctcg aaggcgcagt cgaacgtaac cgcatgggtg
     3241 agattatcat tgatgcgaaa tgcgaaacca acgtcaaagg cgtattcgca gcgggtgact
     3301 gtacgacggt tccgtacaag cagatcatca tcgccactgg cgaaggtgcc aaagcctctt
     3361 tgagtgcttt tgactacctg attcgcacca aaactgcata agaagaagta agattcacct
     3421 gcaattgctt agccgccggg gtcaaacctg gcggcttttt tatggcatta aaaagccctg
     3481 ccgggatgtt ccaggcaggg cggcataaat acttttatct gttagcgaat gaccagtaca
     3541 ggcagattgg cgtggcggat cacgctcgag gcgttagaac ctaacagatg ggtcgaaatc
     3601 gatgggttgc gagaaccgat aactacaaca tcagccccca gttcttctgc caactcattg
     3661 acttcatccc gcacgctacc aaaacggaca tgttgtttaa tgcgggaagg atcgatggtg
     3721 aagtggctga ccatcgtttg cagacgttct tctgcttcat gttgcagatg ctcttcaaaa
     3781 cgacgcacat cagcggcaaa acggtgcagg ctcaggctgg ctgacccggg tagtacgtga
     3841 agtagatgaa taactccgtc atcctgggcg aggaattcag cgtggcgaac agctttgtcg
     3901 ctcaattcca tttcaaatac atcaactggc ataatgattg tcttatacat aaccctttct
     3961 ccctgttaat catgaacaaa tcattcgcca tgattataat atttatccct gatatttgtc
     4021 tggttctttt tccttacgaa cggtttctgt gattaatata ttctcactga acaccaggaa
     4081 ttctcccaaa acctgcggta ccgcccgttt tcccgctgtg atagctaccc ttaaagactg
     4141 actctttttt gaactgtctc tggaggttgc atgaaagcat tgacttatca cggcccacat
     4201 cacgttcagg tagaaaatgt tcccgatccg ggcattgaac aggctgatga tattattctg
     4261 cgtattacgg caacggcgat ctgtggctct gacctccatc tttatcgagg caaaataccc
     4321 caggttaaac atggcgatat tttgggtcat gaatttatgg gggaagtcgt tgaaaccgga
     4381 aaggacgtaa aaaatttgca aaaaggcgac cgggtggtaa ttccgtttgt cattgcttgt
     4441 ggcgactgtt ttttctgtcg aatgcagcaa tatgccgcct gcgaaaatac caatgcgggt
     4501 aaaggcgctg cgctcaataa aaaacagata ccagctcccg cggcattgtt tggttatagt
     4561 cacctgtatg gtggcgttcc tggtggacag gcggaatatg ttcgcgtccc taaagggaat
     4621 gtggggccgt ttaaagtacc gcctttgctt tcagatgata aagccctttt cctttctgat
     4681 attctgccaa cggcatggca ggcagcaaaa aatgcgcaga tccaacaagg ttcaagcgtt
     4741 gcagtctatg gtgccggtcc agtgggattg ttgacaatcg cctgtgcacg gttgctcggt
     4801 gcggaacaga tttttgttgt tgatcatcat ccctaccgct tgcgtttcgc cgctgaccgc
     4861 tacggcgcga tcccgattaa tttcgatgaa gatagcgatc cggcacagtc aattattgaa
     4921 caaacggcag gtcaccgggg cgtggatgca gtaatagacg ccgtcggttt tgaagcgaaa
     4981 ggcagcacca cggaaacggt gctgactaac ctgaaactgg agggcagcag cggtaaagcg
     5041 ttgcgccagt gtattgcggc ggtcaggcgt ggcggcattg ttagcgtacc gggcgtctac
     5101 gctggattta ttcacggttt cctgtttggc gacgcctttg ataaagggtt gacgtttaaa
     5161 atgggacaga cccacgttca cgcatggctg ggtgaattat taccgttaat tgagaaagga
     5221 ttactgaaac cagaagaaat tgttacccac tatatgccgt ttgaagaggc cgcccgggga
     5281 tatgagattt tcgaaaaacg tgaagaggag tgccgtaagg tgattctggt acccggtgca
     5341 caaagcgcag aggcggcgca gaaggcggtt tcaggtctgg tgaatgcgat gccgggggga
     5401 acaatatgat cgtcaggagt ggttttcgag gtaaaggaca gccatgacga taatcgccgc
     5461 cataatcaga aatcctatca ggatgtaaaa tgcttctgcc atggttattc ccacaaacga
     5521 aacggaataa ttttgcagca aaagtgaaca gtgagaacca ggaaaaattg ctgattttgc
     5581 gtaaagagga tgcgagtgca tcctctgggc aaagcgagtt atcgcttgtg cagatgggat
     5641 taaagcaggt agtcgccagc agcttctggc tggtattcga gttccagcac ttcaaggtgg
     5701 gttgcaacgc cgcccggaag ttcccagtga atggaatcgc caacgcgcag ccccagcagt
     5761 gcggcaccta ccggagccat aacggaaagc tgagtattgc tatcggtcat ttttgccgga
     5821 tacaccagcg tgcgcacacg cacttcgcca tcgctaagat tgcggaattt aacccggctg
     5881 ttcattgtca ccacgtcgtg tggcatctct tctggcgaac acatttgggc gcgatccaac
     5941 tctgcgttta acgcgtcggc gattggcaaa ccagcatagg cgggttgctc cagcagaata
     6001 tcgatgcgtt cggcatccag gtcgttaatg atgatagttg gtctggacat ttttactcca
     6061 tgtcgtcggt actgcgagtg tcgcagataa acatacccaa aagaaaaccc tcaccgtcag
     6121 gcggcgaggg tttaactcac atgatgatac tgactgttgc tcactctttg aagtgatttg
     6181 cgtcacattc agggaattcc tcaatgcagc gcattatgta taattcttaa tcgtcacggt
     6241 ttatggaaga cgaattagcg tgttttgtaa atcagatgat taataaccca ctttatcaat
     6301 cacaaagttt ttgccacagt tgcctgggtg tggttgtggc aagaatgagt ttgcagaaag
     6361 cggagcgttg atggcgtcag ctttgatcga gatctgaatt tcagtcaaat acgcagggtt
     6421 accctggcac gttagcttaa ctgctttcac gttctctttt ccccagcttt tggcaaaggc
     6481 ggcgtcaaag tcacggcggc tcactgtttt accgtaatta tccgcaagga attttccggc
     6541 ttcgctctct ttaatttctt gattcaggcg taccatcgta ccgaagtatg cgtccggatc
     6601 aaaaccaaag caggcaccgt gtttggcata ttcgtagcgt tccaggcagg aacgtccgcc
     6661 agctcctggc atgacttcac ttagtttagc ggccgtttcc agtgataacc cggtttccgg
     6721 cgatgaacac atccggctgg cgtgcgcttc tggtagattc gggattgggc gagtagcgca
     6781 accgaagcgc atccagcggc gttcatcaac accacgggca gcaaccgatt taggcaatcc
     6841 tggccacaga ccatgtacgg tcagaaaatc agctttgttg gtcgtttcgg tttgtaggcg
     6901 acattcatct cgttcgttac gatttcgatc gtgttgactc tggcaaaatc cggtttgcca
     6961 ggagagggcc aggacatagc gatcaaaatc gccatactgt tttgcctgca acgctacggc
     7021 gttggcagaa gagaagggaa gcagagaaac cgcgagcaac gcggcgttac gccagaatgc
     7081 tttcataatg ggtgtggaac tcatacatac actgaatact atctattaaa tcataaaaaa
     7141 gcccgccagg tgggcttacc tggcgggcgt gatgatttat tcagcgtttg gcgaacgtat
     7201 tagttccaca tggcgagaat cggccagcca accaacagca acatagagat gtaaatcacc
     7261 ccgaagattg cgccaagacg ccagtaatct tttgatttca catagccaca gccgtaaata
     7321 atcaccccag gaccggttgc atacggcgtc agacagccca tgataccgat agacagcacc
     7381 agcaggatac acagttgttc cattggtacg cccggaatac ctttaccgac ggccagaata
     7441 accgacagca tggttgcggt gtgcgcagac aggctggcaa acaggtagtg tgcaaagtag
     7501 aacaccagaa ccagtacaat caccgttgcg tttggtgaga atccttccag gtgcgtactc
     7561 atggtaccgg cgaaccagtc aataaagcca gaacgagtca ggccgttagc catcacaacc
     7621 agagttgcca ggttgaccag cgtgttccat gcgctgttat agcgggtaat atctttccaa
     7681 ggcacaacgt gcagggccag cattagcgaa actgccagca gaccaactgc agtagcatta
     7741 atgacttcac tgccaaaaac ccacaaacct aagctgagca atacaaggcc aatcagtgtc
     7801 cactctctgc gtgtcagcgc acccatggtt ttcagttcat cacccgccca ggttgccact
     7861 tcttcactgt gtgtgatttc cggtttgtac agcacgtagg aaagccacgg cgcaatgata
     7921 agcaagataa ccccaaccgg caggaagcag aggaaccact gcaaccagct aatctggata
     7981 ccggcaattt tgctgacgaa ctccagaccc agcacgtttg gtgccgcacc ggtgacaaac
     8041 atggacgaac tcagactggt actaatgacc atcatccaca tcaaatagcc gccaatacga
     8101 cgcgcggacg gatcgttggg gaatgattta aacagcggcg gcaggttttt aatgaccgga
     8161 aaaaccgtac ccccggtacg cgcggtgttg gacggtgtaa acggtgccag cagaatgtcg
     8221 ataatgacaa tcgcataacc caacgtcagc gtgcgtttgc ccatgaattt caccaggaaa
     8281 agggcaatgc gacgacctaa cccggaaact tcatacccta atgcaaaaat aaatgcgcca
     8341 aataccagcc ataccgtggt gctggaaaaa ccagccagac cccatttcag cgcctgtttt
     8401 tgcgcattaa acgctgggtc agctaattct ttggcatcaa agagcaggta attactgcca
     8461 ataacgcaaa tagtaaccgc aataaaactg atcgctgttg ccggaattgg ctcgaggatc
     8521 atgccgacaa tcattgccac aaacacagcg aagtaatgcc atgcctgtgg tggcataccg
     8581 tcggggacag ggataagaaa catgacaccc atcaccacca gtggggccaa tagtttccat
     8641 atattatctt ttgctaaaga catacgggtt ctccgaaaat taatatttcc aaatttatca
     8701 agtgcttaaa taattaaatc tgtgctaaaa accaggtaag gatcagcagg tcggcactgc
     8761 cgcctggact gagatttcgt tcgatacact ccctgtcgaa ctgccggaga taatcgagat
     8821 cggcgggggt tcgaatgccc cctttttgca ataatgtttg cgcctcgcgc tgtagccagc
     8881 gcaggccccc ctcgccaccg cgcgatgcaa cgttggtatc gccgttgatc gccatcagta
     8941 ggagcaaggt atcgagcaat gccagttcag gatctaaccc ctgatccagc agagtgaggt
     9001 aatgcggcaa ggcgtgattg atcaccagtg gataacccgc ttcggcttca ccgcgtgcgc
     9061 cggtaaggcc aagctgctgg tacaaccgtt gacctgccgt cagttgtgaa ttattggtac
     9121 gcagttctcg atcggtcagg ccacggcaga aacttgccgc cgtagaacaa acggttgttg
     9181 gcgttaccgg ttggttgagt tgaagcaaac ggccaattgc cgcacatagt agccctaaag
     9241 aaaaaatgct gcctttatgc gtgtttacgc ccgcagtggc gcggaacata tcaccttcgc
     9301 aagccatacc aattgggcgt aatccgtgga gtaccgcttc tggtgctatt tccgcactac
     9361 aggcaccaaa ttcaatgaaa cggggtagcc agccctgaat cgccagcgcg ctgcggtgga
     9421 aatcttccag cgccatatct ttatgtgcac cgcagttaat ccgatccacg aggcctggtt
     9481 tcggtgacag attgacttca gtcagcatgg cgcgccagcc cagcagggcg tactcatcga
     9541 tcaatgacgt cgcaagcttt gtggttttag ttgacgttgc aggcatcgac atcgttcagc
     9601 agtgcctcca tgcggttgag taaatcggtc agttgatggg tttttccacg cgcgcagacg
     9661 gctgcgcttt gctcgcacaa caggcagcgg cgaggcggca gtgaatagtc gcggcgggag
     9721 agaatttcgc cttcgggcgt caggacatcg atatcccata accgcccgag aggatgacta
     9781 tgttcaagct caatggtggc gagcttgagg tcgcgagccg gggcggcaat gctcaacatg
     9841 ccctccggcc cgctggcgga aaccagtgca gcctgctcct gaatttgcca gccctgtttt
     9901 gcggctaagg cacgcaaggc tgtcacgcca tgattaaaaa ttcggcgtgt gacctcgctg
     9961 tctttaatcg gcccaggcgc aaccacggta aaggagacca gtggaacagg atggcgcttg
    10021 agccagacgt gttgccgtgc ttgcctttca tcccggctga cgagcagctc gggaattgac
    10081 accgcatggt ggctggcgag ttcaggaagc aggtgcatgg cttattcctt cacctgatgc
    10141 acaacatcga tcaccgagcc atcgcggtaa cgcacaacgg caacgacgcg gtcggtgaat
    10201 tcaatcggct gtggttcacc ggtcagcaga cgcgcgcgtt cgcgcagcca ctcaatggaa
    10261 accactttaa tgcccgcttc ctgcagacgt tctgccagtt ccggacgtgc cgggttaact
    10321 gcgataccgt ggtctgtgac cagaatatcg acactggagc ctggggtgat gcaggtcagc
    10381 acgttatcca ccagagtcgg aatacgaccg cgtaccagcg gcgcgacgat gatggaaagc
    10441 gcagaggcaa tcgcggtatc gcagtgacca ccggaagcac cacgcagtac gccgtcagag
    10501 ccggtcagca cgttaacgtt gaactgggtg tcaatttcca gcgcgctcag taccaccacg
    10561 tcgagacgat caaccgatgc gcctttcgaa ccccagttag cgtactggtt ggcgctgatt
    10621 tcgatgtgat tggggttacg ggccagcgat tgcgcagcat ggctgtcaaa gctctgcaca
    10681 tccagcagtt tgcggatcag acctttttcg tgcagatcaa ccatcgtcgc ggtaataccg
    10741 ccgagggcga agtcggcgcg aatatcgcgg ctacgcattt tgtcttccag gaaacgggtt
    10801 accgccagcg atgcgccgcc ggtgccggtt tgcatggaga aaccttcttt gaagtagcca
    10861 gagttgacaa tcacatccgc agcgctacgg gcaataagca gttcgcgcgg gttagtggtc
    10921 atacgggtcg cgccagcgcc gatttttgca gcatcgccaa cgcggtcaac tttgacgatc
    10981 aaatcaacct gatcttgctc aatgcttgcc ggattatgcg gataaggcag cagttcttcg
    11041 gtaagcatca caacctgttt tgcgttgtcg gcatcaacca ttgcatagcc gagggagccg
    11101 cagcaggctt taccggtgta accgttggca ttaccgaatt catcacagga cgggacgccg
    11161 aggaaagcca cgtcgatatt cagttcgccg ctctgtacca gatgcacacg tccgccgtga
    11221 gagtggattt gcaccggttc cgcaagcaga ccacgggaaa tttcttctgc cagtggaccg
    11281 cgcaggccgg aggtataaat gcgggtaacc acgccctggc gaatgtgttc taccagtggc
    11341 gcatggcaat cactcaggga gctggacgcc agggtcaggt ttttaaagcc catcttagcg
    11401 atgacgtcca tcaccatatt gacggtcagg tcaccgccac ggaaagcgtg atggaaggaa
    11461 accgtcatgc cgtcctgtaa accagagcga cgaatcgctt cttcctggtt ggcgcacagt
    11521 ttgcgatcgc gcgctttttc agcctggtag gtttgctttg gtgagttctg gaaagcggca
    11581 agatcgcatt cagcgcgacg attccaggcc gctacccgtt cttgtcgttg agattgttca
    11641 attttctgcg tcattttgat tgccttattc ttcgcggatg ccggaaagtt ctgcacggga
    11701 gagcaccaga cgggcacgat cgataaccgg accgtccacc atcttgccgt tcagggaaac
    11761 cacgccgagg ccttcgcgag cggcggcttc agcggcttct acgacgcggc gggcgtgatc
    11821 cacttctttc tgggtcggtg cgtagaggtt gtgcaacaga tcaatctgac gcgggttgat
    11881 cagcgatttg ccgtcaaagc ccagctgttt gatgtgggcg gcttcttgca ggaatccggc
    11941 ttcgttgtta gcgtcggaat agacggtatc gaacgcctga atacccgcag agcgcgcggc
    12001 ctgcaaaatg gaacagcgtg cgaacagcag ttcagttcct tccggggagc gttctgtacg
    12061 caggttgcgc acatagtctt ctgcaccgag ggcgataccg atcaaacgct cggaagcgtg
    12121 agcgatttcc actgcgcggg taatccccag cggagattca atcgccgcca gcaggccggt
    12181 gctgccgggt tcacgaccac aggctttttc gatacgcagg atctcttttt cgatatccag
    12241 aacatcctga gcggtatcgg ttttcggcag acgcacaacg tccgcaccac cgcgaacgac
    12301 ggcttccagg tcgttgacac cccattcgga atccagcgcg ttgacacgca caatggtttc
    12361 aatatcgcga tacagcggat gttgcagcgc gtggtaaacc atgcggcggg cggtgtcttt
    12421 ttcacgcaat gctacggagt cttcgaggtc aaacatcagg gcatcagccg ggtagatgaa
    12481 ggagttgctg accatcgcgg cattggcacc aggcacaaac aacatgctgc ggcgggtgcg
    12541 agttttacgt tgttgcagcg aagcggaaat cattggcaat cctcccatgg cagagccggg
    12601 ataccgctgg cgcgtgccag cagggcttcc agtcgtgcac gtaaaatgca gtccagtgcg
    12661 cctttgtcat cgacattcag ctgtacgccg cgcacgttgt agcgggcgag aacgtccaga
    12721 atggtggtgc gaattgcatc gccaaactgt ttctcaacgc tgctattgat ttgcaggtcg
    12781 atatcctgcg tatcgagtgg ggcgatgcgt atcatcacat ccccagactc aagggtgcct
    12841 gcaacggcgg gctggtttat tttcattttt cacctgtttc tcatgcgggg gtcttttgac
    12901 gagctgccgc gtcctggcgg gagtgctcaa gcaggttctg caaataatgc agcgtgactg
    12961 cagggaccag cggcgcgata gccgtgagat cgtttttcgc cagcagttga cgtacccggg
    13021 aagcggatat cggcatctcc tggtaacgca gccgctcaat ttcaaccagt tcgatgggcg
    13081 gtgcggagat agtcggcgtt tccagccagt agcgcatatc ctggttgtac tgggcggtaa
    13141 cgcgacaaaa gggttcagta ccgacaaagc ggtgagttac acccagcgcg ggagcgaggt
    13201 actgacggaa aatcttcaga tcaatttcgg tgtaacaatg gttaatgacg ctctgttctt
    13261 taatgaagta gcaagggaac gtagcgcggg agatgatgta ttcggagccg cgatgcacag
    13321 tcaggcgtgg aatatcggcg gtgcctttta acaccagatc caaccggtct tcatagggga
    13381 agcgtgaaga atcttctttg actaaaaaca gatgcaacca gtcgcactgt gccgcagctt
    13441 gttgaatcag ataacggtga ccattcgtga agggattggc gttcatcaca atgcagccaa
    13501 tcttgttccc tggatgacga aattttttca gcgattcggc atagcgtttc agccgcgtgg
    13561 cgctgttttc catcagcacc atcacgccgg gtacgctggt cagcgtggaa aaaccgcact
    13621 ggcggaacag cgcctcgtat tcggttttgg tataaataaa cagatgcgtg ctgtgccgct
    13681 cataggcgag gtttatcaat tcagtggcta atgtcagcgc cagtccttca ccgcgaacgg
    13741 attcactgat agcaacgcat ttaataatat ttccggca
//
LOCUS       DAJTVK010000111        13771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000111 DAJTVK010000000
VERSION     DAJTVK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.111"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OO372_003250"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="OO372_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6342662.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OO372_003251"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="OO372_003251"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6342663.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="OO372_003252"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="OO372_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6342664.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OO372_003253"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="OO372_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6342665.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5099..6046)
                     /gene="prs"
                     /locus_tag="OO372_003254"
     CDS             complement(5099..6046)
                     /gene="prs"
                     /locus_tag="OO372_003254"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6342666.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="OO372_003255"
     CDS             complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="OO372_003255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6342667.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="OO372_003256"
     CDS             complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="OO372_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6342668.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7885..9141
                     /gene="hemA"
                     /locus_tag="OO372_003257"
     CDS             7885..9141
                     /gene="hemA"
                     /locus_tag="OO372_003257"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6342669.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9183..10265
                     /gene="prfA"
                     /locus_tag="OO372_003258"
     CDS             9183..10265
                     /gene="prfA"
                     /locus_tag="OO372_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6342670.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10265..11098
                     /gene="prmC"
                     /locus_tag="OO372_003259"
     CDS             10265..11098
                     /gene="prmC"
                     /locus_tag="OO372_003259"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6342671.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11095..11487
                     /locus_tag="OO372_003260"
     CDS             11095..11487
                     /locus_tag="OO372_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6342672.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11491..12299
                     /locus_tag="OO372_003261"
                     /pseudo
     CDS             11491..12299
                     /locus_tag="OO372_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            12335..13189
                     /gene="kdsA"
                     /locus_tag="OO372_003262"
     CDS             12335..13189
                     /gene="kdsA"
                     /locus_tag="OO372_003262"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6342673.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13338..13445)
                     /locus_tag="OO372_003263"
     CDS             complement(13338..13445)
                     /locus_tag="OO372_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6342674.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3451 a         3430 c         3514 g         3376 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaacct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt
     5101 agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca
     5161 gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg
     5221 gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt
     5281 tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca
     5341 gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc
     5401 agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac
     5461 gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac
     5521 gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca
     5581 tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct
     5641 gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga
     5701 agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc
     5761 gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg
     5821 catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga
     5881 tgatgaaaat atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc
     5941 taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg
     6001 ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag
     6061 gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt
     6121 ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa
     6181 actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg
     6241 ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg
     6301 tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta
     6361 acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat
     6421 cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga
     6481 gttcaggatc tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat
     6541 accacttctc tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg
     6601 catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct
     6661 catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat
     6721 tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg
     6781 caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg
     6841 cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat
     6901 ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg
     6961 tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt
     7021 ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt
     7081 gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt
     7141 gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct
     7201 caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca
     7261 ttggcgcaag ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc
     7321 gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc
     7381 attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg
     7441 gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa
     7501 cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg
     7561 tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac
     7621 aagagcagcc agcggtagca ggcggataag acgaaaatcg ggctggggca tagtgatgac
     7681 aagtccttga gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt
     7741 ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg
     7801 gggcgctctc ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca
     7861 acgttggtat tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg
     7921 gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt
     7981 gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc
     8041 acggaacttt atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg
     8101 ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag
     8161 gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt
     8221 ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat
     8281 atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt
     8341 cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg
     8401 gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa
     8461 accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc
     8521 aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc
     8581 ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc
     8641 ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa
     8701 ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg
     8761 gcgaatgctt atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg
     8821 cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt
     8881 atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca
     8941 gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac
     9001 gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca
     9061 acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc
     9121 gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc
     9181 ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc
     9241 aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac
     9301 gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg
     9361 aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg
     9421 atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt
     9481 tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg
     9541 gcggcgacga agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg
     9601 aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata
     9661 aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg
     9721 gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg
     9781 cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag
     9841 cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca
     9901 ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg
     9961 aacgttcaca acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg
    10021 ctgcagaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga
    10081 gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc
    10141 accgcatcaa cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc
    10201 tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg
    10261 aataatggaa tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag
    10321 cccacgccgt gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat
    10381 tctggccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact
    10441 gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc
    10501 gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt
    10561 ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg
    10621 taccggggcg attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt
    10681 agatcgtatg cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa
    10741 aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat
    10801 gattgtcagc aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt
    10861 ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca
    10921 tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg
    10981 ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga
    11041 aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca
    11101 agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta
    11161 accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc
    11221 attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa
    11281 gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt
    11341 atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag
    11401 gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc
    11461 accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat
    11521 aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc
    11581 tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc
    11641 cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg
    11701 ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg
    11761 ttaaagaatc gacaggcagt gcggtatcat taggtgcggt tttattatgg gtcgcgaatc
    11821 gtctcgattt gccgctgctg ccggtgattt tccctacgca gctgatattg cgcattgaat
    11881 gtccggatgg cgaaatttgg ctgattaatc cttttaacgg tgaatcgtta agcgaacata
    11941 tgctggacgt atggttaaag ggaaatatca gcccgtcggc ggaactgttt tatgaagacc
    12001 ttgatgaagc tgataacatt gaggtaatcc gcaaattgtt ggatacactc aaagcctcgt
    12061 tgatggaaga aaatcagatg gagctggcgt tacgcaccag cgaagcttta ttacaattca
    12121 acccggaaga tccctatgaa attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg
    12181 aacacgttgc gttgaacgat ttaagttatt tcgttgaaca gtgtccggaa gacccgatca
    12241 gcgaaatgat ccgtgcgcaa ataaataaca tcgcgcataa acatattgtg ctgcattaat
    12301 taatcgacat tttactcaag attaaggcga tcctatgaaa caaaaagtgg ttagcattgg
    12361 cgacatcaac gtagcaaatg acctgccgtt cgtactgttt ggtggtatga acgtgctgga
    12421 atctcgcgat ctggcgatgc gcatttgcga gcactacgta accgtgaccc agaaactggg
    12481 tatcccttac gtgttcaaag cctcttttga caaagccaac cgctcctcca tccactctta
    12541 tcgtggaccg ggcctggaag aagggatgaa aatcttccag gagttgaagc agacttttgg
    12601 cgtgaaaatt atcaccgacg ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt
    12661 ggatgtgatt cagttgccgg cgtttcttgc tcgccagact gacctggttg aagccatggc
    12721 gaaaaccggt gcggtaatta acgtcaagaa accacagttt gtcagcccgg gacagatggg
    12781 taatatcgtt gataaattca aagaaggcgg caacgaaaaa gtgattcttt gcgatcgcgg
    12841 tgctaacttc ggctatgaca acctggttgt cgatatgctg ggcttcagca ttatgaagaa
    12901 agtgtctggt aactcgccgg tgattttcga cgtgacccac gcactgcaat gccgcgatcc
    12961 gtttggcgca gcttccggtg gtcgtcgtgc tcaggtggct gagctggcac gagccggtat
    13021 ggcggtaggt ctggcggggc tgtttattga agcgcatccg gatccggaac atgcgaaatg
    13081 tgatggtcca tccgcgctgc cgctggctaa actggaaccg ttcctcaagc agatgaaagc
    13141 gattgatgat ctggtgaaag gtttcgaaga actggatacc agcaagtaat cttttttgct
    13201 tgaaaaataa agccttagcg ttctgcgtta agactttttt catgggtgcc agatacaaaa
    13261 aaggccgcag gctgttaccc ctgcggccgg tttcgggcgc atattgccat cacggcagcc
    13321 tgacgcccgt tttcacctta cttccggtta cgccaccagc cgacaatcgc tgcggtaata
    13381 attcccgcca ggatcggtgc tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc
    13441 gtcatatagc cgccttgttg taatgacaac attttgcggc tattcttgaa ttgttctggt
    13501 tcaagattag cccccgttct gttgtcaggt taaacctctc aacgtgcggg ggttttctct
    13561 ttccagcaac caatgccacc agggataaag cccccgcaac attgcgcctc accggataac
    13621 gccggcttga tgtggatact acgtctcaat tcatcttcac ttcatccctg aaatgtttgc
    13681 aatgaagagt gcattccggt ttttcaacag ctgttacagt cgtttcatga gtgctctgga
    13741 tgatgcttcc agctcgggtt gccaatattt a
//
LOCUS       DAJTVK010000112        13760 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000112 DAJTVK010000000
VERSION     DAJTVK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.112"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="OO372_003264"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="OO372_003264"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6342675.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="OO372_003265"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="OO372_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6342676.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="OO372_003266"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="OO372_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6342677.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="OO372_003267"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="OO372_003267"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6342678.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="OO372_003268"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="OO372_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6342679.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="OO372_003269"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="OO372_003269"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6342680.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="OO372_003270"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="OO372_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6342681.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="OO372_003271"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="OO372_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6342682.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="OO372_003272"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="OO372_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6342683.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="OO372_003273"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="OO372_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6342684.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSSVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="OO372_003274"
     CDS             complement(11403..11900)
                     /locus_tag="OO372_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6342685.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="OO372_003275"
     CDS             complement(11897..12259)
                     /locus_tag="OO372_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6342686.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="OO372_003276"
     CDS             complement(12249..12596)
                     /locus_tag="OO372_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6342687.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /locus_tag="OO372_003277"
     CDS             12705..13154
                     /locus_tag="OO372_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342688.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13403..>13760)
                     /locus_tag="OO372_003278"
     CDS             complement(13403..>13760)
                     /locus_tag="OO372_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6342689.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3267 a         3817 c         3569 g         3107 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg tggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattgc tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc agcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cagcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctagggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtggc cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc
//
LOCUS       DAJTVK010000113        13589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000113 DAJTVK010000000
VERSION     DAJTVK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.113"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="OO372_003279"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="OO372_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR6342690.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="OO372_003280"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="OO372_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR6342691.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /gene="maeA"
                     /locus_tag="OO372_003281"
     CDS             587..2284
                     /gene="maeA"
                     /locus_tag="OO372_003281"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6342692.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="OO372_003282"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="OO372_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="OO372_003283"
     CDS             3566..3850
                     /locus_tag="OO372_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6342693.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO372_003284"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="OO372_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6342694.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO372_003285"
     CDS             complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="OO372_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6342695.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8667)
                     /gene="fdnG"
                     /locus_tag="OO372_003286"
                     /pseudo
     CDS             complement(5621..8667)
                     /gene="fdnG"
                     /locus_tag="OO372_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="OO372_003287"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="OO372_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6342696.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            10039..11139
                     /gene="ompC"
                     /locus_tag="OO372_003288"
     CDS             10039..11139
                     /gene="ompC"
                     /locus_tag="OO372_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6342697.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            11303..11785
                     /locus_tag="OO372_003289"
                     /pseudo
     CDS             11303..11785
                     /locus_tag="OO372_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11900..12448
                     /locus_tag="OO372_003290"
                     /pseudo
     CDS             11900..12448
                     /locus_tag="OO372_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            12556..13587
                     /locus_tag="OO372_003291"
     CDS             12556..13587
                     /locus_tag="OO372_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR6342698.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT         3542 a         3393 c         3275 g         3379 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgcctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     9901 aaataatatt atttcatcta tttttctcat tgtttttaat caccattcta ttttaaaaac
     9961 agtatgtcat ttataaatga cgccgtttaa aattcgttta gaaaatacgt ttaacgataa
    10021 ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg tggttgtaac tggtttgtta
    10081 gctgcgaacg tagcacacgc tgccgaagtc tataacaagg atggtaataa actcgacctt
    10141 tatggcaagg ttaccgctct acgttatttt actgatgata agcgtgacga tggtgataaa
    10201 acttatgccc gtctcggctt taaaggagaa acgcaaatca atgatcaaat gattggtttt
    10261 ggtcactggg aatatgattt taaaggctat aacgatgaag ccaacggctc gcgcggcaac
    10321 aagacccgtc tggcctatgc tggtttaaaa attagtgaat ttggctctct ggactatggt
    10381 cgtaactacg gtgtcggcta cgacattggt tcatggaccg atatgttgcc agaatttggt
    10441 ggcgatacct ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc
    10501 tatcgcaact acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa
    10561 ggcaaaaatg aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac
    10621 ggtgacggtt tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta
    10681 tataccaaat ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc
    10741 tccggtaaga atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac
    10801 tttgcagcta attacgctga aacattaaac atgaccacct atggggatgg ttatatctct
    10861 aacaaagcac aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca
    10921 tcactcgctt acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg
    10981 attgagtata tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt
    11041 gattataaaa tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc
    11101 gataacatcg tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct
    11161 tctctgggct ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa
    11221 tttttgatat ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc
    11281 ttactataat atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa
    11341 taatgccagt tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt
    11401 ttgtgaacga ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat
    11461 tgccgtggag cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata
    11521 tcttgatttg agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa
    11581 caattgtcta acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag
    11641 cgttaataaa attaacacga taacctttcc aaaagaatca aaactggaaa acatttatat
    11701 tgatcataac aatctggaaa gtttagattt aaaaaatcag cattcattgg ttaatctgga
    11761 agcgcaaaat aacaaactga caaaaattaa tatttctgat agttataaac tgaaatttct
    11821 taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat ctctgattga
    11881 gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc ccaggatgaa
    11941 aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca ctacaaaact
    12001 ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg atcaactaat
    12061 atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc cacttgcaca
    12121 aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag tgaccattga
    12181 tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca catctatcaa
    12241 tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac tcgtaaagct
    12301 aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa atgtaagtat
    12361 tacacatata tctatgaata ataatgctct cagaaatatt gatatagata ggctttcatc
    12421 agttacttat tttagtgcgg cacataatca actaaatagc tgcgcggaat agtagatcac
    12481 tttgagggaa cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac
    12541 caaccggact gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg
    12601 cagaaagcca tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg
    12661 atgctgcacc ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc
    12721 tctgttggac gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta
    12781 cctgccgggc gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg
    12841 gtaaaacatt ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg
    12901 gcaataaaaa tcaatgagat aaccggttgc cagttaaatg ccggaaccgt tcgccgctgg
    12961 ttgccgtctg cggggattgt gtggcgaagg gctgcgccaa ctctgcgtat ccgtgacccg
    13021 cataaagatg aaaagatggc agcaatccat aaagcactgg acgaatgcag cgcagagcat
    13081 ccggtctttt atgaagatga agtggatatc catcttaatc ccaaaatcgg tgcggactgg
    13141 caactgcgcg gacagcaaaa acgggtggtc acgccgggac agaatgaaaa atattatctg
    13201 gccggagcgc tgcacagcgg gacaggtaaa gtcagctgtg tgggcggcaa cagcaaaagt
    13261 tcggcgctgt tcatcagcct gctgaagcgg cttaaagcga cataccgtcg ggcgaaaacc
    13321 atcacgctga tcgtggacaa ctacattatc cacaaaagcc gggaaacaca gagctggctg
    13381 aaggagaacc cgaagttcag ggtcatttat cagccggttt actcgccatg ggtgaatcat
    13441 gttgaacggc tatggcaggc acttcacgac acaataacgc gtaatcatca gtgcagctca
    13501 atgtggcaac tgttgaaaaa agttcgccat tttatggaaa ccgtcagccc attccccgga
    13561 ggcaaacatg ggctggcaaa agtgtagcg
//
LOCUS       DAJTVK010000114        13260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000114 DAJTVK010000000
VERSION     DAJTVK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.114"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="OO372_003292"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="OO372_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6342699.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO372_003293"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="OO372_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6342700.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO372_003294"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="OO372_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6342701.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="OO372_003295"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="OO372_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6342702.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="OO372_003296"
     CDS             complement(4036..5568)
                     /locus_tag="OO372_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6342703.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="OO372_003297"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="OO372_003297"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6342704.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="OO372_003298"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="OO372_003298"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6342705.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="OO372_003299"
     CDS             complement(8663..10399)
                     /locus_tag="OO372_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6342706.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO372_003300"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="OO372_003300"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6342707.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="OO372_003301"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="OO372_003301"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6342708.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="OO372_003302"
     CDS             complement(12121..12855)
                     /locus_tag="OO372_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6342709.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="OO372_003303"
     CDS             13056..>13260
                     /locus_tag="OO372_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6342710.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3366 a         3370 c         3488 g         3036 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAJTVK010000115        12999 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000115 DAJTVK010000000
VERSION     DAJTVK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.115"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..191
                     /locus_tag="OO372_003304"
     CDS             <1..191
                     /locus_tag="OO372_003304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342711.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            98..1393
                     /locus_tag="OO372_003305"
     CDS             98..1393
                     /locus_tag="OO372_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6342712.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="OO372_003306"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="OO372_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6342713.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="OO372_003307"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="OO372_003307"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6342714.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="OO372_003308"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="OO372_003308"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6342715.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="OO372_003309"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="OO372_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6342716.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="OO372_003310"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="OO372_003310"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6342717.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="OO372_003311"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="OO372_003311"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6342718.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="OO372_003312"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="OO372_003312"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6342719.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3578 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaaggg
//
LOCUS       DAJTVK010000116        12927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000116 DAJTVK010000000
VERSION     DAJTVK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12927
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.116"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..298
                     /locus_tag="OO372_003313"
     CDS             <1..298
                     /locus_tag="OO372_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR6342720.1"
                     /translation="ALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLIS
                     IPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
     gene            793..1692
                     /gene="lysO"
                     /locus_tag="OO372_003314"
     CDS             793..1692
                     /gene="lysO"
                     /locus_tag="OO372_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6342721.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            1836..3488
                     /gene="hcp"
                     /locus_tag="OO372_003315"
                     /gene_synonym="priS"
     CDS             1836..3488
                     /gene="hcp"
                     /locus_tag="OO372_003315"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6342722.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            3500..4468
                     /gene="hcr"
                     /locus_tag="OO372_003316"
     CDS             3500..4468
                     /gene="hcr"
                     /locus_tag="OO372_003316"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6342723.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            4601..6319
                     /gene="poxB"
                     /locus_tag="OO372_003317"
     CDS             4601..6319
                     /gene="poxB"
                     /locus_tag="OO372_003317"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6342724.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            6356..7357
                     /gene="ltaE"
                     /locus_tag="OO372_003318"
     CDS             6356..7357
                     /gene="ltaE"
                     /locus_tag="OO372_003318"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6342725.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            7368..8798
                     /locus_tag="OO372_003319"
     CDS             7368..8798
                     /locus_tag="OO372_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6342726.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            8897..9910
                     /locus_tag="OO372_003320"
     CDS             8897..9910
                     /locus_tag="OO372_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6342727.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(9907..10737)
                     /gene="amiD"
                     /locus_tag="OO372_003321"
     CDS             complement(9907..10737)
                     /gene="amiD"
                     /locus_tag="OO372_003321"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6342728.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(10734..11057)
                     /locus_tag="OO372_003322"
     CDS             complement(10734..11057)
                     /locus_tag="OO372_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6342729.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(11268..>12927)
                     /locus_tag="OO372_003323"
     CDS             complement(11268..>12927)
                     /locus_tag="OO372_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6342730.1"
                     /translation="RKGELSTWDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFN
                     TFFSNAFETDVKKYLGKSEDNEVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENK
                     LQDQLDKHFTIESHPLLYRHTIGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYD
                     YSRRYAISENIEKDAVTNNIALMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIV
                     LGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCM
                     IHLMVNEEVFMSLPVPVATMVREDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISN
                     LYLSHTVVNESILKKCCFENGSLGDVKITNSNIINNVFKNVSFRSVKINNVNTHSLKF
                     INCHFFNVDMIRVNFSKCLFHDCSMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSIC
                     ARDIYNMSAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDK
                     SIEILLLSCFAASDQKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
BASE COUNT         3065 a         3321 c         3256 g         3285 t
ORIGIN      
        1 cgcgctggta gttgaactgg tattgagtgc aggtttcctg ttggtgatcc acggcgcaac
       61 cgacaaattc gcgccggcag gttttgcgcc gatcgctatt ggtctggcct taaccctgat
      121 tcacttaatt agtattccgg tgactaacac ttctgttaac ccggcgcgca gcaccgcggt
      181 tgctatcttc cagggcggct gggcattaga acaactgtgg ttcttctggg tggtgccaat
      241 tgtcggcggc attatcggtg gtctgattta ccggaccctg ctggaaaagc gtgattaatt
      301 aacgcagaag gcctggcgta atgtcaggcc tttttttaag atgttgttat atgttgattc
      361 atcatcttgt tgcgctcctt tcatgagcta agccacatat tgccactggc gcaaggagcg
      421 cgcagggggc ggccaatcgc cgccgccccc tgcccccccg ggctctggcg aacaaaatcg
      481 ccgctgcgcg gtgccctcga cttatccctt acggctaccg ggtcgggcgc gaggtaacat
      541 ccctgtaaaa cgcgccctca gcccacatcc atgtgggctg ccccggcctt cagggaacgc
      601 ctcggcgatt ttgacgccac caacatcggt gcagtttgta attaaagcgc aagaatatct
      661 ttaaatgaaa acgaatactc tactgctcct tatgcataac atttcctttt aattgcaact
      721 ttactcgtcc ttccgctttc agtagtgtgt catcgcgcat ttcgacttca tctttgcaag
      781 gactgggttt tcatgttttc tgggctgtta atcattctgg ttcccctgat tgtaggttac
      841 ctcattccgc ttcgccaaca agctgcgtta aaagttatta atcagctatt aagctggatg
      901 gtttacctta ttctcttttt tatgggtatc agtctggcgt ttctcgataa cctcgccagt
      961 aacctgttgg cgattctgca ttattctgcc gtcagtatta ccgttatttt actgtgtaat
     1021 attgccgccc tgatgtggct ggagcgaggc ctgccgtggc gcaaccacca tcagcaagaa
     1081 aaactcccgt cgcgtattgc gatggcgctg gagtcgctaa aactgtgcgg cgtagtagtg
     1141 attggttttg ccattggtct gagtggactg gctttcttac aacacgcgac cgaagccagt
     1201 gaatacacgt taattttgct acttttcctc gttggtattc agttgcgcaa taatggcatg
     1261 accttaaagc agattgtcct taatcgccgg ggaatgattg tcgccgtggt ggtggttgtc
     1321 agttcattaa ttggtggttt aattaacgcc tttattcttg atctccccat caataccgcg
     1381 ctggcaatgg cctccggttt cggctggtat tctctttccg gtattttatt gaccgaatct
     1441 tttggtccgg taatcgggag cgcggcgttt tttaatgatc tggcccgtga actgattgct
     1501 attatgttga tccctgggct gattcgccgc agccgctcta ctgcactggg cttatgcggt
     1561 gccacatcaa tggatttcac cctgcccgtt cttcaacgta ctggcgggct ggatatggtc
     1621 ccggcggcaa ttgttcacgg ttttattctt agcctgttag tgccgatcct catcgccttt
     1681 ttctctgcgt aatacctctc tggcggtaga tccctgccgc caaaattgcg ctaaatcaat
     1741 ctcccttaaa gttgcatgaa aaatcccttt tatccccgcg ttaagcgtct taaccttaaa
     1801 catgtatatt aaatataact ttaaaaggtg tgatcatgtt ttgtgtgcaa tgtgaacaaa
     1861 ctatccgtac tccggcagga aacggctgct catacgcgca ggggatgtgt ggtaaaacgg
     1921 cggaaacttc tgaccttcag gatttactca tcgcggcgct gcaagggctt tctgcctggg
     1981 cggtaaaagc gtgtgaatac ggcatcatca accacgatgt tgacagcttc gcgccacgtg
     2041 cctttttctc aaccctgacc aacgttaact tcgattctcc gcgtattgtc ggctacgctc
     2101 gtgaagcgat tgccttgcgc gaggcgctga aagcacaatg cctggctgta gatgccaacg
     2161 cgcgcgtcga taacccgatg gctgacctgc aactggtgag cgatgatctc ggcgagctgc
     2221 aacgtcaggc agcagaattt acccctaaca aagataaagc ggcgattggc gaaaacattc
     2281 tcggcctgcg tctgctgtgc ctgtatggcc tgaaaggtgc ggcggcctat atggaacacg
     2341 cgcatgttct cggtcaatac gacaacgata tttatgccca gtaccataaa atcatggcgt
     2401 ggctggggac ctggcctgcc gatatgaacg cgcttcttga gtgttcaatg gaaatcggcc
     2461 agatgaactt caaagtgatg agcattctgg atgcaggcga aaccggtaaa tacggtcacc
     2521 cgacgccaac ccaggtcaac gtcaaagcga cggcgggtaa atgcattctg atttccggtc
     2581 acgatctcaa agatctctac aacctgctgg aacagaccga aggcacgggc gttaatgtct
     2641 acacccacgg cgaaatgctg cctgcgcatg gctacccgga gctgcgtaaa ttcaagcatc
     2701 tggtcggtaa ctacggcagc ggctggcaga atcagcaagt ggagttcgct cgtttccctg
     2761 gccccatcgt gatgacctcg aactgcatca tcgacccaac cgtaggcgct tatgacgatc
     2821 gtatctggac ccgcagcatt gttggctggc ctggcgtgcg tcatctggac ggtgatgatt
     2881 tctctgcggt tatcacccag gcgcaacaga tggcaggctt cccgtacagc gaaattccgc
     2941 accttatcac cgtgggtttt ggtcgccaga cgctgcttgg cgctgctgat acgctgattg
     3001 atctggtgag ccgtgaaaaa ctgcgtcata tcttcctgct tggtggctgt gacggcgcac
     3061 gcggcgagcg ccactacttc accgatttcg ccaccagcgt gccggatgac tgcctgatcc
     3121 tgaccctcgc ctgtggtaaa tatcgcttta acaaacttga gtttggcgat atcgaaggtc
     3181 tgccgcgtct ggtagatgca ggtcaatgta acgatgctta ctcagcgatt attctggctg
     3241 tcactctggc agagaaactg ggctgtggtg tgaacgatct gccgctgtcg ctggtgctct
     3301 cctggtttga acagaaagcg atcgtcattc tgctgacgct gctttctctg ggcgtgaaaa
     3361 atatcgtcac cggcccgact gcacctggtt tcctgacacc agacctgctg gcggtgctga
     3421 acgagaaatt cggcctgcgt tctatcacca ctgttgaaga agacatgaag caactgttga
     3481 gcgcgtaagg agtttgtcga tgacgatgcc aacgaatcaa tgcccgtggc ggatgcaggt
     3541 tcatcacatt acgcaagaaa cgccggatgt gtggacgatt tccctgattt gccacgatta
     3601 ctacccatat cgcgccgggc aatatgcact ggtcagcgtg cgaaactcag cggaaacgct
     3661 gcgtgcttac accatttcct ccacgccagg cgtgagtgaa tatatcaccc tgaccgtgcg
     3721 gcggattgat gacggtgtcg gctcccagtg gctgacgcgc gatgtaaaac gcggtgatta
     3781 tctctggctt tcggacgcga tgggggaatt tacctgcgac gataaagcag aagataaatt
     3841 cctgttgctg gcggcaggct gcggcgtcac gccgattatg tcgatgcgtc gctggcttgc
     3901 gaagaaccgt ccacaggccg atgtgcgggt gatctacaac gtgcgtacgc cgcaggatgt
     3961 tattttcgcc gatgagtggc gtaactatcc ggtaacgctg gtggcagaaa ataacgttac
     4021 cgaaggcttt atcgctggtc gtctcactcg cgaactgctg gcaggtgtac ctgacttagc
     4081 ttcacgtacc gtgatgacct gcggcccggc tccgtatatg gattgggtag agcaggaagt
     4141 gaaagcgctc ggcgtgacgc gtttctttaa agagaaattc ttcactccag tagcagaagc
     4201 ggcgaccagc ggtctgaaat tcaccaaact gcaaccggca cgagaatttt acgccccggt
     4261 tggcaccacg ctactggagg cgctggaaag caataacgtt ccggttgtcg ctgcctgccg
     4321 tgcgggtgtt tgcggctgct gtaagacaaa agtggtttcc ggtgaatata cggtgagcag
     4381 tacaatgacg ctgaccgacg ccgaaatcgc tgaaggttac gtactggcct gctcctgcca
     4441 tccgcagggg gatttggttc tcgcataatc gccttatgcc cgatgatatt cctttcatcg
     4501 ggctatttaa ccgttagtgc ctcctttctc tcccatccct tccccctccg tcagatgaac
     4561 taaacttgtt accgttatca cattcaggag atggagaacc atgaaacaaa cggttgcagc
     4621 ttatatcgcc aaaacactcg aatcggcagg ggtgaaacgc atctggggag tcacaggcga
     4681 ctctctgaac ggtcttagtg acagtcttaa tcgcatgggc accatcgagt ggatgtccac
     4741 ccgccacgaa gaagtggcgg cctttgccgc tggcgctgaa gcacaactta gcggagaact
     4801 ggcggtctgc gccggatcgt gcggccccgg caacctgcac ttaatcaacg gcctgttcga
     4861 ttgccaccgc aatcacgttc cggtactggc gattgccgct catattccct ccagcgaaat
     4921 tggcagcggc tatttccagg aaacccaccc acaagagcta ttccgcgaat gtagtcacta
     4981 ttgcgagctg gtttccagcc cggagcagat cccacaagta ctggcgattg ccatgcgcaa
     5041 agcggtgctt aaccgtggcg tttcggttgt cgtgttacca ggcgacgtgg cgttaaaacc
     5101 tgcgccagaa ggggcaacca cgcactggta tcatgcgcca caaccagtcg tgacgccgga
     5161 agaagaagag ttacgcaaac tggcgcaact gctgcgttat tccagcaata tcgccctgat
     5221 gtgtggcagc ggctgcgcgg gggcgcataa agagttagtt gagtttgccg ggaaaattaa
     5281 agcgcctatt gttcatgccc tgcgcggtaa agaacatgtc gaatacgata atccgtatga
     5341 tgttggaatg accgggttaa tcggcttctc gtcaggtttc cataccatga tgaacgccga
     5401 cacgttagtg ctactcggca cgcaatttcc ctaccgcgcc ttctacccga ccgatgccaa
     5461 aatcattcag attgatatca acccagccag catcggcgct cacagcaagg tggatatggc
     5521 actggtcggc gatatcaagt cgactctgcg tgcattgctt ccattggtgg aagaaaaagc
     5581 cgatcgcaag tttctggata aagcgctgga agattaccgc gacgcccgca aagggctgga
     5641 cgatttagct aaaccgagcg agaaagccat tcacccgcaa tatctggcgc agcaaattag
     5701 tcattttgcc gccgatgacg ctattttcac ctgtgacgtt ggtacgccaa cggtgtgggc
     5761 ggcacgttat ctaaaaatga acggcaagcg tcgcctgtta ggttcgttta accacggttc
     5821 gatggctaac gccatgccgc aggcgctggg tgcgcaggcg acagagccag aacgtcaggt
     5881 ggtcgccatg tgcggcgatg gcggttttag catgttgatg ggcgatttcc tctcagtagt
     5941 gcagatgaaa ctgccagtga aaattgtcgt ctttaacaac agcgtgctgg gctttgtggc
     6001 gatggagatg aaagctggtg gctatttgac tgacggcacc gaactacacg acacaaactt
     6061 tgcccgcatt gccgaagcgt gcggcattac gggtatccgt gtagaaaaag cgtctgaagt
     6121 tgatgaagcc ctgcaacgcg ccttctccat cgacggtccg gtgctggtgg atgtggtggt
     6181 cgccaaagaa gagttagcca ttccaccgca gatcaaactc gaacaggcca aaggatttag
     6241 cttgtatatg ttgcgcgcaa tcatcagcgg acgcggcgat gaagtgatcg aactggcgaa
     6301 aacgaactgg ctaaggtaaa aagggtggca tttcccgtca taataaggac atgccatgat
     6361 tgatttacgc agtgataccg ttacccgacc gagccgcgcc atgctcgaag caatgatggc
     6421 cgccccggtt ggggacgacg tttacggaga cgaccctacc gttaatgctc tgcaggacta
     6481 cgccgcagag ctttccggta aagaagccgc catttttctg ccgaccggca cccaggctaa
     6541 cctggtcgct ctgctcagtc actgcgaacg cggcgaagag tatattgtcg gtcaggccgc
     6601 gcataattat ctgtttgaag ccggtggcgc agcggtgctg ggcagtattc aaccgcaacc
     6661 catcgacgcg gctgccgacg gcacgctacc gctggataaa gtggcgatga aaatcaaacc
     6721 cgacgatatc catttcgccc gcaccaaatt actcagtcta gaaaacaccc acaacggcaa
     6781 agtgctgccg cgtgaatacc tgaaagaagc atgggaattt acccgcaagc gcaatctggc
     6841 gctgcatgtt gacggtgcgc gcatctttaa tgccgtggtg gcttacggct gcgaactgaa
     6901 agagatcacg caatattgtg attcgttcac catttgcctg tcgaaaggtc ttgggacgcc
     6961 agtcggttca ttactcgtcg gtaatcgtga ttacattaaa cgtgccatcc gctggcggaa
     7021 aatggccggt ggcgggatgc gtcagtccgg gattctggct gccgccggga tgtatgccct
     7081 gaaaaataac gtcgcacggt tgcaggaaga tcacgacaac gccgcctgga tggcggagca
     7141 actgcgtgaa gcaggcgcgg atgtgatgcg tcaggacact aatatgctgt ttgttcgcgt
     7201 cggggaagaa aatgctgccg cgttaggcga atacatgaaa gcgagaaacg tgctgattaa
     7261 cgcctcgccg attgtccgcc tggtgacgca tcttgacgtc tcgcgcgaac aactggcgga
     7321 agtcgccgcc cactggcgcg cattcctggc gcgttaagga gagagacgtg ccgcaacgca
     7381 ttttagttct cggtgccagt ggctacattg gtcagcatct ggtgcgcaca ctcagccagc
     7441 aagggcatca gatcctggcg gcggcacgtc atgtcgacag gcttgcaaag ctgcaactgg
     7501 caaacgtcag ttgccataaa gtcgatctca gctggccgga taaccttccg gccctgttgc
     7561 aggatatcga taccgtctat tttctggtgc acagcatggg cgaaggcggc gattttatcg
     7621 ctcaggagcg ccaggtggct ctcaacgtcc gcgatgcgct acgtgaagta ccagttaagc
     7681 aattaatctt tctcagttcg ttgcaggccc cgccacatga gcagtcggat catctgcgtg
     7741 ctcgtcaggc tacggcggac attcttcgtg aagcgaatgt acctgtgacc gaacttcggg
     7801 ccggaattat cgttggcgca ggttcagcgg cgttcgaagt catgcgcgat atggtctaca
     7861 acctgccagt gttaacgccg ccacgctggg tacgttcacg caccacgccc atcgcgctgg
     7921 aaaacttgct gcactatctg gtggcgttgt tagatcatcc ggccagcgaa caccgcatct
     7981 tcgaagccgc cggaccagag gtgctcagtt atcagcaaca gtttgaacat tttatggcgg
     8041 tgagcggtaa gcgccgctgg ttgatcccca tccccctccc cacccgctgg atttcggtgt
     8101 ggtttctcaa tgtgattact tccgtaccgc ccaccaccgc cagggcgttg attcaggggc
     8161 tgaaacacga tctgctggcg gatgataccg cgctacgtgc actcatccca caacggctga
     8221 tcgctttcga tgacgcggta cgtagcacgt tgaaagagga ggaaaaactg gtcaactcca
     8281 gcgactgggg ctacgacgct caggcctttg cccgctggcg accggagtac ggttattttg
     8341 ccaaacaggc ggggtttacc gttaaaacgt ccgccagcct tgctgcttta tggcaggtag
     8401 tgaaccaaat cggcggtaaa gagcgttatt tctttggcaa tattttgtgg cagacacggg
     8461 cgttgatgga ccgcgcgatc ggtcataagc tggcgaaagg ccgcccggag cgcgaatatt
     8521 tacagactgg cgatgcggtg gatagctgga aagtgattgt cgttgaaccg gaaaaacaac
     8581 ttacgttgtt atttggcatg aaagcgtcgg ggctgggacg actgtgtttt agcctggaag
     8641 ataaaggcga ctatcgtact atcgatgtcc gcgctttctg gcatccgcac ggtatgccgg
     8701 ggctgtttta ctggttattg atgatccccg cgcatctgtt tatttttcgc ggaatggcaa
     8761 aacaaatcgc cagactggca gaacaaagca cagattaatc gacaaaacct gcgttattct
     8821 ttcatgtttc ccattgcatc atgggggaaa tcacggaaga atgcgcacgt cattttctca
     8881 acaacagtgg attgatatga aggtactggt taccggcgcc accagcggct taggtcgaaa
     8941 cgcggtagag tttttatgcc agaaaggcat cagcgtgcga gcgaccggtc gcaacgaggc
     9001 aatgggcaaa ttgctggaaa aaatgggcgc agagtttgtt ccggcggatc tgaccgagct
     9061 ggtttcatca caagctaaag tgatgctcgc gggcattgat acgctgtggc actgctccag
     9121 ctttacctca ccctggggga cacaacaggc tttcgatctg gctaacgttc gcgccactcg
     9181 ccgcctgggt gaatgggctg tcgcctgggg tgtgcgtaac tttattcata tctcttcccc
     9241 ctccctgtac ttcgattatc accaccatcg cgatattaaa gaagattttc gccctcaccg
     9301 cttcgccaac gagtttgccc gcagcaaagc agccagcgaa gaagtgatca atatgctttc
     9361 gcaggcgaat ccacaaacgc gctttactat tctgcgccca caaagtctgt tcggaccgca
     9421 cgataaagtc tttattcccc gtctggcgca tatgatgcac cactacggca gtattctgtt
     9481 accgcatggc ggcagtgcgc tggtcgatat gacctactat gaaaataccg tgcacgcaat
     9541 gtggctggca agccaggaag cctgcgataa gctaccttcc gggcgtgtgt acaatatcac
     9601 caacggcgag catcgcacac tgcgcagcat cgtgcagaag ctgatcgacg agttgaatat
     9661 tgactgtcgt attcgttccg tcccctaccc gatgctggat atgatcgccc gcagcatgga
     9721 gcgtttaggc cgcaagtcag caaaagagcc gccgctgacc cactacggcg tctccaagtt
     9781 gaattttgac tttacgctgg atattacgcg tgcgcaggaa gagttaggtt atcagccgat
     9841 catcacccta gatgaaggta tcgagaaaac tgccgcctgg ctacgcgacc acggaaaact
     9901 gccgcgctaa tcctgcccgt atttctccag caatgcttcg gcaatcgcct gagtttctgc
     9961 atccgcttcg ccgttatata acgtcgggcg gaaatgcatc tggaatgcca taatcacgcg
    10021 ccgctgctcg cgcggtgtca tatcaggttt aacgtcataa ccgtagcgcg ccaaaagctc
    10081 cagcaatgac gcggtatcta ccggtgtgtg cggcgcgcgc ccggcaaggt aaaagttaac
    10141 ccgctgcgca tccggccagg caccaatccc ctgctgcgcc agttgctgcc agggaaataa
    10201 tggccccgga tcgtctttgc gctgcggtgc gatatccgca tgtgccacta cgttttccgg
    10261 cttgatgtga taacgggcaa taatatcttt cgccagtgga ataagcgcct gaatctgtgt
    10321 cggttcaaac ggggcaaaat atttcactcc ggccgatttt tgccagccac ggttttccag
    10381 ctcaatgcca atagaggtgt cgttaaggcg cgttgccccg cgccaggcgc taatccccgc
    10441 atgccaggcc agttcttgtt ccggcaccag ttgccagatg cgcggtttac cgttgtatcg
    10501 cggtggtacc gcagggacca gataatgcga gctgacctgc ttatcggtca gtgtcgccag
    10561 cgagctatca aaatcatctg cggtgtagtg gatcaccagc actttaatgc gcggatacgc
    10621 cgcctgcgcc tggcgtcggg tatcaagctg atatccctct ttctcgacaa tgcctttttc
    10681 gcctgcacac cctgccaata acagagcggc agcgaccagc cagaagactc ttctcatcga
    10741 cgcgttttca ccgccgtacc gctgacgcta accatcagca tactgccgtt ttgcccgacc
    10801 gtttcgtagt cgatatcaat accgacgacg gcatcggccc ccagcgcccg cgcctgggag
    10861 cctaattcct caaaggcgat ctcccgtgct ttacgcagtt ctttttcata cgcaccggaa
    10921 cgtccgccaa cgatatcgcg gataccggca aagaaatcac ggaaaatatt ggcacctaaa
    10981 atcgcttcgc cggtcaccac accgcaatat tcaacgatgg tctggccttc cagagttggg
    11041 gttgtagaaa attgcatgat cgatctcctt gtttgttaag ccttcgatct ctaaagcatt
    11101 atcgaactga tatgccatgt tgggaagaag gttttgatca gtttaggaaa tcatctcatt
    11161 gtgtcaaaaa cacccactta agtctttacg attatccgtt gcataacccg atattggttt
    11221 cattataggg taccgcttac ctgtcttatt atgttgcacc aacaacatta atgagcggaa
    11281 agaggcaact tatattgtaa atgactccta aagtcttccc catcttgtga ctgaggtata
    11341 tacctctgga ttgctttttg gtcagacgcc gcaaaacaag aaagtaataa gatctcaatg
    11401 cttttgtcat taatataagc gaagatcatt cttgcaaatt cgtaaataaa cgcccgttcc
    11461 tgagcgctta atagtttaat gcaatattta ttcttaagag agaaaataat ttcaaggaaa
    11521 gcatccgcag caaccttcac gttattcccg gcgctcatat tatatatatc ccttgcacag
    11581 atagagtaca gagatagtaa acagccatac aaataatcat ttattaactc ttttgtccat
    11641 tttactggca accatggttt tatcttgaca ccatgcatac tacagtcatg aaaaagacat
    11701 ttgctgaaat taactcttat catatcaaca ttaaaaaaat ggcaattaat aaattttaaa
    11761 gaatgagtat ttacgttgtt aattttaaca gatctaaacg aaacattttt gaatacatta
    11821 tttattatat tgctatttgt tattttaaca tcacctaaac tgccgttttc aaaacaacat
    11881 tttttcaata tgctttcatt cacaacagtg tgtgataagt agagattact tatgttttgc
    11941 tttattaatg ccacgtcatt aaaatgacta tatttaatga aaaaaccatc attgagtagt
    12001 gttttaagat tgactaaatt acttgcatct tcacgtacca tcgtggcaac tggaacaggc
    12061 agagacatga aaacttcttc attcaccatc aagtgaatca tgcattgcgt tccctttgtc
    12121 atatccacac atacgctaca aatatcgtgg taatgcgaag agcgcatttc aggaaacaga
    12181 ttaaacacgg tatcttgaaa ggcactaaat cgtgttaata aatcctgata atgaacaggt
    12241 tttgtttcaa aattatcatc agcaaatcca agcactattg tatgctctac atctaacgca
    12301 gtttcaaatg gctcttcttc tgtaaaatca ttatttaaaa cctcacctga cgtattatca
    12361 ctgtcataaa aagtgttcat caatgcgata ttgtttgtta cagcgtcttt ttcgatattc
    12421 tcactaattg catatcgcct gctataatca taaaaatgaa taaacgcgcc ctttataaag
    12481 gatatatatt gttctttgtt aaagtcaata cggctgtata atttttcaaa aatcacattc
    12541 gcaattttac taccaatggt atgcctgtat aataatgggt gcgattcaat cgtaaaatgc
    12601 ttgtctaatt gatcttgtaa tttattctca tagtccagag ggaaaagaaa ctgttgtctt
    12661 tcaagtttcc cattcgatac attacgtatt ccaatagaga ttctttcatt acccgccttt
    12721 atttcaactt cgttatcttc tgatttaccc agatattttt taacatcagt ctcgaaagca
    12781 ttactaaaaa aagtattaaa ttggtaattc gctgatggca tattttccgc gagcaacaca
    12841 tcatttttta tgagatcact gtcagcattt attgctaata tcagatgcaa gtagatggta
    12901 tcccatgtag acaactcacc cttacga
//
LOCUS       DAJTVK010000117        12488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000117 DAJTVK010000000
VERSION     DAJTVK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.117"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            211..423
                     /gene="cspG"
                     /locus_tag="OO372_003324"
     CDS             211..423
                     /gene="cspG"
                     /locus_tag="OO372_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR6342731.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            434..622
                     /locus_tag="OO372_003325"
     CDS             434..622
                     /locus_tag="OO372_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6342732.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            597..827
                     /gene="ymcE"
                     /locus_tag="OO372_003326"
     CDS             597..827
                     /gene="ymcE"
                     /locus_tag="OO372_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR6342733.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            817..990
                     /locus_tag="OO372_003327"
     CDS             817..990
                     /locus_tag="OO372_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR6342734.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1039..2112)
                     /locus_tag="OO372_003328"
                     /pseudo
     CDS             complement(1039..2112)
                     /locus_tag="OO372_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2184..4928)
                     /gene="torS"
                     /locus_tag="OO372_003329"
     CDS             complement(2184..4928)
                     /gene="torS"
                     /locus_tag="OO372_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR6342735.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5011..6039
                     /gene="torT"
                     /locus_tag="OO372_003330"
     CDS             5011..6039
                     /gene="torT"
                     /locus_tag="OO372_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6342736.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6012..6704)
                     /gene="torR"
                     /locus_tag="OO372_003331"
     CDS             complement(6012..6704)
                     /gene="torR"
                     /locus_tag="OO372_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6342737.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6824..7996
                     /gene="torC"
                     /locus_tag="OO372_003332"
     CDS             6824..7996
                     /gene="torC"
                     /locus_tag="OO372_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6342738.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7996..10407
                     /gene="torA"
                     /locus_tag="OO372_003333"
                     /pseudo
     CDS             7996..10407
                     /gene="torA"
                     /locus_tag="OO372_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11046..11351)
                     /gene="cbpM"
                     /locus_tag="OO372_003334"
     CDS             complement(11046..11351)
                     /gene="cbpM"
                     /locus_tag="OO372_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6342739.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11351..12271)
                     /gene="cbpA"
                     /locus_tag="OO372_003335"
     CDS             complement(11351..12271)
                     /gene="cbpA"
                     /locus_tag="OO372_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6342740.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3011 a         3204 c         3269 g         3004 t
ORIGIN      
        1 cggacggcta aaataaaatt tgcttaatct caattatcat gcgttaatag ctgcgtcggt
       61 ttgaaagaca gacagcatac aaagtagttt actaaagcag ttctcattat caggcattat
      121 ccccttcttt tgagtctctc tcctgaacac taagtagttt ctgtattaaa gccctgtttg
      181 ccgaaaggcc caaaatgaag gaagtaaaat atgtctaata aaatgactgg tttagtaaaa
      241 tggtttaacg cagataaagg ttttggcttt atcactcctg atgatggcag caaagacgtt
      301 ttcgtccatt tcaccgccat ccagagcaat gaattccgca cgctgaacga aaatcagaaa
      361 gttgaatttt ctattgagca ggggcaacgt ggccccgcgg cagcgaacgt tgttacgctc
      421 taaggttgcc gttattactc aacatctcca tttccgctgt ccatgttgtc atggttcaca
      481 gtaccgcaca tcggcattcg atgtgacgga gcgaaaccct ttgggcgcta agtgtatttt
      541 ttgtaaatcg acgatgatca cctttgataa cgtcgcgctg caaatacgca ctgaccatgc
      601 gccgctggat ttcacaaaat aatatcaggc tccctcgtgg agcctttttt atatctgcct
      661 tatttttctt caacgctgta tgtatagtaa gcgataacct gttgattatt gaatctttcg
      721 gggagatggc ttataacatt tcttacctga ccagggtacc gggaaccaac accttactgg
      781 cgtgttgctg tcttttaaga ccagaagagg ttaacagtga atattgaaga gttaaaaaaa
      841 caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa tcgccgagct gaacaagaat
      901 acagggaaag aagtctctga aattcgcttc accgcacgag aaaaaatgac cgggcttgaa
      961 agttatgatg tcaaaatcaa aataatgtga ttttgtgaac atcaccccgt gcgaggtgat
     1021 gttccgcttg ttgctaattt agtgaccaat cattggcgct tgtggaatta agcgtcggta
     1081 caattcctcc ggcaccgggc tttgccatac tcccgcatac attgcgtaac caatcaccgc
     1141 gaacataatc ctcagaacca gtagcgtcat taaccagcca gacaacgcaa aagctttttt
     1201 atttgccgca ggtttttgca gtgaaaaggt caatgttgag gctaccggac atgactctac
     1261 gcaagtcata cagccggtac attccactgt tcgtacctga attaatttat ctaccgggat
     1321 ccgtgatggg caattttttg cgcatttgcc acagccgata caactttcgg cattgcgacg
     1381 aatcttaaac ggcgataata gcgaaaccac gcccatcagc gcgccatatg ggcaaagata
     1441 acgacactag gcatggcgaa taaacaggct ggcaatcagc aaaacggtca cgctgattaa
     1501 tgtcgcggtc cccatatgac gaaagaaatc gagcattttt acgtccatca ccacgctgta
     1561 gggcgacaac ataaaatagt gaatcgcctg agcgggcatc aataacgcga tatagagaaa
     1621 aaaactcaac agcaaatact tcacgccgcg cagaggaata tccagccagc ggggaaggac
     1681 acattgccga ccaaacagtt tgttaccgag atcgccgatt aattcagaaa gcgtaccaac
     1741 cgggcataac catgagcaaa aggccttttt gagtaataga ctgatgacga taaaagcgac
     1801 caataacagc atcgcggcgg tgtggacgga cggtaactga cctgttgtaa ggctatattt
     1861 cagattcatc agaccggcaa tcggtagcca gccttcgata cctcccggtc tggcgacaaa
     1921 tgtcgtgcta cttgccgttt cgtaatagcg cacccaatac cagaacgtga tggcaatata
     1981 aatattcatt gccaacagta ataattgcgt cgctttacgc caggtcgtgg cattgcgcca
     2041 gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc cgctgccagc gggttctttt
     2101 tttctctgcc atgattttgc cagtccgtta agttgcatac caaatgccac tattctagtt
     2161 gttcttaact ggctgatatt gattcaaatc gcgttcaggt ctttcttatg caaccatgct
     2221 tccagagcgg caacactgcg tgtaatttct tcgtgtggaa ggggggcaga taatggctgc
     2281 tgctccagtt gtgcgcatag ctggctggcg atacgcattc ccaaactcaa gcaactgctt
     2341 tttagctgat gcgcggcacg ctttattttc tcgctatcct gactggcgcg ggcaatgtcg
     2401 atttcatcga gaagcggcag ggcatgttgt gtaaataata ccagccattc gtggatcttc
     2461 tccgtcccca ttaactgagc atcttcattg agttgcgata catccagcga ttgatcgtta
     2521 ttgacttgca gttggagata gtgcgccagt aactgaccga gcacttcacg cggcaccggt
     2581 ttagggataa tcccgcggaa taatgaactg gtacgctggc gcagcgtttc gtcaatgaca
     2641 tgggcgctaa agccaatcaa aaccagcgac ggatattgct gtgccagttg tcgggcaagc
     2701 gtaatgccgt cgacatccgg cagatcaaaa tccaccagtg cggcagcaaa cggttcgcta
     2761 ttttgcagtg tctctaaagc ctgcgcggca ttgccaacag caacaacctg cgcaccactg
     2821 gtgttcagca tctcaacggt aattcgctgg gttagcgggt tatcttcaat taacagcaaa
     2881 cgtaaaccgt caagacgcac cgcctgattg actgtttttg gcacgggtgc cgtggcaaca
     2941 cgtaacggca agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt
     3001 tcgccgccca tcgcctgggc cagacggcta ctgatagtca gccccagccc ggtgccgccg
     3061 cgtttgccgc ttacctgcac aaatggctgg aagatttctg ccagtttcgc gggatcaata
     3121 ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc catcagtgcg actacgcagg
     3181 ataatgtacc cttcgtcagt aaaacgcagg gcgttgctca acaggttggt tataacctga
     3241 cgaatacgtc gtggatcgcc cattaacgcg cacggcatat catcggcaat tgccgttgcc
     3301 aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt gcagggtact ttccagcagc
     3361 gggcgcggtt caaagggctc atcgctgacc gaaacattct tgccacctgc ttcgatagcg
     3421 gaataatcga gaatatcgtt gaggatggtc agcaacgatt caccagagtc agtaattgcc
     3481 cgcaaatcat cacgctgggc gttaagtgcg gggttatctg ccagtagctg tgcggtgccg
     3541 agaataccgt acagcggtgt gcggatctca tggctcatcg ccgccagaaa cgccgatttt
     3601 gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt gttctatcac tagttcctgc
     3661 aattcagctg tacgcgcttt gacctgcgcc gccagctgtt cacggtggcg attcagtgca
     3721 tgaacactgc tgcgaaacgc acccatcagc tgcccgatgg tgtccagctc ccgtacgcca
     3781 gcggtttccg ggaaagggga gtcaatatca ccgtccagca gccgttgcag cgcctgcgtt
     3841 tgttcggcaa gtggacgcgt gactgagcga taaaccacgc gccagaggat cagaatcagt
     3901 gcgcaaagtg aaaccatccc cagcaataac aggctgtatt gcccgcgtgc actggctttt
     3961 tcctgatgcg ccagtccgtg ctgattacgc agctcaatgg tgtcgaccag ctgactgact
     4021 tcgctactaa actgcgcgaa ctgggcgata ttattttggg cgagagtttg caggtgattg
     4081 ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc tatattggct aacggtagtt
     4141 aacgttgttg cgacctgcgc acgaactccc ggatcttcaa tgcgtatttg ccgacgttgc
     4201 agaattttca ccgcattatt gagctgcttt tccagcgttg gtgcattttt ctggatctgc
     4261 tccagcccca gattcatcac catttgctgc acccgcagag cgctaaggcg cagttcattc
     4321 atctggttaa catactcaag atcgatatca atcagccgat cgagtgcact ttcagcagcc
     4381 tgacgctgat cttgttcgat caaatcgtaa atcccggcct gggtcgctcc agcggaagtt
     4441 gtcgcattat tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc agcgactatc
     4501 tgctgactga gttgctgttg ttgctggcgt agttgcagac gccgccccac cagttcccct
     4561 tgctgacgta acgaacggga gatctcctgc tcctgttgtt caatagcggt ggtatcaaaa
     4621 ccttgttccc gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc ggtgagcatt
     4681 cgcccctgcg cctgccacat cttttcgtta tcggcactgg tcaggttctg cgcggcgaaa
     4741 agttcccagg cgctggcttc gctcaactgg cgcgccatat tcatggtagg aatcaatgcc
     4801 tgagtattgt ctttttccac ctggctgata aagcgcaggt tgtaccatcc caccagggta
     4861 ctggtcaggg ttaacagcgc catcagggca aagcccatcc agagtcttcg ggtcagggtt
     4921 aaattcacgg tcggtgcact ttaggtgaaa aatgttgagt cgcaaagcgg aatgcatcta
     4981 gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc
     5041 cttttcatgt tgccggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat
     5101 ttcacggtgc aagcctctat gccgcttaaa gccaaacgcg catggaaact gtgcgcgctt
     5161 tatcccagcc tgaaagattc atactggtta tcgttgaact atggtatgca ggaggcagct
     5221 cgccgttatg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc
     5281 cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt actcggtagt
     5341 agcacgacct catttcccga tctgcaaaag caggtagcaa gtctgccggt gatcgaactg
     5401 gtaaatgcta ttgatgctcc ccaggtgaaa agccgcgttg gtgtaccctg gtttcagatg
     5461 ggctatcaac ctgggcgata tttggtgcaa tgggcgcacg gtaaaccact gaacgtgctg
     5521 ttgatgcccg gacccgataa tgccgggggc agtaaagaga tggtcgaggg ttttcgcgca
     5581 gccattgccg gaagcccggt gcgtattgtt gatattgcgc ttggtgataa cgatattgaa
     5641 atccagcgta acctgttgca ggagatgctg gaacgccacc cagaaatcga cgtcgttgcc
     5701 ggaacggcca ttgcggcaga ggcggcaatg ggtgaagggc gtaacctgaa aacgccgctt
     5761 accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggctgaagcg gggaagagtg
     5821 attatggctg ccagcgatca aatggtctgg cagggggaac tggcggttga gcaggccatc
     5881 aggcaattac aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agttctgacg
     5941 ccgaaaaatg ccgaccgtga acatattcgc cgctcgctgt caccaggggg atttcgtccg
     6001 gtctattttt atcagcacac atcagcggct aagaaataac cttcaccatg ttgcgtcacc
     6061 agtaaatccg cgctgagttt atgacgtaaa cgacgaatta acacatcgac ggtgcgcagg
     6121 tcagggtttt ccacccgacg cgcagaaagc atacgtagca gacgttcacg gctgagaatt
     6181 tcgcccggat tcgtcacaaa tgccaccaac atttcatact ctgcgcgggt cagtttaatc
     6241 ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc ggcaaagcga
     6301 tagcagttgt cctgagtgtg cggttgagct tgtcgcgcga ggtcgattcg ccagagcaga
     6361 tttttcaccc gtactaccag ttcgcgcagt tccagcggtt tggtgacgta atcgtctgcg
     6421 cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc
     6481 cccaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggtaag
     6541 ttgatatcca gcagaattaa atctaccggc tgattctgca taatttcccg cagcccggca
     6601 ccgctcgctg taacggaaac ggtatacccc tcctgagtga agtaagattg taatcgcgcc
     6661 tgggtaaccg gctcatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca
     6721 ttctgttcat attctgccgt aagccgttca tcctgaccag tgccgctgtt catatttgct
     6781 cattaagatc gcttcactaa accataattt tacaggggct attatgcgga aactctggaa
     6841 cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg ctggtcgcta ttgggattgt
     6901 gattggcatt gcgctgattg tattgccaca cgttgggatc aaagtcacca gcacaaccga
     6961 attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa gaatataaac agtcggtgca
     7021 ttttcagaac gcctctggcg tgcgagctga atgccatgac tgtcatatcc cgccggatat
     7081 tccaggcatg gtgaagcgca aactggaagc gagcaacgac atttaccaga cctttattgc
     7141 ccactccatt gatacacctg aaaaattcga agccaaacgc gcggaacttg ccgagcgtga
     7201 atgggcgcga atgaaagaaa acaactcggc aacctgtcgc tcctgccata actacgatgc
     7261 gatggatcat gccaggcagc atcctgaagc ggcacgtcag atgaaggtgg cagcgaaaga
     7321 taatcaatcc tgcatcgact gtcataaagg tattgcccac cagttaccgg atatgagtag
     7381 cggcttccgt aagcagttcg atgagctgcg cgccagtgct aatgacagtg gtgacacgct
     7441 gtactctatt gatattaagc caatttatgc ggcgaaaggc gataaagaag cctctggttc
     7501 tctgctgcct gcttcggaag tgaaagtcct taaacgtgac ggcgactggc tgcaaattga
     7561 aattaccggc tggacggaaa gcgccggacg tcagcgtgta ctcacccaat tcccaggtaa
     7621 acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag caggtgaaaa cgctggagaa
     7681 aaccaccgtt gccgacacca ataccgagtg gagcaagttg caggccaccg cgtggatgaa
     7741 gaaaggcgac atggtgaacg atatcaaacc gatctgggcc tatgcggatt cgttgtacaa
     7801 cggcacctgt aaccagtgcc acggcgcacc ggaaatcgcc cactttgacg ctaacggttg
     7861 gatcggcacg ctcaacggta tgattggctt taccagcctc gataaacgtg aagaacgcac
     7921 cttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca ggtaaggctc acggcgataa
     7981 gaaggacgaa aaataatgaa caataacgat ctctttcagg catcacgtcg gcgttttctg
     8041 gcacaactcg gcggcttaac cgtcgccggg atgctggggc cgtcattgtt aacgccgcgc
     8101 cgtgcgactg cggcgcaagc ggcgactgag gctgtcatct cgaaagaggg cattcttacc
     8161 gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg gtcgctttgt ggcggcaaaa
     8221 ccgttcgaac tggataaata tccgtcgaaa atgattgccg gattgccgga tcacgtacac
     8281 aacgcggcgc gtattcgtta tccgatggta cgcgtggact ggctgcgtaa gcgccatctc
     8341 agcgatacct cccagcgcgg tgataaccgt tttgtgcgcg tgagctggga tgaagccctc
     8401 gacatgttct atgaagaact ggaacgcgta cagaaaactc acgggccgag tgccttgctg
     8461 accgccagtg gttggcaatc gacggggatg ttccataacg cttcggggat gctggcgaaa
     8521 gctattgcct tgcatggtaa tagcgttggt acgggcggag attactctac cggtgctgcg
     8581 caggtgatcc tgccgcgcgt ggttggctca atggaagtgt atgaacagca aacctcctgg
     8641 ccgctggtat tgcagaacag caaaaccatt gtgctgtggg gctctgattt gctgaaaaac
     8701 cagcaagcga actggtggtg cccggatcac gatgtttatg aatattacgc gcagctaaaa
     8761 gcgaaagtcg ccgccggtga aattgaggtc atcagcatcg atccggttgt cacatccacc
     8821 catgagtatc tggggcgcga gcatgtgaag cacattgcgg ttaacccgca aactgatgtg
     8881 ccgctgcaac tggcgctggc gtatacgctg tacagtgaaa acctgtacga caaaaacttc
     8941 ctcgctaact actgtgtggg ttttgagcag ttcctgccgt acctgctggg tgagaaagac
     9001 ggtcagccga aagatgccgc ataggctgaa aaactgaccg gcattgatgc cgaaaccatt
     9061 cgtgggctgg cgcggcagat ggcggcgaac agaacgcaaa ttattgctgg ctggtgcgtg
     9121 cagcgtatgc agcacggtga acagtgggcg tggatgattg tggttctggc ggcgatgctg
     9181 gggcaaattg gcctgccagg tggtggcttt ggttttggct ggcactataa cggcgcaggc
     9241 acgccggggc gtaaaggcgt tattctgagt ggtttctccg gctctacgtc gattccgcct
     9301 gttcacgaca acagtgatta caaaggctac agcagcacca ttccgattgc ccgttttatc
     9361 gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg gtaaatcggt aaaactgccg
     9421 ccgctgaaaa tgtgtatttt tgctggaact aacccattcc atcgccatca gcagatcaac
     9481 cgcattattg aaggctggcg caagctggaa acggttatcg ccatagataa ccagtggacc
     9541 tcaacctgcc gctttgccga tatcgtgctg cctgcgacca cgcagtttga gcgtaacgat
     9601 ctcgaccagt acggcaacca ctccaaccgt ggcattatcg ccatgaaaca ggtggtgccg
     9661 ccgcagttcg aggcgcgcaa cgactttgat attttccgcg agctgtgccg tcgctttaat
     9721 cgcgaagaag cctttaccga agggctggac gaaatgggct ggctgaaacg catctggcag
     9781 gaaggtgtac aacaaggcaa aggacgcggc gttcatctgc cagcgtttga tgacttctgg
     9841 aataacaaag agtacgtcga gtttgaccat ccgcagatgt ttgttcgcca ccaggcattc
     9901 cgcgaagatc cggatctcga accgctgggc acgccgagtg gcctgattga gatctactcg
     9961 aaaaccatcg ccgatatgaa ctacgacgat tgtcaggggc atccgatgtg gtttgagaaa
    10021 atcgaacgct cccacggtgg gcccggctcg cagaagtatc cgttgcatct gcaatctgtg
    10081 catccggatt tccgacttca ctcgcagtta tgtgagtcgg aaactctgcg tcagcaatat
    10141 acggtagcgg gtaaagagcc agtattcatt aacccgcagg atgccagcgc gcgcggtatt
    10201 cgtaacggtg atgtggtacg cgtctttaac gctcgcggtc aggtgttggc aggggcagtg
    10261 gtttctgacc gctatgcacc cggcgtggca cgaattcacg aaggggcatg gtacgatcca
    10321 gataaaggcg gcgagcctgg tgcgctgtgc aaatacggta accccaacgt gttgaccatc
    10381 gacatcggta catcgcagct ggcgcagttg ttctcccgtg aactggacga tgaacaactg
    10441 acgcaaatcg ccagtgcgca gatggctgaa tggttttcgt tgctgaaaag cgaaccgccg
    10501 ctcactgcgg cggtgaatgc gctggaaaac cgtattgccg ctctgacagt acgtgacgat
    10561 gcccgtctgg aactggccgc tgacttttgc ggcctgtttc tgatgaccga caaacaagcg
    10621 gcgctgccgt atgcatcggc ctacaaacag gacgagcaag agattaaacg cttgttagtt
    10681 gaggcaggga tggaaaccag tggcaatttc aacgaatcgg cagatcatct ggcgatctat
    10741 ctcgagttgt tgagtcatct gcatttttcg ctgggagagg ggaccgttcc tgcgcgaaga
    10801 atcgacagtt tgcggcaaaa aacactgacg gcgctgcggc aatggttacc agagtttgct
    10861 gcgcgttgtc gacagtatga cagctttggt ttttacgcgg cactaagcca gttattgctg
    10921 gtgttagtgg agtgcgacca ccaaaacaga taacgtcgtt tatgcgcctg aaaagacgcg
    10981 tttagcgtcg catcaggcat tatggcgcag ttgccggatg cgtcatcaca tccggcaaac
    11041 acacatcacg gatgagctac aaaccgggaa agccgctggc gtagcaggcg gttttcctgc
    11101 ttcaggtgcg caatatcatc cattaacgtc agcgccaccg cgatccccgg ccagtccaga
    11161 gccagttcat gacgcaggcg taccgcgcgt tgcaccacaa tggcggcatg gtcgtcaaat
    11221 acccaggttg tttcctggat ctcacgcggt tcaaccaccc ccaaaccgac aatttcattc
    11281 aactcctctt cagagatgcc ggtatgcagg caaaattcgg taatagtaaa agtcaccgta
    11341 acattagcca tcatgctttc ccccaatctt tacgtggatc aaaagacgac tgggcgtctg
    11401 ccagttgctg ccacagcgcg gcagtgtttt catccggttt cggcggcatc acgattttca
    11461 gtaccgcata cagatcgccg gtctgttttt tgctcaccag acctttgcct ttaacgcgca
    11521 atcgttgccc ggcctggctg cctggcggga tagtcagcaa aatgctttct ttcagtgttg
    11581 gaacggtgac tttagcaccc agcgccgctt cccacgggct aaccggcacc acaatttcca
    11641 gatcctggcc gacaatatcg aacagcggat gtggcgcaat atgaatcacc agccacaaat
    11701 cgccatttgg accaccgttt tcgcccggcg tcccctggcc tttcagacgg atgcgttgac
    11761 cattgccgac gcccgccggg atcttcacat tcagcgtttt cgggatttcc tgttcgatca
    11821 tgccaaaggc gttataaacc ggcaggttat agctgatggt acgcttatgc tcagtcagcg
    11881 tttcttcgag gaataccgcc acttcgattt caatatcgtg gccgcgtgcg gcggggcgtt
    11941 gacggctctg gcgggcatgc tgaccgaaaa ttgacgagaa gatatcgtca aaatcttcgg
    12001 cattaaaact ctgaccgtcg ccatgatgga actgatggtt aaattgcgga tcgttgcgat
    12061 gttgccacat ctgatcatac tcagcgcgac gttgttcatc acttaacact tcccaggctt
    12121 cagcgacctc tttgaagcgg gcttcggcat ccggttcttt gctgacatca ggatggtatt
    12181 tacgggccag tcgacgatag gcggtcttga ttgtcttgag atcgtccgtc ggtttcacgc
    12241 ccatgatggc gtaataatcc tttaattcca tagcgttatc tcgcgtaaat caacacaaat
    12301 tgaaggaacc cctgtaaggt aactcctata agtgtagggt aatcctcaaa atttcatatg
    12361 ccaacacaga atatgttatt gaaatcatcg cggagaggag gtcgccatca agatgggttg
    12421 ctgaacatat tttaaacagg tgaaaaaggg tgagcgattt ttgatagttg aaccaggcac
    12481 tttaagtt
//
LOCUS       DAJTVK010000118        12409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000118 DAJTVK010000000
VERSION     DAJTVK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.118"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO372_003336"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO372_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6342741.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO372_003337"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO372_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6342742.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OO372_003338"
     CDS             complement(2659..5772)
                     /locus_tag="OO372_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6342743.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO372_003339"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OO372_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR6342744.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OO372_003340"
     CDS             7014..7292
                     /locus_tag="OO372_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342745.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO372_003341"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OO372_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6342746.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO372_003342"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OO372_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6342747.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="OO372_003343"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="OO372_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6342748.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="OO372_003344"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="OO372_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6342749.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="OO372_003345"
     CDS             10284..10931
                     /locus_tag="OO372_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCR6342750.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="OO372_003346"
     CDS             complement(10973..11503)
                     /locus_tag="OO372_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6342751.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO372_003347"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="OO372_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6342752.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2753 c         2808 g         3418 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcc
//
LOCUS       DAJTVK010000119        12230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000119 DAJTVK010000000
VERSION     DAJTVK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12230
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.119"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..201)
                     /locus_tag="OO372_003348"
     CDS             complement(<1..201)
                     /locus_tag="OO372_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCR6342753.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFD"
     gene            complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO372_003349"
     CDS             complement(403..1047)
                     /gene="cheZ"
                     /locus_tag="OO372_003349"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6342754.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO372_003350"
     CDS             complement(1058..1447)
                     /gene="cheY"
                     /locus_tag="OO372_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6342755.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO372_003351"
     CDS             complement(1462..2511)
                     /gene="cheB"
                     /locus_tag="OO372_003351"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6342756.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO372_003352"
     CDS             complement(2514..3374)
                     /gene="cheR"
                     /locus_tag="OO372_003352"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6342757.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO372_003353"
     CDS             complement(3393..4994)
                     /gene="tap"
                     /locus_tag="OO372_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6342758.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO372_003354"
     CDS             complement(5040..6701)
                     /gene="tar"
                     /locus_tag="OO372_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6342759.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO372_003355"
     CDS             complement(6846..7349)
                     /gene="cheW"
                     /locus_tag="OO372_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6342760.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO372_003356"
     CDS             complement(7370..9334)
                     /gene="cheA"
                     /locus_tag="OO372_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6342761.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO372_003357"
     CDS             complement(9339..10265)
                     /gene="motB"
                     /locus_tag="OO372_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6342762.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO372_003358"
     CDS             complement(10262..11149)
                     /gene="motA"
                     /locus_tag="OO372_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6342763.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO372_003359"
     CDS             complement(11275..11853)
                     /gene="flhC"
                     /locus_tag="OO372_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR6342764.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11856..12206)
                     /gene="flhD"
                     /locus_tag="OO372_003360"
     CDS             complement(11856..12206)
                     /gene="flhD"
                     /locus_tag="OO372_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR6342765.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2792 a         3422 c         2988 g         3028 t
ORIGIN      
        1 atcaaaatgc agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc
       61 aaaccagata acacacacgc ccactaacaa aatcagcaac gaggtcagtt cacgggaacg
      121 cgggatttgc ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt
      181 gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga gtattaagct taaatgatgc
      241 cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa caggcgggat ttagggcttt
      301 tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga
      361 ccgcctgata tgacgtggtc acgccacatc aggcaataca aatcaaaatc caagactatc
      421 caacaaatcg tccacctgat cctgactggc taccacgccg actttgctgg tatcgacctg
      481 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
      541 ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca tcatccgctt
      601 aatgaccagc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
      661 gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag
      721 ttcacgggca tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
      781 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
      841 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
      901 ggggatggct tccgccgctt cggcaatggc ctgatccagc cccagttccc gcaaactgtc
      961 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     1021 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     1081 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg
     1141 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     1201 ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc aggccatcca
     1261 tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     1321 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     1381 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     1441 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccaa
     1501 catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca tattgatggc
     1561 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     1621 cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     1681 cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat ctaccgaagg
     1741 ccgatgacgg ttaaccgccg ggccgtcgtg aattttgatt tgataatttg cgccactacg
     1801 cgccagttcc atatgccgat cgcccggcgc aatataggca tgccccggca agacacgttc
     1861 tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     1921 gcgggtgaaa ccgggcggca tatgttgggt aattaacaat gccgggctgg aaagcggcaa
     1981 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     2041 tttttcagaa ctcaacaacg gacccgcctt cagcgttgtc ggtgctgaca atggcttatg
     2101 tgctgcaagg ctcgcctttg ctgccgtacg caccttttca gcaatcattt cgctatacgc
     2161 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg cccccagctc
     2221 cagcgcgcgc agcgtgactt ctgatccttt gccggtcaag gaagaaacca tcacaacggg
     2281 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
     2341 aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg cgaccagcgg
     2401 atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
     2461 ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
     2521 ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag gtggctaaag
     2581 ttttcagagt aacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcggcgc
     2641 aaaatctcct gctgggtatt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
     2701 tcaaacggcc ccggcacggt gtactgtttc gccagtagat tcagcggggc aaaatcaaca
     2761 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
     2821 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg ataaataccg
     2881 cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt ccagcgtccg
     2941 ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
     3001 gaagccgccg cgctccatac gcgatactcg ccagaacggc gatgtgcgtg atccgcgagc
     3061 agagggaaat ggtgtgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
     3121 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata atgaccgaaa
     3181 tccgtcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
     3241 tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
     3301 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
     3361 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
     3421 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
     3481 gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc ttctaccagc
     3541 gaggcgttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac ctgctcaatc
     3601 ccccgctgtt gttcttccga cgcagaggca atttctccca taatgtcgtt cacgcgggtc
     3661 accgaactga caatatcgat catggtcgcg gcggcgttat tcaccagttt cgaaccctgc
     3721 tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc ctgcgcgcta
     3781 cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc ctgctctccg
     3841 gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa agcaattccg
     3901 tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc ctgcatggtg
     3961 tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc attttttgcc
     4021 agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt taattgctcc
     4081 atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact tgagagatcg
     4141 ttattgcctg cgacaatctc cgcgatacca atgtgcatct cctggcttcc cttacgcaca
     4201 tcacttaccg tcccacgcaa agcctgctgc atggtcttca gactggcaaa aatggcggtg
     4261 atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc aatgctgtca
     4321 aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca ccacagcgca
     4381 ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagtgt cgaaatctga
     4441 tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc cagctgccag
     4501 gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt gaggaaatcc
     4561 gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg ccagcgggca
     4621 aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc agtcatcgcc
     4681 ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt cgtcatcaac
     4741 gttttaatgt catccgccgg atagctaagt gccgtcagag tgcccgcttt gttcagcgcg
     4801 gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg ctgattgctc
     4861 tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga catgccgtta
     4921 ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt cgaaattcga
     4981 atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt aaatactcgt
     5041 caaaatgttt cccagtttgg atcttgttca gcaattcgca gtcgtggctg tgccggtggt
     5101 tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct ggctgccaga
     5161 cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc agcggcggcg
     5221 gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat ttccgaaacc
     5281 gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc aatctcgccc
     5341 ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc cccggcgctt
     5401 tcgaccagca ccgaaccggt atcaacgcgt gcgacggagt cttcaatgag ggctttgatc
     5461 tcttttgccg cctgggcgct gcgactggca agattacgca cttcacccgc caccacggca
     5521 aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag cgcgaggata
     5581 ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt cttcgaacta
     5641 tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc gtgctgggcg
     5701 gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc gttttgcttc
     5761 actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga tgcctgctgt
     5821 tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc ggcatagatg
     5881 gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg catatgtgaa
     5941 acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa tggtcagggt attcgctagg
     6001 ttaccaccgg cgatttcgcg aatgtgagca ataatttttg ccagcggagt aagcaacata
     6061 cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc gataaccgcc
     6121 agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc gcgatacagt
     6181 ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg cattccctgg
     6241 gttggctgag cgaaataagc tccagtattg ccataatcta gataatcaat cagttcagtt
     6301 aacgctgtgt aatagttttt atatttttca tcaatattac gactggtagc gaccatttca
     6361 ggtaacggtg ccatgctttt gaatttttta taatgcgtcg ctgcctgcgc caatgttttc
     6421 ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga atccatcatc
     6481 atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc ccaggttgac
     6541 gtcagctcgc cctgctgttc ccgcaattga ttggaaacca caaagctctt ctggctatgg
     6601 tgaagggaag aaaaaaacag actgccggaa ataagctgta acagtgcgaa tacccccagc
     6661 accattacca acagcgtgac tacgcggata cggttaatca taaggcacct tcctgaaaac
     6721 aagttgatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc acccgccgcg
     6781 aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg aggaaatcgg
     6841 gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc gctgttcagc
     6901 agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag tccagtgaga
     6961 tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc cgccgtcaat
     7021 gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc gagattcagg
     7081 acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt aattcgtaag
     7141 tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa cgctggcgtg
     7201 ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag gatatcaata
     7261 ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg ctcgctggcc
     7321 agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt caggcggcgg
     7381 tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca acaatcagtg
     7441 ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg cgatagttac
     7501 tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc agcaaggcgt
     7561 agcggcgacc gccactttgt aagatcacaa caattccctg ggtggcttcg gttttcgcgc
     7621 ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc cgcacttcca
     7681 gtacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc agtgattcca
     7741 taacagcatt cagcggcaga atgaaaactt catccccaac gcgtacggac atgccgtcga
     7801 ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtcccc tgcttcgact
     7861 ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc atgccgacgc
     7921 cgcgcccgga gacgtcggtg acctgctctg ccatggagaa gcctggtgca aatatcagca
     7981 tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag gccgcttttg
     8041 ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg caaatgttgc
     8101 cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct gcggcgagcc
     8161 gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg gtcagcgggt
     8221 cgataatgcg ttctatcagg cttttgtcga gttcagtaga actgcccacc agcgtcagtt
     8281 ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag cgactaaaaa
     8341 catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca cgggcgttac
     8401 gttgtaactg ccccatgctg gttatcaaat caccatgatt aaccgggtcc agttcgctgg
     8461 aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta attaattgat
     8521 caaccttttc taccgctaca cggatgctgg tggattcatt gctgcgcgtc gttttttccc
     8581 gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcactggt ggggtggata
     8641 tttttggcga gacttctact gtttcaaagg taatctgatc ggcttcaatc acaaaacaga
     8701 gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa tattgccgag agcgaatccg
     8761 cccctttcac cacgtcagtt aacgttgtca gatgtcccag ttcttcttcc agcaggtcga
     8821 cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg cgactgactg cgactctgct
     8881 catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat ggcgtttcgc
     8941 ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgaag ctggcggcat
     9001 ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc ttcgtttcca
     9061 aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct tcatcgagca
     9121 ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt cctgcccctc
     9181 ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct ggcgcttccg
     9241 gctgcaaaac cagcagatgc tgctccatgt cagccaacag ttcgtccgct tcatcaaaaa
     9301 atgtctgata aaaatcgctt atatccatgc tcacgctgtc acctcggttc ggctgatggc
     9361 attgtgggaa cactgacctg tggtgcaacc tcaggttttt ccagggcgct tactggctca
     9421 ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt cagtaccagc
     9481 aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg catcgttgcc
     9541 gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat cagttcgcgg
     9601 cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tatatccttt ctcaccgctg
     9661 gcgtagggga aatcatcggt atgacctgaa aggctaatac ggttgggaat accgttcagt
     9721 acaggcgcaa tgacacgcag aatgtcgcgc atatagggtt cgacatcggc actgccggtt
     9781 ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc ctggaccaga
     9841 tcgattttga gatggggacg taacgcccgc agtttcggat cggactctat caactggtcg
     9901 agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag atcttcgatg
     9961 ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc gccgggaatt
    10021 gggctttcac tattagaaat gcgatcgccg cccgtaaccg cagtcgccag tggagtccgg
    10081 aagtactccg caatctgaat cagctctttt gggctggaga tggagatcag ccacatcacc
    10141 agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca cgatccatgt
    10201 gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg cgcttgattc
    10261 ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc acatgctctt
    10321 ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca aactcaacgg
    10381 cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg cactgcatca
    10441 ttttgctggt ttcggcgctt ttctgacgta aaacactcgc taatggggaa ataaatccgt
    10501 aagccaataa aatgccgagg aaagtcccca ccatcgcatg tgcgataagc gcacccagct
    10561 cggcggcagg acgatcggct gaacctaacg cgtgaacgcc ccccattaca gccgcgacaa
    10621 taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg acttctgcct
    10681 cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag gtgttcatat
    10741 gaccgctgat aatcagacgc agataatcga cgataaaatc aagcatgacg ctatccgcga
    10801 ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata tcacgttcca
    10861 gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga gccagcagat
    10921 ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc gccttcagcg
    10981 tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct gcaccggcaa
    11041 taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc atcaaataac
    11101 cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg acatcatcct
    11161 tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga ttggggcaaa
    11221 aaaagcagcg gtacgtcgtt accgctactg gaatgttgcg cctcaccgta tcagttaaac
    11281 agcctgtact ctctgttcat ccagcagttg tgggataata tcggcaggat tctgggaaag
    11341 tttacgtctt tttactgccc gggatggcgg ttgacataag ctgcaggcaa agctgccaac
    11401 aggctggtga gcgtgggtaa taaaattgcc gccgcagcag ttgcagctgg aaagttgcag
    11461 taatccactt tcaacaaacc gcaccaatgt ccaggcacgg gttaatgcca gcagtggtcc
    11521 ttcttctgct tgtgggcact gttcaaggta taaacggtag gctttgatca ccgcatcgac
    11581 gccattacac aaaccggttt tcagtaaaaa ctgccatgca ttacagaaca tcgaagcatg
    11641 aacgttttgt tcccaggtca taaaccagtc ggttgagaat ggcagcatgc ctttcggcgg
    11701 tgggcttccg cgcagttctt tataaagttt tatcaggcgt ccgcgactta actgtgtttc
    11761 gctttccagc atctgcaaac gagcgcccag ggtaatcaat tccattgcca gctgaatatc
    11821 ccgcgcttcc tgaacaatgc ttttttcact catgatcagg cccttttctt gcgcagcgct
    11881 tcttcaggct gattaacatc attcagcaag cgtgttgaga gcatgatgcc ggtatgaatt
    11941 tgctggagat cgtcaacgcg ggaatcttgc gtcaactgag taatcgtctg gtggctgtca
    12001 aaacggaagt gacaaaccag ttgattggtt tctgccagct taaccatttg cggaagagtc
    12061 agtgccgcta acgttgtcgc catttcttca tttatgccga gacgaaacat agcggacgct
    12121 ttgtcctgaa caatcaaacg ctgtgcaagt agtaaatatg acaagttgat gtcataaatg
    12181 tgtttcagca actcggaggt atgcattatt cccacccaga ataaccaact
//
LOCUS       DAJTVK010000120        12038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000120 DAJTVK010000000
VERSION     DAJTVK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.120"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..324
                     /locus_tag="OO372_003361"
     CDS             <1..324
                     /locus_tag="OO372_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342766.1"
                     /translation="AASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLI
                     AIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATP
                     ASNVD"
     gene            458..1535
                     /gene="ygjJ"
                     /locus_tag="OO372_003362"
                     /pseudo
     CDS             458..1535
                     /gene="ygjJ"
                     /locus_tag="OO372_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1552..3903
                     /gene="ygjK"
                     /locus_tag="OO372_003363"
     CDS             1552..3903
                     /gene="ygjK"
                     /locus_tag="OO372_003363"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6342767.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLSMLYNDFFRKQ"
     gene            4129..6147
                     /gene="fadH"
                     /locus_tag="OO372_003364"
     CDS             4129..6147
                     /gene="fadH"
                     /locus_tag="OO372_003364"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6342768.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6192..6608)
                     /gene="higA"
                     /locus_tag="OO372_003365"
     CDS             complement(6192..6608)
                     /gene="higA"
                     /locus_tag="OO372_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6342769.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6605..6919)
                     /gene="higB"
                     /locus_tag="OO372_003366"
     CDS             complement(6605..6919)
                     /gene="higB"
                     /locus_tag="OO372_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6342770.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7204..8340)
                     /gene="rlmG"
                     /locus_tag="OO372_003367"
     CDS             complement(7204..8340)
                     /gene="rlmG"
                     /locus_tag="OO372_003367"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6342771.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8425..8928
                     /locus_tag="OO372_003368"
     CDS             8425..8928
                     /locus_tag="OO372_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6342772.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9005..9688
                     /locus_tag="OO372_003369"
     CDS             9005..9688
                     /locus_tag="OO372_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6342773.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9767..10753
                     /locus_tag="OO372_003370"
     CDS             9767..10753
                     /locus_tag="OO372_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6342774.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11036..12001
                     /gene="alx"
                     /locus_tag="OO372_003371"
     CDS             11036..12001
                     /gene="alx"
                     /locus_tag="OO372_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6342775.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2861 a         3094 c         3226 g         2857 t
ORIGIN      
        1 gcagcgtcca tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa
       61 ttagatcacc tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt
      121 tcaatgctga ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg
      181 aatattctga ccatcatttt ttataacgtc ggtggtattg ttatcttcct tggctttgcg
      241 tggtggaaat acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg
      301 acgccagcca gcaatgttga ttaagcagaa taataaaaaa tgagcaggca ttttccctct
      361 tcctggatga ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa
      421 aaggctgtac tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg
      481 cagagcatta cttgctctgc cgttttgcta cgccttttct gcggcaggag aagaagcacg
      541 tccggcagaa catgacgaca caaaaacacc cgcaattacc tcgacatctt ctccttcatt
      601 tcgtttttac ggcgaattag ggattagggg gtggtggata tatggattta gagggtgaga
      661 ataaacataa atacagcgac ggtacctata ttgaaggtgg cctggagatg aagtacggct
      721 cctggttcgg cctgatttac ggcgaaggct ggaccgtgca ggccgaccac gacggcaatg
      781 cctgggtgcc agaccatagc tggggtggtt tcgagggcgg aattaaccgt ttctatggcg
      841 gttatcgtac caatgatggc accgaaatca tgctcagtct gcgtcaggat tcctcgctgg
      901 atgacctgca atggtggggc gatttcaccc ccgatctggg ctacgtcatt cccaataccc
      961 gcgacattat gactgcgctg aaggtacaga acttaagcgg caactttcgt tatagcgtca
     1021 ccgcgactcc tgccggacat catgatgaaa gcaaagcctg gctacatttt ggcaaatacg
     1081 atcgctatga cgacaaatac acctatccgg caatgatgaa cggttacatc cagtatgacc
     1141 ttgccgaagg catcacctgg atgaacggtc tggaaatcac cgacggcaca ggacagctct
     1201 atctcacggg cctgctaact cctaactttg ccgctcgcgc ctggcaccat accggacgcg
     1261 ccgacgggct ggacgtaccg ggaagtgaaa gtgggatgat ggtgagcgcc atgtatgaag
     1321 cgttaaaggg cgtttatctc tccaccgctt acacctacgc caaacatcgc cctgaccacg
     1381 ctgacgatga aaccacctct ttcatgcagt ttggtatctg gtacgaatac ggcggcggac
     1441 gtttcgccac ggcttttgat agccgcttct acatgaaaaa tgcctctcac gatcccagcg
     1501 accaaatctt cctgatgcaa tatttctact ggtaataagg actgtaattt catgaaaata
     1561 aaaactattt taacgccagt aacctgcgct ctgctgataa gtttttccgc ccatgccgct
     1621 aacgccgaca attataaaaa cgtgattaac cgtactggcg cgccgcagta catgaaggat
     1681 tacgattacg acgatcacca gcgttttaat ccgtttttcg atctcggagc ctggcatggt
     1741 catctgttgc cagacggccc taacaccatg ggcggctttc cgggcgttgc gctgctgacg
     1801 gaagagtaca tcaactttat ggccagcaat ttcgaccgcc tgaccgtctg gcaggacggc
     1861 aagaaagtcg actttacgct ggaggcatac agtattcccg gtgcgctggt gcaaaaactg
     1921 acagcaaaag atgtgcaagt cgaaatgact ctgcgcttcg ccacgccgcg cacgtcacta
     1981 ctggaaacca aaatcaccag caataaaccg ctggatctgg tgtgggacgg cgaactgctg
     2041 gaaaaactgg aagcgaaaga agggaaaccg ctttccgata aaaccattgc tggcgaatac
     2101 cctgactatc agcgcaaaat cagcgccacc cgtgatggcc tgaaagtcac ctttggcaaa
     2161 gtgcgcgcca cctgggatct gctgacctcc ggcgaatcgg aatatcaggt gcataaatcc
     2221 ctgccggtgc agactgaaat caacggtaat cgctttacca gtaaggcgca tatcaacggt
     2281 tcgaccacgc tctacaccac ctattcccat ctgctgaccg ctcaggaagt tagcaaagag
     2341 caaatgcaga tccgcgatat tctggcgcgt ccggcgtttt atctcaccgc ctcgcagcaa
     2401 cgctgggaag aatacctgaa gaaagggtta accaatccgg atgcgacgcc ggaacagacg
     2461 cgcgtcgcgg tgaaagcgat cgaaacgctc aacggtaact ggctctcacc gggcggtgcg
     2521 gtgaaattta acaccgttac accgtcggtg accgggcgct ggttctccgg caatcagacc
     2581 tggccgtggg atacctggaa gcaggcgttt gcgatggcgc atttcaatcc ggacatcgcc
     2641 aaagagaata tccgcgcggt cttctcctgg cagatccagc ctggcgatag cgtgcgtccg
     2701 caggatgtgg gctttgtccc cgacctgata gcgtggaacc ttagccccga gcgtggtggc
     2761 gatggcggca actggaacga acgtaatacc aaacccagcc ttgccgcctg gtcggtgatg
     2821 gaagtgtaca acgtcaccca ggataaagcc tggttggcag agatgtaccc gaaactggtg
     2881 gcctatcacg actggtggtt acgtaaccgc gatcacaacg gcaacggcgt gccggaatat
     2941 ggcgcgaccc gcgacaaagc ccacaacact gagagcggcg agatgctgtt tacggtgaaa
     3001 aaaggcgata aagaagagac gcagtctggg ctgaacaact acgcccgcgt ggtggagaaa
     3061 ggcctgtacg acagtctgga aattccggca caggttgctg catcgtggga atcggggcgt
     3121 gatgatgccg ccgtctttgg gtttatcgac aaagaacagc tggataaata tgtcgctaat
     3181 ggcggcaaac gtagcgactg gacggtgaaa ttcgccgaaa accgcagtca ggacggaacg
     3241 ttgctgggct actcgctatt gcaggagtcg gtggatcagg ccagctatat gtacagcgat
     3301 aaccattatc tggcggagat ggcaaccatc ctcggtaagc cagaagaggc caaacgctat
     3361 cgccagttgg cacagcagct cgcggactac atcaacacct gtatgttcga cccgactacc
     3421 cagttctact atgacgtacg tattgaagat aaaccactgg cgaacggctg cgcgggcaaa
     3481 ccgattgttg agcgcggtaa agggccggaa ggctggttgc cgctgtttaa cggtgcggca
     3541 acgcaggcca acgccgacgc ggtggtgaag gtgatgctcg atcctaaaga gttcaacacc
     3601 tttgtcccgc tgggaacggc ggcgttaacc aatccggctt ttggcgctga tatctactgg
     3661 cgtgggcgcg tatgggtgga tcagttctgg tttggtctga aagggatgga gcgttacggt
     3721 tatcgtgatg atgccctgaa gctggcggat acgttcttcc ggcacgccaa aggattaacc
     3781 gccgatggcc caattcagga gaattacaac ccgctgactg gcgcacagca aggcgcacca
     3841 aatttctcct ggagtgccgc tcatttgtct atgttgtata acgatttttt ccgtaagcag
     3901 taatttgcaa cggctggcgg atgcggcgtg aacgccttat ccgccctaca tgtgcaatcc
     3961 cccaggccgg ataaaacgcg gcaagcgttg catccggcgt taccaccggg cgtattcttt
     4021 ttgaatccca tcacaaaccc cgcactcccc ttttcccttt tctccatcga cggctaaatt
     4081 agaactcatc cgaccacata acaattattt tacatactgg acaattttat gagctatccg
     4141 tcgctgttcg ccccgctgga tttaggtttt accacgttaa aaaaccgcgt gttgatgggc
     4201 tcaatgcaca ccgggctgga ggaatacccg gacggtgccg agcggctggc agcgttttat
     4261 gccgaacgcg cccgtcacgg cgtggcgctg attgtcagcg gcggcattgc accagattta
     4321 acaggcgttg gcatggaagg tggcgcaatg ctcaacgacg ccagccagat cccacaccat
     4381 cgcaccatta ccgaagcggt acatcaggaa ggcggcaaaa tagccctgca aattttgcat
     4441 accgggcgct acagctacca accgcatctg gtcgctccct ccgcattgca ggcccccatc
     4501 aaccgtttcg ttcctcacga actcacccat gaagagatcc tgcaactgat cgacgatttc
     4561 gcccactgcg cgcaactggc acgggaggca ggatacgacg gtgtagaggt aatggggtcc
     4621 gaagggtatt tgatcaacga atttctgacg ctgcgcacca atcagcgtag tgaccagtgg
     4681 ggcggcgatt accgcaaccg gatgcgattt gccgtagaag tagtgcgtgc ggtgcgcgaa
     4741 cgcgtcggca acgacttcat tattatctac cgactgtcga tgctcgacct ggtcgaagac
     4801 ggcgggactt ttgccgaaac ggtagagctg gcgcaggcca ttgaagcggc gggcgcgacc
     4861 attatcaaca ccggcattgg ctggcatgaa gcacgtattc cgaccattgc cacgcccgtg
     4921 ccgcgcggcg catttagctg ggtcacgcgc aaattgaaag gccacgtctc gctgccgctg
     4981 gtaaccacca accggattaa cgatccgcag gttgccgacg atattctctc gcgcggcgat
     5041 gccgatatgg tatcgatggc acgaccgttt cttgctgatg cggaactgct gtcaaaagcg
     5101 caatcggggc gagccgatga gatcaacacc tgcattggct gcaatcaggc ctgcctcgat
     5161 cagatcttcg ttggcaaagt cacctcgtgc ctggtgaatc ctcgcgcctg ccacgaaacc
     5221 aaaatgccaa tccttcctgc cgtgcagaaa aaaaatctgg cggtggtcgg tgcgggacct
     5281 gctgggctgg cgtttgccat taacgcggcg gcgcgtgggc atcaggtaac attgtttgat
     5341 gcccacagcg agatcggcgg gcagtttaat atcgccaaac agatccccgg caaagaggag
     5401 ttttacgaaa cgctgcgcta ttaccaccgg atgatcgaag tgacgggcgt gacgctgaaa
     5461 ctcaatcaca ccgtgacggc ggatcagtta caggcgttcg atgaaacgat cctcgccagt
     5521 gggatcgtgc cgcgcgttcc gcctatcgac gggatcgatc atccgaaggt attgagttat
     5581 ctcgatgtac tgcgcgacaa agcgccggta ggcaacaagg ttgccatcat cggttgtggc
     5641 gggattggtt ttgatacggc gatgtattta agtcagccgg gcgaatccac cagccagaat
     5701 atcgccaggt tctgtaatga gtgggggatc gacagcagcc tacaacaggc tggtggctta
     5761 agcccgcagg gaatgcagat cccgcgtagc ccacggcaaa ttgtgatgct ccagcgcaaa
     5821 gccagcaaac cgggacaggg gttaggcaaa accaccggct ggatccatcg caccaccctg
     5881 ctctcgcggg gtgtgaaaat gatcccaggc gtaagttatc agaagattga cgatgacggg
     5941 ctgcatgtgg tgatcaacgg cgaaacgcag gtattagcag tggacaatgt ggtgatctgc
     6001 gcagggcaag agccaaaccg cgcgctggcg caaccgctga ttgatagcgg gaaaactgtg
     6061 catttaattg gcggctgcga tgtggctatg gagctggacg cacgacgggc aattgcccag
     6121 ggaacacggc tggcgctgga gatttaaatc gttattttaa gcgccggatg caacgcatcc
     6181 ggcacgtcgc gttaatcaat aaacaaggcg ggagaaatgc cgaatcgcgt tgccaatttt
     6241 ttagcgtgtt ccagcgttaa tttcctcttc ccgctcaaaa cgcgtgacac catagattta
     6301 ctgccaattt ccggcagatc ggcaagggtt aaaccatatt gatccataag ggtacgaatc
     6361 acggctatac cgccaggcat ggcttgagcc atggcattaa attccgcaaa ttcgggcgct
     6421 gattcttccc acgcggttat tttggcacac accagatcca gcaaggggtt ttcaggatcg
     6481 ttgagcagca gatgatctac cagttccagc gcctgggtgt attgttcctc gttctgaata
     6541 cccgccagaa aaggtgccac cgcagttagc ttttctcctg cttgcaagat gtcggcaatc
     6601 gcaatcattt tttcccctta gtacgatgaa cagcggtaaa gaaatcgtat tctttatgcg
     6661 tcataacttc acgtatgtag cacttttgcg attcaaaaaa gaccattgct acaacacgta
     6721 attcattgcc cccaacattg aaaacataat gcttatccag atatttgaag ttatccagag
     6781 atgggaatac tgcttttaat gactcaggtt ttttgaaata tcccttagca atcgtgttcc
     6841 ccagagccac caactccgtt ttatgttgcg ggtatttttc cgcagcatct ttcaatgctt
     6901 tttgagttat caggtgcatt cttcatcacg tccgttgaca aattggcaat atgataacat
     6961 ccgttgacag attggcaacg gatgaattta tctgtgtgac ggaagaataa acgtagaaaa
     7021 acgctaaaca agagaatgta gaggatggtg agagggattt tttgttttat ggaagcgcga
     7081 ttccagaact gggcagacat tgccggatgc gacgcttgcc gcgtcttatc cggcctggga
     7141 gtaacgtgct gaacgtaggc cggataaggc gttaacgccg catccggcag gtaactccca
     7201 atcttaccga cgacgcccca gcttcactgc tttcagcacc acaaatttat tattcgtggc
     7261 gatggtggtg cagttgccga aaatcttctt cagtttatgg aagtaatcca ggtgacggtt
     7321 ggcaacaata tacagctcgc cgttgatttt caggcagcgg cgggcatggt ggaacatctc
     7381 ccaggcgacg ttatcggtca gcgcatgttg ttggtgaaac ggcgggttgc agagcacagc
     7441 attaaagcgg aaaggctcca cgccggagag cgcgttgttg atcataaact cgctgcgatc
     7501 taacgcctct ggcatgttgg tttcaacgtt caaacggctg gaagcaaccg ccatcggcga
     7561 ttcatcgaca aacaccactt tcgcctgagg gtttttatca agcagcgtca gaccaataac
     7621 gccattaccg caaccgagat cgacaatctc accttcgaga ttctctggca gatgttgcat
     7681 aaagaagcgt gcgccaatat ccagcccggt gcgggagaag acattcgcat ggttgtggat
     7741 agtccagtca gtaccttcca gcttccagct aacggtctgc ggcgcatcgg ccagcggcgg
     7801 ttcattgaaa gtgcaattaa tcaggcgcgc ttttttccat gccagcgtag tggtggtcgg
     7861 gccgagcact ttctcgaaca gctccagcgt ggaagtgtga atgtcacggg ctttggcacc
     7921 ggcaataata cgtgtatccg acgtgaccac tttgcgcagc gcacgcagtt gctgttccag
     7981 caatgccagt gttttcggca ctttgatcag caccacaccc ggctgttgcg ggtagtcggc
     8041 ggtgctgtcg agaaacttca cgctcgactc atcaatcccg ttgaggcgta aattctcgcg
     8101 cgtcgccagt tcactgatgt atgagtcgcc aatgctgtac ggcttatgtt ccgccagcgc
     8161 gcagcttaac gcaccaaagg catcattcag gatcaacacc gggccgcgga tttctgtgtc
     8221 gtccaactgt tgcagcaaat attcatccgc cgcttcccac gcctgtagcg ggttaacgtc
     8281 atccgtcgcc ggaaaacgtt gtagtgtcag tgaacggaaa ccgttgtcta agtggctcat
     8341 cggccctcct gaatgataaa atttcgacgt tgcccctgaa aagggtgcgt gagtataccc
     8401 tttttctctt tttcgtgggc ggttatgagc aatcttactt atcttcaggg ttatcccgag
     8461 cagctacttt cccaggtgcg aacgctgatt aacgaacagc gtctgggcga tgtgctggca
     8521 aaacgctatc cgggaacaca cgactacgcc accgataaag ccctctggca atatacccag
     8581 gatctaaaaa atcagtttct gcgcaatgcc ccgccgatca ctaaagtgat gtatgacaat
     8641 aagatccacg tactgaaaaa cgcgctcgga ttacataccg ctgtttctcg tgtgcagggc
     8701 ggcaagctga aagcaaaagc agagatccgc gtcgctactg tgtttcgcaa cgcgccggaa
     8761 ccgtttttac gcatgatcgt ggtgcacgaa ctggcgcacc tgaaggagaa agagcataac
     8821 aaagcgtttt accagttgtg ctgccatatg gagccgcagt accaccagct tgagttcgac
     8881 acccggctgt ggctaacgca gttgtcgctt gggcaggaca aaatctgaaa atgatgtcag
     8941 gcgttatctt ttctttcttt agcgcctaaa atcgacctcc cccctttcgt taaggataac
     9001 gaccatgcag ccccgccttc ttctccgtat ttgtttttct cgccggactc taaagattac
     9061 ctgcttactg ttacttgtgg caggagcgac catttttatc gccgatcgcg taatggtcaa
     9121 cgccagcaag cagctgacct ggagcgatgt caacgcagtt ccggcgcgta acgtgggttt
     9181 attgctgggg gcgaggccgg gtaatcgcta ctttacccga cgtattgata ccgcagcagc
     9241 gttgtaccac gtcggaaaag tgaaatggct gttagtcagt ggcgataacg gacgtaaaaa
     9301 ttacgatgaa gcctcaggta tgcagcaggc tttgatcgct aaaggcgtac cggcgaaagt
     9361 gatcttctgc gactatgccg gattctcaac gcttgattca gtggtgcgtg ccaaaaaggt
     9421 gtttggggaa aaccatatca ccattatctc gcaagaattt cataaccaac gcgccatctg
     9481 gctggcaaaa cagtacggca tcgacgctat cgggtttaac gcgccagacc ttaatatgaa
     9541 gcacggtttt tatactcaac tgcgggagaa actcgctcgt gtcagcgcgg tgattgacgc
     9601 caaaatcctc catcgccagc cgaaatatct cgggccatcg gtcatgattg ggccgtttag
     9661 tgagcatggc tgtccggcga aagaataatg cgtatctgcg cacgtcgaag atgaaaaagg
     9721 cgtgctacat tgacgacaga atccctttat ggagtatcca cgcgttatga tacgtttcgc
     9781 tgtgattggt acgaactgga tcactcgcca gttcgtcgag gccgcccatg agagcggtaa
     9841 atacaagtta accgccgtat attcccgcag ccttgaacag gcccagcact tcgccaatga
     9901 tttttctgtc gagcatctgt ttacctcgct ggaagcgatg gcggaaagcg atgccattga
     9961 cgcggtgtat attgccagcc cgaatgccct gcacttttcc cagacacaac ttttccttag
    10021 ccataaaatt catgtgattt gcgagaaacc actggcgtcg aatctggcgg aagtggatgc
    10081 cgccattgcc tgtgcgcggg aaaatcaggt ggtgctgttt gaggcattta aaaccgccag
    10141 cctgccgaac tttcatttgc tgcgccaggc gctgccgaaa gtcggcaaat tgcgtaaagt
    10201 ctttttcaac tattgccagt attcctcgcg gtatcaacgt tacctggatg gtgagaatcc
    10261 caacaccttt aatccggcat tctctaacgg ttcaattatg gatatcggct tttactgcct
    10321 ggcgtcggcg gtggcattat tcggtgaacc gaaaagcgtg caggcaaccg ccagtttgtt
    10381 ggcaagcggc gtagatgccc acggcgtggt ggtgatggat tacggtgatt tcagcgttac
    10441 gttgcagcac tcaaaggtca gtgattctgt cctggaaagc gagattcagg gcgaagcggg
    10501 atcgctggtg attgaaaaac tgtctgaatg ccagaaagtg tgcttcgtgc cgcgtggcag
    10561 ccagatgcag gatctcaccc agccgcagca tattaatacc atgctctacg aagcagagct
    10621 gtttgctacc ctggtggatg agcatctggt ggatcatcca gggctggcgg tcagtcgcat
    10681 caccgccaaa ctgctgactg agatccgccg ccagactggg gtgatttttc cggcagatag
    10741 cgtaaaacta taattgccaa agtaaaacag tgtaaaaggt atgtaacaga ccattgactg
    10801 gctgaatggt ctgtcatact ttgttacctg caaaggggag taacttcatt gccggtcgat
    10861 cgtcattacg atgtgtgaaa aaccacatcc ggtcaccggg caacccgaaa ggaatacgca
    10921 gacgtattcc ttttttgttg taagtgagac cttgccggaa ggcgaggtct atgcataaaa
    10981 agcagcggct gacgtcttcc gacgttggcc gtttttttat gtgtaaggaa cttctatgaa
    11041 tactgtcggc acgccgttgc tatggggcgg attcgctgtt gttgtcgcca ttatgctggc
    11101 tatcgacctg ttgttgcagg ggcgtcgtgg ggcacatgcc atgaccatga aacaggctgc
    11161 ggcctggtcg ctggtctggg tgacgctgtc gttactgttt aacgccgctt tctggtggta
    11221 tctggtgcaa actgaaggtc gcgccgttgc cgatccacag gcactggcct ttctcacggg
    11281 ttatctgatt gagaaatcgc tggcggtcga taacgtcttt gtctggctga tgttgttcag
    11341 ctatttctct gttccggcgg cattacaacg ccgcgtgctg gtgtatggcg tgctcggggc
    11401 gattgttctg cgtaccatca tgatcttcac tggcagctgg ctaatttcgc agttcgactg
    11461 gatcctgtat atcttcggtg ccttcctgct gtttaccggc gtgaagatgg cgctcgccca
    11521 tgaagatgaa tcaggcattg gcgacaagcc gctggtgcgc tggctacgcg gtcatttgcg
    11581 catgaccgac accatcgaca acgagcattt ctttgtgcgt aagaatggat tgctgtacgc
    11641 cacaccgctg atgctggtac tgattctggt ggaattgagc gacgtgattt tcgccgtgga
    11701 tagcattccg gctatcttcg ccgtgaccac tgacccgttc attgtgctga cctcaaacct
    11761 gtttgcgatc ctcggcctgc gtgcgatgta tttcctgctg gcgggcgtag cagagcgttt
    11821 ctcgatgctc aaatatggcc tggcggtgat tctggtgttt atcggtatca agatgctgat
    11881 tgtcgacttc taccatattc caatcgccgt ctcgctgggc gtggtgtttg gcattctggt
    11941 gatgacgttt attatcaacg cctgggtgaa ttatcggcat gataagcagc ggggagaatg
    12001 attcgctgta tattaagcca ttcctagaga taactcat
//
LOCUS       DAJTVK010000121        11877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000121 DAJTVK010000000
VERSION     DAJTVK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.121"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            486..1157
                     /locus_tag="OO372_003372"
     CDS             486..1157
                     /locus_tag="OO372_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCR6342776.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1167..2363
                     /locus_tag="OO372_003373"
                     /pseudo
     CDS             1167..2363
                     /locus_tag="OO372_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2363..2923
                     /locus_tag="OO372_003374"
     CDS             2363..2923
                     /locus_tag="OO372_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6342777.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2925..3698
                     /locus_tag="OO372_003375"
     CDS             2925..3698
                     /locus_tag="OO372_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCR6342778.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3886..4161
                     /gene="frmR"
                     /locus_tag="OO372_003376"
     CDS             3886..4161
                     /gene="frmR"
                     /locus_tag="OO372_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCR6342779.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4196..5305
                     /gene="frmA"
                     /locus_tag="OO372_003377"
     CDS             4196..5305
                     /gene="frmA"
                     /locus_tag="OO372_003377"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCR6342780.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5398..6231
                     /gene="fghA"
                     /locus_tag="OO372_003378"
     CDS             5398..6231
                     /gene="fghA"
                     /locus_tag="OO372_003378"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR6342781.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6457..6996)
                     /locus_tag="OO372_003379"
     CDS             complement(6457..6996)
                     /locus_tag="OO372_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCR6342782.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO372_003380"
     CDS             complement(7098..8309)
                     /gene="mhpT"
                     /locus_tag="OO372_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCR6342783.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO372_003381"
     CDS             complement(8596..9609)
                     /gene="dmpG"
                     /locus_tag="OO372_003381"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCR6342784.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO372_003382"
     CDS             complement(9606..10556)
                     /gene="mhpF"
                     /locus_tag="OO372_003382"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCR6342785.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OO372_003383"
     CDS             complement(10553..11362)
                     /gene="mhpD"
                     /locus_tag="OO372_003383"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCR6342786.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OO372_003384"
     CDS             complement(11372..>11877)
                     /gene="mhpC"
                     /locus_tag="OO372_003384"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCR6342787.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2912 a         3177 c         2908 g         2880 t
ORIGIN      
        1 aatatgcaat tactccgttc taacaattgg gaaaattact acacttcatt ttagtgatgc
       61 ataaacaacc acattacgag atttattact tcattgtttc tatcaataca tataataaaa
      121 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
      181 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
      241 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat accaaacata
      301 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
      361 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
      421 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
      481 attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta
      541 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
      601 gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta
      661 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca
      721 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
      781 tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
      841 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
      901 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
      961 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     1021 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     1081 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     1141 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag
     1201 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     1261 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     1321 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     1381 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt
     1441 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     1501 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     1561 tgctggcaga aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     1621 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     1681 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     1741 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     1801 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     1861 tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga
     1921 ttgtgggcta cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta
     1981 tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta
     2041 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     2101 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     2161 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     2221 ggattatcta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     2281 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     2341 ctgaaaaact atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt
     2401 aattattgat gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg
     2461 tgccgttatt cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt
     2521 tattcgtcct ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa
     2581 aaatgcggtt attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt
     2641 agttgcaaac caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga
     2701 acagcctgtg tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg
     2761 ttgttatatc gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat
     2821 tatttccccg aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg
     2881 aacttattca ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt
     2941 tattagcggc ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg
     3001 cgggctacga ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg
     3061 aactggtggc gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg
     3121 attatgaaac tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga
     3181 ataactggct gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc
     3241 gccaggattt tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg
     3301 gttatcgcta cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat
     3361 cactctatac tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg
     3421 caggtggtag ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt
     3481 atacgcaact atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa
     3541 cacgctacgg cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac
     3601 ttaatctcgg gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg
     3661 gtctgcaact tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc
     3721 ggggcggcct tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt
     3781 caccggataa attttattct ccagcgttat atactatagg gaggtatgca ttgacatata
     3841 gcatacctcc ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg
     3901 gaagagaaga aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg
     3961 gaacggtcgc tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt
     4021 cggggcgcgg ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt
     4081 gaccgaaatg actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg
     4141 gttcgagcct atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa
     4201 atcacgtgct gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt
     4261 tgcaccaccg aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac
     4321 cgacgcattt accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca
     4381 cgaaggggcc ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga
     4441 ccatgtgatc ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa
     4501 aactaacctc tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac
     4561 cacccgtttt tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag
     4621 tgaatacacc gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga
     4681 acacgtctgc ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc
     4741 taaagtccag ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt
     4801 ggttcagggc gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa
     4861 gaaattcgat ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga
     4921 caaaccgatt aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga
     4981 atgcatcggt aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg
     5041 tcagtcggtg atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca
     5101 gttggtcact ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca
     5161 gttaccgggt atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac
     5221 gcataccatg agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc
     5281 cattcgaacc gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg
     5341 gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg
     5401 gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc
     5461 caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa
     5521 aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact
     5581 aaatcgggga tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc
     5641 agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg
     5701 ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc
     5761 cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc
     5821 tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa
     5881 tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag
     5941 caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg
     6001 agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat
     6061 gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat
     6121 atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt
     6181 tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc
     6241 acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag
     6301 gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt
     6361 gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag
     6421 cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac
     6481 tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa
     6541 cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt
     6601 gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat
     6661 caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa
     6721 gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac
     6781 ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc
     6841 acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac
     6901 gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc
     6961 tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata
     7021 atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc
     7081 ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc
     7141 aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg
     7201 accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc
     7261 agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga actgtaaaac
     7321 aacagtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat
     7381 cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac
     7441 atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc
     7501 aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc
     7561 gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc
     7621 accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc
     7681 gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc
     7741 ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac
     7801 accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca
     7861 atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct
     7921 tcagatgtca gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc
     7981 cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca
     8041 aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg
     8101 ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc
     8161 attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga
     8221 tccagccctt ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat
     8281 gaagatgatg aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat
     8341 cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa
     8401 atgtagcgaa atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat
     8461 gcctgatgcg acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc
     8521 ggacaaggcg ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt
     8581 tatcggcagt catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc
     8641 ctcctggccg ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa
     8701 gccataacgc gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta
     8761 tcccagcgcc agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag
     8821 gtcgtcggcg gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc
     8881 cgcggcggca ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag
     8941 gctggcgtcg attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag
     9001 actcaggtta tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag
     9061 ggcgcggaaa cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata
     9121 aatacaggtc gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt
     9181 ggtcatatgg ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg
     9241 aatatgctgg gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc
     9301 gccagcctgc caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt
     9361 cgcgattttg gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg
     9421 tgcgccgaaa ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat
     9481 cgaatccacg cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata
     9541 ctgatgacga atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt
     9601 accgttcatg ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc
     9661 gccagcgcac tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct
     9721 gcgccttcga cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt
     9781 aaattgaccg gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa
     9841 cccggtacgt aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct
     9901 tgtgaagctt cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct
     9961 gggttaagca caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg
    10021 gaagtggttt ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg
    10081 gcgatagaag cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc
    10141 attggaatgg tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc
    10201 gcctcgaggt taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt
    10261 aagcgaatat ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg
    10321 ctggtcgcat caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc
    10381 accccttcat gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga
    10441 ggatcaatgc cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc
    10501 atcagatcgg tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca
    10561 gacttccttt tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat
    10621 gggcttcaaa acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa
    10681 tgatatctcc ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg
    10741 cggcattaag cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat
    10801 tacgcgtcat cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac
    10861 cgccgatgac atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg
    10921 accagtcgcg aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt
    10981 tatacagttc gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga
    11041 tctccgcttc aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat
    11101 aacacatgtc ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca
    11161 ctttcggatg cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat
    11221 attgcacatt tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat
    11281 cgcgcagcgg cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt
    11341 gctcaagagt atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca
    11401 attgattgaa agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat
    11461 gcagttcaga accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc
    11521 ggtcgttgcg cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa
    11581 aatccgggaa ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc
    11641 ggcgcgacag catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat
    11701 cgaaaacgaa gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct
    11761 gattcagtcg cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac
    11821 cgccgcccat cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAJTVK010000122        11876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000122 DAJTVK010000000
VERSION     DAJTVK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.122"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1760
                     /locus_tag="OO372_003385"
     CDS             <1..1760
                     /locus_tag="OO372_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6342788.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="csiD"
                     /locus_tag="OO372_003386"
     CDS             2096..3073
                     /gene="csiD"
                     /locus_tag="OO372_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6342789.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="OO372_003387"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="OO372_003387"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6342790.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="OO372_003388"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="OO372_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6342791.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="OO372_003389"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="OO372_003389"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCR6342792.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8753
                     /locus_tag="OO372_003390"
     CDS             7437..8753
                     /locus_tag="OO372_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6342793.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            8774..9436
                     /gene="csiR"
                     /locus_tag="OO372_003391"
     CDS             8774..9436
                     /gene="csiR"
                     /locus_tag="OO372_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6342794.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OO372_003392"
     CDS             complement(9437..9886)
                     /gene="kbp"
                     /locus_tag="OO372_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6342795.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9970..10128)
                     /locus_tag="OO372_003393"
     CDS             complement(9970..10128)
                     /locus_tag="OO372_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6342796.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10311..10610
                     /locus_tag="OO372_003394"
     CDS             10311..10610
                     /locus_tag="OO372_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6342797.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10620..11144
                     /gene="ygaP"
                     /locus_tag="OO372_003395"
     CDS             10620..11144
                     /gene="ygaP"
                     /locus_tag="OO372_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6342798.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OO372_003396"
     CDS             complement(11191..11595)
                     /gene="stpA"
                     /locus_tag="OO372_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6342799.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2964 a         2928 c         3062 g         2922 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtaag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggccctgtat accgcctcaa
     8281 ggatgctcta ctccttaagc cgtcgcggtg atgctcccgc ggtaatgggt aaaatcaacc
     8341 gcagtaaaac cccgtacgtg gcggtgttac tctccaccgg cgcggcattc ttaacggtgg
     8401 tggtgaacta ttacgcgcct gcgaaggtat ttaaatttct gatcgacagc tccggcgcta
     8461 tcgccctgct ggtttattta gtcatcgccg tttcacagtt gcggatgcgc aaaattctgc
     8521 gagcggaagg aagcgaaatt cgcttgcgaa tgtggcttta tccctggctc acctggctgg
     8581 tcatcggctt tattaccttt gtgttggtag tgatgctatt ccgtccggcg caacagttag
     8641 aagtgatctc caccggctta ttagcgatag ggattatctg taccgtgcca attatggctc
     8701 gctggaaaaa gctggtattg tggcaaaaaa cacccgttca taatacgcgc tgataatgat
     8761 caggagtcac accatgacca ttacgtctct ggatggctat cgctggctga agaacgatat
     8821 tattcgcggt aattttcaac cggatgaaaa attacggatg agtttgctga catcgcgtta
     8881 tgcacttggc gttgggccgt tgcgggaagc actttctcaa ctggtggcgg aacggctggt
     8941 cacggtggtg aatcaaaaag ggtatcgggt ggcgtctatg tcagagcagg agctgctcga
     9001 tattttcgat gcccgcgcca atatggaagc gatgttagtg agtctggcga ttgcccgcgg
     9061 tggcgatgag tgggaggcag acgttctcgc aaaagcgcat ctgctcagta agctggaagc
     9121 ctgtgacgcc agcgagaaaa tgcttgatga gtgggatctg cgtcatcagg cgtttcatac
     9181 agcaattgtg gcgggctgtg gttctcacta tttgctgcaa atgcgtgaac ggttgtttga
     9241 tctggcggcg cgctatcgat ttatctggct gcggcgaacg gtgctttcgg tagaaatgct
     9301 ggaggataaa cacgatcagc accagaccct gactgcggcg gtactggcgc gagataccgc
     9361 gcgcgccagt gagttaatgc gccagcattt actgacgcca attcccatta tccagcaggc
     9421 gatggctggc aattaactac tcttccggaa tacgcaacac ttgccccgga taaattttat
     9481 ccgggctttt tagcatcggt ttattcgctt cgaagatttt attgtacaga ttagcgttac
     9541 cgtagacctg tttggaaatg gcactcagag tgtcgccaga cttaacggta taaaactggc
     9601 tggcagtggc tggtgtcgcc gttttcacct gatcatcaac actggcaata ccggaaatat
     9661 tccccaccgc aacaaggatt ttctccttcg cctcctgact caggccgtca ccagtgaccg
     9721 tcgctttgcc gtcggcaatt tgaatgttca ctttatcagc atcaggtata ccggttttgc
     9781 tcagatgctc ctgcaccttc ttcgcctgat cgtctttatc gtgctgacct gtaaccgcgt
     9841 cccagagttt ttctccggca tctttcacaa aattgaacag acccatagct acctcattcg
     9901 ttaacggaaa cactcagaaa gtgtagcaga ggaacgcggc gcgttaacgc cgcgggggtg
     9961 agcacaggtt taatcgcggg tttgcaccca gaacgcgtga atcaaaccgg ggatataccc
    10021 aagcagtgtc agcagaatat taataatgaa cgcccaaccg aaccctttac cgagcagcac
    10081 gccgagcggc ggtagaatga tggtgatgac gattctccag aaacccatat gtactcccta
    10141 taagaaaatt actcattgtt taaaaagaga ttttatctct taagcgtagt aattttagcg
    10201 gagcctgcca gtttcagctt acgcttttac tctcatttac gctgctgagg ctggtatatg
    10261 ttgcattttt agtttagagc ttacttaatt tagaaaatac ttaaataaat atgactgaac
    10321 tcgcgcaatt acaggccagt gccgaacagg cagcggcctt attgaaagca atgagccatc
    10381 ctaaacgatt gctgattctg tgcatgctta gcggttcccc cggcaccagc gcgggagagc
    10441 tgacgcgcat taccggactg agtgcctctg cgacatcaca gcatctcgct cgtatgcggg
    10501 acgaagggct tatcgacagc caacgggatg cccaacgcat tctatattcc attaaaaatg
    10561 aggcggtaaa tgccattatc gccaccctga aaaatgtcta ttgtccgtaa ggagtcacta
    10621 tggctttgac aaccatttcg ccgcatgatg cacaagaatt aatcgcacgc ggcgcaaagt
    10681 taatcgatat tcgtgacgct gatgaatatc ttcgtgaaca tattcctgaa gcagatctgg
    10741 ctccattatc cgtgctggaa cagtcaggtc ttccacctaa attacgtcgc gagcaaatta
    10801 tattccactg ccaggcaggt aaacgcacca gtaataacgc cgataaatta gcagcgattg
    10861 ccgcccccgc agaaatattt ttactcgaag atgggattga tggctggaaa agagctggat
    10921 tgccagtagc ggtaaataaa tctcaaccct tgccgttaat gcgccaggtg cagatcgctg
    10981 cgggtggttt aatattaatc ggcgttgtac tgggttatac cgtaaatagc ggtttcttct
    11041 tattaagtgg ctttgttggt gccgggttac tgtttgcagg aatcagcggt ttttgtggaa
    11101 tggcaaggtt gttagataag atgccgtgga accaacgagc ttgagaagcg acgccggacg
    11161 cgccctagca gcgacatccg gcctcagtaa ttagatcagg aaatcgtcga gagatttacc
    11221 ttctgccagc gcctgagcaa ttggcttcgg tgtacggccc tgaccggtcc aggttttagt
    11281 ttcaccgtta acatcggtga atttatattt cgccggacgc ggctggcgtt ttttaccagc
    11341 gcgtggtgca gcagcagagc tattacccaa taactcttcc gggttaattc cgtcagcctt
    11401 catcagctcc agccaggtgc taattttttc ctgacgctca gccagttcac gctgctgctg
    11461 ttcttcttct tcacgtcttt ctttagtgac aaccctgaat ttttcgagca tttcttcaag
    11521 aacgtcaatg gagaattcgc gagccatcgc acggagggtg cgaatgttat ttaaactttg
    11581 taacattacg gacataataa agaaaacctt ttaacgccaa aacaaaaaag tatttcgtcg
    11641 cgtagtgtaa gcgatcactt attatatttc cacaaaattt aatctattta cagtcttgcg
    11701 cgagattatt tcagctgtta ttttctgaaa ctctcagaat aatttcaaga ttatatatta
    11761 gccattttga tgagtaattt agattattgg tgaacagaac ggcgctctct gctcgctaat
    11821 tccgcaagcc tgacaaaacc gaagtgaata aaaatgcaat ataacagaat aaaaga
//
LOCUS       DAJTVK010000123        11808 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000123 DAJTVK010000000
VERSION     DAJTVK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.123"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..488
                     /locus_tag="OO372_003397"
     CDS             <1..488
                     /locus_tag="OO372_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6342800.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="OO372_003398"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="OO372_003398"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6342801.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OO372_003399"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="OO372_003399"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6342802.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OO372_003400"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="OO372_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6342803.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="OO372_003401"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="OO372_003401"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR6342804.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OO372_003402"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="OO372_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR6342805.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="OO372_003403"
     CDS             complement(6839..7747)
                     /locus_tag="OO372_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6342806.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="OO372_003404"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="OO372_003404"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCR6342807.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="OO372_003405"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="OO372_003405"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR6342808.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11808
                     /locus_tag="OO372_003406"
     CDS             10715..>11808
                     /locus_tag="OO372_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6342809.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3006 a         2999 c         2926 g         2877 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctacctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagttaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgct
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacat
//
LOCUS       DAJTVK010000124        11779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000124 DAJTVK010000000
VERSION     DAJTVK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.124"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..611)
                     /locus_tag="OO372_003407"
     CDS             complement(<1..611)
                     /locus_tag="OO372_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6342810.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="OO372_003408"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="OO372_003408"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6342811.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="OO372_003409"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="OO372_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6342812.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="OO372_003410"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="OO372_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6342813.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="OO372_003411"
     CDS             complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="OO372_003411"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6342814.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /locus_tag="OO372_003412"
     CDS             6897..7127
                     /locus_tag="OO372_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR6342815.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="OO372_003413"
     CDS             7139..7411
                     /locus_tag="OO372_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6342816.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="OO372_003414"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="OO372_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6342817.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="OO372_003415"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="OO372_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCR6342818.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="OO372_003416"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="OO372_003416"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6342819.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="OO372_003417"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="OO372_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCR6342820.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11779)
                     /locus_tag="OO372_003418"
     CDS             complement(11524..>11779)
                     /locus_tag="OO372_003418"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6342821.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2856 c         2892 g         2987 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gtgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtc
//
LOCUS       DAJTVK010000125        11542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000125 DAJTVK010000000
VERSION     DAJTVK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.125"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            460..963
                     /locus_tag="OO372_003419"
     CDS             460..963
                     /locus_tag="OO372_003419"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6342822.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1336..2529)
                     /locus_tag="OO372_003420"
     CDS             complement(1336..2529)
                     /locus_tag="OO372_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6342823.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="OO372_003421"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="OO372_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6342824.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="OO372_003422"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="OO372_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6342825.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="OO372_003423"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="OO372_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6342826.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="OO372_003424"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="OO372_003424"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6342827.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="OO372_003425"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="OO372_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6342828.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="OO372_003426"
     CDS             complement(11165..11308)
                     /locus_tag="OO372_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342829.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2927 a         2896 c         2949 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tagcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAJTVK010000126        11429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000126 DAJTVK010000000
VERSION     DAJTVK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.126"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..232
                     /locus_tag="OO372_003427"
     CDS             <1..232
                     /locus_tag="OO372_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6342830.1"
                     /translation="ARISPAIQKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVN
                     ITIVAVARELAGFIWDMGRIAMSVAQQPQCHK"
     gene            complement(500..811)
                     /locus_tag="OO372_003428"
                     /pseudo
     CDS             complement(500..811)
                     /locus_tag="OO372_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(830..2707)
                     /gene="bglF"
                     /locus_tag="OO372_003429"
     CDS             complement(830..2707)
                     /gene="bglF"
                     /locus_tag="OO372_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6342831.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2841..3677)
                     /gene="bglG"
                     /locus_tag="OO372_003430"
     CDS             complement(2841..3677)
                     /gene="bglG"
                     /locus_tag="OO372_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6342832.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3963..4688)
                     /gene="phoU"
                     /locus_tag="OO372_003431"
     CDS             complement(3963..4688)
                     /gene="phoU"
                     /locus_tag="OO372_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6342833.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4703..5476)
                     /gene="pstB"
                     /locus_tag="OO372_003432"
     CDS             complement(4703..5476)
                     /gene="pstB"
                     /locus_tag="OO372_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6342834.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5567..6457)
                     /gene="pstA"
                     /locus_tag="OO372_003433"
     CDS             complement(5567..6457)
                     /gene="pstA"
                     /locus_tag="OO372_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6342835.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6457..7416)
                     /gene="pstC"
                     /locus_tag="OO372_003434"
     CDS             complement(6457..7416)
                     /gene="pstC"
                     /locus_tag="OO372_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6342836.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7503..8543)
                     /gene="pstS"
                     /locus_tag="OO372_003435"
     CDS             complement(7503..8543)
                     /gene="pstS"
                     /locus_tag="OO372_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6342837.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8791..9864)
                     /locus_tag="OO372_003436"
     CDS             complement(8791..9864)
                     /locus_tag="OO372_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6342838.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9875..>11429)
                     /locus_tag="OO372_003437"
     CDS             complement(9875..>11429)
                     /locus_tag="OO372_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6342839.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         3010 a         2944 c         2601 g         2874 t
ORIGIN      
        1 ggcacgcatc agtccggcaa tacaaaaaag gcaggaaaat ttaccccgcc ccgtcattga
       61 cagagcatgg gatgctcaac tcaggctttg taagaggtat cgaaaacttc aggccaaagg
      121 aaagaatgtc aatattacaa ttgttgctgt tgcacgtgag ctggcgggtt ttatctggga
      181 tatgggccga atagcaatgt ctgtcgcaca acaaccacaa tgtcacaaat aaaaaatgag
      241 aataaccagt tccctgaagg cgacgtagcc cggtacaaga gtaacccacg ttagcgcttg
      301 gcgacggcgg aacaaagctg attcgcgacg taagaaagtg gcaggctcat atgacggacc
      361 tctgtaatgc ggtatccaac ccgcgaatat cagcgtgatt caccgtcgga tttactgcta
      421 cgtcgccctc agggaattaa acctaaaaag aaaatcgtca aaattagaga ttccggtttg
      481 acacggaaag tcatatcaac gctaaccccg cttcattcgg ttcggcttcg tcgccctgag
      541 ggaaaattcg cgcccaggca atggaaatac gcagacaggt gaagcccatc tcggcaaata
      601 acgcgatatc ttccgggtaa cggtgataaa aatcgatggc gacatctttg atattctctt
      661 tccccaggat gcgcggttcc atttttccca taacgccatg aggctgtaaa tctgaggtcg
      721 tgctcccttt gccatcttcc agccaggcac cttccacctg attggcagct gttgcgccac
      781 cccaaagaaa tgtttctgga aatgctttca taattaactc cttttatcgt tagcgaatga
      841 tggataacag cggttcacct gcgcttatct gcgccgtgcc gtggggtaat acgtctgtaa
      901 aatcatcgct attactgatt aataccggcg tcgtcacatc aaatccggcc tcgcgaatag
      961 cagggatatc aaaagaaatc agccgatcgc ctgtattgac cttgtcaccc acgttgacgt
     1021 gagcggaaaa gaatttgccg tccagtttta cggtgtcgat accgacatga atcaggatct
     1081 ccacaccatc atctgactca atgccaatgg cgtgtaatgt ggcgaacaac gaagcaattc
     1141 gacccgcaac cggagaacgc acttcaccaa ccgagggcag aatggcgata cctttaccca
     1201 acaggccact ggcaaacgtg gtatcagcga cgtgaatgag cggcacaatc tctcccgtca
     1261 tcggtgaaca gataccgccc tgctcaggtg gtgtaataac ctctggtgtt ttctcttgcg
     1321 gggcaccctg cgctggctga cgtttagcgg tgatgaaatg aagcatcacc gtaccgacaa
     1381 atgcgcaacc gatggcaatg acaccgccaa taacgctggc ccagacggtg aaatcaattc
     1441 ccgttgacgg gatggtttgc atgaaggtga aaatacttgg caaaccaaag gagtacactt
     1501 tcgtttgcgc gtagccaatg atggtggccc ccaaagcccc actgatacag gcgataacaa
     1561 aggggtactt acgcggcaga ttaacgccat ataccgctgg ttcggtgata ccaaacaggc
     1621 ccgtcaacgc cgctgatccc gccaccactt ttttctgcgc atcgcgttcg cagaggaaga
     1681 cgccgagcgc cgccccgacc tgcgccataa tggcgggcat taacagcggg atcatggtgt
     1741 cgtagcccag cacggtgaag ttattgatac acagcggcac caggccccag tgcagtccga
     1801 acatgacgaa gatttgccag aagccgccca ttaccgcgcc cgcaaatgca ggaaccgcct
     1861 gataaagcca gagataaccg gcggcaatca gttcgcttat ccaggttgat agcggcccca
     1921 ccagcagaaa ggtgacgggt gtgataacca tcagacatag caatggtgtg aagaaatttt
     1981 tgattgccga cggtaaccac gcattaagtc ggcgttccag aatgctgcac aaccaggcag
     2041 aaaaaataat gggaataacc gatgacgagt aattcaacaa tgtgaccgga atacccagga
     2101 aatccagccc cagcgcatcc gctttttgcc cgttctcgaa agcagtcaga attaatggat
     2161 gcactaacgc tccaccaatc accatggcag taaatggatt accgccaaag cgtttccccg
     2221 cggtgtatcc caggattatc gggaagaacc aaaacaaggc atcactggcg ctgaataaaa
     2281 ttaaataagt accactttgt tcggtcgtcc actgaaaagt gagcgccaga gccagcatac
     2341 ctttcaggat cccggttgcc gccatcaaac cgatcagagg cgtaaaaata cctgaaataa
     2401 cataaacaaa gcggtttagc agattacctt tctcatcatt ttccggtgcc tgttgcgctt
     2461 tttcgccaag gcctgccacg ctgttgaccg ccaggaagac atcggccaca tggttaccta
     2521 tgaccacctg aaactggcca ccgctttcca ccaccataat aataccgggg gtgtttttca
     2581 gtacctcagc ttgcgctttg ctttcatcct ttaacttaaa acgtaatcgc gttgcgcaat
     2641 gcatcagact aacaatgtta tctgcgcccc cgactcctgc gactattttt ctggctaact
     2701 ccgtcataac ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaaaa ctgaaaaaaa
     2761 cggcctgacg tgaatcaagc aatttttttc aggttttgcc cgcttagtgc ggtaacaatc
     2821 ctttactcag taataatatt tcagtgttct ttgcgcacgc gctctatatt tatggctaaa
     2881 aacataatct ctgcgggtga aattttacgt tgatactgca aaccaataaa aatggcgatc
     2941 cgttccgcac attgccatgc ttgcgggtaa ttttgtttta ctgcttgttg taatgattca
     3001 tcactatcgt taattgaagc atgttcaaga atacgccagg ataaaaactt cagatgtgta
     3061 accagtcgct gataactcaa gctttcttcc tggtaattaa ggctgaactg aaattttatt
     3121 aattgcagca tttcgcgcat taactgcgtg acacctgcaa catcctccat atttccgctc
     3181 atttgggcac tgaccagatg catggcaata aagcccactt catctttcgg taactgcacg
     3241 cccaaccgtt tatcaataat ggttaacgct tcttccccta gctggaactc tttcgggtaa
     3301 agccgctgga tatcccacag caacgggttg ggcagcaaca cgttttgctg aaagcgttta
     3361 atcgcaaact ggcaatggtc agttagcgag atataaatac tgtcctgtaa ttttcccaag
     3421 cgctcctgcg ctaaagagat aatacgatca caggttgcca tcacctcaag aggaatatga
     3481 cttaagagtt cgcttaatcg cccgttcagt tcatgactgc tcaaggcata ctctttttct
     3541 attccacttg agttaattct ttcgccagcg catttttgaa agccaattcc tcgccccatg
     3601 acgacttttt cccgctgttg atcatcaata accaccacaa cattattgtt gagaattttg
     3661 gtgatttgca tgttcatagc aaggaccttt tttataaaca aaaaaaaccg acttcaccag
     3721 tattctctgg ttatgtcagg ttttgcctgc gaatgcagta acaatccagt catttattaa
     3781 tgttttttat aacgaacatc caggttcgga aattaattta attgcgtgca gagaaaatag
     3841 caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta ttaactttgt gtaattttag
     3901 gaatttataa agttatatat aacaaatccc aataattaag ttattgggat ttgtctggtg
     3961 aattatttgt cgctatcttt tcccgccagc agtttatcca gctcatcacc gcctacatga
     4021 cggaaatcct gccccttcac gtagtagaag ataaactcac aaatattctg gcagcggtcg
     4081 ccaatacgtt cgatagaacg cgcgcagaac agcgcagtaa gtacgctcgg aatggtacgc
     4141 gaatcttcca tcatgtaggt catcagttga cgaacaatac cttcgtattc ctgatcgact
     4201 tttttatctt cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc
     4261 agcacgtcgt gcagcatctg gatggtatga cggcccagcg actccagact taccagcaac
     4321 ggctgatgct gctgggagaa tttctccagc gcagtacggc agattttgtc cgccacgtcg
     4381 ccaatacgct ccagctcggc aatggtttta ctgatcacca taaccagtcg caggtcgctc
     4441 gccgtcggct gacgtttggc gataatgcgc acgcaggctt catcgatcgc cacttccatc
     4501 atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca gatcgctgtc ctggttatgc
     4561 atcgcggtga ttgcatcaga aagctgctgc tccaccatgc cacccatggt catcacctgc
     4621 gtgcggatac tttccagttc ggcgttgaac tggccggaaa tatgtttatt aagattgaga
     4681 ctgtccataa cgcactcctg aattaaccgt aacgaccggt gatgtagtct tctgtttgtt
     4741 tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt
     4801 acataaacgc cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg gtaacgatca
     4861 ccacggtgta atcctgcttc agttcggtga tcagctcttc aatacgcccg gtagagatgg
     4921 ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg gcgataccac
     4981 gcgcaataca cagacgctgt tgctgaccac cagagagaga gtaaccgctc tggtgcaatt
     5041 tatctttggt ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca
     5101 tgtcggcacg ggagagcttc tcaaacagac gaacaccaaa agcgatgttg tcgtagatgg
     5161 acatcgggaa cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc agcagtgcga
     5221 tatcctgaga gttggtcagg atgttgtcgc catcaagcag aatttcacct tctgcgcgct
     5281 gctccggata cagttcaaac attttgttga aggtacgcag cagcgtcgat ttaccgcagc
     5341 cggacggccc gataaacgcc gttacctggt ttttagcgat atccaggttg atgtttttca
     5401 gggcatggaa tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag
     5461 tttcaaccat actcattgca atcttttcct catcgtacct gatgcttttc gctaatcagg
     5521 cttaccgtag gccgggtaag gcgttcacgc cgcatccggc aaagtgttaa ccgtgtttat
     5581 tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag gacgcacagg gtaatgatca
     5641 ataccccggc ccaggccagt tgttgccatt ccgcaaacgg gctcatcgca aacttaaaga
     5701 tcgtcaccgg caggttggcg atcggctgca tcatgtccgt gctccagaac tggttggaga
     5761 gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg ggcaatcgcc agcaggatac
     5821 cggtcataat cccggacacc gacgctttca gcgtaatcgc ggagatcatc ttccacttcg
     5881 gtgtacccag cgcataagcc gcttcacgca ggctgtccgg caccagtttc agcatgttct
     5941 cggtggtgcg gataacgatc ggcacctgca acagcgccag cgcaattacg cccgcccagc
     6001 cggagaagtg ctccatctgt gccaccacga tggtgtagac aaacagacca accacaatcg
     6061 acggcacgga gagcagaatg tcgttaatga agcgaatcac ttctgccagc caggatttac
     6121 gaccatattc cgccagataa atccccgcca taatgcccag cggcgtaccg aataccgtgg
     6181 cccacaaaat taacagcccg ctacccgcca gggcgttcgc cagaccacca ccttccgtat
     6241 tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat accgtcgata ccgcgagtga
     6301 tggtggacat taaaatccag atcagccaga acaggccgaa ggccatcgtc gccatcgaga
     6361 gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg cattttgcgg cgagattcag
     6421 ccagcgccgc agtggtttgc atttcaacca tagccattag cgtgccccct cattcttagc
     6481 caggcgcata atcataaact tcgatgcggc gaggacgatg aaggtaatca caaacaggat
     6541 caggcccagt tccatcagtg cggcaacgtg cagaccggat tccgcttccg caaattcgtt
     6601 cgccaacgca gaggtgatac tattgcccgg catgtacagc gaggcgctgt cgagctggta
     6661 ggtgttaccg atgataaagg tcaccgccat ggtttcaccg agcgcgcggc ccagccccag
     6721 catgatgccg ccgataacac catttttggt gaacggaaga acgatacgcc agataacttc
     6781 ccaggtggtg cagccaatac cgtaggccga ctctttcatc atcaccgggg tttgttcgaa
     6841 cacatcacgc attaccgccg caatgtacgg aataatcatg atggcgagga tcacgcctgc
     6901 cgcgaggata ccgataccaa atgcggggcc agagaacagc gcgccaacaa tcgggatatt
     6961 cgacatgata ttgccgaccg gctcctgaaa gtaaacggcg aacagcggcg caaagataaa
     7021 caggccccac atgccgtaaa cgatacttgg aatggctgcc agcagctcaa tggcgatacc
     7081 cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg aacagggcga taccgaaact
     7141 caccgggacg gcgatcagca gcgcgataaa cgaagtcacc aacgtaccgt agatcggcac
     7201 cagcgccccg tagatatcgt tcggtgcatc ccactctttg gtccatagga aagccagacc
     7261 aaatttctga atgctcggcc aggaggagat gatcagagag acaataatgc cacccaacat
     7321 caatagcaca atcagcgccg ccagttttac cagcacgctg aaaattatgt cgcccttttt
     7381 acccggtggg ttaaaagcag gcttggttgc agccataagt tactcttcag ttaaacgcgt
     7441 ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg cctggagttt
     7501 tattagtaca gcggcttacc gctactgtct ttaatattgg tcttccacgc agcgcgaacc
     7561 tgttcaacta cactatccgg caggctggcg taatccaggt cgttcgcctg tttagccccg
     7621 gttttgtacg cccagtcgaa gaatttcagc acttctgtgc cttgttctgg tttcttctga
     7681 tctttgtgga tcagaatgaa cgtggtagag gtaataggcc atgcatcttc gcctttctgg
     7741 ttggtcagat cctgagcgaa ggttttgctc cagtctgcac cttttgctgc attagcgaag
     7801 ttttcttcgg tcggactaac cggtttacca tcagcggaga tcagtttggt gtacgccagg
     7861 ttgttctgct tcgcgtaagc atattcaaca taaccaattg cacccggcag acgctgaacg
     7921 aacgcggcga taccgtcgtt acctttaccg cccagaccga tcggccattt tacggtagag
     7981 ccagtaccaa cgttgttttt ccactcttcg ttcactttcg ccaggtagct ggtgaagacg
     8041 aaggtagtcc cggagccatc tgcgcggcgt actacagcaa tgttttgtga aggcagtttc
     8101 agacccggat tcagtttggc gatggtttca tcatcccact tcttgatttt gcccaggtag
     8161 atgtcgccga gggttttacc atccagcacc agttcgccag acttcagccc tggaatgtta
     8221 accgccagca ccacgccgcc aatcacggtc gggaactgga acagaccttc ctgagccagt
     8281 ttttcgtcag acagcggcga gtcagaggca ccaaaatcaa cggtattagc gataatctgt
     8341 tttacgccac cggaagaacc gataccctgg tagttaactt tattaccgat ttctttctgg
     8401 taagtgtcag cccatttggc atacaccggc gcagggaagg ttgcacctgc acctgtcagg
     8461 cttgcttctg caaacacaga gaaagcgctc atcgataagg tcgcggcgac aacagttgcg
     8521 acggtggtac gcataacttt cataatgtct cctgggagga ttcataaagc attgtttgtt
     8581 ggctacgaga agcaaaatag gacaaacagg tgacagttat atgtaaggaa tatgacattt
     8641 ttatgacaga aaggaattaa caaataactt actgatttta aatgtgaaga tgaaaatccc
     8701 acatgtcatc tttttgtcat gatgcattta aaagtgataa agaaatgggt gagatgacaa
     8761 cgatgtgatg aacaatcgtt gtcagaccta ttagcgcaca atctcaacag ttatcgtcgc
     8821 tgttgcagta aattgccccg gggctgggcg agctccagta aggctcgcag gagcagctga
     8881 aaaagttaca ctacctgatt tttcaccata cactgttgat ttacccatgt ctgttggcaa
     8941 ctcaccaccg ttcactttga tttccttatt atttttgtcg taaattttta tccccacgtt
     9001 agagtttgaa gttacgacca ttgaattgtc cgtattgggt tcggtagcat aaaaagacgt
     9061 actgatatca tcggtattca gtatccccag gttttcacac tcaatagtga ctgttttagt
     9121 aattttatgt gttgttaccg cacttccaac aacagatgaa aaatctgcag ctggtatttc
     9181 tttaaaatca aaataaactg gctgtaaatt atttatttcg caactttgcg gagctgtaat
     9241 atcgccccga atataaacat cagccatcgg ctgtgagtaa tccaccgggg tgtcacggct
     9301 aattgttcca tacaatgtca ctatctttgt ttcaggaata atcaattttc ctgcaaatgt
     9361 cttcttgata taaaaatata ctatcgcttt acccccactg gatatactta aaggttcaat
     9421 gcggttgcaa ttgtactttg tattaccaga cgggttgtta gcatgatatt caaaaggtgc
     9481 attcacatac ccaaccccaa gaatatctat agcaatccca acatcaaggt tcggattaag
     9541 atcataataa ttaatattag agtgaacccc agcataagtt aatgcctggt ttatttttga
     9601 cgtataatag atatagggga attctcctac attaccagta tcacaatgac agcgtccagg
     9661 atatgaggat ccattagtta cagtggtatt aaatgtcgtt ccaaccttat tttcgttcgc
     9721 ggtaataaga tatcccgaga aatcaagcgt acggtgaaat acgccattat caggggtaca
     9781 gataccttta ggagctgcaa gtgctacact acttaacgag agcataaaca cagcaaagca
     9841 ccactgtttg atatacttgt tcataaaaat ccttttaatg acatgtcaca ccctgttcaa
     9901 ttaatccgct ctcactttct ggtgtcgaaa gtgaataatt gaagatgcat tttgttgagg
     9961 cgttttctcc ccaggataac gtaacctgtc ctttttctgg tagaccagaa agataaagtt
    10021 cgccacggtc accgacaatt cctgaatctg ccaaacttgc atcttgtatc gcagcaatgg
    10081 ctccaaaagg taccggttta ccatttgcat ttaacactct gaagagaacg cgataaccca
    10141 catgagtcac aaactccgct ttaaccactg caccacgcgt gggaaccatc gtaacgacgg
    10201 tattatcaag ttctatattc gacttcgaga aatagttatc gctgattgct acccgatttt
    10261 catcataagg gtttagctgt gtcttgaccg tatagccgcg ccagtccgtc gcagcaccgc
    10321 gcatattatc tatttctaac ccggcggccc caggtgcttt aacaagagca atagtttcac
    10381 ctaactcctg acttaacgtt accccttcgc tgtgtgccag aattccccct ctgataccat
    10441 aattcagcct ctgggaattt tggctgtagt tatagctgcc gttcaccgtg ccataagagc
    10501 catcataggt cagggacata ttaccgccaa cctcatcatc atgactgtta taggactgca
    10561 ttaaattcca tgaaaggtta tttctttcca gcgccgtacc gctgagtccg agttgatgtg
    10621 agctaccgga attatcgttt tgcgaaacag aataggtggc gtaactatta ccccaccagc
    10681 ccaataaagg aacagataaa gagagcatgt agatctgctc actggtgtta tcactggcat
    10741 ggttgttccc cgtcccctgt ctggcagtat tggaataact ccaggagacg ttccacgaga
    10801 gacgcttcca gctactgctg tagccaaatt gcattaatcg ttcgacatca tcggtatgcc
    10861 agtattgttg ccggcttaat gtcaggtata aattgccata atctctcccc aacgactgcg
    10921 ttagcgtccc ctccacgcaa ctacgtcggt tacctgtttc ccaaaagtct tcagggctgt
    10981 tgcgccgcga tgcccactca ttgagggtat agtagccctc ggtggagtaa cgatagccaa
    11041 ccaagcgcaa gctagtgtct gtgtcgtcaa atcgttttga atagagaaag cgatatgact
    11101 gacccgtttc tgtatcatca tggctaaaat tcgcacgagc atgtgtcaca tcgaaagaaa
    11161 ttgcgcccaa agcgccaaga tctttaccga cacccagagc tgcagaacca taattttcag
    11221 cagcctgcat acctccatat aaagtgatcc cgtgggaaaa accatgaagt atttgcgcct
    11281 gaaggacgtc cggtgtaaat ccgtcttcat cgcgcaattc acccacgctg acatcaacat
    11341 ctgtctgacc ttcacgttta agaatcgcta atgaagcata aggttgggtg aatcgttgtt
    11401 cgctaccgtc ttcttctttg atggttacg
//
LOCUS       DAJTVK010000127        11427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000127 DAJTVK010000000
VERSION     DAJTVK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.127"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO372_003438"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="OO372_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6342840.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO372_003439"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="OO372_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6342841.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO372_003440"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="OO372_003440"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6342842.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="OO372_003441"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="OO372_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6342843.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="OO372_003442"
     CDS             complement(3928..5214)
                     /locus_tag="OO372_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6342844.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="OO372_003443"
     CDS             complement(5235..6026)
                     /locus_tag="OO372_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6342845.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="OO372_003444"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="OO372_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6342846.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="OO372_003445"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="OO372_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6342847.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="OO372_003446"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="OO372_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6342848.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="OO372_003447"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="OO372_003447"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6342849.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="OO372_003448"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="OO372_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6342850.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="OO372_003449"
     CDS             complement(9882..10364)
                     /locus_tag="OO372_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6342851.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11427)
                     /gene="dgcN"
                     /locus_tag="OO372_003450"
     CDS             complement(10380..>11427)
                     /gene="dgcN"
                     /locus_tag="OO372_003450"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR6342852.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgct ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggcct tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtc
//
LOCUS       DAJTVK010000128        11358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000128 DAJTVK010000000
VERSION     DAJTVK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.128"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..538)
                     /gene="espL1"
                     /locus_tag="OO372_003451"
     CDS             complement(<1..538)
                     /gene="espL1"
                     /locus_tag="OO372_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR6342853.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQYA"
     gene            1063..2991
                     /gene="thrS"
                     /locus_tag="OO372_003452"
     CDS             1063..2991
                     /gene="thrS"
                     /locus_tag="OO372_003452"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6342854.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2995..3537
                     /gene="infC"
                     /locus_tag="OO372_003453"
     CDS             2995..3537
                     /gene="infC"
                     /locus_tag="OO372_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6342855.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3634..3831
                     /gene="rpmI"
                     /locus_tag="OO372_003454"
     CDS             3634..3831
                     /gene="rpmI"
                     /locus_tag="OO372_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6342856.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3884..4240
                     /gene="rplT"
                     /locus_tag="OO372_003455"
     CDS             3884..4240
                     /gene="rplT"
                     /locus_tag="OO372_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6342857.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4357..4481
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4363..4407
                     /gene="pheM"
                     /locus_tag="OO372_003456"
     CDS             4363..4407
                     /gene="pheM"
                     /locus_tag="OO372_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6342858.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4690..5673
                     /gene="pheS"
                     /locus_tag="OO372_003457"
     CDS             4690..5673
                     /gene="pheS"
                     /locus_tag="OO372_003457"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6342859.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5688..8075
                     /gene="pheT"
                     /locus_tag="OO372_003458"
     CDS             5688..8075
                     /gene="pheT"
                     /locus_tag="OO372_003458"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6342860.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8080..8379
                     /gene="ihfA"
                     /locus_tag="OO372_003459"
     CDS             8080..8379
                     /gene="ihfA"
                     /locus_tag="OO372_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6342861.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8480..9460
                     /gene="btuC"
                     /locus_tag="OO372_003460"
     CDS             8480..9460
                     /gene="btuC"
                     /locus_tag="OO372_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6342862.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9523..10074
                     /gene="btuE"
                     /locus_tag="OO372_003461"
     CDS             9523..10074
                     /gene="btuE"
                     /locus_tag="OO372_003461"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6342863.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10074..10823
                     /gene="btuD"
                     /locus_tag="OO372_003462"
     CDS             10074..10823
                     /gene="btuD"
                     /locus_tag="OO372_003462"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6342864.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10901..>11358
                     /locus_tag="OO372_003463"
     CDS             10901..>11358
                     /locus_tag="OO372_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6342865.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2872 a         2631 c         3059 g         2796 t
ORIGIN      
        1 gggcgtattg caatgacaga tggcgacaaa aaatagcgtc agaaggagat tgcgaaaaaa
       61 atgcactacc attgagattt actgttggtt gtatagtttt ttctctatgg atttttttag
      121 acagtttcat acaatggtta cgatcttcgg cagttaactg ttcctgttca atataatcca
      181 gtatgcttcg caaatggtta gccttaatag gcgtggtttc cagaaataag ggaatgattt
      241 cttttttata gtgtaatgta atgagtatat tttctgcatg gatgggtgaa gtggcaaagt
      301 taatgataaa aagggcagtg gaataacaat tttgtgaaat ttttcgacag tttgcggtaa
      361 attcctgagt aacacagtat gacagcatcg aacttaagtc agaggtatca agtagggtga
      421 tgagttggcg cgcaaattga ttaataggct ctgatgaagg cgttggtatg tcgctatcga
      481 tgtgcaatct atgaggagac ttataatggg ttctgataat gatatcgttt tgactcataa
      541 tctattcgcc ttatatatgt ctggaggttt tgaggatgat gattaattat tatcgccaga
      601 acgaaatgtg cacgaaaaat tgctataaga attgtgtcac ttttatcatt gtgatatgac
      661 cacttcgtca ttaatctcat gagcgtttaa aatactaccg ccaaatcacg caacgaactt
      721 tgctggattc agtttatgct gctgtaaatc cgctcgcagt aaacctttca gacgcaccgt
      781 gatgttatca gtggttcttt aagcgttttg ctggtgtact cactacaaac gaattgcgaa
      841 tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt ttagtgttaa catcgttcaa
      901 ccgggtggaa ggtgagaggt taatgtcttt catttgtgta cctgattgcg aaccaattta
      961 gcatttgttg gctagatggt ttcgcaatga actgttaata aacaaatttt tctttgtatg
     1021 tgatctttcg tgtgggtcac cactgcaaat taaggatata aaatgcctgt tataactctt
     1081 cctgatggca gccaacgcca ttacgaccac gctgtaagcc ccatggatgt tgcgctggac
     1141 attggtccag gtctggcgaa agcctgtatc gcagggcgcg ttaatggcga actggttgat
     1201 gcttgcgatc tgattgaaaa cgacgcacaa ctgtcgatca ttaccgccaa agacgaagaa
     1261 ggtctggaga tcattcgtca ctcctgtgcg cacctgttag ggcatgcgat taaacaactt
     1321 tggccgcata ccaaaatggc aatcggcccg gttattgaca acggttttta ttacgacgtt
     1381 gatcttgacc gcacgttaac ccaggaagat gtcgaagcac tcgagaagcg gatgcatgag
     1441 cttgctgaga aaaactacga cgtcattaag aagaaagtca gctggcacga agcgcgtgaa
     1501 actttcgcca accgtgggga gagctacaaa gtctccattc ttgacgaaaa catcgcccat
     1561 gatgacaagc caggtctgta cttccatgaa gaatatgtcg atatgtgccg cggtccgcac
     1621 gtaccgaaca tgcgtttctg ccatcatttc aaactaatga aaacggcagg ggcttactgg
     1681 cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg gtacggcgtg ggcagacaaa
     1741 aaagcactta acgcttacct gcagcgcctg gaagaagccg cgaaacgcga ccaccgtaaa
     1801 atcggtaaac agctcgacct gtaccatatg caggaagaag cgccgggtat ggtattctgg
     1861 cacaacgacg gctggaccat cttccgtgaa ctggaagtgt ttgttcgttc taaactgaaa
     1921 gagtaccagt atcaggaagt taaaggtccg ttcatgatgg accgtgtcct gtgggaaaaa
     1981 accggtcact gggacaacta caaagatgca atgttcacca catcttctga gaaccgtgaa
     2041 tactgcatta agccgatgaa ctgcccgggt cacgtacaaa ttttcaacca ggggctgaag
     2101 tcttatcgcg atctgccgct gcgtatggcc gagtttggta gctgccaccg taacgagccg
     2161 tcaggttcgc tgcatggcct gatgcgcgtg cgtggattta cccaggatga cgcgcatatc
     2221 ttctgtactg aagaacaaat tcgcgatgaa gttaacggat gtatccgttt agtctatgat
     2281 atgtacagca cttttggctt cgagaagatc gtcgtcaaac tctccactcg tcctgaaaaa
     2341 cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg acctggcggt tgcgctggaa
     2401 gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg ctttctacgg tccgaaaatt
     2461 gaatttaccc tgtatgactg cctcgatcgt gcatggcagt gcggtacagt acagctggac
     2521 ttctccttgc cgtctcgtct gagcgcctcc tatgtgggcg aagacaacga gcgtaaggta
     2581 ccggtaatga ttcaccgcgc aattctgggg tcgatggaac gtttcatcgg tatcctgacc
     2641 gaagaattcg ctggtttctt cccgacctgg cttgcgccag ttcaggttgt tatcatgaat
     2701 attaccgatt cacagtctga atacgttaac gaattgacgc aaaaactatc aaatgcgggc
     2761 attcgtgtta aagcagactt gagaaatgag aagattggct ttaaaatccg cgagcacact
     2821 ttgcgtcgcg tcccatatat gttggtctgt ggtgataaag aggtggaatc aggcaaagtt
     2881 gccgttcgca cccgccgtgg taaagacctg ggaagcatgg acgtaaatga agtgatcgag
     2941 aagctgcaac aagagattcg cagccgcagt cttaaacaat tggaggaata aggtattaaa
     3001 ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca atggcgaaat tcgcgcccag
     3061 gaagttcgct taacaggtct ggaaggcgag cagcttggta ttgtgagtct gagagaagct
     3121 ctggagaaag cagaagaagc cggagtagac ttagtcgaga tcagccctaa cgccgagccg
     3181 ccggtttgtc gtataatgga ttacggcaaa ttcctctatg aaaagagcaa gtcttctaag
     3241 gaacagaaga aaaagcaaaa agttatccag gttaaggaaa ttaaattccg tcctggtaca
     3301 gatgaaggcg actatcaggt aaaactccgc agcctgattc gctttctcga agagggtgat
     3361 aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg cgcaccagca aatcggtatg
     3421 gaagtgctta atcgcgtgaa agacgatttg caagaactgg cagtggtcga atccttccca
     3481 acgaagatcg aaggccgcca gatgatcatg gtgctcgctc ctaagaagaa acagtaaggc
     3541 cttcaagtag cactgtctgt ggagccttcg ggcttcacag gctttgttcg cctttgtttc
     3601 gtttattaac aatgcgaagt ggaagttatt aaaatgccaa aaattaagac cgtacgcggt
     3661 gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta agcacaagca cgctaacctg
     3721 cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc acctgcgtcc gaaagccatg
     3781 gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc cgtacgcata agccgttaac
     3841 gtttttaact ttttaattag aatatagata caggagagca catatggctc gcgtaaaacg
     3901 tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa caagctaaag gctactacgg
     3961 tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt atcaaagctg gtcagtatgc
     4021 ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa ctgtggattg cgcgtatcaa
     4081 cgcagcagca cgtcagaacg gtatttctta cagcaaattc atcaatggcc tgaaaaaagc
     4141 ctctgttgaa atcgaccgta agatcctggc tgatatcgca gtattcgaca aagtagcgtt
     4201 caccgctctg gttgaaaaag cgaaagcagc tctggcataa gccagttgaa agagggagct
     4261 agtctccctc ttttcgtttc aacgccatca aaacattgac ttttatcgcc gtagcctttt
     4321 caataaaggt cttttgaaga gtaccaaaag gtaacgcaag caatgaatgc tgctattttc
     4381 cgcttctttt tttactttag cacctgaatc caggaggcta gcgcgtgaga agagaaacgg
     4441 aaaacagcgc ctgaaagcct cccagtggag gctttttttg tatgcgcgtt ttgaaaaata
     4501 aaaagccagt ctatcaggcg taattgtcgc agccagtctg gacacggcca gcgcggagaa
     4561 accggagcgt atttaagtac gtgagaattt cgagcactgc ccgggaccaa aatggcaagt
     4621 aaaatagcct gatgggatag gctctaagtc caacgaacca gtgtcaccac tgacacaatg
     4681 aggaaaacca tgtcacatct cgcagaactg gttgccagtg cgaaggcggc cattagccag
     4741 gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa aaaagggcac
     4801 ttaacccttc agatgacgac cctgcgtgag ctgccgccag aagagcgtcc ggcagctggt
     4861 gcggttatca acgaagcgaa agagcaggtt cagcaggcgc tgaatgcgcg taaagcggaa
     4921 ctggaaagcg cagcgctgaa tgcgcgtctg gcggcggaaa cgattgatgt ctccctgcta
     4981 ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc gtaccatcga ccgtatcgaa
     5041 agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga agacgattat
     5101 cataacttcg atgctctgaa cattcctggt caccacccgg cgcgcgctga ccacgacact
     5161 ttctggtttg acactacccg cctgctgcgt acccagacct ctggcgtaca gatccgcacc
     5221 atgaaagccc agcagccacc gattcgtatc atcgcgcctg gccgcgttta tcgtaacgac
     5281 tacgaccaga ctcacacgcc gatgttccat cagatggaag gtctgattgt tgataccaac
     5341 atcagcttta ccaacctgaa aggcactctg cacgatttcc tgcgtaactt ctttgaggaa
     5401 gatttgcaga ttcgcttccg tccttcctac ttcccgttca ccgagccttc tgcagaagtt
     5461 gacgtcatgg gtaaaaacgg taaatggctg gaagtgctgg gttgcgggat ggtgcatccg
     5521 aacgtgctgc gtaacgttgg catcgacccg gaagtttact ctggtttcgc cttcgggatg
     5581 gggatggagc gtctgactat gttgcgttac ggcgtcaccg acctgcgttc attcttcgaa
     5641 aacgatctgc gtttcctcaa acagtttaaa taaggcagga atagattatg aaattcagtg
     5701 aactgtggtt acgcgaatgg gtgaacccgg cgattgatag cgatgcgctg gcgaatcaaa
     5761 tcactatggc gggcctggaa gttgacggtg tagaaccggt tgccggtagc ttccacggcg
     5821 tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa ctgcgtgtga
     5881 caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt ctgtggtgcg ccaaactgcc
     5941 gtcagggcct gcgtgtggcg gtagctacca ttggtgctgt tctgccgggt gatttcaaaa
     6001 ttaaagcggc gaaactgcgt ggcgaaccgt ctgaagggat gctgtgctcc ttctctgaac
     6061 tgggaatttc tgacgatcac agcggcatta tcgaactgcc tgcagatgcg ccgattggca
     6121 ctgacatccg cgaatacctg aaactcgatg acaacaccat cgaaatcagc gtaacgccaa
     6181 accgtgccga ctgcttaggt atcattggtg ttgcgcgtga cgttgccgtg ctgaatcagc
     6241 tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac catcgacgac acgttgccga
     6301 ttacagtcga agcgcctgaa gcctgcccgc gctatcttgg ccgtgtggta aaaggcatta
     6361 acgttaaagc gccaactccg ctgtggatga aagaaaaact gcgtcgttgc gggatccgtt
     6421 ctatcgatgc agttgttgac gtcaccaact atgtactgct cgaactgggc cagccgatgc
     6481 acgctttcga taaagatcgc attgaaggcg gcattgtggt gcggatggca aaagagggcg
     6541 aaacgctggt gctgcttgat ggcactgaag cgaagctgaa tgctgacact ctggtcatcg
     6601 ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg tggcgaacac tctggcgtga
     6661 atgacgaaac gcaaaacgtc ttgctggaat gcgctttctt cagcccgctg tctatcaccg
     6721 gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca ccgttatgag cgtggcgttg
     6781 atccggcact gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc gacatctgcg
     6841 gtggtgaggc tggtccggta attgatatca ccaacgaagc aacgctgccg aagcgtgcaa
     6901 ccatcacttt acgtcgtagc aaactggatc gcctgatcgg ccatcatatt gctgatgaac
     6961 aggtaactga cattctgcgt cgtctcggct gcgaagtgac cgaaggcaaa gacgagtggc
     7021 aggcagttgc gccgagctgg cgtttcgata tggagattga agaagatctg gttgaagaag
     7081 tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc ggtacaggca agcctgatta
     7141 tgggtactca ccgtgaagct gacctgtcgc tcaagcgcgt gaaaacgctg ctcaacgaca
     7201 aaggctatca ggaagtgatc acctacagct tcgttgatcc gaaagtgcag cagatgatcc
     7261 atccaggcgt tgaagcctta ctgctgccaa gcccgatctc tgttgaaatg tcagcaatgc
     7321 gtctttctct gtggaccggc ctgctggcaa ccgtggtgta caaccagaac cgtcagcaga
     7381 accgtgtgcg cattttcgaa agcggtctgc gtttcgtacc agatactcag gcaccgttgg
     7441 gcattcgtca ggatctgatg ttagccggtg tgatttgcgg taaccgttac gaagagcact
     7501 ggaacctggc aaaagagacc gttgatttct atgatttgaa aggcgatctt gaatccgttc
     7561 tcgacctgac cggtaaactg aatgaggttg agttccgtgc agaagcgaat ccggcactgc
     7621 atccggggca atccgcagcg atttatctga aaggggaacg tattggtttt gttggagttg
     7681 ttcatcctga actggaacgt aaactggatc ttaacggtcg cactctggtg ttcgaactgg
     7741 agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct cgcttcccgg
     7801 cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg gatattttat
     7861 ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt aaacttattt gacgtgtacc
     7921 gcggtaaggg tgttgcggag gggtataaga gcctcgccat aagcctgatc ctgcaagata
     7981 ccagccgtac actcgaagaa gaggagattg ccgctaccgt cgccaaatgt gtagaggcat
     8041 taaaagagcg attccaggca tcattgaggg attgaaccta tggcgcttac aaaagctgaa
     8101 atgtcagaat atctgtttga taagcttggg cttagcaagc gggatgccaa agaactggtt
     8161 gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt gaaactctct
     8221 ggttttggta acttcgatct gcgtgataag aatcaacgcc cgggacgtaa cccgaaaacg
     8281 ggcgaggata ttcccattac agcacggcgc gtggtgacct tcagacccgg gcagaagtta
     8341 aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac taaaaaggcc
     8401 gctctgcggc cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa cgccatgaca
     8461 cttcagcaga acggatacca tgctgacact tgcccgccaa caacagcgac aaaatattcg
     8521 ctggttatta tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc tttgcgccgg
     8581 tgaacaatgg atttcgccag gtgactggtt tactcctcgt ggcgaactgt tcgtctggca
     8641 aattcgcctg ccacgtacgc tggctgtatt gctggttggt gcggcgctgg ctatatccgg
     8701 cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa cctggactac ttggcgtctc
     8761 taacggcgca ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc aactccccaa
     8821 ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc atcactttaa tactcttacg
     8881 tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg gctggcgttg cattagggat
     8941 tatctgtagc gcactaatga cgtgggctat ctacttttcc acctctgttg atttacgtca
     9001 gctgatgtac tggatgatgg gcggttttgg cggcgtagac tggcggcaaa gctggctgat
     9061 gctggcattg atccccgtgt tgttgtggat ctgttgtcag tccaggccga tgaatatgtt
     9121 agcacttggc gagatctcgg cgcggcaact gggtttaccc ctgtggttct ggcgcaatgt
     9181 gctggtggca gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg gtgctatcgg
     9241 ctttattggt ctggtgatcc cacatattct tcggttgtgt ggtttaaccg atcatcgcgt
     9301 attacttccc ggctgcgcgc tggcaggggc gagcgcagtg ctgctggccg atattgtagc
     9361 gcgcctggca ttagctgccg cagagctgcc tattggcgtg gtcaccgcaa cgttgggtgc
     9421 gccggtgttt atctggttat tgttaaaagc aggacgttag ccgcaaaaag acggtctatg
     9481 attaaaagct aagattttta ctgaccacac ccaggagaaa cgatgcaaga ttccattctg
     9541 acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc tggagaagta cgccggtaat
     9601 gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata tgagcagttg
     9661 gagaatattc agaaagcctg ggccgatcga ggttttgtgg tgctgggatt cccgtgcaac
     9721 cagtttctgg aacaagaacc gggcagcgat gaagagatta aaacttactg taccaccaca
     9781 tggggggtga cgttcccgat gttcagtaag attgaagtta acggcgaagg acgccatccg
     9841 ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg cgccggaaga gagcggattc
     9901 tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc ctgatgatat tttatggaat
     9961 tttgagaaat ttttagttgg cagggacgga ttggtcatcc agcgtttttc cccggatatg
    10021 acgccggaaa accccattgt gatggaaagc attaaactgg cgttggcaaa ataatgtcta
    10081 ttgtgatgca gttacaggat gttgcggaat ctacccgcct agggccactt tctggcgagg
    10141 ttcgggctgg ggagatcctg cacctggtgg ggccgaatgg cgcgggtaag agtaccttac
    10201 tggcacgaat ggccggaatg accagcggta agggaagtat tcagttcgcg gggcaaccac
    10261 tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc ctatctttca caacagcaga
    10321 cgccgccgtt tgcaatgccg gtctggcact atctgacact gcatcagcac gataaaacgc
    10381 gtaccgaact actgaatgat gtcgcagggg cgctggctct tgatgacaaa ctcggacgta
    10441 gcaccaatca actttccggc ggtgaatggc aacgcgtacg tcttgctgcg gtggtgctgc
    10501 aaatcacacc acaagccaat cccgcaggcc aattgctgct tcttgatgag ccgatgaaca
    10561 gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct gagcgctctg tgtcagcaag
    10621 gactggcgat tgtgatgagc agtcacgatc tcaaccacac attgcgtcat gcgcatcggg
    10681 cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg cagagaagag gtgctcacgc
    10741 cgccaaatct ggcgcaggcc tatgggatga attttcgccg tctggatatc gaaggtcaca
    10801 gaatgctgat ttcgaccatc tgatttcatt agtatcttga aaatcgggta attacgacgc
    10861 taaattaatg caagaataaa aaacagagga accgtacgga atgcgtttct gccttatttt
    10921 gatcacagca ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc caaatgccag
    10981 actttctgat tcgattaccg ttattgccgg tttgaacgac cagctacaaa gctggcatgg
    11041 cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg gatttgtggt
    11101 tgtaacgatg cgcgatcgtt tcgatttgca gctgccccga gaaaccaaag aacaagcctc
    11161 tatcggcacg caaattgata aagacgagtt gctgcctggt gacctggtct ttttcaaaac
    11221 gggttccgga caaaatggtt tgcatgtagg tatttacgat accaacaacc aatttatcca
    11281 cgcctctacc agcaagggag tgatgcgttc ctcacttgat aatgtctatt ggcagaaaaa
    11341 tttctggcag gcgagacg
//
LOCUS       DAJTVK010000129        11338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000129 DAJTVK010000000
VERSION     DAJTVK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.129"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            24..236
                     /locus_tag="OO372_003464"
                     /pseudo
     CDS             24..236
                     /locus_tag="OO372_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            328..3012
                     /locus_tag="OO372_003465"
     CDS             328..3012
                     /locus_tag="OO372_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6342866.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3009..4097
                     /locus_tag="OO372_003466"
     CDS             3009..4097
                     /locus_tag="OO372_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6342867.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4180..5477)
                     /gene="exuT"
                     /locus_tag="OO372_003467"
                     /pseudo
     CDS             complement(4180..5477)
                     /gene="exuT"
                     /locus_tag="OO372_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5960..7372
                     /gene="uxaC"
                     /locus_tag="OO372_003468"
     CDS             5960..7372
                     /gene="uxaC"
                     /locus_tag="OO372_003468"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6342868.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7387..8874
                     /gene="uxaA"
                     /locus_tag="OO372_003469"
     CDS             7387..8874
                     /gene="uxaA"
                     /locus_tag="OO372_003469"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6342869.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8957..9508
                     /locus_tag="OO372_003470"
     CDS             8957..9508
                     /locus_tag="OO372_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6342870.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9513..10757)
                     /gene="sstT"
                     /locus_tag="OO372_003471"
     CDS             complement(9513..10757)
                     /gene="sstT"
                     /locus_tag="OO372_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6342871.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2978 a         2778 c         2942 g         2640 t
ORIGIN      
        1 agcggtatta agcgcagcta tttagcaaga ttgttccgct gacagtaacc tggggcggtg
       61 aagaaattaa agctgatgcg gcaaccactt ttaccgcaac caaaatcttt gcttccgatg
      121 cgttaactaa cggttctctg gctaaaaacc tgatgtttgc tcaaaccacc aaaggtgttc
      181 tggagacagg catctaccgt ggtgtagtaa gcatttatct gtctcaggat atctaatcca
      241 gataacagaa ttaacacgta acaaagggag gggtagccct cccttatttt aactgtttta
      301 gtatttgtta tggatttcag gattgccatg gataaaaaat tactggctct tttgatcctg
      361 gcgagtctca gcccggcaga ggcgacatta accgaaattc ccgcagggtt tgaggttatt
      421 gctcagggac agcaggagta tatcgaggtt tatttttcag ggaaaagtct cggtaaatat
      481 tatgcaatgg ttaatcttga taccgtaaca tttcttgatc cagcaagtct atataacaag
      541 ctggaactgg atgtagacga tcagaaaatc gcgcatatag tgaaagaaaa attatcgcag
      601 ccgctagctc accacggtga attggcttgc ggttatgtac gtactgactc agggtgtggt
      661 tttctgaata ccgatacgct ggaaataatc tataatgatg aagaaagttc ggcaacgttg
      721 tttattaatc cgcaatggaa ttcagctttc gatgcgaagt cattatattt aaatccagac
      781 aaaaatactg ttaatgcttt tatacatcag caagacatca atgttctggc acaggatgat
      841 taccaatcgt tgtctattca gggaaacggt gcgctgggaa taacagaaaa tagttatatt
      901 ggtgcacact ggaatttcaa cggttatgat gcagatgatg tcagtgacag taatgctgac
      961 gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt attatgtgca ggcgggtcgc
     1021 atggacaacc gcacactatt taatgcacaa ggcgggaact ttacctttaa ctttctgcca
     1081 ctcggtgcaa ttgatgggat gcgtatcggg tcgacactga gctatttaaa ccaggcgcaa
     1141 agccagcaag gaaccccagt aatggttctg ctttcgcgca attctcgtgt tgacgcttat
     1201 cgtaatgagc aacttttggg atcgttttat ctcaatagtg gttcgcaatt tattgatacc
     1261 agttcctttc cgccaggcag ctacagcgtg gcattaaaag tctacgaaaa taaccaactc
     1321 acccgcaccg agcttgtgcc gtttaccaaa accggcggtc tgactgacgg aaatgcgcaa
     1381 tggttcttac aggcaggtaa aactacatca caggtttctg atgatgaaag ttcagcttat
     1441 cagctaggag tacgcctgcc attacatccg caatatgagc tctacgcagg gctggcgaat
     1501 gccgatgatg tgagtgcttt cgagttaggt aataactgga cggcagattt aggcggggtg
     1561 gggaatcttg caatcagcgc cagcgtgttc cgtaacgatg acggcggcaa aggtgatatg
     1621 caacaggcca actggagtaa tccgggatgg ccgacgttgg gcttttatcg gaccaactct
     1681 gacggcgatg cttgtacaac cgacagcaga gagagctata acgccttaag ctgttatgaa
     1741 agtatttccg cgacggtttc acagaatttt gtcggctgga atatgatgct gggttatacc
     1801 cgcacacaaa ataacactga tgatagtttg cgttgggata aacagcagag ctttgaaaat
     1861 aactatcttc gccagacaac agcgcaaagt atctctgaaa ctgtacaact tagcgcttcc
     1921 cgcgcttttg tgatgcgtga ctggattttg agtacgtcag tcggtgtttt ccatcgtaat
     1981 gacaacggtg gcgataacga cgacaacggc ttgtacttat cgttttcgtt atctgacacg
     2041 ccaacgatgg atagcaataa caacagccat tcaacaaatg tgtctacgga ttatcgttat
     2101 agcgaacagg atggcgatca aacgtcatgg cagttatcgc atacctttta taacgattca
     2161 ttcagccata aagaacttgg cgtaaccgtc ggaggcctga acaccgatac cataaacagc
     2221 gcggttaacg ggcgttggga tgggcaatac ggaaatgtct acgctaccgt atctgatagt
     2281 tatgaccgta agaatcatga tcatctttcg gcctttacgg gcacttatag ctctacgctg
     2341 gctgtcagtt gctatggcgt taatttggga gccagtggta cagacgattt gctgggtgcg
     2401 gtactggtgg atgtgaaagg cttctctgaa caggatgaag agagtcagga tctgcaactc
     2461 gaagcgcggg tggcaggcag ccgaactttg cagcttggtc aaagtgacag tgtgttgttc
     2521 ccttatcctg gatttcagtc tggttttgtt gaggttaacg acagtagcca gggcaatcag
     2581 caggggacaa caaacatcat taacggtgca gggaatcgtg aattaatgtt gttgcctggc
     2641 aagctgcgct atcgcgaagt gtctgccagc tttaactaca actatatcgg tcgtttatta
     2701 ctgccggcag cggtgaaaaa attcccgatt gtggggctga acagcgccat gttactggta
     2761 gctgaagatg gaggatttac acttgaaatt aacggcagcg aaaaagagct ctatctgctt
     2821 tccgggcagc aattccttaa gtgtccactg agtgttgtaa agaaacgcgc cagcattcgt
     2881 tacagcggag atgttacttg tagtgtggtg acttattcac aattaccgga gtcgattcag
     2941 gttcaggcac agttgaaaca gcctaaatta cgtggaaacg ttcagacggc gcaaagggag
     3001 gttgcaccat gagaaatcga ttgattgcgg cgatattggg cttgtttggc acgctcactg
     3061 gcgttcaggc agctcctgac gtgaccagtg aaattacgta tgatttggca tctggtagag
     3121 cggattatta cttctggaag gatgaagcgt cggcaggaaa taatggatat atgtggtatg
     3181 aatgttcgta tcctgacctc caacaaacct gtacagctaa tggaaatata tcgacagtac
     3241 aaatctattt aactgaacaa cgcagtggga tgcgttggcc ggtaaaactc aaaggattta
     3301 aaacagccat tgtaagtagc gatgaagcgc cgccaggatg caaggggggc aaagggcttc
     3361 agacgaatct taaggattct aatagatctt catgtacaga agatggtcaa cattattata
     3421 tatacgatac aaagtttctt acgctctacc ttgagcagac agagatgaag aatttgccga
     3481 ttggtggcgt ctggaagggg aaagttaaat tacattcgaa cagcccggcc caggactatt
     3541 tcgcaaatat taccctgaat acgctcgacc ccaaccatat tgacgtgttc ttcccggagt
     3601 tcgcccacgc cacgccaagg gtgcagttag acttgcatcc aacaggaagc gttaacggca
     3661 gcaactacgc gcaagatctg accatgttgg acatgtgcct gtacgatggt tttaacggta
     3721 atgccatcag ttatgaaatc atgctcaaag atgaagggcg accagctgca gggcgcagag
     3781 acggtgactt ctctatctat cgtcaaggag ggaccaccac cgacgaggga gaacgcattg
     3841 attaccgggt caaaatgtac aacccggaaa ccggtgggca aattgatgtg cgcaataatg
     3901 aaaatatggt ctggaacagc attaacctga aacgtgtgcg tccggtggta ctgcccggta
     3961 ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct ggcagtcgat aaattcagcg
     4021 tgatggataa acaggccggg tactacatgg gcaaattgtc agtaatcttt acgccttcct
     4081 tgccaaccat caattgaccc cgcagaccag aaaaaactaa acaacgaagc cgcctgataa
     4141 ggcggcttcg tcattacaac ttacgttaaa cgttaaacgt taatgttgcg gtgcgggatc
     4201 gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc tgcaatacgg tccagataac
     4261 cagcgcaccc agcaggtcaa atactgccag aactgcgaac agcgggctga agccgatggt
     4321 gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt gccagccatg cggacatccc
     4381 ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat
     4441 cagcgcgcca gacagtgcct ggtgggcaaa accaccgata cacagcagca taattgcgac
     4501 atacgggttg gtgaacagac cgatcatacc cgggccaatc atcagcacag cacccagcgt
     4561 tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg
     4621 caggtaacca ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcgaacat
     4681 cgcgatctct ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt
     4741 aaaagtaccc caggccggtt ctgccaggaa acgcggcagt gcgatacccc agaactgacg
     4801 gttacgcagg atctgaccaa cggacatttt cttcgccgtg ctcacctggt gctgggcttc
     4861 ctgaccatta ataatatagt cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg
     4921 tttatagaaa atcagccatg ccatcgccca gataaagctc aatgcaccgg agatgataaa
     4981 tgccatctgc cagctgtgca tcacgattgc cataccacca gcggcggcgc aatcatcgca
     5041 ccaatcgaag aacctacgtt aaagtagcct actgcgatgg aacgctcttt cgccgggaac
     5101 cattcggagc tggctttcag acccgccggg atcatcgctg cttccgcggc accgaccgca
     5161 ccacgagcaa cagccaggcc accccagcta cctgccagcg cggttgcacc acagaacacg
     5221 gcccacagca cagcaaacat tgcataaccg attttcgtac ccagcacatc cagtacataa
     5281 cctgctaccg gttgcatgac cgtataagca gcagaatagg ctgcgatgat ataggaatac
     5341 tgttgggtgg agatgtttaa ctcttccatc agagttggcg cagctgccgc cacagtgtta
     5401 cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac
     5461 cctttaattt tacgcatgta aaacctcatc gttttgttat atccgcagga agcgcggcca
     5521 tcatcccgcc agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac
     5581 gccccgaacg ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat
     5641 tcatctaaca aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc
     5701 agaaagataa cttgtcatac aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt
     5761 tgtagggaca ttacctgacg acagcgaggc cagtactggc gcggcctgca gcgagattta
     5821 tcactttgag agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg
     5881 gactctggaa attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact
     5941 cgttgagagg aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga
     6001 atttgcccgc cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg
     6061 ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg
     6121 gctgaaaggc gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct
     6181 gtgtaccggt gatgcatctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca
     6241 cactatcggc aacccgttat accactggac gcaccttgaa ctgcgtcgtc cgtttggtat
     6301 cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact
     6361 gctggcgcag gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt
     6421 cggcaccacc gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg
     6481 ctctttcacc atcaaagtgc tgccgagctg gcgtccggac aaagccttca acatcgaaca
     6541 ggcgaccttt aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg
     6601 ctttgctgac ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg
     6661 taaagtgtct gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact
     6721 ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcaca
     6781 gttcaaaact gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca
     6841 gcagtaccac attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc
     6901 ggatgtaggc tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct
     6961 gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg
     7021 cgataacgaa gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa
     7081 aatgcagttc ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat
     7141 gacccaactg gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg
     7201 tagcttcctg tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg
     7261 tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa
     7321 aaatatttgc tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg
     7381 gttgatatgc aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat
     7441 ttggctgaag gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt
     7501 gctcgtggac ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat
     7561 ggcctgccga ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac
     7621 aatacgcgca cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat
     7681 ctgcctgcgc aagcggcaga tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc
     7741 ggggtgcgta acgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg
     7801 caaatccaga accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc
     7861 ctcttcagcc acacctacgg ctgctcacaa ctgggcgacg atcacatcaa catccgcacc
     7921 atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc
     7981 tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc
     8041 gttcatttca tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg
     8101 catcagctgt ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg
     8161 aagtttggtc tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg
     8221 ctggggcgtt tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg
     8281 ccggagatgt ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt
     8341 gaaaaactgg tcaccatggt caacgacttt aaacagtact ttattgccca tgatcagccg
     8401 atctacgaaa acccatcacc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa
     8461 tcacttggct gtacccagaa agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc
     8521 gagcgtctga aaacgcctgg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg
     8581 accagcgccc tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgcggcacg
     8641 ccatacggtg gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag
     8701 aaaaaacact ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag
     8761 ttgctggaag aatttatcga caccatcgtt gagtttgcca acggtaagca aacttgcaac
     8821 gagcgtaacg acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga
     8881 aatgagttgt taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac
     8941 ctgataagga tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt
     9001 tctgattggt atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc
     9061 ggtctatagc gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc
     9121 cagtgccatc cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt
     9181 aatggcgatt tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt
     9241 cgaactattg ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg
     9301 gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg
     9361 ggcggggtcg ataggcggca caatgattga gggtagtttt ctgcttatga atggcttgaa
     9421 tatcattcgt ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac
     9481 ccctcccgcc gtagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga
     9541 cgatcgtctt ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc
     9601 gcggtttcgc aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg
     9661 gcgatatcgt tcgaaatacc gaacatatta caggccagcg ggatcagcag cagagaaccc
     9721 cccgccacgc cggatgcgcc acaggcacac agagaagcca ccacgctcaa cagcagcgcc
     9781 gtgggcagat cgaccgggat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg
     9841 gtgattgctg cgcccgccat attgatggtg gcacccagcg gaatagaaac ggaataggta
     9901 tcgcgatcca gattcagctt ttcacacagt gccatattca ccggaatgtt cgccgcagag
     9961 ctgcgggtga agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac
    10021 gggttacgac gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac
    10081 atacagccaa ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg
    10141 gttgccaggg tagaagaaac cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt
    10201 ttcaccataa aggtgacggc attcgacata tcgttgacca ggtttttggt ggtctcgtta
    10261 ccgtgacgca gtgcgaaacc gaggccaatt gcccacacca ggatcccgat gtagttacct
    10321 ttcagcagcg cgtcgatggg gttggaaacc atgctcatta ccagcccgcg catcacttct
    10381 acaatgcctg acggtggcga aatatcaccc gcgctactgg ataagtgcag ggtagaaggg
    10441 aaggcgaagc tgaaaacgac tgcggccaga gcagcagaga aggtacccag cagatagagg
    10501 aacaaaatag ggcggatatt ggttttctgc ccatgctggt ggttagcaat agatgccatc
    10561 accagcatca acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct
    10621 aacagaccaa cagcttccgc cgcgggtttt gatatccatg ccagaagaat ccccagaaca
    10681 aggccgacca ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt
    10741 gaacgttgcg tagtcatttt tcgatccttt cattgtgtta tcgcacatcc ctggtgcgtt
    10801 ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt
    10861 acagcttcgg gaagcgaaaa atgctggatt ttaaagagaa gtcatatttt gaaacttaat
    10921 ggttacattt gtttgacata aacagaggga aggactggct actaaggctg attagataac
    10981 atatgggcaa aactatttgg aatatgagaa cggttgcgct cctttaatgt gctatgccat
    11041 ataaagtcac tggcgcaggg agcgcgcagg gggcggccaa tcgccgccgc cccctgcacc
    11101 cccaggctct ggcgaacaaa atcgccgctg cgcgattccc tcagcttatc ccttgcggct
    11161 accgggtcgg gcgcaaggga acgtccctgt aagatgcgcc ctcgccgcgc atccatgcgc
    11221 gtcgccccgg cctccaggga acgcttcggc gattttgacg ccaccaacac ccgtgcggtc
    11281 tttttgtaaa gagctacaag ccgattacat aattaatagc tcacctttgt ctggaatg
//
LOCUS       DAJTVK010000130        11211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000130 DAJTVK010000000
VERSION     DAJTVK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.130"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(270..1043)
                     /locus_tag="OO372_003472"
     CDS             complement(270..1043)
                     /locus_tag="OO372_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR6342872.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="OO372_003473"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="OO372_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HCR6342873.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="OO372_003474"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="OO372_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HCR6342874.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /locus_tag="OO372_003475"
     CDS             1926..2666
                     /locus_tag="OO372_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6342875.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="OO372_003476"
     CDS             complement(2637..3404)
                     /locus_tag="OO372_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6342876.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OO372_003477"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="OO372_003477"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6342877.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="OO372_003478"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="OO372_003478"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6342878.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OO372_003479"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="OO372_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6342879.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="OO372_003480"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="OO372_003480"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6342880.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..9490)
                     /locus_tag="OO372_003481"
     CDS             complement(8354..9490)
                     /locus_tag="OO372_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6342881.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(9959..10336)
                     /locus_tag="OO372_003482"
     CDS             complement(9959..10336)
                     /locus_tag="OO372_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342882.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            complement(10336..10614)
                     /locus_tag="OO372_003483"
     CDS             complement(10336..10614)
                     /locus_tag="OO372_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6342883.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            complement(10784..>11211)
                     /locus_tag="OO372_003484"
     CDS             complement(10784..>11211)
                     /locus_tag="OO372_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6342884.1"
                     /translation="HIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTV
                     RYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHI
                     AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         2789 a         2777 c         2665 g         2980 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt
     8581 gccagtggtt tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat
     8641 aatatctgac cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga
     8701 ctgccacgca taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag
     8761 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactgattg
     8821 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
     8881 tattaagccg cccctggttt ccttttaccg cgaataaata atcaccgccc tgtttttgta
     8941 tcttctctgc aatatctttc tggcatccca tcgcatcagt tgtgatgatt tttcctttaa
     9001 tatccagcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
     9061 taatctgccc gagaaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc
     9121 ccttgcggcg actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt
     9181 ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg tgaaattttg
     9241 caggactgat acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa
     9301 aatcaccata ttgcttcaaa aaatcgagat gtgtttcacc aaaatcctct atatcttccc
     9361 aaccctctgc accagaaatg acggcacaaa tagtcaacag tagaatatct gacaatttat
     9421 gttctacttt ccaggcttgt ctgtaatcgg ggataataga aatatgttcc atcagttttt
     9481 taagttccat tttgttctcc ttatttatgc aaggagtatt tgatcatata taagctatga
     9541 aaaatagttt taggtaataa ggaatatctc aattattgaa catttagcgc gaattattta
     9601 gtacaaaaag tgtacttccg atgatctttc cctgctccaa agtcttctaa aatcaacaaa
     9661 gtagactctt aattatattt atcaacacaa tagtctatcc tattactatc ttgacaagag
     9721 agaaccgttt tttatttaaa tatatgatac acatcattaa atatataacc atactttttc
     9781 ataaggcaat ctaacttagc tcttaaatca ccaaagccca tcacaccata acatctaata
     9841 gccattgcca cacgtcacta tggatttatt tccacctgca attacttcgt cctaacttta
     9901 atttaatctc aagaagcatt aagatctcaa tagcgagctt aatacttctt gaataatatc
     9961 actcctgacc aaaatttttc cacactaatg catgagactt tgatttgtca cccagaaatt
    10021 tacttaaatc ttgccaaagt aaagaattgc tatcggtcgc agtgactatt gcctctataa
    10081 gccctgtagc tacaactgtg gccaattcat catcaagcga gaccatacca tcttcgacat
    10141 gtttaaatat tttttctctt tcaatatccc ccatttcata aaaatttttt acaatcgttt
    10201 ttccaacctg agagaacaac ataaccaccg gtggcgtttc tggatgccag tattcattag
    10261 ctaatgagac tgtctcagac aaaacagtgg aaaatgatgc cattttttct atataactag
    10321 caatatattg gttcattact tgcctcccaa agcattctgt aaatcctgaa gaacgttttc
    10381 tgctgctgct cgatcccctg gagatgctgt tggattctta tccaaccaat cctgtaaagc
    10441 ccttgtataa tcctgtgctt tcaatacatg tcctctacca ccaacttttt cacctgtggc
    10501 caattcataa cgaacagcat ctgctgtgct tccacttcct acttttgcat ttggccgata
    10561 aagatcgtct ataatatttt tcaaatttga atctgtaacg tcgggttttc ccattttctg
    10621 tcgcaattca tctttatacc gttcatgctg catacgatcc cttgcgctat tctcatcttc
    10681 tggtccccat cctcccgggc ttcccgtact gccgccggcc aagcagttct tctcttcatc
    10741 cgtcaaatcc ttcccaacat tcggctgact ccctagggaa agattacgaa agcccgctcc
    10801 ccgcaaggac tgacgcgaga tagtttctat ccatggctgc ttttcgcatc ttacgtctta
    10861 accctgcctt gaatacctta tcattcgtta aaatattaat agcgatgtgc cgtatccctg
    10921 aaaataattc tgcggcgttt cctcttctta ttttgcagtc gtcttcattc attaccacgt
    10981 ccagacgcca gtgcagctta ttctccacgt gccagtggtt tcggattgct gtggcgaact
    11041 tttctgcggt taaatcagca gaactgatat aatatctgac cgtcatttct ggctctttct
    11101 tttgttctgc tattattgac cgaaaggaga ctgccacgca taatttcttc agtcctttcc
    11161 attcaaacgt gaaatcaata agttcatcag ggacatcgca aacaatatga a
//
LOCUS       DAJTVK010000131        11151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000131 DAJTVK010000000
VERSION     DAJTVK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.131"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1224
                     /gene="narU"
                     /locus_tag="OO372_003485"
     CDS             <1..1224
                     /gene="narU"
                     /locus_tag="OO372_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6342885.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1306..5046
                     /gene="narZ"
                     /locus_tag="OO372_003486"
     CDS             1306..5046
                     /gene="narZ"
                     /locus_tag="OO372_003486"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCR6342886.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5043..6587
                     /gene="narH"
                     /locus_tag="OO372_003487"
     CDS             5043..6587
                     /gene="narH"
                     /locus_tag="OO372_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6342887.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6587..7282
                     /gene="narW"
                     /locus_tag="OO372_003488"
     CDS             6587..7282
                     /gene="narW"
                     /locus_tag="OO372_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6342888.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7279..7959
                     /gene="narI"
                     /locus_tag="OO372_003489"
     CDS             7279..7959
                     /gene="narI"
                     /locus_tag="OO372_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6342889.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8038..8931
                     /locus_tag="OO372_003490"
     CDS             8038..8931
                     /locus_tag="OO372_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCR6342890.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="OO372_003491"
     CDS             complement(9027..9872)
                     /gene="nhoA"
                     /locus_tag="OO372_003491"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCR6342891.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10045..10614
                     /locus_tag="OO372_003492"
     CDS             10045..10614
                     /locus_tag="OO372_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCR6342892.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="OO372_003493"
     CDS             complement(10618..10845)
                     /gene="pptA"
                     /locus_tag="OO372_003493"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCR6342893.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10945..>11151)
                     /locus_tag="OO372_003494"
     CDS             complement(10945..>11151)
                     /locus_tag="OO372_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6342894.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2592 a         2912 c         2977 g         2670 t
ORIGIN      
        1 gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta
       61 accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct
      121 atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat tccttgcgtc
      181 tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct
      241 ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc
      301 tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt
      361 gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc
      421 ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg
      481 atttgggtac cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc
      541 gccagttcac gcgcctcaat tgacgaccag ctccctgtct tacaacgcct gcatctctgg
      601 ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt
      661 gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc
      721 ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc
      781 gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt
      841 accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg
      901 tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc
      961 cagataacca tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagaaagaa
     1021 gccatcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc agtgggcggc
     1081 ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg
     1141 atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt
     1201 cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg
     1261 acaaggcatc agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg
     1321 gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg
     1381 atgcatagca accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatt
     1441 gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa
     1501 aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg ccctgaccag
     1561 cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctctacagc
     1621 gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgtgaagct
     1681 ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag
     1741 tgcttaagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa
     1801 cttaaccaac tgattgccgc cgctaacgtc tggaccatca aaatctatgg tccggatcgc
     1861 gttgccggtt tctcgccgat cccggcgatg tcgatggtgt catacgctgc cggaacgcgt
     1921 tatctgtcgc tgttaggcgg cacctgttta agcttctacg actggtattg cgacctgccg
     1981 cccgcctcgc cgatgacctg gggcgaacaa accgacgtgc cggaatctgc cgactggtat
     2041 aactccagtt acatcatcgc ctgggggtct aacgtaccgc agacacgtac gccggacgcc
     2101 cacttcttta ccgaagtacg ttacaaaggc accaaaacta tcgccattac ccctgactac
     2161 tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc
     2221 ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa tcccagcgac
     2281 tactttatca actactgccg ccgctatagc gacatgccga tgctggtaat gctggaacct
     2341 cgcgacgatg gtagctacgt acccgggcgc atgatccgcg cgtctgacct ggtagatgga
     2401 ctaggcgaaa gcaataatcc gcagtggaaa accgtggcgg ttaataccgc aggtgaattg
     2461 gtagtgccga atggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa
     2521 tccattgccg ccggtaagga aaccgaattg tcgttaaccc tgctcggtca acatgacgct
     2581 gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta
     2641 aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc
     2701 aacacctgtc cggtggtcag cgtttatgat ttggtgctgg cgaattacgg cctcgatcgc
     2761 gggctggaag atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc
     2821 tggggtgagc aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt
     2881 gccgataccg cccataaaac gcatggacgc tcaatgatta tcctcggcgc tggcgtcaac
     2941 cactggtatc acatggatat gaactaccgt gggatgatca atatgctgat cttctgcggt
     3001 tgtgtcgggc aaagcggcgg cggctgggcg cactatgtcg gtcaggaaaa actgcgccca
     3061 caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgcccacc gcgccagatg
     3121 aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct
     3181 caggagttac tttcaccgct tgccgatgcc agtaagtaca gcggtcatct gattgatttc
     3241 aacgttcgcg ccgaacgcat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg
     3301 ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag
     3361 gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat
     3421 ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag
     3481 tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc
     3541 agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc
     3601 gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtt
     3661 ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat gcatccgttt
     3721 attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga ctgggaaatc
     3781 tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg caaagaaacc
     3841 gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca gccgtgtgaa
     3901 gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc gccgaatatt
     3961 gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg
     4021 atggacaaac ttggcaatgg tggtaaaggg atttcgtgga atacccagga tgaaatcgat
     4081 ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt
     4141 gacaccgcca ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat
     4201 gttgcagtca aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg
     4261 gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa
     4321 attatctcca gccccacatg gtccggtctg gaaagcgatc acgtctccta taatgcggga
     4381 tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat
     4441 caggatcatc cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc
     4501 gacacccgta gcgtcagtga gatgcgccag atcccgccaa acggcttccc ggaaaaagca
     4561 cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg
     4621 ctaatgctga cgctctctcg cggtggaccg attgtctgga tcagcgaaac cgatgcccga
     4681 gaactgacca ttgtcgataa cgactgggtg gaagtcttta acgccaacgg cgcgctgacg
     4741 gctcgtgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta tcacgcccag
     4801 gaacgcatta tgaatattcc tggttcggaa gtaactggca tgcgcggcgg cattcataac
     4861 tcggtcaccc gtgtttgccc gaaaccgacg catatgattg gcggttacgc gcagctggcc
     4921 tggggcttta actactacgg caccgtcggc tcgaaccgcg acgaattcat catgatccgc
     4981 aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca ggaggcgaaa
     5041 aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgacaaatgt atcggttgcc
     5101 atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg gagtacgcat
     5161 ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg gaagatcagg
     5221 aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg cgtctgggca
     5281 gcaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag attgatgatt
     5341 actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa ggcaaacata
     5401 tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa gtgatctggg
     5461 ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc gaccgcaact
     5521 tcgaggccat gcaaaaggag atgtacgggc agtttaaaaa caccttcatg atgtacctgc
     5581 cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc gacctgccca agcggcgcta
     5641 tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc cgtggctggc
     5701 gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa agcggcaagt
     5761 cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg ccaacccacc gtgtgctcag
     5821 aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc gaccgcattg
     5881 aggaagcggc gagcaccgag cgcgaagttg acctctatga acgtcagtgc gaagtgttcc
     5941 tcgatccaca cgatccctca gtgatcgagg aagccctgaa acaaggtatt ccacaaaacg
     6001 tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg aaactggcgc
     6061 taccgctgca ccctgaatat cgcaccctgc caatggtctg gtacgttcct ccgctgtcgc
     6121 cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag cgaaggcgtg ctgcccgcca
     6181 tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc ggcgataccg
     6241 gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg cgttcacaaa
     6301 ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc gtcgcccagg
     6361 tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt gttatcccga
     6421 ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc ggttttacct
     6481 ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt agccgtatcg
     6541 atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc agatcctcaa
     6601 agtgatcggc ctgttgatgg agtatccgga tgagctgttg tgggaatgca aggaggacgc
     6661 gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca acctgcttaa
     6721 cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg ggcgcaccac
     6781 gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc aggcaatggt
     6841 ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg aactgcccga
     6901 ttatctaccg ctgtatctgg agtatttaag cgtactgcct gacgatcagg cgaaagaagg
     6961 attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac aacgcgaggc
     7021 accgtggtac gcgttgtttg atgctctgct gcaactggcg ggaagtatac tttcaagtga
     7081 cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg cgcttgatgc
     7141 agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt gtgacagctc
     7201 gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt atgtcgacat
     7261 cagtgcggga ggtgggaaat gattcagtat ctgaacgtct ttttttacga tatctacccg
     7321 tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta cgggcagtac
     7381 acctggcgcg cctcctcaag tcaaatgctc gataaacgcg ggatggtgat atggtcgaat
     7441 ctgttccata tcggcatttt ggggattttc ttcgggcact tgttcggcat gttaacgccg
     7501 cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactcatggc gatggtgctc
     7561 ggcggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg gcgcagtctg
     7621 acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat gagcattttg
     7681 ctgatccagt gtctgttggg gctaagtacc ataccgtttt cagcacagta tcctgatggt
     7741 agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg tggtggatcg
     7801 tcagagatgc tcaacggcgt agcgttcgtc ttccgcctgc atctggtgtt gggaatgacc
     7861 atcttcctgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcgcc gtttgagtac
     7921 tttactcgtc gatatcaaat tgtgcgttcg cggcgataat ttcaccctgg ccggatagcc
     7981 cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag aacgttaatg
     8041 aaaccgcagg tgtaccacgt cgatgctttt acctctcaac catttcgcgg caattctgcc
     8101 ggtgttgttt tccccgccga taatcttagc gaagcacaaa tgcagcttat cgcccgcgag
     8161 ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt gcgcatccgc
     8221 tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc tgcgcactat
     8281 gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc gctggcagga
     8341 aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct ggaacaaggt
     8401 acgccgggct ttgaactgcc gctggagggt gaagtacgcg cggcgattat caacgcattg
     8461 catcttactg aagacgatat tctgccgggt ttgccgatcc aggtcgcgac gaccgggcat
     8521 tctaaagtga tgatcccatt gaagccggaa gtggatatcg acgctctttc gcctgacctt
     8581 aatgcgctga ccgctatcag taaacagatt ggctgcaatg gtttcttccc gttccagatc
     8641 cgtccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg tattgtggaa
     8701 gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca tcacaacgta
     8761 ttgccccacg atggcaacgt gttgtgtgtt aaaggccatc aggggcgcgc attggggcgt
     8821 gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa agtcaccatt
     8881 tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaaatctg aaatcgttgc
     8941 ctgatgcgct tcgcctgtca ggtctacaac cgggctaatg tatgccggat aaggcgttta
     9001 cgccgcatcc ggcaacctga cataaattat tttcccgcct ccggatgcgt atcaaacgcc
     9061 gccatcacca gcgctaactc atccacagta aagccatgtt tcgcatcatc cacgcccaga
     9121 ccaaactgtt cctgcatcac ggcatataac gacgccacat ccggtagatt tcgctgctcc
     9181 accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagtgtcag cttgccgccg
     9241 tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg ccagtgcgcc
     9301 gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc gaagcggtac
     9361 atcggctgcc aatgctgatg atgattaaac tgcaacaccc aatcatcacc ctcctgcaac
     9421 aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg aatcggcgcg
     9481 gttagcgtct gcccaccgaa accgacatca gcaatccatt tttccccttc cagttccacc
     9541 aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac tacgcgccct
     9601 aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc gctcaaacac gccattctgc
     9661 tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga ttgatcatca
     9721 agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt gcaattgtgt
     9781 ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga ccagttaata
     9841 cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg gttttctcaa
     9901 cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac atgcatcacg
     9961 tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact acgcttaaaa
    10021 aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt acaccatgcc
    10081 agccgtctgt taaatcatgg cccaactgtc ttgatcacca gtttcgatga gcaatcccag
    10141 cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc tccacgcgtg
    10201 gcaattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa cggtaagttt
    10261 ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcggtggg aagtgtgtcg
    10321 gtgcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg tccggtgctt
    10381 ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct gctacctgcg
    10441 acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc agcggcagac
    10501 gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa tacgttgcat
    10561 catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg ttaagcatta
    10621 catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggctcgattt cggcatccca
    10681 gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg agctatcttt
    10741 actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg ctttttgttg
    10801 ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta cgctcctttt
    10861 gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta aagcgtttat
    10921 acgacaataa tgcccagggc aagattacga aagcccgctc cccgtaagga ctgacgccag
    10981 gtagtttctg cccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    11041 atcattcgtc aaaatattaa tagcaatgtg ccgtatccct gaaaataatt ctgctgcatt
    11101 tcctcttctt attttgcagt cgtcttcatt cattaccacg tccagacgcc a
//
LOCUS       DAJTVK010000132        11103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000132 DAJTVK010000000
VERSION     DAJTVK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.132"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..309
                     /locus_tag="OO372_003495"
     CDS             <1..309
                     /locus_tag="OO372_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6342895.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OO372_003496"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OO372_003496"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6342896.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO372_003497"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO372_003497"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6342897.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="OO372_003498"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="OO372_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6342898.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OO372_003499"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="OO372_003499"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6342899.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OO372_003500"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="OO372_003500"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6342900.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OO372_003501"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="OO372_003501"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6342901.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OO372_003502"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="OO372_003502"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6342902.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /locus_tag="OO372_003503"
     CDS             complement(8117..9763)
                     /locus_tag="OO372_003503"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6342903.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /locus_tag="OO372_003504"
     CDS             complement(9766..9816)
                     /locus_tag="OO372_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342904.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OO372_003505"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="OO372_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6342905.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10354..11103
                     /locus_tag="OO372_003506"
                     /pseudo
     CDS             10354..11103
                     /locus_tag="OO372_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2522 a         3055 c         2909 g         2617 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctatttt tatgatagct ttaataattt caatagaagt tgttgttttt taaaaagaaa
    10141 atagtgagtg aaaaaaattc aatttcagta taaatgttgt tcccttcctc gtagttcatc
    10201 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
    10261 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
    10321 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
    10381 gcagccctgg gagtaaatgc gccccctgat cactgttgag gtacatatca gtaaaggtct
    10441 acccggctta acgatggtgg gcttaccaga aacaactgta aaagaagctc gcgatcgcgt
    10501 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10561 tgctccagcc gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10621 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatgaat tagtcggaga
    10681 actggctctt acaggcgctc tgcgtggcgt tcccggtgca atctccagtg caactgaagc
    10741 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10801 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10861 taagcacgct ctcgaacgcc cgaaaccaac tgatgcagta tcccgggcgc tacaacatga
    10921 tctcagtgat gttgtcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10981 caggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    11041 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagcg ctgcgatatt
    11101 aag
//
LOCUS       DAJTVK010000133        11001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000133 DAJTVK010000000
VERSION     DAJTVK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.133"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..278)
                     /locus_tag="OO372_003507"
     CDS             complement(<1..278)
                     /locus_tag="OO372_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6342906.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(445..1185)
                     /locus_tag="OO372_003508"
     CDS             complement(445..1185)
                     /locus_tag="OO372_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6342907.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1278..1913)
                     /gene="argO"
                     /locus_tag="OO372_003509"
     CDS             complement(1278..1913)
                     /gene="argO"
                     /locus_tag="OO372_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6342908.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2052..2912)
                     /gene="mscS"
                     /locus_tag="OO372_003510"
     CDS             complement(2052..2912)
                     /gene="mscS"
                     /locus_tag="OO372_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6342909.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="OO372_003511"
     CDS             complement(3270..4349)
                     /gene="fbaA"
                     /locus_tag="OO372_003511"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6342910.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="OO372_003512"
     CDS             complement(4564..5727)
                     /gene="pgk"
                     /locus_tag="OO372_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6342911.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5777..6796)
                     /gene="epd"
                     /locus_tag="OO372_003513"
     CDS             complement(5777..6796)
                     /gene="epd"
                     /locus_tag="OO372_003513"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6342912.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7168..7599
                     /locus_tag="OO372_003514"
     CDS             7168..7599
                     /locus_tag="OO372_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6342913.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7622..8200
                     /locus_tag="OO372_003515"
     CDS             7622..8200
                     /locus_tag="OO372_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6342914.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8201..8908
                     /locus_tag="OO372_003516"
     CDS             8201..8908
                     /locus_tag="OO372_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6342915.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8896..9573
                     /locus_tag="OO372_003517"
     CDS             8896..9573
                     /locus_tag="OO372_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6342916.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9567..10244
                     /locus_tag="OO372_003518"
     CDS             9567..10244
                     /locus_tag="OO372_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6342917.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10216..10929)
                     /locus_tag="OO372_003519"
     CDS             complement(10216..10929)
                     /locus_tag="OO372_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6342918.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10926..>11001)
                     /locus_tag="OO372_003520"
     CDS             complement(10926..>11001)
                     /locus_tag="OO372_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6342919.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2626 a         2920 c         2741 g         2714 t
ORIGIN      
        1 cgtgaacgac gccctgagtt atggatttca ttatcaatcg cagaaagtcg ttgataaacc
       61 gggaacagtt tttgatacag ttcctgaccg gctttattga ggctgatatt attatctttg
      121 cgggtaaata gcgtatagcc aatttgttct tccagcgcgg caatgctttt accaaatggc
      181 gaagccgtca tatggatttt ttctgccgcc cgggcaatat tattggtttg cgctaataaa
      241 atgaaattgc gcatcttttt cgaaataaaa atgtccatcc cccctccccg catccttgtc
      301 cgcttaaccg tttcagtcag cctatccttg atgaaaccgc gagcaaagat aggtgattac
      361 gtcatggttt tacagaaaat tacagaaaaa ggaggcaata tcgggtaaag gcattagccc
      421 gacgaatacg tcgggctaca agtattattg tgctgcaggt gttttagcgg gttgttgatc
      481 cacaggttct aactggaaga ccacatcgac ctgatcatca aactgaatag cggcctgctc
      541 gtaagtttcc tgggcagaca ccggcgcggc atcggctttc atcatccgca ccattgggct
      601 gggctgatag ttggaaacat ggtagcgcac gctatatacc ggccccagtt tacgatgaaa
      661 gccgttcgcc agttcctgcg cctgatgaat cgcgttatca atcgctgcct tacgcgcttt
      721 gtctttatag gcatccggct gcgccacgcc cagcgacaca gaacgaattt cgttaagacc
      781 cgccttcagc gcgccatcca gcaaggaatt cagtttgtct aactgacgga gcgtgacttc
      841 caccgttctc acagcgcggt agcctttcag gatactttta ccatcctgat aatcataatc
      901 tggctgggtg cgtaagttcg ctgagctgat atcttttttc gcgatctgat tgagttcaag
      961 gaaggaaatg tattgtgcga cgcgctcatc tgcctgtttc ttggctgtag cggcatcctt
     1021 cgcggccacg ttaacttcaa tcgcaagagt ggcaatgtct ggcaccgcat ccacacttgc
     1081 cgtaccggag gtgacaatat gcggtccatc cggcaattcg ttagcctgcg ctgccatccc
     1141 gctaataccc attaatgccg ccagggcgat aactttgaac ttcacgttag tctctccgtg
     1201 ttaattgact tgcccaaaat tcaggacctc tggaggcaag cttagcgcct ctgttttatt
     1261 tttccatcag atagcgccta actgaacaag gcttgtgcat gagcaatacc gtctctcgcc
     1321 agctgcaagg ctataaacca catgacacat cccactacca gattgataat gcgctgtgat
     1381 tttgccgtgc gcaggcgcgg tgccagccag gctgcgagaa tagccagacc aaagaaccac
     1441 aggaaagagg cgctaattgt cccgagcgca aaccaacgtt ttggttcgac gtcaagctgc
     1501 ccgccaaggc tgcccagtac aacaaaagta tccaggtaaa catgcggatt cagccaggtc
     1561 actgccagca tggtggcgat aattttccat ctgccttgct tcaggacttc ggcactagct
     1621 aactcaatat tactactcat cgctgtttta aaagcgccaa aaccatacca cagcaagaag
     1681 gctacgccgc cccaggtgac cagcgccagc aaccacggcg actgcatcaa taacgcgctg
     1741 ccaccaaaaa tcccggcgca aatcaggacc aaatcgctga tagcacaaag taaggcaatc
     1801 ataatgtggt actgacgacg aatgccctga ttcatcacaa aagcattttg tggaccgagc
     1861 ggcagaatca tagctgcccc aagagcaata ccttgaaagt aataagaaaa cacgttggat
     1921 attccgaatt aagtatctga tgcagagact atatcgcggg attgttatta gcggaaattg
     1981 ataatattaa ttggcaatca gaataactaa taagagagag gccagagagt attatctggc
     2041 ctcagcgttg attacgcagc tttgtcttct ttcacccgct taaagttcac atccatttgc
     2101 gggtacggga agctgatacc ggcggcatca aattcacgtt taatacgctc cagcacatcc
     2161 cagtacacgt tttgcagatc gccgctgttg ctccagacgc ggaccacgaa attaatcgac
     2221 gatgcaccaa gttcgttcag acgcacagtc atttcgcgat ctttcaaaat gcgatcttca
     2281 gactggataa tattggtcag gatctgctta acctgatcga tatcggaatc atacgccacg
     2341 ccaataataa attcgttacg gcgaactggc tcgcgagaga agttaataat atttccggca
     2401 ataattttac cgttcggaat aacgataatt ttaccgtctg cagtacgcat ggtggtggag
     2461 aaaatctgca cactcagcac agtaccggct acgccgccga gatcaacata ttctccggca
     2521 cggaacgggc ggaacatgac aagtaacacg ccagcggcca ggttagaaag tgacccctgc
     2581 aaagccagac caacagctaa gcctgcggca ccgagtacag caatgactga cgctgtttgt
     2641 acacccacgc gtcccagggc agcgattagc gtaaaggcga taataccgta acggactaat
     2701 gcagaaagaa aatcagcaac agtggcatcg attttacggg agatcatcag gcgattcacc
     2761 gcgttggaaa tcatccgcgc gataatcaaa ccaacgatga tgatcgcgag tgccgccacg
     2821 atgtttactg cataacttag cagcagcgcc tggttagcta ccagccagct tcccgcgccg
     2881 tttatgctat cgacaacatt caaatcttcc attcaatatt ccttttcaac tgactcaaaa
     2941 tggagaaata cactccgccg ccttatgacg ggcagtctga caagggtaaa caataaagca
     3001 taattttgcc aaatagatca cagatttctc atctgaatca ccttgctttt ggcgaaaaaa
     3061 tccccgcaga gcggggctca gacagatatc aatgtgcgct ttgtcatgcc ggatgcggcg
     3121 tgaacgcctt atccggccta caaaaacctg caaattcaat atattgcagg atacgtgtag
     3181 gcctgataag cgtagcgcat caggcaattt tgcttttggc gaaaaaaaga cccgcagagc
     3241 gggccttgag ataagcagaa aggaatatct tacagaacgt cgatcgcgtt cagttcctgg
     3301 aatgcttttt ccagacgagc gatcatcgaa gtctgaccgg cacgcagcca tacgcgcgga
     3361 tcgtagtatt tcttgttcgg ctgatcttcg cctttcgggt tacccaactg cccctgcaga
     3421 taagcttcgt tcgctttgta gtagttcaga acgccttccc aggttgccca ttgggtatcg
     3481 gtatcgatgt tcatttttac tacgccgtag cttacggagt ctttgatttc ctgagcagta
     3541 gaaccggaac caccgtggaa tacgaagttc aggctgttgt gcggcaggtt gtgtttcttg
     3601 gaaacatatt cctgagaatc acgcaggatg gtcggagtca gaaccacgtt acccggcttg
     3661 taaacaccgt gtacgttacc gaaggacgca gcgatggtga aacgcgggct gattttgctc
     3721 agttcggtgt atgcgtaatc aacgtcttcc ggctgggtgt acagtgcaga agcgtccatg
     3781 tggctgttgt ccacgccgtc ttcttcacca ccggtgcaac ccagttcgat ttccagagtc
     3841 atgccgattt tggacatgcg ctccaggtat ttagagcaga tttcgatgtt ctcttgcaga
     3901 gattcttcag acaggtcgat catgtgagaa gagaacagcg gcttaccggt agctgcgaag
     3961 tgtttttcac ccgcgtccag cagaccgtcg atccacggca gcagtttctt cgcgcagtgg
     4021 tcagtgtgca ggataaccgg aacaccataa tgttcagcca tctggtgaac gtgatgcgca
     4081 ccagagatcg cgcccaggat agcagcaccc tgcggaacgt cagatttcac gcctttacca
     4141 gcgataaagg aagcaccacc gttggagaac tgaacgataa ccggcgcttt aactttagca
     4201 gcggtttcca gtacggcgtt gatggagtca gtaccgacgc agtttactgc tggcagtgcg
     4261 aagttgtttt cttttgctac ctggaaaact ttctgtacgt catcaccagt gattacgcca
     4321 ggttttacga aatcaaaaat cttagacatg tctcttgtcc tgtatcgtcg ggccttatac
     4381 tcatcatact tcaagttgca tgtgctgcgt ctgcgttcgc tcaccccagt cacttactta
     4441 tgtaagctcc tggggattca ctctcttgtc gccttcctgc aactcgaatt atttagagta
     4501 tgaaaaaatg cgtgctctaa aagcgcgctg aaacaagggc aggtttccct gccctgtgat
     4561 tttttacttc ttagcgcgct cttcgagcat cgctactgca ggcagtactt taccttccac
     4621 gaattcgagg aatgcgccgc cgccagtgga gatgtaggag attttgtcag caatgccgaa
     4681 caggtcgatt gctgccagag tgtcgccgcc gccagcgatg gagaacgctt cgctgtctgc
     4741 gatagcgtta gccacgattt cagtaccttt gcggaagttc gggaattcga acacgccaac
     4801 cggaccgttc cacagaatgg ttttcgcatt cttcaggatt tcagccagtt cctgagcgga
     4861 agcatcaccg atatccagga tctgctcgtc agctttcaca tcgttaacag atttcagggt
     4921 agccgatgca gtttcagaga actcggttgc tacgcgaaca tcagacggaa ccgggatgtt
     4981 gcaggtggtc agcagacgtt tagcttcgtc aaccaggtca gcttcgtaca gggatttacc
     5041 cacatcgtgg ccttgtgccg cgataaaggt gttagcgata ccaccaccaa caatcagctg
     5101 gtcagcgatt ttagacaggg agtccagaac ggtcagtttg gtagatactt tagaaccacc
     5161 aacgatagcc accatcgggc gagcaggttc tttcagtgct ttacccagcg cgtccagttc
     5221 agctgccaac agcgggcctg cgcacgcaac atcagcgaat ttaccgatac cgtgagtaga
     5281 agcctgcgcg cggtgagcag taccgaatgc gtccattacg aacacgtcac acagtgcagc
     5341 gtattttttg gacagggttt cgtcgtcttt cttctcgcct ttgttgaagc gaacgttttc
     5401 cagaacaacc agttcacctt cagcaacgtc aacgccgtcg aggtaatctt taaccagacg
     5461 aaccgggtta gacagtttgt ctttcaggta gttaacaacc ggcagcagag agaattcttc
     5521 gttgtactcg ccttcggtag gacgacccag gtgggaagtt accatcactt tcgcgccttg
     5581 tttcagggcc agttcgatgg tcggcagaga agcacggata cgcgcgtcgc tggttacttt
     5641 cccgtctttt actggtacgt tcagatccgc acggataaat acacgtttcc cagcaagatc
     5701 cagatcggtc atcttaatta cagacatggt gaatcctctc gttgattcta aaagttttgc
     5761 agacgctgct tgcgtcttac ctgaaagcaa cagtagccat agctaacgtc gtgtcgagca
     5821 ttcggttagc aaagccccat tcgttatcgc accagaccaa cgttttgatc aggtgtgcgc
     5881 cactgacccg ggtttgggtg ccatcgacaa tggcactgtg cggatcgtgg ttaaaatcta
     5941 cagagaccaa cggcaattcc gtatagtcaa ctataccatg aaatgcacct tgtgctgctt
     6001 tttgcagcaa caggttgact tcattggctt ttacaggttt cttcaccgtc acgcttaaat
     6061 cgattgccgt cacatttatg gttggcacac gtaccgcaat cgcttcaaag cgatcgttaa
     6121 attgcggaaa aaatcgtgtg ataccggcgg ccagtttagt atcgaccgga atgatcgact
     6181 ggctggctgc ccgggtgcga cgcaggtcag gatggtatgc atcaataacc tgttgatcgt
     6241 gcatggcgga gtgaattgtg gtcacagtgc cggactcaat accgtacgca tcatctaaca
     6301 atttgatgac gggaattatg caattcgtgg tacaggaagc gttagaaacg atgcggtgtt
     6361 ccgcacgaag ttgatcctga ttgacgccgt aaacaacggt cgcgtcgaga tcgttactgc
     6421 caggatgtga aaagagcact tttttggccc cggcggcaat atgcgcttcg ccatgctcgc
     6481 gggagccata tacgccggtg cagtcgagga ctacatcaac gccaagttca cgccagggga
     6541 gcgattgcag tgaacgttca tgcaatacgc ggatggcgtc atcaccaaca aaaagttgat
     6601 cgcgttcctg tcgtacttcc catgcaaaac ggccatggct ggtgtcatat ttcaacaaat
     6661 gcgccatgcc cgcagcatcc gccagttcgt tgattgccac cacggtaatt tccgcccggc
     6721 gtccggattc atacaaagca cgaaccacat tacgcccgat gcgaccgaag ccatttatcg
     6781 ctacgcgtac ggtcatagat ctcctgcaag gttttccctg agcaaatttg ccagacagag
     6841 taatccagca aatcgtccgg ggaaacctta cctgtcgcaa actgcgactg attggttaat
     6901 tgtcgaacat ttaatcgact gaaacgcttc agctaggata agcgaaacgt ggaataaaag
     6961 gaatgattgt ccagtcaaag aaaacaatta tctgacttac gtcacgtttt cagccccgcc
     7021 cctgtggaat ttattccact ttgcagaatc acgaataagc gtagcaaaat cttttcgttg
     7081 tgccgtcaca aaccctgact tatatcagaa tatgactgat gcagcgtcga tattatccgc
     7141 aatacgtccg agtttcggag tactccaatg atgacattgc cctacccata ttcttccccc
     7201 gcacgctttt atgcactgcg cctcctcccc gggcaggaag ttttttccca gctacacgct
     7261 tttgcacagc agcatcaact tcatgccgcc tggattgcag gctgtaccgg tagcctgact
     7321 gacgtcgcct tgcgctatgc cgggcaagaa ggcaccacgc tccttaacgg tacattcgaa
     7381 gtgatctcac taaacggcac gctggaacag agtggtgagc atcttcatct ctgcgtttcc
     7441 gatccacacg gcacgatgct tggcggtcat atgatgcccg gctgtaccgt gcggaccacg
     7501 cttgaactgg tgatcggtag ccttgaggaa ctggcgttta gccgccaacc ttgcgcgctt
     7561 tctggttacg acgagttgca tatttccccc gttaaataac tactttcaaa gaggttttct
     7621 tatggcgcgt agccactttt ccagtcaggc actggtgttg atcgttatat ctatcgccat
     7681 caatatgatc ggcgggcaac tggccagcat ggtcaaatta cctatatttc tcgactccat
     7741 cggcacactt attagcgcgg tactgcttgg tccggtgatc ggaatgctga ccggcttact
     7801 gaccaattta ctctggggat tgctgaccga cccgatcgcc gccgcatttg cacccgtcgc
     7861 gatggtcatc ggcctggtcg ccggttggct ggcgcgagcg ggttggtttc gcaccctgcc
     7921 aaaagtcgtt gtcagcggcg tgattattac gcttgcggta acggtcgtcg ctgtaccgct
     7981 gcgtactgcg ctgttcggcg gtgtcaccgg tagcggagcc gatttgttcg ttgcctggat
     8041 gcactcgatg gggcaaaacc tggtggagtc ggtggcgatt acagtgatag gcgcgaacct
     8101 ggtcgataaa attcttacag cggtgattgt ctggctttta ctgcgccagc tgccgattcg
     8161 caccacgcgc cattttccgg caatggctgc cgtgcgctaa atgcatccat ttacctcatt
     8221 aacactgtgg gcattggcgg cctgcaccac gcttatcctg cccgcgcaga ccattttgcc
     8281 gatctatagc gcggcgacct ttttttgtct tattgcgcta aaggccacgc ggcggcgggc
     8341 aaaatatgtc gcctggctga tgttctcgct gggagccggg ctgtggctgg tacacggtgg
     8401 ctggctaaca gaatggctaa gcggcacgcc gcgttcgccg gagcgttgga cgcatgctat
     8461 tacgctctgg ttgcggattc tggcgattgt ttcaacctca cagttgtgga tgcagtacgt
     8521 ccccgtgcaa tgttttatcc gcgctctgtt tgcttctcgc ctgccgccag gcgtcgctta
     8581 tctgtttgcc ggaccgttat tggtcgttga gcagttaaaa cggcaattag cgattattca
     8641 tgaagcgcag cgtgcccgcg gcgtgccgct ggatgagggc tggtatcagc gtttacgtgc
     8701 gatgcccgcg ttaattattc tgctaacaca caatgcgctt aacgatctgg ctgtccgtgg
     8761 tgcggcgctg gatatgcggg catttcggat ccacaaccga cgaacaacgt tgtgggcacc
     8821 agccgatagc accctacaac gcgtagcgcg ttacaccatg atcttgctga tgctaacaga
     8881 attcggagcg tggatatggt tacgttagaa cagttccgtt attgcccgac acactcaacg
     8941 catcctccgt tctgttatga ctttcactat gtcaaaccgg ggatggtcgc catctttggt
     9001 gacaacggca gtggcaaaag tacgctggca cagctgatgg cgggctggta tccggacttt
     9061 ctgccaggag agataacagg cacggggacg ctcctcggta cgcccattgg gcatctcccg
     9121 cttaatgaac aatccgccac catccagctc gtccagcaat cgccctattt gcagctctcc
     9181 ggctgtacct ttagcgtgga agaagaagtt gcctttgggc cagaaaatct gtgtcttgct
     9241 gaaacagaga ttatggcgcg gattgatgcc gccctgactc tgaccgagtg ccagccgttg
     9301 cgccatcgtc acccggcaac actctccggt ggagagacac aacgcgtggt catcgcctgc
     9361 gccatcgcga tgcagccaaa gctcttaatt cttgatgaag cctttagtcg cctgacgccg
     9421 caggccagcg aaatgctgtt gcagcgctta caacactggg cgcttgaacg tggttcgctg
     9481 attattcttt ttgaacgtca tcgcacacct ttcctgaact actgccagca agcgtggcaa
     9541 ttgcaaaacg gagcgctcca gccactatgt taacgttaaa tcagatatct tatcgctggc
     9601 ctagcgcagc aacggattgt ctttgcgaca tttctctgca actcaaacaa ggtgaatggt
     9661 tggcgctgac cggcgacaat ggcgcgggaa aatcgacgct gttacgagta atggcaggtc
     9721 ttcttacgcc cacagccggt acggttatgc tgcaacaaca ggcgatgaaa aacctgaaaa
     9781 accgccagcg cgcggcaaag gttggcgtac tgtttcagga agcggaaaac caactttttc
     9841 acagcaccgt cgctgacgag atcgcatttg gtttgaaact gcaaaaatgc ccggcggatg
     9901 aaatcactca acgtacccat gctgccctgc aatgttgtca gttagcagac actgccaact
     9961 cgcacccgtt ggatctgcat agtgcgcagc gacggatggt cgccgtcgcc tgcctggaag
    10021 cactctctcc gccactatta ctgctggacg agccgagtcg ggattttgac gaaaattggt
    10081 tgagcgtgtt tgaaagctgg ctggagaagt gccgccagcg aggcacgagc gtggtagcaa
    10141 tcagtcatga cgccgcattt acccggcgtc atttctctcg tgtggtgcga ctggaagatg
    10201 gtgtaatcag gaacgttaat ccaccagacg atattcaccc gtagcggtca tcattaaggt
    10261 caaatttgca ggcagactct cttccagcac ccggcgaaca ttcggcccgt cggtacggtc
    10321 ataaaaggct tctgcatctg ccagcgatac ccctcccgcc agcttgcgcc ccaccagacg
    10381 ctcccgcaac gcagtagctg gcgcgttgat aaagatcgag aaatcacaaa attgcgcaag
    10441 ctggcaccac ttctcgtcgt ccagcaacaa ccagtttcct tcgacgatga cgagtggtgc
    10501 ggtaacgtgc aacgcatctt caacaggatc atgcttttgt cgatcgtact gcggccacgt
    10561 acaatcccct tccaccacct ggcgcagatt ttccgccagt ttcgcaacgt caaatgtctc
    10621 tggtgcgcct ttgaaggggc gcaattgatg cgcgtccagc cagctattgt aatggtgaaa
    10681 accatccatc gggagcgtct gaatagcggg cagctccggg tcttgttgca cgagatattc
    10741 ccagaaggtg gttagggtag atttacccgt ccccggcgga gcgcaaagaa aaaccaccgt
    10801 acgccgctgc ggatttaccg tctgtaaggc cgccagcata cgcagcaacg gtttatgcac
    10861 gcgttctatc tcttcatcat ggtactgcgc ttgcacgttc agcccattca ccgttaattc
    10921 aattttcacg gcgttaattc ctttaaggtg gtcgttactg ccagcgtcag cgccgttttg
    10981 accattccgc taaaacgcgc c
//
LOCUS       DAJTVK010000134        10894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000134 DAJTVK010000000
VERSION     DAJTVK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.134"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..611
                     /locus_tag="OO372_003521"
                     /pseudo
     CDS             1..611
                     /locus_tag="OO372_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="OO372_003522"
     CDS             608..1222
                     /locus_tag="OO372_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR6342920.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO372_003523"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="OO372_003523"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6342921.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="OO372_003524"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="OO372_003524"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6342922.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="OO372_003525"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="OO372_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6342923.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="OO372_003526"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="OO372_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6342924.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO372_003527"
     CDS             complement(5289..6338)
                     /gene="phoE"
                     /locus_tag="OO372_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6342925.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            6626..7729
                     /gene="proB"
                     /locus_tag="OO372_003528"
     CDS             6626..7729
                     /gene="proB"
                     /locus_tag="OO372_003528"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6342926.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7741..8994
                     /gene="proA"
                     /locus_tag="OO372_003529"
     CDS             7741..8994
                     /gene="proA"
                     /locus_tag="OO372_003529"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6342927.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9109..9184
                     /locus_tag="OO372_003530"
     tRNA            9109..9184
                     /locus_tag="OO372_003530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9142..9144,aa:Thr,seq:cgt)
     gene            9790..9948
                     /locus_tag="OO372_003531"
     CDS             9790..9948
                     /locus_tag="OO372_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342928.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2760 a         2574 c         2777 g         2783 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcataacttt gccatagaca
     6241 tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc agatgccaca
     6301 atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga ttttaattaa
     6361 cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa cctgagattg
     6421 ttaaagatat attacagatt aataatattc ttaaaatgtg gtaatttatt aaatctgtaa
     6481 taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac gccatgcttt
     6541 gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg atatcatttt
     6601 tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg taaaactcgg
     6661 caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatattg ttgaacttgt
     6721 tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga cgtcgggcgc
     6781 gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta tcgcctcgaa
     6841 acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac agctgttttc
     6901 gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg aagaccgtga
     6961 acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata tcgttccggt
     7021 aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata acgataacct
     7081 ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga ccgatcaaaa
     7141 aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta aagatgttta
     7201 cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc tcggaactgg
     7261 cggcatgagt accaaattgc aggccgctga cgtggcttgc cgtgcgggta tcgacaccat
     7321 tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca tttccgtcgg
     7381 tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga ttttcggtgc
     7441 gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc tggaacgcgg
     7501 cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc gtggtgaagt
     7561 catccgcatt tgcaacctcg aaggccgcga tatcgcccac ggcgtcagtc gttacaacag
     7621 cgatgcatta cgccgtattg ccggacacca ctcgcaagaa attgatgcaa tactgggata
     7681 tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag gagctggccg
     7741 atgctggaac aaatgggcat tgctgcgaag caagcctcgt ataaattagc gcaactctcc
     7801 agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc acaaagcgaa
     7861 atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gagccaatgg ccttagcgaa
     7921 gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc cgacgatgta
     7981 cgtcaggtgt gcaacctcgc cgatccggtg gggcaggtaa tcgatggcgg cgtactggac
     8041 agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt gatttatgaa
     8101 gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg taatgcggtg
     8161 atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc ggtgattcag
     8221 gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga taatcctgac
     8281 cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct gatcccgcgt
     8341 ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt gatcacaggt
     8401 ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga agcattaaaa
     8461 gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga aacgttgctg
     8521 gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat ggcggaaagc
     8581 ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg ccctgcgaag
     8641 gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga tttgaacgtc
     8701 aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg cacacaacac
     8761 tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa cgaagtggat
     8821 tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca gtttggtctg
     8881 ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat ggggctggaa
     8941 gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc gtaaataaaa
     9001 ccgggtgatg caaaagtagc catttgattc ataaggccat tgacgcatcg cccggttagt
     9061 tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc cgatatagct
     9121 cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc tattatcggc
     9181 accattaaga aaattaataa cttagcttaa cttctctaag aaattgtatc ttctgttatc
     9241 acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat gggcgagtag
     9301 ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc accgtctcta
     9361 aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc caatgatgct
     9421 acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag ttttaagtgc
     9481 aatagtggat taaagcccac ctcttcaaaa cctaacttga tcgggaaaga atcaagctct
     9541 agcacggttt cgtccaccgg agtacaacgg gtgcctgata agagaagaac tgctaagact
     9601 tcatagcttt tgtgaaaaag ccctttttag tggatacgcc tacggcgctt attttttggt
     9661 ataaccatca aaggcgcttt atggctccta ttgtcttgaa aatgtatctt tacttgtatt
     9721 gtgttttatg cgtagtctcc ctgtgtagta tgtaacttgt taattttcat atgaatacag
     9781 aggcacacga tgaaaaacat tgcagcaatc aaacgtaata accgcaagat tcacgctcgt
     9841 aagttcctgt ctacgccaga aggaaaagcc tggctagagc gtaaacagag agagaacgaa
     9901 gaaagaaaac tccttagtga gttgaaatgg cttagagagg atttctgaaa atctccagaa
     9961 cgcaacagga cgcattctaa cgctgttttc attactcggt atagggatag gtgatgtgtg
    10021 tttaatgcgt ctgtgcgctt gttttgtgcc ttattttgca ttttcataat gcggaagaga
    10081 acacggaaaa ccttaaaatt aattattggg gtatttaagg ctattttcag gtgatttaag
    10141 ggtattaaac ttgatttaat tgattaaaat ataaacagat aattatgtat cttattgatt
    10201 tataaggtta tttttaagta aaggtaaata aaaaataaat agccataaat agctataaat
    10261 acatcttaat acctatatcc cttcccttat ggctagattg cgagaggtgc ttaacgcgat
    10321 tttcagcgcc ccccgatctg gaaaatccag aatccccaaa tatcagcaag gaattttctt
    10381 gcgtaatcaa catcgttttt acctattaaa tcattacaca gaaagccttt ctgtaatgtc
    10441 tctcactaaa ctttcactaa acacgagaat gatcggtttt atgtcggatc agatgctaga
    10501 aacagcacca cgtctcacaa gggctgtaag cgatgaaacc agtgtttatg cgggtgcagg
    10561 tcaaaatata ggccaaaatc catttaacat aataaatgtt atatgtgcta aggaacacat
    10621 ataacgcctt gaattgatgt atcagtggaa aagtgatgga ttttttacgt aggagcacga
    10681 aagggcttct attgactttt gtgtgctatg aaatataatt aaagtttttt gtttttattg
    10741 tgtgatgtgg tgggttacac aggggaatag attaaatggt gttctgttta tacaggttta
    10801 tttgttagat caacaatttg gcatttctaa acagatgttt gaacgttgcc aaatgtcttg
    10861 acaacacaag aaaagggctt gctatgccag gcat
//
LOCUS       DAJTVK010000135        10843 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000135 DAJTVK010000000
VERSION     DAJTVK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.135"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO372_003532"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="OO372_003532"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6342929.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO372_003533"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="OO372_003533"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6342930.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="OO372_003534"
     CDS             complement(2698..3390)
                     /locus_tag="OO372_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCR6342931.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /locus_tag="OO372_003535"
     CDS             3520..5280
                     /locus_tag="OO372_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6342932.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5686..6543
                     /gene="focA"
                     /locus_tag="OO372_003536"
     CDS             5686..6543
                     /gene="focA"
                     /locus_tag="OO372_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6342933.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6598..8880
                     /gene="pflB"
                     /locus_tag="OO372_003537"
     CDS             6598..8880
                     /gene="pflB"
                     /locus_tag="OO372_003537"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6342934.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9072..9812
                     /gene="pflA"
                     /locus_tag="OO372_003538"
     CDS             9072..9812
                     /gene="pflA"
                     /locus_tag="OO372_003538"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6342935.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(9905..10081)
                     /locus_tag="OO372_003539"
     CDS             complement(9905..10081)
                     /locus_tag="OO372_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR6342936.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            10181..>10843
                     /locus_tag="OO372_003540"
     CDS             10181..>10843
                     /locus_tag="OO372_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6342937.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     ILD"
BASE COUNT         2792 a         2712 c         2567 g         2772 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc attaataaaa taattgtaat tataaggtta
     5401 aatatcggta atttgtattt aataaatacg atcgatattg ttactttatt cgcctgatgc
     5461 tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca tttgttaatt
     5521 tttaaaattt atttttattt tgataatcaa atgtttactc cgtatctgca taaaaaccat
     5581 gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct catctataat
     5641 gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa agctgacaac
     5701 ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc gggtgtctat
     5761 aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg tgttttcatc
     5821 tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat gcccttcggc
     5881 atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg tgttgtctgc
     5941 ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag tgggcgcatc
     6001 acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct ggtcggcgca
     6061 ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa tggtcaatgg
     6121 ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat tgaggccgtc
     6181 tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag ttattctggc
     6241 cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt tgttgccagc
     6301 ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt aatccgcgac
     6361 ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt ttctcacctg
     6421 accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa cattatcggt
     6481 ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa cgaccaccat
     6541 taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag gtgttacatg
     6601 tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg tgactggcag
     6661 aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga gggtgacgag
     6721 tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt aatggaaggc
     6781 gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt tgcttccacc
     6841 atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt tggtctgcag
     6901 actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat gatcgaaggt
     6961 tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt cactgaatac
     7021 cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct gcgttgccgt
     7081 aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat catcggtgac
     7141 taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact ggcacagttc
     7201 acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat ccgtctgcgc
     7261 gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc tgcgaaatac
     7321 ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg gacttacttc
     7381 ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg tcgtacctcc
     7441 accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac cgaacaagaa
     7501 gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt cctgcgtact
     7561 ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc tatcggtggt
     7621 atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct gaacaccctg
     7681 tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga aaaactgccg
     7741 ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct gcagtatgag
     7801 aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc ttgctgcgta
     7861 agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa cctggcgaaa
     7921 accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca ggttggtccg
     7981 aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga gcgcatggat
     8041 cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat ccactacatg
     8101 cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga cgttatccgc
     8161 accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc tgcaatcaaa
     8221 tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt cgaaatcgaa
     8281 ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc tgttgacctg
     8341 gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc tatcccgact
     8401 cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg taacacccca
     8461 gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca cggtcgtgac
     8521 cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc ttacgctaaa
     8581 gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga cgacgaagtt
     8641 cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc atccatcgaa
     8701 ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga cgcgatggaa
     8761 aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt acgtttcaac
     8821 tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca atctatgtaa
     8881 ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac gaaagtgggg
     8941 ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg actgccaaaa
     9001 cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc cacatctgga
     9061 gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt ggaaccgtag
     9121 acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc tgcctgtatt
     9181 gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt gaagatttaa
     9241 tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc gttaccgcat
     9301 ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc gcctgcaaaa
     9361 aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac gatccggtga
     9421 ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag atgaacgacg
     9481 agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc gccaaatatc
     9541 tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc tggtctgacg
     9601 atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac gttgagaaaa
     9661 tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg ggtgaagagt
     9721 acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg aaaggcattc
     9781 ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca actttattga
     9841 gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac atcttatagt
     9901 gcatctacca tatcccgtac ctgagataat aacgactgat aatattacgc cgatataaat
     9961 cgagttccgt cacctgtatt ttccgctcca ttgcccgctg atggatcgca tcagcaatat
    10021 gtgcggtcaa tgaatcatga cgaggatggg cccataccaa ataaatacgt tcagactgca
    10081 taattttacc tccacgttaa gttctggagc gcactttacg caggcggcat aataatgtaa
    10141 acgtgatgat tcaggatgag ttaatgaagg attttcacta atgcggatga atatgtctga
    10201 ctttgccact ttctttgctg tggcccgtaa tcaaagcttt cgtgcagcgg gcgatgagtt
    10261 aggcttatcc tcgtccgcca ttagccatag tattaaaaca ctggaacaac gtcttaaaat
    10321 tcgcttattc aatcggacaa cccgaagcgt atcgttaacg gaagcgggtt ctaatctcta
    10381 tgaacgattg cgcccagctt tcgacgaaat tcagatcatg ttagatgaga tgaatgattt
    10441 tcgtctgaca ccaacaggaa cgctcaaaat caatgccgcc cgggtagctg cacgtatatt
    10501 tttaatgcct ttactggttg gtttcacacg tgaatatcct gatattaaag tcgagctcac
    10561 aactgacgac tctcttgtcg atatcgtcca acagggcttt gacgctggcg ttcgacttag
    10621 tggcatcgtc gaaaaagata tgatcagcgt ggcgatcggc ccaccagtga aattatgtgt
    10681 agcagcaacg ccagagtact ttgcaagata tggcaaaccc cgccatcctc atgatctgct
    10741 taatcatcaa tgtgttgtat ttcgttatcc cagcggcaaa cctttccact ggcaatttgc
    10801 aaaggagcag gaaattgctg tcgcagggaa tatcattctg gat
//
LOCUS       DAJTVK010000136        10687 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000136 DAJTVK010000000
VERSION     DAJTVK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.136"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..784)
                     /locus_tag="OO372_003541"
     CDS             complement(<1..784)
                     /locus_tag="OO372_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342938.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="OO372_003542"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="OO372_003542"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6342939.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="OO372_003543"
     CDS             1860..2747
                     /locus_tag="OO372_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6342940.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="OO372_003544"
     CDS             2780..3790
                     /locus_tag="OO372_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCR6342941.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="OO372_003545"
     CDS             3860..5293
                     /locus_tag="OO372_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6342942.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="OO372_003546"
     CDS             5392..6741
                     /locus_tag="OO372_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6342943.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="OO372_003547"
     CDS             6731..7618
                     /locus_tag="OO372_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6342944.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="OO372_003548"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="OO372_003548"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6342945.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="OO372_003549"
     CDS             8352..9224
                     /locus_tag="OO372_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6342946.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="OO372_003550"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="OO372_003550"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6342947.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="OO372_003551"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="OO372_003551"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6342948.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2533 c         2854 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAJTVK010000137        10430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000137 DAJTVK010000000
VERSION     DAJTVK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.137"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="OO372_003552"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="OO372_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6342949.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="OO372_003553"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="OO372_003553"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6342950.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="OO372_003554"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="OO372_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCR6342951.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="OO372_003555"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="OO372_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6342952.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="OO372_003556"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="OO372_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="OO372_003557"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="OO372_003557"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6342953.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="OO372_003558"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="OO372_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6342954.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO372_003559"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="OO372_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6342955.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="OO372_003560"
     CDS             complement(9457..10149)
                     /locus_tag="OO372_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6342956.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2549 a         2516 c         2755 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAJTVK010000138        10363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000138 DAJTVK010000000
VERSION     DAJTVK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.138"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..154
                     /locus_tag="OO372_003561"
     CDS             <1..154
                     /locus_tag="OO372_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6342957.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="OO372_003562"
     CDS             258..1172
                     /locus_tag="OO372_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6342958.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="OO372_003563"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="OO372_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6342959.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="OO372_003564"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="OO372_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6342960.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..5066
                     /gene="ydeO"
                     /locus_tag="OO372_003565"
     CDS             4305..5066
                     /gene="ydeO"
                     /locus_tag="OO372_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6342961.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            5467..7149
                     /locus_tag="OO372_003566"
     CDS             5467..7149
                     /locus_tag="OO372_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6342962.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7201..8358
                     /locus_tag="OO372_003567"
     CDS             7201..8358
                     /locus_tag="OO372_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6342963.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8324..8446)
                     /locus_tag="OO372_003568"
     CDS             complement(8324..8446)
                     /locus_tag="OO372_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342964.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8655..10340
                     /locus_tag="OO372_003569"
     CDS             8655..10340
                     /locus_tag="OO372_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR6342965.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         3028 a         2208 c         2214 g         2913 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatgag tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacgctg
     5101 cgtaagaatt aaatggggca gccattccct gccccgcgtt gtttttaggc gatatattta
     5161 ttgaaataaa taagtgacat ccatcacata tttatgcact tgcataacct gttgcagatt
     5221 atttatgatc tcaattcggc attttgtcag taaaatgcaa taatttatta aatatcaata
     5281 aattagttgt ttatcggcga gaaattactt aatagaacag aaagtaatgt caacgcttta
     5341 tggactgtta tttccctttt tttagctaaa tctgctatct ctttatgtga ctaacttcac
     5401 ttacatccac ttatttctct tcgtaaaatt acttcggaat taagtacaat aagaagagga
     5461 acatttatga agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg
     5521 gcatctggta tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc
     5581 aaaacaaacg ttgctttctc agattttacg ccgactgaat acagtaccaa aggaaagcca
     5641 aatattatcg tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga
     5701 tcttttgacc caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata
     5761 gataaagcca ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa
     5821 ggcgtacgtt ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccgtgccgca
     5881 ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga tgctcaggat
     5941 ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg ttattacact
     6001 gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa
     6061 caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg gcaacctcaa
     6121 aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata ttacaactcc
     6181 ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta
     6241 accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt
     6301 tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga tcaatatcag
     6361 aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt ttattctgtt
     6421 gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta tgacaataca
     6481 attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc gctgaacggg
     6541 gcgcaaaaag gctataagag tcagacctat cctggcggta ctcacacccc aatgtttatg
     6601 tggtggaaag gaaaacttca acccggtaat tatgacaagc tgatttccgc aatggatttc
     6661 tacccaacag ctcttgatgc agccgatatc agcattccaa aagaccttaa gctggatggt
     6721 gtttccttgc tgccctggtt gcaagataag aaacaaggcg cgccacataa aaatctgacc
     6781 tggataacct cttattctca ctggtttgat gaggaaaata ttccattctg ggataattac
     6841 cacaaatttg ttcgccatca gtcagacgat tacccgcata accccaacac tgaggactta
     6901 agccaattct cttatacggt gagaaataac gattattcgc ttgtctatac agtagaaaac
     6961 aatcagttag gtctctacaa actgacggat ctacagcaaa aagataacct tgccgccgcc
     7021 aatccgcagg tcgttaaaga gatgcaaggc gtggtaagag agtttatcga cagcagccag
     7081 ccaccgctta gcgaggtaaa tcaggagaag tttaacaata tcaagaaagc actaagcgaa
     7141 gcgaaataac taaaccttca tgcggcggat ttttccgccg ccttattgag cgagatagcg
     7201 atgcacgtta cagccaagcc ctccagtttt caatgtaatc tcaaatgtga ttactgtttt
     7261 taccttgaaa aagagtcgca gtttactcat gaaaaatgga tggatgacag cactctgaaa
     7321 gagttcatca aacaatatat cgaagcgtct ggcaatcagg tctattttac ctggcaaggc
     7381 ggtgaaccca ctctggctgg cctggatttt ttccgtaaag ttattcacta tcaacaacgc
     7441 tatgcaggcc aaaaacgtat ttttaatgca ttacaaacga atggcatttt attgaataat
     7501 gaatggtgtg cctttctcaa agaacatgaa tttctggttg gtatctcgat cgatggcccc
     7561 ctggagttac atgaccgtta cagacgcagt aattcaggta acggtacttt tgcaaaagtg
     7621 atagcagcca tcgagcgtct gaaatcatat caagtagagt ttaatacgtt aaccgtcatt
     7681 aataacgtta atgtccatta ccctcttgag gtttatcatt ttttaaaatc tatcggcagt
     7741 aaacatatgc aatttatcga attgctagaa accgggacgc cgaatattga tttcagtggt
     7801 catagtgaga acacattccg tatcattgat ttttctgtgc ctcccacggc ttatggcaag
     7861 tttatgtcaa ccatttttat gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt
     7921 cagtttgaaa gctttgtcag ccgttttttg gggaatgggc atacaagttg tattttccag
     7981 gagtcctgca aggataatct ggttgttgaa agtaatggag acatttacga atgcgaccat
     8041 tttgtctatc cacagtacaa aattggaaac attaataaat ctgaactcaa aacgatgaac
     8101 agtgtacaac tgacagcgca aaaaaaacgg atttcagcga aatgtcagca atgtgcatat
     8161 aaacctatct gcaatggcgg ttgtcctaag catcgtatta ctaaagtaaa caatgagact
     8221 gtttcttatt tttgcgaagg ttataaaatc cttttttcaa ccatggtacc ttatatgaac
     8281 gccatggttg agttagctaa gaacagagta ccgctttacc acattatgga tgttgcaaaa
     8341 caaatggaga ataattaata acgctctctt ttaactccat tcttagcctc cttcttatga
     8401 ccttaacaag gcacccgaca taaattcctg tcgggtgttt cgtcatcatc atcttccctt
     8461 gcgaaaatcc tggcttacgt ggtatgcgtc agggcattag caataccctt gattatcaat
     8521 agcataacca taaccataac cactgaaaat taaaaatttc tatatatttc atattgttaa
     8581 atgatactta aataaatttc atcagtaaat cattgccata gataatcatt ggatggcaca
     8641 atcaattttc gctaatgata accattccca ttacgtttta tatgttgata gcaaagtatc
     8701 tctgtctgct gaagcccttt tggctgcgta agaataataa aacttcagtg ttgttaatca
     8761 ttattatcct cgcaatgatc cttggcgtgg ttaaaattca ggtctggtta aacgactgga
     8821 ataatgattt tttcaatgcg ctaagccaga aagaaaccga taagctttgg cagcttgttc
     8881 tttggttccc tgccctgctg gggatctttg tattaatatc cgtaaacaaa acatggttaa
     8941 ttaaactact gactattcgc tggcgcgaat ggttaacaga ttactacctt aatcgctggt
     9001 ttgcagataa aaactactac ttcacgcaga tctacggcga gcataaaaat acagataacc
     9061 ctgaccaacg tatcgctgaa gacattctct tactgatcag caaaacgctc agtctttcat
     9121 ttggcttcat ccagtcactt agcatgctga tcacctttac cgttattctt tggcaaagcg
     9181 cgggtacgct ctcattcact gttggtggaa cagaatggaa tatccagggg tatatggtct
     9241 ataccgtcgt gctcattgtg atcggtggaa ctttatttac ccataaggtg ggtaaacgta
     9301 ttcgtccgct taatgtggaa aaacaacgta gcgaagcgac atttcgaact aatcttgtgc
     9361 agcataataa gcagacagaa ctgattgcgc taagcaatgc tgagtctcta caacgccagg
     9421 agttgcgcga caattttcat accattaaag agaactggca tcgtttaatg aatcgtcagc
     9481 ggtggcttga ttactggcaa aatatttatt cgcgctcgct tagcgttctt ccctactttc
     9541 tgttattgcc gcagtttatt agcgggcaga tcaatctggg cggactgatg aaatcgcgcc
     9601 aggcatttat gctagtatcg aacaatttaa gctggtttat ttataaatat gacgaacttg
     9661 ctgaactggc tgcggttatc gatcgcttgt atgagttcca tcaactcact gaacagcgcc
     9721 ctacgaataa gcctaaaaat tgccaacatg cggtacaagt ggctaatgcg agtattcgta
     9781 cgcctgataa taagatcata ttagagaact tgaactttca tgtttcgcca ggcaaatggc
     9841 tattactgaa aggctactct ggtgcgggaa aaaccacact gcttaaaaca ttatcccact
     9901 gctggccgtg gtttaaaggt gatatttctt ctcctgctga cagttggtat gtgtcacaaa
     9961 caccgttaat caaaagcggc ttactgaaag agattatttg taaagcactt cccctgcccg
    10021 tagacgataa atcgttgagc gaagtactgc atcaggttgg tctggggaaa ttggctgcgc
    10081 gtattcatga ccacgatcgc tggggagata ttctttccag cggcgaaaaa caacgtatcg
    10141 ccctggcacg attaatttta cgacgtccga aatggatatt tcttgacgaa actacctctc
    10201 atcttgagga acaagaggct atacgcttac tgcgtttagt gcgtgaaaaa ctacccacaa
    10261 gcggcgtcat tatggttaca catcaacccg gtgtctggaa cctggctgat gatatttgtg
    10321 acattagcac ggttttataa caacaataaa agccctgctg tac
//
LOCUS       DAJTVK010000139        10340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000139 DAJTVK010000000
VERSION     DAJTVK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.139"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(41..1045)
                     /gene="cra"
                     /locus_tag="OO372_003570"
     CDS             complement(41..1045)
                     /gene="cra"
                     /locus_tag="OO372_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6342966.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1127..1213)
                     /locus_tag="OO372_003571"
     CDS             complement(1127..1213)
                     /locus_tag="OO372_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6342967.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="OO372_003572"
     CDS             complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="OO372_003572"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6342968.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="OO372_003573"
     CDS             complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="OO372_003573"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6342969.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3562..3606
                     /locus_tag="OO372_003574"
     CDS             3562..3606
                     /locus_tag="OO372_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6342970.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="OO372_003575"
     CDS             complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="OO372_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6342971.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="OO372_003576"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="OO372_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6342972.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="OO372_003577"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="OO372_003577"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6342973.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="OO372_003578"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="OO372_003578"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6342974.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="OO372_003579"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="OO372_003579"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6342975.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..10225
                     /gene="leuD"
                     /locus_tag="OO372_003580"
     CDS             9620..10225
                     /gene="leuD"
                     /locus_tag="OO372_003580"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6342976.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2711 a         2717 c         2523 g         2389 t
ORIGIN      
        1 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
       61 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
      121 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
      181 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
      241 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
      301 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
      361 ataactgggc agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
      421 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
      481 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
      541 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc
      601 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
      661 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac
      721 agcgcatttc gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt
      781 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
      841 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
      901 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
      961 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
     1021 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
     1081 attgtactgg tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac
     1141 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
     1201 gcaaattgcg cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
     1261 cacaccagaa cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc
     1321 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
     1381 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
     1441 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
     1501 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
     1561 gcccacggtc tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag
     1621 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
     1681 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
     1741 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca
     1801 tcgacggtaa catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta
     1861 agtttgcttt ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc
     1921 gccagacgga cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag
     1981 atcatgtcct gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt
     2041 acgggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg
     2101 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
     2161 ggtaaaccaa aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg
     2221 aatggataat aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
     2281 tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
     2341 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg
     2401 cggatctcat ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
     2461 aggacctggc gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa
     2521 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc
     2581 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
     2641 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
     2701 ggaaacgctc ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt
     2761 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
     2821 ttttttgccg ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
     2881 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
     2941 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc
     3001 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
     3061 tgtttaacca ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
     3121 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
     3181 gtggcgatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
     3241 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
     3301 actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
     3361 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
     3421 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
     3481 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
     3541 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
     3601 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
     3661 atcctaaaat agagagccca cacattcaga ataaaattct acattcaata aaaaatacag
     3721 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
     3781 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
     3841 gataacaggt tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
     3901 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
     3961 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
     4021 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
     4081 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
     4141 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
     4201 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
     4261 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
     4321 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
     4381 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
     4441 ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
     4501 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
     4561 acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
     4621 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
     4681 aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
     4741 ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
     4801 aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat
     4861 ccattgccgt ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga
     4921 aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc
     4981 ttatttaata attaatttat atatttaatg cattaattct taacattaat tgatcaataa
     5041 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
     5101 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
    10261 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
    10321 taaccgcaat tacccagtag
//
LOCUS       DAJTVK010000140        10211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000140 DAJTVK010000000
VERSION     DAJTVK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.140"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(127..909)
                     /locus_tag="OO372_003581"
     CDS             complement(127..909)
                     /locus_tag="OO372_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6342977.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(906..1979)
                     /locus_tag="OO372_003582"
     CDS             complement(906..1979)
                     /locus_tag="OO372_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6342978.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(2101..2262)
                     /locus_tag="OO372_003583"
     CDS             complement(2101..2262)
                     /locus_tag="OO372_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6342979.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(2389..2994)
                     /gene="osmY"
                     /locus_tag="OO372_003584"
     CDS             complement(2389..2994)
                     /gene="osmY"
                     /locus_tag="OO372_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6342980.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(3387..4976)
                     /gene="prfC"
                     /locus_tag="OO372_003585"
     CDS             complement(3387..4976)
                     /gene="prfC"
                     /locus_tag="OO372_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6342981.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(5067..5744)
                     /gene="yjjG"
                     /locus_tag="OO372_003586"
     CDS             complement(5067..5744)
                     /gene="yjjG"
                     /locus_tag="OO372_003586"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6342982.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(5759..6205)
                     /gene="rimI"
                     /locus_tag="OO372_003587"
     CDS             complement(5759..6205)
                     /gene="rimI"
                     /locus_tag="OO372_003587"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6342983.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(6174..6587)
                     /gene="holD"
                     /locus_tag="OO372_003588"
     CDS             complement(6174..6587)
                     /gene="holD"
                     /locus_tag="OO372_003588"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6342984.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            6690..7721
                     /gene="rsmC"
                     /locus_tag="OO372_003589"
     CDS             6690..7721
                     /gene="rsmC"
                     /locus_tag="OO372_003589"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6342985.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            7989..8075
                     /locus_tag="OO372_003590"
     tRNA            7989..8075
                     /locus_tag="OO372_003590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8023..8025,aa:Leu,seq:cag)
     gene            8110..8196
                     /locus_tag="OO372_003591"
     tRNA            8110..8196
                     /locus_tag="OO372_003591"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8144..8146,aa:Leu,seq:cag)
     gene            complement(8235..8471)
                     /locus_tag="OO372_003592"
     CDS             complement(8235..8471)
                     /locus_tag="OO372_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6342986.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            8612..9400
                     /gene="fhuF"
                     /locus_tag="OO372_003593"
     CDS             8612..9400
                     /gene="fhuF"
                     /locus_tag="OO372_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6342987.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(9438..10115)
                     /gene="bglJ"
                     /locus_tag="OO372_003594"
     CDS             complement(9438..10115)
                     /gene="bglJ"
                     /locus_tag="OO372_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6342988.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(10073..>10211)
                     /locus_tag="OO372_003595"
     CDS             complement(10073..>10211)
                     /locus_tag="OO372_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR6342989.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT         2426 a         2749 c         2566 g         2470 t
ORIGIN      
        1 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
       61 aagacgcgtt aagcgtcgca tcaggcatta tgcgccgact gccggatgct gcctacgaag
      121 ggtaggctac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
      181 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
      241 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
      301 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
      361 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
      421 caggctgccg gaaaaaccgt gaaccacgcc agtgcacggt aaatcgtgac gtttaagatg
      481 catcgccagt ttgtcgtgta tacgccggga atgaaggatc accggcagat cgtagcgttt
      541 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaaattgcg gatcgtcgcc
      601 gaagagatcc agaccgatct cccccaccgc caccaccttc gccggacgcc tttccagcgc
      661 ctgctgtagc tgatcaagag acacatcgct atgtttttcc aacatccccg gatgcaaccc
      721 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacgcggg caaaattttc
      781 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
      841 ggcctcttca tcgccactaa acggcgggaa atcaaaatgg cagtgggtat cgataaaacg
      901 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcatcgctc
      961 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     1021 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     1081 gtggcgagga aataacgacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     1141 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     1201 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     1261 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     1321 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     1381 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     1441 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     1501 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     1561 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     1621 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     1681 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
     1741 ttgcgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     1801 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     1861 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     1921 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     1981 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     2041 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     2101 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     2161 gacaatttta gctgcgcctg cagcggtacc ggccagacca ccaaacccaa gtgcggcggc
     2221 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     2281 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     2341 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     2401 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     2461 gctctgtgcc tgggaatcga cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     2521 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     2581 ggtggcggtg tcacccgcgt agcccttcac agagccttct ttagcgtcgc gaacgtgcag
     2641 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     2701 ctgagcctgg ctttcaacga aaccgctcag ggtcacgact ttttgatcgg ttttgacaga
     2761 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     2821 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     2881 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     2941 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     3001 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     3061 gagagacttc accctctaca gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa
     3121 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
     3181 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     3241 gttaaatata gatcacaatt ttgaaaccgc tcgggatatc agcgaaaaca taagcaaaag
     3301 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     3361 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     3421 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     3481 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     3541 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     3601 acgctgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     3661 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     3721 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     3781 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     3841 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     3901 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     3961 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     4021 cgcataaacg ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat tttaaatacg
     4081 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     4141 ggcgcaggag cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     4201 gcagtaccga agaataccgg agtgatttcg cccgcaagga acaattcttt gtcgaactcg
     4261 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     4321 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     4381 tggcctttac cgctctgata gagataggtt tcgtctttat aaaggtggta aacgccttta
     4441 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc
     4501 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     4561 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     4621 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgccgtcag ggtacgatag
     4681 gtatcttccg agaagtcttc gtgccccggg gtgtcgagca ggttaaccag gcaatcgtga
     4741 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     4801 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccggc ggtctgaatg
     4861 gcctgtccga acagcagcac cttctcggtg atggtagtct taccggcatc cgggtgagaa
     4921 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     4981 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     5041 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
     5101 aacgaagaaa cggtccaggt gggcgcgatg ccttctggtt gctcgcgatt gtgcgcattc
     5161 agccagcagg tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
     5221 ccaaccatca gcacgcgtga acggtcagga ttgcccgcct gttccagcgc ataatcgaaa
     5281 attttcttat tcggtttggc aacgccaact tcttcggaaa tcaccagcaa atcgaagtaa
     5341 tcacgcaggc ccgtgcgttc cagacgcact tgctgcaagg cgctaaagcc gttggtgatg
     5401 atgccgattt tggcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     5461 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     5521 agccgttcgg cccagctctc aaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     5581 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     5641 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     5701 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     5761 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     5821 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     5881 catttgaggc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     5941 tcagatgttc cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccgcaa
     6001 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
     6061 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aaggtttttt
     6121 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     6181 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     6241 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     6301 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     6361 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     6421 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     6481 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     6541 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     6601 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     6661 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     6721 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     6781 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     6841 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     6901 tggatgacgt cgcagattgc gatatgctga tttactactg gccgaagaac aaaccggaag
     6961 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     7021 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     7081 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     7141 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
     7201 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     7261 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     7321 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     7381 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     7441 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     7501 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc
     7561 gtcatcttaa tagcggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttacccgg
     7621 acgtgctgga tgagacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     7681 tgtatcgcgc cattatgacc cgccaggcga agaaaggtta attatctcgc cgaataccgt
     7741 gtcggatgcg gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
     7801 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
     7861 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
     7921 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
     7981 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgttctta
     8041 cggacgtggg ggttcaagtc tcccccctcg caccaaacga ggcgatatca aaaaaagtaa
     8101 gatgactgtg cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgtcctt
     8161 acggacgtgg gggttcaagt cccccccctc gcaccaatta tctttacttc ctttcttgtt
     8221 tcttccttga tttttcatcc ctgattcaaa ttcattatca tcagcatgat ggcagcaata
     8281 agcgccaggc acgatggtag caaaacgtaa tgccgtaact gtttgtgata cagcatcacc
     8341 ggcgtcaata ctaatcccat cagaatgaca atccgccact gatccggcga taaatccgca
     8401 tacatcagcc ctgcaatcag caatcccggc agcaacactc cccagccact gcccagcgta
     8461 cgttgcaaca tggtttcatc tctttcattg ataatgataa ccaatatcat atgataattt
     8521 ttatcatttg caagccagag aaatcccttg ctatcgggta aacctatcgc taggattagc
     8581 aatcattatc atttagatta ctatcccgat tatggcctat cgttccgcac cgctctatga
     8641 agatatcatc tggcgaacgc atctccagcc gcaggatgct gggcttgcac aagccgtacg
     8701 tgcgacgatc gccaaacatc gtgaacattt gctcgagttt atccgcctgg atgaacccgc
     8761 tccgcttaac gccatgacgc tggcgcaatg gtcatcaccg aatgcgctaa gttctctact
     8821 cgcggtctat tccgatcata tctatcgcaa ccaaccaacg atgatccgcg agaacaaacc
     8881 gctgatctca ctatgggcac aattgtatat cggcctgatg gtgccaccat taatgctggc
     8941 gctactgacg caggaaaagg cattagatgt gtcgccggaa catttccatg ctgagtttca
     9001 cgaaaccgga cgcgtcgcct gtttctgggt cgatgtgtgt gaagataaaa acgcaacacc
     9061 acattcgccg cagcagcgaa tggaaacgtt aatcagccag gcgctggttc cggttgtgca
     9121 ggcactagaa gcgaccggag agatcaacgg caaacttatc tggagtaata ccggttattt
     9181 gatcaactgg tatctcactg agatgaagca actgctcggc gaggcgacgg ttgaatcgct
     9241 gcgccatgcc ctcttttttg agaaaacgct cacgaacggt gaagataatc cactctggcg
     9301 taccgtcgtg ctgcgcgatg gcctgctggt gcgccgaacc tgctgccagc gttatcgctt
     9361 accggatgtg cagcaatgtg gcgattgtac gctgaaatga aagcactgcc ggggaagtaa
     9421 acccggcatc atgcggatta atagggatgc aacacattac ttgcttcgca atgccgcata
     9481 cataacagaa tatctgcggc ctccaacaac cctgcatcag aactgacgcc cagtttcgac
     9541 atcacattaa atttatgtgc acggatcgtt ttgatattgc gtttaagctg ctcggcaatt
     9601 tgtgtcattg agtagccacg cgacataaag cgcaatattt ctctctccgt cgggcttagc
     9661 gtccggcttt ggttaatgta ccactgattg ttcagtcggt cggtcgcctg acgtacacca
     9721 tttaatgaca aaaagagttc ctgatgaaaa atctccagcg ttgacgcttt acttaataca
     9781 ccgtccagcg gtgatggcga caatgaacca atcagccgag cttcaatatc atcatccgca
     9841 ataactaaac gccgggtacg cggaaactta atcgccagtt cagtcaggca agataatccc
     9901 tctctgcgct cacttctcat ggcagaaaaa gaaaaaatga ccgccgcaaa tgagatccgc
     9961 gacatggcat cctgaaatga ctcctgactg gtataggtat ggagcttata agggttaccc
    10021 gcaaactttc tgaataaact ctcaataccg atactgctca tgacgcattt ttctatgagt
    10081 gcgacatttc tcttcttaat tcggctgtgt tccattctgc ggcatctcca taaacagaag
    10141 gcaagttaag ttctcttgca ccctgcgcgc tatttatcca tgcatacatt tcagcgttgc
    10201 tgtgaattgc c
//
LOCUS       DAJTVK010000141         9852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000141 DAJTVK010000000
VERSION     DAJTVK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.141"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OO372_003596"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="OO372_003596"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6342990.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="OO372_003597"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="OO372_003597"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6342991.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="OO372_003598"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="OO372_003598"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6342992.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="OO372_003599"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="OO372_003599"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6342993.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="OO372_003600"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="OO372_003600"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6342994.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="OO372_003601"
     CDS             5058..6191
                     /locus_tag="OO372_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6342995.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="OO372_003602"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="OO372_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6342996.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OO372_003603"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="OO372_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6342997.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OO372_003604"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="OO372_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6342998.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OO372_003605"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="OO372_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6342999.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OO372_003606"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="OO372_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6343000.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="OO372_003607"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="OO372_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6343001.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9852
                     /locus_tag="OO372_003608"
     CDS             9762..>9852
                     /locus_tag="OO372_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343002.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2518 a         2504 c         2502 g         2328 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaatg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg ca
//
LOCUS       DAJTVK010000142         9821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000142 DAJTVK010000000
VERSION     DAJTVK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.142"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(39..1034)
                     /gene="gapA"
                     /locus_tag="OO372_003609"
     CDS             complement(39..1034)
                     /gene="gapA"
                     /locus_tag="OO372_003609"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6343003.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1376..1789
                     /gene="msrB"
                     /locus_tag="OO372_003610"
     CDS             1376..1789
                     /gene="msrB"
                     /locus_tag="OO372_003610"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6343004.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1831..2103
                     /locus_tag="OO372_003611"
     CDS             1831..2103
                     /locus_tag="OO372_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6343005.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="OO372_003612"
     CDS             complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="OO372_003612"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6343006.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="OO372_003613"
     CDS             complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="OO372_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6343007.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASARGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="OO372_003614"
     CDS             complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="OO372_003614"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6343008.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6116..6667
                     /locus_tag="OO372_003615"
     CDS             6116..6667
                     /locus_tag="OO372_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6343009.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6784..7827
                     /gene="selD"
                     /locus_tag="OO372_003616"
     CDS             6784..7827
                     /gene="selD"
                     /locus_tag="OO372_003616"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6343010.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7832..9793
                     /gene="topB"
                     /locus_tag="OO372_003617"
     CDS             7832..9793
                     /gene="topB"
                     /locus_tag="OO372_003617"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6343011.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2434 a         2452 c         2618 g         2317 t
ORIGIN      
        1 ccgaagtcgc tctttttaga tcacagtttc atctcaactt atttggagat gtgagcgatc
       61 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
      121 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
      181 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
      241 tcagcagcgg ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
      301 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
      361 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
      421 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
      481 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
      541 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
      601 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
      661 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
      721 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc
      781 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
      841 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
      901 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
      961 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     1021 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     1081 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     1141 tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg
     1201 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     1261 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     1321 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc
     1381 taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     1441 gcagaatcat gggacagagc cgccatttac gggtcgttta ctgcataaca agcgtgacgg
     1501 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tcccaaacca agtatgattc
     1561 cggctgtggt tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     1621 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     1681 ggggcatgtc ttccctgacg ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc
     1741 ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     1801 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     1861 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcccgatggc
     1921 gttgcgttga ccgaggaaca aaaagcgaac tgcctgcaac tggtgatgct gtggcaagct
     1981 cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     2041 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag
     2101 tgaatgagtt atctagtatt taagcctgtt attaagggag ggcaaatata cttgttagga
     2161 cgtcgtgttt tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca
     2221 atgtcattga ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt
     2281 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgcg
     2341 cactgtcctg gggctggata ttcacgccac gacaaccatc ggtaatcaca tttaccttat
     2401 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
     2461 taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagcgag gttttctgcc
     2521 gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
     2581 acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct
     2641 gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag
     2701 gctctacacc gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag tcctgactgg
     2761 cgataaccgc ttcaccgcgc gactggcacc agtcaatcag gcggttagcg acatccaccg
     2821 tactgtcacc ttccggcacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
     2881 ccagtaacag ggcgcgaggg ggcattacag tctccttaat catccggcgt cagttcgccg
     2941 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
     3001 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca
     3061 tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt
     3121 gtcaggttga ccaccacaat accgcgagcg ctggcttctt gtaattcctg caggaaggct
     3181 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg
     3241 cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac tacgccaatt
     3301 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
     3361 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
     3421 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
     3481 aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga
     3541 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
     3601 ggtttaccga gattctcgag catgaacgac agcgcagagg cagtatacgc catcgtgtcg
     3661 gtgccgtgca ggatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
     3721 atatgctgcc agtcttctgg cgtcatatca gaagaatcca tcagcggcgt atattcatga
     3781 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
     3841 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
     3901 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
     3961 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
     4021 gggcgagaca gcgggaagag gattcgtcga cgtaaaaaat aatgtggccg acgatctgtc
     4081 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
     4141 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
     4201 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
     4261 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
     4321 ccactgtttc acttttgcca gctctgctgc tttggcgacc gcatcatcga aatccccgag
     4381 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
     4441 tttatcaatc tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
     4501 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
     4561 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
     4621 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
     4681 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
     4741 gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg
     4801 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
     4861 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
     4921 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
     4981 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
     5041 atcagtctta ctccagccga actctttggt tagagctttt tcgatttcgg cactcgatgc
     5101 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
     5161 taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt
     5221 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
     5281 gcgggctgcc ggtgacatgt catcacgaat aaacggttca acggcagatt tatacgtacc
     5341 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
     5401 gttggtagca aaaccgtgca aatccaccac gccttgcggt gacagccaaa ttttattggc
     5461 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
     5521 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
     5581 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg
     5641 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
     5701 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac
     5761 accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
     5821 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
     5881 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
     5941 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
     6001 gtggttatcg taatgacaca accggcactt gtcagcagga attgcggact gtgctgtaac
     6061 aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga
     6121 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
     6181 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
     6241 gcaaccgtgg catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccgtact
     6301 ggaacagggg gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc
     6361 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt
     6421 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
     6481 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
     6541 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
     6601 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
     6661 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
     6721 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
     6781 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
     6841 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
     6901 ccgaatttgc ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat
     6961 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
     7021 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
     7081 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
     7141 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
     7201 cactccatcg atgcgacgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
     7261 gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg
     7321 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag
     7381 ggactagcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
     7441 gaaggcgtaa aagcgatgac cgacgttacg ggttttggtc tgctgggcca tttgagcgaa
     7501 atgtgtcagg gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc aaaattgccg
     7561 ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc
     7621 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg
     7681 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct
     7741 acagccgccg agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc
     7801 ggtcgtgcca tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag
     7861 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
     7921 cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca
     7981 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattatccc
     8041 ggaaaagtgg caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg
     8101 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact
     8161 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
     8221 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgctcg accgtcttcg
     8281 gtccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
     8341 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
     8401 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
     8461 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
     8521 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
     8581 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
     8641 tggtcaacca gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
     8701 gcctttttcg ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca
     8761 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
     8821 ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc
     8881 catcagcgtt catgcaccag atctgttgct gcagccagtg gtagatccag atatacgcaa
     8941 ccgctgttgg gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag
     9001 ttcagccatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta
     9061 cttgatgcag ttctgcccgg atgtggtgtt ccgcaagtgt gttatcgaac tggacattgc
     9121 caaaggcaaa tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt
     9181 aggcagcaaa gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg
     9241 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
     9301 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
     9361 agatctgaaa aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
     9421 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
     9481 taccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
     9541 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
     9601 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
     9661 ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa
     9721 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg
     9781 ggcgatagcg taagcgagtg aatctttcgt gctattcgag t
//
LOCUS       DAJTVK010000143         9645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000143 DAJTVK010000000
VERSION     DAJTVK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.143"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..667)
                     /locus_tag="OO372_003618"
     CDS             complement(<1..667)
                     /locus_tag="OO372_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6343012.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO372_003619"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="OO372_003619"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6343013.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="OO372_003620"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="OO372_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6343014.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="OO372_003621"
     CDS             complement(5698..7014)
                     /locus_tag="OO372_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR6343015.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="OO372_003622"
     CDS             complement(7004..8761)
                     /locus_tag="OO372_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6343016.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9640)
                     /locus_tag="OO372_003623"
                     /pseudo
     CDS             complement(8777..9640)
                     /locus_tag="OO372_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2449 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcga
//
LOCUS       DAJTVK010000144         9574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000144 DAJTVK010000000
VERSION     DAJTVK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.145"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..158)
                     /locus_tag="OO372_003624"
     CDS             complement(<1..158)
                     /locus_tag="OO372_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6343017.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPA"
     gene            complement(158..1084)
                     /gene="glsB"
                     /locus_tag="OO372_003625"
     CDS             complement(158..1084)
                     /gene="glsB"
                     /locus_tag="OO372_003625"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6343018.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1148..2536)
                     /gene="sad"
                     /locus_tag="OO372_003626"
     CDS             complement(1148..2536)
                     /gene="sad"
                     /locus_tag="OO372_003626"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR6343019.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2637..3518
                     /locus_tag="OO372_003627"
     CDS             2637..3518
                     /locus_tag="OO372_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6343020.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3595..4710
                     /gene="yneK"
                     /locus_tag="OO372_003628"
     CDS             3595..4710
                     /gene="yneK"
                     /locus_tag="OO372_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6343021.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4860..6050
                     /gene="ydeA"
                     /locus_tag="OO372_003629"
     CDS             4860..6050
                     /gene="ydeA"
                     /locus_tag="OO372_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6343022.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6075..6740)
                     /gene="marC"
                     /locus_tag="OO372_003630"
     CDS             complement(6075..6740)
                     /gene="marC"
                     /locus_tag="OO372_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6343023.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6952..7386
                     /gene="marR"
                     /locus_tag="OO372_003631"
     CDS             6952..7386
                     /gene="marR"
                     /locus_tag="OO372_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6343024.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7406..7789
                     /gene="marA"
                     /locus_tag="OO372_003632"
     CDS             7406..7789
                     /gene="marA"
                     /locus_tag="OO372_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6343025.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7821..8039
                     /gene="marB"
                     /locus_tag="OO372_003633"
     CDS             7821..8039
                     /gene="marB"
                     /locus_tag="OO372_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6343026.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8096..9535)
                     /locus_tag="OO372_003634"
     CDS             complement(8096..9535)
                     /locus_tag="OO372_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6343027.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2466 a         2458 c         2162 g         2488 t
ORIGIN      
        1 gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct
       61 ttctccaggc aatactgacg ctctttcatg cccagacgaa agcgagagcg aaattttgaa
      121 cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa cgccctaact
      181 gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt
      241 ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa
      301 cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac
      361 cactggtcgc catcagcgcg ttaatttgcc gcgcctgcat tggcgtcact acaggttcat
      421 caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgagcc agctctacac
      481 agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc acgtcatgat
      541 ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa
      601 attcggaacg cgctaccacc gtatcgtatg aaatatcaga cacaccgctt aagccgcgca
      661 cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca
      721 ccagcgcacc agcattaata aacggattac gcggaatacc ctgttccatt tccagttgca
      781 ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt
      841 cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa
      901 aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc
      961 ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac
     1021 caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg
     1081 ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata
     1141 tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga
     1201 aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt
     1261 cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc
     1321 tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag
     1381 ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg gggccaaaca
     1441 tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt
     1501 agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg
     1561 ttttctctac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt ggtccgagag
     1621 tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt
     1681 cggtaaatgc agaagcaatt ccctcttcga taataaagcg ttttgccgct gcacatacct
     1741 gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc agatcggcat
     1801 cgttaagcac aataaacgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg
     1861 ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcacc gtgacagcag
     1921 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     1981 atacgccttg cgggattccc gcatctttaa acacctgggc aatgagctga gcacatccca
     2041 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     2101 cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg
     2161 gtcgatactc aataaccgcc tgctgatttt ccaccagtgt aggttccgcc ttcagcattg
     2221 ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg
     2281 cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg
     2341 cgcgcagagc cttaccgata tcacgcattt tttcagcacg ataatctata tttgtctcgc
     2401 gccagtcgcg aaagcctgct gccgccagct caagtgcgtt ttcgatatcg ttagcgccag
     2461 cccacggcag cacagaaagt tgttcacccg tggcaggatt tattgaaatt gcatgagttg
     2521 ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt
     2581 tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag aatgttatgg
     2641 atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acccaggctg
     2701 ctgcaaaagt gcatcgcgta ccgtcgaatc tcactacccg tctacgccag ctggaaacag
     2761 aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctctct cctgccgggc
     2821 ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg
     2881 tcgctggcga tgagccgcaa ggtttatttt ctcttggttc gctggaaagc accgcagcag
     2941 tgcgcattcc ggccacgctg gcggaattca accatcgtta tcccaaaatt cagttttcac
     3001 tttccaccgg cccttccggc actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg
     3061 catttattga tgggcccatt aaccatactg ccattgacgg gataccggta taccgcgagg
     3121 agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgcgccagt caggttaacg
     3181 gcagtaacat ttatgccttc cgcaccaatt gttcgtatcg tcgccacttc gagagctggt
     3241 ttcatgctga cggtgccgct ccgggaacta ttcacgagat ggagtcgtat cacgggatgt
     3301 tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc acgctctatg ctggaaagta
     3361 tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt tggttaacaa
     3421 cctggctggt ctggcgtcgc ggtgcgaaaa cgcgtccgct tgaggcattt attcaactac
     3481 tggatgtgtc tgactcggca aaacagagtt accaatgaac tattttgata attcttgcgg
     3541 ctaatatgct ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt
     3601 acgccagtaa gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt
     3661 gcaccacagg taaaagaagt tttaaaggac tttattgatg cgctaagtac aataatatgt
     3721 gatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac
     3781 cttgataact tatcatatat tcagaggact tctttccggg gtaacgacac gctatacaat
     3841 gaaaaggttc agtttaaact cacttatccc gtcaagaatg gaagacacaa agaaaatatt
     3901 gaatttcagg tagtaataaa tttaagccct atttatttag ataattttcg ccatgatgga
     3961 gaaattaata ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag
     4021 accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg
     4081 ttcaaccctg gcgttccgat caaaatagcg catacaccac atattgaacc cctgcctccg
     4141 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
     4201 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
     4261 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
     4321 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc
     4381 gaagtctgca tctgcttttt attcctttta gcagaacaag aatctcctca ttttcaggac
     4441 ttgtttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa
     4501 agtgatttga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaagcat
     4561 atggcgcgca ggaccctcga actgtttgta aaaaatgaaa caccgccaaa agttactcac
     4621 gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc
     4681 aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga
     4741 acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaataca ttgtattcac
     4801 atcattaacc gttttaagat catttcatca ctttttcgca actcacccga taatctgtta
     4861 tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg
     4921 ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc
     4981 aaagttttca catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag
     5041 tagcgctaat gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac
     5101 tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct
     5161 ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga
     5221 ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt
     5281 taattgccac cggtacagca ctggcgatgg tcttaggttt acctctcggg cgcattgtgg
     5341 ggcagtattt cggttggcga atgaccttct tcgcgattgg tattggggcg cttatcaccc
     5401 ttttgtgcct gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa
     5461 gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg
     5521 ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg
     5581 gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg
     5641 gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg agtacggcga
     5701 ttgcgctgtt gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc
     5761 tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg
     5821 ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca
     5881 tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt
     5941 caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa
     6001 tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc
     6061 cattcgggcc ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac
     6121 tgcacaccca tacataccag caggaatccc atcagacggg agatcgcttc aatgccaccc
     6181 ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc
     6241 accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc
     6301 tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc agcggtactt
     6361 ggcattgcta gcggcacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac
     6421 ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaagagcat ccgaaaaccg
     6481 ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta
     6541 tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac gtacaccgag
     6601 gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat
     6661 agcgcgacag ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt
     6721 gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa
     6781 gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc
     6841 aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact
     6901 tgcctgggca atattatcct ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt
     6961 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     7021 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     7081 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     7141 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     7201 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     7261 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     7321 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     7381 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     7441 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     7501 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     7561 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     7621 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     7681 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     7741 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aatagctagt tgataacgtg
     7801 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     7861 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     7921 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
     7981 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
     8041 tattgtcagg ctctgatcac agagcctgac atctccgtaa tatcttcctc aagtctcaaa
     8101 tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc
     8161 gttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgctttt
     8221 tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggggtg tagccaatta
     8281 agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat
     8341 caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc
     8401 cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac
     8461 gtagccctgc cggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga
     8521 ccaaatctcg tggttcaaca taatccagtt gcgggttatc gggagaaaat tgtgtcgtaa
     8581 aagacatgta gtagctaaaa ccgataaagt cgacacatcc actggcaaga atcgcgttat
     8641 cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg
     8701 gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtgg
     8761 ccatcatcat atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatac
     8821 agccaatgtt aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa
     8881 ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctctg
     8941 gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat
     9001 tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg
     9061 ttgatgcgaa gcggataaaa aagtcgatca gcttacggtt tcgccagcca ccatattttg
     9121 tcaccagatg ataaggcatc tcaaaatgcg aaagcgtcac cacaggttcc attccttgct
     9181 tcaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc ggctcctgtt
     9241 cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat ttgaatccca
     9301 tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt gcttcatgat
     9361 tgggataatt aagcccgtcg ataacgcctt ctgtcacttc acgcggcacc ccgtgagcgc
     9421 cagcagtcat cacatcagcg atactgatgc cttttcctcc ttcattccag ccaccttcca
     9481 actgatgcgc ggctaccgcg ccaccccata aaaaaccttt tttaaatcct gacatacaca
     9541 actccttaac taaaatagat taccaccaga cttc
//
LOCUS       DAJTVK010000145         9565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000145 DAJTVK010000000
VERSION     DAJTVK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.146"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            88..1344
                     /locus_tag="OO372_003635"
                     /pseudo
     CDS             88..1344
                     /locus_tag="OO372_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            1347..2675
                     /gene="ssnA"
                     /locus_tag="OO372_003636"
     CDS             1347..2675
                     /gene="ssnA"
                     /locus_tag="OO372_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCR6343028.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2726..3505
                     /locus_tag="OO372_003637"
     CDS             2726..3505
                     /locus_tag="OO372_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCR6343029.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3502..6441
                     /locus_tag="OO372_003638"
     CDS             3502..6441
                     /locus_tag="OO372_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCR6343030.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6374..6688
                     /locus_tag="OO372_003639"
     CDS             6374..6688
                     /locus_tag="OO372_003639"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCR6343031.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6724..7074
                     /gene="ghxQ"
                     /locus_tag="OO372_003640"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6724..7074
                     /gene="ghxQ"
                     /locus_tag="OO372_003640"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7143..7631)
                     /locus_tag="OO372_003641"
     CDS             complement(7143..7631)
                     /locus_tag="OO372_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6343032.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OO372_003642"
     CDS             complement(7631..9550)
                     /gene="ygfT"
                     /locus_tag="OO372_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCR6343033.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2308 a         2564 c         2491 g         2202 t
ORIGIN      
        1 gatccttaac tgatcggcat tattcctcag ttccgtattc ctgcagagtt aattcagcac
       61 gatatcgatt ttgttgccgc tcacggcgtg aaatttgagt atggctgctc acccgattta
      121 accgttgaac agttaaaaaa tcagggcttc cactatgttc tgattgccac cggcactgat
      181 aaaaatagcg gtgtgaaact ggcgggcgac aaccaaaatg tctggaaatc actccccttc
      241 ctgcgtgaat acaacaaggg tacagcgctc aagctgggca aacatgtggt cgttgtcggg
      301 gcgggtaaca ccgcaatgga ctgcgctcgt gcggcgttac gcgttccagg cgtagaaaaa
      361 gcaacgatcg tttaccgtcg ttcactacaa gagatgcccg catggcgcga agagtatgaa
      421 gaagcgttgc acgacggcgt agagttccgt ttcctgaata atccggaacg tttcgatgct
      481 gatggcacct taaccttgcg cgttatgtcg cttggcgaac cggatgagaa aggtcgtcgc
      541 cgtccggttg aaaccaacga aacagtaaca ctgcatgtag acagcctgat caccgccatt
      601 ggtgaacagc aggatactga agccctgaat gcgatgggcg tgccgctgga caaaaacggc
      661 tggccagacg tcgaccataa tggcgaaact cgtctgactg acgtctttat gatcggcgac
      721 gtacagcgcg gaccatcctc cattgtcgct gctgtcggaa ccgcgcgtcg ggcgaccgat
      781 gccatcctta gtcgggaaaa tatccgttcc caccagaacg ataaatactg gaacaacgtc
      841 aatccagcgg aaatctatca acgtaaaggc gatatctcta tcacgctggt gaacagtgac
      901 gatcgtgacg cgtttgtcgc ccaggaagcc gctcgctgcc tcgaatgtaa ctacgtttgc
      961 aacaagtgtg tggatgtctg cccgaaccgc gccaacgtct ccattgcagt ccctggcttc
     1021 cagaatagat tccagacgct gcacctcgac gcttactgta acgaatgcgg caactgcgct
     1081 cagttctgtc cgtggaacgg taaaccatac gaagacaaaa tcaccgtctt cagcctggcg
     1141 caagactttg ataacagcag caccccaggc ttccttgtgg aagattgccg ggtacgagta
     1201 cgtctgaata accaaagctg agtgttaaac atcgacagcg aaggtcagtt taacaacgta
     1261 ccaccggagc tgaacgatat gtgccgcatc atcagccatg tccaccagca tcatcattat
     1321 ctgctgggcc gcgtggaggt gtaatcatgt tgattctgaa gaatgtcacc gcagtgcagt
     1381 tacacccggc gaaagtgcag gaaggcgttg atatcgctat cgaaaatgac gtgattgtcg
     1441 ctatcggcga tgccctgacg caacgctacc ccgatgccag ctacaaagag atgcatggtc
     1501 ggattgtgat gccgggaatt gtctgctcgc acaaccattt ttactcggag ctttcccgcg
     1561 gaattatggc aaacatcgcc ccttgcccgg atttcatctc aacgctgaaa aatctctggt
     1621 ggcggctcga tcgcgccctt gatgaagagt cgctctatta cagcggactg atttgttccc
     1681 tggaagcgat taagagcgga tgtacatcgg ttatcgatca ccatgcctcg ccggcgtata
     1741 tcggcgggtc gctctccaca ttgcgcgacg catttttaaa agttggcctg cgcgcgatga
     1801 cctgttttga aactactgac cgtaacaacg gcatcaaaga gttgcaggaa ggtgtagaag
     1861 aaaacatccg tttcgcccgt ttgattgatg aggcgaagaa agcgacaagc gagccgtatc
     1921 tggtggaagc acatatcggt gctcacgcgc cgtttaccgt gccggatgcc ggtctggaga
     1981 tgctgcgtga agccgtgaaa gccacaggcc gtggtttgca tattcacgct gcggaagacc
     2041 tttacgacgt ttcctacagt caccactggt acggcaaaga cctgctggca cgactggcgc
     2101 aattcgacct catcgacagc aaaacgctgg tcgctcatgg gctgtacttg tcgaaagatg
     2161 acatcgccct actcaatcag cgcgatgcgt tcctggtgca taacgcccgt tcaaacatga
     2221 acaaccatgt cggctacaac catcacctta gcgacatccg caacctggca ttgggaacgg
     2281 acggcattgg ttcggacatg tttgaagaga tgaaatttgc cttctttaaa catcgcgatg
     2341 cgggtggtcc gctgtggcct gacagttttg ccaaagccct gactaacggt aacgaactga
     2401 tgagccgcaa ctttggcgcg aaatttgggc ttctggaagc cggttacaaa gctgatttga
     2461 ccatttgcga ttacaactcg ccgacgccgc tgctggcaga caatatcgcc gggcatatcg
     2521 ctttcggtat gggctcaggc agcgttcaca gcgtgatggt caatggtgtg atggtctatg
     2581 aagaccgtca gtttaacttc gattgcgatt ccatttatgc acaagccaga aaagccgctg
     2641 ccagtatgtg gcgtcggatg gatgcgctgg cataaatgac agatgccctc ttcccgcagg
     2701 gaagagggtt aaataaggaa agaagatgat tgaacaattt ttcaggcccg actctgtcga
     2761 acaggcgctg gaactgaagc gccgctacca ggatgaagcc gtctggttcg ccgggggcag
     2821 caaactcaat gctacaccaa cccgtaccga taaaaagatt gccatttcct tacaggatct
     2881 ggaactggac tgggttgact gggataacgg tgcactgcgg attggtgcaa tgtctcgctt
     2941 gcagccactg cgtgatgcgc gctttattcc tgcagcactg cgtgaagccc tcggttttgt
     3001 ttactcacgc catgttcgta atcagtcgac cattggtggt gaaatcgccg cccggcagga
     3061 agagtcagtg ctgcttcccg tcctgctagc actggatgct gaactggttt ttggcaacgg
     3121 cgaaacgctg tcaatcgagg actacctggc ctgcccatgc gatcgcctgc taaccgaaat
     3181 tatcattaaa gatccgtatc gcacctgtgc gacccgcaaa attagccgtt ctcaggcagg
     3241 tttaaccgtc gtgacggcag ccgttgcaat gacagaccac gacggtatgc gaattgcgct
     3301 ggatggcgtc gccagtaaag cactgcgtct gcacgatgtc gaaaaacaaa atctggaagg
     3361 caatgcactt gaacaggctg tcgccaacgc cattttcccg caggaagatt tgcggggcag
     3421 cgtggcctat aaacgctata tcacgggagt tctggtagcc gacctgtatg ccgactgcca
     3481 acaggctggg gaggaagccg tatgatcatc cactttactt taaatggcgc gcctcaggag
     3541 ctaaccgtta atccaggcga aaacgtgcaa aagctgttgt ttaacatggg aatgcactct
     3601 gtacgcaaca gtgatgatgg cttcgggttt gccggttctg acgcaataat ctttaacggt
     3661 aatatcgtta acgcgtcctt gcttattgcc gcacagttag agaaggcaga tattcgtacc
     3721 gcagaatctc tgggtaaatg gaacgagtta agtctggttc aacaggcaat ggttgatgtt
     3781 ggcgtggtgc agtctggtta taacgatcca gctgcagctc tgattatcac cgatcttctc
     3841 gatcgcatcg ccgcacctac ccgcgaagag atcgacgacg cgctttctgg tttgttcagc
     3901 cgcgatgcag gctggcaaca atactatcag gtcattgaac tggcggttgc acgtaaaaat
     3961 aatccgcagg ccaccattga tatcgctccg actttccgtg acgaccttga cgtcattggc
     4021 aagcattatc ctaaaactga tgccgcgaaa atggtgcagg cgaaaccctg ctatgttgaa
     4081 gactgcgtaa cggctgacgc ctgcgtcatt aaaatgttac gtagcccaca cgctcacgca
     4141 ctgattactc atctggatgt cagcaaagct gaagccttac cgggcgtcgt tcacgttatt
     4201 actcacctga attgccctga tatctactat accccggggg gtcagagcgc accggaaccg
     4261 tcaccgcttg accgccgtat gttcggcaag aaaatgcgtc acgtcggcga tcgtgttgct
     4321 gcggtcgtcg cagaaagtga agaaattgcg ctcgaagcac tgaagctcat cgacgttgaa
     4381 tatgaagtgc ttaagccggt aatgtcgatc gacgaagcaa tggcggaaga tgcgcctgtc
     4441 gtgcacgatg aaccggtggt gtatgttgct ggtgcgccag atactctgga agacgataac
     4501 agccatgcag cccagcgcgg cgagcatatg atcatcaact tcccgatcgg ttctcgccct
     4561 cgcaaaaata tcgccgccag tattcatggt catattggcg atatggacaa aggctttgcc
     4621 gatgccgatg tgatcattga gcgaacctat aactcaacgc aggcacagca gtgcccgact
     4681 gaaacacata tctgctttac ccggatggac ggcgatcgtc tggttatcca cgcctccacc
     4741 caggtaccat ggcacttacg ccgccaggtc gcgcgcctcg tgggcatgaa acagcataaa
     4801 gttcatgtca ttaaagagcg agttggcggc ggttttggtt ccaaacagga catcctgctg
     4861 gaagaagtgt gcgcctgggc aacctgcgtg accgggcgtc cggtactatt ccgctacacc
     4921 cgtgaagaag agtttattgc taacacctct cgtcacgtcg cgaaagtcac cgtcaaactg
     4981 ggagcgaaaa aagatggtcg cctgacggca gtgaagatgg atttccgcgc caacactggc
     5041 ccttacggca accactcact caccgtaccg tgtaacggac cggcgctgtc gctgccgtta
     5101 tatccgtgcg ataacgtcga tttccaggtc accacctact acagcaacat ttgcccaaat
     5161 ggtgcttatc agggttatgg cgcaccgaaa ggtaacttcg ctatcaccat ggcattagcg
     5221 gaactggctg aacagttaca gatcgaccaa ctggaaatta tcgaacgtaa ccgggtacac
     5281 gaagggcaag agctgaaaat tctcggtgca atcggtgaag gtaaagcgcc gacctccgtt
     5341 ccttccgccg ccagctgtgc actggaagag atcctgcgtc agggtcgcga gatgatccaa
     5401 tggtcgtcac cgaaaccgca aaatggtgac tggcacatcg gtcgcggcgt cgccattatc
     5461 atgcagaaat cgggtatccc ggatatcgat caggctaact gcatgatcaa actggaatcg
     5521 gacggcacct ttatcgttca ttctggcggt gcggatattg gtactggtct ggatactgta
     5581 gtgacgaaac tggcagcaga agtgctgcac tgcccaccgc aggacgtgca tgttatctcc
     5641 ggtgataccg atcatgcgtt gtttgataaa ggcgcatatg cctcgtccgg tacttgcttc
     5701 tcgggtaacg cggcgcgttt ggcagcggaa aatctacggg agaaaattct gttccacggc
     5761 gcgcaaatgt tgggtgaggc agtggcagat gttcaactgg caacgccggg cgtcgtgcgc
     5821 ggcaagaaag gcgaagttag tttcggggat attgcccata aaggcgaaac cggcatcggc
     5881 tttggttcac tggtgggaac tggcagttat atcacgcctg atttcgcctt cccgtatggc
     5941 gcaaacttcg ctgaagttgc cgtcaacacg cgtacgggtg aaatccgcct ggataaattc
     6001 tacgccttgc tggactgcgg tacaccggtc aacccggaat tagcattggg acaaatctac
     6061 ggtgctaccc tgcgagctat cggccacagt atgagcgaag agatcattta tgacgccgaa
     6121 ggtcacccgt taacgcgtga tttacgcagt tacggcgcac cgaaaattgg tgacattccg
     6181 cgtgatttcc gcgctgtgct ggtgccgagc gacgataaag tcggcccgtt cggggcgaaa
     6241 tcgatctcgg aaatcggtgt aaatggcgca gctccggcga ttgctaccgc aattcacgat
     6301 acatgcggca tctgggcaga agaaagtcaa agtgggcatg ggaacggata tcggtgccgg
     6361 aacaactttc aacatgctgc aaacgctgaa cgaagcctac aaagtgttgc aattacaagg
     6421 ctatcgcctc tcggcatatg aagcgtttta cctggccacg ctcggcggag cgaaatctct
     6481 gggccttgac gatttgattg gcaacttttt acctggcaaa gaggctgatt tcgtggtgat
     6541 ggaacccacc gccactccgc tacagcagct gcgctatgac aactctgttt ctttagtcga
     6601 caaattgttc gtgatgatga cgttgggcga tgaccgttcg atctaccgca cctacgttga
     6661 tggtcgtctg gtgtacgaac gcaactaata ataaaacttt aacatcctcg tgaggacatc
     6721 attatgtctg gagacatcct acaaacaccg gacgcaccaa agccacaggg cgcgctggat
     6781 aattatttta aaattaccgc tcgtggcagt accgttcgtc aggaagtact ggctggctta
     6841 acgacctttc tggccatggt ttattccgtt atcgtcgttc cgggaatgct gggcaaagca
     6901 gtttttcctc ccgcagctgt gtttgttgcc acctgtctgg tcgcgggctt cggctcgttg
     6961 ctgatggggt tgtaggccaa tttgccaatg gcgattggtt gtgcgatttc cttgacggca
     7021 tttaccgcat tcagtctggt actcgggcaa caaattagcg ttcctgtcgc actggtcgca
     7081 ttttacgcgg gtggttgggc aatctaatag attctccgcg cctttcagcg cggagatttc
     7141 tttcacagag gattctcacc gctggcaaca atacgctgct ggcggactcg cctgagttct
     7201 ctttcagtaa gcaactgcag cgcctgtgtc gggcaagatt caacacaggc tggcccctct
     7261 tcccgctgtt cacagagatc gcatttcaca atttgttgtc gggtatcccc cggcaacgac
     7321 tgaatggtga tcattccaaa cgggcacgca ctgacgcaac tctgacagcc aatacaacgg
     7381 gcagaattgg tctgcaccac ctgctcgccc atcgtcagcg cccccacggg gcaagcgcca
     7441 acacaagggg cgttttcaca ctgatggcac atcaccggcg cactgatgct gtccagtcgc
     7501 tgtaccttca gccggggtaa aaagacatcg gcattcaact cctgttctga agggtgagcg
     7561 accacacagg ctacttcaca agtacggcag ccaatacagt cagccggatt aacgataatt
     7621 aacgatttca tcacgatgcc ttcgtatcaa acagagttaa catatcgcgc gccgcctgcc
     7681 ttcctgcggc cattgcagtg acaaccagat ccgcgccatg aactgcatca ccaccagcaa
     7741 agactttttt cagatgcgtc tgggtaggta aatacccgac gtcaccggtt tgaatcaggc
     7801 cccatttatc gagtttaatt ccgctgccct gcaaccacgg catggcatgc gcctggaaac
     7861 caaaggccat aatcagaaca tcggcgggca attcaaactc tgaacccgct accggacgag
     7921 gacgacggcg accatccggc cccggctcac ccatggcggt acgaatcagg cccaccgcag
     7981 ttaaacgccc atcttcgtca caagcgatat attgcggttg aacattgaac tgaaactcga
     8041 caccttcctc gcgcgcatta accacctctt tgcgcgagcc cggcatactg acttcatcac
     8101 gacgatacgc gcaggtcacg ctggcagcat tgaggcgaat ggaagtccgc aaacaatcca
     8161 ttgtcgtatc gccaccgccc aataccacga ctcgcttacc ttccacgtcc gtcagcggat
     8221 actcttcaga ctccggcaat cccatgagct ggcgggtatg ggcagtcagg aacggtagag
     8281 cctgaatgac accgggcgca tcttcatgcg gcagatctgc tcgcatcatc ccgtaagtcc
     8341 ccacgccgat gaaaactgca tcatattcag aagttaaatc gctaaaggta atatcgcggc
     8401 caatttcaca gttaagatga aagtcgattc ccattgcggt gaatatctct cgccgctggc
     8461 ttaataccgt tttatcgagt ttgaaaggag gaatgccaaa agtcagcata ccgccaattt
     8521 ctggatggcg atcaaagaca tcaacctgaa ctcccgcgcg cgccagaata tcagcacacc
     8581 ctaaccctgc tggcccagcg ccaatcaccg ccactttttc gatacgggga acaactttgc
     8641 tgacatcggg acgccagccc atcgccagcg cggtatcggt gatgtagcgt tccagattac
     8701 cgatagagac tgcgccagag tgatctttca aagtacatgc gccttcacaa agacggtcct
     8761 gtgggcatac cctgccgcag atttctggta aggaactggt ctggtggcaa agttctgccg
     8821 cttcaataat ctttccttcc tgtaccagac ggatgtaatc cggaatagcg ttatgcagcg
     8881 ggcaatgcca gttgcagtta gctttttcgg cacaataaac acagcggtca ctctcataag
     8941 tcgcttgcag tggatccagc ccgcaataga tttcgccaaa gtgggttttc cgttcactcg
     9001 ctgaaatttt atctgcgcct ttacgcgagt taacggggag caacgctgca ctgcgagatg
     9061 gctgagcgtc tgatgacgct tttcctgctg ccgttttgcg ctggcgagcc acctttatcc
     9121 actgtaaccc tttatcgtcc atcagtcgta acgcctgtgt tgggcagact tcaatacagg
     9181 cttgcgtgcc ggaactgcgc tggttacaaa ggtcgcattt ctgcgcaatc gtatcgacca
     9241 tctcaacgac gccaaagggg caagcgattg cgcatctttt acagccaata catttttgct
     9301 cgtccagttg tacgctatcg gactggaaag tcagagcatt aaccggacaa gccgtaacac
     9361 aaggggcatt gttgcagtga tggcaggcca ccggattcgc agcctggcct ttccctacaa
     9421 cgtggatacg cggtcgaaag tcactgtgac tcagcggcca gttttcttga ttgtgtgcca
     9481 ccgcacaggc aatttcacaa gcatgacagc ctatacattc cgcagcttca gcagcgataa
     9541 acttattcat ttgcatccct ttcat
//
LOCUS       DAJTVK010000146         9501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000146 DAJTVK010000000
VERSION     DAJTVK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.147"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(149..1231)
                     /locus_tag="OO372_003643"
     CDS             complement(149..1231)
                     /locus_tag="OO372_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HCR6343034.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="OO372_003644"
     CDS             complement(1228..3024)
                     /locus_tag="OO372_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6343035.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="OO372_003645"
     CDS             complement(3275..3631)
                     /locus_tag="OO372_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343036.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="OO372_003646"
     CDS             complement(3689..4111)
                     /locus_tag="OO372_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6343037.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="OO372_003647"
     CDS             complement(4127..4852)
                     /locus_tag="OO372_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR6343038.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="OO372_003648"
     CDS             complement(4874..5620)
                     /locus_tag="OO372_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6343039.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="OO372_003649"
     CDS             complement(5627..6691)
                     /locus_tag="OO372_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR6343040.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="OO372_003650"
     CDS             complement(6763..7188)
                     /locus_tag="OO372_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6343041.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="OO372_003651"
     CDS             complement(7185..7448)
                     /locus_tag="OO372_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6343042.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="OO372_003652"
     CDS             7560..8060
                     /locus_tag="OO372_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6343043.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="OO372_003653"
     CDS             8080..8274
                     /locus_tag="OO372_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR6343044.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="OO372_003654"
     CDS             8274..8564
                     /locus_tag="OO372_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6343045.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8845..8997
                     /locus_tag="OO372_003655"
     CDS             8845..8997
                     /locus_tag="OO372_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6343046.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            8997..9374
                     /locus_tag="OO372_003656"
     CDS             8997..9374
                     /locus_tag="OO372_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343047.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT         2445 a         2492 c         2016 g         2548 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatac tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaaa taatgatgct attgtttcag gcgtcaaccc accccgcccc acagaatgca
     8701 gggcaatact tcgagttacc agacagtggt caggggttaa gtagccagcc cgaggcgtaa
     8761 gaacatgacg gcagggttca atctttaact atgcagcagg ttttttgttc cgccaccccg
     8821 gcgttaaggg gaaatgaggt cagcatggat actatcgatc ttggcaacaa cgaatctctg
     8881 gtgtgcggcg tgttccctaa ccaggatgga acgtttaccg ccatgacgta taccaaaagc
     8941 aaaacgttta aaaccgaatc tggcgcgcgt tgctggttag ccagaaacac tgactgatga
     9001 ggctgacgat ggaatttaaa gatttaccgt ctgatgtaca gaaaacagca gctcatacat
     9061 tgcattctgt gctgcgagaa atcgggaaag atattgcaag cgagccagca aaagatctgg
     9121 cccggaaaat caagaccgct ttcgttgagc tttataatgt tggcactgac tctgaaactg
     9181 tcgagaccaa gaccgtaagt tcaccaatat tctcacttga gccaagcgta ttatcaggtg
     9241 aaatatgcac cgagatttcc agcgaactgc ttccggtaat acgagaagca attcgccgtc
     9301 gcggtttgga tggaagttat gatcatgacg ttctgcaagt tctcaggaca atggtgactt
     9361 cactggggat ttgatccctg cgtcctctaa gcttttgata tagcggtcct ccaagaagcg
     9421 gtaaattttg tcgaaatcat cttttccctc tggtttattc agaggattac aggacccgta
     9481 agtagcggca tacatttcaa c
//
LOCUS       DAJTVK010000147         9432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000147 DAJTVK010000000
VERSION     DAJTVK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.148"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="OO372_003657"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="OO372_003657"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6343048.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="OO372_003658"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="OO372_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6343049.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="OO372_003659"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="OO372_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OO372_003660"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="OO372_003660"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6343050.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="OO372_003661"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="OO372_003661"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6343051.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="OO372_003662"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="OO372_003662"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6343052.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="OO372_003663"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="OO372_003663"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6343053.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="OO372_003664"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="OO372_003664"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6343054.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="OO372_003665"
     CDS             complement(6556..7449)
                     /locus_tag="OO372_003665"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6343055.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="OO372_003666"
     CDS             complement(7446..8099)
                     /locus_tag="OO372_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR6343056.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="OO372_003667"
     CDS             complement(8110..9369)
                     /locus_tag="OO372_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR6343057.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAJTVK010000148         9430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000148 DAJTVK010000000
VERSION     DAJTVK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.149"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            223..2199
                     /locus_tag="OO372_003668"
                     /pseudo
     CDS             223..2199
                     /locus_tag="OO372_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="OO372_003669"
     CDS             2192..3328
                     /locus_tag="OO372_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6343058.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="OO372_003670"
     CDS             3325..5325
                     /locus_tag="OO372_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR6343059.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="OO372_003671"
     CDS             5447..5908
                     /locus_tag="OO372_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR6343060.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALLGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="OO372_003672"
     CDS             complement(5949..6419)
                     /locus_tag="OO372_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6343061.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO372_003673"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="OO372_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR6343062.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO372_003674"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="OO372_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6343063.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="OO372_003675"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="OO372_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT         2296 a         2493 c         2417 g         2224 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg ctgggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 ccacagttac aggagggtgt tatgcgtgtt gagatctgta tcgctaaaga aaaaatcact
     9421 aaaatgccaa
//
LOCUS       DAJTVK010000149         9397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000149 DAJTVK010000000
VERSION     DAJTVK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.150"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(350..1603)
                     /locus_tag="OO372_003676"
     CDS             complement(350..1603)
                     /locus_tag="OO372_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6343064.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="OO372_003677"
     CDS             1710..2603
                     /locus_tag="OO372_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6343065.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="OO372_003678"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="OO372_003678"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6343066.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="OO372_003679"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="OO372_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6343067.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO372_003680"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="OO372_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6343068.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALSASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO372_003681"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="OO372_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6343069.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="OO372_003682"
     CDS             complement(6839..7693)
                     /locus_tag="OO372_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6343070.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO372_003683"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="OO372_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6343071.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="OO372_003684"
     CDS             complement(8323..>9397)
                     /locus_tag="OO372_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6343072.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2378 c         2439 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacaggtaca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcgga aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAJTVK010000150         9219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000150 DAJTVK010000000
VERSION     DAJTVK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.152"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            74..733
                     /locus_tag="OO372_003685"
     CDS             74..733
                     /locus_tag="OO372_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6343073.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            837..1811
                     /gene="ghrB"
                     /locus_tag="OO372_003686"
     CDS             837..1811
                     /gene="ghrB"
                     /locus_tag="OO372_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6343074.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1861..2571)
                     /locus_tag="OO372_003687"
     CDS             complement(1861..2571)
                     /locus_tag="OO372_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6343075.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3005..3295
                     /locus_tag="OO372_003688"
     CDS             3005..3295
                     /locus_tag="OO372_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR6343076.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3576..3788
                     /gene="cspA"
                     /locus_tag="OO372_003689"
     CDS             3576..3788
                     /gene="cspA"
                     /locus_tag="OO372_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6343077.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="OO372_003690"
     CDS             complement(3976..4128)
                     /gene="hokA"
                     /locus_tag="OO372_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6343078.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="OO372_003691"
     CDS             complement(4454..6523)
                     /gene="glyS"
                     /locus_tag="OO372_003691"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6343079.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="OO372_003692"
     CDS             complement(6533..7444)
                     /gene="glyQ"
                     /locus_tag="OO372_003692"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6343080.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7539..7838)
                     /locus_tag="OO372_003693"
     CDS             complement(7539..7838)
                     /locus_tag="OO372_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6343081.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8013..9008
                     /gene="wecH"
                     /locus_tag="OO372_003694"
     CDS             8013..9008
                     /gene="wecH"
                     /locus_tag="OO372_003694"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6343082.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9050..>9219)
                     /locus_tag="OO372_003695"
     CDS             complement(9050..>9219)
                     /locus_tag="OO372_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343083.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         2288 a         2331 c         2268 g         2332 t
ORIGIN      
        1 ttcatgttca cgcggcataa tctcccgcca cggaacccgt ggcaaagaat aaaaaggtta
       61 ttaaggatta acaatgaaga aacgtgttta tcttattgcc gccgtagtga gtggcgctct
      121 ggcggtatct ggctgcacaa ctaaccctta caccggcgaa cgcgaagcag gcaaatctgc
      181 tatcggcgca ggtctgggct ctctcgtggg cgcgggtatt ggtgcgctct cttcttcgaa
      241 gaaagatcgc ggtaaaggcg cgctgattgg cgcagcagca ggcgcagctc tgggcggcgg
      301 cgtgggttat tacatggatg tgcaggaagc gaagctgcgt gacaaaatgc gcggcactgg
      361 tgttagcgta acccgcagcg gggataacat tatcctcaat atgccgaaca atgtgacctt
      421 cgacagcagc agcgcgaccc tgaaaccggc aggcgctaac accctgaccg gcgtggcaat
      481 ggtgctgaca gagtatccga aaacggcggt taacgtgatt ggttataccg acagcacggg
      541 gggtcacgac ctgaacatgc gtctctccca gcagcgtgcg gattccgttg ccagcgcgtt
      601 gatcacccag ggcgtggacg ccagccgcat ccgtactcag ggccttggcc cggctaaccc
      661 aatcgccagc aacagcaccg cagaaggtaa ggcgcaaaac cgccgtgtag aaattacctt
      721 aagcccgctg taatcccttt catgccaggt gatgcagaaa tcacctggca ttcttcagtt
      781 ttccttcatc atatttcagg ctaaggtgat cgccttatca gtgaatggag agaagcatga
      841 agccgtccgt tatcctctac aaagccttac ctgatgattt actgcaacgc ctgcaagagc
      901 atttcaccgt tcaccaggtg gcaaacctca gcccacaaac cgtcgaacaa aatgcagcaa
      961 tttttgccga agctgaaggt ttactgggtt caaacgagaa tgttgatgcc gcattgctgg
     1021 aaaaaatgcc gaaactgcat gccacatcaa cgatctccgt cggctatgac aattttgatg
     1081 tcgatgcgct taccgcccga aaaattctgc tgatgcacac gccaaccgta ttaacagaaa
     1141 ccgtcgccga tacgctgatg gcgctggtgt tgtctaccgc tcgtcgggtt gtggaggtag
     1201 cagaacgggt aaaagcaggc gaatggaccg cgagcatagg cccggactgg tacggcactg
     1261 acgttcacca taaaacactg ggcattgtcg ggatgggacg gatcggtatg gcgctggcac
     1321 aacgtgcgca ctttggcttc aacatgccca tcctctataa cgcgcgccgc caccataaag
     1381 aagcagaaga acgcttcaac gcccgctact gcgatttgga tacactgtta caagagtcag
     1441 atttcgtttg cctgatcctg ccgttaactg atgagacgca tcatctgttt ggcgcagaac
     1501 aattcgccaa aatgaaatcc tccgccattt tcattaatgc cggacgtggc ccggtggttg
     1561 acgaaaatgc actgatcgca gcattacaga aaggggaaat tcacgccgcc gggcttgatg
     1621 tcttcgaaca agagccactg tccgtagatt cgccgttgct ctcaatggcc aacgtcgtcg
     1681 cagtaccgca tattggatct gccacccatg agacgcgtta tggcatggcc gcctgtgccg
     1741 tggataattt aattgatgcg ttacaaggaa aggttgagaa gaactgtgtg aatcctcacg
     1801 tcgcggacta agccgcgact gcgtgaagta aagcccgata atcgctcggg cttttactct
     1861 ttattgggtt gcagtaactg ctgtagtcca ggcctgatta aacgcctgat gttgtgccgg
     1921 taatggcgca atcagtttgt tatattcact tgcctgctgt gaagtcggga actggatgcc
     1981 atttgcgaca aagctcacct gagtaccttg ctgagcaatg tagtctccaa ccatcaccag
     2041 ttgttgcgtg aaagtttgtg ccgccggaat caacggttgc aatgcatcag ctggcgtcgt
     2101 taccactttg gtgaatgcct gatcaaatac cggtttcagg tcatcgctct gttttagcgc
     2161 agagtgcgca gcatcggcct gcaacttcgc attttgcagt tgttgcgcca gcacacccag
     2221 cgaaccattc atttcacgca gcggaccgct ttgcgtaacg taatcctgcg gtacgcgaat
     2281 tgcgttaacg ctgtctacga ccgggcgcag accagaatcc atcgcctgat tcacctgctg
     2341 agaataacca tacagaatcg cgtaatcaga gacaaaagga ccaaactgtt ttttctgatc
     2401 ggcagtcagg gttggtagac gttcaccgct acgcatcact gtattctgca ggaagtcgat
     2461 aaacgcttta cgctgatcgc cttctttatc aaaacaccca ctcaggctaa ctaccattaa
     2521 taacgccgca agaggcgcaa accagcgaga gcaggacttt cctgtcgcca ttttcttact
     2581 cctttcaccc aaaaaaagcg cacaacgaca cacgcgtgtc tgacgtgaca aggatagtcc
     2641 aggacaggct tgcaggatac cctttctacg taatcttcct ggtaaaatca tggagaaaat
     2701 gaaacaaggt taatttgttg tttcattgtt aaaaaatgat agtgccgttg ccttttttgt
     2761 agccagagat ttacgccttc gcagaaggtt agattttcga aatctttaca aaacaggagt
     2821 ggtaagagat atatcggcat ttatcagatc gttaggtggc tataagtcac gctaaatgac
     2881 aggctgaatc gaatcatagc cagagcatgc cctgacttca ccccgctgtg tctgcttttc
     2941 ccgactattc ttaatgagct tcgatgcaat tcacgatccc gcagtgtgat ttgaggagtt
     3001 ttcaatggaa tataaagatc caatgcatga gctgttgagc agcctggaac agattgtttt
     3061 taaagatgaa acgcagaaaa ttaccctgac gcacagaaca acgtcctgta ccgaaattga
     3121 gcagttacga aaagggacag ggttaaaaat cgatgatttc gcccgggttt tgggcgtatc
     3181 agtcgccatg gtaaaggaat gggaatccag acgcgtgaag ccttcaagtg ccgaactaaa
     3241 attgatgcgt ttgattcaag ccaacccggc attaagtaag cagttgatgg aatagacttt
     3301 tatccacttt attgctgttt acggtcctga tgacaggacc gttttccaac cgattaatca
     3361 taaatatgaa aaataattgt tgcatcaccc gccaatgcgt ggcttaatgc acatcaacgg
     3421 tttgacgtac agaccattaa agcagtgtag taaggcaagt cccttcaaga gttatcgttg
     3481 atacccctcg tagtgcacat tcctttaacg cttcaaaatc tgtaaagcac gccatatcgc
     3541 cgaaaggcac acttaattat taaaggtaat acactatgtc cggtaaaatg actggtatcg
     3601 taaaatggtt caacgctgac aaaggcttcg gcttcatcac tcctgacgat ggctctaaag
     3661 atgtgttcgt acacttctct gctatccaga acgatggtta caaatctctg gacgaaggtc
     3721 agaaagtgtc cttcaccatc gaaagcggcg ctaaaggccc ggcagctggt aacgtaacca
     3781 gcctgtaatc tctgcttaaa agcacagaat ctaagatccc tgccatttgg cggggatttt
     3841 tttatttgtt tttcaggaaa taaataatcg atcgcgtaat aaaatttagt attatttttg
     3901 tgaagaataa atttgggtgc aatgagaatg cgcaacgccg taagaaaggc gggaataact
     3961 tcccgccaaa gactcttact ctttcaaatt gcaggctaaa aatgccgcca gctcataact
     4021 ctcctgttta atatgcaatt cacacagtga atctcttatc atccaggtga aaaataaaag
     4081 cgtgaaacaa atcactatta aagaaagtaa tctatatttc tgcggcattc ccagcctcct
     4141 gtgttgattt ccaacgagta agaggctaac cttatggtgt tcaggcatag ggtagcctca
     4201 ctttgattta tagtcaggtg gggcttttct ctgtctgcct ttcggtgaat acctgagaca
     4261 aacagtctca agcacccgtg gctattctag cttaataagt ttgtttcttc tccttgatat
     4321 aatcctaaaa aaacctcata aaattaatat atgagataat ctttattcaa cagaagatta
     4381 ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg caggcttttt
     4441 aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca
     4501 gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc atgaccatca
     4561 ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc agcgcatcct
     4621 ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca acctgcatcg
     4681 ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg ctcagcactt
     4741 cgtcagattt cgccagaatg ttagatacac gtttgttcgc cgccgccagt gcagcagctg
     4801 catccagggt acggaaatgc gataccgctt tcatacgggc atcgaaatca gccggacgag
     4861 tcggacgacg cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg tcctgatacc
     4921 aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aactacgttg gcgttagtca
     4981 gcttatcgcc atacagacgc actgcttctt cggtcagcgt ttgcagatca aggttgaggt
     5041 tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca aacgggtctt
     5101 tgtcgccttt cggatgctga ccgataccga agatacccgc cagagtatcc atcttatcag
     5161 caatcgccag cgcacaagct accgggttgg acggcaggtc atcaccggca aagcgcggct
     5221 gatactgctc attcagcgcg acggctacgt cttccgcttc gccatcgtga cgcgcgtagt
     5281 gcatccccat aacgccttgg gtgtcggtga actcgaagac catgttggtc atcaggtcac
     5341 acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa
     5401 tccagccagc cagcgcctgg atgcggtcag ttttgtcacg cagcgtaccc agctgttgct
     5461 ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgtttttta cggtcggtat
     5521 tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta ccagagataa
     5581 tttgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc agcagtttgc
     5641 catcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa accagggctt
     5701 cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc ggccactcca
     5761 ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg ccaatcttac
     5821 gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa tcggcgatga
     5881 ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg gtgaattccg
     5941 gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg
     6001 ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc acgaagtgca
     6061 cgtcgcttgc gccccaacgc atcagtttcg ggatcggcag tttcgccagc gaagtcgcaa
     6121 ccatattcgg cagtagtgct tcagtgcttt cgcccttcac atgggcgcga tacagcagcc
     6181 attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaataccg caaccacgcg
     6241 cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg
     6301 ggccgcgttt ttcgatttca cgatctggtt gcgcttccgc caggttagct actttcaacg
     6361 ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca
     6421 gctccgcagt aaagttcgca gcaaaggact cagccaggct gcgcagtgct tttggtggca
     6481 gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc tcttacttat
     6541 ctttgttgca catcgggaag ccgagggctt cacgggaagc gtagtatgct tctgccactg
     6601 ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcagtgacg gagatggctt
     6661 tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg taggctggca
     6721 gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac tgctcgaagc
     6781 aggtgaacag gaaatccaca tccgcgtatt cgaagttgta agtggactgc tccacttcgt
     6841 tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc cagaccaggt
     6901 cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg taggtgatct
     6961 cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg aactgcgtca
     7021 cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccaga gtcgggtttt
     7081 cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc agctctttca
     7141 gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc acctggaact
     7201 gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc gggcgacgag
     7261 aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga caggtcattg
     7321 ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg cagccctggc
     7381 gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta tcaaactttt
     7441 gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta tacccgctgg
     7501 atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga aagatgcaaa
     7561 aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt ataaccgcgc
     7621 atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg
     7681 ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga acgcagagga
     7741 tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaccaacc cactagcacc
     7801 ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc tggtttgcag
     7861 gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc cttaagcctc
     7921 tataagctgt agtgataaaa ctcagattat tccagtgcgc caaccaaatg atttatcggt
     7981 cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac tggattgata
     8041 acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc tggtatgtga
     8101 ccaatgctca tagtgttagc cccgtcacat gggatatcgc caatgttctg aattctgcct
     8161 ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt ggcgaacgca
     8221 gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat agcgcaatcg
     8281 cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg aaaaacctgc
     8341 tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg atttatctgg
     8401 tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg
     8461 tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt tttgaatggc
     8521 tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat ggcatgttgg
     8581 gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg agcgccgcgc
     8641 tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg cagtggcgcg
     8701 gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc tgcgcaatcg
     8761 cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga cttggcttaa
     8821 tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat gcgctgcgca
     8881 cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc ttttgcgcga
     8941 cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga aacagattag
     9001 tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat tattcgctgt
     9061 attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggcatca cgaatattct
     9121 tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa aaaccttttt
     9181 cactgagtaa taatgtcgca ttccacagac ctaccatca
//
LOCUS       DAJTVK010000151         9188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000151 DAJTVK010000000
VERSION     DAJTVK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.153"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="OO372_003696"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="OO372_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="OO372_003697"
     CDS             complement(1751..2509)
                     /locus_tag="OO372_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6343084.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OO372_003698"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="OO372_003698"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6343085.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OO372_003699"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="OO372_003699"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6343086.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="OO372_003700"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="OO372_003700"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6343087.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="OO372_003701"
     CDS             complement(5474..6079)
                     /locus_tag="OO372_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343088.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="OO372_003702"
     CDS             complement(6082..6849)
                     /locus_tag="OO372_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343089.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="OO372_003703"
     CDS             complement(6852..7562)
                     /locus_tag="OO372_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343090.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="OO372_003704"
     CDS             complement(7578..9083)
                     /locus_tag="OO372_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6343091.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2359 c         2262 g         2195 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAJTVK010000152         8840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000152 DAJTVK010000000
VERSION     DAJTVK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.154"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            814..1056
                     /locus_tag="OO372_003705"
     CDS             814..1056
                     /locus_tag="OO372_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343092.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OO372_003706"
     CDS             complement(1386..2315)
                     /gene="fdhE"
                     /locus_tag="OO372_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6343093.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OO372_003707"
     CDS             complement(2312..2947)
                     /gene="fdoI"
                     /locus_tag="OO372_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6343094.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OO372_003708"
     CDS             complement(2944..3846)
                     /gene="fdoH"
                     /locus_tag="OO372_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6343095.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OO372_003709"
     CDS             complement(3859..6909)
                     /gene="fdnG"
                     /locus_tag="OO372_003709"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6322..6324),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6343096.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7103..7936
                     /gene="fdhD"
                     /locus_tag="OO372_003710"
     CDS             7103..7936
                     /gene="fdhD"
                     /locus_tag="OO372_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6343097.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            8089..>8840
                     /locus_tag="OO372_003711"
     CDS             8089..>8840
                     /locus_tag="OO372_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR6343098.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT         2121 a         2233 c         2265 g         2221 t
ORIGIN      
        1 tcatttacat cattccctgt ctatgccggt aatattgaat aagaactcta taaattttta
       61 ttcttcgttg gcaccatacg tgtcttgcta catcactgtt caggagcatt taaatattat
      121 ttgtagagct gagtggcaat tatagcagat aatgcttaac tcatcatctt agttcaggtt
      181 tgagcgattt ttgatagtca aaacctcctc tggtgccata cttttctcat aaatattttt
      241 aaaatctata gctcacaaac attatgcaaa gataatgggc tggcatcatt gtcagtatca
      301 ttcatttact gctctgtatg ttatgaatca ttatgttgta tgtttgctct taaaccgtgg
      361 gaaggaggct ataatggttg ccaatattaa tctcattaaa caagaaagtt attctgttgt
      421 taatttagaa aaacaacttt cccgaactgt taccaacaaa attataacga tgagcaagta
      481 aagaccggtt ggtggcagta ctgaccggtc tgtgatgaaa gattaatttc accttaaatc
      541 ctggtgctgt taggatgctg acgtttttcg gctacaccat tcagattatg caaataaata
      601 tccctgaata taatatattc atagcaccca taactcatcc tgggcgataa ctaaaccatt
      661 tttgagtaat aaatgaaaca actgcaacgc tcttattatt tctggaaaac gcctcgcaaa
      721 aataaaaaat gatgcgtaaa tgagctttct ttaaagattc ggcaagggta cttttctctc
      781 catagattca aaaatgatat tggcgagata tttatgaact ctctggcagg tatagatatg
      841 ggcagaattt tactcgattt atcgaatgag gtgattaagc aacttgatga tctcgaggtg
      901 cagcgtaatc ttcctcgtgc ggacctatta agggaagcag tagatcaata cctgataaat
      961 caatcgcaaa cagcaagaac cagtgttcct ggcatctggc aagggtgtga ggaagatggt
     1021 gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg aaaaaatgat gtcaatcaga
     1081 ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
     1141 aaagtcatgc aaattcaata tattgaagtt tattggtagg cctgataagc gcagcgcatc
     1201 aggcaatcat acgcttgtca tcagtgacat acccataacc ttatcgatgt gttggaggga
     1261 agaacgaggc aaacagacaa ttgcggcatg atcttgaatg ggtaacgcgg aatacgagag
     1321 atggtgtcgt cacaccctat gagctgtaat ccgggcgacc tgcgccaccc ggaaaacgtc
     1381 agagattact ctccttcacc cggaaacagg aacgggttaa tggaactacg ggcgtagcct
     1441 tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt catctgccac ggcttcaact
     1501 ttcggttctt tttcctgata aagaatcttc aggtaagtgc cgcagtcatc gcagctttcg
     1561 gctttaatcg cggcctgttc gtcatccagc gaccagtaat gcagtttgcc gctctgctcg
     1621 cagttgctgc attttacgcg cactacgtgc cattcggttt cacacaggtt gcagtgcagg
     1681 taacgcagac cctgagtggt gccaatttgc accatgctgg acaccggcat agagccacat
     1741 accgggcaat attgacgttg ttcgccgtat tcagcgcggg ctttgccggg gatcagattg
     1801 gccatctgcg cccagtagag cgacagcgca gcccagataa acggcgcttt atcgctgctg
     1861 acggacgaga aatcagaggc aaacagcgcg ctggccatat cttccagctc ctgagtcgat
     1921 gccttctcca gattctcaat cactgccagc gccgggccgc tcatttcagg tttcagctca
     1981 gcaattagcg ccatcagcag cttttgccag tgcttatcac gcggcagaac gtgaatatcc
     2041 agcgggggct tgccctgtgc gctggcttct ttaatgcgcg cggtcagatc catctccagc
     2101 ggatggtcgt acagcaccac ttcctgggcg tgggcgataa gcgcagcaaa gcgcaggtaa
     2161 tcacccagcg gattattttc tgccagctcg cgcagacgct cggcgcggcg gttgtataaa
     2221 ttcttgagcc gagggaacaa taacggcgga atcatatccg ccgtacgttt ctcgctcgaa
     2281 cccagctcat cttgcgggat tatgcgaata ctcattcagc tttcttttcc gttgtcttgc
     2341 ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca ggcgctggta acccatcctt
     2401 ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc gtagatatgc accatgataa
     2461 ccacaattaa cgccactgcg gcaaatgaat gcagcattaa cgcgaatcgg atcaccggga
     2521 ttgagaaagc aggcgcaaaa taaggacgcc agataatcac gccgctcacc agcagcagaa
     2581 ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc gaagttataa cgcccggtgt
     2641 cacctacttc ctcgttgacg acgatcttac gaatattctt cgcccaaaag atatcatccc
     2701 gattgattag gttgtgatgc cagtaacgga aaaacatgat gatgaacgag gcaaacataa
     2761 ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg tgtgcccatg atttgcatca
     2821 accagttgaa ggacgggaac aaaaagccca gcccgctcac cgccgccagg atgaagcaga
     2881 aggcggtgat ccagtggttg atacgttccg gcgcggtgta gcgcacgatg gtgtcacgtc
     2941 gtttcatttg cgctcctcgt ctttctcttc gtgcagatta ttctcttcct catccgcacg
     3001 gttcggaccg acacccacgt agtggaagat actggccgca aaggtagccg caaagccaac
     3061 agccgcgagc ggtttccaga tgcctttcca gaatttcacg gtttcgctga tttccgggtt
     3121 ctccggcaag ccatgataca gatttggctt gtcagcatgg tgcagcacgt acatgacgtg
     3181 tgtaccaccg acgcctgccg gatcgtacag acccgcattg tcgtaaccgc gggttttcag
     3241 ctcagcaacg cgctcgctcg ccagcgtttt catcgactct ttcgtaccga agtgaatcgc
     3301 gcccgttggg caggtcttca cgcaggctgg ttcttgccca accaccacgc ggtcaacgca
     3361 cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg cgcggaatgt cgaacggaca
     3421 gcccgcaatg caataaccgc agccaatgca ctgctcggac tggaagtcga cgataccgtt
     3481 ggcatactga ataattgcac cttccgccgg gcacgctttc aggcagcctg gatcggaaca
     3541 gtgcatacag ccgtctttgc ggatcagcca ttccagtttg tcgttctgct ccacttccga
     3601 gaagcgcatc accgtccacg atttggcgct taaatcattg gggttgtcgt acaccccaat
     3661 gttattgccg acggtatcgc gaatgtcgtt ccactctgaa cacgccacct gacaggcttt
     3721 acagccgata caggtggtga cgtcgatgag tttcgccact tcttcctgga agtcccgcgc
     3781 ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga atgatatctt gcgattgata
     3841 agccatatgt cgtctccgtt acaccttttc cacattcaca aggaaggact taaactccgg
     3901 cgtctgcgtg ttcgcatcac caacgaatgg cgttaacgta ttggcaataa agcctttttt
     3961 agctacgcct tcatagcccc agtgaatcgg aataccgatg gtatcgatat ctttgccgtt
     4021 cgctttcagc gtgcgaatac gtttggtcac caccgctttg gctttgatat agccacggtt
     4081 ggaggagact ttcacggtat cgccctgggc aatgccaagt ttattcgcca gcgactcccc
     4141 gatttccaca aactgctctg gttgcaaaat cgcgttcaac agcgcgtgtt tggtccagta
     4201 gtggaagtgc tcggtcagac gataggtggt tccgacatac gggaacttat cggctttacc
     4261 caatgcttcg gcgtcgtctt taaagattcg cgcagccggg ttcgagataa cgtttggatg
     4321 cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag tgttccggga acggaccttc
     4381 cgccatctta tcgagggcaa acagacgccc catgccttcc tgctgcatga taaacggccc
     4441 gacgccgctg cccggaggcg ctgcgctgta gtccggaata tcccagccgg tccacttagt
     4501 gccgtcccat ttcagcaact gacgcttcgg atcccacggg ttaccctgcg gatctgcgga
     4561 ggcgcggtta tacagaatgc ggcggttaag cggccatgcc catgcccagc ccagcgtgtt
     4621 accgaggcca gacggatcgg cgttatcacg acgcgccatc tggttgcctt ccggcgtcca
     4681 gctaccggcg aaaatccagc agccacagga ggttgtaccg tcatcgcgca attgggcgaa
     4741 cgagctaagt tgttggcctt tcttgacgat aaccgcaccg gttgccggat cggtaatatc
     4801 ggccagcgcc ttaccgttgc tctccatcgc cacttcttcc gacttcggct catgcggaat
     4861 ggcgtagttc caggtcatgt tcagcacctg atccgggttt gcgccaccct gttcggcata
     4921 catcttgcgc aagcgcagga agataccgga gaggatctcg ccatcagtca gcgcaatccc
     4981 tggggcgtcc gcacctttcc agtgccactg caaccagcga cctgagttaa cgattgaacc
     5041 gttctcttct gcgaagcagg tcgatggcag acggaacact tcggtctgga tcttcgacga
     5101 gtcaacttcg ttcagctcac cgtggttctg ccagaagtta gaggtttcag tgttcagcgg
     5161 gtcgatggtg acgaggaact tcagtttcga cagacaaccg atcactttgt ttttgttcgg
     5221 gaatgaggca acagggttaa agccctggca gatatagcca ttgaccttgc cctctttcat
     5281 catctcgaag tattgcagga cgtcgtagcc tttatcccac ttcggcaacc agtcaaagcc
     5341 ccagctattt tccgccgtcg ctttatcacc aaagaaggcc ttcatcatag agacgaagaa
     5401 tttcgggtag ttgccccagt agtttacctg gccttccagc agcggttttg gcgtgttggc
     5461 ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc agcgtcatgt aacctggcag
     5521 gctctgcgac agcagcccca ggtccgtcag cccctgaata ttggagtgac cgcgcagggc
     5581 gttaacgccg ccgcctgcca tccccatatt gccgagcagc agctggatca tcgccatcgt
     5641 acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg agggcataca ggaacgaggc
     5701 agttttatcg tgagcactgg tttctgcgat gtattcgcag actttcagga acgcgtcttt
     5761 tggcgtacca cagatgtttt caaccacatc tggcgtgtag cgggaaacgt gctgtttcag
     5821 caagttccac acgcagcgcg gatgttgcag cgtggtatcg cgtttggcga agccgttttc
     5881 gtccagttcg taagtccagg aggatttatc gtacttgcgt ttttccgcgt catagccggt
     5941 gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc aggctggcgt tggtataggc
     6001 ttcggtgtat tcgcggttga atttttcatt gttcagcagg tacagcaata cgcctgacag
     6061 gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag tcagccaccg ccgccgtacg
     6121 cgtaaagcga ggatcgatca caatcagctt cgcgccattg tgaattttgg cttccatcgc
     6181 ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg cccatcacca cgacgaggtt
     6241 ggcgttcttg atgtcgaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag
     6301 acttgctacc gttggtccgt gtcagacacg cgcctggttg tcgaccgcga gcatacccag
     6361 cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta ctcgacgcgg aagcacacag
     6421 catcccggtg gagagccagc ggttaacagt cacgccttcg gcgttttgcg caatgtagtt
     6481 agcatcgcgg tcttctttca tcagtttggc gatgcgatca aacgcctctt cccaactgat
     6541 ttgctgccat ttatcagaac ctggcgcacg gtattccgga aacttcagac ggctttcgga
     6601 gtggatgaaa tccaccaggc cagcgccttt cggacaaagt gcaccgcggt tgaccgggtg
     6661 atccggatcg ccttcgatat ggaagataga tgctttggcg ttttttgctc cgtcaccgag
     6721 gctgtacatc aacagcccac agcctacgga acaataggtg caggtattac gggtttcgcg
     6781 ggtgcgcagc agtttatact gccgggtttc cgcgagtgct acgctgggtg caaaacccag
     6841 tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc ttaaagaact gccttctgct
     6901 gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc ccatttacat tcgcttgctg
     6961 cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg gccgcaaagg tccagaattg
     7021 gctcaatttc cctccatcca ggaagggttt gtaacagaat accataatgt tggtgtgtgt
     7081 gttcttatct ggttaagaga aagtgaaaaa aacacagcga aaagaaatcg aaaatgtgac
     7141 aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt gatgatttac aacacccacg
     7201 gttagatgag gtcgcggaag aagttcccgt tgcgctggtc tacaacggca tttcgcatgt
     7261 ggtgatgatg gcgtcgccca aagaccttga gtactttgcg ctcggttttt cgctttccga
     7321 ggggataata gaaagtccgc gcgatatctt cggcatggat gtcgttcctt cctgtaatgg
     7381 tcttgaagta caaattgagc tttccagccg ccgctttatg gggttgaagg agcgccgccg
     7441 ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg gagcaactta atgacatcgg
     7501 aaaaccggtg cagccgctac cgttcaccca gacgtttgat ctcaacaaac tggatgatgc
     7561 attacgtcat ctcaacgatt ttcagccagt ggggcaactg actggttgta ctcacgccgc
     7621 tgcctggatg ttgccatctg gcgaactggt cggcgggcat gaagacgtgg gtcgccatgt
     7681 ggcgctggac aaactgttag gccgccggtc acaagaaggg gaaagctggc agcaaggtgc
     7741 ggtactggtt tccagtcgtg ccagttatga aatggtgcaa aagtcggcga tgtgcggcgt
     7801 agagattttg tttgcggtgt ctgccgcgac cacgcttgct gtagaagtgg ctgagcgctg
     7861 taatctgaca ctggtaggtt tttgtaaacc gggtcgggca acggtttata cccatccgca
     7921 gcgtttgagc aattaattaa aataagtcct gcgatatata ttaaattgca ggacttattc
     7981 atttcgtgaa ttttattatt ttatttataa ataaaacgcg tgtagtgacg ttttcttttt
     8041 tcatttatta tgttcactct cttatctata tatttataag gcaattaaat gaaaaggaat
     8101 ttattatctt ccgctattat agtcgccatc atggccctcg gtctgacggg ttgtgatgat
     8161 aaaaaagccg aaacagaaac gcccccgcct gccaatagtc aacctgccgc accagctcct
     8221 gaagctcccg ttgcaaaagc agaagctaaa cctgaaacac ctgcgcaacc ggtggtcgat
     8281 gaacaagcgg ttttcgacga aaaaatggat gtctatatca agtgctacaa caagttacag
     8341 atccctgtac agaacagtct ggcgcgttat gctgactggc tgaaagattt taaacagggg
     8401 cctaccggta aagagagtac tgtttatggc atctacggca ttagtgaatc caacctcgct
     8461 gagtgtgaaa aaggcgtaaa aagtgctgtg gcgttaacgc ctgcgctgca acctattgat
     8521 ggcgtggcgg tgagttatat tgatgctgcc gtggcgttgg gtaataccat taacgaaatg
     8581 gataaatatt acacccagga aaattataaa gacgatgcgt ttgcgaaagg gaaaacgctg
     8641 caccagacat tcttaaaaaa tctggaatcc tttgaacctg tagcagaatc ttatcatgcg
     8701 gcgattcagg aaattaatga taagcgccag cttgcggaac tgaaaaatat tgaagaaaga
     8761 gaagggaaaa cattccacta ctactctctg gcagtcatga tttcagcgaa acaaattaat
     8821 aacctgattt cgcaagagaa
//
LOCUS       DAJTVK010000153         8810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000153 DAJTVK010000000
VERSION     DAJTVK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.155"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..766)
                     /locus_tag="OO372_003712"
                     /pseudo
     CDS             complement(1..766)
                     /locus_tag="OO372_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1045..1998
                     /gene="tal"
                     /locus_tag="OO372_003713"
     CDS             1045..1998
                     /gene="tal"
                     /locus_tag="OO372_003713"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6343099.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2113..2700
                     /gene="mog"
                     /locus_tag="OO372_003714"
     CDS             2113..2700
                     /gene="mog"
                     /locus_tag="OO372_003714"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6343100.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO372_003715"
     CDS             complement(2735..3301)
                     /gene="satP"
                     /locus_tag="OO372_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6343101.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO372_003716"
     CDS             complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="OO372_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6343102.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4189..4593)
                     /locus_tag="OO372_003717"
     CDS             complement(4189..4593)
                     /locus_tag="OO372_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6343103.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4970..6886
                     /gene="dnaK"
                     /locus_tag="OO372_003718"
     CDS             4970..6886
                     /gene="dnaK"
                     /locus_tag="OO372_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6343104.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO372_003719"
     CDS             6975..8105
                     /gene="dnaJ"
                     /locus_tag="OO372_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6343105.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO372_003720"
     CDS             complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="OO372_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6343106.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2333 a         2187 c         2259 g         2031 t
ORIGIN      
        1 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
       61 gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat gctggtcagc
      121 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
      181 ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac cagcggggga
      241 aaatcaaaag aaaactcagg gctcgggcaa cggcgttcgc ctgaactccg ctgaaaatta
      301 tgccgtaggc gatgagcaaa aagacggcga acagaacgcc catccaacgc atccccagcc
      361 cgcgcgccat ataccatgcc ggtcctccac gaaactgtcc attgacgtca cgttctttat
      421 aaagttgtgc aagggaacat tcggcaaacg aggtcgccat gccgataaac gcggcaaccc
      481 acatccaaaa gacggctcca ggtccaccgg cggtaatagc cagcgcaacg ccggccaggt
      541 tgccgctacc cacgcgcgcc gcaagactgg tacacaatga ctgaaatgag gttaaaccgc
      601 ctggctgtgg atgaatgcta tttttaagac ttttgccaaa ctggcggatg tagcgaaact
      661 gcacaaatcc ggtgcgaaaa gtgaaccaac aacctgcgcc gaagagcagg taaatcatta
      721 ccgatcccca aaggacgctg ttaataaagg agaaaaaatc tggcatgcat atccctctta
      781 ttgccggtcg cgatgacttt cctgtgtaaa cgttaccaat tgtttaagaa gtatatacgc
      841 tacgaggtac ttgataactt ctgcgtagca tacatgaggt tttgtataaa aatggcgggc
      901 gatatcaacg cagtgtcaga aatccgaaac agtctcgcct ggcgataacc gtcttgtcgg
      961 cggttgcgct gacgttgcgt cgtgatatca tcagggcaga ccggttacat ccccctaaca
     1021 agctgtttaa agagaaatac tatcatgacg gacaaattga cctcccttcg tcagtacacc
     1081 accgtagtgg ccgacactgg ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc
     1141 acaaccaacc cttctctcat tcttaacgca gcgcagattc cggaataccg taagttgatt
     1201 gatgatgctg tcgcctgggc gaaacagcag agcaacgatc gcgcgcagca gatcgtggac
     1261 gcgaccgaca aactggcagt aaatattggt ctggaaatcc tgaaactggt tccgggccgt
     1321 atctcaactg aagttgatgc gcgtctttcc tatgacaccg aagcgtcaat tgcgaaagca
     1381 aaacgcctga tcaaactcta caacgatgct ggtattagca acgatcgtat tctgatcaaa
     1441 ctggcttcta cttggcaggg tatccgtgct gcagaacagc tggaaaaaga aggcatcaac
     1501 tgtaacctga ccctgctgtt ctccttcgct caggctcgtg cttgtgcgga agcaggcgtg
     1561 ttcctgatct cgccatttgt tggccgtatt cttgactggt acaaagcgaa tactgataag
     1621 aaagagtacg ctccggcaga agatccgggc gtggtttctg tatctgaaat ctaccagtac
     1681 tacaaagagc acggttatga aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa
     1741 attctggaac tggcaggctg cgaccgtctt accatcgcac cggcactgct gaaagagctg
     1801 gctgagagcg aaggggctat cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt
     1861 ccggcgcgta tcactgagtc cgagttcctg tggcagcaca accaggatcc aatggcagta
     1921 gataaactgg cggaaggtat ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg
     1981 atcggcgatc tgctgtaatc attcttagcg tgaccgggaa gtcggtcacg ctacctcttc
     2041 tgaagcctgt ctgtcactcc cttcgcagtg tatcattctg tttaacgaga ctgtttaaac
     2101 ggaaaaatct tgatgaatac tttacgtatt ggcttagttt ccatctctga tcgcgcatcc
     2161 agcggcgttt atcaggataa aggcatccct gcgctggaag aatggctgac atcggcgcta
     2221 accacgccgt ttgaactgga aacccgctta atccccgatg agcaggcgat catcgagcaa
     2281 acgttgtgtg agctagtgga tgaaatgagt tgccatctgg tgctcaccac gggcggaact
     2341 ggcccggcgc gtcgtgacgt aacgcccgat gcgacgctgg cagtagcgga ccgcgagatg
     2401 cctggctttg gtgaacagat gcgccagata agcctgcatt ttgtaccaac tgcgatcctt
     2461 tcgcgtcagg ttggtgtgat tcgcaaacag gcgctgatcc ttaacttacc cggtcagccg
     2521 aagtctatta aagagacgct ggaaggtgtg aaggacgctg cgggtaacgt tgtggtacac
     2581 ggtatttttg ccagcgtacc gtactgcatt cagttgctgg aagggccata cgttgaaacg
     2641 gcaccagaag tggttgctgc attcagaccg aagagtgcaa gacgcgacgt tagcgaataa
     2701 aaaaatcccc ccgagcgggg ggatctcaaa acaattagtg ggattcacca atcggcagaa
     2761 cggtgcgacc aaactgctcg ttcagtactt cacccatcgc cagatagatt gcactggcac
     2821 cacagatcag accaatccag ccggcaaagt ggatgattgc ggcgttaccg gcaatgttac
     2881 cgatcgccag cagggcaaac agcacagtca ggctaaagaa aacgaattgc agaacgcgtg
     2941 cgcctttcag cgtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga
     3001 caccaaggaa ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg
     3061 caaccagcgt caaccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc
     3121 cttttttgta ctccagcaga ccggcaaaaa tttgcgcgat gccgccgtag aaaatgccca
     3181 tggcaagaat aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg
     3241 tggtcatgcc gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca
     3301 taattcctca aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taacagtagt
     3361 gttttcaggg cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg
     3421 aaaatgaaaa ttttccccgg tttacggtgt cagacctgag tggcgctaac catccggcgc
     3481 aggcaggcga tttgtattac ggctggaatt gtcacgcgat aggcactgcc gctgaccgct
     3541 ttaaccccat ttaatgctgc gcccacaggg cctcccagac ccgcgccgcg cagcaaacca
     3601 tgcccaagca cgctcattgc tgcgtgggtg cgtaaaatgc gggtcaattg gctggaaagc
     3661 aaatgcgaca cgccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc
     3721 agctcattca ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccata
     3781 ttcttccagg tgttacgcag aaattgttcc agtaactgtt gctcaatctc aaacgtagac
     3841 atctctttgt cggctttcag cttcaatcgt tttgaaacat cgagcaaaat ggcccgatac
     3901 aatttaccgt gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct
     3961 ccagcaatca gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac
     4021 agttcgttgc gcatcagtac gctggagagg cgagttttgc ctttttcatt atgggtgagc
     4081 aatcgggcga aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa
     4141 tcattcaggt aattaacatt cattttttgt ggtttctata ttctggcgtt agtcgtcgcc
     4201 gataattttc agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt
     4261 tgagacgcag cgtttattgt cgttatcact gttaatgttg atccagtcag tggtttgccc
     4321 ttcttttatt tctgaaggaa tattcagact ctgactggcg ctacgggcgg ctttgaaata
     4381 aaccgatgca ccgcttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac
     4441 aatgcgacaa acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat
     4501 gacgccgagg agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc
     4561 caggctggcg gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag
     4621 agggatgaat acccactagt ttactgctga taaagagaag attcaggtac gtaatctttt
     4681 ctttttatta caattttttg atgaatgcct tggctgcgat tcattcttta tatgaataaa
     4741 attgctgtca attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa
     4801 aaaatttttg catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc
     4861 agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat
     4921 tacagactca caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat
     4981 aattggtatc gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccactcc
     5041 tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct tctattattg cctataccca
     5101 ggatggtgaa actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa
     5161 caccctgttt gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg
     5221 tgatgtttcc atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga
     5281 agttaaaggc cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa
     5341 gaaaaccgct gaagattact tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc
     5401 atactttaac gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga
     5461 agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg gcttacggtc tggacaaagg
     5521 tactggcaac cgtactatcg cggtttatga cctgggtggt ggtactttcg atatttctat
     5581 tatcgaaatc gacgaagttg acggcgaaaa aaccttcgaa gttctggcta ccaacggtga
     5641 tacccacctg gggggtgaag acttcgacag ccgtctgatc aactacctgg ttgaagaatt
     5701 caagaaagat cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga
     5761 agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc
     5821 gtacatcact gcagatgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa
     5881 actggaaagc ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact
     5941 gcaggacgct ggcctgtccg tatctgatat cgacgacgtt atcctcgtgg gtggtcagac
     6001 tcgtatgcca atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga
     6061 cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg
     6121 tgacgtaaaa gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat
     6181 gggcggtgtg atgacgacgc tgatcgcgaa aaataccact atcccgacca agcacagcca
     6241 ggtgttctct accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga
     6301 acgtaaacgt gcagctgata acaaatctct gggtcagttc aacctggatg gtatcaaccc
     6361 ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatctt
     6421 gcacgtttcc gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc
     6481 ttctggtctg aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga
     6541 agctgaccgt aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca
     6601 cagcacccgt aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc
     6661 tatcgagtct gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat
     6721 cgaagcgaaa atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca
     6781 gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga
     6841 cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaacg
     6901 ggtaattata ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg
     6961 acaatttaaa aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag
     7021 cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc catgaaatac cacccggacc
     7081 gtaaccaggg tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag
     7141 ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc
     7201 aaggtggcat gggcggcggc ggttttggcg gcggcgcaga cttcagcgat atttttggtg
     7261 acgttttcgg cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg
     7321 atttacgcta taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga
     7381 tccgcattcc gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta
     7441 cacagccgca gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat
     7501 ttttcgctgt acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc
     7561 cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa
     7621 tcccggcagg ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg
     7681 agcatggcgc accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt
     7741 tcgagcgtga aggcaacaac ctctattgcg aagtcccgat caacttcgct atggcggcgc
     7801 tgggtggcga aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg
     7861 aaacccagac cggtaagctt ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg
     7921 gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaga
     7981 agcagaaaca gttgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggtgagcaca
     8041 acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc
     8101 gctaacctcc ccaaaagcct gcccgtgggc aggcttgggt aaaattaggg tgcgttgaag
     8161 atatgcgagc acctgaaaag tggcggggat cactccccgc cgttgctctt actcggattc
     8221 gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc
     8281 ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat
     8341 catcgcctta tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac
     8401 tctacatgtg ttcagcatat agggggcctc gggttgatag taaaatatca ctcggggctt
     8461 ttctctatct gccgttcagc taatgcctga gacagatagc ctcaagcacc cgccgctatt
     8521 atatcgctct ctttaaccca ttctgtttta tcgattctaa tcctgaagac gcctcgccta
     8581 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tatttatcgc
     8641 aattattgac gacaagctgg attatttttg aaatattgtc ctaacaaata acgccgactg
     8701 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
     8761 gattatcagg gcttagggtc acccgttagc gctgatatga ctttccgtgt
//
LOCUS       DAJTVK010000154         8635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000154 DAJTVK010000000
VERSION     DAJTVK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.156"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="OO372_003721"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="OO372_003721"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6343107.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="OO372_003722"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="OO372_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6343108.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="OO372_003723"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="OO372_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6343109.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO372_003724"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="OO372_003724"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6343110.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO372_003725"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="OO372_003725"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6343111.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="OO372_003726"
     CDS             complement(7826..8137)
                     /locus_tag="OO372_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6343112.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO372_003727"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="OO372_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR6343113.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaat
//
LOCUS       DAJTVK010000155         8466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000155 DAJTVK010000000
VERSION     DAJTVK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.157"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..124)
                     /locus_tag="OO372_003728"
     CDS             complement(<1..124)
                     /locus_tag="OO372_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6343114.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQL"
     gene            416..937
                     /gene="fecI"
                     /locus_tag="OO372_003729"
     CDS             416..937
                     /gene="fecI"
                     /locus_tag="OO372_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR6343115.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            934..1887
                     /gene="fecR"
                     /locus_tag="OO372_003730"
     CDS             934..1887
                     /gene="fecR"
                     /locus_tag="OO372_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR6343116.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1974..4298
                     /gene="fecA"
                     /locus_tag="OO372_003731"
     CDS             1974..4298
                     /gene="fecA"
                     /locus_tag="OO372_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR6343117.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            4343..5245
                     /gene="fecB"
                     /locus_tag="OO372_003732"
     CDS             4343..5245
                     /gene="fecB"
                     /locus_tag="OO372_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR6343118.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5242..6240
                     /gene="fecC"
                     /locus_tag="OO372_003733"
     CDS             5242..6240
                     /gene="fecC"
                     /locus_tag="OO372_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR6343119.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLCLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6237..7193
                     /gene="fecD"
                     /locus_tag="OO372_003734"
     CDS             6237..7193
                     /gene="fecD"
                     /locus_tag="OO372_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR6343120.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7194..7961
                     /gene="fecE"
                     /locus_tag="OO372_003735"
     CDS             7194..7961
                     /gene="fecE"
                     /locus_tag="OO372_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR6343121.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
BASE COUNT         1855 a         2417 c         2356 g         1838 t
ORIGIN      
        1 gtagttgcca tgttttacgg caatgagagc agagatagcg ctgatgtccg gcagtgcttt
       61 tgccgttacg caccacgcct tcagtagcgg agcaggaagg acatctgatg gaaatggaag
      121 ccacgcaagc accttaaaat caccatcata cactaaatca gtaagttgac agcattaccg
      181 aaatccctat cctttagggc agtgaggatg tcaataatag cagaccacat catgagtcat
      241 tctgaccgac acatgccaga agcagaggct ccatcccatt ttatacctac cttataacac
      301 ttagaaaaac aacatgttaa aaatgtctat tggaaacaat tttatttcca attgtaatga
      361 taactattct catattaata tggctacgtg ataattaact tttgatgcac tccgcatgtc
      421 tgaccgcgcc actaccacag cttccttaac attcgagtcg ctttatggca cacatcacgg
      481 ctggttgaaa agctggctga cgcgcaaact ccagtctgct tttgatgcag atgacattgc
      541 ccaggacact tttttgcggg taatggtcag cgaaacgctc tcgacgatcc gcgatcctcg
      601 ctccttcctc tgcactatcg ccaaacgcgt gatggtggac ctgtttcgcc gaaacgcgct
      661 ggaaaaagcg tatctggaga tgctggcgct tatgccggag gggggagcgc cttcacctga
      721 ggaacgcgaa agccaactcg agaccctaca actcctcgac agcatgctgg acgggctaaa
      781 cggcaaaaca cgtgaagcgt ttctgctttc gcaactggat ggtctgacat acagcgagat
      841 tgcgcacaaa ctcggtgttt ccatcagctc cgtgaaaaaa tacgtggcga aagccgtcga
      901 gcactgcctg ctgttccgtc tggagtatgg gttatgaatc ctttgttaac cgattcccgc
      961 cgtcaggcgc tgcgttcagc ttcccactgg tatgccgtgc taagcggcga gcgcgtcagc
     1021 ccacaacagg aagcgcgctg gcaacagtgg tatgaacagg atcaggataa ccagtgggcc
     1081 tggcagcagg ttgaaaacct gcgcaaccag cttggcggtg tgcctggcga cgttgccagc
     1141 cgggcgttgc acgatacccg cctcacccgc cgtcacgtga tgaaaggatt actgctgttg
     1201 ctcggcgctg gcggaggctg gcagctctgg cagtcggaaa ccggcgaagg tctgcgggca
     1261 gattaccgca ccgccaaagg cacagtcagc cgtcagcaac tggaagatgg ctccctgctc
     1321 acgctgaata cccaaagcgc ggcggatatg cgttttgatg cgcatcagcg caccgtccgg
     1381 ctctggtacg gtgaaatcgc cattactacc gcgaaagatg ccctgcaacg ccccttccgt
     1441 gtcctgaccc gtcagggcca gctcactgct ttagggacag aatttaccgt ccgccagcag
     1501 gataatttca cgcagcttga cgtgcagcag cacgccgtgg aagtgcttct cgccagtgcc
     1561 cccgcgcaaa aacgcatcgt gaacgctggt gaaagcctgc agttcagcgc ctctgagttt
     1621 ggcgcagtga aaccgctgga tgacgagagt acaagctgga cgaaggacat cctgagcttc
     1681 agcgataaac cgctgggtga ggtgatagcc acgctaaccc gttaccgcaa cggcgtgctg
     1741 cgctgcgatc ccgccgttgc cgggctgcgc ctgagcggga cgttcccgct gaaaaatacc
     1801 gatgcgatcc tgaacgttat cgcgcaaacg cttcccgtta aaattcagtc tattacgcgg
     1861 tactggataa acatttcacc actgtaagga aaataattct tatttcgatt gtccttttta
     1921 cccttctcgt tcgactcata gctgaacaca acaaaaatga tgatggggaa ggtatgacgc
     1981 cgttacgcgt ttttcgtaaa acaacacctt tggttaacgc cattcgcctg agcctgctgc
     2041 cgctggccgg tctctcgttt tccgcttttg ctgcacaggt taatatcgca ccgggatcgc
     2101 tcgataaagc gctcaatcag tatgccgcac acagcggatt taccctctcg gttgacgcca
     2161 gcctgacgcg cggcaagcag agcaacggcc tgcacggcga ttacgacgtc gagagcggcc
     2221 tgcaacaact gctggacggc agcggactgc aggtaaaacc gctgggaaat aacagctgga
     2281 cgctggagcc cgcgcccgca ccaaaagaag atgccctgac cgtggtcggc gactggctgg
     2341 gtgatgcgcg tgaaaacgac gtatttgaac atgctggcgc gcgtgacgtg atccgccgtg
     2401 aggatttcgc caaaaccggc gcaaccacca tgcgtgaggt acttaaccgc atccctggcg
     2461 tcagcgcgcc ggaaaacaac ggcaccggca gccacgacct ggcgatgaac tttggcatcc
     2521 ggggcctgaa cccgcgcctc gccagccgct cgaccgtcct gatggacggc atccccgtcc
     2581 ccttcgcccc ttacggtcag ccgcagcttt cactggctcc cgtttcgctc ggcaacatga
     2641 atgccattga cgtggtacgc ggtggtggtg cggtgcgtta cggaccgcag agcgtgggcg
     2701 gcgtggtgaa ctttgttacc cgtgccattc cgcaggactt tggtatcgag gcgggcgtgg
     2761 aaggtcagct cagcccaacc tcttcacaaa acaacccgaa agagacgcac aacctgatgg
     2821 tgggcggcac agcggacaac ggttttggca ccgcgctgct ctactccggc acgcgcggca
     2881 gtgactggcg cgagcacagc gccacccgca tcgacgacct gatgctgaaa agcaaatatg
     2941 cgccggatga ggtgcacacc ttcaacagcc tgctgcaata ttacgacggt gaagccgaca
     3001 tgcccggtgg cctgtctcgc gcggattacg acgccgatcg ctggcaatcc acccgcccgt
     3061 atgaccgctt ctggggtcgt cgcaagctgg cgagcctggg ctaccagttc cagccagaca
     3121 gccagcataa attcaacatt caggggttct acacccaaac cctgcgcagc ggctacctgg
     3181 agcaaggcaa acgcatcacc ctctcgccgc gtaactactg ggtgcgcggt attgagccac
     3241 gctacagcca gatctttatg atcggccctt ccgcgcacga agtgggcgtg ggctatcgct
     3301 atgtgaatga atcaacgcat gaaatgcgtt actacaccgc caccagcagc gggcagttgc
     3361 cgtccggctc aagcccttac gaccgcgata cgcgttccgg caccgaggcg cacgcctggt
     3421 atctggatga caaaatcgac atcggcaact ggaccatcac gccgggtatg cgtttcgaac
     3481 atatcgagtc ataccagaac aacgccatca caggcacgca cgaagaagtg agctataacg
     3541 caccgcttcc ggcgttgaac gtgctctatc acctgactga cagctggaat ctttatgcaa
     3601 acactgaagg ctcgttcggc accgtacagt acagccagat tggcaaggct gtgcaaagcg
     3661 gcaatgtgga accggaaaaa gcgcgaacct gggaactcgg tacccgctac gacgacggcg
     3721 cgctgacggc ggaaatgggg ctgttcctga ttaactttaa caatcagtac gactccaacc
     3781 agaccaacga caccgtcact gcacgtggca aaacgcgcca taccgggctg gaaacgcagg
     3841 cacgttacga tctgggtacg ctaacgccaa cgcttgataa cgtttccatc tacgccagct
     3901 atgcgtatgt gaacgcggaa atccgcgaga aaggcgacac ctacggcaat ctggtaccat
     3961 tctccccgaa acataaaggc acgctgggcg tggactacaa gccaggaaac tggatgttca
     4021 atctgaacag cgatttccag tccagccagt ttgcggataa cgccaatacg gtgaaagaga
     4081 gcgccgacgg cagtaccggc cgcattcccg gcttcatgct ctggggcgca cgcgtggtgt
     4141 atgactttgg cccgcagatg gcagatctga acctggcatt cggtgtgaaa aacatcttcg
     4201 accaggacta cttcatccgc tcttatgacg acaacaacaa aggcatctat gcaggccagc
     4261 cgcgcacgct gtatatgcag ggagcgttga agttctgatg tgttaacgcc cggcttgccg
     4321 ggcttttagc tggaatgtga ttatgttggc atttatccgt tttctttttg caggcctgct
     4381 gctggtgatc agccacgcct ttgccgccac ggttcaggac gaacacggca cgtttacact
     4441 cgaaaaaacg ccacaacgga ttgtggtgct ggaactctcg ttcgccgatg cgctggccgc
     4501 cgtggacgtc agcccgatcg gtattgccga cgataacgat gcaaaacgca tcctgcccga
     4561 agtgcgtgcg cacctgaaac cgtggcagtc cgtcggaacg cgcgcgcagc cgagcctgga
     4621 agccattgcc gctctgaaac cagacctgat cattgccgac agcagtcgcc atgcgggggt
     4681 ttacatcgcc ttgcagcaaa tcgcgtcggt actgctgctt aagtcccgca acgaaaccta
     4741 cgctgaaaat ttgcaatctg cggctatcat cggcgaaatg gtgggtaaaa agcgagagat
     4801 gcaggcacgt ctggaacaac ataaagagag gatggcgcag tgggccagcc agcttcccaa
     4861 agggacacgc gtggcctttg gcacatcacg cgaacagcaa ttcaacctgc atactcagga
     4921 gacctggacc ggcagcgtgc tggcctctct ggggctgaac gttcccgctg cgatggcggg
     4981 tgcgtccatg ccgtccatcg gcctggagca actgctggcg gtcaatcctg cctggctgct
     5041 ggttgcccac tatcgcgaag agagcattgt taaacgctgg caacaagatc cgctctggca
     5101 gatgttaacc gccgcgcaga agcagcaggt tgcttcggtc gacagtaaca cctgggcgcg
     5161 gatgcgcggt atttttgctg cagagcgtat tgccgctgac acggtaaaaa tcttccacca
     5221 tcagccgctt accgttgtga aatgaccgcg ataaaacacc cggtgctgct gtgggggctt
     5281 cccgttgcag cacttattat tattttctgg ctgagtctgt tttgctactc ggccattcct
     5341 gtttccggag cagatgcaac ccgcgccctg ctgcctggac acacgccaac gctaccagaa
     5401 gcgctggtgc aaaaccttcg tttgccacga agcctggtcg ccgttctgat cggcgcaagc
     5461 ctggcgctcg cgggcacgct gctgcaaacc ctgacccaca acccaatggc ctctccttca
     5521 ctgctcggca ttaacagcgg cgcggcgctg gctatggcgc ttaccagcgc gctgagtccg
     5581 acgccgattg caggctattc tctgtcgttc atcgcggcat gcgggggcgg cgtgagctgg
     5641 ctgctggtca tgaccgcagg aggcggattt cgtcataccc atgacagaaa caaactgatc
     5701 ctcgcgggta tcgcgctgtc ggccttttgt atgggcctta cccgcatcac cctgctgctg
     5761 gccgaagatc atgcttacgg catcttttac tggctggctg gcggagtgtc ccacgcccgc
     5821 tggcaggatg tctggcagct cttgccggtg gtggtcactg cagtccctgt cgtgttgctg
     5881 ctggcgaatc aactgaacct gctcaacctc agcgacagca ccgcccatac gctgggagtg
     5941 aacctgacga ggctatgttt ggtcatcaat atgttagtgc tgcttctggt tggtgcgtgc
     6001 gtcagcgtgg caggtccggt ggcgtttatc ggtctgctgg tgccacatct ggcgcgcttc
     6061 tgggcaggct tcgatcagcg caacgtactg ccagtgagca tgctgctggg ggccacgctg
     6121 atgctgctgg cagatgtact cgcacgcgcg ctggccttcc ccggagatct gcccgcaggc
     6181 gcagtgctgg cgctgattgg cagcccttgc tttgtctggc ttgtgaggag gcgaggatga
     6241 aaattgcgct ggttattttc atcacccttg ccctggcggg ctgtgcgctg ttatcactcc
     6301 atatgggagt gatccccgtg ccgtggcgcg cgctgctgac cgactggcag gccggacgcg
     6361 agcattatta tgtattgatg gagtaccgac tgccgcgctt gctgctggca ctgtttgtcg
     6421 gtgcagccct cgccgtggcg ggcgtgctga tacaggggat tgtgcgcaac cctctggcat
     6481 caccggatat tctcggcgtt aaccatgccg ccagcctggc ctctgtgggg gctctacttc
     6541 ttatgccgtc actgcccgtg atggtgctgc cgctgctggc ctttgcgggc ggcatggcgg
     6601 ggttgatatt gctgaagatg ctggcaaaga cccaccagcc gatgaagctg gcactcaccg
     6661 gcgtggcgct ttctgcatgc tgggccagcc tgacggatta tctgatgctc tcgcgcccgc
     6721 aggatgtgaa caacgccctg ctgtggctga ccggcagctt atggggccgt gactggagct
     6781 ttgtgaagat tgccatcccg ctgatgattt tatttctgcc gctgagcctg agtttttgcc
     6841 gcgatctcga cctccttgca ctcggcgatg cgcgcgccac cacgctcggt gtgtcggtgc
     6901 cctatacccg attctgggct ttgttactag ctgtcgccat gacatctacc ggcgtggccg
     6961 cctgcggccc gattagcttt attggtctcg tggtgccgca tatgatgcgt agcatcaccg
     7021 gtggacgtca ccgcagactg ctgcctgttt cggccctgac aggtgcgttg ctgttggtgg
     7081 ttgccgatct gctggcgaga attattcatc ccccactgga gctcccggtt ggcgtgctga
     7141 ccgccattat cggtgcgccg tggtttgtct ggttgcttgt gagaatgcga taaatgactt
     7201 tacgaactga aaatctgacg gtcaattacg gaacagacaa ggtacttaac gacgtttcac
     7261 tctcactgcc aacggggaag atcaccgccc tgatcggtcc taacggttgc gggaaatcga
     7321 cgctgttaaa ctgtttttcg cggcttttaa tgccgcagtc tggcaccgta tttctcggcg
     7381 ataatcccat aaatatgctc tcatcgcgcc agttggcccg caggctttcg ctgctgcctc
     7441 agcaccattt aacgccagag gggatcacag tccaggagct ggtttcgtat ggtcgtaatc
     7501 cctggctgtc actctggggg cgtctctccg ctgaagacaa tgcacgagtt aatgtcgcca
     7561 tgaaccagac ccggatcaat catcttgccg ttcgtcggtt aaccgagctt tccggcggtc
     7621 agcgccagcg cgcatttctg gcaatggtcc tggcccagaa tacgcccgtt gtattacttg
     7681 atgagccaac cacctatctt gatatcaatc accaggtgga cctgatgcgg ttgatgggcg
     7741 aactccggac tcaggggaaa acggtggtcg ctgtgctgca cgaccttaat caggctagcc
     7801 ggtactgcga tcaactggtg gtaatggcaa acggacatgt tatggcgcaa ggcacaccag
     7861 aagaggtgat gaccccagga ttgctgagaa cagtattcag cgtggaagcg gaaatacacc
     7921 ccgagccggt atctggcagg ccgatgtgcc taatgaggta gattgcacag gccgtaagaa
     7981 ccaaaccacg actgaatgaa actggactgg cgccagcaag cctgttcaga ctggggctga
     8041 acttttccgg actctgaaag attaccaata ctcatcgtcc atccgcttgc tttaggctga
     8101 caggttcata atcaacgcaa accagagctg tacaggcttg ggcgcggctt tcaaaccagt
     8161 cgtgatcacg gcaatcaatt ttgaactctg cttaacggac atttctgtat aacccttacg
     8221 gcaacgaaaa acgcgaagtt aaaattttag aaacccaaaa acgtgacatg actaagttta
     8281 gatttcaggg gggggagatc aaaaaatttc gctctgtgcc agagcggaca ttcacggagc
     8341 tggttcatta ccaatgaggt tgggcttttg aggataaatc aatgatcaga cgccaacgta
     8401 aatcaaaagc aaccctggaa cggaattgct aatccagttt ctgaccatcg atttttctaa
     8461 aaagtg
//
LOCUS       DAJTVK010000156         8331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000156 DAJTVK010000000
VERSION     DAJTVK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.158"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(327..1163)
                     /locus_tag="OO372_003736"
     CDS             complement(327..1163)
                     /locus_tag="OO372_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6343122.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1163..1966)
                     /locus_tag="OO372_003737"
                     /note="aph(3'')-Ib"
     CDS             complement(1163..1966)
                     /locus_tag="OO372_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6343123.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(2027..2842)
                     /gene="sul2"
                     /locus_tag="OO372_003738"
     CDS             complement(2027..2842)
                     /gene="sul2"
                     /locus_tag="OO372_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6343124.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            4031..4180
                     /locus_tag="OO372_003739"
     CDS             4031..4180
                     /locus_tag="OO372_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343125.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(4947..5387)
                     /locus_tag="OO372_003740"
     CDS             complement(4947..5387)
                     /locus_tag="OO372_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6343126.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            5605..5994
                     /locus_tag="OO372_003741"
     CDS             5605..5994
                     /locus_tag="OO372_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343127.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6038..6226
                     /locus_tag="OO372_003742"
                     /pseudo
     CDS             6038..6226
                     /locus_tag="OO372_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(6193..7392)
                     /gene="tet(A)"
                     /locus_tag="OO372_003743"
     CDS             complement(6193..7392)
                     /gene="tet(A)"
                     /locus_tag="OO372_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6343128.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            7498..8148
                     /gene="tetR(A)"
                     /locus_tag="OO372_003744"
     CDS             7498..8148
                     /gene="tetR(A)"
                     /locus_tag="OO372_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6343129.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT         1888 a         2295 c         2300 g         1848 t
ORIGIN      
        1 gtccagggcg gccgtcttca cgttctgcct tgcgcagatg agatagatct agcgtggact
       61 caaggctctc gcgaatggct cgcgttggaa actttcattg acacttgagg ggcaccgcag
      121 ggaaattctc gtccttgcga gaaccggcta tgtcgtgctg cgcatcgagc ctgcgccctt
      181 ggcttgtctc gcccctctcc gcgtcgctac ggggcttcca ggggtcgttt gcgggaaggg
      241 gcggaatcct acgctaaggc tttggccagc gatattctcc ggtgagattg atgtgttccc
      301 aggggatagg agaagtcgct tgatatctag tatgacgtct gtcgcacctg cttgatcgcg
      361 gccgcgatag ctagatcgcg ttgctcctct tctccatccg cgttccaagc tgcggaaagg
      421 cacccataag cgtacgcctg gtcgagcagg cgacgcggat cgacgtccag cgcacgagag
      481 aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac aaaggtcgtc tctgtcagcc
      541 ggatcgtaga acatattggc ggcgccaaag cccacttcac cgaccagacc gacgggatct
      601 atcaccagcc agccgcgact ggagaacatg atgttttcat gatgcagatc gccatgtagc
      661 ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta taatcgccgc gtggacgtag
      721 tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct gaaacaaagc tgcaaagcga
      781 tcccggatcg ggagaagggc agaaggcagg ggttcctcag atgcggcata cagcttcgcc
      841 attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt gctcggcaac gatgtgagag
      901 agcattcgct ccccggcata ttcgagcaac atcagattgt tctcacgacc gagcaaccgg
      961 actgctcccc tcccattgcg ccataccaga tagtcggccc cgcgcagttc atcagcaatg
     1021 tcttctatag gtttcaatcc cttgacgatt gcaggagtcc cgtctggcaa tgaaactttc
     1081 caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt gcgaaacgtg ccaatgagca
     1141 ggaaaaacag gcggcatgaa catcaacccc aagtcagagg gtccaatcgc agatagaagg
     1201 caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt gaataggaca gcgaaggcgc
     1261 gctctgcttc atctggcgct gcccagttct cttcggcgtt agcaatcatg agtgccaaat
     1321 cggcatagcg atctgctgtt ccgagccgcc caaggtcgat cagacccgtg cattgaagag
     1381 ttttagggtc caccatgaag ttcggcatgc agggatcacc atggcaaaca accatatcgg
     1441 tgcgctcttg gtcgagccgc accggtagct ctcgttcgac acgagccaaa agatcgagct
     1501 gcggcgtact cttgtcctcg tccggtaaga agtcgggatt gacggcattg cgggacacca
     1561 catcaacggc gcgtccgaac attcgcgaca gcctgcgctc aaacggacat tgatcaaccg
     1621 ataggctgtg aacagcgcca agttgctgcc ccattgacgg ccacgctttg agcaaatccg
     1681 ctccagacag atcagccgcc ggtactcccg gaattgccgt tatcaccaag catgcaccct
     1741 cctgttcctc ctgccagttg atgacctcgg ggcaagccac acctcgacct ttgagccaaa
     1801 tgaggcggtc acgctctcca gcgagctcac cgcggcggga agcaggtgcg attttcgcga
     1861 aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct ctgacaggca
     1921 accagtcaga atgcgattca ccaaaaaaaa tattagttcg attcaatgga ggttccttca
     1981 gttttctgat gaagcgcgaa tatagagaaa tatcccgaat gtgcagttaa cgaattcttg
     2041 cggtttcttt cagcgccgcc aataccgcca gcccgtcgcg caaggggcgc ggctcgtgtg
     2101 tgcggatgaa gtcagctcca cctgcggcgg cggcaagctc tgcagcgagt gtcgcggccc
     2161 cgacatcccc cggaccacgg cctgtgagcg cgcgcagaaa ggatttgcgc gaaacagaca
     2221 gaagcaccgg caaatcgaag cgcagccgca attcatcgaa ccgcgccagc accgagagcg
     2281 aggtttcggg agcagccccc agaaaaaacc ccatgccggg atcaaggaca aggcggttgc
     2341 gtttgatacc ggcacccgtc agcgccgcga tgcgcgcgtc aaagaacgcc gcaatgtgat
     2401 ccatgatgtc gccagcgggt gcctcgcgcc gatctgcctg cccgtcttgc accgaatgca
     2461 taacgacgag tttggcagat gatttcgcca attgcggata gaacgcagcg tctggaaaac
     2521 cgcgaatatc attgagatag gccacaccac gcgacaaggc ataggcttgc gtcgcgggtt
     2581 gataactgtc gagcgagacg ggaatgccat ctgccttgag cgcgtccagc accggcgcga
     2641 tacgcgcgat ttctgtgtcg gacgaaacag gcgcggcgtc gggattgctg gatgccggac
     2701 cgaggtcgat cacatctgcc ccctcggcca tcagcttacg cgcctgcgca atggctgcgt
     2761 ctggcgccag ataccggcct ccatcggaga aactgtccga ggttatgttg acgatgccga
     2821 aaatgatgag cgatttattc atgggggctt ctataataat aataatcgag catgagtctc
     2881 atacggatgc tcgggtcgaa agggaatccc caggcgagta acctgtttgc ggtgatccgt
     2941 ttcatgatga tgtatctgta gcattttgga aataaatata tcatctctca ataagtagat
     3001 atatttatct tttttgcttc tttggtgctg caagtcacca atacgaggga aacctgttac
     3061 ggagcggttt aaccgtaatc aacagtgact ggtgtcatgt tcggaagatt aattgatagt
     3121 aatctacaaa ggatcttctt gagatccttt tcaatgaatc gtaatctctt gctctgtaaa
     3181 cgaaaaaacc gcctggggag gcggtttgtt cgaaggttaa gtcagttggg gaactgctta
     3241 acccggtaac agggttcacg agaccgccat taccaaaact gttctttcag tgtagccgca
     3301 gtcaggcctc tacttcaaga cctcttaatc tatcttctcg tgcactcctg ttactaatgg
     3361 ctgctgccag tggcgttttg tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg
     3421 acaaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
     3481 ctgccttcac ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat aacagcggaa
     3541 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcta ccagggggaa
     3601 acgcctggga tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt
     3661 tgcgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgtg gccttatctc
     3721 ttctttgcta agtgtcttcc tggtatcttc caggaaattt ccgtccccgt ccgtaagccg
     3781 gtaccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat
     3841 atctcctgta tcgcatattc tgctgacgcg ccggtgctgc cttttttctc ctgccacatg
     3901 aagcacttca ctgattttcg caatcgtggc aacatagtaa gccagtatac actccgctag
     3961 cgctacatga ctggttcagg gctgcgcccc gaaacccgct aacaacctct cagctactgg
     4021 atatcacccc atgccatctg aatatgatga acaactggat tcgactgata cgcagcttcc
     4081 cggcgaatat gacctttttg atttaatggc gcaggttact gaagagaacc tgcatgcccc
     4141 tgtggatttt gggtcgcctg tcggccggga agcgttatag ctttttcgct tgcccgcgta
     4201 cgtggtaata ctccggtatt accttttatt tctttatttt catgtaatta cagtggattt
     4261 ttaatacccg ggtattactc gtgaaaacga aatattcctt ttttttcttt taattcatgc
     4321 agttgcgatg aaaatcgcgg ctgcgttagg tgtatgacca ttttgaggtt gaaaaataat
     4381 caaccatggc gggtgattta cgcaggttaa ggggagatcg tgacaaaaaa agcgttcctc
     4441 actgtatcgt tcatgcctgc atgaacgggt tattcttcct gactacagaa ttacatcgct
     4501 ccttcagttc atacatcact tttacatcac ttatacatca cttttacatc acttatacat
     4561 cacttttaca tcattccttc agctcatacg tcactttcat cctctgtgcc atttgtacat
     4621 cacccccttc ctgaacgtgc tgacagcggg ttttccggct gcgccggcaa cgcttcacgg
     4681 ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaacgc cggaaacagg
     4741 acttaccccg gctgaatggt gtgaatttcc gcgctatgca cttgtgcgca tactcatgca
     4801 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     4861 taccggtttc ccgtcagaaa cgccctgagg gcctctcagg cggtgctgat aacagatgtt
     4921 atggtaaatc agagtcacct gcactgttac tctgaaccgg agcgaaacag ccgcgaccag
     4981 aatccccgct ttttttcctg tgccagcgcg tcagccagac ggctaatttc ggcctgtagt
     5041 tgttccctct cctcatttcg caggcggttt tctgcttccc gcgccatctt gtcttcaagc
     5101 attagggtaa cagtctgctg cagagcgtcc agtttctgct gaatgacgtc tagctgagca
     5161 ttttccgcct ggctaacgac agatttggag tgtccctcct tgtgcctttt tggtgtctca
     5221 gaagtgcgca gctcgccata ggcccgaatc agttcactgg tctcgaactc ccggcgatca
     5281 ttttttcctg tgcgatagct gacaaggcct ttagccatgt cacgatataa ttgcgagcgc
     5341 gactttcctg ttaatttcag tgtctcttga actgtgtgcc atgccataaa agtgacagtg
     5401 tctcctgtgt gtaccgtatt gtaacagcag aacatattac tgttttaaca caaaaggtct
     5461 gaggctgccc gtgaggcgtg aaaaatacac aaaaaacctg ttgacggtgt ggttttgacg
     5521 ggcgtagtat ttcgaatact gtatataaac acattgttat aaatacagtt tttgctgttt
     5581 ttaaaagtca aagaggataa ttttatgagt acagagttaa atagcggtac tgtcggtggt
     5641 tttggtcaca tgggaccaaa tgacccctac aatgatggtc atagcggctc tggaagttcg
     5701 ggaggtagtt ttaacggtgg tggtaatggt ggccatgata atagtagtgg ttcatcttat
     5761 actgcatcct ttggtctcat cactggccga cacccgataa ctgtcccagg tacgaagaat
     5821 attaattctg tcgatctttc tgctgtcatt aataacatta tacatcatga aggtgcacgc
     5881 aataacatgt acctagatac tgcgaacaat gtaagagtgg gagtgacggg cactggctgg
     5941 caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg ctaagcgata
     6001 gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg cggccaggac
     6061 ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc aaacccttct
     6121 ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag agcagggaaa
     6181 ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaaagcc cgcgacgcag
     6241 cgccggcagg cagagcaagt agagggcagc gcctgcaatc catgcccacc cgttccacgt
     6301 tgttatagaa gccgcataga tcgccgtgaa gaggaggggt ccgacgatcg aggtcaggct
     6361 ggtgagcgcc gccagtgagc cttgcagctg cccctgacgt tcctcatcca cctgcctgga
     6421 caacattgct tgcagcgccg gcattccgat gccacccgaa gcaagcagga ccatgatcgg
     6481 gaacgccatc catccccgtg tcgcgaaggc aagcaggatg tagcctgtgc cgtcggcaat
     6541 cattccgagc atgagtgccc gcctttcgcc gagccgggcg gctacagggc cggtgatcat
     6601 tgcctgggcg agtgaatgca gaatgccaaa tgcggcaagc gaaatgccga tcgtggtcgc
     6661 gtcccagtga aagcgatcct cgccgaaaat gacccaaagc gcggccggca cctgtccgac
     6721 aagttgcatg atgaagaaga ccgccatcag ggcggcgacg acggtcatgc cccgggccca
     6781 ccggaacgaa gcgagcgggt tgagagcctc ccggcgtaac ggccggcgtt cgcctttgtg
     6841 cgactccggc aaaaggaaac agcccgtcag gaaattgagg ccgttcaagg ctgccgcggc
     6901 gaagaacgga gcgtgggggg agaaaccgcc catcagccca ccgagcacag gtcccgcgac
     6961 catcccgaac ccgaaacagg cgctcatgaa gccgaagtgc cgcgcgcgct catcgccatc
     7021 agtgatatcg gcaatataag cgccggctac cgccccagtc gccccggtga tgccggccac
     7081 gatccgcccg atatagagaa cccaaaggaa aggcgccgtc gccatgatgg cgtagtcgac
     7141 agcagcgccg gccagcgaga cgagcaagac cggccgccgc ccgaaacgat ccgacagcgc
     7201 gcccagcaca ggtgcgcagg caaattgcat caacgcatac agcgccagca gaatgccata
     7261 gtgggcggtg acgtcgttcg agtgaaccag atcgcgcagg aggcccggca gcaccggcat
     7321 aatcaggccg atgccgacag cgtcgagcgc gacagtgctc agaattacga tcaggggtct
     7381 gttgggtttc acgtctggcc tccggaccag cctccgctgg tccgattgaa cgcgcggatt
     7441 ctttatcact gataagttgg tggacatatt atgtttatca gtgataaagt gtcaagcatg
     7501 acaaagttgc agccgaatac agtgatccgt gccgccctgg acctgttgaa cgaggtcggc
     7561 gtagacggtc tgacgacacg caaactggcg gaacggttgg gggttcagca gccggcgctt
     7621 tactggcact tcaggaacaa gcgggcgctg ctcgacgcac tggccgaagc catgctggcg
     7681 gagaatcata cgcattcggt gccgagagcc gacgacgact ggcgctcatt tctgatcggg
     7741 aatgcccgca gcttcaggca ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc
     7801 ggcacgcgac cgggcgcacc gcagatggaa acggccgacg cgcagcttcg cttcctctgc
     7861 gaggcgggtt tttcggccgg ggacgccgtc aatgcgctga tgacaatcag ctacttcact
     7921 gttggggccg tgcttgagga gcaggccggc gacagcgatg ccggcgagcg cggcggcacc
     7981 gttgaacagg ctccgctctc gccgctgttg cgggccgcga tagacgcctt cgacgaagcc
     8041 ggtccggacg cagcgttcga gcagggactc gcggtgattg tcgatggatt ggcgaaaagg
     8101 aggctcgttg tcaggaacgt tgaaggaccg agaaagggtg acgattgatc aggaccgctg
     8161 ccggagcgca acccactcac tacagcagag ccatgtagac aacatcccct ccccctttcc
     8221 accgcgtcag acgcccgtag cagcccgcta cgggcttttt catgccctgc cctagcgtcc
     8281 aagcctcacg gccgcgctcg gcctctctgg cggccttctg gcgctcctgc t
//
LOCUS       DAJTVK010000157         8327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000157 DAJTVK010000000
VERSION     DAJTVK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.159"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            267..1925
                     /locus_tag="OO372_003745"
     CDS             267..1925
                     /locus_tag="OO372_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6343130.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="OO372_003746"
     CDS             complement(1922..2878)
                     /locus_tag="OO372_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6343131.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="OO372_003747"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="OO372_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6343132.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="OO372_003748"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="OO372_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6343133.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO372_003749"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="OO372_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6343134.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6789..8027
                     /locus_tag="OO372_003750"
     CDS             6789..8027
                     /locus_tag="OO372_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6343135.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         2057 a         1960 c         2233 g         2077 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc
     6661 attcatcgct atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg
     6721 gtttgccctt gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa
     6781 cgcgaaacat gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg
     6841 gatcaccaac cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt
     6901 ccaccagcgg cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat
     6961 caccggaacg cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa
     7021 aagcggcaca gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc
     7081 gtgctgtaca ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg
     7141 aatcgtatgc aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac
     7201 acataatcag ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg
     7261 catgttttga ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat
     7321 gcaaacaggc gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg
     7381 atatggttgt cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca
     7441 aggagcttgg ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg
     7501 ccctgattcg cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg
     7561 tccaggaatg ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga
     7621 aggtagcaat attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg
     7681 agcagaacag gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag
     7741 agtacggaag aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc
     7801 ggcacacgtt tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc
     7861 taaccgcgca ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg
     7921 caaaacgtga cgctcttaat ctatgggttg agcgtctcga gcttctccag aacaatgatg
     7981 aaaaaatcgt tgttatgacc ccgcgaattt actcccaaaa ttcttgacaa attacggctg
     8041 tttttcttgc gaaaaatggc cctctaaaat aaatcgtcga atatcggcaa atatcgacga
     8101 tttatatgtg tcaactatcg aaaaaaattg aaatgtcttc ttctgagttc ttattctctg
     8161 tttcttgatt attccaatac ctgatggttt atctatttgt ttttaatgag gtaattcagg
     8221 tgtcatgcca aatggcgaca ttttttgttg cgtcgcttaa aaactcgtga cacgatttta
     8281 aacactaacg agcttctacg aaccttagcg aacaaagcct ttagcat
//
LOCUS       DAJTVK010000158         8272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000158 DAJTVK010000000
VERSION     DAJTVK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.160"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            52..936
                     /locus_tag="OO372_003751"
     CDS             52..936
                     /locus_tag="OO372_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6343136.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO372_003752"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO372_003752"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR6343137.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO372_003753"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO372_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6343138.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO372_003754"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO372_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6343139.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO372_003755"
     CDS             5160..6443
                     /locus_tag="OO372_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR6343140.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO372_003756"
     CDS             6590..8065
                     /locus_tag="OO372_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6343141.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1914 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc gg
//
LOCUS       DAJTVK010000159         8215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000159 DAJTVK010000000
VERSION     DAJTVK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.161"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(216..1106)
                     /gene="dgcZ"
                     /locus_tag="OO372_003757"
     CDS             complement(216..1106)
                     /gene="dgcZ"
                     /locus_tag="OO372_003757"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR6343142.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1361..1753)
                     /gene="ydeI"
                     /locus_tag="OO372_003758"
     CDS             complement(1361..1753)
                     /gene="ydeI"
                     /locus_tag="OO372_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR6343143.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2029..2547
                     /locus_tag="OO372_003759"
     CDS             2029..2547
                     /locus_tag="OO372_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6343144.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2592..4637)
                     /gene="dcp"
                     /locus_tag="OO372_003760"
     CDS             complement(2592..4637)
                     /gene="dcp"
                     /locus_tag="OO372_003760"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6343145.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4774..5520
                     /gene="ydfG"
                     /locus_tag="OO372_003761"
     CDS             4774..5520
                     /gene="ydfG"
                     /locus_tag="OO372_003761"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6343146.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5609..6295
                     /gene="ydfH"
                     /locus_tag="OO372_003762"
     CDS             5609..6295
                     /gene="ydfH"
                     /locus_tag="OO372_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6343147.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6472..6675
                     /gene="ydfZ"
                     /locus_tag="OO372_003763"
     CDS             6472..6675
                     /gene="ydfZ"
                     /locus_tag="OO372_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6343148.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6711..8171)
                     /locus_tag="OO372_003764"
     CDS             complement(6711..8171)
                     /locus_tag="OO372_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6343149.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2172 a         2055 c         1870 g         2118 t
ORIGIN      
        1 acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag gggcctgatt
       61 tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca tggatagatt
      121 tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc gttgacacag
      181 tagcatcagt tttctcaatg aatgttaaac ggagcttaaa ctcggttaat cacattttgt
      241 tcgtcaataa acatgcagcg atttcttccg gtttgcttac cctcatacat tgcccggtct
      301 gctcttccaa tgaccacatc cagaggctct tcaggaaatg cgcgactcac acctgctgtc
      361 acggtaatgt tgatatgccc ttcagaatgt gtgatggcat ggttatcgac taactggcaa
      421 attctgacac ctgcacgaca tgcttcttca tcattagccg ctttaacaat aatgataaat
      481 tcttcgcccc cgtagcgata aaccgtttcg taatcacgcg tccaactggc taagtaagtt
      541 gccagggtgc gtaatactac atcgccgatt aaatgcccgt aggtatcatt aaccaattta
      601 aatcggtcaa tatccaacaa cattaaataa agattcagag gatcagtgtt gcgtaactga
      661 tgatcaaagg attcatcaag aacccgacgc cccggcaatc ccgtcaaaac atccatattg
      721 ctacggatcg tcagcaaata aattttgtaa tcggttaatg ccgcagtaaa agaaagcaac
      781 ccctcctgaa aggcgtcgaa atgcgcgtcc tgccagtgat tttcaacaat agccagcatt
      841 aattcccgac cacagttatg catatgttga tgggcagaat ccattagccg aacgtaaggt
      901 aattcatcgt tatcgagtgg ccccagatga tcaatccacc gaccaaactg gcacagtcca
      961 taagaatggt tatccgttat ttctggctta ctggcatctc tcgcgaccac gctgtgaaac
     1021 atactcacca gccactggta gtgggcatcg atagccttat tgagatttaa caagatggca
     1081 tcaatttccg ttgtcttctt gatcattgcc actccttttt cacagttcct tgtgcgcgct
     1141 attctaacga gagaaaagca aaattacgtc aatattttca tagaaatccg aagttatgag
     1201 tcatctctga gataacattg tgatttaaaa caaaatcagc ggataaaaaa gtgtttaatt
     1261 ctgtaaatca cctctgcatt atcgtaaata aaaggatgac aaatagcata acccaatacc
     1321 ctaatggccc agtagttcag gccatcaggc taatttattt ttatttctgc aaatgagtga
     1381 cccgaacgac ggccggcgcg cttttcttat ccagactgcc gctaatgttg atcatctggt
     1441 ccggctgaac ttctcgtcca tcaaagacgg ccgcaggaat aacaacatta atttcaccgc
     1501 tcttatcgcg aaaaacgtaa cggtcctctc ctttgtgaga aatcaaatta ccgcgtagtg
     1561 aaaccgaagc gccatcgtgc atgttttttg cgaaatcaac ggtcattttt tttgcatcat
     1621 cggttccgcg atagccatct tctattgcat gaggcggcgg tggcgctgca tcctgtttta
     1681 aaccgccctg gtcatctgcc aacgcataag gcatgacaag aaaacttgct aatacaatgg
     1741 actgaaattt catactaact ccttaattgc gtttggtttg acttattaag tctggttgct
     1801 atttttataa ttgccaaata agaatactgc caattgttat aaggcattta aaatcagccc
     1861 actagctgtc aaatattcag agaatttgac tcactaaagt taagaagatt gaagagtctt
     1921 aaacatgttt tcagaataat cggatttata tgtttgaaaa ttattatatt ggacgagcat
     1981 acagaaaaag caaatcacct ttacatataa tagcgtggac aaaaaacagt gaacattaat
     2041 aaagataaaa ttgtacaact cgcagatacc gacactattg aaaacctgac atccgcgttg
     2101 agtcaaagac ttatcgcgga tcaattacgc ttaactaccg ccgaatcatg caccggcggt
     2161 aagttggcta gcgccctgtg tgcagctgaa gatacaccca aattttacgg tgcaggcttt
     2221 gttactttca ccgatcaggc aaagatgaaa atcctcagcg taagccagca gtctcttgaa
     2281 cgatattctg cggtgagtga gaaagtggca gcagaaatgg caacaggtgc catagagcgt
     2341 gcggatgctg atgtcagtat tgccattacc ggctacggcg gaccggaggg cggtgaagat
     2401 ggtacgccag cgggtaccgt ctggttcgcg tggcatatta aaggccagaa ctacactgcg
     2461 gttatgcatt ttgctggcga ctgcgaaacg gtattagctt tagcggtgag gtttgccctc
     2521 gcccagctgc tgcaattact gctataacca ggctggcctg gcgatatccc caggccagcc
     2581 attggtggtg tttatatgtt caagccacga tgttgcagca tcggcataat ctgaggagcc
     2641 ttaccgcgcc attgtcgatg caggcgttcc agatcttcgc tgttacctct ggaaaggatc
     2701 gcctcgcgaa aacgctgccc attttcacgc gttaatccgc cctgctcaac aaaccactgg
     2761 taaccatcat cggccaacat ttgcgtccac agataagcgt aataacctgc agcatatccg
     2821 ccaccaaaaa tatgggcgaa ataactgctg cgatagcgtg gcggtatagc aggaagatcc
     2881 atattttccg ccaccagcgc ccgcaattca aaatcatcga catcctgcat tgcttcgttt
     2941 tcttccaggc aatgccagcg catatcgaga agtgcggcgc taagcagttc gctcatctca
     3001 taccctttgt tgaacaggct ggcattacgc attttctgtt gcagttcgtc aggcattgct
     3061 gccccgctct gataatgccg ggcgtagcga gcgaatacct gcggatgcgt tgcccagtgt
     3121 tcgttgattt gcgacggaaa ttcgacaaaa tcacgcggcg tgttggtgcc ggaaagcgtg
     3181 gcataacgct ggcgggcaaa aaggccgtgc agcgtatgac caaattcatg gaataaggtt
     3241 attacatcat cccagagtaa caacgcaggc tcaccggcag cgggtttctg ataattgcag
     3301 acgttataaa ttaccggatg cgtttcatta agcgttgatt gctcaacaaa attgcccatc
     3361 catgcaccgc cgctttttga atcacgggcg aagaaatcac cgtaaaataa cgccagcccc
     3421 acgccattat gatcaaaaat ttcccacacc cgaacgtcag gatggtagac aggaatatca
     3481 aaacgttcga caaacttaat accgaagagc tgattcgcgg tccagaatac accttcattc
     3541 aacaccgtgt ttaattcaaa atatggcttg agctgcgctt catcaagatc aaatttctcc
     3601 cgccgtacct gttccgcata aaatgcccag tcccacggct gcgcgctaaa cccgccctgc
     3661 tgcttatcga taaccgcctg tatggaggct aattcatcgc tcgcacgttg acgcgccgct
     3721 ggaacaattt cccgcataaa gttgagtgct gcttctggcg tttttgccat ctgatcggcg
     3781 attttccatg cggcataatg aggaaaacca agcagtttcg cctgctgtgc gcgaatctct
     3841 accagacgtt gaatgatagc gcgggtatca ttgccatcat ttttttccgc tcgcgtccag
     3901 cccgcaataa acagtttttc acgcgtcgca cgatcgcgca tttcggcaag cgccggttgt
     3961 tgggtggtat tcagcagcgg aatcagccat ttgttatcca gacctttctc gcgagccgcc
     4021 tctgccgcca gcgcaatctc ttgctcactc attcctgcca gctgcgcgat atcgttcaca
     4081 accagaccgc cggatttatt tgctgccagt aatcgctggt taaactggct ggtcagggtc
     4141 gcagcttctg tattcagtac ttttaatttt gctttatcag cttgcgcaag tttggctccg
     4201 gcaaggacaa aacgttgatg aatcacctcc accaggcgga tggattcact atcaagcccc
     4261 agggattcac ggcgctgcca gacagcatct acccgcgcga ataattcacc gttcagatag
     4321 atatcattag ccagttccgc cagttcagcg gaaaactgtt catcaagacg ctgtaattca
     4381 tcattggtat gcgccgcagt catcgcaaaa aagacgctgg taacgcgggt aagtaattct
     4441 ccgctttgtt ccagtgccag aatagtattg ttgaaatcag gcgtttgcgg gttaagcgcg
     4501 atggcagcaa tttctgcccg cttttgctgc attccctcat cgaatgccgg gcgatagtga
     4561 tgattggcaa tttgatcaaa atggggagcc agatacggta gtgtgctttg cacaaggaaa
     4621 ggattcattg ttgtcatttt cttctcctga acgcgagatg ttccatagcg taggcttact
     4681 gataacggag tgcaatcttg caaaccagta ttacccgctc ttaagcatca cgtgctatgt
     4741 tattgacaca caaaagcgtt gaggaacagt gagatgatcg ttttagtaac tggagcaacg
     4801 gcaggttttg gtgaatgcat tactcgtcgt tttattcaac aagggcataa agttatcgcc
     4861 actggccgtc gccaggagcg gttgcaggag ttaaaagacg aactgggaga taatctgtat
     4921 atcgcccaac tggacgttcg caaccgcgcc gctattgaag agatgctggc atcgcttcct
     4981 gccgagtggt gcaatattga tatcctggta aataatgccg gcctggcgtt gggcatggag
     5041 cctgcgcata aagccagcgt tgaagactgg gaaacgatga ttgataccaa caacaaaggc
     5101 ctggtatata tgacgcgcgc cgtcttaccg ggtatggttg aacgtaatca tggtcatatt
     5161 attaacattg gctcaacggc aggtagctgg ccgtatgccg gtggtaacgt ttacggtgcg
     5221 acgaaagcgt ttgttcgtca gtttagcctg aatctgcgta cggacctgca tggtacggcg
     5281 gtgcgcgtca ccgacatcga accgggtctg gtgggtggca ccgagttttc caatgtccgc
     5341 tttaaaggcg atgacggtaa agcggaaaaa acctatcaaa ataccgttgc attgacgcca
     5401 gaagatgtca gcgaagccgt ctggtgggtg tcaacgctgc ctgctcacgt caatatcaat
     5461 accctggaaa tgatgccggt tacccaaagc tatgccggac tgaatgtcca ccgtcagtaa
     5521 tttttatacc cggcgtaact gccgggttat tgcttgtcac aaaaaagtgg tagactcatg
     5581 cagttaactc actcacaagc aagaacgaat gaccgtcgaa acgcaactta atcccacaca
     5641 gcctgtcaat cagcaaattt atcgtattct tcgtcgcgac attgtccatt gcctgattgc
     5701 accaggcaca ccgttgtcgg aaaaagaagt ttctgttcgt ttcaatgtgt cacgccagcc
     5761 ggttcgtgaa gcctttatta aactggcgga aaacggcctg attcaaattc gtccgcagcg
     5821 tggcagctac gtcaacaaaa tttccatggc ccaggtgcgc aatggcagtt ttatccgtca
     5881 ggccattgag tgcgcggtgg cgcgtcgggc ggcgagcatg attaccgaaa gccagtgcta
     5941 ccaactggaa caaaatcttc accagcaacg cattgccatt gagcgcaagc aactggatga
     6001 tttttttgaa cttgatgaca acttccatca actcctgacg cagattgccg actgtcaact
     6061 ggcgtgggat accattgaga acctgaaagc gaccgttgat cgcgtgcgct atatgagttt
     6121 cgaccacgtt tctccaccag aaatgctgtt acgccagcat cttgatattt tctctgccct
     6181 gcaaaaacgt gatggcgatg cggtagaacg tgcaatgacg caacatttgc aggaaatcag
     6241 cgaatccgtg cgccagatcc gccaggaaaa tagcgactgg tttagcgaag agtaattcat
     6301 ttcctctcat cccatccggg gtgagagtct tttcccccgc cttatggctc atgcatgtat
     6361 caaaaaagat gtgagcttga tcaaaaacaa aaaatatttc actcgacagg agtatttata
     6421 ttgcgcccgt tacgtgggct tcgactgtaa atcagaaagg agaaaatacc tatgacgacc
     6481 tacgatcgta accgtaacgc aatcaccact ggcagccgtg ttatggttag cggcaccggt
     6541 cacactggca agatcctgtc gattgatact gaaggtctga ccgctgagca aatccgccgc
     6601 ggaaaaaccg tagttgttga aggttgtgaa gagaaactgg cacctctgga cctgattcgt
     6661 ctcggcatga actaagcgtg tgaatgccgc cgatggcggc attgcttttt ttacttcacg
     6721 gaatatttcg ccacggtcgc tttcgcgcca tgcgctaata aagacaagta cgcttccgtc
     6781 acttttgcag taaacaaact attgtctggc aaatcatcac caaagatcgc cttaatcgcc
     6841 agcaatgact ggacgcgcgc tttcccttca gcactacttt gtacagcctt ctgaataaca
     6901 ggtaacagtg ggtcactgat ttcaatcgga tttccctgtt catcaacacc accgacataa
     6961 cgcatccaac ccgcgacgcc cagtgccagc agatcgaact tgctgtcatg cgccagatgc
     7021 cagcgaacag aatccaacat ccgctgtggc aatttctggc tgccatccat cgcaatctgc
     7081 caggttcgat gacgtaacgc cgggttgcta tagcgttcaa ttaatcggtt agcgtaatct
     7141 tgcaaatcaa cgccctgcac tttcagcgtc ggcgcttgtt cctgcaacat caagccatac
     7201 gccgcataac gataatgttc atcttccatg cagtcattaa tgtgctgata tccagcaaga
     7261 taacccaaat acgccaggaa tgaatgactg ccgttgagca tgcgcaactt catctcttca
     7321 taaggcagca catcactaac cagttcggct cccgcttttt cccattctgg acgtccggca
     7381 acaaagttat cttctattac ccactggcgg aaaggttcac aggcaacgcc cgcaggatcg
     7441 cccacaccgg taagttgttc gattttcgcc agcgtatcct ctgtcactgc gggcacaata
     7501 cggtccacca ttgttgatgg gaaagtcacg ttatcttcga tccattgtgc cagttttaca
     7561 tcaacagctt tcgcgtatga agtgacaacg tcacgcataa catgaccgtt ttctggcatg
     7621 ttgtcacatg acatgacggt aaatgcggga agtcctgccg ctttacggcg ggccagcgcc
     7681 tcaacaatca cccctgttgc tgttttcggc tggtggggat tttgcacgtc ggcagcgacc
     7741 atcgggtgat cgagcattaa ctgtccggtc gccggagagt ggaaataccc tttttcggtg
     7801 attgtcagag agacaatcgc gatttgcggt tcacacatcg cagccaacac ggtttctaag
     7861 ccatccatct gtacgtgcaa ggctttctta acgacgccaa cgacgcgagc cgtccacgca
     7921 tcggccgaca tttccgcaac ggtataaaga ttatcttgct gttgtaaatc ggcaatttgc
     7981 tgttcgccgc cgattaagtt gacctcataa tatccccagt cactgaaatg ttctgtagca
     8041 agaatatcgg catacacgcc ctgatgcgca cggtgaaatg caccaaagcc taaatgaata
     8101 attcttgggg ccaggttatt acgatcataa acagggagtg tcgcttttgc tgataacaaa
     8161 ttatttccca taacaattcc ttaaatataa atatggcaaa ctatatgttt tgtta
//
LOCUS       DAJTVK010000160         8182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000160 DAJTVK010000000
VERSION     DAJTVK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.162"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="OO372_003765"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="OO372_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR6343150.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /locus_tag="OO372_003766"
     CDS             complement(2975..3697)
                     /locus_tag="OO372_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6343151.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="OO372_003767"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="OO372_003767"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6343152.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="OO372_003768"
     CDS             6679..>8182
                     /locus_tag="OO372_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCR6343153.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2030 c         2321 g         1852 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAJTVK010000161         8157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000161 DAJTVK010000000
VERSION     DAJTVK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.163"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..449)
                     /locus_tag="OO372_003769"
     CDS             complement(<1..449)
                     /locus_tag="OO372_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6343154.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO372_003770"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="OO372_003770"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6343155.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="OO372_003771"
     CDS             complement(1788..2507)
                     /locus_tag="OO372_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6343156.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="OO372_003772"
     CDS             complement(2691..3017)
                     /locus_tag="OO372_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6343157.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO372_003773"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="OO372_003773"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6343158.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="OO372_003774"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="OO372_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6343159.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="OO372_003775"
     CDS             4977..5252
                     /locus_tag="OO372_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6343160.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="OO372_003776"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="OO372_003776"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6343161.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="OO372_003777"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="OO372_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6343162.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="OO372_003778"
     CDS             complement(7903..>8157)
                     /locus_tag="OO372_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6343163.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2160 c         1987 g         2088 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaattc
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DAJTVK010000162         8123 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000162 DAJTVK010000000
VERSION     DAJTVK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8123
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.164"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            163..317
                     /locus_tag="OO372_003779"
                     /pseudo
     CDS             163..317
                     /locus_tag="OO372_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="OO372_003780"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="OO372_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCR6343164.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="OO372_003781"
     CDS             858..2264
                     /locus_tag="OO372_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCR6343165.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /locus_tag="OO372_003782"
     CDS             2509..3654
                     /locus_tag="OO372_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6343166.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="OO372_003783"
     CDS             3672..4685
                     /locus_tag="OO372_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6343167.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="OO372_003784"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="OO372_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="OO372_003785"
     CDS             5576..6370
                     /locus_tag="OO372_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6343168.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..7816
                     /gene="patD"
                     /locus_tag="OO372_003786"
     CDS             6392..7816
                     /gene="patD"
                     /locus_tag="OO372_003786"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6343169.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8026..>8123
                     /locus_tag="OO372_003787"
     CDS             8026..>8123
                     /locus_tag="OO372_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCR6343170.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         1919 a         1908 c         2222 g         2074 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt
     7621 atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac
     7681 ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc
     7741 tggttacggc aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt
     7801 catggttaaa cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag
     7861 gttctgagtt taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat
     7921 taggctaaat gtttgatggc gatttttcat cctgtcacag ttgattagca taaggcttta
     7981 gagtatcgtt attaaactaa taatttataa gtagaagtat taacaatgaa agttcaattg
     8041 tacaaattca cggaagataa aaataaaacc cttaccttca gatggacgaa aaaacacttt
     8101 gagttttgta tggataataa aat
//
LOCUS       DAJTVK010000163         8114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000163 DAJTVK010000000
VERSION     DAJTVK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.165"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..613
                     /locus_tag="OO372_003788"
                     /pseudo
     CDS             1..613
                     /locus_tag="OO372_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1227..1603)
                     /gene="rnpB"
                     /locus_tag="OO372_003789"
     ncRNA           complement(1227..1603)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO372_003789"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1636..2781)
                     /gene="garK"
                     /locus_tag="OO372_003790"
     CDS             complement(1636..2781)
                     /gene="garK"
                     /locus_tag="OO372_003790"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6343171.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2878..3768)
                     /gene="garR"
                     /locus_tag="OO372_003791"
     CDS             complement(2878..3768)
                     /gene="garR"
                     /locus_tag="OO372_003791"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6343172.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3798..4568)
                     /gene="garL"
                     /locus_tag="OO372_003792"
     CDS             complement(3798..4568)
                     /gene="garL"
                     /locus_tag="OO372_003792"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR6343173.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4584..5918)
                     /gene="garP"
                     /locus_tag="OO372_003793"
     CDS             complement(4584..5918)
                     /gene="garP"
                     /locus_tag="OO372_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6343174.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            6293..7864
                     /gene="garD"
                     /locus_tag="OO372_003794"
     CDS             6293..7864
                     /gene="garD"
                     /locus_tag="OO372_003794"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6343175.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            8013..>8114
                     /gene="prlF"
                     /locus_tag="OO372_003795"
     CDS             8013..>8114
                     /gene="prlF"
                     /locus_tag="OO372_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCR6343176.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
BASE COUNT         2122 a         2130 c         1912 g         1950 t
ORIGIN      
        1 atgttccagg ttaattttgt tagttcaaca caaaacgcca caatctcctg ggattattta
       61 ctaaaaataa cgcctgtttt aataagcatt agcgatatgt attctgaaga aaaaatcaag
      121 tttgtcgaaa gttgtttaaa tgagcttgga gacattaccg aagaacaatt aaaaattatg
      181 agatttgcaa ttataaaatc tataccaagg gcaactctta cagataaatt agaaaatgaa
      241 ttaacaaaag aaatatataa aagctcatcg aaaatcaaca attgcttgaa cagaattaaa
      301 ttaccagaga tgaaagaatt ctcatcagaa aaaaatatat gattacatcg atataatcat
      361 tgaagattat gaaaatatca aagaaaatgc ttatctggtc gatccaaaaa ttaattacac
      421 tatagattta aacatagaag actctagctc agaggaattc ctttttgata atagcctcga
      481 gaaagacgaa aattcttcgg acaattgctt tgaggtcgaa aaatataacg catatgaagc
      541 atataactca gagaatctat attttaccag agaggaatat acgtatgatt acgacctttt
      601 aaatgcaata tagacataat aacttcttac aaaatctata attaataccg tagatattca
      661 ttcatcttac cctgtgcacc aaaagcaaaa actcactgac gcgggtttga aactgatggc
      721 aaccgcaaaa atgcctgatg cgctacgctt atcaggccta cgccatctct acaatatatt
      781 gaatttgcgt gcttttgtag gcaggataag gcgttcacgc agcatccggc atgaataaag
      841 cgcactcaac aatctgaaac ccgccggaac ggtttattac gtacatcagg tgaaactgac
      901 cgataagccg ctttcttttg ggtatagtgt cgtggacagt cattcatctt tctgcccctc
      961 caaaagcaaa aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag
     1021 ccgctttctt ttgggtatag cgtcgtggac agtcattcat ctttctgccc ctccaaaagc
     1081 aaaaacccgc cgaagcggtt ttttacgtaa accaggtgaa actgaccgat aagccgcttt
     1141 cttttgggta tagcgtcgtg gacagtcatt catctttccg ctcctccaaa agcaaaaacc
     1201 cgccgaagcg ggtttttacg taaatcaggt gaaactgacc gataagccgg gttctgtcgt
     1261 ggacagtcat tcatctaggc cagcaatcgc tcactggctc aagcagccta cccgggttca
     1321 gtacgggccg taccttatga acccctattt ggccttgctc cgggtggagt ttaccgtgcc
     1381 acggactgtt accagccgcg cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc
     1441 cgcttgcgcg ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt ttccccccca
     1501 ggcgttacct ggcaccctgc cctatggagc ccggactttc ctcccctccg cccgtctccc
     1561 ccgaagagga cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc agtatagagg
     1621 gtttgcgcgc ccttgtcacc ccgcgttgcg cattccaatc gccagtgtcg cggcgatatt
     1681 acgtgaagca cggcagatat tgtcataagc cccgcggaat gcttcgtcca acgtacctat
     1741 gctggtcaat acgctgaaga ccgcatcgat gccatgctga tgtacaacgc caacatcatc
     1801 ggtcaggcta cccgcaatgc caatcaccgg tttatggtac ttcttcgcca cgtttgcaac
     1861 gccaatcggt accttgccgt gaatactctg gctgtcaata cgcccttcac cggtgatcac
     1921 cagcgtacaa tcgtgaatat gttcttccag attcagcgcc gtagtgacga tttcaatacc
     1981 acttttcagt tccgcaccaa gaaacgccat cagcgccgcg cccataccgc ccgcagctcc
     2041 tgcgccgggg acatctttca catcaacatg cagcgctttt ttaatgacat cggcatagtg
     2101 agagaggtta ttgtccagct caacaatcat cgcttcactg gctccctttt gtgggccaaa
     2161 gatgcgcgat gcgccgttat cgcccaccag cggattggtg acatcacaag cgacgcgaat
     2221 gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca atatcattca gagtattaag
     2281 actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg cccccagcgc
     2341 ctgtaccatg cctgcgccgc catcatttgt agcgctgccg ccaatgccga taataatgtt
     2401 tgttgcactg ctctccagcg cctgcaggat taactcgcct gtgccgcgtg aagtggttac
     2461 gagtggatcg cgtttttccg caggtaccag ctccagccca ctggccgccg ccatttcaat
     2521 aaacgcggtt ttgccatcgc cggagatccc ccaactggca ttcactttct cgcccagcgg
     2581 ccctgtaacc caggcgtgac gttcagcccc ctgggtggct gcaatcatcg cttccaccgt
     2641 tccttcacca ccgtcggcaa ccggaacaga aacatactgt gcatcaggaa aaatttcccg
     2701 aaatcctttt tctatcgcct gcgcaacctc gctggcagat aaactttctt tataagagtc
     2761 tggggcgatt acgattttca tacctatgcc tgttaccaca tgacgccgga gggcgtttct
     2821 cttattcggc ctagattcca ggcccggatt gcaatacacc atccgggcac gacgtcatta
     2881 acgagtaact tcgactttcg ccagtttttc gtagtagcac gccagggcgc tatgatccgc
     2941 cgttcctaaa ccatctgcac gcagtgcctg catcatctcc ataaccgcag ctgtgagcag
     3001 cagttgtgcg ccgacgccgt gagaagtatc cagcgcattc gccagatcct taatatgcag
     3061 atcaatgcgg aagcccggct tgaagttgcg gtccatcacc atcggcgctt tggcatccag
     3121 cacggtactg cccgccagcc caccgcgaat tgcctgataa accaggtccg ggttaacgcc
     3181 cgctttggtt gccagcgtta acgcttctga catcgcagca atattcagcg ccacaatgac
     3241 ctgatttgcc agtttggtga cgttacctgc accgatttcc ccggtatgca ccacggaacc
     3301 cgccatcgct ttcatcaaat catagtattt gtcgaaaata gccttgtcgc cgcccaccat
     3361 cactgacagc gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag catccagcat
     3421 atcaatgcct ttcgctttca gcgcttcgct gatttcacgg cttgccagcg gtgcgataga
     3481 actcatatcg atcaataccg tacctggctt cgcgccttca ataatgccat tctcacccag
     3541 cgccacctct ttcacatgag gggagtttgg cagcatggtt atgatgacgt cgcactgttc
     3601 agcgatcgct ttagccgtag acgctgtttc tgcacctgca gcaatcacgt cagcaatagc
     3661 ttctgggtta cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt ttttactcat
     3721 tggtttaccc ataatcccca ggccaataaa accaactttc atagtcatat caaccatctc
     3781 tcttgttgcg gtggtggtta ttttttaaag gtatcagcca gtttctgagt ggcagagcgg
     3841 aagacgccaa gatcgctgcc gacagccaca aacgtcgcgc cccattccag ataacgacgc
     3901 gcatcggctt cgaccggcgc gagaataccg ctgggtttgc cgtgcgcgct ggcacggtta
     3961 aaaatatgct gaattgcttt ttgtacatcc ggatgcgagg cattgccgag atggcccaat
     4021 gccgcagcca gatcgctggg gccgacgaag ataccgtcta caccttcggt agcggcaata
     4081 gcatcgacgt tatctacgcc ctgctgactt tctatctgga ccagaatagt aatgttcttg
     4141 ttcgactgag cgaaataatc cgccacggtg ccaaacatat tggtgcggtg agaaacggag
     4201 acgccgcgaa tgccttccgg cgggtaacga gttgatgcca ccgcctgctc tgcttcctct
     4261 tttgtttcta caaaaggaat caggaagtta tagaaaccga tatccagaag acgcttaata
     4321 attaccggct cgttggtcgg cactcgcact actggcgcgc tggcgctgcc tttcagggcc
     4381 attaactgcg gaataaacgt ggaaatatcg tttggcgcat gttcgccatc cagcaccagc
     4441 cagtcaaacc cagccaaacc aagaacttca gtgctaatcg ggttagagag tgctgaccag
     4501 caaccaattt gtacctgttt cgcagccagt gcggctttga atttattcgg gaaaacatcg
     4561 ttattcatcg cttatacctt tgcttatttc tgcaattcca tacgtttaat gtcgccaact
     4621 acgaagaggt agcagaccat cgccatcagc gctgaacatc ccacgaaaac cagtgctgca
     4681 ttgaaggaat gcagttcact taccaggtag ccaatcacca gtggagtgac aatggaggca
     4741 acattgccaa agacgttaaa gacgccgcca cagaggccaa caatctcttt cggcgcggtg
     4801 tcagaaatca ccggccagcc cagagcacca aatcctttgc caaaaaaagc cagcgccatc
     4861 agcatgacca ccagcgtggt gttgttggtg tagttacata agatgatggt ggaaaccagc
     4921 aacattccca gcacaatcgg tagcttacgt gccagggtca gggataaacc gcgtttgatc
     4981 agataatccg agaagacacc tcccagcacg ccgcccgcaa aaccacacag tgccggaatc
     5041 gaggcgacca gacccacttt cagaatcgac atgccttttt cctgcaccag ataaatcggg
     5101 aaccaggtga ggaagaacca ggtgatggtg ttgataaaat attgtccgaa aaatacgccc
     5161 aacatcatgc ggttagagag caattgcttg atgtaatgca gtttgggacc gcttgctgcc
     5221 gcgctgcccg gctttttgtg gtccatatcg accaccgcgc cattttcaga gataaacttc
     5281 agctcttccg cagacatacg tgggtgatct gtcgggttat gaatcaactt gatccacagc
     5341 gccgtcagca caaaaccaat cccccccata acggtgaaga cgtgctccca gccccaagcg
     5401 aaagtcagcc agccaagcag cggcgaaaag agcgccagcg agaaatattg cgccgagtta
     5461 aagatggcgg aggcagtacc acgttctttc gtcgggaacc aggcggcgac aatacgggcg
     5521 ttcgccggga atgatggcgc ttccgagaac cccagcataa agcgcataaa gaacatggag
     5581 atccctgccc aggccagcgg gaacatatca acaaagcctt gcaggaaggt gaacagcgac
     5641 cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa acttatcaag caaccagccg
     5701 ccggggattt gcatcagcaa gtaggcccag ccaaaagcag agaagatgta acccatcgaa
     5761 accgcactta actgcaactc ttttgccact tcggtaccag caatagacag cgttgcacga
     5821 tcggcgtagt taacggcggt aacaataaaa ataatcagta atattaaata gctggtatgc
     5881 acgcctttct tttttacgtc aacggtgtcc agaatcattt tatttacctc gggtacttat
     5941 gctgattttt attattatgg ggaaggtgtt atttatgagt tttatttatg ccgttacgac
     6001 aatgaactcg ggaattagta taagcagcgg gagaataata atcattgtgc aaatgctaat
     6061 ttaatcagta ctatttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc
     6121 acagatttgc gctttacctt accgggcggc actgcaatcc ctgaaatgat tgacattgat
     6181 cacatttctg cgtttaaact cctgacattc ttatttcacc caatgaagtc atttattttt
     6241 aaatgagacc agatcctcat tttaataacc cctggctgga gaatattgca caatggccaa
     6301 catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa
     6361 tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc cggatgggct
     6421 ggaattaatt gaacatactc cccaggggca taaagtcgca ttgctggaca ttccagctaa
     6481 tggtgaaatt attcgttatg gcgaagtgat tggttacgcc gtgcgcgcaa tcccacgcgg
     6541 aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac acacgctgcc
     6601 actggcaacc aaagtcccgg cccccttacc gccgctggaa ggatacacct ttgagggcta
     6661 tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca
     6721 ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa
     6781 atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggctgtg gcgtggcgat
     6841 taacgcaccg gcggcagttg tgcctattcg taccatccac aatatttcgc tgaaccctaa
     6901 ctttggcggc gaagtaatgg tgattggtct gggttgtgaa aagttgcagc ctgagcgcct
     6961 gctggtcggc acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt
     7021 gcaggatgaa aagcatgtcg gctttcagtc catggtcgag gatattttgc aggtcgccga
     7081 acgccatcta caaaaactga atcaacggca gcgagaaacc tgcccggctt cagaactggt
     7141 cgttggtgtg cagtgcggtg gcagcgatgc gttttctggt gtaacggcaa acccggcggt
     7201 tggctatgcg tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt cagaagtaac
     7261 ggaagtgcgt gacgcgatcc atctgctgac accacgcgca gtgaacgaag aggtcggcaa
     7321 acggctgctg gaggagatgg agtggtacga taactatctc aatatgggaa aaaccgaccg
     7381 cagcgccaac ccttcgccgg gcaacaagaa aggcggtctg gcaaatgtgg tggagaaggc
     7441 actcggctcc attgctaaat cgggtaaaag tgcaattgtt gaagtgctgt cgcccggtca
     7501 acgcccgact aaacgcggat taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg
     7561 cacgcaacag gtggcttcgg gtatcaccgt acaagtgttt acgaccggcc gtggtacgcc
     7621 gtacggcctg atggcggtac ccgtaattaa aatggcaacc cgcaccgagc tggcgaaccg
     7681 ctggtttgat ttaatggata ttaacgcggg cactatcgct accggcgaag agagcattga
     7741 agaggtgggc tggaagttgt tccactttat tctcgacgtc gccagcggga agaagaaaac
     7801 cttctcggat caatgggggc tgcataacca gctggcggtg tttaacccgg caccggtgac
     7861 ctgatttctt ttggaacgtc cctcgcaaaa catggcctta gtgccatgtt tttattgttt
     7921 aaagccccca cgtccattaa taatgcattt gcattacctt agttcaagct tataatttga
     7981 gcagaaaaca ggctgtaaaa ggacaatgaa tcatgcccgc taatgctcgc tctcacgctg
     8041 tactgaccac tgaatcaaag gtcacgatac gcggacaaac aactatcccc gcgccagtgc
     8101 gtgaggcctt aaaa
//
LOCUS       DAJTVK010000164         8104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000164 DAJTVK010000000
VERSION     DAJTVK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.166"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(101..796)
                     /locus_tag="OO372_003796"
     CDS             complement(101..796)
                     /locus_tag="OO372_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6343177.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="OO372_003797"
     CDS             complement(789..2216)
                     /locus_tag="OO372_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6343178.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="OO372_003798"
     CDS             complement(2227..2946)
                     /locus_tag="OO372_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6343179.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEALPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO372_003799"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="OO372_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCR6343180.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="OO372_003800"
     CDS             4554..5879
                     /locus_tag="OO372_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCR6343181.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSLEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /locus_tag="OO372_003801"
     CDS             5988..6224
                     /locus_tag="OO372_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6343182.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="OO372_003802"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="OO372_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCR6343183.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="OO372_003803"
     CDS             complement(6989..7858)
                     /locus_tag="OO372_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6343184.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2192 a         1982 c         1733 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa gcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc ctcgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAJTVK010000165         7947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000165 DAJTVK010000000
VERSION     DAJTVK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.167"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..359
                     /locus_tag="OO372_003804"
     CDS             <1..359
                     /locus_tag="OO372_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCR6343185.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="OO372_003805"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="OO372_003805"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6343186.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3127..3960
                     /gene="ppsR"
                     /locus_tag="OO372_003806"
     CDS             3127..3960
                     /gene="ppsR"
                     /locus_tag="OO372_003806"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6343187.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4117..5163
                     /gene="aroH"
                     /locus_tag="OO372_003807"
     CDS             4117..5163
                     /gene="aroH"
                     /locus_tag="OO372_003807"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6343188.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5295..5486
                     /gene="hemP"
                     /locus_tag="OO372_003808"
     CDS             5295..5486
                     /gene="hemP"
                     /locus_tag="OO372_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6343189.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5490..6926)
                     /locus_tag="OO372_003809"
     CDS             complement(5490..6926)
                     /locus_tag="OO372_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6343190.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="OO372_003810"
     CDS             complement(6989..7702)
                     /gene="ydiV"
                     /locus_tag="OO372_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6343191.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2031 a         2086 c         1888 g         1942 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt actttttaag actgatccca gcgttgcgca
     2941 aatctgctct cttcgacaat ttccacataa accatcggta taaacgaaac cataaaaatg
     3001 aaatgctgtt ttcataaaaa ataaaattga aggttcattt tataaaccag acataacgtc
     3061 ctacgcttct gtgcgttttt aatttatgct ttcatagaat tatgtctgca tcacgggaag
     3121 aacaaaatgg ataatgctgt tgatcgccac gttttttata tttctgatgg tacggcaata
     3181 actgcggagg tattaggaca cgcagtaatg tcacaatttc ccgtcactat cagcagcatt
     3241 acgctgccgt ttgtcgaaaa tgagagccgt gcacgggcag tgaaggatca gattgacgca
     3301 atttatcacc agacaggtgt gcgcccgctg gtcttctact ccatcgtgtt gccggagatt
     3361 cgcgccatca tcttgcaaag tgaaggcttt tgccaggata tcgttcaggc gctggttgcc
     3421 ccgctacaac aagagatgaa actggatcca acgccgattg ctcatcgtac ccatggcctt
     3481 aaccctaata atctcaataa atatgatgcg cgcattgcgg cgattgatta caccctcgcc
     3541 cacgatgacg gcatttcgtt gcgcaatctg gatcaggctc aggtgatcct gctcggtgtt
     3601 tctcgctgtg gtaaaacccc caccagtctt tatctggcaa tgcaatttgg tatccgcgcg
     3661 gcaaactacc cctttattgc cgacgatatg gataatctgg tgctacccgc gtcgctcaaa
     3721 ccgcttcagc ataaattgtt cggcctgact atcgacccgg aacgtctggc ggcgattcgc
     3781 gaggaacgtc gggagaacag tcgctatgcc tcgcttcgtc agtgcaggat ggaagtcgcg
     3841 gaagtggaag cgttgtaccg taaaaatcag atcccgtgga ttaacagtac caattattcg
     3901 gtagaagaga ttgccaccaa gatcctcgat atcatgggcc ttagtcgccg aatgtactag
     3961 agaactagtg cattagctta tttttttgtt atgatgctaa ccacccggcg aggtgtgaca
     4021 cacctcgcac ttgaaatcag cagcgattgg tttatcgtga tgcgcatcac ttcccggcag
     4081 tcctgccgta gaagcaacaa atttctgaga cttgtaatga acagaactga cgaactccgt
     4141 actgcgcgta ttgagagcct ggtaacgccc gccgaactcg cgctacggta tcccgtaacg
     4201 cctggcgccg ccacccatgt caccgactcc cgccgcagaa ttgaaaaaat actcaatggt
     4261 gaagataagc gactgttggt cattattggc ccctgctcga tccacgatct caccgctgca
     4321 atggagtacg ccccccgtct gcagtcgctg cgcaaccagt accagtcacg gctggaaatc
     4381 gtaatgcgca cctattttga aaaaccacga actgttgtcg gctggaaagg actaatctcc
     4441 gatccagatt taaacggcag ctatcgggta aatcacggtc tggagctggc gcgcaaatta
     4501 cttttacagg taaatgagct gggcgtccca accgcgactg agttcctcga tatggtgacc
     4561 ggtcagttta ttgctgattt aatcagttgg ggcgcgattg gcgcacgtac taccgaaagt
     4621 cagatccacc gtgaaatggc ttcggcactc tcctgtccgg taggttttaa aaatggtacc
     4681 gatggcaata cgcggattgc tgtggatgct atccgcgcag cccgcgccag ccatatgttc
     4741 ctctcgccag acaaaaatgg tcagatgacc atctatcaga ccagcggcaa cccgtatggc
     4801 cacattatta tgcgtggcgg caaaaaaccg aattatcatg ccgatgatat cgccgcagcc
     4861 tgcgatacgc tgcacgagtt tgatttacct gaacatctgg tggtggattt cagccacggt
     4921 aactgccaga agcagcaccg tcgccagtta gaagtttgtg aggatatttg tcagcaaatc
     4981 cgcaatggct ctacggcgat tgctggaatt atggcggaaa gtttcctgct cgaaggaacg
     5041 caaaaaatcg tcggcagtca gccgctcact tacggtcaat ccattaccga cccgtgtctg
     5101 ggctgggaag ataccgaacg cctggtcgaa aaactcgcct ctgcggtaga tacccgcttc
     5161 tgaatgcgtg cccattcctg acggaatggg catttctgcg caacttgttg tcttctcaac
     5221 aaattactgc ttgctctggc ctgccataat attgataata agaatcattg ttatatcaat
     5281 tattattaat ctttatgcgt tatacggata gcagaaaact cacgcctgaa acggacgcca
     5341 atcacaaggc cgcttccccg cagcccatcc ggcgaattac cagccagaca ctgttaggtc
     5401 cggatggcaa actgattatc gatcatgacg ggcaagaata tctgctccgt aaaactcagg
     5461 ctggcaagct gctgttaact aagtagcctt taactcgagc agctgacttc cagccgttta
     5521 ccccagtcag gtggacgact gacatagtca tcatctctat cgctgaaagg atttcgcaac
     5581 gccccatgca ggcggtgcaa ttccgtcata tcaccctttt ctgccgcttc aatcgcccgt
     5641 tgcgccaacc agttgcgcaa taccagagcg gggttaacgc tttgcatcaa ctgctgacgc
     5701 tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat aacgggcaaa ccagccatca
     5761 aatgccgcac gatcaataaa ctcatcacgt agcggtgacg ccgtgctgtg ctgctcggtc
     5821 agactcagca tgcggaatgt gcgggtataa tcgctgcgct ctcgcgccat cagactgaat
     5881 aattcattca gtagcgcgtt atcctctttt tgctccgtga tgaagcccag tttctgccgc
     5941 atccgttgtc cataatgcgt caacaaaacc tgctgatagc tgtccagggc ctcattcagg
     6001 gcatctacgg caacaaatgg tgacaatgtc tgcgccagac gctgtaaatt ccacaacgcg
     6061 acagcaggtt gattatcaaa actataacgc ccttgatgat ccgagtgatt acaaataaaa
     6121 ccgggttcgt aatcatcaag aaaaccaaac ggcccgtaat caagcgtcag tcccagcagc
     6181 gacatgttgt cggtattcat caccccatgt gcaaagccga ccgtctgcca ttgggcaatt
     6241 aacgacgcgg tacgtgcgac aacatcgcta aaccagagac ggtatttgtc ctcatcatct
     6301 tcaagatgtg accagtaatg acgaatagca aagtcagcta actgacgaac cttttccgac
     6361 tcgcggcggt agtaaaaatg ttcgaaatga ccaaagcgca gatgacttgg tgccacacgc
     6421 atcagcatcg cgcctggctc cgccgtttcc cgatacactg gcgaatcgct ggtgacgata
     6481 cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg cctcactggc gagactttct
     6541 cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc gcgaataagg cgtcaggcca
     6601 gcacctttca gatgccagtc cattgtagtg ccatcagcaa gcagttgttc gccgagtaaa
     6661 atgccgcgcc catcacccag ttggcccgcc cagacgccga actgatgacc actgtaaacc
     6721 tgcgccagtg gtgacatacc aggcagtagg gcttcgccgc cccagacacc tgcgccattt
     6781 ttaaacagcg acgatggaat actcagcgtg ttagccagtt cggtattatg ccaaatcagc
     6841 cgggcattat ttaaaggcgt aggggaaagt gctgtatagg tttctggcaa ttcatcgcgc
     6901 cagcgggtaa caaaagacag ggtcatagat cctcctgttt gatagtgtag acggttactc
     6961 tcgtcttaaa caccagcaaa cagaagggtt atcgctgaac caacgtcgtt atctgattta
     7021 tgggaacggc aggccacagg catccctgga gagcatggaa atcgaagggg gtaatctgtg
     7081 ccaatatttc tgccgtatca atgcctcccg caatgatgca attgcaacaa ggggaaattt
     7141 gcgcctggat cgcacgaata aacggttcga aggagcggtg cgtgatttgt tgctgaataa
     7201 aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa aaccgctttc attgtgctat
     7261 tgcctgctcc caaattacct aacacaaggg gatacacttg cgataaggat aaaagaccgc
     7321 gattatcttt tccttcattg agatgcgggt aattctcatt aatcaacagt tcaataaacg
     7381 gatattttaa tagttcgcct gcgtaattat ccctctctaa taataatgta gccacttgtg
     7441 gcgttaaatt aagccaggca aagagtttat gttgaataaa aaaatgttgg catgatttta
     7501 gtaattccaa ctgctctgaa aataattgcc aatgttgctc ctcggtaagt tgcgctataa
     7561 cccgactcgt cgggatacgc accgtgccat cttcactgga gaaatgggta attaactcaa
     7621 cgccgacaag gacttgctgg ttatctctta tcggcaggaa atagcaatca gaatgataaa
     7681 gattctccaa aaaaatcttc atggtaaccg tccctctcga aggatgtttt tcagtatccg
     7741 gctaaaaggg atgaggccat aagacataac aattacagaa ggagtaactt tcatttgttc
     7801 catgttaacc actttttcag gggtcctttt tttagattat cctgattata aacgaataat
     7861 ccatttacgg aatttttgtc tgcaaaatac tactgtattc aggagtaaat ggacggcgat
     7921 gtatcatcgc cgtccatttt tcgacat
//
LOCUS       DAJTVK010000166         7944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000166 DAJTVK010000000
VERSION     DAJTVK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.168"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            111..443
                     /locus_tag="OO372_003811"
     CDS             111..443
                     /locus_tag="OO372_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6343192.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="OO372_003812"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="OO372_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6343193.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="OO372_003813"
     CDS             complement(2281..2598)
                     /locus_tag="OO372_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343194.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="OO372_003814"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="OO372_003814"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6343195.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="OO372_003815"
     CDS             complement(3985..4758)
                     /locus_tag="OO372_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6343196.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OO372_003816"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="OO372_003816"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6343197.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="OO372_003817"
     CDS             complement(5952..7307)
                     /locus_tag="OO372_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6343198.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OO372_003818"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="OO372_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6343199.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OO372_003819"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="OO372_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6343200.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DAJTVK010000167         7777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000167 DAJTVK010000000
VERSION     DAJTVK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.169"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1780)
                     /locus_tag="OO372_003820"
     CDS             complement(<1..1780)
                     /locus_tag="OO372_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6343201.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO372_003821"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="OO372_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6343202.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO372_003822"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="OO372_003822"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6343203.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO372_003823"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="OO372_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6343204.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO372_003824"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="OO372_003824"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6343205.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="OO372_003825"
     CDS             complement(5968..7005)
                     /locus_tag="OO372_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6343206.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="OO372_003826"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="OO372_003826"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6343207.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1872 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DAJTVK010000168         7715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000168 DAJTVK010000000
VERSION     DAJTVK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.170"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..643
                     /locus_tag="OO372_003827"
     CDS             <1..643
                     /locus_tag="OO372_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6343208.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="OO372_003828"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="OO372_003828"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR6343209.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="OO372_003829"
     CDS             2474..2986
                     /locus_tag="OO372_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6343210.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="OO372_003830"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="OO372_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6343211.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="OO372_003831"
     CDS             5579..5782
                     /locus_tag="OO372_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6343212.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="OO372_003832"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="OO372_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6343213.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="OO372_003833"
     CDS             complement(6827..>7715)
                     /locus_tag="OO372_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6343214.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1784 a         2101 c         2021 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAJTVK010000169         7707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000169 DAJTVK010000000
VERSION     DAJTVK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.171"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..304)
                     /locus_tag="OO372_003834"
     CDS             complement(<1..304)
                     /locus_tag="OO372_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343215.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="OO372_003835"
     CDS             complement(1125..1337)
                     /locus_tag="OO372_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HCR6343216.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1413..2030)
                     /locus_tag="OO372_003836"
     CDS             complement(1413..2030)
                     /locus_tag="OO372_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR6343217.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2297..3796
                     /gene="ansP"
                     /locus_tag="OO372_003837"
     CDS             2297..3796
                     /gene="ansP"
                     /locus_tag="OO372_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR6343218.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3911..4972)
                     /locus_tag="OO372_003838"
     CDS             complement(3911..4972)
                     /locus_tag="OO372_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR6343219.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5214..7316
                     /locus_tag="OO372_003839"
     CDS             5214..7316
                     /locus_tag="OO372_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6343220.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7352..>7707)
                     /gene="mcbR"
                     /locus_tag="OO372_003840"
     CDS             complement(7352..>7707)
                     /gene="mcbR"
                     /locus_tag="OO372_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6343221.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
BASE COUNT         1964 a         1801 c         1855 g         2087 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttatc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 ataataaata acataatcct ctggtatccc actcaaataa tttcattcct ttttaatact
     1441 tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt cgtggcatta
     1501 tcctgaaacc attcatgccc agacccccat gtgcgcatag tgcaaagata acagtccacc
     1561 agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt cagccagata
     1621 taaagcgatt tacgatattc aataacattc ttctttaact gttcaggtgc gtcaggagcc
     1681 caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac cagccagacc
     1741 aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag gtccgggcga
     1801 cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc attttccagc
     1861 gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg tgaagttccc
     1921 tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag tgttagcatc
     1981 agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat atgcccctca
     2041 caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa gtatttgtca
     2101 ttttttcgtc agttgtatga atccccttca ggattgtaag gggatgtttt gcatcgtata
     2161 tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata atggtgataa
     2221 ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt tttcattaaa
     2281 gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac acgccgcgaa
     2341 acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca atcgccaggt
     2401 acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg caggagcccg
     2461 actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct tgttttcgtt
     2521 ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg gcagttttgt
     2581 ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct ggatgtactt
     2641 catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt atatgcatta
     2701 ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt
     2761 tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct ggtttgcgct
     2821 tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg
     2881 tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg gcggcttctt
     2941 cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg cttttgcctc
     3001 cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca tggtgcctaa
     3061 agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg tggtgttgct
     3121 ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt
     3181 ctctaaactg ggtgtgccat atatcggcag cattatgaac attgtggtgc tgaccgctgc
     3241 cctctccagc ctgaactcag gtctgtactg caccgggcgt attctgcgct cgatggcgat
     3301 gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg
     3361 gattctggcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc
     3421 gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt catgggcgtt
     3481 tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag cagcggatgt
     3541 cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt ttttactgag
     3601 tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg cggcgctgcc
     3661 gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat
     3721 tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc
     3781 tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt tagattactg
     3841 acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc
     3901 gccatatcta ttacagcgca atacgaatca catcgtctgg ctgggtagct tcctgctgtt
     3961 tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac agcgccaggc
     4021 tgtttggatg agtcggcgta tcgaacgttt tcactacttt atagcttttc gcgtcaatca
     4081 cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct gggttaaaca
     4141 gcacagccag tgattccggt gctgcaacct tcgccagaat attgccatta cgggtatcga
     4201 ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgccta gcggtatcaa
     4261 ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta cggctgagga
     4321 ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg gtgtaaagac
     4381 gtttgccttt gctatcaagc gccagaccgg tactcatttt accggtgttc tggatggcgg
     4441 ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta ccaataccac
     4501 tgatgtaaac ggtgttcgtg gcatcgtcag ctaccagctc acgcggttgc agcgggcgca
     4561 cctcttccgt acgcttacga tcatccagca ccagacggcc tttcacctcg cccgttttgg
     4621 catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacagtgtc tgagtcgtgt
     4681 tattgatggt gacaccaaac ggcttgagat cgttatggat cgcctgcgtc acttccagag
     4741 tgaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt tgcgaagtgg
     4801 cgagccacag cgcgttttct tgctggctat aagccatttc gtaggcacct ttacctaccg
     4861 ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca agcaatgaac
     4921 ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc atgacgactc
     4981 cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta atcatcgtta
     5041 cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag catttcttgc
     5101 cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt ttccatgaat
     5161 ttacaatttt attaccataa cggcgttttc ccattcttaa cctggctttt ttcatgaaga
     5221 ttttttccgt ccgacagacc gttttgcccg cactacttgc cctttccccc gttgtttttg
     5281 ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca gaactggata
     5341 ccccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca ccgcgcatta
     5401 acttgtccga atcactgacc gacgtgcctg gtttgcaggt acaaaaccgg cagaactatg
     5461 cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac ggtattcgcg
     5521 gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat
     5581 ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc
     5641 tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga caacagccac
     5701 caaccattga agccagtagt tactacggca gttttggcag ctggcgctat gggctgaaag
     5761 caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc gtctcaacca
     5821 cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat ttagccaatg
     5881 ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc aatagtgtgg
     5941 atatcaaagc agatgacccg ggtgggctaa ccaaagcaga atggaaggct aatccacaac
     6001 aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact caggctgggt
     6061 tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg tatgccggag
     6121 agcgagaaac gacccagtac cagtcaatac ccatggcacc acaacttaac ccgtcacatg
     6181 cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc tggacacacc
     6241 gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa aacatgagtg
     6301 aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag tacgggcaaa
     6361 aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc tatttacaga
     6421 cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt
     6481 ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc ggtgatgcca
     6541 gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat gcctggaata
     6601 tctatctggc agccgggcga ggttttgaaa cgccgacgat taatgagctg tcttatcgtg
     6661 ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat acaattgaga
     6721 tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt cagaccgaca
     6781 ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac aaaaatgccg
     6841 gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca ggagattttc
     6901 gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac
     6961 aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag
     7021 gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg ggcgatatta
     7081 tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg
     7141 ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc gataatttat
     7201 tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga tattacgaac
     7261 cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt gagtaattac
     7321 cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg tataaaatag
     7381 caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg tcttttgctt
     7441 ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc gctggattaa
     7501 tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc tcaatcatct
     7561 cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat
     7621 taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg agcaattcct
     7681 ggagttccgt caggtcttgc ggggtga
//
LOCUS       DAJTVK010000170         7623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000170 DAJTVK010000000
VERSION     DAJTVK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.172"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..96
                     /locus_tag="OO372_003841"
     CDS             <1..96
                     /locus_tag="OO372_003841"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6343222.1"
                     /translation="PAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(152..487)
                     /locus_tag="OO372_003842"
     CDS             complement(152..487)
                     /locus_tag="OO372_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6343223.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="OO372_003843"
     CDS             complement(491..835)
                     /locus_tag="OO372_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6343224.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /locus_tag="OO372_003844"
     CDS             complement(837..1010)
                     /locus_tag="OO372_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343225.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /locus_tag="OO372_003845"
     CDS             1111..1296
                     /locus_tag="OO372_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343226.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="OO372_003846"
     CDS             1257..1376
                     /locus_tag="OO372_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6343227.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO372_003847"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="OO372_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6343228.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO372_003848"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="OO372_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6343229.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO372_003849"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="OO372_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6343230.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="OO372_003850"
     CDS             4176..4544
                     /locus_tag="OO372_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCR6343231.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /locus_tag="OO372_003851"
     CDS             complement(4652..5053)
                     /locus_tag="OO372_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR6343232.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="OO372_003852"
     CDS             5295..5588
                     /locus_tag="OO372_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6343233.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="OO372_003853"
     CDS             5660..6319
                     /locus_tag="OO372_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6343234.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="OO372_003854"
     CDS             6411..6857
                     /locus_tag="OO372_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6343235.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OO372_003855"
     CDS             complement(7064..>7623)
                     /gene="hlyE"
                     /locus_tag="OO372_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR6343236.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1957 a         1714 c         1772 g         2180 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 cct
//
LOCUS       DAJTVK010000171         7491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000171 DAJTVK010000000
VERSION     DAJTVK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.173"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="OO372_003856"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="OO372_003856"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR6343237.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="OO372_003857"
     tRNA            647..722
                     /locus_tag="OO372_003857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO372_003858"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="OO372_003858"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR6343238.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="OO372_003859"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="OO372_003859"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6343239.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO372_003860"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="OO372_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR6343240.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="OO372_003861"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="OO372_003861"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6343241.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="OO372_003862"
     CDS             complement(5917..6249)
                     /locus_tag="OO372_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR6343242.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="OO372_003863"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="OO372_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6343243.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1825 a         2029 c         1868 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAJTVK010000172         7428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000172 DAJTVK010000000
VERSION     DAJTVK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.174"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..98)
                     /locus_tag="OO372_003864"
     CDS             complement(<1..98)
                     /locus_tag="OO372_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HCR6343244.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAE"
     gene            complement(123..1223)
                     /locus_tag="OO372_003865"
     CDS             complement(123..1223)
                     /locus_tag="OO372_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6343245.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="OO372_003866"
     CDS             complement(1611..2357)
                     /gene="cutC"
                     /locus_tag="OO372_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6343246.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(2371..2937)
                     /locus_tag="OO372_003867"
     CDS             complement(2371..2937)
                     /locus_tag="OO372_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6343247.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            3153..4889
                     /gene="argS"
                     /locus_tag="OO372_003868"
     CDS             3153..4889
                     /gene="argS"
                     /locus_tag="OO372_003868"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6343248.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            5066..5428
                     /locus_tag="OO372_003869"
                     /pseudo
     CDS             5066..5428
                     /locus_tag="OO372_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(5432..6502)
                     /locus_tag="OO372_003870"
                     /pseudo
     CDS             complement(5432..6502)
                     /locus_tag="OO372_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="OO372_003871"
     CDS             complement(6495..>7428)
                     /gene="flhB"
                     /locus_tag="OO372_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCR6343249.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1920 a         1946 c         1776 g         1786 t
ORIGIN      
        1 tcttctgccc atgcggggag cggtgctgcc gatgtcacca cctgcgctcc agcggcaata
       61 ccgctgtgtt taataaattc acgtcttgtt aatgtcataa cttcctccct gatcaacgag
      121 gatcactgtt tctcggtaat atctttggcg ttgtactgga aataccgcgt taaaatgtcc
      181 agttcgtttt cgctcatgct ggttcgtgcc cccattcctt tggcaatgga cggccacgca
      241 ttgacggtgt aatggtcggc ggcaataggg gcatgacaac cagcgcaata ggtatcgtca
      301 agtttttcag cgtattgcca tagcggttta cggtccgcta atccgggatc ggtaagcgca
      361 ccctgtaaag acgcctgacg ccattgattg ccgtattcgt cagcctgcca ttccccgttt
      421 accgtgagcg ccttgatacc ttcttcactt aatgtggcta gcgccagccg ttgacctgcc
      481 gccaggtaga gcgtgttttc actgccctgc atttgataac cctgcaacag aacgatcggc
      541 tgtttgccac tggcatcaac gacggtgaga tcggtaccag gattcacggt agccagctcg
      601 cctatgcgag aagttttgaa aggataaata tgtgcgccat tagtcactga agtagcggcc
      661 tgactttcca gctcatgcgc cgcgttgtca tccattttta tttctggcgg aaaatgggca
      721 atgcctttat gacaatcgat acaggtttcg ccgccctttt gtgctttgtt atgcattttc
      781 tgcgcagatt cactttgcga ggcaatatcc atggcatcaa aagaatggca actacggcac
      841 gttgcagagt cagttgcttt taattctttc catactgttt cggccatttc ctggcgatga
      901 gtttcgaact tatcgtcact gtctattttg ccgctaacaa attcatgata aatatcttta
      961 gatgctttta atttagcaaa taaataatcc atccctgact ttggaatatg gcaatctgca
     1021 cattccgcac gtatcccttt ctggttcgaa aagtggacag ttccctgata ttcctcaaaa
     1081 gatttactca tcgagtggca ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct
     1141 ttttgcgcca gcaataaccc gccgccacca accacaaccg ctatcagcaa aaacaataac
     1201 ccaatgcgtt ttttccctcg catagattcc tctaaataaa ttccagaaca ccggaatcat
     1261 tgtataatta aatacacaac gaattaaccg cttgcataac tcggcacaac actgcctgaa
     1321 ataatcgata aagaatatga tttattacaa tgtaatcatt aattgctaag gaataaccca
     1381 gttgctattt agaatatata tttatcagtt ttagcaattt aaatcccata atcaatgtga
     1441 atataaaaaa tattattagc acaccgctat gttcactgtc acccgctcaa cagatcacgg
     1501 ttatcgttcg ttttttatac tgctcaggga taaaaaaagg cccctgttga aattgcaggg
     1561 gcctggtacg agcaagcatc atattgggcg acatgatgca acggtaaaaa tcatttggcc
     1621 tgatggcgtt caatgattcc tttcatttca gcaaccgccg ccccgtctac gatataacgc
     1681 gaatactcgt ccgcgtgttc atctgatgac atggacaatc cttgattacg ataacgcatc
     1741 ggtgaggctt gccacgctcc cgcggagcta tggacttcca gcactccggc atcgaggaag
     1801 tggtgcaggt tttctgcacg gactcctgct ccggccataa tgattggagc atcacgatgg
     1861 gcaataagtt ccataatttt tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc
     1921 agtacccggg caatgcccaa ttccgcaaga ttattgagtg tatataaagg gttagcgcac
     1981 atatcgaagg cgcgatgaaa agtcactgcc agcggaccgg cagcagccat tattttttcc
     2041 attcgtggca tatcgacatt cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa
     2101 cctaattcgc ggaccgtgcg cacatcctca agaatggcgg caaactcacc gtcgctgtag
     2161 caaaaatcac caccgcgtgg gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc
     2221 acggatttca gtacccccaa cgacggcgtt aagcccccct cttttggggc tgcgcataat
     2281 tcaactctgt ctgcgccgtt ttgctgcgcc gttagtgcac attccatgct gtaacagcaa
     2341 atttccagta atgccatttt tgctccttaa ttacgctgac tgctcgctgg caacgatctc
     2401 ttcaatggac cacggatgaa acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg
     2461 attcggtaat cgctcatgct caccttttgg tgaactggtt ttaactgaga tccccggtag
     2521 gctgagtcct tcatcagaaa gcaatgccag cgcacgggca ttcagcgttt ctggatcacc
     2581 cagcagaacg atttcaatat gttcccaacc ttcgtgtggg taacgttttt ccccgggcca
     2641 cggtagctcc acaatagaaa actgccagtg cgcaacctgt accggttcat gcaatttaaa
     2701 cagacaaatc ggtctgccat tgatcatatt ttctgacaaa agctcgccac actgttcaaa
     2761 cccgcgacgc cagcgttcag cagtggcgtt ttgatggcaa cgcaaagaaa tgtgatcggc
     2821 agtcagcgga gtgatattca accccagacg gagggaaagt tcatctaatg cgtgggtaaa
     2881 tcgcggtaaa tccgatgcaa tatcctgcag ctcgtcgata gattgccagt tcgccataat
     2941 cactcttcgt ctttcagtaa aagcgttaat ttaccctgtt gccctgcgcc aaccaaccgc
     3001 tgatttcacg ccgcttctga tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct
     3061 gacgaaaaca gccatttgca gtatactccc gccctaattt ctttaactgg tgcgggcaat
     3121 ttttgctcgc ttcatcaatg taaggtattc cggtgaatat tcaggctctt ctctcagaaa
     3181 aagtccgtca ggccatgatt gcggcaggcg cgcctgcgga ttgcgaaccg caggttcgtc
     3241 attcagcaaa agttcagttc ggcgactatc aggctaacgg catgatggca gttgctaaaa
     3301 aactgggtat gacaccgcga caattagcag agcaggtgct gactcatctg gatcttaacg
     3361 gtatcgccag caaagttgag atcgccggtc caggctttat caacattttc cttgatccgg
     3421 cattcctggc tgaacatgtt cagcaggcgc tggcgtccga tcgtctcggt gtttctacgc
     3481 cagaaaaaca gaccattgtg gttgactact ctgcgccaaa cgtggcgaaa gagatgcatg
     3541 tcggtcacct gcgctctacc attattggtg acgcagcagt gcgtactctg gagttcctcg
     3601 gtcacaaagt gattcgcgca aaccacgtcg gcgactgggg cactcagttc ggtatgctga
     3661 ttgcatggct ggaaaagcag cagcaggaaa acgccggtga tgaaatggag ctggctgacc
     3721 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     3781 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     3841 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga
     3901 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
     3961 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
     4021 ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     4081 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     4141 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
     4201 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     4261 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
     4321 aactggccga tctgctggat gaagccctgg aacgtgcacg ccgtctggtg gcagaaaaga
     4381 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
     4441 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     4501 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
     4561 ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
     4621 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     4681 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
     4741 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     4801 acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc
     4861 tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag
     4921 ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat
     4981 tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata
     5041 acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat
     5101 ctcttgccag gcttacgcag aagatacggt tatagaaaac gaccatgaca tttcagccat
     5161 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
     5221 cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta
     5281 tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgataccg aacaacttaa
     5341 cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat
     5401 cctttcaaat gaggacgttc aggatttaat gtacatcgtt ccacgtcgct tcgacaacgg
     5461 tattattctc tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc
     5521 gtatccacca ggccagcccg agcaatccgg cagtgaacag caaaaatacc aggttcggca
     5581 ttccaggcac caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt
     5641 tactgaaaag ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa
     5701 cgatgacccc cgcggcggta aaaatcacca gcgccggaat ttgtgccacc agaccatcgc
     5761 caatggtcaa tagcgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca
     5821 cgccgaccag caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat
     5881 cgccgcgaac aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca
     5941 cttcggagcg gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt
     6001 caatcgccat ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga
     6061 tacgcccggc acctttggta atgaccataa agttgatgat cacgagaatg acaaacacca
     6121 cgataccgat agcgaaattg ccaccaacga ggaagtgacc gaacgcttcg accaccttcc
     6181 ctgccgccgc cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa
     6241 gtgccagacg caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg
     6301 tgcgctgggt aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa
     6361 acaacaggtc gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga
     6421 tcaaaatcgg tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca
     6481 gcatcgcggc cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc
     6541 accggaagat gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt
     6601 tgccagaccc aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga
     6661 ccaatctccg catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg
     6721 ttattttcag cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc
     6781 actttcggtg cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg
     6841 ttattgacaa tgacatcagc tttcggcaca tcggccatca tccgacgccg tgcagcagct
     6901 cgctgcatct gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca
     6961 tcacgaatat cctgccgcga catacgcagc tttttcaggt ggctgaagat ttggaaaaag
     7021 acgtcaaatc ccaccattgg aatgacacca agcaccacca gcagtgcgca tagcccaacc
     7081 agatccatcg cattacccat ggcggtagtc ggagactcgg ccattaagcg catcatctgc
     7141 ggccagtgat gccagagata aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt
     7201 gccttaagca actccgcgcc agtctgtgcc gagaacatcc gtttaatgcc cggtagcggg
     7261 ttgagtttgg aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc ccccagcatg
     7321 accggagaaa tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg
     7381 gcttctctga tcagcagaat aatctgcccg aggatcagat tcgggtca
//
LOCUS       DAJTVK010000173         7239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000173 DAJTVK010000000
VERSION     DAJTVK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.175"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..337
                     /locus_tag="OO372_003872"
     CDS             <1..337
                     /locus_tag="OO372_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6343250.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="OO372_003873"
     CDS             337..666
                     /locus_tag="OO372_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6343251.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="OO372_003874"
     CDS             676..1374
                     /locus_tag="OO372_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6343252.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="OO372_003875"
     CDS             1379..2122
                     /locus_tag="OO372_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6343253.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="OO372_003876"
     CDS             2059..2661
                     /locus_tag="OO372_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6343254.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="OO372_003877"
     CDS             2722..6201
                     /locus_tag="OO372_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6343255.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="OO372_003878"
     CDS             6269..6868
                     /locus_tag="OO372_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6343256.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="OO372_003879"
     CDS             6932..>7239
                     /locus_tag="OO372_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6343257.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1726 c         2315 g         1561 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgt
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAJTVK010000174         7121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000174 DAJTVK010000000
VERSION     DAJTVK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.176"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..435
                     /locus_tag="OO372_003880"
     CDS             4..435
                     /locus_tag="OO372_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6343258.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OO372_003881"
     CDS             518..1420
                     /locus_tag="OO372_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR6343259.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO372_003882"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="OO372_003882"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6343260.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO372_003883"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="OO372_003883"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6343261.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO372_003884"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="OO372_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6343262.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="OO372_003885"
     CDS             complement(5330..6037)
                     /locus_tag="OO372_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6343263.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO372_003886"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="OO372_003886"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6343264.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="OO372_003887"
     CDS             complement(6895..7098)
                     /locus_tag="OO372_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6343265.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1991 c         1853 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAJTVK010000175         7062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000175 DAJTVK010000000
VERSION     DAJTVK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7062
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.177"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..302
                     /locus_tag="OO372_003888"
     CDS             <1..302
                     /locus_tag="OO372_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6343266.1"
                     /translation="QRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVP
                     SSAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(391..903)
                     /locus_tag="OO372_003889"
     CDS             complement(391..903)
                     /locus_tag="OO372_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6343267.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1098..1862
                     /locus_tag="OO372_003890"
     CDS             1098..1862
                     /locus_tag="OO372_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343268.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1813..3216
                     /locus_tag="OO372_003891"
     CDS             1813..3216
                     /locus_tag="OO372_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR6343269.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3216..4682
                     /locus_tag="OO372_003892"
                     /pseudo
     CDS             3216..4682
                     /locus_tag="OO372_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4573..5316
                     /locus_tag="OO372_003893"
     CDS             4573..5316
                     /locus_tag="OO372_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6343270.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5320..6540
                     /locus_tag="OO372_003894"
     CDS             5320..6540
                     /locus_tag="OO372_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6343271.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6544..7050
                     /locus_tag="OO372_003895"
     CDS             6544..7050
                     /locus_tag="OO372_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343272.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1873 a         1685 c         1934 g         1570 t
ORIGIN      
        1 gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct gatgcgaaag
       61 ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc gtcaaaggaa
      121 aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc aatgatgcca
      181 ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac ggaatcatca
      241 gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc cttggggggt
      301 agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga atgggggaag
      361 ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca cgaagctaaa
      421 actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg tatacacttt
      481 cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca gcacggcgat
      541 aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc gaacccgttc
      601 tattgtattg gaacggcttg atattataca tgcaagttga gggatatgcc caattattat
      661 accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg tgattccagc
      721 ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca taattgatgc
      781 tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta taaaaagaag
      841 cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta tccactccat
      901 caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga cttaatttag
      961 gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg gagcgtcgca
     1021 ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga taacagtaaa
     1081 tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg gcataccggg
     1141 ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa ggggctatca
     1201 ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt tgcaaaaatg
     1261 gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc cttacacaaa
     1321 aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc ggaatgcgta
     1381 ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa cacggtggtt
     1441 atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg ttgacgctcg
     1501 aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat attggtcggt
     1561 ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg attgaaaata
     1621 tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc atcgttggca
     1681 cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc gccactgata
     1741 aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat ggcaatggag
     1801 agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc ggctttacgt
     1861 agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc atcgtcaaaa
     1921 tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct gcgtttttgt
     1981 tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct caaaattcag
     2041 atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat cattaaccgg
     2101 gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga aattaagtca
     2161 ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga ataccagtgg
     2221 aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat attcaacccc
     2281 ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga aggcactctc
     2341 atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct taaggttatc
     2401 gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg cgttccggag
     2461 tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt ggatgcgcac
     2521 aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt ggctgacagt
     2581 ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg ggccgacgaa
     2641 tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca ggcggcgctg
     2701 gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc cggtgctaaa
     2761 ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg tgtgaattac
     2821 cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa cggcatcccc
     2881 aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc tgaccgtttc
     2941 agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga gctgatcagc
     3001 atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac acctcatcgt
     3061 gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc aaaacgcgag
     3121 ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat cgatacatcg
     3181 ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa caagccctgc
     3241 ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc gtagggatgc
     3301 agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt cgtaaccgca
     3361 tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc gatgctatcc
     3421 cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca aaagatcgtg
     3481 gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat gacgtgctga
     3541 aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg taggcaccaa
     3601 tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt ctcccgctcg
     3661 accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg ccggacctgg
     3721 gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc tggcgcattc
     3781 atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag aagatgaccg
     3841 agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc gcgttcgaca
     3901 gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc tacagcgtgg
     3961 agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg ctgaagaata
     4021 tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac tcgcgggata
     4081 agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg cagtttgtag
     4141 aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag tccccgaaag
     4201 gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt tcgttgcagg
     4261 agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt tcggaacttg
     4321 gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa atgtctgatg
     4381 tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg ctgggtgatg
     4441 gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac gtaaccggca
     4501 tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg ccgcccggca
     4561 tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta tcgaaccagc
     4621 ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga tggttcaaat
     4681 gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat caccccctgg
     4741 gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga gaaagtttgg
     4801 cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag cgctcctgtg
     4861 ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc tctgcctctc
     4921 gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt tgtcaccggt
     4981 ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc taagtctagg
     5041 gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga tatggcccga
     5101 gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg cgatgtccga
     5161 gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc aactttggac
     5221 ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga gatcgtgttt
     5281 gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt ttttgagacc
     5341 aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa agacgtgccg
     5401 ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact gaaacccgat
     5461 aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc ggccacgctt
     5521 gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa cgggaatgtg
     5581 cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca gaatgtccgg
     5641 cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa agacgaaagc
     5701 gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga cgcggagtac
     5761 gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca tgtggctctt
     5821 gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac aatggcaaat
     5881 caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga tgccgacacc
     5941 atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg ggatttgccg
     6001 cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga caaagcacca
     6061 gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt actgaaagcc
     6121 ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc tgacgataat
     6181 ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga ctcagcctgg
     6241 cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc gaccgcgttc
     6301 aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt cggtgatgcg
     6361 gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt gtctgaggtt
     6421 gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa catgggcatg
     6481 accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg taaagggtaa
     6541 aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc tgtctctcgc
     6601 ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt cgctgcctat
     6661 ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga tggtgatacc
     6721 gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca gccggacatg
     6781 gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg tggctacgtg
     6841 accgttaatc tcggttctga ttttgatgcc agcactatca aaaaaggcga tccggtatac
     6901 gttgtcgtct ccactgatga atccatcaaa gtgccgctgg gtggattcat gtccacgtca
     6961 gtcagtggca aaaacgtggt gctgaccaac gctgaattca caggtgccgg tgatgctgac
     7021 ggcaatgcag aaatttcctg gaagatttaa ggaacagacg aa
//
LOCUS       DAJTVK010000176         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000176 DAJTVK010000000
VERSION     DAJTVK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.178"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="OO372_003896"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="OO372_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OO372_003897"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="OO372_003897"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR6343273.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OO372_003898"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="OO372_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6343274.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="OO372_003899"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="OO372_003899"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6343275.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5493)
                     /locus_tag="OO372_003900"
                     /pseudo
     CDS             complement(4172..5493)
                     /locus_tag="OO372_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5501..>6795)
                     /locus_tag="OO372_003901"
     CDS             complement(5501..>6795)
                     /locus_tag="OO372_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6343276.1"
                     /translation="IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQ
                     TFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSE
                     FIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSA
                     ERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQ
                     LNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDD
                     FGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVES
                     EDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1803 a         1697 c         1552 g         1743 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagcgtgt tgataagatc aattgccacg ctacgccgat attcagcaat
     5341 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggctataca cccgttcctt
     5401 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
     5461 gactgtaagt acgaacttat tgattctgga catacgtaaa ctactctttt actaattttc
     5521 cacttttatc ccaagcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
     5581 cacatcctaa tttaattaat tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca
     5641 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
     5701 cgaaaatatc gccggtaaat tgcgagtcca cctttatgct gtctggatta atttcataaa
     5761 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
     5821 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
     5881 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
     5941 gctcatgaaa gtgcggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
     6001 catccttcag cgacgcacgc tgactaaaat gtctgaattc acccattgcc gttttaataa
     6061 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aacttcagcg
     6121 gtgaaatgat cccccgctcc gcagaaaccc aacggcacaa cgcctccgcg cccagaatgg
     6181 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
     6241 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
     6301 cacagaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
     6361 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
     6421 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
     6481 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
     6541 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
     6601 tgcgttcatc aacaccaaac accgcttcca cggcttccag accacacagt ttgatgatcc
     6661 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
     6721 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
     6781 tcatttccgc gatcg
//
LOCUS       DAJTVK010000177         6713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000177 DAJTVK010000000
VERSION     DAJTVK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.179"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            394..1269
                     /locus_tag="OO372_003902"
     CDS             394..1269
                     /locus_tag="OO372_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR6343277.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1277..2053
                     /locus_tag="OO372_003903"
     CDS             1277..2053
                     /locus_tag="OO372_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR6343278.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2222..4483
                     /locus_tag="OO372_003904"
     CDS             2222..4483
                     /locus_tag="OO372_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6343279.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4552..5727
                     /gene="senB"
                     /locus_tag="OO372_003905"
     CDS             4552..5727
                     /gene="senB"
                     /locus_tag="OO372_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR6343280.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6253..>6713)
                     /locus_tag="OO372_003906"
     CDS             complement(6253..>6713)
                     /locus_tag="OO372_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCR6343281.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
BASE COUNT         1899 a         1555 c         1541 g         1718 t
ORIGIN      
        1 tgtggctgat acgcacacat taacgtacgt tttcgttcca ttggccctca ccccccgata
       61 acgacccagg tagcgcgccg tattcccccc gccggccgtt ttcttcgaca tgtacatcag
      121 gcgcggaact catcggggcg cgggaatgaa atcccattca gactccggat agattcaagc
      181 aagcccactt atcgtcaaaa tcggtgttgc aaaaacggga gtgaccatag atccgttttc
      241 caagcggacc ccataatatc ggctggtaaa catcactcgc tagtgcattc tttttgttgg
      301 taatgataat catttacatt cattctttga tgttttatca taattaatgt tttgtcaata
      361 aatatatgga aacatattgt ttcagatggg aatatgagaa agttcatcct tatttctatg
      421 ataacgttag ctagtgttag tctgggagcc tgcagacaga atgtcaccat aaagcaggat
      481 gcgccagggc agaaagcttt cctgactgac gggcagattt acgatttgca cagtggcaaa
      541 attatttcta gcagcgagct tttggcggat cttgctaccg ctcgacatct tattattggt
      601 gaaaaacacg ataatgccga gcatcatcag attgagcttt ggcttattca aaatttgctc
      661 atccagcgac cacaggggag tgtgttgctt gagatgttga cgtcagagca acagccccga
      721 gttaaccaag ttaaatgttg gctgaaagat aatcctgtgg ttcgagatag ccgtgttcag
      781 gaactactta actggcaaaa agggtggtcc tgggagatgt atggcgacat cgtcatgcag
      841 ctattacgtg ggccttaccc gctgctcaat gccaacattg gccgggagca gatgttggca
      901 ctgtacaaaa aaaacgaatt tcctaaagga aaaaaatcca ctgcacctgt ggtacaagaa
      961 gctctgcgtg aaaccatcat atctatgcat gaaggcaacc ttgaaagcca gcaactgacg
     1021 tcaatgctct ctattcagca gcagcgggat cgctacatgg cccgccagct tctgagtgcc
     1081 ccggtaccgt cgttgttgat tgcaggtggt tatcacgcgt caaaaagcat gggcgtaccg
     1141 ttacacatgg aagatcttgc caccggcacc catccagtgg tgttgatgct tgcagaaaaa
     1201 ggaatgaaca tcacagtaga tcatgctgac tacgtgtggt ttgtagcgcc agatacaaca
     1261 aaaaggtaaa ccatggatga tgaatattct ccacttctca cagtcggtaa aatggagcag
     1321 ctggtttatc tgttctctac tgcttcatgg actgattttc cttgcgttta tctggcgttt
     1381 ttccgaagtt cagcccgcta tgtctcctgc accggcgatc atgctgcaat gggcagaaat
     1441 tatagaggct ccttcttctc cactttcgct tccggtggga atcgctcaac aggagtccgc
     1501 agtgacagaa gaaaagcaac aaactgagga cagacaacaa cgtcctgtca ctgaagacag
     1561 tgatgcaacc attgaaataa ctaggaagaa aaagtcatcc gatggagaaa agaaaaagac
     1621 acgtccacct cgtaagataa aggctcaaac cagcgacagc aaccccacag ccgtttccag
     1681 taatgctgca cctcaggctc tggtcgaatc gtcccgtatt gctgcgcctt ttaacagtga
     1741 ctcaacaaag cgagataatt ctgaggcatc ttgggaaagc agagtaaaag gccacctaaa
     1801 ccgttataaa cgttaccctg gcgatgccag aaaacgtgca cgaaccggca cagccgtcgt
     1861 gacttttacc gtaaacacgg aaggcactat tgtcagtagt ttcttggaaa tctcttcagg
     1921 aaccctttct cttgaccgcg aagctattgc tgttttagaa cgcgctcaac cacttcccaa
     1981 accaccgcct gaaatattgg agggaggatt atttaaagta aaaatgccga ttacctttaa
     2041 gttgaaggag taaagggata attggccgtt caagctcatt taactgaaat ttaattttca
     2101 tttttatgct gaacatttta tcaaactttt ccactgtcga aaaaatcaaa aagacagtag
     2161 aacataatta ataaacaaat agttatactt atctttatta atattatacc caaggaaatt
     2221 tatgaacgtt ataaaactgg ctatcggctc aggcatatta ttgctcagct gcggtgctta
     2281 ctcacaatcc atcagtgaaa aaactaattc cgacaaaaaa ggagcggcag aattcagtcc
     2341 gctcagcgtt tctgtcggga agacgaccag tgagcaggaa gctctcgaga aaacaggcgc
     2401 gaccagttcc cggacaacgg acaaaaacct gcaatcactt gacgcaacag tgcgtagtat
     2461 gcctggtact tatactcaaa tagatcctgg tcagggagca atcagtgtga atattcgagg
     2521 catgagcgga tttggtcgtg taaacactat ggtcgatggt attacccaga gtttttacgg
     2581 aacctctacc tccggaacaa cgacgcatgg ttcaactaac aatatggctg gcgtacttat
     2641 agatcctaac ttactggtag cagttgatgt tacacgcggt gacagcagtg gctctgaagg
     2701 gatcaacgcc cttgccggta gtgcaaatat gcgtactatt ggcgttgacg atgtaatatt
     2761 taacggtaat acatatggcc ttcgttcacg tttctctgtc ggtagtaatg ggctgggacg
     2821 cagcggaatg atcgcccttg gtggaaaaag cgacgctttt acggatacgg gaagcattgg
     2881 cgttatggct gctgtgagcg gcagttctgt gtactctaat ttctcaaatg gttctggaat
     2941 taacagcaaa gagtttggtt atgataaata tatgaagcag aaccccaaat cccaactgta
     3001 taaaatggat atcagaccag acgaatttaa cagcttcgaa ctttccgctc gaacctatga
     3061 aaataaattt acacgtcgtg atataaccag tgacgactat tacattaaat atcattacac
     3121 ccctttttct gaattaattg actttaacgt aacggccagt accagtcgcg gtaatcaaaa
     3181 gtatcgtgat ggctcgctgt atactttcta caaaacctca gcgcaaaatc gttctgacgc
     3241 gctggatatc aacaatacca gccggttcac tgtcgcggac aatgacctgg agtttatgct
     3301 gggcagcaaa ctgatgcgta cccgctatga ccggaccatt cactcagcgg cgggcgaccc
     3361 gaaagcgaat caggaatcga tcgagaacaa tccgttcgca ccctccggcc agcaggatat
     3421 ttcagcgctg tataccgggc tgaaggttac gcgcggcatc tgggaggcag atttcaatct
     3481 caactacaca cgtaacagga tcacagggta caagcccgcc tgcgattcac gcgttatctg
     3541 cgtgccacag ggtagctacg atattgacga taaagagggt ggcttcaacc cttcagttca
     3601 gctttctgct caggtaacac catggcttca gccgttcatt ggctacagca aatccatgcg
     3661 cgccccgaac atccaggaga tgttcttctc taattcagga ggcgcatcca tgaacccatt
     3721 cctgaagcct gaacgtgcag aaacctggca ggcgggtttt aacattgata ccagagattt
     3781 actggtcgaa caggatgccc tgcgctttaa ggctctggcg taccgcagca ggatccagaa
     3841 ctacatctac agcgagtctt atctggtttg ttctggaggt cgtaaatgca gtatgcctga
     3901 ggtgattggc aatgactggg agggcattag cgatgaatac agcgacaata tgtacatcta
     3961 cgttaactcg gcaagcgacg ttatcgcaaa gggcttcgaa ctggagatgg attatgatgc
     4021 aggttttgct tttggccgac tctctttcag ccagcagcaa acagaccagc caacctccat
     4081 cgccagcacc tactttggcg caggggatat gaccgaactg cccagaaaat acatgacgct
     4141 ggatactggt gttcgcttct tcgataacgc gttgaccctg ggcactatca taaaatacac
     4201 aggcaaggct cgtcgcctgt cgcctgattt tgagcaggac gaacataccg gcgcaataat
     4261 caaacaggat ttgccgcaga tcccaacgat tatcgatctc tatggtactt acgagtacaa
     4321 ccgcaaccta acactgaaac tttcggtaca aaacctgatg aacagagatt attcggaggc
     4381 gctgaataag ctcaacatga tgccaggtct tggtgacgag acccacccag ccaattccgc
     4441 gcgtggcaga acatggatat ttggcgggga cattcgtttc tgatgacaat ttccgctagg
     4501 ttcagacaat acgttttctc tcttatgtca attttattgc aggaacgaaa aatgaatatt
     4561 ttcactttat ccaaagcacc gctatacctg ttaatttcac tatttttacc cacgatggcc
     4621 atggctatcg atccacctga acgcgaactt tcgcgatttg ccctgaaaac gaattacctt
     4681 cagtcccctg atgaaggcgt ctatgaactg gcgtttgata atgccagtaa aaaggtgttt
     4741 gcagcagtca ccgatcgtgt aaatcgtgaa gccaataaag gctatctgta ttcgtttaat
     4801 tcagattcgc tgaaagtcga aaataaatac acgatgccat accgggcatt ttcgctggcg
     4861 ataaatcagg ataaacatca gctctatatc ggacacaccc agtcagcgtc cctgcgtatc
     4921 agtatgtttg acaccccaac cggcaaactg gtaagaacca gcgacaggtt aagttttaaa
     4981 gcggcaaacg ctgcagattc gcgttttgag cattttcgcc atatggttta cagccaggat
     5041 tccgataccc tgtttgtgag ttatagcaat atgctgaaaa cggccgaggg catgaagcct
     5101 ctgcataagc tgttaatgct cgacgggacg acgcttgcct taaaaggcga ggttaaggat
     5161 gcttacaaag gtacagcgta tggtctgacg atggatgaaa aaacacagaa aatctacgtt
     5221 ggcggaagag attacatcaa cgaaattgat gcgaaaaatc agacgctgct gcgtaccatc
     5281 ccgttgaaag atccgagacc acaaatcaca agtgtgcaga atctggcggt ggactccgct
     5341 tctgaccgtg cctttgtggt ggtattcgac catgacgatc gttccggtac aaaagatgga
     5401 ctctatattt ttgacttacg cgacggtaaa cagcttggct atgtgcacac aggagccgga
     5461 gctaacgcgg tgaaatacaa tccgaaatat aacgaactgt atgtcaccaa cttcactagc
     5521 ggcaccatca gcgtagtgga tgccaccaaa tacagcatca cccgtgaatt taacatgccg
     5581 gtctacccaa accagatggt gttgtcggac gatatggata ccctttacat tggcatcaaa
     5641 gaaggcttta accgcgattg ggatcctgat gtgtttgtgg aaggagctaa agaacgtatt
     5701 ctgagcattg atttgaaaaa gtcgtgatcg gggggaaaaa acatgggata aaaactccgt
     5761 tattccacta aactggccgg actaggaaaa gcaaacatct ctgtcctgcc ggtacgcctg
     5821 ttacgccgct ccatgaggct gaggaatctc cagaaaacga gacaggcaca gcattgtacg
     5881 atcaactgat ttgtgccaat aattaaatga aatcacagct atgcctgttc atggggttct
     5941 aggccggaac cccagatttt tccgattgtt tgttttctaa ctgattattc agtagtttta
     6001 gagggctgac gctcagtgga acgaaaactc acgttaagag gttttgacta tgataaaagt
     6061 caaagtaggg gtgagtttgg atatggaaat tattgtacta taaggagagc atgaaaaccc
     6121 ctggggcagt cactgaatga gggaccagca gcggtaaaat aaccagcata atttctgcat
     6181 ggtcgatttt tataccgtga caggctgttt tttatacttg gggtcagttt ggatatcgaa
     6241 aattattgta cgttaaggtt gttttttgac cttgtcgatc ttaactgatc tcaaacgacg
     6301 aaacttacct gaagtcggta tccggctgtt tttccagata taatcgcctg tcagattgat
     6361 atgttcccaa cctaatggtg ataaatgcga taatagttga gcatttattt tcatgccctt
     6421 acgcttcaga gcatcaaccg ctctttccat gtaaactgtg ttccataagg aaatagccgc
     6481 cgtcaataaa gtcaatccac tggcgcgata actttgattt tccggtttcc gatctctaat
     6541 ctcccccaat cgatgcatga atacagcacg ggccagagca ttacgggctt ctcctttgtt
     6601 cagcccggcc tggacacgtc gccgcaatgc cggatcccga aaccagtcca gcataaacag
     6661 tgttcgttct atacggccaa tctctctcag agctttagcc aacccatttt gtt
//
LOCUS       DAJTVK010000178         6622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000178 DAJTVK010000000
VERSION     DAJTVK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.180"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(24..605)
                     /locus_tag="OO372_003907"
     CDS             complement(24..605)
                     /locus_tag="OO372_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343282.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OO372_003908"
     CDS             complement(652..4509)
                     /gene="sigA"
                     /locus_tag="OO372_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6343283.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4870..5874)
                     /locus_tag="OO372_003909"
                     /pseudo
     CDS             complement(4870..5874)
                     /locus_tag="OO372_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5859..6236
                     /locus_tag="OO372_003910"
                     /pseudo
     CDS             5859..6236
                     /locus_tag="OO372_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6275..>6622)
                     /locus_tag="OO372_003911"
     CDS             complement(6275..>6622)
                     /locus_tag="OO372_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6343284.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1644 a         1471 c         1494 g         2013 t
ORIGIN      
        1 acctcaaaat accgggacag agatcaggcg tgattaatct gcagcatttt taataacaga
       61 agtactgagc ttactttacc tttgatgatg taatagttat ttccgtcggt actcattttt
      121 ccctgaaata atgtcacgga tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc
      181 atatttttaa tgagctgaga tccgggtggc aaggggtatt tgctgtcaga tactgggcga
      241 ctgagtctga tgtggcgcgt atacagcccg ttttcgtaca gggtggcctc tgttatggtg
      301 tcagtgacca gcatgtggga gacaatgctg tattcttttt taaagcactc accagaaacg
      361 gtgattgtaa tgataatggt cttctcgatc gagatgtaaa tacccgtttc gttatactgt
      421 ccgtatcgga accatgagaa ctcagttgca ttccagccat gctccagaat atgtgcattt
      481 ttttgcctga tagtttcttt ttttattcgc tcatgtttaa ggcataacag gcagataaat
      541 aatgtcagaa aaagaaagct aataatgaga gcaagagcga tatagtctgt cacagggtaa
      601 tacatattct ctacggtaga agaagggccg caaacgcggc ccgggctgtt atcagaaaga
      661 gtaacggaag ttggcgttga tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc
      721 aaactcaaga ccgaagcgaa gattatcgcg aatttcggcg ttgagaccaa cattcatgag
      781 aatacgaccg tctttttcac ctttgatacg tttctcaccg gacgcatcac gcagtacggt
      841 ttcaccgttg gcaaacaggt caaactggta gccaaggccg gcgcgggctg tgactttcca
      901 gtccttaccg gagaaggatt tacccacatc aacaccggta cgcccaatca gcggattaaa
      961 gtccttatcc ttcatggtca cgctcattcc gcgatcctgc cagtcgaacc gtttacctga
     1021 tacggcccca taaaccagtt ctgcctgcgg ctcaatccag gagtcttctg ttacatgata
     1081 gcggtagcct gcttcagcac ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag
     1141 tccagcaaag gtcgccgagt actcattatc atggtgaaca tacttactaa tcaggtcgat
     1201 ataggcacca gagtcaaata tagcggaagc atacagacct gccccgacgg acttcgtctt
     1261 gccactgaat gcattactgc tggcatgact gtcggtatac gtcatagtca gaccagtgaa
     1321 aaggtcaagt ccatccagct catgtttttt atccacacca atctgcacat gggtgtagtt
     1381 gtcactgtat ccactagaag ctgaacctgc tccgctcatg atgcgggccc atgcaccggc
     1441 ctcgccattg gtgtcacgca gatcccccat tcgtttattc aggttgttga cttctgtcag
     1501 gaaggctttg taattaccgg acatatacga ttttgctgca ccggaagatg ctgtattttc
     1561 agccacctta tatccggtca gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat
     1621 cacaggagat atgttgctga aacctatggc ctgagtactg gctttaaata tctctttatt
     1681 gcttcctttt ggggctgtta tcaggtcaac gtttaatcct gagttattat ttcccacttt
     1741 ctgtatatcg accgacagac tgttattgct tccggttaac tgatttttaa caatgagttt
     1801 atccgattga tttacagact ggctcagatc agcccgcata atcacagcag tattagtggc
     1861 atccagtgtg tttactgtca cagtatggaa acccgaattc tctatgttac taaacaacaa
     1921 aagactgtcg cgggttttta atgattgcag ggctgactgt ccgtttactc tccataaggt
     1981 gttatacatg ctggctgagg cagcaggagc atcaatacct ccggtataag aagtatcaaa
     2041 gccacccagc aaacctgtag ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt
     2101 atactggtca acaccaaaca gaatagatgc ttttttgtct gattttatat taccattgac
     2161 ccaggcataa ttttgagcct ttaacgtggc accatcttct gtcatattat atccatctga
     2221 catatagatt gccgggctgt atttattatt ttctgtaggg gtgcctgtta atgtcagggt
     2281 tccggccagt tcaatagctt tatctacata catttttccc tgggcggtaa cagtagctcc
     2341 tttgttgata atcaggtctg tattaattaa ggataatgca ccagtcatag taacagttga
     2401 tttatcacca acagttattt tgctgttttg agcactcaga ctgccgttga aactactttc
     2461 gcctgctgaa tccccggagc gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt
     2521 tcttcctgag aacatgtcaa tgtatgcagt tttatttccc agaacaattt gagagttttt
     2581 agcctctatg tgtcctttta cattagcatc ccggcctata ctcagggttg cgttttctaa
     2641 atttaatttc cggaaattaa atttacgact ttcccagtcg ggctgggaaa aagaggctat
     2701 ctgattgttg gacttatatt ccgtattgta acgtttattt acagtgcttt cctggtcctt
     2761 tatggcagca gaatagtctt tttgacataa aaaaggaatt ggacagttgt tattgccttc
     2821 tttaaatatg gcatgttctg tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga
     2881 gatatcgttt ttgatatcga ctgagccatc aaagatcaga ttggcattat tgttatgttg
     2941 ctgggtagta ttgataatat gatttatgct tatattacct gatacattcc catggtacat
     3001 atatgcatca gtattagtaa gtgataatgt tgattgcttc acgtttgagt tcgttacggt
     3061 tgttcccgca tctgttgctg cgattttctg aaatgactgg tcatagccgt ttagatccag
     3121 tgttccgcca tttctggtaa aatagatttc gccattaccg ctttcgctta aagcgtcttt
     3181 ggcatttatt tttaccgtac ctttacctcc ggccatgtaa attttattga aggctttttc
     3241 agcactaagg atgacagtcc cattacctat tttaaggtta tttccctgtg ctatttttac
     3301 atccagagta ccactaccta ttttatgcag attatctccg gcaacgccct taacattcca
     3361 ttcaacggtg gtattattat caatatctat cccggcacct ttataagact tatcttttcc
     3421 gtacacttta tatgtctggt tcttatcgaa tataagcccg ccaatgccaa gatcggtatc
     3481 ctgcttgagc gttaattccc ctcctcctga gacagacagg tccttatctt ttataatcga
     3541 tgataactct tgttttttac catccagctg catagtgttt gtgaaattct tttttaaatt
     3601 gtttaccaga atattattaa ataacgagta gtttgtccag gctatattgg tactactgag
     3661 aaaaccaact gtactggtaa caccatggaa aacccatttt tgttgttctt tatcatatag
     3721 ataagaacca gaaccactat cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt
     3781 ataaatgtaa ctcttgtttt tccagtccag attaaaaaga cttgcgctta acaagtcggg
     3841 attatagttt tgtccaaaca gatattgttt gccgtttaat atcgttgagg ttgttccggc
     3901 acctgctctg aagccaatta tttgttcctt accgttgtag tttacgccat atcttttcat
     3961 cgcatcttcg ggagaaagtg agaagtcggc actctccgta acccctgttg tttccacgac
     4021 gaatttattc agacgatgaa ccgaaaagtc gccagatgat actctgttac ttgctttata
     4081 tgctgtctga tcccatgact gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc
     4141 agtaacactg tacgcacctc caattgatgt tgttgcacct ttgttggaaa cagcagaaaa
     4201 atcaggtaca ggtagttctg gaaaatgaaa tatctttcca tctttcatca ttaattgaac
     4261 atttgttgca cccggtctga actctcctct gttttgagca aggtcaagat aatcgcgggt
     4321 ccatatatta tttatatcca gcattgctgc aaatgctgtt tcgccataac ttccatatat
     4381 tgaggataaa ataataccga ggataacttt ctttcttgat gtaccgacac taactttccg
     4441 ggtcagttca gaaacagcaa ctaatccacc tgtaatatga ctatatttca gtgaataaat
     4501 tttattcata cgtgaactcc gatatttctg agttgtggtt atgggatagc atttaattac
     4561 aacggggaac tatcagtcat ggtacgtaac tgataagggt ataatgataa tcactgtcag
     4621 ttatatttgt atgacagatg taacaaaatg aaaaaaaaga taagcacaag tgattgattg
     4681 gtcagatatt gatcacttgg tgtttgtatt ttgaactttg cgatcaaaat ttgatctttg
     4741 ttgtttccgg tcaatccttt acaggttttc tgtattatcc gggggagtta agtctgtcga
     4801 tgtccgacag acttttgatg atggaaacaa catgcagcga taatgtgcat gctgttttgc
     4861 aggtggaact cagaaggtga actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc
     4921 gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt gagatcccca ccccggccgt
     4981 gaatgcactg ccgtcgttac cggccagatt ctgacggtaa ccggcgcgca gctgtgccca
     5041 tttccaggca ttaaattctg cgccaatggc ggcaaactga cggttgctgt cggaggtgaa
     5101 accgctggcc ggggtcagat ccacatcaaa tgcggtggtg aacatcgcgt tgtgccagga
     5161 gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc gtgataccgt tcacttcttt
     5221 tgtctcaata ctacggggga tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc
     5281 caggtccgta ctggcaccga tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc
     5341 gcctttgaag gcgctgctgt cataatttct gaccagcacg ttgtagttga acaggtcgac
     5401 gcgctggaat ttggggggga agcccagaga ccatttctgc ccggccgttt ccagttccgt
     5461 agccagagca atacccacat cagtgatcag tgccgcgcgg gcaaaggcct gtgatgtcag
     5521 gcggcttttg tccacctcct gcccggtgct gtttgccacc tcttccaggt aagtgaggtc
     5581 agcatcagag actttaccgt cgacactgac ggtgccgtgt gatttaacca tcagtgcggc
     5641 agagaggcga tcaccgggaa gggccgccac agcagaaact cccagctgtg cggcggcatg
     5701 tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg
     5761 gttactttcg atggcgcggt cgaaggcctt ccactcatca ctgatccggt cggcgttgtc
     5821 ggtgatatta tccggattgg agagctgcgc gccgacggac ggaagcagga tgctgggctc
     5881 atgcgcgacg caaaatccac gatgtatata tcagtactaa aagcgcgacg gcggaagaag
     5941 ctctgaaact aatcggtgaa ctgtacgcca tagagcacga tatacgcggg ttaccggtgt
     6001 ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct cctgacttcc ctgtataagc
     6061 tgatgcagga gaaagaacac acgttatcga agaaatgtcg tctgggagat gcgttccggt
     6121 atatcaggaa gcactgggct gcgttgtgca acttctgtga tgatggcctg gctgaggtag
     6181 ataataatgc agcagaaaga gcgcttcgtg cagtctgtct cgggaagaaa actttttcga
     6241 ggatcactca ttccgtgtct gactgcttca gggtttaatt ttgcttctgc tgactgaatg
     6301 atgattgggc agcaagtgca atatcccaca taaatccagt taactcacga gccaccgccg
     6361 taatggctac ttgtgatttt ttcccttttg ctgtcagagt tcggtaacga gaacacaatc
     6421 gttgttgagc tttccatgca atatctttgg aatattgtgt tacagagtcc ggtcggtaat
     6481 atataagcca tgctccaact tttgcaggtg tacgataaga ccaagcggct tcgtaaagca
     6541 gacgtctcag ttcgctgttt ccaacttttg ttattcctct gggacgaata ctgtttccac
     6601 tggaatattc acctggcacc ag
//
LOCUS       DAJTVK010000179         6537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000179 DAJTVK010000000
VERSION     DAJTVK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.181"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="OO372_003912"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="OO372_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6343285.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="OO372_003913"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="OO372_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6343286.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="OO372_003914"
     CDS             2408..3157
                     /locus_tag="OO372_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6343287.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="OO372_003915"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="OO372_003915"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO372_003916"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="OO372_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6343288.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="OO372_003917"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="OO372_003917"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6343289.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="OO372_003918"
     CDS             6018..6491
                     /locus_tag="OO372_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HCR6343290.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAJTVK010000180         6530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000180 DAJTVK010000000
VERSION     DAJTVK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.182"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..906)
                     /locus_tag="OO372_003919"
     CDS             complement(<1..906)
                     /locus_tag="OO372_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HCR6343291.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
     gene            1602..3887
                     /gene="nrdA"
                     /locus_tag="OO372_003920"
     CDS             1602..3887
                     /gene="nrdA"
                     /locus_tag="OO372_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6343292.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            4121..5251
                     /gene="nrdB"
                     /locus_tag="OO372_003921"
     CDS             4121..5251
                     /gene="nrdB"
                     /locus_tag="OO372_003921"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6343293.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            5251..5505
                     /gene="yfaE"
                     /locus_tag="OO372_003922"
     CDS             5251..5505
                     /gene="yfaE"
                     /locus_tag="OO372_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6343294.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OO372_003923"
     CDS             complement(5582..6208)
                     /gene="inaA"
                     /locus_tag="OO372_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6343295.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            6387..6530
                     /locus_tag="OO372_003924"
                     /pseudo
     CDS             6387..6530
                     /locus_tag="OO372_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1685 a         1685 c         1526 g         1634 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttataata taaggtaaat taacaaaatg
     1141 gcttcgcatt taacaataac cgaatagaaa acaaccattt cgacatcaac aatcgcttac
     1201 attcgcttat atattgacca caactgatac atcagattat gtgatgactc gtgcttagat
     1261 caatttttgc aatcattaac aaaaagatta ataaaccatc tatatcaatt tatccaacct
     1321 attctgccgt tcaagaaatc gccgaacagt tatttttaac aaatttttct cttcccattg
     1381 actttcccgg acaccttgtc tgacctaagg tgcgcgaaag ccactttttc cttcctgagt
     1441 tatccacaaa gttatgcact tgcaagaggg tcattttcac actatcttgc agtgaatccc
     1501 aaacataccc cctatatata gtgttctaag cagcttccct tactacaggt agtctgcatg
     1561 aaactattgc ggaaagaatt ccaaaaacag gtacgacata catgaatcag aatctgctgg
     1621 tgacaaagcg cgacggtagc acagagcgca tcaatctcga caaaatccat cgcgttctgg
     1681 attgggcggc agaaggactg cataacgttt cgatttccca ggtcgagctg cgctcccaca
     1741 ttcagtttta tgacggtatc aagacctctg acatccacga aaccattatc aaggctgccg
     1801 cagacctgat ctcccgtgat gcgccggatt atcagtatct cgccgcgcgc ctggcgatct
     1861 tccacctgcg taaaaaagcc tacggccagt ttgagccgcc tgcgctgtac gaccacgtgg
     1921 tgaaaatggt cgagatgggc aaatacgata atcatctgct ggaagactac acggaagaag
     1981 agttcaagca gatggacacg tttatcgatc acgaccgtga tatgaccttc tcttatgctg
     2041 ccgttaagca gctggaaggc aaatatctgg tacagaaccg cgtgaccggc gaaatctatg
     2101 agagcgccca gttcctttat attctggttg ccgcgtgcct gttctcgaac tacccgcgtg
     2161 aaacgcgcct gcaatatgtg aagcgttttt acgacgcggt ttccacattt aaaatttcgc
     2221 tgccgacgcc aatcatgtcc ggcgtgcgta ccccgactcg tcagttcagc tcctgcgtac
     2281 tgatcgagtg cggtgacagc ctggattcca tcaacgccac ctccagcgcg attgttaaat
     2341 acgtttccca gcgtgccggg atcggcatca acgccgggcg tattcgtgcg ctgggtagcc
     2401 cgattcgcgg tggtgaagcg ttccataccg gctgcattcc gttctacaaa catttccaga
     2461 cagcggtgaa atcctgctct cagggcggtg tgcgcggcgg tgcggcaacg ctgttctacc
     2521 cgatgtggca tctggaagtg gaaagcctgc tggtgttgaa aaacaaccgt ggtgtggaag
     2581 gcaaccgcgt gcgtcatatg gactacgggg tacaaatcaa caaactgatg tatacccgtc
     2641 tgctgaaagg tgaagatatc accctgttca gcccgtccga cgtaccgggg ctgtacgacg
     2701 cgttcttcgc cgatcaggaa gagtttgaac gtctgtatac caaatatgag aaagacgaca
     2761 gcatccgcaa gcagcgtgtg aaagccgttg agctgttctc gctgatgatg caggaacgtg
     2821 cgtctaccgg tcgtatctat attcagaacg ttgaccactg caatacccat agcccgtttg
     2881 atccggccat cgcgccagtg cgtcagtcta acctgtgcct ggagatagcc ctgccgacca
     2941 aaccgctgaa cgacgtcaac gacgagaacg gtgaaatcgc gctgtgtacg ctgtctgctt
     3001 tcaacctggg cgcaattaat aacctggatg aactggaaga gctggcaatt ctggcggttc
     3061 gtgcacttga cgcgctgctg gattatcagg attacccgat cccggccgcc aaacgtggag
     3121 cgatgggtcg tcgtacgctg ggtattggtg tgatcaactt cgcttactac ctggcgaagc
     3181 acggtaaacg ctactccgac ggcagcgcca acaacctgac gcataaaacc ttcgaagcca
     3241 ttcagtatta cctgctgaaa gcctctaatg agctggcgaa agagcaaggc gcgtgcccgt
     3301 ggtttaacga aaccacttac gcgaaaggga tcctgccgat cgatacctat aagaaagatc
     3361 tggataccat cgctaatgag ccgctgcatt acgactggga agcattgcgt gaatcaatca
     3421 aaacgcacgg cctgcgtaac tctacgcttt ctgccctgat gccgtccgag acctcttcgc
     3481 agatctctaa cgccactaac ggtattgaac cgccgcgcgg ttacgtcagc atcaaagcgt
     3541 cgaaagacgg tattttgcgc caggtggtgc cggactacga gcacctgcac gacgcctatg
     3601 agctgctgtg ggaaatgccg ggtaacgatg gctacctgca actggtaggt atcatgcaga
     3661 aatttatcga tcagtcgatc tctgctaaca ccaactacga tccgtcacgt ttcccgtcag
     3721 gaaaagtgcc gatgcagcag ttgctgaaag acctgctcac cgcctacaaa ttcggggtca
     3781 aaacgctgta ttatcagaac acccgtgacg gcgctgaaga cgcgcaggac gatctggtac
     3841 cgtcaatcca ggacgatggc tgcgaaagcg gcgcatgtaa gatctgatat tgagatgccg
     3901 gatgcggcgt aaacgcctta tccggcctac ggctcggttt gtaggcctga taagacgcgc
     3961 cagcgtcgca tcaggctccg ggtgccggat gcagcgtgaa cgccttatcc ggcctacggc
     4021 tcggatttgt aggcctgata agacgcgcca gcgtcgcatc aggcacagga tgcggcgtaa
     4081 aatgccttat ccggcattaa actcccaaca ggacacactc atggcatata ccaccttttc
     4141 acagacgaaa aatgatcagc tcaaagaacc gatgttcttt ggtcagccgg tcaacgtggc
     4201 tcgctacgat cagcaaaaat atgacatctt cgaaaagctg atcgaaaagc agctctcttt
     4261 cttctggcgt ccggaagaag ttgacgtctc ccgcgaccgt atagattacc aggcgctgcc
     4321 ggagcacgaa aaacacatct ttatcagcaa cctgaaatat cagacgctgc tggattccat
     4381 tcagggtcgt agcccgaacg tggcgctatt gccgcttatt tctattccgg aactggaaac
     4441 ctgggtcgaa acctgggcgt tctcagaaac gattcattcc cgttcctata ctcatatcat
     4501 tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat atcgtcacca acgagcagat
     4561 ccagaaacgt gcggaaggga tctccagcta ttacgatgaa ctgatcgaaa tgaccagcta
     4621 ctggcacctt ctgggcgaag gcacccacac cgttaacggt aaaaccgtaa ccgttagcct
     4681 gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc gttaacgcgc tggaagcgat
     4741 ccgcttctac gtcagctttg cttgttcctt tgcctttgca gaacgcgaac tgatggaagg
     4801 taacgccaag attattcgcc tgattgcccg cgatgaagcc ctgcacctga ccggtactca
     4861 gcatatgctg aatctgctgc gcagcggcgc ggatgatccg gagatggcgg aaattgccga
     4921 agaatgtaag caggagtgct atgacctgtt tgtccaggcg gcacagcaag agaaagactg
     4981 ggcggattat ctgttccgtg acggttcgat gattggtctg aataaagata ttctctgcca
     5041 gtacgttgaa tacatcacca atatccgcat gcaggcagtc ggtttggatc tgccgttcca
     5101 gacgcgctcc aacccgatcc cgtggatcaa cacctggctg gtgtctgata acgtgcaggt
     5161 tgctccgcag gaagtggaag tcagttctta tctggtcggg cagattgacg cggaagtgga
     5221 caccgacgat ttgagtaact tccagctctg atggcccgcg ttaccctgcg catcactggc
     5281 acacaactgc tgtgccagga tgaacaccct tcccttctgg cggcgctgga atcccacaat
     5341 gttgcggttg agtaccagtg ccgcgaaggt tactgcggct cctgtcgcac ccgactggtc
     5401 gcaggtcagg tggactggat tgccgaaccg ttagccttta ttcagccggg ggaaattttg
     5461 ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga tgtgaattgg ttgtagtgcc
     5521 agatacaacg cttatacgtc ttatctggcc tacaacgatt acatggcgta gtaatacgct
     5581 ttcacctgct cccagtccgc tttggggatt ggctccagat attttccagc tggcggaagt
     5641 catgattaat cgctttattg cgacgcaagc ggcgacgact tttctccagg tcaagaaaac
     5701 cagcttctgc tttaccttct gttttcacat agatatggcg aacataacaa cagccatgct
     5761 gacgattaac gctatgcatt ttcttaaacg ccagcgccac tgctttcaac atggcttgcc
     5821 gtacttcgtc agaatagggc gatactgcat gccgggcata ccagtcagca atgctgatga
     5881 accccgccat atcttcagtc accagcaacg cgcgccattc accctcaatt ttcaccgctt
     5941 caccaaaaac gatcttcggc acgatgacac cggcccgttc cagttcttta attaccgcaa
     6001 cctcacggac aatcgttggt cggccgaacg gataacgtac ggaatgaaac agatgatgcg
     6061 tcatgcgctt tacatacagc tttttgccgt tgcgctcgac gcattgcacc ccgctcatac
     6121 cattacggcg atagttaggc tcttcaaccc agtcgccctc tgttgcccac cagtgattaa
     6181 attcgtcgta ctttgctgaa actgccatac cctatcgcct gtcattttta ttaacgacaa
     6241 tgactatagg tggttacctg aggaaaatct taatgaaacg tgtcgtatta atgaatttct
     6301 tttgccgcta ctagtgtgac accgcttttg cccttcattt gcagcgcata gattccacca
     6361 gccacgacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag gtggatacag
     6421 cgatgaattt tattcgacag ggattaggca tcgccttatt accagagtta acgctgaaaa
     6481 ccattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
//
LOCUS       DAJTVK010000181         6512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000181 DAJTVK010000000
VERSION     DAJTVK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.183"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            38..250
                     /locus_tag="OO372_003925"
     CDS             38..250
                     /locus_tag="OO372_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343296.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="OO372_003926"
     CDS             283..501
                     /locus_tag="OO372_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6343297.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            656..889
                     /locus_tag="OO372_003927"
     CDS             656..889
                     /locus_tag="OO372_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6343298.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="OO372_003928"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="OO372_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6343299.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="OO372_003929"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="OO372_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="OO372_003930"
     CDS             1917..2660
                     /locus_tag="OO372_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6343300.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="OO372_003931"
     CDS             2690..3229
                     /locus_tag="OO372_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6343301.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="OO372_003932"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="OO372_003932"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6343302.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="OO372_003933"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="OO372_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6343303.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="OO372_003934"
     CDS             4582..6102
                     /locus_tag="OO372_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6343304.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6512)
                     /locus_tag="OO372_003935"
     CDS             complement(6135..>6512)
                     /locus_tag="OO372_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343305.1"
                     /translation="ANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKKN
                     TGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQA
                     SKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1712 a         1489 c         1550 g         1761 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gc
//
LOCUS       DAJTVK010000182         6398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000182 DAJTVK010000000
VERSION     DAJTVK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.184"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            21..341
                     /locus_tag="OO372_003936"
     CDS             21..341
                     /locus_tag="OO372_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine transporter"
                     /protein_id="HCR6343306.1"
                     /translation="MTMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASY
                     RYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKD
                     SQRA"
     gene            443..1897
                     /gene="amn"
                     /locus_tag="OO372_003937"
     CDS             443..1897
                     /gene="amn"
                     /locus_tag="OO372_003937"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6343307.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2228..2944
                     /locus_tag="OO372_003938"
     CDS             2228..2944
                     /locus_tag="OO372_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6343308.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3397..3472)
                     /locus_tag="OO372_003939"
     tRNA            complement(3397..3472)
                     /locus_tag="OO372_003939"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3437..3439),aa:Asn,seq:gtt)
     gene            complement(3573..5216)
                     /gene="yeeO"
                     /locus_tag="OO372_003940"
     CDS             complement(3573..5216)
                     /gene="yeeO"
                     /locus_tag="OO372_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR6343309.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            5221..5296
                     /locus_tag="OO372_003941"
     tRNA            5221..5296
                     /locus_tag="OO372_003941"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5254..5256,aa:Asn,seq:gtt)
     gene            complement(5334..6284)
                     /gene="cbl"
                     /locus_tag="OO372_003942"
     CDS             complement(5334..6284)
                     /gene="cbl"
                     /locus_tag="OO372_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6343310.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT         1821 a         1544 c         1373 g         1660 t
ORIGIN      
        1 actatcaata agttagagtc attaccatgg cgcttgaagc acaatctatt ttctggatag
       61 ttttcttctc cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac
      121 cgatgtttac cgaaatgttt ggtgccagtt atcgctatag tggcgctgga gtcggttatc
      181 aggttgccag tgtggttggc ggtggattta caccttttat tgccgctgca ctcatcactt
      241 actttgccgg gaactggcat agcgtcgcca tttatttgct ggctggatgc ctgatttccg
      301 caatgaccgc tttgttgatg aaagacagtc aacgcgcttg atagcctggc gaagatcatc
      361 cgatcttcgc cttacacttt tgtttcacat ttctgtgaca tactatcgga tgtgcggtaa
      421 ttgtatggaa caggagacac acatgaataa taagggctcc ggtctgaccc cagctcaggc
      481 actggataaa ctcgacgcgc tgtatgagca atctgtagtc gcattacgca acgccattgg
      541 caactatatt acaagtggcg aattacctga tgaaaacgcc cgcaaacaag gtctttttgt
      601 ctatccatca ctgaccgtaa cctgggacgg tagcacaacc aatcccccca aaacgcgcgc
      661 atttggtcgt tttactcacg caggcagcta caccaccacg attactcgcc ctactctctt
      721 tcgttcgtat cttaatgaac aacttacgtt gctgtatcag gattatggtg cgcatatctc
      781 tgtgcaaccc tcgcagcatg aaatccctta tccttatgtc atcgatggct ctgaattgac
      841 acttgatcgc tcaatgagcg ctgggttaac tcgctacttc ccgacaacag aactggcgca
      901 aattggcgat gaaactgcag acggcattta tcatccaacg gaattctccc cgctatcgca
      961 ttttgatgcg cgccgcgtcg atttttccct cgcacggttg cgccattata ccggtacgcc
     1021 agttgaacat tttcagccgt tcgtcttgtt taccaactac acacgttatg tggatgaatt
     1081 cgttcgttgg ggatgcagcc agatcctcga tcctgatagt ccttacattg ccctttcttg
     1141 tgctggcggg aactggatca ccgccgaaac cgaagcgcca gaagaagcca tttccgacct
     1201 tgcatggaaa aaacaccaga tgccagcatg gaatttaatt accgccgatg ctcagggtat
     1261 tactctggtg aatattggcg tgggaccatc aaatgctaaa accatctgcg atcatctggc
     1321 agtgctacgc ccggatgtct ggttgatgat tggtcactgt ggcggattac gtgaaagtca
     1381 ggccattggc gattatgtac ttgcacacgc ttatttacgc gatgaccacg ttcttgatgc
     1441 ggttctgccg cccgatattc ctattccgag cattgctgaa gtgcaacgtg cgctttatga
     1501 cgccaccaag ctggtgagcg gcaggcccgg tgaggaagtc aaacagcggc tacgtactgg
     1561 tactgtggta accacagatg acaggaactg ggaattacgt tactcagctt ctgcacttcg
     1621 ttttaactta agccgggccg tagcaattga tatggaaagt gcaaccattg ccgcgcaagg
     1681 atatcgtttc cgcgtgccat acgggacact actgtgtgtt tcagataaac cattacatgg
     1741 tgagattaaa cttcccggtc aggctaaccg tttttatgaa ggcgctattt ccgaacatct
     1801 gcaaattggc attcgggcga tcgatttgct gcgcgcagaa ggcgaccgac tgcattcgcg
     1861 taaattacga acctttaatg agccgccgtt ccgataataa gttctgaacc ttcttatcag
     1921 aaatagatgt gaaggagtaa gtaagacacc tggcaaatag cctgcaataa tcgtgggctg
     1981 tttgcttcct tgggcggata cgagttttat tatcgtctta atgatttcca catattaaaa
     2041 gcaagtatgc tttcaaaaca caattataaa aaatcccgcc aacaatataa gtttttataa
     2101 aattatggct ttagaatatt tttatttcta atagacgaga tttttcctgt tatgatataa
     2161 tatgccgaat taacacatgt taacgattta ccagtaatgt aaataaattt tcgaggagat
     2221 cattccagtg ggacgtaaat gggccaatat tgttgctaaa aaaacggcta aagacggtgc
     2281 aacgtctaaa atttatgcaa aattcggtgt agaaatctat gctgctgcta aacaaggtga
     2341 acccgatcca gaattaaaca catctttaaa attcgttatt gaacgtgcaa agcaggcaca
     2401 agttccaaag cacgttattg ataaagcaat tgataaagcc aaaggcggcg gagatgaaac
     2461 gttcgtgcag ggacgttatg aaggctttgg tcctaatggc tcaatgatta tcgccgagac
     2521 attgacttca aatgttaacc gtacgattgc taacgttcgc acaattttca ataaaaaagg
     2581 cggcaatatc ggagcggcag gttctgtcag ctatatgttt gacaatgctg gtgtgattgt
     2641 atttaaagga tcagaccctg accatatttt tgaaatttta cttgaagctg aagttgatgt
     2701 tcgtgatgtg actgaagaag aaggtaacat tgttatttat accgaaccta ctgaccttca
     2761 taaaggaatc gcggctctaa aagcagctgg aatcactgag ttctcaacaa cagaattaga
     2821 aatgattgct caatctgaag ttgagctttc cccagaagat ttagaaatct ttgaagggct
     2881 tgttgatgcc cttgaagatg acgacgatgt acaaaaagtt tatcataacg tcgcaaatct
     2941 ctaattatct tttaaagaaa tctgtcttta cggcagattt ctttaatctc atataattct
     3001 tataaaaaat ataagattca actcgtcata ttgattatac ccccccgttc ccagagaaat
     3061 aatatttatt aaaattccag ttcttctttt tctgattaca gaaggcaaag tggcaattac
     3121 gcatagtttc ccgataaaga cgcgatagcg acatcccgca taaggcattt ttctctttat
     3181 ctttgtacgg tacttcatgg aacagagttt ttgaccttgc gaatcgtgat gtctgttggg
     3241 gaggggcaat ttgctcactg aagcgtgaga ctcgattaag cgcacgaaac acagaaatca
     3301 aaaaacccgg tcactttttt acaaggtaac cgggtaaaaa taatttttat tttttaactg
     3361 ttttgagact catagagatg tctcaaaact aaaatttggc tcctctgact ggactcgaac
     3421 cagtgacata cggattaaca gtccgccgtt ctaccgactg aactacagag gaatcgtgag
     3481 aacgaggcga atattagcga tgcccaccca caatgtcaaa gcctgttttt taaatttgaa
     3541 atcgtttgct gaaataatct gcattttgtc gtttattccg acacaactgg ctttttttca
     3601 cacttttgcg gttcggatcg agggtatttc cacagccaac gtccagtaac cattcgccag
     3661 taaaacagca cggcccgcac agcccagtcg gcaaacattc ccatccagac accaaccaca
     3721 ccccagccaa gcatgatgcc cagcacataa ccgacgacaa cccggcaacc ccacatgctc
     3781 aacatagaaa cccacatggc gtaacgggca tcgcgagcac ctttaaatcc agcgggtagc
     3841 acccatgagg cggaccaaat aggcataaat aaagcattta gccaaatcag aatcacaacg
     3901 acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca taaccccggc aaagggagcc
     3961 gttagccagg cgatggccgt taatccaaga gtggaaagcc agaacacatg ccgcaactga
     4021 atctctgctt gcgctatctg ccctaccccc aaccttcggc ctgtaatgat cgtagaagca
     4081 gagccgagcg cacttccggg taagttgata agagccgcaa ttgaaaacgc gataaaattt
     4141 ccggcaataa cactggtccc catcccggca acgaacattt gggttaataa ccgaccactg
     4201 gtaaataaca ctgattcgac actcgcggga ataccaatcc ccatgacttc ccagataatg
     4261 ctaaaattca gcggtttaaa atagctcttt aacgaaatcc ttagcgcagg attaaaacca
     4321 atcgccagca cccacaaaat tgcaactgcg ccaatataac gagaaatggt taaacccagc
     4381 cctgccccga caaatcccag tcccggccag gagaaaaggc cgtaaatcaa tatgccgcta
     4441 ataataatat taagaatatt caggctaccg ttaatcaata gcggtatttt cgtattccct
     4501 gcaccacgaa gtgccccgct accaataaga gtgatggcag ctgctggata actgagtacc
     4561 gtcagctcca gataagtcaa cgccagtgct ttaacttctg tcgtggcatc acccgcgacg
     4621 aaatcaataa tttgttcgcc aaaatgatga ataagcgttg ccaacagtac ggcaaacaac
     4681 gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc gtcgtcgatc ccgcttaccg
     4741 agactaaatg ccacaacgac agtagtacca agatcgatag cagcaaaaaa agccataatg
     4801 accatattga agctgtccgc caatcccacg ccggccatcg catcttttcc cagccagctg
     4861 accagaaaag tgctcagaac ccccatcaac aggacacagg cattctccat gaagatagga
     4921 acagcaagcg gggttatctc gcgccagaac aacactttgt agctcttgcg tttagcgtgc
     4981 cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga tattcaaagc cgaccttaat
     5041 tgcagaaagt gaaaccacat ttcaaataat gagggagaat cagcaaagct gcaaagattt
     5101 tcgccaacaa attgtctgca aatgcaacaa actgttgata gaaacggcaa acagttgggg
     5161 aatttaaaaa tcgggtttga caaaagattt ttcgccgtta agatgtgcct caacagcgat
     5221 tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac tggttcgagt
     5281 ccagtcagag gagccaaatt tctgaaaagc ccgcttttat agcgggcttt ttgctatatc
     5341 tgataatcaa tttcctcttc actgttttcc atcacctgcc gcttgatatc ctcaactgac
     5401 agtcctgcat tacaaagttc cagaaagcgc cagacatagt tacgctgaag ttgtcctcgc
     5461 ttcagtccca accagacagt attagcatcg aaaagatgcc gcgtatccag acggattaaa
     5521 ttctcttcct cttgttcgcc gctggattgt tcggcaacta atccgatccc aagcccaaga
     5581 gcaacatagg ttttaatgac atcagaatcc tgcgcactta atacaatatc tgccagcaaa
     5641 cctttgcggg caaatgcgtc atcaatacgt gagcgccccg taatcccctg tcggtaagtg
     5701 attaacggcc acttcgctat tgattccagc gtcaatggtg taatttgcgt taagggatga
     5761 tcgagtggaa caagcaaact atggtgccaa cgaaaccacg ggaaggcgac gagctgcggg
     5821 tcattactca aacgctcgct ggcgatacca atatcagctt cgccattttg caacaacgtc
     5881 gcaatttcct gtggcgtccc ctggattagc tcgagccgaa cctccgggaa aagttcgcga
     5941 aaagctttaa tgacctctgg caagctataa cgtgcctgag tatgcgtcgt tgcaatagtg
     6001 agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc gacgaacatt actggcttca
     6061 ttcagaatac gttctgcaat gaccagtaat gctttgcccg gttcagtcat gcccagcagt
     6121 cgcttacctc gtcgaacaaa tatttcgatg ccaagttcat cctccagttc ccgaatatga
     6181 cggctgacgc cggactgtga ggtaaaaagc atattcgcaa cctctgtcag gttgtaatcc
     6241 tgacgtgcag cctcgcggat tatctttagt tgttggaaat tcacggtaaa ctccgggcag
     6301 ttcagatttc ccgttattgt taaagtctaa tgcccggcat aacaaataat aaaaacccgc
     6361 atcttattcc atcccgatat aacgcttagc tcaccaat
//
LOCUS       DAJTVK010000183         6253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000183 DAJTVK010000000
VERSION     DAJTVK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.185"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            122..952
                     /locus_tag="OO372_003943"
     CDS             122..952
                     /locus_tag="OO372_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HCR6343311.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO372_003944"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="OO372_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6343312.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="OO372_003945"
     CDS             complement(2081..3541)
                     /locus_tag="OO372_003945"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6343313.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OO372_003946"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="OO372_003946"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6343314.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6253
                     /gene="gntP"
                     /locus_tag="OO372_003947"
     CDS             5146..>6253
                     /gene="gntP"
                     /locus_tag="OO372_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6343315.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIIS"
BASE COUNT         1480 a         1618 c         1587 g         1568 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtg
//
LOCUS       DAJTVK010000184         6203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000184 DAJTVK010000000
VERSION     DAJTVK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.186"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            12..754
                     /locus_tag="OO372_003948"
                     /pseudo
     CDS             12..754
                     /locus_tag="OO372_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="OO372_003949"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="OO372_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="OO372_003950"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="OO372_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="OO372_003951"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="OO372_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6343316.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="OO372_003952"
     tRNA            complement(2777..2851)
                     /locus_tag="OO372_003952"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="OO372_003953"
     CDS             complement(2927..3859)
                     /locus_tag="OO372_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR6343317.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="OO372_003954"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="OO372_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6343318.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(5090..5425)
                     /locus_tag="OO372_003955"
     CDS             complement(5090..5425)
                     /locus_tag="OO372_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343319.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDNVEKRLQNRQAKYQNEINQLS
                     APGVNFDDI"
     gene            complement(5383..5775)
                     /locus_tag="OO372_003956"
     CDS             complement(5383..5775)
                     /locus_tag="OO372_003956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343320.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            complement(5782..6158)
                     /locus_tag="OO372_003957"
                     /pseudo
     CDS             complement(5782..6158)
                     /locus_tag="OO372_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1546 a         1369 c         1374 g         1914 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactgatgtt cgtcactgac
     5041 tatttccgat attcaggcgt tgaaaaatca aaaaactgga aaaataattt taaatatcat
     5101 caaaattaac acctggcgca gatagttgat taatttcatt ttgatatttc gcctggcgat
     5161 tttgaagtct tttttcaacg ttatcttcga gatccttaaa gtctttgtat ttattttcag
     5221 gatggtcaac gtcaactttg ttagcgaata aattagaaat tatttctttt acattgtcgc
     5281 gtctaaattt tgcatcgtcc acttgaaaat tatatcttat tgctcgacct aaacgtttaa
     5341 aaacttcgtt aggttctctc agtctttccc cgttgagctc gtttacaacc tgatgatcgg
     5401 aacctgttgc aaatgattct gccatgatag ctttactgct ttcaaaccac gctgaagcat
     5461 tgctgaagca ttcctggctt tttcactaca atattgggtg agactatcac cattaatacg
     5521 aattcctctt tgaatgtggt agtcgtcctg atcgtagtaa ccgagagata agtaatcatt
     5581 tccgttttta tttttaatta caatagcgag agagttgcct tttttatctt taccgtacca
     5641 ggtaaataag tccgttggtt cgctattccc gctctcatca aattctgtta ccgtatgtct
     5701 gagtttgtgt gatatcttat cgaagataaa accaggatct ccatcatata tttcttttaa
     5761 tgcgctgaag atcatttcct tgttattgat atcatgagtc ttctcaacta tgttgtcatt
     5821 aatgatagta cggacgagtt ctttgtcttt atagttcgca ggtaattcta ttggttcttg
     5881 gttgtcgata ccaatagtaa tatggtctgt atcattggag ccaatatcaa tataagtaac
     5941 tgaggaatta ttaatattaa atcttaagga tcgtcgttta ctcgtatggc gttttttata
     6001 agctcaagta gatgtttaat acaggtgctt attccttcct taccattaaa aaggtttttg
     6061 agctcttgta ctaatccatt aacttcatca ttgttatttt cattaatgga ttctgggtaa
     6121 gttgcgctgg tattgttgat tgaaatagat ggtagcatga ttttattcca ttttgattga
     6181 tttgttacaa atatatatta cct
//
LOCUS       DAJTVK010000185         6130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000185 DAJTVK010000000
VERSION     DAJTVK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.187"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..225
                     /locus_tag="OO372_003958"
     CDS             <1..225
                     /locus_tag="OO372_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6343321.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            271..621
                     /gene="traA"
                     /locus_tag="OO372_003959"
                     /pseudo
     CDS             271..621
                     /gene="traA"
                     /locus_tag="OO372_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            636..947
                     /gene="traL"
                     /locus_tag="OO372_003960"
     CDS             636..947
                     /gene="traL"
                     /locus_tag="OO372_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCR6343322.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            969..1151
                     /locus_tag="OO372_003961"
                     /pseudo
     CDS             969..1151
                     /locus_tag="OO372_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1153..2403
                     /locus_tag="OO372_003962"
                     /pseudo
     CDS             1153..2403
                     /locus_tag="OO372_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2400..2786
                     /gene="trbI"
                     /locus_tag="OO372_003963"
     CDS             2400..2786
                     /gene="trbI"
                     /locus_tag="OO372_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCR6343323.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2783..3094
                     /locus_tag="OO372_003964"
                     /pseudo
     CDS             2783..3094
                     /locus_tag="OO372_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            3089..4087
                     /locus_tag="OO372_003965"
                     /pseudo
     CDS             3089..4087
                     /locus_tag="OO372_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            4107..4854
                     /gene="traX"
                     /locus_tag="OO372_003966"
                     /pseudo
     CDS             4107..4854
                     /gene="traX"
                     /locus_tag="OO372_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4909..5469
                     /locus_tag="OO372_003967"
     CDS             4909..5469
                     /locus_tag="OO372_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6343324.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5599..5811
                     /locus_tag="OO372_003968"
     CDS             5599..5811
                     /locus_tag="OO372_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6343325.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1462 a         1484 c         1865 g         1319 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcagcg ttaagtgttc agggtacttc tgcgcccgtc
      301 aaaaagaaat cgtttttttc taaattcact cgtctgaata tgcttcgcct ggttcgtgca
      361 gtgatcccgg ttgctgttct gatgatgttg ttccctgagt tagctatggc tgctggtaaa
      421 ggcacggacc tgatggctaa aggtcaggaa acggttaagg cgaccttcgg taaggactcc
      481 agtatcgtta aatgggttgt tctggctgaa gttctggtcg gtgcagtcat gtacatgatg
      541 accaaaaacg tcaagttcct ggtcggcttt gccatactct ctgtatttat tgcagtaggt
      601 atgtctgttg ccggattctg acaggaaata aaacgatgtt gggagacgag aataaactta
      661 agaaatatcg tttcccggaa acactgacca accagagccg ctggtttggc ctgccactgg
      721 atgaactgat ccccgcagca atctgtgttg gctggggtat cacaacatcg aaatatctgt
      781 tcggtattgg tgcagcggtt ctggtttatt tcgggattaa aaaactgaaa aaagggcggg
      841 gcagttcctg gttacgtgac ctgatttact ggtatatgcc aacagccctg ctgcgcggta
      901 tttttcataa tgttcccgat tcgtgtttcc ggcagtggat taaatagaac tgataccagg
      961 attgttatat ggaacacggt gcccgtttaa gtaccagtcg tgtaatggcc atcgccttta
     1021 tatttatgtc agtgcttatt gttctcagcc tctctgttaa cctcattcag ggggtgaata
     1081 actaccgtct tcagaatgag caacgcactg ccgtgacccc aatgggattt aatgtgccct
     1141 ttgccgtgtc acacctaccg taaccagctg gcgtttatcg atattttctt ccggggaatg
     1201 aataacacca actacaacat ggcggtctgt ggcacctccg gggccggtaa aaccgggctg
     1261 atacagccac ttatccgcag cgtgctggac tccgggggct ttgccgtggt gttcgacatg
     1321 ggggatggct acaagtccct gtgtgagaac atgggtggcg tatatctgga cggtgaaacc
     1381 ctgcgtttta acccgtttgc gaacatcacc gatatcgacc agtcagcgga gcgtgtccgt
     1441 gaccagttgt cagtgatggc cagccccaac ggtaacctgg atgaagtgca tgaaggtctg
     1501 ctgctgcagg cggtcagggc ctcctggctg gccaaagaga acagggcacg tattgatgac
     1561 gtggtggatt tcctgaaaaa cgccagtgac agcgagcagt atgccgggtc accgactatc
     1621 cgcagccgtc tggacgaaat gattgtgctg cttgaccagt acactgccaa cggcacttac
     1681 ggccagtatt ttaactctga tgagccgtcc ctgcgggatg acgcaaaaat ggtggtgctg
     1741 gagctgggcg gactggaaga ccgtccgtca ctgctggttg cggtgatgtt ctccctgatt
     1801 atctacatcg agaacaggat gtaccgcacg ccgcgtaacc tcaagaaact gaacgtcatt
     1861 gatgaaggct ggcgtctgct ggacttcaaa aatcacaagg tcggtgagtt tattgagaaa
     1921 ggttaccgta ccgcccgtcg tcataccggt gcttatatca ccatcacaca gaacatcgtc
     1981 gactttgact ctgacaaggc ctccagtgcg gcccgtgcgg catggggtaa ctcctcctac
     2041 aaaattatcc tcaaacagag tgcgaaggag ttcgcaaaat acaaccagct gtatccggac
     2101 cagttccagc cactgcagcg cgacatgatt ggtaagtttg gtgcggccaa agaccagtgg
     2161 ttcagttcct tcctgctgca ggtggaaaac cattcctcct ggcaccgcct gtttgtggac
     2221 ccgttaagcc gcgccatgta cagctctgac ggcccggatt ttgagtttgt gcagcagaaa
     2281 cgtaaagagg ggctgagtat tcatgaggcg gtgtggcagc tggcgtggaa gaagtcaggg
     2341 ccggagatgg cttcgctgga agcctggctg gaagaacatg agaaatacag gagtgttgca
     2401 tgaccacaac gcaaaaaacg actgatgtta cagccccccg acgcagccac tggtggtgga
     2461 cggtgccggg atgcctggca atggtgttac tgaacgcagc cgtcagttac ggcattgtca
     2521 gactgaacgc accgatgact gccgccttca acatgaaaca gactgtggat gcattttttg
     2581 acagcgccag tcagaaacaa ctgtcagaag cacagtcaaa agccctttca gcgcgtttta
     2641 acacggcact ggaagccagc ctgcaggcgt ggcagcagaa acaccacgcg gtcattctgg
     2701 tgtcgcctgc cgtggtacag ggggctccgg atatcacccg ggaaatccag caggatattg
     2761 cccggcgaat gagagcggaa ccatgagatg ccgggggctg attgccctgc tgatatgggg
     2821 gcagagtgtg gccgccgccg atcttggcac ctggggtgat ctgtggccgg taaaggagcc
     2881 ggacatgctg acggtgatca tgcagcgcct gacggcactg gagcagtccg gtgagatggg
     2941 ccggaaaatg gatgccttta aggagcgggt gatccgcaac agtctgcggc cccctgccgt
     3001 gcccggtatc ggacgcacgg agaaatacag cagccggttg tttgacccgt ccgtcagact
     3061 ggctgcggat atccgggata acgaagggcg ggtggccggc tttgagtcag cctacgtggc
     3121 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     3181 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     3241 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     3301 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     3361 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     3421 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     3481 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     3541 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     3601 ccgtgataat cctgacagtg gtgtggtggt aagcattgcc ggtgaaggcc gtccgtggaa
     3661 tcccggtgcc atcaccgatg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     3721 gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     3781 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     3841 caaacctgac ctgccggacg gcaaaacaga gctggctgtc agggaaattg ccgggcagga
     3901 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tgctgcgtga
     3961 atcacaacgg gagcgggaag cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg
     4021 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     4081 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     4141 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     4201 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     4261 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     4321 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     4381 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccctggt
     4441 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     4501 cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat ggccctgttg ggggcctttg
     4561 tccggcacca gttacggcat tgccgggctg ctgatgctgg cagtcagcca ccgactgtac
     4621 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     4681 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     4741 accgttggtc tggtgtcgtg tgcaggaaaa tcattacccc gtttctggcc cggggatttt
     4801 ttcccggtat tctacgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     4861 tggtatcttt gaccgtaaca ggatgattcg tcagaggcag aacacagcat gacagagcag
     4921 aagcgaccgg tactgacact gaagcggaaa acggaagggg agacgcctgt ccgcagccgg
     4981 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     5041 gagaaagcag cccgggaagc agagctggcg gcaaaaaaag cgcaggccag acaggcactg
     5101 tccatttatc tgaacctgcc cacgctggat gaggccgtga ataccctgaa accctggtgg
     5161 ccgggattat ttgacggtga caccccccgg cttctggcct gcggtatccg ggacgtgtta
     5221 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     5281 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     5341 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aagaagcgta tgcggcagcg
     5401 cgtctggata aaatccgccg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     5461 gagaaataaa aaaaagcctc catcagagag gaggcaggga aataaggctt aaaaggaaat
     5521 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     5581 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     5641 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     5701 gcggcagaag catggcgacg ggctgcctgt gttgcgccca ggacagactg gcagcagttt
     5761 gcccgaaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     5821 cagccttcat tactccagga gtcagaaggc tgaaaggagt ttatgagatt tgcttttttt
     5881 aatatccggt cgggcagtat gaccggacag ttctgagtag cacagagttt tttccgtatt
     5941 aagtcgtgat tttatatttt gtgattaatt tcacaaaata aggtgttatt cagtgtgtgc
     6001 tgcaatattc aggatgcctg aaatacagat gctatttatc tgatatggag aataacatga
     6061 ggaaatagta aggcagtatg actttccgtg agacagatgc tggccaacgt gggatattta
     6121 acgcgggtta
//
LOCUS       DAJTVK010000186         6070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000186 DAJTVK010000000
VERSION     DAJTVK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.188"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..855
                     /locus_tag="OO372_003969"
     CDS             <1..855
                     /locus_tag="OO372_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6343326.1"
                     /translation="FGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPG
                     TKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAET
                     IGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVG
                     GIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEH
                     LELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQ
                     DTPDMREY"
     gene            923..4465
                     /locus_tag="OO372_003970"
                     /pseudo
     CDS             923..4465
                     /locus_tag="OO372_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            5446..6070
                     /locus_tag="OO372_003971"
                     /pseudo
     CDS             5446..6070
                     /locus_tag="OO372_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         1532 a         1458 c         1870 g         1210 t
ORIGIN      
        1 tttggcaacc tggcgatacc gcgtgtgggt caggaggtga ttgtggactt cctcaacggc
       61 gacccggacc agccgattat tatggggcgt acctaccacc aggaaaaccg cacacccggc
      121 agcctgccgg ggacgaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
      181 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcag
      241 aagaacatga acaccgaggt gctgaataac cgcaccacgg atgtgataaa caaccatgct
      301 gaaaccattg gcaacaatca gatgattgcg gttaccaaca atcagataga gacggtgggc
      361 gttaaccaga tagagacggt gggcagtaac cagataatta aagtgggttc tgttcaggtg
      421 gaaacgattg gacttgttcg tgcgctgacg gtgggcgtgg cgtatcagac gacggtaggt
      481 ggcattatga acacctcggt ggcactgatg cagtcctcgc agatcggttt gcataaatcg
      541 ttgagggtcg ggctgggtta tgacgtcaaa gtcggaaata acgttacctt caccgttggt
      601 aaaacgaaaa aggatgatac cgggcagacc gcgatttact ccgccggtga gcatctggag
      661 ctctgctgtg gtaaggcaag gctggtgctg acgaaggacg gacaaatttt tctcaacggc
      721 acaaaaattc atttgcaggg taaggagcag gttaatggtg actcactgtt gattaactgg
      781 aactgtgcag cctcgaaatc cccaccgaag acccctgatg aaaagcagga tacgccggat
      841 atgagagagt actgatcccg tacctgttcg gtattcagta acaaaccagc attaaaaata
      901 aacagaagaa ggaatttgaa tcatgagcgg aaaaccagcg gcgcgtcagg gagatatgac
      961 tcagtatggc ggtcccattg tccagggttc ggcaggtgta agaattggcg cgcccaccgg
     1021 cgtggcgtgc tcggtgtgtc cgggcgggat gacttcgggc aacccggtaa atccgctgct
     1081 gggggcgaag gtgctgcccg gcgagacgga ccttgcgctg cccggcccgc tgccgttcat
     1141 tctctcccgc acctacagca gctaccggac gaagacgcct gcaccggtgg gcgttttcgg
     1201 ccccggctgg aaagcgcctt ctgatatccg cttacagcta cgtgatgacg gactgatact
     1261 caacgacaac ggcgggcgga gcattcactt tgagccgctg ctgccggggg aggcggtgta
     1321 cagccgcagt gagtcaatgt ggctggtgcg cggtggtaag gcagcacagc cggacggcca
     1381 tacgctggcg cggctgtggg gggcgctgcg ccggatatcc ggttaagccc gcatctttac
     1441 ctggcgacca acagcgcaca ggggccgtgg tggatactgg ggggggctga gcgggtgccg
     1501 ggtgctgagg acgtactgcc agcgccgctg ccgccgtacc gggtgcttac cgggatggcg
     1561 gacgctgacg taccggcgtg aggccgccgg tgacctggcc ggggaaatca ccgacgtgac
     1621 ggacggtgcc gggcgggagt tccgtctggt gctgaccacg caggcgcagc gtgcggaaga
     1681 ggcccgcacc tcttcgctat cttcttctga cagttcccgc cctctctcag cctcagcgtt
     1741 ccccgacaca ctgcccggta ccgaatacgg ccccgacagg ggtatccgcc tttcggcggt
     1801 gtggttgatg cacgacccgg catacccgga gagcctgccc gctgcgccac tggtgcggta
     1861 cacgtatacg gaagccggtg aactgctggc ggtatatgac cgcagcaata cgcaggtgcg
     1921 cgctttcacg tatgacgcgc agcacccggg ccggatggtg gcgcaccgtt acgcgggaag
     1981 gccggagatg cgctaccgct acgacgatac ggggcgggtg gtggagcaac tgaacccggc
     2041 agggttaagc taccgctatc tttatgagca ggaccgcatc accgtcaccg acagcctgaa
     2101 ccggcgtgag gtgctgcata cagaaggcgg ggccgggctg aaacgggtgg tgaaaaaaga
     2161 actggcggac ggcagcgtca cgcgcagcgg gtatgacgcg gcaggaaggc tcacggcgca
     2221 gacggacgcg gcgggacggc ggacagagta cggtctgaat gtggtgtccg gcgatatcac
     2281 ggacatcacc acaccggacg ggcgggagac gaaattttac tataacgacg ggaaccagct
     2341 gacggcggtg gtgtacccgg acgggctgga gagccgccgg gaatatgatg aaccgggcag
     2401 gctggtatcg gagacatcgc gcagcgggga gacagtacgc taccgctacg atgacgcgca
     2461 cagtgagtta ccggcgacga caacggatgc gacgggcagc acccggcaga tgacgtggag
     2521 ccgctacggg cagttgctgg cgttcactga ctgctcgggc taccagaccc gctatgaata
     2581 cgaccgcttc ggccagatga cggcggttca ccgcgaggaa ggtatcagcc tttaccgcca
     2641 ctatgacaac cgtggccggt taacctcggt gaaagacgca cagggccgtg aaacgcagta
     2701 tgagtacaac gccgcaggcg acctgactgc cgttatcacc ccggacggca accggagcga
     2761 gacacagtac gatgcgtggg gaaaggcggt cagcaccacg cagggcgggc tgacgcgcag
     2821 tatggagtac gatgctgccg gacgtgtcat cagtctgact aacgagaacg gcagtcacag
     2881 cgacttcagc tacgatgcgc tggaccggct ggtacagcag ggcggctttg acgggcggac
     2941 gcaacgttat cattatgacc tgaccggaaa actcacgcag agtgaggatg aggggcttgt
     3001 caccctctgg cactacgatg cgtcggaccg catcacgcac cggacggtga acggcgaccc
     3061 ggcagagcag tggcagtatg acggccacgg ctggctgaga gaaatcagcc acctgagcga
     3121 aggccaccgt gtcgctgtcc actacggcta cgacgataag ggccgcctga cgggcgaacg
     3181 gcagacggtg gagaacccgg agacggggga actgctgtgg cagcattaga cgaaacacgc
     3241 ctacaacgaa caggggctgg caaaccgcgt cacgccggac agcctgccgc cggtggagtg
     3301 gctgacctac ggcagcggtt acctggcggg catgaagctg ggcgggacgc cgctggtcga
     3361 gtatacgcgg gacaggctgc accgtgagac ggtgcgcacc ctcgcaccgg acctggacat
     3421 ccgcatcccg tatgccaccg acccggcggg aaaccgcctg ccggacccgg agctacaccc
     3481 ggacagcacg ctcacggcgt ggccggataa ccgcatcgca gaggatgcgc actatgttta
     3541 ccactatgat gaatacggca gactgacgga gaagacggac cgcatcccga cgggtgtgat
     3601 acggacggac gacgagcgga cccaccacta cgacagccag caccgcctgg tgttccacac
     3661 gcggatacag catggcgagc cactggtcga gagccgctac ctctacgacc cgctgggacg
     3721 gcgaatggcg aaacgggtat ggcggcggga gcgtgacctg acggggtgga tgtcgctgtc
     3781 gcgtaaaccg gaggagacat ggtacggctg ggacggcgac aggctgacga cggtacagac
     3841 cgacaccaca cgtatccaga cggtatacga gccgggaagc ttcacgccgc tcatccgggt
     3901 cgagacagag aacggcgagc gggagaaagc gcagcgccgc agcctggcgg agaagctgca
     3961 gcaggaaggg agcgaggacg ggcacggcgt ggtatttccg gctgaactgg tgcggctgct
     4021 ggacagactg gaggaagaaa tccgggcaga ccgcgtgagc agtgaaagcc gggtgtggct
     4081 tgcgcagtgc gggctgacgg tggagcaact ggccagacag gtggagccgg aatacacacc
     4141 agtgcgaaac gttcatcttt accactgcga ccatcggggc cagccgctgg cgctcatcag
     4201 cgaagacggc gatacggtgt ggagcggggc gtatgatgag tggggcaacc agctaaatga
     4261 ggagaacccg catcatgtgt atcagccata ccgtctgccg gggcagcagt atgatgagga
     4321 gtcggggctg tactacaatc gtcaccggta ctatgatccg ttgcagagac ggtacatcac
     4381 ccaggacccc attgggctga aaggtgggtg gaatctgtat cagtatccgc tgaatccggt
     4441 gagcaggata gatccgttgg ggttattctc atgtgagagt tgtagtcatt ttatagatga
     4501 tataaataat aaactggata accttccgtt taacccgaca aaaatgttta cagctagttt
     4561 aaatactgct aattcagcaa ggctgtatgc aagtggcatg acaaaattcg gtataagtgt
     4621 tgctgccgat ggaacgatca ttgctgcccc agtcggtgca gcttctatgg ctttaggggc
     4681 gtggaatata aaatctgcga cagctgcata taacagagca aatttacaat tatcagaatc
     4741 acttcaggaa aatggtagag ataggtgttg gagaaattta ttgggtattt taccttatgg
     4801 tgagcagttt gatgaccctg gtgaaccatc aatattagac gtttatggtg aaaagattga
     4861 gaatatcaaa gattctccaa tgaagttttt tgaagagctt gggacgctgg ggttgtaatg
     4921 tgtattttta atgatatttt tatgtttgtg tttattgtta gtagtatact tgtaaccgtg
     4981 cattttgtgt attctttatt ttttaaaagt ggaaaggtaa aagataaggt aaaaaaatgg
     5041 attaaagtaa tgtgtgattt attttggggg ataggatagt ggtgtgatat ggataaatat
     5101 aaattacagt ggctggcata tttcgacctg acgccggagt agataaaaaa acagacagga
     5161 ttggagtata gcaagcgtct gacaatctct cgggacagta gtatgatgaa gaggcggggt
     5221 tgtactataa ccgtaaccgg tactacgatc cgctaaaggg caagtatatg actcagggaa
     5281 agatcatcag aagttcactt tttgtactaa ataattcgca ttttatgttt aaaaattgag
     5341 atattcctca ttacctgaag ctgttttttt attgcttata cataatcaaa tactccttac
     5401 ataattaagg agaacaaaat ggaaattaaa aaattgatgg aacatttttt gaagcaatat
     5461 ggtgattttg aaaatggcat tcctgttcac gataccattg ccagagttgt atcctgtatc
     5521 agtcctgcaa aatttcatga gtgctttatt aactggatgc gtgactgcca ttcttcagat
     5581 gataaagacg tcattgcaat tgatggaaaa acgctccggc actcttatga taagagtcgt
     5641 cgcaggggag cgattcatgt cattagtgcg ttctcaacaa tgtacagtct ggtcctcggg
     5701 cagatcaaga cggatgagaa atccaatgag attacagcca ttcctgaact tcttaacatg
     5761 ctggatatta aaggaaaaat catcacaact gatgcgatgg gttgccagaa agatattgca
     5821 gagaagatac aaaaacaggg aggtgattat ttattcgctg taaaaggaaa ccaggggcgg
     5881 ctaaataaag cctttgagga aaaatttccg ctgaaagaat taaataatcc agagcatgac
     5941 agttacgcaa tcagtgaaaa gagtcacggc agagaagaaa tccgtcttca tattgtttgc
     6001 gatgtccctg atgaacttat tgatttcacg tttgaatgga aaggactgaa gaaattatgc
     6061 gtggcagtct
//
LOCUS       DAJTVK010000187         6038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000187 DAJTVK010000000
VERSION     DAJTVK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.189"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..276
                     /locus_tag="OO372_003972"
     CDS             <1..276
                     /locus_tag="OO372_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6343327.1"
                     /translation="EYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQV
                     CGYTVNADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(316..987)
                     /locus_tag="OO372_003973"
     CDS             complement(316..987)
                     /locus_tag="OO372_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR6343328.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1094..1327)
                     /gene="yedF"
                     /locus_tag="OO372_003974"
     CDS             complement(1094..1327)
                     /gene="yedF"
                     /locus_tag="OO372_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6343329.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1324..2529)
                     /gene="yedE"
                     /locus_tag="OO372_003975"
     CDS             complement(1324..2529)
                     /gene="yedE"
                     /locus_tag="OO372_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6343330.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2716..3129
                     /locus_tag="OO372_003976"
     CDS             2716..3129
                     /locus_tag="OO372_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6343331.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3163..4650)
                     /gene="amyA"
                     /locus_tag="OO372_003977"
     CDS             complement(3163..4650)
                     /gene="amyA"
                     /locus_tag="OO372_003977"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6343332.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4728..5093)
                     /gene="fliT"
                     /locus_tag="OO372_003978"
     CDS             complement(4728..5093)
                     /gene="fliT"
                     /locus_tag="OO372_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6343333.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5093..5503)
                     /gene="fliS"
                     /locus_tag="OO372_003979"
     CDS             complement(5093..5503)
                     /gene="fliS"
                     /locus_tag="OO372_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6343334.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5518..>6038)
                     /gene="fliD"
                     /locus_tag="OO372_003980"
     CDS             complement(5518..>6038)
                     /gene="fliD"
                     /locus_tag="OO372_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6343335.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1478 a         1588 c         1501 g         1471 t
ORIGIN      
        1 gagtacaagc agctctggcg tggcggtcag gtgcttgctg ttccgccagc gtacacaagc
       61 cagcgttgcg cgtgctgtgg tcatacagcg aaagaaaacc gcctgtcaca aagtaaattc
      121 agatgccagg tatgtggata tacagtgaac gccgatgtaa atggcgctcg taacatttta
      181 gcggcggggc acgccgttct tgcctgtgga gagatggtgc agtcaggccg cccgttgaag
      241 caggaaccca ccgaaatgat tcaggcgaca gcctgaacgt agcagggatc ctcgtccttc
      301 agggcgtgga ggatgtcaac aaacaggttg aattaactcc gcgccctggt ttttgacatt
      361 acctaccgcg cgcgataccg ggtgccaggt gaactggttt gctggcacac agccgttggt
      421 agcaatttct gatgcttctt taccgccaat ctcttgccgc atccattcct gtgcagcttc
      481 tggcgacaga accagcgggc ggcggtcatg aatgtctacc agaccctggt ctgctgcagc
      541 agtgacaatt aaaaatcctt cggcttcgtc accacgctca aatggtgtgc tacctatcgc
      601 ggccataaaa ataggttgtc catcagcacg atagataaaa aaaggctgtt ttttgtcgcc
      661 ttcttttttc cactcaaacc agccatcggc aaaacagatt gcccgaccat gttgccagag
      721 cggtttaaac atgcgactgg ttgcggcagt ttctacgcgg gcgttaatca gcggcggttt
      781 atcccaccat ccgggagcat atccccagaa aaccggatcc agatgaaggt gttcatcacg
      841 ttcactgagc agaagaactt tggttcccgg cgcgacgttg tatctgccaa tgggttcggg
      901 atcgtaggga atatcgcgtt caatatcttc cgcgagaagc gcaaggtaat cttcacgcgt
      961 ttgggattgg gcaaagcgtc cacacataga aacctcctga gatcatactg aaaagaatag
     1021 gagagggggc gggtaaaatc tatgtgtcag aggcaacaat gttcccgctg tgggccacag
     1081 cgggaatgag ggattacttc tgaattaaat aacgaatggt cggcccgtct tgctgaatat
     1141 ccagcaccgt atagccgtga ttacgcgcat ccagtggaat attattgatc gactgtggac
     1201 agtcgctcac cacttccagg atttcccctt tttttaactg cggcatcgcc tcaagggttg
     1261 cgactgccgg ataagggcag ggttcaccca ccatatcaag gcggtaatca ggaacgatat
     1321 ttttcatgcg atctccttag cagtctgcgg tgccgcacgg cggaagaagc gtttttccca
     1381 gccgataatc aacatcagcg cagtaaacaa cagcaaatat gtcaccagca ggccacccat
     1441 cgggccaaag gttttcagca ggttgatttt gtcccagtcg gtggccagcg ccggagcgaa
     1501 atcatcccag taatacgcca gaatcgtcga gccgatcaca ttgcccagac cgacccacca
     1561 gtagtgcacc tggccttcta ccgcgcggta catccagccg gtttcgcagc cgcctgccag
     1621 cacgatgcca aaaccaaaca gtaaaccacc aattaccgcg tttggtcccg cccacatgat
     1681 tttgggttca acgcctaact gtacgtaact gaagatcccg atggcactca ccgccatacc
     1741 gataatgatt gctttcgcca tatgggtacg tccggtgatc cacatatcgc ggaacgccga
     1801 agtaaagcag atttgcgcac gttcaatcag taaaccaaag ccgacgccaa acagcattgc
     1861 cagccccagt ttgggttggt tcatcgccgt gagcagcgcc cagcccagca tgccgaaaaa
     1921 gaccagcatc ccgagacgaa aacgacgccg cgcctgatcc ggtttttgcg tcagcggtga
     1981 ggcggcagaa actttctgca ttttcacggg aatacggaag atgggcagca gggtaaagcg
     2041 cgcgccaaac catgaaccaa tggcagtggc gatggcaaag aaccaggcat gcagcgagaa
     2101 ctgaggaatg ccggtaaaga acgccgccag gttacagccc attgccagac gcgcgccaaa
     2161 accggcgata atgccgccaa taatggcctg cataatgcgg atacggctgc gcggcatgcg
     2221 cagtttgaca ttgttggccc acagcgctgc ggcaaagcag ccgccaaaca tgccgaggat
     2281 catcatcccg tcgatgcggg ttaatggcga tccttccaga tggataattt taaaataacc
     2341 ccactcttca gcatggacgc caaacagctg caggagctga ccgccccaac gggtaaattc
     2401 acccgtgaca gcccaaaagg tgccagtaat gccaaaatag taagtggaga gaatacccgc
     2461 cgcgatgacc gcagggatgg gtgcccagaa tttaatcaac caggcgtgtt tgaattgctg
     2521 ccatgacatg aatgtagcct ctgaactcag aaggtttgaa acaagaatcg cgaatcatac
     2581 atcatgcaaa tttattttcg gggtaataat tcacaaaaat aatagttaga tcaaatttct
     2641 ctctgaacct ggaaaggctg gcgacatcag ggctttaatg ccacaatcct cttttcttct
     2701 catgcaggac gaatgatgaa aaaactcgca attgcaggtg cgctcatgtt gctggcgggc
     2761 tgtgccgaag tcgaaaacta taacaatgtc gtgaaaaccc ccgcgccgga ctggctggcg
     2821 ggctactggc agaccaaagg gccgcaacgt gcgctggtca gtccggaaga gataggcagc
     2881 ctgattgtca ctaaagaggg cgataccctg gattgccgtc agtggcagcg ggtgattgcc
     2941 gtgccgggca agttgacatt gatgtctgat gatctcacta acgttaccgt aaaacgtgag
     3001 ctgtacgagg ttgagcgtga cggtaacacc attgagtacg atggtatgac gatggagcgt
     3061 gtggatcgcc cgacggcaga gtgtgccgcc gcgctggata aagcgccgtt accgacgcca
     3121 ctgccgtaat gaaatctccc cggcttgccg ccggggatga atttaaatca cctcttcgat
     3181 aacccacacg ctgacgctgc cgccgttgca aaagaaggtc gcttcgccgt tttcgtcggt
     3241 cactacgctc tcttgccgat tccccagaaa atcacgccag gttttattgc cgtaattctc
     3301 tcccagatga atggtttttt cgccatcatc cccgttcgac atgaccacca cgcagccggg
     3361 atattcgtca gtgccactgc ggctaaaggc aatgcagttc ggatggtcga aaaataacgt
     3421 ctgtacaccg tgggcgaaac gctgacgggc gagaattaac tcatcaagct gttcgattat
     3481 tggcatatct atcggatagg tttgcccgtc accaccgaca tcttcgtaat gcgcaccgta
     3541 gaggtccgga tagaacaccg aaggaacgcc attttcccgc aacaaaatta gagcatacgc
     3601 cagcggttta aaccacggtt cgaccggcgc ttcgagggct tgcaacggtt gggtgtcgtg
     3661 attggctacg agggttacgg cgtggaaagg atcggcttcc actaatgtac ccgtgaaaat
     3721 ctgcgtcatg tcgtagtcgc gtcccatgcg cgatgcttca tggaatttca tctgcagcgg
     3781 cgcatcgaac agcatggttt tgccttccac ctgatcaata tacgtttgca gtttatcgac
     3841 ttcatgcgac cagtactctg ccacaataaa cagcggcttt ggcgcaactt cctgtacgtg
     3901 ttcgatccac tctttataaa accacgccgg aatatgttta accgcatcaa gacgaaaacc
     3961 gtcgcattgt gtttgttcca tcacccagcg cgcccagtat ttaatctctt ccgtcacggc
     4021 atgattgcgg aaatcgatat tctcacccat cagataatcg aaattaccga gttcatcatc
     4081 aacctgatcg ttccagcctt cgccggtgta gtcgttaaca attttaaata tgccatcttc
     4141 atcaggattt tcgatatggt cgataccgct aaaacatttg aagtcccaga taaactgcga
     4201 gtattgcccg gcacgggcgg ggaaggtgta acgcgtccag ccttcgcact caatgatttc
     4261 ttcatcaatt tgcgtacggt catcagcatt tacacgctgc acgcgaatag cttctttttc
     4321 atccgccccc attttgtggt tgactaccac gtccagtagc accgcaatgt cattacgttt
     4381 cagagcgtca atggcggcca gcagttgtgc tttatcgcca tatttagtag ggatgctgcc
     4441 tttctgatca aactcaccta aatcaaataa atcataggag tcgtagccga ccgaataccc
     4501 gcccgatgcg cctttatagg cgggcggcaa ccagaccata ttgataccaa tatcattaaa
     4561 accgtcggcg cgctcggcca gttcaggcca gagcttaccg ccttccgggt aataccagtg
     4621 aaaacattgt aacagcgtgg gattacgcat tcttcgactc cattcaaggg gaacattaga
     4681 agcgtagccg taatcggatt attcgcgagc catcggctcg ttaagattca aaagaggtta
     4741 tcctgcggag ccagcacaaa gccgccctga tctccatagg cacttaacac tgatttttgc
     4801 accgatgact gaccgaccag tttcgccagt tcatccatac gaatctgtaa taactgcttt
     4861 accttgcttt cgttgtcgag aatcaggcgc agcatcgggc ggagctgctc ctgcatcgtg
     4921 gtggacgggg caacctcttc agtcagatgt gcaatctcct gcaccgcatt cacatacgcc
     4981 atttcgctgg cgatgagttc gtcccattgt tcttccgttg ccaggcgtaa catgagctgg
     5041 cttttatcga cgagttgttg ccaggcgaaa tacaaatgcg gtgcatggtt catcagactg
     5101 ggtcctgaat caaagaaggg gagagtaaag actctttcca ggcatcggca atattgcgca
     5161 ttaatgcttc cacttcttcg actgcggaga catcgttgcg taaattggct tgcagcaagc
     5221 gcctgaccat atagctataa agagcaatca agttttgggt tagttcgtct ttgctctctt
     5281 catcaagact cacccgcagc ccgttctcaa tgatgttgat cgcttttgac aaagagacgc
     5341 ctttgccttg ctgattgttg tcctgcataa acaggcgcgc tctaaccagt gcgctcagca
     5401 ctccatcaaa tagcatggtg accagctgtt gctggctggc gctcattacg gcgctttcga
     5461 cgccaatttg tgcataggct tttgagccgc tcgcggtata catgctgacc tccgtgatca
     5521 ggaattactc attgcagtaa attgttggct gagataggta ctggtgttat tcagtttgct
     5581 catcatggta tctaattgcg taaactgtgc agtgtaacgc gccacggtgt catcaatgct
     5641 ggcactgacg gatagatact gtttggtcag ttttttcagt gtggcgttaa tactgtcctg
     5701 tgcgctatca ataatgccat catcggcaag gtaacctttt acctcggtgg caattttggt
     5761 ggtaattcct gtttctttac catcgcctac cagcaattca cgtacggatg ctgtattttc
     5821 attcaggact tttttgagtt tatcatcatc aattttcagc ttacccgtcg tgccgtcctg
     5881 cttaatcccg atctcgttta atgttttgaa ggcactgtcg ctggcaccgt ttgcaaattg
     5941 tgcgcggatg ccgctttgaa ttgtacgtac aacgctgtct ccgagaagtg caccattatt
     6001 tttatcttgt gcttcagcac ctgggtctac ttctttat
//
LOCUS       DAJTVK010000188         5961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000188 DAJTVK010000000
VERSION     DAJTVK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.190"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            292..368
                     /locus_tag="OO372_003981"
     tRNA            292..368
                     /locus_tag="OO372_003981"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="OO372_003982"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="OO372_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6343336.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="OO372_003983"
     CDS             complement(1357..2247)
                     /locus_tag="OO372_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6343337.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OO372_003984"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="OO372_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCR6343338.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="OO372_003985"
                     /pseudo
     CDS             complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="OO372_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1354 a         1564 c         1556 g         1487 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga t
//
LOCUS       DAJTVK010000189         5837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000189 DAJTVK010000000
VERSION     DAJTVK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.191"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO372_003986"
     CDS             complement(<1..242)
                     /locus_tag="OO372_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6343339.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(604..3387)
                     /locus_tag="OO372_003987"
     CDS             complement(604..3387)
                     /locus_tag="OO372_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6343340.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3444..5816)
                     /locus_tag="OO372_003988"
     CDS             complement(3444..5816)
                     /locus_tag="OO372_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6343341.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1487 a         1321 c         1312 g         1717 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatagc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaaataa caaaatccta atgttattta tcgtgagata
      361 ttgcgagaat aatatttttt cattgaaaaa caatataata tgaaattctt gggtggtggt
      421 aaggtgtttt atgctgttat ttttatgcgc attctgtgcc tcctgaattt tcacgtaaaa
      481 atcagacctt aaaatatcac tattagtacc tggttattat tttgaacgca tttataaaat
      541 tattacataa aaatggcgaa tattgctaaa atccccgcca acgatgtgtt gacggggctg
      601 ttattatttt ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt
      661 aacggcagtg ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca
      721 tgctgcagga tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac
      781 gctacgctgt tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg
      841 ctcactgatc cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc
      901 atcatgtcgt tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg
      961 atcaaccgag aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac
     1021 gcgtagatct tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt
     1081 aacaggtgtc cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg
     1141 ttcttttaca gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa
     1201 tggcgaatca gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc
     1261 ttctgagaca ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg
     1321 atcggcagcc agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt
     1381 cgtgcggtca tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg
     1441 ctggtcgagc gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc
     1501 gttaatcgtg ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt
     1561 agtccgcgcg ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac
     1621 cgaattttcc gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc
     1681 gcttactgct ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc
     1741 gcctttatta gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag
     1801 aataaccctg gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt
     1861 gctgctaatt tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa
     1921 gatgtaagcc gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag
     1981 agacaatgct tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg
     2041 gttctttctt aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag
     2101 acgtttcgaa agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct
     2161 ggatgccagg cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt
     2221 tttcagccag gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg
     2281 ctgatcaatg gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc
     2341 tcgtgcattt acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc
     2401 agtgccgcca gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt
     2461 gaacagctga actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac
     2521 ttgtaccatt ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt
     2581 aatgatatta aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc
     2641 tttgttagcc ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc
     2701 gatatcgccg accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg
     2761 gcgcaattgt gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc
     2821 taaattacgg gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc
     2881 atcctgatgg gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat
     2941 ttcaaaggtt gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg
     3001 tttctgcgta gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt
     3061 aacatcgcga ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg
     3121 ccatgttttt gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt
     3181 gtcttcttcc tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg
     3241 ttgagcatgc ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt
     3301 ttcatcctgc ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa
     3361 cgtcagtaag aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac
     3421 ccggttaagt aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg
     3481 ccactggcgt aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt
     3541 acattgagaa tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag
     3601 gacaagcggg tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa
     3661 tctgaatatt cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc
     3721 gcttcttgcc aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc
     3781 atgtttaagg agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag
     3841 accagattac cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg
     3901 tttaacgaca agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct
     3961 acttggcgga tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg
     4021 gttttgccat cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg
     4081 cttttgctga tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc
     4141 ttaccgattt tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc
     4201 tgataacgcg tcagagtttt attacctgat accgattccg tccagctatt gcggatatca
     4261 cgtaatccca tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt
     4321 gaggtttgat cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt
     4381 gacagatagt tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta
     4441 atgtgctccg ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg
     4501 taaatggtat ggttagtttt ctttccggca gcattaatca cataggattc agactggtta
     4561 tggcgttcag tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa
     4621 acatcaccca cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca
     4681 gcctgggaaa tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta
     4741 catgaaagtt cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc
     4801 caaccaacgg tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga
     4861 ccataggatt tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca
     4921 cggctggagc cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag
     4981 gtaaatttgc tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg
     5041 ccgtcattcg aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg
     5101 gtaacgccga agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta
     5161 aaatctgggg agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc
     5221 tcatcgatat gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg
     5281 ctatcatcag cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca
     5341 aattcaaccg gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga
     5401 taatacccct gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct
     5461 gggttcaggt tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac
     5521 gcaccgtgaa tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc
     5581 gttgaatcca tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg
     5641 gtcggtaaac gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg
     5701 acaggagcat agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg
     5761 gcctgcgcca catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat
     5821 ttatatctcc gtacagc
//
LOCUS       DAJTVK010000190         5640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000190 DAJTVK010000000
VERSION     DAJTVK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.192"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO372_003989"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO372_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6343342.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO372_003990"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO372_003990"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6343343.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO372_003991"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO372_003991"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6343344.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO372_003992"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO372_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6343345.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAJTVK010000191         5617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000191 DAJTVK010000000
VERSION     DAJTVK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.193"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            13..429
                     /gene="soxR"
                     /locus_tag="OO372_003993"
     CDS             13..429
                     /gene="soxR"
                     /locus_tag="OO372_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6343346.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            975..2324
                     /gene="ghxP"
                     /locus_tag="OO372_003994"
                     /gene_synonym="yjcD"
     CDS             975..2324
                     /gene="ghxP"
                     /locus_tag="OO372_003994"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6343347.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2476..4125
                     /locus_tag="OO372_003995"
     CDS             2476..4125
                     /locus_tag="OO372_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6343348.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4279..5570)
                     /locus_tag="OO372_003996"
                     /pseudo
     CDS             complement(4279..5570)
                     /locus_tag="OO372_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1258 a         1270 c         1456 g         1633 t
ORIGIN      
        1 acccccggcg aagtggcgaa acgcagcggt gtggcggtat cggcgctgca tttctatgaa
       61 agtaaagggt tgattaccag tatccgtaac agcggcaatc agcggcgata taaacgtgat
      121 gtgttgcgat atgttgcaat tatcaaaatt gctcagcgta ttggcattcc gctggcgacc
      181 attggtgaag cgtttggcgt gttgcccgaa gggcatacgt taagtgcgaa agagtggaaa
      241 cagctttcgt cccaatggcg agaagagttg gatcggcgca ttcatacctt agtggcgctg
      301 cgtgacgaac tggacggatg tattggttgt ggctgccttt cgcgcagtga ttgcccgttg
      361 cgtaacccgg gcgaccgctt aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa
      421 caaaactaaa gcgccacaag ggcgctttag tttgttttcc ggtctttgtc tttctctcta
      481 tcccgctggt acacaggagg gtttcccccg acgtcaacac accccattcg agcacgtggt
      541 ggaggttccg gttggtgttg atgctttaat tgtatgtcac cggcatttct tcgccagtgt
      601 aaaagtatac tttttatccg caatattttt gtcatctcag acgatttttt atcgcaatcc
      661 tgaacggtat acggctcgat aacgctgcaa tcttgcgcac cgacgataac gtttgcgcat
      721 caattgcctg gtttttcatc gtcaagacaa taaaagagaa aaaagcagca aacttcggtt
      781 gaaaaagccg ctatgatcgc cggataatcg tttgcttttt ttaccacccg ttttgtatgc
      841 gcggagctaa acgtttgctt ttttgcgacg cagtaaattg tcgcaaacct ggagcaggaa
      901 gataacgttt ctctggcagg ggattgtccg ccacgcatct tgacgaaaat taaactctca
      961 ggggatgttt tcttatgtct acgccatcag cgcgtaccgg cggttcactc gacgcctggt
     1021 ttaaaatttc acaacgtgga agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt
     1081 ttctggcgat ggtctactcg gtcatcgtcg ttccaggtat gttgggtaaa gcaggcttcc
     1141 cgcctgcggc agttttcgtt gcaacctgtc tggttgccgg actcggttct atcgtgatgg
     1201 gtctgtgggc taatctgccg ttggcgattg gttgcgccat ctccctgacg gcgtttaccg
     1261 cattcagcct ggtgctgggg caacatatta gcgtacctgt cgcgctgggt gccgtgttcc
     1321 tgatgggtgt gctgtttacg gtaatttctg ccacgggtat ccgtagctgg attttgcgca
     1381 acttgccgca cggtgtggcg cacggcacgg ggatcggtat cggtctgttc ctgctgctca
     1441 ttgccgctaa tggtgtcggt ctggtgatta aaaacccgct tgatggtctg cccgttgcgc
     1501 tgggtgattt cgcgaccttc ccggtgatta tgtcactggt aggtctggcg gtgatcatcg
     1561 gcctggaaaa actgaaagtc cctggtggca ttctgctgac cattatcggt atctcaattg
     1621 tcggtttgat cttcgatcct aacgtccatt tctccggcgt tttcgccatg ccttcattga
     1681 gcgatgaaaa cggcaattca ctgattggca gcctggacat tatgggcgcg ctgaatcctg
     1741 tagtcctgcc aagcgttctg gcgctggtga tgacggcagt atttgatgcc accggaacta
     1801 tccgtgccgt cgccggccag gcgaacctgc tggataaaga tgggcagatc atcgacggtg
     1861 ggaaagcact gaccactgac tccatgagca gcgttttctc tggcctggtg ggtgctgctc
     1921 cggcagcggt atacatcgag tctgcggcgg gtacggcggc gggcggtaaa accggtttga
     1981 cggctatcac cgttggcgtg ctgttcctgc tgattctgtt cctctctccg ctctcttacc
     2041 tcgttccggg gtatgcaacg gctccggcgc tgatgtacgt tggcctgctg atgctgagca
     2101 acgtggcgaa aatcgacttt gctgattttg ttgatgcgat ggcgggtctg gttacggcgg
     2161 tattcatcgt gctgacctgt aacatcgtaa caggcatcat gatcggcttc gcgactctgg
     2221 tgattggtcg tctggtttcc ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg
     2281 ccgtggcgct ggtgaccttc tatgcgggtg gctgggctat ctaatctttc atcggattct
     2341 gaaaacgggt ggcaatggct gcccgttttt attttctccg cacattgtgt gagcttttgc
     2401 gatattctga aaaaatgaga attcaggcat aacgcctttc tcagatcata aaaaaacatc
     2461 gcaaacaggg aacgcatgga aatcttcttc accatactga taatgaccct cgtggtctca
     2521 ctgtccgggg tggtcactcg tgtcatgccc tttcagatcc cgcttccgct tatgcaaatc
     2581 gccatcggtg cgctactggc gtggccgacg tttggtttgc atgtggagtt tgatcctgaa
     2641 ctctttttag tcttgtttat cccgccgttg ctgttcgctg atggctggaa aacgccgacc
     2701 cgtgaatttc tcgaacatgg tagagagatt ttcggcctcg cgctggcgct ggtggtggtc
     2761 accgtggtcg gcattggctt ccttatttac tgggtggtgc cgggcattcc gctgatcccc
     2821 gcctttgcgc tggcggcggt actttctccg accgatgctg tggcgctctc cgggattgtt
     2881 ggcgaagggc gcatcccgaa aaaaatcatg ggcattttgc agggcgaagc gttgatgaat
     2941 gacgcctccg gcctggtgtc gttgaagttt gccgtggcag tggcgatggg gacgatgatc
     3001 ttcaccgtcg gcggtgcgac ggtcgaattt atgaaagtag ccattggtgg tattcttgcc
     3061 ggttttgtgg tgagctggct gtatggtcgc tcgctgcgat tcctcagccg ctggggcggt
     3121 gatgaacccg cgacgcagat cgttctgctg ttcttgctgc cattcgcttc ttatctgatt
     3181 gccgaacata ttggcgtttc cggcatcctc gctgcggttg ccgccgggat gaccatcacc
     3241 cgctccggtg tgatgcgccg tgcgccgctg gcaatgcgcc tgcgcgcaaa cagcacctgg
     3301 gcgatgctgg aatttgtctt taacggcatg gtgttcctgc tgttaggtct gcagctgccg
     3361 ggtattctgg agacgtcgct gatggcggca gaaatcgacc ctaacgtcga aatctggatg
     3421 ctgtttaccg atattattct gatatatgcg gcgctgatgc tggtccgttt cggctggctg
     3481 tggacgatga aaaagttcag caaccgcttc ctgaagaaga agccgatgga gtttggttcg
     3541 tggaccacac gagaaatcct gatcgcgtct ttcgccgggg tgcgtggggc gatcactctg
     3601 gccggtgtgc tctctatccc gctgctcttg ccggatggta acgtcttccc ggcgcgctat
     3661 gagctggtgt tcctggcggc tggcgtcatt ctcttctcgc tgtttgtcgg cgtggtgatg
     3721 ttgcctattc tgctacaaca cattgaagtc gcggaccatt cgcaacaatt gaaagaagaa
     3781 cgtattgcgc gagcggcaac ggcagaagtg gcgattgtgg cgatccagaa aatggaggag
     3841 cgtctggcgg cggataccga agagaatatc gataaccagc tgcttacgga ggtcagttct
     3901 cgcgtcattg gtaacctgcg tcgtcgcgcc gatggacgta acgacgttga aagttccgtg
     3961 caggaagaga accttgagcg tcgcttccgt ctggcggcat tgcgttctga acgtgctgaa
     4021 ctgtaccacc tgcgcgccac gcgggagatc agcaacgaaa cgctgcaaaa attactgcac
     4081 gatctcgatt tgcttgaagc gttgctaatt gaggaaaatc agtaagccga aagcgacgta
     4141 accaggcccg aggtaatctg aaaaaggtgc aatcttttgc acctttaaca ggttagctcg
     4201 ggctaacatt catgattcta aaacaaataa gttatctctc ttgtaaagtc aattcattgg
     4261 ctgttcttcg acttaacctc aatctgcgtg ttgcataata tctgcagtaa tcttcatagc
     4321 gactgcatta atattatcct cagtttgtga catgccagta tgagaggtac aatcttcttc
     4381 aaagaatttc tgtagtgtta ccagggtatc actatcaatg gggatatttt cctccccacg
     4441 gagtatggaa atgcccattc cctggacgct agcctcatca aaattaaccc gatagagacg
     4501 ggtattaaac atacaggctt ttgttaaaat agaacagttg aaatggcagt tgcttaaatt
     4561 ggcattatta aaattaacat tagccatatt agagtcggta catataacca tttcgaggaa
     4621 tgtatttgtt agccacgtac aaaagagttt gctatcaatt aagtttatat tctggaggat
     4681 tgctttgttc agattgacgc gagacagatc agcatgttca aagttaatta aatcgagttt
     4741 agagaaacta aggtcggctt taaataaatt aacatatggc gctattactt tataaagatt
     4801 tgcctcagcc atgtacgcca tataaaaatt tgaatagtca agaaaggtat ttttaatatt
     4861 ggctctattc attgctgcat tgtagaattg aacatgcata acggagcatg catttagagt
     4921 tccgtcggcc atttttatct gagcgaaatt tgagtcatcg agaatacaat tatcaaaaag
     4981 tatttttcca ttatggcgta ataaaaattc gttttataca tattacagtc ctggaaaata
     5041 gcgttttgta gtctgcaacc tgcaaaattc gaattggata aatcttcatc tttaatcacg
     5101 atacctttca ggctaaggtg tgaaaaatcg taaccaccat ttactgtctg tggaatttcg
     5161 atagtattat tatggacggc gacattattt ataaaatata ccaccttaat ggatacttcc
     5221 ttttctttac taaggtaaac tcttctcgta attctatcgt cacatttatt atttgcatat
     5281 aaagctatat ctataaaatg tttatgtatt ttcctgtccg tttccttcgt gatttttcct
     5341 gatggtaaac tatcataaat tcttttcatt ttctgaatca aacctatcac tttttcatga
     5401 tcatccatct tgaaaagaga gagggcttta ttgtcaatta acgtgttttt atgatgtttg
     5461 ctgtaattat atgattttgt tttctttgta tgtgatgaac ttgtgaaaga gtggttatcg
     5521 ttaatctggc aaagagagaa aaacatatta tttaaaccat tatagcgcat aaaatatcct
     5581 tataattaac aagaaagaaa aggcatattc tctgcat
//
LOCUS       DAJTVK010000192         5615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000192 DAJTVK010000000
VERSION     DAJTVK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.194"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..513)
                     /locus_tag="OO372_003997"
     CDS             complement(<1..513)
                     /locus_tag="OO372_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6343349.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="OO372_003998"
     CDS             complement(759..1211)
                     /locus_tag="OO372_003998"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6343350.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO372_003999"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="OO372_003999"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6343351.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO372_004000"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="OO372_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6343352.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="OO372_004001"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="OO372_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OO372_004002"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="OO372_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6343353.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5615)
                     /locus_tag="OO372_004003"
     CDS             complement(5494..>5615)
                     /locus_tag="OO372_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6343354.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1467 c         1458 g         1351 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcga
//
LOCUS       DAJTVK010000193         5479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000193 DAJTVK010000000
VERSION     DAJTVK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.196"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..650)
                     /locus_tag="OO372_004004"
     CDS             complement(279..650)
                     /locus_tag="OO372_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343355.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(711..1208)
                     /locus_tag="OO372_004005"
     CDS             complement(711..1208)
                     /locus_tag="OO372_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343356.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(1284..2072)
                     /gene="aadA1"
                     /locus_tag="OO372_004006"
     CDS             complement(1284..2072)
                     /gene="aadA1"
                     /locus_tag="OO372_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6343357.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(2130..2654)
                     /gene="sat2"
                     /locus_tag="OO372_004007"
     CDS             complement(2130..2654)
                     /gene="sat2"
                     /locus_tag="OO372_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6343358.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(2749..3222)
                     /locus_tag="OO372_004008"
     CDS             complement(2749..3222)
                     /locus_tag="OO372_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6343359.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3554..4090
                     /locus_tag="OO372_004009"
     CDS             3554..4090
                     /locus_tag="OO372_004009"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6343360.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            4205..4531
                     /locus_tag="OO372_004010"
     CDS             4205..4531
                     /locus_tag="OO372_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6343361.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(4719..4958)
                     /locus_tag="OO372_004011"
     CDS             complement(4719..4958)
                     /locus_tag="OO372_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6343362.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         1426 a         1307 c         1219 g         1527 t
ORIGIN      
        1 tctctctaga tagcagggat ttcttggcct tgcctcatgc tgatagcttg taaatatcac
       61 acgatcctta gccccacaaa gtgagcatac tccgtagctt tcatttatag aaatcgtcgt
      121 tagattgtcg cagcatgtgc acgttccgac tatttgctca ggaagcatcg ccataccttg
      181 gcctaggtaa agaagatcag actcgtagcc acatcggtgg caactgagct tgtacgcaat
      241 gcccatctgg ttgtctcacc tgaatcgtaa cgtcagagct attcatccat ccattcggca
      301 tcaatgtatg ccactaagcc gaccagctga ccgtttggac tgaagtatgt tgcctgcacc
      361 ccacttgaca tctcatcaat accaccgcac tgcgctaggc gccggtccca cttgcccaca
      421 gaagcgtcag gatcggctga gttccccaag acccttgtga gccatgcctc ctttaactca
      481 acctctcgaa tgattgcgtc ggccagctcc tccgcgttat ctagttcgac atagtctgga
      541 attcggacgt acgttctgaa cacagctgga tacttacttg ggcgattgtc atacatgaca
      601 tcaacaatgt acccgtttgt gtaaccgtct cttggaggtt cgtatgacac tagtggttcc
      661 cctcagcttg cgactagatg ttgaggccta acattttatt agagagcagg ctagttgctt
      721 agatacatga tcttcaggcc gttatctgtc agggcaagcg aaaattggcc atttatgacg
      781 accaatgccc cgcagaagct cccatctttg ccgccataga cgccgcgccc cccttttggg
      841 gtgtagaaca tccttttgcc agatgtggaa aagaagttcg ttgtcccatt gttggcaatg
      901 acgtagtagc cggcgaaagt gcgagaccca tttgcgctat atataagcct acgatttccg
      961 ttgcgactat tgtcgtaatt ggatgaacta ttatcgtagt tgctctcaga gttgtcgtaa
     1021 tttgatggac tattgtcgta attgcttatg gagttgtcgt agttgcttgg agaaatgtcg
     1081 tagttggatg gggagtagtc atagggaaga cgagcttcat ccactaaaac aattggcagg
     1141 tcagcaagtg cctgccccga tgccatcgca agtacgaggc ttagaaccac cttcaacaga
     1201 tcgcgcatag tcttccccag ctctctaacg cttgagttaa gccgcgccgc gaagcggcgt
     1261 cggcttgaac gaattgttag acattatttg ccgactacct tggtgatctc gcctttcacg
     1321 tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttgtccaaga
     1381 taagcctgcc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
     1441 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
     1501 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
     1561 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
     1621 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
     1681 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
     1741 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
     1801 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
     1861 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
     1921 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
     1981 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
     2041 gtcgatactt cggcgatcac cgcttccctc atgatgttta actcctgaat taagccgcgc
     2101 cgcgaagcgg tgtcggcttg aatgaattgt taggcgtcat cctgtgctcc cgagaaccag
     2161 taccagtaca tcgctgtttc gttcgagact tgaggtctag ttttatacgt gaacaggtca
     2221 atgccgccga gagtaaagcc acattttgcg tacaaattgc aggcaggtac attgttcgtt
     2281 tgtgtctcta atcgtatgcc aaggagctgt ctgcttagtg cccacttttt cgcaaattcg
     2341 atgagactgt gcgcgactcc tttgcctcgg tgcgtgtgcg acacaacaat gtgttcgata
     2401 gaggctagat cgttccatgt tgagttgagt tcaatcttcc cgacaagctc ttggtcgatg
     2461 aatgcgccat agcaagcaga gtcttcatca gagtcatcat ccgagatgta atccttccgg
     2521 taggggctca cacttctggt agatagttca aagccttggt cggataggtg cacatcgaac
     2581 acttcacgaa caatgaaatg gttctcagca tccaatgttt ccgccacctg ctcagggatc
     2641 accgaaatct tcatatgacg cctaacgcct ggcacagcgg atcgcaaacc tggcgcggct
     2701 tttggtacaa aaggcgtgac aggtttgcga atccgttgct gccacttgtt aacccttttg
     2761 ccagatttgg taactataat ttatgttaga ggcgaagtct tgggtaaaaa ctggcctaaa
     2821 attgctgggg atttcaggaa agtaaacatc accttccggc tcgatgtcta ttgtagatat
     2881 atgtagtgta tctacttgat cgatcaggct tttgtatatc tccccaccac ctgaaacaat
     2941 gacatgatcc gttattttct ttaggttggt taaagcatct ttaattgatg gaaagatcaa
     3001 tacgttctca ttgtcagatg taaaacttga acgtgttacg accgcatact ttcggttggg
     3061 taatgctccc attgattcaa aagtcttgcg tccaaccaac agccattggt tataggtaat
     3121 agctttaaac aggagctgtt cacctttggc actccatgga atatcagggc cattcccgat
     3181 aactccattc ttcgatatag ctaccattag tgatagtttc acaattcttc ctcagaggtt
     3241 aacattttat tagtaagcat gctcgttttg tcacccgctg atgcttaccg ttaattaata
     3301 taatcacttg tattaaatag actttcttta aaaatacaag acactctgtt attacaaatc
     3361 gtgcatgccg tctatcctgc ttgcacgatg cacattatct cacccaaaga actcataaca
     3421 ttttaattta tttagcatta aattaaaact ctaccggtaa acaagcatct ctaggcgtaa
     3481 aaaacaggca cttttttaaa tcccagctag aataggctgt ataggcagac agttgcaaga
     3541 caaggagcgt tttatgtcta acagtccatt tttaaattct atacgcacgg atatgcgaca
     3601 aaaaggttat gcgctgaaaa ctgaaaaaac ttacctgcac tggattaagc gttttattct
     3661 gtttcacaaa aaacgtcatc ctcagaccat gggcagtgaa gaggtcaggc tgtttttatc
     3721 cagcttagca aacagcagac atgtagccat aaacacgcag aaaatcgctt taaatgccct
     3781 agcttttttg tacaacaggt ttttacaaca gccgttgggc gatattgatt atatccctgc
     3841 aagcaagcct agacggctac cctctgttat ctctgcaaat gaagtgcaac gcattttgca
     3901 ggttatggat actcgcaacc aagttatttt tacgctgctg tatggtgcag gtttgcgcat
     3961 taatgaatgc ttgcgtttgc gggttaaaga ttttgatttt gataatggct gcatcactgt
     4021 gcatgacggt aagggtggga aaagcagaaa cagcctactg cccacgcgcc taatcccagc
     4081 aataaaataa ctcattgagc aagcgcggct tattcagcaa gacgacaact tacaaggcgt
     4141 agggccatcg ctgccttttg ctttagatca caaataccct tctgcttatc gacaagcggc
     4201 gtggatgttt gtctttccct ccagcacgct ctgcaaccac ccgtataacg gcaaattatg
     4261 ccgccatcat ctgcatgact ccgttgcgcg aaaggcattg aaggcagccg tacaaaaagc
     4321 aggcatcgtt agcaagcgtg tcacttgtca tacatttcgt cactcgtttg ctacgcatct
     4381 attacaagcg gggcgtgata ttcgcactgt gcaagaactc ttagggcata acgatgttaa
     4441 gaccacgcaa atctatacgc atgtgttggg tcagcatttt gccggcacca ccagtcctgc
     4501 ggatggactg atgctactta tcaatcagta aaagtagata aatatcttta tgtagccctg
     4561 agtagcccct gatacttttt atggtgaaaa agataaacaa tcatagaggg gataaatatg
     4621 agccagttct accattgttc agataagttt aagcaagata ctgtcaatca cagtgaccat
     4681 tcatggtcat tacacttcct ccagttgcca gacaatgcct aacataaaaa cgcaaaagcc
     4741 caaaagtcag agcttaggtg ttctaggcgt tttctaagcg ctgaagaaat acgtcttacc
     4801 gtagattgcg tgcgtttctg aagcacattt tccttataca tggctttatc ccaggcagct
     4861 tcatcaagct cttggagtaa caagccagta ataatgcgac actctcgggt ttgtagcgag
     4921 ccgctggtga gatcggagtt atagcggaaa ggggtcattt gtaattattt gtgggtggag
     4981 gatagttaac ttattattgt agttggaaga ggctgctttc caaacattag cacctcgtaa
     5041 tgcttgttac tgtatattaa tttatgctct tagcgcatgt taatgggcta ttacaatgca
     5101 taatgtccac atcaagataa ctgtggctta acacctgtac aagcgaaaaa aatcaatcac
     5161 ctgtccggaa atacgggatc actgtaaagt gttggtgagg cagtctagga gggggattta
     5221 atttgggata ctgatgaaag gattatgatc agttggatat aggatcgtac taagctgagc
     5281 ggcaggttag ctagattagt taaccagata agtgaaatct agttccaaac tattttgtca
     5341 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     5401 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     5461 gcccacaagg gctgccgac
//
LOCUS       DAJTVK010000194         5339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000194 DAJTVK010000000
VERSION     DAJTVK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.197"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..405)
                     /gene="rrrD"
                     /locus_tag="OO372_004012"
     CDS             complement(<1..405)
                     /gene="rrrD"
                     /locus_tag="OO372_004012"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCR6343363.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQL"
     gene            complement(405..620)
                     /locus_tag="OO372_004013"
     CDS             complement(405..620)
                     /locus_tag="OO372_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6343364.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1301..1375)
                     /locus_tag="OO372_004014"
     tRNA            complement(1301..1375)
                     /locus_tag="OO372_004014"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1340..1342),aa:Gly,seq:tcc)
     gene            complement(1379..1454)
                     /locus_tag="OO372_004015"
     tRNA            complement(1379..1454)
                     /locus_tag="OO372_004015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:tgt)
     gene            complement(1457..1533)
                     /locus_tag="OO372_004016"
     tRNA            complement(1457..1533)
                     /locus_tag="OO372_004016"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1497..1499),aa:Arg,seq:tct)
     gene            complement(1535..1611)
                     /locus_tag="OO372_004017"
     tRNA            complement(1535..1611)
                     /locus_tag="OO372_004017"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1575..1577),aa:Ile,seq:cat)
     gene            complement(1801..2364)
                     /locus_tag="OO372_004018"
     CDS             complement(1801..2364)
                     /locus_tag="OO372_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6343365.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(2468..2686)
                     /locus_tag="OO372_004019"
     CDS             complement(2468..2686)
                     /locus_tag="OO372_004019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343366.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(2845..3231)
                     /locus_tag="OO372_004020"
     CDS             complement(2845..3231)
                     /locus_tag="OO372_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6343367.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(3221..3592)
                     /locus_tag="OO372_004021"
     CDS             complement(3221..3592)
                     /locus_tag="OO372_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6343368.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(3592..3849)
                     /locus_tag="OO372_004022"
     CDS             complement(3592..3849)
                     /locus_tag="OO372_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343369.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(3846..5237)
                     /locus_tag="OO372_004023"
     CDS             complement(3846..5237)
                     /locus_tag="OO372_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6343370.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(5234..>5339)
                     /locus_tag="OO372_004024"
     CDS             complement(5234..>5339)
                     /locus_tag="OO372_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6343371.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT         1311 a         1453 c         1193 g         1382 t
ORIGIN      
        1 tagctggtca catgcgcctt tgatatcgcc ctggtttatt ttgcgaagaa gcgtcgatgt
       61 tctgaaattg ccagcgccca cgttgtaaac gaacgagtaa agagcgccgc gcgttgtttc
      121 cggtatatcg actttgatgt acgggttaat ttgtctggcg acagtggcaa ggtctttatt
      181 caggagggct ttgcattctg cttcggtata cgttttaccg agcatgatgt cttttccggt
      241 gtgtccgtga catacagtcc atacaccaat gatatctttg tatggtatgt agctgacacc
      301 ttccagacca tcgttaccac ctgggccagt gattaacaca gatgctatag caacagcccc
      361 accaccaata gcagctgcaa cagccttgcg taatgatggc gacattattc acctctcgca
      421 gccttacgct tgtcttctct tattttgaaa tacagatttg tcagataagt cagaagcccc
      481 agaagcagac ttcccagcac accaatcgca gcccactgtg atggactgac ctgatccaac
      541 cactgcaaaa accagtagcc ggcactgccg gcggaggtgc cgtaggcaat gcctgttgat
      601 attttttcca tctgatacat atcccgcccc ccgacagacc tgtgctatcg gaaagaaaaa
      661 aggccatcag caaaactctg atggcctgaa tcacctttac caatattgta tgaaaaaaca
      721 cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta gcattcttaa
      781 ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat ccccaatgtt
      841 tttgctggcg ggtccttttt tctacggtcc tcctccccgg aggggggcat ttcattattt
      901 ttctaatcgt tactggtgag aaggcggaag aaaaagccag ttcttcgccc ctaaataaca
      961 gcatgattcc agtccatgca aaatctgatt tccagctcac acataccgag cgctgtacaa
     1021 aaaaccgcca gccaaggcag ttaagagtgt gttcccgggg tttgcttaga atattttgta
     1081 agttgtccga agagatattt acaacaccag aatgatgaat catcagtccc ctgccagagg
     1141 ttcactacgt aacctgaccg acaatgcacc attctggtgc tgaaaaaaga gcactggaac
     1201 tgcaacaaaa caacatcaca gaacaggaga tggtaaggag tcagacattc ccacacaata
     1261 ttgtgtcagt gcaaataaca accttcgtct caaatctcgc tggagcgggc agcgggaatc
     1321 gaacccgcat catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatatg
     1381 gtgccgacta ccggaatcga actggtgacc tactgattac aagtcagttg ctctgcctgc
     1441 tgagctaagt cggcgctggc ccaccaccga ggactcgaac ctcgcaccgt caacttagaa
     1501 ggttgatgct ctatccggat gagctagtgg tggttggtgg cccttgctgg acttgaacca
     1561 gcgacctggc gattatgagt cgctcgctct gaccaactga gctaaagggc cggaggcaga
     1621 ataataacca tatgtcatca catctgcaaa ctcatctgac caccagcacg tttaacgtcc
     1681 tgtgccgttt ttcaggtata aaaaaacccg ctcatcggcg ggtttaagct gtgtggcgta
     1741 gtaaccactc ttaacaggat attcaacttt ttacgatcgt aaagttttcg gaaaaaattt
     1801 ttaaaaccgc atcaaccgct ccaccagttg ctctttacgg gcaacgatcc acccgtgttg
     1861 ctccagataa aatttaaacc gttccagagt acataccatc gcatcggcgg gtaccttttc
     1921 cgtgaactca acctgaccgt gtttatcgaa gtggctcagt aatgcgcatc catcattttc
     1981 gatagatgtg ttttgtgccg ctggtggctg tttctggttg aaataacagt cttccagttt
     2041 ttcgaacact tcccacgcct gatcggtttc gagcattttg gcgtgacggg ctgcgccacg
     2101 ttctgtccag aggatgaggg agcgaacgtt acgggcaatt ttcacagaaa ctcttaaaga
     2161 gttttcgttc ttcaactctc gtaacgcatc accttctatc aggaaaaaat gtttaccttc
     2221 tataaaccgt tctttgtttc gattgaaatt gtttgtcaaa cgctggcgct ctgttccgta
     2281 caaatcagcc agcagctctg ttgtcatgac aggaagatgg ttatgcgtaa gtgacgggag
     2341 tttttcaata gacgtgcgat tcatagacat gtcctttcga atattttgat ttaccccttt
     2401 tgagagggtg accgggcgct caaaaccgtc gaaagtcggc gggcgtattt ccattgctgg
     2461 tattgtatta gccgcacacc cggtcataaa ccaagaattc tggacacaaa aaaaccacat
     2521 ggctatcggg tgtggatacc gctttcgagg tgttttgagc accgcgggga aatgtaaact
     2581 gcaaacctga aactgtcaac accgtcaggt tccaatcatc gggtggtgag acgcacaggg
     2641 ttggaactac cgggaaaccg accggcgagc ttttcagctc ccccatgcgc cccaccataa
     2701 ttcagatgtg cgcgtgcata cgacaataaa aaacacgctc gcggcgtgtg tctgtcgcgg
     2761 tctctatccg gggttccaat cccgacgcca gattttgctg gcgcgtgagg aatatagccc
     2821 cggacaatgt gtctggtcaa gcacctacat aattcgttct acgtatctgt ccatctccag
     2881 ccggatatca agcatcatca acatgccatc aataacccct tccgctttct gcaggcgctt
     2941 gccaatacag gtatccgaac acccatgttt ccgtgccaga ctcataaaag tcattccacc
     3001 tacgtaataa tccaccagca aatcgtgcaa atcctgattt ttcttgttca accgggccat
     3061 acagccacaa attatcattg catcatcatc agaacactga gggcgtgatt tcactttcga
     3121 cggaattaac cctttaaaac cagcagcgat cgacgcccag gatacatctt catgagtgtt
     3181 tgcagcccaa gccccccacc gttccataac ctgctgaata tcacgcgcca tcgttatcac
     3241 ctgtgatttc ataaatcttc acgcccagtc gcccaccagg aacgagctga ccgcgcacaa
     3301 tattgatttc atcaaactgc tcgtcgtcta tgagtagtcc ggcatgcgtc agcgcatcca
     3361 gtggtgcttt caggatattg tccaggtcac gacggcgctt atccggtggc tctgcaataa
     3421 tctttatcgc cagccttccg gacaggttta atttcagccg ctgctggcga acaattagcg
     3481 tcacatcacg gcgataacgc tcaccaactt ttgatacaaa atatgtgctg ccacgacgtc
     3541 gccagtaagt attcaccgtc ggcggataag gtaaaataaa ctcatggcgc atcagcgcag
     3601 cacctcctgc accatttttt caaactttcc aactttggtt tccagctctg ccacacaatc
     3661 aacgagctca tccaccgctt tttgtgcgcg atgtttcgcc tgcatcagtt ccctgagcgc
     3721 tggcaccata tcccgacgaa tggcatcttt tgttacacct gttttttcca gttgttccgc
     3781 ctgtcgcaac atttcctgcg cgtgtttacg caattgttca gaggtaaaag tcattgtctg
     3841 gttgttcaaa agaaacgctc catcttactg ctgtcggtac gtgtattgct gtatctgcgc
     3901 ggctggggct gccgcattgg ggtggaaaga acctgtgcgc tttcctggtc cacaggcaga
     3961 aaatgtccgt tataaaaacg ccggtaaatc gtccccagag aaccgttacg ttgtttcgtg
     4021 atattaattt ctgctatgcc tgtagcctgt gtatccgggt tgtacacttc atccctgtaa
     4081 agcatcagaa tgatgtctgc atccgcctct atttctccgg aatttttcag gtctgagttc
     4141 atgggacgtt tattgggtct ggactccaca ccgcgggaga gctggctcag cgcaatcaac
     4201 ggaaaaccac cggattttgc caggccttta agcccctttg agatttcacc cacggcaagg
     4261 tcatgacgcc ccgtggttcg ggtttttatc agcccgagat aatcaaccac caccagcgcc
     4321 gtttccggat gtttaatcag atggtgtttc gttgttgcgc atatctcatc aatggtcagg
     4381 ttcgcctggt ccaccatcca gatattgcgc ccggtcatcc gccccacccc ttgtgagaaa
     4441 cgcgcccagt cttcatcttc aaagtgagcc acagatttca ggcgtgatac tggcatccct
     4501 ccagccgcag acaccatacg ttcaccaatc tggatgttcg ccatctccat ggtgaacaga
     4561 agcacaccat gcccctgctc agtcaccttg tcgatgatgt ccagcgcaag ttcggttttc
     4621 cccatcgaag gacgggcggc aatgaatacc aggtctccgg gctccatacc gcctgttttt
     4681 gcgtccagtt catcaatacc ggtcatcaac gtcctggatt tctccagccc ctgattccgg
     4741 cattcaacac gctcaaccac ttccggaagc acatcatcaa tatgtaccgg ctgaatgacg
     4801 ccctttcctg tcgacagtgt gaccatcatg ttctgcgcat ccttcagggc atcttcagct
     4861 gcttcacagg tatgcgcatc acgtaatttc tgcagcgcct cattcagtgt tttttctgca
     4921 tcgcgcagtg cggcattgcg ccgcaacgct gcaacatagt gctccagtga agacttcacc
     4981 caggttttac gcccggtatc agtaatcacc ggggcaagtt ccggcatctc attacacaac
     5041 agcacggggt caatcactcc tgaaacacgg gcctgtctgc agatgcctgt gtagatatcc
     5101 cgatacgctc gtacagaaaa aatgtccgcc ggtagtgtga tcagaatatc catcacttca
     5161 tgatctgccc cacgcagaaa gaatgcgcca atgacagcgc cttccaggtc atcattacgc
     5221 cagactggtg ttgtcatgca gccacacctc tgatacaaga acggtagctg ggccagttga
     5281 acgacaacca gttgcgcccc ccgtctgtga tcctgtcggc aatgcggggg ctgatgaac
//
LOCUS       DAJTVK010000195         5282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000195 DAJTVK010000000
VERSION     DAJTVK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.198"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..472)
                     /locus_tag="OO372_004025"
     CDS             complement(<1..472)
                     /locus_tag="OO372_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6343372.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(472..1392)
                     /locus_tag="OO372_004026"
     CDS             complement(472..1392)
                     /locus_tag="OO372_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6343373.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1530..2429
                     /gene="pgrR"
                     /locus_tag="OO372_004027"
     CDS             1530..2429
                     /gene="pgrR"
                     /locus_tag="OO372_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6343374.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2766..4379
                     /gene="mppA"
                     /locus_tag="OO372_004028"
     CDS             2766..4379
                     /gene="mppA"
                     /locus_tag="OO372_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6343375.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4430..>5282)
                     /gene="ynaI"
                     /locus_tag="OO372_004029"
     CDS             complement(4430..>5282)
                     /gene="ynaI"
                     /locus_tag="OO372_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6343376.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1429 a         1204 c         1226 g         1423 t
ORIGIN      
        1 cgccaatcgg atcgtaattg ctgaaacctt cccgaaaaag acgcccgata ttaacacccg
       61 taatactcac tactgcttta attcgttttt ctgtcagcgc ggcattaatg gcatagccac
      121 cacccccaca cactcccagc acgccaatac gttcagcatt aacatatggc agagttactg
      181 cataatcgat aacacggcta atatcctcta cgcgctgtgt cggatcttct atccagcgag
      241 gctccccacc agattctccc tggaaactgg cgtcataagc gagaacgaga tagccttttt
      301 cagccaatgc cttgccatag atattgccag acgtctgctc cttacaactg ccaaaaggat
      361 ggacgctgat aatcgccgga tactgttttg actcatcaaa atttggtgga aactgaatat
      421 tggcagcaat atgccaggcc atatcactat tgcggatttg aatatgtttc attacagagt
      481 ttcctcaaag aacggagcta atactgaaat tgcttcagcc acataggctt tgccgtcata
      541 aagatccata tggtttgcgc cttcaacaat gtgatagcgt ttatcttggc ttgatgctcg
      601 atcgtacagg tcgtcactca tccatttgct ccctgcctgg ctgcccgcca caatctgcat
      661 cggctgagtc aggtacactt ccgccatatg gtaagcatca aaggtaataa tctggttaag
      721 gctgcgcaaa gtagcgtaac ccggtgctgt tggatactgc gcgcgagggg tgtggtaata
      781 ctcccaggcc tgacgcagtt cttcgttcgg cgcatcggac tccttcattg gtgccagtgg
      841 cataacggca tattctccgc tgctaatatc gctggttcgg gcgtttgaac ccgcttcaac
      901 gtatggcagg gcatcaatag atttcacatt gttttcccaa ccattacgga acatcgaacc
      961 aatattgacc gcactaacgg taccgatggc cttgatgcgg cgatcctgaa ttgcagcatt
     1021 ggctgtatat cctgcaccgg cacaaattcc catcgcacca attcgggtat tgtcgacata
     1081 agaaagcgtt gtcaggtaat caatcacggc actgatgtct tcagtacgaa tgtatgggtt
     1141 ttctaactga cgcggctcgc cgccactttc accttgataa gatgcgtcat aagcaatagt
     1201 gacaaatccc ttttccgcca gttttttggc ataggttccg gccgtttgtt ctttaacgcc
     1261 cccacctggg tgagatacca caattgcctg atactgacgg gtttcatcaa attttggagg
     1321 gaaatagatc actgcagaca aagagatggt tggattattg ctgttagtga agctgacttt
     1381 attattcatc attccgttcc tcatggagtt gtcggttcgt tttaacggtt ggtgatatca
     1441 ctatagatat tgatcattaa gttgattaga cccaaaatta tgattagacc tatcatttaa
     1501 atgattaata gattgcatgg agatactgaa tgaagcgtga agaaatcgct gatctgatgg
     1561 cgtttgtcgt cgttgcagag gagcgtagct tcactcgtgc agcagcccgc ctgagcatgg
     1621 cgcagtcagc tttaagccag atagtgcgtc gtatagaaga acgattggga ttgcggcttc
     1681 tgacgcgaac cacgcgcagc gttgttccaa ctgaagcggg cgagcatctt ttgtctgttc
     1741 ttggccctat gttgcatgac atagattcag ccctggcatc cctgagcgag ctgcagaacc
     1801 gcccatccgg gacaatacgt attactactg tagaacatgc agccaaaacg atattgttac
     1861 cagcaatgcg cacattcctg aaatcgcatc ctgaaattga tattcagctc accattgatt
     1921 atggtttgac cgatgtcgtt tctgaacgtt ttgatgcagg cgtccgtctg ggtggggaga
     1981 tggataaaga tatgatcgcc attcgaatcg ggccagatat accaatggct attgttggct
     2041 caccggatta tttttctcgc cgaagtgttc caacgtcagt gtcacaatta atagatcatc
     2101 aggcaattaa tttgtatctt cccacatcgg gtacagcaaa tcgctggaga ttaatacgcg
     2161 gtggacgtga agttcgtgtt cgcatggaag gtcagctttt actgaatacg atagacctga
     2221 tcattgatgc tgcaattgat gggcatggat tggcgtatct accttatgat caggttgagc
     2281 gggctattaa agaaaaaaaa ctgatacgtg ttttggataa attcacacca gatttacccg
     2341 gttatcacct gtactatcca caccgtcgac atgctggctc ggcattctca ttatttatag
     2401 ataggctgaa gtataaaggt gctgtttagc actacttgct gatacattaa tttaattttt
     2461 ctcttaacgt attctcagtt cctttcaacg ttttggttat tttttattct tcgtacaatg
     2521 gcgacagatg ctgattatga taccgaaaac tggtttgaac gtgcgaagcc tgaacgtagt
     2581 gttcggtgtt ctatgtgctt taccgcattt tgtcgtctat tatttacact aaatctgatt
     2641 taatatattg atacataaaa cattttatgc tccctgtgtc acttttttga tggaagttgt
     2701 ttgcatttct ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga
     2761 aacttatgaa gcactctgtt tcagtcacgt gttgtgcgct gttggtcagc agcatttctc
     2821 tttcgtatgc tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc
     2881 gccacattaa agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga
     2941 ttcaggtcat tcgcgatctg tttgaaggac tggtgaatca gaacgagaaa ggggagattg
     3001 tccccggcgt tgcgactcag tggaaaagta atgacaaccg tatctggact tttaccctgc
     3061 gcgataacgc aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct
     3121 ggcagcgtct ggtggacccg aaaacgctat cgccatttgc atggtttgcc gcgctggcgg
     3181 gaatcaacaa cgcacaggcg attattgatg gtaaagctac gccagaccag cttggcgtca
     3241 ccgcagttga tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga
     3301 atttaaccgc taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag
     3361 agtggacgaa acccggaaat ctgatcggca atggcgctta tgttcttaaa gatcgcgtag
     3421 tcaatgaaaa actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtac
     3481 tgcaaaaagt gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacctcg
     3541 cgggggatat tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg
     3601 atattccggg gcaggtttat acgccgccgc agctcgggac ctattattat gcgtttaaca
     3661 cgcaaaaagg gccgacggca gatcagcgcg ttcgtctggc attaagtatg acgatagatc
     3721 gccgcctgat gaccgaaaaa gtattaggga cgggcgaaaa gccagcgtgg cattttacac
     3781 cagatgttac cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag
     3841 aactgaatgc gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc
     3901 tgaagctgac gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag
     3961 catcgatgtg gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa
     4021 cctatatcga tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtggggg
     4081 attataatga accctccact ttcctgacat tattaacgtc aacgcattca ggaaatattt
     4141 cacgctttaa caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg
     4201 ttaaagcgcg taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga
     4261 ttgcaccaat ttatcaatat accaatggac gattaatcaa gccgtggctg aaaggttatc
     4321 ccattaataa cccggaagat gtggcgtaca gccggactat gtatattgtg aagcattgat
     4381 gtgatgggaa ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgcccct
     4441 gttccggcgg tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc
     4501 cgtgtgactg cacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccatt
     4561 cagcccatac cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcggcaa
     4621 actggttgaa ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca
     4681 tctcacgtac ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa
     4741 tggtcgtggt aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa
     4801 acagcgagtt cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc
     4861 agccaatttc cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa
     4921 tactgaaagg acggtcgaaa tagagcatga tcccggaaaa gaaattactc agaatatctt
     4981 taccggccat accgacagcc agaccaccaa taccaccaaa ggtcagcaag ccagaaaggc
     5041 tcatgccgaa atgttcgcca taaagcagaa caagcaccac aataatggtg attttgataa
     5101 tgcgcgacat aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa
     5161 actgattaat cagcaaaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga
     5221 taaaatcgat aacgtctggg gtgataaatt tgagtttata gttttctata acataattaa
     5281 ta
//
LOCUS       DAJTVK010000196         5220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000196 DAJTVK010000000
VERSION     DAJTVK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.199"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..112
                     /locus_tag="OO372_004030"
     CDS             <1..112
                     /locus_tag="OO372_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6343377.1"
                     /translation="KASSEAVQMLISQGKTRLQIQEELGISRATYYRLAK"
     gene            481..1143
                     /locus_tag="OO372_004031"
     CDS             481..1143
                     /locus_tag="OO372_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6343378.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1507..2384
                     /locus_tag="OO372_004032"
                     /pseudo
     CDS             1507..2384
                     /locus_tag="OO372_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2384..2674
                     /locus_tag="OO372_004033"
     CDS             2384..2674
                     /locus_tag="OO372_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343379.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2995..3285
                     /locus_tag="OO372_004034"
     CDS             2995..3285
                     /locus_tag="OO372_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343380.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            3285..3704
                     /locus_tag="OO372_004035"
     CDS             3285..3704
                     /locus_tag="OO372_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343381.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            3872..4195
                     /locus_tag="OO372_004036"
     CDS             3872..4195
                     /locus_tag="OO372_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343382.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4625..>5220
                     /locus_tag="OO372_004037"
     CDS             4625..>5220
                     /locus_tag="OO372_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343383.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1718 a          941 c         1072 g         1489 t
ORIGIN      
        1 aaaagcttct tcagaggctg tacagatgct tatttcacaa ggtaaaacga ggttgcagat
       61 tcaggaagag ctaggaatct ctagagcaac ttattacaga cttgctaaat aagttactga
      121 atagttacat ggatgtagct gatatttggc ttttttgcca agggggtcac atagctggtg
      181 tgacgggtac taaacacgta tacatcatga ggtagattct accgaatcaa ctagacagat
      241 attttcgaag attcggggaa gatgagattc aaagagggct agtcattttt tataaaaatt
      301 ttcagactct gaaaagttac tgtacagaat atatacagat atctttaaag tatctgtaaa
      361 aacttttaag ataaaagata gatatcccag aaaggatatc cgtttagaaa acctgttaag
      421 catctcttaa aagagagtaa accttgtcaa gagtgattgc aataaaaatt tttatttctt
      481 atgtcaattg agcatatttt aatcgggaag catcatgggg gttctgatta ctggagaact
      541 ccatttttgt tatttaagaa cttacaccgt gaatttattt tcagtctaga tggtgctgca
      601 actgagcatg acacgctgtt accacgattc accgatgata taagccgtca atcatgggtt
      661 agtgaaaagg tcttctgtaa cccgccgtac agcgatattc cgtcgttcct cttaaaagcg
      721 tctgaagcgg atttagtagc tttcctgata ccgcatagag ccaacaccag ctactggctt
      781 aggcacattt acagcaataa ccactgtcat gaaatccgta ttcttcatcg tgcagttaaa
      841 taccttccac cagcagggca taacaggctt actattaggt caccgtttcc aagtgcagtc
      901 gttgttttta aaaaagaacc acgtaagcat gaaattacac agatggtttg ctgtgctgat
      961 acattgctac cgttaaccat cattaaccgt ggtggactaa gaggaagacc caccatttac
     1021 ccacctgaga cgctcgacag cttcataaaa ctatacaggc aaggaaaacc cattaagtac
     1081 attgcagatg ctctcagaat gcctctgagc acgtcataca ggattgctca aaggttgagc
     1141 taaaattctc ataaatatgg tcatttacaa gacaggtgat tcagatgtta agaagtatcc
     1201 ctgtgaacaa taaatgacca atcagtaatc attctgtgaa ataattgttg caatttataa
     1261 tcgcatctgt tatagtttct atatgggctg ataaaaaagt tcatgtcttg aagtggtaac
     1321 acttggggag gtgtcagatg gttggcgtat attaacctct cattcagggt atacctcagc
     1381 gttttaatca tcgtactgtt tcaggcatgg acttctttat cagtccatca atcccccaga
     1441 tattttaatc acacaggcta atcggtttta tccggttggc cttttcacgt atgaggtaat
     1501 ttatgaatgt ctaaatataa cccaaatcag ttttataaat ttgatgtgcg aatctctcaa
     1561 ctttcagaag aaattaacgc agcagcaaga ttgatttata tggtcatggt ccatagctgt
     1621 gacttctatg aagagaaata caaatcatat tcaccaagta ttgaccatct cgcttatatg
     1681 gctggtgtat caaagagaac agcaatcaga gccattgaga cacttgagaa acacggttta
     1741 attcaggtgc aaaagaatca ggggcgtcaa agcacttatc gtgtattcac actggatgat
     1801 aagccggaat tacttgtaag acctacatca caggaagttg cagcctcagt aagacaaaat
     1861 gacaccacta ccagtgacaa aatgtcatgg gggtagtgac aaaatgtcac ccgtgttaga
     1921 gtttagataa gttattagat aataatatta cagataagaa taaagagact ggacttaatg
     1981 atgatattaa agaagatgac tttctttctt tggatgaatt aaatgatttt ctctctatga
     2041 atgaatctga ccttgaagta agctctaaaa agacttctaa acaagacaga gaaatcactg
     2101 atgtgagtgt tgatagtgac cataaaaaca cacagtgccc cgccactgat gatgagttgg
     2161 accccttcga tgatgaagag gatgaacgtg aaaaagccat taagagggca gagaagaaaa
     2221 agagattcga gcttgcaaga caaaagaaca aagtatcggg tttagaccca ttcgatgaca
     2281 gtgaagatga ttattatctt tcaccgaaaa agaaacaaac tcaggatgac aagaagcaaa
     2341 aaattaaacc gaagatgtat tttgccggtg gaggaattaa gtaatgaaag aatatcacgg
     2401 tgaaaagaga tacaaagatt acctgttaaa gcgttacagc atttctcgtg aaggtcatct
     2461 tatgaaggat actcacgggg aagtttaccg gattagacca aagaaagaag ggaggggtta
     2521 tttcttttac gatggtgtga cagatttaaa gattgacgct ttgaggtttg cagtgctgta
     2581 tcattttgat atctggaatt caatccacca gctacgatta aaagacggtg accccagcaa
     2641 cttaaaggcc accaatatta tcacaaaacg ttagtttgtt ttaccatcat caatcgtaat
     2701 tgtgatttca tatttgaaaa gcttaatgct gaaattgaat cgtgtacttc tggcgataat
     2761 catttttaaa agctgataca caagaagcac cagaaacaca aagcaaccta agcttgcgaa
     2821 tctgtgagat tcagcaattg tttcaagaaa gcccattaat tccccttatt tatcaaaggg
     2881 aatgacctgt atcagccccc tacaaccata ttaacatgta aatgagttgt tgacaatatg
     2941 ttataaatat gttaaagtta gttataagtc aaagatttta agaaggaaaa tttaatgaga
     3001 cacacagtta cacgcctttt gccagaggca tattccagac tggcagcaat caaagacaaa
     3061 tacccagcca tgagccacac agcacttgtt ggtgagattt tgaaggaaca catagccctt
     3121 acaccgtttg acgctatcaa gggcgttgca gagggttcta aggccatcag aatcacaacg
     3181 ggccaactgg aactactcaa agcacagtcc gcagtggcag gttgctcaat gattcaggtg
     3241 ttgagtgcca aggttcatca actggcggat aagctggggg tttaatgcta tgcacatttg
     3301 attatcatgc gttttatcgc catagaggtc accttgtccg tgagaataac ggaacatgga
     3361 aagatttaac tcgtaatgct gaacccttca cacaccagca attaaaatcc cgcttctcca
     3421 gatggaagaa tgcaccagca gatactggtg attttgagat tatccatacc ttcgattatg
     3481 cctcagaaga tgaccttcac gatgcaatta agctgattga agacttcaat acctcagaag
     3541 atgtggatgg tgacaaatat aaatcccgtg gtaaccgcta taaccgtgat gatatcactc
     3601 aggccataaa tgctggtcaa tctcagcgat acattacaga aaaatatggt atttcacgga
     3661 gacagtattt ctacattaag ccattgatta ataaagacaa ttaaaaataa ccatctaaga
     3721 aacggtagaa tgcactttac aggcagagtt gcaccttaat ttgataaatt accttaccag
     3781 ccagtgcaac tcgctctgta acccgcatgg ttactggcct cagaatggct attttaagag
     3841 gtctgaggtg tgtttcatag gagatttaaa aatgaataat tacccaatgc aaattatggt
     3901 tgataacgac actgctatga tggtgcaaag cttcattgat gcaggtatcg caattaattt
     3961 tgataaatta ctaaagctga tggctgataa tgtagaaaac atctctgact ttattcagtc
     4021 ggtggagttt aacgagcctc gaatgatgct accaattaca gacagcaaca tgaaaagatt
     4081 ggttatcgag caaacgaaca tatacagtat ttccccagag caatttttga aaggtgctgt
     4141 gacaatcctg tacagtgata atattctggt gacggattct gtgagggttc actaatgggt
     4201 ttaataattg cttggacgtt gcttggctta tttgttggtt gtattattaa caatgcggta
     4261 aataagaatt gatgttatca acctgttaaa aagctgttga caaataacct taagcatggc
     4321 ataattttaa ttatgggaga gcaattctct cttatttttc tttcaataat ccgatggatg
     4381 gatattctga gttggaaagg tgcaatgctt ttcgtcatgg cctttcaggt tagtctgaga
     4441 ggctatctca aaaagtactc ttgttcattc attattgaat cctttgccct tcacagaaat
     4501 gtttagggct ttttcagttt ttaaaagcac acttggcagg ttcttgttca gtcagactct
     4561 tcacctctga ttgctgagtg tgcttctata aattgaaata acaaataaac taaggggtga
     4621 acgaatgatt agaaatgaac atgaaatgct tatcgaacac agaccaagtg cacttgatat
     4681 tcacgaatta actttgcgtg aatcttttga tagagcttgg cgggaatcac acgaagtaaa
     4741 taaaggtcca tatattccac cagaaccact cgagattcca agagttgaaa ttgatttttc
     4801 tatgaacgaa cggtgtgaac ttgatttgaa gttgaagcta caacgccgat atttccaagc
     4861 tattaaagac tctcaagaag caatgagaag tgtttacgag aaaattcagt ccaagcttga
     4921 agacgaaatt gacgaaatgg ttatttcgat ggagcttcaa accaattctt atgcttattt
     4981 ctctcgacta gaccgtgacg gttgggggta tgaccctgta gagattggta aagtgattgc
     5041 agatttacca gaaggtcaaa gagttgttcg tacagaaaga gctgtcagag caccacatcg
     5101 actgtctcta attgttgctg ataaatcaca agctgagctt gaagaaattg ccaaagagaa
     5161 actgatgaaa gcaagagaaa atgagattaa tttgctgaaa tcaagactgg ctaagctgat
//
LOCUS       DAJTVK010000197         5114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000197 DAJTVK010000000
VERSION     DAJTVK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.200"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..379
                     /locus_tag="OO372_004038"
     CDS             <1..379
                     /locus_tag="OO372_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343384.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="OO372_004039"
     CDS             complement(575..2125)
                     /locus_tag="OO372_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HCR6343385.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="OO372_004040"
     CDS             2297..2743
                     /locus_tag="OO372_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HCR6343386.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="OO372_004041"
     CDS             complement(2937..3872)
                     /locus_tag="OO372_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCR6343387.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="OO372_004042"
     CDS             4384..>5114
                     /locus_tag="OO372_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343388.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAJTVK010000198         5105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000198 DAJTVK010000000
VERSION     DAJTVK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.201"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            439..828
                     /locus_tag="OO372_004043"
     CDS             439..828
                     /locus_tag="OO372_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343389.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKY
                     CILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIH
                     SALNPTHYYQPVNDETTVMEIIKAYSK"
     gene            complement(892..1176)
                     /locus_tag="OO372_004044"
     CDS             complement(892..1176)
                     /locus_tag="OO372_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343390.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(1194..1391)
                     /locus_tag="OO372_004045"
     CDS             complement(1194..1391)
                     /locus_tag="OO372_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343391.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1603..1899
                     /locus_tag="OO372_004046"
     CDS             1603..1899
                     /locus_tag="OO372_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343392.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(2444..2677)
                     /locus_tag="OO372_004047"
     CDS             complement(2444..2677)
                     /locus_tag="OO372_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6343393.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2684..3214)
                     /locus_tag="OO372_004048"
     CDS             complement(2684..3214)
                     /locus_tag="OO372_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6343394.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(3192..3950)
                     /locus_tag="OO372_004049"
                     /pseudo
     CDS             complement(3192..3950)
                     /locus_tag="OO372_004049"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3892..4215)
                     /locus_tag="OO372_004050"
     CDS             complement(3892..4215)
                     /locus_tag="OO372_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6343395.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            4280..4471
                     /locus_tag="OO372_004051"
     CDS             4280..4471
                     /locus_tag="OO372_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6343396.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1347 a         1396 c         1088 g         1274 t
ORIGIN      
        1 ttaacgccac tggcagcagc cattggtaac taaaaagtgg atttagatac gcagaactct
       61 tgaagttgaa gccttatagc ggctacactg aaaggacatc ttttggtatc tgtgctccac
      121 ttaagccagc tacccggtta ggtaatctgc caggattagc taaccttcga aaaaccacct
      181 gccagggtgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag
      241 aagatccttt gatctttttt ctctaaactg caatcccatc agttcaggag agaaagatag
      301 tataattgcc ctctcttata tggaaccacc attcaatcac atcatgggga taatataagt
      361 gaaaacgttc gcattgttta tttttacgtg tatatgtatt tatagattgg aattttaaca
      421 aaataaaatg gagttaatat gttcaaatca tcagtaaagt caataacatc gagtactata
      481 ctgaccttac ttataggatg ttcaagtact gaaacaaaca aaaacattgt ttacaaagtt
      541 ggtggtgaca gcatcattct gaataaatat tgcatactta aaatgtaccc ttcccaatca
      601 gaaaaaaaca aaggcgtcat aatagaagta aacacagata gtttatgtaa gaaaaaacta
      661 aacgaaataa ctagtaaaaa tatagggaaa cccctgattt catattttga tggaaaagta
      721 atatcccaac ctgcaatcat tcattctgca ctaaatccaa cccattatta tcaacctgtg
      781 aacgatgaaa caacagtgat ggaaatcata aaagcatatt caaaataata ttaatagata
      841 taatcctgct taaggtatag cttaggcagg attatatcta ttcagctctt attataaggt
      901 agtgtataca tgaataatct gccctgtccc actgacactt ttaaatttaa ttttatcaac
      961 accagcgccc ttgcaagaga tctgataaga tgctggacca ataacgttaa ctgttcttgg
     1021 cccattaggg gcataccaga taaaagcaat aaatggaccg ggtacattag acaatgtatc
     1081 aaaatagaca gtgtacgcga tatcaccagt atgattttca agttcaaccc aaggaccaaa
     1141 aaagaaagga ggagcccaaa cattttgaga aatcatataa tctcctgatt agttcaatgc
     1201 gaatgttctt cccattcact ctcgcaagaa acatctataa gctcaacatc tagcccacta
     1261 acagaatcag agaaatactt ataaacatca tcccttgttc ccataaggtc tgccgaacat
     1321 gatagttcac aaccattctt attcctaact acaaataaga aacggtgaag caatactttt
     1381 tcttctttca ttttatccct ccctttattt acacagaata tactgcatat ataaacacta
     1441 gttttatata cagtataaat tacgccgcat tattttccct gtcaatgata tgaattttca
     1501 tttttgtgtt ctgaatcacc cttataaaaa tcaggaaggg aagattcgca gcagaaaaac
     1561 agcaccggat aacatcagga gaaaaaacag agaggagata acgtgagcaa aacaaaatct
     1621 ggtcgccacc gactgagcaa aacagacaaa cgcctgctgg ctgcacttat cgctgccgga
     1681 tacgaagagc ggacagcccg tgacctcatc cagaaacacg tttacacact gacacaggcc
     1741 gacctgcgcc atctggtcag tgaaatcagt aacggtgtgg gacagtcaca ggcctacgat
     1801 gcgatttacc aggcaagacg cattcgtctc gcccgtaaat tcctgaacgg aaaaaaaccg
     1861 gaagaagggc tgaacgcctg gaagggtgat ttaccataat cacttattgt acgcaccgct
     1921 gaaacgcgtt cggcgcgatc acggcagcag acaggtaaaa atggcaacaa accacccgaa
     1981 aaactgccgc gatcgcaccc ggtaaatttt aaccacatgc atagctatgc agccatgtga
     2041 atcatgcagg aattgccagc cagagacagc tgaaacggat ttttttcatg ctttcggaag
     2101 cgaagaagac ggggaccgga gcgggaaaac agaagttcag gggaatgagc gcgatctggc
     2161 aatagaggca aaacacagca acaaaagaca caccagaatc gcgcccgtat gcgttttaac
     2221 gcgttcctgt gttttcagca aggtctgact gaagcgcgtt cccggtgccc ccgaaccaac
     2281 cattatccca aaaaatgcac cggaaacgcg ctgacaacat tttagcctgt ctctgattac
     2341 catcccagcg aagggccatc cagtgtccgt tcctgtattt ccagctggcg ctcacgctcc
     2401 agggataacg catgttcgcg ttcctgtatc agccgttcgt cccttatctc ctgttctcgc
     2461 tgtataactg gctcaagcgt tctgtctgct cgctcaagtg ctgcacctgc tgactcaact
     2521 gcatgacccg ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac attttctgca
     2581 attccacgaa ggcgctctcc cattcgttca gccgctgcat atagtcctgt tgcagttgct
     2641 ctaatgcgtt cagcaaatgt ctttccagct ccgtcatgct ctgttactcc tgtaccagcc
     2701 ttacggcatt ctgtttcccg ttttccatcg tccagttcgt ttcggctttc atcccctcgg
     2761 gcaacaccag aaaacgtccg ttgctcccct cgtgataacg cacgccccat gtcttcgcgt
     2821 tcagcttctc cagattctcg ttctgcttcc tgatttcttc caggttcgcc tgtatcatcc
     2881 cgccaagata ccagagcgtc ccgccactca cggtaaacag gaaaatgacc atccccagta
     2941 acatcatgcc cgtattccct gccagcttta acacgtcctt cctgtgctgc atcatcgcct
     3001 ctttcacccc ttcccggtgt tttttcagtg attcctctgt cgaagctgtg aacagggcta
     3061 tagcgtcttt gattttcgtc tcgtttgatg tcacagcctt gcttacagat ttttcgagct
     3121 tcctgaactc gtcgttcagc attgtctctg tagattcggc tctctctttc agctttttct
     3181 cgaactccgc gcccgtctgt aaaagattgc tcatagaatg ctcctttcag tctgatattc
     3241 ttcccgccgt tcgggtctgc aatgctgaga ctgcttcgcg tcaccctgac cacttcaagc
     3301 cctgcctctg tgagcgcctg aatcacatcc tgacggcctt ttatctcccc gacatgataa
     3361 agagcgtcta tcccgcgtgt gacgctctct gcaagcgcct gtttcgtttc cggcaggtta
     3421 tcagggagag tcagtatccg gcggttctcc ggggcgttgg ggtcatgcag cccgtaatgg
     3481 tgattcacca gcgtctgcca ggcatcaatt ctcggcctgt ctgcccggtc gtagtacggc
     3541 tggaggcgtt ttccggtctg tagctccatg ttcggaatga caaaattcag ctcaagccgt
     3601 cccttgtcct ggtgctccac ccacaggatg ctgtactgat ttttttcgag accgggcatc
     3661 agtacacgct caaagctcgc catcactttt tcacgtcctc ccggtggcag ctccttctcc
     3721 gcgaacgaca gcacaccgga cgtgtatttc ttcgcaaatg gcgtggcatc gatgagttcc
     3781 cgtacttctt ccggattccc ctgaagcacc gttgcccctt cccggttacg ctcccttccc
     3841 agcaggtaat caaccggacc actgccacca ccttttcccc gggcatgaaa cttaactatc
     3901 atcccgcgtc ccctgttccc tgacggccag acgcagccgg cgcagctcat ccccgatggc
     3961 catcagtgcg gccaccacct gaacccggtc accggaagac cactgcccgc tgttcacctt
     4021 cctcgctgtc tgattcaggt tattcccgat ggcggccagc tgacgcagta acggcggtgc
     4081 cagtgtcggc agcctcccgg aacgggcaac cggctcaccc aagcagaccc gccgcatcca
     4141 taccgccagc tgtttaccct cacagcgttc cagtaaccgg gcatgttcat catcagtaac
     4201 ccgtatcgtg agcatcctct cgcgtttcat cggtatcatt acccccatga acagaaatcc
     4261 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
     4321 tatcagaagc cagacattaa cgctgctgga gaagctcaac gaactggacg cggatgaaca
     4381 ggcagatatc tgtgaatcgc ttcacgacca cgccgatgag ctttaccgca gttgcctcgc
     4441 tcgcttcggg gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
     4501 ttgtctgtga gcggatgccg ggagctgaca agcccgtcag ggcacgtcag cgggttttag
     4561 cgggtgtcgg ggcgcagccc tgacccagtc acgtagcgat agcggagtgt atactggctt
     4621 aaccatgcgc catcagtgcg gatggtatga aaagtgcgcc ataccgggtg tgaaatgccg
     4681 cacagatgcg taaggagaaa atgcacgtcc agccgctttt ccgcttcctc gctcactgac
     4741 tcgctacgct cggtcgttcg actgcggcga gcggtatcta ctcactcaaa aacgaaatgc
     4801 tgttatccac agaatcaggg gataacaccg gaaaaaacat gtgagcaaaa gaccaggaac
     4861 aggaagaagg ccgcgccgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gacttaaaga taccaggcgt
     4981 ttccccccgg aagctccctc gtgcgctctc ctgttccgac cttaccgttt accggatacc
     5041 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5101 tcagt
//
LOCUS       DAJTVK010000199         5044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000199 DAJTVK010000000
VERSION     DAJTVK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.202"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="OO372_004052"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="OO372_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="OO372_004053"
     CDS             1595..1903
                     /locus_tag="OO372_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6343397.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OO372_004054"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="OO372_004054"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6343398.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="OO372_004055"
     CDS             complement(3750..4700)
                     /locus_tag="OO372_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6343399.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="OO372_004056"
     CDS             complement(4839..>5044)
                     /locus_tag="OO372_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HCR6343400.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1229 g         1211 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttt aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAJTVK010000200         5041 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000200 DAJTVK010000000
VERSION     DAJTVK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.203"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="OO372_004057"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="OO372_004057"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(315..758)
                     /locus_tag="OO372_004058"
     CDS             complement(315..758)
                     /locus_tag="OO372_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6343401.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            915..1844
                     /gene="metA"
                     /locus_tag="OO372_004059"
     CDS             915..1844
                     /gene="metA"
                     /locus_tag="OO372_004059"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6343402.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2113..3714
                     /gene="aceB"
                     /locus_tag="OO372_004060"
                     /pseudo
     CDS             2113..3714
                     /gene="aceB"
                     /locus_tag="OO372_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3744..5035
                     /gene="aceA"
                     /locus_tag="OO372_004061"
                     /pseudo
     CDS             3744..5035
                     /gene="aceA"
                     /locus_tag="OO372_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1260 a         1320 c         1287 g         1174 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      241 attggcgcag aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc
      301 acggcagggg cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc
      361 aaatcgtcca cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc
      421 gcctgctcat tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc
      481 tccaccagca tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc
      541 atatgctgcc cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc
      601 cacaacggcg cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct
      661 gctgataaaa aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc
      721 ccttcctcat gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct
      781 tatattctaa cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat
      841 tggcaaattt tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt
      901 gaggtaatca ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc
      961 gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac
     1021 ttaaggttct gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc
     1081 gcctgctttc aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg
     1141 aatcgcgcaa cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc
     1201 aggaacaaaa ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta
     1261 atgatgtcgc ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca
     1321 cctcgacgct gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc
     1381 ctaagcaaac tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc
     1441 atgcgcttct gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg
     1501 actttccggc agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag
     1561 aaggggatgc atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc
     1621 ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag
     1681 acccggatgt accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga
     1741 gctggcgtag tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga
     1801 tcacgccata cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg
     1861 atcgttaagc gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa
     1921 acgcacctca gcaagtgaat aaattttatt catattgtta tcaataagtt atcaagtatt
     1981 tttaattaaa atggaaattg tttttgattt tgcattttaa atgagtagtc ttagttgtgc
     2041 tgaacgaaaa gcgcacaacg atccttcgtt cacagtgggg aagttttcgg atccatgacg
     2101 aggagctgca cgatgactga acaggcaaca acaaccgatg aactggcttt cacaaggccg
     2161 tatggcgagc aggagaagca aattcttact gccgaagcgg tagaatttct gactgagctg
     2221 gtgacgcatt ttacgccaca acgcaataaa cttctggcag cgcgcattca gcagcagcaa
     2281 gatattgata acggaacgtt gcctgatttt atttcggaaa cagcttccat tcgcgatgct
     2341 gattggaaaa ttcgcgggat tcctgcggac ttagaagacc gccgcgtaga gataactggc
     2401 ccggtagagc gcaagatggt gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc
     2461 gatttcgaag attcactggc cccggactgg aacaaagtga tcgacgggca aattaacctg
     2521 cgtgatgcag ttaacggcac catcagctat accaatgaag caggcaaaat ttatcagctc
     2581 aagcccaatc cagcggtttt gatttgtcgg gtacgcggtc tacacttgcc ggaaaaacat
     2641 gtcacctggc gtggtgaggc aatccccggt agcctgtttg attttgcgct ctatttcttc
     2701 cacaactatc aggcactgtt ggcaaagggc agcggtccct atttctatct gccgaaaacc
     2761 cagtcctggc aggaagcggc ctggtggagc gaagtcttca gctatgcaga agatcgcttt
     2821 aatctgccgc gcggcgccat caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc
     2881 cagatggacg aaatccttca cgcgctgcgc gaccatattg ttggtctgaa ctgcggtcgc
     2941 tgggattaca tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg tgtcctgcca
     3001 gacagacagg ccgtgacgat ggataaacca ttcctgaatg cttactcacg cctgctgatt
     3061 aaaacctgcc ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt tattccgagc
     3121 aaagatgaag agcgtaataa ccaggtgctc aacaaagtaa aagcggataa agcgctggaa
     3181 gtcaataacg gtcacgatgg cacatggatc gctcacccag gccttgcgga cacggcaatg
     3241 gcggtattca acgacattct cggctcccgt aaaaatcagc ttgaagtatt gcgcgaacaa
     3301 gacgcgccga ttactgccga tcagctgctg gcaccttgtg acggtgaacg caccgaagaa
     3361 ggtatgcgcg ccaacattcg cgtggctgtg cagtacatcg aagcgtggat ctccggcaac
     3421 ggctgcgtgc cgatttatgg cctgatggaa gatgcggcga cggctgaaat ttcccgtacc
     3481 tcgatctggc agtggatcca tcatcaaaaa acgttgagca atggcaaacc ggtgaccaaa
     3541 gccttgttcc gctagatgct gggcgaagag atgaaagtca ttgccagcga actgggcgaa
     3601 gaacgtttct cccagggccg ttttgacgat gccgcacgct tgatggaaca gatcaccact
     3661 tccgatgagt taattgattt cctgaccctg ccaggctacc gcctgttagc gtaaaccacc
     3721 acataactat ggagcatctg cacatgaaaa cccgtacaca acaaattgaa gaattacaga
     3781 aagagtggac tcaaccgcgt tgggaaggca ttactcgccc atacagtgcg gaagatgtgg
     3841 tgaaattacg cggttcagtc aatcctgaat gcacgctggc gcaactgggc gcagcgaaaa
     3901 tgtggcgtct gctgcacggt gagtcgaaaa aaggctacat caacagcctc ggtgcactga
     3961 ctggcggtca ggcgctgcaa caggcgaaag cgggtattga agcagtctat ctgtcgggat
     4021 ggcaggtagc ggcggacgct aacctggcgg ccagcatgta tccggatcag tcgctctatc
     4081 cggcaaactc ggtgccagct gtggtggagc ggatcaacaa caccttccgt cgtgccgatc
     4141 agatccaatg gtccgcgggc attgagccgg gcgatccgcg ctatgtcgat tacttcctgc
     4201 cgatcgttgc cgatgcggaa gccggttttg gcggtgtcct gaatgccttt gaactgatga
     4261 aagcgatgat tgaagccggt gcagcggcag ttcacttcga agatcagctg gcgtcagtga
     4321 agaaatgcgg tcacatgggc ggcaaagttt tggtgccaac tcaagaagct attcagaaac
     4381 tggtcgcggc gcgtctggca gctgacgtga cgggcgttcc aaccctactg gttgctcgta
     4441 ccgatgccga tgcagcggat ctgatcacct ccgattgcga cccgtatgac agcgaattta
     4501 ttaccggcga gcgtaccagt gaaggcttct tccgtactca tgcgggcatt gagcaagcga
     4561 tcagccgtgg cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa acctccacgc
     4621 cggatctgga actggcgcgt cgctttgcac aagctatcca cgcgaaatat ccgggcaaac
     4681 tgctggctta taactgctcg ccgtcgttca actggcagaa aaacctcgac gacaaaacta
     4741 ttgccagctt ccagcagcag ctgtcggata tgggctacaa gttccagttc atcaccctgg
     4801 caggtatcca cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat gcctagggcg
     4861 agggtatgaa gcactacgtt gagaaagtgc agcagccgga atttgccgct ataccttcgt
     4921 atctcaccag caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg
     4981 cggcacgtct tcagtcaccg cgctgaccgg ctccactgaa gaatcgcagt tctaagcaac
     5041 c
//
LOCUS       DAJTVK010000201         4804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000201 DAJTVK010000000
VERSION     DAJTVK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.204"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(279..1322)
                     /locus_tag="OO372_004062"
     CDS             complement(279..1322)
                     /locus_tag="OO372_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6343403.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1734..2918)
                     /locus_tag="OO372_004063"
     CDS             complement(1734..2918)
                     /locus_tag="OO372_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6343404.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(3218..3312)
                     /locus_tag="OO372_004064"
     tRNA            complement(3218..3312)
                     /locus_tag="OO372_004064"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3276..3278),aa:Sec,seq:tca)
     gene            3605..>4804
                     /locus_tag="OO372_004065"
     CDS             3605..>4804
                     /locus_tag="OO372_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR6343405.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1253 a         1056 c         1092 g         1403 t
ORIGIN      
        1 gggataccag ttcaaccgaa aacgccagag gaggggatta cccgctggca gggtaaatct
       61 gtggtttttg atgccagcta taacgtgttt gacgataaag tattacgaaa gaactttaat
      121 gagttcatgg agtaattcgt taaagtaatt tacaacgcta tccttttgca aaggaaactt
      181 tcagaaaata accccagggt aatcacctgg ggaaggaaca cagtgttatt attggataat
      241 aaattattac ttgttttaaa gttacactaa tgcaatatct actgatactg gatgcttgaa
      301 tgtttcatcc ctgctgcgga aaagggcaat atccagggcc agatatattc accgttcata
      361 tttctgcatt tctgtacaat ttcctcagaa agtgtcttgt caattctttt ccacaggtgc
      421 tcctgctgtc ttaaaatact ttcgcatatt tcgaaatcat cttcctgaat cagcatttcg
      481 gcgcatttaa tgatttctga tcgttcatgc agcacggagg aatggttata gagccagttt
      541 acaaacagca atgcttccgg atattgtccc cataatggtg agtatgttgc atagtaatct
      601 tctgccgtat ccagagagcc ggagaagtca tgccacacag caagtggaaa tggatgcaga
      661 tttccgcaca gcatttctgc gatgtaagtg tttgtggcaa agccatggcg aaaagctgtg
      721 gcggccttaa catgttcccc gttcagaaaa tgcaggagac cgagttcata ccagtaaggt
      781 gaaaattcat cagcatgctc tttcagtaca ctgaaggctc gctcatgatc gccggtgcgt
      841 aacagctctg aacccaacaa ccagcgagcc ccctgattat cttcaggatt gtatgccagt
      901 attttatcag tgagcataac cgcatcctga tggcgctgta agtgcacatt tgcgagaatt
      961 gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat gcatccagat tatttcacca
     1021 cagaaacttt ccgggatgat acgattacct gccgccagtc ctttaagaga ggcgttcagt
     1081 gccttacgcg gggcattctg ttccagaaat gcatatgcca gatgagcgtg tatatcaata
     1141 aagtccggct cccgttgagc aaggcgtcgg agtttgttga tgtaactcgt ctcgctgatt
     1201 tcatcagcgt cgtatgcatc aagcacttct tcaaactccc ccagcaatgg gccaaaccag
     1261 gtatccggaa atacgaaata acccttgtta tcgtgcactt caaagcataa tctatcgttc
     1321 atttctgagc ctgtaaaaca tgtggtgata tagtctgaga gcataaaagt tgagattctg
     1381 tgtatcaagt cctgtcttgg caccgaatga atattgctta cgaagcccct tcatgctttc
     1441 cttgagaaca ataattttat ctttatgtga atctcatttt ttataatgat gtcagatgat
     1501 tgaaatgcta aattatatct gaatcaactt ttcctttgct aaaggtatcg aaataactgc
     1561 aaggctgtat tttgtaattg ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg
     1621 taataagttt gaagacgcaa aaaaaataat ataataacaa aaatcctcca gccttgtagg
     1681 ccagaggata aaatacttaa ttgaggaaaa tatcagggaa aagtatttct gacttaactt
     1741 tcccggctgg agagtgaatc aatgtaatca gcgtaccatt gcatcatttc acggcggcca
     1801 tcgagataca gggcatgatt ataggtaccc ctgatggaat ttttatccac atgagctaac
     1861 tgcatttcaa tccaggcgga attaaagcct tgttcatgca gaattgtact catggtgtgt
     1921 ctgaaaccgt gccctgtgag tcttccctta tatccaagta attcaattac ttggttaatg
     1981 ctttccttac taattggttt gcggggatca tttctaccga taaagactag gggatgatgt
     2041 tttgagattg gttcaagctg cttaaatagc ataatgacct gctcagagag cggcacaata
     2101 tgaggtcgct tcatcttcat tacttctgcc gggatctccc acaattttgt ctcaaaatcg
     2161 atatcttccc agcgtgcaaa acgcagttct tgtgttcgta cgccggttaa cataatgatc
     2221 tgagtagctg tcttagtgat tatacttccg gtataacccg ccagatcgtt gaggaagtgg
     2281 ggaagttcat tagcagtaag aaagggaaaa tgtacttttt tcggtgtagc taaagcactg
     2341 gcaagatcgg gggctgggtt atagtctgcc cggccagtaa caattgcata gcggaacacc
     2401 tcaccacagc gctggcggac cttgcgcatt ttctccagag ctccacgttt ttccatttta
     2461 cgcagtgctt ccagtaactc cattggtttg atttcggcaa tagggcgctt gccaatgtag
     2521 ggaaaaatgt ctttttcaaa ggtatcaatg atttcatcac gatagcgtaa tgaccaccga
     2581 tcagcttttg actggtgcca ttcgcgagca atagcctcga acgtattttt cagactcatt
     2641 tgctgcgcga ttttttcttc cttttttgcc tcccctggat cgcctccagc agcgaggagt
     2701 ttcctggctt cactgcgttt ctcgcgagca tcggctagcg ttacatcggg gtatacgcca
     2761 atagaaagca tcttttcttt accagcaaag cggtacttca tgcgccagta tttagaacca
     2821 ttagtgttaa tcagtagata cataccgccg ccatcagcaa gcttataggg tttgtctttg
     2881 ggttttgcgg ttttcacttt gatatcggtt aatgccatga catcctccgg cttttgatga
     2941 attcatgttc ctgaggggga tttctgccag taactaacct gataccccca atacacccct
     3001 tgtataacaa agatattgtt aaaagccgtt gttatgtatc acatttttcc tattatgggg
     3061 gtgcttgtat ttcaatagga aataacaccc cctaaagtac ccccaaaaga aacttgatat
     3121 ggggtgaagt aggttgacct caggatacaa ataaatactg atatacaagg gattagaaga
     3181 gatttattga cattgggaga gttcagaaga ggtaaactgg cggaagatca caggagtcga
     3241 acctgcccgg gaccgctggc ggccccaact ggatttgaag tccagccgcc tcaccggaga
     3301 cgacgatctt ccgcgcctcg attgctacat ggaggcgggg cgcattatag ctacttcctt
     3361 gagtttctac atcccccaga tcagtttttt ccgcctaata cacgcagcgt attatcacga
     3421 aagcattata actcatataa aacccatatt ttacaatgat atatgagcgg aattaacgct
     3481 accacgatca cataaaacaa gaatcataaa ttaataacca gatatcggaa tattcgctct
     3541 cacagggatg gtttacaaaa tgcgttatcg gtctcagcac ccctatagca tcaaggaaaa
     3601 gcagatgaag agtgaagtgt tgtccgttaa agagaaaatt ggttatggca tgggagacgc
     3661 cgccagccac attattttcg ataacgtaat gttatatatg atgttctttt ataccgatat
     3721 ttttggcatt cctgccggat ttgtcggaac catgtttttg gtcgctcgtg cactggatgc
     3781 gatttccgat ccttgcatgg ggttgttggc cgatcgaacg cgctctcgct ggggtaaatt
     3841 tcgtccgtgg gtactgtttg gcgcactgcc attcgggatc gtctgtgtac tggcctatag
     3901 cacgccagat ctcagtatga acggcaaaat gatctatgca gcaattactt acaccctact
     3961 taccttactt tataccgtcg tcaatatccc ttactgcgca ttgggtggtg taatcaccaa
     4021 tgacccgact cagcgtatct cgctgcaatc ctggcgtttt gtgctggcga ccgcgggagg
     4081 catgctttct actgttctga tgatgccact ggttaattta attggcggtg ataataaacc
     4141 actcggtttc cagggcggta tcgcggtcct ttccgtggtg gcattcatga tgctggcatt
     4201 ttgtttcttc accactaaag aacgcgttga agcaccacct acaacaacgt ctatgcggga
     4261 agatttacgt gatatctggc aaaacgacca gtggcggatt gtcggtttac taaccatttt
     4321 caatatcctg gcggtgtgcg tacgcggtgg ggcgatgatg tattacgtca catggatttt
     4381 gggcacgccg gaagtgtttg tcgcttttct caccacttat tgcgtgggta acctgattgg
     4441 ttccgcactg gcaaaacctc tgaccgactg gaaatgtaaa gtcactatct tctggtggac
     4501 gaacgccctg ctggcagtga ttagcctcgc gatgttcttt gttcccatgc aggccagcat
     4561 cactatgttt gtcttcatct tcgtgattgg tgtgttgcat caactggtga cacctatcca
     4621 gtgggtaatg atgtccgata ccgtcgacta cggcgagtgg tgcaatggta aacgcctgac
     4681 cgggatcagt tttgctggca cgctgtttgt gctcaaactg gggttggcct tcggcggcgc
     4741 tcttatcggc tggatgctgg cttatggcgg atatgatgcg gcagaaaaag cgcagaacag
     4801 cgcc
//
LOCUS       DAJTVK010000202         4800 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000202 DAJTVK010000000
VERSION     DAJTVK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.205"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            242..583
                     /gene="raiA"
                     /locus_tag="OO372_004066"
     CDS             242..583
                     /gene="raiA"
                     /locus_tag="OO372_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6343406.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    686..811
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            687..734
                     /gene="pheL"
                     /locus_tag="OO372_004067"
     CDS             687..734
                     /gene="pheL"
                     /locus_tag="OO372_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR6343407.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="OO372_004068"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="OO372_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6343408.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO372_004069"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="OO372_004069"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6343409.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO372_004070"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="OO372_004070"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6343410.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..>4800
                     /locus_tag="OO372_004071"
     CDS             4448..>4800
                     /locus_tag="OO372_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6343411.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1311 a         1229 c         1096 g         1164 t
ORIGIN      
        1 ttgagctgcc gtttttttat tctgttagtt gtgaaactga agcgatttag tcactatcga
       61 tctcatcaaa tatggctcgc tttgagatat tcctcaagta aaaaaacacc tcttcctgcg
      121 atttctcaca aaaaagattc gttgacaaaa agtgacaaaa ttatgagatt cccatcacac
      181 attttgacat caggaacggt atgctgaatt caccaagacg ggaagacaag aggtaaaatt
      241 tatgacaatg aacattacca gcaaacaaat ggaaattact ccggccatcc gccaacatgt
      301 cgcagaccgt ctcgccaaac tggaaaaatg gcaaacacat ctgattaatc cacatatcat
      361 tctgtccaaa gagccacaag ggtttgttgc tgacgccaca atcaatacac ctaacggcgt
      421 tctggttgcc agtggtaaac atgaagatat gtacactgca attaacgaat tgatcaacaa
      481 gctggaacgg cagctcaata aactgcagca caaaggcgaa gcacgtcgtg ccgcaacatc
      541 ggtgaaagac gccaacttcg tcgaagaagt tgaagaagag tagtccttta tattgagtgg
      601 atcgccaacg cgccttcggg cgcgtttttt gttgacagcg tgaaaacagt acgggtactg
      661 tactaaagtc acttaaggaa acaaacatga aacacacacc gtttttcttc gcattctttt
      721 ttaccttccc ctgaatggga ggcgtttcgt cgtgtgaaac agaatgcgaa gacgaacaat
      781 aaggcctccc aaaccggggg gcctttttta ttgataacaa aaaaggcaac actatgacat
      841 cggaaaatcc attactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cattactggc agagcggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgagatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc agcaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cacgactgat ttatccacca
     1441 ttaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atcctcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcgaatc agcaacaaaa cttcacccga tttgtggtgt
     1681 tggcacgtaa agccattaac atgtctgacc aggttccggc gaaaacgacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgctggttg aagcgttgct ggtgctgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatatttt
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaattac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     2041 tggcggttgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat aatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag atgcagccca taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcag ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgaga gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatgacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcagc aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacaaacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttctttac acgaacaatt tgctcgcgct tttgatgctt
     4381 atttcgtaca aataaattta cacctccttt aaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttattggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcgggca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaaggacaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgta acaatgttga
     4741 aagtgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
//
LOCUS       DAJTVK010000203         4796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000203 DAJTVK010000000
VERSION     DAJTVK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.206"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..81
                     /locus_tag="OO372_004072"
     tRNA            6..81
                     /locus_tag="OO372_004072"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO372_004073"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO372_004073"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO372_004074"
     CDS             complement(299..1057)
                     /locus_tag="OO372_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6343412.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO372_004075"
     CDS             complement(1065..2168)
                     /locus_tag="OO372_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6343413.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="OO372_004076"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="OO372_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="OO372_004077"
     CDS             complement(3427..4452)
                     /locus_tag="OO372_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6343414.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAJTVK010000204         4700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000204 DAJTVK010000000
VERSION     DAJTVK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.207"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            387..1469
                     /locus_tag="OO372_004078"
     CDS             387..1469
                     /locus_tag="OO372_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6343415.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OO372_004079"
     CDS             complement(1658..2041)
                     /gene="quuD"
                     /locus_tag="OO372_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCR6343416.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2127..2267)
                     /locus_tag="OO372_004080"
     CDS             complement(2127..2267)
                     /locus_tag="OO372_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HCR6343417.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OO372_004081"
     CDS             complement(2264..2626)
                     /gene="rusA"
                     /locus_tag="OO372_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HCR6343418.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2623..2913)
                     /locus_tag="OO372_004082"
     CDS             complement(2623..2913)
                     /locus_tag="OO372_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6343419.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2906..3076)
                     /locus_tag="OO372_004083"
     CDS             complement(2906..3076)
                     /locus_tag="OO372_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343420.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(3076..3531)
                     /locus_tag="OO372_004084"
     CDS             complement(3076..3531)
                     /locus_tag="OO372_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HCR6343421.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3796..4173
                     /locus_tag="OO372_004085"
     CDS             3796..4173
                     /locus_tag="OO372_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343422.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
BASE COUNT         1351 a         1048 c          978 g         1323 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaaccttg
     1561 cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg cgttgcaacg
     1621 ccccattttt aataattttt agtaaacaat tggcatatta attagagtta ttaacaacga
     1681 tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg ccctcagcct
     1741 tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc agtgacatga
     1801 atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg ttgttctttt
     1861 tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat tgcgggcgag
     1921 attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac caggtcacat
     1981 cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga atatcacgca
     2041 tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat caacaaaaca
     2101 gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt tttggcgtaa
     2161 ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa gcgcagatgc
     2221 ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca ttccccattt
     2281 cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca acgcggtaat
     2341 caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca aaagcggctt
     2401 tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca atacggattt
     2461 tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg gtgacgttat
     2521 cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg cggtaatagc
     2581 ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct taataacccc
     2641 ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt cttttgcata
     2701 ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag aacatgcaat
     2761 ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca gccggatatg
     2821 tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta cctggcattc
     2881 ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc agtcgcaacc
     2941 attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg gatggcttca
     3001 gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc atgacacgaa
     3061 taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt cacagctctg
     3121 cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc acaggaagat
     3181 gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg tggtgacctc
     3241 gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt gcttcatcgc
     3301 gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat attcgctgat
     3361 ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct cgcgccacgg
     3421 cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct gctggccgat
     3481 ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca tacggcctcc
     3541 ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg tgacaaggtg
     3601 atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc accggagttg
     3661 ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa ctatcttgaa
     3721 gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc acagccagaa
     3781 ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa caagttcaag
     3841 aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt atttaactct
     3901 aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag aaaatcatct
     3961 attgctggta attttactaa attaggtgag gcaattacct cgagtgggca gagggttttt
     4021 ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga tggagataca
     4081 atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc ttccaaagac
     4141 gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag gatgcactca
     4201 agtcagtggt gaagtaagag atctagtaat tcttcttgaa actgatggaa aaacggtgtc
     4261 tgattatttg cagccaatcc ctcccaaaaa agatacacac tggagcatga ttttcattcc
     4321 ggtagcaatt tatatactac ttaatcttgt atacattatt gctcacccat tatttcctga
     4381 aaaatatcta aaaacatcaa catctatatt tctattcatt tccttcatat taagtgcaat
     4441 agtagcatat tttgttttcc ataagcataa atctgccact ctatccatat gcagcttagt
     4501 gttcttagtg ttaattgtag gtgctaacgt agggtttatc agttacagcg acctaacaca
     4561 aaaagcgacg caaaaattag atagtttgtc taaataatta tgttgccatg cgaagtatgc
     4621 atggctgcat gtctgccttc catttaaaat gggctaagac ctataaccct aaatattatt
     4681 ctttattatc ttctttacca
//
LOCUS       DAJTVK010000205         4607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000205 DAJTVK010000000
VERSION     DAJTVK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.208"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..2162
                     /locus_tag="OO372_004086"
     CDS             <1..2162
                     /locus_tag="OO372_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6343423.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="OO372_004087"
     CDS             complement(2396..3679)
                     /locus_tag="OO372_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6343424.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..4506)
                     /locus_tag="OO372_004088"
     CDS             complement(3814..4506)
                     /locus_tag="OO372_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6343425.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1178 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgct
//
LOCUS       DAJTVK010000206         4563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000206 DAJTVK010000000
VERSION     DAJTVK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.209"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..827)
                     /locus_tag="OO372_004089"
     CDS             complement(<1..827)
                     /locus_tag="OO372_004089"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6343426.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     "
     gene            959..2068
                     /gene="apbC"
                     /locus_tag="OO372_004090"
     CDS             959..2068
                     /gene="apbC"
                     /locus_tag="OO372_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6343427.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            2313..2594
                     /locus_tag="OO372_004091"
     CDS             2313..2594
                     /locus_tag="OO372_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR6343428.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            2837..3379
                     /locus_tag="OO372_004092"
     CDS             2837..3379
                     /locus_tag="OO372_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6343429.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            3459..4133
                     /locus_tag="OO372_004093"
     CDS             3459..4133
                     /locus_tag="OO372_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6343430.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            4149..>4563
                     /locus_tag="OO372_004094"
     CDS             4149..>4563
                     /locus_tag="OO372_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6343431.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1267 a          977 c         1085 g         1234 t
ORIGIN      
        1 acgctgtcgc tgcgcttgtc gcacagattc ttgaaagaac ccatgtagcc aatcggtgcg
       61 tccagccaga cgtagaaata tttgcccggc gcgttcggaa tttcaaaacc aaagtatggc
      121 gcgtcgcggg agatatccca ctgttgcagg ccggattcaa accactcctg cattttattt
      181 gccacctgct cctgcaacgc accgctgcgg gtccatgcct gcaacatttc gctgaaagag
      241 ggcagatcaa agaagaagtg ttcagaatca cgcattaccg gcgtagcgcc agaaaccacc
      301 gatttcggct cgatcaattc agtcgggctg taggtcgcgc cgcagacttc gcagttatcg
      361 ccgtattgat ccggggattt acatttcggg caggtgcctt tcacaaaacg gtccggcagg
      421 aacatgcctt tttccggatc gtacagctga gagatggtgc ggtttttaat aaaaccgttc
      481 tctttcaggc gagagtagat aagttctgac aactggcggt tctcttcgct gtgcgtcgag
      541 tgatagttgt catagctgat gttaaagcct gcgaaatcag tctgatgctc ctgactcatt
      601 tcgccaatca tctgctccgg ggtgatacca agctgctgag ctttcagcat gatcggtgta
      661 ccgtgggcat cgtcggcgca gatgaagttg acctcgtggc cgcgcattcg ctggtaacgg
      721 acccagacat cagcctggat gtgctccagc atatggccga ggtggattga gccgttagcg
      781 tacggcagtg cgcacgtcac cagaattttc ttcgcgactt gagtcatagt aggcattact
      841 tcttaatagt gaaaaggggt tagatagtac caaatgggaa aatgttaagt aagtacttct
      901 ctatattctt caggtaaact atcagctgat ggtttatcta tacaaaaaag gagtcgggat
      961 gaacgaacaa tcccaggcca aatcaccaga agccttgcgc gcaatggtcg ccgggacact
     1021 ggccaatttt cagcacccaa ccctgaagca taacctcacc acgctgaacg cgttgcacca
     1081 tgtcgcctgg atggacgaca cgttgcatgt ggaactggtt atgccattcg tctggcatag
     1141 cgcgtttgaa gagttaaaag agcaatgtag cgccgaactg ctgcgtatca ccggggcgaa
     1201 agctatcgac tggaaactgt cgcacaatat cgccacctta aaacgcgtga agaatcagcc
     1261 gggtattaat ggtgtgaaga acatcattgc ggtcagttcc ggtaaaggcg gcgtgggtaa
     1321 atcctccacg gcggtaaacc tggcgctggc actggcggcg gaaggggcga aagtcggcat
     1381 tctggacgcc gatatctacg gcccatcaat tcccaccatg ctgggcgcag aaaatcagcg
     1441 tccaacttcg ccggacggta cccatatggc accgattatg tctcatggcc tggcaaccaa
     1501 ttcaattggt tatctggtca ctgacgacaa tgcgatggtg tggcgtggac cgatggcgag
     1561 caaggcgtta atgcagatgt tgcaggaaac cttgtggccg gatctcgact atctggtgct
     1621 tgatatgccg ccaggcaccg gtgacattca attgacgctg gcgcaaaaca ttccagtaac
     1681 tggtgcagtc gtggtcacca ccccgcagga catcgctttg atcgatgcga aaaaaggcat
     1741 tgtgatgttc gagaaagtcg aggttccggt gcttggtatt gtcgagaaca tgagtgtgca
     1801 tatttgcagt aactgtggtc accatgaacc gattttcggt accggtggtg cagagaaact
     1861 ggctgaaaaa taccacacac agctgctggg gcagatgccg ttacacatct ccttacgtga
     1921 ggatctcgat aaaggcacgc caacggttat cagccgtccg gagagcgaat ttaccgctat
     1981 ctaccgccag ctggctgacc gcgttgcagc tcagctctac tggcagggtg aagtcattcc
     2041 aggcgagatt tccttccgcg cggtctaacg cctattaacc attctggtta ttcttcatac
     2101 accgcagaga tgttacattg atgcggtgta ttagtttttg ccgcattaat gaacacttat
     2161 ttaatactgc gaatattctg cggaccttcg tccgaaactc tacaaagtcg attgatcata
     2221 caggcaatgc ttcattatca tccttcgtgg ctgtgtagtg gatgtacagc tatagtttgt
     2281 tatgcactca ttctatccag gcataacctt caatgaataa gtattggctg tcaggaatta
     2341 tttttcttgc ctatggattg gcttctcccg cattctcttc agagactgcc acattagcga
     2401 ttaatggcag gatcagtccc ccaacatgca gtatggctat ggttaatggt caacctcaac
     2461 cgcattgtgg tcagttaacg tataatgttg acacccgtca tctgttttct tcgcccgtca
     2521 aaggtgtaac gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa
     2581 atcgctacga ttaatttggc aacctgattt ttattgatat caacaatacc atttacataa
     2641 atctacatta attgatagat gcgataaata gttaatactc ttaaatattt gttgtattta
     2701 tattgcaatg gttaatttgt ttcaaataaa taaaacaatg tgtttacatt gcggtaactt
     2761 ttttgatgtg agaaatttgt ttccaagaga tacagactct taacaaccaa aaaaaaattt
     2821 tattaaggaa catattatga aacgttcaat tattgctgcc gctgtctttt cttctttttt
     2881 tatgagcgct ggagtatttg ctgcagacgt tgataccgga acattaacta ttaaggggaa
     2941 tattgcagaa tctccgtgta aattcgaagc gggtggtgat tcagtaagta ttaatatgcc
     3001 gactgtacca accactgtct ttgaaggtaa agctaaatat tctacctatg atgatgcagt
     3061 cggtgtaacc agcagcatgt taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact
     3121 ctcgttgatt accaacgata aaataaccgg taacgataag gcgatagcca gtagcaacga
     3181 taccgtgggt tactatctct atttaggtga taacagcgat gtcctggatg tttctgcacc
     3241 ttttaacatt gagagttata aaacagcgga aggtcaatat gctattccgt ttaaagcaaa
     3301 atacctgaaa ctgacagata actcagtgca atcaggtgat gtgttatctt ctctggttat
     3361 gcgtgtggcg caggattaat aaataaaatt gtaagtttct ccaggtaata aacatggccg
     3421 ctatcccatg gcggcctttt aatttaagag gcattaaaat gaaaggatta ttatctttac
     3481 tcattttttc tatggtcctt cctgcacatg ccggaattgt tatctacggg acgcgcatta
     3541 tttacccggc agaaaataaa gaagtgatgg tgcagttgat gaaccaggga aaccgttctt
     3601 cgctgctgca ggcgtggatt gatgatggcg atacgtcatt accaccagaa aaaattcagg
     3661 ttcctttcat gttaacgcca ccagtggcaa aaataggggc aaattccggg cagcaagtaa
     3721 aaatcaaaat tatgccgaat aaactgccca ctaataaaga aagcattttt tatctgaatg
     3781 ttctggatat tccaccaaat agtccagagc aagaaggtaa gaatgcactg aagtttgcga
     3841 tgcaaaacag aattaagttg ttttaccgac cagcgggtat tgctccggta aataaagcga
     3901 catttaaaaa attgctggta aatcgcagtg gcaatggttt ggtgataaaa aatgactcag
     3961 ctaattgggt gacgatttcg gatgtcaaag ctaataatgt caaagtcaat tatgaaacta
     4021 ttatgattgc ccccttagaa agtcagagtg ttaatgtcaa aagtaataat gcaaataact
     4081 ggcatctgac cattatcgat gaccatggca actatattag tgacaaaatt taattgcagg
     4141 agctgcctat gttgagaatg accccacttg catcagcaat cgtagcgtta ttgctcggca
     4201 ttgaagctta tgcagctgaa gaaacctttg atacccattt tatgataggt ggaatgaaag
     4261 accagcaggt tgcaaatatt cgtcttgatg ataatcaacc cttaccgggg cagtatgaca
     4321 tcgatattta tgtcaataag caatggcgcg ggaaatatga gattattgtt aaagacaacc
     4381 cgcaagaaac atgtttatca agagaagtta tcaagcggtt aggcattaat agcgataact
     4441 tcgccagcgg taagcaatgt ttaacatttg agcaacttgt tcagggtggg agctatacct
     4501 gggatatcgg ggtttttcgt ctcgatttca gtgtcccgca ggcctgggtg gaagaactgg
     4561 aaa
//
LOCUS       DAJTVK010000207         4483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000207 DAJTVK010000000
VERSION     DAJTVK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.210"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(8..1069)
                     /locus_tag="OO372_004095"
     CDS             complement(8..1069)
                     /locus_tag="OO372_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6343432.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="OO372_004096"
     CDS             complement(1066..2463)
                     /locus_tag="OO372_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6343433.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="OO372_004097"
     CDS             2618..3616
                     /locus_tag="OO372_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6343434.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="OO372_004098"
     CDS             complement(3727..>4483)
                     /gene="ompG"
                     /locus_tag="OO372_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCR6343435.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1064 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaattgatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc gcc
//
LOCUS       DAJTVK010000208         4475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000208 DAJTVK010000000
VERSION     DAJTVK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.211"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..1133)
                     /locus_tag="OO372_004099"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="OO372_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="OO372_004100"
     CDS             complement(1133..1315)
                     /locus_tag="OO372_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343436.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="OO372_004101"
     CDS             complement(1327..3060)
                     /locus_tag="OO372_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6343437.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="OO372_004102"
     CDS             complement(3057..3551)
                     /locus_tag="OO372_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6343438.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="OO372_004103"
     CDS             complement(3673..4023)
                     /locus_tag="OO372_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6343439.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..>4475)
                     /locus_tag="OO372_004104"
     CDS             complement(4066..>4475)
                     /locus_tag="OO372_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6343440.1"
                     /translation="SWAVNHYRDNAITYKEQRDKNARELKLANATITDMQQRQRDVNA
                     LDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSVREATTASGVDNATSPRLAD
                     TAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT          962 a         1325 c         1125 g         1063 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgaca
//
LOCUS       DAJTVK010000209         4409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000209 DAJTVK010000000
VERSION     DAJTVK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.212"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(173..2122)
                     /locus_tag="OO372_004105"
     CDS             complement(173..2122)
                     /locus_tag="OO372_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6343441.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="OO372_004106"
     CDS             complement(2207..2542)
                     /locus_tag="OO372_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6343442.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="OO372_004107"
     CDS             complement(2542..2898)
                     /locus_tag="OO372_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6343443.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="OO372_004108"
     CDS             complement(2898..4394)
                     /locus_tag="OO372_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6343444.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1314 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAJTVK010000210         4404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000210 DAJTVK010000000
VERSION     DAJTVK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.213"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(139..3654)
                     /locus_tag="OO372_004109"
     CDS             complement(139..3654)
                     /locus_tag="OO372_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6343445.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="OO372_004110"
     CDS             complement(4106..>4404)
                     /locus_tag="OO372_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6343446.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1128 c          905 g         1230 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAJTVK010000211         4402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000211 DAJTVK010000000
VERSION     DAJTVK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.214"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..590)
                     /locus_tag="OO372_004111"
     CDS             complement(<1..590)
                     /locus_tag="OO372_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6343447.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="OO372_004112"
     CDS             complement(583..993)
                     /locus_tag="OO372_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR6343448.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="OO372_004113"
     CDS             complement(999..1532)
                     /locus_tag="OO372_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR6343449.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2592)
                     /locus_tag="OO372_004114"
     CDS             complement(1510..2592)
                     /locus_tag="OO372_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR6343450.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RNYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2589..3980)
                     /locus_tag="OO372_004115"
     CDS             complement(2589..3980)
                     /locus_tag="OO372_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR6343451.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4027..4233)
                     /locus_tag="OO372_004116"
     CDS             complement(4027..4233)
                     /locus_tag="OO372_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343452.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1054 a         1282 c         1040 g         1026 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcgccgtc gccgctttac ctgttccggg ttttcccaga accagtcgcc cgcattcatc
     2101 gtcataagcc agcgtctgaa cctgccccag taaccggtta agacaatcgg caacggtttc
     2161 gccgtgttcc ggctgggcat caatcaccgc tgtctgcggc acgccagcat caacaacggt
     2221 gatgccaaat ggcgcagcca gttcactgac tattctgagc aggttttttc cgctctgctg
     2281 gagtggcaaa gcagagcagt caaccagatc ggctgttttg cttcgcccga caattcccat
     2341 gctgacgctg ctggcgtcat aacgaagcgg tagtgcctcc acatatccgg tgagcacagg
     2401 ctcatccccg ataagcactt caaccagctc accatttttt atccggggtt gataattccg
     2461 gcttccgggc cagcgggtgg taatggcaac attaaaatcc cgggcaatac ggttaatgcc
     2521 cgcactgata cggacggatg tccagccgcc ccattcgcga ccggaaaccc gaagtaaaac
     2581 ggtatcattc atctgacggg tacccttaat gccctgaccg gaacaaagcc cggatgagag
     2641 atggcatttc gatccaggat atcagtttca cgggacgcat cgtcgtacca tgacgcagcc
     2701 agaacaagcg caggcagaac ttccgctggc gttcgctctg cggtttcctc cgtctgaacc
     2761 agacgcgcct gaatatcccg gttcagttct gtacgtaatg acgtcagctg aaaaaacagc
     2821 ctgtcatccg ccgtacgcct gagctcctgt tcaatcgccg cgttcagcga tttgcgtatg
     2881 atggtgagat tttcccgcgt gggtggttgc gctctctcat cctgtcctgt gctggctgcc
     2941 acgctgtcaa gtgccggatg agaaacgtga ataatatcgg actgacgttc agtggaacca
     3001 ccaacagcca caaccgcctg ctgatttttc accaggcttc cgggttgtgg cagtgaagtg
     3061 accgcccttg tggcttcgct gattgccgtc gtccggatga cagcagccac aaggtttgtc
     3121 tgctgttttt gcctgataac cgatgcggaa tctgtgggcc acaccgcacg cggtgccagt
     3181 cctgggtcca gcgtaatgcc ggacattgtc gttatggact gcaccagatc ctgtgtgtta
     3241 tccaccagcc gggttccggc ccgccaggta tcctgcaacg tatgcacaaa atcactggca
     3301 accgacggcg gcatcagaat gacggataaa tcgccctgca gtagtctcgc tccggcagaa
     3361 acagccgaat taaccatttt gaaagctgtc tgaacggttc ccagcatatc ggtcgtccgg
     3421 gcaataacat cgttctgaat aaagtccggt attcccgcga gatcaaaatc gccgaacatg
     3481 tcttcaatca actcatccag gaatacggac gactcctcca gttttctggc ggttgcagct
     3541 ccggcgaccg gaaatgacag ttccccgctc tcaacaaact gaaatgaaac ccggcacata
     3601 cggccttcag tgccggagtg agaaacggtc acctgcccgt caatacagcc ctgcatttca
     3661 ccgaactgcg gatggatcag cgtccccggc ccggcggttt caatcgctgt aatcagcctg
     3721 tcgcgctgct ctgcgtaatc atcaccaacg agatacgcat taatcgtcag ccgtcgcgtg
     3781 gcacgcccga gatcttccgt gtacggtttg tcacggtttg ggtattcatg gacctgaacg
     3841 cggcgtccga acgaaccctc gtcgctctcc accgaaaacg gaacgccacg aaatgaagcg
     3901 tcgtaaagat tatcgcgcca attgatacct gaagatgcag aaaagagtga agaaagagaa
     3961 ggtaaagaag gtaagtccat actgtctcca ctataaaaaa accgccaatt tctggcggct
     4021 tatatattaa tcatcattaa tatctttcat attcaatttg caattttcgt aggtcactcc
     4081 cgactcgcca ttaacattaa ctccagctct gatattcccg ttttctttta catctacaaa
     4141 aaactggcct cttggcatct tgaacgcaaa tgtaaaacca acagtatctg aatcaaaatc
     4201 atcatcttta tatattggga tcaaaaatgt catcgtcgct ggtgatgcat tgtttgcaaa
     4261 atcaaataca cctatacttt tgccgaattc ataagaagct tttctcaata taatcgcacc
     4321 gctggcagaa ggggatgggc acgatatcct cagttgtctg aatccatctg tatccaagtc
     4381 aacgtcatta tctgcagatg tt
//
LOCUS       DAJTVK010000212         4398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000212 DAJTVK010000000
VERSION     DAJTVK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.215"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(10..1947)
                     /locus_tag="OO372_004117"
     CDS             complement(10..1947)
                     /locus_tag="OO372_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6343453.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2358..3065
                     /locus_tag="OO372_004118"
     CDS             2358..3065
                     /locus_tag="OO372_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6343454.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3171..3246
                     /locus_tag="OO372_004119"
     tRNA            3171..3246
                     /locus_tag="OO372_004119"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3204..3206,aa:Phe,seq:gaa)
     gene            3444..>4398
                     /locus_tag="OO372_004120"
     CDS             3444..>4398
                     /locus_tag="OO372_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6343455.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1147 a         1160 c         1055 g         1036 t
ORIGIN      
        1 acgtcgccct cagggaatta aacctaaaaa gaaaatcgtc aaaattagag attccggttt
       61 gacacggaaa gtcatatcag cgctatgcgc atcctggggg ttcatcacca ctgacgggaa
      121 actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga catacagatc
      181 gtgcatctcc tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg gatatttgtt
      241 ataaacgctc ggcaacacct cagccagcgg cgagtcatcc tcaatatgct gttcaaattg
      301 cgccagcatc gccaccagtt gtgccagctt ctcgtggctt tccgccggag ttaataagaa
      361 cagaatggag ttgagatcgc acttctccgg cacaatgccg ttctcacgca gatagtgcgc
      421 cagaatcgtc gccggaacgc caaagtcgct atattcgccg gtttcggcat tgatacctgg
      481 tgtagtgagt aacagcttgc acggatcaac aaaatactga tccgcggcat atccttcaaa
      541 gccgtgccac ttcgcccccg gctcaaaact gaaaaaacgg cggtcgctgg ctaacacaga
      601 tgtcggataa tcctgccaca atttgccatc aacaacgggc gggataaacg ggcggaacag
      661 cttacagcgc gcaagaatag ccttgcgcga ctcaataccc aactcaacgc actcagccca
      721 cagccgacgc ccactctccc cttcatgaat tttggcgtta acatccagtg cagcaaacag
      781 cggatagaaa gggctggtag aagcatggag cataaaggcg ttattcaacc gcttatgcgg
      841 gcaaaaacgc gcctgtccgc ggatatggtt atctttttta tggatctgcg acgtctgtga
      901 gaatcccgcc tgctgtttgt gcaccgactg agtcacaaag atccccggat cgttttcgtt
      961 aagttctaat agcagcggcg agctatccgc catcatcggg ataaactgtt cgtaaccgac
     1021 ccacgcggaa tcaaacagaa tgtaatcaca cagatgccca acggtatcga tcacctgacg
     1081 ggcgttatag acagttccgt cataggttcc cagctgaata atcgccaggc gaaacgggcg
     1141 cggcagctcg gctttttctg gcgcaacgtc gcgaatttgc tggcgcagat actcttcatt
     1201 aaaacagtgc gcatcaatac cgccaatgaa accaaacggg ttgcgtgaag cttccagata
     1261 gaccgacgtc gcccccgcct gaatcagcgc gccgtgatga ttcgacttat ggttgttacg
     1321 gtcgaagagc accagatcgc cacgcgttaa cagcgcattc gtcaccactt tattcgctgc
     1381 cgatgtgccg ttcagcacaa aataggtttt atcggcatga aagactttgg ctgcgaattt
     1441 ctgcgcatcc ttcgccgatc cttcatgaat aagcagatcg cccaatttta cgtcagcgtt
     1501 acacatatcg gcgcgaaaga cgttctcacc aaagaaatcg taaaaatggc gtccggcagg
     1561 atgcttttta aaaaacgcac catgttgatg tccagggcaa gcaaaggtgc tgttgcccat
     1621 ctcaacgtac tgcgtcagcg tgtcataaaa cggtggcagc aaattctctt catactgaca
     1681 ggctgcggat tccagctcca gccactgctg ctcgttgccg ttgattaccg ccgtaacgcc
     1741 cgcaggtaat tcaacagcat gttcggaata caaaaacacc ggtagatgaa aaccggtgcg
     1801 cttaagcaac gcaagaatgc cactgcgact atccgcagcg gtaatgacga ctgccgcgac
     1861 atccgtaaaa tcagtatctc ccaacgccac cacgcggcga tgagtagaaa gtcgggatac
     1921 cagttcacta ctggcggcaa tattcattga tttcataagc gcaaaacccg tttcggggaa
     1981 gtaagaatac cggacaaagt gtaaaaccct gccccatgag atatgggtca aactggtcac
     2041 cagctccgac gaccgccaga catcagtaaa agcagaaacg ctctgatttt actgttgtcc
     2101 tgcagtgaac gtgcgttaac ttcaccgcat gagcagtaac atagaaaggg aaacgtttcg
     2161 cgcgaaacgg cgataagcga gagaatgtaa ggagatggcg tgcatcgggc aaactccgta
     2221 cagagaggag aaaattcgcg caatcatggc acctttcgct aaggcgtgca agccaggact
     2281 ttgcgaccag acaagccatc aacaaggcaa aaagttcata ataagaaaat caaacaatac
     2341 agctaacagg aactcttgtg gtcatcggcc cttttatcaa cgctagtgca gtcttactgg
     2401 gtggcgttct cggcgcactg ctcagccaac gcttaccgga acgtatccgc gtctccatga
     2461 catctatttt tggtctggca tcgctgggga ttggtatttt actggttgtg aaatgcgcca
     2521 accttccagc gatggtttta gctacgctac ttggcgctct aattggcgaa atttgtttgc
     2581 tggagaaagg tgtcaataca gcagtcgcca aagcacaaaa tctgtttcgt cactcacgta
     2641 agaagccagc gcatgaatct tttattcaga attatgtcgc gattattgtc ctgttttgcg
     2701 ccagcggcac cgggatcttc ggggcgatga acgaagggat gaccggcgat ccgagtattt
     2761 taatcgccaa gtcatttctt gatttcttta cggcgatgat cttcgcctgc tcgctgggta
     2821 ttgcggtgtc ggtaattagt atcccattac tgatcatcca gctaacgcta gcgtgggctg
     2881 ccgcgctgat attaccgctg accacaccgt cgatgatggc agacttcagc gccgtaggcg
     2941 gtttattgct gctggcaacc ggattacgca tctgtggcat taaaatgttc ccggtggtca
     3001 acatgcttcc agcactcctt ctggcaatgc cgctttccgc cgcctggacc gcctggtttg
     3061 cctgacaatg cgtgcaatag cggcaaagtg atgatagatt gtgcagtctg cagtaaattg
     3121 aagaaatttg attgacgaga cgaggcgaat caggtttaat gcgccccgtt gcccggatag
     3181 ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt ccgagtccgg
     3241 gcaccaaatt catatcaacg gacctccacg gaggtccgtt tttcgtttca gaacgccacg
     3301 atttaagcgt tctgcctaca aatcaattct accgaactca accagattcc ccccacatca
     3361 accccattgt gtgggtataa ttgcgggcat accccagttc gacagaattt gtaccctctt
     3421 taacgcattc agaaggctga actatggcac tgactgacgc aaaaatccgg gctgcaaagc
     3481 ccactgacaa ggcttataaa ctcactgacg gggctggcat gttcctgctg gtacatccta
     3541 atggctcccg ttactggcgt ctccgttatc gtattctggg taaggagaag actctggcac
     3601 ttggtgtgta tccagaagtt tctctctccg aagctcgtac aaaacgggat gaggcccgaa
     3661 aactgatttc ggagggggtt gacccttgcg aacagaaaag agctaaaaaa gtagtccctg
     3721 atttacagct ctcttttgaa catattgcgc gacgctggca tgccagtaat aaacaatggg
     3781 cacaatcaca cagcaataaa gtactcaaaa gccttgagac tcacgttttc ccctttatcg
     3841 gcaaccggga tatcacaaca ctcaataccc cggatctgct tatccctgtt cgtgctgcag
     3901 aagctaaaca aatttatgaa atcgccagtc gtctgcagca aagaatatct gccgtaatgc
     3961 gttatgccgt acagtctggc atcatcagat ataatcctgc tctggatatg gctggcgcat
     4021 tgactacggt aaaacgccag catcgccccg ctcttgatct ttctcgcctg cctgaacttt
     4081 tgtcgcgtat tggcagttac aaagggcaac ctgtcaccca gcttgccgtt atgctgaatt
     4141 tactggtttt tattcgttcc agtgaactca gatacgcccg ctggtctgaa attgatattg
     4201 acaatgccat gtggactatt ccagccgaac gcgaacccct gccaggcgta aaattctcac
     4261 accggggctc caagatgcga acaccacatc ttgtgccact cagcaaacag gctgtagcca
     4321 tactgacaga actacagaca tgggctggtg aaaatggtct gatctttacg ggagcacatg
     4381 acccgcgtaa accaatca
//
LOCUS       DAJTVK010000213         4375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000213 DAJTVK010000000
VERSION     DAJTVK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.216"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..675
                     /locus_tag="OO372_004121"
                     /pseudo
     CDS             1..675
                     /locus_tag="OO372_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            682..1428
                     /locus_tag="OO372_004122"
     CDS             682..1428
                     /locus_tag="OO372_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6343456.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1443..1865
                     /locus_tag="OO372_004123"
     CDS             1443..1865
                     /locus_tag="OO372_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6343457.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1923..2279
                     /locus_tag="OO372_004124"
     CDS             1923..2279
                     /locus_tag="OO372_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343458.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2448..3113
                     /locus_tag="OO372_004125"
     CDS             2448..3113
                     /locus_tag="OO372_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6343459.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            3341..3496
                     /gene="hokD"
                     /locus_tag="OO372_004126"
     CDS             3341..3496
                     /gene="hokD"
                     /locus_tag="OO372_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6343460.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            3746..4033
                     /locus_tag="OO372_004127"
     CDS             3746..4033
                     /locus_tag="OO372_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343461.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            4072..>4375
                     /locus_tag="OO372_004128"
     CDS             4072..>4375
                     /locus_tag="OO372_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6343462.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
BASE COUNT         1181 a          972 c         1200 g         1022 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagatct aaatactgat ctccccctcg ccaaaaacga
      361 gcgtctaaaa aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc
      421 tggcatgagt gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc
      481 gctaacgggg cgatacggga actggaaaaa ttccgtcagc agggttttac acctgagcag
      541 gtgattcgac acagcatcgc caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg
      601 cctgagacgt tgctccgaca ggttaacacc gtctcgttgc cggacagtgc gatcccgcca
      661 ggcttcaggg ggtaacggac catgaaaaat attgcgacag gcggcgttct ggaacgcatc
      721 cgccgactga ccccgccaca tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag
      781 tggcaacttg ctgaaggcca gaaacgtagc gaggagatca accgcctgaa tcgccagttg
      841 cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt
      901 gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc gaaatccatc
      961 gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaacc tggtaccggg
     1021 aagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca gacagtgatt
     1081 gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga cgggcagtca
     1141 ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga tgaaattggc
     1201 attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga tcgccggaca
     1261 gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat gaaaacattg
     1321 ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt gaattttaac
     1381 tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt tcaggaggat
     1441 ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga attgtggaac
     1501 tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc agatgaaaca ggtatcagtc
     1561 gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta tataattctg
     1621 gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac
     1681 tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc tggtcattac
     1741 ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc gagtgccgga
     1801 agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag gaggcgatac
     1861 tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca aaggagagga
     1921 gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     1981 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgccatc
     2041 acgcctctgt acgggttgac gaaaaagagc gtcaggtccg ctgtaaaatc tgcggtgcgg
     2101 ttgtggagcc gtttgactgg atgctctctg tggcaaaaag agaaaccaga ctggcagatg
     2161 atgtaaggct attgcgccag gaggaacagg aaaggcggag aaatatagaa aagctgatac
     2221 agattgagcg taacgcgaaa gcgcggatac gcagggcgac aaaatccaga actgaataat
     2281 taaatttagc actgttaaaa atttaatcct taaccggagg gatttctgca ccctcaaatc
     2341 atcaggagac cacccgaaag ggcggggagc agtcacacat ctgtttccga tagccccgtt
     2401 ctaatgctac actctttgat atttttatga ccccaataaa catatttatg acagttgctg
     2461 atttcaaacg gcccaaattg gagctcccaa acggggcaaa caaactacta ctgcactctt
     2521 gctgtgctcc atgttccggt gaggtgatgg aggcgcttca ggcctcggga atcgactaca
     2581 ccatcttttt ctacaacccg aacattcatc ctcagaaaga gtatttaatt cgtaaggatg
     2641 agaatattcg ctttgctgaa caacacggcg tgccgtttat tgatgctgat tacgacacag
     2701 acaactggtt tgaacgagcc aaaggaatgg aatgggagcc cgaacgaggg atccgttgca
     2761 ccatgtgttt tgacatgcgt tttgagcgga cagcgctgta cgccgctgaa aatggtttca
     2821 gtgtgatcag cagttcactg ggcatttcac gctggaaaaa tatgcagcag gttaacgact
     2881 gtgggcggcg agccgtcgcg cattatccgg gcatggtgta ctgggattat aactggcgca
     2941 agcagggcgg ctcgtcccgt atgattgaaa tcagcaagcg cgaaaaattc tatcagcagg
     3001 aatattgtgg ctgtgtgtat tctctgcgcg ataccaatct acaccgcaaa tctcagggac
     3061 gccctcttat caaaattggt caactccact acggtaaaga agagaaggag tgattttatg
     3121 gggcaccttt ctgattgatt tcatattggc gaggtaagta gaatgactgc gggtgcttga
     3181 ggctatctgc ttcaggcatg aacaccaaaa ggcagataga gaaaagcccc agttaacatt
     3241 acgcgtccgg caagacgctt aacattaatc tgaggccaat ttcatgcttt gcacatgtag
     3301 gttagcctct tacatgccga aaggcaagga gaagcaggct atgaagcagc aaaaggcgat
     3361 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
     3421 agacctctgt gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcgtagacta
     3481 cgaatccagg aagtaagagt gaccgggcgg ggagctgatc ccatccccgc ccacctctga
     3541 tgtgtcaggc atcctcaacg cacccgcact taacccgccc atcgctgtga tctctcagcg
     3601 tttcggcggg ttttttgttg tttatttccg gtgaatttga ttcgcgcacc ttcgcagata
     3661 gaatcgactc acttaagtag cgcgcaggga gaagagggat ggaccccgaa caggggagag
     3721 ctatttatct ggaaggattc tgaagatgaa aatcgaagaa ttgcgtgaaa tttttagtga
     3781 aaatggcctc tatgctgtgc gcgttgagaa tggagcgatt gtcagccatt gccgcattag
     3841 atgtttgcaa tctcaacaaa ggaagagcgg tgctgtgtta ttttattttt gtaatggact
     3901 tctgacggac ggttttattt tgcgtgagga cgaatttgtc acatcattac gggttttgaa
     3961 agagattggt tttaaggctg gttttctgct tttgctgaag aataaactca tctacaatct
     4021 tgagcaggat tgaactcctg ctgtgtaaca ccgtgccacc ggagaaagcc gatggcacat
     4081 atacaactgg tcaaacaaac ctcttccgga ttacttctcc cggcgacgcc ggagagttgc
     4141 gattttctgc atcaaatcaa aataggtgag tggatacacg cagactttaa gcgtgtgtgt
     4201 aactacgcat tccacaagcg ttttttcaaa ctcctgcaac tgggtttcga ttactggact
     4261 ccggtcggtg gggcgatcac gcctcgcgaa cgaaaactgg tgtccggttt cgttgattac
     4321 ctgtgtgaat cagtaggccg ggaacatacg ccagctctga gcgaagccgc agagc
//
LOCUS       DAJTVK010000214         4326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000214 DAJTVK010000000
VERSION     DAJTVK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.217"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..579)
                     /locus_tag="OO372_004129"
     CDS             complement(<1..579)
                     /locus_tag="OO372_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6343463.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYTSYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(642..1091)
                     /locus_tag="OO372_004130"
     CDS             complement(642..1091)
                     /locus_tag="OO372_004130"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6343464.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OO372_004131"
                     /pseudo
     CDS             complement(1088..4180)
                     /gene="ebgA"
                     /locus_tag="OO372_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1027 a         1089 c         1167 g         1043 t
ORIGIN      
        1 gttcgggatc atcgcgtcaa cggtgatggc gtcagcaggc tgtacgccgc caaccagcgc
       61 cgtacctgcc agtaaaatgt aggagagcgt taacagcagc atcagcgttg aagtgacgga
      121 ggtaattggc cccaacattt tcgccccgtt ggtggaaacc caggtggaga aggcgaacag
      181 caccatactg ataatggtgg tggcaaccgg cgtcataata tattcgtatc cgaggaaggc
      241 atacgaagta taagcaataa cgcgcggcaa cagtgaggtg aaaaagaaca ggtttacgaa
      301 ccagtaggta taggcagtaa taaatgccca acgtccgccc agcgaacttt ttacccatgc
      361 gtagacaccg gcttctgagt ttttatttaa cgaaacaaat tctgcgatga tcagacagaa
      421 gggaataaaa taaaaaatcg tcgcgaggaa aaacatcggt gccgaggcca gtccaagatc
      481 aatattatta ttgataacgt tgttaaagct gtaaacggcg gcaaaagtca gcgagagcaa
      541 gccgaatttg ccgattgtat tacgtttggt atcagacata acacatctcc gaaaatgagt
      601 agggtagcgg tggcggcaaa ctccttttgc cgccgtagtt gttatttgtt atggaaataa
      661 ccatcttcga tggtgacttt gagaaccact tttttgaccg cgttattgca gataaaccga
      721 tacgcttcat ggatatcgca aataacgatt tgcccttcgt ggacctcaac ggtttctccg
      781 cagcctttta aatattcacg gtcagtttca tcacgataat attccactac ctgtaatgtc
      841 tctttcggcg catattcaat tttttgctgc ccttgcaggt agtaatgcac ttcaaaatag
      901 cgtcgatgcc cggtaaatag cgcatcggtc gcggagtctg tctccacgcg gtaagtcaat
      961 gagtcaccga tggagtgggc gacgccaggc tgaatgttgt cgatattttc aatcgcttca
     1021 acgcagcgtt gccacttctt gccagaggcg taaatctggc ggaactgttc taagttatcg
     1081 atgatcctca ttgcttattc tcgctgtgca aattcgtgga aaagaaccct gcgccgaact
     1141 catacgacgc caggctttgc gcggtagctt ctccgccaga aaccggcagc aacgtaaagc
     1201 cgtagctgaa gtcacggaac cagacgcgcc aggagtccag cacctcgctg ccccaggagt
     1261 tggagccgag gccaagcagc tggtgatcga ggttcagggt gatgtcatca ctgcgctgca
     1321 gctcgttaca gtgctgggca gcgtggatgt tttcctaggt atagtgccag gcgctgaagt
     1381 taattgggcg ctgcggaacc accagcagac cgttaccgtg gcggttagtc agtgccgtcc
     1441 agcggacatg ctgacggtta ccgttgttct gcgggaaggg atagttctcg aacatggcat
     1501 cgacggtgct gcgccagata tcgatgatgt tagcctgctg gctgtcggcg tagttttcgc
     1561 ccggtccacg accgtaatac gccacctgat cgtattcgcc gttaattccc atggtgaaac
     1621 cgatgcacgg aatgatgtgc ggatagtcgc cgtaacgctc gccggaaagc gccacgttaa
     1681 cctggccatc ggcagcgatg cgccagatgt aggtgcagcg cattccgaaa tcaaacaccg
     1741 gcggggcaat aaccgtgcgg ctgatgatca acacttcacc ctcgctctgt tctacggcaa
     1801 agtcgcgcag atgttcctgc atgatctgca aatgattcgg ctgccacaac ccttcgtact
     1861 cctgcttgtg gttgtcgatc atcggcttga agaagttgat ctttggctcg cgggtcagca
     1921 gcgactcgcc atttacctgc caggatgtcg gtttgccact catttttgag aaggtgatcg
     1981 cgaagttgta gccgcgaacg gtgcagctca aacgatcgtc ttccagcgtc agcggacgcg
     2041 cattatttgg tgcgaaaggc actggctgcg cggtgttttc cttcagcggg aactgataag
     2101 tggcgataga atgcccggct tcgctgtagc gggtgcggga atctttggtc accgtaatgt
     2161 tgaggaacgc ttcgcgggcg tccagctgcg gcagcgtgat ctgcaagggg gcttcgctgt
     2221 tcggcgcaac gtcgcgcagt ttaatctgct gcgtcgcgag cgtttcacct tcggcgcgca
     2281 cctctgcgtg cagggggtag tcatcaagcg tggtaaacca cagtttattt tcgactttca
     2341 actcgccgcg agtcagatcc cgcgcgtgga tttttaccgg cgcgataacc tgtttgtact
     2401 ctttcaggcc cggtcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
     2461 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
     2521 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
     2581 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
     2641 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
     2701 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
     2761 aatgcaccag tcgcgtgtca tccagcgcct tcgctgcgtg gtacatcgcg cggatgttac
     2821 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgcg
     2881 cgtgaatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
     2941 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
     3001 caaacaggcc gtagatatca cacagttcgt aaaaacgcgg atcgttcggg tagtgagcgg
     3061 tacgcacgga gttgatgttg tgctgcttca tcaactggag atctttctcg acgcgatcca
     3121 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
     3181 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
     3241 gttgtggtac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
     3301 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
     3361 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
     3421 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
     3481 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacggtga
     3541 aatcgttaat atgtgttagt tgttttccga tcagataaac atcgcggaag atccccgctg
     3601 accaccacat atcctggtct tccacgtagg tagagtccgc ccattgcatc acgcgcacac
     3661 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
     3721 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
     3781 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
     3841 gataggcacc ggttgggtta tcgctgggaa caaacggcac atcgatgggg aacggaaaac
     3901 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
     3961 tatgccccca gtcagccatt aactcagagg tgaaggcttc aggcacttgc agcgggtggt
     4021 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
     4081 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgt ggcgcaagtc
     4141 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
     4201 aatcggaacc attcatggtg atggtagttt gtgtaattgc tggggagttt agtaaaattt
     4261 ttactaaaaa gtgacaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
     4321 cactgt
//
LOCUS       DAJTVK010000215         4262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000215 DAJTVK010000000
VERSION     DAJTVK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.218"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            288..896
                     /locus_tag="OO372_004132"
     CDS             288..896
                     /locus_tag="OO372_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6343465.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            994..2385
                     /gene="selA"
                     /locus_tag="OO372_004133"
     CDS             994..2385
                     /gene="selA"
                     /locus_tag="OO372_004133"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6343466.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2382..4226
                     /gene="selB"
                     /locus_tag="OO372_004134"
     CDS             2382..4226
                     /gene="selB"
                     /locus_tag="OO372_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6343467.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          964 a         1078 c         1241 g          979 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ctgccggttt tccgctcatc
       61 tgctcatcct tgttttatcg ggttatttgt ctgcttccag gcaaaaccct acagatttat
      121 aagaaagcct taagtgagat atccatcaga tagaaacaat taggaaaata tttatatcac
      181 catgataaat atgtgattta tttggaagta acaaaccatc aacattttct gccctttttc
      241 ccatcaccag gccatactga tatttttcct cgcacaaagg actcggcatg aaactcgtcg
      301 gtagctacac cagcccgttt gtacgcaaac tttctattct gttgttagaa aagggcataa
      361 ctttcgaatt tattaatgaa ctgccctata acgcggacaa cggcgtggcg caatttaacc
      421 cgttaggaaa agtgccggtg ctggtgaccg aagagggcga atgctggttt gattcgccga
      481 tcatcgctga atatattgag ttaatgaatg tcgctccggc gatgttgccg cgcgatccgc
      541 tggagtcgtt gcgggtgcgc aaaattgagg cgctggcgga tggcattatg gatgccgggc
      601 tggtatcggt gcgtgaacag gcgcgtccgg cggcgcagca gtctgaagat gaattgttac
      661 gccagcggga gaaaatcaac cgcagtctgg atgtgctgga aggatatctg gtcgatggca
      721 cactcaaaac cgatacggtc aatctggcga ctatcgccat tgcctgtgct gtcggatatc
      781 tcaatttccg ccgcgttgcg ccaggttggt gtgtcgaccg cccacactta gtcaaactgg
      841 tcgaaaacct gtttagccgc gaaagttttg ctcgcaccga accgccaaag gcttgatgcg
      901 cgatatgtcc tcctgaccca tctcacgtta caatccgtgg ttatgttaaa cgcccttctc
      961 cgtgtgagag ggccttgatc agccaggttt cctatgacaa ccgaaacgcg ttccctctat
     1021 agtcaacttc cggctattga tcgcttattg cgcgatagct ccttcctttc tttgcgtgat
     1081 acttatggtc acacccgcgt ggtggaattg ttgcgtcaga tgctcgacga agcgcgagaa
     1141 gtgattcgtg acagccagac gcttcctgcg tggtgtgaaa actgggcgca agaagtcgat
     1201 gcccggttga cgaaagaggc gcagagcgcg ctgcgtccgg tgatcaacct gacgggaacc
     1261 gtgctgcata ccaaccttgg acgagcttta caggcggaag ccgcggtgga agccgttgcg
     1321 caggctatgc gttcgccagt gaccctcgag tacgatctgg acgacgccgg acgcggacat
     1381 cgcgatcggg cgctggcgca actgctgtgc cgtattacgg gggcggaaga tgcctgtatc
     1441 gtcaataaca atgcggcggc ggtgttattg atgttggcgg ccactgccag cgggaaagag
     1501 gtggtagtat ctcgcggcga attggtggag attggcggcg cgtttcgtat tcccgatgtt
     1561 atgcgtcagg caggctgcac cctacacgaa gtagggacca ccaaccgcac gcacgcgaat
     1621 gattatcgtc aggcggtgaa tgaaaatacc gcactgttga tgaaagtaca taccagtaac
     1681 tacagcattc aggggttcac caaagcgata gatgaagcgg aactggtggc gctcggcaaa
     1741 gagctggatg ttcccgtagt gactgattta ggcagtggct cgctggtcga tcttagccag
     1801 tacggtttgc cgaaagagcc aatgccgcag gagttgattg cggcgggcgt cagtctggtg
     1861 agcttctccg gcgacaagtt gttaggcggg ccgcaggcag ggattatcgt tggtaaaaaa
     1921 gagatgatcg cccgcctgca aagccacccg ctgaagcgtg cattacgcgc ggataaaatg
     1981 accctcgcgg cgctggaagc cacgttgcgt ctttatttac accctgaagc tctgagtgaa
     2041 aaattaccga ccctgcgcct gcttacccgc agcgcagagg tcattcaaat ccaggcacaa
     2101 cgtttacagg ctccccttgc cgcacattac ggcgcggagt ttgcggtaca ggttatgcca
     2161 tgtctttcgc agattggcag tggttcgctg ccggttgatc gcctgccgag cgcggcatta
     2221 acgtttacac cccatgatgg acgcggtagc caccttgagt cattagccgc ccgctggcgt
     2281 gaattgccag tgccgatgat tggtcgtatt tatgacggac gattgtggct ggatttacgc
     2341 tgccttgaag atgagcaacg gtttttggag atgttgttga aatgattatt gcgactgccg
     2401 gacacgttga ccacggcaaa acaaccttat tgcaggtgat tactggcgta aatgctgacc
     2461 gtctgccgga agaaaaaaag cgcggcatga ccatcgatct cggctatgcc tactggccgc
     2521 agccggatgg tcgcgtgcct agttttatcg acgtacccgg tcatgaaaag tttctttcca
     2581 acatgctggc gggcgttggt ggtatcgatc acgcgctgtt ggtggtggcg tgcgatgacg
     2641 gcgtgatggc gcagacccgt gagcatctgg cgattttgca gctgaccggt aacccgatgc
     2701 tgacagtggc gctgaccaaa gccgatcgcg tggacgaagc gcgtgttgat gaggttgaac
     2761 gccaggtaaa ggaggtgctg cgggaatacg gttttgctga ggcaaaactg tttatcaccg
     2821 cagcaaccga aggtcgggga attgatgccc tgcgcgagca tctgcttcag ttaccaggac
     2881 gtgaacacgc cagccaacat agtgtccgcc tcgcgatcga ccgcgcattt accgtaaaag
     2941 gtgccggtct ggtcgtcacc ggtacggcgt taagcgggga agtgaaggta ggcgattcac
     3001 tctggctgac tggtgtaaat aaaccgatgc gtgtacgtgc gctgcatgca caaaaccagc
     3061 caacagaaac cgcccatgcc gggcagcgta tcgcgcttaa catcgcgggt gatgcggaaa
     3121 aagagcagat taaccgtggc gactggctgc ttgccgatgc gccgccagag ccgttcacac
     3181 gggtgattgt cgagcttcaa acccatacgc cgctgaccca gtggcagccg ctgcatattc
     3241 accacgccgc cagccacgtt acgggacgcg tttcgctgct ggaagataac cttgccgagc
     3301 tggtgttcga cacgccgctg tggctggcgg ataacgatcg cttagtgctg cgcgatatct
     3361 cggcccgcaa cacgctggcg ggggcgcgtg tggtgatgct taacccgccg cgtcgcggta
     3421 aacgtaagcc ggaatatctg caatggctgg cgtctcttgc acgggcgcag agcgatgccg
     3481 atgcgttatc tgttcatctg gaacgcggcg cggttaacct tgcggatttc gcctgggcgc
     3541 gccagctcaa cggcgaaggg atgcgcgaat tgctgcaaca gcctggctat attcaggctg
     3601 gttatagctt gttgaatgcg ccggttgccg cccgctggca gcggaaaatt ctcgacacat
     3661 tagcgactta tcatgagcaa catcgcgatg aacctggtcc tgggcgcgaa cgtctgcgac
     3721 gtatggcgtt gccaatggaa gatgaagcgc tggtactgtt gctgattgaa aagatgcgcg
     3781 aaagcggcga catcctcagt catcacggtt ggctgcatct gccggatcac aaagcgggct
     3841 tcagcgaaga gcagcaggcc atctggcaaa aagcagagcc gctgtttggt gacgagccgt
     3901 ggtgggtgcg tgatctggca aaagagacgg gtaccgacga gcaggcaatg cgcctgacgc
     3961 tacgccaggc ggcgcagcaa ggaataatta ccgcgatcgt caaagatcgt tattaccgta
     4021 acgatcggat tgtcgaattt gccaatatga tccgcgatct cgatcaggag tgtggttcaa
     4081 cctgcgcggc ggatttccgc gatcgcttag gcgtaggccg aaagctggca attcagattc
     4141 tggaatattt tgaccgcatt ggctttacgc gtcgtcgtgg aaatgatcat ttattgcgcg
     4201 acgcattatt atttccggaa aaataaggaa atgattaatt aatttttaaa ataaattaat
     4261 ac
//
LOCUS       DAJTVK010000216         4248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000216 DAJTVK010000000
VERSION     DAJTVK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.219"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="OO372_004135"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="OO372_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6343468.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO372_004136"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="OO372_004136"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR6343469.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO372_004137"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="OO372_004137"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCR6343470.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4248
                     /locus_tag="OO372_004138"
     CDS             4086..>4248
                     /locus_tag="OO372_004138"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="activates citrate lyase by acetylation of the gamma
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6343471.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGI"
BASE COUNT         1048 a         1079 c          981 g         1140 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcgt tgacaccaca gtcgaagtat
     4201 ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg tggaattg
//
LOCUS       DAJTVK010000217         4199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000217 DAJTVK010000000
VERSION     DAJTVK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.220"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1361)
                     /locus_tag="OO372_004139"
     CDS             complement(<1..1361)
                     /locus_tag="OO372_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6343472.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="OO372_004140"
     CDS             complement(1426..1545)
                     /locus_tag="OO372_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6343473.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1700..1918)
                     /locus_tag="OO372_004141"
     CDS             complement(1700..1918)
                     /locus_tag="OO372_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6343474.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2272..3750)
                     /locus_tag="OO372_004142"
     CDS             complement(2272..3750)
                     /locus_tag="OO372_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCR6343475.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDVDENNLKVFNVYFDNN
                     EELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSNKNNVDQFIECAKRNE
                     QTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQV
                     KGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNG
                     NCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSSTLNNNDVGFNFKLFPE
                     VKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNY
                     DIQYVNWRTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGV
                     APFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1185 a          919 c          739 g         1356 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cgcctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tctccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcattatt atcaaagtat acgttgaata cttttaagtt
     3481 attttcatca acatcatatt taatacgttt taattgctct ccagatccca taatgacaaa
     3541 ggcgttgcct tcatgatata tacattcaga catcattttt tgtaaagttt caagagcacc
     3601 gcgatacgtc cctgaggcag cttccttaca aattaaattt ataatactat aagctagtag
     3661 ctccatggat tcagaagatt ttctttcatt acaatttcca ctatctataa atgagctagt
     3721 gttaaattcg ttgcttttac taactaacat tgtctattcc tcagttaatg tctacatggc
     3781 tatttttaat tttattattg tttgtcacta taaaaaatcg ctcatttgag acaattgctg
     3841 gcattaacag cttcatatgc tatacatggt acttttaatt aaattagcac aagaatgtta
     3901 aatttaataa acaaaaagtt atttcgctgt atgataaaaa ccacccgtta taatttatta
     3961 gtgaaattcg tttttcgagt gttagaaatt tatatctaaa tagcgttgat taatgagcaa
     4021 agccagactc ctgtaacgct cacaagactc atctacctgc gacgggtatt ctggtcagta
     4081 gtagatgttt aaggcgtggc agagacattt catccttact ctacggcatt ggcccgcata
     4141 caaatcgtgg ttgtgacact cacttatcat cagtgagcga acagagaata gttcagtga
//
LOCUS       DAJTVK010000218         4192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000218 DAJTVK010000000
VERSION     DAJTVK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.221"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO372_004143"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="OO372_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6343476.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="OO372_004144"
     tRNA            complement(330..405)
                     /locus_tag="OO372_004144"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="OO372_004145"
     tRNA            complement(412..486)
                     /locus_tag="OO372_004145"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="OO372_004146"
     tRNA            complement(603..687)
                     /locus_tag="OO372_004146"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="OO372_004147"
     tRNA            complement(696..771)
                     /locus_tag="OO372_004147"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="OO372_004148"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="OO372_004148"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6343477.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO372_004149"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="OO372_004149"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6343478.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO372_004150"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="OO372_004150"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6343479.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1042 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAJTVK010000219         4186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000219 DAJTVK010000000
VERSION     DAJTVK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.222"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..324
                     /locus_tag="OO372_004151"
     CDS             <1..324
                     /locus_tag="OO372_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6343480.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="OO372_004152"
     CDS             551..1816
                     /locus_tag="OO372_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6343481.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="OO372_004153"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="OO372_004153"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6343482.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="OO372_004154"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="OO372_004154"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6343483.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4186
                     /locus_tag="OO372_004155"
     CDS             3627..>4186
                     /locus_tag="OO372_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343484.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1092 a          938 c         1005 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcg
//
LOCUS       DAJTVK010000220         4131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000220 DAJTVK010000000
VERSION     DAJTVK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.223"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..528
                     /locus_tag="OO372_004156"
     CDS             115..528
                     /locus_tag="OO372_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6343485.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OO372_004157"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="OO372_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6343486.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OO372_004158"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="OO372_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="OO372_004159"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="OO372_004159"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6343487.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OO372_004160"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="OO372_004160"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6343488.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OO372_004161"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="OO372_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6343489.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="OO372_004162"
     tRNA            complement(3794..3881)
                     /locus_tag="OO372_004162"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1101 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa a
//
LOCUS       DAJTVK010000221         4079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000221 DAJTVK010000000
VERSION     DAJTVK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.224"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..787
                     /locus_tag="OO372_004163"
     CDS             <1..787
                     /locus_tag="OO372_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6343490.1"
                     /translation="IAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYV
                     NQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQL
                     SDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQ
                     GNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQG
                     SRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            800..1760
                     /locus_tag="OO372_004164"
                     /pseudo
     CDS             800..1760
                     /locus_tag="OO372_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="OO372_004165"
     CDS             complement(1798..2697)
                     /gene="nhaR"
                     /locus_tag="OO372_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6343491.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="OO372_004166"
     CDS             complement(2763..3929)
                     /gene="nhaA"
                     /locus_tag="OO372_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6343492.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1226 a          953 c          861 g         1039 t
ORIGIN      
        1 tattgccaaa acacgtcacc aaattaactt atcgaattcg acctcatttt ccaaagatgg
       61 ctattcctcc aacaatactg gaattactgg catagccggt gaacatgatc agttaaatta
      121 tggtatatat gttaatcagc aacaacaaaa taatgatacc tcgcttggta cgaatttaag
      181 ctggagaact cccatcgcca taatagatgg cagctatagt cattctaaaa acgcctggca
      241 aagtggtgga agtattagta gtggattagt tgtctggccc ggtggtatta atatcactaa
      301 ccagttatcc gatacatttg caattctgga tgcgcctggg ttagaaggtg cacatattaa
      361 tggacaaaaa tacaatcgaa caaacagcaa aggccaggtt gtttacgacc tgataatacc
      421 tcatcgtgaa aaccatctgg tacttgatat agcaaacagt gaaagtgaaa cagaattgca
      481 gggcaatcgt caaattattg caccttaccg tggagcagtt tcttatgtgc agtttacaac
      541 tgaccaacgt aagccctggt atatacaggc actgcgtccc gatggttcgc cattaacctt
      601 tggctatgac gtactggatc tccaggaaaa taatattgga gtcgttggcc agggtagtcg
      661 cctttttatt cgcgtagatg aaattcccac tggaataaaa gttgctctca atgatgaaca
      721 gaacttattc tgtactatta cttttcaaca cgttatcgat gaaaacaaaa catatatatg
      781 ccagtaaaag gagtttacta tgaagtggct tttattaatc accttgagtc tatattcttt
      841 tattgtgcaa agtgctccct gtgcattgac caatgtcggg gaacaacgtg gaacatatat
      901 actcaagcca ctgagtatga aaggtaatct caccgccgaa aaaattgaaa acaccggaaa
      961 tatcattgag tgtgcagact tacaagagac gaccaacgcc attaaagttc attttttaac
     1021 acagaacgct ttaaggttta aagtcggaag tcgtcattat attattaatt tcccctttga
     1081 atctgacggt aaaaaaaata gagcttccag gaaggtcatc ttataattta gaagaaatgc
     1141 tatctgcgat aaatttcacc attccatact ctatcgcatc tgtatcagat agttccaaca
     1201 ccgcagatat ttttccagga actccttttc ctttgttgtt cgaaattgaa gtgagttcat
     1261 gtacagggaa taatactaat tgtaatagcg tccgctttaa ttataattta aatactattt
     1321 tacaggtaaa tttaatgacc tgtggttttg aagatcaatc catcgacact ggacgatttg
     1381 tactgtctcg tatcaataat gaaaattatc agtttcatca tataagattt gactgcatcc
     1441 agggcgagca aggtgaatta gtttttgaac ccagtgatgt tgaatattac tttgaaccag
     1501 tgacgataca ggagtcagga accagcatat taaaaaatga gttagaaaac agtgaggacg
     1561 gtgcgggtca agtaggcttt caaccctcca atgatggtac gaatgaaatc cagtatggta
     1621 aaagcaatta ctactctttt cagcatcccc atgaaggaag taatcagatt cctctattta
     1681 ttcgcccgcg tacatatgga aataatgtct caagcgggca aataatgagc cgagtcaaaa
     1741 tcgtggttat gtacaactaa tctatctccc ccaccttaac tttgggggag gtgccgatta
     1801 acgcgccgct ggactaaaaa gcgcagaata atccgtattg cagattcgct gtaccgccgg
     1861 gtgctgaatc atccgctcag caaaaatagc atggtactct tccatcacat tctcgacgcg
     1921 accaatttct acgacagttt tatcggcata aaagtcatat gcataaagcg ttggggcaac
     1981 gaagattgca ttgtgcatcg caccaaaagc tttcatcaac gcggcatcat caaactcgcc
     2041 gaggatttct acgtttaatc cctgggagtt aaaccagtta agcaatttgc gccctaacat
     2101 tgaacgtcgc ccaggaatca aaagtcgccg ttcttccaga caagccggga acgatttttc
     2161 tggtggtgga tttgtacacc agaaactcac gccacattcg ccaattctca cggagaacag
     2221 gccttcctgc tgcgtagagt ctatcggaca gtcagaaatg atcatatcca gtttatgctg
     2281 acttaattgc tccagcagca tttcgtgggt ggattcgaag cagcgaagat gaatgggctc
     2341 gccttctacc actgcggcgt taagtacgct actgaccagg cgtttggaaa gtgcatcagc
     2401 cacgccaacg tcaaacaata aattggattc tttgcgatag ttcacaatat ccagcatttc
     2461 ctggcttaag gtgaacattt tatcggcata gcgatagacc agttctccca gctcgctggg
     2521 ttcgagacca cgtcccttgc gtttaaataa tttgccttgc aggcgctctt ccagcgctcg
     2581 aatctgtccg gtaatggttt gtggagttaa ataaagcgcc tccgctgcgc caaccacgga
     2641 accttcttta tagacatgcc agaagtaata caagtggttg taattgatat gagacatgct
     2701 catttctctc cctgataaca atgaaaaggg agccgtttat ggctccccgg taaaccgtcc
     2761 tgtcaaactg atggacgcaa acgaacgcgt aaccagctgt atccaattac cgccgaagag
     2821 atagaaccga ccaggatacc gagtttcgcc cagttaatca gttctggatc tacgctacca
     2881 aaggccaggc tggcaataaa gatagacata gtaaaaccga taccgcacag gatccccacc
     2941 gccataattt gctgataagt cgttccctca ggcagatgcg ccagtttcaa acgcagcgcc
     3001 aaccagcaga acagactaat ccccagcggt ttgccaatca gcaagccagc gatgatcccc
     3061 aatggcagaa tggaggtcaa gccatccagc gtgacgcctt gcagtgaaac gccagcatta
     3121 gcaaatgcaa acagcggtaa aatcagatac gccacccacg ggtgcaatac atgctccagt
     3181 cgcttcgctg gagaacgccc atgcttctct ttcaaaggaa taaagaagcc gacaattacc
     3241 cccgccaggg ttgcgtgaac ccccgatttc aacaccgcag tccacaacac cacgccaaca
     3301 agaatataga cgcccgtgcg gcgtacacca cacagattca ataccgcgag taccgcaatt
     3361 gctacagccg cgacgccaag agaggccatc gataagtcat tagtgtagaa caatgcgatg
     3421 ataatgatgg ccccaagatc gtcgataata gccagagcca tcaaaaagat cttcagtgct
     3481 aacggaacac gacttcccaa cagcgccagc acaccaagtg caaaggcaat gtcagtagcc
     3541 gccgggatcg cccacccttc gcgggtaatc ggatcggcat agttaaaggc cagatagagt
     3601 aatgccggaa caatcatccc accaatagcg gcgataaccg gaaatgcggc ctggcgtaag
     3661 ctggctagcg atccttgcat cagttcacgt ttaacttcca gaccgactaa caggaaaaat
     3721 accgccatca gcgcgtcatt tatccataac agcatgtttt tgttgatttc gagtgaacca
     3781 acccggagct gaaccggcgt ctccagaaag tcgtgatacc atccactggt tgcgccgctg
     3841 ttggccatca tcatcgccag gatagcggca atgataagaa taatgcctcc cgaggcatca
     3901 ctgctaaaga atcgatgcag atgtttcact ttttatttct ctttcaggtg aatagatcgt
     3961 ttttactatt ttaccccgca caaatcatcg ttaaaatcag attatagtgt aggaataact
     4021 cgttttttac gagcaattaa aggatttgcg ccatcagcgg tgagatgagt ttttgacgg
//
LOCUS       DAJTVK010000222         4061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000222 DAJTVK010000000
VERSION     DAJTVK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.225"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            36..1182
                     /locus_tag="OO372_004167"
                     /pseudo
     CDS             36..1182
                     /locus_tag="OO372_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1476..2717
                     /gene="agp"
                     /locus_tag="OO372_004168"
     CDS             1476..2717
                     /gene="agp"
                     /locus_tag="OO372_004168"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6343493.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2755..2982)
                     /locus_tag="OO372_004169"
     CDS             complement(2755..2982)
                     /locus_tag="OO372_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6343494.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(3003..3608)
                     /gene="wrbA"
                     /locus_tag="OO372_004170"
     CDS             complement(3003..3608)
                     /gene="wrbA"
                     /locus_tag="OO372_004170"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6343495.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            3660..>4061
                     /locus_tag="OO372_004171"
     CDS             3660..>4061
                     /locus_tag="OO372_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6343496.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVKL"
BASE COUNT         1145 a          865 c          956 g         1095 t
ORIGIN      
        1 gcgtcattat ttattaaatt ttatagacgc tatatatggg tagtaatata catggaatta
       61 gttgcactgc aaataattat ttgaaacagg cctggaacga tataaaaaat gagtacgaaa
      121 aaaatcaaac atattcaatc acgctttttg aaaacacact ggtgtgtttt atgcggttat
      181 acaatgaact cagacgtaaa gtaaatgaag aggatactcc atgtctggaa tgtgaatcac
      241 tagaaaaaga atttgaggaa atgcagaatg ataatgatct atcattattt atgagaacat
      301 tgcgtactaa tgatacacaa atttattcag gggtttcagg aggtattaca tatactatac
      361 aatatgttca aaatgttgat attgttagag tgtccttgcc gggcagaggt tcagagtcta
      421 tcacagattt taaaggttat tattggtatg gctttatgga atatattgaa aacattactg
      481 cgtgtgatga tattttttct gagtattgtt tagatgatga aaatatgagt atccagccag
      541 aacagataaa tacgccggga atatctgatt tagatactgg ttttaaaaga acatgagtat
      601 tcatgggcgc tgggcggcca taagacgcaa aagcaagtat acgaagagtt tattaaaatt
      661 gttgataaac aaacgatcga ggctcttgtt gatacggcat tctataatct cagggaagat
      721 gtaaagacgt tatttggcgt taatctacaa tctgacaaca aaattcaggg gcagggtggt
      781 tttttatcat ggagtttttt gtcttttaaa aaagaattta ttgatagttg cttgaatgac
      841 aaaaaatgca tactgcattt acccgaattt atatttaatg ataacaaggc acgccttgtt
      901 ttagacaccg acccagagca gaaggttaat gaggtgaaaa atttcctcac tgctctttca
      961 gatagcattt gctcattatt tattgtttac agcaatgtgg catcaatctc cttagggaat
     1021 gaatcctttt caacagatga cgaccttgag tatgggtatt taataaacac tggcaatcat
     1081 tatgacgttt acctccctcc tgaacttttt gctcaggctt acgagttaaa caataaggaa
     1141 aggaatgcgc aaattgattt tttaactcgt tatgcaattt aatgacaaag gcatgtgcta
     1201 aaaaccattg ttattagcct cacttctctt attattggta ctattgcctc tgtattagta
     1261 acgccgctga tattctgttt atccacaggt gcaattatca gcggcgtacg cgaggcaggg
     1321 gctaatcagg catagtttgc gtcaaacctt gcctgttttt gaagatgtat atagaaaaac
     1381 aggcgttcaa caagccattt tgcgaacctg ttcccggaaa aaagtcatat ttctgtcaca
     1441 ctctttaatg attgataaca aaagaggtgc caggaatgaa caaaacgcta atcgccgcaa
     1501 ctgtggcagg gatagtttta ctcgcttcaa atgctcaggc acaaaccgta ccggaaggct
     1561 atcagctaca gcaagtgctc atgatgagcc gccataactt acgtgcgccg ctggcgaaca
     1621 atggcagtgt gctggagcag tcgacgccga ataaatggcc agaatgggac gtccccggtg
     1681 ggcaactcac caccaaaggt ggcgtgctcg aagtgtatat gggccattac atgcgtgaat
     1741 ggctggcaga gcaggggatg gtgaaatcgg gggaatgccc gccgccggac accgtttatg
     1801 cctatgccaa tagtctgcaa cgtaccgttg cgaccgcaca gttctttatt accggcgcat
     1861 tcccggggtg tgatattcct gtgcatcacc aggaaaaaat gggcactatg gacccaacct
     1921 ttaacccggt gatcaccgat gattccgccg cattcagtga acaggcggtg gcggcaatgg
     1981 agaaagagct cagcaaactc cagcttaccg acagctacca gctactggaa aaaatcgtta
     2041 actataaaga ttcccctgcc tgtaaagaga aacaacagtg ttcgctggtg gatggcaaaa
     2101 atacctttag cgccaagtat caacaagaac caggtgtttc cgggccgctg aaagtcggca
     2161 actcgctggt agatgcgttt actttgcaat attacgaagg ttttccgatg gatcaggtgg
     2221 cctggggaga aatcaaatct gaccagcagt ggaaggtgtt gtcgaagctt aaaaacggct
     2281 accaggacag cctgtttacc tcaccggaag tggcgcgcaa tgttgcgaaa ccgctggtca
     2341 gttatatcga caaagctctg gtcaccgatc gcaccagcgc accgaaaatt acagtgttgg
     2401 ttgggcacga ctccaacatt gcctctctgt taacggcgct ggatttcaaa ccgtatcagt
     2461 tgcatgacca gaacgaacgc acgccgattg gcggcaaaat cgttttccag cgttggcatg
     2521 acagcaaagc caatcgcgat ttgatgaaaa ttgaatatgt gtatcagagt gcggaacagt
     2581 tacgtaatgc cgatgcgtta accctgcagg cacctgctca gcgtgtgacg ctggaattaa
     2641 gcggttgccc gatagatgcc aacggtttct gcccgatgga taaatttgat agcgtgttga
     2701 atgaagcggt gaaataacag aaaactcccc cgcgagaagc gggggagtcg ctggttaaac
     2761 gtttttacgt tcgatggtct gttcgcccca aaaaagcgaa tctttatcgg ccttagcaaa
     2821 ggctttgact aacacttcat cgctaccttc ttcccaaatc ttttctgcca ttttttcgtc
     2881 gtacccggcg acttcgaaaa tggcctcggc tatttccggc gacgtattgc gcagagatgc
     2941 ccattcaccg acgtgatgag ctttcgcttc ttgagttggc atgcgtatcc tcctgttgaa
     3001 gattagccgt taagtttaac tgccagacct gcgacatatt ccccttgata acgagcaata
     3061 gacagttctt cctggcttgg ctggcgtgag ccgtcaccgc ctgcgatggt ggttgcgccg
     3121 tacggcgtac cgccgcgaac ctgtgaaacg tcaaataatt cctgcgctgc gtagccaatg
     3181 gggacaatca ccatgccgtg atgcgcaagg gtcgtccagg ttgaagtaat agtttgttcc
     3241 tgaccgccgc cagtaccggt ggaactaaag acgctcgcca gttttccgta tagtgcgccg
     3301 gaagcccaca ggccgcccgt ctggtcgagg aaggtacgca tttgaccgga catgttgcca
     3361 aagcgggtag gtgtaccaaa aataatggcg tcgtaatcgg ccagttcttg cggggttgca
     3421 accggtgcag tttgcgtttt accgcctgct ttttcaaata attgcggcgg catggtttcc
     3481 ggtacacgct taacgacaac ttctgcgcca tcgactttgc ttgcaccctc agcgactgcg
     3541 cgtgccatcg tttcaatatg tccgtacatg gaataataaa gcactagtat tcttgcctta
     3601 acactcatat cattgttctc ctgttcaatg aaccacttag ataagcatag tccgaagata
     3661 tgaagctgct agtgacatac atcaggtgga gtactaaaga gcaagactca ggagactcct
     3721 taagacggca aacaaacctc attgatgctt tctactctaa gcacaaaaac gattattacc
     3781 ttcttcctgc tcacagatat gttgataagg gtaaatctgg ttttcaccag caacataaga
     3841 atcaaggttc tgacttcaga cgtatgtttg aaaatgttat gtctggggct attccagaag
     3901 gttccttgat tgttgtagag aactttgacc gcttcagtag ggctgatatc gacacagcta
     3961 tagatgatgt caggcagata ctgaggaagg gggtctctat actgactctg ggtgatggtg
     4021 aactctacga caagtcagcc ttaacagacc ctgtgaagtt g
//
LOCUS       DAJTVK010000223         4053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000223 DAJTVK010000000
VERSION     DAJTVK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.226"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..83
                     /locus_tag="OO372_004172"
     CDS             <1..83
                     /locus_tag="OO372_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6343497.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="OO372_004173"
     CDS             459..566
                     /locus_tag="OO372_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6343498.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(582..2261)
                     /gene="bcsG"
                     /locus_tag="OO372_004174"
     CDS             complement(582..2261)
                     /gene="bcsG"
                     /locus_tag="OO372_004174"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6343499.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLIRAQIAITAA"
     gene            complement(2258..2449)
                     /gene="bcsF"
                     /locus_tag="OO372_004175"
     CDS             complement(2258..2449)
                     /gene="bcsF"
                     /locus_tag="OO372_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6343500.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2446..4017)
                     /gene="bcsE"
                     /locus_tag="OO372_004176"
     CDS             complement(2446..4017)
                     /gene="bcsE"
                     /locus_tag="OO372_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6343501.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1040 a         1007 c         1053 g          953 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcg aacgtacggt ttatcctggt attgaataac tactgcattt
      661 gagttctcgg agaccggtgc tgtttgtggc aacccactgg tgagtttttt ccagtcaaca
      721 ttgtcttcgg tgaaaatctt gccatcaaga acgcgaacca ccagatcgga gatagctagg
      781 aagctgctcg gttggtcaat gacaatcggt gccccctgat gtggtgcctt catgccgaag
      841 aatttcaccc caacggggac gtctgtgata gacgggctag ggatatcacg taggccagat
      901 acctgcattc tgtcgccctt cagcgcgccg ccgtgttccg gcaccacgac caccatcact
      961 ttacgacccg atttctccag ttcagtaaag aaggcgtcca gttcatcaaa gaatttctgc
     1021 gcccgcgctt tgtaatccgc tgttttgctg acccccggat aatggttgcc gtcatgcagt
     1081 ggaagcgtgt tgtagaacgt ggcactacgg ctgtttttat ctttttcggt aacgtccagc
     1141 cagcggttaa gcacggcggt atcgtcataa accggcgaac catcaaagcc cagcaaaata
     1201 accggcagat ttgtttgatc catcaattca gtctgcatgc cgccattttc gcgaacttct
     1261 ttcaaaaaac cgccgaactg gccgttatgt cccatcatca ggtgctgggt aaagcccagt
     1321 ttcgaaaggt tatcaaacag atagcagtcg ttatttgccg gttgatacag attagtgtgc
     1381 gaagtctgcc cgcagctggc gcgcagtaaa cggatcgccg ccgggccact gtaggaggtg
     1441 gcggagttaa agttcttgaa ctcaatatcg aaatgcgacc acagtggatg cgacatcaac
     1501 ccggcggctt ctatatccga ccaggaaagc gaacagatgt taatcaccag tagttcaaat
     1561 ggctgagcat cagcgggcag cgaagacggg aaggtcgatt tacgtttcgc ctccgcgtta
     1621 tagaaattat tcagccaggc gttaaggttc gccgttgttg gcggtgcagt ttgtgcgggc
     1681 atatcaccca ctaccgatgc gccacccgtc gccgcaacgg ttgccgctgc gttaccaccc
     1741 gtcgttgtta cagtggtcgt cggttgtccg gctggccaca aggagaaact tggtcccgcc
     1801 agggtaagta cgttcagcca tagcagtatg gcaaccacaa aaacggtaat gcgaatccat
     1861 tgtgacagga ataaccaggc cactaataaa acaaaaatgg ccccaatcat ctgccagtta
     1921 ataaagcgtg tgacaaggtc gattaaataa tcggtactga accccgccac ctgcgaaccc
     1981 tggctcatta tgctttccgg gccaggcaac caggtgtcat gccagaacaa agcaaagccg
     2041 atcggcaggg caatccagtg gcgcaagcga tgcaggctgt agcgcggaat gggcatcagc
     2101 agaaacgtgg caaacaccaa attgaggagc ggatggaagt taagatatcc cgcccacaac
     2161 aggccgaact taaccagaaa atagaagttc cagccggaaa ggccgcgcca gtattgccag
     2221 agggaagaag gcatggcggt attttgcgta aattgagtca ttttttggtt gccctggctt
     2281 tttccgtgcg gcgtaacgtc ccggccggtt taacataacg aggtttagca aagaataaac
     2341 gtaaggtgtc gcgaatgcgt cgcaaaaagt gccgcgccag atagcccagc gggaaaaata
     2401 tcagtgcgca aacgacaata atttcaatga tatcgctgat ggtcatcatg atgagcgctc
     2461 cacagcatca tctaacagtc gcatgggttc tggtattcgt cgccagtggc gaccatcgtg
     2521 ctcggcattg atgaccggtt tagaactttg cgttaaaggc agcggcatgc cccattgttc
     2581 tggggcaagc aagcgcatct gcaccagctc ggcactgatt tgatcatctt caaaccagac
     2641 catacggttt gagaaaatgt cgccagtagg caatgggaaa atatgattca acgcggtatc
     2701 cagatcgtta atccgacaga atgagagaaa cagcaccagc cgattaccgc caatggtcat
     2761 gatatcgccg gtacggttag ggcgacacag cgtcagggct tgttcaacgc ggatacccgg
     2821 taccggacgt agggcaacca gaacgccttt accgtgggca ggtaatagag ggttattcat
     2881 catgttgttg acggcattac agaacacatc ccacttctgg aaaccacgca gtttgagcgg
     2941 ctgggtcatt gacagcaagg tagtgatatc ttccggcaca tagcgactaa acttctgccc
     3001 ttgcacgctt tcgatcatcg tcagacaacg ggagagtggc gcattccacg gaataaccat
     3061 atttgcaccg caggccaata acaaacgttc atcggtggcg cgcaggctag cggtattttc
     3121 ccgcacgagg attttcatcg cactaccgcg ctggcgacgc agggtatgaa tgctgcgggc
     3181 cagtggctcg atttgcgcat tttgctgtaa agaaaagacc accgtcgccg cctgagcggt
     3241 acgggcttca ttgaacagga cttcgttatt gttgaacagt tgccagtgtt ccgatagcgg
     3301 cggcgcacct tccagtacag caacattact cagaatgcgt ttttcgtcgc tgcgtggttg
     3361 gatctccgcc tcttcgcttt gaactaatgt ccagatacca ttttgttgct gaacgctaag
     3421 ctgctgacgg gcgctgaccc ctttttcgtt gcaccagaag gcaatatcca gcaaatgttg
     3481 gtcaccctga aaacgcaaac tggcaagacc aaaaagtgaa cggtattcct caagcaacaa
     3541 tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac aatggtttaa
     3601 cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag gaatgttttg
     3661 ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa tagaacactg
     3721 caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa ataattttat
     3781 tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc tgtccatgct
     3841 aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg ccagactgat
     3901 agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca tatgtcgcag
     3961 ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt ccctcatgtc
     4021 accattttgt ctgattatca gtttactagc gaa
//
LOCUS       DAJTVK010000224         4045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000224 DAJTVK010000000
VERSION     DAJTVK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.227"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="OO372_004177"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="OO372_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6343502.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="OO372_004178"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="OO372_004178"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6343503.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="OO372_004179"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="OO372_004179"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6343504.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="OO372_004180"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="OO372_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6343505.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1142 c          970 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAJTVK010000225         4020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000225 DAJTVK010000000
VERSION     DAJTVK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.228"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            168..503
                     /locus_tag="OO372_004181"
     CDS             168..503
                     /locus_tag="OO372_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6343506.1"
                     /translation="MLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTED
                     TIDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLT
                     LSYREVNRD"
     gene            496..978
                     /locus_tag="OO372_004182"
     CDS             496..978
                     /locus_tag="OO372_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343507.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="OO372_004183"
     CDS             complement(1012..1092)
                     /locus_tag="OO372_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343508.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /locus_tag="OO372_004184"
                     /pseudo
     CDS             complement(1128..1552)
                     /locus_tag="OO372_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1982..2472
                     /locus_tag="OO372_004185"
                     /pseudo
     CDS             1982..2472
                     /locus_tag="OO372_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2697..3188
                     /locus_tag="OO372_004186"
     CDS             2697..3188
                     /locus_tag="OO372_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6343509.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            3523..3981
                     /locus_tag="OO372_004187"
     CDS             3523..3981
                     /locus_tag="OO372_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6343510.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT         1502 a          649 c          647 g         1222 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat aattcagcaa gcaatattat ctgctatgag
     2221 catatataaa ttttcgataa cagatgattt aatgagtatg gctgtaaatg aactcataaa
     2281 actgaccaaa atagagaata atgtagacct gaataaattc actactatat gcacagacgt
     2341 tctatccccc ctcgtcacca gacataataa agaaaaaaaa caaacgacat tctacccttc
     2401 tcaaaaatcc cctttttaat tttcattgaa aaaatggcaa ttgatactat gaatccaggt
     2461 catacacatt aaggcattta tgtaatgtcg taattaagat aactaataag gtgaatatta
     2521 gtaaaacaac taatgatgtt attagtttgt agtgaacagt acttttacca ataatgaaaa
     2581 atataccata agcaatgcaa gcttaaaact aataatttaa aaaagataaa tgtaaaaata
     2641 ttaatgagtc aatcaataat aatttagcat cacgagacac atcacagagg aatattatga
     2701 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacg
     2761 cattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
     2821 gaatatatgc atatgcattc gactaccatg aaaagggtaa tataacggac gcagaaattt
     2881 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctaaaa gattttgcat
     2941 cagtatgtca gccaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
     3001 attactcccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
     3061 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
     3121 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
     3181 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taactactat
     3241 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
     3301 aatcattatt tttcaatgcg ttagagaaca atctacttta taaaaattga ataaggctat
     3361 ttcagattac atcactgaat tcctaagata aattagtgaa cacaaccata gatatgtcga
     3421 atgtattttc ctgtagcatt aatgacacat aaatatgaat agccataatt tctattgctt
     3481 aagcaggatg caagaaacca attttttcat agaggttaac taatggactt agaaaataaa
     3541 ttctcatatc attttcttga gggattaacg ctcacggaag atggaattct tactcaagga
     3601 aatgagcaag tttatattcc acagaaagag ttaggtgtat taatagtatt acttgaatcc
     3661 gctggtcatg ttgtactgaa agatatgatc atcgaatcag tatggaaaaa tattattgtt
     3721 agtgacgagt ccctgacaag atgtatctat tctttgcgct gcatttttga aaaaattggc
     3781 tatgatcgtt gcatagaaac aatctaccgg aaaggttatc gtttcagcgg gcaggttttc
     3841 aaaactaaaa ttaatgaaga taatacttca gactattcca tagctatatt ccctttcact
     3901 acttcattga atacactgga tccattaata cttaatcagg aattagtgca aatcatttca
     3961 aataaaaaaa tcgatggtta gactggcccc ctgaatctcc agacaaccag tatcacttaa
//
LOCUS       DAJTVK010000226         3961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000226 DAJTVK010000000
VERSION     DAJTVK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.229"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..242)
                     /locus_tag="OO372_004188"
     CDS             complement(<1..242)
                     /locus_tag="OO372_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6343511.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO372_004189"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="OO372_004189"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6343512.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="OO372_004190"
     CDS             2255..3904
                     /locus_tag="OO372_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6343513.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1032 a         1001 c          928 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 g
//
LOCUS       DAJTVK010000227         3950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000227 DAJTVK010000000
VERSION     DAJTVK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.230"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="OO372_004191"
     CDS             complement(<1..953)
                     /gene="ydcO"
                     /locus_tag="OO372_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR6343514.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTMTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="OO372_004192"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="OO372_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR6343515.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="OO372_004193"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="OO372_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR6343516.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="OO372_004194"
     CDS             complement(3668..3898)
                     /locus_tag="OO372_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR6343517.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1008 c         1000 g          919 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcat cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag
//
LOCUS       DAJTVK010000228         3915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000228 DAJTVK010000000
VERSION     DAJTVK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.231"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..301
                     /locus_tag="OO372_004195"
     CDS             <1..301
                     /locus_tag="OO372_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343518.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="OO372_004196"
     CDS             390..2258
                     /locus_tag="OO372_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HCR6343519.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..>3915
                     /locus_tag="OO372_004197"
     CDS             2295..>3915
                     /locus_tag="OO372_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR6343520.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQ
                     KLSQNKINKFNNYLFRWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICP
                     YALMVYGVGDGGAGPGEEHIERLTRIRNIDGLPHVDFSRVDKFFTYADAFRESLPIIS
                     GELYFEAHQGCFTSESATKAHNRNMENKLHDA"
BASE COUNT         1230 a          721 c          766 g         1198 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaaga agtgagcgta ctgacgaaat cataagtact ctcgaatata
     2941 ttgataatac gttaattacc tttagcaatg aagaaataat taaatgtcaa attgcaatcc
     3001 aatccataat caataaaaac aataatcctt cagagttcag gattatggct actggtcatg
     3061 cccatttaga tatagcgtgg atgtggcctt taagagaagg acgcagaaaa gcaataagaa
     3121 catttgccac cgcccttgca aacattgaaa aatatcctga ttacattttt ggcgctagtc
     3181 agtaccaact ttttcactgg ataaaaaaag attatcccta tttcttcgaa aagttaaaaa
     3241 aacaaatcgc cgcaggccgc tttgagttac aaggttgctt ctgggtcgaa tgcgatttaa
     3301 atctggtaag tggtgaatcg ctgataaggc aaatcatgca tgggacacga ttcacactgc
     3361 agaatttttc aaaaaagata aattatgtct ggcaaccgga tgtctttgga ttccccgcaa
     3421 cgcttccaca aattctcaaa aaatcaggca ttaactatat tgcatcgcaa aagctctcac
     3481 aaaacaagat taataaattt aataattacc ttttccgctg gcagggcctg gatggctcag
     3541 aaattctgat gcataacttt cctgaagaca cttatgatag ccgagcacgt gctcgctctt
     3601 tagaatatat tgaacaaaac tataatgaaa aagagatctg tccctatgcg cttatggtgt
     3661 atggtgtcgg tgatggcggc gctggccccg gtgaggaaca tatagaacga ttaacacgta
     3721 tacgcaatat tgatggctta cctcatgttg atttcagccg tgttgataag ttcttcactt
     3781 acgccgacgc ctttcgcgaa agcttgccca ttatttcagg tgaactttat ttcgaggctc
     3841 atcaagggtg ctttacatca gagtcagcaa ctaaagctca taatcgaaat atggaaaata
     3901 aattacatga tgctg
//
LOCUS       DAJTVK010000229         3908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000229 DAJTVK010000000
VERSION     DAJTVK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.232"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            24..590
                     /locus_tag="OO372_004198"
     CDS             24..590
                     /locus_tag="OO372_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6343521.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="OO372_004199"
     CDS             594..1169
                     /locus_tag="OO372_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6343522.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="OO372_004200"
     CDS             complement(1211..2263)
                     /locus_tag="OO372_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6343523.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="OO372_004201"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="OO372_004201"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6343524.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="OO372_004202"
     CDS             3580..3777
                     /locus_tag="OO372_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6343525.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          987 c          951 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAJTVK010000230         3900 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000230 DAJTVK010000000
VERSION     DAJTVK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.233"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..196
                     /locus_tag="OO372_004203"
     CDS             <1..196
                     /locus_tag="OO372_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343526.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="OO372_004204"
     CDS             207..374
                     /locus_tag="OO372_004204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343527.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            482..715
                     /locus_tag="OO372_004205"
     CDS             482..715
                     /locus_tag="OO372_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343528.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="OO372_004206"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="OO372_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR6343529.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1466..1738
                     /locus_tag="OO372_004207"
     CDS             1466..1738
                     /locus_tag="OO372_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343530.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1812..2798
                     /locus_tag="OO372_004208"
     CDS             1812..2798
                     /locus_tag="OO372_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6343531.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLHHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="OO372_004209"
     CDS             2799..3179
                     /locus_tag="OO372_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6343532.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3900
                     /locus_tag="OO372_004210"
     CDS             3176..>3900
                     /locus_tag="OO372_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6343533.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT         1051 a          869 c         1085 g          895 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg caccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaagatc cagtatccgg atggtaagcc
     3601 gccggtatgg gcaaagtgta cgaaaggtgt gtatccgtct tactgggaag aatggaaaaa
     3661 agtcagggag gtggtaaaag ttgcctgtcc ggagtgtggc ggaaagggtg aggtttccac
     3721 cgcctgtaag gattgccgtg ggcgtggtgt cgccattcat cgtgaagagt cggtaaaacg
     3781 tggtatgcct gttatcagag actgccagcg ttgtggtggt cgtggctatg aaagactacc
     3841 atcaacggag gcatttaatg ctatatgcga ggtgacaaac cagataacac gcgcgtcatg
//
LOCUS       DAJTVK010000231         3866 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000231 DAJTVK010000000
VERSION     DAJTVK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.234"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            752..1468
                     /gene="nanC"
                     /locus_tag="OO372_004211"
     CDS             752..1468
                     /gene="nanC"
                     /locus_tag="OO372_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6343534.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1488..2594
                     /gene="nanM"
                     /locus_tag="OO372_004212"
     CDS             1488..2594
                     /gene="nanM"
                     /locus_tag="OO372_004212"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6343535.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2659..3639
                     /locus_tag="OO372_004213"
     CDS             2659..3639
                     /locus_tag="OO372_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6343536.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         1163 a          801 c          877 g         1025 t
ORIGIN      
        1 gacttcagaa gcgaccagtt acaagccaca aatcaggaaa catatttcat ggtgagaatt
       61 atgatcagcc tgatggcggg taaacacttg ttttcaccac gctcactgag ttagcttgca
      121 aagcttccct tgtttagacc ctaaataaat tttttataaa acaaacagtt aaaagaaatc
      181 ctttagtttt tgaagaccac ctcaagtgtt cgtctggctt cacatggcat cttcctctta
      241 gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct cgttttgttg tatggcttgc
      301 gtgatttttg atctggtata acaggtataa aggtgcaccg cgatagtcaa tgagacaggg
      361 catctcgcaa tctatggcaa acatcacttc agttctttct catcgggtga tgaaaacgca
      421 cttcagtctg aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg
      481 taaaatggat tgagtaagtg atatagctta cgaacattca aatcaattaa acatcagaag
      541 agattttata ctcaggtatt taatctgatt ctctgtttat ttaaataatg tggaaagaga
      601 attttcacag gagaccttat acaaaaatat aaaataagct acccgttgcc aaagacacta
      661 taagcctggc aaaaaatatt acacaacata aatgctaatt gtttatgcgg gctttgtatt
      721 gctttctgta tcctacaaat gagtgaaatt tatgaaaaag gctaaaatac tttctggcgt
      781 attattactg tgcttttcgt caccattaat ttctcaggct gcgacactgg acgtacgtgg
      841 tggatatcgt agcggaagcc atgcctatga gactcgactc aaagtcagtg agggatggca
      901 aaatggatgg tgggcaagca tggaaagtaa tacctggaat accattcatg ataataaaaa
      961 ggaaaatgcc gcactcaatg atgttcaggt tgaagttaat tacgcgatta aacttgatga
     1021 tcaatggacg gtgcgcccgg gaatgttaac gcattttagc agcaacggca cacgctacgg
     1081 accctacgta aaactgtcct gggacgcgac aaaagatctt aattttggca ttcgctatcg
     1141 ttacgactgg aaagcttacc gacaacaaga cttatccggt gatatgtctc gtgataacgt
     1201 tcatcgttgg gatggatatg tcacttacca tattaatagt gatttcacct tcgcatggca
     1261 aacgacgcta tacagcaaac agaatgatta tcgctatgca aaccacaaga aatgggcgac
     1321 ggaaaatgcc tttgttctac aataccatat gacgcccgat attacgccat acatagaata
     1381 tgactacctt gaccgccagg gtgtttacaa cggcagagat aatttatcgg aaaacagtta
     1441 tcgcattggt gtgtcattta aactgtagta gacaggagac agtcacaatg aataaaacta
     1501 taatggcgct tgctatcatg atggcttcat ttgccgcaaa cgcgtctgta ttaccggaaa
     1561 ctcctgtgcc atttaaaagt ggtaccggag caattgataa cgacactgtc tacattggtt
     1621 taggtagcgc aggtacagca tggtacaagc tagatacaca ggccaaagat aaaaaatgga
     1681 ctgcgttagc tgcattccct ggtggaccga gagatcaagc aacctctgca tttattgatg
     1741 gcaatctata tgtgtttggc ggcattggca aaaacagcga gggattgact caggtattta
     1801 atgacgtaca caaatacaac cccaaaacca atagctgggt taaattgatg tcgctcgcgc
     1861 cgatgggcat ggcgggtcat gtgacttttg tacacaacgg caaggcttat gttactggcg
     1921 gtgttaacca gaatatcttc aatggctatt ttgaagatct caacgaggct ggaaaagatt
     1981 caaccgctat agataaaatc aacgcccact attttgacaa aaaagcagaa gattatttct
     2041 tcaataagtt tctgttgtct tttgatccct caacacagca atggagttac gctggcgaat
     2101 cgccctggta cggaacggct ggtgcggcgg ttgtgaataa aggtgataaa acctggctta
     2161 ttaatggcga agccaaacca ggattgcgaa cggatgccgt atttgaactt gatttcaccg
     2221 gtaataattt aaaatggaat aagcttgatc ccgtctcatc accagatggc gtcgctggcg
     2281 gttttgcggg gataagcaat gattctctta tatttgccgg aggggccgga ttcaaaggtt
     2341 cacgagaaaa ttaccagaac ggtaagaact atgcgcatga aggcctgaaa aaatcatata
     2401 gcactgatat tcatctttgg cataacggga aatgggataa atcgggtgaa ttatcgcaag
     2461 gtcgggccta cggagtatca ttgccctgga ataatagttt attaattatt ggcggtgaaa
     2521 ctgcaggcgg caaagcggtg acggattcag ttttaatttc tgtgaaggat aataaagtca
     2581 cagtacaaaa ctaacgcttc agggccccgg taaaggggcc ctgctatcaa tttgtcattt
     2641 gtattacgcg aaaattatat gaacgcaata atatcgcccg attattacta tgttcttacc
     2701 gttgctggtc agtctaatgc catggcgtat ggcgaaggac tgccattacc ggacagggaa
     2761 gatgcgcctc attccagaat taaacaatta gcgagatttg cgcatacgca tcccggaggc
     2821 ccgccatgtc actttaacga cattattcca ctgactcact gcccacacga tgttcaggat
     2881 atgcagggtt atcaccatcc tctggcaacg aatcatcaaa cacagtacgg caccgttggc
     2941 caggcactgc atattgcacg gaaattactg cccttcattc ctaataatgc agggattctt
     3001 atcgttccgt gttgccgtgg cggatcggct tttaccgcgg gcagcgaagg gacatattca
     3061 gaacggcacg gagccagcca tgatgcttgt cgttggggaa cggatactcc gctgtaccag
     3121 gatttagtca gcagaacgcg agccgcactg gcaaaaaatc cgcagaacaa attcctcggc
     3181 gtatgctgga tgcaaggcga atttgactta atgaccagtg actacgcgtc acaccctcaa
     3241 cactttaatc atatggttga agcctttcgt agggatctaa aacaatacca ttctcagctt
     3301 aataatatta ctgacgcgcc atggttttgc ggcgatacca cctggtactg gaaagaaaat
     3361 ttcccgcatt cgtatgaagc tatttatggc aattatcaaa ataatgtttt agccaatatt
     3421 attttcgtcg acttccagca acaaggtgaa agaggactga cgaacgcgcc tgatgaagac
     3481 ccggacgatt taagcacggg atattacggt tcagcgtacc ggtcaccgga gaactggacg
     3541 acggcactgc gaagcagtca tttcagcacg gcagcccgtc gggggattat ttctgacaga
     3601 tttgtagaag caattttgca gttttggcgc gaaaggtgag cggggagttt attcatgggg
     3661 gatgtccttg ttcgttctca gagtagacaa caaaaaacaa ataagatcaa ggtaatatct
     3721 cttcacttgc gggctcgaac agcaggcaat tgccaatata ataatctggc acaagggtga
     3781 aaaacgcgca ccgcataaac cgttgttatt gcttacagat tagccggatg ccttaataga
     3841 catccgcgcc ttttcgatta tggctc
//
LOCUS       DAJTVK010000232         3864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000232 DAJTVK010000000
VERSION     DAJTVK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.235"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="OO372_004214"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="OO372_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="OO372_004215"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="OO372_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCR6343537.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="OO372_004216"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="OO372_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCR6343538.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="OO372_004217"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="OO372_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR6343539.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          846 c          959 g         1071 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc tcgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAJTVK010000233         3858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000233 DAJTVK010000000
VERSION     DAJTVK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.236"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="OO372_004218"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="OO372_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6343540.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSVRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="OO372_004219"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="OO372_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6343541.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="OO372_004220"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="OO372_004220"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6343542.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="OO372_004221"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="OO372_004221"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6343543.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          910 a         1049 c          943 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtgt gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAJTVK010000234         3831 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000234 DAJTVK010000000
VERSION     DAJTVK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.237"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..265)
                     /locus_tag="OO372_004222"
     CDS             complement(<1..265)
                     /locus_tag="OO372_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6343544.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            352..759
                     /gene="nudG"
                     /locus_tag="OO372_004223"
     CDS             352..759
                     /gene="nudG"
                     /locus_tag="OO372_004223"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6343545.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(725..997)
                     /locus_tag="OO372_004224"
     CDS             complement(725..997)
                     /locus_tag="OO372_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6343546.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1233..2576
                     /gene="gdhA"
                     /locus_tag="OO372_004225"
     CDS             1233..2576
                     /gene="gdhA"
                     /locus_tag="OO372_004225"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6343547.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2693..3732)
                     /locus_tag="OO372_004226"
                     /pseudo
     CDS             complement(2693..3732)
                     /locus_tag="OO372_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          932 c          917 g          985 t
ORIGIN      
        1 caagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac
       61 cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc
      121 ccagcgccag aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat
      181 ctggcgtaat gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc
      241 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag
      301 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
      361 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
      421 gcccagagcg atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa
      481 agccagcagc aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg
      541 ggtgagtatg ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc
      601 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
      661 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag
      721 gcgtttatgg ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc
      781 gctggcattg cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
      841 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
      901 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg
      961 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
     1021 ctctgcaact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
     1081 gttatgagat tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg
     1141 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
     1201 caatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
     1261 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
     1321 aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat
     1381 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
     1441 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
     1501 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
     1561 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
     1621 gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc
     1681 tgatgaccga gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg
     1741 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
     1801 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
     1861 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
     1921 ttgaagggat gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
     1981 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
     2041 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
     2101 atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
     2161 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
     2221 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc
     2281 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
     2341 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     2401 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     2461 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     2521 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     2581 taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta
     2641 cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     2701 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     2761 agtgctccca aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     2821 ttctgcgata gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat
     2881 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     2941 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg
     3001 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     3061 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     3121 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     3181 ccattaggca caataacttt cgctatcttc gataccccat tgattctcta aagactcgcg
     3241 tcttttactg atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt
     3301 accaggttca ggctttcgcg gccagaactc tagcgtttca gaccattgct tatgatagaa
     3361 tcgataaggt gcgatcaatt gtagcgcctg taacttctcg atactgagcg gctcaatacc
     3421 tttagcctga taataatgca gttgttcttt ttttgcttta aaaccggccc gaacaataag
     3481 ccccatcata attaatagat aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt
     3541 caaaccgttg gatgttatcg ctgcgaacac aaacattaca gcgacaacac atgttaaata
     3601 aaacccccac ttacaaagca gcatggcctt attttcttta atcatccgtt caaaattact
     3661 attaaatatt tcccagccat taaaagaata cttctcgctc ccaggatggt tttgtaataa
     3721 aacttttttc atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc
     3781 aaaaaaaata tccaacgaat tttcttgatc cgctggatat ttcagaatat g
//
LOCUS       DAJTVK010000235         3798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000235 DAJTVK010000000
VERSION     DAJTVK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.239"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="OO372_004227"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="OO372_004227"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6343548.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="OO372_004228"
     CDS             1028..1486
                     /locus_tag="OO372_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6343549.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="OO372_004229"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="OO372_004229"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6343550.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="OO372_004230"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="OO372_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6343551.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="OO372_004231"
     CDS             2692..3369
                     /locus_tag="OO372_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6343552.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="OO372_004232"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="OO372_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6343553.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          953 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 tccacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAJTVK010000236         3734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000236 DAJTVK010000000
VERSION     DAJTVK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.240"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..181)
                     /locus_tag="OO372_004233"
     CDS             complement(<1..181)
                     /locus_tag="OO372_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCR6343554.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="OO372_004234"
     CDS             425..682
                     /locus_tag="OO372_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCR6343555.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO372_004235"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="OO372_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6343556.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO372_004236"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="OO372_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6343557.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO372_004237"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="OO372_004237"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6343558.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3734)
                     /locus_tag="OO372_004238"
     CDS             complement(3307..>3734)
                     /locus_tag="OO372_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCR6343559.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          953 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatt
//
LOCUS       DAJTVK010000237         3685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000237 DAJTVK010000000
VERSION     DAJTVK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.241"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1328
                     /locus_tag="OO372_004239"
     CDS             <1..1328
                     /locus_tag="OO372_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6343560.1"
                     /translation="QQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQGAVHLMLGVA
                     ASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVFFDRAVDDHR
                     HQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQWTLFYQRLAQ
                     DLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFRKHRKAFSYL
                     QHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVKHKDWQQLVH
                     KYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDWNQRDRIAVR
                     QLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKRYSESVEDYQ
                     IRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE"
     gene            1329..2945
                     /locus_tag="OO372_004240"
     CDS             1329..2945
                     /locus_tag="OO372_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6343561.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(3001..3153)
                     /locus_tag="OO372_004241"
                     /pseudo
     CDS             complement(3001..3153)
                     /locus_tag="OO372_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3176..3553)
                     /locus_tag="OO372_004242"
     CDS             complement(3176..3553)
                     /locus_tag="OO372_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343562.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
BASE COUNT         1061 a          712 c          921 g          991 t
ORIGIN      
        1 ttcagcagct tatttatgag cataccttat tccctttata tgctccgttt gtaggcaagg
       61 agcgccgaga cgaagctatt cggttaatgg agtaccaagc gcaaggtgcg gtgcatttaa
      121 tgctaggagt cgctgcttct agagttaaga gcgataaccg ctttagatac tgccctgatt
      181 gcgttgctct tcagctaaat aggtatgggg aagccttttg gcaacgagat tggtatttgc
      241 ccgctttgcc atattgtcca aaacacggtg ctttagtctt ctttgataga gctgtagatg
      301 atcaccgaca tcaattttgg gctttgggtc atactgagct gctttcagac taccccaaag
      361 actccctatc tcaattaaca gcactagctg cttatatagc ccctctgtta gatgctccac
      421 gagcgcaaga gctttcccca agccttgagc agtggacgct gttttatcag cgcttagcgc
      481 aggatctagg gctaaccaaa agcaagcaca ttcgtcatga cttggtggcg gagagagtga
      541 ggcagacttt tagtgatgag gcactagaga aactggattt aaagttggca gagaacaagg
      601 acacgtgttg gctgaaaagt atattccgta agcatagaaa agcctttagt tatttacagc
      661 atagtattgt gtggcaagcc ttattgccaa aactaacggt tatagaagcg ctacagcagg
      721 caagtgctct tactgagcac tctataacga caagacctgt tagccagtct gtgcaaccta
      781 actctgaaga tttatctgtt aagcataaag actggcagca actagtgcat aaataccaag
      841 gaattaaggc ggcaagacag tctttagagg gtggggtgct atacgcttgg ctttaccgac
      901 atgacaggga ttggctagtt cactggaatc aacagcatca acaagagcgt ctggcacccg
      961 cccctagagt tgattggaac caaagagatc gaattgctgt acgacaacta ttaagaatca
     1021 taaagcgtct agatagtagc cttgatcacc caagagcgac atcgagctgg ctgttaaagc
     1081 aaactcctaa cggaacctct cttgcaaaaa atctacagaa actgcctttg gtagcgcttt
     1141 gcttaaagcg ttactcagag agtgtggaag attatcaaat tagacggatt agccaagctt
     1201 ttattaagct taaacaggaa gatgttgagc ttaggcgctg gcgattatta agaagtgcaa
     1261 cgttatctaa agagcggata actgaggaag cacaaagatt cttggaaatg gtttatgggg
     1321 aagagtgagt ggttaggcta gctacattta atgacaatgt gcaggttgta catattggtc
     1381 atttattccg taactcgggt cataaggagt ggcgtatttt tgtttggttt aatccaatgc
     1441 aagaacggaa atggactcga tttactcatt tgcctttatt aagtcgagct aaggtggtta
     1501 acagtacaac aaagcaaata aataaggcgg atcgtgtgat tgagtttgaa gcatcggatc
     1561 ttcaacgagc caaaataatc gattttccta atctctcgtc ctttgcttcc gtacgcaaca
     1621 aggatggagc gcagagttca tttatttacg aagctgaaac accatatagc aagactcgtt
     1681 atcacatccc acagttagag ctagctcggt cattattttt aattaactcc tatttctgtc
     1741 gaagctgttt gagcagtacc gctttacagc aagagttcga cgttcagtat gaggttgagc
     1801 gagatcattt agagataagg atcttaccca gttcatcgtt tcctaaaggg gcgttagagc
     1861 agtcggccgt agtgcagctt ttggtttggt tgttttcgga tcaagatgtt atggattcgt
     1921 atgaaagtat ttttaggcac tatcaacaaa atagagaaat taagaacggc gttgaaagct
     1981 ggtgctttag ctttgaccct ccgcccatgc agggttggaa attacatgta aaaggacgtt
     2041 cttctaacga ggataaggat tatttagttg aggaaatagt aggtttagaa atcaacgcta
     2101 tgcttcctag cacaacagct attagccatg cctcttttca ggaaaaggag gcaggtgatg
     2161 gtagtacgca gcacatagcg gtttcaacag agtcagttgt tgatgatgag catctacagt
     2221 tggacgatga ggaaacagcc aatatagaca cagacacacg agtcatagag gctgagccga
     2281 catggataag ttttagtaga cctagtcgaa ttgaaaaatc tcgcagggca agaaaaagta
     2341 gccaaactat tttagaaaaa gaagaagcaa caacaagtga aaatagtaat ttggttagta
     2401 ctgatgagcc acacttaggt ggtgtcctag cagcggcaga tgtgggtggg aagcaggatg
     2461 caaccaatta caactctatt tttgctaatc gatttgctgc ttttgatgag ctactttcaa
     2521 ttctaaaaac taaatttgca tgtcgggtgc tttttgaaga aaccttggtt ttgccaaaag
     2581 ttgggcgtag ccgattacat ctgtgtaaag atggctcacc aagagtgatt aaagccgttg
     2641 gggtgcaacg taatggcagt gaatttgtat tgctagaggt ggatgcatcg gatggggtga
     2701 aaatgctttc taccaaagtg ttgagtggcg ttgatagcga aacatggcgg aatgattttg
     2761 aaaagatacg gcgtggagtg gtgaagagct cattgaattg gccaaatagt ttgtttgatc
     2821 aattatatgg acaagacggg catagagggg tgaatcatcc aaaggggttg ggggagctgc
     2881 aagtatcgag agaggatatg gaagggtggg ctgagagagt ggttagagag caatttacgc
     2941 attaaaggaa tgactgaaag agcctgtaaa cccttttgtg taagtgcttt tgccggtcag
     3001 ttaaaggtgg ccatttaaac ggtcaccaaa ttcgatcata aaacggttca tggccggctt
     3061 ccagttgcgg atcggcatcg tccatttctt ggtcgccgcc tggatagcca ggtacaccac
     3121 cttcatcgct gattcgtccg tagggaacac cttgagttca tattaatgga attttctaca
     3181 aatagcctcc gtggttttga ggggggatta cagacgatcc atagtagtaa tccaatgagt
     3241 tcttgagcgc ggcgacatgt ttggacgcct tggcaaaaat tagagcctgc ttgaagtgca
     3301 ggcgagcacg tgcttggacg attgatccat tcgcggtcaa aaactcaatc ttggatgaca
     3361 gcgtgtcagg aaatccaata tcgaagtccc gccccgtgtc aaagtagggc atttcatgat
     3421 caaaggacga agcttcccag gccacttccc taatattcca gccgctagaa acaagatttc
     3481 gtacgacttc atcaactttt ttgctttcca acttgtcaca aagtgcatag gcaagagctt
     3541 tggcggcaga cacgtgcgtt tcctcttaaa cataggtcga acggataccc gaggcaaagc
     3601 ggagcattag tcccaaagca tctccggtag atccggaggc tccatactga aggcatggca
     3661 tcttgggcat tgatatttga aactt
//
LOCUS       DAJTVK010000238         3601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000238 DAJTVK010000000
VERSION     DAJTVK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.242"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1120..1455)
                     /locus_tag="OO372_004243"
     CDS             complement(1120..1455)
                     /locus_tag="OO372_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HCR6343563.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            1584..1931
                     /locus_tag="OO372_004244"
     CDS             1584..1931
                     /locus_tag="OO372_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343564.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1951..2541)
                     /locus_tag="OO372_004245"
     CDS             complement(1951..2541)
                     /locus_tag="OO372_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343565.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(2538..2798)
                     /locus_tag="OO372_004246"
     CDS             complement(2538..2798)
                     /locus_tag="OO372_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343566.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            2896..3601
                     /locus_tag="OO372_004247"
                     /pseudo
     CDS             2896..3601
                     /locus_tag="OO372_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS629 family transposase"
     misc_feature    3177..3293
                     /locus_tag="OO372_004247"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          984 a          860 c          817 g          940 t
ORIGIN      
        1 agtcacataa accaccgatt gagtgacagt tactgaaaca actatttcga cacaaaatct
       61 cgtctattca acatcatgca aatttgtcag tgactgaatt tagtgacaga accctctgga
      121 attctgccgt gtaaccactt atatgcagac ctgccccgtt gctctgggtt ttcaggctgc
      181 gatgagtgat gagcgatgat ttccatacag atgatatatc gactggaatt gattgcctgc
      241 aattagattt agttttattg tgtggatcgt tttagttagt ggggatgaat ttccgataaa
      301 ttaaccaatg gaaaggagat gatatgaata aaaagataac tggattatta ataattagtc
      361 tggcgtcaat tttcttaatt gattgcaccc agtgccatta acatctatct acacaaaaat
      421 agaggaaaac aattttgata tatcaaataa ttacaagcag ctccattctt tttattgttt
      481 gggggattac atctataata attagaacaa agaattattt ttctaagaag aaaacagacc
      541 ttaatataag cttttttata gccttcccta tttctataac aatcctttta ttttgccgag
      601 gtatattata aatataacat aatacatcta tatggggtgt acatatcccc acataaaaac
      661 acatactctc attaacacgg gaagaaacta tgttttccaa ttctcactac atgtacataa
      721 tcgttcttgt actatggttt agtattctca agtacttaca gagaaagaat agcatattac
      781 atcctttatt ttcactaatc ctgacaccac taaccatttt aatcattaga cttatgatag
      841 atttaacata caaccaataa attataaaca aaataaaaat agggaacatt atatgcttta
      901 taaaattttc acattaaaca caaagataaa aacaactatc attacaatat gtgcattatc
      961 atataatctt acagcgcaag cttccccgtc atacagacct cgtgatcgag ttaataagac
     1021 tatgatagaa ctacaaacta acggagtaga acggcaactt ttagataatt cttgtggcat
     1081 aagttctggg gtatttaaat aaaatataag acaggctgtc tatcttacag acagcctttt
     1141 tataataaca gaaagaatat atatcatact taacggaaga gataatatga aaacagtacc
     1201 cgtataaaca gcaagcaatc ccatttttcc aggtgtatca gcaaaaaaaa cgctgttcca
     1261 gaaatcaggc cgggtaaatt tcagagctgt atcttcaata taccattttg caaccggata
     1321 caaaaccatt ccgcagagag aaatacacca gaacagtaat ctgtatttaa aatcataatc
     1381 ccatgacata tacagcatat atcctcccgt cacccatccc caccacatat tattaaaata
     1441 atattttctg ttcatctgcc agttttcctt tcttttgcac ttttgtcagt gtactgatgc
     1501 atgacaacat aaccacagca tgataataat aatcaataac aataagctgt gtcacgttta
     1561 catcatggaa cggaggtgct cctgtgactt ttgagcagaa aaaagcccgg gccattgccc
     1621 tgatggacag caaaaagatg tggcggagca attatgcccc tcctcttctg cgtattctct
     1681 ggcggctggg tatccggctt ccgccattac cctttatgcc gttctggcag gtcacggtgc
     1741 tgacgggggg cttatggggc atttcctggg gatgtgcaat gtggtttatc tactgggggc
     1801 cgtcaggaat ggtggcaggt gaggccatta ttataagtat cacggggggt tttttgtccg
     1861 gactgctcat ggcctctttc cactggtggc gcaggaaagt taaccgactg ccaccatggg
     1921 atgatgtgta aaccgtgcac aggcgtgttc ttacggagcg gcactttcat ttatctgtac
     1981 gcgaacctcc attcctgcat cgtgacaggc cggcagccac tgcgccactt ccagcggatc
     2041 gccctcccgg cgtaccaccc agccttcttt attccataac tgcagacagg tgctgccgtc
     2101 gagacgcacc acaaaatccc cacggcaggc ctgatacgtt atgccgttca gctccaccgc
     2161 cagatcagtg gcagaagtgc cgcctgcggg cagacctgct gccgggacca gcggcagtac
     2221 gacgacttct gccgcacgct gttctgcccg gatgcggtcc tgttctgcca gcagcagagg
     2281 ctgtgccacg gcccggccgg cgtccgtcag ctccactgcc agctgcaggt tcggagcgcg
     2341 cagggtacgc agccagccgg catcctccag acgacggcag gaggcgcgca ggtttggccc
     2401 gtagaccggg gcttccccgc tttgctccag cacccgttct atgtcccgcg tcagcacggg
     2461 tcctggccgt ttttcatcaa gggcagccag cacaatcagc actctccgct gcagtggcga
     2521 cggtcggcgg ctctgagtca tttccagtca tcctccggcc agatgagctt tctgaggcgc
     2581 tgatggtcgg cgtcaatgat ctcctgtctc tttatggcat tcattgaggt atagccattt
     2641 atggccagtt catgccagaa gatcagcacg gctccggctt tggctgactc gcagagataa
     2701 gttacgccgt ggctgtccag attgcattcc cggatgcgtt tttccgcaaa ccgggtcagt
     2761 tgttcatagc tgagtgaaaa cgtcatttcc tgatccatcc tgcacgttcc atctgataat
     2821 gttctgacag gtaaaaatca ttgaaccgcc ccgggaatcc tggagactaa acttcctgag
     2881 aaagaggtaa acaggatgac taaaaatact cgtttttccc ccgaagtccg tcaacgggca
     2941 gtccgtatgg ttctggaaag tcagggcgaa tatgactcac aatgggcgac aatttgttcc
     3001 attgctccaa agattggctg tacgccggag actctgcgtg tccgggttcg ccagcatgag
     3061 cgggataccg ggggcggtga tggagggctc accaccgctg aacgtcagcg tctgaaagag
     3121 ctggagcgtg aaaatcgtga actgcgccgc agtaacgata tccttcgcca ggcttccgct
     3181 tattttgcga aggcggagtt tgaccgcctc tggaaaaaat gatgccactg ctggataagc
     3241 tgcgtgagca gtacggggtc ggaccgctat gcagcgaact gcatattgcc ccgtcaacgt
     3301 attaccactg tcagcaacag cgacatcatc cggataaacg cagtgcccgt gcgcagcgcg
     3361 atgactggct gaagaaagag atacagcgcg tatacgatga aaatcacaag gtatacggtg
     3421 tgcgtaaagt ctggcgtcag ttgttacggg aaggtatcag agtggccaga tgcactgtgg
     3481 cacgtctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag gtccgtacga
     3541 ccatcagccg gaaagccgtt gccgcaggcg accgcgtaaa ccgtcagttc gtggcagaac
     3601 g
//
LOCUS       DAJTVK010000239         3560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000239 DAJTVK010000000
VERSION     DAJTVK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.243"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            130..1377
                     /locus_tag="OO372_004248"
     CDS             130..1377
                     /locus_tag="OO372_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCR6343567.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1906..2106)
                     /gene="torI"
                     /locus_tag="OO372_004249"
     CDS             complement(1906..2106)
                     /gene="torI"
                     /locus_tag="OO372_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HCR6343568.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2229..2573)
                     /locus_tag="OO372_004250"
     CDS             complement(2229..2573)
                     /locus_tag="OO372_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343569.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2815..3417
                     /locus_tag="OO372_004251"
     CDS             2815..3417
                     /locus_tag="OO372_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343570.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1143 t
ORIGIN      
        1 tgcagtaacg gcgaaagggg caatgacagc gctgccatgt cgacaagaac tggaatagtg
       61 agaagtaaac ggcgaagtcg ctcttatctc taaataggac gtgaattttt taacgacagg
      121 taggtaatta tggcactgaa tattccattc agaaatgcgt actatcgttt tgcatccagt
      181 tactcatttc tcttttttat ttcctggtcg ctgtggtggt cgttatacgc tatttggctg
      241 aaaggacatc tagggttgac agggacggaa ttaggtacac tttattcggt caaccagttt
      301 accagcattc tatttatgat gttctacggc atcgttcagg ataaactcgg tctgaagaaa
      361 ccgctcatct ggtgtatgag tttcatcctg gtcttgaccg gaccgtttat gatttacgtt
      421 tatgaaccgt tactgcaaag caatttttct gtaggtctaa ttctgggagc gctctttttt
      481 ggcctggggt atctggcggg atgcggtttg cttgacagct tcaccgaaaa aatggcgcga
      541 aattttcatt tcgaatatgg aacagcgcgc gcctggggat cttttggcta tgctattggc
      601 gcgttctttg ccggcatatt ttttagtatc agtccccata tcaacttctg gttggtctcg
      661 ctatttggcg ctgtatttat gatgatcaac atgtgtttta aagataagga tcaccagtgc
      721 gtagcggcgg atgcgggagg ggtaaaaaaa gaggatttta tcgcagtttt caaggatcga
      781 aacttctggg ttttcgtcat atttattgtg gggacgtggt ctttctataa catttttgat
      841 caacaacttt ttcctgtctt ttatgcaggt ttattcgaat cacacgatgt aggaacgcgc
      901 ctgtatggtt atctcaactc attccaggtg gtactcgaag cgctatgcat ggcgattatt
      961 cctttctttg tgaatcgggt agggccaaaa aatgcattac ttatcggtgt tgtgattatg
     1021 gcgttgcgta tcctttcctg cgcgctgttc gttaacccct ggattatttc attagtgaag
     1081 ctgttacatg ccattgaggt tccactttgt gtcatatccg tcttcaaata cagcgtggca
     1141 aattttgata agcgcctgtc gtcgacgatc tttctgattg gttttcaaat tgccagttcg
     1201 cttgggattg tgctgctttc aacgccgact gggatactct ttgaccacgc aggctaccag
     1261 acagttttct tcgcaatttc gggtattgtc tgcctaatgt tgctatttgg cattttcttc
     1321 ctgagtaaaa aacgcgagca aatagttatg gaaacgcctg taccttcagc aatatagacg
     1381 taaacttttc cggttgttgt cgatatctcc ctatccctca accggaaaat aataatacta
     1441 aagtgcttag cccggctaat aatcacctaa tccaaacgcc tcattcatgt tctggtacag
     1501 tcgctcaaat gtacttcaga tgcgcggttc gctgatttcc aggacattgt cgtcattcag
     1561 cgacctgtcc cgtgtatcac ggtcctgcga attcatcaag gaatgcattg cggagtgaag
     1621 tatcgagtca cgccatattt cgctatcagg attctgtgtg atggttacat cgcccggccc
     1681 agggctgttt agtcatcagc gctttctgac agtgctgaga tttcaacctg ttgcagtaaa
     1741 aatgagtaga tataaggcaa gtgtgctgcc aaacccatct tttacggggt gaaggtagat
     1801 ttcgtttgaa gggtatctgg tgtcccctgc agacatctac ttgaggcggc aggggattga
     1861 ttggaatgct gttttttaga tttgaaaaat attttacccg ctattttacc cattggcgcg
     1921 gcttaagagc ttatttttga attcacaatg gtcacgatat aaccatcttg ctcgtccgtg
     1981 gataactttg gctttaggca ggtcgccgga cttaatccgg tcatagatga aggtcttacc
     2041 gaagccagta tcagccatga tgaatttcaa atcaaccagt gaatcaggtt gtagttcgtg
     2101 ttgcatgagt gctatctccg aatatggaat cgaacctgca aatcaggcaa taaaaagccg
     2161 cattgatgca gcaatggtag gtctggatat cttgagaaat gaacaggcct catcgagtgt
     2221 gaggcgggtt agtccttgcg tagctcgctg attcttctgt aagtctctgg tgctttgttc
     2281 ccgtacgtct tcatttcaga cttcaacaga gcaacgagtg aatcccattc gttgaggatt
     2341 cctttgaatg ccggaacgcg ctttgcaacc ttgtcgaatg aatctctgat ttctggaatc
     2401 tgctcaacaa gtgcaacgca tcgccgaaag tctgctgcgt catggagagc gccgaagtga
     2461 tgaccataga tattcttttt caggccacat gcgattgagg caagagttgc gctactgatg
     2521 ccgacatcgc cagttgattg ccatttcaaa accttcatag ccaaatctga catttcttgt
     2581 ctccataaaa caaaactcgc cgtagcgagc tcagataaaa gaaatccccg tcagtgcgag
     2641 gatgctgttc attgctgcta tacacttttt tgctctcaac gtaagcggta gcccattctg
     2701 ttgggttggt cagttgcttt taggaaatgc tctttacccc ttaaacgtcg gctgaaagag
     2761 ctaaaatcca tgcaaaaaat ttacgcaatt ttgtgtatta ttgtgcagta agtaatgagc
     2821 tattttctgt gcaaaaaatg gatgataaat ttgtccgggg caggaaaaat tttatgggcg
     2881 ctaaacatga aaaaagattc gtatccttat ttgatttgca tgacagtttc agggctgatc
     2941 tttattttcc ttttcttctg gtggcgggca gatatctaca gggtcacgtt tcttaatcag
     3001 agtatatccc actattacat tctgtttagc atgggaatag cttttctgtt atctctgttt
     3061 tgggttaaga aggggatagt aaaacaaagc ggctggaaga gtctgtcagc ataccttaag
     3121 gtttatgcag ggatatgcat attttctgga ttttttctga ttatacccct tacaacacta
     3181 acttattttt tgcctggaga gacatcgtct tatgttgcac cgtatcggta tacttccggt
     3241 agttcaaaaa gttgttctgg agctgaggtg gatgaccccg atctacatga gaatattcgc
     3301 atttgctatc cgtatggcaa ttatgagtac gataatatta tctatgttga aaagaaaatt
     3361 aatacattag gtgcggtagt gacatatgca cagaccgcgc gtgatgatac tgaatgagat
     3421 agtatatagc gggcaagttt tagttaattt atcgaggtaa tataatttac ctcgactcgt
     3481 ttattctggt atgaatattt cgctttacga tcgattttta tctgatgata tcatgcggtt
     3541 ttcatatact gacttactgt
//
LOCUS       DAJTVK010000240         3546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000240 DAJTVK010000000
VERSION     DAJTVK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.244"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..143)
                     /locus_tag="OO372_004252"
     CDS             complement(<1..143)
                     /locus_tag="OO372_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343571.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="OO372_004253"
     CDS             complement(189..530)
                     /locus_tag="OO372_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343572.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="OO372_004254"
     CDS             complement(699..890)
                     /locus_tag="OO372_004254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343573.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OO372_004255"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="OO372_004255"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6343574.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="OO372_004256"
     CDS             complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="OO372_004256"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR6343575.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          924 a          983 c          784 g          855 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat
     3481 gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag
     3541 gcttgc
//
LOCUS       DAJTVK010000241         3507 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000241 DAJTVK010000000
VERSION     DAJTVK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.245"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1523)
                     /locus_tag="OO372_004257"
     CDS             complement(<1..1523)
                     /locus_tag="OO372_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6343576.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYL"
     gene            complement(1520..1819)
                     /locus_tag="OO372_004258"
     CDS             complement(1520..1819)
                     /locus_tag="OO372_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6343577.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1826..2146)
                     /locus_tag="OO372_004259"
     CDS             complement(1826..2146)
                     /locus_tag="OO372_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343578.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2139..2336)
                     /locus_tag="OO372_004260"
     CDS             complement(2139..2336)
                     /locus_tag="OO372_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6343579.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2390..2842
                     /locus_tag="OO372_004261"
     CDS             2390..2842
                     /locus_tag="OO372_004261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343580.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2900..3076
                     /locus_tag="OO372_004262"
     CDS             2900..3076
                     /locus_tag="OO372_004262"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343581.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(3188..3397)
                     /locus_tag="OO372_004263"
     CDS             complement(3188..3397)
                     /locus_tag="OO372_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR6343582.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          881 a          939 c          744 g          943 t
ORIGIN      
        1 atgagatagc cgcagaagtc caccagcgaa tcgccctcgc gttttatcac cagagcttct
       61 tctgatttct gctgctcata cagcaggcgt ttagcttcgt cctggtcaga aaaacgagta
      121 agcaggtggc gaatcacaac cgccagctct ccttctattt tttctgccag catggggtcg
      181 cgttcgttct ccggtacaac ttccgaaaaa ttgaatatca cccgacgacg tgagatcccc
      241 ccgctgcggt cactgaatga catggcgtta ttgttaaccg ccagcactac tgccggaata
      301 cgcgttgagt agggggcttt gtgtttcggg tcaattgcca ccttgtcacc gcctgtaatg
      361 gccttaatcc ctgccccatc accagcgtag cgggtcatat ccggcatgat aatcagcgaa
      421 aagccaacca ctaacgcgcg ttcccttgca tcttccagcg ccttcatgct tgccgatact
      481 gtgttggcct tacccgccag catggtgcaa atctccgcca tcacgctttt accacttccc
      541 cctggacctg ttacctcaat gaataactgc cagtcgtacc ggttcgccag caccatgaat
      601 aatacagcca gtacgcgatc cgccttgcgg tcattctcag ccaccgaacg gcgtaaccac
      661 ttccagaaat tcggcgcatg tgttgccagc gtttccccct ctgctggtgg gctgaaaggt
      721 aattcactgg caattaacaa ccagtcgttt ttgttatgct cccgaaaatt acctgttctg
      781 gtatcaaata ccccgttact gaatccaatc aggttacggg ctgtattccc cattacaggc
      841 aaacttaact tcatggtatc gaccgccgat ttaatggcgt tctgcgaata gctgatctcc
      901 gcatcaatga aaatctgtgc catagctcgc tgtaattctt tatcctgtac tggctcccat
      961 acaacgccgt tgtaatggtg aacagtgtca gagtcagcat gaatcgccag ttcaccgcca
     1021 taatgtgcca ggagaacttc gccgcgttga cttgctccca tctggttaag cgccagtgat
     1081 gaagcgttat cgtcttttac ccgctctttt ttctttacag gcagttcaac tacctttttc
     1141 ttttccgcct gctctgcccg ttcacgttcc agatattcgc gccagttctc ccgtttctgg
     1201 ctgtgcattc cttcagggta ataatcagca tccctgacac ctgccgctgc cagtttctgc
     1261 ccgatggtat taacaagccc cggacgcaat aaccccgcct ggtagagacg cacccgatag
     1321 cgtccgtccg gtacgatttg cagcttatcc agttcggcaa gttgttgttc tccaagccag
     1381 acaggaggca cgctgtcgcc agccagtcgc ccgtcctgtt cctgccactg cttcgcatgt
     1441 gcccacgcat cattacccgc aaaaatgatg acttccgtca ttttgtcacg cggctggtgt
     1501 tttaaatttg gcgctttttt catttctgct ctctccacgc ggcaatcatg tttttcagtt
     1561 cctgtagttt tttatcaaca tccatacatg acacatggtt atttctggaa agcgggattt
     1621 cccgcctgaa tctgctaata aagatctcca cgttcagcga actatgaaat gaatagccat
     1681 cacgaataaa atacacacgg tcaaacatca gttcttttac cgttactctg ttaccgttct
     1741 tatccagata aatagcgccg gggataattt tggggtgtgc ataaccgctg gcagtcaagc
     1801 cagataaata cgttctcatg attatttatc tcagatttga atcagtattc gctttctcta
     1861 tggcatttaa tgcatctgtg gcattttcaa tggtgcaccg taacgaaata tcaaactgcc
     1921 caagcattgc cagtaacaag ccaatattac ccatgtcaat gcgcatagcc ttttcgtcat
     1981 attcctcatt ttctgacgca tgccacatca ggctaccaat tgacgcaaca gccattgata
     2041 tattgtcagt agccccatcc gcagcggaat aaaccttttt agcaatatca tgctcacagt
     2101 taaaatgcgg attaatcagg tactggtaat tggtcatgtc aggcatggca cacctcctga
     2161 cgaatacggg cggcgaatac agcaacacaa cctgacgggc aacggttacg cgcttcgcgt
     2221 tccgtccagg cggttacgtg gatgatttga gattctccgg cactcagggc cagaaaacgc
     2281 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
     2341 gctcctcgtt tacgttgttg gttagaagcc ctgcgagtgg taacgacact tgcggggctt
     2401 cgtcgtttca attgctgaat tgcatgtatc agcacttgtg gtattcacat tacatttagg
     2461 tgaataccat ttcaagtctt ttttggtatt cacttttgta ttacactgca tcccgttatt
     2521 aatgggaggt gcaaacatgt ccaggagttc tgttaacaat aagtcacagc aactgaatgc
     2581 cagatttcca catgaagtag tgagtggcat tgaggcatcc ctacagccag gggaaactaa
     2641 agcgaatttt atagttacgg ctgtacgcgg tgagatcgcc aggcgccagg cagaaggaag
     2701 tggagaaaat ccccttgtgt cttcactgga tgccctagct aatgtcgaac aaatcggcat
     2761 caaggcagcg gaggaaatcg ggcagcttat caccgttgca cgcgaagaac tccagcgccg
     2821 caaggccaaa gaatcagaat aatcactatc agcgccgtgg tgtgaggaac tccggcgcat
     2881 tgctttacag gcgcacacaa tgaccaacga agaatcaacc aaaaaaacat cacctgcacg
     2941 aaaaagacga cgcagaaaga aagagcatga atcagaacga ttcgcgccgt gttcttttgc
     3001 ccttgagaag taccttaaag aatactcatc aaaggagaga gcttcgcagt tatggcaacg
     3061 cactgaaaca gactgatagc attgcccacc agcctgatag cggctatcat tgacatgctt
     3121 atgtttagtg tttccaaact ggcgaccgcc ccaggtcgcc tttgttttat gtgtcatatg
     3181 ctccccttta cactgcctta cctgaattaa tgcgatcccg gcttttaacc cattccataa
     3241 cctcggacag cagccaccct acagaacgac cgcccagatt aagacgtgac ggaaagcgcc
     3301 cttttttctc cagttcgtaa cgtgtagtgc ggcacactcc agttaactta cgacattcat
     3361 cctcacggat tacgcgatct tcatttattt cacgcataca aaaacctaca taaaaattac
     3421 gtatataaac ttttctctta gctgtagata tatgagatcg gatattactt agattctttt
     3481 tcacctctta aattaaaaac acaacca
//
LOCUS       DAJTVK010000242         3318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000242 DAJTVK010000000
VERSION     DAJTVK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.248"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO372_004264"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="OO372_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6343583.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO372_004265"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="OO372_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR6343584.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="OO372_004266"
     CDS             complement(1251..2825)
                     /locus_tag="OO372_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6343585.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3318
                     /locus_tag="OO372_004267"
                     /pseudo
     CDS             3080..3318
                     /locus_tag="OO372_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          849 a          790 c          711 g          968 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actcagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtaccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatg
//
LOCUS       DAJTVK010000243         3286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000243 DAJTVK010000000
VERSION     DAJTVK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.249"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..619
                     /locus_tag="OO372_004268"
                     /pseudo
     CDS             1..619
                     /locus_tag="OO372_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            616..1095
                     /locus_tag="OO372_004269"
     CDS             616..1095
                     /locus_tag="OO372_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HCR6343586.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1149..2099)
                     /gene="corA"
                     /locus_tag="OO372_004270"
     CDS             complement(1149..2099)
                     /gene="corA"
                     /locus_tag="OO372_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6343587.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2469..2807
                     /locus_tag="OO372_004271"
     CDS             2469..2807
                     /locus_tag="OO372_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343588.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKV"
     gene            complement(2818..>3286)
                     /locus_tag="OO372_004272"
     CDS             complement(2818..>3286)
                     /locus_tag="OO372_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6343589.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT          942 a          767 c          692 g          885 t
ORIGIN      
        1 gttccggaac aaaaatatca caacgtgaat gcactgatat tttatttgca gaaatgaaag
       61 aactagcaaa aatgttttcc ttttttggac aatataaaac acttatcgaa gatctgattg
      121 aacattttag atatggaaat ggcagcaatt ttcatagcca acaattaaat ttatcttttc
      181 atgaaaaaat aaatatggtt ataatagtcc aatacgaata atcaaagaat gtatcgaaaa
      241 tggcattaat tcaactccct cgacaggata ccaacctctt atcctgcaaa gcattaaaac
      301 caaattactt tcatcaagac taaataaatt taatgacttc gaggacagtt ttaatggctt
      361 aggaatcagt gttcatgata tttcagctca aaaaatatct cttttaagtt ttcaaaaata
      421 cgctatagga tggagtgcaa caattcattt tgtagcacag gaccacttcg gtcttgatgt
      481 aactgatatc aaaaacaaga cttacagcaa atatagattt tttcgcatat ggttcttttt
      541 acaacggcat aaagactttg ctttcaaacc ttttttcacc aattttaaca ctatcgaaag
      601 aatcgaaaat tatctatgaa aagcaaactc cagaaaatta tattatgttt atttcttcta
      661 tgctgcatct ataatttatg gacattgcgt cctgtacaga ttttgtatac atactctgat
      721 gctggcaatt cagtctttct ggtcgttgat catctgccat ggactgacag cgataaaata
      781 aattggtatc tgaagcatca gaatgaaatt aaaaatcaac atcccctacc agaaggtagt
      841 tggcatacct ggtatgtcat tgatatcggt aatggcttca ctgactataa aaaatatatc
      901 gaagggccgt atgaagatct ctactgtttc ccgacaatta agagcaatga taattgcatc
      961 gtgaaaaatt acttaatggt tattaatgaa tacccttatc gtaacactca tattggtatt
     1021 aacgatttta ctgagtatca attaacacaa gaaaataaaa tagagcgagt tttcaatccg
     1081 catgacttta aataagacaa tttttagaaa taagccggat aaaaaaatag ctttatccgg
     1141 cactcatatt acagccagtt cttgcgctta aagtacagat acggtgccag gcccgcgagg
     1201 atcataaaga taatcgcgcc tgggtagccg aaactccact tcagttctgg cataaactca
     1261 aagttcatcc catagctgga agcaacgagc gtcggcggca ggaataccac ggataccacc
     1321 gagaagattt tgatgatgcg gttctgctcg atgttgataa aacccatcgc cgcctgcatc
     1381 aggaagttca ccttctggaa cagggattcg ttatgcggca gcagggattc gatatctcgc
     1441 aggatttcac gcgcctgctc cagttgccca cccggtaaac gcgctttacg caccaggaag
     1501 ttgagcgcgc gctgggtatc catcagacac aggcgaactt tccagccgat atcttccagt
     1561 tccgccagag tggagagcgc ctcgtcgtac tcatcgccct gatgcccttc cataatcacc
     1621 cggctcaact gctccaggtc gctatagata ttttcaattt catctgccaa ctgttcgatt
     1681 ttggtttcga acagatccag cagcagctcg taggcgttac cgtctaccat cgactggcta
     1741 cgggcacgca tacgatacag acgaaaagcg ggcagttcac gctcacgcag agtaaacaga
     1801 cgaccatcac ggatggtaaa tgccacagtg gagttaccgg cgtgatcttc cgcatcttca
     1861 aagaagaaga aggagtgaat atgcaggccg tcgtcgtctt caaagaaacg tgccgatgct
     1921 tcgatgtctt ccagttccgg gcgggttgcc aggctctggc caagttcaga ttgtacgcgc
     1981 agtcgctcgt cgtcgtccgg ttcgacaaga tcaatccata ctgcatttac aaggggttgt
     2041 gactcttcga cttccagccg ggtcagtcgg ttattttcca gttgaaatgc gctcagcatg
     2101 accgggactc ccaatgctta aaatatcgga cagttcggtg ggcacacaga aacaaattgg
     2161 gtttcagacc attaaacagc ctgactcagc gcgacgggaa aaattgaggt cgctgacaac
     2221 cgctaaggct atcagcaaaa agggatagcc ttaggagttg atcctggatg acaggatagt
     2281 gagccagtat ctactgggtg tgtccaaggc gaatgtcctc ttagagtgat cgtgcgcgca
     2341 tgttacgcca gcaaaatttt gccgtcaaca cgcaacagaa caccacagaa caatagttga
     2401 acttcgcgct ttttggtact cgtcaaggtc gctaacagga taaaaaaatc caacatccag
     2461 gacctataat ggcgcatcgg ctactcattg gtaaaggaat gatcaccttg aatctcaaac
     2521 ggatttttct cgccctcacc ttacttccct tatttgctgt tgccgctgat gattgcgcac
     2581 tctcagatcc gatgctgacc gtacaggcgt ataccgttaa tcctcaaaca gagcgggtga
     2641 aaatgtactg gcaaaaagcc aatggcgaag cgtggggaac tttacatgct ctgctggtgg
     2701 atatgaatag tcagggtcag gtgcagatgg cgatgaacgg cggcatctat gatgaaagct
     2761 atgcgccgct cggtttgtac atcgaaaacg gtcagcagaa ggtgtaatgc cagtcagtta
     2821 agcaactgac tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac
     2881 tctcgggaag gccctttccc ttctcgtcgg taatttcaca agttgtccca tacttgcaag
     2941 atcgcgcatc agctccggta tacgtcccgg tgaagcgccc tgcaatgtca tcagcattct
     3001 catcaccatt ccgcatgatt ctgagaaact cagttgattc ggccagtaac ctttcagatg
     3061 ttccgccatt ttaatcatct gatatctcac cagattataa gccagtaaga caccccacag
     3121 ctcttgctcc acaagctccg gctttttact tctcagcgtc agcctgctca gttgcatcgt
     3181 ctgttttatc tccctgtatc ccagttcgat ttcccagcga tgactgtaca gatccgccat
     3241 ttctcctccg gggaagcgca tggcgtccgt catcgacgtc agcaga
//
LOCUS       DAJTVK010000244         3285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000244 DAJTVK010000000
VERSION     DAJTVK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.250"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..229)
                     /locus_tag="OO372_004273"
     CDS             complement(<1..229)
                     /locus_tag="OO372_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343590.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="OO372_004274"
     CDS             403..1116
                     /locus_tag="OO372_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6343591.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="OO372_004275"
     CDS             1148..1906
                     /locus_tag="OO372_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343592.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="OO372_004276"
     CDS             1952..>3285
                     /locus_tag="OO372_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6343593.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          790 c          854 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAJTVK010000245         3219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000245 DAJTVK010000000
VERSION     DAJTVK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.251"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            7..510
                     /locus_tag="OO372_004277"
     CDS             7..510
                     /locus_tag="OO372_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6343594.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(556..972)
                     /gene="rraB"
                     /locus_tag="OO372_004278"
     CDS             complement(556..972)
                     /gene="rraB"
                     /locus_tag="OO372_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6343595.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1134..2138
                     /gene="argF"
                     /locus_tag="OO372_004279"
     CDS             1134..2138
                     /gene="argF"
                     /locus_tag="OO372_004279"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6343596.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2210..>3219)
                     /locus_tag="OO372_004280"
     CDS             complement(2210..>3219)
                     /locus_tag="OO372_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6343597.1"
                     /translation="LLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDD
                     LNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPYI
                     NDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLFF
                     NLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSIL
                     VNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGY
                     VVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGWN
                     N"
BASE COUNT          893 a          719 c          675 g          932 t
ORIGIN      
        1 taaactatga ataacattgc gccgcaatca cctgtaatgc gtcgcctgac gctgctggat
       61 aatcctgcta tcgcccgcgt cattcgtcag gtatccgccg aatacggtct taccgctgat
      121 aaaggctaca ccgtcgccga tccgaatctt gacgagctgt atcaagtgta tagtcagcct
      181 ggccatgcat attgggtggt tgagtacgag ggtgaagtgg tcggcggcgg tgggattgcg
      241 ccattaaccg ggagtgagtc ggacatttgc gaactgcaaa agatgtattt tcttccggct
      301 attcgcggta aagggctagc gaaaaaactg gccttaatgg cgatggagca ggcacgagag
      361 atgggcttca aacgctgcta tctggaaacg accgcttttt taaaggaagc cattgcgctt
      421 tatgagcatt tgggctttga gcatatcgac tatgcgcttg gctgcacggg ccatgtcgat
      481 tgtgaagtgc ggatgctacg cgaactctaa aaaaatgccc gctagtatgt gctatcgggc
      541 attttaatca attaattagt ggcgaacccc gtcatcgtct tcatcgacaa aatcttcatc
      601 gtcgccatct tcgccgttag gatcttcaaa gtaagtgccc caaccgtcgt actcaacgtc
      661 aaatttctcc gccagcgtca tcagttgttc aacctgggca tcgatcagat cggcattcaa
      721 cgcgcactcg ctgaggatgt cgcagcaaat cacgatatca ccatcttcaa cttccagctc
      781 ttctggatcg gtcacttcgt aaccgagttt aaacgcttca actgccgctt tttccagagt
      841 ttccagatcg tctgcggaaa gatggtgttc gatggtgtac aacgcgtccg gatcgctgcc
      901 atcttccagt aattcttcaa taatcaaacg tgtttcttca cgctgttctt ccagttgttc
      961 cgggtttgcc atggctcatt cctcttaatg tcctgccgat actcttattg tcacacacca
     1021 ctgacattgc ctccaccttt gtgataaaga tttatgcttt agacttgcaa atgaataatc
     1081 atccatataa attgaatttt aattcattaa ggcgttagcc acaggaggga cctatgtccg
     1141 ggttttatca taagcatttc ctgaaattac tcgatttcac gccagctgaa ctcaacagcc
     1201 tgctgcagtt agccgcgaag ctgaaagccg ataagaaaag cggtaaagaa gaagccaaac
     1261 tcactggtaa aaacatcgcg ctcatcttcg aaaaagactc gacccgtacc cgatgctctt
     1321 tcgaagttgc cgcatatgac cagggggctc gtgttactta tctcggccca agcggcagcc
     1381 aaattggtca taaagagtcg attaaagaca ctgcccgcgt gcttggtcgc atgtatgacg
     1441 gtattcagta tcgcggctat ggtcaggaga ttgtcgaaac actggcggaa tacgctggcg
     1501 tgccggtatg gaacggcctg accaatgagt ttcaccccac gcagctgctg gcggatctcc
     1561 tcaccatgca ggagcatttg cccggcaaaa cgttcaacga aatgacgcta gtctatgcag
     1621 gtgacgcacg taacaacatg ggcaattcga tgctcgaagc tgcagcgctt accggtctgg
     1681 atttgcgtct ggtcgcgcca caggcatgct ggccggaagc tgcgctggtt acggaatgcc
     1741 gcgccctggc acaacaaaat ggcgggaata ttacgctgac ggaagatgta gctaaaggag
     1801 ttgaaggtgc tgactttatc tataccgatg tatgggtgtc gatgggtgaa gcaaaagaga
     1861 aatgggctga acgcattgca ttgctgcgtg attatcaagt gaacagcaag atgatgcagt
     1921 tgaccggtaa cccggaggtc aaattcctcc actgcctgcc cgcgtttcat gacgaccaaa
     1981 cgacgcttgg caaaaaaatg gcggaagagt ttggcctaca tggcggaatg gaagtgactg
     2041 atgaggtctt cgaatctgcc gccagcattg tatttgatca ggcggaaaac cgcatgcata
     2101 ccatcaaagc ggtgatggtc gcgacgctca gtaaataatt gatatcgcta taaagatgcc
     2161 gggaaataca aaaacatccc ggcgtcacat tattttatgc agtgatagtt caattattcc
     2221 accccaattt ttttattgct atacgtaaat cttccggagg atttaatttt cctaactgtt
     2281 cttcattaat atatccatat atattgattt tgccattccg gaggattttc atcattcttg
     2341 ttctaatttt taattcatta cccatctttt cttcaaaaat cttaacgaca taacccggaa
     2401 gtttatttac aatttctttt gttttttctt catcaccata aacatatcct aactcattaa
     2461 gatacagctg ttcgccctca taacacttaa gtaattgata gaagtaccac ttagcaaaat
     2521 caggttgctt catgaaaaaa tccttcaaaa aaatatcatt tactaaaatg ctgacaggca
     2581 cttcattaaa tagtaactta acatcatcaa ataaccttag tattaagcgt gatttttctt
     2641 gattgcttaa gctaaaatca ttgacatatg caaccaactt atcccaaatc aattcctgta
     2701 atttacggct atttttatag ggttctttat cgagattgaa aaataaggtc tcagctattt
     2761 taattttaga ttgatcgcag ctaaaatctt tagattgatc gcagctaaaa tcaatatgat
     2821 ttatcacgga gcatgcatac tttatctttt cagaatcacc aaaatattgc attcttttat
     2881 taaaacacca atccagtatt cttacatcat taatatatgg ggattttaaa aaagtctcca
     2941 gaatcgaatc gatgaccggt gtaaggttaa cttttgcatc atctaaggaa tcaataagaa
     3001 tatgagacag gttaactttg atgtcattaa tttcattgtc agtagctttc ttattattaa
     3061 gaagataatt atttactgcg ttaaggtcat ctatttcttt cagtatccct ttttcaccaa
     3121 gcaagtatga aatcgattca ttacatacat aaggcattaa tagcagatgt tctttcctta
     3181 ttgctaataa tgcattgttc aatggttttt ctaataaat
//
LOCUS       DAJTVK010000246         3206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000246 DAJTVK010000000
VERSION     DAJTVK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.252"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="OO372_004281"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="OO372_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6343598.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="OO372_004282"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="OO372_004282"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6343599.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="OO372_004283"
     CDS             1223..1492
                     /locus_tag="OO372_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6343600.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="OO372_004284"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="OO372_004284"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6343601.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..3198
                     /gene="dsbA"
                     /locus_tag="OO372_004285"
     CDS             2572..3198
                     /gene="dsbA"
                     /locus_tag="OO372_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6343602.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          820 a          773 c          778 g          835 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgaca ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtttttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatggccgc
      661 cagccagacg cataaatgcc attacccggc gttctcctgc ttgcagatat tctagtaata
      721 aaggctcaat agcacggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagccg ggaagctaaa acatggggaa
      841 tgtaaggcgt atcgcacgga caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tggcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgttg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttca tcattcgtaa tgttttccgg
     1561 atgatgggat gaacaacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcaaggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag agcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gacggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcaca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttggcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgtcc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tcactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt caaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggcgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac agtgaaatat
     3181 ctgtccgaga aaaaataatt caatgt
//
LOCUS       DAJTVK010000247         3175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000247 DAJTVK010000000
VERSION     DAJTVK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.253"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            533..2386
                     /locus_tag="OO372_004286"
     CDS             533..2386
                     /locus_tag="OO372_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6343603.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2537..2752
                     /locus_tag="OO372_004287"
     CDS             2537..2752
                     /locus_tag="OO372_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6343604.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2757..3071
                     /locus_tag="OO372_004288"
     CDS             2757..3071
                     /locus_tag="OO372_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR6343605.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          762 c          885 g          750 t
ORIGIN      
        1 cttccaggcc catccgctcc ggtgggcctt tttactgcag aaaacaggtt ccccgttaaa
       61 tgctatgttg ctcacaattc agtaagttga cagttgcctg tcagactggg catttgttaa
      121 aaaaatttcg catggtgaat ccccctgagc ggaggggcga ctggtgacgg tataatctct
      181 gattatcaaa acgagaatga cgcgggttta gtggcaccgg gctgaactca ccgggaggca
      241 cccggcacca tgtgcatgat gatacagata cgcggcttta gcccctctcc ggaggggttt
      301 tcttgtgggc aaaaaaagcc cgcgctggga gacgcgggcg gcaaggaata aacaataaaa
      361 cgtgaagtaa tatttcagct ggcgaataat accccatagt aatcactctg cgcaactgcg
      421 cggtcttttt cgaattgcgg gctgtcgtct ctcttctgcc attgtcctgt aacttccgga
      481 cttcagcccg ctccttattt tactcacaat attatcccgg ccgggaggat tcatggcatt
      541 taaacactat gacgtggtca gggcggcatc gccgtcagac cttgcgaaac gactgacaca
      601 aaaactgaag gagggctggc agccgtttgg tagtccggtg gccataaccc cttataccct
      661 gatgcaggcg attgcagcag aaggtgatgt ggtcgtcagt ggtgcaactg agccggagtg
      721 gtactacgtc atcgtactgg ccgggcaatc caatgccatg gcttacggtg aaggtcttcc
      781 gcttccggat tcatacgatg cgccccatcc gcgcattaag caactggccc gtcgtaacac
      841 agtgactccc ggtggtgaag tatgcgtatt taacgacatc attcctgctg accattgtct
      901 gcatgatgtt caggatatga gtacgattaa ccatccccgg gctgacctga gcaaagggca
      961 gtacggctgt gtcggacagg gcttacatat tgccaaaaaa ctgcttccgt atatccctaa
     1021 taatgcgggg atcctgctgg taccatgctg tcgtggtggt tcggcattca cccagggcac
     1081 ggaggggaca ttcagcgagt ccacgggggc cagtcaggat tcggctcgct ggggagtggg
     1141 taagccgtta tatcaggatc tgcttttccg cacgaaggca gcattgcaga aaaacccgaa
     1201 aaacgttttg ctggcgatat gctggatgca gggggaattc gatatgacga atgccagtta
     1261 cgcccagcag ccagcagcat ttcttgcaat ggtacagcag ttccgtgctg accttgccgg
     1321 gctggcggcg cagtgtcacg gtggaagtcc ggcatcagtc ccctggattt gtggcgacac
     1381 gacatacgcg tggaaacaag aacacggtac gcaatatgaa gtggtatatg gtgcatataa
     1441 aggtaaagaa tcccagcaga tttattttgt tccctttatg accgatggta gcggagttaa
     1501 tacaccgaca aacaacccgt cagaagatcc tgatattgcc gggtctggtt attacggttc
     1561 ggcatcccga acgaacaaaa actgggtatc atcaaatcgc ccgacgcatt tcagctcatg
     1621 ggcgcgtcgt ggcattattc ccgatcgtat ggcaactgct attctgaacg tagccggtcg
     1681 caccttagcc ttcattagtg gtaaggcacc ggaaatcaaa ccctcgcccg gcggcgacac
     1741 gccatcgggg ccgtctgaag atgcatccat acgcacaatc tccctgttgc cgacagccgg
     1801 agacgctgct gcgcagggct ggagcattaa gaatggcgga gttcagttgt cagatggtgt
     1861 atttaagatc accaagcaga gcaataaagc ctggtccctg acgcgcccgg tggatgacgc
     1921 agtctccctg ctgacacggg gtggcagact gagctgtaag tttcgactgt caggcgcact
     1981 gaccaacaac cagttcggtc tgggaattta tctgtatacc gatgtagcgt tacctgacgt
     2041 cgtggcgatg accgggactg gtaacccgtt cctgatgtcg ttcttcaccc agaccacaga
     2101 cggcaaactg aatctgatgc atcacaagaa agcaggaaac acaaagttgg gcgagttcgg
     2161 gaattacagt aacgactggc tgacgctgga gctggtgttc accgccggca gtgccacggt
     2221 tactccgaaa ctgaatggag tggctggccc ggcattccag gtcataaaag acagtctgac
     2281 actggggctg aatgcgctga cgctgacgga tattaccaaa aatgcagcgt atggcgttga
     2341 gatagaaagt ctggtgctgg agataaatgc accggcatca tcataaaaag tgagccagcc
     2401 aaatggaagg tatcgttaaa ctcaccggta gtgtcagtgg gtcgtctgag atgcctgcat
     2461 gagttatcag agccatcagt acttaactgg tggctttttt tattgttgtc agcttccgga
     2521 taacgggaga cggggtatgt accagatgga aaaaatcaca acaggtgtgt catacaccac
     2581 gtcagcggtg ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca
     2641 gtgggcggca ataggcgtgc tggggagtct gctgtttggg ctgctgacat atctgactaa
     2701 cctgtatttc aaaatcagag aggaccgtcg taaggctgca cggggagagt aattcaatga
     2761 ctcaaaacta tgaactgatt gtgaaaggga tccgcaattt tgagaataaa gttacggtaa
     2821 ctttagcgtt acgggacaaa aaacgctttg acggtgaaat ttttgacctg gacatctcgc
     2881 tggaccgtgt tgaaggtgcc gcgctggagt tttatgaggc agcagccaga aggagcatca
     2941 gacaggtctt cctggatgtt gctgccgggt tatgtgaagg ggacgagctg ttgccagaaa
     3001 cgcgcccctg ttcagaggcg cggtatacca taaaaattaa cagttctgat aactcgatta
     3061 cgggttgtta gctttttgca gttggctttc cagtatcttt cattggtagc atcctgataa
     3121 atatccatga gcgcaaaaat caaatacggc ctgtcagctg ctgttctggc gctga
//
LOCUS       DAJTVK010000248         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000248 DAJTVK010000000
VERSION     DAJTVK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.254"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1258)
                     /locus_tag="OO372_004289"
     CDS             complement(<1..1258)
                     /locus_tag="OO372_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR6343606.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="OO372_004290"
     CDS             complement(1415..1624)
                     /locus_tag="OO372_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343607.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="OO372_004291"
     CDS             complement(1647..2357)
                     /locus_tag="OO372_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6343608.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="OO372_004292"
     CDS             complement(2873..>3112)
                     /locus_tag="OO372_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343609.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          913 a          654 c          507 g         1038 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcca
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAJTVK010000249         3048 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000249 DAJTVK010000000
VERSION     DAJTVK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.255"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..173
                     /locus_tag="OO372_004293"
     CDS             <1..173
                     /locus_tag="OO372_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343610.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="OO372_004294"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="OO372_004294"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR6343611.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="OO372_004295"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="OO372_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3048
                     /locus_tag="OO372_004296"
     CDS             2191..>3048
                     /locus_tag="OO372_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCR6343612.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGF"
BASE COUNT          696 a          711 c          836 g          805 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcaccg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggcgaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggattt
//
LOCUS       DAJTVK010000250         3010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000250 DAJTVK010000000
VERSION     DAJTVK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.256"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            63..1226
                     /locus_tag="OO372_004297"
     CDS             63..1226
                     /locus_tag="OO372_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6343613.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1686..2123)
                     /locus_tag="OO372_004298"
     CDS             complement(1686..2123)
                     /locus_tag="OO372_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343614.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKLRVNSTIMAIDDSIKCIDE"
     gene            complement(2212..>3010)
                     /locus_tag="OO372_004299"
     CDS             complement(2212..>3010)
                     /locus_tag="OO372_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6343615.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          794 a          649 c          674 g          893 t
ORIGIN      
        1 tcaatgatcg gaagtatgcc aaatcgtgat cgattgtgat gccaaactgt gatcggagat
       61 ggatgccaaa tcatgatcga ttcgaatgcc aaactgcgat cgattcagat gccattttgc
      121 agctggcgtg ggcggtcttt aaaatctttc gtattccggt gaatcagtgg acgctggcga
      181 cggcggcgct gggaggcgtg tttctggtga gtggtttgat tttgttgatg aactacaacc
      241 acccttacac ttttaccgcg caaaaggcag tgatagcgat ccctatcacg ccacaggtga
      301 cgggaattgt tactgaagtc actgacaaga ataatcagct tattcaaaag ggcgaggtgc
      361 tttttaagct cgacccggtt cgttaccagg cgcgagttga cagacttcag gctgacctga
      421 tgacggcgac gcataatata aagacgctgc gtgcgcagct cactgaagcg caggccaaca
      481 ccacccaggt ttcagcggag cgcgaccgtc tgtttaaaaa ttatcaacgt tacctgaaag
      541 gcagccaggc ggcggtgaat ccgttctcgg aacgtgacat cgacgatgcg cggcaaaatt
      601 tcctcgcgca ggatgcgctg gtgaaaggct cggtggcgga gcaggcgcag atccagagcc
      661 agctcgacag tatggttaac ggcgagcaat cgcagattgt gagcttaaga gcgcaactta
      721 ctgaagcaaa atataacctt gagcagactg tcattcgcgc gccgagcaat ggctacgtta
      781 ctcaggtact gatccgccca ggtacatacg cagctgcctt gccgctgcgt ccggtgatgg
      841 tcttcatccc cgagcaaaaa cggcaaattg tcgcccaatt tcggcaaaac tcgctgttac
      901 gtctgaaacc tggcgatgat gcggaagtgg tgtttaacgc gctacctggg caggtgttcc
      961 acggcaaatt gactagtatt ttacctgtcg tgccaggcgg ttcttatcag gcgcaggggg
     1021 tattgcaatc attaacggtc gttcccggca cggacggcgt gctgggaacc attgaactgg
     1081 accctaacga tgatatcgat gccttacccg acggcattta cgcccaggta gcagtttact
     1141 ccgaccattt cagccatgtt tcggtgatgc ggaaggtgct gctaagaatg accagttgga
     1201 tgcattattt gtatttggat cattgagggg gaagggggag attatggaac tttgctccct
     1261 gcaagggcta attatttttg catattactt cattggctgc gatgattttt ctttaatatt
     1321 cattatgtta ataagttctg gatgagtgtg ctctcattgg attttttatc cgtcttgttc
     1381 tgccgcagga tgagactata catcgtcgag atcgcaatga tgatatgagt tgttcctcct
     1441 gcgacggctc taatcaaaaa agagcaggag tgcttttcca tctattgcga tgatggcttt
     1501 attatctgaa ggatgacagt cacatttaaa aaatatttaa tctatagatc acaatgtagg
     1561 gtcatggaat atatgtatac aactattttc ttcttcactt ttaattatcg tattcattat
     1621 aaaactaagt taaaaaaact cctagagagt gagttattaa tatattttta aataaaatta
     1681 agttatcatt catcgatgca ttttatagaa tcatcaatgg ccataatagt actattaact
     1741 ctcaatttta tatttttatc atcactacaa atattaactg aatttatatc aatgattttt
     1801 ttattcttat cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga
     1861 tttagatttt tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag
     1921 gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat
     1981 tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag
     2041 gcaaaatagc ttagtgaaca cgcttttcgt ccctctttag tactggctga tattagtgcc
     2101 cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca
     2161 ctcgcagaaa tagagaaaaa aatacaatta ttaataactt tgccatattt tttagtccgt
     2221 aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt gcatgtcatt
     2281 agatgaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg gaccgaaagc
     2341 gtttaaacaa ctttgtgtta aaaatccatc acgattatat gtttctgtat ttgctgacgg
     2401 tactaaacgt ataccattag gttttctatt ggttgctcca ggaccattaa cattccagct
     2461 ccatacccct tgaggtattg gacctctatt ttgtaagtgt gtacaaccag gattattttt
     2521 acattgcata ttattaccat tattaccaga ggcgacagga atagtaagca catcataaga
     2581 gttatcatca ttttgcgtat ctgaaacgca tgaaagcata cttaaagtta tgctgtataa
     2641 acaggtcgct aatcccaacg ggtcaatatc agttatcgga ttcaacggat actgataaaa
     2701 attccatccc cccttcagtc caatcggatc ctgagtgata tatcgcccct gcagcgggtc
     2761 ataatagcgg tggcggttgt aatacaggcc ggactcctca tcatactgct gccccggcag
     2821 gcggataagc tgctgcagct gatgcgggtt ctcttcattc agcaggttgc cccactcatc
     2881 gtattctgcg caccattctg ttgccccttc cgtgctgata agcaccagcg gcaggccgcg
     2941 atggtcgcag tggtacaggt ggatttttcg cgccggcgtg tacaccgggt ccatctggtt
     3001 ttgcatctgc
//
LOCUS       DAJTVK010000251         2995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000251 DAJTVK010000000
VERSION     DAJTVK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.257"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            19..1077
                     /locus_tag="OO372_004300"
     CDS             19..1077
                     /locus_tag="OO372_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCR6343616.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1163..2779
                     /gene="bglH"
                     /locus_tag="OO372_004301"
     CDS             1163..2779
                     /gene="bglH"
                     /locus_tag="OO372_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR6343617.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2806..>2995
                     /locus_tag="OO372_004302"
     CDS             2806..>2995
                     /locus_tag="OO372_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HCR6343618.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSL"
BASE COUNT          839 a          632 c          746 g          778 t
ORIGIN      
        1 gatcggctgt ttgatgaaat ggcgcaggcg gggatcaggc cgctggtaac gttatcccat
       61 tacgaaatgc catatgggct ggtgaaaaac tacggcggtt gggctaatcg ggcggtcatc
      121 gatcacttcg agcattacgc ccgcacggtc tttacccgct accaacataa agtagcgtta
      181 tggctgacgt ttaatgaaat caatatgtcg ttacacgcgc cattcacggg cgtggggctg
      241 gcagaagaga gtggcgaggc ggaagtttat caggcgatcc atcatcaact ggttgccagt
      301 gcgcgggcag ttaaagcctg tcatagcctg atccccgaag cgaaaatcgg caatatgctt
      361 ctcggtgggc tggtttaccc cctcacctgc cagccacagg atatgttgca ggccatggaa
      421 gagaaccggc gctggatgtt ctttggtgat gttcaggcgc gtggccagta tcccggctat
      481 atgcagcgtt tcttccgcga ccacaatatc accattgaga tgactgaaag tgacgcagaa
      541 gatttaaaac atactgtaga tttcatctct tttagttatt acatgactgg ttgtgtttcc
      601 cacgacgaaa gcattaataa aaatgcgcag ggcaacatac tgaatatgat ccccaatccg
      661 catctgaaaa gttcagagtg ggggtggcaa attgatccgg ttggattacg ggttctgtta
      721 aatacgcttt gggatcgtta tcaaaaaccg ttatttattg tcgagaacgg attaggcgca
      781 aaagacagcg ttgaagcgga tggttcgata caggacgatt atcgaattgc ctatttaaac
      841 gatcacctgg tacaggtaaa tgaagcgatt gccgatggtg tggatattat ggagtacacc
      901 agttgggggc caattgattt agtcagtgca tctcattcac aaatgtctaa gcgctacggc
      961 tttatttatg tggatcgtga tgataatggc gaaggaagcc tcacaagaac gcgtaagaaa
     1021 agcttcggat ggtatgcaga agtgatcaaa acgcgggggc tgtcattaaa aaaataacca
     1081 ataaagcacc ttaattatcg tcgcattcag aacagtctgg atgcgatacg ttaattcttt
     1141 ctttgcacca taaagggata ttatgtttag acgaaatctt attacctctg ccatcttatt
     1201 aatggcaccg ttagcctttt ctgcacaatc attggctgaa tcattaacgg tggaacaacg
     1261 ccttgagtta ttagaaaaag cgttaagaga aacgcaaagc gaactcaaaa agtataaaga
     1321 tgaagagaag aaaaaatata cgccagcgac ggtgaatcgt agcgtaagta cgaatgatca
     1381 agggtatgcc gccaatccgt tcccgaccag tagtgccgca aaacctgatg ctgtactggt
     1441 caaaaatgaa gagaaaaatg ccagtgagac aggttcgatt tattcttcca tgactctgaa
     1501 agatttcagt aagtttgtga aagatgaaat tggctttagt tacaacggct attaccgttc
     1561 tggttggggg accgcctctc atggttcacc taaatcatgg gcgattggtt ctctgggccg
     1621 ctttggtaac gaatactccg gctggtttga tttgcagtta aaacaacgtg tctacaacga
     1681 aaacggcaaa cgggttgatg ccgttgtgat gatggatggt aacgttggtc agcagtactc
     1741 taccggctgg tttggcgata acgccggtgg cgagaacttt atgcagttct ccgatatgta
     1801 cgttaccacc aaaggtttcc tgccctttgc gccagaggct gatttctggg tgggtaaaca
     1861 cggtgcgccg aaaattgaaa tccagatgct ggactggaaa acgcagcgta ctgatgccgc
     1921 agcgggtgta ggtctggaaa actggaaagt cggtccgggt aaaattgata tcgcgctggt
     1981 tcgcgaagat gtcgatgatt acgatcgcag cctgcaaaac aaacagcaga ttaataccaa
     2041 tactattgat ttacgctata aagatatccc gttatgggat aaagcgacct taatggtgag
     2101 tggtcgttat gtcacggcaa acgaaagcgc atcggaaaaa gataatcagg ataataacgg
     2161 gtattatgac tggaaagata cctggatgtt cggcacatct ttaacgcaga aatttgataa
     2221 aggtggcttc aacgaattct ccttcctggt tgcgaataac tctatcgcca gtaactttgg
     2281 ccgttatgct ggcgcaagtc catttaccac ctttaatggt cgttattatg gtgatcacac
     2341 cggcggaaca gcagttcgtc tgacctcgca gggcgaagcc tatatcggcg atcattttat
     2401 tgtggctaac gcgattgttt actccttcgg taacgatatt tatagctacg aaacaggcgc
     2461 acactctgat ttcgaatcta ttcgtgcggt tgttcgcccg gcctatattt gggaccaata
     2521 taaccagaca ggtgttgaac tgggctattt cacccagcaa aacaaagatg cgaatagtaa
     2581 taaatttaat gagtctggtt ataaaaccac gctcttccat acctttaaag tcaataccag
     2641 tatgttgacc tcgcgtccgg aaattcgttt ctacgccacg tatatcaaag ctctggaaaa
     2701 cgaactggat ggcttcacct ttgaagacaa taaagacgac cagtttgctg tcggtgcgca
     2761 ggctgaaatc tggtggtaat ggaacttaat gaaaggaata ttacgatgaa tataaaaatt
     2821 gctgctttaa cgctggctat tgccagcggt atttctgctc agtgggccat agcagcggat
     2881 atgccagcca gcccggcacc cactattccg gttaaacagt atgtgactca ggtcaatgcc
     2941 gataacagcg tgacctttcg ctactttgcc cctggggcaa aaaacgtctc cttag
//
LOCUS       DAJTVK010000252         2966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000252 DAJTVK010000000
VERSION     DAJTVK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.258"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..216
                     /locus_tag="OO372_004303"
     CDS             <1..216
                     /locus_tag="OO372_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR6343619.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="OO372_004304"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="OO372_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="OO372_004305"
     CDS             complement(415..597)
                     /locus_tag="OO372_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6343620.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="OO372_004306"
                     /pseudo
     CDS             696..1586
                     /locus_tag="OO372_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="OO372_004307"
     CDS             1589..2122
                     /locus_tag="OO372_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6343621.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="OO372_004308"
     CDS             complement(2151..2678)
                     /locus_tag="OO372_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6343622.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2966)
                     /locus_tag="OO372_004309"
     CDS             complement(2680..>2966)
                     /locus_tag="OO372_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6343623.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          838 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaa
//
LOCUS       DAJTVK010000253         2939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000253 DAJTVK010000000
VERSION     DAJTVK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.259"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            37..264
                     /locus_tag="OO372_004310"
     CDS             37..264
                     /locus_tag="OO372_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6343624.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="OO372_004311"
     CDS             complement(265..639)
                     /locus_tag="OO372_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HCR6343625.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="OO372_004312"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="OO372_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="OO372_004313"
     CDS             complement(2099..2674)
                     /locus_tag="OO372_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6343626.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          696 c          688 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt agatttgttg tctatagcta
     2761 tagtgactct tctatgtaga cattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAJTVK010000254         2893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000254 DAJTVK010000000
VERSION     DAJTVK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.260"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..493
                     /locus_tag="OO372_004314"
     CDS             <1..493
                     /locus_tag="OO372_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR6343627.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="OO372_004315"
     CDS             493..1083
                     /locus_tag="OO372_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR6343628.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..2110
                     /locus_tag="OO372_004316"
     CDS             1070..2110
                     /locus_tag="OO372_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6343629.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2113..2658
                     /locus_tag="OO372_004317"
                     /pseudo
     CDS             2113..2658
                     /locus_tag="OO372_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2682..>2893
                     /locus_tag="OO372_004318"
     CDS             2682..>2893
                     /locus_tag="OO372_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6343630.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          752 a          633 c          824 g          684 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattaccccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctg
//
LOCUS       DAJTVK010000255         2877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000255 DAJTVK010000000
VERSION     DAJTVK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.261"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="OO372_004319"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="OO372_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6343631.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="OO372_004320"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="OO372_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6343632.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="OO372_004321"
     CDS             1649..2098
                     /locus_tag="OO372_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6343633.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /locus_tag="OO372_004322"
     CDS             complement(2099..2332)
                     /locus_tag="OO372_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6343634.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="OO372_004323"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="OO372_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6343635.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2877
                     /gene="yncL"
                     /locus_tag="OO372_004324"
     CDS             2786..>2877
                     /gene="yncL"
                     /locus_tag="OO372_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6343636.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          756 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttat
//
LOCUS       DAJTVK010000256         2814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000256 DAJTVK010000000
VERSION     DAJTVK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.262"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..989
                     /locus_tag="OO372_004325"
                     /pseudo
     CDS             1..989
                     /locus_tag="OO372_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1005..1754
                     /locus_tag="OO372_004326"
     CDS             1005..1754
                     /locus_tag="OO372_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6343637.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1756..>2814
                     /locus_tag="OO372_004327"
     CDS             1756..>2814
                     /locus_tag="OO372_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343638.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          885 a          566 c          585 g          778 t
ORIGIN      
        1 aggttaaata catacgtaag tttggctcgt aatacttact gggacgccag cagtaatgtg
       61 aattattcat tatcacttag ccgcgatttt gatatcgggc cattaaaaaa cgtctccgct
      121 tctctaacat ttagccgaat aaactgggaa gaagacaacc aggatcaact gtacctaaat
      181 atttcgattc cctggggaac cagtagaaca ttgagctatg gtatgcaacg aaatcaggat
      241 aacaagattt cgcatactgc ttcgtggtat gactcttccg atcgaaataa ttcctggagc
      301 gtttctgctt caggcgacaa tgatgaattc aaagatatga aagcgtcact acgcgccagt
      361 tatcagcata ataccgagaa cggtcgtctc tatctctccg ggacatcaca gcgagacagt
      421 tattattctc tgaatgccag ttggaatggt tcattcacgg cgactcgtca cggagccgct
      481 ttccatgact atagtggtag tgctgactcg cgttttatga tcgacgcaga tggtgcagaa
      541 gatattccgt tgaacaataa acgcgcggta actaatcggt atggcatcgg agttattcca
      601 tcagtcagca gttacataac aacatcatta agcgttgata cccgaaatct tccagaaaat
      661 gtggatatcg aaaactcggt tatcaccacc accttaaccg aaggtgctat tggctacgcc
      721 aaacttgata ccccaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
      781 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
      841 ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
      901 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
      961 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     1021 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     1081 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     1141 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga
     1201 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     1261 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     1321 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg
     1381 tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat
     1441 tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga aatgatggtt
     1501 cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg
     1561 gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata
     1621 cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat
     1681 taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca
     1741 aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat aggttttagt
     1801 gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa taccgtagtc
     1861 attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga taagatctgg
     1921 caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc cccatcacct
     1981 ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg cggtcaggac
     2041 ttttataatc agaactccta tatttttggc gtaacataca atggagttga ttatgattcg
     2101 actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc gggtaccttt
     2161 aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca attatcagta
     2221 acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa tagagttaat
     2281 aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat gagtggcgca
     2341 ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa taatattcat
     2401 gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt tgactttggt
     2461 aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag tgtagtcatg
     2521 acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact
     2581 acggatattt tgagtgatta tagccatctg gatatgggta atggactgct attgaagata
     2641 tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca
     2701 tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc gaacccggca
     2761 gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac ctat
//
LOCUS       DAJTVK010000257         2740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000257 DAJTVK010000000
VERSION     DAJTVK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.263"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OO372_004328"
     CDS             complement(<1..1008)
                     /gene="shiA"
                     /locus_tag="OO372_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCR6343639.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1323..2375
                     /locus_tag="OO372_004329"
     CDS             1323..2375
                     /locus_tag="OO372_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCR6343640.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          593 c          616 g          682 t
ORIGIN      
        1 cgccataaag aaaggaaatg cgctcaacgt tccgattaac gcacctgtga tataaaccct
       61 acggcgacca aaacgatcgg caagccaggc aaaacaggga attgtcaggc agcttaatcc
      121 acctaccagc aaaccaatat taaggaaaag ttcgcgcggt agccccatat tctgggttga
      181 ataattaagt gcaaaggcag taacgatgta catcgtcagc aattcgcaca gtcgtagcgc
      241 aataatcttc aggaaagcac cgggatgtcg taacagcgct tcgataaccg ggatgcgttt
      301 tttcgcggca gcttgataat gttgctgttg ttcaaattcc gcggactcct ccatgccatt
      361 gcgcacccac aatgctccca gtaccagtac gatgctaaac aggaaaggaa tgcgccagcc
      421 ccagcttaaa aactgttcgt cagtcgtcat catactgatc aatgaaacca gtccggttga
      481 aagcagtaaa cctacaccgt agccaacttg tacaccgcta ctgtaaaagg cttttttatt
      541 tttcggtgca ctttcaacgg aaagcaacgc cgcgcctccc cattcgccgc cgacggcaaa
      601 tccctgaatg acacgcagtg tcaccagtaa aataggtgcc caccacccaa tggtcgagaa
      661 tgaaggaaga ataccaatca aggctgtcgc gatgcccatc atccagacgg tcagcattaa
      721 catgcgctta cgtcccagtc ggtcgccaaa gtgaccgaaa atgacaccgc cgagcggacg
      781 gaaaaggaat ccgacgccaa aggtagcaaa tgcggcgagc gttcccatcg ccgggcttac
      841 ttgcgggaaa aactcgcgat taaacaccag tgcggcggtg atgccataga gtaaaaaatc
      901 ataccagtcg acgacggcac cagcgaagct gcctaacgca gctcgtcggg cgcgacttaa
      961 cgaaagcgtc ccttcatcgg gacgagtgga gatgagcgtg gagtccataa tgatcctgtt
     1021 tgtactgtct ttttataatt agtatggaaa aggtaactca cattgccgtc gatctataaa
     1081 cgaaccttat gagactacct cgacagagcg catctgcaaa tgattttatg gctgattagc
     1141 ggcaacatga aaaattcgat ggaagatgaa agcgcaggag ttgctggtgt tgtttaagga
     1201 ttaagcagag gacccacagg ttagagatat gaaacataaa agtccgcata tgcacaaaat
     1261 ggcaccctga tgagtctgat agactgcatt gcattataac cagtagggag gcgaggttag
     1321 gtatgttttt agcatcattg ttgagacgta ttgcatttag ttactacgat tataaagctt
     1381 ataatttcaa tattgaaaaa acagactttg ttgtcatcca tattcccgat cagattggcg
     1441 atgctatggc catctttcct gttattcggg cgcttgaatt gcataaaatt aagcatcttt
     1501 taattgtaac gtcgacaatt aatttagaag tctttaatgc gcttaaactt gaacagacta
     1561 aattaacatt agtcacaatg actatgcagg atcacgcaac attaaaagaa ataaaagatt
     1621 tagcaaagaa cataacacag caatacggta cgccggatct ttgtattgag gggatgcgta
     1681 aaaagaacct gaaaacgatg ttatttatca gtcagttgaa agcaaaaacg aattttcagg
     1741 ttgttggtat aaccatgaat tgctactccc ctttgtgcaa gaacgcgtcc cgtatggatc
     1801 agaaactccg ggctcccgta cctatgacat gggcatttat gatgcgtgag gcgggttttc
     1861 cagcagtcag gccaatatat gaattgccac taagtgagga tgtactcgat gaggtgcgcg
     1921 aggaaatgcg atcgttagga tcttacattg cgttcaattt agaaggtagc tcgcaggaac
     1981 gtacattttc attatcgatt gcagaaaatc taatagcaaa aattcaaagt gaaacagata
     2041 taccaatagt gatcgttcat ggacccaaag gtgaagataa agccagggca ttagtggatt
     2101 gttataataa tgtctaccgt ttatccttac caccctcgat taaacgttca gcagcaatca
     2161 taaaagatgc ttatatcgca ataactcctg acacctcaat attacatatg gcaagtgcct
     2221 ataatacccc tgttgttgca atttatgctg attacaaaac gcgatggccc gcaatggccg
     2281 atgtctcgga gtcagtcgtc gttgggcaaa aaattgacaa tataagtctg gatgaattcg
     2341 caaaggcatt aaaaagtgtt ttggcgagaa tatgagacag tacttatgca tagtaatgcc
     2401 gatctgttat gaacggtggt gtcatcaata ctctgaggct ggtcaatcat cacagaaaac
     2461 cagcctttga atcaaagcag tactcaccgg atacgatcat agtcacatcg aacgcaattc
     2521 tggcggtgcc gcatttttat ttattcattt tctgtgagag aagggcaagc tctatagagg
     2581 gaatgtaaaa aagagagctc cgaatggagc actctttaag aaattataac ggaaaatcag
     2641 aggtttttat aacatgtcgc ataagcaaac ccaagctttt caaatacgct ttgatcacct
     2701 gttgccaggg aaactacgta ttgttcgctt gagtttgcgg
//
LOCUS       DAJTVK010000258         2726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000258 DAJTVK010000000
VERSION     DAJTVK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.264"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(423..680)
                     /locus_tag="OO372_004330"
     CDS             complement(423..680)
                     /locus_tag="OO372_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343641.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="OO372_004331"
     CDS             complement(677..1174)
                     /locus_tag="OO372_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCR6343642.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="OO372_004332"
     CDS             complement(1376..1834)
                     /locus_tag="OO372_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6343643.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="OO372_004333"
     CDS             complement(1831..2328)
                     /locus_tag="OO372_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6343644.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="OO372_004334"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="OO372_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCR6343645.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          707 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataac
//
LOCUS       DAJTVK010000259         2721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000259 DAJTVK010000000
VERSION     DAJTVK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.265"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..2546
                     /locus_tag="OO372_004335"
     CDS             30..2546
                     /locus_tag="OO372_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6343646.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="OO372_004336"
     CDS             2557..>2721
                     /locus_tag="OO372_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6343647.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAJTVK010000260         2720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000260 DAJTVK010000000
VERSION     DAJTVK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.266"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(265..465)
                     /locus_tag="OO372_004337"
     CDS             complement(265..465)
                     /locus_tag="OO372_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343648.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(465..737)
                     /locus_tag="OO372_004338"
     CDS             complement(465..737)
                     /locus_tag="OO372_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6343649.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(740..1018)
                     /locus_tag="OO372_004339"
     CDS             complement(740..1018)
                     /locus_tag="OO372_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343650.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(1019..1576)
                     /locus_tag="OO372_004340"
     CDS             complement(1019..1576)
                     /locus_tag="OO372_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343651.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPGKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT          759 a          535 c          507 g          919 t
ORIGIN      
        1 tcaattgata caccggaatc agcgaaagcc tgcaccatca tagcagtgtc gctatcaatg
       61 aaaatctgta attcatattc gttcattttt aaaatctcct ttttaccaga cctcctcaat
      121 cgctctgtaa cgagctaaac agtctttttc aggggtagcc tgtacagtgg tattacccac
      181 cctaaaataa gatagttttt tgcgttagaa tgcgattgag agcgtctgat aagctgtttt
      241 tctaatttct cttaaagtct cggactacca ataacctaat tccgctttat ttcgttcaaa
      301 ccattccgct gtaactgctg tctttagtgt tgcgtttggg ctggtggcat gaatgaagat
      361 atcttttgga ttgcaagcat caccgaaacc accttctttg atgagcaggt ctgatacatg
      421 cttaatcatt ttatctgtca aatattcgaa tttaatacat aacattaaat ctccaggagt
      481 aaattgtttg ggtggcaatt tcttttatta ccatccctga agataatctt tttagttgcc
      541 tgaacttcat gatgcaaaaa ccagatgaca tacttcagat aataatcacg agtgccataa
      601 cgaatcattg gattaccgta agcatcatct cttgtcttaa cctctccacc atcaatccat
      661 tcaagcattc ctgtctcata attaatcatg aagtaagttg caaagaaccg tttaatatca
      721 tctcgccatt gcttcataat cagacctcct gagacagttt gtgaattttg atacttagta
      781 accttgctaa atccagtcca aaagcattta aggatttcag actatcaagg tcatcttgct
      841 taatcctgat atggtgtctt gcagcctcta agcgactatt aagcaaatcc tgttcgttaa
      901 tatcacggtt aattgcaagc ttcatcactg ctgagtgtgg cagacctgta atgtttttga
      961 ttcggttcag ttcatcaaag gctgtctttg taactctggt taatgtgtgt ttattcattt
     1021 aaaaatcctc tgtaactaaa taggggtcaa actcgaaatc tggttgctcg ttttccattg
     1081 gctcaatatt cccaacggca atatgatgtt caacaatgcc aattaatttc gcgcacatca
     1141 cagcaagcaa agcgcaatat ttatcgcttg gtaattcgta actaagtctg gttgcctgtc
     1201 gctgaagatg atttgtcacg gtggatgcgt taatttcgtc aagattcttt agcagataaa
     1261 atagtttttt gttgtatttc gggtggcctt cacttctaca gaacccaata aaaacatcat
     1321 caagcttgcc tggtaactgc tcgtaactgt caaagttagc cagataattt ttaatagtat
     1381 tctttgttgt tttgcgtact ggcataaatc tgtctcgtgg cgtaatgcta cgaatgaccg
     1441 cttcaatgtt cttacgttcg gatttgctaa aggttgggac ttcgtagctg gtggggttgt
     1501 ttgtgtaagc gtaggagttg tgcttgttgc gttctcttga tgcctgaagc atcgctctaa
     1561 attctgaata ctgcattcat gttctgactc tcataagatg ttattattct gttaacctat
     1621 atacaatatt agcattttca aagactaatg tcaagtattt ttaacatttt cgtaacatct
     1681 gtggtataat gggtttatgg ggagagtaaa atatgaggcg tggctatgta tttggtcgca
     1741 tattttgcat tgcacacaga cctcttcaat cgccctgtaa cgagttaaac gacctcttca
     1801 ggtcaactca taaggtcagg tactttttgt gtcagaatgc gattgagagc gtcttacaag
     1861 gaaaactaat ggtaaatctc cctcctgagc tggggtcacc tgatgtatgg actggtggag
     1921 gtattgggct gattgcaggc ctgctacttc gctacgtcct cgtcagagtg cttgttgtct
     1981 ttaaactgtt cggtcgtaca tggacgcttg accttaagga taacgaccac acagaccccc
     2041 gttaaactcc ttcaaaaccc ctgcacgaga tgacagatag aagtctatgg tacaggggtg
     2101 tctttagcat tttgtgctat ctattaaaaa aatagcttga cagaaccgga aagccgtatc
     2161 tatcaagcat tcacctctct gacagtaatt tctaaaagtt cttatttttc aataagttgt
     2221 aattagccat atatagagaa ctaaaagatt ttttagctat tttgtttaaa agcatatcaa
     2281 acgatacatt ttattgattt aggtcaaaga caaaagcatt gacaaaatgg taagattgtt
     2341 cacatgacct tacttaacag acttttaaga gactttgaaa cagcctgttt aacaggtcac
     2401 tacctctggt cttatcgttc ccttctttct aggtggttat ctgttaagag tttttcagtt
     2461 tttattctta agataattgt taagatgata ctctcccttc gggttatata ggttacccct
     2521 aaaaatgggg ttaaaactcg ctacagagct ttctatgaga ttagcttcta atcgaatcaa
     2581 ctatgaggtt tggtaggatt gtgcaaaatc tgtacagtaa cttttcagaa tctgaaaatt
     2641 tttataaaaa atgactagcc ctctttgaat ctcatcttcc ccgaatcttc aaaaatatct
     2701 gacagatgtc tgtagctgct
//
LOCUS       DAJTVK010000261         2588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000261 DAJTVK010000000
VERSION     DAJTVK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.267"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            196..1149
                     /locus_tag="OO372_004341"
     CDS             196..1149
                     /locus_tag="OO372_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6343652.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OO372_004342"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="OO372_004342"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6343653.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2445..2576)
                     /locus_tag="OO372_004343"
     CDS             complement(2445..2576)
                     /locus_tag="OO372_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR6343654.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
BASE COUNT          715 a          637 c          577 g          659 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcgacaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 catacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatccccc
//
LOCUS       DAJTVK010000262         2576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000262 DAJTVK010000000
VERSION     DAJTVK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.268"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            544..1083
                     /locus_tag="OO372_004344"
     CDS             544..1083
                     /locus_tag="OO372_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR6343655.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(1309..1632)
                     /locus_tag="OO372_004345"
     CDS             complement(1309..1632)
                     /locus_tag="OO372_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR6343656.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1918..2112
                     /locus_tag="OO372_004346"
     CDS             1918..2112
                     /locus_tag="OO372_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6343657.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          836 a          626 c          549 g          565 t
ORIGIN      
        1 ttatcgccac tggcagcagc cactgataac cgaattagag gagtaagact tgaagtcatg
       61 cgcctgttaa ggctaaactg aaagaacaag ttttggtgac tgcgctcctc caagccagtt
      121 acctcggctc aaagagttgg tagctctgag aaccttcgaa aaaccgcctt gcagggcggt
      181 tttttcgttt acaggcagca gattacgcgc agaaaaaaag gatctcaaga agaaccttta
      241 attttttttc acacttggta tcctacttta acccataaaa taaacactta caccgacaca
      301 cccttaaaca aaagaaaaaa aacaataaaa aacaccgcaa aaatggattg ttaacaaatg
      361 aaaatactca attcgaatct cataacaaac atatcatcaa caataagaaa tcaccttcta
      421 acatcatcca aaaatataaa aacacaccca tcaccaaaca tggacgtaac aagtcatcgt
      481 cgtttattcc agaaagacgg aataggattg agccaaacag gaaaagcaac acaacttgtc
      541 attatgtcgc atggtggatg gaaagagatc tcccatccac aaacactatt cacgcgtcaa
      601 aaaggcgatg gatggactgt agtccccaaa aacctaagaa ttgattttta tacaaaagac
      661 aacgacttca caaagggact aagcgttctt tcagaggtaa acaaacgcca tactgaagct
      721 caaaaagggt taacaccatc actaaacata agcaaagatg acctaaaagt tctagcgaac
      781 gcgcgcaata tacctcaatc aaaattagaa gaagaaatga tgtcacaagc catatataga
      841 aaagaatacg caacaagtaa tgagaagata aaaaactatg ctctatatta ccatgaacaa
      901 gcccaaaata ttatacaaag acatcaagat ggcttaggaa acaaaaatat tgatatagca
      961 tttataactg ataaaaatca taagaagcac ctttctgaca tatttaaagt aataaatgaa
     1021 tcaggagtaa aatatgatgt aattcacttt ggcgcttgcc gagtaaatag agaggagaaa
     1081 taatcatcat ttaccataat cccttaattg tacgcaccac tgaaacgcgc tgaacgcgat
     1141 tctgacgcag acaccacaaa aatagatgtt ttaccccgga aaaacaggaa ggatgcgtca
     1201 ggatcgtttt cagctcgctg catagctatg catgaaagcg aatggagatc actttgggag
     1261 cttaccgtgt tcataccgtc tgttttcaac agttttctcc ccgggaagct aatctgccat
     1321 aagcctggat aacagggcac ggtgatactc cgtaatagcg atcagcacct ccgcatactc
     1381 cctgtccccg atacgcttgc cgtcgataaa gagttttttc tgtaacgccc ccagtctcat
     1441 aacttctttc agcacccgat catcagtcag tgagttaacg ttcttaccga gagctgccgc
     1501 ccgtaaaaaa ccagaaacgg tcttgccgca ttcagcagcc ctttttctga tttcctggtc
     1561 ttcttctgcc gtcagacgaa cagggcgact gatagcccgc ctgcgcgtat tacttccact
     1621 tcgttttgtc ataaaacatg gctccgtatc tgacacgggg tgtcggggcg aagccctgac
     1681 cagggtaatt gtaatagcgt gcatgtatgc gcggtataac aattacacat cctgtcctgt
     1741 cagcaagttc gaattaccga caaaggcacg gcagtttttt tggcagtgac agtattgtca
     1801 ggcagaaata cccgtcaatg cgtcagacgg cagaataccg gcggtaaaac agtcgaaaag
     1861 tggtacgcga tataatcaca caacgcagat gacagacaga acggagaaaa cagaaagatg
     1921 aacaagcagc agcaaaccgt actcaacatg gcgggattca taaaaagcca gagcctgacg
     1981 ctgctcgaaa agctggacgc actcgatgcc gacgaacagg ccgccatgtg tgagaagctg
     2041 cacgaaatcg cggaagaact ccagaacagc atacagacac gctttgaagc ggaaagtggc
     2101 accggggtat aacagtgtat cctgctccag acaggatgca gaacggtata ccggtgcgta
     2161 aggagaaaat gtcacataag gcgctcttcc gcttcctcgc tcactgactc gctacgctcg
     2221 gtcgttcgac tgcggcgagc ggtgtcagct cactcaaagg cggtaatgcg gttatccaca
     2281 gaatcagggg ataacgccgg aaagaacatg tgagcaaaaa gccgaaggca gggacaaagg
     2341 ccgcgcaggt ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2401 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2461 gaagctccct cgtgcgctct cctgttccga ccctgccgtt taccggatac ctgtccgcct
     2521 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcggc
//
LOCUS       DAJTVK010000263         2574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000263 DAJTVK010000000
VERSION     DAJTVK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.269"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..2413
                     /locus_tag="OO372_004347"
                     /pseudo
     CDS             1..2413
                     /locus_tag="OO372_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            2415..2574
                     /locus_tag="OO372_004348"
                     /pseudo
     CDS             2415..2574
                     /locus_tag="OO372_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          699 a          535 c          729 g          611 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggtaa
      961 ggggatagtt aatatttcaa cggacagtct ctggaattta aagacatcat ctactaacgc
     1021 acaattgcta caggtcggtg tattaggcac gggtgaactg aatattacca ccggaggtat
     1081 agttaaagcg cgtgatacac agatagctct caatgacaaa agtaagggcg acgtgagggt
     1141 ggatgggcag aactctcttc ttgaaacatt caatatgtac gtagggacat ctggtacggg
     1201 tacgttaacc ctgacgaata gcggcacgct gaatgtcgaa ggtggagaag tttacttagg
     1261 tgtttttgaa cctgctgtag gaacgctaaa cattggtgca gctcacggtg aggcggcggc
     1321 agatgccggg tatataacca acgcgacgaa agtagagttt ggttctggcg aaggtgtttt
     1381 tgtctttaat catactaata acagtgatgc cggctaccag gtcgatatgc tgattacagg
     1441 tgacgataaa gacggaaaag tgatccatga tgcaggccat acggtgttca atgcagggaa
     1501 tacttatagc ggtaaaacgc tggtcaatga cggcctcctg accattgcgt ctcatacggc
     1561 agatggggta acgggcatgg gttcgagtga agtcaccatt gcaagccccg gtacgctcga
     1621 cattctcgca tcaacgaaca gtgcaggaga ttacacgctg accaatgcgc tcaaaggcga
     1681 tggcttgatg cgagtgcagc tgtcatccta cgacaagatg tttggcttta cccatgcaac
     1741 agggactgaa ttcgccggtg ttgcccaact gaaagacaga accttcactc tggaacgcga
     1801 caacaccgct gcgcttactc acgcgatgtt gcagtctgac agtgaaaata ccacatcggt
     1861 aaacgtggga gagcaatcca ttggtggact ggccatgaat ggcggtacgc tcattttcga
     1921 tacggatatt cctgctgcga cgcttgcaga gggatatatc agcgtcgata cgctggttgt
     1981 cggcgcgggt gactacacct ggaaaggccg taactatcag gtaaacggga cgggcgacgt
     2041 gcttatcgac gtgcctaaac cgtggaatga tcccatagcg aataaccctc tgacgacgct
     2101 caatttgctg gaacacgatg atagccatgt cggcgttcaa ctggtgaagg cgcaaacggt
     2161 tattgggtcg ggtggctcat taacgttacg tgatttacag ggcgacgagg tggaagcgga
     2221 caaaacgtta cacattgcgc aaaacggaac ggtggtcgcc gagggtgatt atggattccg
     2281 cctcacgacc gcaccaggtg atggtttgta cgttaactat gggctgaaag cgctgaacat
     2341 ccatggtggg caaaagctga cgttagccga acatggcgga gcctatggcg caacggccga
     2401 tatgtcggca aaatagccat cgcttacacg accgtctggg tgagccgcag tatacagatt
     2461 cactgcattc tcagggatcg gcaagcagta tgtggatgcg tcatgtcggg gggcacgaac
     2521 gtttcaggac cggtgacggc cagctaaata ctcaggctaa ccgctatgta ttgc
//
LOCUS       DAJTVK010000264         2558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000264 DAJTVK010000000
VERSION     DAJTVK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.270"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(59..262)
                     /gene="tatE"
                     /locus_tag="OO372_004349"
     CDS             complement(59..262)
                     /gene="tatE"
                     /locus_tag="OO372_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6343658.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(391..1179)
                     /locus_tag="OO372_004350"
     CDS             complement(391..1179)
                     /locus_tag="OO372_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6343659.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1272..1655
                     /gene="crcB"
                     /locus_tag="OO372_004351"
     CDS             1272..1655
                     /gene="crcB"
                     /locus_tag="OO372_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6343660.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1709..1918)
                     /gene="cspE"
                     /locus_tag="OO372_004352"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1709..1918)
                     /gene="cspE"
                     /locus_tag="OO372_004352"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6343661.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2093..>2558)
                     /gene="pagP"
                     /locus_tag="OO372_004353"
     CDS             complement(2093..>2558)
                     /gene="pagP"
                     /locus_tag="OO372_004353"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6343662.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          667 t
ORIGIN      
        1 ttttgaagcg gtttttttgt atcgaacaag atattgaggg agcgtcctgc tcgccacgtc
       61 actctttatg agagagcttt tcagcctgaa gatcaacgtc tgcgcctttt ttcgccgcag
      121 catcgtcatc attcatcgcc ttcttgaacc ctttaatggc cgctccaagg tctccgccca
      181 gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag cgccgcaact accagcagtt
      241 tggtaatact aatctccccc atagatacct tcttgacata taaccggcaa cagaacgcgc
      301 cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc acacaatagc aatctgtaac
      361 aaggtgaatc aagcgccgag taaaaaaaca tcataataat tgcggcggcg caaagcgacg
      421 gttgtttaag acgggcagtt gcgcgcgcac ctgacgcaca cgttcgggcg tcacttccgc
      481 cataatgagt gcaggcattt ctgacgctgc cgcaatggtg acgccaaagg gatctataat
      541 ccggctttgc ccgatatttt tgttcccgca ctcccccgcc gccaccatat aacaggtggt
      601 atccagcgca cgagcggcaa gcaacgttga ccagtgatgc tctttgagcg gcccgcgaac
      661 ccaggcggca ggaagtacca ggatttcagc tccctgtaat gcctgtgcca gcgccagctc
      721 tggaaagcgt aagtcataac aggtcatcag accgaccttc atcccctcca cctcgagtaa
      781 cggagcgatt tcattaccag catcaacacg gcgtgattcc tgaatggcaa atgcatcata
      841 gagatgcaat ttggcataac gggcgacgat gtttcctgcc tgtagcgcca ccagcatatt
      901 ccatgcgcgc cccggcgttg aaggaacatg aatcgtcaga attgtcgtca tcatgttacg
      961 tttactttct cgccgtaaac gtccgaggaa ttcgccttcc agcagctgtg ctgatttaac
     1021 cgatagatct gcatcatgat catcgcgcgc cagcaatgct tccggcagga cgaacagcga
     1081 tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa atctcagcgt tcttttccca
     1141 cacagatgta acagcaaact gtcctgcggc aaccagcatt gccacctcct gtcagagtcg
     1201 taaaattcgc caacgacgat tattcggcgt tacacttatc actcatacaa atcaaatagc
     1261 aggattttgc agtgttacaa cttcttttag cagtttttat tggcggtggt acgggaagcg
     1321 tggcgagatg gctgttaagt atgcgattta acccgctgca tcaggcgatt ccgttgggga
     1381 cgctggcagc aaacctgatt ggggcattca tcataggaat gggattcgcc tggttcagca
     1441 ggatgacgaa cattgatcca gtgtggaaag tattaatcac caccggattt tgtggcggtc
     1501 taacaacctt ctcaacattt tcggcagaag tggtgttttt gttacaagag ggccgctttg
     1561 gctgggcgtt actgaacgtt ttcgtcaacc ttctggggtc ttttgccatg accgcactgg
     1621 ctttctggct gttttcggcc tcaaccgcac actaaagacc aaaaaaaacc cgctgattaa
     1681 gcgggttttg aattcttgct gacgtatctt acagagcgat tacgtttgca gcagaagggc
     1741 ctttggcacc gttagtgatt tcgaactcta cgcgctgacc ttcagcaaga gttttaaaac
     1801 cattagtctg tattgcagag aagtgtacga acacgtcttt gctgccgtct tccggagtaa
     1861 tgaaaccgaa tcctttggac tcattaaacc acttaacgtt acctttaatc ttagacatca
     1921 aaattacctt tacatgaaaa atagacacaa atgctgtgtc ggttaccagt acaccaattg
     1981 tggtacgctt tgtccagtgc aactttacta aaaagtgata aaagtcgcgt tatttttgat
     2041 gttacttatt ttagttattg attttaaaga agttactaaa acttcatttg tctcaaaact
     2101 gaaagcgcat ccaggcaaag tacacattgc cattgttgta ggtacccgga atgtaggtca
     2161 tctgaaaagt cactgggcca taacccacgg aggccaatgg cagtagaacc gggagaggga
     2221 tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa tcccagacct aaatgaaaat
     2281 tttcatccgc cagcggtcgc caggtacttt cccatccgta tccggcaatc ggttcccatt
     2341 tgttccacga gtccttaaat gccatggcat acaggccatg ccagtttcct ttttcatccc
     2401 atcgcgactg accaaaaccg ccaccccacg gtcgctcgtt atagcgatcg gttttttctt
     2461 tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat atataaatca taatgttcag
     2521 gctgttgcca ggtttgtgca atattttctc taaacgtt
//
LOCUS       DAJTVK010000265         2539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000265 DAJTVK010000000
VERSION     DAJTVK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.271"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="OO372_004354"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="OO372_004354"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6343663.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="OO372_004355"
     CDS             complement(1522..2031)
                     /locus_tag="OO372_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6343664.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          659 c          579 g          659 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccct
//
LOCUS       DAJTVK010000266         2472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000266 DAJTVK010000000
VERSION     DAJTVK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.272"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..713)
                     /locus_tag="OO372_004356"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="OO372_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="OO372_004357"
     CDS             complement(766..1362)
                     /locus_tag="OO372_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6343665.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="OO372_004358"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="OO372_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCR6343666.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2472)
                     /locus_tag="OO372_004359"
     CDS             complement(2006..>2472)
                     /locus_tag="OO372_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6343667.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          680 a          537 c          495 g          760 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagca gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 aggttgtcgc tt
//
LOCUS       DAJTVK010000267         2447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000267 DAJTVK010000000
VERSION     DAJTVK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.273"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(26..316)
                     /locus_tag="OO372_004360"
     CDS             complement(26..316)
                     /locus_tag="OO372_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6343668.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(306..743)
                     /gene="ratA"
                     /locus_tag="OO372_004361"
     CDS             complement(306..743)
                     /gene="ratA"
                     /locus_tag="OO372_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6343669.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            914..1396
                     /gene="smpB"
                     /locus_tag="OO372_004362"
     CDS             914..1396
                     /gene="smpB"
                     /locus_tag="OO372_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6343670.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1611..1973
                     /gene="ssrA"
                     /locus_tag="OO372_004363"
     tmRNA           1611..1973
                     /gene="ssrA"
                     /locus_tag="OO372_004363"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2172..>2447)
                     /locus_tag="OO372_004364"
     CDS             complement(2172..>2447)
                     /locus_tag="OO372_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR6343671.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          576 t
ORIGIN      
        1 tcaatgagca ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc
       61 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
      121 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
      181 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
      241 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
      301 caattttacc tagcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
      361 gacgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
      421 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
      481 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
      541 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
      601 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
      661 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
      721 gcggtccggc taatctgagg cataactatt tccatcaaca aaaatccaac aaataatatc
      781 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
      841 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
      901 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
      961 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
     1021 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
     1081 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
     1141 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
     1201 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
     1261 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
     1321 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
     1381 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
     1441 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
     1501 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
     1561 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
     1621 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
     1681 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
     1741 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
     1801 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
     1861 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
     1921 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccacttttga
     1981 caggactgca accggacaga gactataaaa acagtcactt acggacactg accagacagt
     2041 aagcggacca gaaaaagaca aaaatataca cgcaaaatgc acgtgcattt caaaaaagcc
     2101 tctgattatg aagtcagagg cttttctagt tgtaaataag ggtaacaatt taacacgatg
     2161 cagcttacca attacgtaac atcaaacgct ctaattgttc ccaaatcctc cagaccactc
     2221 agctcctctt tcatttcacg ctgacggcga taaatctcat cgttgcgatc gacctgcgcc
     2281 tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac ctgctgatta
     2341 tcggcatcac cccacgccat atgcgtttgt gctgtgacag attttgccag catgactacc
     2401 ggggacaggc ggcccagtga gtcggggcca gcattccaga tacgacc
//
LOCUS       DAJTVK010000268         2420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000268 DAJTVK010000000
VERSION     DAJTVK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.274"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            82..462
                     /locus_tag="OO372_004365"
     CDS             82..462
                     /locus_tag="OO372_004365"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR6343672.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            459..806
                     /gene="tnpB"
                     /locus_tag="OO372_004366"
     CDS             459..806
                     /gene="tnpB"
                     /locus_tag="OO372_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6343673.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            856..2394
                     /locus_tag="OO372_004367"
     CDS             856..2394
                     /locus_tag="OO372_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6343674.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
BASE COUNT          561 a          675 c          708 g          476 t
ORIGIN      
        1 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
       61 gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc
      121 cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata
      181 tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga
      241 caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca gctacttcct
      301 gtgactctcg atgcagctgc cgaacagcca gaatcgctcg cagaggaccc gaaaaccctc
      361 agtatcagct gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc
      421 gaaaagctcc tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga
      481 tcaaaatttg gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg
      541 cgaaagtaca gacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg
      601 gccgcagcgg cagtcaggtt aaactgctgt ggtccaccag tgacggactg tgcctcctga
      661 ccaaacggct ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc
      721 ttacacaggc gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc
      781 tgctgacctc cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg
      841 gtaaaatacg ggcttatgaa cgacatctct tctgacgaca tcttcctgct gaaacagcgc
      901 ctggccgaac aggaagcgct gatccacgcc ctgcaggaaa agcttagcaa ccgggagcgc
      961 gaaatagacc atctgcaggc gcagctggat aaactccgcc ggatgaactt cggcagtcgt
     1021 tccgaaaaag tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcagaaa
     1081 gagagcgata cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag
     1141 acccgcaccc gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct
     1201 gcggcgccgt gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccgcc
     1261 gaacagctgg agttgatgcg tagcgccttc cgggttatcc ggacggtacg ggaaaaacat
     1321 gcctgtactc agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg
     1381 ggtatcgccg gaccggggct gctggcccgc gtgctgacct cgaagtatgc agagcacacc
     1441 ccgctgtatc gccagtcaga aatatacggc cggcaaggtg tggagctgag ccgttcactg
     1501 ctgtcgggct gggtggatgc atgctgccgg ctgctgtctc cgatggaaga ggcgcttcat
     1561 ggctatgtgc tgactgacgg taagctccat gttgatgaca cgcctgtccc ggtgctgttg
     1621 ccaggcaata agaaaacgaa gaccgggcgg ttgtgggcgt atgttcgtga cgaccgtaac
     1681 gccgggtcaa cgctggcgcc ggcggtgtgg ttcgcttaca gcccggacag aaaaggcatc
     1741 catccgcaga cccatcttgc ggggttcagt ggtgtactgc aggcggatgc atacgccggg
     1801 ttcaacgagc tgtaccggga tggccggata acggaagccg cctgttgggc tcacgcccgc
     1861 cgtaaaatcc acgatgtgca cgttcgcacc ccgtcagccc tgacggagga agcgctgaaa
     1921 cggatcggcg aactgtacgc catcgaggca gagataaggg gaatgacggc ggaacagcgc
     1981 cttgccgaac gtcagttgaa aacgaaaccg ctgctgaaat ccctggaaag ctggctgcgt
     2041 gaaaagatga aaaccctgtc gcgacactca gaactggcga aagcgttcgc atacgccctg
     2101 aaccagtggc cggcgctgac gtactatgca gatgatggct gggctgaggc ggacaataac
     2161 atcgctgaaa atgcgttgcg gatggtcagt ctgggccgca aaaactacct gttcttcggt
     2221 tcggatcatg gaggagagcg gggagcgctg ctgtacagcc tgatcgggac gtgcaaactg
     2281 aacggagtgg agccagaaag ctacctccgc tatgtccttg acgtcatagc cgactggccg
     2341 ataaaccggg tcggcgaact gctcccctgg cgcgtagcac tgccgactga ataacacatt
     2401 cccgtcaata cggttcttgc
//
LOCUS       DAJTVK010000269         2403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000269 DAJTVK010000000
VERSION     DAJTVK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.275"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="OO372_004368"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="OO372_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCR6343675.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="OO372_004369"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="OO372_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="OO372_004370"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="OO372_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          667 a          504 c          618 g          614 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagtcc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 cta
//
LOCUS       DAJTVK010000270         2300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000270 DAJTVK010000000
VERSION     DAJTVK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.276"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            20..238
                     /locus_tag="OO372_004371"
     CDS             20..238
                     /locus_tag="OO372_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6343676.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="OO372_004372"
     CDS             240..605
                     /locus_tag="OO372_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6343677.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="OO372_004373"
     CDS             602..1267
                     /locus_tag="OO372_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343678.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="OO372_004374"
     CDS             1267..1632
                     /locus_tag="OO372_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6343679.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="OO372_004375"
     CDS             2087..2242
                     /locus_tag="OO372_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6343680.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          623 a          485 c          621 g          571 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac
//
LOCUS       DAJTVK010000271         2286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000271 DAJTVK010000000
VERSION     DAJTVK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.277"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..325
                     /gene="casB"
                     /locus_tag="OO372_004376"
     CDS             <1..325
                     /gene="casB"
                     /locus_tag="OO372_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCR6343681.1"
                     /translation="VSANVKAIDERQPFAAQLAAVMSKGRFTRLSAVKTPDELLRQLR
                     RAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRIRWALEYYQAGDAD
                     NEQNQ"
     gene            337..1392
                     /gene="cas7e"
                     /locus_tag="OO372_004377"
     CDS             337..1392
                     /gene="cas7e"
                     /locus_tag="OO372_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCR6343682.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1403..2149
                     /gene="cas5e"
                     /locus_tag="OO372_004378"
     CDS             1403..2149
                     /gene="cas5e"
                     /locus_tag="OO372_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR6343683.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2131..>2286
                     /gene="cas6e"
                     /locus_tag="OO372_004379"
     CDS             2131..>2286
                     /gene="cas6e"
                     /locus_tag="OO372_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311637.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR6343684.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREEL"
BASE COUNT          569 a          570 c          613 g          534 t
ORIGIN      
        1 agttagcgcc aatgttaaag ccattgatga acggcagcct tttgccgcgc aactggcggc
       61 ggtaatgtca aaggggcgtt ttactcgcct gtcagcagta aaaacaccgg atgaattact
      121 gcgccagtta cgccgcgccg tcagattatt aaacggttcc gtcaatcttg actccctggc
      181 agatggcatt ttccgctggt gccaggagag cgacgacctg ctcaatcatc accgccgcca
      241 gcagcggcca accgaattta tccgtatccg ctgggcgctg gaatattacc aggctggcga
      301 cgccgataac gaacaaaatc aataaaggac tatctcatga caacatttat tcagcttcat
      361 gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg acaccggtgc gccaaaaact
      421 gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc aaagtctgaa acgtgcgtgg
      481 cgcacttctg cactttttga acaggcactg gcgggccata ttggtattcg cagtgggcgt
      541 attgcgcgtg aggcggcaac tatcctgatt gagaaaggga ttgaagataa aaaagccatc
      601 gaatgggcgg caaaaattgc tgattatctt gggaaagcta aaaatgacaa aaaaccaaaa
      661 gatccgctca ctaacgccga aactgaacaa ttagttcata tcagcccggc agaatttgac
      721 gccgtaaaag cgcttgctca tcaactggca gaagaaaagc gcgcgccaaa agaggaagat
      781 ctcgcgttgt tacgtaaaga ccgtatagcg gtagatatcg ctatgtttgg tcgtatgctg
      841 gcgaataaac ctgagtttaa tgttgaagcc gcctgccagg tcgcgcatgc gtttggtgtc
      901 agtgaaacga ttgtcgaaga tgattttttc accgccgttg atgatttgcg ccaggcctct
      961 gaagatgccg gtgccgggca tctcggagaa accggattcg gttcagcact tttctacacc
     1021 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     1081 aatcagacca tacgcgcttt tacggaagcc gcgcttaaag tttccccaac cggtaaacaa
     1141 aatagctttg ccagccgtgc ctatgcttcc tgggcgctgg cagaaaaagg caccgatcaa
     1201 ccacgctctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     1261 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     1321 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     1381 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     1441 ggcgtcctgg ggtgtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     1501 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     1561 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     1621 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     1681 ccgtcgtgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     1741 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     1801 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     1861 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaacgcgt gtgatgcctt
     1921 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     1981 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     2041 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     2101 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     2161 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     2221 cagtggctgt gggatctttt ccctggcggc aaagaaaggc aatttcttta tcgtcgggaa
     2281 gaactc
//
LOCUS       DAJTVK010000272         2232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000272 DAJTVK010000000
VERSION     DAJTVK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.278"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..1004)
                     /locus_tag="OO372_004380"
                     /pseudo
     CDS             complement(1..1004)
                     /locus_tag="OO372_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
     gene            1077..>2232
                     /locus_tag="OO372_004381"
     CDS             join(1077..1326,1326..>2232)
                     /locus_tag="OO372_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6343685.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPKASPITPEQIEIRKLRKKLQRIEMENEIFKKGYRALDVRLPEQFSII
                     GKLRAHYPVVTLCHVFGVHRSSYRYWKNRPEKPDGRRAVLRSQVLELHGISHGSAGAR
                     SIATMATRRGYQMGRWLAGRLMKELGLVSCQQPTHRYKRGGHEHVAIPNYLERQFAVT
                     EPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVGCAMSFSPDSRLTMKALEMAWETR
                     GKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWM
                     PVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNSVA"
BASE COUNT          583 a          552 c          560 g          537 t
ORIGIN      
        1 gaccaataca tatttgattg aaaaccagct tcagtcgtgt aatgccccat atctccccat
       61 tcactgtaca gtgacccgat gcctcgggaa ctactgtggt ttaaatagtt tgctctgggg
      121 agcgtgtagc cattgctgct gcaaaaattt tcagcaaggc tgtatatcat gaaagcctcg
      181 ccggcgttca cccaccaact cttcacacgg atttcgtata catagctagg gcctcctgat
      241 tttggtgtcg ccgtaatcct ttccgaattg ctgccgacat ttttaaatgt cactttaccg
      301 gtagcatcaa catcgaccca ggaggcagag cttgaaaact cataatcagc cgccgttttt
      361 cctggggcaa agttgtcatt attcaactga taatacgccc cggtgaaccc ctgcgaaggg
      421 aatgtagttg tcggtaggtc agtaccattg actgatactg taccgctcat tattttgtct
      481 tctgcgcgag tgaattgtat tgtggtactc agaccagctt gatgaacacc attcaataca
      541 gggatcagcg ttgtgatacc ctctccgcct cctgtaacag tggctttgta ctcgcctgag
      601 aaatttttac tgtagtcaat tgcactaact tgcacatagg gcacgttggt acctgactgt
      661 acaaattcca gcccttcaga aactttgatc ggattgcctg atatatcgtg cagaacaaga
      721 tgtagctcag cactatcggt aaagtctcct ttcaatgatg accgattgtt cttaaggact
      781 gactttgagg cgtctgctgg gcctgccacc agcgttatat ctactgtttg cattccacca
      841 gcggtcgtga ctgcgcttac cgtgacgcta cctgtcatcg ctcctttcac gcttgttgtc
      901 gcgattccgt tttgatcggt caccgctgtt aatgacgtta atgtggcaga gccgcttttt
      961 aaggcaaagt acacagtgag accttcgatc aggttaccac tgcctgaagt ggcacactga
     1021 atttggccac ctgaacagag gtgatatgct cacctcagaa caacacaggt gctccaatga
     1081 aaaaaagaaa ttttagcgca gagtttaaac gcgaatccgc tcaactggtt gttgaccaga
     1141 aatacacggt ggcagatgcc gccaaagcta tggatgttgg cctttccaca atgacaagat
     1201 gggtcaaaca actgcgtgat gagcgtcagg gcaaaacacc aaaagcctct ccgataacac
     1261 cagaacaaat cgaaatacgt aagctgagga aaaagctaca acgcattgaa atggagaatg
     1321 aaatattaaa aaaggctacc gcgctcttga tgtcagactc cctgaacagt tctcgataat
     1381 cgggaaactc agagcgcatt atcctgtggt cacactctgc catgtgttcg gggttcatcg
     1441 cagcagctac agatactgga aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt
     1501 acgcagtcag gtacttgagc tacatggcat cagccacggt tcggccggag caagaagcat
     1561 cgccacaatg gcaacccgga gaggctacca gatgggacgc tggcttgctg gcaggctcat
     1621 gaaagagctg gggctggtca gctgtcagca gccgactcac cggtataaac gtggtggtca
     1681 tgaacatgtt gctatcccta actaccttga aaggcagttc gccgtgaccg agccaaatca
     1741 ggtgtggtgc ggtgatgtga cctatatctg gacgggtaag cgctgggcgt acctcgccgt
     1801 tgttctcgac ctgttcgcaa gaaaaccagt gggctgtgcc atgtcgttct cgccggacag
     1861 caggctcacc atgaaagcgc tggaaatggc atgggaaacc cgtggtaagc ccggcggggt
     1921 gatgttccac agcgatcagg gcagtcatta tacgagcagg cagttccggc agttattgtg
     1981 gcgataccag atcagacaga gtatgagccg gcgcggaaac tgctgggata acagcccaat
     2041 ggaacgcttc ttcaggagtc tgaagaacga atggatgccg gtggtgggtt acgtaagctt
     2101 cagcgaggca gctcacgcca taacggacta tatcgttgga tattacagcg cactaagacc
     2161 gcacgaatat aacggtgggt tacccccaaa cgaatcggaa aatcgatact ggaaaaactc
     2221 taactcggtg gc
//
LOCUS       DAJTVK010000273         2157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000273 DAJTVK010000000
VERSION     DAJTVK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.279"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            6..2157
                     /gene="rhsC"
                     /locus_tag="OO372_004382"
                     /pseudo
     CDS             6..2157
                     /gene="rhsC"
                     /locus_tag="OO372_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          506 a          659 c          663 g          329 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga atggcgaaac cgcagagcgg tggcagtatg
      961 acgaacgcgg ctggctgaca gacatcagcc atctcagcga agggcaccgg gtgacggtgc
     1021 attacgggta tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catcacccgc
     1081 agacggaagc actgctctgg cagcatgaga ccagacatgc gtacaacgcg caggggctgg
     1141 cgaaccgctg tataccggac agcctgtccg ccgtggaatg gctgacctac ggcagcggct
     1201 ggctgtcagg catgaaactc ggcgacacac cgctggtgga gtacacccgc gaccgcctgc
     1261 accgggaaac gctgcgcagc ttcggccgtt atgaactcac catcgcttat acccctgccg
     1321 ggcagttaca gagccagcac ctgaacagcc tgctgtctga ccgcgattac acctggaacg
     1381 acaacggcga actcatccgc atcagcagcc cgcgccagac ccggagttac agctacagca
     1441 ccaccggcag gctgaccggc gttcacacca ccgcagcgaa tctggatatc cgcatcccgt
     1501 atgccacaga cccggcgggg aaccgcctgc ccgacccgga gctgcacccg gacagcaccc
     1561 tcagcatgtg gccggataac cgtatcgccc gtgacgcgca ctatctttac cggtatgacc
     1621 atcacggcag actgacagag aaaaccgacc tcatcccgga aggggttatc cgcacggatg
     1681 atgagcgcac ccatcggtac cattacgaca gtcagcaccg gctggtgcac tacacgcgga
     1741 cacaatatgc agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg
     1801 tggcaaaacg ggtatggcgg cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga
     1861 aaccgcaagt gacctggtac ggctgggacg gcgaccggct gaccacaata cagaacgaca
     1921 gaacccgcat ccagacgatt tatcagccgg ggagcttcac gccactcatc agggtcgaaa
     1981 ctgccaccgg tgagctggcg aaaacgcagc gccgcagcct ggcggatgcc cttcagcagt
     2041 ccggcggcga agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc
     2101 ggctggaaag tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctgg
//
LOCUS       DAJTVK010000274         2101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000274 DAJTVK010000000
VERSION     DAJTVK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.280"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(60..1025)
                     /locus_tag="OO372_004383"
     CDS             complement(60..1025)
                     /locus_tag="OO372_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR6343686.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="OO372_004384"
     CDS             complement(1891..>2101)
                     /locus_tag="OO372_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343687.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          582 a          551 c          439 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAJTVK010000275         2098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000275 DAJTVK010000000
VERSION     DAJTVK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.281"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            91..1263
                     /gene="istA"
                     /locus_tag="OO372_004385"
     CDS             91..1263
                     /gene="istA"
                     /locus_tag="OO372_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HCR6343688.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1263..2060
                     /gene="istB"
                     /locus_tag="OO372_004386"
     CDS             1263..2060
                     /gene="istB"
                     /locus_tag="OO372_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HCR6343689.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          556 a          505 c          593 g          444 t
ORIGIN      
        1 gtgatataag acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct
       61 taaggttctc acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata
      121 aagcaaatgc gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct
      181 gaacggacgg tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac
      241 aaaatggtta agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc
      301 tggaatagcg aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc
      361 atgctgcgtt actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc
      421 ttcgaaactc agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt
      481 gccgggcaac ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc
      541 catgtcttcg ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc
      601 ttccgctact tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg
      661 ctgaagaata acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac
      721 tataacttcc tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag
      781 cggatggtga aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc
      841 actcatgtca atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt
      901 cgccagttca aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg
      961 ttaccggata cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc
     1021 tatatcgagg ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta
     1081 tcgatacgaa tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc
     1141 tcacatcgcc tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg
     1201 ctctggcagc aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg
     1261 tgatgcatga actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg
     1321 ttgaaagcct gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca
     1381 tggcgctaca gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc
     1441 aggctcgtct gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca
     1501 tcgaccgtaa ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg
     1561 tgatcctgct gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga
     1621 aagcggtgga tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac
     1681 tgatgaaagc gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc
     1741 gggtgttgat cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt
     1801 tcttccggct actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag
     1861 ggttcgcaga ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc
     1921 ggctgctaca tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga
     1981 aacgtaaagc tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg
     2041 aaagcggaca gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAJTVK010000276         2096 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000276 DAJTVK010000000
VERSION     DAJTVK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.282"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..126)
                     /locus_tag="OO372_004387"
     CDS             complement(<1..126)
                     /locus_tag="OO372_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6343690.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYT"
     gene            387..722
                     /gene="iraM"
                     /locus_tag="OO372_004388"
     CDS             387..722
                     /gene="iraM"
                     /locus_tag="OO372_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6343691.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1003..1134)
                     /locus_tag="OO372_004389"
     CDS             complement(1003..1134)
                     /locus_tag="OO372_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR6343692.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1634..1710)
                     /locus_tag="OO372_004390"
     tRNA            complement(1634..1710)
                     /locus_tag="OO372_004390"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1674..1676),aa:Arg,seq:tct)
     gene            complement(1811..1886)
                     /locus_tag="OO372_004391"
     tRNA            complement(1811..1886)
                     /locus_tag="OO372_004391"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1851..1853),aa:Ile,seq:cat)
     gene            complement(1989..>2096)
                     /locus_tag="OO372_004392"
     CDS             complement(1989..>2096)
                     /locus_tag="OO372_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6343693.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          372 g          525 t
ORIGIN      
        1 ggtataaggg gttatggcca ccggactacc aaacggctgc cagccctctt tcagtttatg
       61 tgtcagcttt tccgcaagat ctgacggcgg cgccgccctg acaacatcat agtgtttaaa
      121 tgccatggtt ctttccacca tctgaaaaat aattctttaa aatacctgac atgtaataca
      181 gaaaaaacac aaaaccatac cttaaataaa aacctgatta tcaagcagat atgcatggat
      241 aaactacaag acgagatata aaccaccctg catttaaata aacaataaac aacatcagaa
      301 aaataattct gctctatggt ttacattcaa aaatatcatt tatacttttc agaacatcac
      361 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      421 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
      481 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
      541 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
      601 ggaattcaat caaaaatagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
      661 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
      721 aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga
      781 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
      841 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
      901 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
      961 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt
     1021 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccag
     1081 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1141 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1201 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1261 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1321 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1381 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1441 atattcactc tacccacacc atatgaatga taaatattta atgctgaaaa aaccattaga
     1501 ccgtataaca aacactcaat caatacttaa cagaactttt atttttgaca aacataaaat
     1561 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     1621 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     1681 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     1741 cccgcccccg caccaacaac cctgttatcg tgtcgtctgc tcttcctgat aagctaatgg
     1801 cggtttgtga tggtggccct tgctggattt gaaccagcga cctggcgatt atgagtcgct
     1861 cgctctcacc actgagctaa agggccggga gcagaataat aacggtccgt aattaattcc
     1921 gcaataaaaa acccgctcgg cggcgggttg tagaaactct tctaacgtca ggcataaaac
     1981 gcccatagtt atgacgaatt taccacagat tccggaaaaa tcaacctagt tacctttttt
     2041 aactgccgct cagcccatgc ttcttcaata tcaaaccgag tcaccagcgc atcata
//
LOCUS       DAJTVK010000277         2005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000277 DAJTVK010000000
VERSION     DAJTVK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.283"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..389
                     /locus_tag="OO372_004393"
     CDS             <1..389
                     /locus_tag="OO372_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343694.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO372_004394"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="OO372_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6343695.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="OO372_004395"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="OO372_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6343696.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="OO372_004396"
     tRNA            complement(1806..1881)
                     /locus_tag="OO372_004396"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="OO372_004397"
     tRNA            complement(1890..1966)
                     /locus_tag="OO372_004397"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAJTVK010000278         1955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000278 DAJTVK010000000
VERSION     DAJTVK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.284"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1138
                     /locus_tag="OO372_004398"
     CDS             <1..1138
                     /locus_tag="OO372_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6343697.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="OO372_004399"
     CDS             1150..1599
                     /locus_tag="OO372_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343698.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          603 a          320 c          526 g          506 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttactcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggcgacat gacccgcagg gaaagatcat cagaagttca
     1741 ctttttgtac taaataattc gcattttatg tttaaaaatt gagatattcc ttattaccta
     1801 aagctgtttt ttattgctta tacatgatca aatacttctt acataattaa ggagaacaaa
     1861 atggaactta aaaaattgat ggaacatatt tctattatcc ccgattacag acaagcctgg
     1921 aaagtggaac ataaattatc ggatattcta ctgtt
//
LOCUS       DAJTVK010000279         1884 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000279 DAJTVK010000000
VERSION     DAJTVK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.285"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            76..345
                     /locus_tag="OO372_004400"
                     /pseudo
     CDS             76..345
                     /locus_tag="OO372_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            460..630
                     /locus_tag="OO372_004401"
     CDS             460..630
                     /locus_tag="OO372_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343699.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(757..1314)
                     /locus_tag="OO372_004402"
     CDS             complement(757..1314)
                     /locus_tag="OO372_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6343700.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1311..1586)
                     /locus_tag="OO372_004403"
     CDS             complement(1311..1586)
                     /locus_tag="OO372_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6343701.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1771..1884
                     /locus_tag="OO372_004404"
                     /pseudo
     CDS             1771..1884
                     /locus_tag="OO372_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          528 a          387 c          457 g          512 t
ORIGIN      
        1 ttttcattga tttcaccatg ttatagtttt atttgtgaat taaatcaatt atggcgatga
       61 attacaaggg gttaaatgct gccgcagcat agcgatattg aaatagccta gtatgcttcg
      121 atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt
      181 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
      241 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      301 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      361 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      421 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      481 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      541 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      601 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      661 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      721 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      781 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      841 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      901 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      961 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
     1021 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
     1081 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
     1141 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1201 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1261 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1321 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1381 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1441 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1501 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1561 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1621 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1681 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1741 tcagatacaa tgtcttattg cattttttaa aacatgtcag tgtggtacat ggatatcgat
     1801 accatcgcca attataaact gggcggtgca cagcaacatg atagcgtacg cctcgatccg
     1861 tgggtgttta tgttctcagc agga
//
LOCUS       DAJTVK010000280         1861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000280 DAJTVK010000000
VERSION     DAJTVK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.286"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(89..643)
                     /locus_tag="OO372_004405"
     CDS             complement(89..643)
                     /locus_tag="OO372_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6343702.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="OO372_004406"
     CDS             complement(640..930)
                     /locus_tag="OO372_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR6343703.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="OO372_004407"
     CDS             complement(930..1529)
                     /locus_tag="OO372_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR6343704.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="OO372_004408"
     CDS             complement(1601..1813)
                     /locus_tag="OO372_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343705.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          461 a          496 c          378 g          526 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 a
//
LOCUS       DAJTVK010000281         1819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000281 DAJTVK010000000
VERSION     DAJTVK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.287"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="OO372_004409"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="OO372_004409"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6343706.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="OO372_004410"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="OO372_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1819)
                     /locus_tag="OO372_004411"
     CDS             complement(1450..>1819)
                     /locus_tag="OO372_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6343707.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          479 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgca
//
LOCUS       DAJTVK010000282         1770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000282 DAJTVK010000000
VERSION     DAJTVK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.288"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..739)
                     /locus_tag="OO372_004412"
     CDS             complement(<1..739)
                     /locus_tag="OO372_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR6343708.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDI"
     gene            complement(880..>1770)
                     /gene="metG"
                     /locus_tag="OO372_004413"
     CDS             complement(880..>1770)
                     /gene="metG"
                     /locus_tag="OO372_004413"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HCR6343709.1"
                     /translation="VNADIVNKVVNLASRNAGFINKRFDGVLASELADPQLYKTFTDA
                     AEVIGEAWESREFGKAVREIMALADLANRYVDEQAPWVVAKQEGRDADLQAICSMGIN
                     LFRVLMTYLKPVLPKLTERAEAFLNTELTWDGIQQPLLGHKVNPFKALYNRIDMKQVE
                     ALVEASKEEVKAAAAPVTGPLADDPIQETITFDDFAKVDLRVALIENAEFVEGSDKLL
                     RLTLDLGGEKRNVFSGIRSAYPDPQALIGRHTIMVANLAPRKMRFGISEGMVMAAGPG
                     GKDIFLLSPDAGAKPGHQVK"
BASE COUNT          409 a          459 c          422 g          480 t
ORIGIN      
        1 caatatcaat ctgttgaagg tgtattatta catcagtagc atattccaga ccgccttcct
       61 ggacgatctg atccataaca tcctcacgca ctttatatct tgtgcatttc atttcaagtg
      121 ccgtgagtac agccattact ggtgatggta actgtgttgt tttcaggccg tcgatctgac
      181 tgatcgcctg ggcgacggtg ttttgccatt ctaaagaaca ggcgctgaag tcaaatgtgg
      241 ctgttttatt atcccgttgc gacacactac aggctaagcg acgtaattgg gtcaatttgt
      301 ctgcttgctg aacaggaact ccgggaaagc ggcgatgact taacgtggtg tgtacaactt
      361 cgggcggaaa aacgacagca atatctttcg ccagccacgg taaaacagca tctgtatttt
      421 tgtcaggcgc acgggtttct gctacgaaag agggttgttc ctgaataatg gtggataagt
      481 ccgctacctt gagagagccc gttaccgttt gggaaggttg caaagaatta tccttcgctt
      541 gctccacata ccccttcttc accttctccg caatcagctt aagctccgct tttgccgctg
      601 ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact tttccccaac
      661 tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat ttttcgtcct
      721 gatagataaa gtgtctcatt ctgcatccct gtaattttat ccagttgaat gcagatgcta
      781 ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt aggaatttat
      841 aaagaaaagg cgctgtcgat gcagcgcctt gaagagggat tatttcacct gatggcctgg
      901 tttagcacca gcatccgggc ttaacaggaa gatatctttc ccgccaggac cagcagccat
      961 taccatgcct tcggagatac caaagcgcat tttacgcggt gccaggttgg ccaccataat
     1021 ggtgtgacgc ccgatcagtg cctgcggatc cgggtaggca gaacgaatac cggagaagac
     1081 attgcgtttt tcaccgccga gatccagtgt caggcgcagc agtttgtcag aaccttcaac
     1141 aaactctgcg ttttcgatca gcgccacgcg caggtcaact tttgcgaagt cgtcaaaggt
     1201 gatggtttcc tgaatcggat catctgccag tgggccagta actggcgcgg cagctgcttt
     1261 cacttcttct ttagacgctt ccaccagtgc ttcaacctgc ttcatatcga tgcggttata
     1321 cagcgctttg aacggattca ctttgtggcc cagcagcggt tgctggatac catcccaggt
     1381 cagttctgta ttgaggaatg cttctgcgcg ctcggtcagt ttcggcagta ccggcttcag
     1441 gtaagtcatc agcacgcgga acaggtttat gcccattgag caaattgcct gcaggtcggc
     1501 atcgcggcct tcctgttttg ccaccaccca cggagcctgc tcgtcgacat agcggttagc
     1561 cagatcagcc agcgccatga tttcgcgcac tgctttacca aattcgcggc tttcccacgc
     1621 ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt cagccagttc
     1681 gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacgag aggccaggtt
     1741 aaccactttg ttcacgatat cggcattcac
//
LOCUS       DAJTVK010000283         1768 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000283 DAJTVK010000000
VERSION     DAJTVK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.289"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1517
                     /gene="tssI"
                     /locus_tag="OO372_004414"
     CDS             <1..1517
                     /gene="tssI"
                     /locus_tag="OO372_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6343710.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQ
                     TVGVNLVETVGSNQIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQV
                     GLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKD
                     GSIFLNGTHIHLEGESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1593..>1768
                     /locus_tag="OO372_004415"
     CDS             1593..>1768
                     /locus_tag="OO372_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6343711.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVL"
BASE COUNT          452 a          425 c          559 g          332 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc
     1021 tggtggaaac ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg
     1081 tggggatcat tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta
     1141 tgaatacctc ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg
     1201 tgggaatggg ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga
     1261 tgaaggaaaa caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct
     1321 gtggtaaggc aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca
     1381 ttcatctgga aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg
     1441 gtgccacaca acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg
     1501 atatgcggca attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga
     1561 tgaaccatat taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc
     1621 agggcgacat gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag
     1681 gtgcgccgac gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg
     1741 tgaacccggt gctgggtgcg aaggtgct
//
LOCUS       DAJTVK010000284         1753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000284 DAJTVK010000000
VERSION     DAJTVK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.290"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(50..193)
                     /locus_tag="OO372_004416"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="OO372_004416"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="OO372_004417"
     CDS             complement(197..1018)
                     /locus_tag="OO372_004417"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6343712.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1753
                     /locus_tag="OO372_004418"
                     /pseudo
     CDS             1007..1753
                     /locus_tag="OO372_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="OO372_004418"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          448 a          391 c          413 g          501 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc aca
//
LOCUS       DAJTVK010000285         1739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000285 DAJTVK010000000
VERSION     DAJTVK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.291"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(34..1092)
                     /locus_tag="OO372_004419"
     CDS             complement(34..1092)
                     /locus_tag="OO372_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HCR6343713.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1200..1440)
                     /locus_tag="OO372_004420"
                     /pseudo
     CDS             complement(1200..1440)
                     /locus_tag="OO372_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1665..>1739)
                     /locus_tag="OO372_004421"
     CDS             complement(1665..>1739)
                     /locus_tag="OO372_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6343714.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          417 a          509 c          380 g          433 t
ORIGIN      
        1 gcataataac gacgcgtaat taattcttca atatcatccg ttctggctga ctaaatcctg
       61 tacttcccga accgtctgct caaaacgttc agtctccagc tcaacgccaa ttgcacgacg
      121 cccgagcgcc agtgccgctt tcactgtcga acccaacccc atgaaaaaat ctgcaatcag
      181 gtcacccgga cgactgctag cgctgattat ctgctgcagc atttctgccg gtttttcgca
      241 cggatgtttc ccgggataga actgcaccgg tttatgcgtc cacacatcgg tatacggcac
      301 ctgcaccgtc acgccaaaat accgccgcag atgtttatat tcactcagca gctccgcata
      361 ctgccggttc agtgaggtat acgtatccac cagctggtgg tggagctttt ccagttcacc
      421 acgccgatgc ttctcttctg ccacccgggc aaacagcgcc tgtaatttca gataatcgct
      481 ttcattcggc aactgccact gaccggcact gaaccagtgc gacaccatgt ttttctttcc
      541 tgtggcatct gcaatctgtt ttgccgttat ccccagggca gctcgcgcat cacgaaagta
      601 agaaatcagc ggggccatca catgctgttt cagtgcactg cccttcgccg catacccggc
      661 atctttcgga cgatacggcc cctgataatg ttccgcgaac agaatgcgct ctgtggcggg
      721 gaaatacgcc cgcaggcttt ccttgttgca tccgttccag cgtccggacg gcttcgccca
      781 gataatatgg ttcagcacac tgaagcgttc acgcatcatg atttcgatat cagatgccag
      841 gcgatgacca cagaacaggt aaagacttcc ggcaggtttc agcacccgcc agaactgcgc
      901 cagacactgg tccagccact tcaggtaatc atcgtcgccc ttccactggt tatcccagcc
      961 ctcaggcttc actttaaagt acggcgggtc cgtgactatc aggtcaacag aattttcggg
     1021 taacgaccgg ataaattcca ggcagtcggc gttgattaac tcacaactgg atatttttac
     1081 agtattaagc atggatcatt aagcctgtct ctgataggct cattctgctt ttgcgcaaag
     1141 cagtgggcct gaggtttgct tgtgaaccca acgcatgagc agatggctgg tgggtgcccc
     1201 taacacccac cagccgccca tttaccacaa ataaaaaagc cttcactgcg gaaggcgtct
     1261 gtaacaaccg aactgatagt ctgccagacc cgccataacc agctgggtca gtattaactg
     1321 gcagcgttcg cgtgaaaggt aagtattctg cgctatctcc ccgactgtcg ccggttcggt
     1381 aacgcttaat tcattaaaca ccactctggc ggtttctgtc atatcctgct gttttagcat
     1441 gtcttttcct tttccggtta acgtgacaca ccaataactc ttgtcgaaaa agccagcaag
     1501 ctgaaagaca ggtattcacc gccaccagcg cgtttactgt actgacgcga tttcagtcat
     1561 aaaaaacccg ctcggcggcg ggttgtagaa actcttctaa cgtcaggcat aaaacgccca
     1621 tcgttatgac gaatttacca cagattccgg aaaaatcaac cttgttacct agttaccttt
     1681 tttaactgcc gctcagccca tgcttcttca atatcaaacc gggtcaccag cgcatcata
//
LOCUS       DAJTVK010000286         1686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000286 DAJTVK010000000
VERSION     DAJTVK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.292"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(784..1455)
                     /gene="queE"
                     /locus_tag="OO372_004422"
     CDS             complement(784..1455)
                     /gene="queE"
                     /locus_tag="OO372_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR6343715.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            1594..>1686
                     /locus_tag="OO372_004423"
     CDS             1594..>1686
                     /locus_tag="OO372_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343716.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
BASE COUNT          551 a          343 c          301 g          491 t
ORIGIN      
        1 atcgcactct atcaattttc acttaaagta tgaactggat atctttattc ataagaacta
       61 ttcatcaaca gcgtatagag gcggttatgt aaaaccactc gttagcctca aaacaaagac
      121 aaatttccag atttattact taatcaatga ataacaagct taaaaaacaa actattaacc
      181 taaagaaaaa attaacagac attaattcaa tattaaaaaa ataatagatt aaaatttctt
      241 aacgatttaa gaatcataca aataacactt tgattaaatt taattttttg tatcgataat
      301 tgtgaacctc tctggcatgg agaactattt tgaacatgag gtgttacgtg gatattttgc
      361 ttattacaag tactgctaat ataaaaactt gagaaagaga taacgggtta tatggtggtt
      421 tatccccgct ggcgcgggga acactctaaa cataacctat tattaattaa tgatttttta
      481 agccagtcac aatctaccaa ctttacagta tcacacaaac aacacatcca ttatgttaaa
      541 gagtacttaa ttcattgatt aaaaaggtaa atatttaaaa taactctata caaattaaaa
      601 tctaccaaac tttaccgcaa taattttcac tccagcgaaa aattaatgcc acagaatttg
      661 tagagaatgc taatgattag ccctggatgg gtaaaatgga ggagttttca gaggcgacaa
      721 acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct tataaatgtt
      781 taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat tacgcgcaat
      841 gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct gcagtgcaat
      901 gactcgcggt ttatcatcgg tcagcgttgc caatagttca tccagtgctt caatatcgcg
      961 tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg acaacacgtc
     1021 atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg gtgtgcagcg
     1081 tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt cgagcagatc
     1141 agtcagtggt agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca catgccgcgc
     1201 ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg ccccccactt
     1261 atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat cctcaagctt
     1321 ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct gtaaacgaat
     1381 aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga acatctcgtt
     1441 aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt gcagatcctg
     1501 ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa aaatgcgaaa
     1561 catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa agaaaatgga
     1621 tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt agtttttaat
     1681 gataac
//
LOCUS       DAJTVK010000287         1678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000287 DAJTVK010000000
VERSION     DAJTVK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.293"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..890)
                     /locus_tag="OO372_004424"
     CDS             complement(<1..890)
                     /locus_tag="OO372_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6343717.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPT"
     gene            complement(990..1346)
                     /locus_tag="OO372_004425"
     CDS             complement(990..1346)
                     /locus_tag="OO372_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6343718.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1482..1589)
                     /gene="rprA"
                     /locus_tag="OO372_004426"
     ncRNA           complement(1482..1589)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO372_004426"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          334 c          393 g          413 t
ORIGIN      
        1 gtgggatgca ggcagacggt aaacagagca ataaatgaga taaaggtgta cagcatcagt
       61 aatgttgttg ggcgaacggt attacgaatg agtggagcgg taataaatac acaaagcagc
      121 gaacccacgg tgtagatact gagtagtttg atcgacatag tgtatgacat gcctgcaaca
      181 aattgtccgt actgtgccag ccactggcta accagataaa acgttgccat tgagatatag
      241 ccatataagg tataactggc taaatcgata attgaacagc gatgttccgt agagctggtg
      301 gttttcttta tgacaggtaa gcgacgaccc ggatggggtg ggaacgtaca acggtataaa
      361 aacagagcgt taataaacat aatgcctgca gcaatcataa aggaccaacc gaaccacagt
      421 tcagcccaca ctaacaggct aatgattagc ggtaataaaa attgtccgct ggaaacaaat
      481 gctttaatta aaatattggc tgtcccaggt gagcgtggaa aagcttccat caaactggga
      541 taagtgcctg catcgagaaa actgtttgcc attcccgcca gaaagccaaa aacataagcg
      601 ataatgatgt tattggtctg caggatgcca aaaaagaagg ccatatagca gcacatcccg
      661 agcatgataa aagggcggcg accaaagcga tcggataata atcctgcaaa aagcaagaca
      721 cttaatcgac caatgcccag cgatgagata actatcgaga cacccgcggc attagtctgc
      781 caaagtgtct ccagcgaggc catattcagg ctcatcaaaa tgacgcccat accatgcacc
      841 aggtaattaa aatacaaccc aagtgcggta gggtaatagg gatttttcat aggttctact
      901 tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag tatccattca ttattgttgt
      961 aacaacgaca attcctttct gtggaaaaat taacaaagcc gctccagatc gtgtgcatat
     1021 aattcagcgg ctaccgattg atagatcttc cagtcaataa aattgccatc agggtagact
     1081 tgttgaaacg cggttaagtc ggggaaaaaa cgcatggtgt tgttgccgat gcggttattt
     1141 attttctcta tgatggcatg aagatgtttt tttcgttgct gacgcatagc caacagttgc
     1201 tcgactacac aatcaggaat ggcgcatttg ccattttccc actgacgcca gctttcactg
     1261 tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag tcatggcaaa aatatggcgg
     1321 agtgcctgga gttcataagc gttcatcgtg taatggctgc atgaaataaa caataaaata
     1381 taaataacat tatcagcctg ctgacggctt gaagagagtc acagtatctt gtgcaacatt
     1441 attggaaaga gtgaggggcg aggtagcgaa gcggaaaaat gttaaaaaaa agcccatcgt
     1501 gggagatggg caaagactac acacagcaat tcgttgtttc actcagggga tttccatgct
     1561 tataaatcaa tatgttgatt tataaccgtg agctaatagt aggcatacgg actttttgcg
     1621 tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa tttgcgacag gtactacc
//
LOCUS       DAJTVK010000288         1559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000288 DAJTVK010000000
VERSION     DAJTVK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.294"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            273..1388
                     /locus_tag="OO372_004427"
                     /pseudo
     CDS             273..1388
                     /locus_tag="OO372_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          615 a          281 c          221 g          442 t
ORIGIN      
        1 caaaaaaaat cacttcacac actatcagag aacataataa ctttttaaaa tgagcgcatt
       61 ttgatagttg ttaacgattt cctttacgat actatcacta cccttttttt atacacaaat
      121 attaccgggt ggagataaaa gggaaatcaa aggtaattat atcaggaaga tataacgcat
      181 tacatttatt gtgtgtagaa gaaaatccat tcctgttcta attatccaat ttaaacatct
      241 tagcataaaa caaatgatga ataaggaatt ttatgtcaat tgactttacc ccaggtataa
      301 taaatacata tcacggcgat atttataact gcacaacaaa taccgataat gctaaaacgc
      361 cagacacacc gaagtggcct tgcgataatt gggaagaaca acaacccatt aattccacat
      421 tctctggaga aggatatatc tctgatcaat atgatttagt gcaacaccaa cttcaacaaa
      481 ttaatgcgtg ccacacgaac actacatata caaatgcaga ctactcaaaa gttgtggctc
      541 aacttgtaag tcttattact aacattgaaa cgataagttc gacacaactt acgcaacaga
      601 cacaatcaat acttaatcag attaataaca ttagatatga aaaaaacaaa agtgctgaat
      661 gtcgaatcat tgttgtcgct aatcctaaac ccgacaaggc aatcataact aaaattagcg
      721 tagaagaggg aatacccata acattcagtg tccaaaccat gttttcagat actaatttta
      781 ttgctgaaca acgagctgac ttaccaacta atataaaaga tattcaaagc ctgtatcaaa
      841 aaatgacgaa gttatatatt gagcacagtg aaaataaaaa caggatgaaa gtctttgccg
      901 gagccaactt tattgatttc aatatgacag gacagaacct gtccgggttc gttctaacct
      961 tgtcaaggtt ttatttcgaa gatctactta atataaattt cactgacgca aatttaggtg
     1021 acactatttt ttacataaag aacaccctac ccccaaatta tataaagatg gacaatatct
     1081 tgacaaacaa atcgaaggtt tattttcaac attattaacc attaatgaca actttttacg
     1141 agcaaaagcg gaaattgctt cgacaataat taaattttta gaggccagga taaccaatct
     1201 ttcatacaat gacatattaa aatatcagca agaattccaa aaacaatgtt ataagcaagt
     1261 taaagccttt acgacactct ctcgatacaa taaaattcaa acatgggcag aaatgtctga
     1321 atatcagttc gaagtatttc aatacgagac gcttaacccc aaaaaaatgt cacacacgcc
     1381 ttatttaaag aggccacttc ctaatgaaaa agatataaat tatggggtgg aaatagaaat
     1441 accatctggc aaaagaattc gcttatcaaa tcattatcag aatataatac cataatattg
     1501 attattagca ctttataatt attgaatgaa aatagatttt atgtaatttt aaaacaaag
//
LOCUS       DAJTVK010000289         1516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000289 DAJTVK010000000
VERSION     DAJTVK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.295"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>1516)
                     /locus_tag="OO372_004428"
     CDS             complement(<1..>1516)
                     /locus_tag="OO372_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6343719.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAJTVK010000290         1461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000290 DAJTVK010000000
VERSION     DAJTVK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.296"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            503..958
                     /gene="csgB"
                     /locus_tag="OO372_004429"
     CDS             503..958
                     /gene="csgB"
                     /locus_tag="OO372_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6343720.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            999..1451
                     /gene="csgA"
                     /locus_tag="OO372_004430"
     CDS             999..1451
                     /gene="csgA"
                     /locus_tag="OO372_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR6343721.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          447 a          275 c          336 g          403 t
ORIGIN      
        1 aaatgataga aaagttgtac atttggtttt tattgcacaa ttttaaaaaa tcatacaaat
       61 ggtgataact tactaataat gcatataaaa aatatttcgg tgtagtcctt tcgtcatgta
      121 aaaagttgtt gttttttctc cacacctccg tggacaaatt tttactccaa aaagacgagg
      181 tttgtcacgg cttgtgcgca agacatatcg cagcaatcag cgatgggcaa gaagaatgac
      241 tgtctggtga tttttgatag cggaaacgga gatttaaaag aaaacaaaat attttttgcg
      301 tagataacag cgtatttacg tggcttttaa tactttggta tgaacaaaaa aagaaaaata
      361 caacgcgcgg gtgagttatt aaaaatattt ccgcagacat actttccatc gtaacgcagc
      421 gttaacaaaa tacaggttgc gttaacaacc aagttgaaat gatttaattt cttaaatgta
      481 cgaccaggtc cagggtgaca acatgaaaaa caaattgtta tttatgatgt taacaatact
      541 gggtgcgcct gggattgcag ccgcagcagg ttatgattta gctaattcag aatataactt
      601 cgcggtaaat gaattgagta agtcttcatt taatcaggca gccataattg gtcaagctgg
      661 gactaataat agtactcagt tacggcaggg aggctcaaaa cttttggcgg ttgttgcgca
      721 agaaggtagt agcaaccggg caaagattga ccagacagga gattataacc ttgcatatat
      781 tgatcaggcg ggcagtgcca acgatgccag tatttcgcaa ggtgcttatg gtaatactgc
      841 gatgattatc cagaaaggtt ctggtaataa agcaaatatt acacagtatg gtactcaaaa
      901 aacggcaatt gtagtgcaga gacagtcgca aatggctatt cgcgtgacac aacgttaatt
      961 tccattcgac ttttaaatca atccgatggg ggttttacat gaaactttta aaagtagcag
     1021 caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct cagtacggcg
     1081 gcggtaacca cggtggtggc ggtaataata gcggcccaaa ttctgagctg aacatttacc
     1141 agtacggtgg cggtaactct gcacttgttc tgcaaactga tgcccgtaac tctgacttga
     1201 ctattaccca gcatggcggc ggtaatggtg cagatgttgg tcagggctcg gatgacagct
     1261 caatcgatct gacccaacgt ggcttcggta acagcgctac tcttgatcag tggaacggca
     1321 aaaattctga aatgacggtt aaacagtttg gtggtggcaa cggtgctgca gttgaccaga
     1381 ctgcatctaa ctcctccgtc aacgtgactc aggttggctt tggtaacaac gcgaccgctc
     1441 atcagtacta atacatcatt t
//
LOCUS       DAJTVK010000291         1457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000291 DAJTVK010000000
VERSION     DAJTVK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.297"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OO372_004431"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="OO372_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR6343722.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1437)
                     /locus_tag="OO372_004432"
     CDS             complement(1090..1437)
                     /locus_tag="OO372_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HCR6343723.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          406 a          320 c          364 g          367 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg tagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg atcatcaggg cagtcatttt
     1441 cactgcagcc agcagca
//
LOCUS       DAJTVK010000292         1436 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000292 DAJTVK010000000
VERSION     DAJTVK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.298"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            89..>1436
                     /gene="istA"
                     /locus_tag="OO372_004433"
     CDS             89..>1436
                     /gene="istA"
                     /locus_tag="OO372_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HCR6343724.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKY"
BASE COUNT          367 a          401 c          364 g          304 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg cgcagaacgc ttccgcatgc tggatgcacc
     1021 agcactctct ccgctgcctc tggagcctta tgaatacacg gaatacaaag ccgtgaaggt
     1081 tggccctgac taccacgttg aatatgcccg tcactggtac tccgttccac acgaactggt
     1141 cggtcagcgt ctgtcactga aggtggggca gtcggtggta cagctctggc acaaggggca
     1201 gtgtgtggcg caacatccgc gcagcacaca tgaatataaa cacacaacca atccgcttca
     1261 catgccagaa cgccaccggc ggcatgggac atggacaccg gaacgcctta tcgagcaggg
     1321 aaaccgtaca ggtccgtcca caggccgggt ggttgaatcc atgctgaaag ccaaacccca
     1381 tccggagctg gcttaccgtg cagtcctggg tctgctggca cttcagaaaa aatacg
//
LOCUS       DAJTVK010000293         1431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000293 DAJTVK010000000
VERSION     DAJTVK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.299"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            13..438
                     /locus_tag="OO372_004434"
     CDS             13..438
                     /locus_tag="OO372_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6343725.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            463..>1431
                     /locus_tag="OO372_004435"
     CDS             463..>1431
                     /locus_tag="OO372_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6343726.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLD"
BASE COUNT          386 a          332 c          341 g          372 t
ORIGIN      
        1 tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc
       61 gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg
      121 aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc
      181 cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg
      241 atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat
      301 gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat
      361 tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg
      421 ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata
      481 gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc
      541 agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag
      601 tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt
      661 aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac
      721 gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc
      781 aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc
      841 tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac
      901 atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg
      961 ctcaccatca actacgattt ttctggttca catacactac gttcagatta tggttcacaa
     1021 gagacagata ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta
     1081 cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc cattagtacc
     1141 tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga tacgtggacg
     1201 gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat
     1261 atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc aaagtccaac
     1321 gccaccgtca tcattcggca gaatggctac gtgatttatc agtctgccgt tccacaaggt
     1381 gcttttgaga tcaccgatct caacaccgcc agtacaggtg gcgatttgga c
//
LOCUS       DAJTVK010000294         1426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000294 DAJTVK010000000
VERSION     DAJTVK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.300"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..118)
                     /locus_tag="OO372_004436"
     CDS             complement(<1..118)
                     /locus_tag="OO372_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6343727.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO372_004437"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="OO372_004437"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6343728.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="OO372_004438"
     CDS             complement(822..>1426)
                     /locus_tag="OO372_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6343729.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAJTVK010000295         1392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000295 DAJTVK010000000
VERSION     DAJTVK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.301"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..459
                     /locus_tag="OO372_004439"
     CDS             <1..459
                     /locus_tag="OO372_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6343730.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="OO372_004440"
     CDS             477..1115
                     /locus_tag="OO372_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6343731.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAJTVK010000296         1376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000296 DAJTVK010000000
VERSION     DAJTVK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.302"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            3..983
                     /locus_tag="OO372_004441"
     CDS             3..983
                     /locus_tag="OO372_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR6343732.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="OO372_004442"
     CDS             998..>1376
                     /locus_tag="OO372_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6343733.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAJTVK010000297         1344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000297 DAJTVK010000000
VERSION     DAJTVK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.303"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..477)
                     /gene="umuC"
                     /locus_tag="OO372_004443"
     CDS             complement(<1..477)
                     /gene="umuC"
                     /locus_tag="OO372_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6343734.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
     gene            complement(477..896)
                     /locus_tag="OO372_004444"
     CDS             complement(477..896)
                     /locus_tag="OO372_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6343735.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1269..>1344
                     /locus_tag="OO372_004445"
     CDS             1269..>1344
                     /locus_tag="OO372_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HCR6343736.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALD"
BASE COUNT          405 a          321 c          277 g          341 t
ORIGIN      
        1 ctgccgctgc catttttttg ccgcatgatt ggcaagctta gccagcgttt tggtctgggc
       61 gatccccata ccaacagtaa gatgggtacg ttgtagcacc gttgcgcgaa tttctctgcc
      121 aaaatcagtc agatcgcgac aattacgcac acctgtcaga tcgcagaatg cctcatcaat
      181 actgtaaatc tcgacgcggg gcgatagttc ttccagcgtc gacatcaccc gattgctcat
      241 gtctgcgtaa agctcataat tgctgctaaa gcaaaccaca ccacagcgac gaaacagatc
      301 tttttgtttg aaccagggat cgcccatttt aacacccagc gcctttgcct cagcgtttcg
      361 ggcgataacg caaccgtcat tattcgatag cacaaccacc ggtttacccc ataaatcagg
      421 gcgaaacacc gtctcacagc tggcataaaa cgcgtttaca tcacagagag caaacatcag
      481 cgcatcgcct taacgacgtg gatcaccaca ccaaagacat ccagcgtatc ttcgctactg
      541 atggtaatgg gcgagtacgc actgttcatg ggaataagct gtaccgtcgg gcgtagttgc
      601 aattttttca ccgtaaactc gccgtcaaca gcagcgatga caatatcacc atggctggcg
      661 gtaatagcgc tatcgacaat cagtaaatca ccgtcactaa ttccaccatc aatcatagaa
      721 tcaccacttg ctttgacgaa gtaagtcgcg ctgggatgct ggatcaacag ttgattcaga
      781 tcgatgcgct gttcgacgta atctgctgcc ggtgaaggaa agccacactg aacaagatcg
      841 ctaaatagcg gaaaagtcac aatttcgcgg agatccgcag gcttgataaa caacataata
      901 atctgcctga agttatactg tttttatata cagtaatctg ttcttgccag cagatcaata
      961 ctgattcagg ctatcaatat ttgtcgctgc atagactgct gatttttcgt tctcttatcc
     1021 tgtgctcacg tggccttctg gcgacgacgc tcatccagca gaaatgaaaa atatcacccg
     1081 gcaaaaaaat agaatagaag cattcgccat aatgacatta aacattgttt gatatttatc
     1141 atattaatag aaataaagac attgacgcat cccgcccggc taactatgaa ttagatgaag
     1201 taaaatttat taatagttgt aaaacaggag tttcattaca atttatatat ttaaagaggc
     1261 gaatgattat gactgaaatc gttgcagata aaacggtaga agtagttaaa aacgcaatcg
     1321 aaaccgcaga tggagcatta gatc
//
LOCUS       DAJTVK010000298         1343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000298 DAJTVK010000000
VERSION     DAJTVK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.304"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..186
                     /locus_tag="OO372_004446"
     CDS             <1..186
                     /locus_tag="OO372_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343737.1"
                     /translation="ATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLA
                     QGKRRCQQQFTSQEVQN"
     gene            196..>1343
                     /gene="yicI"
                     /locus_tag="OO372_004447"
     CDS             196..>1343
                     /gene="yicI"
                     /locus_tag="OO372_004447"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6343738.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          319 c          352 g          337 t
ORIGIN      
        1 gccacgatta gcatcattat tgcgctattc acgattgttc cggcgatctg ttatttgctg
       61 agcgcgatta tcgctaaacg ctactactca ctcacgacgc acaatctgaa aaccgttatg
      121 gaacagctgg ctcagggtaa acgccgttgc cagcaacaat tcacctctca agaagtgcag
      181 aactaaggaa cggcaatgaa aattagcgat ggaaactggt tgattcaacc tggcctcaat
      241 ttgattcacc cgcttcaggt gttcgaggtt gaacagcagg ataatgaaat ggtggtctat
      301 gctgcccccc gtgatgtgcg tgaacgtacc tggcagcttg atacgccttt atttacgctg
      361 cgctttttct ccccacagga aggtattgtc ggtgtgcgga ttgagcattt tcagggggcg
      421 ctgaataacg gtcctcatta tccgctcaat attttgcagg acgtgaaggt cacaatcgaa
      481 aacacagaac gttatgctga gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt
      541 gagttctggt cactggattt tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa
      601 aataatggct acgtgcagga cacgaataat caacgcaatt atatgtttga gcggcttgat
      661 cttggcgttg gcgaaacagt ttacggtctg ggagagcgct ttactgccct ggtgcgcaat
      721 ggccagacgg tagagacctg gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa
      781 aatatcccgt tctacatgac taaccgtggt tatggggtac tggtcaatca tccccagtgt
      841 gtctcttttg aagtgggatc ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa
      901 tatctcgaat actttgttat cgacggcccg acgccgaaag cggtacttga tcgttatacc
      961 cgctttactg gtcgtccggc gctgccgccc gcgtggtcct tcggcctgtg gctaaccact
     1021 tcatttacca ccaactacga cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa
     1081 cgcaatctgc cgctgcatgt tttccacttt gactgtttct ggatgaaagc cttccagtgg
     1141 tgcgattttg agtgggaccc gctgactttc cctgacccgg aagggatgat ccgccgcctg
     1201 aaagcgaaag ggctgaaaat ctgcgtctgg attaacccct atatcggtca aaaatccccc
     1261 gtctttaaag agttacaaga gaaaggctat ttactcaaac gcccggacgg ttcgctatgg
     1321 cagtgggata aatggcagcc agg
//
LOCUS       DAJTVK010000299         1305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000299 DAJTVK010000000
VERSION     DAJTVK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.305"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            192..>1305
                     /locus_tag="OO372_004448"
     CDS             192..>1305
                     /locus_tag="OO372_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6343739.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQA"
BASE COUNT          277 a          411 c          347 g          270 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      781 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      841 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      901 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      961 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
     1021 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
     1081 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
     1141 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
     1201 tcaaacgggc gttgttcctg tcggccttcg ccgcgctcag ggatccgctc tccagggctt
     1261 actacacccg caaaatgagt cagggaaaac gacacaatca ggcgc
//
LOCUS       DAJTVK010000300         1294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000300 DAJTVK010000000
VERSION     DAJTVK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.306"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1112
                     /locus_tag="OO372_004449"
                     /pseudo
     CDS             1..1112
                     /locus_tag="OO372_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAJTVK010000301         1282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000301 DAJTVK010000000
VERSION     DAJTVK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.307"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            132..1151
                     /locus_tag="OO372_004450"
     CDS             132..1151
                     /locus_tag="OO372_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR6343740.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAJTVK010000302         1264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000302 DAJTVK010000000
VERSION     DAJTVK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.308"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(23..>1264)
                     /locus_tag="OO372_004451"
     CDS             complement(23..>1264)
                     /locus_tag="OO372_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6343741.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          334 a          298 c          227 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtca ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAJTVK010000303         1262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000303 DAJTVK010000000
VERSION     DAJTVK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.309"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            62..563
                     /gene="tnpA"
                     /locus_tag="OO372_004452"
                     /pseudo
     CDS             62..563
                     /gene="tnpA"
                     /locus_tag="OO372_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(581..1249)
                     /locus_tag="OO372_004453"
                     /pseudo
     CDS             complement(581..1249)
                     /locus_tag="OO372_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          331 a          289 c          282 g          360 t
ORIGIN      
        1 ctcctgttga ccacctggtc ttaactggaa tttaaatgcc tgtaatcgtt tcacaggtca
       61 aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc attgtgtttt
      121 cctgatgcat gttcacctgg tctttgtcac tagataccga cgccagattt tttgacgcga
      181 cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct gaactggttg
      241 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctaacctccc aaactggcga
      301 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      361 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      421 gtagctgcgg aggtgtgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      481 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggatggggt tttacggcgc
      541 accggataaa aagcgccatc taacgatggc gcgtctggaa ctactttttg ttaacgtcaa
      601 acatggtggc atcggtcgcc atgtcatcaa taacttgttt aatattttcg aaagccattg
      661 gcgtactttc gttattcagg tctttacctt cgccctgacg aacgactttg ataaccggtt
      721 tattagtcgc tgcatcaatc agctcacctt caaaatagag tcgagtatcc atggtacggt
      781 ggcctgtagc catttgcgtc cccgcaacca ctaatgcaac aggaaccact tcatagaatt
      841 gcagcccttc tttgctggta tctacaccgg taatggcccc acggaaaatc agactacgcg
      901 gcccagcggt ggtaaccagt ggtttacgct gcgctatcgc ttctttcatt tcggtgttgg
      961 tatagttcaa aattttatcc agaactttct gccctaccta ggtagaaggt tttggcaccg
     1021 gataataagt gattgggttc cagacgatgc tgtcatattt gctttgatca aaactcgggt
     1081 ctacccaacg taaaacaggt ttacctgttg ctgaggttgt ttcttttaaa tcagagtaat
     1141 tgtttaaaaa accagaatat ttatctggct gggtgatttt agaggcacaa ccagacagag
     1201 ccaacaagcc gcataaagct gcaactttcg caaatgatgt ggtacgcatg aatagtttcc
     1261 tg
//
LOCUS       DAJTVK010000304         1260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000304 DAJTVK010000000
VERSION     DAJTVK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.310"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..164)
                     /locus_tag="OO372_004454"
     CDS             complement(<1..164)
                     /locus_tag="OO372_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HCR6343742.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSA"
     gene            complement(487..1260)
                     /locus_tag="OO372_004455"
                     /pseudo
     CDS             complement(487..1260)
                     /locus_tag="OO372_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          408 a          189 c          250 g          413 t
ORIGIN      
        1 gcgctaaata gcatccagac acagaaggca agaagtagac aactgactga tatccagagg
       61 tttcttcgag caatatgttt tcctttattt tcccagaagg ccggattttc tggtttccag
      121 tcgcgtaaaa gataacgact atttttctca ttttgcagtg ccatattgtt cctcacatgc
      181 acacattggt aatgaaaaaa gacaaaacag gaggtaaggc gcaatagcca gttattagaa
      241 ttaaggatga attgggtgaa gtgctgattg aaagaataga taagaaagcg taaccgcggg
      301 ggcagaatgg ggattaagta gccagatatg tgttactaaa tgtaactaac aagctaacct
      361 tcaaacgggg ttaatctttg aacgtatgtc atgtttcaac ccttcagatc gtgaatctaa
      421 agggttacat attaactata ttatcaacaa gaccagacac ttatagaatt agtgatgatt
      481 tattcactaa aaaggccatg aactggagga aaacgatctg tccagaaggt ctatcggttc
      541 ctctcttatt gcctgattgt aataatcgtt attacagtaa gtattgttta tttcatctaa
      601 ggtcatgttt ttaactttta tattcctctg gtttacaact tcctctgtct tgtaagtgtg
      661 tatattatat ggggtatatg ctatttttaa taggatagtg aaacaattcg ttatccacat
      721 ctattccgtc agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa
      781 gcttaagtgt tggggaaaac aaattaaata acatgagctg catagtaggg caattgttct
      841 tcccagaaac atttaaaaaa tttaagtttt tgctctgttt tattatggga aataaagttc
      901 caatatcgga tgtattatta gtatcaatgt gggttatttt attgttgtcc agtcttaaac
      961 ttttaataga tggaagacaa gtcaggaaat caagatttat atttttcaac tggttgttag
     1021 cagcgtttaa tgtctgaaca ttgcagaagt tgctatagaa tattttaatt tcagaaagca
     1081 ggtttttgtt aataagtaac gtattcaagt tgggaaataa gacattgtgt aaacttgtta
     1141 gtttattatg ggatagatcc agcagtaaga gttaattttt attacctgca acaatatcag
     1201 ataattgatt atggctgaga ttcaggtatt gcagacattc gcaagattct aattgcacaa
//
LOCUS       DAJTVK010000305         1248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000305 DAJTVK010000000
VERSION     DAJTVK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.311"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1248
                     /gene="rhsC"
                     /locus_tag="OO372_004456"
     CDS             <1..>1248
                     /gene="rhsC"
                     /locus_tag="OO372_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HCR6343743.1"
                     /translation="VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAH
                     RHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGL
                     KRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLD"
BASE COUNT          294 a          367 c          386 g          201 t
ORIGIN      
        1 tggtactgac cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg
       61 ggacggaacc gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca
      121 acggcatccg tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac
      181 ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg
      241 accgcagcaa tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg
      301 tggcgcaccg tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg
      361 tgacagaaca gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca
      421 tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc
      481 tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg
      541 cggtgggcag gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg
      601 atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
      661 actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc
      721 gcaaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc
      781 gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca
      841 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg
      901 gttatgtaac ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg
      961 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
     1021 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
     1081 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca
     1141 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
     1201 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacc
//
LOCUS       DAJTVK010000306         1218 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000306 DAJTVK010000000
VERSION     DAJTVK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.312"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>1218
                     /locus_tag="OO372_004457"
     rRNA            <1..>1218
                     /locus_tag="OO372_004457"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          328 a          267 c          384 g          239 t
ORIGIN      
        1 ccggagaatg ttatcacggg agacacacgg cgggtgctaa cgtccgtcgt gaagagggaa
       61 acaacccaga ccgccagcta aggtcccaaa gtcatggtta agtgggaaac gatgtgggaa
      121 ggcccagaca gccaggatgt tggcttagaa gcagccatca tttaaagaaa gcgtaatagc
      181 tcactggtcg agtcggcctg cgcggaagat gtaacggggc taaaccatgc accgaagctg
      241 cggcagcgac gcttatgcgt tgttgggtag gggagcgttc tgtaagcctg cgaaggtgtg
      301 ctgtgaggca tgctggaggt atcagaagtg cgaatgctga cataagtaac gataaagcgg
      361 gtgaaaagcc cgctcgccgg aagaccaagg gttcctgtcc aacgttaatc ggggcagggt
      421 gagtcgaccc ctaaggcgag gccgaaaggc gtagtcgatg ggaaacaggt taatattcct
      481 gtacttggtg ttactgcgaa ggggggacgg agaaggctat gttggccggg cgacggttgt
      541 cccggtttaa gcgtgtaggc tggttttcca ggcaaatccg gaaaatcaag gctgaggcgt
      601 gatgacgagg cactacggtg ctgaagcaac aaatgccctg cttccaggaa aagcctctaa
      661 gcatcaggta acatcaaatc gtaccccaaa ccgacacagg tggtcaggta gagaatacca
      721 aggcgcttga gagaactcgg gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga
      781 aggcacgctg atatgtaggt gaagtccctt gcggatggag ctgaaatcag tcgaagatac
      841 cagctggctg caactgttta ttaaaaacac agcactgtgc aaacacgaaa gtggacgtat
      901 acggtgtgac gcctgcccgg tgccggaagg ttaattgatg gggtcagcgc aagcgaagct
      961 cttgatcgaa gccccggtaa acggcggccg taactataac ggtcctaagg tagcgaaatt
     1021 ccttgtcggg taagttccga cctgcacgaa tggcgtaatg atggccaggc tgtctccacc
     1081 cgagactcag tgaaattgaa ctcgctgtga agatgcagtg tacccgcggc aagacggaaa
     1141 gaccccgtga acctttacta tagcttgaca ctgaacattg agccttgatg tgtaggatag
     1201 gtgggaggct ttgaagtg
//
LOCUS       DAJTVK010000307         1214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000307 DAJTVK010000000
VERSION     DAJTVK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.313"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            231..>1214
                     /gene="gspA"
                     /locus_tag="OO372_004458"
     CDS             231..>1214
                     /gene="gspA"
                     /locus_tag="OO372_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCR6343744.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGN"
BASE COUNT          329 a          249 c          310 g          326 t
ORIGIN      
        1 agcatcttta ttatgattcg ccagatggaa ggggaaacgt agtgtgggca cgatgtatgt
       61 tctaataaaa tagattgggt gggttgtata ttgacgttag tataaatagt attaatgccg
      121 cttgcatgaa tcaagcaaaa tttatttata ttagtaatat gtatttaatt aataaataca
      181 tacattattt ttatgattaa ggataatcaa taattaaaac aggaagttct atgtctacga
      241 gaagagaagt tattctttcc tggttgcgtg agaaacgtca gacctggcgt ctgtgctatt
      301 tgttgggtga ggctggaagt ggaaaaacct ggctggcgca gcaactgcaa aaagataaac
      361 atcgccgtgt gattacttta agcctcgttg tttcctggca aggtaaggcc gcatggatcg
      421 ttaccgacga taacgcggct gaacagggct gccgtgacag cgcctggacg cgagatgaga
      481 tggcggggca attactgcat gcgcttcatc gaactgatag tcgctgccct cttattatta
      541 ttgaaaatgc tcacctgaat caccgccgca tacttgatga tctccaacgc gcgatatctc
      601 ttattcctga cggtcaattt ttgctgatcg gcagacccga tcgaaaagtc gaacgtgatt
      661 ttaaaaaaca ggacattgaa cttgtctcga caggacgtct gacggagcac gaacttaagg
      721 caagcatcct tgaaggtcag aatattgacc aacccgatct ccttttaacc tccagagttc
      781 tgaaacggat agctttatta tgtcggggcg atcgcagaaa gctggcgctt gccggcgaaa
      841 caattcgctt attacagcaa gccgagcaga ccagcgtttt cactgcaaaa caatggcgaa
      901 tgatttaccg catattgggt gataaccgcc cccggaaaat gcagcttgcc gtggtaatga
      961 gtggaaccat tatcgctatt acctgtggtt ggctactgct gtcctctttc acagctccgt
     1021 tccccgttcc tgcgtggctg gtacccgtaa cgccagtagt taaacaagat atgactaagg
     1081 atattgctca tgtggtgatg cgcgatagtg aggcgctaag cgtgttatac ggcgtatggg
     1141 ggtacgaggt cccagctgat tcggcctggt gcgatcaggc agtaagagcg gggctggcat
     1201 gtaaaagtgg gaat
//
LOCUS       DAJTVK010000308         1211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000308 DAJTVK010000000
VERSION     DAJTVK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.314"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..680)
                     /locus_tag="OO372_004459"
     CDS             complement(<1..680)
                     /locus_tag="OO372_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6343745.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1211)
                     /locus_tag="OO372_004460"
     CDS             complement(996..>1211)
                     /locus_tag="OO372_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HCR6343746.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          355 a          211 c          252 g          393 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aaatctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata ttctgataaa atgtgatact
      721 gggaaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagagatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat c
//
LOCUS       DAJTVK010000309         1189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000309 DAJTVK010000000
VERSION     DAJTVK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.315"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..294)
                     /locus_tag="OO372_004461"
     CDS             complement(<1..294)
                     /locus_tag="OO372_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6343747.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(342..914)
                     /locus_tag="OO372_004462"
     CDS             complement(342..914)
                     /locus_tag="OO372_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR6343748.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          379 a          246 c          220 g          344 t
ORIGIN      
        1 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
       61 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
      121 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
      181 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
      241 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
      301 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
      361 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
      421 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
      481 ttataaagtt taacctgcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
      541 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
      601 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
      661 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
      721 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
      781 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
      841 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
      901 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
      961 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gctttatcta
     1021 aaaataatat taattacgca aaaataaaat ataaattcat tgtttaaata ttatttaaac
     1081 agtaaaaaat caagtttgtt atcagacatt aatgataatt cagaatatat ttatatagaa
     1141 agatagataa atttattaat gtcagttata aaaaagggcc accggtcgg
//
LOCUS       DAJTVK010000310         1183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000310 DAJTVK010000000
VERSION     DAJTVK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.316"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..1002
                     /gene="htrE"
                     /locus_tag="OO372_004463"
     CDS             <1..1002
                     /gene="htrE"
                     /locus_tag="OO372_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCR6343749.1"
                     /translation="YWASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYL
                     SFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYT
                     MNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSND
                     SFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDV
                     ELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNV
                     GQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1019..1123
                     /locus_tag="OO372_004464"
                     /pseudo
     CDS             1019..1123
                     /locus_tag="OO372_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          359 a          236 c          276 g          312 t
ORIGIN      
        1 tactgggctt ccggacaaaa tcgtagcaat tactctattg gctacagtaa cagtgcatcc
       61 tggggcagct acagtgtcag tgcccagcgt tcatggaatg aagacggcga cactgacgat
      121 agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga acaacggact
      181 tcaggtttcc agagtattga tactcaaatg agcagtgact ttaagggtaa taaccaactc
      241 aacgtcagta gcagtggcta tagtgataac gctcgcgtca gttatagcgt aaatactggc
      301 tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc cagctatgaa
      361 tcaccgtggg gaaccctggc aggttcagtt tctgcaaata gcgataacag tcgtcaagtg
      421 tctctcagca ccgacggtgg ttttgtattg catagcggcg gcctgacctt cagtaatgac
      481 agttttagcg actccgatac actggcggta gttcaggctc caggtgctca aggagcgcga
      541 ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag cgctctttct
      601 ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga tgttgaatta
      661 aaaagtacca gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc tgattttgaa
      721 accgtgcaag ggcaatcagc cattatgaac atcacacgaa gtgatggtaa aaatattcca
      781 tttgctgcag atatttatga tgagcaaggc aatgtcattg gtaatgttgg acagggtgga
      841 caagcatttg ttcgtggcat tgagcagcag ggaaatatca gcattaaatg gctcgaagaa
      901 agtaaacccg taagttgtct tgcgcattat caacaaagtt cagaagcaga aaaaatagcg
      961 caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg aacgtcaaat
     1021 gataaaaata acgccacata aaatagcgat actgatggga atattattat caccatcagt
     1081 atttgcaacg gatattaatg tagagtttac agccactgtc aaagtagact ggccccctga
     1141 atctccagac aaccagtatc acttaaataa gtgatagtct taa
//
LOCUS       DAJTVK010000311         1148 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000311 DAJTVK010000000
VERSION     DAJTVK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.317"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            119..376
                     /locus_tag="OO372_004465"
     CDS             119..376
                     /locus_tag="OO372_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343750.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="OO372_004466"
     CDS             376..750
                     /locus_tag="OO372_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6343751.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="OO372_004467"
     CDS             747..>1148
                     /locus_tag="OO372_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6343752.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAJTVK010000312         1145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000312 DAJTVK010000000
VERSION     DAJTVK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.318"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..187
                     /locus_tag="OO372_004468"
     CDS             <1..187
                     /locus_tag="OO372_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6343753.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="OO372_004469"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="OO372_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6343754.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAJTVK010000313         1110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000313 DAJTVK010000000
VERSION     DAJTVK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.319"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            759..1055
                     /locus_tag="OO372_004470"
     CDS             759..1055
                     /locus_tag="OO372_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR6343755.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          330 a          213 c          236 g          331 t
ORIGIN      
        1 ttagctgtct cataatccaa aaacaacctt ttgagactac ttgagggtgc attctaccgt
       61 tacgttgaaa gtgtctcaaa aggaagtcct tttaagactg gttatgaagg agctatgagg
      121 ttgattaggg gttggtacaa gcccaactaa ccgaatcaac tatgaagatt ttaggagggt
      181 aactgtaacc atcactttct aactttcacg taggaaattg ttaagtggtt tgatagttac
      241 accatgtgac caagcatcta tatatcgtta tatagccctg tatatagcac tatatgtagt
      301 gtcgagagag gaacgactaa cacgatacag tttaccctga aaagagactt cacagattct
      361 ttatatattt caataagtta gggtaaaaag ggactatacc gagactgtat agaatctgtg
      421 tagaattgca tagatacttg gaaatctact attaaattgc atagggactc acctgatatt
      481 gtcctgtttt taacaggtct cctagaaaac cgaatagact tcattcaagg gtcattgaag
      541 tcataatcat ttcagtagaa aaaccaagtc tcgattttaa atgcaaatag caatcatatt
      601 caataaggca agacgatcag gatcagtgaa aatgtcattt ttggcgtaat tgaccttact
      661 ttatgcatgg tcttaacaaa gacatcatct ttccgttgca gatcttcgcc ctttcaaata
      721 acgtacttta caactttccc gaacaaggag ttgtgcccgt gttgtatgtt atttacgctc
      781 aagacaaagc tgatagcctc gaaaaacgcc tttccgttcg tccggcacat ttagcacgtt
      841 tacagttact gcatgatgaa ggtcgtttgt tgacagctgg tccaatgcca gcggtagaca
      901 gtaacgatcc aggggctgcg ggatttactg gctcaaccgt tattgctgaa tttgaatcgc
      961 tggaagcagc tcaggcctgg gccgatgctg acccgtacgt agcggcaggt gtctatgaac
     1021 acgtttcagt gaaaccattt aagaaagtgt tctgaataga gattcaatat ttaagggcac
     1081 ggtttttgca accgtgttct tgtttggcct
//
LOCUS       DAJTVK010000314         1088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.320, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000314 DAJTVK010000000
VERSION     DAJTVK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.320"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            212..>1088
                     /gene="nadA"
                     /locus_tag="OO372_004471"
     CDS             212..>1088
                     /gene="nadA"
                     /locus_tag="OO372_004471"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6343756.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT          294 a          259 c          265 g          270 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgca
//
LOCUS       DAJTVK010000315         1045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.321, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000315 DAJTVK010000000
VERSION     DAJTVK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.321"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            284..388
                     /locus_tag="OO372_004472"
                     /pseudo
     CDS             284..388
                     /locus_tag="OO372_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="OO372_004473"
     CDS             728..985
                     /locus_tag="OO372_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR6343757.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          179 c          198 g          311 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccc
//
LOCUS       DAJTVK010000316         1023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000316 DAJTVK010000000
VERSION     DAJTVK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.322"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(77..865)
                     /locus_tag="OO372_004474"
     CDS             complement(77..865)
                     /locus_tag="OO372_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6343758.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="OO372_004475"
     CDS             complement(905..>1023)
                     /locus_tag="OO372_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6343759.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAJTVK010000317         1019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000317 DAJTVK010000000
VERSION     DAJTVK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.323"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..1019
                     /locus_tag="OO372_004476"
                     /pseudo
     CDS             1..1019
                     /locus_tag="OO372_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          199 a          317 c          332 g          171 t
ORIGIN      
        1 ccggtgaaac cgacatcgcc ctgcccgccc cgctgccgtt catcctctcc cgcacctaca
       61 gcagttaccg gacaaaaacg cccgcgccgg tggggagcct cggccccggc tggaaaatgc
      121 ctgcggatat ccgcttacag ctgcgcgata acacactgat actcagtgat aacggcggca
      181 gaagcctgta ttttgagcac ctgtttcccg gtgaggacgg ttacagccgc agcgaatcac
      241 tctggctggt gcgcggcggc gtggcgaaac tggatgaagg tcaccggctg gccgcactct
      301 ggcaggcgct gccggaagaa ctccgcttaa gtccgcatcg ttatctggcg acaaacagtc
      361 cgcaggggcc gtggtggctg ctcggctggt gtgagcgggt gccggaagcg gatgaggtgc
      421 tgcctgcgcc gctgccgccg taccgggtac tgaccgggct ggtggaccgc ttcgggcgca
      481 cacagacgtt ccaccgcgaa gccgccggtg aattcagcgg cgaaatcacc ggcgtgacgg
      541 atggtgccgg gcgtcacttc cggctggtac tgaccacgca ggcgcagcgg gcagaagaag
      601 cccggcagca ggccatttcc ggcgggacgg aaccgtccgc ttttcctgat accctgccgg
      661 gttacaccga atatggccgg gacaacggca tctgtctgcc gtgtggctga cgcacgaccc
      721 ggaatacccg gagaatttac ctgccgcgcc gctggtgcgc tatggctgga cgccacgcgg
      781 cgaactggcg gtggtgtatg accgtagtgg caaacaggtg cgcagcttta cttacgatga
      841 taaataccgg ggccggatgg tggcgcaccg tcacacgggc cggccggaaa tccgttaccg
      901 ttacgacagc gacgggcggg tgacagaaca gcttaacccg gcaggcttaa gctacacgta
      961 tcagtatgag aaggaccgca tcaccatcac cgacagcctg aaccgccgtg aagtgctgc
//
LOCUS       DAJTVK010000318          963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000318 DAJTVK010000000
VERSION     DAJTVK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.324"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            70..963
                     /locus_tag="OO372_004477"
                     /pseudo
     CDS             70..963
                     /locus_tag="OO372_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          280 a          229 c          249 g          205 t
ORIGIN      
        1 aataatgagg tagcctgagt ttaacggaca ctccttcctg aaatagaatg gcatcagaag
       61 gagctaataa tgagcagaaa aacccaacgt tactctaaag agttcaaagc cgaagctgtc
      121 agaacggttc ttgaaaatca actttcgatc agtgaaggcg cttcccgatt atctcttcct
      181 gaaggcactt taggacaatg ggttaccgcc gccagaaaag ggctcggtac tcctggttcc
      241 cgcacggtgg ctgaactgga atctgaaatt ctgcaactgc gtaaggcgtt aaatgaagct
      301 cgccttgagc gagatatatt aaaaaaagca acagcgtatt ttgcacagga gtcgctgaaa
      361 aatacgcgtt aatcgaacaa tggcgacaac aatttcccat tgaagcgatg tgtcaggtat
      421 ttggtgtatc caggagcggt tattacaact gggtacagca tgaaccctca gacagaaaac
      481 aaagtgatga gcggctaaaa ctggagatta aggtggcaca tatccgcact cgcgaaacat
      541 atggaacccg gcggctccag acggagctgg cagagaatgg catcatcgtt ggtcgtgacc
      601 gactggcacg tcttcgtaag gagctaaggc tacgctgtaa gcagaaacgc aagttcagag
      661 cgactacgaa ctcgaaccac aatctgccag ttgcgccaaa tctgctgaac cagacgttcg
      721 ctcctacagc accaaatcag gtctgggtgg cggacctgac gtatgttgcc acacaggagg
      781 gatggttgta cctcgctggc atcaaagatg tttatacgtg cgaaattgtc ggctacgcca
      841 tgggagagcg catgacaaaa gagctgacag gtaaagccct gtttatggcg ctcaggagcc
      901 agcgcccacc tgccgggcta atccaccact ctgatcgagg ttcacagtac tgcgcatacg
      961 att
//
LOCUS       DAJTVK010000319          957 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000319 DAJTVK010000000
VERSION     DAJTVK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.325"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..395
                     /locus_tag="OO372_004478"
     CDS             <1..395
                     /locus_tag="OO372_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6343760.1"
                     /translation="INAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLAA
                     VLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVLD
                     GKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            569..>957
                     /locus_tag="OO372_004479"
     CDS             569..>957
                     /locus_tag="OO372_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343761.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          327 a          177 c          188 g          265 t
ORIGIN      
        1 tgattaatgc cattgtcaaa ccgttctatg aacgtcagat gagtaagtca gataatccaa
       61 ttgataaagt tgtaaggtca aaggtggaac ccacagacca agcattgact ggtgacatga
      121 actctttagc cgctgtttta tgggctatgg ttcagggcaa atcaaaagat gttccacagt
      181 tttcacctga agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa
      241 aggttatcaa accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg
      301 tacttgatgg aaaggttatt gatgaaagaa ccacatcgca tattaatcgt atgcaggaag
      361 ataccttgat tagctcagct tatccagagg aataaacaat gttagagcta ctaatattta
      421 ttgccacaca tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc
      481 gaatgctacg tgaaatcttc tgaacagata ccaacatcaa aaattagatt tcacgtagct
      541 cacgcttaac agaataggaa taacaacaat gtttgacaca aagcaaatta gatactcagg
      601 taaagatggt atctattttc accttagaga taatgtggaa tcttttcttg ccttaacagc
      661 tactgaacag atggactatg actcaccgat gatggtgaca actcaggcta tgcaatcggg
      721 gcaaactatc actgataatg ttcagagagc acctaagacc atctctatta gcggtgttgt
      781 agtggttgat tcgacgggtt cagttctatt aaccagacag gggcaacttg tagaaacctt
      841 tatttctacg gttgaacaat ggcgagacca gaagcaaatc atcactattg tttgtagaga
      901 tggaatagct atcactgata gtatcattac tcagtttaaa gcgagtaaag agcatac
//
LOCUS       DAJTVK010000320          934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000320 DAJTVK010000000
VERSION     DAJTVK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.326"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(195..>934)
                     /locus_tag="OO372_004480"
     CDS             complement(195..>934)
                     /locus_tag="OO372_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6343762.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          280 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga ccaa
//
LOCUS       DAJTVK010000321          918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000321 DAJTVK010000000
VERSION     DAJTVK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.327"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..221)
                     /locus_tag="OO372_004481"
                     /pseudo
     CDS             complement(1..221)
                     /locus_tag="OO372_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(361..>918)
                     /locus_tag="OO372_004482"
     CDS             complement(361..>918)
                     /locus_tag="OO372_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6343763.1"
                     /translation="AVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRL
                     HIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKF
                     ATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTNDKVFKAG
                     LRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          260 a          206 c          151 g          301 t
ORIGIN      
        1 atggtatcgt gaacaggaat accattttca aaatcaccat attgttttaa aaaatcgaga
       61 tgtgtttccc caaaatcctc tatatcttcc cagccttctg caccagaaat aacggcacaa
      121 atagtcaaca gtagaatatc cgataattta tgttccactt tccaggcttg tttttaatcg
      181 gggataatag aaatatgttc catcaatttt ttaagttcca ttttgttctc cttaattatg
      241 taaggagtat ttgatcatgt ataagcaata aaaaacagct tcaggtaata aggaatatct
      301 caatttttaa acataaaatg cgaattattt agtacaaaaa gtgaactcct cagggcaaga
      361 ttacgaaagt ccgctccccg caaggactga cgcgagatag tttctgtcca tggctgcttt
      421 tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa cattaatagc
      481 aatgtgccgt atccctgaaa ataattctgc tgcatttcct cttcttattt tgcagtcgtc
      541 ttcattcatt accacgtcca gacgccagtg cagattattc tccacgtgcc agtgatttcg
      601 aatcgctgtc gcaaacttct cagcggttaa atcagcagaa ctgatataat atctgaccat
      661 catttcgggc tctttctttt gctctgctat tattgagcga aaggagactg ccacgcataa
      721 ttttttcagc cctttccatt caaacgtgaa atcaataagt tcatcaggga catcgcaaac
      781 aatatgaaga cggatttctt ctctgccgtg actcttttca ctcattgcgg aactgtcatg
      841 cgctggatta tttaattctg tcagcggaaa ttttttctca aaggctttat ttagccgccc
      901 ctggtttcct tttaccgc
//
LOCUS       DAJTVK010000322          908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000322 DAJTVK010000000
VERSION     DAJTVK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.328"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>908
                     /gene="tuf"
                     /locus_tag="OO372_004483"
     CDS             <1..>908
                     /gene="tuf"
                     /locus_tag="OO372_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6343764.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGD
                     NIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          210 a          226 c          263 g          209 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      721 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      781 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      841 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      901 ggcggccg
//
LOCUS       DAJTVK010000323          873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000323 DAJTVK010000000
VERSION     DAJTVK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.329"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>873
                     /gene="cadA"
                     /locus_tag="OO372_004484"
     CDS             <1..>873
                     /gene="cadA"
                     /locus_tag="OO372_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCR6343765.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGM
                     EKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDF
                     KRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLP
                     TMVMTPYAAFQKEL"
BASE COUNT          236 a          219 c          212 g          206 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
      361 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
      421 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
      481 tggaaaaaga cggcaccatg agcaactttg gtattccggc cagcatcgtg gcgaaatacc
      541 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
      601 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
      661 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
      721 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
      781 ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg actatggtaa
      841 tgactccgta tgctgcgttc cagaaagagc tgc
//
LOCUS       DAJTVK010000324          849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000324 DAJTVK010000000
VERSION     DAJTVK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.330"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..761)
                     /locus_tag="OO372_004485"
                     /pseudo
     CDS             complement(1..761)
                     /locus_tag="OO372_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          199 a          204 c          221 g          225 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcaagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgcc
//
LOCUS       DAJTVK010000325          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000325 DAJTVK010000000
VERSION     DAJTVK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.331"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..516)
                     /locus_tag="OO372_004486"
     CDS             complement(<1..516)
                     /locus_tag="OO372_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6343766.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          267 a          173 c          152 g          233 t
ORIGIN      
        1 tgggttgctg gcatcattgc ctttcgcatc acccggacag ttagaagagg ctacccacca
       61 agcattttta tataccgcac cattaaagac gacataccag gtctgaccgc cctgctgttt
      121 attccaggca actacagaag aattagacgg aacagtgccc tgtgctggtg tagccgaacc
      181 gccattagcc ggcgtattgg atgcaggcgt atttgaattt gaagaagatg agctgccgct
      241 cttttcgcag gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt
      301 taaacgccac gggttagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaaa
      361 accccaccag gcattttcat aaattttgct atcgaaaata acctgatatt tattaccacc
      421 ttgttggtta ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat
      481 accaatcata gatttagtaa atatatttaa tttcattata atcccttgtg acgtaaaaac
      541 tgcaaaacaa aagtcctacg caggaattat ttacgtttta cgagaaccct tcaatatgaa
      601 ttaaagtgag gcattaaaaa taagaaaaaa gcctgacaaa tgaagaattt taaaaacaga
      661 aacattcata tttaaaatat taaattgaat tgatatttta aatatgaata atttattcgt
      721 tctgacagta cgaataagat atgcagtcaa caaatgcaaa aagggcgccg aagcgccctt
      781 ttcaattcaa aactaattaa cgtgtaatta gcccagaact ttagc
//
LOCUS       DAJTVK010000326          814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000326 DAJTVK010000000
VERSION     DAJTVK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.332"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(246..>814)
                     /locus_tag="OO372_004487"
     CDS             complement(246..>814)
                     /locus_tag="OO372_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6343767.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          258 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aaga
//
LOCUS       DAJTVK010000327          814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000327 DAJTVK010000000
VERSION     DAJTVK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.333"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            229..>814
                     /locus_tag="OO372_004488"
     CDS             229..>814
                     /locus_tag="OO372_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6343768.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          226 a          180 c          174 g          234 t
ORIGIN      
        1 cccaccggca gaagcagaaa aagcttatta tgcttccatc ggaaatgatg atctggcagc
       61 ctgagttcac agataaaaca ctctccagga aacccggggc ggttcagttc acaaaaacac
      121 attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca ttagcttctt
      181 cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt gaataaagtt
      241 tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga acttgcccgc
      301 agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg cattccagca
      361 atctgtctgt gttactctca gatatccctg gcgggtattg tccgttccga tattgcctat
      421 cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc caatgatatt
      481 ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc aatggccgat
      541 ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta tatcgtcagc
      601 gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa tacatattcc
      661 cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct gaataaactg
      721 gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa tgcttataaa
      781 tacactgaac gttacaccgc tttttatcgg gtgg
//
LOCUS       DAJTVK010000328          807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000328 DAJTVK010000000
VERSION     DAJTVK010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.334"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(1..411)
                     /locus_tag="OO372_004489"
                     /pseudo
     CDS             complement(1..411)
                     /locus_tag="OO372_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          199 a          221 c          182 g          205 t
ORIGIN      
        1 gtttcgcccc atcccgtaat gtttatcgat gctacggcgc gctgaggcgc tcaaatcgag
       61 ataatcagcc agttcatcga gggtataggc ttcctctcgc cactccatca cctgccattc
      121 caggtggaac ggatttttac aaatcagtcc gctgtagttc acatcagcac aaagtttctc
      181 acacagcgaa cgttcaatcg cagcggcgta tttcagcgcc ttaaccgagc tgtccggcgc
      241 ggttctcacg gcgatattga gcgcgtagag caggtgagca tgaccgttca caggattttt
      301 tacggtgatg ttcggcgcgg gagcgttccg gtcgctccag tcaatcgctg ccccctcgcg
      361 atcaacgtca aaaaccagcc agaactgcgc atgaggctgg ttctgccgga tctggacatg
      421 atttcagggg cgtttgtttt tcagtcgctt acactacacc caacggcaaa tcctgtcgaa
      481 ccttcccgaa aacccgatcc atttaccata actcccgttg tacgtgccct ctcagacgct
      541 ctctgacgca aaaaactcaa aaagacaaga aaaaacaggg taaacataaa aaatgcgtca
      601 gaatcgtttt tagcgcgttg cataactatg cacgaaagcg acccgcgatc ggttttgtgt
      661 gttctgaata ccgaacgggt caaaatgaca cgttttctct ccctcaatgg agcgttctat
      721 acagagattt ttgttcccct atccattatt ctgattaccg ggtttccaag ctgaaaacag
      781 gacttttctg cgaccagttt ttgcgaa
//
LOCUS       DAJTVK010000329          795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000329 DAJTVK010000000
VERSION     DAJTVK010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.335"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..741)
                     /locus_tag="OO372_004490"
     CDS             complement(<1..741)
                     /locus_tag="OO372_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6343769.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          161 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DAJTVK010000330          790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000330 DAJTVK010000000
VERSION     DAJTVK010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.336"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>790
                     /locus_tag="OO372_004491"
     CDS             <1..>790
                     /locus_tag="OO372_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCR6343770.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYINNIIKMIDTLPDNAMILKSVLAV
                     KLVMQLKILNIVNKNFIENMKKTFSHCPYIKDPIIRSYIHSGEDDKFDNFMRQHRFSK
                     VNFDTQQMIHFINRFNMNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRS
                     DDVSPIFIETEEKLRTFNTNKLTRNDNIFILFSSVDDGPVMVVSS"
BASE COUNT          281 a          115 c          134 g          260 t
ORIGIN      
        1 cgaatgtcag attagaaatt caaacttcga taattgttat ctttacgctt cgcacttcac
       61 cagagcagag tttctgtctg ccaaagaaat atcatttatt aaatcgaatt tgacagctgt
      121 tatgtttgat catgtgcgaa tgtcgacagg gaattttaaa gattgcatta cagaacaatt
      181 ggaattaact attgattatt cagatatatt tgggaatgaa gatctcgatg gttatatcaa
      241 taacattata aaaatgattg atacattgcc agataatgca atgatattga aatccgttct
      301 ggcagtaaaa ctggtgatgc aattaaaaat tctcaatatt gttaataaaa actttattga
      361 gaatatgaaa aaaacattta gccattgtcc ttatataaaa gatccaatta tacgtagtta
      421 tatacattct ggggaagatg acaagttcga taattttatg cgtcaacatc gattcagtaa
      481 ggtgaatttc gatacccaac agatgatcca ttttattaac agatttaata tgaataaagg
      541 gctgattgat aaaaataaca atttttttat ccaacttatc gatcaggcct tacgatcaac
      601 ggatgatatg atcaaagcaa atgcctggta tctttataaa gagtggattc gtagtgatga
      661 tgtttcacct atatttatag aaactgaaga aaagttaaga acctttaaca ctaataaatt
      721 aacacgaaac gataatatat ttatcctgtt ctcctctgtc gatgatgggc ctgttatggt
      781 ggtaagctcc
//
LOCUS       DAJTVK010000331          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000331 DAJTVK010000000
VERSION     DAJTVK010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.337"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            5..>747
                     /gene="ipaH3"
                     /locus_tag="OO372_004492"
     CDS             5..>747
                     /gene="ipaH3"
                     /locus_tag="OO372_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR6343771.1"
                     /translation="MLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGENRN
                     EAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASLEYL
                     DACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPTSLE
                     VLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENRITY
                     IPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          224 a          193 c          124 g          206 t
ORIGIN      
        1 catcatgtta ccgataaata ataacttttc attgtcccaa aattcttttt ataacactat
       61 ttccggtaca tatgctgatt acttttcagc atgggataaa tgggaaaaac aagcgctccc
      121 cggtgaaaat cggaatgaag cggtctccct acttaaagaa tgtctcatca atcagttcag
      181 tgagcttcaa ctgaatcgtt taaatctgtc ctcgctacct gacaacttac cacctcaaat
      241 cactgttctg gaaattactc agaatgccct aatatcatta ccagaattgc cagcatcgct
      301 ggaatacctt gacgcctgtg acaatcacct gtcaacactt cctgaattac ccgcatctct
      361 gaaacatctt gatgtagata acaaccaact aaccatgctt cctgaattgc ctgcattgct
      421 ggaatatatt aatgcagata acaatcagct aaccatgctt cctgaattac ctacatcgct
      481 ggaagtgctc tcagtaagaa ataaccagct gacatttctc cctgagttac ctgaatcact
      541 ggaagcgctc gatgtaagta ctaatcttct ggaaagccta ccagccgtac ctgtaagaaa
      601 tcatcactca gaggaaaccg agatattttt ccggtgccgc gagaatcgca tcacatacat
      661 tccggaaaat atacttagcc ttgatccgac ctgcactatc atcctcgaag acaatcctct
      721 gtcctcacgg atcagggagt ctctgtc
//
LOCUS       DAJTVK010000332          743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000332 DAJTVK010000000
VERSION     DAJTVK010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.338"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..396)
                     /locus_tag="OO372_004493"
     CDS             complement(<1..396)
                     /locus_tag="OO372_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6343772.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          172 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAJTVK010000333          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000333 DAJTVK010000000
VERSION     DAJTVK010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.339"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            168..686
                     /locus_tag="OO372_004494"
     CDS             168..686
                     /locus_tag="OO372_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6343773.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT          212 a          163 c          144 g          214 t
ORIGIN      
        1 acaatacgat taaattaatt aaaaccccac aaataaatta aacataagat tttactcatt
       61 gtgttttatt tctcacattg atgacgatcg catgaaaaaa tgatatttta atgagcattc
      121 tgaaaaaata tcgatgcatt tcgagcgaag atggtgggga tccctgaatg cgtttttctc
      181 accgactgtt cttacttctg attctcctgc tgacgggagc accaatactc gctcaggagc
      241 cttctgacgt tgcgaaaaac gtgcgtatga tggtttccgg tattgtcagt tacacccgct
      301 ggcctgcgct atcagggcca ccaaagttat gtatcttttc atcttcacgt tttagtaccg
      361 ccctacaaga gaatgccgcg acatctttgc cttatctgcc tgtcatcatt cacactcaac
      421 aagaggcgat gatttcaggc tgcaatggtt tttattttgg caacgagtca ccgacattcc
      481 agatggaatt aaccgaacaa tatccgtcaa aggcgttatt attaattgcc gaacaaaata
      541 ccgagtgcat tattggtagc gccttttgtc tgatcatcca caataatgac gtcagatttg
      601 ccgtaaacct ggatgcctta tcgcgtagcg gtgtaaaggt caacccggat gtcttaatgc
      661 tcgcacggaa gaaaaatgat ggataacgat aattctctta ataagcgccc cacgtttaaa
      721 agagcattac gca
//
LOCUS       DAJTVK010000334          710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000334 DAJTVK010000000
VERSION     DAJTVK010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.340"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAJTVK010000335          677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000335 DAJTVK010000000
VERSION     DAJTVK010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.341"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..361
                     /locus_tag="OO372_004495"
                     /pseudo
     CDS             1..361
                     /locus_tag="OO372_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAJTVK010000336          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000336 DAJTVK010000000
VERSION     DAJTVK010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.342"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..196
                     /locus_tag="OO372_004496"
     CDS             <1..196
                     /locus_tag="OO372_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6343774.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
     gene            complement(197..388)
                     /locus_tag="OO372_004497"
     CDS             complement(197..388)
                     /locus_tag="OO372_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6343775.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(493..>649)
                     /locus_tag="OO372_004498"
     CDS             complement(493..>649)
                     /locus_tag="OO372_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6343776.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT          182 a          147 c          162 g          158 t
ORIGIN      
        1 ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg
       61 gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt
      121 ggatattggc aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct
      181 tgtgcaattg caataattaa aaatcaccgt actcctgtgc aggtcgccag aaaccatcta
      241 taaaatcctc aatcggaaaa caaccgccat ggcggatccg ttgatcgctc atagaataaa
      301 gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt
      361 agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa
      421 gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc atcccgacgg
      481 gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt
      541 acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca
      601 ggcatcgagt tgcatacata aatcggggtc agatttacaa ggaatggtt
//
LOCUS       DAJTVK010000337          647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000337 DAJTVK010000000
VERSION     DAJTVK010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.343"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            4..>647
                     /locus_tag="OO372_004499"
     CDS             4..>647
                     /locus_tag="OO372_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6343777.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
BASE COUNT          223 a          104 c          132 g          188 t
ORIGIN      
        1 gtcatgagca aaatatcaga tttgaattat tctcaacaca ttacattagc cgacaatttt
       61 aaacaaaaaa gtgaagtttt aaatacctgg cgtgttggaa tgaataattt tgcccgtaat
      121 gccgaggggc aggataacac aagaaatatc cttgatccta agacattttt ggaatttttg
      181 gtaaaaatat ttactctagg ttatgtggat tttagcaaac gctccaacga agcgggaaga
      241 aatatgatgg ctcatattga gtcctcatct tatatcaaaa ataatgatgg cagtgagata
      301 atgaagtttg ttatgaataa tcctgaaggg gaacgagcgg atttatctaa ggtggaaata
      361 gaaatcacac tttccgcttt cactactatg gggactcgtc aaggacatac ggctattata
      421 tttcaacaac ctgatggttc gactaatcgt tatgaaggaa agtcctttga aagaaaagat
      481 gagagttcat tacacctgat tactaacaag attctggcat gttaccaacg cgaagctaac
      541 aaggaaatag ctcgtctatt aaatattccg caggagttaa ataattctca ggacttgaat
      601 aactcgcagg tgagttgtaa agattcagtt gattctacga ttacgga
//
LOCUS       DAJTVK010000338          641 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000338 DAJTVK010000000
VERSION     DAJTVK010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.344"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="OO372_004500"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="OO372_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6343778.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAJTVK010000339          632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000339 DAJTVK010000000
VERSION     DAJTVK010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.345"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>632)
                     /locus_tag="OO372_004501"
     CDS             complement(<1..>632)
                     /locus_tag="OO372_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343779.1"
                     /translation="PGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAAEGDV
                     AGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQV"
BASE COUNT          119 a          216 c          200 g           97 t
ORIGIN      
        1 acctgagaag aggaaacaga acggggactg gccggggaag ataaggaaga ggcgcgctgt
       61 ttacgggacg cttccgcccg ctgcgcctgc gtgctcagcg ccatgtgaaa acggcggccc
      121 gcgccgtccg tcacccccgt caccgcgccc gccacgtcac cctccgccgc ccggtggaaa
      181 gtcagtgagc gcccgaagcc atccaccacg accgtcagta cccggtatgc gggcggctcc
      241 ggccagctga gtatccacca cggcccctga aggctgtttg tcgccaggta cacatgcgga
      301 ctcaggcgaa catcttccgg cagcacctgc cagagcgcgc ttagcggctg cgaactgtgc
      361 tgcgccgcca ctccgccccg cgccagccag agtgactcgc tgcggctgta gcttatctcg
      421 ccgggaaaca gcggctcaaa gtgaatgctc ctgccgccgc tgtcgttgag tatcaggcct
      481 tcatcgcgta tctgtaagcg gatatcgaag ggcgctttcc agccgggacc aaacaccccc
      541 accggcgcgg gtgttctggt ccggtagctg ctgtaggcgc ggaaaagaat aaacggcagc
      601 gggcagggca gcgcaaggtc cgtctcgccc gg
//
LOCUS       DAJTVK010000340          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000340 DAJTVK010000000
VERSION     DAJTVK010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.346"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            450..>617
                     /locus_tag="OO372_004502"
     CDS             450..>617
                     /locus_tag="OO372_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HCR6343780.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          177 a          106 c          137 g          197 t
ORIGIN      
        1 tatgcaagaa cgtgccacgg ctggctgatg gatgttcgat agcgcgagtt tgaatgaaaa
       61 tcagccggag atgattttac ataattgcta cggaattatt caatacagga attgcttgcg
      121 tctgcatgga ttgacctgaa atattcccga aaatttctct aaaaaactcg aaaaaaatgg
      181 taactggttg aatgtattaa tatgcaatgg tacgtgtcag ggattaaaag atgaacgtaa
      241 atttatttaa cgcattaatt ttaaagggtt ttattgtttg ttgacgaaaa caggaatcgt
      301 gttcggtctc tttttatctg ttaaaagcca gaagcatttc cttcgctgac tttatagtca
      361 accataacac acactctact gtctgagtcc aacgtttttt aacattcttg ttaaaattat
      421 gtgatcttta gcgcgggagg aaaatattga tgaaacagcc tgcgcccgtt tatcagagaa
      481 ttgcgggtca tcaatggcga catatctggc tttctggcga tatacacggt tgtcttgagc
      541 agttgcgccg caaattatgg cattgtcgtt ttgatccgtg gcgagattta cttatctcag
      601 tgggagacgt tatcgat
//
LOCUS       DAJTVK010000341          609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000341 DAJTVK010000000
VERSION     DAJTVK010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.347"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(442..518)
                     /locus_tag="OO372_004503"
     tRNA            complement(442..518)
                     /locus_tag="OO372_004503"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:tct)
     gene            complement(532..608)
                     /locus_tag="OO372_004504"
     tRNA            complement(532..608)
                     /locus_tag="OO372_004504"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Arg,seq:tcg)
BASE COUNT          151 a          165 c          152 g          141 t
ORIGIN      
        1 tagcggtcat gtgatgccgg tttcccggta actcagcacc ggtatctgag tcaacgtttt
       61 ctctactggg tcatttccga tacgccctgc ctgctgacag gctttcatca catctgaaaa
      121 tatagcaccc tgactgatac tgtagtacct aaggttccag aaactgtgat gtatccggca
      181 cagaaaagcc cctccggaga gggctggaga gtggcgctat gtgccattgc atgatgccgg
      241 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      301 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      361 caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaaag gcccgcaaaa
      421 gcgagccagg gaaaataagt gtggcgcgtt gtactggatt cgaaccagtg accgattgct
      481 tagaaggcaa ttgctctgtc cggctgagct aacaacgcat gatgcagata atggaccgcc
      541 atcggggact tgaaccccgc gcagccagct tcgaaggctg gcgctctttc ctgatgagct
      601 aatggcggt
//
LOCUS       DAJTVK010000342          589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000342 DAJTVK010000000
VERSION     DAJTVK010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.348"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            289..>589
                     /gene="mcbR"
                     /locus_tag="OO372_004505"
     CDS             289..>589
                     /gene="mcbR"
                     /locus_tag="OO372_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6343781.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALA"
BASE COUNT          171 a          121 c          148 g          149 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctg
//
LOCUS       DAJTVK010000343          575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000343 DAJTVK010000000
VERSION     DAJTVK010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.349"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAJTVK010000344          565 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000344 DAJTVK010000000
VERSION     DAJTVK010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.350"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>565
                     /gene="feaR"
                     /locus_tag="OO372_004506"
     CDS             <1..>565
                     /gene="feaR"
                     /locus_tag="OO372_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCR6343782.1"
                     /translation="GYLTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITL
                     LDASRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQE
                     SMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILR
                     PEWIAGETGMSVRSLYRMFADKGLVVA"
BASE COUNT          142 a          132 c          148 g          143 t
ORIGIN      
        1 caggctacct cacctggcag gaagtaaaag gcagcgacga tgcctggttt tacaccgttt
       61 ttcagcttag tggtcaggca ataatggagc aggatgagcg tcaggtgcag attggcgctg
      121 gcgatattac gttactcgat gcctcacgcc cctgttcgct ttactggcag gagtcttcta
      181 aacagatttc attacttttg ccacgcactc tgctggaaca atattttccc catcaaaaac
      241 ctgtctgcgc agaaagactg gacgctgact tacccatggt gcaactcagt catcgcctgt
      301 tacaggagag catgaataat ccggcacttt ctgaaacaga aagtgaagct gcgctacagg
      361 cgatggtgtg tctgctgcgc ccggtacttc atcagcggga atctgttcaa cctcgccgtg
      421 aacgtcagtt tcaaaaagtg gttacgttga tagacgataa tattcgcgaa gagatattac
      481 gcccggagtg gatagccgga gagacaggta tgtcagtacg tagtttgtac cgaatgtttg
      541 ccgataaagg tttggtagtc gcgca
//
LOCUS       DAJTVK010000345          562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000345 DAJTVK010000000
VERSION     DAJTVK010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.351"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>562
                     /locus_tag="OO372_004507"
     CDS             <1..>562
                     /locus_tag="OO372_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6343783.1"
                     /translation="DSKPGTLNDFLIAPDEGDLKPDVVKRFEEMVAQAQQSAGAAAGN
                     AQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTANHLSEIAAAGEKAQQKSRDNLG
                     LKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGE
                     YSVAGPYDIIIPDTRFEGVLSIRWTDA"
BASE COUNT          137 a          148 c          173 g          104 t
ORIGIN      
        1 cgactcaaag cccggcacgc tgaatgactt tctgattgct cctgatgagg gcgacctgaa
       61 accggatgtc gtcaaacgct ttgaggaaat ggtggcgcag gcgcagcaga gcgccggggc
      121 cgcagccgga aacgcacagc agacggcgca ggatgtggcg gcagccgcag gttatgcccg
      181 cgcagcagag caggccaaaa atgacattga tgctgcgctg accggcactc tgaaaacggc
      241 taaccatctg tctgaaatcg cagcagcagg cgaaaaggca caacagaagt cccgggataa
      301 tctggggctg aaaagtgcgg ccacgatgga agcacagagc gacatttacg accggacaaa
      361 aggccgtctg gcgatacccg gcgcattcgg ctttgggtgt gcttttctgc ctgaagatgt
      421 tatccgtttt gacactaaga gtgatttcct ggcctgggta aggaatgcgc tgccaggtga
      481 atattccgtt gctggcccct acgacatcat catacccgac acacggtttg aaggggtgct
      541 cagcatccgg tggactgatg ca
//
LOCUS       DAJTVK010000346          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000346 DAJTVK010000000
VERSION     DAJTVK010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.352"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OO372_004508"
     CDS             <1..543
                     /gene="ipaH9.8"
                     /locus_tag="OO372_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6343784.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTDEVLALRLSENGSQLHHS"
BASE COUNT          141 a          136 c          166 g          114 t
ORIGIN      
        1 accggcgctc tgctctccct gggcagggaa atgttccgcc tcgaaattct ggaggacatt
       61 gcccgggata aagtcagaac tctccatttt gtggatgaga tagaagtcta cctggccttc
      121 cagaccatgc tcgcagagaa acttcagctc tccactgccg tgaaggaaat gcgtttctat
      181 ggcgtgtcgg gagtgacagc aaatgacctc cgcactgccg aagccatggt cagaagccgt
      241 gaagagaatg aatttacgga ctggttctcc ctctggggac catggcatgc tgtactgaag
      301 cgtacggaag ctgaccgctg ggcgctggca gaagagcaga aatatgagat gctggagaat
      361 gagtaccctc agagggtggc tgaccggctg aaagcatcag gtctgagcgg tgatgcggat
      421 gcggagaggg aagccggtgc acaggtgatg cgtgagactg aacagcagat ttaccgtcag
      481 ctgactgacg aggtactggc cctgcgattg tctgaaaacg gctcacaact gcaccattca
      541 taatcacatc gcataaa
//
LOCUS       DAJTVK010000347          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000347 DAJTVK010000000
VERSION     DAJTVK010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.353"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>557
                     /locus_tag="OO372_004509"
     CDS             <1..>557
                     /locus_tag="OO372_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6343785.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJTVK010000348          557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000348 DAJTVK010000000
VERSION     DAJTVK010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.354"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>557
                     /locus_tag="OO372_004510"
     CDS             <1..>557
                     /locus_tag="OO372_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6343786.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAJTVK010000349          550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000349 DAJTVK010000000
VERSION     DAJTVK010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.355"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..173)
                     /locus_tag="OO372_004511"
     CDS             complement(<1..173)
                     /locus_tag="OO372_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HCR6343787.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVL"
     gene            complement(431..>550)
                     /locus_tag="OO372_004512"
     CDS             complement(431..>550)
                     /locus_tag="OO372_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCR6343788.1"
                     /translation="AINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          135 a          159 c           93 g          163 t
ORIGIN      
        1 agcaccttcg cacccagcac cgggttcacc gggttagcat aggtaatccc tcccggacac
       61 accgaacagg ccactcccgt cggcgcacct atcagcacac ccgctgaacc ctgcacaata
      121 tccagtccct tgcgggtcat gtcgccctgc cgcgccgccg gttttccgct catggtttat
      181 tcctcttcat ctgaattgat ttacattagg ttggttattt tctcataccc acaactatat
      241 tttctctctg aatttattac gtgcaagaca atcgcagcac tctaactaac ttcgatgcaa
      301 tttgatgttc agaaaaatcc ctcattagat tattatcatg aaatacagct aatcatcttt
      361 agcttatata tttctcaatg tattcttttg aaaggatccg tgccctttca aactcttcag
      421 cagggcaaga ttacgaaagc ccgctccccg caaggactga cgccagatag tttctgtcca
      481 tggctgcttt tcgcatctta cgtcttaacc ctgccttgaa taccttatca ttcgtcaaaa
      541 tattaatagc
//
LOCUS       DAJTVK010000350          546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000350 DAJTVK010000000
VERSION     DAJTVK010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.356"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..546
                     /locus_tag="OO372_004513"
                     /pseudo
     CDS             1..546
                     /locus_tag="OO372_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAJTVK010000351          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000351 DAJTVK010000000
VERSION     DAJTVK010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.357"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..145
                     /locus_tag="OO372_004514"
     CDS             <1..145
                     /locus_tag="OO372_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6343789.1"
                     /translation="LRRDRPDIAVRYYYKGVLWSVGYFASSCGGAPISVIRQYIEQQQ
                     TPG"
BASE COUNT          182 a          122 c           89 g          151 t
ORIGIN      
        1 actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg ttttgtggag
       61 tgttggctat tttgccagta gctgcggagg tgcgccaata tccgtcatcc gccaatacat
      121 tgaacaacag caaacacctg gttaggtgga aaaccacgcc ttatatccct gccctgaagg
      181 acggggtttt acggcgcacc ggataagcct ggagattatc tacaccagca tggacatcat
      241 tgtactatgc cccgtacatg ccccatcaca tcctcaacat caaagtatta gagccttgca
      301 aaccattatg cgtctcagtt tgtctcatct atcttacgca aaaaaaaata gaactaatat
      361 caagcggcgc tatattgcgt attaaataaa accacctcaa ttacattaat aaatctaaac
      421 aaacatcatg caaataattt ccatggttat ttctaggcta tttaaattaa ataaataaat
      481 tatgagtcaa tcataaataa caacctttaa tataatttaa ctaccaaatt tatattcgtt
      541 caca
//
LOCUS       DAJTVK010000352          520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000352 DAJTVK010000000
VERSION     DAJTVK010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.358"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>520
                     /locus_tag="OO372_004515"
     CDS             <1..>520
                     /locus_tag="OO372_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6343790.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAJTVK010000353          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000353 DAJTVK010000000
VERSION     DAJTVK010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.359"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..188)
                     /locus_tag="OO372_004516"
     CDS             complement(<1..188)
                     /locus_tag="OO372_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6343791.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="OO372_004517"
                     /pseudo
     CDS             242..445
                     /locus_tag="OO372_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          135 c          118 g          129 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggatttc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaacg
//
LOCUS       DAJTVK010000354          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000354 DAJTVK010000000
VERSION     DAJTVK010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.360"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            30..206
                     /locus_tag="OO372_004518"
     CDS             30..206
                     /locus_tag="OO372_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343792.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          178 a           59 c          111 g          153 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaaagatc atcagaagtt cactttttgt actaaataat tcgcatttta tgtttaaaaa
      481 ttgagatatt ccttattacc t
//
LOCUS       DAJTVK010000355          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000355 DAJTVK010000000
VERSION     DAJTVK010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.361"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            1..480
                     /locus_tag="OO372_004519"
                     /pseudo
     CDS             1..480
                     /locus_tag="OO372_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          112 a          116 c          142 g          110 t
ORIGIN      
        1 ccagtgcacg cattatgggc gttggcctca acacggtttt acgtcactta aaaaactcag
       61 gccgcagtcg gtaacctcgc gcatacaacc gggcagtgat gtgattgtct gcgctgaaat
      121 ggacgaacag tggggctacg tcggtgctaa atcacgtcag cgctggctgt tttacgcgta
      181 tgacaggata cggaggacgg ttgtggcgca cgtcttcggt gaacgcactc tggccacact
      241 ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatagatga cggatggctg
      301 gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt atcagcaagc gttacactca
      361 gcgcattgag cgacataatc tgaatctgag acaacatctg gcaaggctgg gacggaagtc
      421 actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat
//
LOCUS       DAJTVK010000356          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000356 DAJTVK010000000
VERSION     DAJTVK010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.362"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>476
                     /locus_tag="OO372_004520"
     rRNA            <1..>476
                     /locus_tag="OO372_004520"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAJTVK010000357          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000357 DAJTVK010000000
VERSION     DAJTVK010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.363"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>473)
                     /locus_tag="OO372_004521"
     CDS             complement(<1..>473)
                     /locus_tag="OO372_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6343793.1"
                     /translation="VYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGMS
                     WAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPIA
                     QELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLPFYAS"
BASE COUNT          162 a          107 c           98 g          106 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag aca
//
LOCUS       DAJTVK010000358          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000358 DAJTVK010000000
VERSION     DAJTVK010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.364"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            89..451
                     /locus_tag="OO372_004522"
     CDS             89..451
                     /locus_tag="OO372_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6343794.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAJTVK010000359          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000359 DAJTVK010000000
VERSION     DAJTVK010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.365"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(97..>467)
                     /locus_tag="OO372_004523"
     CDS             complement(97..>467)
                     /locus_tag="OO372_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR6343795.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          139 a          124 c           82 g          122 t
ORIGIN      
        1 ataaagccgc tgccgcagaa ttagcgctcg gttaagctga agaaataagg ccggatgtat
       61 gaattacgct ccggcctgtt ctcattattt aaataactac gaaaaatcac cccacagtct
      121 cagcttgccg cagtggagac agcggaagag ataaccctga caatcaccac ccttttgcag
      181 gcatttagct agatcagaat ttctaatacc gaaattctca caatcttctt caaggttggc
      241 aaattcatcg aggcgatctt ttatatcatt ccagcccaca tagccgataa aagcacagaa
      301 atcgccacaa tgcgcaagcc agaattcctg ttgccatcca tgataacctg gcgtgcgttc
      361 aaccaactct ttcagcattt catcaggata tgtgttctta ataccggcaa attcgtcctc
      421 ttcatcatac tcaaattcaa caccttctat gctggcatca tcctgaa
//
LOCUS       DAJTVK010000360          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000360 DAJTVK010000000
VERSION     DAJTVK010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.366"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>464
                     /locus_tag="OO372_004524"
     CDS             <1..>464
                     /locus_tag="OO372_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HCR6343796.1"
                     /translation="QHDFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQ
                     RWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT          112 a          115 c          137 g          100 t
ORIGIN      
        1 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
      121 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
      181 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
      241 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
      301 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
      361 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa agtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAJTVK010000361          454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000361 DAJTVK010000000
VERSION     DAJTVK010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.367"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..386
                     /locus_tag="OO372_004525"
     CDS             <1..386
                     /locus_tag="OO372_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6343797.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           95 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agca
//
LOCUS       DAJTVK010000362          452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000362 DAJTVK010000000
VERSION     DAJTVK010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.368"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>452)
                     /locus_tag="OO372_004526"
     CDS             complement(<1..>452)
                     /locus_tag="OO372_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6343798.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPL"
BASE COUNT           75 a          145 c          128 g          104 t
ORIGIN      
        1 caacggctgt ggccgccatg ttctgtctgc cgggattacc gcaaaatggt tctccagcgt
       61 ggtgcctgaa cccctgcgtc ccggtaccgc ctgcggctgt tcgccgtgaa gatcgctcgc
      121 caccacctgc cattcccggt tcaggttcgc ctgcggatgc cccgtcagca caattcgccg
      181 tcccggccat atctccggcg aacggcttgt tccgcgcgcc acttctgcgt tgtttcgcca
      241 gccgtccatc tgccagcggg caaagttctg cccgtgggca cccttgaaac gtccggggta
      301 gtcatacact tcatactgcg tgcgctggta atcctggtac tggccttcct gatcaaaacg
      361 ccctggccag ccgggacgtt taaaggtgta gtctttggtc actacggaag aaggacggat
      421 ttgtgcgctg tagcgaaact ggctgatgca ga
//
LOCUS       DAJTVK010000363          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000363 DAJTVK010000000
VERSION     DAJTVK010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.369"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>444
                     /locus_tag="OO372_004527"
     rRNA            <1..>444
                     /locus_tag="OO372_004527"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAJTVK010000364          427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000364 DAJTVK010000000
VERSION     DAJTVK010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.370"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..96
                     /locus_tag="OO372_004528"
     CDS             <1..96
                     /locus_tag="OO372_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6343799.1"
                     /translation="NIQHSTFNIQQGKKLVTGMDVNIDDEIGDDE"
     gene            96..>427
                     /gene="yhaV"
                     /locus_tag="OO372_004529"
     CDS             96..>427
                     /gene="yhaV"
                     /locus_tag="OO372_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCR6343800.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          136 a           81 c          100 g          110 t
ORIGIN      
        1 aacattcaac attcaacatt caacattcaa caaggaaaga aacttgtcac tggcatggac
       61 gtcaacattg atgatgagat tggcgacgac gaataatgga ttttccacaa agggttaatg
      121 gttgggcgct atatgctcat ccctgttttc aggaaaccta cgacgcttta gttgccgaag
      181 tcgagacatt aaagggaaaa gatcctgaaa attatcagag aaaagccgcc acaaagttat
      241 tggcggtagt ccataaagtg attgaggagc atatcacggt caatccatca tcaccggcat
      301 tccgtcatgg caagtcgtta ggctctggga aaaataaaga ctggtcacgg gtaaaatttg
      361 gtgcaggtcg ttatcgtctc ttctttcgtt atagtgaaaa agagaaagtc atcattctgg
      421 gatggat
//
LOCUS       DAJTVK010000365          427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000365 DAJTVK010000000
VERSION     DAJTVK010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.371"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>427
                     /locus_tag="OO372_004530"
     rRNA            <1..>427
                     /locus_tag="OO372_004530"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           93 a           92 c          144 g           98 t
ORIGIN      
        1 tgtttgatgt tctaacgtgg acccgtgatc cgggttgcgg acagtgtctg gtgggtagtt
       61 tgactggggc ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg
      121 tcggacatca ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc
      181 gagcaggtgc gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc
      241 aacggataaa aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg
      301 gcggtgtttg gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg
      361 gtatggctgt tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt
      421 tcggtcc
//
LOCUS       DAJTVK010000366          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000366 DAJTVK010000000
VERSION     DAJTVK010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.372"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            41..116
                     /locus_tag="OO372_004531"
     tRNA            41..116
                     /locus_tag="OO372_004531"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAJTVK010000367          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000367 DAJTVK010000000
VERSION     DAJTVK010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.374"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            54..130
                     /locus_tag="OO372_004532"
     tRNA            54..130
                     /locus_tag="OO372_004532"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="OO372_004533"
     tRNA            173..248
                     /locus_tag="OO372_004533"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAJTVK010000368          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000368 DAJTVK010000000
VERSION     DAJTVK010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.375"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            14..121
                     /locus_tag="OO372_004534"
     CDS             14..121
                     /locus_tag="OO372_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6343801.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           96 a           92 c          131 g           86 t
ORIGIN      
        1 tacaggcggc tatatgacgc tcgcagaact gggcatggcc ttctggcatg atttagcggc
       61 tccggtcatt gctggtattc ttgccagtat gatcgtgaac tggctgaaca agcggaagta
      121 acgtgccatg cgggcgtcag gctgccgtaa tggcaatttg cgcccggatc aggccgcagg
      181 ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg gttgcaagcg gataatattc
      241 tgcggaatgc gcagcacaga gtgaagacgt gacggattcc ggaacagaaa aacagtcgta
      301 gaatcagcgt caagccggaa gggttccggt gaggcgcaat gttgcggggg ctttatccct
      361 ggtggcattg gttgctggag agagaaaacc cccgcacgtt gcagg
//
LOCUS       DAJTVK010000369          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.376, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000369 DAJTVK010000000
VERSION     DAJTVK010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.376"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..356)
                     /locus_tag="OO372_004535"
     CDS             complement(<1..356)
                     /locus_tag="OO372_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR6343802.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          105 a          101 c          118 g           77 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggtcaact g
//
LOCUS       DAJTVK010000370          398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000370 DAJTVK010000000
VERSION     DAJTVK010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.377"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..363)
                     /locus_tag="OO372_004536"
     CDS             complement(<1..363)
                     /locus_tag="OO372_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105657.1"
                     /note="phosphorylates aminoimidazole ribotide (AIR) which
                     forms 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate
                     (HMP-P); synthesis of HMP-P from AIR is complex and
                     requires the product of thiC gene at least; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCR6343803.1"
                     /translation="MPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDDPFGAL
                     MQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETTDLPCW
                     RHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           84 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatactt
//
LOCUS       DAJTVK010000371          397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000371 DAJTVK010000000
VERSION     DAJTVK010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.378"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>397
                     /locus_tag="OO372_004537"
     CDS             <1..>397
                     /locus_tag="OO372_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HCR6343804.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          104 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatg
//
LOCUS       DAJTVK010000372          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000372 DAJTVK010000000
VERSION     DAJTVK010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.379"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            251..>391
                     /locus_tag="OO372_004538"
     CDS             251..>391
                     /locus_tag="OO372_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6343805.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSL"
BASE COUNT          121 a           69 c           66 g          135 t
ORIGIN      
        1 ctaaacttaa taaaacctta agattaacat tttaatataa cgagttgcat ttagttacat
       61 gtttaacact tgatttaaga tttgtaatgg ctagattgaa atcagatgta atccattagt
      121 tttatatttt acccatttag ggctgattta ttactacaca cagcagtgca acatctgtca
      181 gtacttctgg tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg
      241 gggtttcatc atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac
      301 taaaccttct ttgcaggcga cggctctctt gcagcacctt aaacaatcgc tggcaattac
      361 aggaaaatta cataatattc aacgttctct g
//
LOCUS       DAJTVK010000373          390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000373 DAJTVK010000000
VERSION     DAJTVK010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.380"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>390)
                     /locus_tag="OO372_004539"
     CDS             complement(<1..>390)
                     /locus_tag="OO372_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6343806.1"
                     /translation="IYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPHFLEHLGNNHL
                     DIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALH
                     GQFDSISMFYLLHCLPGNISTKSCVIR"
BASE COUNT          120 a           78 c           82 g          110 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt
//
LOCUS       DAJTVK010000374          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000374 DAJTVK010000000
VERSION     DAJTVK010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.381"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(70..>389)
                     /gene="tabA"
                     /locus_tag="OO372_004540"
     CDS             complement(70..>389)
                     /gene="tabA"
                     /locus_tag="OO372_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6343807.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          105 a          105 c           81 g           98 t
ORIGIN      
        1 aaaaaatatc aaaaaacgct cttttcacaa actcgccgta aacctggagt tatttacggc
       61 gaaaagtcat cacgccatca gcatcttcac tactgctttg cgaacctggg ctggtgcacc
      121 cactgcgcac agcggtttat gcacttcccc cggataaaac acaacaaaat caccttcatt
      181 aaggataact gttttctcat caacgccttc cggcaaaaat gcgatgtctt tatcagctaa
      241 ccagtcggta tccggcgtgc ctgcaggttg cgtgctgaag gtcatgcctt cctgaccttt
      301 taacacaatc tgaatgtcga gatagcgggc atggtactcc gtacggcgag cttcgtgcgg
      361 ctcggtcata tcttccgaga taagataaa
//
LOCUS       DAJTVK010000375          386 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000375 DAJTVK010000000
VERSION     DAJTVK010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.383"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..>386)
                     /locus_tag="OO372_004541"
     CDS             complement(<1..>386)
                     /locus_tag="OO372_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR6343808.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRA"
BASE COUNT          104 a          107 c           80 g           95 t
ORIGIN      
        1 tgcgcgggat ttagcgtaag ccgtttgtgt cgtctggtca gtttggagaa tgttgccatg
       61 caccgtacgc cagacagtaa aggtctctgc ctgaccattt ttcaccgtaa tggtttcctc
      121 acggcttaac attttcaccc acttaccatt atgcaagtag tagcctggtt tctctgccaa
      181 cagccgttca gcaaaaatat cgacatcatc gccgaaacct gccgttgatc cccaggaaat
      241 cacaccatta tgaccaaaaa ccagcccagg ataggcaaat ggtgtattgc cagtgacatc
      301 ataaccagca ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg
      361 cggaccattt accatgattg ctttcg
//
LOCUS       DAJTVK010000376          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000376 DAJTVK010000000
VERSION     DAJTVK010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.384"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            85..>365
                     /locus_tag="OO372_004542"
     CDS             85..>365
                     /locus_tag="OO372_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6343809.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          114 a           75 c           84 g           92 t
ORIGIN      
        1 atataataac tggatcacca aagataatgg cgagtttgaa ctggcggtcc attaattatc
       61 attgtaataa acgaaggaat agtaatgaaa aaggtatttg caaaatctct tctggtcgca
      121 gcgatgtttt ctgttgcagg ctccgcattg gcagtacaaa aggatattac tgtaacggcc
      181 aacgttgatg ccgctctgga tatgacacag accgataaca ccgcgttgcc gaaagcagta
      241 gaaatgcaat atctgccggg gcagggtctg caatcttacc agttgatgac caaaatctgg
      301 tctaacgacg ttaccaaaga tgtaaaaatg cagttagttt ccccggcgca actggttcaa
      361 agcct
//
LOCUS       DAJTVK010000377          338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.386, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000377 DAJTVK010000000
VERSION     DAJTVK010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.386"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..263)
                     /locus_tag="OO372_004543"
     CDS             complement(<1..263)
                     /locus_tag="OO372_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6343810.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT           88 a           84 c           66 g          100 t
ORIGIN      
        1 gtgtctggtt cttccggcca ctcaatatca ggtgcagttg atgtatcaac acggttcagc
       61 aacacccgat acttcttcca ggcttccagc aacgagtttt cttcctccgt tgcgatctcc
      121 agatctacag catcctgaag tggcgcaata tgctcactga attcctggat gtagaactgt
      181 gtggtgacgg tcttccagcc attcggctcc tgctgtatcg aagcatacca ggctatttca
      241 atatcgctat gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat
      301 tcacaaataa aactataata tggtgaaatt aatgaaaa
//
LOCUS       DAJTVK010000378          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000378 DAJTVK010000000
VERSION     DAJTVK010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.387"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            71..146
                     /locus_tag="OO372_004544"
     tRNA            71..146
                     /locus_tag="OO372_004544"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:105..107,aa:Glu,seq:ttc)
BASE COUNT           86 a           72 c           75 g           83 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt cttgcgaagc
       61 agactgatac gtccccttcg tctagaggcc caggacaccg ccctttcacg gcggtaacag
      121 gggttcgaat cccctagggg acgccacttg ctggtttgtg agtgaaagtc acctgcctta
      181 atatctcaaa actcatcttc gggtgatgtt tgagatattt gctctttaaa aatctggatc
      241 aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt gagtctctcg aattttcgca
      301 acacgatgat gaatcg
//
LOCUS       DAJTVK010000379          308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000379 DAJTVK010000000
VERSION     DAJTVK010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.denovo.388"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..>308
                     /locus_tag="OO372_004545"
     CDS             <1..>308
                     /locus_tag="OO372_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6343811.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAJTVK010000380         9600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.guided.144, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000380 DAJTVK010000000
VERSION     DAJTVK010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.guided.144"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(16..204)
                     /locus_tag="OO372_004546"
     CDS             complement(16..204)
                     /locus_tag="OO372_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6343812.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMLPTY"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OO372_004547"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="OO372_004547"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6343813.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="OO372_004548"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="OO372_004548"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6343814.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="OO372_004549"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="OO372_004549"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6343815.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="OO372_004550"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="OO372_004550"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6343816.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="OO372_004551"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="OO372_004551"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6343817.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="OO372_004552"
     CDS             5099..6232
                     /locus_tag="OO372_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR6343818.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="OO372_004553"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="OO372_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6343819.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="OO372_004554"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="OO372_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6343820.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="OO372_004555"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="OO372_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6343821.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="OO372_004556"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="OO372_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6343822.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="OO372_004557"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="OO372_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6343823.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="OO372_004558"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="OO372_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6343824.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2438 a         2466 c         2463 g         2233 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaat
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggt ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccatccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
//
LOCUS       DAJTVK010000381         9366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.guided.151, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000381 DAJTVK010000000
VERSION     DAJTVK010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.guided.151"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            complement(<1..1454)
                     /gene="pepD"
                     /locus_tag="OO372_004559"
     CDS             complement(<1..1454)
                     /gene="pepD"
                     /locus_tag="OO372_004559"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6343825.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPA"
     gene            1715..2173
                     /gene="gpt"
                     /locus_tag="OO372_004560"
     CDS             1715..2173
                     /gene="gpt"
                     /locus_tag="OO372_004560"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6343826.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2265..3509
                     /gene="frsA"
                     /locus_tag="OO372_004561"
     CDS             2265..3509
                     /gene="frsA"
                     /locus_tag="OO372_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6343827.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3567..3968
                     /gene="crl"
                     /locus_tag="OO372_004562"
     CDS             3567..3968
                     /gene="crl"
                     /locus_tag="OO372_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6343828.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(4007..5056)
                     /gene="phoE"
                     /locus_tag="OO372_004563"
     CDS             complement(4007..5056)
                     /gene="phoE"
                     /locus_tag="OO372_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6343829.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVMHYMSD
                     NASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKY
                     KDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGV
                     IDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQ
                     SRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDF
                     GLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLN
                     INNDDIVAVGMTYQF"
     gene            5344..6447
                     /gene="proB"
                     /locus_tag="OO372_004564"
     CDS             5344..6447
                     /gene="proB"
                     /locus_tag="OO372_004564"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6343830.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            6459..7712
                     /gene="proA"
                     /locus_tag="OO372_004565"
     CDS             6459..7712
                     /gene="proA"
                     /locus_tag="OO372_004565"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6343831.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            7827..7902
                     /locus_tag="OO372_004566"
     tRNA            7827..7902
                     /locus_tag="OO372_004566"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7860..7862,aa:Thr,seq:cgt)
     gene            8508..8666
                     /locus_tag="OO372_004567"
     CDS             8508..8666
                     /locus_tag="OO372_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343832.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2371 a         2246 c         2343 g         2406 t
ORIGIN      
        1 ttcgccggaa tttctttcag cagttcagtc agcagtgtcc agtaatgacc tacgctttcg
       61 atgtgaactt gctcatccgg agagtgtgga ccggtgatag ttggcccgat agaaaccatg
      121 tccatttccg gatacggttt tttgaacaga ccacattcca ggcccgcgtg gataatctgg
      181 atgttcggcg ttttgttgaa cagacgctga taggtttcac gtaccagatg catcaccgga
      241 gaattagcgt ccggctgcca gccaggatat gcgcctttcg cttcggtttt cgcgccagcc
      301 agtttaccca gcgaatccag catgctcacc acgtagtctt taccgctgtc gatcagagaa
      361 cggatcagac agtgaatttc tacgttatta tcagtcatgg tcaccacacc gacgttcagg
      421 gaggtttcga ccacaccttt tgccacgtct gagttgcgga tcacaccgtt cggggtggcg
      481 ttcagcagac gaataaaggt atcgcgagat ttcgcaatca gggcagcttt atcgttcgct
      541 acagagtcca gcaacaaggc cagatttttc tctttctctg ccagctcgtt tttcaggatc
      601 tcctgatagg tattcaccag agatttcagg acgtcgactt tatcagctgc gacagcaatg
      661 gtcgcaaagg cttcacgcgg gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg
      721 cgcagatcca gttcttctgc atgacccgct aggaagcgca ccagcagttt gttggcatta
      781 cccaggccaa cgtggatttc cccgccggag tgaccccctt tcagaccttt taaggttaac
      841 ttgaaggttt caaaaccagc tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag
      901 tcgatacccc ccgcacaacc catgtagatt tcaccttctt cttcggagtc ggtgttaatc
      961 agaatatcag cctgcaacca gttgctctgt aagccgaacg caccgtccat accggcttct
     1021 tcggtcatgg tcagcagcac ttccagcggg ccgtgaacca cgttttcgtc agccagaacc
     1081 gccagcgcag acgccatacc aatgccgtta tctgcaccca gcgtggtgcc gcgcgcttta
     1141 acccattcgc catcaatata aggctggata ggatctttcg tgaagtcatg cacggtgtcg
     1201 ttatttttct gcggcaccat atcgaggtgg gcctgtaaga cgaccgattt acgattttcc
     1261 atacctgcgg tagcaggttt acgaatcagg atattaccta cctgatcgcg ttcgacatgg
     1321 aaacctttct cttttgccca accaacaatg tattcagcga gttgctcttc atgataggac
     1381 gggtgaggaa tagaacagat tttggcaaaa atatcccaca gcggctgtgg agataattga
     1441 gacagttcag acacgttaag tctccttgtc gatcacccgc aaaacagtat tgcaggtcac
     1501 agggttagca gaaaatgttg tcaacacaag acaggcttgc gagatatgtt tgagaatacc
     1561 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     1621 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     1681 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     1741 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     1801 gaaaggcatt attgccgtaa gccgtggcgg tctggtaccg ggtgcgttac tggcgcgtga
     1861 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     1921 cgagcttaaa gtgctaaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     1981 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     2041 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     2101 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     2161 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     2221 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     2281 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     2341 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     2401 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgtgaaa
     2461 tcctcgacgt ccaggcgcgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     2521 acgatacggt gattggctac cgtggtggca actggattta tgagtgggcc atccaggcga
     2581 tggtgtggca acaaaaagcc tgtgcggaag aagatccgca actcagtggt cgtcactggc
     2641 tgcatgcggc tacgttgtac aacattgctg cctatcctca tctgaaagga gatgacctgg
     2701 ccgagcaagc gcaggctttg tcaaaccgtg cctatgaaga agccgcccag cgtctgccgg
     2761 gcacgatgcg gcagatggag tttaccgtac ccggcggtgc gcccatcacc ggctttttgc
     2821 atatgccgaa aggcgatggc ccgttcccga cagtattaat gtgtggtggt ctggatgcga
     2881 tgcagacgga ctattacagc ctgtatgaac gttattttgc gccgcgcggc attgcgatgc
     2941 tgactattga tatgccgtcg gtgggctttt catcaaaatg gaagctcacc caggactcca
     3001 gcctgttgca tcagcacgtt ttaaaggctc tgcctaacgt accgtgggtg gatcacactc
     3061 gcgtcgcggc ctttggtttc cgtttcggcg ctaacgttgc cgtgcgtctg gcatatcttg
     3121 aatcgccgcg tctgaaagcg gttgcctgtc ttggtccggt agttcatacc ctgttgagtg
     3181 attttaagtg ccagcaacag gtgccggaaa tgtatcttga cgttctggcg agtcgtttgg
     3241 ggatgcatga tgcttccgac gaagcgttac gcgtggagct gaatcgctat tcattaaaag
     3301 tgcaaggatt gctggggcgt cgctgcccaa cgccaatgtt atcaggctac tggaagaacg
     3361 atccgttcag cccggaagag gactcacgct taatcacctc atcatctgct gacggtaaat
     3421 tattagagat cccatttaac ccggtgtatc ggaattttga caaaggtctt caggaaatca
     3481 ccggctggat cgaaaaacgc ttgtgttaaa aatttgctaa aatttgccaa tttggtaaaa
     3541 cagttgcatc acaacaggag attgcaatga cgttaccgag tggacacccg aagagcagat
     3601 tgatcaaaaa atttaccgca ctaggcccgt atattcgtga aggtaagtgc gaagataatc
     3661 gattcttttt cgattgtctg gctgtatgcg tcaacgtgaa accggcaccg gaagtgcgtg
     3721 agttctgggg ctggtggatg gagcttgaag cgcaggaatc ccgttttacc tacagttacc
     3781 agtttggtct gttcgataaa gcaggcgact ggaagagtgt tccggtaaaa gacactgaag
     3841 tggttgaacg actggagcac accctacgtg agtttcacga gaagctgcgt gaactgctga
     3901 cgacgctgaa tctgaagctg gaaccggcgg atgattttcg tgatgaaccg gtgaagttaa
     3961 cggcgtgagt gaaatgtgcc ggatgtatca catccggcaa tatccattaa aactgatagg
     4021 tcatgccaac cgcgacaata tcatcattat taatattcaa tttgttatcg ctatccagtt
     4081 ggttgatttt ataatcaaca aacgctgaca tatttttgtt gaaataatac gtagcaccga
     4141 cgtcgatata attgaccaga tcttcatcac cgataccttc aatatctttc cctttcgata
     4201 agacataacc cagcgatgga cgcagaccaa agtcaaactg gtattgagcg accgcttcaa
     4261 agttctgtgt cttattggca aagccgccag ttattggcgt cattttgcgt gtttcagaat
     4321 agaaagttgc cagataaata ttattggcat cgtatttcag acctgttgcc catgcttctg
     4381 cacgcttgcc tgtgccacgg ctttgcaggt tctgctcgtt ggtgcgatct gagttggtat
     4441 aggccccact aatggcgaaa tcgctgccgc caaagtcata tgtcaatgac gtgccgaagc
     4501 catcgccgtt ttgcttttta acgtcgcggt tttcgttttt cccttgatat tgcagggtta
     4561 agttcaggcc atcgataacg ccgaagaagt cggtgttccg atacgtcgcc agaccgctgg
     4621 cgcgtttggt cataaagttg tcggtctgcg ccgaggaatc gccaccaaat tccgggaaca
     4681 tatcggtcca ggcttccacg tcatacaacg cgcccaggtt acgaccataa tcgaaagaac
     4741 ccaaatcttt atatttcaac ccggcaaaag cgagacgcgt tttctgctgt gcagtatcac
     4801 tctccgcttt atttccggca aactccgctt cccaccgacc ataaccagtc agttgatcgt
     4861 taatttgtgt ttcaccttta aaaccaaaac ggatataact ctggtcgcca tctttactgg
     4921 cgttatcact catataatgc ataactttgc catagacatc cagtttatta ccgtctttat
     4981 tatatatttc tgcagcctgt acagatgcag atgccacaat gcccatcacc actaatgcca
     5041 gagtgctctt tttcattttc attcctgatt ttaattaacg cgcgaatatt cagcgggaga
     5101 gccccgttga aaacaggaaa gtttttaacc tgagattgtt aaagatatat tacagattaa
     5161 taatattctt aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc
     5221 taggcttgct gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg
     5281 acgacagtcc tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga
     5341 atcatgagtg acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga
     5401 tcgcgccgtc tgaaccgtgc ccatattgtt gaacttgttc gccagtgcgc gcagttacat
     5461 gccgccgggc atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac
     5521 ctgggttacc cggaactgcc agcgactatc gcctcgaaac aactgctggc agcggtaggg
     5581 cagagtcgac tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg
     5641 caaatgctgc tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac
     5701 accctgcgtg cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc
     5761 gctacggcag agattaaagt cggcgataac gataaccttt ctgcgctggc ggcgattctg
     5821 gcgggtgccg ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgacccg
     5881 cgcagcaatc cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc
     5941 gcgattgccg gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac caaattgcag
     6001 gccgctgacg tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg
     6061 ggcgttattg gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg
     6121 actccgcttg aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg
     6181 gtagatgaag gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc
     6241 attaaaagcg tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa
     6301 ggccgcgata tcgcccacgg cgtcagtcgt tacaacagcg atgcattacg ccgtattgcc
     6361 ggacaccact cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt
     6421 caccgtgatg acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg
     6481 ctgcgaagca agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc
     6541 tggaaaaaat cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc
     6601 aggatgttgc tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac
     6661 tgacgcccgc acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg
     6721 atccggtggg gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc
     6781 gtcgcgtacc gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg
     6841 atgtcgcttc gctgtgcctg aaaaccggta atgcggtgat cctgcgcggt ggcaaagaaa
     6901 cgtgtcgcac taacgctgca acggtggcgg tgattcagga cgccctgaaa tcctgcggct
     6961 taccggcggg tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc
     7021 tgcgtatgga taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac
     7081 tgtgccgtga acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt
     7141 acgttgatga aagtgtagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc
     7201 agcgtccgag cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata
     7261 gcttcctgcc cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg
     7321 cagctgcact ggcgcagttg caggcaggcc ctgcgaaggt ggttgctgtt aaagccgaag
     7381 agtatgacga tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg
     7441 atgccatcgc ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg
     7501 atatgcgcaa cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg
     7561 cctctacgcg ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca
     7621 cacaaaaact ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga
     7681 tcggcattgg tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca
     7741 tttgattcat aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt
     7801 cacgttcgtg aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc
     7861 gtaatgcgaa ggtcgtaggt tcgactccta ttatcggcac cattaagaaa attaataact
     7921 tagcttaact tctctaagaa attgtatctt ctgttatcac ttacaaacgc ctatacgttg
     7981 ttatgggctt tggtagtgta acacgtatgg gcgagtagtt cccaggctca acctcgtaac
     8041 aagttccttt gaagctggaa gacgtgccac cgtctctaaa ccttctacca aaactcttag
     8101 caagatgttc cacacctggc gcggtgtcca atgatgctac acgtcgtgag acgttgcaga
     8161 ggataaacag gctaaatgat gggaagagtt ttaagtgcaa tagtggatta aagcccacct
     8221 cttcaaaacc taacttgatc gggaaagaat caagctctag cacggtttcg tccaccggag
     8281 tacaacgggt gcctgataag agaagaactg ctaagacttc atagcttttg tgaaaaagcc
     8341 ctttttagtg gatacgccta cggcgcttat tttttggtat aaccatcaaa ggcgctttat
     8401 ggctcctatt gtcttgaaaa tgtatcttta cttgtattgt gttttatgcg tagtctccct
     8461 gtgtagtatg taacttgtta attttcatat gaatacagag gcacacgatg aaaaacattg
     8521 cagcaatcaa acgtaataac cgcaagattc acgctcgtaa gttcctgtct acgccagaag
     8581 gaaaagcctg gctagagcgt aaacagagag agaacgaaga aagaaaactc cttagtgagt
     8641 tgaaatggct tagagaggat ttctgaaaat ctccagaacg caacaggacg cattctaacg
     8701 ctgttttcat tactcggtat agggataggt gatgtgtgtt taatgcgtct gtgcgcttgt
     8761 tttgtgcctt attttgcatt ttcataatgc ggaagagaac acggaaaacc ttaaaattaa
     8821 ttattggggt atttaaggct attttcaggt gatttaaggg tattaaactt gatttaattg
     8881 attaaaatat aaacagataa ttatgtatct tattgattta taaggttatt tttaagtaaa
     8941 ggtaaataaa aaataaatag ccataaatag ctataaatac atcttaatac ctatatccct
     9001 tcccttatgg ctagattgcg agaggtgctt aacgcgattt tcagcgcccc ccgatctgga
     9061 aaatccagaa tccccaaata tcagcaagga attttcttgc gtaatcaaca tcgtttttac
     9121 ctattaaatc attacacaga aagcctttct gtaatgtctc tcactaaact ttcactaaac
     9181 acgagaatga tcggttttat gtcggatcag atgctagaaa cagcaccacg tctcacaagg
     9241 gctgtaagcg atgaaaccag tgtttatgcg ggtgcaggtc aaaatatagg ccaaaatcca
     9301 tttaacataa taaatgttat atgtgctaag gaacacatat aacgccttga attgatgtat
     9361 cagtgg
//
LOCUS       DAJTVK010000382         5558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.guided.195, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000382 DAJTVK010000000
VERSION     DAJTVK010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.guided.195"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            562..801
                     /locus_tag="OO372_004568"
     CDS             562..801
                     /locus_tag="OO372_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6343833.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(989..1966)
                     /gene="intI2"
                     /locus_tag="OO372_004569"
                     /pseudo
     CDS             complement(989..1966)
                     /gene="intI2"
                     /locus_tag="OO372_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2298..2771
                     /locus_tag="OO372_004570"
     CDS             2298..2771
                     /locus_tag="OO372_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6343834.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2866..3390
                     /gene="sat2"
                     /locus_tag="OO372_004571"
     CDS             2866..3390
                     /gene="sat2"
                     /locus_tag="OO372_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6343835.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3448..4236
                     /gene="aadA1"
                     /locus_tag="OO372_004572"
     CDS             3448..4236
                     /gene="aadA1"
                     /locus_tag="OO372_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6343836.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            4312..4809
                     /locus_tag="OO372_004573"
     CDS             4312..4809
                     /locus_tag="OO372_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343837.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4870..5241
                     /locus_tag="OO372_004574"
     CDS             4870..5241
                     /locus_tag="OO372_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343838.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
BASE COUNT         1549 a         1243 c         1323 g         1443 t
ORIGIN      
        1 ggatcggtca actgatcctt aactgatcgg cattacaccg gtcggcagcc cttgtgggcg
       61 gacaaaatag ttgggaactg ggaggggtgg aaatggagtt tttaaggatt atttagggaa
      121 gagtgacaaa atagatggga actgggtgta gcgtcgtaag ctaatacgaa aattaaaaat
      181 gacaaaatag tttggaacta gatttcactt atctggttaa ctaatctagc taacctgccg
      241 ctcagcttag tacgatccta tatccaactg atcataatcc tttcatcagt atcccaaatt
      301 aaatccccct cctagactgc ctcaccaaca ctttacagtg atcccgtatt tccggacagg
      361 tgattgattt ttttcgcttg tacaggtgtt aagccacagt tatcttgatg tggacattat
      421 gcattgtaat agcccattaa catgcgctaa gagcataaat taatatacag taacaagcat
      481 tacgaggtgc taatgtttgg aaagcagcct cttccaacta caataataag ttaactatcc
      541 tccacccaca aataattaca aatgacccct ttccgctata actccgatct caccagcggc
      601 tcgctacaaa cccgagagtg tcgcattatt actggcttgt tactccaaga gcttgatgaa
      661 gctgcctggg ataaagccat gtataaggaa aatgtgcttc agaaacgcac gcaatctacg
      721 gtaagacgta tttcttcagc gcttagaaaa cgcctagaac acctaagctc tgacttttgg
      781 gcttttgcgt ttttatgtta ggcattgtct ggcaactgga ggaagtgtaa tgaccatgaa
      841 tggtcactgt gattgacagt atcttgctta aacttatctg aacaatggta gaactggctc
      901 atatttatcc cctctatgat tgtttatctt tttcaccata aaaagtatca ggggctactc
      961 agggctacat aaagatattt atctactttt actgattgat aagtagcatc agtccatccg
     1021 caggactggt ggtgccggca aaatgctgac ccaacacatg cgtatagatt tgcgtggtct
     1081 taacatcgtt atgccctaag agttcttgca cagtgcgaat atcacgcccc gcttgtaata
     1141 gatgcgtagc aaacgagtga cgaaatgtat gacaagtgac acgcttgcta acgatgcctg
     1201 ctttttgtac ggctgccttc aatgcctttc gcgcaacgga gtcatgcaga tgatggcggc
     1261 ataatttgcc gttatacggg tggttgcaga gcgtgctgga gggaaagaca aacatccacg
     1321 ccgcttgtcg ataagcagaa gggtatttgt gatctaaagc aaaaggcagc gatggcccta
     1381 cgccttgtaa gttgtcgtct tgctgaataa gccgcgcttg ctcaatgagt tattttattg
     1441 ctgggattag gcgcgtgggc agtaggctgt ttctgctttt cccaccctta ccgtcatgca
     1501 cagtgatgca gccattatca aaatcaaaat ctttaacccg caaacgcaag cattcattaa
     1561 tgcgcaaacc tgcaccatac agcagcgtaa aaataacttg gttgcgagta tccataacct
     1621 gcaaaatgcg ttgcacttca tttgcagaga taacagaggg tagccgtcta ggcttgcttg
     1681 cagggatata atcaatatcg cccaacggct gttgtaaaaa cctgttgtac aaaaaagcta
     1741 gggcatttaa agcgattttc tgcgtgttta tggctacatg tctgctgttt gctaagctgg
     1801 ataaaaacag cctgacctct tcactgccca tggtctgagg atgacgtttt ttgtgaaaca
     1861 gaataaaacg cttaatccag tgcaggtaag ttttttcagt tttcagcgca taaccttttt
     1921 gtcgcatatc cgtgcgtata gaatttaaaa atggactgtt agacataaaa cgctccttgt
     1981 cttgcaactg tctgcctata cagcctattc tagctgggat ttaaaaaagt gcctgttttt
     2041 tacgcctaga gatgcttgtt taccggtaga gttttaattt aatgctaaat aaattaaaat
     2101 gttatgagtt ctttgggtga gataatgtgc atcgtgcaag caggatagac ggcatgcacg
     2161 atttgtaata acagagtgtc ttgtattttt aaagaaagtc tatttaatac aagtgattat
     2221 attaattaac ggtaagcatc agcgggtgac aaaacgagca tgcttactaa taaaatgtta
     2281 acctctgagg aagaattgtg aaactatcac taatggtagc tatatcgaag aatggagtta
     2341 tcgggaatgg ccctgatatt ccatggagtg ccaaaggtga acagctcctg tttaaagcta
     2401 ttacctataa ccaatggctg ttggttggac gcaagacttt tgaatcaatg ggagcattac
     2461 ccaaccgaaa gtatgcggtc gtaacacgtt caagttttac atctgacaat gagaacgtat
     2521 tgatctttcc atcaattaaa gatgctttaa ccaacctaaa gaaaataacg gatcatgtca
     2581 ttgtttcagg tggtggggag atatacaaaa gcctgatcga tcaagtagat acactacata
     2641 tatctacaat agacatcgag ccggaaggtg atgtttactt tcctgaaatc cccagcaatt
     2701 ttaggccagt ttttacccaa gacttcgcct ctaacataaa ttatagttac caaatctggc
     2761 aaaagggtta acaagtggca gcaacggatt cgcaaacctg tcacgccttt tgtaccaaaa
     2821 gccgcgccag gtttgcgatc cgctgtgcca ggcgttaggc gtcatatgaa gatttcggtg
     2881 atccctgagc aggtggcgga aacattggat gctgagaacc atttcattgt tcgtgaagtg
     2941 ttcgatgtgc acctatccga ccaaggcttt gaactatcta ccagaagtgt gagcccctac
     3001 cggaaggatt acatctcgga tgatgactct gatgaagact ctgcttgcta tggcgcattc
     3061 atcgaccaag agcttgtcgg gaagattgaa ctcaactcaa catggaacga tctagcctct
     3121 atcgaacaca ttgttgtgtc gcacacgcac cgaggcaaag gagtcgcgca cagtctcatc
     3181 gaatttgcga aaaagtgggc actaagcaga cagctccttg gcatacgatt agagacacaa
     3241 acgaacaatg tacctgcctg caatttgtac gcaaaatgtg gctttactct cggcggcatt
     3301 gacctgttca cgtataaaac tagacctcaa gtctcgaacg aaacagcgat gtactggtac
     3361 tggttctcgg gagcacagga tgacgcctaa caattcattc aagccgacac cgcttcgcgg
     3421 cgcggcttaa ttcaggagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     3481 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     3541 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     3601 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     3661 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     3721 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     3781 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     3841 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     3901 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     3961 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     4021 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     4081 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag gcaggcttat
     4141 cttggacaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgttcactac
     4201 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg ttcaagccga
     4261 cgccgcttcg cggcgcggct taactcaagc gttagagagc tggggaagac tatgcgcgat
     4321 ctgttgaagg tggttctaag cctcgtactt gcgatggcat cggggcaggc acttgctgac
     4381 ctgccaattg ttttagtgga tgaagctcgt cttccctatg actactcccc atccaactac
     4441 gacatttctc caagcaacta cgacaactcc ataagcaatt acgacaatag tccatcaaat
     4501 tacgacaact ctgagagcaa ctacgataat agttcatcca attacgacaa tagtcgcaac
     4561 ggaaatcgta ggcttatata tagcgcaaat gggtctcgca ctttcgccgg ctactacgtc
     4621 attgccaaca atgggacaac gaacttcttt tccacatctg gcaaaaggat gttctacacc
     4681 ccaaaagggg ggcgcggcgt ctatggcggc aaagatggga gcttctgcgg ggcattggtc
     4741 gtcataaatg gccaattttc gcttgccctg acagataacg gcctgaagat catgtatcta
     4801 agcaactagc ctgctctcta ataaaatgtt aggcctcaac atctagtcgc aagctgaggg
     4861 gaaccactag tgtcatacga acctccaaga gacggttaca caaacgggta cattgttgat
     4921 gtcatgtatg acaatcgccc aagtaagtat ccagctgtgt tcagaacgta cgtccgaatt
     4981 ccagactatg tcgaactaga taacgcggag gagctggccg acgcaatcat tcgagaggtt
     5041 gagttaaagg aggcatggct cacaagggtc ttggggaact cagccgatcc tgacgcttct
     5101 gtgggcaagt gggaccggcg cctagcgcag tgcggtggta ttgatgagat gtcaagtggg
     5161 gtgcaggcaa catacttcag tccaaacggt cagctggtcg gcttagtggc atacattgat
     5221 gccgaatgga tggatgaata gctctgacgt tacgattcag gtgagacaac cagatgggca
     5281 ttgcgtacaa gctcagttgc caccgatgtg gctacgagtc tgatcttctt tacctaggcc
     5341 aaggtatggc gatgcttcct gagcaaatag tcggaacgtg cacatgctgc gacaatctaa
     5401 cgacgatttc tataaatgaa agctacggag tatgctcact ttgtggggct aaggatcgtg
     5461 tgatatttac aagctatcag catgaggcaa ggccaagaaa tccctgctat ctagagagaa
     5521 gtagactggc cccctgaatc tccagacaac cagtatca
//
LOCUS       DAJTVK010000383         3829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.guided.238, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000383 DAJTVK010000000
VERSION     DAJTVK010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.guided.238"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            <1..183
                     /locus_tag="OO372_004575"
     CDS             <1..183
                     /locus_tag="OO372_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCR6343839.1"
                     /translation="TRATAHERTPQQERQKAAREAERGREAWTLGQGMKKPVAGCYGR
                     LTRWKGGGDVVYMALL"
     gene            complement(215..865)
                     /gene="tetR(A)"
                     /locus_tag="OO372_004576"
     CDS             complement(215..865)
                     /gene="tetR(A)"
                     /locus_tag="OO372_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCR6343840.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            971..2170
                     /gene="tet(A)"
                     /locus_tag="OO372_004577"
     CDS             971..2170
                     /gene="tet(A)"
                     /locus_tag="OO372_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCR6343841.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(2137..2325)
                     /locus_tag="OO372_004578"
                     /pseudo
     CDS             complement(2137..2325)
                     /locus_tag="OO372_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2369..2758)
                     /locus_tag="OO372_004579"
     CDS             complement(2369..2758)
                     /locus_tag="OO372_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6343842.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2976..3416
                     /locus_tag="OO372_004580"
     CDS             2976..3416
                     /locus_tag="OO372_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR6343843.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          808 a         1097 c         1083 g          841 t
ORIGIN      
        1 acccgcgcca cggcccacga gcggacgccg cagcaggagc gccagaaggc cgccagagag
       61 gccgagcgcg gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc
      121 tgctacgggc gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg
      181 tagtgagtgg gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt
      241 caacgttcct gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct
      301 gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg
      361 gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct
      421 gctcctcaag cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt
      481 ccccggccga aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct
      541 gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg
      601 cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg
      661 gcaccgaatg cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc
      721 gcttgttcct gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt
      781 tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca
      841 ctgtattcgg ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc
      901 caccaactta tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg
      961 gaggccagac gtgaaaccca acagacccct gatcgtaatt ctgagcactg tcgcgctcga
     1021 cgctgtcggc atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca
     1081 ctcgaacgac gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tgatgcaatt
     1141 tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc cggtcttgct
     1201 cgtctcgctg gccggcgctg ctgtcgacta cgccatcatg gcgacggcgc ctttcctttg
     1261 ggttctctat atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg
     1321 cgcttatatt gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag
     1381 cgcctgtttc gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt
     1441 ctccccccac gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg
     1501 ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct
     1561 caacccgctc gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc
     1621 ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg
     1681 cgaggatcgc tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat
     1741 tctgcattca ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag
     1801 gcgggcactc atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc
     1861 gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat
     1921 gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca
     1981 aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc
     2041 gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct
     2101 ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agaaattctt caaattcccg
     2161 ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga atgccgggta
     2221 atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct ggtaacgacc
     2281 agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc gcgtccttgc
     2341 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa
     2401 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccactctt acattgttcg
     2461 cagtatctag gtacatgtta ttgcgtgcac cttcatgatg tataatgtta ttaatgacag
     2521 cagaaagatc gacagaatta atattcttcg tacctgggac agttatcggg tgtcggccag
     2581 tgatgagacc aaaggatgca gtataagatg aaccactact attatcatgg ccaccattac
     2641 caccaccgtt aaaactacct cccgaacttc cagagccgct atgaccatca ttgtaggggt
     2701 catttggtcc catgtgacca aaaccaccga cagtaccgct atttaactct gtactcataa
     2761 aattatcctc tttgactttt aaaaacagca aaaactgtat ttataacaat gtgtttatat
     2821 acagtattcg aaatactacg cccgtcaaaa ccacaccgtc aacaggtttt ttgtgtattt
     2881 ttcacgcctc acgggcagcc tcagaccttt tgtgttaaaa cagtaatatg ttctgctgtt
     2941 acaatacggt acacacagga gacactgtca cttttatggc atggcacaca gttcaagaga
     3001 cactgaaatt aacaggaaag tcgcgctcgc aattatatcg tgacatggct aaaggccttg
     3061 tcagctatcg cacaggaaaa aatgatcgcc gggagttcga gaccagtgaa ctgattcggg
     3121 cctatggcga gctgcgcact tctgagacac caaaaaggca caaggaggga cactccaaat
     3181 ctgtcgttag ccaggcggaa aatgctcagc tagacgtcat tcagcagaaa ctggacgctc
     3241 tgcagcagac tgttacccta atgcttgaag acaagatggc gcgggaagca gaaaaccgcc
     3301 tgcgaaatga ggagagggaa caactacagg ccgaaattag ccgtctggct gacgcgctgg
     3361 cacaggaaaa aaagcgggga ttctggtcgc ggctgtttcg ctccggttca gagtaacagt
     3421 gcaggtgact ctgatttacc ataacatctg ttatcagcac cgcctgagag gccctcaggg
     3481 cgtttctgac gggaaaccgg taaaacaggc aacaaaccac ccgaaaaccc gccagaaacg
     3541 cgcaggctct gtttttacgg catgcatgag tatgcgcaca agtgcatagc gcggaaattc
     3601 acaccattca gccggggtaa gtcctgtttc cggcgttctt cccatccgaa aacggtgaac
     3661 ggggacgcaa tttattgcgt ggccgtgaag cgttgccggc gcagccggaa aacccgctgt
     3721 cagcacgttc aggaaggggg tgatgtacaa atggcacaga ggatgaaagt gacgtatgag
     3781 ctgaaggaat gatgtaaaag tgatgtataa gtgatgtaaa agtgatgta
//
LOCUS       DAJTVK010000384         3478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.guided.246, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000384 DAJTVK010000000
VERSION     DAJTVK010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.guided.246"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            607..1422
                     /gene="sul2"
                     /locus_tag="OO372_004581"
     CDS             607..1422
                     /gene="sul2"
                     /locus_tag="OO372_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6343844.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1483..2286
                     /locus_tag="OO372_004582"
                     /note="aph(3'')-Ib"
     CDS             1483..2286
                     /locus_tag="OO372_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR6343845.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2286..3122
                     /locus_tag="OO372_004583"
     CDS             2286..3122
                     /locus_tag="OO372_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6343846.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          753 a          965 c          966 g          794 t
ORIGIN      
        1 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
       61 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
      121 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      181 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      241 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      301 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      361 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      421 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      481 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      541 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      601 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      661 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      721 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      781 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      841 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      901 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      961 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1021 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1081 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1141 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1201 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1261 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1321 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1381 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1441 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1501 ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt
     1561 gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt
     1621 gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc
     1681 gaggtcatca actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg
     1741 ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg
     1801 cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga gcgcaggctg
     1861 tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc
     1921 ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag
     1981 ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc
     2041 atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg
     2101 cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag
     2161 aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg
     2221 atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg
     2281 ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca
     2341 ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa
     2401 tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc
     2461 tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt
     2521 tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc
     2581 aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc
     2641 tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg
     2701 atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa
     2761 tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca
     2821 tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct
     2881 ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta
     2941 gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc
     3001 tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag
     3061 aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact
     3121 agatatcaag cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc
     3181 tggccaaagc cttagcgtag gattccgccc cttcccgcaa acgacccctg gaagccccgt
     3241 agcgacgcgg agaggggcga gacaagccaa gggcgcaggc tcgatgcgca gcacgacata
     3301 gccggttctc gcaaggacga gaatttccct gcggtgcccc tcaagtgtca atgaaagttt
     3361 ccaacgcgag ccattcgcga gagccttgag tccacgctag atctatctca tctgcgcaag
     3421 gcagaacgtg aagacggccg ccctggacct cgcccgcgag cgccaggcgc acgaggcc
//
LOCUS       DAJTVK010000385         3477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella sonnei isolate Stool culture
            SAMEA110466778-rid15817043.guided.247, whole genome shotgun
            sequence.
ACCESSION   DAJTVK010000385 DAJTVK010000000
VERSION     DAJTVK010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA110466778
            Sequence Read Archive: ERR10074644
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477310.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477310.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:40:25
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,586
            CDSs (total)                      :: 4,483
            Genes (coding)                    :: 4,222
            CDSs (with protein)               :: 4,222
            Genes (RNA)                       :: 103
            rRNAs                             :: 2, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 3 (16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 122 of 261
            Pseudo Genes (incomplete)         :: 73 of 261
            Pseudo Genes (internal stop)      :: 94 of 261
            Pseudo Genes (multiple problems)  :: 27 of 261
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA110466778-rid15817043.guided.247"
                     /isolate="Stool culture"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="South Africa"
                     /collection_date="2015-04-21"
                     /collected_by="The Group for Enteric respiratory and
                     meningeal disease surveillance - South Africa"
     gene            607..1422
                     /gene="sul2"
                     /locus_tag="OO372_004584"
     CDS             607..1422
                     /gene="sul2"
                     /locus_tag="OO372_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR6343847.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1527..2285
                     /locus_tag="OO372_004585"
     CDS             1527..2285
                     /locus_tag="OO372_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR6343848.1"
                     /translation="MPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGERDRLIWLK
                     GRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLGAVHSLSV
                     DQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELPVRLDQER
                     TDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEENWAAPDE
                     AERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2285..3121
                     /locus_tag="OO372_004586"
     CDS             2285..3121
                     /locus_tag="OO372_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR6343849.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          753 a          965 c          966 g          793 t
ORIGIN      
        1 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
       61 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
      121 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
      181 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
      241 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
      301 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
      361 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
      421 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
      481 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
      541 gattcccttt cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa
      601 gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc
      661 tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg
      721 gccgaggggg cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct
      781 gtttcgtccg acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat
      841 ggcattcccg tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt
      901 ggtgtggcct atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg
      961 gcgaaatcat ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg
     1021 cgcgaggcac ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc
     1081 gcggcgctga cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg
     1141 ttttttctgg gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg
     1201 cggctgcgct tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg
     1261 ctcacaggcc gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc
     1321 gccgcaggtg gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg
     1381 gcggtattgg cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt
     1441 tctctatatt cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt
     1501 tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct ggtgattttg
     1561 tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg
     1621 agctcgctgg agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg
     1681 aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt ggtgataacg gcaattccgg
     1741 gagtaccggc ggctgatctg tctggagcgg atttgctcaa agcgtggccg tcaatggggc
     1801 agcaacttgg cgctgttcac agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt
     1861 cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct
     1921 taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc gaacgagagc
     1981 taccggtgcg gctcgaccaa gagcgcaccg atatggttgt ttgccatggt gatccctgca
     2041 tgccgaactt catggtggac cctaaaactc ttcaatgcac gggtctgatc gaccttgggc
     2101 ggctcggaac agcagatcgc tatgccgatt tggcactcat gattgctaac gccgaagaga
     2161 actgggcagc gccagatgaa gcagagcgcg ccttcgctgt cctattcaat gtattgggga
     2221 tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg attggaccct ctgacttggg
     2281 gttgatgttc atgccgcctg tttttcctgc tcattggcac gtttcgcaac ctgttctcat
     2341 tgcggacacc ttttccagcc tcgtttggaa agtttcattg ccagacggga ctcctgcaat
     2401 cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa ctgcgcgggg ccgactatct
     2461 ggtatggcgc aatgggaggg gagcagtccg gttgctcggt cgtgagaaca atctgatgtt
     2521 gctcgaatat gccggggagc gaatgctctc tcacatcgtt gccgagcacg gcgactacca
     2581 ggcgaccgaa attgcagcgg aactaatggc gaagctgtat gccgcatctg aggaacccct
     2641 gccttctgcc cttctcccga tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga
     2701 tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg gcgattatag ccgatcaaat
     2761 gatgagcaat gcctcggaac tgcgtgggct acatggcgat ctgcatcatg aaaacatcat
     2821 gttctccagt cgcggctggc tggtgataga tcccgtcggt ctggtcggtg aagtgggctt
     2881 tggcgccgcc aatatgttct acgatccggc tgacagagac gacctttgtc tcgatcctag
     2941 acgcattgca cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct
     3001 gctcgaccag gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg atggagaaga
     3061 ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta
     3121 gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct
     3181 ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgacccctgg aagccccgta
     3241 gcgacgcgga gaggggcgag acaagccaag ggcgcaggct cgatgcgcag cacgacatag
     3301 ccggttctcg caaggacgag aatttccctg cggtgcccct caagtgtcaa tgaaagtttc
     3361 caacgcgagc cattcgcgag agccttgagt ccacgctaga tctatctcat ctgcgcaagg
     3421 cagaacgtga agacggccgc cctggacctc gcccgcgagc gccaggcgca cgaggcc
//
